NEK6 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEK6
Entrez Gene ID	823542
Full Name	serine/threonine-protein kinase NEK5
Synonyms	''NIMA (never in mitosis,ATNEK6,IBO1,NIMA (never in mitosis,NIMA-RELATED KINASE6,gene A)-related 6,gene A)-related 6''
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001339123.1	NP_001327688.1	serine/threonine-protein kinase NEK5
NM_114288.4	NP_190006.2	serine/threonine-protein kinase NEK5

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	NEK6	NP_190006.2
Oryza sativa (rice)	Os05g0440800	NP_001055669.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data demonstrated that NEK6 is required for the directional growth of roots and hypocotyls, petiole elongation, cell file formation, and trichome morphogenesis.
Title: NIMA-related kinases regulate directional cell growth and organ development through microtubule function in Arabidopsis thaliana.

NEK6 regulates rosette growth and yield-related traits in Arabidopsis.
Title: NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis.

NEK6 homodimerizes and forms heterodimers with NEK4 and NEK5 to regulate cortical microtubule organization possibly through the phosphorylation of beta-tubulins.
Title: NIMA-related kinases 6, 4, and 5 interact with each other to regulate microtubule organization during epidermal cell expansion in Arabidopsis thaliana.

interacts with ARIA and is involved in abscisic acid response during seed germination
Title: AtNEK6 interacts with ARIA and is involved in ABA response during seed germination.

ibo1-1 mutation, in which a conserved Glu residue in the activation loop is substituted by Arg, completely abolishes its kinase activity
Title: A NIMA-related protein kinase suppresses ectopic outgrowth of epidermal cells through its kinase activity and the association with microtubules.

The following NEK6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEK6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb12791	NM_001339123.1 Latest version!	Arabidopsis thaliana serine/threonine-protein kinase NEK5 (NEK6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb12791	NM_114288.4 Latest version!	Arabidopsis thaliana serine/threonine-protein kinase NEK5 (NEK6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEK6 ( NM_001339123.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001327688.1 Arabidopsis thaliana serine/threonine-protein kinase NEK5 (NEK6), mRNA.

