GCN2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCN2
Entrez Gene ID	825110
Full Name	protein kinase family protein
Synonyms	ARABIDOPSIS THALIANA GENERAL CONTROL NON-REPRESSIBLE 2,ATGCN2,GENERAL CONTROL NON-REPRESSIBLE 2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001339969.1	NP_001326041.1	protein kinase family protein
NM_001203206.1	NP_001190135.1	protein kinase family protein
NM_001339971.1	NP_001326040.1	protein kinase family protein
NM_001339970.1	NP_001326042.1	protein kinase family protein
NM_115803.3	NP_191500.2	protein kinase family protein

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os04g0492600	NP_001053178.1
Arabidopsis thaliana (thale cress)	GCN2	NP_191500.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results points to an unknown GCN2-dependent factor involved in the cascade of events triggered by glyphosate in plants.
Title: Protein kinase GCN2 mediates responses to glyphosate in Arabidopsis.

AtGCN2 plays pivotal roles in various cellular processes essential for normal growth and development.
Title: Characterization of Arabidopsis thaliana GCN2 kinase roles in seed germination and plant development.

The in vitro assays demonstrate the binding of the C-terminus of AtGCN2 to uncharged tRNA molecules and the enzymatic activities of AtGCN2 on both eIF2alpha homologues in A. thaliana.
Title: The GCN2 homologue in Arabidopsis thaliana interacts with uncharged tRNA and uses Arabidopsis eIF2? molecules as direct substrates.

GCN2 is activated following wounding and exposure to hormones. GCN2 can phosphorylate eIF2alpha and is essential in amino acid deprivation conditions.
Title: Arabidopsis eIF2alpha kinase GCN2 is essential for growth in stress conditions and is activated by wounding.

Analysis of wild-type and GT8359 (null mutant for AtGCN2) plants infected with Turnip yellow mosaic virus or Turnip crinkle virus showed that AteIF2alpha was not phosphorylated.
Title: GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2alpha in Arabidopsis.

The following GCN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb38489	NM_001339971.1 Latest version!	Arabidopsis thaliana protein kinase family protein (GCN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAb38490	NM_001339970.1 Latest version!	Arabidopsis thaliana protein kinase family protein (GCN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAb13910	NM_001339969.1 Latest version!	Arabidopsis thaliana protein kinase family protein (GCN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OAb13910	NM_115803.3 Latest version!	Arabidopsis thaliana protein kinase family protein (GCN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OAb29946	NM_001203206.1 Latest version!	Arabidopsis thaliana protein kinase family protein (GCN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCN2 ( NM_001339971.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001326040.1 Arabidopsis thaliana protein kinase family protein (GCN2), mRNA.

Price & Availability↑

CloneID	OAb38489
Clone ID Related Accession (Same CDS sequence)	NM_001339971.1
Accession Version	NM_001339971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	protein kinase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGGGTCGCA GCAGTTCGAA GAAAAAGAAG AAACGAGGAG GTAGCGGAAG AAGGGGTCAG 
CTGAAAGACC ATGGATCTAA TGCTGATGAA GATAATGAAC TTCTATCTGA GGAGATCACT 
GCTCTCTCTG CAATATTTCA AGAGGACTGC AAAGTTGTTT CTGATTCGCG TTCGCCTCCG 
CAAATAGCAA TCAAGCTCAG GCCGTACTCA AAGGACATGG GATATGAAGA CACCGACATA 
TCTGCTATGC TTATAGTTAG GTGCTTACCA GGATATCCTT ACAAGTGCCC CAAGCTTCAG 
ATTACTCCAG AACAAGGGTT GACAACAGCT GATGCTGAGA AGCTTTTATC TCTTCTCGAG 
GACCAGGCAA ATTCCAATGC TCGTGAAGGT CGGGTTATGA TATTCAACCT GGTGGAGGCT 
GCTCAAGAGT TTTTATCAGA AATCATTCCG GAAAGTCATG ATGAGGAATC TGTTCCATGC 
TTGACTGCAC ATCGAAGCAC TCAGTTCATT GAGCAACCTA TGCTTTCAAA TATAGCAAAA 
TCCTGTTCTG GTGGACCTTT TGTGTATGGT TTTATAGACC TATTTAGTGG CTTGGAAGAT 
GCAAGAAATT GGAGTCTGAC TCCAGATGAA AATAGGGGAA TCGTATCTTC AGTACAATCT 
CACCCACTAG ACACTTCAAG AATTTTGCAT CAGAAGCCAG ACAAGAATCT GAAGCGATTT 
GAAGACCATG CTAAAGAAGA AGTTGCACTG CCTGCTCCCA TTGCCAAACT GAATACTGTT 
CAAGAGGAGA ATGTTGATGA TACAAGCATC TCTTCTTTTG ACTCAAGTAA ATCTACTGAC 
GATGTGGAAT CTGGATTATT CCAAAATGAG AAGAAGGAAT CAAATCTTCA AGATGATACA 
GCTGAAGATG ACAGCACTAA CTCCGAAAGT GAGTCGCTGG GGTCATGGTC TTCTGATTCC 
TTAGCTCAAG ATCAAGTGCC TCAGATTAGC AAGAAAGATC TGTTGATGGT CCATTTACTT 
CGAGTAGCTT GCACTTCCCG AGGACCTTTG GCTGATGCAT TACCTCAGAT AACTGATGAA 
CTGCATGAGC TTGGTATATT GTCTGAAGAA GTGTTGGATT TAGCTTCCAA ATCATCTCCA 
GACTTTAATA GAACCTTTGA ACATGCATTC AATCAAAACA TGGCCTCAAC CAGTGTTCCT 
CAGTTTTGGG AGCCACCTTC TGATTCTTGC GAGCCAAATG CATCACTCCC AAGCTCGCGA 
TATCTCAATG ATTTTGAAGA GTTGAAACCC CTTGGCCAAG GTGGTTTCGG CCACGTTGTG 
TTGTGCAAAA ATAAACTGGA TGGAAGACAA TATGCAGTGA AGAAAATTCG ACTGAAGGAC 
AAAGAGATAC CTGTCAACAG TCGGATAGTT CGAGAAGTAG CAACACTTTC CCGTTTGCAG 
CATCAGCATG TTGTACGTTA CTATCAGGCC TGGTTCGAAA CAGGAGTTGT TGATCCCTTT 
GCTGGCGCAA ATTGGGGATC AAAAACTGCA GGGAGTTCAA TGTTCAGCTA CTCAGGTGCA 
GTGTCAACTG AAATTCCTGA GCAGGACAAT AATCTTGAGT CGACTTATCT ATATATTCAA 
ATGGAATATT GTCCCAGGAC TCTCCGCCAG GTTTTTGAAT CATATAACCA CTTCGACAAA 
GACTTTGCAT GGCATTTAAT TCGCCAAATT GTGGAAGGCT TAGCTCATAT CCATGGACAA 
GGAATAATTC ATCGGGATTT TACACCTAAC AATATTTTCT TTGACGCTCG GAATGATATT 
AAAATTGGGG ATTTTGGTCT TGCAAAGTTC TTGAAGCTGG AACAGTTGGA TCAAGATGGG 
GGTTTCTCTA CGGATGTGGC TGGAAGCGGA GTCGATAGTA CTGGTCAAGC TGGTACTTAC 
TTTTACACAG CACCTGAAAT TGAGCAAGAT TGGCCTAAGA TTGATGAAAA GGCCGACATG 
TATAGCTTAG GGGTTGTGTT CTTTGAACTT TGGCATCCTT TTGGAACCGC CATGGAGAGA 
CACGTTATTT TAACTAACCT GAAGCTGAAA GGGGAGCTAC CTCTCAAATG GGTAAATGAA 
TTTCCCGAAC AGGCGTCTCT ACTGCGGCGT TTGATGTCTC CAAGTCCATC TGATCGTCCC 
TCTGCCACAG AACTTCTTAA GCATGCATTT CCTCCCAGAA TGGAATCTGA GTTACTGGAC 
AATATTCTAA GAATAATGCA AACTTCTGAA GATTCAAGTG TTTATGATAG AGTAGTAAGT 
GTGATATTTG ATGAAGAAGT ATTAGAGATG AAAAGCCATC AGTCTAGTAG ATCGAGACTC 
TGTGCAGATG ATAGTTATAT TCAATACACA GAGATAAATA CAGAGCTTCG TGATTATGTT 
GTTGAAATAA CAAAAGAAGT CTTTAGGCAG CATTGTGCGA AGCATCTAGA GGTCATACCA 
ATGCGCTTAC TTAGTGATTG CCCCCAGTTT AGCAGGAAAA CTGTAAAGCT TTTGACCAAT 
GGAGGAGATA TGCTTGAACT ATGCTATGAG CTACGACTGC CTTTTGTGCA TTGGATAAGC 
GTAAATCAGA AATCCTCATT CAAGCGATAT GAAATATCTC ATGTCTACAG GAGAGCAATT 
GGCCATTCTC CACCAAATCC GTGTCTTCAG GCGGACTTTG ACATTGTTGG AGGCACACTA 
TCCCTGACAG AGGCAGAAGT TCTCAAGGTG ATAGTAGACA TCACAACCCA CATCTTTCAT 
CGCGGATCTT GTGACATTCA TTTGAATCAT GGAGATTTGC TGGATGCGAT TTGGTCCTGG 
GCAGGAATTA AGGCAGAGCA TAGACGAAAG GTTGCAGAGC TTCTTTCCAT GATGGGATCC 
TTGCGTCCTC AGTCATCTGA GCGGAAGCTA AAATGGGTTT TCATAAGGCG TCAACTTCTT 
CAGGAGTTGA AGTTACCTGA AGCTGTTGTC AATAGACTGC AGACTGTTGC TTCAAGGTTT 
TGTGGAGATG CAGATCAAGC ACTTCCTCGT TTAAGAGGGG CTCTGCGTGC TGATAGACCT 
ACCCGCAAAG CACTCGATGA GTTGTCAAAC CTCTTAACCT ACCTGAGAGT CTGGAGGATA 
GAAGAGCATG TTCATATTGA TGTTCTGATG CCACCAACTG AAAGTTATCA CCGGAATTTG 
TTTTTTCAGG TTTTCTTAAC CAAAGAAAAT AGCTCTGGGA CATCTAATGA TGGCGTTTTA 
CTTGCTGTTG GTGGTCGTTA TGATTGGTTG GTGCAGGAAG TGTGTGATCG TGAACATAAA 
ATGAACCTCC CTGGTGCTGT TGGAGTTAGT CTTGCACTGG AGACAATATT TCAGCATCTT 
CCTATGGATC TAAGGCCTAT TAGAAATGAA GTCAGCACCA GTGTACTTGT TTGTTCAAGA 
GGAGGTGGTG GTTTACTGGT CCAGCGCATG GAACTAGTTG CGGAACTATG GGAAAAAAGT 
ATAAAGGCTG AGTTTGTTCC AACACCTGAT CCAAGTCTTA CTGAGCAGTA CGAATATGCA 
AATGAACATG AAATCAAATG TCTAGTGATC ATCACAGAGT CTGGAGTAGC TCAAAATCAA 
ATAGAGTTTG TAAAGGTAAT AAATACATCA TGTTCTGCTT ACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001326040.1
CDS	240..3887
Translation	MGRSSSKKKKKRGGSGRRGQLKDHGSNADEDNELLSEEITALSAIFQEDCKVVSDSRSPPQIAIKLRPYSKDMGYEDTDISAMLIVRCLPGYPYKCPKLQITPEQGLTTADAEKLLSLLEDQANSNAREGRVMIFNLVEAAQEFLSEIIPESHDEESVPCLTAHRSTQFIEQPMLSNIAKSCSGGPFVYGFIDLFSGLEDARNWSLTPDENRGIVSSVQSHPLDTSRILHQKPDKNLKRFEDHAKEEVALPAPIAKLNTVQEENVDDTSISSFDSSKSTDDVESGLFQNEKKESNLQDDTAEDDSTNSESESLGSWSSDSLAQDQVPQISKKDLLMVHLLRVACTSRGPLADALPQITDELHELGILSEEVLDLASKSSPDFNRTFEHAFNQNMASTSVPQFWEPPSDSCEPNASLPSSRYLNDFEELKPLGQGGFGHVVLCKNKLDGRQYAVKKIRLKDKEIPVNSRIVREVATLSRLQHQHVVRYYQAWFETGVVDPFAGANWGSKTAGSSMFSYSGAVSTEIPEQDNNLESTYLYIQMEYCPRTLRQVFESYNHFDKDFAWHLIRQIVEGLAHIHGQGIIHRDFTPNNIFFDARNDIKIGDFGLAKFLKLEQLDQDGGFSTDVAGSGVDSTGQAGTYFYTAPEIEQDWPKIDEKADMYSLGVVFFELWHPFGTAMERHVILTNLKLKGELPLKWVNEFPEQASLLRRLMSPSPSDRPSATELLKHAFPPRMESELLDNILRIMQTSEDSSVYDRVVSVIFDEEVLEMKSHQSSRSRLCADDSYIQYTEINTELRDYVVEITKEVFRQHCAKHLEVIPMRLLSDCPQFSRKTVKLLTNGGDMLELCYELRLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPCLQADFDIVGGTLSLTEAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRPQSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDADQALPRLRGALRADRPTRKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSGTSNDGVLLAVGGRYDWLVQEVCDREHKMNLPGAVGVSLALETIFQHLPMDLRPIRNEVSTSVLVCSRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVAQNQIEFVKVINTSCSAYY

Target ORF information:

RefSeq Version	NM_001339971.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana protein kinase family protein (GCN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001339971.1

ORF Insert Sequence:

ATGGGTCGCA GCAGTTCGAA GAAAAAGAAG AAACGAGGAG GTAGCGGAAG AAGGGGTCAG 
CTGAAAGACC ATGGATCTAA TGCTGATGAA GATAATGAAC TTCTATCTGA GGAGATCACT 
GCTCTCTCTG CAATATTTCA AGAGGACTGC AAAGTTGTTT CTGATTCGCG TTCGCCTCCG 
CAAATAGCAA TCAAGCTCAG GCCGTACTCA AAGGACATGG GATATGAAGA CACCGACATA 
TCTGCTATGC TTATAGTTAG GTGCTTACCA GGATATCCTT ACAAGTGCCC CAAGCTTCAG 
ATTACTCCAG AACAAGGGTT GACAACAGCT GATGCTGAGA AGCTTTTATC TCTTCTCGAG 
GACCAGGCAA ATTCCAATGC TCGTGAAGGT CGGGTTATGA TATTCAACCT GGTGGAGGCT 
GCTCAAGAGT TTTTATCAGA AATCATTCCG GAAAGTCATG ATGAGGAATC TGTTCCATGC 
TTGACTGCAC ATCGAAGCAC TCAGTTCATT GAGCAACCTA TGCTTTCAAA TATAGCAAAA 
TCCTGTTCTG GTGGACCTTT TGTGTATGGT TTTATAGACC TATTTAGTGG CTTGGAAGAT 
GCAAGAAATT GGAGTCTGAC TCCAGATGAA AATAGGGGAA TCGTATCTTC AGTACAATCT 
CACCCACTAG ACACTTCAAG AATTTTGCAT CAGAAGCCAG ACAAGAATCT GAAGCGATTT 
GAAGACCATG CTAAAGAAGA AGTTGCACTG CCTGCTCCCA TTGCCAAACT GAATACTGTT 
CAAGAGGAGA ATGTTGATGA TACAAGCATC TCTTCTTTTG ACTCAAGTAA ATCTACTGAC 
GATGTGGAAT CTGGATTATT CCAAAATGAG AAGAAGGAAT CAAATCTTCA AGATGATACA 
GCTGAAGATG ACAGCACTAA CTCCGAAAGT GAGTCGCTGG GGTCATGGTC TTCTGATTCC 
TTAGCTCAAG ATCAAGTGCC TCAGATTAGC AAGAAAGATC TGTTGATGGT CCATTTACTT 
CGAGTAGCTT GCACTTCCCG AGGACCTTTG GCTGATGCAT TACCTCAGAT AACTGATGAA 
CTGCATGAGC TTGGTATATT GTCTGAAGAA GTGTTGGATT TAGCTTCCAA ATCATCTCCA 
GACTTTAATA GAACCTTTGA ACATGCATTC AATCAAAACA TGGCCTCAAC CAGTGTTCCT 
CAGTTTTGGG AGCCACCTTC TGATTCTTGC GAGCCAAATG CATCACTCCC AAGCTCGCGA 
TATCTCAATG ATTTTGAAGA GTTGAAACCC CTTGGCCAAG GTGGTTTCGG CCACGTTGTG 
TTGTGCAAAA ATAAACTGGA TGGAAGACAA TATGCAGTGA AGAAAATTCG ACTGAAGGAC 
AAAGAGATAC CTGTCAACAG TCGGATAGTT CGAGAAGTAG CAACACTTTC CCGTTTGCAG 
CATCAGCATG TTGTACGTTA CTATCAGGCC TGGTTCGAAA CAGGAGTTGT TGATCCCTTT 
GCTGGCGCAA ATTGGGGATC AAAAACTGCA GGGAGTTCAA TGTTCAGCTA CTCAGGTGCA 
GTGTCAACTG AAATTCCTGA GCAGGACAAT AATCTTGAGT CGACTTATCT ATATATTCAA 
ATGGAATATT GTCCCAGGAC TCTCCGCCAG GTTTTTGAAT CATATAACCA CTTCGACAAA 
GACTTTGCAT GGCATTTAAT TCGCCAAATT GTGGAAGGCT TAGCTCATAT CCATGGACAA 
GGAATAATTC ATCGGGATTT TACACCTAAC AATATTTTCT TTGACGCTCG GAATGATATT 
AAAATTGGGG ATTTTGGTCT TGCAAAGTTC TTGAAGCTGG AACAGTTGGA TCAAGATGGG 
GGTTTCTCTA CGGATGTGGC TGGAAGCGGA GTCGATAGTA CTGGTCAAGC TGGTACTTAC 
TTTTACACAG CACCTGAAAT TGAGCAAGAT TGGCCTAAGA TTGATGAAAA GGCCGACATG 
TATAGCTTAG GGGTTGTGTT CTTTGAACTT TGGCATCCTT TTGGAACCGC CATGGAGAGA 
CACGTTATTT TAACTAACCT GAAGCTGAAA GGGGAGCTAC CTCTCAAATG GGTAAATGAA 
TTTCCCGAAC AGGCGTCTCT ACTGCGGCGT TTGATGTCTC CAAGTCCATC TGATCGTCCC 
TCTGCCACAG AACTTCTTAA GCATGCATTT CCTCCCAGAA TGGAATCTGA GTTACTGGAC 
AATATTCTAA GAATAATGCA AACTTCTGAA GATTCAAGTG TTTATGATAG AGTAGTAAGT 
GTGATATTTG ATGAAGAAGT ATTAGAGATG AAAAGCCATC AGTCTAGTAG ATCGAGACTC 
TGTGCAGATG ATAGTTATAT TCAATACACA GAGATAAATA CAGAGCTTCG TGATTATGTT 
GTTGAAATAA CAAAAGAAGT CTTTAGGCAG CATTGTGCGA AGCATCTAGA GGTCATACCA 
ATGCGCTTAC TTAGTGATTG CCCCCAGTTT AGCAGGAAAA CTGTAAAGCT TTTGACCAAT 
GGAGGAGATA TGCTTGAACT ATGCTATGAG CTACGACTGC CTTTTGTGCA TTGGATAAGC 
GTAAATCAGA AATCCTCATT CAAGCGATAT GAAATATCTC ATGTCTACAG GAGAGCAATT 
GGCCATTCTC CACCAAATCC GTGTCTTCAG GCGGACTTTG ACATTGTTGG AGGCACACTA 
TCCCTGACAG AGGCAGAAGT TCTCAAGGTG ATAGTAGACA TCACAACCCA CATCTTTCAT 
CGCGGATCTT GTGACATTCA TTTGAATCAT GGAGATTTGC TGGATGCGAT TTGGTCCTGG 
GCAGGAATTA AGGCAGAGCA TAGACGAAAG GTTGCAGAGC TTCTTTCCAT GATGGGATCC 
TTGCGTCCTC AGTCATCTGA GCGGAAGCTA AAATGGGTTT TCATAAGGCG TCAACTTCTT 
CAGGAGTTGA AGTTACCTGA AGCTGTTGTC AATAGACTGC AGACTGTTGC TTCAAGGTTT 
TGTGGAGATG CAGATCAAGC ACTTCCTCGT TTAAGAGGGG CTCTGCGTGC TGATAGACCT 
ACCCGCAAAG CACTCGATGA GTTGTCAAAC CTCTTAACCT ACCTGAGAGT CTGGAGGATA 
GAAGAGCATG TTCATATTGA TGTTCTGATG CCACCAACTG AAAGTTATCA CCGGAATTTG 
TTTTTTCAGG TTTTCTTAAC CAAAGAAAAT AGCTCTGGGA CATCTAATGA TGGCGTTTTA 
CTTGCTGTTG GTGGTCGTTA TGATTGGTTG GTGCAGGAAG TGTGTGATCG TGAACATAAA 
ATGAACCTCC CTGGTGCTGT TGGAGTTAGT CTTGCACTGG AGACAATATT TCAGCATCTT 
CCTATGGATC TAAGGCCTAT TAGAAATGAA GTCAGCACCA GTGTACTTGT TTGTTCAAGA 
GGAGGTGGTG GTTTACTGGT CCAGCGCATG GAACTAGTTG CGGAACTATG GGAAAAAAGT 
ATAAAGGCTG AGTTTGTTCC AACACCTGAT CCAAGTCTTA CTGAGCAGTA CGAATATGCA 
AATGAACATG AAATCAAATG TCTAGTGATC ATCACAGAGT CTGGAGTAGC TCAAAATCAA 
ATAGAGTTTG TAAAGGTAAT AAATACATCA TGTTCTGCTT ACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCN2 ( NM_001339970.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001326042.1 Arabidopsis thaliana protein kinase family protein (GCN2), mRNA.

