SHD cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	SHD
Entrez Gene ID	828520
Full Name	Chaperone protein htpG family protein
Synonyms	AtHsp90-7,AtHsp90.7,HEAT SHOCK PROTEIN 90-7,HEAT SHOCK PROTEIN 90.7,HSP90.7,SHEPHERD,T22A6.20,T22A6_20
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_202877.1	NP_974606.1	Chaperone protein htpG family protein
NM_118552.4	NP_194150.1	Chaperone protein htpG family protein

Pathways from BioSystems

Homologies

	HSP90B1	XP_001095189.1
Mus musculus (house mouse)	Hsp90b1	NP_035761.1
Rattus norvegicus (Norway rat)	Hsp90b1	NP_001012197.2
Homo sapiens (human)	HSP90B1	NP_003290.1
Canis lupus familiaris (dog)	HSP90B1	NP_001003327.1
Bos taurus (cattle)	HSP90B1	NP_777125.1
Oryza sativa (rice)	Os06g0716700	NP_001058590.1
Gallus gallus (chicken)	HSP90B1	NP_989620.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001424	XP_321706.5
Caenorhabditis elegans (roundworm)	CELE_T05E11.3	NP_001255536.1
Danio rerio (zebrafish)	hsp90b1	NP_937853.1
Drosophila melanogaster (fruit fly)	Gp93	NP_651601.1
Arabidopsis thaliana (thale cress)	SHD	NP_974606.1
Xenopus tropicalis (tropical clawed frog)	hsp90b1	NP_001039228.1
Pan troglodytes (chimpanzee)	HSP90B1	NP_001233416.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Seedlings expressing HSP90.7(Delta22) had enhanced sensitivity to ER stress induced by tunicamycin or high concentration of calcium. Its general chaperone activity in preventing the model protein from heat-induced aggregation was not significantly affected.
Title: A highly charged region in the middle domain of plant endoplasmic reticulum (ER)-localized heat-shock protein 90 is required for resistance to tunicamycin or high calcium-induced ER stresses.

Results show that the induction expression of ABA-responsive genes under salt and drought stress was delayed by constitutive overexpression of cytosolic AtHsp90.2, but was hardly affected by that of organellar AtHsp90.5 and AtHsp90.7.
Title: Overexpression of AtHsp90.2, AtHsp90.5 and AtHsp90.7 in Arabidopsis thaliana enhances plant sensitivity to salt and drought stresses.

The following SHD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb16133	NM_118552.4 Latest version!	Arabidopsis thaliana Chaperone protein htpG family protein (SHD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb16134	NM_202877.1 Latest version!	Arabidopsis thaliana Chaperone protein htpG family protein (SHD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SHD ( NM_118552.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_194150.1 Arabidopsis thaliana Chaperone protein htpG family protein (SHD), mRNA.

Price & Availability↑

CloneID	OAb16133
Clone ID Related Accession (Same CDS sequence)	NM_118552.4
Accession Version	NM_118552.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Chaperone protein htpG family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_118552.3.

