TOC159 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOC159
Entrez Gene ID	827934
Full Name	translocon at the outer envelope membrane of chloroplasts 159
Synonyms	ATTOC159,PLASTID PROTEIN IMPORT 2,PPI2,T10P11.19,TOC160,TOC86,TRANSLOCON AT THE OUTER ENVELOPE MEMBRANE OF CHLOROPLASTS 160,TRANSLOCON AT THE OUTER ENVELOPE MEMBRANE OF CHLOROPLASTS 86,translocon at the outer envelope membrane of chloroplasts 159
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001340383.1	NP_001319848.1	translocon at the outer envelope membrane of chloroplasts 159
NM_116485.4	NP_567242.2	translocon at the outer envelope membrane of chloroplasts 159
NM_001340385.1	NP_001328635.1	translocon at the outer envelope membrane of chloroplasts 159
NM_001340384.1	NP_001328636.1	translocon at the outer envelope membrane of chloroplasts 159

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

atToc132 can serve as a receptor by directly binding to chloroplast preproteins but also proposes the existence of subsets of preproteins with different but overlapping affinities for more than one member of the Toc159 receptor family.
Title: A split-ubiquitin yeast two-hybrid screen to examine the substrate specificity of atToc159 and atToc132, two Arabidopsis chloroplast preprotein import receptors.

these data suggest that the Toc159 receptor is required in the import of stearoyl-ACP desaturase and MGD1 synthase into the chloroplasts.
Title: A mutant of the Arabidopsis thaliana TOC159 gene accumulates reduced levels of linolenic acid and monogalactosyldiacylglycerol.

the contribution of the import receptor Toc159 to the assembly of the chloroplast proteome
Title: Plastid proteome assembly without Toc159: photosynthetic protein import and accumulation of N-acetylated plastid precursor proteins.

This review discusses TOC33 and TOC159, the major protein components of the translocon at the outer envelope membrane of chloroplasts (TOC) complex.
Title: Dimerization of TOC receptor GTPases and its implementation for the control of protein import into chloroplasts.

Study show that the overexpression of Toc90 partially complements the albino knockout of Toc159 and restores photoautotrophic growth.
Title: The chloroplast import receptor Toc90 partially restores the accumulation of Toc159 client proteins in the Arabidopsis thaliana ppi2 mutant.

The following TOC159 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOC159 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb15601	NM_001340385.1 Latest version!	Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 159 (TOC159), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAb15601	NM_116485.4 Latest version!	Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 159 (TOC159), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAb15601	NM_001340383.1 Latest version!	Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 159 (TOC159), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAb15601	NM_001340384.1 Latest version!	Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 159 (TOC159), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOC159 ( NM_001340383.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001319848.1 Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 159 (TOC159), mRNA.

Price & Availability↑

CloneID	OAb15601
Clone ID Related Accession (Same CDS sequence)	NM_001340383.1 , NM_116485.4 , NM_001340385.1 , NM_001340384.1
Accession Version	NM_001340383.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	translocon at the outer envelope membrane of chloroplasts 159
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGACTCAA AGTCGGTTAC TCCAGAACCA ACCAACCCCT TCTACGCTTC TTCGGGGCAA 
TCAGGAAAAA CCTATGCTTC TGTTGTCGCC GCCGCTGCTG CTGCAGCCGC CGATAAGGAG 
GATGGTGGTG CTGTGAGTAG TGCCAAGGAG TTGGATTCCT CATCGGAGGC TGTGTCTGGT 
AATTCGGATA AGGTTGGAGC TGATGATTTA TCTGACTCCG AGAAGGAGAA GCCGAATTTG 
GTGGGTGATG GGAAGGTTTC CGACGAGGTG GATGGTTCTT TAAAGGAGGA TTCTACTACT 
CCTGAGGCTA CTCCGAAGCC TGAGGTGGTT TCTGGTGAGA CAATTGGTGT AGATGATGTT 
TCATCGTTAT CTCCGAAGCC GGAGGCTGTT TCTGATGGTG TAGGGGTTGT GGAGGAGAAT 
AAGAAGGTTA AGGAGGACGT GGAGGATATT AAAGACGATG GTGAGAGTAA GATTGAAAAT 
GGGAGTGTTG ATGTTGATGT GAAACAGGCT TCCACAGATG GGGAGAGTGA GAGTAAAGTG 
AAGGATGTGG AAGAAGAAGA TGTTGGAACA AAAAAAGATG ATGAGGGGGA GAGTGAGTTG 
GGTGGAAAAG TTGATGTTGA CGATAAAAGT GATAATGTTA TTGAAGAAGA GGGTGTGGAG 
TTAACTGATA AAGGAGACGT CATTGTGAAC TCGTCCCCTG TAGAATCAGT GCATGTTGAT 
GTGGCAAAAC CAGGGGTGGT TGTTGTAGGA GACGCAGAGG GAAGTGAGGA GTTGAAGATT 
AATGCTGATG CTGAAACTCT TGAAGTGGCT AATAAGTTTG ATCAAATTGG GGATGATGAT 
AGTGGTGAAT TTGAACCTGT GTCTGACAAG GCCATTGAGG AGGTAGAAGA AAAATTTACT 
AGCGAATCAG ACTCTATTGC AGACTCCTCT AAGTTAGAAT CTGTGGATAC CAGTGCTGTA 
GAGCCAGAGG TTGTAGCTGC CGAGAGTGGG AGTGAACCAA AGGATGTGGA AAAAGCCAAC 
GGATTGGAGA AGGGTATGAC TTATGCTGAA GTAATCAAGG CAGCTAGTGC AGTAGCTGAC 
AATGGAACCA AAGAGGAGGA GAGTGTGCTC GGTGGCATAG TCGATGATGC AGAAGAAGGT 
GTTAAGTTAA ACAACAAAGG AGACTTTGTG GTGGACTCAT CTGCTATTGA AGCTGTGAAT 
GTAGATGTGG CAAAGCCAGG GGTTGTTGTT GTTGGAGACG TAGAGGTGAG TGAGGTGCTG 
GAAACTGATG GCAACATCCC TGATGTGCAT AATAAGTTTG ATCCAATTGG CCAAGGTGAA 
GGTGGTGAAG TTGAACTTGA ATCCGATAAA GCCACAGAGG AGGGAGGAGG AAAGTTGGTT 
AGTGAAGGAG ACTCTATGGT TGACTCCTCT GTGGTGGACT CTGTTGATGC TGATATCAAT 
GTTGCAGAGC CAGGGGTTGT GGTTGTCGGG GCGGCTAAGG AGGCTGTAAT TAAGGAAGAT 
GATAAAGATG ATGAGGTTGA CAAGACCATC TCAAATATTG AGGAACCTGA TGACCTGACT 
GCTGCATATG ATGGAAATTT TGAATTGGCT GTTAAGGAAA TATCAGAGGC TGCCAAAGTA 
GAGCCAGACG AACCAAAAGT AGGTGTGGAA GTAGAAGAAT TGCCTGTTTC TGAAAGTTTA 
AAAGTGGGTT CAGTTGATGC AGAAGAGGAT TCAATTCCTG CAGCTGAGTC ACAGTTTGAG 
GTTCGGAAAG TTGTCGAAGG GGACAGTGCT GAAGAGGATG AAAACAAGCT CCCAGTCGAA 
GACATTGTTT CTTCTCGTGA GTTTAGCTTT GGAGGCAAGG AAGTGGACCA GGAACCTTCT 
GGCGAAGGTG TTACCAGGGT TGATGGGTCT GAAAGTGAAG AAGAAACTGA AGAGATGATA 
TTTGGGAGTT CTGAAGCTGC AAAACAGTTC TTAGCAGAGT TGGAAAAGGC TTCTTCTGGT 
ATAGAGGCAC ATTCTGATGA AGCAAACATT TCTAACAATA TGTCAGATAG GATTGATGGC 
CAGATTGTCA CCGATTCTGA CGAGGATGTA GACACAGAGG ATGAAGGTGA GGAGAAAATG 
TTTGATACTG CAGCTTTGGC TGCGCTTTTG AAGGCAGCCA CTGGTGGTGG AAGTTCAGAA 
GGTGGCAATT TTACCATAAC ATCTCAGGAT GGCACGAAGC TTTTCTCTAT GGATCGACCT 
GCTGGTTTGA GTTCATCGTT AAGGCCCTTG AAGCCTGCAG CAGCTCCACG TGCAAACCGT 
TCCAACATCT TTTCCAATTC TAATGTCACA ATGGCAGATG AGACTGAGAT TAACTTGAGC 
GAGGAAGAGA AACAGAAATT AGAAAAGTTG CAAAGCCTCC GTGTGAAGTT TTTGCGGCTT 
TTGCAAAGGT TGGGTCATTC GGCAGAAGAT TCAATTGCAG CGCAGGTTCT TTACCGTCTT 
GCACTACTTG CTGGGAGACA AGCAGGGCAG TTATTCAGTC TTGATGCTGC AAAGAAGAAG 
GCTGTGGAGT CTGAGGCTGA GGGCAACGAA GAATTGATCT TCTCCCTAAA CATACTGGTC 
CTTGGAAAAG CCGGGGTGGG AAAAAGTGCT ACTATAAATT CCATTTTGGG AAACCAGATT 
GCGTCCATTG ATGCTTTTGG GCTCTCAACC ACTTCGGTTA GAGAAATTTC TGGAACGGTG 
AATGGTGTCA AGATTACCTT TATTGATACT CCGGGTTTGA AGTCTGCTGC GATGGATCAA 
AGTACAAATG CAAAAATGTT GTCCTCTGTA AAGAAGGTAA TGAAGAAGTG TCCCCCTGAT 
ATTGTACTAT ATGTAGATCG TCTTGACACC CAGACCAGGG ACTTGAACAA TTTGCCCTTG 
CTAAGGACGA TTACTGCTTC TCTTGGTACA TCCATATGGA AAAACGCTAT AGTGACATTG 
ACCCATGCGG CTTCTGCCCC TCCTGATGGC CCATCTGGCA CCCCATTGAG CTATGATGTG 
TTTGTAGCGC AGTGCTCACA TATTGTGCAA CAGTCTATAG GACAGGCTGT TGGAGATCTA 
CGCTTGATGA ATCCAAGTCT GATGAATCCA GTTTCACTTG TTGAGAATCA CCCCTTGTGT 
AGGAAGAACC GGGAGGGAGT GAAGGTTCTC CCGAATGGCC AAACTTGGAG ATCTCAGCTG 
TTGCTATTGT GTTACTCCCT GAAGGTTTTG TCAGAAACAA ATTCTCTATT GAGGCCTCAA 
GAACCATTGG ACCATCGTAA AGTATTTGGT TTCCGAGTTA GATCCCCGCC TCTCCCTTAC 
TTGTTGTCTT GGCTGTTGCA GTCCCGTGCA CATCCTAAGC TCCCTGGAGA CCAAGGTGGT 
GATAGTGTTG ACTCTGATAT CGAAATTGAT GATGTGTCTG ATTCTGAGCA GGAAGATGGG 
GAAGATGATG AGTATGACCA GCTGCCACCA TTTAAGCCTC TTCGAAAAAC CCAACTTGCA 
AAGCTCTCAA ATGAACAGAG AAAGGCATAT TTCGAGGAGT ATGATTACCG TGTAAAGCTC 
CTGCAGAAGA AGCAATGGAG AGAGGAATTA AAGAGGATGA AAGAAATGAA GAAAAACGGG 
AAGAAGCTGG GTGAAAGTGA GTTTGGTTAT CCGGGAGAAG AAGATGATCC AGAAAACGGG 
GCTCCAGCTG CAGTGCCAGT TCCATTACCC GATATGGTTT TGCCACCTTC ATTTGATAGT 
GATAACTCAG CTTACCGATA CCGATATCTG GAACCCACTT CACAACTCCT AACCAGGCCA 
GTGTTGGATA CCCATGGTTG GGACCATGAC TGTGGATATG ACGGCGTCAA TGCAGAACAC 
AGTCTTGCTC TAGCTAGCCG GTTCCCTGCC ACAGCTACTG TCCAAGTCAC CAAGGACAAG 
AAAGAGTTCA ACATTCATCT GGACTCCTCT GTGTCTGCTA AGCACGGGGA GAATGGATCC 
ACCATGGCAG GGTTCGATAT TCAGAATGTA GGCAAGCAGC TGGCATATGT GGTCAGAGGA 
GAAACCAAAT TCAAGAATTT GAGGAAGAAC AAGACAACTG TTGGAGGGTC AGTGACATTC 
TTGGGAGAGA ACATCGCCAC TGGGGTCAAA CTCGAGGACC AAATAGCACT GGGGAAAAGG 
TTGGTGCTTG TGGGCAGCAC TGGGACAATG CGATCACAGG GAGATTCGGC CTATGGTGCG 
AACCTCGAGG TCAGGCTTAG GGAAGCTGAT TTCCCAATTG GACAGGACCA ATCTTCTTTT 
GGGCTGTCTC TGGTAAAGTG GAGAGGCGAT TTAGCCCTTG GAGCCAATCT CCAATCTCAA 
GTCTCTGTTG GAAGGAACTC AAAGATTGCG CTTCGTGCAG GACTTAACAA CAAGATGAGC 
GGACAGATCA CAGTCAGAAC CAGCAGCTCG GATCAGTTGC AAATCGCTCT CACAGCCATT 
CTTCCAATTG CCATGTCCAT CTACAAGAGC ATTCGACCCG AAGCGACGAA CGACAAGTAC 
AGCATGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001319848.1
CDS	338..4849
Translation	MDSKSVTPEPTNPFYASSGQSGKTYASVVAAAAAAAADKEDGGAVSSAKELDSSSEAVSGNSDKVGADDLSDSEKEKPNLVGDGKVSDEVDGSLKEDSTTPEATPKPEVVSGETIGVDDVSSLSPKPEAVSDGVGVVEENKKVKEDVEDIKDDGESKIENGSVDVDVKQASTDGESESKVKDVEEEDVGTKKDDEGESELGGKVDVDDKSDNVIEEEGVELTDKGDVIVNSSPVESVHVDVAKPGVVVVGDAEGSEELKINADAETLEVANKFDQIGDDDSGEFEPVSDKAIEEVEEKFTSESDSIADSSKLESVDTSAVEPEVVAAESGSEPKDVEKANGLEKGMTYAEVIKAASAVADNGTKEEESVLGGIVDDAEEGVKLNNKGDFVVDSSAIEAVNVDVAKPGVVVVGDVEVSEVLETDGNIPDVHNKFDPIGQGEGGEVELESDKATEEGGGKLVSEGDSMVDSSVVDSVDADINVAEPGVVVVGAAKEAVIKEDDKDDEVDKTISNIEEPDDLTAAYDGNFELAVKEISEAAKVEPDEPKVGVEVEELPVSESLKVGSVDAEEDSIPAAESQFEVRKVVEGDSAEEDENKLPVEDIVSSREFSFGGKEVDQEPSGEGVTRVDGSESEEETEEMIFGSSEAAKQFLAELEKASSGIEAHSDEANISNNMSDRIDGQIVTDSDEDVDTEDEGEEKMFDTAALAALLKAATGGGSSEGGNFTITSQDGTKLFSMDRPAGLSSSLRPLKPAAAPRANRSNIFSNSNVTMADETEINLSEEEKQKLEKLQSLRVKFLRLLQRLGHSAEDSIAAQVLYRLALLAGRQAGQLFSLDAAKKKAVESEAEGNEELIFSLNILVLGKAGVGKSATINSILGNQIASIDAFGLSTTSVREISGTVNGVKITFIDTPGLKSAAMDQSTNAKMLSSVKKVMKKCPPDIVLYVDRLDTQTRDLNNLPLLRTITASLGTSIWKNAIVTLTHAASAPPDGPSGTPLSYDVFVAQCSHIVQQSIGQAVGDLRLMNPSLMNPVSLVENHPLCRKNREGVKVLPNGQTWRSQLLLLCYSLKVLSETNSLLRPQEPLDHRKVFGFRVRSPPLPYLLSWLLQSRAHPKLPGDQGGDSVDSDIEIDDVSDSEQEDGEDDEYDQLPPFKPLRKTQLAKLSNEQRKAYFEEYDYRVKLLQKKQWREELKRMKEMKKNGKKLGESEFGYPGEEDDPENGAPAAVPVPLPDMVLPPSFDSDNSAYRYRYLEPTSQLLTRPVLDTHGWDHDCGYDGVNAEHSLALASRFPATATVQVTKDKKEFNIHLDSSVSAKHGENGSTMAGFDIQNVGKQLAYVVRGETKFKNLRKNKTTVGGSVTFLGENIATGVKLEDQIALGKRLVLVGSTGTMRSQGDSAYGANLEVRLREADFPIGQDQSSFGLSLVKWRGDLALGANLQSQVSVGRNSKIALRAGLNNKMSGQITVRTSSSDQLQIALTAILPIAMSIYKSIRPEATNDKYSMY