Price & Availability↑

CloneID	OAb38490
Clone ID Related Accession (Same CDS sequence)	NM_001339970.1
Accession Version	NM_001339970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	protein kinase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGGGTCGCA GCAGTTCGAA GAAAAAGAAG AAACGAGGAG GTAGCGGAAG AAGGGGTCAG 
CTGAAAGACC ATGGATCTAA TGCTGATGAA GATAATGAAC TTCTATCTGA GGAGATCACT 
GCTCTCTCTG CAATATTTCA AGAGGACTGC AAAGTTGTTT CTGATTCGCG TTCGCCTCCG 
CAAATAGCAA TCAAGCTCAG GCCGTACTCA AAGGACATGG GATATGAAGA CACCGACATA 
TCTGCTATGC TTATAGTTAG GTGCTTACCA GGATATCCTT ACAAGTGCCC CAAGCTTCAG 
ATTACTCCAG AACAAGGGTT GACAACAGCT GATGCTGAGA AGCTTTTATC TCTTCTCGAG 
GACCAGGCAA ATTCCAATGC TCGTGAAGGT CGGGTTATGA TATTCAACCT GGTGGAGGCT 
GCTCAAGAGT TTTTATCAGA AATCATTCCG GAAAGTCATG ATGAGGAATC TGTTCCATGC 
TTGACTGCAC ATCGAAGCAC TCAGTTCATT GAGCAACCTA TGCTTTCAAA TATAGCAAAA 
TCCTGTTCTG GTGGACCTTT TGTGTATGGT TTTATAGACC TATTTAGTGG CTTGGAAGAT 
GCAAGAAATT GGAGTCTGAC TCCAGATGAA AATAGGGGAA TCGTATCTTC AGTACAATCT 
CACCCACTAG ACACTTCAAG AATTTTGCAT CAGAAGCCAG ACAAGAATCT GAAGCGATTT 
GAAGACCATG CTAAAGAAGA AGTTGCACTG CCTGCTCCCA TTGCCAAACT GAATACTGTT 
CAAGAGGAGA ATGTTGATGA TACAAGCATC TCTTCTTTTG ACTCAAGTAA ATCTACTGAC 
GATGTGGAAT CTGGATTATT CCAAAATGAG AAGAAGCAGG AATCAAATCT TCAAGATGAT 
ACAGCTGAAG ATGACAGCAC TAACTCCGAA AGTGAGTCGC TGGGGTCATG GTCTTCTGAT 
TCCTTAGCTC AAGATCAAGT GCCTCAGATT AGCAAGAAAG ATCTGTTGAT GGTCCATTTA 
CTTCGAGTAG CTTGCACTTC CCGAGGACCT TTGGCTGATG CATTACCTCA GATAACTGAT 
GAACTGCATG AGCTTGGTAT ATTGTCTGAA GAAGTGTTGG ATTTAGCTTC CAAATCATCT 
CCAGACTTTA ATAGAACCTT TGAACATGCA TTCAATCAAA ACATGGCCTC AACCAGTGTT 
CCTCAGTTTT GGGAGCCACC TTCTGATTCT TGCGAGCCAA ATGCATCACT CCCAAGCTCG 
CGATATCTCA ATGATTTTGA AGAGTTGAAA CCCCTTGGCC AAGGTGGTTT CGGCCACGTT 
GTGTTGTGCA AAAATAAACT GGATGGAAGA CAATATGCAG TGAAGAAAAT TCGACTGAAG 
GACAAAGAGA TACCTGTCAA CAGTCGGATA GTTCGAGAAG TAGCAACACT TTCCCGTTTG 
CAGCATCAGC ATGTTGTACG TTACTATCAG GCCTGGTTCG AAACAGGAGT TGTTGATCCC 
TTTGCTGGCG CAAATTGGGG ATCAAAAACT GCAGGGAGTT CAATGTTCAG CTACTCAGGT 
GCAGTGTCAA CTGAAATTCC TGAGCAGGAC AATAATCTTG AGTCGACTTA TCTATATATT 
CAAATGGAAT ATTGTCCCAG GACTCTCCGC CAGGTTTTTG AATCATATAA CCACTTCGAC 
AAAGACTTTG CATGGCATTT AATTCGCCAA ATTGTGGAAG GCTTAGCTCA TATCCATGGA 
CAAGGAATAA TTCATCGGGA TTTTACACCT AACAATATTT TCTTTGACGC TCGGAATGAT 
ATTAAAATTG GGGATTTTGG TCTTGCAAAG TTCTTGAAGC TGGAACAGTT GGATCAAGAT 
GGGGGTTTCT CTACGGATGT GGCTGGAAGC GGAGTCGATA GTACTGGTCA AGCTGGTACT 
TACTTTTACA CAGCACCTGA AATTGAGCAA GATTGGCCTA AGATTGATGA AAAGGCCGAC 
ATGTATAGCT TAGGGGTTGT GTTCTTTGAA CTTTGGCATC CTTTTGGAAC CGCCATGGAG 
AGACACGTTA TTTTAACTAA CCTGAAGCTG AAAGGGGAGC TACCTCTCAA ATGGGTAAAT 
GAATTTCCCG AACAGGCGTC TCTACTGCGG CGTTTGATGT CTCCAAGTCC ATCTGATCGT 
CCCTCTGCCA CAGAACTTCT TAAGCATGCA TTTCCTCCCA GAATGGAATC TGAGTTACTG 
GACAATATTC TAAGAATAAT GCAAACTTCT GAAGATTCAA GTGTTTATGA TAGAGTAGTA 
AGTGTGATAT TTGATGAAGA AGTATTAGAG ATGAAAAGCC ATCAGTCTAG TAGATCGAGA 
CTCTGTGCAG ATGATAGTTA TATTCAATAC ACAGAGATAA ATACAGAGCT TCGTGATTAT 
GTTGTTGAAA TAACAAAAGA AGTCTTTAGG CAGCATTGTG CGAAGCATCT AGAGGTCATA 
CCAATGCGCT TACTTAGTGA TTGCCCCCAG TTTAGCAGGA AAACTGTAAA GCTTTTGACC 
AATGGAGGAG ATATGCTTGA ACTATGCTAT GAGCTACGAC TGCCTTTTGT GCATTGGATA 
AGCGTAAATC AGAAATCCTC ATTCAAGCGA TATGAAATAT CTCATGTCTA CAGGAGAGCA 
ATTGGCCATT CTCCACCAAA TCCGTGTCTT CAGGCGGACT TTGACATTGT TGGAGGCACA 
CTATCCCTGA CAGAGGCAGA AGTTCTCAAG GTGATAGTAG ACATCACAAC CCACATCTTT 
CATCGCGGAT CTTGTGACAT TCATTTGAAT CATGGAGATT TGCTGGATGC GATTTGGTCC 
TGGGCAGGAA TTAAGGCAGA GCATAGACGA AAGGTTGCAG AGCTTCTTTC CATGATGGGA 
TCCTTGCGTC CTCAGTCATC TGAGCGGAAG CTAAAATGGG TTTTCATAAG GCGTCAACTT 
CTTCAGGAGT TGAAGTTACC TGAAGCTGTT GTCAATAGAC TGCAGACTGT TGCTTCAAGG 
TTTTGTGGAG ATGCAGATCA AGCACTTCCT CGTTTAAGAG GGGCTCTGCG TGCTGATAGA 
CCTACCCGCA AAGCACTCGA TGAGTTGTCA AACCTCTTAA CCTACCTGAG AGTCTGGAGG 
ATAGAAGAGC ATGTTCATAT TGATGTTCTG ATGCCACCAA CTGAAAGTTA TCACCGGAAT 
TTGTTTTTTC AGGTTTTCTT AACCAAAGAA AATAGCTCTG GGACATCTAA TGATGGCGTT 
TTACTTGCTG TTGGTGGTCG TTATGATTGG TTGGTGCAGG AAGTGTGTGA TCGTGAACAT 
AAAATGAACC TCCCTGGTGC TGTTGGAGTT AGTCTTGCAC TGGAGACAAT ATTTCAGCAT 
CTTCCTATGG ATCTAAGGCC TATTAGAAAT GAAGTCAGCA CCAGTGTACT TGTTTGTTCA 
AGAGGAGGTG GTGGTTTACT GGTCCAGCGC ATGGAACTAG TTGCGGAACT ATGGGAAAAA 
AGTATAAAGG CTGAGTTTGT TCCAACACCT GATCCAAGTC TTACTGAGCA GTACGAATAT 
GCAAATGAAC ATGAAATCAA ATGTCTAGTG ATCATCACAG AGTCTGGAGT AGCTCAAAAT 
CAAATAGAGT TTGTAAAGGT TCGTCACCTT GAACTGAAGA AGGAGAAAGT GGTAGGAAGA 
GAAGAACTTG TCAAATTTCT GCTGGATGCA ATGGCTGTTC AATTTAGAAA CCCCTCTGTT 
TGGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001326042.1
CDS	466..4194
Translation	MGRSSSKKKKKRGGSGRRGQLKDHGSNADEDNELLSEEITALSAIFQEDCKVVSDSRSPPQIAIKLRPYSKDMGYEDTDISAMLIVRCLPGYPYKCPKLQITPEQGLTTADAEKLLSLLEDQANSNAREGRVMIFNLVEAAQEFLSEIIPESHDEESVPCLTAHRSTQFIEQPMLSNIAKSCSGGPFVYGFIDLFSGLEDARNWSLTPDENRGIVSSVQSHPLDTSRILHQKPDKNLKRFEDHAKEEVALPAPIAKLNTVQEENVDDTSISSFDSSKSTDDVESGLFQNEKKQESNLQDDTAEDDSTNSESESLGSWSSDSLAQDQVPQISKKDLLMVHLLRVACTSRGPLADALPQITDELHELGILSEEVLDLASKSSPDFNRTFEHAFNQNMASTSVPQFWEPPSDSCEPNASLPSSRYLNDFEELKPLGQGGFGHVVLCKNKLDGRQYAVKKIRLKDKEIPVNSRIVREVATLSRLQHQHVVRYYQAWFETGVVDPFAGANWGSKTAGSSMFSYSGAVSTEIPEQDNNLESTYLYIQMEYCPRTLRQVFESYNHFDKDFAWHLIRQIVEGLAHIHGQGIIHRDFTPNNIFFDARNDIKIGDFGLAKFLKLEQLDQDGGFSTDVAGSGVDSTGQAGTYFYTAPEIEQDWPKIDEKADMYSLGVVFFELWHPFGTAMERHVILTNLKLKGELPLKWVNEFPEQASLLRRLMSPSPSDRPSATELLKHAFPPRMESELLDNILRIMQTSEDSSVYDRVVSVIFDEEVLEMKSHQSSRSRLCADDSYIQYTEINTELRDYVVEITKEVFRQHCAKHLEVIPMRLLSDCPQFSRKTVKLLTNGGDMLELCYELRLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPCLQADFDIVGGTLSLTEAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRPQSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDADQALPRLRGALRADRPTRKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSGTSNDGVLLAVGGRYDWLVQEVCDREHKMNLPGAVGVSLALETIFQHLPMDLRPIRNEVSTSVLVCSRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVAQNQIEFVKVRHLELKKEKVVGREELVKFLLDAMAVQFRNPSVWS

Target ORF information:

RefSeq Version	NM_001339970.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana protein kinase family protein (GCN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001339970.1

ORF Insert Sequence:

ATGGGTCGCA GCAGTTCGAA GAAAAAGAAG AAACGAGGAG GTAGCGGAAG AAGGGGTCAG 
CTGAAAGACC ATGGATCTAA TGCTGATGAA GATAATGAAC TTCTATCTGA GGAGATCACT 
GCTCTCTCTG CAATATTTCA AGAGGACTGC AAAGTTGTTT CTGATTCGCG TTCGCCTCCG 
CAAATAGCAA TCAAGCTCAG GCCGTACTCA AAGGACATGG GATATGAAGA CACCGACATA 
TCTGCTATGC TTATAGTTAG GTGCTTACCA GGATATCCTT ACAAGTGCCC CAAGCTTCAG 
ATTACTCCAG AACAAGGGTT GACAACAGCT GATGCTGAGA AGCTTTTATC TCTTCTCGAG 
GACCAGGCAA ATTCCAATGC TCGTGAAGGT CGGGTTATGA TATTCAACCT GGTGGAGGCT 
GCTCAAGAGT TTTTATCAGA AATCATTCCG GAAAGTCATG ATGAGGAATC TGTTCCATGC 
TTGACTGCAC ATCGAAGCAC TCAGTTCATT GAGCAACCTA TGCTTTCAAA TATAGCAAAA 
TCCTGTTCTG GTGGACCTTT TGTGTATGGT TTTATAGACC TATTTAGTGG CTTGGAAGAT 
GCAAGAAATT GGAGTCTGAC TCCAGATGAA AATAGGGGAA TCGTATCTTC AGTACAATCT 
CACCCACTAG ACACTTCAAG AATTTTGCAT CAGAAGCCAG ACAAGAATCT GAAGCGATTT 
GAAGACCATG CTAAAGAAGA AGTTGCACTG CCTGCTCCCA TTGCCAAACT GAATACTGTT 
CAAGAGGAGA ATGTTGATGA TACAAGCATC TCTTCTTTTG ACTCAAGTAA ATCTACTGAC 
GATGTGGAAT CTGGATTATT CCAAAATGAG AAGAAGCAGG AATCAAATCT TCAAGATGAT 
ACAGCTGAAG ATGACAGCAC TAACTCCGAA AGTGAGTCGC TGGGGTCATG GTCTTCTGAT 
TCCTTAGCTC AAGATCAAGT GCCTCAGATT AGCAAGAAAG ATCTGTTGAT GGTCCATTTA 
CTTCGAGTAG CTTGCACTTC CCGAGGACCT TTGGCTGATG CATTACCTCA GATAACTGAT 
GAACTGCATG AGCTTGGTAT ATTGTCTGAA GAAGTGTTGG ATTTAGCTTC CAAATCATCT 
CCAGACTTTA ATAGAACCTT TGAACATGCA TTCAATCAAA ACATGGCCTC AACCAGTGTT 
CCTCAGTTTT GGGAGCCACC TTCTGATTCT TGCGAGCCAA ATGCATCACT CCCAAGCTCG 
CGATATCTCA ATGATTTTGA AGAGTTGAAA CCCCTTGGCC AAGGTGGTTT CGGCCACGTT 
GTGTTGTGCA AAAATAAACT GGATGGAAGA CAATATGCAG TGAAGAAAAT TCGACTGAAG 
GACAAAGAGA TACCTGTCAA CAGTCGGATA GTTCGAGAAG TAGCAACACT TTCCCGTTTG 
CAGCATCAGC ATGTTGTACG TTACTATCAG GCCTGGTTCG AAACAGGAGT TGTTGATCCC 
TTTGCTGGCG CAAATTGGGG ATCAAAAACT GCAGGGAGTT CAATGTTCAG CTACTCAGGT 
GCAGTGTCAA CTGAAATTCC TGAGCAGGAC AATAATCTTG AGTCGACTTA TCTATATATT 
CAAATGGAAT ATTGTCCCAG GACTCTCCGC CAGGTTTTTG AATCATATAA CCACTTCGAC 
AAAGACTTTG CATGGCATTT AATTCGCCAA ATTGTGGAAG GCTTAGCTCA TATCCATGGA 
CAAGGAATAA TTCATCGGGA TTTTACACCT AACAATATTT TCTTTGACGC TCGGAATGAT 
ATTAAAATTG GGGATTTTGG TCTTGCAAAG TTCTTGAAGC TGGAACAGTT GGATCAAGAT 
GGGGGTTTCT CTACGGATGT GGCTGGAAGC GGAGTCGATA GTACTGGTCA AGCTGGTACT 
TACTTTTACA CAGCACCTGA AATTGAGCAA GATTGGCCTA AGATTGATGA AAAGGCCGAC 
ATGTATAGCT TAGGGGTTGT GTTCTTTGAA CTTTGGCATC CTTTTGGAAC CGCCATGGAG 
AGACACGTTA TTTTAACTAA CCTGAAGCTG AAAGGGGAGC TACCTCTCAA ATGGGTAAAT 
GAATTTCCCG AACAGGCGTC TCTACTGCGG CGTTTGATGT CTCCAAGTCC ATCTGATCGT 
CCCTCTGCCA CAGAACTTCT TAAGCATGCA TTTCCTCCCA GAATGGAATC TGAGTTACTG 
GACAATATTC TAAGAATAAT GCAAACTTCT GAAGATTCAA GTGTTTATGA TAGAGTAGTA 
AGTGTGATAT TTGATGAAGA AGTATTAGAG ATGAAAAGCC ATCAGTCTAG TAGATCGAGA 
CTCTGTGCAG ATGATAGTTA TATTCAATAC ACAGAGATAA ATACAGAGCT TCGTGATTAT 
GTTGTTGAAA TAACAAAAGA AGTCTTTAGG CAGCATTGTG CGAAGCATCT AGAGGTCATA 
CCAATGCGCT TACTTAGTGA TTGCCCCCAG TTTAGCAGGA AAACTGTAAA GCTTTTGACC 
AATGGAGGAG ATATGCTTGA ACTATGCTAT GAGCTACGAC TGCCTTTTGT GCATTGGATA 
AGCGTAAATC AGAAATCCTC ATTCAAGCGA TATGAAATAT CTCATGTCTA CAGGAGAGCA 
ATTGGCCATT CTCCACCAAA TCCGTGTCTT CAGGCGGACT TTGACATTGT TGGAGGCACA 
CTATCCCTGA CAGAGGCAGA AGTTCTCAAG GTGATAGTAG ACATCACAAC CCACATCTTT 
CATCGCGGAT CTTGTGACAT TCATTTGAAT CATGGAGATT TGCTGGATGC GATTTGGTCC 
TGGGCAGGAA TTAAGGCAGA GCATAGACGA AAGGTTGCAG AGCTTCTTTC CATGATGGGA 
TCCTTGCGTC CTCAGTCATC TGAGCGGAAG CTAAAATGGG TTTTCATAAG GCGTCAACTT 
CTTCAGGAGT TGAAGTTACC TGAAGCTGTT GTCAATAGAC TGCAGACTGT TGCTTCAAGG 
TTTTGTGGAG ATGCAGATCA AGCACTTCCT CGTTTAAGAG GGGCTCTGCG TGCTGATAGA 
CCTACCCGCA AAGCACTCGA TGAGTTGTCA AACCTCTTAA CCTACCTGAG AGTCTGGAGG 
ATAGAAGAGC ATGTTCATAT TGATGTTCTG ATGCCACCAA CTGAAAGTTA TCACCGGAAT 
TTGTTTTTTC AGGTTTTCTT AACCAAAGAA AATAGCTCTG GGACATCTAA TGATGGCGTT 
TTACTTGCTG TTGGTGGTCG TTATGATTGG TTGGTGCAGG AAGTGTGTGA TCGTGAACAT 
AAAATGAACC TCCCTGGTGC TGTTGGAGTT AGTCTTGCAC TGGAGACAAT ATTTCAGCAT 
CTTCCTATGG ATCTAAGGCC TATTAGAAAT GAAGTCAGCA CCAGTGTACT TGTTTGTTCA 
AGAGGAGGTG GTGGTTTACT GGTCCAGCGC ATGGAACTAG TTGCGGAACT ATGGGAAAAA 
AGTATAAAGG CTGAGTTTGT TCCAACACCT GATCCAAGTC TTACTGAGCA GTACGAATAT 
GCAAATGAAC ATGAAATCAA ATGTCTAGTG ATCATCACAG AGTCTGGAGT AGCTCAAAAT 
CAAATAGAGT TTGTAAAGGT TCGTCACCTT GAACTGAAGA AGGAGAAAGT GGTAGGAAGA 
GAAGAACTTG TCAAATTTCT GCTGGATGCA ATGGCTGTTC AATTTAGAAA CCCCTCTGTT 
TGGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCN2 ( NM_001339969.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001326041.1 Arabidopsis thaliana protein kinase family protein (GCN2), mRNA.

