AK-HSDH II cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AK-HSDH II
Entrez Gene ID	827715
Full Name	aspartate kinase-homoserine dehydrogenase ii
Synonyms	AK-HSDH,ASPARTATE KINASE-HOMOSERINE DEHYDROGENASE,T16H5.70,T16H5_70,aspartate kinase-homoserine dehydrogenase ii
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_202847.3	NP_974576.1	aspartate kinase-homoserine dehydrogenase ii
NM_118091.3	NP_193706.2	aspartate kinase-homoserine dehydrogenase ii

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os08g0342400	NP_001061592.1
Arabidopsis thaliana (thale cress)	AK-HSDH II	NP_974576.1
Saccharomyces cerevisiae (baker's yeast)	HOM6	NP_012673.3
Magnaporthe oryzae (rice blast fungus)	MGG_11450	XP_003717922.1
Oryza sativa (rice)	Os09g0294000	NP_001062794.1
Kluyveromyces lactis	KLLA0B03872g	XP_451706.2
Schizosaccharomyces pombe (fission yeast)	SPBC776.03	NP_596318.1
Arabidopsis thaliana (thale cress)	AK-HSDH I	NP_174408.1
Eremothecium gossypii	AGOS_ABL080W	NP_982867.1
Neurospora crassa	NCU03935	XP_957574.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that mutations in the Asp kinases (AKs) and dual-functional Asp kinase-homoserine dehydrogenases (AK-HSDHs) result in reduced transcript levels and gene product activitiee and increases in the contents of Asp-derived amino acids.
Title: Analysis of Loss-of-Function Mutants in Aspartate Kinase and Homoserine Dehydrogenase Genes Points to Complexity in the Regulation of Aspartate-Derived Amino Acid Contents.

The following AK-HSDH II gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AK-HSDH II cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb39505	NM_118091.3 Latest version!	Arabidopsis thaliana aspartate kinase-homoserine dehydrogenase ii (AK-HSDH II), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb39506	NM_202847.3 Latest version!	Arabidopsis thaliana aspartate kinase-homoserine dehydrogenase ii (AK-HSDH II), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AK-HSDH II ( NM_118091.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_193706.2 Arabidopsis thaliana aspartate kinase-homoserine dehydrogenase ii (AK-HSDH II), mRNA.

Price & Availability↑

CloneID	OAb39505
Clone ID Related Accession (Same CDS sequence)	NM_118091.3
Accession Version	NM_118091.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	aspartate kinase-homoserine dehydrogenase ii
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Feb 17, 2004 this sequence version replaced NM_118091.2.