Price & Availability↑

CloneID	OAb12791
Clone ID Related Accession (Same CDS sequence)	NM_001339123.1 , NM_114288.4
Accession Version	NM_001339123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	serine/threonine-protein kinase NEK5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGGAGTCAC GAATGGATCA GTACGAGCTT ATGGAACAGA TCGGGAGAGG AGCGTTTGGT 
GCTGCTATTT TAGTTCATCA TAAAGCTGAG AGAAAGAAGT ATGTTTTGAA GAAGATCAGA 
TTAGCTAGAC AAACGGAACG TTGCCGGAGA TCTGCTCATC AAGAGATGTC TTTGATTGCA 
AGAGTTCAAC ATCCTTATAT TGTGGAGTTT AAAGAAGCTT GGGTTGAGAA AGGTTGCTAT 
GTTTGTATTG TCACTGGATA CTGTGAAGGA GGAGACATGG CTGAGTTGAT GAAAAAGTCA 
AATGGTGTTT ATTTTCCTGA GGAGAAACTT TGCAAGTGGT TTACTCAATT ATTATTAGCT 
GTTGAGTATC TGCATTCTAA CTATGTTCTA CATCGGGATC TAAAGTGTTC CAACATTTTC 
CTTACAAAAG ATCAAGATGT TCGCCTTGGG GACTTTGGTC TTGCCAAAAC TTTGAAGGCG 
GATGACCTAA CTTCCTCGGT TGTTGGAACT CCAAACTACA TGTGCCCGGA ACTGCTTGCT 
GATATCCCTT ATGGTTTTAA GTCAGATATC TGGTCCTTAG GCTGTTGTAT ATATGAGATG 
GCTGCGTATC GACCTGCTTT CAAAGCTTTT GATATGGCAG GGCTTATAAG CAAGGTTAAT 
CGCTCCTCAA TTGGTCCGTT GCCTCCGTGC TATTCACCAT CTTTAAAAGC ACTCATCAAG 
GGAATGTTAA GGAAGAACCC CGAGTATCGG CCAAACGCCT CCGAGATTTT GAAGCATCCT 
TATCTGCAGC CATATGTTGA GCAGTACCGT CCAACGCTTT CTGCGGCGTC TATAACTCCA 
GAAAAGCCTC TCAATTCTCG CGAGGGTCGG AGGAGTATGG CTGAAAGTCA GAATAGCAAT 
AGTTCAAGTG AAAAAGATAA CTTCTACGTG AGTGACAAAA ATATCCGATA TGTGGTTCCA 
AGTAATGGTA ATAAAGTCAC TGAGACCGAT TCAGGTTTTG TCGATGATGA AGACATCTTA 
GACCATGTGC AACAATCAGC TGAAAATGGT AATCTCCAGA GTGTTTCAGC AACAAAACCA 
GATGGCCATG GGATTTTAAA GCCCGTACAC AGTGACCAGC GACCAGACGT TATTCAACCA 
AGGCACCCAA AAACTATCAG AAACATCATG ATGGTTTTGA AAGAAGAGAA AGCTCGAGAA 
AATGGTTCAC CCATGAGATC TAATCGAAGT AGACCTTCTA GTGTTCCGAC ACAGAAGAAT 
AATGTTGAAA CTCCATCAAA GATTCCCAAA CTTGGTGACA TTGCTCATAG CTCCAAGACT 
AACGCAAGTA CGCCTATTCC ACCATCAAAG CTGGCCTCTG ATTCCGCAAG GACTCCAGGA 
TCATTTCCAC CAAAGCATCA TATGCCAGTG ATTGACTCTT CTCCAAAACT TAAGCCTAGA 
AACGACAGAA TTTCACCTTC TCCTGCTGCT AAACATGAGG CTGAAGAGGC GATGTCAGTT 
AAGCGTAGGC AAAGAACACC TCCTACTTTG CCAAGAAGAA CGTCTTTGAT AGCGCACCAG 
TCGAGACAAC TAGGAGCAGA TATTTCAAAT ATGGCAGCAA AGGAAACCGC GAAGCTACAT 
CCATCTGTGC CATCAGAGTC TGAAACTAAT TCTCATCAAT CTCGTGTTCA TGCTTCTCCT 
GTGAGTACGA CGCCAGAGCC AAAGAGAACC TCTGTTGGAT CTGCCAAAGG AATGCAGAGT 
GAAAGCAGCA ACTCTATCTC GTCGTCGTTG TCCATGCAGG CATTTGAGCT CTGTGATGAT 
