TIC cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIC
Entrez Gene ID	821807
Full Name	time for coffee
Synonyms	TIME FOR COFFEE
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_113136.4	NP_566705.1	time for coffee
NM_001338582.1	NP_001326717.1	time for coffee
NM_001125204.1	NP_001118676.1	time for coffee

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	TIC	NP_001118676.1
Oryza sativa (rice)	Os07g0571100	NP_001060049.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a loss of TIC function causes a major reprogramming of gene expression that predicts numerous developmental, metabolic, and stress-related phenotypes
Title: TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana.

TIC acts as an output component of the circadian oscillator to influence jasmonic acid signaling directly.
Title: TIME FOR COFFEE represses accumulation of the MYC2 transcription factor to provide time-of-day regulation of jasmonate signaling in Arabidopsis.

the regulation of iron homeostasis in plants is a major output of the TIC- and central oscillator-dependent signaling pathways.
Title: Regulation of iron homeostasis in Arabidopsis thaliana by the clock regulator time for coffee.

TIC encodes a nucleus-acting clock regulator working close to the central oscillator.
Title: TIME FOR COFFEE encodes a nuclear regulator in the Arabidopsis thaliana circadian clock.

The following TIC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb12122	NM_001125204.1 Latest version!	Arabidopsis thaliana time for coffee (TIC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAb12123	NM_113136.4 Latest version!	Arabidopsis thaliana time for coffee (TIC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAb37675	NM_001338582.1 Latest version!	Arabidopsis thaliana time for coffee (TIC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIC ( NM_001125204.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001118676.1 Arabidopsis thaliana time for coffee (TIC), mRNA.

