DCL3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	DCL3
Entrez Gene ID	823508
Full Name	dicer-like 3
Synonyms	ATDCL3,DICER-LIKE 3,dicer-like 3
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001339116.1	NP_001325813.1	dicer-like 3
NM_001161191.3	NP_001154663.2	dicer-like 3
NM_001339114.1	NP_001325812.1	dicer-like 3
NM_114260.1	NP_189978.1	dicer-like 3
NM_001161190.2	NP_001154662.2	dicer-like 3
NM_001339115.1	NP_001325811.1	dicer-like 3

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os01g0909200	NP_001045148.1
Arabidopsis thaliana (thale cress)	DCL3	NP_001154662.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data demonstrate that different nutrient statuses of a host plant affect the post-translational regulation of the dicing activity of DCL3 and DCL4. Phosphate deficiency inhibited DCL3, and the activity of DCL3 was directly activated by inorganic phosphate.
Title: Post-Translational Regulation of the Dicing Activities of Arabidopsis DICER-LIKE 3 and 4 by Inorganic Phosphate and the Redox State.

This expression-dependent RdDM mechanism functions through RNAi degradation of transposable element mRNAs into small RNAs guided by the RNA-dependent RNA polymerase 6 (RDR6) protein and is therefore referred to as RDR6-RdDM.We identified a new mechanism of expression-dependent RdDM, which functions through DICER-LIKE3 (DCL3) but bypasses the requirement of both RNA polymerase IV and RDR6 (termed DCL3-RdDM).
Title: Full-length autonomous transposable elements are preferentially targeted by expression-dependent forms of RNA-directed DNA methylation.

The authors identified Pol IV and RDR2-dependent RNAs (P4R2 RNAs) that accumulate in dcl3 mutants and are diced into 24 nt RNAs by DCL3 in vitro.
Title: Identification of Pol IV and RDR2-dependent precursors of 24 nt siRNAs guiding de novo DNA methylation in Arabidopsis.

CMA33/XCT modulates small RNA production through regulating the transcription of Dicer-Like Genes.
Title: CMA33/XCT Regulates Small RNA Production through Modulating the Transcription of Dicer-Like Genes in Arabidopsis.

DCL3 preferentially cleaves short dsRNAs with 5' phosphorylated adenosine or uridine and a 1 nt 3' overhang.
Title: Distinct substrate specificities of Arabidopsis DCL3 and DCL4.

The following DCL3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCL3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb37991	NM_001339116.1 Latest version!	Arabidopsis thaliana dicer-like 3 (DCL3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OAb37989	NM_001339115.1 Latest version!	Arabidopsis thaliana dicer-like 3 (DCL3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAb37990	NM_001339114.1 Latest version!	Arabidopsis thaliana dicer-like 3 (DCL3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAb12769	NM_114260.1 Latest version!	Arabidopsis thaliana dicer-like 3 (DCL3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OAb29531	NM_001161191.3 Latest version!	Arabidopsis thaliana dicer-like 3 (DCL3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OAb29532	NM_001161190.2 Latest version!	Arabidopsis thaliana dicer-like 3 (DCL3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DCL3 ( NM_001339116.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001325813.1 Arabidopsis thaliana dicer-like 3 (DCL3), partial mRNA.

Price & Availability↑

CloneID	OAb37991
Clone ID Related Accession (Same CDS sequence)	NM_001339116.1
Accession Version	NM_001339116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	dicer-like 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). COMPLETENESS: incomplete on the 3' end.

ATGGACTCAA AGATTTTTAA TCCTGAAGAG CGTGAAGGAG TGGAAAAGTT TGCTACAACG 
GTTAAAGAAG GTCCAATATT GTATAACCCA TCACCATCCT GTAGTTTGGA ATTGAAAGAA 
AAGTTAGAAA CTTCACACCT CAAGTTTGAT GCTTCTCTTA GAAGGCTTCA AGAGTTGGGA 
AAAGACAGTT TTCTGAATAT GGATAATAAG TTTGAGACAT ATCAAAAGAG ATTGTCTATC 
GACTACAGAG AGATTTTGCA TTGCCTTGAT AATCTTGGCC TGATTTGCGC ACACTTGGCG 
GCTGAAGTCT GCTTGGAGAA AATCTCAGAT ACGAAAGAGG AAAGTGAAAC TTATAAAGAA 
TGCTCAATGG TGTGCAAGGA ATTTCTTGAG GATATTTTAT CCACCATTGG GGTGTATTTG 
CCGCAAGATG ATAAGAGTCT GGTAGATTTG CAGCAAAACC ATCTGTCAGC AGTAATTTCT 
GGGCATGTAT CTCCAAAGCT AAAAGAACTC TTCCATCTAT TGGATTCCTT TAGAGGTGAC 
AAGCAAAAGC AGTGCCTTAT TTTAGTTGAG AGAATTATAA CTGCGAAAGT GATCGAAAGA 
TTCGTTAAGA AAGAAGCCTC TTTGGCTTAC CTTAATGTCT TGTATTTAAC CGAAAACAAC 
CCCTCCACCA ATGTATCGGC ACAGAAAATG CAAATTGAAA TCCCTGATTT ATTTCAACAT 
GGCAAGGTGA ATCTTTTATT CATCACAGAT GTGGTTGAAG AGGGATTTCA GGTTCCAGAT 
TGCTCATGCA TGGTTTGTTT TGACCTGCCC AAAACAATGT GTAGTTACTC GCAGTCTCAA 
AAACATGCCA AACAGAGTAA TTCTAAGTCT ATCATGTTTC TTGAAAGAGG GAACCCGAAG 
CAAAGAGACC ATCTGCATGA CCTTATGCGA AGAGAAGTCC TAATTCAAGA TCCAGAAGCT 
CCAAACTTGA AATCGTGTCC ACCTCCAGTG AAAAATGGAC ACGGTGTGAA GGAGATTGGA 
TCCATGGTTA TCCCAGATTC TAACATAACT GTATCTGAGG AAGCAGCTTC CACACAAACT 
ATGAGTGATC CTCCTAGCAG AAATGAGCAG TTACCACCGT GTAAAAAGTT ACGCTTGGAT 
AACAATCTCT TACAATCCAA CGGCAAAGAG AAGGTTGCCT CTTCTAAAAG TAAATCATCT 
TCATCGGCTG CAGGTTCAAA AAAACGTAAG GAGTTGCACG GAACAACCTG TGCAAACGCA 
TTGTCAGGAA CCTGGGGAGA AAATATTGAT GGCGCCACCT TTCAGGCTTA TAAGTTTGAC 
TTCTGTTGTA ATATTTCTGG CGAAGTATAC TCGAGTTTCT CTCTTTTGCT TGAGTCAACT 
CTCGCCGAGG ATGTTGGTAA AGTTGAGATG GACCTTTACT TGGTCAGGAA GCTTGTCAAG 
GCTTCTGTCT CACCTTGTGG CCAGATACGT TTGAGTCAAG AGGAGCTGGT CAAAGCAAAA 
TATTTTCAGC AGTTTTTCTT TAATGGCATG TTTGGAAAGT TGTTTGTTGG ATCTAAGTCA 
CAGGGAACAA AGAGAGAATT TTTGCTTCAA ACTGACACTA GTTCTCTTTG GCACCCTGCC 
TTTATGTTTC TACTGCTACC AGTTGAAACA AATGATCTAG CTTCGAGTGC GACAATTGAT 
TGGTCAGCTA TCAACTCCTG TGCCTCAATA GTTGAGTTCT TGAAGAAAAA TTCTCTTCTT 
GATCTTCGGG ATAGTGATGG GAATCAGTGC AATACCTCAT CCGGTCAGGA AGTCTTACTA 
GACGATAAAA TGGAAGAAAC GAATCTGATT CATTTTGCCA ATGCTTCGTC TGATAAAAAT 
AGTCTCGAAG AACTTGTGGT CATTGCAATT CATACTGGAC GGATATACTC TATAGTTGAA 
GCCGTAAGCG ATTCTTCTGC TATGAGCCCC TTTGAGGTGG ATGCCTCATC AGGCTATGCT 
ACTTATGCAG AATATTTTAA CAAAAAGTAT GGGATTGTTT TAGCGCACCC GAACCAGCCG 
TTGATGAAGT TGAAGCAGAG TCACCATGCG CACAACCTTT TAGTCGACTT CAATGAAGAG 
ATGGTTGTGA AGACAGAACC AAAAGCTGGC AATGTTAGGA AAAGAAAACC GAATATCCAT 
GCGCATTTGC CTCCAGAGCT TTTGGCTAGA ATTGATGTAC CGCGTGCTGT GCTAAAATCA 
ATCTACTTGC TGCCTTCAGT GATGCACCGC CTAGAGTCTC TAATGTTGGC CAGCCAGCTT 
AGGGAAGAGA TTGATTGTAG CATAGATAAC TTCAGTATAT CAAGTACATC GATTCTTGAA 
GCAGTTACAA CACTTACATG CCCCGAATCA TTTTCAATGG AGCGGTTGGA ACTGCTCGGG 
GATTCAGTCT TGAAGTATGT TGCGAGCTGT CATCTATTCC TTAAGTATCC TGACAAAGAT 
GAGGGGCAAC TATCACGGCA GAGACAATCG ATTATATCTA ACTCAAATCT TCACCGCTTG 
ACAACCAGTC GCAAACTACA GGGATACATA AGAAATGGCG CTTTTGAACC GCGTCGCTGG 
ACTGCACCTG GTCAATTTTC TCTTTTTCCT GTTCCTTGCA AGTGTGGGAT TGATACTAGA 
GAAGTACCAT TGGACCCAAA ATTCTTCACA GAAAACATGA CTATCAAAAT AGGCAAGTCT 
TGCGACATGG GTCATAGATG GGTAGTTTCA AAATCTGTAT CAGATTGCGC TGAGGCCCTG 
ATTGGTGCCT ATTATGTAAG CGGTGGATTG TCTGCTTCTC TCCATATGAT GAAATGGCTC 
GGTATTGACG TCGATTTTGA CCCAAACCTA GTCGTTGAAG CCATCAATAG AGTTTCTCTA 
CGGTGTTACA TTCCTAAAGA AGATGAGCTC ATAGAGTTGG AGAGAAAGAT CCAACATGAA 
TTCTCTGCAA AGTTTCTTTT AAAAGAGGCT ATCACACACT CCTCTCTTCG TGAATCCTAT 
TCATACGAGA GATTAGAGTT TCTTGGCGAT TCTGTACTGG ATTTTCTAAT AACCCGTCAT 
CTTTTTAACA CCTACGAACA AACTGGGCCT GGAGAGATGA CCGATCTTCG TTCTGCATGT 
GTAAACAATG AAAATTTTGC GCAAGTTGCA GTGAAAAATA ACCTGCATAC CCACCTTCAA 
CGCTGTGCTA CGGTTCTCGA GACTCAAATA AACGACTATC TGATGTCCTT TCAAAAGCCA 
GATGAGACTG GTAGATCAAT CCCTTCAATA CAGGGCCCTA AGGCTCTTGG AGATGTTGTG 
GAGAGTATCG CTGGAGCATT GCTGATCGAT ACGAGGTTAG ATCTCGATCA AGTGTGGAGA 
GTCTTTGAGC CGTTGCTTTC TCCACTTGTA ACTCCAGATA AACTTCAGCT TCCTCCATAC 
CGGGAGCTCA ATGAGCTATG CGACTCTCTT GGGTATTTCT TTCGAGTGAA ATGTTCAAAT 
GATGGTGTCA AAGCACAAGC CACGATCCAG TTGCAGCTGG ATGATGTTCT TTTAACTGGA 
GATGGATCTG AACAGACAAA TAAACTGGCC TTGGGAAAAG CAGCTTCACA TCTGCTTACA 
CAACTTGAGA AGAGAAACAT TTCACGTAAA ACCTCGCTCG GGGATAATCA AAGTTCCATG 
GATGTCAATC TTGCTTGCAA TCATAGCGAC AGAGAAACTC TGACTTCAGA GACTACTGAA 
ATCCAGAGTA TAGTGATTCC ATTTATTGGA CCTATAAACA TGAAGAAAGG CGGGCCTCGT 
GGAACTCTAC ATGAGTTTTG CAAGAAGCAT CTGTGGCCAA TGCCTACTTT CGATACCTCG 
GAAGAGAAAT CCAGAACTCC GTTTGAATTC ATAGATGGCG GTGAGAAGCG GACTAGCTTC 
AGCAGTTTCA CATCGACCAT AACCCTAAGG ATACCCAATC GTGAGGCTGT GATGTATGCT 
GGAGAAGCAA GGCCTGACAA GAAGAGTTCC TTCGACTCTG CAGTCGTGGA ATTGCTTTAT 
GAGCTCGAGC GCCGCAAGAT CGTCATAATA CAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001325813.1
CDS	186..4244
Translation	MDSKIFNPEEREGVEKFATTVKEGPILYNPSPSCSLELKEKLETSHLKFDASLRRLQELGKDSFLNMDNKFETYQKRLSIDYREILHCLDNLGLICAHLAAEVCLEKISDTKEESETYKECSMVCKEFLEDILSTIGVYLPQDDKSLVDLQQNHLSAVISGHVSPKLKELFHLLDSFRGDKQKQCLILVERIITAKVIERFVKKEASLAYLNVLYLTENNPSTNVSAQKMQIEIPDLFQHGKVNLLFITDVVEEGFQVPDCSCMVCFDLPKTMCSYSQSQKHAKQSNSKSIMFLERGNPKQRDHLHDLMRREVLIQDPEAPNLKSCPPPVKNGHGVKEIGSMVIPDSNITVSEEAASTQTMSDPPSRNEQLPPCKKLRLDNNLLQSNGKEKVASSKSKSSSSAAGSKKRKELHGTTCANALSGTWGENIDGATFQAYKFDFCCNISGEVYSSFSLLLESTLAEDVGKVEMDLYLVRKLVKASVSPCGQIRLSQEELVKAKYFQQFFFNGMFGKLFVGSKSQGTKREFLLQTDTSSLWHPAFMFLLLPVETNDLASSATIDWSAINSCASIVEFLKKNSLLDLRDSDGNQCNTSSGQEVLLDDKMEETNLIHFANASSDKNSLEELVVIAIHTGRIYSIVEAVSDSSAMSPFEVDASSGYATYAEYFNKKYGIVLAHPNQPLMKLKQSHHAHNLLVDFNEEMVVKTEPKAGNVRKRKPNIHAHLPPELLARIDVPRAVLKSIYLLPSVMHRLESLMLASQLREEIDCSIDNFSISSTSILEAVTTLTCPESFSMERLELLGDSVLKYVASCHLFLKYPDKDEGQLSRQRQSIISNSNLHRLTTSRKLQGYIRNGAFEPRRWTAPGQFSLFPVPCKCGIDTREVPLDPKFFTENMTIKIGKSCDMGHRWVVSKSVSDCAEALIGAYYVSGGLSASLHMMKWLGIDVDFDPNLVVEAINRVSLRCYIPKEDELIELERKIQHEFSAKFLLKEAITHSSLRESYSYERLEFLGDSVLDFLITRHLFNTYEQTGPGEMTDLRSACVNNENFAQVAVKNNLHTHLQRCATVLETQINDYLMSFQKPDETGRSIPSIQGPKALGDVVESIAGALLIDTRLDLDQVWRVFEPLLSPLVTPDKLQLPPYRELNELCDSLGYFFRVKCSNDGVKAQATIQLQLDDVLLTGDGSEQTNKLALGKAASHLLTQLEKRNISRKTSLGDNQSSMDVNLACNHSDRETLTSETTEIQSIVIPFIGPINMKKGGPRGTLHEFCKKHLWPMPTFDTSEEKSRTPFEFIDGGEKRTSFSSFTSTITLRIPNREAVMYAGEARPDKKSSFDSAVVELLYELERRKIVIIQK

Target ORF information:

RefSeq Version	NM_001339116.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana dicer-like 3 (DCL3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001339116.1

ORF Insert Sequence:

ATGGACTCAA AGATTTTTAA TCCTGAAGAG CGTGAAGGAG TGGAAAAGTT TGCTACAACG 
GTTAAAGAAG GTCCAATATT GTATAACCCA TCACCATCCT GTAGTTTGGA ATTGAAAGAA 
AAGTTAGAAA CTTCACACCT CAAGTTTGAT GCTTCTCTTA GAAGGCTTCA AGAGTTGGGA 
AAAGACAGTT TTCTGAATAT GGATAATAAG TTTGAGACAT ATCAAAAGAG ATTGTCTATC 
GACTACAGAG AGATTTTGCA TTGCCTTGAT AATCTTGGCC TGATTTGCGC ACACTTGGCG 
GCTGAAGTCT GCTTGGAGAA AATCTCAGAT ACGAAAGAGG AAAGTGAAAC TTATAAAGAA 
TGCTCAATGG TGTGCAAGGA ATTTCTTGAG GATATTTTAT CCACCATTGG GGTGTATTTG 
CCGCAAGATG ATAAGAGTCT GGTAGATTTG CAGCAAAACC ATCTGTCAGC AGTAATTTCT 
GGGCATGTAT CTCCAAAGCT AAAAGAACTC TTCCATCTAT TGGATTCCTT TAGAGGTGAC 
AAGCAAAAGC AGTGCCTTAT TTTAGTTGAG AGAATTATAA CTGCGAAAGT GATCGAAAGA 
TTCGTTAAGA AAGAAGCCTC TTTGGCTTAC CTTAATGTCT TGTATTTAAC CGAAAACAAC 
CCCTCCACCA ATGTATCGGC ACAGAAAATG CAAATTGAAA TCCCTGATTT ATTTCAACAT 
GGCAAGGTGA ATCTTTTATT CATCACAGAT GTGGTTGAAG AGGGATTTCA GGTTCCAGAT 
TGCTCATGCA TGGTTTGTTT TGACCTGCCC AAAACAATGT GTAGTTACTC GCAGTCTCAA 
AAACATGCCA AACAGAGTAA TTCTAAGTCT ATCATGTTTC TTGAAAGAGG GAACCCGAAG 
CAAAGAGACC ATCTGCATGA CCTTATGCGA AGAGAAGTCC TAATTCAAGA TCCAGAAGCT 
CCAAACTTGA AATCGTGTCC ACCTCCAGTG AAAAATGGAC ACGGTGTGAA GGAGATTGGA 
TCCATGGTTA TCCCAGATTC TAACATAACT GTATCTGAGG AAGCAGCTTC CACACAAACT 
ATGAGTGATC CTCCTAGCAG AAATGAGCAG TTACCACCGT GTAAAAAGTT ACGCTTGGAT 
AACAATCTCT TACAATCCAA CGGCAAAGAG AAGGTTGCCT CTTCTAAAAG TAAATCATCT 
TCATCGGCTG CAGGTTCAAA AAAACGTAAG GAGTTGCACG GAACAACCTG TGCAAACGCA 
TTGTCAGGAA CCTGGGGAGA AAATATTGAT GGCGCCACCT TTCAGGCTTA TAAGTTTGAC 
TTCTGTTGTA ATATTTCTGG CGAAGTATAC TCGAGTTTCT CTCTTTTGCT TGAGTCAACT 
CTCGCCGAGG ATGTTGGTAA AGTTGAGATG GACCTTTACT TGGTCAGGAA GCTTGTCAAG 
GCTTCTGTCT CACCTTGTGG CCAGATACGT TTGAGTCAAG AGGAGCTGGT CAAAGCAAAA 
TATTTTCAGC AGTTTTTCTT TAATGGCATG TTTGGAAAGT TGTTTGTTGG ATCTAAGTCA 
CAGGGAACAA AGAGAGAATT TTTGCTTCAA ACTGACACTA GTTCTCTTTG GCACCCTGCC 
TTTATGTTTC TACTGCTACC AGTTGAAACA AATGATCTAG CTTCGAGTGC GACAATTGAT 
TGGTCAGCTA TCAACTCCTG TGCCTCAATA GTTGAGTTCT TGAAGAAAAA TTCTCTTCTT 
GATCTTCGGG ATAGTGATGG GAATCAGTGC AATACCTCAT CCGGTCAGGA AGTCTTACTA 
GACGATAAAA TGGAAGAAAC GAATCTGATT CATTTTGCCA ATGCTTCGTC TGATAAAAAT 
AGTCTCGAAG AACTTGTGGT CATTGCAATT CATACTGGAC GGATATACTC TATAGTTGAA 
GCCGTAAGCG ATTCTTCTGC TATGAGCCCC TTTGAGGTGG ATGCCTCATC AGGCTATGCT 
ACTTATGCAG AATATTTTAA CAAAAAGTAT GGGATTGTTT TAGCGCACCC GAACCAGCCG 
TTGATGAAGT TGAAGCAGAG TCACCATGCG CACAACCTTT TAGTCGACTT CAATGAAGAG 
ATGGTTGTGA AGACAGAACC AAAAGCTGGC AATGTTAGGA AAAGAAAACC GAATATCCAT 
GCGCATTTGC CTCCAGAGCT TTTGGCTAGA ATTGATGTAC CGCGTGCTGT GCTAAAATCA 
ATCTACTTGC TGCCTTCAGT GATGCACCGC CTAGAGTCTC TAATGTTGGC CAGCCAGCTT 
AGGGAAGAGA TTGATTGTAG CATAGATAAC TTCAGTATAT CAAGTACATC GATTCTTGAA 
GCAGTTACAA CACTTACATG CCCCGAATCA TTTTCAATGG AGCGGTTGGA ACTGCTCGGG 
GATTCAGTCT TGAAGTATGT TGCGAGCTGT CATCTATTCC TTAAGTATCC TGACAAAGAT 
GAGGGGCAAC TATCACGGCA GAGACAATCG ATTATATCTA ACTCAAATCT TCACCGCTTG 
ACAACCAGTC GCAAACTACA GGGATACATA AGAAATGGCG CTTTTGAACC GCGTCGCTGG 
ACTGCACCTG GTCAATTTTC TCTTTTTCCT GTTCCTTGCA AGTGTGGGAT TGATACTAGA 
GAAGTACCAT TGGACCCAAA ATTCTTCACA GAAAACATGA CTATCAAAAT AGGCAAGTCT 
TGCGACATGG GTCATAGATG GGTAGTTTCA AAATCTGTAT CAGATTGCGC TGAGGCCCTG 
ATTGGTGCCT ATTATGTAAG CGGTGGATTG TCTGCTTCTC TCCATATGAT GAAATGGCTC 
GGTATTGACG TCGATTTTGA CCCAAACCTA GTCGTTGAAG CCATCAATAG AGTTTCTCTA 
CGGTGTTACA TTCCTAAAGA AGATGAGCTC ATAGAGTTGG AGAGAAAGAT CCAACATGAA 
TTCTCTGCAA AGTTTCTTTT AAAAGAGGCT ATCACACACT CCTCTCTTCG TGAATCCTAT 
TCATACGAGA GATTAGAGTT TCTTGGCGAT TCTGTACTGG ATTTTCTAAT AACCCGTCAT 
CTTTTTAACA CCTACGAACA AACTGGGCCT GGAGAGATGA CCGATCTTCG TTCTGCATGT 
GTAAACAATG AAAATTTTGC GCAAGTTGCA GTGAAAAATA ACCTGCATAC CCACCTTCAA 
CGCTGTGCTA CGGTTCTCGA GACTCAAATA AACGACTATC TGATGTCCTT TCAAAAGCCA 
GATGAGACTG GTAGATCAAT CCCTTCAATA CAGGGCCCTA AGGCTCTTGG AGATGTTGTG 
GAGAGTATCG CTGGAGCATT GCTGATCGAT ACGAGGTTAG ATCTCGATCA AGTGTGGAGA 
GTCTTTGAGC CGTTGCTTTC TCCACTTGTA ACTCCAGATA AACTTCAGCT TCCTCCATAC 
CGGGAGCTCA ATGAGCTATG CGACTCTCTT GGGTATTTCT TTCGAGTGAA ATGTTCAAAT 
GATGGTGTCA AAGCACAAGC CACGATCCAG TTGCAGCTGG ATGATGTTCT TTTAACTGGA 
GATGGATCTG AACAGACAAA TAAACTGGCC TTGGGAAAAG CAGCTTCACA TCTGCTTACA 
CAACTTGAGA AGAGAAACAT TTCACGTAAA ACCTCGCTCG GGGATAATCA AAGTTCCATG 
GATGTCAATC TTGCTTGCAA TCATAGCGAC AGAGAAACTC TGACTTCAGA GACTACTGAA 
ATCCAGAGTA TAGTGATTCC ATTTATTGGA CCTATAAACA TGAAGAAAGG CGGGCCTCGT 
GGAACTCTAC ATGAGTTTTG CAAGAAGCAT CTGTGGCCAA TGCCTACTTT CGATACCTCG 
GAAGAGAAAT CCAGAACTCC GTTTGAATTC ATAGATGGCG GTGAGAAGCG GACTAGCTTC 
AGCAGTTTCA CATCGACCAT AACCCTAAGG ATACCCAATC GTGAGGCTGT GATGTATGCT 
GGAGAAGCAA GGCCTGACAA GAAGAGTTCC TTCGACTCTG CAGTCGTGGA ATTGCTTTAT 
GAGCTCGAGC GCCGCAAGAT CGTCATAATA CAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCL3 ( NM_001339115.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001325811.1 Arabidopsis thaliana dicer-like 3 (DCL3), partial mRNA.

Price & Availability↑

CloneID	OAb37989
Clone ID Related Accession (Same CDS sequence)	NM_001339115.1
Accession Version	NM_001339115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	dicer-like 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). COMPLETENESS: incomplete on the 3' end.

ATGCATTCGT CGTTGGAGCC GGAGAAAATG GAGGAAGGTG GGGGAAGCAA TTCGCTTAAG 
AGAAAATTCT CTGAAATCGA TGGAGATCAA AATCTTGATT CTGTCTCTTC TCCTATGATG 
ACTGACTCTA ATGGTAGTTA TGAATTGAAA GTGTACGAGG TTGCTAAGAA CAGGAACATA 
ATTGCTGTTT TGGGGACAGG GATTGATAAG TCAGAGATCA CTAAGAGGCT TATCAAAGCT 
ATGGGTTCTT CTGATACAGA CAAAAGATTG ATAATTTTCT TGGCCCCAAC TGTGAATCTT 
GTTAAACAGC AATGCTGTGA GATCAGAGCA CTTGTGAATT TGAAAGTTGA AGAGTACTTT 
GGAGCTAAAG GAGTTGATAA ATGGACATCT CAGCGCTGGG ATGAGGAATT TAGCAAGCAC 
GATGTTTTAG TTATGACTCC TCAAATATTA TTGGATGTCC TTAGAAGTGC ATTCCTGAAA 
CTAGAGATGG TATGTCTTCT AATAATAGAT GAATGCCACC ATACCACTGG CAATCATCCC 
TATGCGAAGT TAATGAAGGA ATTCTATCAC GAATCCACTA GCAAACCGAA GATATTTGGA 
TTGACTGCGT CAGCCGTCAT TAGAAAAGCT CAAGTATCAG AACTTGAGAG ACTCATGGAC 
TCAAAGATTT TTAATCCTGA AGAGCGTGAA GGAGTGGAAA AGTTTGCTAC AACGGTTAAA 
GAAGGTCCAA TATTGTATAA CCCATCACCA TCCTGTAGTT TGGAATTGAA AGAAAAGTTA 
GAAACTTCAC ACCTCAAGTT TGATGCTTCT CTTAGAAGGC TTCAAGAGTT GGGAAAAGAC 
AGTTTTCTGA ATATGGATAA TAAGTTTGAG ACATATCAAA AGAGATTGTC TATCGACTAC 
AGAGAGATTT TGCATTGCCT TGATAATCTT GGCCTGATTT GCGCACACTT GGCGGCTGAA 
GTCTGCTTGG AGAAAATCTC AGATACGAAA GAGGAAAGTG AAACTTATAA AGAATGCTCA 
ATGGTGTGCA AGGAATTTCT TGAGGATATT TTATCCACCA TTGGGGTGTA TTTGCCGCAA 
GATGATAAGA GTCTGGTAGA TTTGCAGCAA AACCATCTGT CAGCAGTAAT TTCTGGGCAT 
GTATCTCCAA AGCTAAAAGA ACTCTTCCAT CTATTGGATT CCTTTAGAGG TGACAAGCAA 
AAGCAGTGCC TTATTTTAGT TGAGAGAATT ATAACTGCGA AAGTGATCGA AAGATTCGTT 
AAGAAAGAAG CCTCTTTGGC TTACCTTAAT GTCTTGTATT TAACCGAAAA CAACCCCTCC 
ACCAATGTAT CGGCACAGAA AATGCAAATT GAAATCCCTG ATTTATTTCA ACATGGCAAG 
GTGAATCTTT TATTCATCAC AGATGTGGTT GAAGAGGGAT TTCAGGTTCC AGATTGCTCA 
TGCATGGTTT GTTTTGACCT GCCCAAAACA ATGTGTAGTT ACTCGCAGTC TCAAAAACAT 
GCCAAACAGA GTAATTCTAA GTCTATCATG TTTCTTGAAA GAGGGAACCC GAAGCAAAGA 
GACCATCTGC ATGACCTTAT GCGAAGAGAA GTCCTAATTC AAGATCCAGA AGCTCCAAAC 
TTGAAATCGT GTCCACCTCC AGTGAAAAAT GGACACGGTG TGAAGGAGAT TGGATCCATG 
GTTATCCCAG ATTCTAACAT AACTGTATCT GAGGAAGCAG CTTCCACACA AACTATGAGT 
GATCCTCCTA GCAGAAATGA GCAGTTACCA CCGTGTAAAA AGTTACGCTT GGATAACAAT 
CTCTTACAAT CCAACGGCAA AGAGAAGGTT GCCTCTTCTA AAAGTAAATC ATCTTCATCG 
GCTGCAGGTT CAAAAAAACG TAAGGAGTTG CACGGAACAA CCTGTGCAAA CGCATTGTCA 
GGAACCTGGG GAGAAAATAT TGATGGCGCC ACCTTTCAGG CTTATAAGTT TGACTTCTGT 
TGTAATATTT CTGGCGAAGT ATACTCGAGT TTCTCTCTTT TGCTTGAGTC AACTCTCGCC 
GAGGATGTTG GTAAAGTTGA GATGGACCTT TACTTGGTCA GGAAGCTTGT CAAGGCTTCT 
GTCTCACCTT GTGGCCAGAT ACGTTTGAGT CAAGAGGAGC TGGTCAAAGC AAAATATTTT 
CAGCAGTTTT TCTTTAATGG CATGTTTGGA AAGTTGTTTG TTGGATCTAA GTCACAGGGA 
ACAAAGAGAG AATTTTTGCT TCAAACTGAC ACTAGTTCTC TTTGGCACCC TGCCTTTATG 
TTTCTACTGC TACCAGTTGA AACAAATGAT CTAGCTTCGA GTGCGACAAT TGATTGGTCA 
GCTATCAACT CCTGTGCCTC AATAGTTGAG TTCTTGAAGA AAAATTCTCT TCTTGATCTT 
CGGGATAGTG ATGGGAATCA GTGCAATACC TCATCCGGTC AGGAAGTCTT ACTAGACGAT 
AAAATGGAAG AAACGAATCT GATTCATTTT GCCAATGCTT CGTCTGATAA AAATAGTCTC 
GAAGAACTTG TGGTCATTGC AATTCATACT GGACGGATAT ACTCTATAGT TGAAGCCGTA 
AGCGATTCTT CTGCTATGAG CCCCTTTGAG GTGGATGCCT CATCAGGCTA TGCTACTTAT 
GCAGAATATT TTAACAAAAA GTATGGGATT GTTTTAGCGC ACCCGAACCA GCCGTTGATG 
AAGTTGAAGC AGAGTCACCA TGCGCACAAC CTTTTAGTCG ACTTCAATGA AGAGATGGTT 
GTGAAGACAG AACCAAAAGC TGGCAATGTT AGGAAAAGAA AACCGAATAT CCATGCGCAT 
TTGCCTCCAG AGCTTTTGGC TAGAATTGAT GTACCGCGTG CTGTGCTAAA ATCAATCTAC 
TTGCTGCCTT CAGTGATGCA CCGCCTAGAG TCTCTAATGT TGGCCAGCCA GCTTAGGGAA 
GAGATTGATT GTAGCATAGA TAACTTCAGT ATATCAAGTA CATCGATTCT TGAAGCAGTT 
ACAACACTTA CATGCCCCGA ATCATTTTCA ATGGAGCGGT TGGAACTGCT CGGGGATTCA 
GTCTTGAAGT ATGTTGCGAG CTGTCATCTA TTCCTTAAGT ATCCTGACAA AGATGAGGGG 
CAACTATCAC GGCAGAGACA ATCGATTATA TCTAACTCAA ATCTTCACCG CTTGACAACC 
AGTCGCAAAC TACAGGGATA CATAAGAAAT GGCGCTTTTG AACCGCGTCG CTGGACTGCA 
CCTGGTCAAT TTTCTCTTTT TCCTGTTCCT TGCAAGTGTG GGATTGATAC TAGAGAAGTA 
CCATTGGACC CAAAATTCTT CACAGAAAAC ATGACTATCA AAATAGGCAA GTCTTGCGAC 
ATGGGTCATA GATGGGTAGT TTCAAAATCT GTATCAGATT GCGCTGAGGC CCTGATTGGT 
GCCTATTATG TAAGCGGTGG ATTGTCTGCT TCTCTCCATA TGATGAAATG GCTCGGTATT 
GACGTCGATT TTGACCCAAA CCTAGTCGTT GAAGCCATCA ATAGAGTTTC TCTACGGTGT 
TACATTCCTA AAGAAGATGA GCTCATAGAG TTGGAGAGAA AGATCCAACA TGAATTCTCT 
GCAAAGTTTC TTTTAAAAGA GGCTATCACA CACTCCTCTC TTCGTGAATC CTATTCATAC 
GAGAGATTAG AGTTTCTTGG CGATTCTGTA CTGGATTTTC TAATAACCCG TCATCTTTTT 
AACACCTACG AACAAACTGG GCCTGGAGAG ATGACCGATC TTCGTTCTGC ATGTGTAAAC 
AATGAAAATT TTGCGCAAGT TGCAGTGAAA AATAACCTGC ATACCCACCT TCAACGCTGT 
GCTACGGTTC TCGAGACTCA AATAAACGAC TATCTGATGT CCTTTCAAAA GCCAGATGAG 
ACTGGTAGAT CAATCCCTTC AATACAGGGC CCTAAGGCTC TTGGAGATGT TGTGGAGAGT 
ATCGCTGGAG CATTGCTGAT CGATACGAGG TTAGATCTCG ATCAAGTGTG GAGAGTCTTT 
GAGCCGTTGC TTTCTCCACT TGTAACTCCA GATAAACTTC AGCTTCCTCC ATACCGGGAG 
CTCAATGAGC TATGCGACTC TCTTGGGTAT TTCTTTCGAG TGAAATGTTC AAATGATGGT 
GTCAAAGCAC AAGCCACGAT CCAGTTGCAG CTGGATGATG TTCTTTTAAC TGGAGATGGA 
TCTGAACAGA CAAATAAACT GGCCTTGGGA AAAGCAGCTT CACATCTGCT TACACAACTT 
GAGAAGAGAA ACATTTCACG TAAAACCTCG CTCGGGGATA ATCAAAGTTC CATGGATGTC 
AATCTTGCTT GCAATCATAG CGACAGAGAA ACTCTGACTT CAGAGACTAC TGAAATCCAG 
AGTATAGTGA TTCCATTTAT TGGACCTATA AACATGAAGA AAGGCGGGCC TCGTGGAACT 
CTACATGAGT TTTGCAAGAA GCATCTGTGG CCAATGCCTA CTTTCGATAC CTCGGAAGAG 
AAATCCAGAT GGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001325811.1
CDS	162..4679
Translation	MHSSLEPEKMEEGGGSNSLKRKFSEIDGDQNLDSVSSPMMTDSNGSYELKVYEVAKNRNIIAVLGTGIDKSEITKRLIKAMGSSDTDKRLIIFLAPTVNLVKQQCCEIRALVNLKVEEYFGAKGVDKWTSQRWDEEFSKHDVLVMTPQILLDVLRSAFLKLEMVCLLIIDECHHTTGNHPYAKLMKEFYHESTSKPKIFGLTASAVIRKAQVSELERLMDSKIFNPEEREGVEKFATTVKEGPILYNPSPSCSLELKEKLETSHLKFDASLRRLQELGKDSFLNMDNKFETYQKRLSIDYREILHCLDNLGLICAHLAAEVCLEKISDTKEESETYKECSMVCKEFLEDILSTIGVYLPQDDKSLVDLQQNHLSAVISGHVSPKLKELFHLLDSFRGDKQKQCLILVERIITAKVIERFVKKEASLAYLNVLYLTENNPSTNVSAQKMQIEIPDLFQHGKVNLLFITDVVEEGFQVPDCSCMVCFDLPKTMCSYSQSQKHAKQSNSKSIMFLERGNPKQRDHLHDLMRREVLIQDPEAPNLKSCPPPVKNGHGVKEIGSMVIPDSNITVSEEAASTQTMSDPPSRNEQLPPCKKLRLDNNLLQSNGKEKVASSKSKSSSSAAGSKKRKELHGTTCANALSGTWGENIDGATFQAYKFDFCCNISGEVYSSFSLLLESTLAEDVGKVEMDLYLVRKLVKASVSPCGQIRLSQEELVKAKYFQQFFFNGMFGKLFVGSKSQGTKREFLLQTDTSSLWHPAFMFLLLPVETNDLASSATIDWSAINSCASIVEFLKKNSLLDLRDSDGNQCNTSSGQEVLLDDKMEETNLIHFANASSDKNSLEELVVIAIHTGRIYSIVEAVSDSSAMSPFEVDASSGYATYAEYFNKKYGIVLAHPNQPLMKLKQSHHAHNLLVDFNEEMVVKTEPKAGNVRKRKPNIHAHLPPELLARIDVPRAVLKSIYLLPSVMHRLESLMLASQLREEIDCSIDNFSISSTSILEAVTTLTCPESFSMERLELLGDSVLKYVASCHLFLKYPDKDEGQLSRQRQSIISNSNLHRLTTSRKLQGYIRNGAFEPRRWTAPGQFSLFPVPCKCGIDTREVPLDPKFFTENMTIKIGKSCDMGHRWVVSKSVSDCAEALIGAYYVSGGLSASLHMMKWLGIDVDFDPNLVVEAINRVSLRCYIPKEDELIELERKIQHEFSAKFLLKEAITHSSLRESYSYERLEFLGDSVLDFLITRHLFNTYEQTGPGEMTDLRSACVNNENFAQVAVKNNLHTHLQRCATVLETQINDYLMSFQKPDETGRSIPSIQGPKALGDVVESIAGALLIDTRLDLDQVWRVFEPLLSPLVTPDKLQLPPYRELNELCDSLGYFFRVKCSNDGVKAQATIQLQLDDVLLTGDGSEQTNKLALGKAASHLLTQLEKRNISRKTSLGDNQSSMDVNLACNHSDRETLTSETTEIQSIVIPFIGPINMKKGGPRGTLHEFCKKHLWPMPTFDTSEEKSRWR