ATGAGGAAGA GGACGCTCGT GTCCGTTTTG TTCCTTTTCT CGCTTCTGTT TCTTCTTCCA 
GATCAAGGAA GAAAGTTACA CGCGAATGCA GAAGAGAGTT CCGATGATGT TACAGATCCA 
CCAAAAGTTG AGGAGAAAAT TGGTGGTCAC GGTGGTTTAT CGACGGATTC CGATGTAGTT 
CATAGAGAGT CTGAGTCGAT GTCAAAGAAG ACGCTTCGCA GTAACGCGGA GAAGTTTGAG 
TTCCAAGCTG AGGTTTCTCG GCTTATGGAC ATTATTATCA ACTCTCTATA CAGTAACAAG 
GACATTTTCT TAAGAGAGTT GATCTCCAAT GCTTCTGATG CCTTGGACAA AATTAGGTTC 
CTTGCACTCA CTGACAAAGA CGTTTTGGGT GAAGGTGACA CTGCTAAGCT TGAGATCCAG 
ATTAAGCTAG ACAAAGCCAA GAAGATTCTT TCTATTCGAG ACCGGGGAAT TGGTATGACT 
AAGGAAGACT TAATCAAGAA CCTTGGTACC ATAGCAAAAT CTGGAACGTC AGCTTTTGTT 
GAGAAAATGC AATCAAGTGG GGATTTGAAC CTTATTGGAC AATTTGGAGT TGGATTCTAC 
TCTGCCTATC TTGTTGCTGA CTACATTGAA GTCATTAGCA AGCACAATGA TGACAGCCAG 
TATGTATGGG AATCAAAGGC TAACGGTAAA TTTGCTGTTT CCGAGGATAC ATGGAATGAG 
CCTCTTGGAC GTGGAACCGA GATTAGATTA CATCTTAGGG ATGAAGCTGG CGAGTACCTA 
GAAGAAAGTA AACTTAAGGA ATTGGTGAAG AGATATTCTG AATTCATTAA CTTCCCTATC 
TCCCTCTGGG CTAGCAAAGA GGTTGAAACC GAGGTCCCAG TTGAGGAAGA TGAATCAGCT 
GATGAGGAAA CTGAAACCAC CTCTACCGAG GAAGAAAAGG AAGAAGATGC TGAGGAGGAA 
GATGGTGAGA AAAAGCAGAA AACCAAGAAG GTGAAAGAAA CAGTTTATGA ATGGGAGCTT 
CTGAATGATG TTAAGGCTAT ATGGCTGAGA AGTCCAAAGG AGGTGACAGA GGAAGAGTAC 
ACTAAATTCT ACCACTCTCT CTCAAAGGAT TTTACCGACG AGAAGCCAAT GGCTTGGAGT 
CACTTTAATG CTGAAGGTGA TGTTGAATTC AAGGCTGTGT TGTATGTTCC TCCGAAAGCT 
CCCCATGATC TATACGAGAG CTATTACAAC AGCAACAAGG CGAACTTGAA GCTATATGTC 
AGGAGGGTGT TCATCTCAGA TGAATTTGAT GAGCTTCTGC CTAAGTATTT GAGTTTCTTG 
AAGGGTCTTG TTGACTCTGA CACATTGCCT CTAAATGTAT CAAGAGAAAT GCTTCAACAA 
CACAGCAGCT TAAAGACAAT TAAGAAGAAG CTTATCCGAA AGGCGCTTGA TATGATCCGC 
AAGCTTGCTG AAGAAGATCC TGATGAAATC CATGATGATG AGAAAAAAGA TGTGGAGAAG 
TCTGGTGAGA ACGATGAGAA GAAGGGTCAA TACACAAAAT TCTGGAACGA GTTTGGCAAG 
TCTGTCAAAC TAGGTATCAT TGAGGATGCT GCTAACAGGA ACCGCTTGGC AAAACTTCTT 