Price & Availability↑

CloneID	OAb12122
Clone ID Related Accession (Same CDS sequence)	NM_001125204.1
Accession Version	NM_001125204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	time for coffee
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGGATAGAA ATAGAGAAGC TAGAAGAGTA CCTATGGCGG CTGCCGGAAA TGGTCTTTCC 
CGGCGGAGAC ATAGAGCCGG AAGTTTCAGA GATTCACCAG AGGAGGAAGG TCCGGTGGAG 
TTACCGGAGG CGGCGAGGTT GAGAGATCGA GGAGGGAGTA ATAAGAAGGA TCGAGATCGG 
GAACGTGATA GAGACAGAGA GAGGGAAAGA GAAAGAGATA GGGAGAGAGA TCGGTTAAAT 
AGCCGGAGTA AACGGCGGAG AGGAGAACGC TTAATGATGG TTCACGGTAA TCTAGACGAC 
GGTGGCGACG ATAGCTCGGA AGAGAGTGTC AACGACGATG AAGAATATGA CGACGGAGGT 
GTAGGACCAC CTTCTTCGTT GAAGATGCTT CCTCCGACGA GCAATAATAT CTCAGCTGCA 
TCGTTTTCAT CGTCTCTATC AAATCATCAC AACGGAGGTA GCGGTAATCT CCATCACCAC 
CATCACAGCC ACAACAATAA CCATCAGAGG AAGAATAATT TTCCGCCGAC GAAAGTTTTC 
AGATCGTCTC CTTCTCCGGC GCCGGTATCA CCTCTTGTCT CGACGTGGAA AGCCGCCGAC 
GAGATGATCG GTGTTTCGGT TCCTAGAAAA GCTCGGTCAG CGTGTACGAA GAGACCTCAT 
GAATCATGGG CGTCAAGTAC TACCGGAGGT GGTGTTTTTG CTTCCGGTGA ACAAATCCAT 
CGGCAAATAT CATCAACGTC TCCAGCTAAT AGAGTTAGTC CGGCTTCGAT TTTAGCATCT 
CCGTCTCCTC CTGCTCCTAC GTCTCCTTCT TCATCAAGCA TTTCCGTCAG GAAAAAATTG 
CCGTCGGGGA CGAAACAGAA GCCGTTACCG CCGAAGTCAT CATCGAGTAA GTTATCATCG 
CCAGTGGCTG TACAAGACGA GATCGAGATC GAGATTGCAG AAGTGTTGTA TGGTATGATG 
AGGATGCCTT CCACATCAAA ACAAGAAGCA GCCGGTAACG ATTTGACGGA GGCAGCGAAA 
TCAACTGTAG AAGTCAAATC TCGAGTCTCT TCACCGATCT CAAATCCTCA GACTCTTCCT 
CAATCATCTA TCACACTAGC TGCAAATTCT AGCTCCTCTA ATGTTTCAGC CATCGCTCCT 
AAGAGAAAGA AGCCGAGACA TGTCAAGTAC GAAGACGATA ACAGTTCACG CGTCACTACA 
ATCAAATCCG AAGCCGAAGC TCCTTCAAAA AGTCAAGTTC CGTTCAGTAA TCAACTAAAG 
AGCTCAGGAT CCGGCGAAGG AAACAGCTCC GTATTGGATT CTATCATACC ACTGACGAGA 
GAAAGCAACG CTTCGTTAGA TTCGGAGAAA AAAGAGAACA ATCTGTCAAA AGACGAAACA 
ATATTGCCGA AAGTGGAATC TTCTTCAGGA TTTAGATCCG ATGGTGAAGG AGCTAAATCG 
AGTTCGCCGG AAAAAGAGAA GTTTGAGATA GATCTGATGG CGCCACCGCC GGTGAGATCG 
TCGTCGGAGA GAGGCGGAGA GATGATGGAA TGTGTGGCGG CAGAAGCTAA ACCTAAAGTC 
ACGGAAGTGG AAACGGAAGC AAAGCCTCTG CTTAAGGAAG ATAGAAGTGA TCCGGCGATT 
CATGATTCTC AGGAGAAGAA GAGACCTAGA ATGGTTGCTG AAGCTGAACA CCATAAGTTT 
GAGAGGAATT GTGAACTCAA ACTCGACTTG GATAAATCTG ATCATGTGGG TTTAGTAAAC 
AAGCATCATG TTCAGAAACC GCCACCGCAA CAGCAACTAT CTGTTCCTGA TAAAACTGCT 
CAAGCTAGCC ATTTGCCTCT GCATATGTCT ATGCCTGGCT GGCCTGGGGG CCTTCCTACA 
ATGGGATACA TGGCACCTAC ACAAGGAGTT GTGCCTACGG ATACCAGTTC CTTATCAGCT 
GCAGCAATGC AGCCTCCACC TCATTTACTT TTTAATCAAC CAAGGCCGAA GCGATGTGCT 
ACACACTGCT ACATTGCTCG GAATATTCAA TCTCATCAGC AATTCACAAA GATGAATCCT 
TTCTGGCCTG CAGCAGCAGG CTCAGCTCCA ATGTATGGGA CCAAGGCCTG CAATCTCAGT 
CTTATGCCTC CCACAGAACT TCAGGGGAGT GTTTTGGGAA GAAGTTCCAA CCCTGTTCAA 
GATAAGAATT CTCAATCTAC GTCCAAAAGC TCAGAGACAG CTCAAAGAAA TCAATTAATG 
CTTCAACAAG CTTTGCCTCC AGGAGCAGCT AATAGCATCT TGCATGGTCC GACATTCATA 
TTCCCCTTGG GCCAACAGCC TCACGCTGCC GCTACAATAG CTGCCGCATC TGTCAGACCT 
CCTAATAGTG GCATCACATC TTCTGGACCA ACAGCTACTT CTACCTCCAT GAATGGTTCT 
GCATCAGCTA CTCCAGCTGG TGCCCCAACA ATGAGCTTCA GTTATCCTGC CATGCCGGGA 
AATGAAACGC AGTACCTGGC CATTTTGCAG AATAACGGCT ATCCATTTCC AGTTCCAGCA 
CATGTTGGTG CACAACCTGC TTATAGAGGT GCTCCTGGGC AGCCGATGCC ATTTTTTAAT 
GGTTCATTCT ATTCCTCCCA AATGATCCAG CCTCCACATC ATCAGCCACA AAAGCAGCAT 
CAGCAGCAGC TAACTGGTCA AATGCTCCAG AGCCATGCTC CTAACAACCA GAATGGGAGT 
GCTTCCACTG GTTCCTCAGC AGCTCAAAAG CATCTGCAGA ACCAACAGTT GAGGCCACCG 
ATTAATCATG GGAACTCTCA AGGATTTCCA ACTCATAAAG TGCAATCGCA GCCTTTGAAT 
TTTCAGCAGA GGCAGCAACC CAGAGAAAAT GCTACTCAAC ATAGTGAGAC TGTAGGGGAA 
GATAGCCCAT CAACTGCTGA TAGCCGGGGT TCTCGTTCCA ATGTTGCCTA TGGCCAGAAC 