Target ORF information:

RefSeq Version	NM_001340383.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 159 (TOC159), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001340383.1

ORF Insert Sequence:

ATGGACTCAA AGTCGGTTAC TCCAGAACCA ACCAACCCCT TCTACGCTTC TTCGGGGCAA 
TCAGGAAAAA CCTATGCTTC TGTTGTCGCC GCCGCTGCTG CTGCAGCCGC CGATAAGGAG 
GATGGTGGTG CTGTGAGTAG TGCCAAGGAG TTGGATTCCT CATCGGAGGC TGTGTCTGGT 
AATTCGGATA AGGTTGGAGC TGATGATTTA TCTGACTCCG AGAAGGAGAA GCCGAATTTG 
GTGGGTGATG GGAAGGTTTC CGACGAGGTG GATGGTTCTT TAAAGGAGGA TTCTACTACT 
CCTGAGGCTA CTCCGAAGCC TGAGGTGGTT TCTGGTGAGA CAATTGGTGT AGATGATGTT 
TCATCGTTAT CTCCGAAGCC GGAGGCTGTT TCTGATGGTG TAGGGGTTGT GGAGGAGAAT 
AAGAAGGTTA AGGAGGACGT GGAGGATATT AAAGACGATG GTGAGAGTAA GATTGAAAAT 
GGGAGTGTTG ATGTTGATGT GAAACAGGCT TCCACAGATG GGGAGAGTGA GAGTAAAGTG 
AAGGATGTGG AAGAAGAAGA TGTTGGAACA AAAAAAGATG ATGAGGGGGA GAGTGAGTTG 
GGTGGAAAAG TTGATGTTGA CGATAAAAGT GATAATGTTA TTGAAGAAGA GGGTGTGGAG 
TTAACTGATA AAGGAGACGT CATTGTGAAC TCGTCCCCTG TAGAATCAGT GCATGTTGAT 
GTGGCAAAAC CAGGGGTGGT TGTTGTAGGA GACGCAGAGG GAAGTGAGGA GTTGAAGATT 
AATGCTGATG CTGAAACTCT TGAAGTGGCT AATAAGTTTG ATCAAATTGG GGATGATGAT 
AGTGGTGAAT TTGAACCTGT GTCTGACAAG GCCATTGAGG AGGTAGAAGA AAAATTTACT 
AGCGAATCAG ACTCTATTGC AGACTCCTCT AAGTTAGAAT CTGTGGATAC CAGTGCTGTA 
GAGCCAGAGG TTGTAGCTGC CGAGAGTGGG AGTGAACCAA AGGATGTGGA AAAAGCCAAC 
GGATTGGAGA AGGGTATGAC TTATGCTGAA GTAATCAAGG CAGCTAGTGC AGTAGCTGAC 
AATGGAACCA AAGAGGAGGA GAGTGTGCTC GGTGGCATAG TCGATGATGC AGAAGAAGGT 
GTTAAGTTAA ACAACAAAGG AGACTTTGTG GTGGACTCAT CTGCTATTGA AGCTGTGAAT 
GTAGATGTGG CAAAGCCAGG GGTTGTTGTT GTTGGAGACG TAGAGGTGAG TGAGGTGCTG 
GAAACTGATG GCAACATCCC TGATGTGCAT AATAAGTTTG ATCCAATTGG CCAAGGTGAA 
GGTGGTGAAG TTGAACTTGA ATCCGATAAA GCCACAGAGG AGGGAGGAGG AAAGTTGGTT 
AGTGAAGGAG ACTCTATGGT TGACTCCTCT GTGGTGGACT CTGTTGATGC TGATATCAAT 
GTTGCAGAGC CAGGGGTTGT GGTTGTCGGG GCGGCTAAGG AGGCTGTAAT TAAGGAAGAT 
GATAAAGATG ATGAGGTTGA CAAGACCATC TCAAATATTG AGGAACCTGA TGACCTGACT 
GCTGCATATG ATGGAAATTT TGAATTGGCT GTTAAGGAAA TATCAGAGGC TGCCAAAGTA 
GAGCCAGACG AACCAAAAGT AGGTGTGGAA GTAGAAGAAT TGCCTGTTTC TGAAAGTTTA 
AAAGTGGGTT CAGTTGATGC AGAAGAGGAT TCAATTCCTG CAGCTGAGTC ACAGTTTGAG 
GTTCGGAAAG TTGTCGAAGG GGACAGTGCT GAAGAGGATG AAAACAAGCT CCCAGTCGAA 
GACATTGTTT CTTCTCGTGA GTTTAGCTTT GGAGGCAAGG AAGTGGACCA GGAACCTTCT 
GGCGAAGGTG TTACCAGGGT TGATGGGTCT GAAAGTGAAG AAGAAACTGA AGAGATGATA 
TTTGGGAGTT CTGAAGCTGC AAAACAGTTC TTAGCAGAGT TGGAAAAGGC TTCTTCTGGT 
ATAGAGGCAC ATTCTGATGA AGCAAACATT TCTAACAATA TGTCAGATAG GATTGATGGC 
CAGATTGTCA CCGATTCTGA CGAGGATGTA GACACAGAGG ATGAAGGTGA GGAGAAAATG 
TTTGATACTG CAGCTTTGGC TGCGCTTTTG AAGGCAGCCA CTGGTGGTGG AAGTTCAGAA 
GGTGGCAATT TTACCATAAC ATCTCAGGAT GGCACGAAGC TTTTCTCTAT GGATCGACCT 
GCTGGTTTGA GTTCATCGTT AAGGCCCTTG AAGCCTGCAG CAGCTCCACG TGCAAACCGT 
TCCAACATCT TTTCCAATTC TAATGTCACA ATGGCAGATG AGACTGAGAT TAACTTGAGC 
GAGGAAGAGA AACAGAAATT AGAAAAGTTG CAAAGCCTCC GTGTGAAGTT TTTGCGGCTT 
TTGCAAAGGT TGGGTCATTC GGCAGAAGAT TCAATTGCAG CGCAGGTTCT TTACCGTCTT 
GCACTACTTG CTGGGAGACA AGCAGGGCAG TTATTCAGTC TTGATGCTGC AAAGAAGAAG 
GCTGTGGAGT CTGAGGCTGA GGGCAACGAA GAATTGATCT TCTCCCTAAA CATACTGGTC 
CTTGGAAAAG CCGGGGTGGG AAAAAGTGCT ACTATAAATT CCATTTTGGG AAACCAGATT 
GCGTCCATTG ATGCTTTTGG GCTCTCAACC ACTTCGGTTA GAGAAATTTC TGGAACGGTG 
AATGGTGTCA AGATTACCTT TATTGATACT CCGGGTTTGA AGTCTGCTGC GATGGATCAA 
AGTACAAATG CAAAAATGTT GTCCTCTGTA AAGAAGGTAA TGAAGAAGTG TCCCCCTGAT 
ATTGTACTAT ATGTAGATCG TCTTGACACC CAGACCAGGG ACTTGAACAA TTTGCCCTTG 
CTAAGGACGA TTACTGCTTC TCTTGGTACA TCCATATGGA AAAACGCTAT AGTGACATTG 
ACCCATGCGG CTTCTGCCCC TCCTGATGGC CCATCTGGCA CCCCATTGAG CTATGATGTG 
TTTGTAGCGC AGTGCTCACA TATTGTGCAA CAGTCTATAG GACAGGCTGT TGGAGATCTA 
CGCTTGATGA ATCCAAGTCT GATGAATCCA GTTTCACTTG TTGAGAATCA CCCCTTGTGT 
AGGAAGAACC GGGAGGGAGT GAAGGTTCTC CCGAATGGCC AAACTTGGAG ATCTCAGCTG 
TTGCTATTGT GTTACTCCCT GAAGGTTTTG TCAGAAACAA ATTCTCTATT GAGGCCTCAA 
GAACCATTGG ACCATCGTAA AGTATTTGGT TTCCGAGTTA GATCCCCGCC TCTCCCTTAC 
TTGTTGTCTT GGCTGTTGCA GTCCCGTGCA CATCCTAAGC TCCCTGGAGA CCAAGGTGGT 
GATAGTGTTG ACTCTGATAT CGAAATTGAT GATGTGTCTG ATTCTGAGCA GGAAGATGGG 
GAAGATGATG AGTATGACCA GCTGCCACCA TTTAAGCCTC TTCGAAAAAC CCAACTTGCA 
AAGCTCTCAA ATGAACAGAG AAAGGCATAT TTCGAGGAGT ATGATTACCG TGTAAAGCTC 
CTGCAGAAGA AGCAATGGAG AGAGGAATTA AAGAGGATGA AAGAAATGAA GAAAAACGGG 
AAGAAGCTGG GTGAAAGTGA GTTTGGTTAT CCGGGAGAAG AAGATGATCC AGAAAACGGG 
GCTCCAGCTG CAGTGCCAGT TCCATTACCC GATATGGTTT TGCCACCTTC ATTTGATAGT 
GATAACTCAG CTTACCGATA CCGATATCTG GAACCCACTT CACAACTCCT AACCAGGCCA 
GTGTTGGATA CCCATGGTTG GGACCATGAC TGTGGATATG ACGGCGTCAA TGCAGAACAC 
AGTCTTGCTC TAGCTAGCCG GTTCCCTGCC ACAGCTACTG TCCAAGTCAC CAAGGACAAG 
AAAGAGTTCA ACATTCATCT GGACTCCTCT GTGTCTGCTA AGCACGGGGA GAATGGATCC 
ACCATGGCAG GGTTCGATAT TCAGAATGTA GGCAAGCAGC TGGCATATGT GGTCAGAGGA 
GAAACCAAAT TCAAGAATTT GAGGAAGAAC AAGACAACTG TTGGAGGGTC AGTGACATTC 
TTGGGAGAGA ACATCGCCAC TGGGGTCAAA CTCGAGGACC AAATAGCACT GGGGAAAAGG 
TTGGTGCTTG TGGGCAGCAC TGGGACAATG CGATCACAGG GAGATTCGGC CTATGGTGCG 
AACCTCGAGG TCAGGCTTAG GGAAGCTGAT TTCCCAATTG GACAGGACCA ATCTTCTTTT 
GGGCTGTCTC TGGTAAAGTG GAGAGGCGAT TTAGCCCTTG GAGCCAATCT CCAATCTCAA 
GTCTCTGTTG GAAGGAACTC AAAGATTGCG CTTCGTGCAG GACTTAACAA CAAGATGAGC 
GGACAGATCA CAGTCAGAAC CAGCAGCTCG GATCAGTTGC AAATCGCTCT CACAGCCATT 
CTTCCAATTG CCATGTCCAT CTACAAGAGC ATTCGACCCG AAGCGACGAA CGACAAGTAC 
AGCATGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOC159 ( NM_116485.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_567242.2 Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 159 (TOC159), mRNA.