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CloneID	OAb13910
Clone ID Related Accession (Same CDS sequence)	NM_001339969.1 , NM_115803.3
Accession Version	NM_001339969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	protein kinase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGGGTCGCA GCAGTTCGAA GAAAAAGAAG AAACGAGGAG GTAGCGGAAG AAGGGGTCAG 
CTGAAAGACC ATGGATCTAA TGCTGATGAA GATAATGAAC TTCTATCTGA GGAGATCACT 
GCTCTCTCTG CAATATTTCA AGAGGACTGC AAAGTTGTTT CTGATTCGCG TTCGCCTCCG 
CAAATAGCAA TCAAGCTCAG GCCGTACTCA AAGGACATGG GATATGAAGA CACCGACATA 
TCTGCTATGC TTATAGTTAG GTGCTTACCA GGATATCCTT ACAAGTGCCC CAAGCTTCAG 
ATTACTCCAG AACAAGGGTT GACAACAGCT GATGCTGAGA AGCTTTTATC TCTTCTCGAG 
GACCAGGCAA ATTCCAATGC TCGTGAAGGT CGGGTTATGA TATTCAACCT GGTGGAGGCT 
GCTCAAGAGT TTTTATCAGA AATCATTCCG GAAAGTCATG ATGAGGAATC TGTTCCATGC 
TTGACTGCAC ATCGAAGCAC TCAGTTCATT GAGCAACCTA TGCTTTCAAA TATAGCAAAA 
TCCTGTTCTG GTGGACCTTT TGTGTATGGT TTTATAGACC TATTTAGTGG CTTGGAAGAT 
GCAAGAAATT GGAGTCTGAC TCCAGATGAA AATAGGGGAA TCGTATCTTC AGTACAATCT 
CACCCACTAG ACACTTCAAG AATTTTGCAT CAGAAGCCAG ACAAGAATCT GAAGCGATTT 
GAAGACCATG CTAAAGAAGA AGTTGCACTG CCTGCTCCCA TTGCCAAACT GAATACTGTT 
CAAGAGGAGA ATGTTGATGA TACAAGCATC TCTTCTTTTG ACTCAAGTAA ATCTACTGAC 
GATGTGGAAT CTGGATTATT CCAAAATGAG AAGAAGGAAT CAAATCTTCA AGATGATACA 
GCTGAAGATG ACAGCACTAA CTCCGAAAGT GAGTCGCTGG GGTCATGGTC TTCTGATTCC 
TTAGCTCAAG ATCAAGTGCC TCAGATTAGC AAGAAAGATC TGTTGATGGT CCATTTACTT 
CGAGTAGCTT GCACTTCCCG AGGACCTTTG GCTGATGCAT TACCTCAGAT AACTGATGAA 
CTGCATGAGC TTGGTATATT GTCTGAAGAA GTGTTGGATT TAGCTTCCAA ATCATCTCCA 
GACTTTAATA GAACCTTTGA ACATGCATTC AATCAAAACA TGGCCTCAAC CAGTGTTCCT 
CAGTTTTGGG AGCCACCTTC TGATTCTTGC GAGCCAAATG CATCACTCCC AAGCTCGCGA 
TATCTCAATG ATTTTGAAGA GTTGAAACCC CTTGGCCAAG GTGGTTTCGG CCACGTTGTG 
TTGTGCAAAA ATAAACTGGA TGGAAGACAA TATGCAGTGA AGAAAATTCG ACTGAAGGAC 
AAAGAGATAC CTGTCAACAG TCGGATAGTT CGAGAAGTAG CAACACTTTC CCGTTTGCAG 
CATCAGCATG TTGTACGTTA CTATCAGGCC TGGTTCGAAA CAGGAGTTGT TGATCCCTTT 
GCTGGCGCAA ATTGGGGATC AAAAACTGCA GGGAGTTCAA TGTTCAGCTA CTCAGGTGCA 
GTGTCAACTG AAATTCCTGA GCAGGACAAT AATCTTGAGT CGACTTATCT ATATATTCAA 
ATGGAATATT GTCCCAGGAC TCTCCGCCAG GTTTTTGAAT CATATAACCA CTTCGACAAA 
GACTTTGCAT GGCATTTAAT TCGCCAAATT GTGGAAGGCT TAGCTCATAT CCATGGACAA 
GGAATAATTC ATCGGGATTT TACACCTAAC AATATTTTCT TTGACGCTCG GAATGATATT 
AAAATTGGGG ATTTTGGTCT TGCAAAGTTC TTGAAGCTGG AACAGTTGGA TCAAGATGGG 
GGTTTCTCTA CGGATGTGGC TGGAAGCGGA GTCGATAGTA CTGGTCAAGC TGGTACTTAC 
TTTTACACAG CACCTGAAAT TGAGCAAGAT TGGCCTAAGA TTGATGAAAA GGCCGACATG 
TATAGCTTAG GGGTTGTGTT CTTTGAACTT TGGCATCCTT TTGGAACCGC CATGGAGAGA 
CACGTTATTT TAACTAACCT GAAGCTGAAA GGGGAGCTAC CTCTCAAATG GGTAAATGAA 
TTTCCCGAAC AGGCGTCTCT ACTGCGGCGT TTGATGTCTC CAAGTCCATC TGATCGTCCC 
TCTGCCACAG AACTTCTTAA GCATGCATTT CCTCCCAGAA TGGAATCTGA GTTACTGGAC 
AATATTCTAA GAATAATGCA AACTTCTGAA GATTCAAGTG TTTATGATAG AGTAGTAAGT 
GTGATATTTG ATGAAGAAGT ATTAGAGATG AAAAGCCATC AGTCTAGTAG ATCGAGACTC 
TGTGCAGATG ATAGTTATAT TCAATACACA GAGATAAATA CAGAGCTTCG TGATTATGTT 
GTTGAAATAA CAAAAGAAGT CTTTAGGCAG CATTGTGCGA AGCATCTAGA GGTCATACCA 
ATGCGCTTAC TTAGTGATTG CCCCCAGTTT AGCAGGAAAA CTGTAAAGCT TTTGACCAAT 
GGAGGAGATA TGCTTGAACT ATGCTATGAG CTACGACTGC CTTTTGTGCA TTGGATAAGC 
GTAAATCAGA AATCCTCATT CAAGCGATAT GAAATATCTC ATGTCTACAG GAGAGCAATT 
GGCCATTCTC CACCAAATCC GTGTCTTCAG GCGGACTTTG ACATTGTTGG AGGCACACTA 
TCCCTGACAG AGGCAGAAGT TCTCAAGGTG ATAGTAGACA TCACAACCCA CATCTTTCAT 
CGCGGATCTT GTGACATTCA TTTGAATCAT GGAGATTTGC TGGATGCGAT TTGGTCCTGG 
GCAGGAATTA AGGCAGAGCA TAGACGAAAG GTTGCAGAGC TTCTTTCCAT GATGGGATCC 
TTGCGTCCTC AGTCATCTGA GCGGAAGCTA AAATGGGTTT TCATAAGGCG TCAACTTCTT 
CAGGAGTTGA AGTTACCTGA AGCTGTTGTC AATAGACTGC AGACTGTTGC TTCAAGGTTT 
TGTGGAGATG CAGATCAAGC ACTTCCTCGT TTAAGAGGGG CTCTGCGTGC TGATAGACCT 
ACCCGCAAAG CACTCGATGA GTTGTCAAAC CTCTTAACCT ACCTGAGAGT CTGGAGGATA 
GAAGAGCATG TTCATATTGA TGTTCTGATG CCACCAACTG AAAGTTATCA CCGGAATTTG 
TTTTTTCAGG TTTTCTTAAC CAAAGAAAAT AGCTCTGGGA CATCTAATGA TGGCGTTTTA 
CTTGCTGTTG GTGGTCGTTA TGATTGGTTG GTGCAGGAAG TGTGTGATCG TGAACATAAA 
ATGAACCTCC CTGGTGCTGT TGGAGTTAGT CTTGCACTGG AGACAATATT TCAGCATCTT 
CCTATGGATC TAAGGCCTAT TAGAAATGAA GTCAGCACCA GTGTACTTGT TTGTTCAAGA 
GGAGGTGGTG GTTTACTGGT CCAGCGCATG GAACTAGTTG CGGAACTATG GGAAAAAAGT 
ATAAAGGCTG AGTTTGTTCC AACACCTGAT CCAAGTCTTA CTGAGCAGTA CGAATATGCA 
AATGAACATG AAATCAAATG TCTAGTGATC ATCACAGAGT CTGGAGTAGC TCAAAATCAA 
ATAGAGTTTG TAAAGGTTCG TCACCTTGAA CTGAAGAAGG AGAAAGTGGT AGGAAGAGAA 
GAACTTGTCA AATTTCTGCT GGATGCAATG GCTGTTCAAT TTAGAAACCC CTCTGTTTGG 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001326041.1
CDS	1327..5052
Translation	MGRSSSKKKKKRGGSGRRGQLKDHGSNADEDNELLSEEITALSAIFQEDCKVVSDSRSPPQIAIKLRPYSKDMGYEDTDISAMLIVRCLPGYPYKCPKLQITPEQGLTTADAEKLLSLLEDQANSNAREGRVMIFNLVEAAQEFLSEIIPESHDEESVPCLTAHRSTQFIEQPMLSNIAKSCSGGPFVYGFIDLFSGLEDARNWSLTPDENRGIVSSVQSHPLDTSRILHQKPDKNLKRFEDHAKEEVALPAPIAKLNTVQEENVDDTSISSFDSSKSTDDVESGLFQNEKKESNLQDDTAEDDSTNSESESLGSWSSDSLAQDQVPQISKKDLLMVHLLRVACTSRGPLADALPQITDELHELGILSEEVLDLASKSSPDFNRTFEHAFNQNMASTSVPQFWEPPSDSCEPNASLPSSRYLNDFEELKPLGQGGFGHVVLCKNKLDGRQYAVKKIRLKDKEIPVNSRIVREVATLSRLQHQHVVRYYQAWFETGVVDPFAGANWGSKTAGSSMFSYSGAVSTEIPEQDNNLESTYLYIQMEYCPRTLRQVFESYNHFDKDFAWHLIRQIVEGLAHIHGQGIIHRDFTPNNIFFDARNDIKIGDFGLAKFLKLEQLDQDGGFSTDVAGSGVDSTGQAGTYFYTAPEIEQDWPKIDEKADMYSLGVVFFELWHPFGTAMERHVILTNLKLKGELPLKWVNEFPEQASLLRRLMSPSPSDRPSATELLKHAFPPRMESELLDNILRIMQTSEDSSVYDRVVSVIFDEEVLEMKSHQSSRSRLCADDSYIQYTEINTELRDYVVEITKEVFRQHCAKHLEVIPMRLLSDCPQFSRKTVKLLTNGGDMLELCYELRLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPCLQADFDIVGGTLSLTEAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRPQSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDADQALPRLRGALRADRPTRKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSGTSNDGVLLAVGGRYDWLVQEVCDREHKMNLPGAVGVSLALETIFQHLPMDLRPIRNEVSTSVLVCSRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVAQNQIEFVKVRHLELKKEKVVGREELVKFLLDAMAVQFRNPSVWS