TATGGTATGC AAATGCAACC AACTAACTTG GGTTTGATGA GTTCCCCTGC CCCTGGTGGT 
GGGGTGGTTG GATCTTCGAG CAGTCATGGT GAAAAGAAAT CACAGCAGCA GGTTTCAAAG 
GCTGGTGTAG AATCCTTCCA GTCACCGGGT TATGCTATGA CTTTTGCGAC GTTCAATGGG 
GCTAACACTG CTCCTACCCT AAACATGTCA TCAATTGCTC AGAATCACGC TATGTTCCAC 
AGCATGCCCG AAGCAGCAAG GCAAGGTTAT CAGATGATGG CTGCTCAAGC AGCTCAACAA 
AAAATGAACT ACGGTGCTTC ACTAGAAGAT GGAAAATCTG GGTCTATTGG TGGTGCTGCT 
ACTGCTAATA ATACCCCTGA GGAACAAAGG AAGTCAGGAG GAGGGGCAAT AGGGAAAACG 
AGTGGTGGGA ATGGTGGGCA GTCTATTGCT TTCTCTAACA AACAGGATTT AGCTGATGCA 
TCTGTTTCTG CTGTCACGAG TGGTAGCATC GTTGATAGCT CATCCCGTTT ACTCAACCTT 
GGTTCTGCTC TGCCACAGAG TTCGGGTTCT TTACCCACGT CTCATCATCA ACAACTACTA 
CAACAACAGC AGCAACAGCA TATGCAACGG AGTCAATCGC AGCAACCTTA TACCACCATG 
TACCTCCAGA AGCAGCAACG GTATGCAACT TCAGTGGCTG CTTCTGCTGC CAGAACCAAA 
GGGCCCGTGG TGAGTAACGG GAGTGGTTTC CCTGATCACA ATATGACCAC ATCACCTGCG 
GGAACCACCA AGTTTGCAAA TGCTAATTCA GGGTTCCCTC AAAACCTTGT CCAGTCGAGT 
AGCAATCAGG TTCAGTCTCA GCAGTGGAAA AACAATTCAC CCAGGACAAC AAATACTACC 
CAAGCTCAGT CTCCTTCGAT GTTGTCGCCA TCAACATCAG TTGCTGCTGC ATCTTCGCTA 
AGAAACATCC CGCATAAACA GCAAAGCCGT CCCCAGCAAT CGCAGATATC TTTTGCAGCA 
AACTCAAAAC CAATGACTTC TGGTTCACCA ATGCAGCAGG TACAAGGAGG AACCAACCAC 
CAGGCTCCGT CTCCACCAAT GTTAGTTGGG TCGCCTTCGA CGTCGTCTGT CTCCAAAAAT 
GCCAGCGGAA GCCCAAGAAC AACTGCTTCT GCTTCCTCGG CGGCAAACAA AGGTGGACAA 
GCTTCCACAA CTACTCACTC AGCATCTCAG CCGTCCAAGA ACTTACAGCC TGCATCTGCG 
GCGTCATCTG CTGGTGGAAG GAATAATGGT CCTTCTGTTC TCGGAAACCC GACCACGAGC 
TCTGGATCCA AGTCGCAACA ACAGCAGCAG TTGCCAAAGC ATGGGCTGCA GCCGCAAGCA 
CAGCTATTCT TCTCTAATCC TTATATGCAA GCTCAACATC AACATCAACA GCAACAGATC 
ACCATATCTC CTTCTGGTGG GTATTACATC CAAAGACACC AGCAACAGTC AGGTTCAGCA 
CCTGCTGTTC CAGTCACGGG AGCTGTCACC GCCACTTCAG ATCCAGCGAA AGCCATAGCG 
GCTGCATCTG CAGCGAATAA CATGAAAGGA GGTGGTGGTA TGGGTAAAAC TCAACAACAT 
CAGCTTGGGC CTCCAGGGTT CACTAATGTT CACGCTGTTT CCTCTGCGGT TCAGGTCAAA 
CCCGTGGATC AGAAGCAACA GGCGGCTTGT GCTGGCGAGA CGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001118676.1
CDS	277..4944
Translation	MDRNREARRVPMAAAGNGLSRRRHRAGSFRDSPEEEGPVELPEAARLRDRGGSNKKDRDRERDRDRERERERDRERDRLNSRSKRRRGERLMMVHGNLDDGGDDSSEESVNDDEEYDDGGVGPPSSLKMLPPTSNNISAASFSSSLSNHHNGGSGNLHHHHHSHNNNHQRKNNFPPTKVFRSSPSPAPVSPLVSTWKAADEMIGVSVPRKARSACTKRPHESWASSTTGGGVFASGEQIHRQISSTSPANRVSPASILASPSPPAPTSPSSSSISVRKKLPSGTKQKPLPPKSSSSKLSSPVAVQDEIEIEIAEVLYGMMRMPSTSKQEAAGNDLTEAAKSTVEVKSRVSSPISNPQTLPQSSITLAANSSSSNVSAIAPKRKKPRHVKYEDDNSSRVTTIKSEAEAPSKSQVPFSNQLKSSGSGEGNSSVLDSIIPLTRESNASLDSEKKENNLSKDETILPKVESSSGFRSDGEGAKSSSPEKEKFEIDLMAPPPVRSSSERGGEMMECVAAEAKPKVTEVETEAKPLLKEDRSDPAIHDSQEKKRPRMVAEAEHHKFERNCELKLDLDKSDHVGLVNKHHVQKPPPQQQLSVPDKTAQASHLPLHMSMPGWPGGLPTMGYMAPTQGVVPTDTSSLSAAAMQPPPHLLFNQPRPKRCATHCYIARNIQSHQQFTKMNPFWPAAAGSAPMYGTKACNLSLMPPTELQGSVLGRSSNPVQDKNSQSTSKSSETAQRNQLMLQQALPPGAANSILHGPTFIFPLGQQPHAAATIAAASVRPPNSGITSSGPTATSTSMNGSASATPAGAPTMSFSYPAMPGNETQYLAILQNNGYPFPVPAHVGAQPAYRGAPGQPMPFFNGSFYSSQMIQPPHHQPQKQHQQQLTGQMLQSHAPNNQNGSASTGSSAAQKHLQNQQLRPPINHGNSQGFPTHKVQSQPLNFQQRQQPRENATQHSETVGEDSPSTADSRGSRSNVAYGQNYGMQMQPTNLGLMSSPAPGGGVVGSSSSHGEKKSQQQVSKAGVESFQSPGYAMTFATFNGANTAPTLNMSSIAQNHAMFHSMPEAARQGYQMMAAQAAQQKMNYGASLEDGKSGSIGGAATANNTPEEQRKSGGGAIGKTSGGNGGQSIAFSNKQDLADASVSAVTSGSIVDSSSRLLNLGSALPQSSGSLPTSHHQQLLQQQQQQHMQRSQSQQPYTTMYLQKQQRYATSVAASAARTKGPVVSNGSGFPDHNMTTSPAGTTKFANANSGFPQNLVQSSSNQVQSQQWKNNSPRTTNTTQAQSPSMLSPSTSVAAASSLRNIPHKQQSRPQQSQISFAANSKPMTSGSPMQQVQGGTNHQAPSPPMLVGSPSTSSVSKNASGSPRTTASASSAANKGGQASTTTHSASQPSKNLQPASAASSAGGRNNGPSVLGNPTTSSGSKSQQQQQLPKHGLQPQAQLFFSNPYMQAQHQHQQQQITISPSGGYYIQRHQQQSGSAPAVPVTGAVTATSDPAKAIAAASAANNMKGGGGMGKTQQHQLGPPGFTNVHAVSSAVQVKPVDQKQQAACAGETK