Target ORF information:

RefSeq Version	NM_001339115.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana dicer-like 3 (DCL3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001339115.1

ORF Insert Sequence:

ATGCATTCGT CGTTGGAGCC GGAGAAAATG GAGGAAGGTG GGGGAAGCAA TTCGCTTAAG 
AGAAAATTCT CTGAAATCGA TGGAGATCAA AATCTTGATT CTGTCTCTTC TCCTATGATG 
ACTGACTCTA ATGGTAGTTA TGAATTGAAA GTGTACGAGG TTGCTAAGAA CAGGAACATA 
ATTGCTGTTT TGGGGACAGG GATTGATAAG TCAGAGATCA CTAAGAGGCT TATCAAAGCT 
ATGGGTTCTT CTGATACAGA CAAAAGATTG ATAATTTTCT TGGCCCCAAC TGTGAATCTT 
GTTAAACAGC AATGCTGTGA GATCAGAGCA CTTGTGAATT TGAAAGTTGA AGAGTACTTT 
GGAGCTAAAG GAGTTGATAA ATGGACATCT CAGCGCTGGG ATGAGGAATT TAGCAAGCAC 
GATGTTTTAG TTATGACTCC TCAAATATTA TTGGATGTCC TTAGAAGTGC ATTCCTGAAA 
CTAGAGATGG TATGTCTTCT AATAATAGAT GAATGCCACC ATACCACTGG CAATCATCCC 
TATGCGAAGT TAATGAAGGA ATTCTATCAC GAATCCACTA GCAAACCGAA GATATTTGGA 
TTGACTGCGT CAGCCGTCAT TAGAAAAGCT CAAGTATCAG AACTTGAGAG ACTCATGGAC 
TCAAAGATTT TTAATCCTGA AGAGCGTGAA GGAGTGGAAA AGTTTGCTAC AACGGTTAAA 
GAAGGTCCAA TATTGTATAA CCCATCACCA TCCTGTAGTT TGGAATTGAA AGAAAAGTTA 
GAAACTTCAC ACCTCAAGTT TGATGCTTCT CTTAGAAGGC TTCAAGAGTT GGGAAAAGAC 
AGTTTTCTGA ATATGGATAA TAAGTTTGAG ACATATCAAA AGAGATTGTC TATCGACTAC 
AGAGAGATTT TGCATTGCCT TGATAATCTT GGCCTGATTT GCGCACACTT GGCGGCTGAA 
GTCTGCTTGG AGAAAATCTC AGATACGAAA GAGGAAAGTG AAACTTATAA AGAATGCTCA 
ATGGTGTGCA AGGAATTTCT TGAGGATATT TTATCCACCA TTGGGGTGTA TTTGCCGCAA 
GATGATAAGA GTCTGGTAGA TTTGCAGCAA AACCATCTGT CAGCAGTAAT TTCTGGGCAT 
GTATCTCCAA AGCTAAAAGA ACTCTTCCAT CTATTGGATT CCTTTAGAGG TGACAAGCAA 
AAGCAGTGCC TTATTTTAGT TGAGAGAATT ATAACTGCGA AAGTGATCGA AAGATTCGTT 
AAGAAAGAAG CCTCTTTGGC TTACCTTAAT GTCTTGTATT TAACCGAAAA CAACCCCTCC 
ACCAATGTAT CGGCACAGAA AATGCAAATT GAAATCCCTG ATTTATTTCA ACATGGCAAG 
GTGAATCTTT TATTCATCAC AGATGTGGTT GAAGAGGGAT TTCAGGTTCC AGATTGCTCA 
TGCATGGTTT GTTTTGACCT GCCCAAAACA ATGTGTAGTT ACTCGCAGTC TCAAAAACAT 
GCCAAACAGA GTAATTCTAA GTCTATCATG TTTCTTGAAA GAGGGAACCC GAAGCAAAGA 
GACCATCTGC ATGACCTTAT GCGAAGAGAA GTCCTAATTC AAGATCCAGA AGCTCCAAAC 
TTGAAATCGT GTCCACCTCC AGTGAAAAAT GGACACGGTG TGAAGGAGAT TGGATCCATG 
GTTATCCCAG ATTCTAACAT AACTGTATCT GAGGAAGCAG CTTCCACACA AACTATGAGT 
GATCCTCCTA GCAGAAATGA GCAGTTACCA CCGTGTAAAA AGTTACGCTT GGATAACAAT 
CTCTTACAAT CCAACGGCAA AGAGAAGGTT GCCTCTTCTA AAAGTAAATC ATCTTCATCG 
GCTGCAGGTT CAAAAAAACG TAAGGAGTTG CACGGAACAA CCTGTGCAAA CGCATTGTCA 
GGAACCTGGG GAGAAAATAT TGATGGCGCC ACCTTTCAGG CTTATAAGTT TGACTTCTGT 
TGTAATATTT CTGGCGAAGT ATACTCGAGT TTCTCTCTTT TGCTTGAGTC AACTCTCGCC 
GAGGATGTTG GTAAAGTTGA GATGGACCTT TACTTGGTCA GGAAGCTTGT CAAGGCTTCT 
GTCTCACCTT GTGGCCAGAT ACGTTTGAGT CAAGAGGAGC TGGTCAAAGC AAAATATTTT 
CAGCAGTTTT TCTTTAATGG CATGTTTGGA AAGTTGTTTG TTGGATCTAA GTCACAGGGA 
ACAAAGAGAG AATTTTTGCT TCAAACTGAC ACTAGTTCTC TTTGGCACCC TGCCTTTATG 
TTTCTACTGC TACCAGTTGA AACAAATGAT CTAGCTTCGA GTGCGACAAT TGATTGGTCA 
GCTATCAACT CCTGTGCCTC AATAGTTGAG TTCTTGAAGA AAAATTCTCT TCTTGATCTT 
CGGGATAGTG ATGGGAATCA GTGCAATACC TCATCCGGTC AGGAAGTCTT ACTAGACGAT 
AAAATGGAAG AAACGAATCT GATTCATTTT GCCAATGCTT CGTCTGATAA AAATAGTCTC 
GAAGAACTTG TGGTCATTGC AATTCATACT GGACGGATAT ACTCTATAGT TGAAGCCGTA 
AGCGATTCTT CTGCTATGAG CCCCTTTGAG GTGGATGCCT CATCAGGCTA TGCTACTTAT 
GCAGAATATT TTAACAAAAA GTATGGGATT GTTTTAGCGC ACCCGAACCA GCCGTTGATG 
AAGTTGAAGC AGAGTCACCA TGCGCACAAC CTTTTAGTCG ACTTCAATGA AGAGATGGTT 
GTGAAGACAG AACCAAAAGC TGGCAATGTT AGGAAAAGAA AACCGAATAT CCATGCGCAT 
TTGCCTCCAG AGCTTTTGGC TAGAATTGAT GTACCGCGTG CTGTGCTAAA ATCAATCTAC 
TTGCTGCCTT CAGTGATGCA CCGCCTAGAG TCTCTAATGT TGGCCAGCCA GCTTAGGGAA 
GAGATTGATT GTAGCATAGA TAACTTCAGT ATATCAAGTA CATCGATTCT TGAAGCAGTT 
ACAACACTTA CATGCCCCGA ATCATTTTCA ATGGAGCGGT TGGAACTGCT CGGGGATTCA 
GTCTTGAAGT ATGTTGCGAG CTGTCATCTA TTCCTTAAGT ATCCTGACAA AGATGAGGGG 
CAACTATCAC GGCAGAGACA ATCGATTATA TCTAACTCAA ATCTTCACCG CTTGACAACC 
AGTCGCAAAC TACAGGGATA CATAAGAAAT GGCGCTTTTG AACCGCGTCG CTGGACTGCA 
CCTGGTCAAT TTTCTCTTTT TCCTGTTCCT TGCAAGTGTG GGATTGATAC TAGAGAAGTA 
CCATTGGACC CAAAATTCTT CACAGAAAAC ATGACTATCA AAATAGGCAA GTCTTGCGAC 
ATGGGTCATA GATGGGTAGT TTCAAAATCT GTATCAGATT GCGCTGAGGC CCTGATTGGT 
GCCTATTATG TAAGCGGTGG ATTGTCTGCT TCTCTCCATA TGATGAAATG GCTCGGTATT 
GACGTCGATT TTGACCCAAA CCTAGTCGTT GAAGCCATCA ATAGAGTTTC TCTACGGTGT 
TACATTCCTA AAGAAGATGA GCTCATAGAG TTGGAGAGAA AGATCCAACA TGAATTCTCT 
GCAAAGTTTC TTTTAAAAGA GGCTATCACA CACTCCTCTC TTCGTGAATC CTATTCATAC 
GAGAGATTAG AGTTTCTTGG CGATTCTGTA CTGGATTTTC TAATAACCCG TCATCTTTTT 
AACACCTACG AACAAACTGG GCCTGGAGAG ATGACCGATC TTCGTTCTGC ATGTGTAAAC 
AATGAAAATT TTGCGCAAGT TGCAGTGAAA AATAACCTGC ATACCCACCT TCAACGCTGT 
GCTACGGTTC TCGAGACTCA AATAAACGAC TATCTGATGT CCTTTCAAAA GCCAGATGAG 
ACTGGTAGAT CAATCCCTTC AATACAGGGC CCTAAGGCTC TTGGAGATGT TGTGGAGAGT 
ATCGCTGGAG CATTGCTGAT CGATACGAGG TTAGATCTCG ATCAAGTGTG GAGAGTCTTT 
GAGCCGTTGC TTTCTCCACT TGTAACTCCA GATAAACTTC AGCTTCCTCC ATACCGGGAG 
CTCAATGAGC TATGCGACTC TCTTGGGTAT TTCTTTCGAG TGAAATGTTC AAATGATGGT 
GTCAAAGCAC AAGCCACGAT CCAGTTGCAG CTGGATGATG TTCTTTTAAC TGGAGATGGA 
TCTGAACAGA CAAATAAACT GGCCTTGGGA AAAGCAGCTT CACATCTGCT TACACAACTT 
GAGAAGAGAA ACATTTCACG TAAAACCTCG CTCGGGGATA ATCAAAGTTC CATGGATGTC 
AATCTTGCTT GCAATCATAG CGACAGAGAA ACTCTGACTT CAGAGACTAC TGAAATCCAG 
AGTATAGTGA TTCCATTTAT TGGACCTATA AACATGAAGA AAGGCGGGCC TCGTGGAACT 
CTACATGAGT TTTGCAAGAA GCATCTGTGG CCAATGCCTA CTTTCGATAC CTCGGAAGAG 
AAATCCAGAT GGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCL3 ( NM_001339114.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001325812.1 Arabidopsis thaliana dicer-like 3 (DCL3), partial mRNA.

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CloneID	OAb37990
Clone ID Related Accession (Same CDS sequence)	NM_001339114.1
Accession Version	NM_001339114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	dicer-like 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). COMPLETENESS: incomplete on the 3' end.