CGTTTTGAGA CAACAAAGTC CGATGGAAAA TTGACTTCCC TGGATCAGTA CATTAAGAGA 
ATGAAAAAGA GCCAAAAGGA CATATTTTAC ATCACAGGAA GCAGCAAGGA ACAGCTAGAG 
AAATCTCCAT TCCTGGAGAG GCTCATCAAG AAGGGCTATG AGGTCATCTT CTTCACAGAC 
CCAGTTGATG AATACTTGAT GCAATACCTG ATGGATTACG AAGACAAAAA GTTCCAGAAT 
GTGTCCAAGG AAGGACTGAA GGTTGGGAAA GATTCAAAAG ACAAGGAGCT GAAGGAAGCA 
TTCAAGGAGC TAACAAAGTG GTGGAAAGGA AATCTCGCTA GCGAAAACGT AGACGATGTC 
AAAATCAGCA ACCGTTTGGC TGACACTCCA TGTGTAGTCG TAACATCCAA GTTTGGATGG 
AGTGCTAATA TGGAGAGGAT CATGCAGTCC CAAACTCTCT CGGATGCTAA TAAGCAAGCT 
TACATGCGCG GAAAGAGAGT CCTCGAGATC AACCCACGAC ACCCTATCAT CAAAGAACTC 
AAGGATAGAA TTGCAAGTGA CCCAGAGGAT GAGAGTGTGA AGGAAACAGC ACAGCTCATG 
TACCAGACAG CTTTGATCGA GAGTGGATTC ATACTCACCG ACCCAAAAGA CTTTGCTGCC 
CGTATTTATA ACTCAGTCAA GAGCGGTCTG AACATCAGCC CTGATGCAGT AGCCGACGAG 
GAAATCGAGG CAGCAGAGGA ACCAGAGACC AGTGAGGCAA CTGAGACGAA ATCAGATGAC 
TTGGCTGGTG GTCTAAACAT TGAAGCCGAA CCCGTTGAGC AACAAGAAGA GAACACCAAG 
GACGAACTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_194150.1
CDS	208..2679
Translation	MRKRTLVSVLFLFSLLFLLPDQGRKLHANAEESSDDVTDPPKVEEKIGGHGGLSTDSDVVHRESESMSKKTLRSNAEKFEFQAEVSRLMDIIINSLYSNKDIFLRELISNASDALDKIRFLALTDKDVLGEGDTAKLEIQIKLDKAKKILSIRDRGIGMTKEDLIKNLGTIAKSGTSAFVEKMQSSGDLNLIGQFGVGFYSAYLVADYIEVISKHNDDSQYVWESKANGKFAVSEDTWNEPLGRGTEIRLHLRDEAGEYLEESKLKELVKRYSEFINFPISLWASKEVETEVPVEEDESADEETETTSTEEEKEEDAEEEDGEKKQKTKKVKETVYEWELLNDVKAIWLRSPKEVTEEEYTKFYHSLSKDFTDEKPMAWSHFNAEGDVEFKAVLYVPPKAPHDLYESYYNSNKANLKLYVRRVFISDEFDELLPKYLSFLKGLVDSDTLPLNVSREMLQQHSSLKTIKKKLIRKALDMIRKLAEEDPDEIHDDEKKDVEKSGENDEKKGQYTKFWNEFGKSVKLGIIEDAANRNRLAKLLRFETTKSDGKLTSLDQYIKRMKKSQKDIFYITGSSKEQLEKSPFLERLIKKGYEVIFFTDPVDEYLMQYLMDYEDKKFQNVSKEGLKVGKDSKDKELKEAFKELTKWWKGNLASENVDDVKISNRLADTPCVVVTSKFGWSANMERIMQSQTLSDANKQAYMRGKRVLEINPRHPIIKELKDRIASDPEDESVKETAQLMYQTALIESGFILTDPKDFAARIYNSVKSGLNISPDAVADEEIEAAEEPETSEATETKSDDLAGGLNIEAEPVEQQEENTKDEL