Target ORF information:

RefSeq Version	NM_001125204.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana time for coffee (TIC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001125204.1

ORF Insert Sequence:

ATGGATAGAA ATAGAGAAGC TAGAAGAGTA CCTATGGCGG CTGCCGGAAA TGGTCTTTCC 
CGGCGGAGAC ATAGAGCCGG AAGTTTCAGA GATTCACCAG AGGAGGAAGG TCCGGTGGAG 
TTACCGGAGG CGGCGAGGTT GAGAGATCGA GGAGGGAGTA ATAAGAAGGA TCGAGATCGG 
GAACGTGATA GAGACAGAGA GAGGGAAAGA GAAAGAGATA GGGAGAGAGA TCGGTTAAAT 
AGCCGGAGTA AACGGCGGAG AGGAGAACGC TTAATGATGG TTCACGGTAA TCTAGACGAC 
GGTGGCGACG ATAGCTCGGA AGAGAGTGTC AACGACGATG AAGAATATGA CGACGGAGGT 
GTAGGACCAC CTTCTTCGTT GAAGATGCTT CCTCCGACGA GCAATAATAT CTCAGCTGCA 
TCGTTTTCAT CGTCTCTATC AAATCATCAC AACGGAGGTA GCGGTAATCT CCATCACCAC 
CATCACAGCC ACAACAATAA CCATCAGAGG AAGAATAATT TTCCGCCGAC GAAAGTTTTC 
AGATCGTCTC CTTCTCCGGC GCCGGTATCA CCTCTTGTCT CGACGTGGAA AGCCGCCGAC 
GAGATGATCG GTGTTTCGGT TCCTAGAAAA GCTCGGTCAG CGTGTACGAA GAGACCTCAT 
GAATCATGGG CGTCAAGTAC TACCGGAGGT GGTGTTTTTG CTTCCGGTGA ACAAATCCAT 
CGGCAAATAT CATCAACGTC TCCAGCTAAT AGAGTTAGTC CGGCTTCGAT TTTAGCATCT 
CCGTCTCCTC CTGCTCCTAC GTCTCCTTCT TCATCAAGCA TTTCCGTCAG GAAAAAATTG 
CCGTCGGGGA CGAAACAGAA GCCGTTACCG CCGAAGTCAT CATCGAGTAA GTTATCATCG 
CCAGTGGCTG TACAAGACGA GATCGAGATC GAGATTGCAG AAGTGTTGTA TGGTATGATG 
AGGATGCCTT CCACATCAAA ACAAGAAGCA GCCGGTAACG ATTTGACGGA GGCAGCGAAA 
TCAACTGTAG AAGTCAAATC TCGAGTCTCT TCACCGATCT CAAATCCTCA GACTCTTCCT 
CAATCATCTA TCACACTAGC TGCAAATTCT AGCTCCTCTA ATGTTTCAGC CATCGCTCCT 
AAGAGAAAGA AGCCGAGACA TGTCAAGTAC GAAGACGATA ACAGTTCACG CGTCACTACA 
ATCAAATCCG AAGCCGAAGC TCCTTCAAAA AGTCAAGTTC CGTTCAGTAA TCAACTAAAG 
AGCTCAGGAT CCGGCGAAGG AAACAGCTCC GTATTGGATT CTATCATACC ACTGACGAGA 
GAAAGCAACG CTTCGTTAGA TTCGGAGAAA AAAGAGAACA ATCTGTCAAA AGACGAAACA 
ATATTGCCGA AAGTGGAATC TTCTTCAGGA TTTAGATCCG ATGGTGAAGG AGCTAAATCG 
AGTTCGCCGG AAAAAGAGAA GTTTGAGATA GATCTGATGG CGCCACCGCC GGTGAGATCG 
TCGTCGGAGA GAGGCGGAGA GATGATGGAA TGTGTGGCGG CAGAAGCTAA ACCTAAAGTC 
ACGGAAGTGG AAACGGAAGC AAAGCCTCTG CTTAAGGAAG ATAGAAGTGA TCCGGCGATT 
CATGATTCTC AGGAGAAGAA GAGACCTAGA ATGGTTGCTG AAGCTGAACA CCATAAGTTT 
GAGAGGAATT GTGAACTCAA ACTCGACTTG GATAAATCTG ATCATGTGGG TTTAGTAAAC 
AAGCATCATG TTCAGAAACC GCCACCGCAA CAGCAACTAT CTGTTCCTGA TAAAACTGCT 
CAAGCTAGCC ATTTGCCTCT GCATATGTCT ATGCCTGGCT GGCCTGGGGG CCTTCCTACA 
ATGGGATACA TGGCACCTAC ACAAGGAGTT GTGCCTACGG ATACCAGTTC CTTATCAGCT 
GCAGCAATGC AGCCTCCACC TCATTTACTT TTTAATCAAC CAAGGCCGAA GCGATGTGCT 
ACACACTGCT ACATTGCTCG GAATATTCAA TCTCATCAGC AATTCACAAA GATGAATCCT 
TTCTGGCCTG CAGCAGCAGG CTCAGCTCCA ATGTATGGGA CCAAGGCCTG CAATCTCAGT 
CTTATGCCTC CCACAGAACT TCAGGGGAGT GTTTTGGGAA GAAGTTCCAA CCCTGTTCAA 
GATAAGAATT CTCAATCTAC GTCCAAAAGC TCAGAGACAG CTCAAAGAAA TCAATTAATG 
CTTCAACAAG CTTTGCCTCC AGGAGCAGCT AATAGCATCT TGCATGGTCC GACATTCATA 
TTCCCCTTGG GCCAACAGCC TCACGCTGCC GCTACAATAG CTGCCGCATC TGTCAGACCT 
CCTAATAGTG GCATCACATC TTCTGGACCA ACAGCTACTT CTACCTCCAT GAATGGTTCT 
GCATCAGCTA CTCCAGCTGG TGCCCCAACA ATGAGCTTCA GTTATCCTGC CATGCCGGGA 
AATGAAACGC AGTACCTGGC CATTTTGCAG AATAACGGCT ATCCATTTCC AGTTCCAGCA 
CATGTTGGTG CACAACCTGC TTATAGAGGT GCTCCTGGGC AGCCGATGCC ATTTTTTAAT 
GGTTCATTCT ATTCCTCCCA AATGATCCAG CCTCCACATC ATCAGCCACA AAAGCAGCAT 
CAGCAGCAGC TAACTGGTCA AATGCTCCAG AGCCATGCTC CTAACAACCA GAATGGGAGT 
GCTTCCACTG GTTCCTCAGC AGCTCAAAAG CATCTGCAGA ACCAACAGTT GAGGCCACCG 
ATTAATCATG GGAACTCTCA AGGATTTCCA ACTCATAAAG TGCAATCGCA GCCTTTGAAT 
TTTCAGCAGA GGCAGCAACC CAGAGAAAAT GCTACTCAAC ATAGTGAGAC TGTAGGGGAA 
GATAGCCCAT CAACTGCTGA TAGCCGGGGT TCTCGTTCCA ATGTTGCCTA TGGCCAGAAC 
TATGGTATGC AAATGCAACC AACTAACTTG GGTTTGATGA GTTCCCCTGC CCCTGGTGGT 
GGGGTGGTTG GATCTTCGAG CAGTCATGGT GAAAAGAAAT CACAGCAGCA GGTTTCAAAG 
GCTGGTGTAG AATCCTTCCA GTCACCGGGT TATGCTATGA CTTTTGCGAC GTTCAATGGG 
GCTAACACTG CTCCTACCCT AAACATGTCA TCAATTGCTC AGAATCACGC TATGTTCCAC 
AGCATGCCCG AAGCAGCAAG GCAAGGTTAT CAGATGATGG CTGCTCAAGC AGCTCAACAA 
AAAATGAACT ACGGTGCTTC ACTAGAAGAT GGAAAATCTG GGTCTATTGG TGGTGCTGCT 
ACTGCTAATA ATACCCCTGA GGAACAAAGG AAGTCAGGAG GAGGGGCAAT AGGGAAAACG 
AGTGGTGGGA ATGGTGGGCA GTCTATTGCT TTCTCTAACA AACAGGATTT AGCTGATGCA 
TCTGTTTCTG CTGTCACGAG TGGTAGCATC GTTGATAGCT CATCCCGTTT ACTCAACCTT 
GGTTCTGCTC TGCCACAGAG TTCGGGTTCT TTACCCACGT CTCATCATCA ACAACTACTA 
CAACAACAGC AGCAACAGCA TATGCAACGG AGTCAATCGC AGCAACCTTA TACCACCATG 
TACCTCCAGA AGCAGCAACG GTATGCAACT TCAGTGGCTG CTTCTGCTGC CAGAACCAAA 
GGGCCCGTGG TGAGTAACGG GAGTGGTTTC CCTGATCACA ATATGACCAC ATCACCTGCG 
GGAACCACCA AGTTTGCAAA TGCTAATTCA GGGTTCCCTC AAAACCTTGT CCAGTCGAGT 
AGCAATCAGG TTCAGTCTCA GCAGTGGAAA AACAATTCAC CCAGGACAAC AAATACTACC 
CAAGCTCAGT CTCCTTCGAT GTTGTCGCCA TCAACATCAG TTGCTGCTGC ATCTTCGCTA 
AGAAACATCC CGCATAAACA GCAAAGCCGT CCCCAGCAAT CGCAGATATC TTTTGCAGCA 
AACTCAAAAC CAATGACTTC TGGTTCACCA ATGCAGCAGG TACAAGGAGG AACCAACCAC 
CAGGCTCCGT CTCCACCAAT GTTAGTTGGG TCGCCTTCGA CGTCGTCTGT CTCCAAAAAT 
GCCAGCGGAA GCCCAAGAAC AACTGCTTCT GCTTCCTCGG CGGCAAACAA AGGTGGACAA 
GCTTCCACAA CTACTCACTC AGCATCTCAG CCGTCCAAGA ACTTACAGCC TGCATCTGCG 
GCGTCATCTG CTGGTGGAAG GAATAATGGT CCTTCTGTTC TCGGAAACCC GACCACGAGC 
TCTGGATCCA AGTCGCAACA ACAGCAGCAG TTGCCAAAGC ATGGGCTGCA GCCGCAAGCA 
CAGCTATTCT TCTCTAATCC TTATATGCAA GCTCAACATC AACATCAACA GCAACAGATC 
ACCATATCTC CTTCTGGTGG GTATTACATC CAAAGACACC AGCAACAGTC AGGTTCAGCA 
CCTGCTGTTC CAGTCACGGG AGCTGTCACC GCCACTTCAG ATCCAGCGAA AGCCATAGCG 
GCTGCATCTG CAGCGAATAA CATGAAAGGA GGTGGTGGTA TGGGTAAAAC TCAACAACAT 
CAGCTTGGGC CTCCAGGGTT CACTAATGTT CACGCTGTTT CCTCTGCGGT TCAGGTCAAA 
CCCGTGGATC AGAAGCAACA GGCGGCTTGT GCTGGCGAGA CGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIC ( NM_113136.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_566705.1 Arabidopsis thaliana time for coffee (TIC), mRNA.

