HAP2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	HAP2
Entrez Gene ID	826777
Full Name	hapless 2
Synonyms	GCS1,GENERATIVE CELL-SPECIFIC 1,HAPLESS 2,T5C23.150,T5C23_150
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_117241.2	NP_192909.2	hapless 2

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	HAP2	NP_192909.2
Oryza sativa (rice)	Os05g0269500	NP_001055054.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results provide data identifying the essential functional features of HAP2, a highly conserved sperm fusion protein.
Title: HAP2(GCS1)-dependent gamete fusion requires a positively charged carboxy-terminal domain.

The following HAP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HAP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb14655	NM_117241.2 Latest version!	Arabidopsis thaliana hapless 2 (HAP2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HAP2 ( NM_117241.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_192909.2 Arabidopsis thaliana hapless 2 (HAP2), mRNA.

Price & Availability↑

CloneID	OAb14655
Clone ID Related Accession (Same CDS sequence)	NM_117241.2
Accession Version	NM_117241.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	hapless 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Apr 18, 2007 this sequence version replaced NM_117241.1.

ATGGTGAACG CGATTTTAAT GGCTTGTATA CTCGCCGGAA TTTTCGTCGG AATGTTCAAC 
GAAGTCGACG GAATTCAGAT TTTATCGAAA TCAAAGCTCG AAAAGTGTGA GAAAACATCA 
GATTCTGGTA ATCTCAATTG TTCCACTAAG ATCGTCTTGA ATCTCGCTGT TCCCAGTGGA 
TCCAGTGGAG GAGAGGCTTC GATTGTAGCG GAGATCGTGG AGGTTGAAGA TAACTCGAGT 
AGTAATATGC AAACCGTACG GATTCCACCG GTTATTACAG TGAACAAATC TGCAGCATAT 
GCTCTCTATG ATCTTACTTA CATTAGGGAT GTACCTTATA AACCTCAAGA GTATCATGTC 
ACGACTCGAA AGTGTGAACA CGATGCTGGA CCGGATATTG TACAAATTTG TGAGAGGTTG 
AGAGATGAGA AGGGTAATGT TTTAGAGCAG ACTCAGCCGA TTTGTTGTCC ATGTGGACCG 
CAACGAAGAA TGCCTTCTTC TTGTGGAGAT ATTTTTGATA AGATGATTAA AGGGAAGGCA 
AATACCGCGC ATTGCCTTCG TTTTCCAGGT GACTGGTTCC ATGTTTTCGG GATTGGACAA 
CGGTCACTAG GATTTAGTGT GCGAGTTGAG TTGAAAACAG GAACTAGAGT TTCAGAAGTG 
ATCATTGGTC CTGAAAATAG GACAGCTACT GCAAATGATA ACTTTCTCAA AGTTAATCTC 
ATAGGAGACT TTGGTGGTTA CACAAGTATT CCTTCTTTCG AGGACTTCTA TCTTGTAATC 
CCAAGAGAGG CGGCTGAAGC GGGACAACCA GGGAGCTTAG GCGCAAATTA CTCAATGTGG 
ATGCTGCTTG AGAGAGTGAG ATTTACATTG GATGGCTTAG AATGTAATAA AATCGGTGTT 