GCTTCCACCC CATATATTGA CATGACAGAA CATACAACTC CTGATGACCA CAGAAGATCC 
TGTCACAGTG AATACTCATA TTCATTTCCA GATATCTCCT CAGAGATGAT GATCCGTAGA 
GATGAACATA GCACCAGTAT GCGGCTGACT GAAATTCCCG ACTCTGTTTC CGGTGTACAA 
AACACCATTG CCCATCATCA GCCAGAAAGA GAGCAAGGAA GCTGTCCCAC TGTGCTCAAA 
GATGATAGTC CTGCAACATT ACAGAGCTAC GAGCCTAACA CATCACAACA TCAACACGGT 
GATGACAAAT TCACAGTCAA AGAATTCGTC TCCTCTGTTC CCGGACCCGC ACCATTGCCT 
TTGCATGTTG AACCATCACA CCAAGTGAAT TCCCACTCGG ATAACAAAAC AAGCGTAATG 
TCTCAGAACT CAGCTCTTGA GAAGAACAAC AGTCATTCCC ATCCTCATCC CGTGGTTGAT 
GATGTCATAC ATGTTATTCG CCACAGCAGT TTCCGAGTAG GGAGCGACCA GCCGGTCATG 
GAAAGTGTTG AAGTCGGTGT CCAAAACGTC GATATGGGGA AACTCATAAA CGTTGTGAGA 
GATGAAATGG AAGTGAGAAA AGGAGCCACT CCCTCAGAAT CACCCACCAC AAGATCGATC 
ATCTCAGAGC CTGACTCAAG AACCGAGCCT CGTCCTAAAG AACCAGACCC CATCACCAAT 
TACTCAGAAA CAAAGAGCTT TAACTCCTGC TCGGATTCTT CACCAGCTGA GACCAGAACT 
AACTCATTCG TGCCTGAAGA GGAAACAACT CCGACTCCAC CTGTTAAAGA GACGTTGGAC 
ATCAAGTCGT TCAGACAGAG AGCAGAAGCG CTTGAAGGTC TATTGGAACT ATCTGCGGAT 
TTGCTGGAAC AGAGCAGGCT CGAAGAGCTA GCCATTGTGT TGCAGCCGTT TGGGAAGAAC 
AAAGTTTCGC CTCGAGAAAC CGCTATTTGG TTGGCCAAGA GTTTGAAAGG AATGATGATC 
GAAGACATCA ATAATAATAA TAGCAGTGGC AGCTCCAGGA ATTGTTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001327688.1
CDS	431..3301
Translation	MESRMDQYELMEQIGRGAFGAAILVHHKAERKKYVLKKIRLARQTERCRRSAHQEMSLIARVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGVYFPEEKLCKWFTQLLLAVEYLHSNYVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLTSSVVGTPNYMCPELLADIPYGFKSDIWSLGCCIYEMAAYRPAFKAFDMAGLISKVNRSSIGPLPPCYSPSLKALIKGMLRKNPEYRPNASEILKHPYLQPYVEQYRPTLSAASITPEKPLNSREGRRSMAESQNSNSSSEKDNFYVSDKNIRYVVPSNGNKVTETDSGFVDDEDILDHVQQSAENGNLQSVSATKPDGHGILKPVHSDQRPDVIQPRHPKTIRNIMMVLKEEKARENGSPMRSNRSRPSSVPTQKNNVETPSKIPKLGDIAHSSKTNASTPIPPSKLASDSARTPGSFPPKHHMPVIDSSPKLKPRNDRISPSPAAKHEAEEAMSVKRRQRTPPTLPRRTSLIAHQSRQLGADISNMAAKETAKLHPSVPSESETNSHQSRVHASPVSTTPEPKRTSVGSAKGMQSESSNSISSSLSMQAFELCDDASTPYIDMTEHTTPDDHRRSCHSEYSYSFPDISSEMMIRRDEHSTSMRLTEIPDSVSGVQNTIAHHQPEREQGSCPTVLKDDSPATLQSYEPNTSQHQHGDDKFTVKEFVSSVPGPAPLPLHVEPSHQVNSHSDNKTSVMSQNSALEKNNSHSHPHPVVDDVIHVIRHSSFRVGSDQPVMESVEVGVQNVDMGKLINVVRDEMEVRKGATPSESPTTRSIISEPDSRTEPRPKEPDPITNYSETKSFNSCSDSSPAETRTNSFVPEEETTPTPPVKETLDIKSFRQRAEALEGLLELSADLLEQSRLEELAIVLQPFGKNKVSPRETAIWLAKSLKGMMIEDINNNNSSGSSRNCS