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CloneID	OAb15601
Clone ID Related Accession (Same CDS sequence)	NM_001340383.1 , NM_116485.4 , NM_001340385.1 , NM_001340384.1
Accession Version	NM_116485.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	translocon at the outer envelope membrane of chloroplasts 159
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_116485.3.

ATGGACTCAA AGTCGGTTAC TCCAGAACCA ACCAACCCCT TCTACGCTTC TTCGGGGCAA 
TCAGGAAAAA CCTATGCTTC TGTTGTCGCC GCCGCTGCTG CTGCAGCCGC CGATAAGGAG 
GATGGTGGTG CTGTGAGTAG TGCCAAGGAG TTGGATTCCT CATCGGAGGC TGTGTCTGGT 
AATTCGGATA AGGTTGGAGC TGATGATTTA TCTGACTCCG AGAAGGAGAA GCCGAATTTG 
GTGGGTGATG GGAAGGTTTC CGACGAGGTG GATGGTTCTT TAAAGGAGGA TTCTACTACT 
CCTGAGGCTA CTCCGAAGCC TGAGGTGGTT TCTGGTGAGA CAATTGGTGT AGATGATGTT 
TCATCGTTAT CTCCGAAGCC GGAGGCTGTT TCTGATGGTG TAGGGGTTGT GGAGGAGAAT 
AAGAAGGTTA AGGAGGACGT GGAGGATATT AAAGACGATG GTGAGAGTAA GATTGAAAAT 
GGGAGTGTTG ATGTTGATGT GAAACAGGCT TCCACAGATG GGGAGAGTGA GAGTAAAGTG 
AAGGATGTGG AAGAAGAAGA TGTTGGAACA AAAAAAGATG ATGAGGGGGA GAGTGAGTTG 
GGTGGAAAAG TTGATGTTGA CGATAAAAGT GATAATGTTA TTGAAGAAGA GGGTGTGGAG 
TTAACTGATA AAGGAGACGT CATTGTGAAC TCGTCCCCTG TAGAATCAGT GCATGTTGAT 
GTGGCAAAAC CAGGGGTGGT TGTTGTAGGA GACGCAGAGG GAAGTGAGGA GTTGAAGATT 
AATGCTGATG CTGAAACTCT TGAAGTGGCT AATAAGTTTG ATCAAATTGG GGATGATGAT 
AGTGGTGAAT TTGAACCTGT GTCTGACAAG GCCATTGAGG AGGTAGAAGA AAAATTTACT 
AGCGAATCAG ACTCTATTGC AGACTCCTCT AAGTTAGAAT CTGTGGATAC CAGTGCTGTA 
GAGCCAGAGG TTGTAGCTGC CGAGAGTGGG AGTGAACCAA AGGATGTGGA AAAAGCCAAC 
GGATTGGAGA AGGGTATGAC TTATGCTGAA GTAATCAAGG CAGCTAGTGC AGTAGCTGAC 
AATGGAACCA AAGAGGAGGA GAGTGTGCTC GGTGGCATAG TCGATGATGC AGAAGAAGGT 
GTTAAGTTAA ACAACAAAGG AGACTTTGTG GTGGACTCAT CTGCTATTGA AGCTGTGAAT 
GTAGATGTGG CAAAGCCAGG GGTTGTTGTT GTTGGAGACG TAGAGGTGAG TGAGGTGCTG 
GAAACTGATG GCAACATCCC TGATGTGCAT AATAAGTTTG ATCCAATTGG CCAAGGTGAA 
GGTGGTGAAG TTGAACTTGA ATCCGATAAA GCCACAGAGG AGGGAGGAGG AAAGTTGGTT 
AGTGAAGGAG ACTCTATGGT TGACTCCTCT GTGGTGGACT CTGTTGATGC TGATATCAAT 
GTTGCAGAGC CAGGGGTTGT GGTTGTCGGG GCGGCTAAGG AGGCTGTAAT TAAGGAAGAT 
GATAAAGATG ATGAGGTTGA CAAGACCATC TCAAATATTG AGGAACCTGA TGACCTGACT 
GCTGCATATG ATGGAAATTT TGAATTGGCT GTTAAGGAAA TATCAGAGGC TGCCAAAGTA 
GAGCCAGACG AACCAAAAGT AGGTGTGGAA GTAGAAGAAT TGCCTGTTTC TGAAAGTTTA 
AAAGTGGGTT CAGTTGATGC AGAAGAGGAT TCAATTCCTG CAGCTGAGTC ACAGTTTGAG 
GTTCGGAAAG TTGTCGAAGG GGACAGTGCT GAAGAGGATG AAAACAAGCT CCCAGTCGAA 
GACATTGTTT CTTCTCGTGA GTTTAGCTTT GGAGGCAAGG AAGTGGACCA GGAACCTTCT 
GGCGAAGGTG TTACCAGGGT TGATGGGTCT GAAAGTGAAG AAGAAACTGA AGAGATGATA 
TTTGGGAGTT CTGAAGCTGC AAAACAGTTC TTAGCAGAGT TGGAAAAGGC TTCTTCTGGT 
ATAGAGGCAC ATTCTGATGA AGCAAACATT TCTAACAATA TGTCAGATAG GATTGATGGC 
CAGATTGTCA CCGATTCTGA CGAGGATGTA GACACAGAGG ATGAAGGTGA GGAGAAAATG 
TTTGATACTG CAGCTTTGGC TGCGCTTTTG AAGGCAGCCA CTGGTGGTGG AAGTTCAGAA 
GGTGGCAATT TTACCATAAC ATCTCAGGAT GGCACGAAGC TTTTCTCTAT GGATCGACCT 
GCTGGTTTGA GTTCATCGTT AAGGCCCTTG AAGCCTGCAG CAGCTCCACG TGCAAACCGT 
TCCAACATCT TTTCCAATTC TAATGTCACA ATGGCAGATG AGACTGAGAT TAACTTGAGC 
GAGGAAGAGA AACAGAAATT AGAAAAGTTG CAAAGCCTCC GTGTGAAGTT TTTGCGGCTT 
TTGCAAAGGT TGGGTCATTC GGCAGAAGAT TCAATTGCAG CGCAGGTTCT TTACCGTCTT 
GCACTACTTG CTGGGAGACA AGCAGGGCAG TTATTCAGTC TTGATGCTGC AAAGAAGAAG 
GCTGTGGAGT CTGAGGCTGA GGGCAACGAA GAATTGATCT TCTCCCTAAA CATACTGGTC 
CTTGGAAAAG CCGGGGTGGG AAAAAGTGCT ACTATAAATT CCATTTTGGG AAACCAGATT 
GCGTCCATTG ATGCTTTTGG GCTCTCAACC ACTTCGGTTA GAGAAATTTC TGGAACGGTG 
AATGGTGTCA AGATTACCTT TATTGATACT CCGGGTTTGA AGTCTGCTGC GATGGATCAA 
AGTACAAATG CAAAAATGTT GTCCTCTGTA AAGAAGGTAA TGAAGAAGTG TCCCCCTGAT 
ATTGTACTAT ATGTAGATCG TCTTGACACC CAGACCAGGG ACTTGAACAA TTTGCCCTTG 
CTAAGGACGA TTACTGCTTC TCTTGGTACA TCCATATGGA AAAACGCTAT AGTGACATTG 
ACCCATGCGG CTTCTGCCCC TCCTGATGGC CCATCTGGCA CCCCATTGAG CTATGATGTG 
TTTGTAGCGC AGTGCTCACA TATTGTGCAA CAGTCTATAG GACAGGCTGT TGGAGATCTA 
CGCTTGATGA ATCCAAGTCT GATGAATCCA GTTTCACTTG TTGAGAATCA CCCCTTGTGT 
AGGAAGAACC GGGAGGGAGT GAAGGTTCTC CCGAATGGCC AAACTTGGAG ATCTCAGCTG 
TTGCTATTGT GTTACTCCCT GAAGGTTTTG TCAGAAACAA ATTCTCTATT GAGGCCTCAA 
GAACCATTGG ACCATCGTAA AGTATTTGGT TTCCGAGTTA GATCCCCGCC TCTCCCTTAC 
TTGTTGTCTT GGCTGTTGCA GTCCCGTGCA CATCCTAAGC TCCCTGGAGA CCAAGGTGGT 
GATAGTGTTG ACTCTGATAT CGAAATTGAT GATGTGTCTG ATTCTGAGCA GGAAGATGGG 
GAAGATGATG AGTATGACCA GCTGCCACCA TTTAAGCCTC TTCGAAAAAC CCAACTTGCA 
AAGCTCTCAA ATGAACAGAG AAAGGCATAT TTCGAGGAGT ATGATTACCG TGTAAAGCTC 
CTGCAGAAGA AGCAATGGAG AGAGGAATTA AAGAGGATGA AAGAAATGAA GAAAAACGGG 
AAGAAGCTGG GTGAAAGTGA GTTTGGTTAT CCGGGAGAAG AAGATGATCC AGAAAACGGG 
GCTCCAGCTG CAGTGCCAGT TCCATTACCC GATATGGTTT TGCCACCTTC ATTTGATAGT 
GATAACTCAG CTTACCGATA CCGATATCTG GAACCCACTT CACAACTCCT AACCAGGCCA 
GTGTTGGATA CCCATGGTTG GGACCATGAC TGTGGATATG ACGGCGTCAA TGCAGAACAC 
AGTCTTGCTC TAGCTAGCCG GTTCCCTGCC ACAGCTACTG TCCAAGTCAC CAAGGACAAG 
AAAGAGTTCA ACATTCATCT GGACTCCTCT GTGTCTGCTA AGCACGGGGA GAATGGATCC 
ACCATGGCAG GGTTCGATAT TCAGAATGTA GGCAAGCAGC TGGCATATGT GGTCAGAGGA 
GAAACCAAAT TCAAGAATTT GAGGAAGAAC AAGACAACTG TTGGAGGGTC AGTGACATTC 
TTGGGAGAGA ACATCGCCAC TGGGGTCAAA CTCGAGGACC AAATAGCACT GGGGAAAAGG 
TTGGTGCTTG TGGGCAGCAC TGGGACAATG CGATCACAGG GAGATTCGGC CTATGGTGCG 
AACCTCGAGG TCAGGCTTAG GGAAGCTGAT TTCCCAATTG GACAGGACCA ATCTTCTTTT 
GGGCTGTCTC TGGTAAAGTG GAGAGGCGAT TTAGCCCTTG GAGCCAATCT CCAATCTCAA 
GTCTCTGTTG GAAGGAACTC AAAGATTGCG CTTCGTGCAG GACTTAACAA CAAGATGAGC 
GGACAGATCA CAGTCAGAAC CAGCAGCTCG GATCAGTTGC AAATCGCTCT CACAGCCATT 
CTTCCAATTG CCATGTCCAT CTACAAGAGC ATTCGACCCG AAGCGACGAA CGACAAGTAC 
AGCATGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_567242.2
CDS	317..4828
Translation	MDSKSVTPEPTNPFYASSGQSGKTYASVVAAAAAAAADKEDGGAVSSAKELDSSSEAVSGNSDKVGADDLSDSEKEKPNLVGDGKVSDEVDGSLKEDSTTPEATPKPEVVSGETIGVDDVSSLSPKPEAVSDGVGVVEENKKVKEDVEDIKDDGESKIENGSVDVDVKQASTDGESESKVKDVEEEDVGTKKDDEGESELGGKVDVDDKSDNVIEEEGVELTDKGDVIVNSSPVESVHVDVAKPGVVVVGDAEGSEELKINADAETLEVANKFDQIGDDDSGEFEPVSDKAIEEVEEKFTSESDSIADSSKLESVDTSAVEPEVVAAESGSEPKDVEKANGLEKGMTYAEVIKAASAVADNGTKEEESVLGGIVDDAEEGVKLNNKGDFVVDSSAIEAVNVDVAKPGVVVVGDVEVSEVLETDGNIPDVHNKFDPIGQGEGGEVELESDKATEEGGGKLVSEGDSMVDSSVVDSVDADINVAEPGVVVVGAAKEAVIKEDDKDDEVDKTISNIEEPDDLTAAYDGNFELAVKEISEAAKVEPDEPKVGVEVEELPVSESLKVGSVDAEEDSIPAAESQFEVRKVVEGDSAEEDENKLPVEDIVSSREFSFGGKEVDQEPSGEGVTRVDGSESEEETEEMIFGSSEAAKQFLAELEKASSGIEAHSDEANISNNMSDRIDGQIVTDSDEDVDTEDEGEEKMFDTAALAALLKAATGGGSSEGGNFTITSQDGTKLFSMDRPAGLSSSLRPLKPAAAPRANRSNIFSNSNVTMADETEINLSEEEKQKLEKLQSLRVKFLRLLQRLGHSAEDSIAAQVLYRLALLAGRQAGQLFSLDAAKKKAVESEAEGNEELIFSLNILVLGKAGVGKSATINSILGNQIASIDAFGLSTTSVREISGTVNGVKITFIDTPGLKSAAMDQSTNAKMLSSVKKVMKKCPPDIVLYVDRLDTQTRDLNNLPLLRTITASLGTSIWKNAIVTLTHAASAPPDGPSGTPLSYDVFVAQCSHIVQQSIGQAVGDLRLMNPSLMNPVSLVENHPLCRKNREGVKVLPNGQTWRSQLLLLCYSLKVLSETNSLLRPQEPLDHRKVFGFRVRSPPLPYLLSWLLQSRAHPKLPGDQGGDSVDSDIEIDDVSDSEQEDGEDDEYDQLPPFKPLRKTQLAKLSNEQRKAYFEEYDYRVKLLQKKQWREELKRMKEMKKNGKKLGESEFGYPGEEDDPENGAPAAVPVPLPDMVLPPSFDSDNSAYRYRYLEPTSQLLTRPVLDTHGWDHDCGYDGVNAEHSLALASRFPATATVQVTKDKKEFNIHLDSSVSAKHGENGSTMAGFDIQNVGKQLAYVVRGETKFKNLRKNKTTVGGSVTFLGENIATGVKLEDQIALGKRLVLVGSTGTMRSQGDSAYGANLEVRLREADFPIGQDQSSFGLSLVKWRGDLALGANLQSQVSVGRNSKIALRAGLNNKMSGQITVRTSSSDQLQIALTAILPIAMSIYKSIRPEATNDKYSMY