Target ORF information:

RefSeq Version	NM_118552.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Chaperone protein htpG family protein (SHD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118552.4

ORF Insert Sequence:

ATGAGGAAGA GGACGCTCGT GTCCGTTTTG TTCCTTTTCT CGCTTCTGTT TCTTCTTCCA 
GATCAAGGAA GAAAGTTACA CGCGAATGCA GAAGAGAGTT CCGATGATGT TACAGATCCA 
CCAAAAGTTG AGGAGAAAAT TGGTGGTCAC GGTGGTTTAT CGACGGATTC CGATGTAGTT 
CATAGAGAGT CTGAGTCGAT GTCAAAGAAG ACGCTTCGCA GTAACGCGGA GAAGTTTGAG 
TTCCAAGCTG AGGTTTCTCG GCTTATGGAC ATTATTATCA ACTCTCTATA CAGTAACAAG 
GACATTTTCT TAAGAGAGTT GATCTCCAAT GCTTCTGATG CCTTGGACAA AATTAGGTTC 
CTTGCACTCA CTGACAAAGA CGTTTTGGGT GAAGGTGACA CTGCTAAGCT TGAGATCCAG 
ATTAAGCTAG ACAAAGCCAA GAAGATTCTT TCTATTCGAG ACCGGGGAAT TGGTATGACT 
AAGGAAGACT TAATCAAGAA CCTTGGTACC ATAGCAAAAT CTGGAACGTC AGCTTTTGTT 
GAGAAAATGC AATCAAGTGG GGATTTGAAC CTTATTGGAC AATTTGGAGT TGGATTCTAC 
TCTGCCTATC TTGTTGCTGA CTACATTGAA GTCATTAGCA AGCACAATGA TGACAGCCAG 
TATGTATGGG AATCAAAGGC TAACGGTAAA TTTGCTGTTT CCGAGGATAC ATGGAATGAG 
CCTCTTGGAC GTGGAACCGA GATTAGATTA CATCTTAGGG ATGAAGCTGG CGAGTACCTA 
GAAGAAAGTA AACTTAAGGA ATTGGTGAAG AGATATTCTG AATTCATTAA CTTCCCTATC 
TCCCTCTGGG CTAGCAAAGA GGTTGAAACC GAGGTCCCAG TTGAGGAAGA TGAATCAGCT 
GATGAGGAAA CTGAAACCAC CTCTACCGAG GAAGAAAAGG AAGAAGATGC TGAGGAGGAA 
GATGGTGAGA AAAAGCAGAA AACCAAGAAG GTGAAAGAAA CAGTTTATGA ATGGGAGCTT 
CTGAATGATG TTAAGGCTAT ATGGCTGAGA AGTCCAAAGG AGGTGACAGA GGAAGAGTAC 
ACTAAATTCT ACCACTCTCT CTCAAAGGAT TTTACCGACG AGAAGCCAAT GGCTTGGAGT 
CACTTTAATG CTGAAGGTGA TGTTGAATTC AAGGCTGTGT TGTATGTTCC TCCGAAAGCT 