GGTTATGAAG CTTTCAACAC CCAACCAAAT TTCTGCTCTT CTCCATATTG GAGTTGCTTG 
CATAATCAAC TATGGAACTT CCGTGAGTCA GATATAAACA GAATCGACCG ACACCAACTT 
CCTTTATATG GACTTGAAGG AAGATTTGAG AGGATCAATC AACATCCAAA CGCGGGGCCT 
CACTCGTTCT CAATTGGAGT CACGGAAACA CTCAACACAA ATCTAATGAT AGAACTAAGA 
GCTGATGATA TTGAATACGT TTTCCAGAGG AGTCCCGGTA AGATCATAAA CATCGCGATA 
CCGACATTCG AAGCACTTAC ACAATTCGGA GTTGCTGCAG TCATAATCAA GAATACTGGT 
GAAGTAGAAG CGTCTTATAG CTTAACGTTT GATTGCTCCA AAGGCGTGGC TTTTGTTGAG 
GAACAATTTT TCATTATAAA ACCGAAAGCG GTTACTACTC GGTCTTTTAA GCTGTACCCG 
ACGAAAGATC AGGCTGCAAA GTATATATGC ACAGCTATAC TCAAGGACTC TCAGTTCAGC 
GAAGTAGACC GAGCCGAGTG TCAATTTAGT ACAACGGCTA CCGTACTTGA CAATGGAACT 
CAGGTTACTA ATCCATTTCA AATTCCAGAG ACTCAACCTA AAGGTTTCTT TGATTCGATC 
AGGATACTAT GGACGAAAAT CATCAATGGT TTAGTCGATT TCATCACCGG AGATACTTGC 
AGAAACAAAT GCTCGAGTTT CTTCGACTTC AGTTGCCACA TTCAATACGT TTGCTTAAGC 
TGGATGGTTA TGTTTGGTCT TCTCCTCGCT CTCTTCCCAA TTACTTGTTT GCTTCTTTGG 
CTCTTGCACC AAAAGGGACT CTTTGATCCA TGCTACGACT GGTGGGAAGA TCATTTCGAT 
TTAGACCATC ATCGGAGATT ATTACCCTCC AGAGCAGACG TTGTTAACCG CCATCATCAC 
CATCACAAGC ATCGTCATCA CCATAATCAC CATCGTAGGA CACACCAGAG ACACAAACAT 
CATCATGGTC AAGACGACGA CGTATTGCAG AAGATGATGC TTGAACGTGA CCACAGCGAC 
TCTCACTACT ACCACCAACT TCATCGAGTC CATAAAGATA GCAAGCAGAA GCAGCGTAGG 
CGAGCCAAGC ACGGGATCGT CCTGCCACGT GATGTGCACG TGGAAAGACA GAGGAAACAA 
AGACTACGTG AGAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_192909.2
CDS	37..2154
Translation	MVNAILMACILAGIFVGMFNEVDGIQILSKSKLEKCEKTSDSGNLNCSTKIVLNLAVPSGSSGGEASIVAEIVEVEDNSSSNMQTVRIPPVITVNKSAAYALYDLTYIRDVPYKPQEYHVTTRKCEHDAGPDIVQICERLRDEKGNVLEQTQPICCPCGPQRRMPSSCGDIFDKMIKGKANTAHCLRFPGDWFHVFGIGQRSLGFSVRVELKTGTRVSEVIIGPENRTATANDNFLKVNLIGDFGGYTSIPSFEDFYLVIPREAAEAGQPGSLGANYSMWMLLERVRFTLDGLECNKIGVGYEAFNTQPNFCSSPYWSCLHNQLWNFRESDINRIDRHQLPLYGLEGRFERINQHPNAGPHSFSIGVTETLNTNLMIELRADDIEYVFQRSPGKIINIAIPTFEALTQFGVAAVIIKNTGEVEASYSLTFDCSKGVAFVEEQFFIIKPKAVTTRSFKLYPTKDQAAKYICTAILKDSQFSEVDRAECQFSTTATVLDNGTQVTNPFQIPETQPKGFFDSIRILWTKIINGLVDFITGDTCRNKCSSFFDFSCHIQYVCLSWMVMFGLLLALFPITCLLLWLLHQKGLFDPCYDWWEDHFDLDHHRRLLPSRADVVNRHHHHHKHRHHHNHHRRTHQRHKHHHGQDDDVLQKMMLERDHSDSHYYHQLHRVHKDSKQKQRRRAKHGIVLPRDVHVERQRKQRLRES

Target ORF information:

RefSeq Version	NM_117241.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana hapless 2 (HAP2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_117241.2

ORF Insert Sequence:

ATGGTGAACG CGATTTTAAT GGCTTGTATA CTCGCCGGAA TTTTCGTCGG AATGTTCAAC 
GAAGTCGACG GAATTCAGAT TTTATCGAAA TCAAAGCTCG AAAAGTGTGA GAAAACATCA 
GATTCTGGTA ATCTCAATTG TTCCACTAAG ATCGTCTTGA ATCTCGCTGT TCCCAGTGGA 
TCCAGTGGAG GAGAGGCTTC GATTGTAGCG GAGATCGTGG AGGTTGAAGA TAACTCGAGT 
AGTAATATGC AAACCGTACG GATTCCACCG GTTATTACAG TGAACAAATC TGCAGCATAT 
GCTCTCTATG ATCTTACTTA CATTAGGGAT GTACCTTATA AACCTCAAGA GTATCATGTC 
ACGACTCGAA AGTGTGAACA CGATGCTGGA CCGGATATTG TACAAATTTG TGAGAGGTTG 
AGAGATGAGA AGGGTAATGT TTTAGAGCAG ACTCAGCCGA TTTGTTGTCC ATGTGGACCG 
CAACGAAGAA TGCCTTCTTC TTGTGGAGAT ATTTTTGATA AGATGATTAA AGGGAAGGCA 
AATACCGCGC ATTGCCTTCG TTTTCCAGGT GACTGGTTCC ATGTTTTCGG GATTGGACAA 
CGGTCACTAG GATTTAGTGT GCGAGTTGAG TTGAAAACAG GAACTAGAGT TTCAGAAGTG 
ATCATTGGTC CTGAAAATAG GACAGCTACT GCAAATGATA ACTTTCTCAA AGTTAATCTC 
ATAGGAGACT TTGGTGGTTA CACAAGTATT CCTTCTTTCG AGGACTTCTA TCTTGTAATC 
CCAAGAGAGG CGGCTGAAGC GGGACAACCA GGGAGCTTAG GCGCAAATTA CTCAATGTGG 
ATGCTGCTTG AGAGAGTGAG ATTTACATTG GATGGCTTAG AATGTAATAA AATCGGTGTT 
GGTTATGAAG CTTTCAACAC CCAACCAAAT TTCTGCTCTT CTCCATATTG GAGTTGCTTG 
CATAATCAAC TATGGAACTT CCGTGAGTCA GATATAAACA GAATCGACCG ACACCAACTT 
CCTTTATATG GACTTGAAGG AAGATTTGAG AGGATCAATC AACATCCAAA CGCGGGGCCT 
CACTCGTTCT CAATTGGAGT CACGGAAACA CTCAACACAA ATCTAATGAT AGAACTAAGA 
GCTGATGATA TTGAATACGT TTTCCAGAGG AGTCCCGGTA AGATCATAAA CATCGCGATA 
CCGACATTCG AAGCACTTAC ACAATTCGGA GTTGCTGCAG TCATAATCAA GAATACTGGT 
GAAGTAGAAG CGTCTTATAG CTTAACGTTT GATTGCTCCA AAGGCGTGGC TTTTGTTGAG 
GAACAATTTT TCATTATAAA ACCGAAAGCG GTTACTACTC GGTCTTTTAA GCTGTACCCG 
ACGAAAGATC AGGCTGCAAA GTATATATGC ACAGCTATAC TCAAGGACTC TCAGTTCAGC 
GAAGTAGACC GAGCCGAGTG TCAATTTAGT ACAACGGCTA CCGTACTTGA CAATGGAACT 
CAGGTTACTA ATCCATTTCA AATTCCAGAG ACTCAACCTA AAGGTTTCTT TGATTCGATC 
AGGATACTAT GGACGAAAAT CATCAATGGT TTAGTCGATT TCATCACCGG AGATACTTGC 
AGAAACAAAT GCTCGAGTTT CTTCGACTTC AGTTGCCACA TTCAATACGT TTGCTTAAGC 
TGGATGGTTA TGTTTGGTCT TCTCCTCGCT CTCTTCCCAA TTACTTGTTT GCTTCTTTGG 
CTCTTGCACC AAAAGGGACT CTTTGATCCA TGCTACGACT GGTGGGAAGA TCATTTCGAT 
TTAGACCATC ATCGGAGATT ATTACCCTCC AGAGCAGACG TTGTTAACCG CCATCATCAC 
CATCACAAGC ATCGTCATCA CCATAATCAC CATCGTAGGA CACACCAGAG ACACAAACAT 
CATCATGGTC AAGACGACGA CGTATTGCAG AAGATGATGC TTGAACGTGA CCACAGCGAC 
TCTCACTACT ACCACCAACT TCATCGAGTC CATAAAGATA GCAAGCAGAA GCAGCGTAGG 
CGAGCCAAGC ACGGGATCGT CCTGCCACGT GATGTGCACG TGGAAAGACA GAGGAAACAA 
AGACTACGTG AGAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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HAP2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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