Target ORF information:

RefSeq Version	NM_116485.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 159 (TOC159), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_116485.4

ORF Insert Sequence:

ATGGACTCAA AGTCGGTTAC TCCAGAACCA ACCAACCCCT TCTACGCTTC TTCGGGGCAA 
TCAGGAAAAA CCTATGCTTC TGTTGTCGCC GCCGCTGCTG CTGCAGCCGC CGATAAGGAG 
GATGGTGGTG CTGTGAGTAG TGCCAAGGAG TTGGATTCCT CATCGGAGGC TGTGTCTGGT 
AATTCGGATA AGGTTGGAGC TGATGATTTA TCTGACTCCG AGAAGGAGAA GCCGAATTTG 
GTGGGTGATG GGAAGGTTTC CGACGAGGTG GATGGTTCTT TAAAGGAGGA TTCTACTACT 
CCTGAGGCTA CTCCGAAGCC TGAGGTGGTT TCTGGTGAGA CAATTGGTGT AGATGATGTT 
TCATCGTTAT CTCCGAAGCC GGAGGCTGTT TCTGATGGTG TAGGGGTTGT GGAGGAGAAT 
AAGAAGGTTA AGGAGGACGT GGAGGATATT AAAGACGATG GTGAGAGTAA GATTGAAAAT 
GGGAGTGTTG ATGTTGATGT GAAACAGGCT TCCACAGATG GGGAGAGTGA GAGTAAAGTG 
AAGGATGTGG AAGAAGAAGA TGTTGGAACA AAAAAAGATG ATGAGGGGGA GAGTGAGTTG 
GGTGGAAAAG TTGATGTTGA CGATAAAAGT GATAATGTTA TTGAAGAAGA GGGTGTGGAG 
TTAACTGATA AAGGAGACGT CATTGTGAAC TCGTCCCCTG TAGAATCAGT GCATGTTGAT 
GTGGCAAAAC CAGGGGTGGT TGTTGTAGGA GACGCAGAGG GAAGTGAGGA GTTGAAGATT 
AATGCTGATG CTGAAACTCT TGAAGTGGCT AATAAGTTTG ATCAAATTGG GGATGATGAT 
AGTGGTGAAT TTGAACCTGT GTCTGACAAG GCCATTGAGG AGGTAGAAGA AAAATTTACT 
AGCGAATCAG ACTCTATTGC AGACTCCTCT AAGTTAGAAT CTGTGGATAC CAGTGCTGTA 
GAGCCAGAGG TTGTAGCTGC CGAGAGTGGG AGTGAACCAA AGGATGTGGA AAAAGCCAAC 
GGATTGGAGA AGGGTATGAC TTATGCTGAA GTAATCAAGG CAGCTAGTGC AGTAGCTGAC 
AATGGAACCA AAGAGGAGGA GAGTGTGCTC GGTGGCATAG TCGATGATGC AGAAGAAGGT 
GTTAAGTTAA ACAACAAAGG AGACTTTGTG GTGGACTCAT CTGCTATTGA AGCTGTGAAT 
GTAGATGTGG CAAAGCCAGG GGTTGTTGTT GTTGGAGACG TAGAGGTGAG TGAGGTGCTG 
GAAACTGATG GCAACATCCC TGATGTGCAT AATAAGTTTG ATCCAATTGG CCAAGGTGAA 
GGTGGTGAAG TTGAACTTGA ATCCGATAAA GCCACAGAGG AGGGAGGAGG AAAGTTGGTT 
AGTGAAGGAG ACTCTATGGT TGACTCCTCT GTGGTGGACT CTGTTGATGC TGATATCAAT 
GTTGCAGAGC CAGGGGTTGT GGTTGTCGGG GCGGCTAAGG AGGCTGTAAT TAAGGAAGAT 
GATAAAGATG ATGAGGTTGA CAAGACCATC TCAAATATTG AGGAACCTGA TGACCTGACT 
GCTGCATATG ATGGAAATTT TGAATTGGCT GTTAAGGAAA TATCAGAGGC TGCCAAAGTA 
GAGCCAGACG AACCAAAAGT AGGTGTGGAA GTAGAAGAAT TGCCTGTTTC TGAAAGTTTA 
AAAGTGGGTT CAGTTGATGC AGAAGAGGAT TCAATTCCTG CAGCTGAGTC ACAGTTTGAG 
GTTCGGAAAG TTGTCGAAGG GGACAGTGCT GAAGAGGATG AAAACAAGCT CCCAGTCGAA 
GACATTGTTT CTTCTCGTGA GTTTAGCTTT GGAGGCAAGG AAGTGGACCA GGAACCTTCT 
GGCGAAGGTG TTACCAGGGT TGATGGGTCT GAAAGTGAAG AAGAAACTGA AGAGATGATA 
TTTGGGAGTT CTGAAGCTGC AAAACAGTTC TTAGCAGAGT TGGAAAAGGC TTCTTCTGGT 
ATAGAGGCAC ATTCTGATGA AGCAAACATT TCTAACAATA TGTCAGATAG GATTGATGGC 
CAGATTGTCA CCGATTCTGA CGAGGATGTA GACACAGAGG ATGAAGGTGA GGAGAAAATG 
TTTGATACTG CAGCTTTGGC TGCGCTTTTG AAGGCAGCCA CTGGTGGTGG AAGTTCAGAA 
GGTGGCAATT TTACCATAAC ATCTCAGGAT GGCACGAAGC TTTTCTCTAT GGATCGACCT 
GCTGGTTTGA GTTCATCGTT AAGGCCCTTG AAGCCTGCAG CAGCTCCACG TGCAAACCGT 
TCCAACATCT TTTCCAATTC TAATGTCACA ATGGCAGATG AGACTGAGAT TAACTTGAGC 
GAGGAAGAGA AACAGAAATT AGAAAAGTTG CAAAGCCTCC GTGTGAAGTT TTTGCGGCTT 
TTGCAAAGGT TGGGTCATTC GGCAGAAGAT TCAATTGCAG CGCAGGTTCT TTACCGTCTT 
GCACTACTTG CTGGGAGACA AGCAGGGCAG TTATTCAGTC TTGATGCTGC AAAGAAGAAG 
GCTGTGGAGT CTGAGGCTGA GGGCAACGAA GAATTGATCT TCTCCCTAAA CATACTGGTC 
CTTGGAAAAG CCGGGGTGGG AAAAAGTGCT ACTATAAATT CCATTTTGGG AAACCAGATT 
GCGTCCATTG ATGCTTTTGG GCTCTCAACC ACTTCGGTTA GAGAAATTTC TGGAACGGTG 
AATGGTGTCA AGATTACCTT TATTGATACT CCGGGTTTGA AGTCTGCTGC GATGGATCAA 
AGTACAAATG CAAAAATGTT GTCCTCTGTA AAGAAGGTAA TGAAGAAGTG TCCCCCTGAT 
ATTGTACTAT ATGTAGATCG TCTTGACACC CAGACCAGGG ACTTGAACAA TTTGCCCTTG 
CTAAGGACGA TTACTGCTTC TCTTGGTACA TCCATATGGA AAAACGCTAT AGTGACATTG 
ACCCATGCGG CTTCTGCCCC TCCTGATGGC CCATCTGGCA CCCCATTGAG CTATGATGTG 
TTTGTAGCGC AGTGCTCACA TATTGTGCAA CAGTCTATAG GACAGGCTGT TGGAGATCTA 
CGCTTGATGA ATCCAAGTCT GATGAATCCA GTTTCACTTG TTGAGAATCA CCCCTTGTGT 
AGGAAGAACC GGGAGGGAGT GAAGGTTCTC CCGAATGGCC AAACTTGGAG ATCTCAGCTG 
TTGCTATTGT GTTACTCCCT GAAGGTTTTG TCAGAAACAA ATTCTCTATT GAGGCCTCAA 
GAACCATTGG ACCATCGTAA AGTATTTGGT TTCCGAGTTA GATCCCCGCC TCTCCCTTAC 
TTGTTGTCTT GGCTGTTGCA GTCCCGTGCA CATCCTAAGC TCCCTGGAGA CCAAGGTGGT 
GATAGTGTTG ACTCTGATAT CGAAATTGAT GATGTGTCTG ATTCTGAGCA GGAAGATGGG 
GAAGATGATG AGTATGACCA GCTGCCACCA TTTAAGCCTC TTCGAAAAAC CCAACTTGCA 
AAGCTCTCAA ATGAACAGAG AAAGGCATAT TTCGAGGAGT ATGATTACCG TGTAAAGCTC 
CTGCAGAAGA AGCAATGGAG AGAGGAATTA AAGAGGATGA AAGAAATGAA GAAAAACGGG 
AAGAAGCTGG GTGAAAGTGA GTTTGGTTAT CCGGGAGAAG AAGATGATCC AGAAAACGGG 
GCTCCAGCTG CAGTGCCAGT TCCATTACCC GATATGGTTT TGCCACCTTC ATTTGATAGT 
GATAACTCAG CTTACCGATA CCGATATCTG GAACCCACTT CACAACTCCT AACCAGGCCA 
GTGTTGGATA CCCATGGTTG GGACCATGAC TGTGGATATG ACGGCGTCAA TGCAGAACAC 
AGTCTTGCTC TAGCTAGCCG GTTCCCTGCC ACAGCTACTG TCCAAGTCAC CAAGGACAAG 
AAAGAGTTCA ACATTCATCT GGACTCCTCT GTGTCTGCTA AGCACGGGGA GAATGGATCC 
ACCATGGCAG GGTTCGATAT TCAGAATGTA GGCAAGCAGC TGGCATATGT GGTCAGAGGA 
GAAACCAAAT TCAAGAATTT GAGGAAGAAC AAGACAACTG TTGGAGGGTC AGTGACATTC 
TTGGGAGAGA ACATCGCCAC TGGGGTCAAA CTCGAGGACC AAATAGCACT GGGGAAAAGG 
TTGGTGCTTG TGGGCAGCAC TGGGACAATG CGATCACAGG GAGATTCGGC CTATGGTGCG 
AACCTCGAGG TCAGGCTTAG GGAAGCTGAT TTCCCAATTG GACAGGACCA ATCTTCTTTT 
GGGCTGTCTC TGGTAAAGTG GAGAGGCGAT TTAGCCCTTG GAGCCAATCT CCAATCTCAA 
GTCTCTGTTG GAAGGAACTC AAAGATTGCG CTTCGTGCAG GACTTAACAA CAAGATGAGC 
GGACAGATCA CAGTCAGAAC CAGCAGCTCG GATCAGTTGC AAATCGCTCT CACAGCCATT 
CTTCCAATTG CCATGTCCAT CTACAAGAGC ATTCGACCCG AAGCGACGAA CGACAAGTAC 
AGCATGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOC159 ( NM_001340385.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001328635.1 Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 159 (TOC159), mRNA.

