BB cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	BB
Entrez Gene ID	825528
Full Name	RING/U-box superfamily protein
Synonyms	BB2,BIG BROTHER,ENHANCER1 OF DA1,EOD1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_148885.3	NP_680148.1	RING/U-box superfamily protein
NM_001035845.2	NP_001030922.1	RING/U-box superfamily protein

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	BB	NP_680148.1
Oryza sativa (rice)	Os03g0173900	NP_001049123.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Big brother protein role in controlling leaf growth and senescence
Title: Forever Young: The Role of Ubiquitin Receptor DA1 and E3 Ligase BIG BROTHER in Controlling Leaf Growth and Development.

Genetic analyses show that DA2 functions synergistically with DA1 to regulate seed size, but does so independently of EOD1. Further results reveal that DA2 interacts physically with DA1 in vitro and in vivo.
Title: The ubiquitin receptor DA1 interacts with the E3 ubiquitin ligase DA2 to regulate seed and organ size in Arabidopsis.

Conserved cis-elements are important for regulation of BB expression.
Title: Expression of the central growth regulator BIG BROTHER is regulated by multiple cis-elements.

The E3 ubiquitin ligase BIG BROTHER controls arabidopsis organ size in a dosage-dependent manner.
Title: The E3 ubiquitin ligase BIG BROTHER controls arabidopsis organ size in a dosage-dependent manner.

The following BB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb14241	NM_001035845.2 Latest version!	Arabidopsis thaliana RING/U-box superfamily protein (BB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OAb14241	NM_148885.3 Latest version!	Arabidopsis thaliana RING/U-box superfamily protein (BB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BB ( NM_148885.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_680148.1 Arabidopsis thaliana RING/U-box superfamily protein (BB), mRNA.

Price & Availability↑

CloneID	OAb14241
Clone ID Related Accession (Same CDS sequence)	NM_148885.3 , NM_001035845.2
Accession Version	NM_148885.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	RING/U-box superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Apr 18, 2007 this sequence version replaced NM_148885.2.

ATGAATGGAG ATAATAGACC AGTGGAAGAT GCTCATTACA CGGAGACAGG TTTCCCTTAT 
GCTGCTACTG GAAGTTACAT GGACTTTTAT GGTGGTGCGG CTCAGGGGCC TCTTAACTAC 
GATCATGCCG CAACTATGCA TCCTCAGGAC AATCTGTACT GGACCATGAA TACCAATGCA 
TACAAGTTTG GGTTTTCAGG ATCAGATAAT GCTTCTTTCT ATGGTTCATA TGACATGAAC 
GATCATTTAT CGAGGATGTC CATAGGGAGA ACAAATTGGG ACTATCATCC CATGGTGAAC 
GTTGCTGATG ATCCTGAAAA CACAGTTGCA CGTTCCGTCC AAATCGGAGA CACAGATGAG 
CACTCTGAAG CTGAAGAATG CATTGCAAAT GAGCATGATC CCGACAGTCC TCAGGTATCC 
TGGCAAGATG ACATTGATCC TGATACAATG ACCTATGAGG AATTAGTAGA GCTGGGGGAA 
GCAGTAGGAA CAGAAAGCAG GGGGTTGTCT CAGGAACTCA TAGAAACGCT TCCCACTAAA 
AAGTATAAGT TTGGGAGCAT CTTCTCCAGG AAAAGAGCTG GGGAGAGGTG TGTGATATGC 
CAGCTCAAGT ACAAGATAGG GGAGAGGCAA ATGAATCTGC CGTGCAAGCA TGTGTATCAT 
TCTGAATGCA TTTCCAAATG GCTAAGCATC AACAAGGTTT GCCCGGTGTG TAACAGCGAG 
GTCTTTGGGG AGCCCAGCAT TCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_680148.1
CDS	254..1000
Translation	MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNAYKFGFSGSDNASFYGSYDMNDHLSRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDTDEHSEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEVFGEPSIH

Target ORF information:

RefSeq Version	NM_148885.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana RING/U-box superfamily protein (BB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_148885.3

ORF Insert Sequence:

ATGAATGGAG ATAATAGACC AGTGGAAGAT GCTCATTACA CGGAGACAGG TTTCCCTTAT 
GCTGCTACTG GAAGTTACAT GGACTTTTAT GGTGGTGCGG CTCAGGGGCC TCTTAACTAC 
GATCATGCCG CAACTATGCA TCCTCAGGAC AATCTGTACT GGACCATGAA TACCAATGCA 
TACAAGTTTG GGTTTTCAGG ATCAGATAAT GCTTCTTTCT ATGGTTCATA TGACATGAAC 
GATCATTTAT CGAGGATGTC CATAGGGAGA ACAAATTGGG ACTATCATCC CATGGTGAAC 
GTTGCTGATG ATCCTGAAAA CACAGTTGCA CGTTCCGTCC AAATCGGAGA CACAGATGAG 
CACTCTGAAG CTGAAGAATG CATTGCAAAT GAGCATGATC CCGACAGTCC TCAGGTATCC 
TGGCAAGATG ACATTGATCC TGATACAATG ACCTATGAGG AATTAGTAGA GCTGGGGGAA 
GCAGTAGGAA CAGAAAGCAG GGGGTTGTCT CAGGAACTCA TAGAAACGCT TCCCACTAAA 
AAGTATAAGT TTGGGAGCAT CTTCTCCAGG AAAAGAGCTG GGGAGAGGTG TGTGATATGC 
CAGCTCAAGT ACAAGATAGG GGAGAGGCAA ATGAATCTGC CGTGCAAGCA TGTGTATCAT 
TCTGAATGCA TTTCCAAATG GCTAAGCATC AACAAGGTTT GCCCGGTGTG TAACAGCGAG 
GTCTTTGGGG AGCCCAGCAT TCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BB ( NM_001035845.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001030922.1 Arabidopsis thaliana RING/U-box superfamily protein (BB), mRNA.

Price & Availability↑

CloneID	OAb14241
Clone ID Related Accession (Same CDS sequence)	NM_148885.3 , NM_001035845.2
Accession Version	NM_001035845.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	RING/U-box superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_001035845.1.

ATGAATGGAG ATAATAGACC AGTGGAAGAT GCTCATTACA CGGAGACAGG TTTCCCTTAT 
GCTGCTACTG GAAGTTACAT GGACTTTTAT GGTGGTGCGG CTCAGGGGCC TCTTAACTAC 
GATCATGCCG CAACTATGCA TCCTCAGGAC AATCTGTACT GGACCATGAA TACCAATGCA 
TACAAGTTTG GGTTTTCAGG ATCAGATAAT GCTTCTTTCT ATGGTTCATA TGACATGAAC 
GATCATTTAT CGAGGATGTC CATAGGGAGA ACAAATTGGG ACTATCATCC CATGGTGAAC 
GTTGCTGATG ATCCTGAAAA CACAGTTGCA CGTTCCGTCC AAATCGGAGA CACAGATGAG 
CACTCTGAAG CTGAAGAATG CATTGCAAAT GAGCATGATC CCGACAGTCC TCAGGTATCC 
TGGCAAGATG ACATTGATCC TGATACAATG ACCTATGAGG AATTAGTAGA GCTGGGGGAA 
GCAGTAGGAA CAGAAAGCAG GGGGTTGTCT CAGGAACTCA TAGAAACGCT TCCCACTAAA 
AAGTATAAGT TTGGGAGCAT CTTCTCCAGG AAAAGAGCTG GGGAGAGGTG TGTGATATGC 
CAGCTCAAGT ACAAGATAGG GGAGAGGCAA ATGAATCTGC CGTGCAAGCA TGTGTATCAT 
TCTGAATGCA TTTCCAAATG GCTAAGCATC AACAAGGTTT GCCCGGTGTG TAACAGCGAG 
GTCTTTGGGG AGCCCAGCAT TCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030922.1
CDS	530..1276
Translation	MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNAYKFGFSGSDNASFYGSYDMNDHLSRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDTDEHSEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEVFGEPSIH

Target ORF information:

RefSeq Version	NM_001035845.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana RING/U-box superfamily protein (BB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001035845.2

ORF Insert Sequence:

ATGAATGGAG ATAATAGACC AGTGGAAGAT GCTCATTACA CGGAGACAGG TTTCCCTTAT 
GCTGCTACTG GAAGTTACAT GGACTTTTAT GGTGGTGCGG CTCAGGGGCC TCTTAACTAC 
GATCATGCCG CAACTATGCA TCCTCAGGAC AATCTGTACT GGACCATGAA TACCAATGCA 
TACAAGTTTG GGTTTTCAGG ATCAGATAAT GCTTCTTTCT ATGGTTCATA TGACATGAAC 
GATCATTTAT CGAGGATGTC CATAGGGAGA ACAAATTGGG ACTATCATCC CATGGTGAAC 
GTTGCTGATG ATCCTGAAAA CACAGTTGCA CGTTCCGTCC AAATCGGAGA CACAGATGAG 
CACTCTGAAG CTGAAGAATG CATTGCAAAT GAGCATGATC CCGACAGTCC TCAGGTATCC 
TGGCAAGATG ACATTGATCC TGATACAATG ACCTATGAGG AATTAGTAGA GCTGGGGGAA 
GCAGTAGGAA CAGAAAGCAG GGGGTTGTCT CAGGAACTCA TAGAAACGCT TCCCACTAAA 
AAGTATAAGT TTGGGAGCAT CTTCTCCAGG AAAAGAGCTG GGGAGAGGTG TGTGATATGC 
CAGCTCAAGT ACAAGATAGG GGAGAGGCAA ATGAATCTGC CGTGCAAGCA TGTGTATCAT 
TCTGAATGCA TTTCCAAATG GCTAAGCATC AACAAGGTTT GCCCGGTGTG TAACAGCGAG 
GTCTTTGGGG AGCCCAGCAT TCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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BB cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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