ATGGCGACTC TGAAGCCGTC ATTTACTGTT TCTCCGCCGA ATAGTAATCC GATTAGATTT 
GGAAGTTTTC CGCCGCAATG CTTTCTCCGT GTTCCGAAAC CGCGGCGACT TATATTGCCT 
AGGTTTCGGA AGACGACTGG TGGTGGCGGC GGCTTGATTC GATGTGAGCT TCCAGATTTT 
CATCTATCAG CAACAGCAAC TACTGTATCA GGTGTATCGA CGGTGAATTT AGTGGATCAA 
GTTCAGATTC CTAAAGGTGA AATGTGGAGT GTTCACAAGT TTGGTGGGAC TTGTGTGGGA 
AACTCTCAGA GGATCAGAAA TGTAGCAGAG GTTATAATCA ATGATAATTC CGAAAGAAAA 
CTTGTGGTTG TCTCGGCGAT GTCGAAGGTT ACGGACATGA TGTATGACTT AATCCGCAAG 
GCACAATCAC GAGATGATTC TTATTTATCC GCGTTGGAAG CTGTCTTGGA AAAGCATCGT 
TTAACAGCTC GTGACCTTCT CGATGGAGAT GATCTCGCTA GTTTCTTGTC ACATTTGCAT 
AATGATATTA GTAATCTTAA AGCAATGCTT CGTGCTATAT ACATAGCTGG CCATGCATCA 
GAGTCGTTTT CAGATTTTGT TGCAGGACAT GGGGAGCTTT GGTCTGCTCA GATGCTATCA 
TATGTTGTCA GAAAGACTGG GCTTGAGTGC AAGTGGATGG ATACTAGAGA CGTGCTCATT 
GTTAATCCCA CCAGCTCTAA TCAGGTTGAT CCTGATTTTG GTGAATCTGA GAAGAGACTC 
GATAAATGGT TCTCCTTAAA TCCGTCGAAA ATTATTATTG CGACTGGGTT TATTGCTAGC 
ACTCCGCAAA ATATTCCAAC AACTTTGAAA AGAGATGGGA GTGATTTCTC AGCAGCTATT 
ATGGGTGCTT TATTGAGAGC TCGTCAAGTA ACCATTTGGA CAGATGTTGA TGGTGTATAC 
AGTGCGGATC CTCGTAAAGT TAATGAGGCA GTGATACTCC AGACACTTTC TTATCAAGAG 
GCCTGGGAAA TGTCTTATTT TGGAGCAAAT GTGTTACATC CTCGCACCAT CATTCCTGTG 
ATGCGATATA ATATTCCGAT TGTGATTAGA AATATTTTCA ATCTCTCTGC ACCGGGAACA 
ATAATCTGTC AACCTCCTGA AGATGATTAT GACCTTAAAC TGACAACTCC TGTCAAAGGG 
TTTGCAACTA TTGACAATTT GGCCCTCATA AATGTTGAAG GTACTGGAAT GGCTGGTGTA 
CCCGGTACTG CAAGTGACAT TTTTGGCTGT GTAAAAGATG TTGGAGCTAA TGTGATTATG 
ATATCACAGG CTAGCAGTGA GCATTCTGTG TGCTTTGCTG TGCCTGAGAA GGAAGTAAAC 
GCAGTCTCTG AGGCATTGCG GTCGAGATTT AGTGAAGCTT TACAAGCGGG ACGTCTTTCT 
CAGATTGAGG TGATACCAAA CTGTAGCATC TTAGCTGCAG TCGGCCAGAA AATGGCTAGT 
ACACCTGGAG TTAGTTGTAC ACTTTTCAGT GCTTTGGCGA AGGCTAATAT TAATGTCCGA 
GCTATATCTC AAGGTTGTTC TGAGTACAAT GTTACTGTCG TTATTAAACG TGAAGATAGC 
GTTAAGGCGT TAAGAGCTGT ACACTCGAGG TTTTTCTTGT CAAGAACAAC ATTAGCAATG 
GGAATCGTAG GACCGGGCTT GATTGGTGCA ACATTACTTG ACCAGCTGCG GGATCAGGCT 