Target ORF information:

RefSeq Version	NM_001339969.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana protein kinase family protein (GCN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001339969.1

ORF Insert Sequence:

ATGGGTCGCA GCAGTTCGAA GAAAAAGAAG AAACGAGGAG GTAGCGGAAG AAGGGGTCAG 
CTGAAAGACC ATGGATCTAA TGCTGATGAA GATAATGAAC TTCTATCTGA GGAGATCACT 
GCTCTCTCTG CAATATTTCA AGAGGACTGC AAAGTTGTTT CTGATTCGCG TTCGCCTCCG 
CAAATAGCAA TCAAGCTCAG GCCGTACTCA AAGGACATGG GATATGAAGA CACCGACATA 
TCTGCTATGC TTATAGTTAG GTGCTTACCA GGATATCCTT ACAAGTGCCC CAAGCTTCAG 
ATTACTCCAG AACAAGGGTT GACAACAGCT GATGCTGAGA AGCTTTTATC TCTTCTCGAG 
GACCAGGCAA ATTCCAATGC TCGTGAAGGT CGGGTTATGA TATTCAACCT GGTGGAGGCT 
GCTCAAGAGT TTTTATCAGA AATCATTCCG GAAAGTCATG ATGAGGAATC TGTTCCATGC 
TTGACTGCAC ATCGAAGCAC TCAGTTCATT GAGCAACCTA TGCTTTCAAA TATAGCAAAA 
TCCTGTTCTG GTGGACCTTT TGTGTATGGT TTTATAGACC TATTTAGTGG CTTGGAAGAT 
GCAAGAAATT GGAGTCTGAC TCCAGATGAA AATAGGGGAA TCGTATCTTC AGTACAATCT 
CACCCACTAG ACACTTCAAG AATTTTGCAT CAGAAGCCAG ACAAGAATCT GAAGCGATTT 
GAAGACCATG CTAAAGAAGA AGTTGCACTG CCTGCTCCCA TTGCCAAACT GAATACTGTT 
CAAGAGGAGA ATGTTGATGA TACAAGCATC TCTTCTTTTG ACTCAAGTAA ATCTACTGAC 
GATGTGGAAT CTGGATTATT CCAAAATGAG AAGAAGGAAT CAAATCTTCA AGATGATACA 
GCTGAAGATG ACAGCACTAA CTCCGAAAGT GAGTCGCTGG GGTCATGGTC TTCTGATTCC 
TTAGCTCAAG ATCAAGTGCC TCAGATTAGC AAGAAAGATC TGTTGATGGT CCATTTACTT 
CGAGTAGCTT GCACTTCCCG AGGACCTTTG GCTGATGCAT TACCTCAGAT AACTGATGAA 
CTGCATGAGC TTGGTATATT GTCTGAAGAA GTGTTGGATT TAGCTTCCAA ATCATCTCCA 
GACTTTAATA GAACCTTTGA ACATGCATTC AATCAAAACA TGGCCTCAAC CAGTGTTCCT 
CAGTTTTGGG AGCCACCTTC TGATTCTTGC GAGCCAAATG CATCACTCCC AAGCTCGCGA 
TATCTCAATG ATTTTGAAGA GTTGAAACCC CTTGGCCAAG GTGGTTTCGG CCACGTTGTG 
TTGTGCAAAA ATAAACTGGA TGGAAGACAA TATGCAGTGA AGAAAATTCG ACTGAAGGAC 
AAAGAGATAC CTGTCAACAG TCGGATAGTT CGAGAAGTAG CAACACTTTC CCGTTTGCAG 
CATCAGCATG TTGTACGTTA CTATCAGGCC TGGTTCGAAA CAGGAGTTGT TGATCCCTTT 
GCTGGCGCAA ATTGGGGATC AAAAACTGCA GGGAGTTCAA TGTTCAGCTA CTCAGGTGCA 
GTGTCAACTG AAATTCCTGA GCAGGACAAT AATCTTGAGT CGACTTATCT ATATATTCAA 
ATGGAATATT GTCCCAGGAC TCTCCGCCAG GTTTTTGAAT CATATAACCA CTTCGACAAA 
GACTTTGCAT GGCATTTAAT TCGCCAAATT GTGGAAGGCT TAGCTCATAT CCATGGACAA 
GGAATAATTC ATCGGGATTT TACACCTAAC AATATTTTCT TTGACGCTCG GAATGATATT 
AAAATTGGGG ATTTTGGTCT TGCAAAGTTC TTGAAGCTGG AACAGTTGGA TCAAGATGGG 
GGTTTCTCTA CGGATGTGGC TGGAAGCGGA GTCGATAGTA CTGGTCAAGC TGGTACTTAC 
TTTTACACAG CACCTGAAAT TGAGCAAGAT TGGCCTAAGA TTGATGAAAA GGCCGACATG 
TATAGCTTAG GGGTTGTGTT CTTTGAACTT TGGCATCCTT TTGGAACCGC CATGGAGAGA 
CACGTTATTT TAACTAACCT GAAGCTGAAA GGGGAGCTAC CTCTCAAATG GGTAAATGAA 
TTTCCCGAAC AGGCGTCTCT ACTGCGGCGT TTGATGTCTC CAAGTCCATC TGATCGTCCC 
TCTGCCACAG AACTTCTTAA GCATGCATTT CCTCCCAGAA TGGAATCTGA GTTACTGGAC 
AATATTCTAA GAATAATGCA AACTTCTGAA GATTCAAGTG TTTATGATAG AGTAGTAAGT 
GTGATATTTG ATGAAGAAGT ATTAGAGATG AAAAGCCATC AGTCTAGTAG ATCGAGACTC 
TGTGCAGATG ATAGTTATAT TCAATACACA GAGATAAATA CAGAGCTTCG TGATTATGTT 
GTTGAAATAA CAAAAGAAGT CTTTAGGCAG CATTGTGCGA AGCATCTAGA GGTCATACCA 
ATGCGCTTAC TTAGTGATTG CCCCCAGTTT AGCAGGAAAA CTGTAAAGCT TTTGACCAAT 
GGAGGAGATA TGCTTGAACT ATGCTATGAG CTACGACTGC CTTTTGTGCA TTGGATAAGC 
GTAAATCAGA AATCCTCATT CAAGCGATAT GAAATATCTC ATGTCTACAG GAGAGCAATT 
GGCCATTCTC CACCAAATCC GTGTCTTCAG GCGGACTTTG ACATTGTTGG AGGCACACTA 
TCCCTGACAG AGGCAGAAGT TCTCAAGGTG ATAGTAGACA TCACAACCCA CATCTTTCAT 
CGCGGATCTT GTGACATTCA TTTGAATCAT GGAGATTTGC TGGATGCGAT TTGGTCCTGG 
GCAGGAATTA AGGCAGAGCA TAGACGAAAG GTTGCAGAGC TTCTTTCCAT GATGGGATCC 
TTGCGTCCTC AGTCATCTGA GCGGAAGCTA AAATGGGTTT TCATAAGGCG TCAACTTCTT 
CAGGAGTTGA AGTTACCTGA AGCTGTTGTC AATAGACTGC AGACTGTTGC TTCAAGGTTT 
TGTGGAGATG CAGATCAAGC ACTTCCTCGT TTAAGAGGGG CTCTGCGTGC TGATAGACCT 
ACCCGCAAAG CACTCGATGA GTTGTCAAAC CTCTTAACCT ACCTGAGAGT CTGGAGGATA 
GAAGAGCATG TTCATATTGA TGTTCTGATG CCACCAACTG AAAGTTATCA CCGGAATTTG 
TTTTTTCAGG TTTTCTTAAC CAAAGAAAAT AGCTCTGGGA CATCTAATGA TGGCGTTTTA 
CTTGCTGTTG GTGGTCGTTA TGATTGGTTG GTGCAGGAAG TGTGTGATCG TGAACATAAA 
ATGAACCTCC CTGGTGCTGT TGGAGTTAGT CTTGCACTGG AGACAATATT TCAGCATCTT 
CCTATGGATC TAAGGCCTAT TAGAAATGAA GTCAGCACCA GTGTACTTGT TTGTTCAAGA 
GGAGGTGGTG GTTTACTGGT CCAGCGCATG GAACTAGTTG CGGAACTATG GGAAAAAAGT 
ATAAAGGCTG AGTTTGTTCC AACACCTGAT CCAAGTCTTA CTGAGCAGTA CGAATATGCA 
AATGAACATG AAATCAAATG TCTAGTGATC ATCACAGAGT CTGGAGTAGC TCAAAATCAA 
ATAGAGTTTG TAAAGGTTCG TCACCTTGAA CTGAAGAAGG AGAAAGTGGT AGGAAGAGAA 
GAACTTGTCA AATTTCTGCT GGATGCAATG GCTGTTCAAT TTAGAAACCC CTCTGTTTGG 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCN2 ( NM_115803.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_191500.2 Arabidopsis thaliana protein kinase family protein (GCN2), mRNA.

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CloneID	OAb13910
Clone ID Related Accession (Same CDS sequence)	NM_001339969.1 , NM_115803.3
Accession Version	NM_115803.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	protein kinase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_115803.2.