Price & Availability↑

CloneID	OAb12123
Clone ID Related Accession (Same CDS sequence)	NM_113136.4
Accession Version	NM_113136.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	time for coffee
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_113136.3.

ATGGATAGAA ATAGAGAAGC TAGAAGAGTA CCTATGGCGG CTGCCGGAAA TGGTCTTTCC 
CGGCGGAGAC ATAGAGCCGG AAGTTTCAGA GATTCACCAG AGGAGGAAGG TCCGGTGGAG 
TTACCGGAGG CGGCGAGGTT GAGAGATCGA GGAGGGAGTA ATAAGAAGGA TCGAGATCGG 
GAACGTGATA GAGACAGAGA GAGGGAAAGA GAAAGAGATA GGGAGAGAGA TCGGTTAAAT 
AGCCGGAGTA AACGGCGGAG AGGAGAACGC TTAATGATGG TTCACGGTAA TCTAGACGAC 
GGTGGCGACG ATAGCTCGGA AGAGAGTGTC AACGACGATG AAGAATATGA CGACGGAGGT 
GTAGGACCAC CTTCTTCGTT GAAGATGCTT CCTCCGACGA GCAATAATAT CTCAGCTGCA 
TCGTTTTCAT CGTCTCTATC AAATCATCAC AACGGAGGTA GCGGTAATCT CCATCACCAC 
CATCACAGCC ACAACAATAA CCATCAGAGG AAGAATAATT TTCCGCCGAC GAAAGTTTTC 
AGATCGTCTC CTTCTCCGGC GCCGGTATCA CCTCTTGTCT CGACGTGGAA AGCCGCCGAC 
GAGATGATCG GTGTTTCGGT TCCTAGAAAA GCTCGGTCAG CGTGTACGAA GAGACCTCAT 
GAATCATGGG CGTCAAGTAC TACCGGAGGT GGTGTTTTTG CTTCCGGTGA ACAAATCCAT 
CGGCAAATAT CATCAACGTC TCCAGCTAAT AGAGTTAGTC CGGCTTCGAT TTTAGCATCT 
CCGTCTCCTC CTGCTCCTAC GTCTCCTTCT TCATCAAGCA TTTCCGTCAG GAAAAAATTG 
CCGTCGGGGA CGAAACAGAA GCCGTTACCG CCGAAGTCAT CATCGAGTAA GTTATCATCG 
CCAGTGGCTG TACAAGACGA GATCGAGATC GAGATTGCAG AAGTGTTGTA TGGTATGATG 
AGGATGCCTT CCACATCAAA ACAAGAAGCA GCCGGTAACG ATTTGACGGA GGCAGCGAAA 
TCAACTGTAG AAGTCAAATC TCGAGTCTCT TCACCGATCT CAAATCCTCA GACTCTTCCT 
CAATCATCTA TCACACTAGC TGCAAATTCT AGCTCCTCTA ATGTTTCAGC CATCGCTCCT 
AAGAGAAAGA AGCCGAGACA TGTCAAGTAC GAAGACGATA ACAGTTCACG CGTCACTACA 
ATCAAATCCG AAGCCGAAGC TCCTTCAAAA AGTCAAGTTC CGTTCAGTAA TCAACTAAAG 
AGCTCAGGAT CCGGCGAAGG AAACAGCTCC GTATTGGATT CTATCATACC ACTGACGAGA 
GAAAGCAACG CTTCGTTAGA TTCGGAGAAA AAAGAGAACA ATCTGTCAAA AGACGAAACA 
ATATTGCCGA AAGTGGAATC TTCTTCAGGA TTTAGATCCG ATGGTGAAGG AGCTAAATCG 
AGTTCGCCGG AAAAAGAGAA GTTTGAGATA GATCTGATGG CGCCACCGCC GGTGAGATCG 
TCGTCGGAGA GAGGCGGAGA GATGATGGAA TGTGTGGCGG CAGAAGCTAA ACCTAAAGTC 
ACGGAAGTGG AAACGGAAGC AAAGCCTCTG CTTAAGGAAG ATAGAAGTGA TCCGGCGATT 
CATGATTCTC AGGAGAAGAA GAGACCTAGA ATGGTTGCTG AAGCTGAACA CCATAAGTTT 
GAGAGGAATT GTGAACTCAA ACTCGACTTG GATAAATCTG ATCATGTGGG TTTAGTAAAC 
AAGCATCATG TTCAGAAACC GCCACCGCAA CAGCAACTAT CTGTTCCTGA TAAAACTGCT 
CAAGCTAGCC ATTTGCCTCT GCATATGTCT ATGCCTGGCT GGCCTGGGGG CCTTCCTACA 
ATGGGATACA TGGCACCTAC ACAAGGAGTT GTGCCTACGG ATACCAGTTC CTTATCAGCT 
GCAGCAATGC AGCCTCCACC TCATTTACTT TTTAATCAAC CAAGGCCGAA GCGATGTGCT 
ACACACTGCT ACATTGCTCG GAATATTCAA TCTCATCAGC AATTCACAAA GATGAATCCT 
TTCTGGCCTG CAGCAGCAGG CTCAGCTCCA ATGTATGGGA CCAAGGCCTG CAATCTCAGT 
CTTATGCCTC CCACAGAACT TCAGGGGAGT GTTTTGGGAA GAAGTTCCAA CCCTGTTCAA 
GATAAGAATT CTCAATCTAC GTCCAAAAGC TCAGAGACAG CTCAAAGAAA TCAATTAATG 
CTTCAACAAG CTTTGCCTCC AGGAGCAGCT AATAGCATCT TGCATGGTCC GACATTCATA 
TTCCCCTTGG GCCAACAGCC TCACGCTGCC GCTACAATAG CTGCCGCATC TGTCAGACCT 
CCTAATAGTG GCATCACATC TTCTGGACCA ACAGCTACTT CTACCTCCAT GAATGGTTCT 
GCATCAGCTA CTCCAGCTGG TGCCCCAACA ATGAGCTTCA GTTATCCTGC CATGCCGGGA 
AATGAAACGC AGTACCTGGC CATTTTGCAG AATAACGGCT ATCCATTTCC AGTTCCAGCA 
CATGTTGGTG CACAACCTGC TTATAGAGGT GCTCCTGGGC AGCCGATGCC ATTTTTTAAT 
GGTTCATTCT ATTCCTCCCA AATGATCCAG CCTCCACATC ATCAGCCACA AAAGCAGCAT 
CAGCAGCAGC TAACTGGTCA AATGCTCCAG AGCCATGCTC CTAACAACCA GAATGGGAGT 
GCTTCCACTG GTTCCTCAGC AGCTCAAAAG CATCTGCAGA ACCAACAGTT GAGGCCACCG 
ATTAATCATG GGAACTCTCA AGGATTTCCA ACTCATAAAG TGCAATCGCA GCCTTTGAAT 
TTTCAGCAGA GGCAGCAACC CAGAGAAAAT GCTACTCAAC ATAGTGAGAC TGTAGGGGAA 
GATAGCCCAT CAACTGCTGA TAGCCGGGGT TCTCGTTCCA ATGTTGCCTA TGGCCAGAAC 