GCTGTTCTCA AACAAGAATT TAACATTGAT CTGCGTGTTT TGGGAATCAC TGGTTCAAAG 
AAGATGTTAT TGAGTGACAT TGGTATTGAT TTGTCGAGAT GGAGAGAACT TCTAAACGAG 
AAGGGAACAG AGGCGGATTT GGATAAATTC ACTCAACAAG TGCATGGAAA TCATTTTATC 
CCCAACTCTG TAGTGGTTGA TTGTACAGCA GACTCTGCTA TTGCAAGCCG TTACTATGAT 
TGGTTACGAA AGGGAATTCA TGTCATTACC CCAAATAAAA AGGCTAACTC AGGTCCCCTC 
GATCAGTACT TGAAACTGAG AGATCTTCAA AGGAAATCCT ACACTCATTA CTTCTACGAA 
GCCACTGTTG GAGCTGGTCT TCCAATTATC AGCACTTTAC GTGGTCTCCT TGAGACAGGA 
GATAAGATAC TACGCATAGA GGGCATTTGC AGTGGAACTT TGAGTTATCT ATTCAACAAT 
TTTGTTGGAG ATCGAAGTTT CAGCGAGGTT GTCACTGAAG CAAAGAACGC AGGTTTCACT 
GAGCCTGATC CAAGAGATGA TTTATCTGGA ACTGATGTTG CAAGGAAGGT GATTATCCTC 
GCTCGAGAAT CTGGACTGAA ATTGGACCTC GCTGATCTCC CCATTAGAAG TCTCGTACCA 
GAACCTCTAA AAGGATGCAC TTCTGTTGAA GAATTCATGG AGAAACTCCC ACAGTACGAT 
GGAGACCTAG CAAAAGAAAG GCTAGATGCT GAAAACTCTG GGGAAGTAAG ATTATTCACA 
ACAAACGTTT TCCCTTTCGA TCAATGTGAT CATATTTTAA CCATATATAT ATGTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_193706.2
CDS	178..2757
Translation	MATLKPSFTVSPPNSNPIRFGSFPPQCFLRVPKPRRLILPRFRKTTGGGGGLIRCELPDFHLSATATTVSGVSTVNLVDQVQIPKGEMWSVHKFGGTCVGNSQRIRNVAEVIINDNSERKLVVVSAMSKVTDMMYDLIRKAQSRDDSYLSALEAVLEKHRLTARDLLDGDDLASFLSHLHNDISNLKAMLRAIYIAGHASESFSDFVAGHGELWSAQMLSYVVRKTGLECKWMDTRDVLIVNPTSSNQVDPDFGESEKRLDKWFSLNPSKIIIATGFIASTPQNIPTTLKRDGSDFSAAIMGALLRARQVTIWTDVDGVYSADPRKVNEAVILQTLSYQEAWEMSYFGANVLHPRTIIPVMRYNIPIVIRNIFNLSAPGTIICQPPEDDYDLKLTTPVKGFATIDNLALINVEGTGMAGVPGTASDIFGCVKDVGANVIMISQASSEHSVCFAVPEKEVNAVSEALRSRFSEALQAGRLSQIEVIPNCSILAAVGQKMASTPGVSCTLFSALAKANINVRAISQGCSEYNVTVVIKREDSVKALRAVHSRFFLSRTTLAMGIVGPGLIGATLLDQLRDQAAVLKQEFNIDLRVLGITGSKKMLLSDIGIDLSRWRELLNEKGTEADLDKFTQQVHGNHFIPNSVVVDCTADSAIASRYYDWLRKGIHVITPNKKANSGPLDQYLKLRDLQRKSYTHYFYEATVGAGLPIISTLRGLLETGDKILRIEGICSGTLSYLFNNFVGDRSFSEVVTEAKNAGFTEPDPRDDLSGTDVARKVIILARESGLKLDLADLPIRSLVPEPLKGCTSVEEFMEKLPQYDGDLAKERLDAENSGEVRLFTTNVFPFDQCDHILTIYICM