ATGGGTCGCA GCAGTTCGAA GAAAAAGAAG AAACGAGGAG GTAGCGGAAG AAGGGGTCAG 
CTGAAAGACC ATGGATCTAA TGCTGATGAA GATAATGAAC TTCTATCTGA GGAGATCACT 
GCTCTCTCTG CAATATTTCA AGAGGACTGC AAAGTTGTTT CTGATTCGCG TTCGCCTCCG 
CAAATAGCAA TCAAGCTCAG GCCGTACTCA AAGGACATGG GATATGAAGA CACCGACATA 
TCTGCTATGC TTATAGTTAG GTGCTTACCA GGATATCCTT ACAAGTGCCC CAAGCTTCAG 
ATTACTCCAG AACAAGGGTT GACAACAGCT GATGCTGAGA AGCTTTTATC TCTTCTCGAG 
GACCAGGCAA ATTCCAATGC TCGTGAAGGT CGGGTTATGA TATTCAACCT GGTGGAGGCT 
GCTCAAGAGT TTTTATCAGA AATCATTCCG GAAAGTCATG ATGAGGAATC TGTTCCATGC 
TTGACTGCAC ATCGAAGCAC TCAGTTCATT GAGCAACCTA TGCTTTCAAA TATAGCAAAA 
TCCTGTTCTG GTGGACCTTT TGTGTATGGT TTTATAGACC TATTTAGTGG CTTGGAAGAT 
GCAAGAAATT GGAGTCTGAC TCCAGATGAA AATAGGGGAA TCGTATCTTC AGTACAATCT 
CACCCACTAG ACACTTCAAG AATTTTGCAT CAGAAGCCAG ACAAGAATCT GAAGCGATTT 
GAAGACCATG CTAAAGAAGA AGTTGCACTG CCTGCTCCCA TTGCCAAACT GAATACTGTT 
CAAGAGGAGA ATGTTGATGA TACAAGCATC TCTTCTTTTG ACTCAAGTAA ATCTACTGAC 
GATGTGGAAT CTGGATTATT CCAAAATGAG AAGAAGGAAT CAAATCTTCA AGATGATACA 
GCTGAAGATG ACAGCACTAA CTCCGAAAGT GAGTCGCTGG GGTCATGGTC TTCTGATTCC 
TTAGCTCAAG ATCAAGTGCC TCAGATTAGC AAGAAAGATC TGTTGATGGT CCATTTACTT 
CGAGTAGCTT GCACTTCCCG AGGACCTTTG GCTGATGCAT TACCTCAGAT AACTGATGAA 
CTGCATGAGC TTGGTATATT GTCTGAAGAA GTGTTGGATT TAGCTTCCAA ATCATCTCCA 
GACTTTAATA GAACCTTTGA ACATGCATTC AATCAAAACA TGGCCTCAAC CAGTGTTCCT 
CAGTTTTGGG AGCCACCTTC TGATTCTTGC GAGCCAAATG CATCACTCCC AAGCTCGCGA 
TATCTCAATG ATTTTGAAGA GTTGAAACCC CTTGGCCAAG GTGGTTTCGG CCACGTTGTG 
TTGTGCAAAA ATAAACTGGA TGGAAGACAA TATGCAGTGA AGAAAATTCG ACTGAAGGAC 
AAAGAGATAC CTGTCAACAG TCGGATAGTT CGAGAAGTAG CAACACTTTC CCGTTTGCAG 
CATCAGCATG TTGTACGTTA CTATCAGGCC TGGTTCGAAA CAGGAGTTGT TGATCCCTTT 
GCTGGCGCAA ATTGGGGATC AAAAACTGCA GGGAGTTCAA TGTTCAGCTA CTCAGGTGCA 
GTGTCAACTG AAATTCCTGA GCAGGACAAT AATCTTGAGT CGACTTATCT ATATATTCAA 
ATGGAATATT GTCCCAGGAC TCTCCGCCAG GTTTTTGAAT CATATAACCA CTTCGACAAA 
GACTTTGCAT GGCATTTAAT TCGCCAAATT GTGGAAGGCT TAGCTCATAT CCATGGACAA 
GGAATAATTC ATCGGGATTT TACACCTAAC AATATTTTCT TTGACGCTCG GAATGATATT 
AAAATTGGGG ATTTTGGTCT TGCAAAGTTC TTGAAGCTGG AACAGTTGGA TCAAGATGGG 
GGTTTCTCTA CGGATGTGGC TGGAAGCGGA GTCGATAGTA CTGGTCAAGC TGGTACTTAC 
TTTTACACAG CACCTGAAAT TGAGCAAGAT TGGCCTAAGA TTGATGAAAA GGCCGACATG 
TATAGCTTAG GGGTTGTGTT CTTTGAACTT TGGCATCCTT TTGGAACCGC CATGGAGAGA 
CACGTTATTT TAACTAACCT GAAGCTGAAA GGGGAGCTAC CTCTCAAATG GGTAAATGAA 
TTTCCCGAAC AGGCGTCTCT ACTGCGGCGT TTGATGTCTC CAAGTCCATC TGATCGTCCC 
TCTGCCACAG AACTTCTTAA GCATGCATTT CCTCCCAGAA TGGAATCTGA GTTACTGGAC 
AATATTCTAA GAATAATGCA AACTTCTGAA GATTCAAGTG TTTATGATAG AGTAGTAAGT 
GTGATATTTG ATGAAGAAGT ATTAGAGATG AAAAGCCATC AGTCTAGTAG ATCGAGACTC 
TGTGCAGATG ATAGTTATAT TCAATACACA GAGATAAATA CAGAGCTTCG TGATTATGTT 
GTTGAAATAA CAAAAGAAGT CTTTAGGCAG CATTGTGCGA AGCATCTAGA GGTCATACCA 
ATGCGCTTAC TTAGTGATTG CCCCCAGTTT AGCAGGAAAA CTGTAAAGCT TTTGACCAAT 
GGAGGAGATA TGCTTGAACT ATGCTATGAG CTACGACTGC CTTTTGTGCA TTGGATAAGC 
GTAAATCAGA AATCCTCATT CAAGCGATAT GAAATATCTC ATGTCTACAG GAGAGCAATT 
GGCCATTCTC CACCAAATCC GTGTCTTCAG GCGGACTTTG ACATTGTTGG AGGCACACTA 
TCCCTGACAG AGGCAGAAGT TCTCAAGGTG ATAGTAGACA TCACAACCCA CATCTTTCAT 
CGCGGATCTT GTGACATTCA TTTGAATCAT GGAGATTTGC TGGATGCGAT TTGGTCCTGG 
GCAGGAATTA AGGCAGAGCA TAGACGAAAG GTTGCAGAGC TTCTTTCCAT GATGGGATCC 
TTGCGTCCTC AGTCATCTGA GCGGAAGCTA AAATGGGTTT TCATAAGGCG TCAACTTCTT 
CAGGAGTTGA AGTTACCTGA AGCTGTTGTC AATAGACTGC AGACTGTTGC TTCAAGGTTT 
TGTGGAGATG CAGATCAAGC ACTTCCTCGT TTAAGAGGGG CTCTGCGTGC TGATAGACCT 
ACCCGCAAAG CACTCGATGA GTTGTCAAAC CTCTTAACCT ACCTGAGAGT CTGGAGGATA 
GAAGAGCATG TTCATATTGA TGTTCTGATG CCACCAACTG AAAGTTATCA CCGGAATTTG 
TTTTTTCAGG TTTTCTTAAC CAAAGAAAAT AGCTCTGGGA CATCTAATGA TGGCGTTTTA 
CTTGCTGTTG GTGGTCGTTA TGATTGGTTG GTGCAGGAAG TGTGTGATCG TGAACATAAA 
ATGAACCTCC CTGGTGCTGT TGGAGTTAGT CTTGCACTGG AGACAATATT TCAGCATCTT 
CCTATGGATC TAAGGCCTAT TAGAAATGAA GTCAGCACCA GTGTACTTGT TTGTTCAAGA 
GGAGGTGGTG GTTTACTGGT CCAGCGCATG GAACTAGTTG CGGAACTATG GGAAAAAAGT 
ATAAAGGCTG AGTTTGTTCC AACACCTGAT CCAAGTCTTA CTGAGCAGTA CGAATATGCA 
AATGAACATG AAATCAAATG TCTAGTGATC ATCACAGAGT CTGGAGTAGC TCAAAATCAA 
ATAGAGTTTG TAAAGGTTCG TCACCTTGAA CTGAAGAAGG AGAAAGTGGT AGGAAGAGAA 
GAACTTGTCA AATTTCTGCT GGATGCAATG GCTGTTCAAT TTAGAAACCC CTCTGTTTGG 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_191500.2
CDS	359..4084
Translation	MGRSSSKKKKKRGGSGRRGQLKDHGSNADEDNELLSEEITALSAIFQEDCKVVSDSRSPPQIAIKLRPYSKDMGYEDTDISAMLIVRCLPGYPYKCPKLQITPEQGLTTADAEKLLSLLEDQANSNAREGRVMIFNLVEAAQEFLSEIIPESHDEESVPCLTAHRSTQFIEQPMLSNIAKSCSGGPFVYGFIDLFSGLEDARNWSLTPDENRGIVSSVQSHPLDTSRILHQKPDKNLKRFEDHAKEEVALPAPIAKLNTVQEENVDDTSISSFDSSKSTDDVESGLFQNEKKESNLQDDTAEDDSTNSESESLGSWSSDSLAQDQVPQISKKDLLMVHLLRVACTSRGPLADALPQITDELHELGILSEEVLDLASKSSPDFNRTFEHAFNQNMASTSVPQFWEPPSDSCEPNASLPSSRYLNDFEELKPLGQGGFGHVVLCKNKLDGRQYAVKKIRLKDKEIPVNSRIVREVATLSRLQHQHVVRYYQAWFETGVVDPFAGANWGSKTAGSSMFSYSGAVSTEIPEQDNNLESTYLYIQMEYCPRTLRQVFESYNHFDKDFAWHLIRQIVEGLAHIHGQGIIHRDFTPNNIFFDARNDIKIGDFGLAKFLKLEQLDQDGGFSTDVAGSGVDSTGQAGTYFYTAPEIEQDWPKIDEKADMYSLGVVFFELWHPFGTAMERHVILTNLKLKGELPLKWVNEFPEQASLLRRLMSPSPSDRPSATELLKHAFPPRMESELLDNILRIMQTSEDSSVYDRVVSVIFDEEVLEMKSHQSSRSRLCADDSYIQYTEINTELRDYVVEITKEVFRQHCAKHLEVIPMRLLSDCPQFSRKTVKLLTNGGDMLELCYELRLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPCLQADFDIVGGTLSLTEAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRPQSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDADQALPRLRGALRADRPTRKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSGTSNDGVLLAVGGRYDWLVQEVCDREHKMNLPGAVGVSLALETIFQHLPMDLRPIRNEVSTSVLVCSRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVAQNQIEFVKVRHLELKKEKVVGREELVKFLLDAMAVQFRNPSVWS

Target ORF information:

RefSeq Version	NM_115803.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana protein kinase family protein (GCN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_115803.3

ORF Insert Sequence:

ATGGGTCGCA GCAGTTCGAA GAAAAAGAAG AAACGAGGAG GTAGCGGAAG AAGGGGTCAG 
CTGAAAGACC ATGGATCTAA TGCTGATGAA GATAATGAAC TTCTATCTGA GGAGATCACT 
GCTCTCTCTG CAATATTTCA AGAGGACTGC AAAGTTGTTT CTGATTCGCG TTCGCCTCCG 
CAAATAGCAA TCAAGCTCAG GCCGTACTCA AAGGACATGG GATATGAAGA CACCGACATA 
TCTGCTATGC TTATAGTTAG GTGCTTACCA GGATATCCTT ACAAGTGCCC CAAGCTTCAG 
ATTACTCCAG AACAAGGGTT GACAACAGCT GATGCTGAGA AGCTTTTATC TCTTCTCGAG 
GACCAGGCAA ATTCCAATGC TCGTGAAGGT CGGGTTATGA TATTCAACCT GGTGGAGGCT 
GCTCAAGAGT TTTTATCAGA AATCATTCCG GAAAGTCATG ATGAGGAATC TGTTCCATGC 
TTGACTGCAC ATCGAAGCAC TCAGTTCATT GAGCAACCTA TGCTTTCAAA TATAGCAAAA 
TCCTGTTCTG GTGGACCTTT TGTGTATGGT TTTATAGACC TATTTAGTGG CTTGGAAGAT 
GCAAGAAATT GGAGTCTGAC TCCAGATGAA AATAGGGGAA TCGTATCTTC AGTACAATCT 
CACCCACTAG ACACTTCAAG AATTTTGCAT CAGAAGCCAG ACAAGAATCT GAAGCGATTT 
GAAGACCATG CTAAAGAAGA AGTTGCACTG CCTGCTCCCA TTGCCAAACT GAATACTGTT 
CAAGAGGAGA ATGTTGATGA TACAAGCATC TCTTCTTTTG ACTCAAGTAA ATCTACTGAC 
GATGTGGAAT CTGGATTATT CCAAAATGAG AAGAAGGAAT CAAATCTTCA AGATGATACA 
GCTGAAGATG ACAGCACTAA CTCCGAAAGT GAGTCGCTGG GGTCATGGTC TTCTGATTCC 
TTAGCTCAAG ATCAAGTGCC TCAGATTAGC AAGAAAGATC TGTTGATGGT CCATTTACTT 
CGAGTAGCTT GCACTTCCCG AGGACCTTTG GCTGATGCAT TACCTCAGAT AACTGATGAA 
CTGCATGAGC TTGGTATATT GTCTGAAGAA GTGTTGGATT TAGCTTCCAA ATCATCTCCA 
GACTTTAATA GAACCTTTGA ACATGCATTC AATCAAAACA TGGCCTCAAC CAGTGTTCCT 
CAGTTTTGGG AGCCACCTTC TGATTCTTGC GAGCCAAATG CATCACTCCC AAGCTCGCGA 
TATCTCAATG ATTTTGAAGA GTTGAAACCC CTTGGCCAAG GTGGTTTCGG CCACGTTGTG 
TTGTGCAAAA ATAAACTGGA TGGAAGACAA TATGCAGTGA AGAAAATTCG ACTGAAGGAC 
AAAGAGATAC CTGTCAACAG TCGGATAGTT CGAGAAGTAG CAACACTTTC CCGTTTGCAG 
CATCAGCATG TTGTACGTTA CTATCAGGCC TGGTTCGAAA CAGGAGTTGT TGATCCCTTT 
GCTGGCGCAA ATTGGGGATC AAAAACTGCA GGGAGTTCAA TGTTCAGCTA CTCAGGTGCA 
GTGTCAACTG AAATTCCTGA GCAGGACAAT AATCTTGAGT CGACTTATCT ATATATTCAA 
ATGGAATATT GTCCCAGGAC TCTCCGCCAG GTTTTTGAAT CATATAACCA CTTCGACAAA 
GACTTTGCAT GGCATTTAAT TCGCCAAATT GTGGAAGGCT TAGCTCATAT CCATGGACAA 
GGAATAATTC ATCGGGATTT TACACCTAAC AATATTTTCT TTGACGCTCG GAATGATATT 
AAAATTGGGG ATTTTGGTCT TGCAAAGTTC TTGAAGCTGG AACAGTTGGA TCAAGATGGG 
GGTTTCTCTA CGGATGTGGC TGGAAGCGGA GTCGATAGTA CTGGTCAAGC TGGTACTTAC 
TTTTACACAG CACCTGAAAT TGAGCAAGAT TGGCCTAAGA TTGATGAAAA GGCCGACATG 
TATAGCTTAG GGGTTGTGTT CTTTGAACTT TGGCATCCTT TTGGAACCGC CATGGAGAGA 
CACGTTATTT TAACTAACCT GAAGCTGAAA GGGGAGCTAC CTCTCAAATG GGTAAATGAA 
TTTCCCGAAC AGGCGTCTCT ACTGCGGCGT TTGATGTCTC CAAGTCCATC TGATCGTCCC 
TCTGCCACAG AACTTCTTAA GCATGCATTT CCTCCCAGAA TGGAATCTGA GTTACTGGAC 
AATATTCTAA GAATAATGCA AACTTCTGAA GATTCAAGTG TTTATGATAG AGTAGTAAGT 
GTGATATTTG ATGAAGAAGT ATTAGAGATG AAAAGCCATC AGTCTAGTAG ATCGAGACTC 
TGTGCAGATG ATAGTTATAT TCAATACACA GAGATAAATA CAGAGCTTCG TGATTATGTT 
GTTGAAATAA CAAAAGAAGT CTTTAGGCAG CATTGTGCGA AGCATCTAGA GGTCATACCA 
ATGCGCTTAC TTAGTGATTG CCCCCAGTTT AGCAGGAAAA CTGTAAAGCT TTTGACCAAT 
GGAGGAGATA TGCTTGAACT ATGCTATGAG CTACGACTGC CTTTTGTGCA TTGGATAAGC 
GTAAATCAGA AATCCTCATT CAAGCGATAT GAAATATCTC ATGTCTACAG GAGAGCAATT 
GGCCATTCTC CACCAAATCC GTGTCTTCAG GCGGACTTTG ACATTGTTGG AGGCACACTA 
TCCCTGACAG AGGCAGAAGT TCTCAAGGTG ATAGTAGACA TCACAACCCA CATCTTTCAT 
CGCGGATCTT GTGACATTCA TTTGAATCAT GGAGATTTGC TGGATGCGAT TTGGTCCTGG 
GCAGGAATTA AGGCAGAGCA TAGACGAAAG GTTGCAGAGC TTCTTTCCAT GATGGGATCC 
TTGCGTCCTC AGTCATCTGA GCGGAAGCTA AAATGGGTTT TCATAAGGCG TCAACTTCTT 
CAGGAGTTGA AGTTACCTGA AGCTGTTGTC AATAGACTGC AGACTGTTGC TTCAAGGTTT 
TGTGGAGATG CAGATCAAGC ACTTCCTCGT TTAAGAGGGG CTCTGCGTGC TGATAGACCT 
ACCCGCAAAG CACTCGATGA GTTGTCAAAC CTCTTAACCT ACCTGAGAGT CTGGAGGATA 
GAAGAGCATG TTCATATTGA TGTTCTGATG CCACCAACTG AAAGTTATCA CCGGAATTTG 
TTTTTTCAGG TTTTCTTAAC CAAAGAAAAT AGCTCTGGGA CATCTAATGA TGGCGTTTTA 
CTTGCTGTTG GTGGTCGTTA TGATTGGTTG GTGCAGGAAG TGTGTGATCG TGAACATAAA 
ATGAACCTCC CTGGTGCTGT TGGAGTTAGT CTTGCACTGG AGACAATATT TCAGCATCTT 
CCTATGGATC TAAGGCCTAT TAGAAATGAA GTCAGCACCA GTGTACTTGT TTGTTCAAGA 
GGAGGTGGTG GTTTACTGGT CCAGCGCATG GAACTAGTTG CGGAACTATG GGAAAAAAGT 
ATAAAGGCTG AGTTTGTTCC AACACCTGAT CCAAGTCTTA CTGAGCAGTA CGAATATGCA 
AATGAACATG AAATCAAATG TCTAGTGATC ATCACAGAGT CTGGAGTAGC TCAAAATCAA 
ATAGAGTTTG TAAAGGTTCG TCACCTTGAA CTGAAGAAGG AGAAAGTGGT AGGAAGAGAA 
GAACTTGTCA AATTTCTGCT GGATGCAATG GCTGTTCAAT TTAGAAACCC CTCTGTTTGG 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCN2 ( NM_001203206.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190135.1 Arabidopsis thaliana protein kinase family protein (GCN2), mRNA.

Price & Availability↑

CloneID	OAb29946
Clone ID Related Accession (Same CDS sequence)	NM_001203206.1
Accession Version	NM_001203206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	protein kinase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGGGTCGCA GCAGTTCGAA GAAAAAGAAG AAACGAGGAG GTAGCGGAAG AAGGGGTCAG 
CTGAAAGACC ATGGATCTAA TGCTGATGAA GATAATGAAC TTCTATCTGA GGAGATCACT 
GCTCTAAATT GTGTAAATGT TGGGATTATG GGATTTGATC GTTCAGATGT TACAGGGAAA 
AACAATAGCA ATTATTTCTC TGCAATATTT CAAGAGGACT GCAAAGTTGT TTCTGATTCG 
CGTTCGCCTC CGCAAATAGC AATCAAGCTC AGGCCGTACT CAAAGGACAT GGGATATGAA 
GACACCGACA TATCTGCTAT GCTTATAGTT AGGTGCTTAC CAGGATATCC TTACAAGTGC 
CCCAAGCTTC AGATTACTCC AGAACAAGGG TTGACAACAG CTGATGCTGA GAAGCTTTTA 
TCTCTTCTCG AGGACCAGGC AAATTCCAAT GCTCGTGAAG GTCGGGTTAT GATATTCAAC 
CTGGTGGAGG CTGCTCAAGA GTTTTTATCA GAAATCATTC CGGAAAGTCA TGATGAGGAA 
TCTGTTCCAT GCTTGACTGC ACATCGAAGC ACTCAGTTCA TTGAGCAACC TATGCTTTCA 
AATATAGCAA AATCCTGTTC TGGTGGACCT TTTGTGTATG GTTTTATAGA CCTATTTAGT 
GGCTTGGAAG ATGCAAGAAA TTGGAGTCTG ACTCCAGATG AAAATAGGGG AATCGTATCT 
TCAGTACAAT CTCACCCACT AGACACTTCA AGAATTTTGC ATCAGAAGCC AGACAAGAAT 
CTGAAGCGAT TTGAAGACCA TGCTAAAGAA GAAGTTGCAC TGCCTGCTCC CATTGCCAAA 
CTGAATACTG TTCAAGAGGA GAATGTTGAT GATACAAGCA TCTCTTCTTT TGACTCAAGT 
AAATCTACTG ACGATGTGGA ATCTGGATTA TTCCAAAATG AGAAGAAGGA ATCAAATCTT 
CAAGATGATA CAGCTGAAGA TGACAGCACT AACTCCGAAA GTGAGTCGCT GGGGTCATGG 
TCTTCTGATT CCTTAGCTCA AGATCAAGTG CCTCAGATTA GCAAGAAAGA TCTGTTGATG 
GTCCATTTAC TTCGAGTAGC TTGCACTTCC CGAGGACCTT TGGCTGATGC ATTACCTCAG 
ATAACTGATG AACTGCATGA GCTTGGTATA TTGTCTGAAG AAGTGTTGGA TTTAGCTTCC 
AAATCATCTC CAGACTTTAA TAGAACCTTT GAACATGCAT TCAATCAAAA CATGGCCTCA 
ACCAGTGTTC CTCAGTTTTG GGAGCCACCT TCTGATTCTT GCGAGCCAAA TGCATCACTC 
CCAAGCTCGC GATATCTCAA TGATTTTGAA GAGTTGAAAC CCCTTGGCCA AGGTGGTTTC 
GGCCACGTTG TGTTGTGCAA AAATAAACTG GATGGAAGAC AATATGCAGT GAAGAAAATT 
CGACTGAAGG ACAAAGAGAT ACCTGTCAAC AGTCGGATAG TTCGAGAAGT AGCAACACTT 
TCCCGTTTGC AGCATCAGCA TGTTGTACGT TACTATCAGG CCTGGTTCGA AACAGGAGTT 
GTTGATCCCT TTGCTGGCGC AAATTGGGGA TCAAAAACTG CAGGGAGTTC AATGTTCAGC 
TACTCAGGTG CAGTGTCAAC TGAAATTCCT GAGCAGGACA ATAATCTTGA GTCGACTTAT 
CTATATATTC AAATGGAATA TTGTCCCAGG ACTCTCCGCC AGGTTTTTGA ATCATATAAC 
CACTTCGACA AAGACTTTGC ATGGCATTTA ATTCGCCAAA TTGTGGAAGG CTTAGCTCAT 
ATCCATGGAC AAGGAATAAT TCATCGGGAT TTTACACCTA ACAATATTTT CTTTGACGCT 
CGGAATGATA TTAAAATTGG GGATTTTGGT CTTGCAAAGT TCTTGAAGCT GGAACAGTTG 
GATCAAGATG GGGGTTTCTC TACGGATGTG GCTGGAAGCG GAGTCGATAG TACTGGTCAA 
GCTGGTACTT ACTTTTACAC AGCACCTGAA ATTGAGCAAG ATTGGCCTAA GATTGATGAA 
AAGGCCGACA TGTATAGCTT AGGGGTTGTG TTCTTTGAAC TTTGGCATCC TTTTGGAACC 
GCCATGGAGA GACACGTTAT TTTAACTAAC CTGAAGCTGA AAGGGGAGCT ACCTCTCAAA 
TGGGTAAATG AATTTCCCGA ACAGGCGTCT CTACTGCGGC GTTTGATGTC TCCAAGTCCA 
TCTGATCGTC CCTCTGCCAC AGAACTTCTT AAGCATGCAT TTCCTCCCAG AATGGAATCT 
GAGTTACTGG ACAATATTCT AAGAATAATG CAAACTTCTG AAGATTCAAG TGTTTATGAT 
AGAGTAGTAA GTGTGATATT TGATGAAGAA GTATTAGAGA TGAAAAGCCA TCAGTCTAGT 
AGATCGAGAC TCTGTGCAGA TGATAGTTAT ATTCAATACA CAGAGATAAA TACAGAGCTT 
CGTGATTATG TTGTTGAAAT AACAAAAGAA GTCTTTAGGC AGCATTGTGC GAAGCATCTA 
GAGGTCATAC CAATGCGCTT ACTTAGTGAT TGCCCCCAGT TTAGCAGGAA AACTGTAAAG 
CTTTTGACCA ATGGAGGAGA TATGCTTGAA CTATGCTATG AGCTACGACT GCCTTTTGTG 
CATTGGATAA GCGTAAATCA GAAATCCTCA TTCAAGCGAT ATGAAATATC TCATGTCTAC 
AGGAGAGCAA TTGGCCATTC TCCACCAAAT CCGTGTCTTC AGGCGGACTT TGACATTGTT 
GGAGGCACAC TATCCCTGAC AGAGGCAGAA GTTCTCAAGG TGATAGTAGA CATCACAACC 
CACATCTTTC ATCGCGGATC TTGTGACATT CATTTGAATC ATGGAGATTT GCTGGATGCG 
ATTTGGTCCT GGGCAGGAAT TAAGGCAGAG CATAGACGAA AGGTTGCAGA GCTTCTTTCC 
ATGATGGGAT CCTTGCGTCC TCAGTCATCT GAGCGGAAGC TAAAATGGGT TTTCATAAGG 
CGTCAACTTC TTCAGGAGTT GAAGTTACCT GAAGCTGTTG TCAATAGACT GCAGACTGTT 
GCTTCAAGGT TTTGTGGAGA TGCAGATCAA GCACTTCCTC GTTTAAGAGG GGCTCTGCGT 
GCTGATAGAC CTACCCGCAA AGCACTCGAT GAGTTGTCAA ACCTCTTAAC CTACCTGAGA 
GTCTGGAGGA TAGAAGAGCA TGTTCATATT GATGTTCTGA TGCCACCAAC TGAAAGTTAT 
CACCGGAATT TGTTTTTTCA GGTTTTCTTA ACCAAAGAAA ATAGCTCTGG GACATCTAAT 
GATGGCGTTT TACTTGCTGT TGGTGGTCGT TATGATTGGT TGGTGCAGGA AGTGTGTGAT 
CGTGAACATA AAATGAACCT CCCTGGTGCT GTTGGAGTTA GTCTTGCACT GGAGACAATA 
TTTCAGCATC TTCCTATGGA TCTAAGGCCT ATTAGAAATG AAGTCAGCAC CAGTGTACTT 
GTTTGTTCAA GAGGAGGTGG TGGTTTACTG GTCCAGCGCA TGGAACTAGT TGCGGAACTA 
TGGGAAAAAA GTATAAAGGC TGAGTTTGTT CCAACACCTG ATCCAAGTCT TACTGAGCAG 
TACGAATATG CAAATGAACA TGAAATCAAA TGTCTAGTGA TCATCACAGA GTCTGGAGTA 
GCTCAAAATC AAATAGAGTT TGTAAAGGTT CGTCACCTTG AACTGAAGAA GGAGAAAGTG 
GTAGGAAGAG AAGAACTTGT CAAATTTCTG CTGGATGCAA TGGCTGTTCA ATTTAGAAAC 
CCCTCTGTTT GGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190135.1
CDS	388..4185
Translation	MGRSSSKKKKKRGGSGRRGQLKDHGSNADEDNELLSEEITALNCVNVGIMGFDRSDVTGKNNSNYFSAIFQEDCKVVSDSRSPPQIAIKLRPYSKDMGYEDTDISAMLIVRCLPGYPYKCPKLQITPEQGLTTADAEKLLSLLEDQANSNAREGRVMIFNLVEAAQEFLSEIIPESHDEESVPCLTAHRSTQFIEQPMLSNIAKSCSGGPFVYGFIDLFSGLEDARNWSLTPDENRGIVSSVQSHPLDTSRILHQKPDKNLKRFEDHAKEEVALPAPIAKLNTVQEENVDDTSISSFDSSKSTDDVESGLFQNEKKESNLQDDTAEDDSTNSESESLGSWSSDSLAQDQVPQISKKDLLMVHLLRVACTSRGPLADALPQITDELHELGILSEEVLDLASKSSPDFNRTFEHAFNQNMASTSVPQFWEPPSDSCEPNASLPSSRYLNDFEELKPLGQGGFGHVVLCKNKLDGRQYAVKKIRLKDKEIPVNSRIVREVATLSRLQHQHVVRYYQAWFETGVVDPFAGANWGSKTAGSSMFSYSGAVSTEIPEQDNNLESTYLYIQMEYCPRTLRQVFESYNHFDKDFAWHLIRQIVEGLAHIHGQGIIHRDFTPNNIFFDARNDIKIGDFGLAKFLKLEQLDQDGGFSTDVAGSGVDSTGQAGTYFYTAPEIEQDWPKIDEKADMYSLGVVFFELWHPFGTAMERHVILTNLKLKGELPLKWVNEFPEQASLLRRLMSPSPSDRPSATELLKHAFPPRMESELLDNILRIMQTSEDSSVYDRVVSVIFDEEVLEMKSHQSSRSRLCADDSYIQYTEINTELRDYVVEITKEVFRQHCAKHLEVIPMRLLSDCPQFSRKTVKLLTNGGDMLELCYELRLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPCLQADFDIVGGTLSLTEAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRPQSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDADQALPRLRGALRADRPTRKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSGTSNDGVLLAVGGRYDWLVQEVCDREHKMNLPGAVGVSLALETIFQHLPMDLRPIRNEVSTSVLVCSRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVAQNQIEFVKVRHLELKKEKVVGREELVKFLLDAMAVQFRNPSVWS

