PRORP3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRORP3
Entrez Gene ID	828279
Full Name	proteinaceous RNase P 3
Synonyms	T8O5.110,T8O5_110,proteinaceous RNase P 3
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_118311.7	NP_193921.2	proteinaceous RNase P 3

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	PRORP1	NP_850186.1
Arabidopsis thaliana (thale cress)	PRORP2	NP_179256.3
Oryza sativa (rice)	Os02g0273800	NP_001046533.1
Arabidopsis thaliana (thale cress)	PRORP3	NP_193921.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The comparative biology of Escherichia coli RNase P and Arabidopsis thaliana PRORP isozymes has been reported.
Title: Protein-only RNase P function in Escherichia coli: viability, processing defects and differences between PRORP isoenzymes.

In contrast to bacterial RNase P, AtPRORP3 was found to be able to utilize the canonical and upstream cleavage site with similar efficiency (corresponding to reduced cleavage fidelity), and the two cleavage pathways appear less interdependent than in the bacterial RNA-based system.
Title: Analysis of the Cleavage Mechanism by Protein-Only RNase P Using Precursor tRNA Substrates with Modifications at the Cleavage Site.

Substrate recognition and cleavage-site selection by PRORP3 has been described.
Title: Substrate recognition and cleavage-site selection by a single-subunit protein-only RNase P.

The study presents the first kinetic analysis of PRORP1, PRORP2 and PRORP3.
Title: tRNA processing by protein-only versus RNA-based RNase P: kinetic analysis reveals mechanistic differences.

The following PRORP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRORP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb15915	NM_118311.7 Latest version!	Arabidopsis thaliana proteinaceous RNase P 3 (PRORP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRORP3 ( NM_118311.7 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_193921.2 Arabidopsis thaliana proteinaceous RNase P 3 (PRORP3), mRNA.

Price & Availability↑

CloneID	OAb15915
Clone ID Related Accession (Same CDS sequence)	NM_118311.7
Accession Version	NM_118311.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	proteinaceous RNase P 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_118311.6.