Target ORF information:

RefSeq Version	NM_118091.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana aspartate kinase-homoserine dehydrogenase ii (AK-HSDH II), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118091.3

ORF Insert Sequence:

ATGGCGACTC TGAAGCCGTC ATTTACTGTT TCTCCGCCGA ATAGTAATCC GATTAGATTT 
GGAAGTTTTC CGCCGCAATG CTTTCTCCGT GTTCCGAAAC CGCGGCGACT TATATTGCCT 
AGGTTTCGGA AGACGACTGG TGGTGGCGGC GGCTTGATTC GATGTGAGCT TCCAGATTTT 
CATCTATCAG CAACAGCAAC TACTGTATCA GGTGTATCGA CGGTGAATTT AGTGGATCAA 
GTTCAGATTC CTAAAGGTGA AATGTGGAGT GTTCACAAGT TTGGTGGGAC TTGTGTGGGA 
AACTCTCAGA GGATCAGAAA TGTAGCAGAG GTTATAATCA ATGATAATTC CGAAAGAAAA 
CTTGTGGTTG TCTCGGCGAT GTCGAAGGTT ACGGACATGA TGTATGACTT AATCCGCAAG 
GCACAATCAC GAGATGATTC TTATTTATCC GCGTTGGAAG CTGTCTTGGA AAAGCATCGT 
TTAACAGCTC GTGACCTTCT CGATGGAGAT GATCTCGCTA GTTTCTTGTC ACATTTGCAT 
AATGATATTA GTAATCTTAA AGCAATGCTT CGTGCTATAT ACATAGCTGG CCATGCATCA 
GAGTCGTTTT CAGATTTTGT TGCAGGACAT GGGGAGCTTT GGTCTGCTCA GATGCTATCA 
TATGTTGTCA GAAAGACTGG GCTTGAGTGC AAGTGGATGG ATACTAGAGA CGTGCTCATT 
GTTAATCCCA CCAGCTCTAA TCAGGTTGAT CCTGATTTTG GTGAATCTGA GAAGAGACTC 
GATAAATGGT TCTCCTTAAA TCCGTCGAAA ATTATTATTG CGACTGGGTT TATTGCTAGC 
ACTCCGCAAA ATATTCCAAC AACTTTGAAA AGAGATGGGA GTGATTTCTC AGCAGCTATT 
ATGGGTGCTT TATTGAGAGC TCGTCAAGTA ACCATTTGGA CAGATGTTGA TGGTGTATAC 
AGTGCGGATC CTCGTAAAGT TAATGAGGCA GTGATACTCC AGACACTTTC TTATCAAGAG 
GCCTGGGAAA TGTCTTATTT TGGAGCAAAT GTGTTACATC CTCGCACCAT CATTCCTGTG 
ATGCGATATA ATATTCCGAT TGTGATTAGA AATATTTTCA ATCTCTCTGC ACCGGGAACA 
ATAATCTGTC AACCTCCTGA AGATGATTAT GACCTTAAAC TGACAACTCC TGTCAAAGGG 
TTTGCAACTA TTGACAATTT GGCCCTCATA AATGTTGAAG GTACTGGAAT GGCTGGTGTA 
CCCGGTACTG CAAGTGACAT TTTTGGCTGT GTAAAAGATG TTGGAGCTAA TGTGATTATG 
ATATCACAGG CTAGCAGTGA GCATTCTGTG TGCTTTGCTG TGCCTGAGAA GGAAGTAAAC 
GCAGTCTCTG AGGCATTGCG GTCGAGATTT AGTGAAGCTT TACAAGCGGG ACGTCTTTCT 
CAGATTGAGG TGATACCAAA CTGTAGCATC TTAGCTGCAG TCGGCCAGAA AATGGCTAGT 
ACACCTGGAG TTAGTTGTAC ACTTTTCAGT GCTTTGGCGA AGGCTAATAT TAATGTCCGA 
GCTATATCTC AAGGTTGTTC TGAGTACAAT GTTACTGTCG TTATTAAACG TGAAGATAGC 
GTTAAGGCGT TAAGAGCTGT ACACTCGAGG TTTTTCTTGT CAAGAACAAC ATTAGCAATG 
GGAATCGTAG GACCGGGCTT GATTGGTGCA ACATTACTTG ACCAGCTGCG GGATCAGGCT 
GCTGTTCTCA AACAAGAATT TAACATTGAT CTGCGTGTTT TGGGAATCAC TGGTTCAAAG 
AAGATGTTAT TGAGTGACAT TGGTATTGAT TTGTCGAGAT GGAGAGAACT TCTAAACGAG 
AAGGGAACAG AGGCGGATTT GGATAAATTC ACTCAACAAG TGCATGGAAA TCATTTTATC 
CCCAACTCTG TAGTGGTTGA TTGTACAGCA GACTCTGCTA TTGCAAGCCG TTACTATGAT 
TGGTTACGAA AGGGAATTCA TGTCATTACC CCAAATAAAA AGGCTAACTC AGGTCCCCTC 
GATCAGTACT TGAAACTGAG AGATCTTCAA AGGAAATCCT ACACTCATTA CTTCTACGAA 
GCCACTGTTG GAGCTGGTCT TCCAATTATC AGCACTTTAC GTGGTCTCCT TGAGACAGGA 
GATAAGATAC TACGCATAGA GGGCATTTGC AGTGGAACTT TGAGTTATCT ATTCAACAAT 
TTTGTTGGAG ATCGAAGTTT CAGCGAGGTT GTCACTGAAG CAAAGAACGC AGGTTTCACT 
GAGCCTGATC CAAGAGATGA TTTATCTGGA ACTGATGTTG CAAGGAAGGT GATTATCCTC 
GCTCGAGAAT CTGGACTGAA ATTGGACCTC GCTGATCTCC CCATTAGAAG TCTCGTACCA 
GAACCTCTAA AAGGATGCAC TTCTGTTGAA GAATTCATGG AGAAACTCCC ACAGTACGAT 
GGAGACCTAG CAAAAGAAAG GCTAGATGCT GAAAACTCTG GGGAAGTAAG ATTATTCACA 
ACAAACGTTT TCCCTTTCGA TCAATGTGAT CATATTTTAA CCATATATAT ATGTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AK-HSDH II ( NM_202847.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_974576.1 Arabidopsis thaliana aspartate kinase-homoserine dehydrogenase ii (AK-HSDH II), mRNA.

