AT4G19010 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT4G19010
Entrez Gene ID	827639
Full Name	AMP-dependent synthetase and ligase family protein
Synonyms	F13C5.180,F13C5_180
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_118019.3	NP_193636.1	AMP-dependent synthetase and ligase family protein

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_07019	XP_003709777.1
Arabidopsis thaliana (thale cress)	AT4G19010	NP_193636.1
Neurospora crassa	NCU05851	XP_959842.1
Oryza sativa (rice)	Os08g0143300	NP_001175376.1
Oryza sativa (rice)	Os10g0578950	NP_001176290.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

At4g19010 is a coumarate CoA ligase that converts para-coumarate to coumarate-CoA in the peroxisomes. It functions in the biosynthesis of ubiquinone (Coenzyme Q).
Title: The Origin and Biosynthesis of the Benzenoid Moiety of Ubiquinone (Coenzyme Q) in Arabidopsis.

The following AT4G19010 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT4G19010 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb15351	NM_118019.3 Latest version!	Arabidopsis thaliana AMP-dependent synthetase and ligase family protein (AT4G19010), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT4G19010 ( NM_118019.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_193636.1 Arabidopsis thaliana AMP-dependent synthetase and ligase family protein (AT4G19010), mRNA.

Price & Availability↑

CloneID	OAb15351
Clone ID Related Accession (Same CDS sequence)	NM_118019.3
Accession Version	NM_118019.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	AMP-dependent synthetase and ligase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_118019.2.

ATGGCGGCGA CACATCTTCA CATACCGCCA AATCCTAAAA CCCAAACGTC TCACCAAAAC 
CCTCCCTTTT GGTTCTCTTC TAAAACAGGA ATCTACACCA GCAAATTCCC TTCCCTACAC 
CTCCCCGTCG ACCCAAATCT CGACGCCGTC TCCGCTCTTT TCTCACACAA ACACCACGGC 
GATACAGCGC TCATCGATTC CTTAACCGGG TTCTCAATAT CTCACACTGA GCTACAGATT 
ATGGTTCAAT CAATGGCGGC TGGGATCTAT CACGTTTTAG GTGTTCGTCA AGGTGACGTT 
GTATCACTCG TCTTGCCTAA TTCCGTCTAT TTTCCGATGA TTTTCCTCTC TTTGATCTCG 
CTTGGTGCTA TTGTTACTAC CATGAATCCT TCGAGTAGTT TAGGAGAGAT TAAGAAGCAA 
GTTAGTGAGT GTAGTGTTGG ATTAGCTTTT ACTTCTACTG AAAACGTTGA GAAGCTGAGT 
TCTTTGGGGG TTAGTGTGAT TAGTGTATCT GAAAGTTACG ATTTTGATTC GATTCGTATC 
GAAAACCCGA AGTTTTACTC CATTATGAAA GAAAGTTTTG GGTTTGTACC AAAACCGTTG 
ATTAAGCAAG ACGATGTAGC TGCAATTATG TATTCATCTG GAACAACTGG AGCTAGTAAA 
GGAGTTTTGT TAACTCATAG GAATTTGATA GCATCAATGG AGCTTTTTGT GAGGTTTGAA 
GCTTCTCAGT ACGAATATCC GGGATCGAGT AATGTTTATC TAGCAGCTTT GCCTTTGTGC 
CATATCTACG GGTTATCACT CTTTGTGATG GGATTGTTGT CTCTGGGATC AACTATTGTT 
GTTATGAAGA GGTTTGATGC TTCTGATGTT GTTAATGTAA TTGAAAGGTT TAAGATCACT 
CATTTCCCGG TTGTTCCACC TATGTTGATG GCATTGACAA AGAAGGCAAA AGGAGTTTGT 
GGTGAAGTGT TTAAAAGTTT GAAGCAAGTT TCTTCTGGAG CAGCTCCTTT GAGTAGGAAG 
TTCATTGAAG ATTTCCTTCA GACTCTTCCT CATGTCGATT TGATTCAGGG ATATGGAATG 
ACTGAATCAA CTGCAGTTGG AACACGGGGC TTCAACTCGG AAAAGCTTAG TAGGTATTCT 
TCTGTGGGAC TACTGGCTCC TAATATGCAA GCCAAGGTAG TAGATTGGAG CTCTGGTTCT 
TTCCTTCCAC CAGGAAACCG AGGAGAGCTC TGGATACAAG GTCCCGGTGT CATGAAAGGA 
TACTTGAATA ACCCAAAAGC AACTCAGATG AGTATAGTTG AAGATAGTTG GCTACGTACT 
GGGGATATTG CTTATTTTGA TGAAGATGGT TACTTGTTTA TTGTTGACCG TATTAAGGAG 
ATTATAAAGT ACAAAGGCTT TCAGATCGCT CCAGCAGATT TGGAGGCTGT TCTTGTTTCA 
CATCCCTTGA TTATCGACGC TGCAGTAACA GCTGCCCCAA ATGAAGAATG TGGAGAGATT 
CCGGTAGCAT TCGTTGTCCG GAGACAAGAA ACAACACTTT CAGAAGAAGA TGTAATAAGC 
TATGTAGCTT CTCAGGTTGC ACCCTACAGG AAGGTGAGGA AAGTGGTGAT GGTCAACTCT 
ATACCAAAAT CTCCAACTGG GAAGATATTG AGAAAGGAAC TAAAGAGAAT TCTTACAAAC 
TCTGTTTCTT CAAGACTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_193636.1
CDS	104..1804
Translation	MAATHLHIPPNPKTQTSHQNPPFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFSHKHHGDTALIDSLTGFSISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSSLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIENPKFYSIMKESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLMALTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRGFNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQMSIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKILRKELKRILTNSVSSRL

Target ORF information:

RefSeq Version	NM_118019.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana AMP-dependent synthetase and ligase family protein (AT4G19010), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118019.3

ORF Insert Sequence:

ATGGCGGCGA CACATCTTCA CATACCGCCA AATCCTAAAA CCCAAACGTC TCACCAAAAC 
CCTCCCTTTT GGTTCTCTTC TAAAACAGGA ATCTACACCA GCAAATTCCC TTCCCTACAC 
CTCCCCGTCG ACCCAAATCT CGACGCCGTC TCCGCTCTTT TCTCACACAA ACACCACGGC 
GATACAGCGC TCATCGATTC CTTAACCGGG TTCTCAATAT CTCACACTGA GCTACAGATT 
ATGGTTCAAT CAATGGCGGC TGGGATCTAT CACGTTTTAG GTGTTCGTCA AGGTGACGTT 
GTATCACTCG TCTTGCCTAA TTCCGTCTAT TTTCCGATGA TTTTCCTCTC TTTGATCTCG 
CTTGGTGCTA TTGTTACTAC CATGAATCCT TCGAGTAGTT TAGGAGAGAT TAAGAAGCAA 
GTTAGTGAGT GTAGTGTTGG ATTAGCTTTT ACTTCTACTG AAAACGTTGA GAAGCTGAGT 
TCTTTGGGGG TTAGTGTGAT TAGTGTATCT GAAAGTTACG ATTTTGATTC GATTCGTATC 
GAAAACCCGA AGTTTTACTC CATTATGAAA GAAAGTTTTG GGTTTGTACC AAAACCGTTG 
ATTAAGCAAG ACGATGTAGC TGCAATTATG TATTCATCTG GAACAACTGG AGCTAGTAAA 
GGAGTTTTGT TAACTCATAG GAATTTGATA GCATCAATGG AGCTTTTTGT GAGGTTTGAA 
GCTTCTCAGT ACGAATATCC GGGATCGAGT AATGTTTATC TAGCAGCTTT GCCTTTGTGC 
CATATCTACG GGTTATCACT CTTTGTGATG GGATTGTTGT CTCTGGGATC AACTATTGTT 
GTTATGAAGA GGTTTGATGC TTCTGATGTT GTTAATGTAA TTGAAAGGTT TAAGATCACT 
CATTTCCCGG TTGTTCCACC TATGTTGATG GCATTGACAA AGAAGGCAAA AGGAGTTTGT 
GGTGAAGTGT TTAAAAGTTT GAAGCAAGTT TCTTCTGGAG CAGCTCCTTT GAGTAGGAAG 
TTCATTGAAG ATTTCCTTCA GACTCTTCCT CATGTCGATT TGATTCAGGG ATATGGAATG 
ACTGAATCAA CTGCAGTTGG AACACGGGGC TTCAACTCGG AAAAGCTTAG TAGGTATTCT 
TCTGTGGGAC TACTGGCTCC TAATATGCAA GCCAAGGTAG TAGATTGGAG CTCTGGTTCT 
TTCCTTCCAC CAGGAAACCG AGGAGAGCTC TGGATACAAG GTCCCGGTGT CATGAAAGGA 
TACTTGAATA ACCCAAAAGC AACTCAGATG AGTATAGTTG AAGATAGTTG GCTACGTACT 
GGGGATATTG CTTATTTTGA TGAAGATGGT TACTTGTTTA TTGTTGACCG TATTAAGGAG 
ATTATAAAGT ACAAAGGCTT TCAGATCGCT CCAGCAGATT TGGAGGCTGT TCTTGTTTCA 
CATCCCTTGA TTATCGACGC TGCAGTAACA GCTGCCCCAA ATGAAGAATG TGGAGAGATT 
CCGGTAGCAT TCGTTGTCCG GAGACAAGAA ACAACACTTT CAGAAGAAGA TGTAATAAGC 
TATGTAGCTT CTCAGGTTGC ACCCTACAGG AAGGTGAGGA AAGTGGTGAT GGTCAACTCT 
ATACCAAAAT CTCCAACTGG GAAGATATTG AGAAAGGAAC TAAAGAGAAT TCTTACAAAC 
TCTGTTTCTT CAAGACTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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AT4G19010 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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