ATGAAATTGA AAAAACCTTC ATTGCCTTCT TCATTGCTCT GCGCCGTCCC GCCTTGTCTC 
TCTCAAATTC GCTTACTCAT CCCTCGCAGG GTAAGGGTTT CATCATCAAC TTTCGCCAAT 
GCAAAGCTTG TAACTTTGAG AAACCACACT GTTAATTTGC ACATATATTA CTGTTCCATG 
GCTGGTACTG ATAACCGCCG CTCTCGCCAC GACGACGAAA GCCCTAAAAA TCCCAACAAG 
AAGAAGAAAG GAAACCGTAA TCCAGAAAAG AGTCTCCTTA TCAATCTTCA TTCATGTTCC 
AAGAGAAAGG ATCTCTCAGC TGCATTGGCT CTTTATGATG CAGCTATCAC TTCCAGTGAC 
ATTCGCCTCA ACCAGCAACA CTTCCAATCC CTTCTTTACC TCTGCTCCGC GTTCATTAGT 
GACCCATCTC TTCAAACCGT TGCCATTGAC CGTGGCTTTC AAATTTTTGA CCGTATGGTT 
AGTTCTGGGA TAAGTCCTAA TGAATCATCT GTCACTGCAG TTGCACGACT AGCTGCTGCC 
AAGGGTGATG GAGACTATGC TTTCAAGCTT GTTAAAGACC TTGTTGCTGT TGGTGGTGTA 
TCGGTCCCTC GTCTGAGAAC TTATGCTCCG GCTTTGCTCT GTTTCTGTGA TACTTTGGAG 
GCTGAAAAGG GTTATGAAGT GGAAGATCAC ATGGATGCTT CAGGTATTGT GTTGGAGGAG 
GCAGAGATCT CGGCTTTGCT AAAGGTAAGC GCTGCCACAG GCCGAGAAAA CAAGGTTTAT 
AGATATCTGC AGAAGTTAAG AGAATGTGTT GGCTGCGTTA GTGAAGAAAC TTCAAAAGCA 
ATCGAGGAAT GGTTTTATGG AGTGAAAGCT AGCGAAGTTA GTGATAATGG GATTGGTTCT 
GATATTGAGC TGCTTAGAGC AGCTGTTTTG AAAAATGGGG GTGGGTGGCA TGGTCTTGGG 
TGGGTTGGAG AAGGCAAATG GATTGTGAAG AAAGGTAATG TTAGCTCAGC CGGGAAATGT 
TTGAGCTGTG ATGAGCATTT GGCTTGTGTT GATACCAATG AGGTAGAGAC TGAAGATTTT 
GTGAATTCTT TGGTGACTTT GGCTATGGAG AGGAAGGCAA AGATGAATTC ATGCGAGCCT 
ATGGCGGATT TCAGTGAGTT TCAGGAGTGG CTTGAAAAGC ATGGAGATTA CGAAGCTATA 
CTAGATGGAG CAAATATTGG GCTCTACCAA CAAAATTTTG CAGATGGTGG TTTCAGTCTA 
CCACAGCTTG AAGCTGTAGT GAAGGAACTT TACAATAAAA GTGGTAGCAA AAAACAGCCG 
CTAATTCTCT TGCACAAGAA ACGGGTCAAT GCGCTTTTAG AAAACCCGAA TCACCGGAAT 
CTTGTGGAAG AATGGATTAA TAACAATGTC CTGTATGCGA CTCCACCAGG TTCCAATGAT 
GATTGGTATT GGCTCTACGC TGCTGCTAAA CTCAAGTGTT TACTTGTGAC TAATGATGAG 
ATGAGAGATC ACATTTTTGA ACTATTAAGT AACAGTTTCT TCCAAAAATG GAAAGAAAGA 
CATCAAGTTC GGTTTACCTT TGTGAAAGGC TGTCTCAAGC TTGAAATGCC ACCTCCTTTT 
TCAGTTGTGA TCCAGGAATC GGAGAAAGGA TCATGGCACG TTCCAATCAC GAGTCAAGAC 
AAGGAGGAAT CTTTAAGAAG TTGGATGTGC ATTACAAGGC AGAGTTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_193921.2
CDS	73..1803
Translation	MKLKKPSLPSSLLCAVPPCLSQIRLLIPRRVRVSSSTFANAKLVTLRNHTVNLHIYYCSMAGTDNRRSRHDDESPKNPNKKKKGNRNPEKSLLINLHSCSKRKDLSAALALYDAAITSSDIRLNQQHFQSLLYLCSAFISDPSLQTVAIDRGFQIFDRMVSSGISPNESSVTAVARLAAAKGDGDYAFKLVKDLVAVGGVSVPRLRTYAPALLCFCDTLEAEKGYEVEDHMDASGIVLEEAEISALLKVSAATGRENKVYRYLQKLRECVGCVSEETSKAIEEWFYGVKASEVSDNGIGSDIELLRAAVLKNGGGWHGLGWVGEGKWIVKKGNVSSAGKCLSCDEHLACVDTNEVETEDFVNSLVTLAMERKAKMNSCEPMADFSEFQEWLEKHGDYEAILDGANIGLYQQNFADGGFSLPQLEAVVKELYNKSGSKKQPLILLHKKRVNALLENPNHRNLVEEWINNNVLYATPPGSNDDWYWLYAAAKLKCLLVTNDEMRDHIFELLSNSFFQKWKERHQVRFTFVKGCLKLEMPPPFSVVIQESEKGSWHVPITSQDKEESLRSWMCITRQSS

Target ORF information:

RefSeq Version	NM_118311.7
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana proteinaceous RNase P 3 (PRORP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118311.7

ORF Insert Sequence:

ATGAAATTGA AAAAACCTTC ATTGCCTTCT TCATTGCTCT GCGCCGTCCC GCCTTGTCTC 
TCTCAAATTC GCTTACTCAT CCCTCGCAGG GTAAGGGTTT CATCATCAAC TTTCGCCAAT 
GCAAAGCTTG TAACTTTGAG AAACCACACT GTTAATTTGC ACATATATTA CTGTTCCATG 
GCTGGTACTG ATAACCGCCG CTCTCGCCAC GACGACGAAA GCCCTAAAAA TCCCAACAAG 
AAGAAGAAAG GAAACCGTAA TCCAGAAAAG AGTCTCCTTA TCAATCTTCA TTCATGTTCC 
AAGAGAAAGG ATCTCTCAGC TGCATTGGCT CTTTATGATG CAGCTATCAC TTCCAGTGAC 
ATTCGCCTCA ACCAGCAACA CTTCCAATCC CTTCTTTACC TCTGCTCCGC GTTCATTAGT 
GACCCATCTC TTCAAACCGT TGCCATTGAC CGTGGCTTTC AAATTTTTGA CCGTATGGTT 
AGTTCTGGGA TAAGTCCTAA TGAATCATCT GTCACTGCAG TTGCACGACT AGCTGCTGCC 
AAGGGTGATG GAGACTATGC TTTCAAGCTT GTTAAAGACC TTGTTGCTGT TGGTGGTGTA 
TCGGTCCCTC GTCTGAGAAC TTATGCTCCG GCTTTGCTCT GTTTCTGTGA TACTTTGGAG 
GCTGAAAAGG GTTATGAAGT GGAAGATCAC ATGGATGCTT CAGGTATTGT GTTGGAGGAG 
GCAGAGATCT CGGCTTTGCT AAAGGTAAGC GCTGCCACAG GCCGAGAAAA CAAGGTTTAT 
AGATATCTGC AGAAGTTAAG AGAATGTGTT GGCTGCGTTA GTGAAGAAAC TTCAAAAGCA 
ATCGAGGAAT GGTTTTATGG AGTGAAAGCT AGCGAAGTTA GTGATAATGG GATTGGTTCT 
GATATTGAGC TGCTTAGAGC AGCTGTTTTG AAAAATGGGG GTGGGTGGCA TGGTCTTGGG 
TGGGTTGGAG AAGGCAAATG GATTGTGAAG AAAGGTAATG TTAGCTCAGC CGGGAAATGT 
TTGAGCTGTG ATGAGCATTT GGCTTGTGTT GATACCAATG AGGTAGAGAC TGAAGATTTT 
GTGAATTCTT TGGTGACTTT GGCTATGGAG AGGAAGGCAA AGATGAATTC ATGCGAGCCT 
ATGGCGGATT TCAGTGAGTT TCAGGAGTGG CTTGAAAAGC ATGGAGATTA CGAAGCTATA 
CTAGATGGAG CAAATATTGG GCTCTACCAA CAAAATTTTG CAGATGGTGG TTTCAGTCTA 
CCACAGCTTG AAGCTGTAGT GAAGGAACTT TACAATAAAA GTGGTAGCAA AAAACAGCCG 
CTAATTCTCT TGCACAAGAA ACGGGTCAAT GCGCTTTTAG AAAACCCGAA TCACCGGAAT 
CTTGTGGAAG AATGGATTAA TAACAATGTC CTGTATGCGA CTCCACCAGG TTCCAATGAT 
GATTGGTATT GGCTCTACGC TGCTGCTAAA CTCAAGTGTT TACTTGTGAC TAATGATGAG 
ATGAGAGATC ACATTTTTGA ACTATTAAGT AACAGTTTCT TCCAAAAATG GAAAGAAAGA 
CATCAAGTTC GGTTTACCTT TGTGAAAGGC TGTCTCAAGC TTGAAATGCC ACCTCCTTTT 
TCAGTTGTGA TCCAGGAATC GGAGAAAGGA TCATGGCACG TTCCAATCAC GAGTCAAGAC 
AAGGAGGAAT CTTTAAGAAG TTGGATGTGC ATTACAAGGC AGAGTTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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PRORP3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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