ATGCATTCGT CGTTGGAGCC GGAGAAAATG GAGGAAGGTG GGGGAAGCAA TTCGCTTAAG 
AGAAAATTCT CTGAAATCGA TGGAGATCAA AATCTTGATT CTGTCTCTTC TCCTATGATG 
ACTGACTCTA ATGGTTATGA ATTGAAAGTG TACGAGGTTG CTAAGAACAG GAACATAATT 
GCTGTTTTGG GGACAGGGAT TGATAAGTCA GAGATCACTA AGAGGCTTAT CAAAGCTATG 
GGTTCTTCTG ATACAGACAA AAGATTGATA ATTTTCTTGG CCCCAACTGT GAATCTTGTT 
AAACAGCAAT GCTGTGAGAT CAGAGCACTT GTGAATTTGA AAGTTGAAGA GTACTTTGGA 
GCTAAAGGAG TTGATAAATG GACATCTCAG CGCTGGGATG AGGAATTTAG CAAGCACGAT 
GTTTTAGTTA TGACTCCTCA AATATTATTG GATGTCCTTA GAAGTGCATT CCTGAAACTA 
GAGATGGTAT GTCTTCTAAT AATAGATGAA TGCCACCATA CCACTGGCAA TCATCCCTAT 
GCGAAGTTAA TGAAGGAATT CTATCACGAA TCCACTAGCA AACCGAAGAT ATTTGGATTG 
ACTGCGTCAG CCGTCATTAG AAAAGCTCAA GTATCAGAAC TTGAGAGACT CATGGACTCA 
AAGATTTTTA ATCCTGAAGA GCGTGAAGGA GTGGAAAAGT TTGCTACAAC GGTTAAAGAA 
GGTCCAATAT TGTATAACCC ATCACCATCC TGTAGTTTGG AATTGAAAGA AAAGTTAGAA 
ACTTCACACC TCAAGTTTGA TGCTTCTCTT AGAAGGCTTC AAGAGTTGGG AAAAGACAGT 
TTTCTGAATA TGGATAATAA GTTTGAGACA TATCAAAAGA GATTGTCTAT CGACTACAGA 
GAGATTTTGC ATTGCCTTGA TAATCTTGGC CTGATTTGCG CACACTTGGC GGCTGAAGTC 
TGCTTGGAGA AAATCTCAGA TACGAAAGAG GAAAGTGAAA CTTATAAAGA ATGCTCAATG 
GTGTGCAAGG AATTTCTTGA GGATATTTTA TCCACCATTG GGGTGTATTT GCCGCAAGAT 
GATAAGAGTC TGGTAGATTT GCAGCAAAAC CATCTGTCAG CAGTAATTTC TGGGCATGTA 
TCTCCAAAGC TAAAAGAACT CTTCCATCTA TTGGATTCCT TTAGAGGTGA CAAGCAAAAG 
CAGTGCCTTA TTTTAGTTGA GAGAATTATA ACTGCGAAAG TGATCGAAAG ATTCGTTAAG 
AAAGAAGCCT CTTTGGCTTA CCTTAATGTC TTGTATTTAA CCGAAAACAA CCCCTCCACC 
AATGTATCGG CACAGAAAAT GCAAATTGAA ATCCCTGATT TATTTCAACA TGGCAAGGTG 
AATCTTTTAT TCATCACAGA TGTGGTTGAA GAGGGATTTC AGGTTCCAGA TTGCTCATGC 
ATGGTTTGTT TTGACCTGCC CAAAACAATG TGTAGTTACT CGCAGTCTCA AAAACATGCC 
AAACAGAGTA ATTCTAAGTC TATCATGTTT CTTGAAAGAG GGAACCCGAA GCAAAGAGAC 
CATCTGCATG ACCTTATGCG AAGAGAAGTC CTAATTCAAG ATCCAGAAGC TCCAAACTTG 
AAATCGTGTC CACCTCCAGT GAAAAATGGA CACGGTGTGA AGGAGATTGG ATCCATGGTT 
ATCCCAGATT CTAACATAAC TGTATCTGAG GAAGCAGCTT CCACACAAAC TATGAGTGAT 
CCTCCTAGCA GAAATGAGCA GTTACCACCG TGTAAAAAGT TACGCTTGGA TAACAATCTC 
TTACAATCCA ACGGCAAAGA GAAGGTTGCC TCTTCTAAAA GTAAATCATC TTCATCGGCT 
GCAGGTTCAA AAAAACGTAA GGAGTTGCAC GGAACAACCT GTGCAAACGC ATTGTCAGGA 
ACCTGGGGAG AAAATATTGA TGGCGCCACC TTTCAGGCTT ATAAGTTTGA CTTCTGTTGT 
AATATTTCTG GCGAAGTATA CTCGAGTTTC TCTCTTTTGC TTGAGTCAAC TCTCGCCGAG 
GATGTTGGTA AAGTTGAGAT GGACCTTTAC TTGGTCAGGA AGCTTGTCAA GGCTTCTGTC 
TCACCTTGTG GCCAGATACG TTTGAGTCAA GAGGAGCTGG TCAAAGCAAA ATATTTTCAG 
CAGTTTTTCT TTAATGGCAT GTTTGGAAAG TTGTTTGTTG GATCTAAGTC ACAGGGAACA 
AAGAGAGAAT TTTTGCTTCA AACTGACACT AGTTCTCTTT GGCACCCTGC CTTTATGTTT 
CTACTGCTAC CAGTTGAAAC AAATGATCTA GCTTCGAGTG CGACAATTGA TTGGTCAGCT 
ATCAACTCCT GTGCCTCAAT AGTTGAGTTC TTGAAGAAAA ATTCTCTTCT TGATCTTCGG 
GATAGTGATG GGAATCAGTG CAATACCTCA TCCGGTCAGG AAGTCTTACT AGACGATAAA 
ATGGAAGAAA CGAATCTGAT TCATTTTGCC AATGCTTCGT CTGATAAAAA TAGTCTCGAA 
GAACTTGTGG TCATTGCAAT TCATACTGGA CGGATATACT CTATAGTTGA AGCCGTAAGC 
GATTCTTCTG CTATGAGCCC CTTTGAGGTG GATGCCTCAT CAGGCTATGC TACTTATGCA 
GAATATTTTA ACAAAAAGTA TGGGATTGTT TTAGCGCACC CGAACCAGCC GTTGATGAAG 
TTGAAGCAGA GTCACCATGC GCACAACCTT TTAGTCGACT TCAATGAAGA GATGGTTGTG 
AAGACAGAAC CAAAAGCTGG CAATGTTAGG AAAAGAAAAC CGAATATCCA TGCGCATTTG 
CCTCCAGAGC TTTTGGCTAG AATTGATGTA CCGCGTGCTG TGCTAAAATC AATCTACTTG 
CTGCCTTCAG TGATGCACCG CCTAGAGTCT CTAATGTTGG CCAGCCAGCT TAGGGAAGAG 
ATTGATTGTA GCATAGATAA CTTCAGTATA TCAAGTACAT CGATTCTTGA AGCAGTTACA 
ACACTTACAT GCCCCGAATC ATTTTCAATG GAGCGGTTGG AACTGCTCGG GGATTCAGTC 
TTGAAGTATG TTGCGAGCTG TCATCTATTC CTTAAGTATC CTGACAAAGA TGAGGGGCAA 
CTATCACGGC AGAGACAATC GATTATATCT AACTCAAATC TTCACCGCTT GACAACCAGT 
CGCAAACTAC AGGGATACAT AAGAAATGGC GCTTTTGAAC CGCGTCGCTG GACTGCACCT 
GGTCAATTTT CTCTTTTTCC TGTTCCTTGC AAGTGTGGGA TTGATACTAG AGAAGTACCA 
TTGGACCCAA AATTCTTCAC AGAAAACATG ACTATCAAAA TAGGCAAGTC TTGCGACATG 
GGTCATAGAT GGGTAGTTTC AAAATCTGTA TCAGATTGCG CTGAGGCCCT GATTGGTGCC 
TATTATGTAA GCGGTGGATT GTCTGCTTCT CTCCATATGA TGAAATGGCT CGGTATTGAC 
GTCGATTTTG ACCCAAACCT AGTCGTTGAA GCCATCAATA GAGTTTCTCT ACGGTGTTAC 
ATTCCTAAAG AAGATGAGCT CATAGAGTTG GAGAGAAAGA TCCAACATGA ATTCTCTGCA 
AAGTTTCTTT TAAAAGAGGC TATCACACAC TCCTCTCTTC GTGAATCCTA TTCATACGAG 
AGATTAGAGT TTCTTGGCGA TTCTGTACTG GATTTTCTAA TAACCCGTCA TCTTTTTAAC 
ACCTACGAAC AAACTGGGCC TGGAGAGATG ACCGATCTTC GTTCTGCATG TGTAAACAAT 
GAAAATTTTG CGCAAGTTGC AGTGAAAAAT AACCTGCATA CCCACCTTCA ACGCTGTGCT 
ACGGTTCTCG AGACTCAAAT AAACGACTAT CTGATGTCCT TTCAAAAGCC AGATGAGACT 
GGTAGATCAA TCCCTTCAAT ACAGGGCCCT AAGGCTCTTG GAGATGTTGT GGAGAGTATC 
GCTGGAGCAT TGCTGATCGA TACGAGGTTA GATCTCGATC AAGTGTGGAG AGTCTTTGAG 
CCGTTGCTTT CTCCACTTGT AACTCCAGAT AAACTTCAGC TTCCTCCATA CCGGGAGCTC 
AATGAGCTAT GCGACTCTCT TGGGTATTTC TTTCGAGTGA AATGTTCAAA TGATGGTGTC 
AAAGCACAAG CCACGATCCA GTTGCAGCTG GATGATGTTC TTTTAACTGG AGATGGATCT 
GAACAGACAA ATAAACTGGC CTTGGGAAAA GCAGCTTCAC ATCTGCTTAC ACAACTTGAG 
AAGAGAAACA TTTCACGTAA AACCTCGCTC GGGGATAATC AAAGTTCCAT GGATGTCAAT 
CTTGCTTGCA ATCATAGCGA CAGAGAAACT CTGACTTCAG AGACTACTGA AATCCAGAGT 
ATAGTGATTC CATTTATTGG ACCTATAAAC ATGAAGAAAG GCGGGCCTCG TGGAACTCTA 
CATGAGTTTT GCAAGAAGCA TCTGTGGCCA ATGCCTACTT TCGATACCTC GGAAGAGAAA 
TCCAGAACTC CGTTTGAATT CATAGATGGC GGTGAGAAGC GGACTAGCTT CAGCAGTTTC 
ACATCGACCA TAACCCTAAG GATACCCAAT CGTGAGGCTG TGATGTATGC TGGAGAAGCA 
AGGCCTGACA AGAAGAGTTC CTTCGACTCT GCAGTCGTGG AATTGCTTTA TGAGCTCGAG 
CGCCGCAAGA TCGTCATAAT ACAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001325812.1
CDS	162..4871
Translation	MHSSLEPEKMEEGGGSNSLKRKFSEIDGDQNLDSVSSPMMTDSNGYELKVYEVAKNRNIIAVLGTGIDKSEITKRLIKAMGSSDTDKRLIIFLAPTVNLVKQQCCEIRALVNLKVEEYFGAKGVDKWTSQRWDEEFSKHDVLVMTPQILLDVLRSAFLKLEMVCLLIIDECHHTTGNHPYAKLMKEFYHESTSKPKIFGLTASAVIRKAQVSELERLMDSKIFNPEEREGVEKFATTVKEGPILYNPSPSCSLELKEKLETSHLKFDASLRRLQELGKDSFLNMDNKFETYQKRLSIDYREILHCLDNLGLICAHLAAEVCLEKISDTKEESETYKECSMVCKEFLEDILSTIGVYLPQDDKSLVDLQQNHLSAVISGHVSPKLKELFHLLDSFRGDKQKQCLILVERIITAKVIERFVKKEASLAYLNVLYLTENNPSTNVSAQKMQIEIPDLFQHGKVNLLFITDVVEEGFQVPDCSCMVCFDLPKTMCSYSQSQKHAKQSNSKSIMFLERGNPKQRDHLHDLMRREVLIQDPEAPNLKSCPPPVKNGHGVKEIGSMVIPDSNITVSEEAASTQTMSDPPSRNEQLPPCKKLRLDNNLLQSNGKEKVASSKSKSSSSAAGSKKRKELHGTTCANALSGTWGENIDGATFQAYKFDFCCNISGEVYSSFSLLLESTLAEDVGKVEMDLYLVRKLVKASVSPCGQIRLSQEELVKAKYFQQFFFNGMFGKLFVGSKSQGTKREFLLQTDTSSLWHPAFMFLLLPVETNDLASSATIDWSAINSCASIVEFLKKNSLLDLRDSDGNQCNTSSGQEVLLDDKMEETNLIHFANASSDKNSLEELVVIAIHTGRIYSIVEAVSDSSAMSPFEVDASSGYATYAEYFNKKYGIVLAHPNQPLMKLKQSHHAHNLLVDFNEEMVVKTEPKAGNVRKRKPNIHAHLPPELLARIDVPRAVLKSIYLLPSVMHRLESLMLASQLREEIDCSIDNFSISSTSILEAVTTLTCPESFSMERLELLGDSVLKYVASCHLFLKYPDKDEGQLSRQRQSIISNSNLHRLTTSRKLQGYIRNGAFEPRRWTAPGQFSLFPVPCKCGIDTREVPLDPKFFTENMTIKIGKSCDMGHRWVVSKSVSDCAEALIGAYYVSGGLSASLHMMKWLGIDVDFDPNLVVEAINRVSLRCYIPKEDELIELERKIQHEFSAKFLLKEAITHSSLRESYSYERLEFLGDSVLDFLITRHLFNTYEQTGPGEMTDLRSACVNNENFAQVAVKNNLHTHLQRCATVLETQINDYLMSFQKPDETGRSIPSIQGPKALGDVVESIAGALLIDTRLDLDQVWRVFEPLLSPLVTPDKLQLPPYRELNELCDSLGYFFRVKCSNDGVKAQATIQLQLDDVLLTGDGSEQTNKLALGKAASHLLTQLEKRNISRKTSLGDNQSSMDVNLACNHSDRETLTSETTEIQSIVIPFIGPINMKKGGPRGTLHEFCKKHLWPMPTFDTSEEKSRTPFEFIDGGEKRTSFSSFTSTITLRIPNREAVMYAGEARPDKKSSFDSAVVELLYELERRKIVIIQK

Target ORF information:

RefSeq Version	NM_001339114.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana dicer-like 3 (DCL3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001339114.1

ORF Insert Sequence:

ATGCATTCGT CGTTGGAGCC GGAGAAAATG GAGGAAGGTG GGGGAAGCAA TTCGCTTAAG 
AGAAAATTCT CTGAAATCGA TGGAGATCAA AATCTTGATT CTGTCTCTTC TCCTATGATG 
ACTGACTCTA ATGGTTATGA ATTGAAAGTG TACGAGGTTG CTAAGAACAG GAACATAATT 
GCTGTTTTGG GGACAGGGAT TGATAAGTCA GAGATCACTA AGAGGCTTAT CAAAGCTATG 
GGTTCTTCTG ATACAGACAA AAGATTGATA ATTTTCTTGG CCCCAACTGT GAATCTTGTT 
AAACAGCAAT GCTGTGAGAT CAGAGCACTT GTGAATTTGA AAGTTGAAGA GTACTTTGGA 
GCTAAAGGAG TTGATAAATG GACATCTCAG CGCTGGGATG AGGAATTTAG CAAGCACGAT 
GTTTTAGTTA TGACTCCTCA AATATTATTG GATGTCCTTA GAAGTGCATT CCTGAAACTA 
GAGATGGTAT GTCTTCTAAT AATAGATGAA TGCCACCATA CCACTGGCAA TCATCCCTAT 
GCGAAGTTAA TGAAGGAATT CTATCACGAA TCCACTAGCA AACCGAAGAT ATTTGGATTG 
ACTGCGTCAG CCGTCATTAG AAAAGCTCAA GTATCAGAAC TTGAGAGACT CATGGACTCA 
AAGATTTTTA ATCCTGAAGA GCGTGAAGGA GTGGAAAAGT TTGCTACAAC GGTTAAAGAA 
GGTCCAATAT TGTATAACCC ATCACCATCC TGTAGTTTGG AATTGAAAGA AAAGTTAGAA 
ACTTCACACC TCAAGTTTGA TGCTTCTCTT AGAAGGCTTC AAGAGTTGGG AAAAGACAGT 
TTTCTGAATA TGGATAATAA GTTTGAGACA TATCAAAAGA GATTGTCTAT CGACTACAGA 
GAGATTTTGC ATTGCCTTGA TAATCTTGGC CTGATTTGCG CACACTTGGC GGCTGAAGTC 
TGCTTGGAGA AAATCTCAGA TACGAAAGAG GAAAGTGAAA CTTATAAAGA ATGCTCAATG 
GTGTGCAAGG AATTTCTTGA GGATATTTTA TCCACCATTG GGGTGTATTT GCCGCAAGAT 
GATAAGAGTC TGGTAGATTT GCAGCAAAAC CATCTGTCAG CAGTAATTTC TGGGCATGTA 
TCTCCAAAGC TAAAAGAACT CTTCCATCTA TTGGATTCCT TTAGAGGTGA CAAGCAAAAG 
CAGTGCCTTA TTTTAGTTGA GAGAATTATA ACTGCGAAAG TGATCGAAAG ATTCGTTAAG 
AAAGAAGCCT CTTTGGCTTA CCTTAATGTC TTGTATTTAA CCGAAAACAA CCCCTCCACC 
AATGTATCGG CACAGAAAAT GCAAATTGAA ATCCCTGATT TATTTCAACA TGGCAAGGTG 
AATCTTTTAT TCATCACAGA TGTGGTTGAA GAGGGATTTC AGGTTCCAGA TTGCTCATGC 
ATGGTTTGTT TTGACCTGCC CAAAACAATG TGTAGTTACT CGCAGTCTCA AAAACATGCC 
AAACAGAGTA ATTCTAAGTC TATCATGTTT CTTGAAAGAG GGAACCCGAA GCAAAGAGAC 
CATCTGCATG ACCTTATGCG AAGAGAAGTC CTAATTCAAG ATCCAGAAGC TCCAAACTTG 
AAATCGTGTC CACCTCCAGT GAAAAATGGA CACGGTGTGA AGGAGATTGG ATCCATGGTT 
ATCCCAGATT CTAACATAAC TGTATCTGAG GAAGCAGCTT CCACACAAAC TATGAGTGAT 
CCTCCTAGCA GAAATGAGCA GTTACCACCG TGTAAAAAGT TACGCTTGGA TAACAATCTC 
TTACAATCCA ACGGCAAAGA GAAGGTTGCC TCTTCTAAAA GTAAATCATC TTCATCGGCT 
GCAGGTTCAA AAAAACGTAA GGAGTTGCAC GGAACAACCT GTGCAAACGC ATTGTCAGGA 
ACCTGGGGAG AAAATATTGA TGGCGCCACC TTTCAGGCTT ATAAGTTTGA CTTCTGTTGT 
AATATTTCTG GCGAAGTATA CTCGAGTTTC TCTCTTTTGC TTGAGTCAAC TCTCGCCGAG 
GATGTTGGTA AAGTTGAGAT GGACCTTTAC TTGGTCAGGA AGCTTGTCAA GGCTTCTGTC 
TCACCTTGTG GCCAGATACG TTTGAGTCAA GAGGAGCTGG TCAAAGCAAA ATATTTTCAG 
CAGTTTTTCT TTAATGGCAT GTTTGGAAAG TTGTTTGTTG GATCTAAGTC ACAGGGAACA 
AAGAGAGAAT TTTTGCTTCA AACTGACACT AGTTCTCTTT GGCACCCTGC CTTTATGTTT 
CTACTGCTAC CAGTTGAAAC AAATGATCTA GCTTCGAGTG CGACAATTGA TTGGTCAGCT 
ATCAACTCCT GTGCCTCAAT AGTTGAGTTC TTGAAGAAAA ATTCTCTTCT TGATCTTCGG 
GATAGTGATG GGAATCAGTG CAATACCTCA TCCGGTCAGG AAGTCTTACT AGACGATAAA 
ATGGAAGAAA CGAATCTGAT TCATTTTGCC AATGCTTCGT CTGATAAAAA TAGTCTCGAA 
GAACTTGTGG TCATTGCAAT TCATACTGGA CGGATATACT CTATAGTTGA AGCCGTAAGC 
GATTCTTCTG CTATGAGCCC CTTTGAGGTG GATGCCTCAT CAGGCTATGC TACTTATGCA 
GAATATTTTA ACAAAAAGTA TGGGATTGTT TTAGCGCACC CGAACCAGCC GTTGATGAAG 
TTGAAGCAGA GTCACCATGC GCACAACCTT TTAGTCGACT TCAATGAAGA GATGGTTGTG 
AAGACAGAAC CAAAAGCTGG CAATGTTAGG AAAAGAAAAC CGAATATCCA TGCGCATTTG 
CCTCCAGAGC TTTTGGCTAG AATTGATGTA CCGCGTGCTG TGCTAAAATC AATCTACTTG 
CTGCCTTCAG TGATGCACCG CCTAGAGTCT CTAATGTTGG CCAGCCAGCT TAGGGAAGAG 
ATTGATTGTA GCATAGATAA CTTCAGTATA TCAAGTACAT CGATTCTTGA AGCAGTTACA 
ACACTTACAT GCCCCGAATC ATTTTCAATG GAGCGGTTGG AACTGCTCGG GGATTCAGTC 
TTGAAGTATG TTGCGAGCTG TCATCTATTC CTTAAGTATC CTGACAAAGA TGAGGGGCAA 
CTATCACGGC AGAGACAATC GATTATATCT AACTCAAATC TTCACCGCTT GACAACCAGT 
CGCAAACTAC AGGGATACAT AAGAAATGGC GCTTTTGAAC CGCGTCGCTG GACTGCACCT 
GGTCAATTTT CTCTTTTTCC TGTTCCTTGC AAGTGTGGGA TTGATACTAG AGAAGTACCA 
TTGGACCCAA AATTCTTCAC AGAAAACATG ACTATCAAAA TAGGCAAGTC TTGCGACATG 
GGTCATAGAT GGGTAGTTTC AAAATCTGTA TCAGATTGCG CTGAGGCCCT GATTGGTGCC 
TATTATGTAA GCGGTGGATT GTCTGCTTCT CTCCATATGA TGAAATGGCT CGGTATTGAC 
GTCGATTTTG ACCCAAACCT AGTCGTTGAA GCCATCAATA GAGTTTCTCT ACGGTGTTAC 
ATTCCTAAAG AAGATGAGCT CATAGAGTTG GAGAGAAAGA TCCAACATGA ATTCTCTGCA 
AAGTTTCTTT TAAAAGAGGC TATCACACAC TCCTCTCTTC GTGAATCCTA TTCATACGAG 
AGATTAGAGT TTCTTGGCGA TTCTGTACTG GATTTTCTAA TAACCCGTCA TCTTTTTAAC 
ACCTACGAAC AAACTGGGCC TGGAGAGATG ACCGATCTTC GTTCTGCATG TGTAAACAAT 
GAAAATTTTG CGCAAGTTGC AGTGAAAAAT AACCTGCATA CCCACCTTCA ACGCTGTGCT 
ACGGTTCTCG AGACTCAAAT AAACGACTAT CTGATGTCCT TTCAAAAGCC AGATGAGACT 
GGTAGATCAA TCCCTTCAAT ACAGGGCCCT AAGGCTCTTG GAGATGTTGT GGAGAGTATC 
GCTGGAGCAT TGCTGATCGA TACGAGGTTA GATCTCGATC AAGTGTGGAG AGTCTTTGAG 
CCGTTGCTTT CTCCACTTGT AACTCCAGAT AAACTTCAGC TTCCTCCATA CCGGGAGCTC 
AATGAGCTAT GCGACTCTCT TGGGTATTTC TTTCGAGTGA AATGTTCAAA TGATGGTGTC 
AAAGCACAAG CCACGATCCA GTTGCAGCTG GATGATGTTC TTTTAACTGG AGATGGATCT 
GAACAGACAA ATAAACTGGC CTTGGGAAAA GCAGCTTCAC ATCTGCTTAC ACAACTTGAG 
AAGAGAAACA TTTCACGTAA AACCTCGCTC GGGGATAATC AAAGTTCCAT GGATGTCAAT 
CTTGCTTGCA ATCATAGCGA CAGAGAAACT CTGACTTCAG AGACTACTGA AATCCAGAGT 
ATAGTGATTC CATTTATTGG ACCTATAAAC ATGAAGAAAG GCGGGCCTCG TGGAACTCTA 
CATGAGTTTT GCAAGAAGCA TCTGTGGCCA ATGCCTACTT TCGATACCTC GGAAGAGAAA 
TCCAGAACTC CGTTTGAATT CATAGATGGC GGTGAGAAGC GGACTAGCTT CAGCAGTTTC 
ACATCGACCA TAACCCTAAG GATACCCAAT CGTGAGGCTG TGATGTATGC TGGAGAAGCA 
AGGCCTGACA AGAAGAGTTC CTTCGACTCT GCAGTCGTGG AATTGCTTTA TGAGCTCGAG 
CGCCGCAAGA TCGTCATAAT ACAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCL3 ( NM_114260.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_189978.1 Arabidopsis thaliana dicer-like 3 (DCL3), partial mRNA.

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CloneID	OAb12769
Clone ID Related Accession (Same CDS sequence)	NM_114260.1
Accession Version	NM_114260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	dicer-like 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). COMPLETENESS: incomplete on both ends.