Price & Availability↑

CloneID	OAb15601
Clone ID Related Accession (Same CDS sequence)	NM_001340383.1 , NM_116485.4 , NM_001340385.1 , NM_001340384.1
Accession Version	NM_001340385.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	translocon at the outer envelope membrane of chloroplasts 159
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGACTCAA AGTCGGTTAC TCCAGAACCA ACCAACCCCT TCTACGCTTC TTCGGGGCAA 
TCAGGAAAAA CCTATGCTTC TGTTGTCGCC GCCGCTGCTG CTGCAGCCGC CGATAAGGAG 
GATGGTGGTG CTGTGAGTAG TGCCAAGGAG TTGGATTCCT CATCGGAGGC TGTGTCTGGT 
AATTCGGATA AGGTTGGAGC TGATGATTTA TCTGACTCCG AGAAGGAGAA GCCGAATTTG 
GTGGGTGATG GGAAGGTTTC CGACGAGGTG GATGGTTCTT TAAAGGAGGA TTCTACTACT 
CCTGAGGCTA CTCCGAAGCC TGAGGTGGTT TCTGGTGAGA CAATTGGTGT AGATGATGTT 
TCATCGTTAT CTCCGAAGCC GGAGGCTGTT TCTGATGGTG TAGGGGTTGT GGAGGAGAAT 
AAGAAGGTTA AGGAGGACGT GGAGGATATT AAAGACGATG GTGAGAGTAA GATTGAAAAT 
GGGAGTGTTG ATGTTGATGT GAAACAGGCT TCCACAGATG GGGAGAGTGA GAGTAAAGTG 
AAGGATGTGG AAGAAGAAGA TGTTGGAACA AAAAAAGATG ATGAGGGGGA GAGTGAGTTG 
GGTGGAAAAG TTGATGTTGA CGATAAAAGT GATAATGTTA TTGAAGAAGA GGGTGTGGAG 
TTAACTGATA AAGGAGACGT CATTGTGAAC TCGTCCCCTG TAGAATCAGT GCATGTTGAT 
GTGGCAAAAC CAGGGGTGGT TGTTGTAGGA GACGCAGAGG GAAGTGAGGA GTTGAAGATT 
AATGCTGATG CTGAAACTCT TGAAGTGGCT AATAAGTTTG ATCAAATTGG GGATGATGAT 
AGTGGTGAAT TTGAACCTGT GTCTGACAAG GCCATTGAGG AGGTAGAAGA AAAATTTACT 
AGCGAATCAG ACTCTATTGC AGACTCCTCT AAGTTAGAAT CTGTGGATAC CAGTGCTGTA 
GAGCCAGAGG TTGTAGCTGC CGAGAGTGGG AGTGAACCAA AGGATGTGGA AAAAGCCAAC 
GGATTGGAGA AGGGTATGAC TTATGCTGAA GTAATCAAGG CAGCTAGTGC AGTAGCTGAC 
AATGGAACCA AAGAGGAGGA GAGTGTGCTC GGTGGCATAG TCGATGATGC AGAAGAAGGT 
GTTAAGTTAA ACAACAAAGG AGACTTTGTG GTGGACTCAT CTGCTATTGA AGCTGTGAAT 
GTAGATGTGG CAAAGCCAGG GGTTGTTGTT GTTGGAGACG TAGAGGTGAG TGAGGTGCTG 
GAAACTGATG GCAACATCCC TGATGTGCAT AATAAGTTTG ATCCAATTGG CCAAGGTGAA 
GGTGGTGAAG TTGAACTTGA ATCCGATAAA GCCACAGAGG AGGGAGGAGG AAAGTTGGTT 
AGTGAAGGAG ACTCTATGGT TGACTCCTCT GTGGTGGACT CTGTTGATGC TGATATCAAT 
GTTGCAGAGC CAGGGGTTGT GGTTGTCGGG GCGGCTAAGG AGGCTGTAAT TAAGGAAGAT 
GATAAAGATG ATGAGGTTGA CAAGACCATC TCAAATATTG AGGAACCTGA TGACCTGACT 
GCTGCATATG ATGGAAATTT TGAATTGGCT GTTAAGGAAA TATCAGAGGC TGCCAAAGTA 
GAGCCAGACG AACCAAAAGT AGGTGTGGAA GTAGAAGAAT TGCCTGTTTC TGAAAGTTTA 
AAAGTGGGTT CAGTTGATGC AGAAGAGGAT TCAATTCCTG CAGCTGAGTC ACAGTTTGAG 
GTTCGGAAAG TTGTCGAAGG GGACAGTGCT GAAGAGGATG AAAACAAGCT CCCAGTCGAA 
GACATTGTTT CTTCTCGTGA GTTTAGCTTT GGAGGCAAGG AAGTGGACCA GGAACCTTCT 
GGCGAAGGTG TTACCAGGGT TGATGGGTCT GAAAGTGAAG AAGAAACTGA AGAGATGATA 
TTTGGGAGTT CTGAAGCTGC AAAACAGTTC TTAGCAGAGT TGGAAAAGGC TTCTTCTGGT 
ATAGAGGCAC ATTCTGATGA AGCAAACATT TCTAACAATA TGTCAGATAG GATTGATGGC 
CAGATTGTCA CCGATTCTGA CGAGGATGTA GACACAGAGG ATGAAGGTGA GGAGAAAATG 
TTTGATACTG CAGCTTTGGC TGCGCTTTTG AAGGCAGCCA CTGGTGGTGG AAGTTCAGAA 
GGTGGCAATT TTACCATAAC ATCTCAGGAT GGCACGAAGC TTTTCTCTAT GGATCGACCT 
GCTGGTTTGA GTTCATCGTT AAGGCCCTTG AAGCCTGCAG CAGCTCCACG TGCAAACCGT 
TCCAACATCT TTTCCAATTC TAATGTCACA ATGGCAGATG AGACTGAGAT TAACTTGAGC 
GAGGAAGAGA AACAGAAATT AGAAAAGTTG CAAAGCCTCC GTGTGAAGTT TTTGCGGCTT 
TTGCAAAGGT TGGGTCATTC GGCAGAAGAT TCAATTGCAG CGCAGGTTCT TTACCGTCTT 
GCACTACTTG CTGGGAGACA AGCAGGGCAG TTATTCAGTC TTGATGCTGC AAAGAAGAAG 
GCTGTGGAGT CTGAGGCTGA GGGCAACGAA GAATTGATCT TCTCCCTAAA CATACTGGTC 
CTTGGAAAAG CCGGGGTGGG AAAAAGTGCT ACTATAAATT CCATTTTGGG AAACCAGATT 
GCGTCCATTG ATGCTTTTGG GCTCTCAACC ACTTCGGTTA GAGAAATTTC TGGAACGGTG 
AATGGTGTCA AGATTACCTT TATTGATACT CCGGGTTTGA AGTCTGCTGC GATGGATCAA 
AGTACAAATG CAAAAATGTT GTCCTCTGTA AAGAAGGTAA TGAAGAAGTG TCCCCCTGAT 
ATTGTACTAT ATGTAGATCG TCTTGACACC CAGACCAGGG ACTTGAACAA TTTGCCCTTG 
CTAAGGACGA TTACTGCTTC TCTTGGTACA TCCATATGGA AAAACGCTAT AGTGACATTG 
ACCCATGCGG CTTCTGCCCC TCCTGATGGC CCATCTGGCA CCCCATTGAG CTATGATGTG 
TTTGTAGCGC AGTGCTCACA TATTGTGCAA CAGTCTATAG GACAGGCTGT TGGAGATCTA 
CGCTTGATGA ATCCAAGTCT GATGAATCCA GTTTCACTTG TTGAGAATCA CCCCTTGTGT 
AGGAAGAACC GGGAGGGAGT GAAGGTTCTC CCGAATGGCC AAACTTGGAG ATCTCAGCTG 
TTGCTATTGT GTTACTCCCT GAAGGTTTTG TCAGAAACAA ATTCTCTATT GAGGCCTCAA 
GAACCATTGG ACCATCGTAA AGTATTTGGT TTCCGAGTTA GATCCCCGCC TCTCCCTTAC 
TTGTTGTCTT GGCTGTTGCA GTCCCGTGCA CATCCTAAGC TCCCTGGAGA CCAAGGTGGT 
GATAGTGTTG ACTCTGATAT CGAAATTGAT GATGTGTCTG ATTCTGAGCA GGAAGATGGG 
GAAGATGATG AGTATGACCA GCTGCCACCA TTTAAGCCTC TTCGAAAAAC CCAACTTGCA 
AAGCTCTCAA ATGAACAGAG AAAGGCATAT TTCGAGGAGT ATGATTACCG TGTAAAGCTC 
CTGCAGAAGA AGCAATGGAG AGAGGAATTA AAGAGGATGA AAGAAATGAA GAAAAACGGG 
AAGAAGCTGG GTGAAAGTGA GTTTGGTTAT CCGGGAGAAG AAGATGATCC AGAAAACGGG 
GCTCCAGCTG CAGTGCCAGT TCCATTACCC GATATGGTTT TGCCACCTTC ATTTGATAGT 
GATAACTCAG CTTACCGATA CCGATATCTG GAACCCACTT CACAACTCCT AACCAGGCCA 
GTGTTGGATA CCCATGGTTG GGACCATGAC TGTGGATATG ACGGCGTCAA TGCAGAACAC 
AGTCTTGCTC TAGCTAGCCG GTTCCCTGCC ACAGCTACTG TCCAAGTCAC CAAGGACAAG 
AAAGAGTTCA ACATTCATCT GGACTCCTCT GTGTCTGCTA AGCACGGGGA GAATGGATCC 
ACCATGGCAG GGTTCGATAT TCAGAATGTA GGCAAGCAGC TGGCATATGT GGTCAGAGGA 
GAAACCAAAT TCAAGAATTT GAGGAAGAAC AAGACAACTG TTGGAGGGTC AGTGACATTC 
TTGGGAGAGA ACATCGCCAC TGGGGTCAAA CTCGAGGACC AAATAGCACT GGGGAAAAGG 
TTGGTGCTTG TGGGCAGCAC TGGGACAATG CGATCACAGG GAGATTCGGC CTATGGTGCG 
AACCTCGAGG TCAGGCTTAG GGAAGCTGAT TTCCCAATTG GACAGGACCA ATCTTCTTTT 
GGGCTGTCTC TGGTAAAGTG GAGAGGCGAT TTAGCCCTTG GAGCCAATCT CCAATCTCAA 
GTCTCTGTTG GAAGGAACTC AAAGATTGCG CTTCGTGCAG GACTTAACAA CAAGATGAGC 
GGACAGATCA CAGTCAGAAC CAGCAGCTCG GATCAGTTGC AAATCGCTCT CACAGCCATT 
CTTCCAATTG CCATGTCCAT CTACAAGAGC ATTCGACCCG AAGCGACGAA CGACAAGTAC 
AGCATGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001328635.1
CDS	201..4712
Translation	MDSKSVTPEPTNPFYASSGQSGKTYASVVAAAAAAAADKEDGGAVSSAKELDSSSEAVSGNSDKVGADDLSDSEKEKPNLVGDGKVSDEVDGSLKEDSTTPEATPKPEVVSGETIGVDDVSSLSPKPEAVSDGVGVVEENKKVKEDVEDIKDDGESKIENGSVDVDVKQASTDGESESKVKDVEEEDVGTKKDDEGESELGGKVDVDDKSDNVIEEEGVELTDKGDVIVNSSPVESVHVDVAKPGVVVVGDAEGSEELKINADAETLEVANKFDQIGDDDSGEFEPVSDKAIEEVEEKFTSESDSIADSSKLESVDTSAVEPEVVAAESGSEPKDVEKANGLEKGMTYAEVIKAASAVADNGTKEEESVLGGIVDDAEEGVKLNNKGDFVVDSSAIEAVNVDVAKPGVVVVGDVEVSEVLETDGNIPDVHNKFDPIGQGEGGEVELESDKATEEGGGKLVSEGDSMVDSSVVDSVDADINVAEPGVVVVGAAKEAVIKEDDKDDEVDKTISNIEEPDDLTAAYDGNFELAVKEISEAAKVEPDEPKVGVEVEELPVSESLKVGSVDAEEDSIPAAESQFEVRKVVEGDSAEEDENKLPVEDIVSSREFSFGGKEVDQEPSGEGVTRVDGSESEEETEEMIFGSSEAAKQFLAELEKASSGIEAHSDEANISNNMSDRIDGQIVTDSDEDVDTEDEGEEKMFDTAALAALLKAATGGGSSEGGNFTITSQDGTKLFSMDRPAGLSSSLRPLKPAAAPRANRSNIFSNSNVTMADETEINLSEEEKQKLEKLQSLRVKFLRLLQRLGHSAEDSIAAQVLYRLALLAGRQAGQLFSLDAAKKKAVESEAEGNEELIFSLNILVLGKAGVGKSATINSILGNQIASIDAFGLSTTSVREISGTVNGVKITFIDTPGLKSAAMDQSTNAKMLSSVKKVMKKCPPDIVLYVDRLDTQTRDLNNLPLLRTITASLGTSIWKNAIVTLTHAASAPPDGPSGTPLSYDVFVAQCSHIVQQSIGQAVGDLRLMNPSLMNPVSLVENHPLCRKNREGVKVLPNGQTWRSQLLLLCYSLKVLSETNSLLRPQEPLDHRKVFGFRVRSPPLPYLLSWLLQSRAHPKLPGDQGGDSVDSDIEIDDVSDSEQEDGEDDEYDQLPPFKPLRKTQLAKLSNEQRKAYFEEYDYRVKLLQKKQWREELKRMKEMKKNGKKLGESEFGYPGEEDDPENGAPAAVPVPLPDMVLPPSFDSDNSAYRYRYLEPTSQLLTRPVLDTHGWDHDCGYDGVNAEHSLALASRFPATATVQVTKDKKEFNIHLDSSVSAKHGENGSTMAGFDIQNVGKQLAYVVRGETKFKNLRKNKTTVGGSVTFLGENIATGVKLEDQIALGKRLVLVGSTGTMRSQGDSAYGANLEVRLREADFPIGQDQSSFGLSLVKWRGDLALGANLQSQVSVGRNSKIALRAGLNNKMSGQITVRTSSSDQLQIALTAILPIAMSIYKSIRPEATNDKYSMY