Price & Availability↑

CloneID	OAb39506
Clone ID Related Accession (Same CDS sequence)	NM_202847.3
Accession Version	NM_202847.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	aspartate kinase-homoserine dehydrogenase ii
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_202847.2.

ATGGCGACTC TGAAGCCGTC ATTTACTGTT TCTCCGCCGA ATAGTAATCC GATTAGATTT 
GGAAGTTTTC CGCCGCAATG CTTTCTCCGT GTTCCGAAAC CGCGGCGACT TATATTGCCT 
AGGTTTCGGA AGACGACTGG TGGTGGCGGC GGCTTGATTC GATGTGAGCT TCCAGATTTT 
CATCTATCAG CAACAGCAAC TACTGTATCA GGTGTATCGA CGGTGAATTT AGTGGATCAA 
GTTCAGATTC CTAAAGGTGA AATGTGGAGT GTTCACAAGT TTGGTGGGAC TTGTGTGGGA 
AACTCTCAGA GGATCAGAAA TGTAGCAGAG GTTATAATCA ATGATAATTC CGAAAGAAAA 
CTTGTGGTTG TCTCGGCGAT GTCGAAGGTT ACGGACATGA TGTATGACTT AATCCGCAAG 
GCACAATCAC GAGATGATTC TTATTTATCC GCGTTGGAAG CTGTCTTGGA AAAGCATCGT 
TTAACAGCTC GTGACCTTCT CGATGGAGAT GATCTCGCTA GTTTCTTGTC ACATTTGCAT 
AATGATATTA GTAATCTTAA AGCAATGCTT CGTGCTATAT ACATAGCTGG CCATGCATCA 
GAGTCGTTTT CAGATTTTGT TGCAGGACAT GGGGAGCTTT GGTCTGCTCA GATGCTATCA 
TATGTTGTCA GAAAGACTGG GCTTGAGTGC AAGTGGATGG ATACTAGAGA CGTGCTCATT 
GTTAATCCCA CCAGCTCTAA TCAGGTTGAT CCTGATTTTG GTGAATCTGA GAAGAGACTC 
GATAAATGGT TCTCCTTAAA TCCGTCGAAA ATTATTATTG CGACTGGGTT TATTGCTAGC 
ACTCCGCAAA ATATTCCAAC AACTTTGAAA AGAGATGGGA GTGATTTCTC AGCAGCTATT 
ATGGGTGCTT TATTGAGAGC TCGTCAAGTA ACCATTTGGA CAGATGTTGA TGGTGTATAC 
AGTGCGGATC CTCGTAAAGT TAATGAGGCA GTGATACTCC AGACACTTTC TTATCAAGAG 
GCCTGGGAAA TGTCTTATTT TGGAGCAAAT GTGTTACATC CTCGCACCAT CATTCCTGTG 
ATGCGATATA ATATTCCGAT TGTGATTAGA AATATTTTCA ATCTCTCTGC ACCGGGAACA 
ATAATCTGTC AACCTCCTGA AGATGATTAT GACCTTAAAC TGACAACTCC TGTCAAAGGG 
TTTGCAACTA TTGACAATTT GGCCCTCATA AATGTTGAAG GTACTGGAAT GGCTGGTGTA 
CCCGGTACTG CAAGTGACAT TTTTGGCTGT GTAAAAGATG TTGGAGCTAA TGTGATTATG 
ATATCACAGG CTAGCAGTGA GCATTCTGTG TGCTTTGCTG TGCCTGAGAA GGAAGTAAAC 
GCAGTCTCTG AGGCATTGCG GTCGAGATTT AGTGAAGCTT TACAAGCGGG ACGTCTTTCT 
CAGATTGAGG TGATACCAAA CTGTAGCATC TTAGCTGCAG TCGGCCAGAA AATGGCTAGT 
ACACCTGGAG TTAGTTGTAC ACTTTTCAGT GCTTTGGCGA AGGCTAATAT TAATGTCCGA 
GCTATATCTC AAGGTTGTTC TGAGTACAAT GTTACTGTCG TTATTAAACG TGAAGATAGC 
GTTAAGGCGT TAAGAGCTGT ACACTCGAGG TTTTTCTTGT CAAGAACAAC ATTAGCAATG 