CCCCATGATC TATACGAGAG CTATTACAAC AGCAACAAGG CGAACTTGAA GCTATATGTC 
AGGAGGGTGT TCATCTCAGA TGAATTTGAT GAGCTTCTGC CTAAGTATTT GAGTTTCTTG 
AAGGGTCTTG TTGACTCTGA CACATTGCCT CTAAATGTAT CAAGAGAAAT GCTTCAACAA 
CACAGCAGCT TAAAGACAAT TAAGAAGAAG CTTATCCGAA AGGCGCTTGA TATGATCCGC 
AAGCTTGCTG AAGAAGATCC TGATGAAATC CATGATGATG AGAAAAAAGA TGTGGAGAAG 
TCTGGTGAGA ACGATGAGAA GAAGGGTCAA TACACAAAAT TCTGGAACGA GTTTGGCAAG 
TCTGTCAAAC TAGGTATCAT TGAGGATGCT GCTAACAGGA ACCGCTTGGC AAAACTTCTT 
CGTTTTGAGA CAACAAAGTC CGATGGAAAA TTGACTTCCC TGGATCAGTA CATTAAGAGA 
ATGAAAAAGA GCCAAAAGGA CATATTTTAC ATCACAGGAA GCAGCAAGGA ACAGCTAGAG 
AAATCTCCAT TCCTGGAGAG GCTCATCAAG AAGGGCTATG AGGTCATCTT CTTCACAGAC 
CCAGTTGATG AATACTTGAT GCAATACCTG ATGGATTACG AAGACAAAAA GTTCCAGAAT 
GTGTCCAAGG AAGGACTGAA GGTTGGGAAA GATTCAAAAG ACAAGGAGCT GAAGGAAGCA 
TTCAAGGAGC TAACAAAGTG GTGGAAAGGA AATCTCGCTA GCGAAAACGT AGACGATGTC 
AAAATCAGCA ACCGTTTGGC TGACACTCCA TGTGTAGTCG TAACATCCAA GTTTGGATGG 
AGTGCTAATA TGGAGAGGAT CATGCAGTCC CAAACTCTCT CGGATGCTAA TAAGCAAGCT 
TACATGCGCG GAAAGAGAGT CCTCGAGATC AACCCACGAC ACCCTATCAT CAAAGAACTC 
AAGGATAGAA TTGCAAGTGA CCCAGAGGAT GAGAGTGTGA AGGAAACAGC ACAGCTCATG 
TACCAGACAG CTTTGATCGA GAGTGGATTC ATACTCACCG ACCCAAAAGA CTTTGCTGCC 
CGTATTTATA ACTCAGTCAA GAGCGGTCTG AACATCAGCC CTGATGCAGT AGCCGACGAG 
GAAATCGAGG CAGCAGAGGA ACCAGAGACC AGTGAGGCAA CTGAGACGAA ATCAGATGAC 
TTGGCTGGTG GTCTAAACAT TGAAGCCGAA CCCGTTGAGC AACAAGAAGA GAACACCAAG 
GACGAACTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHD ( NM_202877.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_974606.1 Arabidopsis thaliana Chaperone protein htpG family protein (SHD), mRNA.