Target ORF information:

RefSeq Version	NM_001340385.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 159 (TOC159), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001340385.1

ORF Insert Sequence:

ATGGACTCAA AGTCGGTTAC TCCAGAACCA ACCAACCCCT TCTACGCTTC TTCGGGGCAA 
TCAGGAAAAA CCTATGCTTC TGTTGTCGCC GCCGCTGCTG CTGCAGCCGC CGATAAGGAG 
GATGGTGGTG CTGTGAGTAG TGCCAAGGAG TTGGATTCCT CATCGGAGGC TGTGTCTGGT 
AATTCGGATA AGGTTGGAGC TGATGATTTA TCTGACTCCG AGAAGGAGAA GCCGAATTTG 
GTGGGTGATG GGAAGGTTTC CGACGAGGTG GATGGTTCTT TAAAGGAGGA TTCTACTACT 
CCTGAGGCTA CTCCGAAGCC TGAGGTGGTT TCTGGTGAGA CAATTGGTGT AGATGATGTT 
TCATCGTTAT CTCCGAAGCC GGAGGCTGTT TCTGATGGTG TAGGGGTTGT GGAGGAGAAT 
AAGAAGGTTA AGGAGGACGT GGAGGATATT AAAGACGATG GTGAGAGTAA GATTGAAAAT 
GGGAGTGTTG ATGTTGATGT GAAACAGGCT TCCACAGATG GGGAGAGTGA GAGTAAAGTG 
AAGGATGTGG AAGAAGAAGA TGTTGGAACA AAAAAAGATG ATGAGGGGGA GAGTGAGTTG 
GGTGGAAAAG TTGATGTTGA CGATAAAAGT GATAATGTTA TTGAAGAAGA GGGTGTGGAG 
TTAACTGATA AAGGAGACGT CATTGTGAAC TCGTCCCCTG TAGAATCAGT GCATGTTGAT 
GTGGCAAAAC CAGGGGTGGT TGTTGTAGGA GACGCAGAGG GAAGTGAGGA GTTGAAGATT 
AATGCTGATG CTGAAACTCT TGAAGTGGCT AATAAGTTTG ATCAAATTGG GGATGATGAT 
AGTGGTGAAT TTGAACCTGT GTCTGACAAG GCCATTGAGG AGGTAGAAGA AAAATTTACT 
AGCGAATCAG ACTCTATTGC AGACTCCTCT AAGTTAGAAT CTGTGGATAC CAGTGCTGTA 
GAGCCAGAGG TTGTAGCTGC CGAGAGTGGG AGTGAACCAA AGGATGTGGA AAAAGCCAAC 
GGATTGGAGA AGGGTATGAC TTATGCTGAA GTAATCAAGG CAGCTAGTGC AGTAGCTGAC 
AATGGAACCA AAGAGGAGGA GAGTGTGCTC GGTGGCATAG TCGATGATGC AGAAGAAGGT 
GTTAAGTTAA ACAACAAAGG AGACTTTGTG GTGGACTCAT CTGCTATTGA AGCTGTGAAT 
GTAGATGTGG CAAAGCCAGG GGTTGTTGTT GTTGGAGACG TAGAGGTGAG TGAGGTGCTG 
GAAACTGATG GCAACATCCC TGATGTGCAT AATAAGTTTG ATCCAATTGG CCAAGGTGAA 
GGTGGTGAAG TTGAACTTGA ATCCGATAAA GCCACAGAGG AGGGAGGAGG AAAGTTGGTT 
AGTGAAGGAG ACTCTATGGT TGACTCCTCT GTGGTGGACT CTGTTGATGC TGATATCAAT 
GTTGCAGAGC CAGGGGTTGT GGTTGTCGGG GCGGCTAAGG AGGCTGTAAT TAAGGAAGAT 
GATAAAGATG ATGAGGTTGA CAAGACCATC TCAAATATTG AGGAACCTGA TGACCTGACT 
GCTGCATATG ATGGAAATTT TGAATTGGCT GTTAAGGAAA TATCAGAGGC TGCCAAAGTA 
GAGCCAGACG AACCAAAAGT AGGTGTGGAA GTAGAAGAAT TGCCTGTTTC TGAAAGTTTA 
AAAGTGGGTT CAGTTGATGC AGAAGAGGAT TCAATTCCTG CAGCTGAGTC ACAGTTTGAG 
GTTCGGAAAG TTGTCGAAGG GGACAGTGCT GAAGAGGATG AAAACAAGCT CCCAGTCGAA 
GACATTGTTT CTTCTCGTGA GTTTAGCTTT GGAGGCAAGG AAGTGGACCA GGAACCTTCT 
GGCGAAGGTG TTACCAGGGT TGATGGGTCT GAAAGTGAAG AAGAAACTGA AGAGATGATA 
TTTGGGAGTT CTGAAGCTGC AAAACAGTTC TTAGCAGAGT TGGAAAAGGC TTCTTCTGGT 
ATAGAGGCAC ATTCTGATGA AGCAAACATT TCTAACAATA TGTCAGATAG GATTGATGGC 
CAGATTGTCA CCGATTCTGA CGAGGATGTA GACACAGAGG ATGAAGGTGA GGAGAAAATG 
TTTGATACTG CAGCTTTGGC TGCGCTTTTG AAGGCAGCCA CTGGTGGTGG AAGTTCAGAA 
GGTGGCAATT TTACCATAAC ATCTCAGGAT GGCACGAAGC TTTTCTCTAT GGATCGACCT 
GCTGGTTTGA GTTCATCGTT AAGGCCCTTG AAGCCTGCAG CAGCTCCACG TGCAAACCGT 
TCCAACATCT TTTCCAATTC TAATGTCACA ATGGCAGATG AGACTGAGAT TAACTTGAGC 
GAGGAAGAGA AACAGAAATT AGAAAAGTTG CAAAGCCTCC GTGTGAAGTT TTTGCGGCTT 
TTGCAAAGGT TGGGTCATTC GGCAGAAGAT TCAATTGCAG CGCAGGTTCT TTACCGTCTT 
GCACTACTTG CTGGGAGACA AGCAGGGCAG TTATTCAGTC TTGATGCTGC AAAGAAGAAG 
GCTGTGGAGT CTGAGGCTGA GGGCAACGAA GAATTGATCT TCTCCCTAAA CATACTGGTC 
CTTGGAAAAG CCGGGGTGGG AAAAAGTGCT ACTATAAATT CCATTTTGGG AAACCAGATT 
GCGTCCATTG ATGCTTTTGG GCTCTCAACC ACTTCGGTTA GAGAAATTTC TGGAACGGTG 
AATGGTGTCA AGATTACCTT TATTGATACT CCGGGTTTGA AGTCTGCTGC GATGGATCAA 
AGTACAAATG CAAAAATGTT GTCCTCTGTA AAGAAGGTAA TGAAGAAGTG TCCCCCTGAT 
ATTGTACTAT ATGTAGATCG TCTTGACACC CAGACCAGGG ACTTGAACAA TTTGCCCTTG 
CTAAGGACGA TTACTGCTTC TCTTGGTACA TCCATATGGA AAAACGCTAT AGTGACATTG 
ACCCATGCGG CTTCTGCCCC TCCTGATGGC CCATCTGGCA CCCCATTGAG CTATGATGTG 
TTTGTAGCGC AGTGCTCACA TATTGTGCAA CAGTCTATAG GACAGGCTGT TGGAGATCTA 
CGCTTGATGA ATCCAAGTCT GATGAATCCA GTTTCACTTG TTGAGAATCA CCCCTTGTGT 
AGGAAGAACC GGGAGGGAGT GAAGGTTCTC CCGAATGGCC AAACTTGGAG ATCTCAGCTG 
TTGCTATTGT GTTACTCCCT GAAGGTTTTG TCAGAAACAA ATTCTCTATT GAGGCCTCAA 
GAACCATTGG ACCATCGTAA AGTATTTGGT TTCCGAGTTA GATCCCCGCC TCTCCCTTAC 
TTGTTGTCTT GGCTGTTGCA GTCCCGTGCA CATCCTAAGC TCCCTGGAGA CCAAGGTGGT 
GATAGTGTTG ACTCTGATAT CGAAATTGAT GATGTGTCTG ATTCTGAGCA GGAAGATGGG 
GAAGATGATG AGTATGACCA GCTGCCACCA TTTAAGCCTC TTCGAAAAAC CCAACTTGCA 
AAGCTCTCAA ATGAACAGAG AAAGGCATAT TTCGAGGAGT ATGATTACCG TGTAAAGCTC 
CTGCAGAAGA AGCAATGGAG AGAGGAATTA AAGAGGATGA AAGAAATGAA GAAAAACGGG 
AAGAAGCTGG GTGAAAGTGA GTTTGGTTAT CCGGGAGAAG AAGATGATCC AGAAAACGGG 
GCTCCAGCTG CAGTGCCAGT TCCATTACCC GATATGGTTT TGCCACCTTC ATTTGATAGT 
GATAACTCAG CTTACCGATA CCGATATCTG GAACCCACTT CACAACTCCT AACCAGGCCA 
GTGTTGGATA CCCATGGTTG GGACCATGAC TGTGGATATG ACGGCGTCAA TGCAGAACAC 
AGTCTTGCTC TAGCTAGCCG GTTCCCTGCC ACAGCTACTG TCCAAGTCAC CAAGGACAAG 
AAAGAGTTCA ACATTCATCT GGACTCCTCT GTGTCTGCTA AGCACGGGGA GAATGGATCC 
ACCATGGCAG GGTTCGATAT TCAGAATGTA GGCAAGCAGC TGGCATATGT GGTCAGAGGA 
GAAACCAAAT TCAAGAATTT GAGGAAGAAC AAGACAACTG TTGGAGGGTC AGTGACATTC 
TTGGGAGAGA ACATCGCCAC TGGGGTCAAA CTCGAGGACC AAATAGCACT GGGGAAAAGG 
TTGGTGCTTG TGGGCAGCAC TGGGACAATG CGATCACAGG GAGATTCGGC CTATGGTGCG 
AACCTCGAGG TCAGGCTTAG GGAAGCTGAT TTCCCAATTG GACAGGACCA ATCTTCTTTT 
GGGCTGTCTC TGGTAAAGTG GAGAGGCGAT TTAGCCCTTG GAGCCAATCT CCAATCTCAA 
GTCTCTGTTG GAAGGAACTC AAAGATTGCG CTTCGTGCAG GACTTAACAA CAAGATGAGC 
GGACAGATCA CAGTCAGAAC CAGCAGCTCG GATCAGTTGC AAATCGCTCT CACAGCCATT 
CTTCCAATTG CCATGTCCAT CTACAAGAGC ATTCGACCCG AAGCGACGAA CGACAAGTAC 
AGCATGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOC159 ( NM_001340384.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001328636.1 Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 159 (TOC159), mRNA.