GGAATCGTAG GACCGGGCTT GATTGGTGCA ACATTACTTG ACCAGCTGCG GGATCAGGCT 
GCTGTTCTCA AACAAGAATT TAACATTGAT CTGCGTGTTT TGGGAATCAC TGGTTCAAAG 
AAGATGTTAT TGAGTGACAT TGGTATTGAT TTGTCGAGAT GGAGAGAACT TCTAAACGAG 
AAGGGAACAG AGGCGGATTT GGATAAATTC ACTCAACAAG TGCATGGAAA TCATTTTATC 
CCCAACTCTG TAGTGGTTGA TTGTACAGCA GACTCTGCTA TTGCAAGCCG TTACTATGAT 
TGGTTACGAA AGGGAATTCA TGTCATTACC CCAAATAAAA AGGCTAACTC AGGTCCCCTC 
GATCAGTACT TGAAACTGAG AGATCTTCAA AGGAAATCCT ACACTCATTA CTTCTACGAA 
GCCACTGTTG GAGCTGGTCT TCCAATTATC AGCACTTTAC GTGGTCTCCT TGAGACAGGA 
GATAAGATAC TACGCATAGA GGGCATTTGC AGTGGAACTT TGAGTTATCT ATTCAACAAT 
TTTGTTGGAG ATCGAAGTTT CAGCGAGGTT GTCACTGAAG CAAAGAACGC AGGTTTCACT 
GAGCCTGATC CAAGAGATGA TTTATCTGGA ACTGATGTTG CAAGGAAGGT GATTATCCTC 
GCTCGAGAAT CTGGACTGAA ATTGGACCTC GCTGATCTCC CCATTAGAAG TCTCGTACCA 
GAACCTCTAA AAGGATGCAC TTCTGTTGAA GAATTCATGG AGAAACTCCC ACAGTACGAT 
GGAGACCTAG CAAAAGAAAG GCTAGATGCT GAAAACTCTG GGGAAGTTCT GAGATATGTT 
GGAGTGGTGG ACGCTGTTAA CCAAAAGGGA ACAGTTGAAC TTCGAAGATA CAAGAAAGAA 
CATCCATTTG CGCAGCTCGC AGGTTCAGAC AACATAATAG CCTTCACAAC GACAAGGTAC 
AAGGATCATC CACTTATAGT CCGAGGACCT GGAGCTGGTG CTCAAGTCAC GGCCGGTGGT 
ATATTCAGCG ACATACTAAG GCTTGCATCT TATCTCGGTG CACCGTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_974576.1
CDS	219..2969
Translation	MATLKPSFTVSPPNSNPIRFGSFPPQCFLRVPKPRRLILPRFRKTTGGGGGLIRCELPDFHLSATATTVSGVSTVNLVDQVQIPKGEMWSVHKFGGTCVGNSQRIRNVAEVIINDNSERKLVVVSAMSKVTDMMYDLIRKAQSRDDSYLSALEAVLEKHRLTARDLLDGDDLASFLSHLHNDISNLKAMLRAIYIAGHASESFSDFVAGHGELWSAQMLSYVVRKTGLECKWMDTRDVLIVNPTSSNQVDPDFGESEKRLDKWFSLNPSKIIIATGFIASTPQNIPTTLKRDGSDFSAAIMGALLRARQVTIWTDVDGVYSADPRKVNEAVILQTLSYQEAWEMSYFGANVLHPRTIIPVMRYNIPIVIRNIFNLSAPGTIICQPPEDDYDLKLTTPVKGFATIDNLALINVEGTGMAGVPGTASDIFGCVKDVGANVIMISQASSEHSVCFAVPEKEVNAVSEALRSRFSEALQAGRLSQIEVIPNCSILAAVGQKMASTPGVSCTLFSALAKANINVRAISQGCSEYNVTVVIKREDSVKALRAVHSRFFLSRTTLAMGIVGPGLIGATLLDQLRDQAAVLKQEFNIDLRVLGITGSKKMLLSDIGIDLSRWRELLNEKGTEADLDKFTQQVHGNHFIPNSVVVDCTADSAIASRYYDWLRKGIHVITPNKKANSGPLDQYLKLRDLQRKSYTHYFYEATVGAGLPIISTLRGLLETGDKILRIEGICSGTLSYLFNNFVGDRSFSEVVTEAKNAGFTEPDPRDDLSGTDVARKVIILARESGLKLDLADLPIRSLVPEPLKGCTSVEEFMEKLPQYDGDLAKERLDAENSGEVLRYVGVVDAVNQKGTVELRRYKKEHPFAQLAGSDNIIAFTTTRYKDHPLIVRGPGAGAQVTAGGIFSDILRLASYLGAPS