ATGCATTCGT CGTTGGAGCC GGAGAAAATG GAGGAAGGTG GGGGAAGCAA TTCGCTTAAG 
AGAAAATTCT CTGAAATCGA TGGAGATCAA AATCTTGATT CTGTCTCTTC TCCTATGATG 
ACTGACTCTA ATGGTAGTTA TGAATTGAAA GTGTACGAGG TTGCTAAGAA CAGGAACATA 
ATTGCTGTTT TGGGGACAGG GATTGATAAG TCAGAGATCA CTAAGAGGCT TATCAAAGCT 
ATGGGTTCTT CTGATACAGA CAAAAGATTG ATAATTTTCT TGGCCCCAAC TGTGAATCTT 
CAATGCTGTG AGATCAGAGC ACTTGTGAAT TTGAAAGTTG AAGAGTACTT TGGAGCTAAA 
GGAGTTGATA AATGGACATC TCAGCGCTGG GATGAGGAAT TTAGCAAGCA CGATGTTTTA 
GTTATGACTC CTCAAATATT ATTGGATGTC CTTAGAAGTG CATTCCTGAA ACTAGAGATG 
GTATGTCTTC TAATAATAGA TGAATGCCAC CATACCACTG GCAATCATCC CTATGCGAAG 
TTAATGAAGA TTTTTAATCC TGAAGAGCGT GAAGGAGTGG AAAAGTTTGC TACAACGGTT 
AAAGAAGGTC CAATATTGTA TAACCCATCA CCATCCTGTA GTTTGGAATT GAAAGAAAAG 
TTAGAAACTT CACACCTCAA GTTTGATGCT TCTCTTAGAA GGCTTCAAGA GTTGGGAAAA 
GACAGTTTTC TGAATATGGA TAATAAGTTT GAGACATATC AAAAGAGATT GTCTATCGAC 
TACAGAGAGA TTTTGCATTG CCTTGATAAT CTTGGCCTGA TTTGCGCACA CTTGGCGGCT 
GAAGTCTGCT TGGAGAAAAT CTCAGATACG AAAGAGGAAA GTGAAACTTA TAAAGAATGC 
TCAATGGTGT GCAAGGAATT TCTTGAGGAT ATTTTATCCA CCATTGGGGT GTATTTGCCG 
CAAGATGATA AGAGTCTGGT AGATTTGCAG CAAAACCATC TGTCAGCAGT AATTTCTGGG 
CATGTATCTC CAAAGCTAAA AGAACTCTTC CATCTATTGG ATTCCTTTAG AGGTGACAAG 
CAAAAGCAGT GCCTTATTTT AGTTGAGAGA ATTATAACTG CGAAAGTGAT CGAAAGATTC 
GTTAAGAAAG AAGCCTCTTT GGCTTACCTT AATGTCTTGT ATTTAACCGA AAACAACCCC 
TCCACCAATG TATCGGCACA GAAAATGCAA ATTGAAATCC CTGATTTATT TCAACATGGC 
AAGGTGAATC TTTTATTCAT CACAGATGTG GTTGAAGAGG GATTTCAGGT TCCAGATTGC 
TCATGCATGG TTTGTTTTGA CCTGCCCAAA ACAATGTGTA GTTACTCGCA GTCTCAAAAA 
CATGCCAAAC AGAGTAATTC TAAGTCTATC ATGTTTCTTG AAAGAGGGAA CCCGAAGCAA 
AGAGACCATC TGCATGACCT TATGCGAAGA GAAGTCCTAA TTCAAGATCC AGAAGCTCCA 
AACTTGAAAT CGTGTCCACC TCCAGTGAAA AATGGACACG GTGTGAAGGA GATTGGATCC 
ATGGTTATCC CAGATTCTAA CATAACTGTA TCTGAGGAAG CAGCTTCCAC ACAAACTATG 
AGTGATCCTC CTAGCAGAAA TGAGCAGTTA CCACCGTGTA AAAAGTTACG CTTGGATAAC 
AATCTCTTAC AATCCAACGG CAAAGAGAAG GTTGCCTCTT CTAAAAGTAA ATCATCTTCA 
TCGGCTGCAG GTTCAAAAAA ACGTAAGGAG TTGCACGGAA CAACCTGTGC AAACGCATTG 
TCAGGAACCT GGGGAGAAAA TATTGATGGC GCCACCTTTC AGGCTTATAA GTTTGACTTC 
TGTTGTAATA TTTCTGGCGA AGTATACTCG AGTTTCTCTC TTTTGCTTGA GTCAACTCTC 
GCCGAGGATG TTGGTAAAGT TGAGATGGAC CTTTACTTGG TCAGGAAGCT TGTCAAGGCT 
TCTGTCTCAC CTTGTGGCCA GATACGTTTG AGTCAAGAGG AGCTGGTCAA AGCAAAATAT 
TTTCAGCAGT TTTTCTTTAA TGGCATGTTT GGAAAGTTGT TTGTTGGATC TAAGTCACAG 
GGAACAAAGA GAGAATTTTT GCTTCAAACT GACACTAGTT CTCTTTGGCA CCCTGCCTTT 
ATGTTTCTAC TGCTACCAGT TGAAACAAAT GATCTAGCTT CGAGTGCGAC AATTGATTGG 
TCAGCTATCA ACTCCTGTGC CTCAATAGTT GAGTTCTTGA AGAAAAATTC TCTTCTTGAT 
CTTCGGGATA GTGATGGGAA TCAGTGCAAT ACCTCATCCG GTCAGGAAGT CTTACTAGAC 
GATAAAATGG AAGAAACGAA TCTGATTCAT TTTGCCAATG CTTCGTCTGA TAAAAATAGT 
CTCGAAGAAC TTGTGGTCAT TGCAATTCAT ACTGGACGGA TATACTCTAT AGTTGAAGCC 
GTAAGCGATT CTTCTGCTAT GAGCCCCTTT GAGGTGGATG CCTCATCAGG CTATGCTACT 
TATGCAGAAT ATTTTAACAA AAAGTATGGG ATTGTTTTAG CGCACCCGAA CCAGCCGTTG 
ATGAAGTTGA AGCAGAGTCA CCATGCGCAC AACCTTTTAG TCGACTTCAA TGAAGAGATG 
GTTGTGAAGA CAGAACCAAA AGCTGGCAAT GTTAGGAAAA GAAAACCGAA TATCCATGCG 
CATTTGCCTC CAGAGCTTTT GGCTAGAATT GATGTACCGC GTGCTGTGCT AAAATCAATC 
TACTTGCTGC CTTCAGTGAT GCACCGCCTA GAGTCTCTAA TGTTGGCCAG CCAGCTTAGG 
GAAGAGATTG ATTGTAGCAT AGATAACTTC AGTATATCAA GTACATCGAT TCTTGAAGCA 
GTTACAACAC TTACATGCCC CGAATCATTT TCAATGGAGC GGTTGGAACT GCTCGGGGAT 
TCAGTCTTGA AGTATGTTGC GAGCTGTCAT CTATTCCTTA AGTATCCTGA CAAAGATGAG 
GGGCAACTAT CACGGCAGAG ACAATCGATT ATATCTAACT CAAATCTTCA CCGCTTGACA 
ACCAGTCGCA AACTACAGGG ATACATAAGA AATGGCGCTT TTGAACCGCG TCGCTGGACT 
GCACCTGGTC AATTTTCTCT TTTTCCTGTT CCTTGCAAGT GTGGGATTGA TACTAGAGAA 
GTACCATTGG ACCCAAAATT CTTCACAGAA AACATGACTA TCAAAATAGG CAAGTCTTGC 
GACATGGGTC ATAGATGGGT AGTTTCAAAA TCTGTATCAG ATTGCGCTGA GGCCCTGATT 
GGTGCCTATT ATGTAAGCGG TGGATTGTCT GCTTCTCTCC ATATGATGAA ATGGCTCGGT 
ATTGACGTCG ATTTTGACCC AAACCTAGTC GTTGAAGCCA TCAATAGAGT TTCTCTACGG 
TGTTACATTC CTAAAGAAGA TGAGCTCATA GAGTTGGAGA GAAAGATCCA ACATGAATTC 
TCTGCAAAGT TTCTTTTAAA AGAGGCTATC ACACACTCCT CTCTTCGTGA ATCCTATTCA 
TACGAGAGAT TAGAGTTTCT TGGCGATTCT GTACTGGATT TTCTAATAAC CCGTCATCTT 
TTTAACACCT ACGAACAAAC TGGGCCTGGA GAGATGACCG ATCTTCGTTC TGCATGTGTA 
AACAATGAAA ATTTTGCGCA AGTTGCAGTG AAAAATAACC TGCATACCCA CCTTCAACGC 
TGTGCTACGG TTCTCGAGAC TCAAATAAAC GACTATCTGA TGTCCTTTCA AAAGCCAGAT 
GAGACTGGTA GATCAATCCC TTCAATACAG GGCCCTAAGG CTCTTGGAGA TGTTGTGGAG 
AGTATCGCTG GAGCATTGCT GATCGATACG AGGTTAGATC TCGATCAAGT GTGGAGAGTC 
TTTGAGCCGT TGCTTTCTCC ACTTGTAACT CCAGATAAAC TTCAGCTTCC TCCATACCGG 
GAGCTCAATG AGCTATGCGA CTCTCTTGGG TATTTCTTTC GAGTGAAATG TTCAAATGAT 
GGTGTCAAAG CACAAGCCAC GATCCAGTTG CAGCTGGATG ATGTTCTTTT AACTGGAGAT 
GGATCTGAAC AGACAAATAA ACTGGCCTTG GGAAAAGCAG CTTCACATCT GCTTACACAA 
CTTGAGAAGA GAAACATTTC ACGTAAAACC TCGCTCGGGG ATAATCAAAG TTCCATGGAT 
GTCAATCTTG CTTGCAATCA TAGCGACAGA GAAACTCTGA CTTCAGAGAC TACTGAAATC 
CAGAGTATAG TGATTCCATT TATTGGACCT ATAAACATGA AGAAAGGCGG GCCTCGTGGA 
ACTCTACATG AGTTTTGCAA GAAGCATCTG TGGCCAATGC CTACTTTCGA TACCTCGGAA 
GAGAAATCCA GAACTCCGTT TGAATTCATA GATGGCGGTG AGAAGCGGAC TAGCTTCAGC 
AGTTTCACAT CGACCATAAC CCTAAGGATA CCCAATCGTG AGGCTGTGAT GTATGCTGGA 
GAAGCAAGGC CTGACAAGAA GAGTTCCTTC GACTCTGCAG TCGTGGAATT GCTTTATGAG 
CTCGAGCGCC GCAAGATCGT CATAATACAA AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_189978.1
CDS	1..4596
Translation	MHSSLEPEKMEEGGGSNSLKRKFSEIDGDQNLDSVSSPMMTDSNGSYELKVYEVAKNRNIIAVLGTGIDKSEITKRLIKAMGSSDTDKRLIIFLAPTVNLQCCEIRALVNLKVEEYFGAKGVDKWTSQRWDEEFSKHDVLVMTPQILLDVLRSAFLKLEMVCLLIIDECHHTTGNHPYAKLMKIFNPEEREGVEKFATTVKEGPILYNPSPSCSLELKEKLETSHLKFDASLRRLQELGKDSFLNMDNKFETYQKRLSIDYREILHCLDNLGLICAHLAAEVCLEKISDTKEESETYKECSMVCKEFLEDILSTIGVYLPQDDKSLVDLQQNHLSAVISGHVSPKLKELFHLLDSFRGDKQKQCLILVERIITAKVIERFVKKEASLAYLNVLYLTENNPSTNVSAQKMQIEIPDLFQHGKVNLLFITDVVEEGFQVPDCSCMVCFDLPKTMCSYSQSQKHAKQSNSKSIMFLERGNPKQRDHLHDLMRREVLIQDPEAPNLKSCPPPVKNGHGVKEIGSMVIPDSNITVSEEAASTQTMSDPPSRNEQLPPCKKLRLDNNLLQSNGKEKVASSKSKSSSSAAGSKKRKELHGTTCANALSGTWGENIDGATFQAYKFDFCCNISGEVYSSFSLLLESTLAEDVGKVEMDLYLVRKLVKASVSPCGQIRLSQEELVKAKYFQQFFFNGMFGKLFVGSKSQGTKREFLLQTDTSSLWHPAFMFLLLPVETNDLASSATIDWSAINSCASIVEFLKKNSLLDLRDSDGNQCNTSSGQEVLLDDKMEETNLIHFANASSDKNSLEELVVIAIHTGRIYSIVEAVSDSSAMSPFEVDASSGYATYAEYFNKKYGIVLAHPNQPLMKLKQSHHAHNLLVDFNEEMVVKTEPKAGNVRKRKPNIHAHLPPELLARIDVPRAVLKSIYLLPSVMHRLESLMLASQLREEIDCSIDNFSISSTSILEAVTTLTCPESFSMERLELLGDSVLKYVASCHLFLKYPDKDEGQLSRQRQSIISNSNLHRLTTSRKLQGYIRNGAFEPRRWTAPGQFSLFPVPCKCGIDTREVPLDPKFFTENMTIKIGKSCDMGHRWVVSKSVSDCAEALIGAYYVSGGLSASLHMMKWLGIDVDFDPNLVVEAINRVSLRCYIPKEDELIELERKIQHEFSAKFLLKEAITHSSLRESYSYERLEFLGDSVLDFLITRHLFNTYEQTGPGEMTDLRSACVNNENFAQVAVKNNLHTHLQRCATVLETQINDYLMSFQKPDETGRSIPSIQGPKALGDVVESIAGALLIDTRLDLDQVWRVFEPLLSPLVTPDKLQLPPYRELNELCDSLGYFFRVKCSNDGVKAQATIQLQLDDVLLTGDGSEQTNKLALGKAASHLLTQLEKRNISRKTSLGDNQSSMDVNLACNHSDRETLTSETTEIQSIVIPFIGPINMKKGGPRGTLHEFCKKHLWPMPTFDTSEEKSRTPFEFIDGGEKRTSFSSFTSTITLRIPNREAVMYAGEARPDKKSSFDSAVVELLYELERRKIVIIQK

Target ORF information:

RefSeq Version	NM_114260.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana dicer-like 3 (DCL3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_114260.1

ORF Insert Sequence:

ATGCATTCGT CGTTGGAGCC GGAGAAAATG GAGGAAGGTG GGGGAAGCAA TTCGCTTAAG 
AGAAAATTCT CTGAAATCGA TGGAGATCAA AATCTTGATT CTGTCTCTTC TCCTATGATG 
ACTGACTCTA ATGGTAGTTA TGAATTGAAA GTGTACGAGG TTGCTAAGAA CAGGAACATA 
ATTGCTGTTT TGGGGACAGG GATTGATAAG TCAGAGATCA CTAAGAGGCT TATCAAAGCT 
ATGGGTTCTT CTGATACAGA CAAAAGATTG ATAATTTTCT TGGCCCCAAC TGTGAATCTT 
CAATGCTGTG AGATCAGAGC ACTTGTGAAT TTGAAAGTTG AAGAGTACTT TGGAGCTAAA 
GGAGTTGATA AATGGACATC TCAGCGCTGG GATGAGGAAT TTAGCAAGCA CGATGTTTTA 
GTTATGACTC CTCAAATATT ATTGGATGTC CTTAGAAGTG CATTCCTGAA ACTAGAGATG 
GTATGTCTTC TAATAATAGA TGAATGCCAC CATACCACTG GCAATCATCC CTATGCGAAG 
TTAATGAAGA TTTTTAATCC TGAAGAGCGT GAAGGAGTGG AAAAGTTTGC TACAACGGTT 
AAAGAAGGTC CAATATTGTA TAACCCATCA CCATCCTGTA GTTTGGAATT GAAAGAAAAG 
TTAGAAACTT CACACCTCAA GTTTGATGCT TCTCTTAGAA GGCTTCAAGA GTTGGGAAAA 
GACAGTTTTC TGAATATGGA TAATAAGTTT GAGACATATC AAAAGAGATT GTCTATCGAC 
TACAGAGAGA TTTTGCATTG CCTTGATAAT CTTGGCCTGA TTTGCGCACA CTTGGCGGCT 
GAAGTCTGCT TGGAGAAAAT CTCAGATACG AAAGAGGAAA GTGAAACTTA TAAAGAATGC 
TCAATGGTGT GCAAGGAATT TCTTGAGGAT ATTTTATCCA CCATTGGGGT GTATTTGCCG 
CAAGATGATA AGAGTCTGGT AGATTTGCAG CAAAACCATC TGTCAGCAGT AATTTCTGGG 
CATGTATCTC CAAAGCTAAA AGAACTCTTC CATCTATTGG ATTCCTTTAG AGGTGACAAG 
CAAAAGCAGT GCCTTATTTT AGTTGAGAGA ATTATAACTG CGAAAGTGAT CGAAAGATTC 
GTTAAGAAAG AAGCCTCTTT GGCTTACCTT AATGTCTTGT ATTTAACCGA AAACAACCCC 
TCCACCAATG TATCGGCACA GAAAATGCAA ATTGAAATCC CTGATTTATT TCAACATGGC 
AAGGTGAATC TTTTATTCAT CACAGATGTG GTTGAAGAGG GATTTCAGGT TCCAGATTGC 
TCATGCATGG TTTGTTTTGA CCTGCCCAAA ACAATGTGTA GTTACTCGCA GTCTCAAAAA 
CATGCCAAAC AGAGTAATTC TAAGTCTATC ATGTTTCTTG AAAGAGGGAA CCCGAAGCAA 
AGAGACCATC TGCATGACCT TATGCGAAGA GAAGTCCTAA TTCAAGATCC AGAAGCTCCA 
AACTTGAAAT CGTGTCCACC TCCAGTGAAA AATGGACACG GTGTGAAGGA GATTGGATCC 
ATGGTTATCC CAGATTCTAA CATAACTGTA TCTGAGGAAG CAGCTTCCAC ACAAACTATG 
AGTGATCCTC CTAGCAGAAA TGAGCAGTTA CCACCGTGTA AAAAGTTACG CTTGGATAAC 
AATCTCTTAC AATCCAACGG CAAAGAGAAG GTTGCCTCTT CTAAAAGTAA ATCATCTTCA 
TCGGCTGCAG GTTCAAAAAA ACGTAAGGAG TTGCACGGAA CAACCTGTGC AAACGCATTG 
TCAGGAACCT GGGGAGAAAA TATTGATGGC GCCACCTTTC AGGCTTATAA GTTTGACTTC 
TGTTGTAATA TTTCTGGCGA AGTATACTCG AGTTTCTCTC TTTTGCTTGA GTCAACTCTC 
GCCGAGGATG TTGGTAAAGT TGAGATGGAC CTTTACTTGG TCAGGAAGCT TGTCAAGGCT 
TCTGTCTCAC CTTGTGGCCA GATACGTTTG AGTCAAGAGG AGCTGGTCAA AGCAAAATAT 
TTTCAGCAGT TTTTCTTTAA TGGCATGTTT GGAAAGTTGT TTGTTGGATC TAAGTCACAG 
GGAACAAAGA GAGAATTTTT GCTTCAAACT GACACTAGTT CTCTTTGGCA CCCTGCCTTT 
ATGTTTCTAC TGCTACCAGT TGAAACAAAT GATCTAGCTT CGAGTGCGAC AATTGATTGG 
TCAGCTATCA ACTCCTGTGC CTCAATAGTT GAGTTCTTGA AGAAAAATTC TCTTCTTGAT 
CTTCGGGATA GTGATGGGAA TCAGTGCAAT ACCTCATCCG GTCAGGAAGT CTTACTAGAC 
GATAAAATGG AAGAAACGAA TCTGATTCAT TTTGCCAATG CTTCGTCTGA TAAAAATAGT 
CTCGAAGAAC TTGTGGTCAT TGCAATTCAT ACTGGACGGA TATACTCTAT AGTTGAAGCC 
GTAAGCGATT CTTCTGCTAT GAGCCCCTTT GAGGTGGATG CCTCATCAGG CTATGCTACT 
TATGCAGAAT ATTTTAACAA AAAGTATGGG ATTGTTTTAG CGCACCCGAA CCAGCCGTTG 
ATGAAGTTGA AGCAGAGTCA CCATGCGCAC AACCTTTTAG TCGACTTCAA TGAAGAGATG 
GTTGTGAAGA CAGAACCAAA AGCTGGCAAT GTTAGGAAAA GAAAACCGAA TATCCATGCG 
CATTTGCCTC CAGAGCTTTT GGCTAGAATT GATGTACCGC GTGCTGTGCT AAAATCAATC 
TACTTGCTGC CTTCAGTGAT GCACCGCCTA GAGTCTCTAA TGTTGGCCAG CCAGCTTAGG 
GAAGAGATTG ATTGTAGCAT AGATAACTTC AGTATATCAA GTACATCGAT TCTTGAAGCA 
GTTACAACAC TTACATGCCC CGAATCATTT TCAATGGAGC GGTTGGAACT GCTCGGGGAT 
TCAGTCTTGA AGTATGTTGC GAGCTGTCAT CTATTCCTTA AGTATCCTGA CAAAGATGAG 
GGGCAACTAT CACGGCAGAG ACAATCGATT ATATCTAACT CAAATCTTCA CCGCTTGACA 
ACCAGTCGCA AACTACAGGG ATACATAAGA AATGGCGCTT TTGAACCGCG TCGCTGGACT 
GCACCTGGTC AATTTTCTCT TTTTCCTGTT CCTTGCAAGT GTGGGATTGA TACTAGAGAA 
GTACCATTGG ACCCAAAATT CTTCACAGAA AACATGACTA TCAAAATAGG CAAGTCTTGC 
GACATGGGTC ATAGATGGGT AGTTTCAAAA TCTGTATCAG ATTGCGCTGA GGCCCTGATT 
GGTGCCTATT ATGTAAGCGG TGGATTGTCT GCTTCTCTCC ATATGATGAA ATGGCTCGGT 
ATTGACGTCG ATTTTGACCC AAACCTAGTC GTTGAAGCCA TCAATAGAGT TTCTCTACGG 
TGTTACATTC CTAAAGAAGA TGAGCTCATA GAGTTGGAGA GAAAGATCCA ACATGAATTC 
TCTGCAAAGT TTCTTTTAAA AGAGGCTATC ACACACTCCT CTCTTCGTGA ATCCTATTCA 
TACGAGAGAT TAGAGTTTCT TGGCGATTCT GTACTGGATT TTCTAATAAC CCGTCATCTT 
TTTAACACCT ACGAACAAAC TGGGCCTGGA GAGATGACCG ATCTTCGTTC TGCATGTGTA 
AACAATGAAA ATTTTGCGCA AGTTGCAGTG AAAAATAACC TGCATACCCA CCTTCAACGC 
TGTGCTACGG TTCTCGAGAC TCAAATAAAC GACTATCTGA TGTCCTTTCA AAAGCCAGAT 
GAGACTGGTA GATCAATCCC TTCAATACAG GGCCCTAAGG CTCTTGGAGA TGTTGTGGAG 
AGTATCGCTG GAGCATTGCT GATCGATACG AGGTTAGATC TCGATCAAGT GTGGAGAGTC 
TTTGAGCCGT TGCTTTCTCC ACTTGTAACT CCAGATAAAC TTCAGCTTCC TCCATACCGG 
GAGCTCAATG AGCTATGCGA CTCTCTTGGG TATTTCTTTC GAGTGAAATG TTCAAATGAT 
GGTGTCAAAG CACAAGCCAC GATCCAGTTG CAGCTGGATG ATGTTCTTTT AACTGGAGAT 
GGATCTGAAC AGACAAATAA ACTGGCCTTG GGAAAAGCAG CTTCACATCT GCTTACACAA 
CTTGAGAAGA GAAACATTTC ACGTAAAACC TCGCTCGGGG ATAATCAAAG TTCCATGGAT 
GTCAATCTTG CTTGCAATCA TAGCGACAGA GAAACTCTGA CTTCAGAGAC TACTGAAATC 
CAGAGTATAG TGATTCCATT TATTGGACCT ATAAACATGA AGAAAGGCGG GCCTCGTGGA 
ACTCTACATG AGTTTTGCAA GAAGCATCTG TGGCCAATGC CTACTTTCGA TACCTCGGAA 
GAGAAATCCA GAACTCCGTT TGAATTCATA GATGGCGGTG AGAAGCGGAC TAGCTTCAGC 
AGTTTCACAT CGACCATAAC CCTAAGGATA CCCAATCGTG AGGCTGTGAT GTATGCTGGA 
GAAGCAAGGC CTGACAAGAA GAGTTCCTTC GACTCTGCAG TCGTGGAATT GCTTTATGAG 
CTCGAGCGCC GCAAGATCGT CATAATACAA AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCL3 ( NM_001161191.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001154663.2 Arabidopsis thaliana dicer-like 3 (DCL3), mRNA.

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CloneID	OAb29531
Clone ID Related Accession (Same CDS sequence)	NM_001161191.3
Accession Version	NM_001161191.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	dicer-like 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_001161191.2.

ATGCATTCGT CGTTGGAGCC GGAGAAAATG GAGGAAGGTG GGGGAAGCAA TTCGCTTAAG 
AGAAAATTCT CTGAAATCGA TGGAGATCAA AATCTTGATT CTGTCTCTTC TCCTATGATG 
ACTGACTCTA ATGGTAGTTA TGAATTGAAA GTGTACGAGG TTGCTAAGAA CAGGAACATA 
ATTGCTGTTT TGGGGACAGG GATTGATAAG TCAGAGATCA CTAAGAGGCT TATCAAAGCT 
ATGGGTTCTT CTGATACAGA CAAAAGATTG ATAATTTTCT TGGCCCCAAC TGTGAATCTT 
GTTAAACAGC AATGCTGTGA GATCAGAGCA CTTGTGAATT TGAAAGTTGA AGAGTACTTT 
GGAGCTAAAG GAGTTGATAA ATGGACATCT CAGCGCTGGG ATGAGGAATT TAGCAAGCAC 
GATGTTTTAG TTATGACTCC TCAAATATTA TTGGATGTCC TTAGAAGTGC ATTCCTGAAA 
CTAGAGATGG TATGTCTTCT AATAATAGAT GAATGCCACC ATACCACTGG CAATCATCCC 
TATGCGAAGT TAATGAAGGA ATTCTATCAC GAATCCACTA GCAAACCGAA GATATTTGGA 
TTGACTGCGT CAGCCGTCAT TAGAAAAGCT CAAGTATCAG AACTTGAGAG ACTCATGGAC 
TCAAAGATTT TTAATCCTGA AGAGCGTGAA GGAGTGGAAA AGTTTGCTAC AACGGTTAAA 
GAAGGTCCAA TATTGTATAA CCCATCACCA TCCTGTAGTT TGGAATTGAA AGAAAAGTTA 
GAAACTTCAC ACCTCAAGTT TGATGCTTCT CTTAGAAGGC TTCAAGAGTT GGGAAAAGAC 
AGTTTTCTGA ATATGGATAA TAAGTTTGAG ACATATCAAA AGAGATTGTC TATCGACTAC 
AGAGAGATTT TGCATTGCCT TGATAATCTT GGCCTGATTT GCGCACACTT GGCGGCTGAA 
GTCTGCTTGG AGAAAATCTC AGATACGAAA GAGGAAAGTG AAACTTATAA AGAATGCTCA 
ATGGTGTGCA AGGAATTTCT TGAGGATATT TTATCCACCA TTGGGGTGTA TTTGCCGCAA 
GATGATAAGA GTCTGGTAGA TTTGCAGCAA AACCATCTGT CAGCAGTAAT TTCTGGGCAT 
GTATCTCCAA AGCTAAAAGA ACTCTTCCAT CTATTGGATT CCTTTAGAGG TGACAAGCAA 
AAGCAGTGCC TTATTTTAGT TGAGAGAATT ATAACTGCGA AAGTGATCGA AAGATTCGTT 
AAGAAAGAAG CCTCTTTGGC TTACCTTAAT GTCTTGTATT TAACCGAAAA CAACCCCTCC 
ACCAATGTAT CGGCACAGAA AATGCAAATT GAAATCCCTG ATTTATTTCA ACATGGCAAG 
GTGAATCTTT TATTCATCAC AGATGTGGTT GAAGAGGGAT TTCAGGTTCC AGATTGCTCA 
TGCATGGTTT GTTTTGACCT GCCCAAAACA ATGTGTAGTT ACTCGCAGTC TCAAAAACAT 
GCCAAACAGA GTAATTCTAA GTCTATCATG TTTCTTGAAA GAGGGAACCC GAAGCAAAGA 
GACCATCTGC ATGACCTTAT GCGAAGAGAA GTCCTAATTC AAGATCCAGA AGCTCCAAAC 
TTGAAATCGT GTCCACCTCC AGTGAAAAAT GGACACGGTG TGAAGGAGAT TGGATCCATG 
GTTATCCCAG ATTCTAACAT AACTGTATCT GAGGAAGCAG CTTCCACACA AACTATGAGT 
GATCCTCCTA GCAGAAATGA GCAGTTACCA CCGTGTAAAA AGTTACGCTT GGATAACAAT 
CTCTTACAAT CCAACGGCAA AGAGAAGGTT GCCTCTTCTA AAAGTAAATC ATCTTCATCG 
GCTGCAGGTT CAAAAAAACG TAAGGAGTTG CACGGAACAA CCTGTGCAAA CGCATTGTCA 
GGAACCTGGG GAGAAAATAT TGATGGCGCC ACCTTTCAGG CTTATAAGTT TGACTTCTGT 
TGTAATATTT CTGGCGAAGT ATACTCGAGT TTCTCTCTTT TGCTTGAGTC AACTCTCGCC 
GAGGATGTTG GTAAAGTTGA GATGGACCTT TACTTGGTCA GGAAGCTTGT CAAGGCTTCT 
GTCTCACCTT GTGGCCAGAT ACGTTTGAGT CAAGAGGAGC TGGTCAAAGC AAAATATTTT 
CAGCAGTTTT TCTTTAATGG CATGTTTGGA AAGTTGTTTG TTGGATCTAA GTCACAGGGA 
ACAAAGAGAG AATTTTTGCT TCAAACTGAC ACTAGTTCTC TTTGGCACCC TGCCTTTATG 
TTTCTACTGC TACCAGTTGA AACAAATGAT CTAGCTTCGA GTGCGACAAT TGATTGGTCA 
GCTATCAACT CCTGTGCCTC AATAGTTGAG TTCTTGAAGA AAAATTCTCT TCTTGATCTT 
CGGGATAGTG ATGGGAATCA GTGCAATACC TCATCCGGTC AGGAAGTCTT ACTAGACGAT 
AAAATGGAAG AAACGAATCT GATTCATTTT GCCAATGCTT CGTCTGATAA AAATAGTCTC 
GAAGAACTTG TGGTCATTGC AATTCATACT GGACGGATAT ACTCTATAGT TGAAGCCGTA 
AGCGATTCTT CTGCTATGAG CCCCTTTGAG GTGGATGCCT CATCAGGCTA TGCTACTTAT 
GCAGAATATT TTAACAAAAA GTATGGGATT GTTTTAGCGC ACCCGAACCA GCCGTTGATG 
AAGTTGAAGC AGAGTCACCA TGCGCACAAC CTTTTAGTCG ACTTCAATGA AGAGATGGTT 
GTGAAGACAG AACCAAAAGC TGGCAATGTT AGGAAAAGAA AACCGAATAT CCATGCGCAT 
TTGCCTCCAG AGCTTTTGGC TAGAATTGAT GTACCGCGTG CTGTGCTAAA ATCAATCTAC 
TTGCTGCCTT CAGTGATGCA CCGCCTAGAG TCTCTAATGT TGGCCAGCCA GCTTAGGGAA 
GAGATTGATT GTAGCATAGA TAACTTCAGT ATATCAAGTA CATCGATTCT TGAAGCAGTT 
ACAACACTTA CATGCCCCGA ATCATTTTCA ATGGAGCGGT TGGAACTGCT CGGGGATTCA 
GTCTTGAAGT ATGTTGCGAG CTGTCATCTA TTCCTTAAGT ATCCTGACAA AGATGAGGGG 
CAACTATCAC GGCAGAGACA ATCGATTATA TCTAACTCAA ATCTTCACCG CTTGACAACC 
AGTCGCAAAC TACAGGGATA CATAAGAAAT GGCGCTTTTG AACCGCGTCG CTGGACTGCA 
CCTGGTCAAT TTTCTCTTTT TCCTGTTCCT TGCAAGTGTG GGATTGATAC TAGAGAAGTA 
CCATTGGACC CAAAATTCTT CACAGAAAAC ATGACTATCA AAATAGGCAA GTCTTGCGAC 
ATGGGTCATA GATGGGTAGT TTCAAAATCT GTATCAGATT GCGCTGAGGC CCTGATTGGT 
GCCTATTATG TAAGCGGTGG ATTGTCTGCT TCTCTCCATA TGATGAAATG GCTCGGTATT 
GACGTCGATT TTGACCCAAA CCTAGTCGTT GAAGCCATCA ATAGAGTTTC TCTACGGTGT 
TACATTCCTA AAGAAGATGA GCTCATAGAG TTGGAGAGAA AGATCCAACA TGAATTCTCT 
GCAAAGTTTC TTTTAAAAGA GGCTATCACA CACTCCTCTC TTCGTGAATC CTATTCATAC 
GAGAGATTAG AGTTTCTTGG CGATTCTGTA CTGGATTTTC TAATAACCCG TCATCTTTTT 
AACACCTACG AACAAACTGG GCCTGGAGAG ATGACCGATC TTCGTTCTGC ATGTGTAAAC 
AATGAAAATT TTGCGCAAGT TGCAGTGAAA AATAACCTGC ATACCCACCT TCAACGCTGT 
GCTACGGTTC TCGAGACTCA AATAAACGAC TATCTGATGT CCTTTCAAAA GCCAGATGAG 
ACTGGTAGAT CAATCCCTTC AATACAGGGC CCTAAGGCTC TTGGAGATGT TGTGGAGAGT 
ATCGCTGGAG CATTGCTGAT CGATACGAGG TTAGATCTCG ATCAAGTGTG GAGAGTCTTT 
GAGCCGTTGC TTTCTCCACT TGTAACTCCA GATAAACTTC AGCTTCCTCC ATACCGGGAG 
CTCAATGAGC TATGCGACTC TCTTGGGTAT TTCTTTCGAG TGAAATGTTC AAATGATGGT 
GTCAAAGCAC AAGCCACGAT CCAGTTGCAG CTGGATGATG TTCTTTTAAC TGGAGATGGA 
TCTGAACAGA CAAATAAACT GGCCTTGGGA AAAGCAGCTT CACATCTGCT TACACAACTT 
GAGAAGAGAA ACATTTCACG TAAAACCTCG CTCGGGGATA ATCAAAGTTC CATGGATGTC 
AATCTTGCTT GCAATCATAG CGACAGAGAA ACTCTGACTT CAGAGACTAC TGAAATCCAG 
AGTATAGTGA TTCCATTTAT TGGACCTATA AACATGAAGA AAGGCGGGCC TCGTGGAACT 
CTACATGAGT TTTGCAAGAA GCATCTGTGG CCAATGCCTA CTTTCGATAC CTCGGAAGAG 
AAATCCAGAA CTCCGTTTGA ATTCATAGAT GGCGGTGAGA AGCGGACTAG CTTCAGCAGT 
TTCACATCGA CCATAACCCT AAGGATACCC AATCGTGAGG CTGTGATGTA TGCTGGAGAA 
GCAAGGCCTG ACAAGAAGAG TTCCTTCGAC TCTGCAGTCG TGGAATTGCT TTATGAGCTC 
GAGCGCCGCA AGATCGTCAT AATACAAAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001154663.2
CDS	149..4861
Translation	MHSSLEPEKMEEGGGSNSLKRKFSEIDGDQNLDSVSSPMMTDSNGSYELKVYEVAKNRNIIAVLGTGIDKSEITKRLIKAMGSSDTDKRLIIFLAPTVNLVKQQCCEIRALVNLKVEEYFGAKGVDKWTSQRWDEEFSKHDVLVMTPQILLDVLRSAFLKLEMVCLLIIDECHHTTGNHPYAKLMKEFYHESTSKPKIFGLTASAVIRKAQVSELERLMDSKIFNPEEREGVEKFATTVKEGPILYNPSPSCSLELKEKLETSHLKFDASLRRLQELGKDSFLNMDNKFETYQKRLSIDYREILHCLDNLGLICAHLAAEVCLEKISDTKEESETYKECSMVCKEFLEDILSTIGVYLPQDDKSLVDLQQNHLSAVISGHVSPKLKELFHLLDSFRGDKQKQCLILVERIITAKVIERFVKKEASLAYLNVLYLTENNPSTNVSAQKMQIEIPDLFQHGKVNLLFITDVVEEGFQVPDCSCMVCFDLPKTMCSYSQSQKHAKQSNSKSIMFLERGNPKQRDHLHDLMRREVLIQDPEAPNLKSCPPPVKNGHGVKEIGSMVIPDSNITVSEEAASTQTMSDPPSRNEQLPPCKKLRLDNNLLQSNGKEKVASSKSKSSSSAAGSKKRKELHGTTCANALSGTWGENIDGATFQAYKFDFCCNISGEVYSSFSLLLESTLAEDVGKVEMDLYLVRKLVKASVSPCGQIRLSQEELVKAKYFQQFFFNGMFGKLFVGSKSQGTKREFLLQTDTSSLWHPAFMFLLLPVETNDLASSATIDWSAINSCASIVEFLKKNSLLDLRDSDGNQCNTSSGQEVLLDDKMEETNLIHFANASSDKNSLEELVVIAIHTGRIYSIVEAVSDSSAMSPFEVDASSGYATYAEYFNKKYGIVLAHPNQPLMKLKQSHHAHNLLVDFNEEMVVKTEPKAGNVRKRKPNIHAHLPPELLARIDVPRAVLKSIYLLPSVMHRLESLMLASQLREEIDCSIDNFSISSTSILEAVTTLTCPESFSMERLELLGDSVLKYVASCHLFLKYPDKDEGQLSRQRQSIISNSNLHRLTTSRKLQGYIRNGAFEPRRWTAPGQFSLFPVPCKCGIDTREVPLDPKFFTENMTIKIGKSCDMGHRWVVSKSVSDCAEALIGAYYVSGGLSASLHMMKWLGIDVDFDPNLVVEAINRVSLRCYIPKEDELIELERKIQHEFSAKFLLKEAITHSSLRESYSYERLEFLGDSVLDFLITRHLFNTYEQTGPGEMTDLRSACVNNENFAQVAVKNNLHTHLQRCATVLETQINDYLMSFQKPDETGRSIPSIQGPKALGDVVESIAGALLIDTRLDLDQVWRVFEPLLSPLVTPDKLQLPPYRELNELCDSLGYFFRVKCSNDGVKAQATIQLQLDDVLLTGDGSEQTNKLALGKAASHLLTQLEKRNISRKTSLGDNQSSMDVNLACNHSDRETLTSETTEIQSIVIPFIGPINMKKGGPRGTLHEFCKKHLWPMPTFDTSEEKSRTPFEFIDGGEKRTSFSSFTSTITLRIPNREAVMYAGEARPDKKSSFDSAVVELLYELERRKIVIIQK