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CloneID	OAb15601
Clone ID Related Accession (Same CDS sequence)	NM_001340383.1 , NM_116485.4 , NM_001340385.1 , NM_001340384.1
Accession Version	NM_001340384.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	translocon at the outer envelope membrane of chloroplasts 159
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGACTCAA AGTCGGTTAC TCCAGAACCA ACCAACCCCT TCTACGCTTC TTCGGGGCAA 
TCAGGAAAAA CCTATGCTTC TGTTGTCGCC GCCGCTGCTG CTGCAGCCGC CGATAAGGAG 
GATGGTGGTG CTGTGAGTAG TGCCAAGGAG TTGGATTCCT CATCGGAGGC TGTGTCTGGT 
AATTCGGATA AGGTTGGAGC TGATGATTTA TCTGACTCCG AGAAGGAGAA GCCGAATTTG 
GTGGGTGATG GGAAGGTTTC CGACGAGGTG GATGGTTCTT TAAAGGAGGA TTCTACTACT 
CCTGAGGCTA CTCCGAAGCC TGAGGTGGTT TCTGGTGAGA CAATTGGTGT AGATGATGTT 
TCATCGTTAT CTCCGAAGCC GGAGGCTGTT TCTGATGGTG TAGGGGTTGT GGAGGAGAAT 
AAGAAGGTTA AGGAGGACGT GGAGGATATT AAAGACGATG GTGAGAGTAA GATTGAAAAT 
GGGAGTGTTG ATGTTGATGT GAAACAGGCT TCCACAGATG GGGAGAGTGA GAGTAAAGTG 
AAGGATGTGG AAGAAGAAGA TGTTGGAACA AAAAAAGATG ATGAGGGGGA GAGTGAGTTG 
GGTGGAAAAG TTGATGTTGA CGATAAAAGT GATAATGTTA TTGAAGAAGA GGGTGTGGAG 
TTAACTGATA AAGGAGACGT CATTGTGAAC TCGTCCCCTG TAGAATCAGT GCATGTTGAT 
GTGGCAAAAC CAGGGGTGGT TGTTGTAGGA GACGCAGAGG GAAGTGAGGA GTTGAAGATT 
AATGCTGATG CTGAAACTCT TGAAGTGGCT AATAAGTTTG ATCAAATTGG GGATGATGAT 
AGTGGTGAAT TTGAACCTGT GTCTGACAAG GCCATTGAGG AGGTAGAAGA AAAATTTACT 
AGCGAATCAG ACTCTATTGC AGACTCCTCT AAGTTAGAAT CTGTGGATAC CAGTGCTGTA 
GAGCCAGAGG TTGTAGCTGC CGAGAGTGGG AGTGAACCAA AGGATGTGGA AAAAGCCAAC 
GGATTGGAGA AGGGTATGAC TTATGCTGAA GTAATCAAGG CAGCTAGTGC AGTAGCTGAC 
AATGGAACCA AAGAGGAGGA GAGTGTGCTC GGTGGCATAG TCGATGATGC AGAAGAAGGT 
GTTAAGTTAA ACAACAAAGG AGACTTTGTG GTGGACTCAT CTGCTATTGA AGCTGTGAAT 
GTAGATGTGG CAAAGCCAGG GGTTGTTGTT GTTGGAGACG TAGAGGTGAG TGAGGTGCTG 
GAAACTGATG GCAACATCCC TGATGTGCAT AATAAGTTTG ATCCAATTGG CCAAGGTGAA 
GGTGGTGAAG TTGAACTTGA ATCCGATAAA GCCACAGAGG AGGGAGGAGG AAAGTTGGTT 
AGTGAAGGAG ACTCTATGGT TGACTCCTCT GTGGTGGACT CTGTTGATGC TGATATCAAT 
GTTGCAGAGC CAGGGGTTGT GGTTGTCGGG GCGGCTAAGG AGGCTGTAAT TAAGGAAGAT 
GATAAAGATG ATGAGGTTGA CAAGACCATC TCAAATATTG AGGAACCTGA TGACCTGACT 
GCTGCATATG ATGGAAATTT TGAATTGGCT GTTAAGGAAA TATCAGAGGC TGCCAAAGTA 
GAGCCAGACG AACCAAAAGT AGGTGTGGAA GTAGAAGAAT TGCCTGTTTC TGAAAGTTTA 
AAAGTGGGTT CAGTTGATGC AGAAGAGGAT TCAATTCCTG CAGCTGAGTC ACAGTTTGAG 
GTTCGGAAAG TTGTCGAAGG GGACAGTGCT GAAGAGGATG AAAACAAGCT CCCAGTCGAA 
GACATTGTTT CTTCTCGTGA GTTTAGCTTT GGAGGCAAGG AAGTGGACCA GGAACCTTCT 
GGCGAAGGTG TTACCAGGGT TGATGGGTCT GAAAGTGAAG AAGAAACTGA AGAGATGATA 
TTTGGGAGTT CTGAAGCTGC AAAACAGTTC TTAGCAGAGT TGGAAAAGGC TTCTTCTGGT 
ATAGAGGCAC ATTCTGATGA AGCAAACATT TCTAACAATA TGTCAGATAG GATTGATGGC 
CAGATTGTCA CCGATTCTGA CGAGGATGTA GACACAGAGG ATGAAGGTGA GGAGAAAATG 
TTTGATACTG CAGCTTTGGC TGCGCTTTTG AAGGCAGCCA CTGGTGGTGG AAGTTCAGAA 
GGTGGCAATT TTACCATAAC ATCTCAGGAT GGCACGAAGC TTTTCTCTAT GGATCGACCT 
GCTGGTTTGA GTTCATCGTT AAGGCCCTTG AAGCCTGCAG CAGCTCCACG TGCAAACCGT 
TCCAACATCT TTTCCAATTC TAATGTCACA ATGGCAGATG AGACTGAGAT TAACTTGAGC 
GAGGAAGAGA AACAGAAATT AGAAAAGTTG CAAAGCCTCC GTGTGAAGTT TTTGCGGCTT 
TTGCAAAGGT TGGGTCATTC GGCAGAAGAT TCAATTGCAG CGCAGGTTCT TTACCGTCTT 
GCACTACTTG CTGGGAGACA AGCAGGGCAG TTATTCAGTC TTGATGCTGC AAAGAAGAAG 
GCTGTGGAGT CTGAGGCTGA GGGCAACGAA GAATTGATCT TCTCCCTAAA CATACTGGTC 
CTTGGAAAAG CCGGGGTGGG AAAAAGTGCT ACTATAAATT CCATTTTGGG AAACCAGATT 
GCGTCCATTG ATGCTTTTGG GCTCTCAACC ACTTCGGTTA GAGAAATTTC TGGAACGGTG 
AATGGTGTCA AGATTACCTT TATTGATACT CCGGGTTTGA AGTCTGCTGC GATGGATCAA 
AGTACAAATG CAAAAATGTT GTCCTCTGTA AAGAAGGTAA TGAAGAAGTG TCCCCCTGAT 
ATTGTACTAT ATGTAGATCG TCTTGACACC CAGACCAGGG ACTTGAACAA TTTGCCCTTG 
CTAAGGACGA TTACTGCTTC TCTTGGTACA TCCATATGGA AAAACGCTAT AGTGACATTG 
ACCCATGCGG CTTCTGCCCC TCCTGATGGC CCATCTGGCA CCCCATTGAG CTATGATGTG 
TTTGTAGCGC AGTGCTCACA TATTGTGCAA CAGTCTATAG GACAGGCTGT TGGAGATCTA 
CGCTTGATGA ATCCAAGTCT GATGAATCCA GTTTCACTTG TTGAGAATCA CCCCTTGTGT 
AGGAAGAACC GGGAGGGAGT GAAGGTTCTC CCGAATGGCC AAACTTGGAG ATCTCAGCTG 
TTGCTATTGT GTTACTCCCT GAAGGTTTTG TCAGAAACAA ATTCTCTATT GAGGCCTCAA 
GAACCATTGG ACCATCGTAA AGTATTTGGT TTCCGAGTTA GATCCCCGCC TCTCCCTTAC 
TTGTTGTCTT GGCTGTTGCA GTCCCGTGCA CATCCTAAGC TCCCTGGAGA CCAAGGTGGT 
GATAGTGTTG ACTCTGATAT CGAAATTGAT GATGTGTCTG ATTCTGAGCA GGAAGATGGG 
GAAGATGATG AGTATGACCA GCTGCCACCA TTTAAGCCTC TTCGAAAAAC CCAACTTGCA 
AAGCTCTCAA ATGAACAGAG AAAGGCATAT TTCGAGGAGT ATGATTACCG TGTAAAGCTC 
CTGCAGAAGA AGCAATGGAG AGAGGAATTA AAGAGGATGA AAGAAATGAA GAAAAACGGG 
AAGAAGCTGG GTGAAAGTGA GTTTGGTTAT CCGGGAGAAG AAGATGATCC AGAAAACGGG 
GCTCCAGCTG CAGTGCCAGT TCCATTACCC GATATGGTTT TGCCACCTTC ATTTGATAGT 
GATAACTCAG CTTACCGATA CCGATATCTG GAACCCACTT CACAACTCCT AACCAGGCCA 
GTGTTGGATA CCCATGGTTG GGACCATGAC TGTGGATATG ACGGCGTCAA TGCAGAACAC 
AGTCTTGCTC TAGCTAGCCG GTTCCCTGCC ACAGCTACTG TCCAAGTCAC CAAGGACAAG 
AAAGAGTTCA ACATTCATCT GGACTCCTCT GTGTCTGCTA AGCACGGGGA GAATGGATCC 
ACCATGGCAG GGTTCGATAT TCAGAATGTA GGCAAGCAGC TGGCATATGT GGTCAGAGGA 
GAAACCAAAT TCAAGAATTT GAGGAAGAAC AAGACAACTG TTGGAGGGTC AGTGACATTC 
TTGGGAGAGA ACATCGCCAC TGGGGTCAAA CTCGAGGACC AAATAGCACT GGGGAAAAGG 
TTGGTGCTTG TGGGCAGCAC TGGGACAATG CGATCACAGG GAGATTCGGC CTATGGTGCG 
AACCTCGAGG TCAGGCTTAG GGAAGCTGAT TTCCCAATTG GACAGGACCA ATCTTCTTTT 
GGGCTGTCTC TGGTAAAGTG GAGAGGCGAT TTAGCCCTTG GAGCCAATCT CCAATCTCAA 
GTCTCTGTTG GAAGGAACTC AAAGATTGCG CTTCGTGCAG GACTTAACAA CAAGATGAGC 
GGACAGATCA CAGTCAGAAC CAGCAGCTCG GATCAGTTGC AAATCGCTCT CACAGCCATT 
CTTCCAATTG CCATGTCCAT CTACAAGAGC ATTCGACCCG AAGCGACGAA CGACAAGTAC 
AGCATGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001328636.1
CDS	317..4828
Translation	MDSKSVTPEPTNPFYASSGQSGKTYASVVAAAAAAAADKEDGGAVSSAKELDSSSEAVSGNSDKVGADDLSDSEKEKPNLVGDGKVSDEVDGSLKEDSTTPEATPKPEVVSGETIGVDDVSSLSPKPEAVSDGVGVVEENKKVKEDVEDIKDDGESKIENGSVDVDVKQASTDGESESKVKDVEEEDVGTKKDDEGESELGGKVDVDDKSDNVIEEEGVELTDKGDVIVNSSPVESVHVDVAKPGVVVVGDAEGSEELKINADAETLEVANKFDQIGDDDSGEFEPVSDKAIEEVEEKFTSESDSIADSSKLESVDTSAVEPEVVAAESGSEPKDVEKANGLEKGMTYAEVIKAASAVADNGTKEEESVLGGIVDDAEEGVKLNNKGDFVVDSSAIEAVNVDVAKPGVVVVGDVEVSEVLETDGNIPDVHNKFDPIGQGEGGEVELESDKATEEGGGKLVSEGDSMVDSSVVDSVDADINVAEPGVVVVGAAKEAVIKEDDKDDEVDKTISNIEEPDDLTAAYDGNFELAVKEISEAAKVEPDEPKVGVEVEELPVSESLKVGSVDAEEDSIPAAESQFEVRKVVEGDSAEEDENKLPVEDIVSSREFSFGGKEVDQEPSGEGVTRVDGSESEEETEEMIFGSSEAAKQFLAELEKASSGIEAHSDEANISNNMSDRIDGQIVTDSDEDVDTEDEGEEKMFDTAALAALLKAATGGGSSEGGNFTITSQDGTKLFSMDRPAGLSSSLRPLKPAAAPRANRSNIFSNSNVTMADETEINLSEEEKQKLEKLQSLRVKFLRLLQRLGHSAEDSIAAQVLYRLALLAGRQAGQLFSLDAAKKKAVESEAEGNEELIFSLNILVLGKAGVGKSATINSILGNQIASIDAFGLSTTSVREISGTVNGVKITFIDTPGLKSAAMDQSTNAKMLSSVKKVMKKCPPDIVLYVDRLDTQTRDLNNLPLLRTITASLGTSIWKNAIVTLTHAASAPPDGPSGTPLSYDVFVAQCSHIVQQSIGQAVGDLRLMNPSLMNPVSLVENHPLCRKNREGVKVLPNGQTWRSQLLLLCYSLKVLSETNSLLRPQEPLDHRKVFGFRVRSPPLPYLLSWLLQSRAHPKLPGDQGGDSVDSDIEIDDVSDSEQEDGEDDEYDQLPPFKPLRKTQLAKLSNEQRKAYFEEYDYRVKLLQKKQWREELKRMKEMKKNGKKLGESEFGYPGEEDDPENGAPAAVPVPLPDMVLPPSFDSDNSAYRYRYLEPTSQLLTRPVLDTHGWDHDCGYDGVNAEHSLALASRFPATATVQVTKDKKEFNIHLDSSVSAKHGENGSTMAGFDIQNVGKQLAYVVRGETKFKNLRKNKTTVGGSVTFLGENIATGVKLEDQIALGKRLVLVGSTGTMRSQGDSAYGANLEVRLREADFPIGQDQSSFGLSLVKWRGDLALGANLQSQVSVGRNSKIALRAGLNNKMSGQITVRTSSSDQLQIALTAILPIAMSIYKSIRPEATNDKYSMY