TATGGTATGC AAATGCAACC AACTAACTTG GGTTTGATGA GTTCCCCTGC CCCTGGTGGT 
GGGGTGGTTG GATCTTCGAG CAGTCATGGT GAAAAGAAAT CACAGCAGCA GGTTTCAAAG 
GCTGGTGTAG AATCCTTCCA GTCACCGGGT TATGCTATGA CTTTTGCGAC GTTCAATGGG 
GCTAACACTG CTCCTACCCT AAACATGTCA TCAATTGCTC AGAATCACGC TATGTTCCAC 
AGCATGCCCG AAGCAGCAAG GCAAGGTTAT CAGATGATGG CTGCTCAAGC AGCTCAACAA 
AAAATGAACT ACGGTGCTTC ACTAGAAGAT GGAAAATCTG GGTCTATTGG TGGTGCTGCT 
ACTGCTAATA ATACCCCTGA GGAACAAAGG AAGTCAGGAG GAGGGGCAAT AGGGAAAACG 
AGTGGTGGGA ATGGTGGGCA GTCTATTGCT TTCTCTAACA AACAGGATTT AGCTGATGCA 
TCTGTTTCTG CTGTCACGAG TGGTAGCATC GTTGATAGCT CATCCCGTTT ACTCAACCTT 
GGTTCTGCTC TGCCACAGAG TTCGGGTTCT TTACCCACGT CTCATCATCA ACAACTACTA 
CAACAACAGC AGCAACAGCA TATGCAACGG AGTCAATCGC AGCAACCTTA TACCACCATG 
TACCTCCAGA AGCAGCAACG GTATGCAACT TCAGTGGCTG CTTCTGCTGC CAGAACCAAA 
GGGCCCGTGG TGAGTAACGG GAGTGGTTTC CCTGATCACA ATATGACCAC ATCACCTGCG 
GGAACCACCA AGTTTGCAAA TGCTAATTCA GGGTTCCCTC AAAACCTTGT CCAGTCGAGT 
AGCAATCAGG TTCAGTCTCA GCAGTGGAAA AACAATTCAC CCAGGACAAC AAATACTACC 
CAAGCTCAGT CTCCTTCGAT GTTGTCGCCA TCAACATCAG TTGCTGCTGC ATCTTCGCTA 
AGAAACATCC CGCATAAACA GCAAAGCCGT CCCCAGCAAT CGCAGATATC TTTTGCAGCA 
AACTCAAAAC CAATGACTTC TGGTTCACCA ATGCAGCAGG TACAAGGAGG AACCAACCAC 
CAGGCTCCGT CTCCACCAAT GTTAGTTGGG TCGCCTTCGA CGTCGTCTGT CTCCAAAAAT 
GCCAGCGGAA GCCCAAGAAC AACTGCTTCT GCTTCCTCGG CGGCAAACAA AGGTGGACAA 
GCTTCCACAA CTACTCACTC AGCATCTCAG CCGTCCAAGA ACTTACAGCC TGCATCTGCG 
GCGTCATCTG CTGGTGGAAG GAATAATGGT CCTTCTGTTC TCGGAAACCC GACCACGAGC 
TCTGGATCCA AGTCGCAACA ACAGCAGCAG TTGCCAAAGC ATGGGCTGCA GCCGCAAGCA 
CAGCTATTCT TCTCTAATCC TTATATGCAA GCTCAACATC AACATCAACA GCAACAGATC 
ACCATATCTC CTTCTGGTGG GTATTACATC CAAAGACACC AGCAACAGTC AGGTTCAGCA 
CCTGCTGTTC CAGTCACGGG AGCTGTCACC GCCACTTCAG ATCCAGCGAA AGCCATAGCG 
GCTGCATCTG CAGCGAATAA CATGAAAGGA GGTGGTGGTA TGGGTAAAAC TCAACAACAT 
CAGCTTGGGC CTCCAGGGTT CACTAATGTT CACGCTGTTT CCTCTGCGGT TCAGGTCAAA 
CCCGTGGATC AGAAGCAACA GGCGGGTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_566705.1
CDS	277..4929
Translation	MDRNREARRVPMAAAGNGLSRRRHRAGSFRDSPEEEGPVELPEAARLRDRGGSNKKDRDRERDRDRERERERDRERDRLNSRSKRRRGERLMMVHGNLDDGGDDSSEESVNDDEEYDDGGVGPPSSLKMLPPTSNNISAASFSSSLSNHHNGGSGNLHHHHHSHNNNHQRKNNFPPTKVFRSSPSPAPVSPLVSTWKAADEMIGVSVPRKARSACTKRPHESWASSTTGGGVFASGEQIHRQISSTSPANRVSPASILASPSPPAPTSPSSSSISVRKKLPSGTKQKPLPPKSSSSKLSSPVAVQDEIEIEIAEVLYGMMRMPSTSKQEAAGNDLTEAAKSTVEVKSRVSSPISNPQTLPQSSITLAANSSSSNVSAIAPKRKKPRHVKYEDDNSSRVTTIKSEAEAPSKSQVPFSNQLKSSGSGEGNSSVLDSIIPLTRESNASLDSEKKENNLSKDETILPKVESSSGFRSDGEGAKSSSPEKEKFEIDLMAPPPVRSSSERGGEMMECVAAEAKPKVTEVETEAKPLLKEDRSDPAIHDSQEKKRPRMVAEAEHHKFERNCELKLDLDKSDHVGLVNKHHVQKPPPQQQLSVPDKTAQASHLPLHMSMPGWPGGLPTMGYMAPTQGVVPTDTSSLSAAAMQPPPHLLFNQPRPKRCATHCYIARNIQSHQQFTKMNPFWPAAAGSAPMYGTKACNLSLMPPTELQGSVLGRSSNPVQDKNSQSTSKSSETAQRNQLMLQQALPPGAANSILHGPTFIFPLGQQPHAAATIAAASVRPPNSGITSSGPTATSTSMNGSASATPAGAPTMSFSYPAMPGNETQYLAILQNNGYPFPVPAHVGAQPAYRGAPGQPMPFFNGSFYSSQMIQPPHHQPQKQHQQQLTGQMLQSHAPNNQNGSASTGSSAAQKHLQNQQLRPPINHGNSQGFPTHKVQSQPLNFQQRQQPRENATQHSETVGEDSPSTADSRGSRSNVAYGQNYGMQMQPTNLGLMSSPAPGGGVVGSSSSHGEKKSQQQVSKAGVESFQSPGYAMTFATFNGANTAPTLNMSSIAQNHAMFHSMPEAARQGYQMMAAQAAQQKMNYGASLEDGKSGSIGGAATANNTPEEQRKSGGGAIGKTSGGNGGQSIAFSNKQDLADASVSAVTSGSIVDSSSRLLNLGSALPQSSGSLPTSHHQQLLQQQQQQHMQRSQSQQPYTTMYLQKQQRYATSVAASAARTKGPVVSNGSGFPDHNMTTSPAGTTKFANANSGFPQNLVQSSSNQVQSQQWKNNSPRTTNTTQAQSPSMLSPSTSVAAASSLRNIPHKQQSRPQQSQISFAANSKPMTSGSPMQQVQGGTNHQAPSPPMLVGSPSTSSVSKNASGSPRTTASASSAANKGGQASTTTHSASQPSKNLQPASAASSAGGRNNGPSVLGNPTTSSGSKSQQQQQLPKHGLQPQAQLFFSNPYMQAQHQHQQQQITISPSGGYYIQRHQQQSGSAPAVPVTGAVTATSDPAKAIAAASAANNMKGGGGMGKTQQHQLGPPGFTNVHAVSSAVQVKPVDQKQQAGE