Target ORF information:

RefSeq Version	NM_202847.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana aspartate kinase-homoserine dehydrogenase ii (AK-HSDH II), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_202847.3

ORF Insert Sequence:

ATGGCGACTC TGAAGCCGTC ATTTACTGTT TCTCCGCCGA ATAGTAATCC GATTAGATTT 
GGAAGTTTTC CGCCGCAATG CTTTCTCCGT GTTCCGAAAC CGCGGCGACT TATATTGCCT 
AGGTTTCGGA AGACGACTGG TGGTGGCGGC GGCTTGATTC GATGTGAGCT TCCAGATTTT 
CATCTATCAG CAACAGCAAC TACTGTATCA GGTGTATCGA CGGTGAATTT AGTGGATCAA 
GTTCAGATTC CTAAAGGTGA AATGTGGAGT GTTCACAAGT TTGGTGGGAC TTGTGTGGGA 
AACTCTCAGA GGATCAGAAA TGTAGCAGAG GTTATAATCA ATGATAATTC CGAAAGAAAA 
CTTGTGGTTG TCTCGGCGAT GTCGAAGGTT ACGGACATGA TGTATGACTT AATCCGCAAG 
GCACAATCAC GAGATGATTC TTATTTATCC GCGTTGGAAG CTGTCTTGGA AAAGCATCGT 
TTAACAGCTC GTGACCTTCT CGATGGAGAT GATCTCGCTA GTTTCTTGTC ACATTTGCAT 
AATGATATTA GTAATCTTAA AGCAATGCTT CGTGCTATAT ACATAGCTGG CCATGCATCA 
GAGTCGTTTT CAGATTTTGT TGCAGGACAT GGGGAGCTTT GGTCTGCTCA GATGCTATCA 
TATGTTGTCA GAAAGACTGG GCTTGAGTGC AAGTGGATGG ATACTAGAGA CGTGCTCATT 
GTTAATCCCA CCAGCTCTAA TCAGGTTGAT CCTGATTTTG GTGAATCTGA GAAGAGACTC 
GATAAATGGT TCTCCTTAAA TCCGTCGAAA ATTATTATTG CGACTGGGTT TATTGCTAGC 
ACTCCGCAAA ATATTCCAAC AACTTTGAAA AGAGATGGGA GTGATTTCTC AGCAGCTATT 
ATGGGTGCTT TATTGAGAGC TCGTCAAGTA ACCATTTGGA CAGATGTTGA TGGTGTATAC 
AGTGCGGATC CTCGTAAAGT TAATGAGGCA GTGATACTCC AGACACTTTC TTATCAAGAG 
GCCTGGGAAA TGTCTTATTT TGGAGCAAAT GTGTTACATC CTCGCACCAT CATTCCTGTG 
ATGCGATATA ATATTCCGAT TGTGATTAGA AATATTTTCA ATCTCTCTGC ACCGGGAACA 
ATAATCTGTC AACCTCCTGA AGATGATTAT GACCTTAAAC TGACAACTCC TGTCAAAGGG 
TTTGCAACTA TTGACAATTT GGCCCTCATA AATGTTGAAG GTACTGGAAT GGCTGGTGTA 
CCCGGTACTG CAAGTGACAT TTTTGGCTGT GTAAAAGATG TTGGAGCTAA TGTGATTATG 
ATATCACAGG CTAGCAGTGA GCATTCTGTG TGCTTTGCTG TGCCTGAGAA GGAAGTAAAC 