Target ORF information:

RefSeq Version	NM_001339123.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana serine/threonine-protein kinase NEK5 (NEK6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001339123.1

ORF Insert Sequence:

ATGGAGTCAC GAATGGATCA GTACGAGCTT ATGGAACAGA TCGGGAGAGG AGCGTTTGGT 
GCTGCTATTT TAGTTCATCA TAAAGCTGAG AGAAAGAAGT ATGTTTTGAA GAAGATCAGA 
TTAGCTAGAC AAACGGAACG TTGCCGGAGA TCTGCTCATC AAGAGATGTC TTTGATTGCA 
AGAGTTCAAC ATCCTTATAT TGTGGAGTTT AAAGAAGCTT GGGTTGAGAA AGGTTGCTAT 
GTTTGTATTG TCACTGGATA CTGTGAAGGA GGAGACATGG CTGAGTTGAT GAAAAAGTCA 
AATGGTGTTT ATTTTCCTGA GGAGAAACTT TGCAAGTGGT TTACTCAATT ATTATTAGCT 
GTTGAGTATC TGCATTCTAA CTATGTTCTA CATCGGGATC TAAAGTGTTC CAACATTTTC 
CTTACAAAAG ATCAAGATGT TCGCCTTGGG GACTTTGGTC TTGCCAAAAC TTTGAAGGCG 
GATGACCTAA CTTCCTCGGT TGTTGGAACT CCAAACTACA TGTGCCCGGA ACTGCTTGCT 
GATATCCCTT ATGGTTTTAA GTCAGATATC TGGTCCTTAG GCTGTTGTAT ATATGAGATG 
GCTGCGTATC GACCTGCTTT CAAAGCTTTT GATATGGCAG GGCTTATAAG CAAGGTTAAT 
CGCTCCTCAA TTGGTCCGTT GCCTCCGTGC TATTCACCAT CTTTAAAAGC ACTCATCAAG 
GGAATGTTAA GGAAGAACCC CGAGTATCGG CCAAACGCCT CCGAGATTTT GAAGCATCCT 
TATCTGCAGC CATATGTTGA GCAGTACCGT CCAACGCTTT CTGCGGCGTC TATAACTCCA 
GAAAAGCCTC TCAATTCTCG CGAGGGTCGG AGGAGTATGG CTGAAAGTCA GAATAGCAAT 
AGTTCAAGTG AAAAAGATAA CTTCTACGTG AGTGACAAAA ATATCCGATA TGTGGTTCCA 
AGTAATGGTA ATAAAGTCAC TGAGACCGAT TCAGGTTTTG TCGATGATGA AGACATCTTA 
GACCATGTGC AACAATCAGC TGAAAATGGT AATCTCCAGA GTGTTTCAGC AACAAAACCA 
GATGGCCATG GGATTTTAAA GCCCGTACAC AGTGACCAGC GACCAGACGT TATTCAACCA 
AGGCACCCAA AAACTATCAG AAACATCATG ATGGTTTTGA AAGAAGAGAA AGCTCGAGAA 
AATGGTTCAC CCATGAGATC TAATCGAAGT AGACCTTCTA GTGTTCCGAC ACAGAAGAAT 
AATGTTGAAA CTCCATCAAA GATTCCCAAA CTTGGTGACA TTGCTCATAG CTCCAAGACT 
AACGCAAGTA CGCCTATTCC ACCATCAAAG CTGGCCTCTG ATTCCGCAAG GACTCCAGGA 
TCATTTCCAC CAAAGCATCA TATGCCAGTG ATTGACTCTT CTCCAAAACT TAAGCCTAGA 