Price & Availability↑

CloneID	OAb16134
Clone ID Related Accession (Same CDS sequence)	NM_202877.1
Accession Version	NM_202877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Chaperone protein htpG family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGAGGAAGA GGACGCTCGT GTCCGTTTTG TTCCTTTTCT CGCTTCTGTT TCTTCTTCCA 
GATCAAGGTA CAAAGTTACA CGCGAATGCA GAAGAGAGTT CCGATGATGT TACAGATCCA 
CCAAAAGTTG AGGAGAAAAT TGGTGGTCAC GGTGGTTTAT CGACGGATTC CGATGTAGTT 
CATAGAGAGT CTGAGTCGAT GTCAAAGAAG ACGCTTCGCA GTAACGCGGA GAAGTTTGAG 
TTCCAAGCTG AGGTTTCTCG GCTTATGGAC ATTATTATCA ACTCTCTATA CAGTAACAAG 
GACATTTTCT TAAGAGAGTT GATCTCCAAT GCTTCTGATG CCTTGGACAA AATTAGGTTC 
CTTGCACTCA CTGACAAAGA CGTTTTGGGT GAAGGTGACA CTGCTAAGCT TGAGATCCAG 
ATTAAGCTAG ACAAAGCCAA GAAGATTCTT TCTATTCGAG ACCGGGGAAT TGGTATGACT 
AAGGAAGACT TAATCAAGAA CCTTGGTACC ATAGCAAAAT CTGGAACGTC AGCTTTTGTT 
GAGAAAATGC AATCAAGTGG GGATTTGAAC CTTATTGGAC AATTTGGAGT TGGATTCTAC 
TCTGCCTATC TTGTTGCTGA CTACATTGAA GTCATTAGCA AGCACAATGA TGACAGCCAG 
TATGTATGGG AATCAAAGGC TAACGGTAAA TTTGCTGTTT CCGAGGATAC ATGGAATGAG 
CCTCTTGGAC GTGGAACCGA GATTAGATTA CATCTTAGGG ATGAAGCTGG CGAGTACCTA 
GAAGAAAGTA AACTTAAGGA ATTGGTGAAG AGATATTCTG AATTCATTAA CTTCCCTATC 
TCCCTCTGGG CTAGCAAAGA GGTTGAAACC GAGGTCCCAG TTGAGGAAGA TGAATCAGCT 
GATGAGGAAA CTGAAACCAC CTCTACCGAG GAAGAAAAGG AAGAAGATGC TGAGGAGGAA 
GATGGTGAGA AAAAGCAGAA AACCAAGAAG GTGAAAGAAA CAGTTTATGA ATGGGAGCTT 
CTGAATGATG TTAAGGCTAT ATGGCTGAGA AGTCCAAAGG AGGTGACAGA GGAAGAGTAC 
ACTAAATTCT ACCACTCTCT CTCAAAGGAT TTTACCGACG AGAAGCCAAT GGCTTGGAGT 
CACTTTAATG CTGAAGGTGA TGTTGAATTC AAGGCTGTGT TGTATGTTCC TCCGAAAGCT 
CCCCATGATC TATACGAGAG CTATTACAAC AGCAACAAGG CGAACTTGAA GCTATATGTC 
AGGAGGGTGT TCATCTCAGA TGAATTTGAT GAGCTTCTGC CTAAGTATTT GAGTTTCTTG 
AAGGGTCTTG TTGACTCTGA CACATTGCCT CTAAATGTAT CAAGAGAAAT GCTTCAACAA 
CACAGCAGCT TAAAGACAAT TAAGAAGAAG CTTATCCGAA AGGCGCTTGA TATGATCCGC 
AAGCTTGCTG AAGAAGATCC TGATGAAATC CATGATGATG AGAAAAAAGA TGTGGAGAAG 