GCAGTCTCTG AGGCATTGCG GTCGAGATTT AGTGAAGCTT TACAAGCGGG ACGTCTTTCT 
CAGATTGAGG TGATACCAAA CTGTAGCATC TTAGCTGCAG TCGGCCAGAA AATGGCTAGT 
ACACCTGGAG TTAGTTGTAC ACTTTTCAGT GCTTTGGCGA AGGCTAATAT TAATGTCCGA 
GCTATATCTC AAGGTTGTTC TGAGTACAAT GTTACTGTCG TTATTAAACG TGAAGATAGC 
GTTAAGGCGT TAAGAGCTGT ACACTCGAGG TTTTTCTTGT CAAGAACAAC ATTAGCAATG 
GGAATCGTAG GACCGGGCTT GATTGGTGCA ACATTACTTG ACCAGCTGCG GGATCAGGCT 
GCTGTTCTCA AACAAGAATT TAACATTGAT CTGCGTGTTT TGGGAATCAC TGGTTCAAAG 
AAGATGTTAT TGAGTGACAT TGGTATTGAT TTGTCGAGAT GGAGAGAACT TCTAAACGAG 
AAGGGAACAG AGGCGGATTT GGATAAATTC ACTCAACAAG TGCATGGAAA TCATTTTATC 
CCCAACTCTG TAGTGGTTGA TTGTACAGCA GACTCTGCTA TTGCAAGCCG TTACTATGAT 
TGGTTACGAA AGGGAATTCA TGTCATTACC CCAAATAAAA AGGCTAACTC AGGTCCCCTC 
GATCAGTACT TGAAACTGAG AGATCTTCAA AGGAAATCCT ACACTCATTA CTTCTACGAA 
GCCACTGTTG GAGCTGGTCT TCCAATTATC AGCACTTTAC GTGGTCTCCT TGAGACAGGA 
GATAAGATAC TACGCATAGA GGGCATTTGC AGTGGAACTT TGAGTTATCT ATTCAACAAT 
TTTGTTGGAG ATCGAAGTTT CAGCGAGGTT GTCACTGAAG CAAAGAACGC AGGTTTCACT 
GAGCCTGATC CAAGAGATGA TTTATCTGGA ACTGATGTTG CAAGGAAGGT GATTATCCTC 
GCTCGAGAAT CTGGACTGAA ATTGGACCTC GCTGATCTCC CCATTAGAAG TCTCGTACCA 
GAACCTCTAA AAGGATGCAC TTCTGTTGAA GAATTCATGG AGAAACTCCC ACAGTACGAT 
GGAGACCTAG CAAAAGAAAG GCTAGATGCT GAAAACTCTG GGGAAGTTCT GAGATATGTT 
GGAGTGGTGG ACGCTGTTAA CCAAAAGGGA ACAGTTGAAC TTCGAAGATA CAAGAAAGAA 
CATCCATTTG CGCAGCTCGC AGGTTCAGAC AACATAATAG CCTTCACAAC GACAAGGTAC 
AAGGATCATC CACTTATAGT CCGAGGACCT GGAGCTGGTG CTCAAGTCAC GGCCGGTGGT 
ATATTCAGCG ACATACTAAG GCTTGCATCT TATCTCGGTG CACCGTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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AK-HSDH II cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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