AACGACAGAA TTTCACCTTC TCCTGCTGCT AAACATGAGG CTGAAGAGGC GATGTCAGTT 
AAGCGTAGGC AAAGAACACC TCCTACTTTG CCAAGAAGAA CGTCTTTGAT AGCGCACCAG 
TCGAGACAAC TAGGAGCAGA TATTTCAAAT ATGGCAGCAA AGGAAACCGC GAAGCTACAT 
CCATCTGTGC CATCAGAGTC TGAAACTAAT TCTCATCAAT CTCGTGTTCA TGCTTCTCCT 
GTGAGTACGA CGCCAGAGCC AAAGAGAACC TCTGTTGGAT CTGCCAAAGG AATGCAGAGT 
GAAAGCAGCA ACTCTATCTC GTCGTCGTTG TCCATGCAGG CATTTGAGCT CTGTGATGAT 
GCTTCCACCC CATATATTGA CATGACAGAA CATACAACTC CTGATGACCA CAGAAGATCC 
TGTCACAGTG AATACTCATA TTCATTTCCA GATATCTCCT CAGAGATGAT GATCCGTAGA 
GATGAACATA GCACCAGTAT GCGGCTGACT GAAATTCCCG ACTCTGTTTC CGGTGTACAA 
AACACCATTG CCCATCATCA GCCAGAAAGA GAGCAAGGAA GCTGTCCCAC TGTGCTCAAA 
GATGATAGTC CTGCAACATT ACAGAGCTAC GAGCCTAACA CATCACAACA TCAACACGGT 
GATGACAAAT TCACAGTCAA AGAATTCGTC TCCTCTGTTC CCGGACCCGC ACCATTGCCT 
TTGCATGTTG AACCATCACA CCAAGTGAAT TCCCACTCGG ATAACAAAAC AAGCGTAATG 
TCTCAGAACT CAGCTCTTGA GAAGAACAAC AGTCATTCCC ATCCTCATCC CGTGGTTGAT 
GATGTCATAC ATGTTATTCG CCACAGCAGT TTCCGAGTAG GGAGCGACCA GCCGGTCATG 
GAAAGTGTTG AAGTCGGTGT CCAAAACGTC GATATGGGGA AACTCATAAA CGTTGTGAGA 
GATGAAATGG AAGTGAGAAA AGGAGCCACT CCCTCAGAAT CACCCACCAC AAGATCGATC 
ATCTCAGAGC CTGACTCAAG AACCGAGCCT CGTCCTAAAG AACCAGACCC CATCACCAAT 
TACTCAGAAA CAAAGAGCTT TAACTCCTGC TCGGATTCTT CACCAGCTGA GACCAGAACT 
AACTCATTCG TGCCTGAAGA GGAAACAACT CCGACTCCAC CTGTTAAAGA GACGTTGGAC 
ATCAAGTCGT TCAGACAGAG AGCAGAAGCG CTTGAAGGTC TATTGGAACT ATCTGCGGAT 
TTGCTGGAAC AGAGCAGGCT CGAAGAGCTA GCCATTGTGT TGCAGCCGTT TGGGAAGAAC 
AAAGTTTCGC CTCGAGAAAC CGCTATTTGG TTGGCCAAGA GTTTGAAAGG AATGATGATC 
GAAGACATCA ATAATAATAA TAGCAGTGGC AGCTCCAGGA ATTGTTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK6 ( NM_114288.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_190006.2 Arabidopsis thaliana serine/threonine-protein kinase NEK5 (NEK6), mRNA.