Target ORF information:

RefSeq Version	NM_001161191.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana dicer-like 3 (DCL3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161191.3

ORF Insert Sequence:

ATGCATTCGT CGTTGGAGCC GGAGAAAATG GAGGAAGGTG GGGGAAGCAA TTCGCTTAAG 
AGAAAATTCT CTGAAATCGA TGGAGATCAA AATCTTGATT CTGTCTCTTC TCCTATGATG 
ACTGACTCTA ATGGTAGTTA TGAATTGAAA GTGTACGAGG TTGCTAAGAA CAGGAACATA 
ATTGCTGTTT TGGGGACAGG GATTGATAAG TCAGAGATCA CTAAGAGGCT TATCAAAGCT 
ATGGGTTCTT CTGATACAGA CAAAAGATTG ATAATTTTCT TGGCCCCAAC TGTGAATCTT 
GTTAAACAGC AATGCTGTGA GATCAGAGCA CTTGTGAATT TGAAAGTTGA AGAGTACTTT 
GGAGCTAAAG GAGTTGATAA ATGGACATCT CAGCGCTGGG ATGAGGAATT TAGCAAGCAC 
GATGTTTTAG TTATGACTCC TCAAATATTA TTGGATGTCC TTAGAAGTGC ATTCCTGAAA 
CTAGAGATGG TATGTCTTCT AATAATAGAT GAATGCCACC ATACCACTGG CAATCATCCC 
TATGCGAAGT TAATGAAGGA ATTCTATCAC GAATCCACTA GCAAACCGAA GATATTTGGA 
TTGACTGCGT CAGCCGTCAT TAGAAAAGCT CAAGTATCAG AACTTGAGAG ACTCATGGAC 
TCAAAGATTT TTAATCCTGA AGAGCGTGAA GGAGTGGAAA AGTTTGCTAC AACGGTTAAA 
GAAGGTCCAA TATTGTATAA CCCATCACCA TCCTGTAGTT TGGAATTGAA AGAAAAGTTA 
GAAACTTCAC ACCTCAAGTT TGATGCTTCT CTTAGAAGGC TTCAAGAGTT GGGAAAAGAC 
AGTTTTCTGA ATATGGATAA TAAGTTTGAG ACATATCAAA AGAGATTGTC TATCGACTAC 
AGAGAGATTT TGCATTGCCT TGATAATCTT GGCCTGATTT GCGCACACTT GGCGGCTGAA 
GTCTGCTTGG AGAAAATCTC AGATACGAAA GAGGAAAGTG AAACTTATAA AGAATGCTCA 
ATGGTGTGCA AGGAATTTCT TGAGGATATT TTATCCACCA TTGGGGTGTA TTTGCCGCAA 
GATGATAAGA GTCTGGTAGA TTTGCAGCAA AACCATCTGT CAGCAGTAAT TTCTGGGCAT 
GTATCTCCAA AGCTAAAAGA ACTCTTCCAT CTATTGGATT CCTTTAGAGG TGACAAGCAA 
AAGCAGTGCC TTATTTTAGT TGAGAGAATT ATAACTGCGA AAGTGATCGA AAGATTCGTT 
AAGAAAGAAG CCTCTTTGGC TTACCTTAAT GTCTTGTATT TAACCGAAAA CAACCCCTCC 
ACCAATGTAT CGGCACAGAA AATGCAAATT GAAATCCCTG ATTTATTTCA ACATGGCAAG 
GTGAATCTTT TATTCATCAC AGATGTGGTT GAAGAGGGAT TTCAGGTTCC AGATTGCTCA 
TGCATGGTTT GTTTTGACCT GCCCAAAACA ATGTGTAGTT ACTCGCAGTC TCAAAAACAT 
GCCAAACAGA GTAATTCTAA GTCTATCATG TTTCTTGAAA GAGGGAACCC GAAGCAAAGA 
GACCATCTGC ATGACCTTAT GCGAAGAGAA GTCCTAATTC AAGATCCAGA AGCTCCAAAC 
TTGAAATCGT GTCCACCTCC AGTGAAAAAT GGACACGGTG TGAAGGAGAT TGGATCCATG 
GTTATCCCAG ATTCTAACAT AACTGTATCT GAGGAAGCAG CTTCCACACA AACTATGAGT 
GATCCTCCTA GCAGAAATGA GCAGTTACCA CCGTGTAAAA AGTTACGCTT GGATAACAAT 
CTCTTACAAT CCAACGGCAA AGAGAAGGTT GCCTCTTCTA AAAGTAAATC ATCTTCATCG 
GCTGCAGGTT CAAAAAAACG TAAGGAGTTG CACGGAACAA CCTGTGCAAA CGCATTGTCA 
GGAACCTGGG GAGAAAATAT TGATGGCGCC ACCTTTCAGG CTTATAAGTT TGACTTCTGT 
TGTAATATTT CTGGCGAAGT ATACTCGAGT TTCTCTCTTT TGCTTGAGTC AACTCTCGCC 
GAGGATGTTG GTAAAGTTGA GATGGACCTT TACTTGGTCA GGAAGCTTGT CAAGGCTTCT 
GTCTCACCTT GTGGCCAGAT ACGTTTGAGT CAAGAGGAGC TGGTCAAAGC AAAATATTTT 
CAGCAGTTTT TCTTTAATGG CATGTTTGGA AAGTTGTTTG TTGGATCTAA GTCACAGGGA 
ACAAAGAGAG AATTTTTGCT TCAAACTGAC ACTAGTTCTC TTTGGCACCC TGCCTTTATG 
TTTCTACTGC TACCAGTTGA AACAAATGAT CTAGCTTCGA GTGCGACAAT TGATTGGTCA 
GCTATCAACT CCTGTGCCTC AATAGTTGAG TTCTTGAAGA AAAATTCTCT TCTTGATCTT 
CGGGATAGTG ATGGGAATCA GTGCAATACC TCATCCGGTC AGGAAGTCTT ACTAGACGAT 
AAAATGGAAG AAACGAATCT GATTCATTTT GCCAATGCTT CGTCTGATAA AAATAGTCTC 
GAAGAACTTG TGGTCATTGC AATTCATACT GGACGGATAT ACTCTATAGT TGAAGCCGTA 
AGCGATTCTT CTGCTATGAG CCCCTTTGAG GTGGATGCCT CATCAGGCTA TGCTACTTAT 
GCAGAATATT TTAACAAAAA GTATGGGATT GTTTTAGCGC ACCCGAACCA GCCGTTGATG 
AAGTTGAAGC AGAGTCACCA TGCGCACAAC CTTTTAGTCG ACTTCAATGA AGAGATGGTT 
GTGAAGACAG AACCAAAAGC TGGCAATGTT AGGAAAAGAA AACCGAATAT CCATGCGCAT 
TTGCCTCCAG AGCTTTTGGC TAGAATTGAT GTACCGCGTG CTGTGCTAAA ATCAATCTAC 
TTGCTGCCTT CAGTGATGCA CCGCCTAGAG TCTCTAATGT TGGCCAGCCA GCTTAGGGAA 
GAGATTGATT GTAGCATAGA TAACTTCAGT ATATCAAGTA CATCGATTCT TGAAGCAGTT 
ACAACACTTA CATGCCCCGA ATCATTTTCA ATGGAGCGGT TGGAACTGCT CGGGGATTCA 
GTCTTGAAGT ATGTTGCGAG CTGTCATCTA TTCCTTAAGT ATCCTGACAA AGATGAGGGG 
CAACTATCAC GGCAGAGACA ATCGATTATA TCTAACTCAA ATCTTCACCG CTTGACAACC 
AGTCGCAAAC TACAGGGATA CATAAGAAAT GGCGCTTTTG AACCGCGTCG CTGGACTGCA 
CCTGGTCAAT TTTCTCTTTT TCCTGTTCCT TGCAAGTGTG GGATTGATAC TAGAGAAGTA 
CCATTGGACC CAAAATTCTT CACAGAAAAC ATGACTATCA AAATAGGCAA GTCTTGCGAC 
ATGGGTCATA GATGGGTAGT TTCAAAATCT GTATCAGATT GCGCTGAGGC CCTGATTGGT 
GCCTATTATG TAAGCGGTGG ATTGTCTGCT TCTCTCCATA TGATGAAATG GCTCGGTATT 
GACGTCGATT TTGACCCAAA CCTAGTCGTT GAAGCCATCA ATAGAGTTTC TCTACGGTGT 
TACATTCCTA AAGAAGATGA GCTCATAGAG TTGGAGAGAA AGATCCAACA TGAATTCTCT 
GCAAAGTTTC TTTTAAAAGA GGCTATCACA CACTCCTCTC TTCGTGAATC CTATTCATAC 
GAGAGATTAG AGTTTCTTGG CGATTCTGTA CTGGATTTTC TAATAACCCG TCATCTTTTT 
AACACCTACG AACAAACTGG GCCTGGAGAG ATGACCGATC TTCGTTCTGC ATGTGTAAAC 
AATGAAAATT TTGCGCAAGT TGCAGTGAAA AATAACCTGC ATACCCACCT TCAACGCTGT 
GCTACGGTTC TCGAGACTCA AATAAACGAC TATCTGATGT CCTTTCAAAA GCCAGATGAG 
ACTGGTAGAT CAATCCCTTC AATACAGGGC CCTAAGGCTC TTGGAGATGT TGTGGAGAGT 
ATCGCTGGAG CATTGCTGAT CGATACGAGG TTAGATCTCG ATCAAGTGTG GAGAGTCTTT 
GAGCCGTTGC TTTCTCCACT TGTAACTCCA GATAAACTTC AGCTTCCTCC ATACCGGGAG 
CTCAATGAGC TATGCGACTC TCTTGGGTAT TTCTTTCGAG TGAAATGTTC AAATGATGGT 
GTCAAAGCAC AAGCCACGAT CCAGTTGCAG CTGGATGATG TTCTTTTAAC TGGAGATGGA 
TCTGAACAGA CAAATAAACT GGCCTTGGGA AAAGCAGCTT CACATCTGCT TACACAACTT 
GAGAAGAGAA ACATTTCACG TAAAACCTCG CTCGGGGATA ATCAAAGTTC CATGGATGTC 
AATCTTGCTT GCAATCATAG CGACAGAGAA ACTCTGACTT CAGAGACTAC TGAAATCCAG 
AGTATAGTGA TTCCATTTAT TGGACCTATA AACATGAAGA AAGGCGGGCC TCGTGGAACT 
CTACATGAGT TTTGCAAGAA GCATCTGTGG CCAATGCCTA CTTTCGATAC CTCGGAAGAG 
AAATCCAGAA CTCCGTTTGA ATTCATAGAT GGCGGTGAGA AGCGGACTAG CTTCAGCAGT 
TTCACATCGA CCATAACCCT AAGGATACCC AATCGTGAGG CTGTGATGTA TGCTGGAGAA 
GCAAGGCCTG ACAAGAAGAG TTCCTTCGAC TCTGCAGTCG TGGAATTGCT TTATGAGCTC 
GAGCGCCGCA AGATCGTCAT AATACAAAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCL3 ( NM_001161190.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001154662.2 Arabidopsis thaliana dicer-like 3 (DCL3), partial mRNA.

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CloneID	OAb29532
Clone ID Related Accession (Same CDS sequence)	NM_001161190.2
Accession Version	NM_001161190.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	dicer-like 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On May 26, 2011 this sequence version replaced NM_001161190.1. COMPLETENESS: incomplete on both ends.