Target ORF information:

RefSeq Version	NM_113136.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana time for coffee (TIC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_113136.4

ORF Insert Sequence:

ATGGATAGAA ATAGAGAAGC TAGAAGAGTA CCTATGGCGG CTGCCGGAAA TGGTCTTTCC 
CGGCGGAGAC ATAGAGCCGG AAGTTTCAGA GATTCACCAG AGGAGGAAGG TCCGGTGGAG 
TTACCGGAGG CGGCGAGGTT GAGAGATCGA GGAGGGAGTA ATAAGAAGGA TCGAGATCGG 
GAACGTGATA GAGACAGAGA GAGGGAAAGA GAAAGAGATA GGGAGAGAGA TCGGTTAAAT 
AGCCGGAGTA AACGGCGGAG AGGAGAACGC TTAATGATGG TTCACGGTAA TCTAGACGAC 
GGTGGCGACG ATAGCTCGGA AGAGAGTGTC AACGACGATG AAGAATATGA CGACGGAGGT 
GTAGGACCAC CTTCTTCGTT GAAGATGCTT CCTCCGACGA GCAATAATAT CTCAGCTGCA 
TCGTTTTCAT CGTCTCTATC AAATCATCAC AACGGAGGTA GCGGTAATCT CCATCACCAC 
CATCACAGCC ACAACAATAA CCATCAGAGG AAGAATAATT TTCCGCCGAC GAAAGTTTTC 
AGATCGTCTC CTTCTCCGGC GCCGGTATCA CCTCTTGTCT CGACGTGGAA AGCCGCCGAC 
GAGATGATCG GTGTTTCGGT TCCTAGAAAA GCTCGGTCAG CGTGTACGAA GAGACCTCAT 
GAATCATGGG CGTCAAGTAC TACCGGAGGT GGTGTTTTTG CTTCCGGTGA ACAAATCCAT 
CGGCAAATAT CATCAACGTC TCCAGCTAAT AGAGTTAGTC CGGCTTCGAT TTTAGCATCT 
CCGTCTCCTC CTGCTCCTAC GTCTCCTTCT TCATCAAGCA TTTCCGTCAG GAAAAAATTG 
CCGTCGGGGA CGAAACAGAA GCCGTTACCG CCGAAGTCAT CATCGAGTAA GTTATCATCG 
CCAGTGGCTG TACAAGACGA GATCGAGATC GAGATTGCAG AAGTGTTGTA TGGTATGATG 
AGGATGCCTT CCACATCAAA ACAAGAAGCA GCCGGTAACG ATTTGACGGA GGCAGCGAAA 
TCAACTGTAG AAGTCAAATC TCGAGTCTCT TCACCGATCT CAAATCCTCA GACTCTTCCT 
CAATCATCTA TCACACTAGC TGCAAATTCT AGCTCCTCTA ATGTTTCAGC CATCGCTCCT 
AAGAGAAAGA AGCCGAGACA TGTCAAGTAC GAAGACGATA ACAGTTCACG CGTCACTACA 
ATCAAATCCG AAGCCGAAGC TCCTTCAAAA AGTCAAGTTC CGTTCAGTAA TCAACTAAAG 
AGCTCAGGAT CCGGCGAAGG AAACAGCTCC GTATTGGATT CTATCATACC ACTGACGAGA 
GAAAGCAACG CTTCGTTAGA TTCGGAGAAA AAAGAGAACA ATCTGTCAAA AGACGAAACA 
ATATTGCCGA AAGTGGAATC TTCTTCAGGA TTTAGATCCG ATGGTGAAGG AGCTAAATCG 
AGTTCGCCGG AAAAAGAGAA GTTTGAGATA GATCTGATGG CGCCACCGCC GGTGAGATCG 
TCGTCGGAGA GAGGCGGAGA GATGATGGAA TGTGTGGCGG CAGAAGCTAA ACCTAAAGTC 
ACGGAAGTGG AAACGGAAGC AAAGCCTCTG CTTAAGGAAG ATAGAAGTGA TCCGGCGATT 
CATGATTCTC AGGAGAAGAA GAGACCTAGA ATGGTTGCTG AAGCTGAACA CCATAAGTTT 
GAGAGGAATT GTGAACTCAA ACTCGACTTG GATAAATCTG ATCATGTGGG TTTAGTAAAC 
AAGCATCATG TTCAGAAACC GCCACCGCAA CAGCAACTAT CTGTTCCTGA TAAAACTGCT 
CAAGCTAGCC ATTTGCCTCT GCATATGTCT ATGCCTGGCT GGCCTGGGGG CCTTCCTACA 
ATGGGATACA TGGCACCTAC ACAAGGAGTT GTGCCTACGG ATACCAGTTC CTTATCAGCT 
GCAGCAATGC AGCCTCCACC TCATTTACTT TTTAATCAAC CAAGGCCGAA GCGATGTGCT 
ACACACTGCT ACATTGCTCG GAATATTCAA TCTCATCAGC AATTCACAAA GATGAATCCT 
TTCTGGCCTG CAGCAGCAGG CTCAGCTCCA ATGTATGGGA CCAAGGCCTG CAATCTCAGT 
CTTATGCCTC CCACAGAACT TCAGGGGAGT GTTTTGGGAA GAAGTTCCAA CCCTGTTCAA 
GATAAGAATT CTCAATCTAC GTCCAAAAGC TCAGAGACAG CTCAAAGAAA TCAATTAATG 
CTTCAACAAG CTTTGCCTCC AGGAGCAGCT AATAGCATCT TGCATGGTCC GACATTCATA 
TTCCCCTTGG GCCAACAGCC TCACGCTGCC GCTACAATAG CTGCCGCATC TGTCAGACCT 
CCTAATAGTG GCATCACATC TTCTGGACCA ACAGCTACTT CTACCTCCAT GAATGGTTCT 
GCATCAGCTA CTCCAGCTGG TGCCCCAACA ATGAGCTTCA GTTATCCTGC CATGCCGGGA 
AATGAAACGC AGTACCTGGC CATTTTGCAG AATAACGGCT ATCCATTTCC AGTTCCAGCA 
CATGTTGGTG CACAACCTGC TTATAGAGGT GCTCCTGGGC AGCCGATGCC ATTTTTTAAT 
GGTTCATTCT ATTCCTCCCA AATGATCCAG CCTCCACATC ATCAGCCACA AAAGCAGCAT 
CAGCAGCAGC TAACTGGTCA AATGCTCCAG AGCCATGCTC CTAACAACCA GAATGGGAGT 
GCTTCCACTG GTTCCTCAGC AGCTCAAAAG CATCTGCAGA ACCAACAGTT GAGGCCACCG 
ATTAATCATG GGAACTCTCA AGGATTTCCA ACTCATAAAG TGCAATCGCA GCCTTTGAAT 
TTTCAGCAGA GGCAGCAACC CAGAGAAAAT GCTACTCAAC ATAGTGAGAC TGTAGGGGAA 
GATAGCCCAT CAACTGCTGA TAGCCGGGGT TCTCGTTCCA ATGTTGCCTA TGGCCAGAAC 
TATGGTATGC AAATGCAACC AACTAACTTG GGTTTGATGA GTTCCCCTGC CCCTGGTGGT 
GGGGTGGTTG GATCTTCGAG CAGTCATGGT GAAAAGAAAT CACAGCAGCA GGTTTCAAAG 
GCTGGTGTAG AATCCTTCCA GTCACCGGGT TATGCTATGA CTTTTGCGAC GTTCAATGGG 
GCTAACACTG CTCCTACCCT AAACATGTCA TCAATTGCTC AGAATCACGC TATGTTCCAC 
AGCATGCCCG AAGCAGCAAG GCAAGGTTAT CAGATGATGG CTGCTCAAGC AGCTCAACAA 
AAAATGAACT ACGGTGCTTC ACTAGAAGAT GGAAAATCTG GGTCTATTGG TGGTGCTGCT 
ACTGCTAATA ATACCCCTGA GGAACAAAGG AAGTCAGGAG GAGGGGCAAT AGGGAAAACG 
AGTGGTGGGA ATGGTGGGCA GTCTATTGCT TTCTCTAACA AACAGGATTT AGCTGATGCA 
TCTGTTTCTG CTGTCACGAG TGGTAGCATC GTTGATAGCT CATCCCGTTT ACTCAACCTT 
GGTTCTGCTC TGCCACAGAG TTCGGGTTCT TTACCCACGT CTCATCATCA ACAACTACTA 
CAACAACAGC AGCAACAGCA TATGCAACGG AGTCAATCGC AGCAACCTTA TACCACCATG 
TACCTCCAGA AGCAGCAACG GTATGCAACT TCAGTGGCTG CTTCTGCTGC CAGAACCAAA 
GGGCCCGTGG TGAGTAACGG GAGTGGTTTC CCTGATCACA ATATGACCAC ATCACCTGCG 
GGAACCACCA AGTTTGCAAA TGCTAATTCA GGGTTCCCTC AAAACCTTGT CCAGTCGAGT 
AGCAATCAGG TTCAGTCTCA GCAGTGGAAA AACAATTCAC CCAGGACAAC AAATACTACC 
CAAGCTCAGT CTCCTTCGAT GTTGTCGCCA TCAACATCAG TTGCTGCTGC ATCTTCGCTA 
AGAAACATCC CGCATAAACA GCAAAGCCGT CCCCAGCAAT CGCAGATATC TTTTGCAGCA 
AACTCAAAAC CAATGACTTC TGGTTCACCA ATGCAGCAGG TACAAGGAGG AACCAACCAC 
CAGGCTCCGT CTCCACCAAT GTTAGTTGGG TCGCCTTCGA CGTCGTCTGT CTCCAAAAAT 
GCCAGCGGAA GCCCAAGAAC AACTGCTTCT GCTTCCTCGG CGGCAAACAA AGGTGGACAA 
GCTTCCACAA CTACTCACTC AGCATCTCAG CCGTCCAAGA ACTTACAGCC TGCATCTGCG 
GCGTCATCTG CTGGTGGAAG GAATAATGGT CCTTCTGTTC TCGGAAACCC GACCACGAGC 
TCTGGATCCA AGTCGCAACA ACAGCAGCAG TTGCCAAAGC ATGGGCTGCA GCCGCAAGCA 
CAGCTATTCT TCTCTAATCC TTATATGCAA GCTCAACATC AACATCAACA GCAACAGATC 
ACCATATCTC CTTCTGGTGG GTATTACATC CAAAGACACC AGCAACAGTC AGGTTCAGCA 
CCTGCTGTTC CAGTCACGGG AGCTGTCACC GCCACTTCAG ATCCAGCGAA AGCCATAGCG 
GCTGCATCTG CAGCGAATAA CATGAAAGGA GGTGGTGGTA TGGGTAAAAC TCAACAACAT 
CAGCTTGGGC CTCCAGGGTT CACTAATGTT CACGCTGTTT CCTCTGCGGT TCAGGTCAAA 
CCCGTGGATC AGAAGCAACA GGCGGGTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIC ( NM_001338582.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001326717.1 Arabidopsis thaliana time for coffee (TIC), mRNA.