Price & Availability↑

CloneID	OAb12791
Clone ID Related Accession (Same CDS sequence)	NM_001339123.1 , NM_114288.4
Accession Version	NM_114288.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	serine/threonine-protein kinase NEK5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_114288.3.

ATGGAGTCAC GAATGGATCA GTACGAGCTT ATGGAACAGA TCGGGAGAGG AGCGTTTGGT 
GCTGCTATTT TAGTTCATCA TAAAGCTGAG AGAAAGAAGT ATGTTTTGAA GAAGATCAGA 
TTAGCTAGAC AAACGGAACG TTGCCGGAGA TCTGCTCATC AAGAGATGTC TTTGATTGCA 
AGAGTTCAAC ATCCTTATAT TGTGGAGTTT AAAGAAGCTT GGGTTGAGAA AGGTTGCTAT 
GTTTGTATTG TCACTGGATA CTGTGAAGGA GGAGACATGG CTGAGTTGAT GAAAAAGTCA 
AATGGTGTTT ATTTTCCTGA GGAGAAACTT TGCAAGTGGT TTACTCAATT ATTATTAGCT 
GTTGAGTATC TGCATTCTAA CTATGTTCTA CATCGGGATC TAAAGTGTTC CAACATTTTC 
CTTACAAAAG ATCAAGATGT TCGCCTTGGG GACTTTGGTC TTGCCAAAAC TTTGAAGGCG 
GATGACCTAA CTTCCTCGGT TGTTGGAACT CCAAACTACA TGTGCCCGGA ACTGCTTGCT 
GATATCCCTT ATGGTTTTAA GTCAGATATC TGGTCCTTAG GCTGTTGTAT ATATGAGATG 
GCTGCGTATC GACCTGCTTT CAAAGCTTTT GATATGGCAG GGCTTATAAG CAAGGTTAAT 
CGCTCCTCAA TTGGTCCGTT GCCTCCGTGC TATTCACCAT CTTTAAAAGC ACTCATCAAG 
GGAATGTTAA GGAAGAACCC CGAGTATCGG CCAAACGCCT CCGAGATTTT GAAGCATCCT 
TATCTGCAGC CATATGTTGA GCAGTACCGT CCAACGCTTT CTGCGGCGTC TATAACTCCA 
GAAAAGCCTC TCAATTCTCG CGAGGGTCGG AGGAGTATGG CTGAAAGTCA GAATAGCAAT 
AGTTCAAGTG AAAAAGATAA CTTCTACGTG AGTGACAAAA ATATCCGATA TGTGGTTCCA 
AGTAATGGTA ATAAAGTCAC TGAGACCGAT TCAGGTTTTG TCGATGATGA AGACATCTTA 
GACCATGTGC AACAATCAGC TGAAAATGGT AATCTCCAGA GTGTTTCAGC AACAAAACCA 
GATGGCCATG GGATTTTAAA GCCCGTACAC AGTGACCAGC GACCAGACGT TATTCAACCA 
AGGCACCCAA AAACTATCAG AAACATCATG ATGGTTTTGA AAGAAGAGAA AGCTCGAGAA 
AATGGTTCAC CCATGAGATC TAATCGAAGT AGACCTTCTA GTGTTCCGAC ACAGAAGAAT 
AATGTTGAAA CTCCATCAAA GATTCCCAAA CTTGGTGACA TTGCTCATAG CTCCAAGACT 
AACGCAAGTA CGCCTATTCC ACCATCAAAG CTGGCCTCTG ATTCCGCAAG GACTCCAGGA 
TCATTTCCAC CAAAGCATCA TATGCCAGTG ATTGACTCTT CTCCAAAACT TAAGCCTAGA 
AACGACAGAA TTTCACCTTC TCCTGCTGCT AAACATGAGG CTGAAGAGGC GATGTCAGTT 
AAGCGTAGGC AAAGAACACC TCCTACTTTG CCAAGAAGAA CGTCTTTGAT AGCGCACCAG 
TCGAGACAAC TAGGAGCAGA TATTTCAAAT ATGGCAGCAA AGGAAACCGC GAAGCTACAT 
CCATCTGTGC CATCAGAGTC TGAAACTAAT TCTCATCAAT CTCGTGTTCA TGCTTCTCCT 
GTGAGTACGA CGCCAGAGCC AAAGAGAACC TCTGTTGGAT CTGCCAAAGG AATGCAGAGT 
GAAAGCAGCA ACTCTATCTC GTCGTCGTTG TCCATGCAGG CATTTGAGCT CTGTGATGAT 