TCTGGTGAGA ACGATGAGAA GAAGGGTCAA TACACAAAAT TCTGGAACGA GTTTGGCAAG 
TCTGTCAAAC TAGGTATCAT TGAGGATGCT GCTAACAGGA ACCGCTTGGC AAAACTTCTT 
CGTTTTGAGA CAACAAAGTC CGATGGAAAA TTGACTTCCC TGGATCAGTA CATTAAGAGA 
ATGAAAAAGA GCCAAAAGGA CATATTTTAC ATCACAGGAA GCAGCAAGGA ACAGCTAGAG 
AAATCTCCAT TCCTGGAGAG GCTCATCAAG AAGGGCTATG AGGTCATCTT CTTCACAGAC 
CCAGTTGATG AATACTTGAT GCAATACCTG ATGGATTACG AAGACAAAAA GTTCCAGAAT 
GTGTCCAAGG AAGGACTGAA GGTTGGGAAA GATTCAAAAG ACAAGGAGCT GAAGGAAGCA 
TTCAAGGAGC TAACAAAGTG GTGGAAAGGA AATCTCGCTA GCGAAAACGT AGACGATGTC 
AAAATCAGCA ACCGTTTGGC TGACACTCCA TGTGTAGTCG TAACATCCAA GTTTGGATGG 
AGTGCTAATA TGGAGAGGAT CATGCAGTCC CAAACTCTCT CGGATGCTAA TAAGCAAGCT 
TACATGCGCG GAAAGAGAGT CCTCGAGATC AACCCACGAC ACCCTATCAT CAAAGAACTC 
AAGGATAGAA TTGCAAGTGA CCCAGAGGAT GAGAGTGTGA AGGAAACAGC ACAGCTCATG 
TACCAGACAG CTTTGATCGA GAGTGGATTC ATACTCACCG ACCCAAAAGA CTTTGCTGCC 
CGTATTTATA ACTCAGTCAA GAGCGGTCTG AACATCAGCC CTGATGCAGT AGCCGACGAG 
GAAATCGAGG CAGCAGAGGA ACCAGAGACC AGTGAGGCAA CTGAGACGAA ATCAGATGAC 
TTGGCTGGTG GTCTAAACAT TGAAGCCGAA CCCGTTGAGC AACAAGAAGA GAACACCAAG 
GACGAACTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_974606.1
CDS	55..2526
Translation	MRKRTLVSVLFLFSLLFLLPDQGTKLHANAEESSDDVTDPPKVEEKIGGHGGLSTDSDVVHRESESMSKKTLRSNAEKFEFQAEVSRLMDIIINSLYSNKDIFLRELISNASDALDKIRFLALTDKDVLGEGDTAKLEIQIKLDKAKKILSIRDRGIGMTKEDLIKNLGTIAKSGTSAFVEKMQSSGDLNLIGQFGVGFYSAYLVADYIEVISKHNDDSQYVWESKANGKFAVSEDTWNEPLGRGTEIRLHLRDEAGEYLEESKLKELVKRYSEFINFPISLWASKEVETEVPVEEDESADEETETTSTEEEKEEDAEEEDGEKKQKTKKVKETVYEWELLNDVKAIWLRSPKEVTEEEYTKFYHSLSKDFTDEKPMAWSHFNAEGDVEFKAVLYVPPKAPHDLYESYYNSNKANLKLYVRRVFISDEFDELLPKYLSFLKGLVDSDTLPLNVSREMLQQHSSLKTIKKKLIRKALDMIRKLAEEDPDEIHDDEKKDVEKSGENDEKKGQYTKFWNEFGKSVKLGIIEDAANRNRLAKLLRFETTKSDGKLTSLDQYIKRMKKSQKDIFYITGSSKEQLEKSPFLERLIKKGYEVIFFTDPVDEYLMQYLMDYEDKKFQNVSKEGLKVGKDSKDKELKEAFKELTKWWKGNLASENVDDVKISNRLADTPCVVVTSKFGWSANMERIMQSQTLSDANKQAYMRGKRVLEINPRHPIIKELKDRIASDPEDESVKETAQLMYQTALIESGFILTDPKDFAARIYNSVKSGLNISPDAVADEEIEAAEEPETSEATETKSDDLAGGLNIEAEPVEQQEENTKDEL