Target ORF information:

RefSeq Version	NM_001340384.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 159 (TOC159), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001340384.1

ORF Insert Sequence:

ATGGACTCAA AGTCGGTTAC TCCAGAACCA ACCAACCCCT TCTACGCTTC TTCGGGGCAA 
TCAGGAAAAA CCTATGCTTC TGTTGTCGCC GCCGCTGCTG CTGCAGCCGC CGATAAGGAG 
GATGGTGGTG CTGTGAGTAG TGCCAAGGAG TTGGATTCCT CATCGGAGGC TGTGTCTGGT 
AATTCGGATA AGGTTGGAGC TGATGATTTA TCTGACTCCG AGAAGGAGAA GCCGAATTTG 
GTGGGTGATG GGAAGGTTTC CGACGAGGTG GATGGTTCTT TAAAGGAGGA TTCTACTACT 
CCTGAGGCTA CTCCGAAGCC TGAGGTGGTT TCTGGTGAGA CAATTGGTGT AGATGATGTT 
TCATCGTTAT CTCCGAAGCC GGAGGCTGTT TCTGATGGTG TAGGGGTTGT GGAGGAGAAT 
AAGAAGGTTA AGGAGGACGT GGAGGATATT AAAGACGATG GTGAGAGTAA GATTGAAAAT 
GGGAGTGTTG ATGTTGATGT GAAACAGGCT TCCACAGATG GGGAGAGTGA GAGTAAAGTG 
AAGGATGTGG AAGAAGAAGA TGTTGGAACA AAAAAAGATG ATGAGGGGGA GAGTGAGTTG 
GGTGGAAAAG TTGATGTTGA CGATAAAAGT GATAATGTTA TTGAAGAAGA GGGTGTGGAG 
TTAACTGATA AAGGAGACGT CATTGTGAAC TCGTCCCCTG TAGAATCAGT GCATGTTGAT 
GTGGCAAAAC CAGGGGTGGT TGTTGTAGGA GACGCAGAGG GAAGTGAGGA GTTGAAGATT 
AATGCTGATG CTGAAACTCT TGAAGTGGCT AATAAGTTTG ATCAAATTGG GGATGATGAT 
AGTGGTGAAT TTGAACCTGT GTCTGACAAG GCCATTGAGG AGGTAGAAGA AAAATTTACT 
AGCGAATCAG ACTCTATTGC AGACTCCTCT AAGTTAGAAT CTGTGGATAC CAGTGCTGTA 
GAGCCAGAGG TTGTAGCTGC CGAGAGTGGG AGTGAACCAA AGGATGTGGA AAAAGCCAAC 
GGATTGGAGA AGGGTATGAC TTATGCTGAA GTAATCAAGG CAGCTAGTGC AGTAGCTGAC 
AATGGAACCA AAGAGGAGGA GAGTGTGCTC GGTGGCATAG TCGATGATGC AGAAGAAGGT 
GTTAAGTTAA ACAACAAAGG AGACTTTGTG GTGGACTCAT CTGCTATTGA AGCTGTGAAT 
GTAGATGTGG CAAAGCCAGG GGTTGTTGTT GTTGGAGACG TAGAGGTGAG TGAGGTGCTG 
GAAACTGATG GCAACATCCC TGATGTGCAT AATAAGTTTG ATCCAATTGG CCAAGGTGAA 
GGTGGTGAAG TTGAACTTGA ATCCGATAAA GCCACAGAGG AGGGAGGAGG AAAGTTGGTT 
AGTGAAGGAG ACTCTATGGT TGACTCCTCT GTGGTGGACT CTGTTGATGC TGATATCAAT 
GTTGCAGAGC CAGGGGTTGT GGTTGTCGGG GCGGCTAAGG AGGCTGTAAT TAAGGAAGAT 
GATAAAGATG ATGAGGTTGA CAAGACCATC TCAAATATTG AGGAACCTGA TGACCTGACT 
GCTGCATATG ATGGAAATTT TGAATTGGCT GTTAAGGAAA TATCAGAGGC TGCCAAAGTA 
GAGCCAGACG AACCAAAAGT AGGTGTGGAA GTAGAAGAAT TGCCTGTTTC TGAAAGTTTA 
AAAGTGGGTT CAGTTGATGC AGAAGAGGAT TCAATTCCTG CAGCTGAGTC ACAGTTTGAG 
GTTCGGAAAG TTGTCGAAGG GGACAGTGCT GAAGAGGATG AAAACAAGCT CCCAGTCGAA 
GACATTGTTT CTTCTCGTGA GTTTAGCTTT GGAGGCAAGG AAGTGGACCA GGAACCTTCT 
GGCGAAGGTG TTACCAGGGT TGATGGGTCT GAAAGTGAAG AAGAAACTGA AGAGATGATA 
TTTGGGAGTT CTGAAGCTGC AAAACAGTTC TTAGCAGAGT TGGAAAAGGC TTCTTCTGGT 
ATAGAGGCAC ATTCTGATGA AGCAAACATT TCTAACAATA TGTCAGATAG GATTGATGGC 
CAGATTGTCA CCGATTCTGA CGAGGATGTA GACACAGAGG ATGAAGGTGA GGAGAAAATG 
TTTGATACTG CAGCTTTGGC TGCGCTTTTG AAGGCAGCCA CTGGTGGTGG AAGTTCAGAA 
GGTGGCAATT TTACCATAAC ATCTCAGGAT GGCACGAAGC TTTTCTCTAT GGATCGACCT 
GCTGGTTTGA GTTCATCGTT AAGGCCCTTG AAGCCTGCAG CAGCTCCACG TGCAAACCGT 
TCCAACATCT TTTCCAATTC TAATGTCACA ATGGCAGATG AGACTGAGAT TAACTTGAGC 
GAGGAAGAGA AACAGAAATT AGAAAAGTTG CAAAGCCTCC GTGTGAAGTT TTTGCGGCTT 
TTGCAAAGGT TGGGTCATTC GGCAGAAGAT TCAATTGCAG CGCAGGTTCT TTACCGTCTT 
GCACTACTTG CTGGGAGACA AGCAGGGCAG TTATTCAGTC TTGATGCTGC AAAGAAGAAG 
GCTGTGGAGT CTGAGGCTGA GGGCAACGAA GAATTGATCT TCTCCCTAAA CATACTGGTC 
CTTGGAAAAG CCGGGGTGGG AAAAAGTGCT ACTATAAATT CCATTTTGGG AAACCAGATT 
GCGTCCATTG ATGCTTTTGG GCTCTCAACC ACTTCGGTTA GAGAAATTTC TGGAACGGTG 
AATGGTGTCA AGATTACCTT TATTGATACT CCGGGTTTGA AGTCTGCTGC GATGGATCAA 
AGTACAAATG CAAAAATGTT GTCCTCTGTA AAGAAGGTAA TGAAGAAGTG TCCCCCTGAT 
ATTGTACTAT ATGTAGATCG TCTTGACACC CAGACCAGGG ACTTGAACAA TTTGCCCTTG 
CTAAGGACGA TTACTGCTTC TCTTGGTACA TCCATATGGA AAAACGCTAT AGTGACATTG 
ACCCATGCGG CTTCTGCCCC TCCTGATGGC CCATCTGGCA CCCCATTGAG CTATGATGTG 
TTTGTAGCGC AGTGCTCACA TATTGTGCAA CAGTCTATAG GACAGGCTGT TGGAGATCTA 
CGCTTGATGA ATCCAAGTCT GATGAATCCA GTTTCACTTG TTGAGAATCA CCCCTTGTGT 
AGGAAGAACC GGGAGGGAGT GAAGGTTCTC CCGAATGGCC AAACTTGGAG ATCTCAGCTG 
TTGCTATTGT GTTACTCCCT GAAGGTTTTG TCAGAAACAA ATTCTCTATT GAGGCCTCAA 
GAACCATTGG ACCATCGTAA AGTATTTGGT TTCCGAGTTA GATCCCCGCC TCTCCCTTAC 
TTGTTGTCTT GGCTGTTGCA GTCCCGTGCA CATCCTAAGC TCCCTGGAGA CCAAGGTGGT 
GATAGTGTTG ACTCTGATAT CGAAATTGAT GATGTGTCTG ATTCTGAGCA GGAAGATGGG 
GAAGATGATG AGTATGACCA GCTGCCACCA TTTAAGCCTC TTCGAAAAAC CCAACTTGCA 
AAGCTCTCAA ATGAACAGAG AAAGGCATAT TTCGAGGAGT ATGATTACCG TGTAAAGCTC 
CTGCAGAAGA AGCAATGGAG AGAGGAATTA AAGAGGATGA AAGAAATGAA GAAAAACGGG 
AAGAAGCTGG GTGAAAGTGA GTTTGGTTAT CCGGGAGAAG AAGATGATCC AGAAAACGGG 
GCTCCAGCTG CAGTGCCAGT TCCATTACCC GATATGGTTT TGCCACCTTC ATTTGATAGT 
GATAACTCAG CTTACCGATA CCGATATCTG GAACCCACTT CACAACTCCT AACCAGGCCA 
GTGTTGGATA CCCATGGTTG GGACCATGAC TGTGGATATG ACGGCGTCAA TGCAGAACAC 
AGTCTTGCTC TAGCTAGCCG GTTCCCTGCC ACAGCTACTG TCCAAGTCAC CAAGGACAAG 
AAAGAGTTCA ACATTCATCT GGACTCCTCT GTGTCTGCTA AGCACGGGGA GAATGGATCC 
ACCATGGCAG GGTTCGATAT TCAGAATGTA GGCAAGCAGC TGGCATATGT GGTCAGAGGA 
GAAACCAAAT TCAAGAATTT GAGGAAGAAC AAGACAACTG TTGGAGGGTC AGTGACATTC 
TTGGGAGAGA ACATCGCCAC TGGGGTCAAA CTCGAGGACC AAATAGCACT GGGGAAAAGG 
TTGGTGCTTG TGGGCAGCAC TGGGACAATG CGATCACAGG GAGATTCGGC CTATGGTGCG 
AACCTCGAGG TCAGGCTTAG GGAAGCTGAT TTCCCAATTG GACAGGACCA ATCTTCTTTT 
GGGCTGTCTC TGGTAAAGTG GAGAGGCGAT TTAGCCCTTG GAGCCAATCT CCAATCTCAA 
GTCTCTGTTG GAAGGAACTC AAAGATTGCG CTTCGTGCAG GACTTAACAA CAAGATGAGC 
GGACAGATCA CAGTCAGAAC CAGCAGCTCG GATCAGTTGC AAATCGCTCT CACAGCCATT 
CTTCCAATTG CCATGTCCAT CTACAAGAGC ATTCGACCCG AAGCGACGAA CGACAAGTAC 
AGCATGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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TOC159 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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