GCTTCCACCC CATATATTGA CATGACAGAA CATACAACTC CTGATGACCA CAGAAGATCC 
TGTCACAGTG AATACTCATA TTCATTTCCA GATATCTCCT CAGAGATGAT GATCCGTAGA 
GATGAACATA GCACCAGTAT GCGGCTGACT GAAATTCCCG ACTCTGTTTC CGGTGTACAA 
AACACCATTG CCCATCATCA GCCAGAAAGA GAGCAAGGAA GCTGTCCCAC TGTGCTCAAA 
GATGATAGTC CTGCAACATT ACAGAGCTAC GAGCCTAACA CATCACAACA TCAACACGGT 
GATGACAAAT TCACAGTCAA AGAATTCGTC TCCTCTGTTC CCGGACCCGC ACCATTGCCT 
TTGCATGTTG AACCATCACA CCAAGTGAAT TCCCACTCGG ATAACAAAAC AAGCGTAATG 
TCTCAGAACT CAGCTCTTGA GAAGAACAAC AGTCATTCCC ATCCTCATCC CGTGGTTGAT 
GATGTCATAC ATGTTATTCG CCACAGCAGT TTCCGAGTAG GGAGCGACCA GCCGGTCATG 
GAAAGTGTTG AAGTCGGTGT CCAAAACGTC GATATGGGGA AACTCATAAA CGTTGTGAGA 
GATGAAATGG AAGTGAGAAA AGGAGCCACT CCCTCAGAAT CACCCACCAC AAGATCGATC 
ATCTCAGAGC CTGACTCAAG AACCGAGCCT CGTCCTAAAG AACCAGACCC CATCACCAAT 
TACTCAGAAA CAAAGAGCTT TAACTCCTGC TCGGATTCTT CACCAGCTGA GACCAGAACT 
AACTCATTCG TGCCTGAAGA GGAAACAACT CCGACTCCAC CTGTTAAAGA GACGTTGGAC 
ATCAAGTCGT TCAGACAGAG AGCAGAAGCG CTTGAAGGTC TATTGGAACT ATCTGCGGAT 
TTGCTGGAAC AGAGCAGGCT CGAAGAGCTA GCCATTGTGT TGCAGCCGTT TGGGAAGAAC 
AAAGTTTCGC CTCGAGAAAC CGCTATTTGG TTGGCCAAGA GTTTGAAAGG AATGATGATC 
GAAGACATCA ATAATAATAA TAGCAGTGGC AGCTCCAGGA ATTGTTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_190006.2
CDS	441..3311
Translation	MESRMDQYELMEQIGRGAFGAAILVHHKAERKKYVLKKIRLARQTERCRRSAHQEMSLIARVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGVYFPEEKLCKWFTQLLLAVEYLHSNYVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLTSSVVGTPNYMCPELLADIPYGFKSDIWSLGCCIYEMAAYRPAFKAFDMAGLISKVNRSSIGPLPPCYSPSLKALIKGMLRKNPEYRPNASEILKHPYLQPYVEQYRPTLSAASITPEKPLNSREGRRSMAESQNSNSSSEKDNFYVSDKNIRYVVPSNGNKVTETDSGFVDDEDILDHVQQSAENGNLQSVSATKPDGHGILKPVHSDQRPDVIQPRHPKTIRNIMMVLKEEKARENGSPMRSNRSRPSSVPTQKNNVETPSKIPKLGDIAHSSKTNASTPIPPSKLASDSARTPGSFPPKHHMPVIDSSPKLKPRNDRISPSPAAKHEAEEAMSVKRRQRTPPTLPRRTSLIAHQSRQLGADISNMAAKETAKLHPSVPSESETNSHQSRVHASPVSTTPEPKRTSVGSAKGMQSESSNSISSSLSMQAFELCDDASTPYIDMTEHTTPDDHRRSCHSEYSYSFPDISSEMMIRRDEHSTSMRLTEIPDSVSGVQNTIAHHQPEREQGSCPTVLKDDSPATLQSYEPNTSQHQHGDDKFTVKEFVSSVPGPAPLPLHVEPSHQVNSHSDNKTSVMSQNSALEKNNSHSHPHPVVDDVIHVIRHSSFRVGSDQPVMESVEVGVQNVDMGKLINVVRDEMEVRKGATPSESPTTRSIISEPDSRTEPRPKEPDPITNYSETKSFNSCSDSSPAETRTNSFVPEEETTPTPPVKETLDIKSFRQRAEALEGLLELSADLLEQSRLEELAIVLQPFGKNKVSPRETAIWLAKSLKGMMIEDINNNNSSGSSRNCS