Target ORF information:

RefSeq Version	NM_001203206.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana protein kinase family protein (GCN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203206.1

ORF Insert Sequence:

ATGGGTCGCA GCAGTTCGAA GAAAAAGAAG AAACGAGGAG GTAGCGGAAG AAGGGGTCAG 
CTGAAAGACC ATGGATCTAA TGCTGATGAA GATAATGAAC TTCTATCTGA GGAGATCACT 
GCTCTAAATT GTGTAAATGT TGGGATTATG GGATTTGATC GTTCAGATGT TACAGGGAAA 
AACAATAGCA ATTATTTCTC TGCAATATTT CAAGAGGACT GCAAAGTTGT TTCTGATTCG 
CGTTCGCCTC CGCAAATAGC AATCAAGCTC AGGCCGTACT CAAAGGACAT GGGATATGAA 
GACACCGACA TATCTGCTAT GCTTATAGTT AGGTGCTTAC CAGGATATCC TTACAAGTGC 
CCCAAGCTTC AGATTACTCC AGAACAAGGG TTGACAACAG CTGATGCTGA GAAGCTTTTA 
TCTCTTCTCG AGGACCAGGC AAATTCCAAT GCTCGTGAAG GTCGGGTTAT GATATTCAAC 
CTGGTGGAGG CTGCTCAAGA GTTTTTATCA GAAATCATTC CGGAAAGTCA TGATGAGGAA 
TCTGTTCCAT GCTTGACTGC ACATCGAAGC ACTCAGTTCA TTGAGCAACC TATGCTTTCA 
AATATAGCAA AATCCTGTTC TGGTGGACCT TTTGTGTATG GTTTTATAGA CCTATTTAGT 
GGCTTGGAAG ATGCAAGAAA TTGGAGTCTG ACTCCAGATG AAAATAGGGG AATCGTATCT 
TCAGTACAAT CTCACCCACT AGACACTTCA AGAATTTTGC ATCAGAAGCC AGACAAGAAT 
CTGAAGCGAT TTGAAGACCA TGCTAAAGAA GAAGTTGCAC TGCCTGCTCC CATTGCCAAA 
CTGAATACTG TTCAAGAGGA GAATGTTGAT GATACAAGCA TCTCTTCTTT TGACTCAAGT 
AAATCTACTG ACGATGTGGA ATCTGGATTA TTCCAAAATG AGAAGAAGGA ATCAAATCTT 
CAAGATGATA CAGCTGAAGA TGACAGCACT AACTCCGAAA GTGAGTCGCT GGGGTCATGG 
TCTTCTGATT CCTTAGCTCA AGATCAAGTG CCTCAGATTA GCAAGAAAGA TCTGTTGATG 
GTCCATTTAC TTCGAGTAGC TTGCACTTCC CGAGGACCTT TGGCTGATGC ATTACCTCAG 
ATAACTGATG AACTGCATGA GCTTGGTATA TTGTCTGAAG AAGTGTTGGA TTTAGCTTCC 
AAATCATCTC CAGACTTTAA TAGAACCTTT GAACATGCAT TCAATCAAAA CATGGCCTCA 
ACCAGTGTTC CTCAGTTTTG GGAGCCACCT TCTGATTCTT GCGAGCCAAA TGCATCACTC 
CCAAGCTCGC GATATCTCAA TGATTTTGAA GAGTTGAAAC CCCTTGGCCA AGGTGGTTTC 
GGCCACGTTG TGTTGTGCAA AAATAAACTG GATGGAAGAC AATATGCAGT GAAGAAAATT 
CGACTGAAGG ACAAAGAGAT ACCTGTCAAC AGTCGGATAG TTCGAGAAGT AGCAACACTT 
TCCCGTTTGC AGCATCAGCA TGTTGTACGT TACTATCAGG CCTGGTTCGA AACAGGAGTT 
GTTGATCCCT TTGCTGGCGC AAATTGGGGA TCAAAAACTG CAGGGAGTTC AATGTTCAGC 
TACTCAGGTG CAGTGTCAAC TGAAATTCCT GAGCAGGACA ATAATCTTGA GTCGACTTAT 
CTATATATTC AAATGGAATA TTGTCCCAGG ACTCTCCGCC AGGTTTTTGA ATCATATAAC 
CACTTCGACA AAGACTTTGC ATGGCATTTA ATTCGCCAAA TTGTGGAAGG CTTAGCTCAT 
ATCCATGGAC AAGGAATAAT TCATCGGGAT TTTACACCTA ACAATATTTT CTTTGACGCT 
CGGAATGATA TTAAAATTGG GGATTTTGGT CTTGCAAAGT TCTTGAAGCT GGAACAGTTG 
GATCAAGATG GGGGTTTCTC TACGGATGTG GCTGGAAGCG GAGTCGATAG TACTGGTCAA 
GCTGGTACTT ACTTTTACAC AGCACCTGAA ATTGAGCAAG ATTGGCCTAA GATTGATGAA 
AAGGCCGACA TGTATAGCTT AGGGGTTGTG TTCTTTGAAC TTTGGCATCC TTTTGGAACC 
GCCATGGAGA GACACGTTAT TTTAACTAAC CTGAAGCTGA AAGGGGAGCT ACCTCTCAAA 
TGGGTAAATG AATTTCCCGA ACAGGCGTCT CTACTGCGGC GTTTGATGTC TCCAAGTCCA 
TCTGATCGTC CCTCTGCCAC AGAACTTCTT AAGCATGCAT TTCCTCCCAG AATGGAATCT 
GAGTTACTGG ACAATATTCT AAGAATAATG CAAACTTCTG AAGATTCAAG TGTTTATGAT 
AGAGTAGTAA GTGTGATATT TGATGAAGAA GTATTAGAGA TGAAAAGCCA TCAGTCTAGT 
AGATCGAGAC TCTGTGCAGA TGATAGTTAT ATTCAATACA CAGAGATAAA TACAGAGCTT 
CGTGATTATG TTGTTGAAAT AACAAAAGAA GTCTTTAGGC AGCATTGTGC GAAGCATCTA 
GAGGTCATAC CAATGCGCTT ACTTAGTGAT TGCCCCCAGT TTAGCAGGAA AACTGTAAAG 
CTTTTGACCA ATGGAGGAGA TATGCTTGAA CTATGCTATG AGCTACGACT GCCTTTTGTG 
CATTGGATAA GCGTAAATCA GAAATCCTCA TTCAAGCGAT ATGAAATATC TCATGTCTAC 
AGGAGAGCAA TTGGCCATTC TCCACCAAAT CCGTGTCTTC AGGCGGACTT TGACATTGTT 
GGAGGCACAC TATCCCTGAC AGAGGCAGAA GTTCTCAAGG TGATAGTAGA CATCACAACC 
CACATCTTTC ATCGCGGATC TTGTGACATT CATTTGAATC ATGGAGATTT GCTGGATGCG 
ATTTGGTCCT GGGCAGGAAT TAAGGCAGAG CATAGACGAA AGGTTGCAGA GCTTCTTTCC 
ATGATGGGAT CCTTGCGTCC TCAGTCATCT GAGCGGAAGC TAAAATGGGT TTTCATAAGG 
CGTCAACTTC TTCAGGAGTT GAAGTTACCT GAAGCTGTTG TCAATAGACT GCAGACTGTT 
GCTTCAAGGT TTTGTGGAGA TGCAGATCAA GCACTTCCTC GTTTAAGAGG GGCTCTGCGT 
GCTGATAGAC CTACCCGCAA AGCACTCGAT GAGTTGTCAA ACCTCTTAAC CTACCTGAGA 
GTCTGGAGGA TAGAAGAGCA TGTTCATATT GATGTTCTGA TGCCACCAAC TGAAAGTTAT 
CACCGGAATT TGTTTTTTCA GGTTTTCTTA ACCAAAGAAA ATAGCTCTGG GACATCTAAT 
GATGGCGTTT TACTTGCTGT TGGTGGTCGT TATGATTGGT TGGTGCAGGA AGTGTGTGAT 
CGTGAACATA AAATGAACCT CCCTGGTGCT GTTGGAGTTA GTCTTGCACT GGAGACAATA 
TTTCAGCATC TTCCTATGGA TCTAAGGCCT ATTAGAAATG AAGTCAGCAC CAGTGTACTT 
GTTTGTTCAA GAGGAGGTGG TGGTTTACTG GTCCAGCGCA TGGAACTAGT TGCGGAACTA 
TGGGAAAAAA GTATAAAGGC TGAGTTTGTT CCAACACCTG ATCCAAGTCT TACTGAGCAG 
TACGAATATG CAAATGAACA TGAAATCAAA TGTCTAGTGA TCATCACAGA GTCTGGAGTA 
GCTCAAAATC AAATAGAGTT TGTAAAGGTT CGTCACCTTG AACTGAAGAA GGAGAAAGTG 
GTAGGAAGAG AAGAACTTGT CAAATTTCTG CTGGATGCAA TGGCTGTTCA ATTTAGAAAC 
CCCTCTGTTT GGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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GCN2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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