ATGCATTCGT CGTTGGAGCC GGAGAAAATG GAGGAAGGTG GGGGAAGCAA TTCGCTTAAG 
AGAAAATTCT CTGAAATCGA TGGAGATCAA AATCTTGATT CTGTCTCTTC TCCTATGATG 
ACTGACTCTA ATGGTAGTTA TGAATTGAAA GTGTACGAGG TTGCTAAGAA CAGGAACATA 
ATTGCTGTTT TGGGGACAGG GATTGATAAG TCAGAGATCA CTAAGAGGCT TATCAAAGCT 
ATGGGTTCTT CTGATACAGA CAAAAGATTG ATAATTTTCT TGGCCCCAAC TGTGAATCTT 
GTTAAACAGC AATGCTGTGA GATCAGAGCA CTTGTGAATT TGAAAGTTGA AGAGTACTTT 
GGAGCTAAAG GAGTTGATAA ATGGACATCT CAGCGCTGGG ATGAGGAATT TAGCAAGCAC 
GATGTTTTAG TTATGACTCC TCAAATATTA TTGGATGTCC TTAGAAGTGC ATTCCTGAAA 
CTAGAGATGG TATGTCTTCT AATAATAGAT GAATGCCACC ATACCACTGG CAATCATCCC 
TATGCGAAGT TAATGAAGGA ATTCTATCAC GAATCCACTA GCAAACCGAA GATATTTGGA 
TTGACTGCGT CAGCCGTCAT TAGAAAAGGC ATTGTTTCCT CTCCCTCTAA CTATGCAGCT 
CAAGTATCAG AACTTGAGAG ACTCATGGAC TCAAAGATTT TTAATCCTGA AGAGCGTGAA 
GGAGTGGAAA AGTTTGCTAC AACGGTTAAA GAAGGTCCAA TATTGTATAA CCCATCACCA 
TCCTGTAGTT TGGAATTGAA AGAAAAGTTA GAAACTTCAC ACCTCAAGTT TGATGCTTCT 
CTTAGAAGGC TTCAAGAGTT GGGAAAAGAC AGTTTTCTGA ATATGGATAA TAAGTTTGAG 
ACATATCAAA AGAGATTGTC TATCGACTAC AGAGAGATTT TGCATTGCCT TGATAATCTT 
GGCCTGATTT GCGCACACTT GGCGGCTGAA GTCTGCTTGG AGAAAATCTC AGATACGAAA 
GAGGAAAGTG AAACTTATAA AGAATGCTCA ATGGTGTGCA AGGAATTTCT TGAGGATATT 
TTATCCACCA TTGGGGTGTA TTTGCCGCAA GATGATAAGA GTCTGGTAGA TTTGCAGCAA 
AACCATCTGT CAGCAGTAAT TTCTGGGCAT GTATCTCCAA AGCTAAAAGA ACTCTTCCAT 
CTATTGGATT CCTTTAGAGG TGACAAGCAA AAGCAGTGCC TTATTTTAGT TGAGAGAATT 
ATAACTGCGA AAGTGATCGA AAGATTCGTT AAGAAAGAAG CCTCTTTGGC TTACCTTAAT 
GTCTTGTATT TAACCGAAAA CAACCCCTCC ACCAATGTAT CGGCACAGAA AATGCAAATT 
GAAATCCCTG ATTTATTTCA ACATGGCAAG GTGAATCTTT TATTCATCAC AGATGTGGTT 
GAAGAGGGAT TTCAGGTTCC AGATTGCTCA TGCATGGTTT GTTTTGACCT GCCCAAAACA 
ATGTGTAGTT ACTCGCAGTC TCAAAAACAT GCCAAACAGA GTAATTCTAA GTCTATCATG 
TTTCTTGAAA GAGGGAACCC GAAGCAAAGA GACCATCTGC ATGACCTTAT GCGAAGAGAA 
GTCCTAATTC AAGATCCAGA AGCTCCAAAC TTGAAATCGT GTCCACCTCC AGTGAAAAAT 
GGACACGGTG TGAAGGAGAT TGGATCCATG GTTATCCCAG ATTCTAACAT AACTGTATCT 
GAGGAAGCAG CTTCCACACA AACTATGAGT GATCCTCCTA GCAGAAATGA GCAGTTACCA 
CCGTGTAAAA AGTTACGCTT GGATAACAAT CTCTTACAAT CCAACGGCAA AGAGAAGGTT 
GCCTCTTCTA AAAGTAAATC ATCTTCATCG GCTGCAGGTT CAAAAAAACG TAAGGAGTTG 
CACGGAACAA CCTGTGCAAA CGCATTGTCA GGAACCTGGG GAGAAAATAT TGATGGCGCC 
ACCTTTCAGG CTTATAAGTT TGACTTCTGT TGTAATATTT CTGGCGAAGT ATACTCGAGT 
TTCTCTCTTT TGCTTGAGTC AACTCTCGCC GAGGATGTTG GTAAAGTTGA GATGGACCTT 
TACTTGGTCA GGAAGCTTGT CAAGGCTTCT GTCTCACCTT GTGGCCAGAT ACGTTTGAGT 
CAAGAGGAGC TGGTCAAAGC AAAATATTTT CAGCAGTTTT TCTTTAATGG CATGTTTGGA 
AAGTTGTTTG TTGGATCTAA GTCACAGGGA ACAAAGAGAG AATTTTTGCT TCAAACTGAC 
ACTAGTTCTC TTTGGCACCC TGCCTTTATG TTTCTACTGC TACCAGTTGA AACAAATGAT 
CTAGCTTCGA GTGCGACAAT TGATTGGTCA GCTATCAACT CCTGTGCCTC AATAGTTGAG 
TTCTTGAAGA AAAATTCTCT TCTTGATCTT CGGGATAGTG ATGGGAATCA GTGCAATACC 
TCATCCGGTC AGGAAGTCTT ACTAGACGAT AAAATGGAAG AAACGAATCT GATTCATTTT 
GCCAATGCTT CGTCTGATAA AAATAGTCTC GAAGAACTTG TGGTCATTGC AATTCATACT 
GGACGGATAT ACTCTATAGT TGAAGCCGTA AGCGATTCTT CTGCTATGAG CCCCTTTGAG 
GTGGATGCCT CATCAGGCTA TGCTACTTAT GCAGAATATT TTAACAAAAA GTATGGGATT 
GTTTTAGCGC ACCCGAACCA GCCGTTGATG AAGTTGAAGC AGAGTCACCA TGCGCACAAC 
CTTTTAGTCG ACTTCAATGA AGAGATGGTT GTGAAGACAG AACCAAAAGC TGGCAATGTT 
AGGAAAAGAA AACCGAATAT CCATGCGCAT TTGCCTCCAG AGCTTTTGGC TAGAATTGAT 
GTACCGCGTG CTGTGCTAAA ATCAATCTAC TTGCTGCCTT CAGTGATGCA CCGCCTAGAG 
TCTCTAATGT TGGCCAGCCA GCTTAGGGAA GAGATTGATT GTAGCATAGA TAACTTCAGT 
ATATCAAGTA CATCGATTCT TGAAGCAGTT ACAACACTTA CATGCCCCGA ATCATTTTCA 
ATGGAGCGGT TGGAACTGCT CGGGGATTCA GTCTTGAAGT ATGTTGCGAG CTGTCATCTA 
TTCCTTAAGT ATCCTGACAA AGATGAGGGG CAACTATCAC GGCAGAGACA ATCGATTATA 
TCTAACTCAA ATCTTCACCG CTTGACAACC AGTCGCAAAC TACAGGGATA CATAAGAAAT 
GGCGCTTTTG AACCGCGTCG CTGGACTGCA CCTGGTCAAT TTTCTCTTTT TCCTGTTCCT 
TGCAAGTGTG GGATTGATAC TAGAGAAGTA CCATTGGACC CAAAATTCTT CACAGAAAAC 
ATGACTATCA AAATAGGCAA GTCTTGCGAC ATGGGTCATA GATGGGTAGT TTCAAAATCT 
GTATCAGATT GCGCTGAGGC CCTGATTGGT GCCTATTATG TAAGCGGTGG ATTGTCTGCT 
TCTCTCCATA TGATGAAATG GCTCGGTATT GACGTCGATT TTGACCCAAA CCTAGTCGTT 
GAAGCCATCA ATAGAGTTTC TCTACGGTGT TACATTCCTA AAGAAGATGA GCTCATAGAG 
TTGGAGAGAA AGATCCAACA TGAATTCTCT GCAAAGTTTC TTTTAAAAGA GGCTATCACA 
CACTCCTCTC TTCGTGAATC CTATTCATAC GAGAGATTAG AGTTTCTTGG CGATTCTGTA 
CTGGATTTTC TAATAACCCG TCATCTTTTT AACACCTACG AACAAACTGG GCCTGGAGAG 
ATGACCGATC TTCGTTCTGC ATGTGTAAAC AATGAAAATT TTGCGCAAGT TGCAGTGAAA 
AATAACCTGC ATACCCACCT TCAACGCTGT GCTACGGTTC TCGAGACTCA AATAAACGAC 
TATCTGATGT CCTTTCAAAA GCCAGATGAG ACTGGTAGAT CAATCCCTTC AATACAGGGC 
CCTAAGGCTC TTGGAGATGT TGTGGAGAGT ATCGCTGGAG CATTGCTGAT CGATACGAGG 
TTAGATCTCG ATCAAGTGTG GAGAGTCTTT GAGCCGTTGC TTTCTCCACT TGTAACTCCA 
GATAAACTTC AGCTTCCTCC ATACCGGGAG CTCAATGAGC TATGCGACTC TCTTGGGTAT 
TTCTTTCGAG TGAAATGTTC AAATGATGGT GTCAAAGCAC AAGCCACGAT CCAGTTGCAG 
CTGGATGATG TTCTTTTAAC TGGAGATGGA TCTGAACAGA CAAATAAACT GGCCTTGGGA 
AAAGCAGCTT CACATCTGCT TACACAACTT GAGAAGAGAA ACATTTCACG TAAAACCTCG 
CTCGGGGATA ATCAAAGTTC CATGGATGTC AATCTTGCTT GCAATCATAG CGACAGAGAA 
ACTCTGACTT CAGAGACTAC TGAAATCCAG AGTATAGTGA TTCCATTTAT TGGACCTATA 
AACATGAAGA AAGGCGGGCC TCGTGGAACT CTACATGAGT TTTGCAAGAA GCATCTGTGG 
CCAATGCCTA CTTTCGATAC CTCGGAAGAG AAATCCAGAA CTCCGTTTGA ATTCATAGAT 
GGCGGTGAGA AGCGGACTAG CTTCAGCAGT TTCACATCGA CCATAACCCT AAGGATACCC 
AATCGTGAGG CTGTGATGTA TGCTGGAGAA GCAAGGCCTG ACAAGAAGAG TTCCTTCGAC 
TCTGCAGTCG TGGAATTGCT TTATGAGCTC GAGCGCCGCA AGATCGTCAT AATACAAAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001154662.2
CDS	1..4743
Translation	MHSSLEPEKMEEGGGSNSLKRKFSEIDGDQNLDSVSSPMMTDSNGSYELKVYEVAKNRNIIAVLGTGIDKSEITKRLIKAMGSSDTDKRLIIFLAPTVNLVKQQCCEIRALVNLKVEEYFGAKGVDKWTSQRWDEEFSKHDVLVMTPQILLDVLRSAFLKLEMVCLLIIDECHHTTGNHPYAKLMKEFYHESTSKPKIFGLTASAVIRKGIVSSPSNYAAQVSELERLMDSKIFNPEEREGVEKFATTVKEGPILYNPSPSCSLELKEKLETSHLKFDASLRRLQELGKDSFLNMDNKFETYQKRLSIDYREILHCLDNLGLICAHLAAEVCLEKISDTKEESETYKECSMVCKEFLEDILSTIGVYLPQDDKSLVDLQQNHLSAVISGHVSPKLKELFHLLDSFRGDKQKQCLILVERIITAKVIERFVKKEASLAYLNVLYLTENNPSTNVSAQKMQIEIPDLFQHGKVNLLFITDVVEEGFQVPDCSCMVCFDLPKTMCSYSQSQKHAKQSNSKSIMFLERGNPKQRDHLHDLMRREVLIQDPEAPNLKSCPPPVKNGHGVKEIGSMVIPDSNITVSEEAASTQTMSDPPSRNEQLPPCKKLRLDNNLLQSNGKEKVASSKSKSSSSAAGSKKRKELHGTTCANALSGTWGENIDGATFQAYKFDFCCNISGEVYSSFSLLLESTLAEDVGKVEMDLYLVRKLVKASVSPCGQIRLSQEELVKAKYFQQFFFNGMFGKLFVGSKSQGTKREFLLQTDTSSLWHPAFMFLLLPVETNDLASSATIDWSAINSCASIVEFLKKNSLLDLRDSDGNQCNTSSGQEVLLDDKMEETNLIHFANASSDKNSLEELVVIAIHTGRIYSIVEAVSDSSAMSPFEVDASSGYATYAEYFNKKYGIVLAHPNQPLMKLKQSHHAHNLLVDFNEEMVVKTEPKAGNVRKRKPNIHAHLPPELLARIDVPRAVLKSIYLLPSVMHRLESLMLASQLREEIDCSIDNFSISSTSILEAVTTLTCPESFSMERLELLGDSVLKYVASCHLFLKYPDKDEGQLSRQRQSIISNSNLHRLTTSRKLQGYIRNGAFEPRRWTAPGQFSLFPVPCKCGIDTREVPLDPKFFTENMTIKIGKSCDMGHRWVVSKSVSDCAEALIGAYYVSGGLSASLHMMKWLGIDVDFDPNLVVEAINRVSLRCYIPKEDELIELERKIQHEFSAKFLLKEAITHSSLRESYSYERLEFLGDSVLDFLITRHLFNTYEQTGPGEMTDLRSACVNNENFAQVAVKNNLHTHLQRCATVLETQINDYLMSFQKPDETGRSIPSIQGPKALGDVVESIAGALLIDTRLDLDQVWRVFEPLLSPLVTPDKLQLPPYRELNELCDSLGYFFRVKCSNDGVKAQATIQLQLDDVLLTGDGSEQTNKLALGKAASHLLTQLEKRNISRKTSLGDNQSSMDVNLACNHSDRETLTSETTEIQSIVIPFIGPINMKKGGPRGTLHEFCKKHLWPMPTFDTSEEKSRTPFEFIDGGEKRTSFSSFTSTITLRIPNREAVMYAGEARPDKKSSFDSAVVELLYELERRKIVIIQK

Target ORF information:

RefSeq Version	NM_001161190.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana dicer-like 3 (DCL3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161190.2

ORF Insert Sequence:

ATGCATTCGT CGTTGGAGCC GGAGAAAATG GAGGAAGGTG GGGGAAGCAA TTCGCTTAAG 
AGAAAATTCT CTGAAATCGA TGGAGATCAA AATCTTGATT CTGTCTCTTC TCCTATGATG 
ACTGACTCTA ATGGTAGTTA TGAATTGAAA GTGTACGAGG TTGCTAAGAA CAGGAACATA 
ATTGCTGTTT TGGGGACAGG GATTGATAAG TCAGAGATCA CTAAGAGGCT TATCAAAGCT 
ATGGGTTCTT CTGATACAGA CAAAAGATTG ATAATTTTCT TGGCCCCAAC TGTGAATCTT 
GTTAAACAGC AATGCTGTGA GATCAGAGCA CTTGTGAATT TGAAAGTTGA AGAGTACTTT 
GGAGCTAAAG GAGTTGATAA ATGGACATCT CAGCGCTGGG ATGAGGAATT TAGCAAGCAC 
GATGTTTTAG TTATGACTCC TCAAATATTA TTGGATGTCC TTAGAAGTGC ATTCCTGAAA 
CTAGAGATGG TATGTCTTCT AATAATAGAT GAATGCCACC ATACCACTGG CAATCATCCC 
TATGCGAAGT TAATGAAGGA ATTCTATCAC GAATCCACTA GCAAACCGAA GATATTTGGA 
TTGACTGCGT CAGCCGTCAT TAGAAAAGGC ATTGTTTCCT CTCCCTCTAA CTATGCAGCT 
CAAGTATCAG AACTTGAGAG ACTCATGGAC TCAAAGATTT TTAATCCTGA AGAGCGTGAA 
GGAGTGGAAA AGTTTGCTAC AACGGTTAAA GAAGGTCCAA TATTGTATAA CCCATCACCA 
TCCTGTAGTT TGGAATTGAA AGAAAAGTTA GAAACTTCAC ACCTCAAGTT TGATGCTTCT 
CTTAGAAGGC TTCAAGAGTT GGGAAAAGAC AGTTTTCTGA ATATGGATAA TAAGTTTGAG 
ACATATCAAA AGAGATTGTC TATCGACTAC AGAGAGATTT TGCATTGCCT TGATAATCTT 
GGCCTGATTT GCGCACACTT GGCGGCTGAA GTCTGCTTGG AGAAAATCTC AGATACGAAA 
GAGGAAAGTG AAACTTATAA AGAATGCTCA ATGGTGTGCA AGGAATTTCT TGAGGATATT 
TTATCCACCA TTGGGGTGTA TTTGCCGCAA GATGATAAGA GTCTGGTAGA TTTGCAGCAA 
AACCATCTGT CAGCAGTAAT TTCTGGGCAT GTATCTCCAA AGCTAAAAGA ACTCTTCCAT 
CTATTGGATT CCTTTAGAGG TGACAAGCAA AAGCAGTGCC TTATTTTAGT TGAGAGAATT 
ATAACTGCGA AAGTGATCGA AAGATTCGTT AAGAAAGAAG CCTCTTTGGC TTACCTTAAT 
GTCTTGTATT TAACCGAAAA CAACCCCTCC ACCAATGTAT CGGCACAGAA AATGCAAATT 
GAAATCCCTG ATTTATTTCA ACATGGCAAG GTGAATCTTT TATTCATCAC AGATGTGGTT 
GAAGAGGGAT TTCAGGTTCC AGATTGCTCA TGCATGGTTT GTTTTGACCT GCCCAAAACA 
ATGTGTAGTT ACTCGCAGTC TCAAAAACAT GCCAAACAGA GTAATTCTAA GTCTATCATG 
TTTCTTGAAA GAGGGAACCC GAAGCAAAGA GACCATCTGC ATGACCTTAT GCGAAGAGAA 
GTCCTAATTC AAGATCCAGA AGCTCCAAAC TTGAAATCGT GTCCACCTCC AGTGAAAAAT 
GGACACGGTG TGAAGGAGAT TGGATCCATG GTTATCCCAG ATTCTAACAT AACTGTATCT 
GAGGAAGCAG CTTCCACACA AACTATGAGT GATCCTCCTA GCAGAAATGA GCAGTTACCA 
CCGTGTAAAA AGTTACGCTT GGATAACAAT CTCTTACAAT CCAACGGCAA AGAGAAGGTT 
GCCTCTTCTA AAAGTAAATC ATCTTCATCG GCTGCAGGTT CAAAAAAACG TAAGGAGTTG 
CACGGAACAA CCTGTGCAAA CGCATTGTCA GGAACCTGGG GAGAAAATAT TGATGGCGCC 
ACCTTTCAGG CTTATAAGTT TGACTTCTGT TGTAATATTT CTGGCGAAGT ATACTCGAGT 
TTCTCTCTTT TGCTTGAGTC AACTCTCGCC GAGGATGTTG GTAAAGTTGA GATGGACCTT 
TACTTGGTCA GGAAGCTTGT CAAGGCTTCT GTCTCACCTT GTGGCCAGAT ACGTTTGAGT 
CAAGAGGAGC TGGTCAAAGC AAAATATTTT CAGCAGTTTT TCTTTAATGG CATGTTTGGA 
AAGTTGTTTG TTGGATCTAA GTCACAGGGA ACAAAGAGAG AATTTTTGCT TCAAACTGAC 
ACTAGTTCTC TTTGGCACCC TGCCTTTATG TTTCTACTGC TACCAGTTGA AACAAATGAT 
CTAGCTTCGA GTGCGACAAT TGATTGGTCA GCTATCAACT CCTGTGCCTC AATAGTTGAG 
TTCTTGAAGA AAAATTCTCT TCTTGATCTT CGGGATAGTG ATGGGAATCA GTGCAATACC 
TCATCCGGTC AGGAAGTCTT ACTAGACGAT AAAATGGAAG AAACGAATCT GATTCATTTT 
GCCAATGCTT CGTCTGATAA AAATAGTCTC GAAGAACTTG TGGTCATTGC AATTCATACT 
GGACGGATAT ACTCTATAGT TGAAGCCGTA AGCGATTCTT CTGCTATGAG CCCCTTTGAG 
GTGGATGCCT CATCAGGCTA TGCTACTTAT GCAGAATATT TTAACAAAAA GTATGGGATT 
GTTTTAGCGC ACCCGAACCA GCCGTTGATG AAGTTGAAGC AGAGTCACCA TGCGCACAAC 
CTTTTAGTCG ACTTCAATGA AGAGATGGTT GTGAAGACAG AACCAAAAGC TGGCAATGTT 
AGGAAAAGAA AACCGAATAT CCATGCGCAT TTGCCTCCAG AGCTTTTGGC TAGAATTGAT 
GTACCGCGTG CTGTGCTAAA ATCAATCTAC TTGCTGCCTT CAGTGATGCA CCGCCTAGAG 
TCTCTAATGT TGGCCAGCCA GCTTAGGGAA GAGATTGATT GTAGCATAGA TAACTTCAGT 
ATATCAAGTA CATCGATTCT TGAAGCAGTT ACAACACTTA CATGCCCCGA ATCATTTTCA 
ATGGAGCGGT TGGAACTGCT CGGGGATTCA GTCTTGAAGT ATGTTGCGAG CTGTCATCTA 
TTCCTTAAGT ATCCTGACAA AGATGAGGGG CAACTATCAC GGCAGAGACA ATCGATTATA 
TCTAACTCAA ATCTTCACCG CTTGACAACC AGTCGCAAAC TACAGGGATA CATAAGAAAT 
GGCGCTTTTG AACCGCGTCG CTGGACTGCA CCTGGTCAAT TTTCTCTTTT TCCTGTTCCT 
TGCAAGTGTG GGATTGATAC TAGAGAAGTA CCATTGGACC CAAAATTCTT CACAGAAAAC 
ATGACTATCA AAATAGGCAA GTCTTGCGAC ATGGGTCATA GATGGGTAGT TTCAAAATCT 
GTATCAGATT GCGCTGAGGC CCTGATTGGT GCCTATTATG TAAGCGGTGG ATTGTCTGCT 
TCTCTCCATA TGATGAAATG GCTCGGTATT GACGTCGATT TTGACCCAAA CCTAGTCGTT 
GAAGCCATCA ATAGAGTTTC TCTACGGTGT TACATTCCTA AAGAAGATGA GCTCATAGAG 
TTGGAGAGAA AGATCCAACA TGAATTCTCT GCAAAGTTTC TTTTAAAAGA GGCTATCACA 
CACTCCTCTC TTCGTGAATC CTATTCATAC GAGAGATTAG AGTTTCTTGG CGATTCTGTA 
CTGGATTTTC TAATAACCCG TCATCTTTTT AACACCTACG AACAAACTGG GCCTGGAGAG 
ATGACCGATC TTCGTTCTGC ATGTGTAAAC AATGAAAATT TTGCGCAAGT TGCAGTGAAA 
AATAACCTGC ATACCCACCT TCAACGCTGT GCTACGGTTC TCGAGACTCA AATAAACGAC 
TATCTGATGT CCTTTCAAAA GCCAGATGAG ACTGGTAGAT CAATCCCTTC AATACAGGGC 
CCTAAGGCTC TTGGAGATGT TGTGGAGAGT ATCGCTGGAG CATTGCTGAT CGATACGAGG 
TTAGATCTCG ATCAAGTGTG GAGAGTCTTT GAGCCGTTGC TTTCTCCACT TGTAACTCCA 
GATAAACTTC AGCTTCCTCC ATACCGGGAG CTCAATGAGC TATGCGACTC TCTTGGGTAT 
TTCTTTCGAG TGAAATGTTC AAATGATGGT GTCAAAGCAC AAGCCACGAT CCAGTTGCAG 
CTGGATGATG TTCTTTTAAC TGGAGATGGA TCTGAACAGA CAAATAAACT GGCCTTGGGA 
AAAGCAGCTT CACATCTGCT TACACAACTT GAGAAGAGAA ACATTTCACG TAAAACCTCG 
CTCGGGGATA ATCAAAGTTC CATGGATGTC AATCTTGCTT GCAATCATAG CGACAGAGAA 
ACTCTGACTT CAGAGACTAC TGAAATCCAG AGTATAGTGA TTCCATTTAT TGGACCTATA 
AACATGAAGA AAGGCGGGCC TCGTGGAACT CTACATGAGT TTTGCAAGAA GCATCTGTGG 
CCAATGCCTA CTTTCGATAC CTCGGAAGAG AAATCCAGAA CTCCGTTTGA ATTCATAGAT 
GGCGGTGAGA AGCGGACTAG CTTCAGCAGT TTCACATCGA CCATAACCCT AAGGATACCC 
AATCGTGAGG CTGTGATGTA TGCTGGAGAA GCAAGGCCTG ACAAGAAGAG TTCCTTCGAC 
TCTGCAGTCG TGGAATTGCT TTATGAGCTC GAGCGCCGCA AGATCGTCAT AATACAAAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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DCL3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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