Target ORF information:

RefSeq Version	NM_114288.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana serine/threonine-protein kinase NEK5 (NEK6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_114288.4

ORF Insert Sequence:

ATGGAGTCAC GAATGGATCA GTACGAGCTT ATGGAACAGA TCGGGAGAGG AGCGTTTGGT 
GCTGCTATTT TAGTTCATCA TAAAGCTGAG AGAAAGAAGT ATGTTTTGAA GAAGATCAGA 
TTAGCTAGAC AAACGGAACG TTGCCGGAGA TCTGCTCATC AAGAGATGTC TTTGATTGCA 
AGAGTTCAAC ATCCTTATAT TGTGGAGTTT AAAGAAGCTT GGGTTGAGAA AGGTTGCTAT 
GTTTGTATTG TCACTGGATA CTGTGAAGGA GGAGACATGG CTGAGTTGAT GAAAAAGTCA 
AATGGTGTTT ATTTTCCTGA GGAGAAACTT TGCAAGTGGT TTACTCAATT ATTATTAGCT 
GTTGAGTATC TGCATTCTAA CTATGTTCTA CATCGGGATC TAAAGTGTTC CAACATTTTC 
CTTACAAAAG ATCAAGATGT TCGCCTTGGG GACTTTGGTC TTGCCAAAAC TTTGAAGGCG 
GATGACCTAA CTTCCTCGGT TGTTGGAACT CCAAACTACA TGTGCCCGGA ACTGCTTGCT 
GATATCCCTT ATGGTTTTAA GTCAGATATC TGGTCCTTAG GCTGTTGTAT ATATGAGATG 
GCTGCGTATC GACCTGCTTT CAAAGCTTTT GATATGGCAG GGCTTATAAG CAAGGTTAAT 
CGCTCCTCAA TTGGTCCGTT GCCTCCGTGC TATTCACCAT CTTTAAAAGC ACTCATCAAG 
GGAATGTTAA GGAAGAACCC CGAGTATCGG CCAAACGCCT CCGAGATTTT GAAGCATCCT 
TATCTGCAGC CATATGTTGA GCAGTACCGT CCAACGCTTT CTGCGGCGTC TATAACTCCA 
GAAAAGCCTC TCAATTCTCG CGAGGGTCGG AGGAGTATGG CTGAAAGTCA GAATAGCAAT 
AGTTCAAGTG AAAAAGATAA CTTCTACGTG AGTGACAAAA ATATCCGATA TGTGGTTCCA 
AGTAATGGTA ATAAAGTCAC TGAGACCGAT TCAGGTTTTG TCGATGATGA AGACATCTTA 
GACCATGTGC AACAATCAGC TGAAAATGGT AATCTCCAGA GTGTTTCAGC AACAAAACCA 
GATGGCCATG GGATTTTAAA GCCCGTACAC AGTGACCAGC GACCAGACGT TATTCAACCA 
AGGCACCCAA AAACTATCAG AAACATCATG ATGGTTTTGA AAGAAGAGAA AGCTCGAGAA 
AATGGTTCAC CCATGAGATC TAATCGAAGT AGACCTTCTA GTGTTCCGAC ACAGAAGAAT 
AATGTTGAAA CTCCATCAAA GATTCCCAAA CTTGGTGACA TTGCTCATAG CTCCAAGACT 
AACGCAAGTA CGCCTATTCC ACCATCAAAG CTGGCCTCTG ATTCCGCAAG GACTCCAGGA 
TCATTTCCAC CAAAGCATCA TATGCCAGTG ATTGACTCTT CTCCAAAACT TAAGCCTAGA 
AACGACAGAA TTTCACCTTC TCCTGCTGCT AAACATGAGG CTGAAGAGGC GATGTCAGTT 
AAGCGTAGGC AAAGAACACC TCCTACTTTG CCAAGAAGAA CGTCTTTGAT AGCGCACCAG 
TCGAGACAAC TAGGAGCAGA TATTTCAAAT ATGGCAGCAA AGGAAACCGC GAAGCTACAT 
CCATCTGTGC CATCAGAGTC TGAAACTAAT TCTCATCAAT CTCGTGTTCA TGCTTCTCCT 
GTGAGTACGA CGCCAGAGCC AAAGAGAACC TCTGTTGGAT CTGCCAAAGG AATGCAGAGT 
GAAAGCAGCA ACTCTATCTC GTCGTCGTTG TCCATGCAGG CATTTGAGCT CTGTGATGAT 
GCTTCCACCC CATATATTGA CATGACAGAA CATACAACTC CTGATGACCA CAGAAGATCC 
TGTCACAGTG AATACTCATA TTCATTTCCA GATATCTCCT CAGAGATGAT GATCCGTAGA 
GATGAACATA GCACCAGTAT GCGGCTGACT GAAATTCCCG ACTCTGTTTC CGGTGTACAA 
AACACCATTG CCCATCATCA GCCAGAAAGA GAGCAAGGAA GCTGTCCCAC TGTGCTCAAA 
GATGATAGTC CTGCAACATT ACAGAGCTAC GAGCCTAACA CATCACAACA TCAACACGGT 
GATGACAAAT TCACAGTCAA AGAATTCGTC TCCTCTGTTC CCGGACCCGC ACCATTGCCT 
TTGCATGTTG AACCATCACA CCAAGTGAAT TCCCACTCGG ATAACAAAAC AAGCGTAATG 
TCTCAGAACT CAGCTCTTGA GAAGAACAAC AGTCATTCCC ATCCTCATCC CGTGGTTGAT 
GATGTCATAC ATGTTATTCG CCACAGCAGT TTCCGAGTAG GGAGCGACCA GCCGGTCATG 
GAAAGTGTTG AAGTCGGTGT CCAAAACGTC GATATGGGGA AACTCATAAA CGTTGTGAGA 
GATGAAATGG AAGTGAGAAA AGGAGCCACT CCCTCAGAAT CACCCACCAC AAGATCGATC 
ATCTCAGAGC CTGACTCAAG AACCGAGCCT CGTCCTAAAG AACCAGACCC CATCACCAAT 
TACTCAGAAA CAAAGAGCTT TAACTCCTGC TCGGATTCTT CACCAGCTGA GACCAGAACT 
AACTCATTCG TGCCTGAAGA GGAAACAACT CCGACTCCAC CTGTTAAAGA GACGTTGGAC 
ATCAAGTCGT TCAGACAGAG AGCAGAAGCG CTTGAAGGTC TATTGGAACT ATCTGCGGAT 
TTGCTGGAAC AGAGCAGGCT CGAAGAGCTA GCCATTGTGT TGCAGCCGTT TGGGAAGAAC 
AAAGTTTCGC CTCGAGAAAC CGCTATTTGG TTGGCCAAGA GTTTGAAAGG AATGATGATC 
GAAGACATCA ATAATAATAA TAGCAGTGGC AGCTCCAGGA ATTGTTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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NEK6 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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