Target ORF information:

RefSeq Version	NM_202877.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Chaperone protein htpG family protein (SHD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_202877.1

ORF Insert Sequence:

ATGAGGAAGA GGACGCTCGT GTCCGTTTTG TTCCTTTTCT CGCTTCTGTT TCTTCTTCCA 
GATCAAGGTA CAAAGTTACA CGCGAATGCA GAAGAGAGTT CCGATGATGT TACAGATCCA 
CCAAAAGTTG AGGAGAAAAT TGGTGGTCAC GGTGGTTTAT CGACGGATTC CGATGTAGTT 
CATAGAGAGT CTGAGTCGAT GTCAAAGAAG ACGCTTCGCA GTAACGCGGA GAAGTTTGAG 
TTCCAAGCTG AGGTTTCTCG GCTTATGGAC ATTATTATCA ACTCTCTATA CAGTAACAAG 
GACATTTTCT TAAGAGAGTT GATCTCCAAT GCTTCTGATG CCTTGGACAA AATTAGGTTC 
CTTGCACTCA CTGACAAAGA CGTTTTGGGT GAAGGTGACA CTGCTAAGCT TGAGATCCAG 
ATTAAGCTAG ACAAAGCCAA GAAGATTCTT TCTATTCGAG ACCGGGGAAT TGGTATGACT 
AAGGAAGACT TAATCAAGAA CCTTGGTACC ATAGCAAAAT CTGGAACGTC AGCTTTTGTT 
GAGAAAATGC AATCAAGTGG GGATTTGAAC CTTATTGGAC AATTTGGAGT TGGATTCTAC 
TCTGCCTATC TTGTTGCTGA CTACATTGAA GTCATTAGCA AGCACAATGA TGACAGCCAG 
TATGTATGGG AATCAAAGGC TAACGGTAAA TTTGCTGTTT CCGAGGATAC ATGGAATGAG 
CCTCTTGGAC GTGGAACCGA GATTAGATTA CATCTTAGGG ATGAAGCTGG CGAGTACCTA 
GAAGAAAGTA AACTTAAGGA ATTGGTGAAG AGATATTCTG AATTCATTAA CTTCCCTATC 
TCCCTCTGGG CTAGCAAAGA GGTTGAAACC GAGGTCCCAG TTGAGGAAGA TGAATCAGCT 
GATGAGGAAA CTGAAACCAC CTCTACCGAG GAAGAAAAGG AAGAAGATGC TGAGGAGGAA 
GATGGTGAGA AAAAGCAGAA AACCAAGAAG GTGAAAGAAA CAGTTTATGA ATGGGAGCTT 
CTGAATGATG TTAAGGCTAT ATGGCTGAGA AGTCCAAAGG AGGTGACAGA GGAAGAGTAC 
ACTAAATTCT ACCACTCTCT CTCAAAGGAT TTTACCGACG AGAAGCCAAT GGCTTGGAGT 
CACTTTAATG CTGAAGGTGA TGTTGAATTC AAGGCTGTGT TGTATGTTCC TCCGAAAGCT 
CCCCATGATC TATACGAGAG CTATTACAAC AGCAACAAGG CGAACTTGAA GCTATATGTC 
AGGAGGGTGT TCATCTCAGA TGAATTTGAT GAGCTTCTGC CTAAGTATTT GAGTTTCTTG 
AAGGGTCTTG TTGACTCTGA CACATTGCCT CTAAATGTAT CAAGAGAAAT GCTTCAACAA 
CACAGCAGCT TAAAGACAAT TAAGAAGAAG CTTATCCGAA AGGCGCTTGA TATGATCCGC 
AAGCTTGCTG AAGAAGATCC TGATGAAATC CATGATGATG AGAAAAAAGA TGTGGAGAAG 
TCTGGTGAGA ACGATGAGAA GAAGGGTCAA TACACAAAAT TCTGGAACGA GTTTGGCAAG 
TCTGTCAAAC TAGGTATCAT TGAGGATGCT GCTAACAGGA ACCGCTTGGC AAAACTTCTT 
CGTTTTGAGA CAACAAAGTC CGATGGAAAA TTGACTTCCC TGGATCAGTA CATTAAGAGA 
ATGAAAAAGA GCCAAAAGGA CATATTTTAC ATCACAGGAA GCAGCAAGGA ACAGCTAGAG 
AAATCTCCAT TCCTGGAGAG GCTCATCAAG AAGGGCTATG AGGTCATCTT CTTCACAGAC 
CCAGTTGATG AATACTTGAT GCAATACCTG ATGGATTACG AAGACAAAAA GTTCCAGAAT 
GTGTCCAAGG AAGGACTGAA GGTTGGGAAA GATTCAAAAG ACAAGGAGCT GAAGGAAGCA 
TTCAAGGAGC TAACAAAGTG GTGGAAAGGA AATCTCGCTA GCGAAAACGT AGACGATGTC 
AAAATCAGCA ACCGTTTGGC TGACACTCCA TGTGTAGTCG TAACATCCAA GTTTGGATGG 
AGTGCTAATA TGGAGAGGAT CATGCAGTCC CAAACTCTCT CGGATGCTAA TAAGCAAGCT 
TACATGCGCG GAAAGAGAGT CCTCGAGATC AACCCACGAC ACCCTATCAT CAAAGAACTC 
AAGGATAGAA TTGCAAGTGA CCCAGAGGAT GAGAGTGTGA AGGAAACAGC ACAGCTCATG 
TACCAGACAG CTTTGATCGA GAGTGGATTC ATACTCACCG ACCCAAAAGA CTTTGCTGCC 
CGTATTTATA ACTCAGTCAA GAGCGGTCTG AACATCAGCC CTGATGCAGT AGCCGACGAG 
GAAATCGAGG CAGCAGAGGA ACCAGAGACC AGTGAGGCAA CTGAGACGAA ATCAGATGAC 
TTGGCTGGTG GTCTAAACAT TGAAGCCGAA CCCGTTGAGC AACAAGAAGA GAACACCAAG 
GACGAACTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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SHD cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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