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CloneID	OAb37675
Clone ID Related Accession (Same CDS sequence)	NM_001338582.1
Accession Version	NM_001338582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	time for coffee
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGGATAGAA ATAGAGAAGC TAGAAGAGTA CCTATGGCGG CTGCCGGAAA TGGTCTTTCC 
CGGCGGAGAC ATAGAGCCGG AAGTTTCAGA GATTCACCAG AGGAGGAAGG TCCGGTGGAG 
TTACCGGAGG CGGCGAGGTT GAGAGATCGA GGAGGGAGTA ATAAGAAGGA TCGAGATCGG 
GAACGTGATA GAGACAGAGA GAGGGAAAGA GAAAGAGATA GGGAGAGAGA TCGGTTAAAT 
AGCCGGAGTA AACGGCGGAG AGGAGAACGC TTAATGATGG TTCACGGTAA TCTAGACGAC 
GGTGGCGACG ATAGCTCGGA AGAGAGTGTC AACGACGATG AAGAATATGA CGACGGAGGT 
GTAGGACCAC CTTCTTCGTT GAAGATGCTT CCTCCGACGA GCAATAATAT CTCAGCTGCA 
TCGTTTTCAT CGTCTCTATC AAATCATCAC AACGGAGGTA GCGGTAATCT CCATCACCAC 
CATCACAGCC ACAACAATAA CCATCAGAGG AAGAATAATT TTCCGCCGAC GAAAGTTTTC 
AGATCGTCTC CTTCTCCGGC GCCGGTATCA CCTCTTGTCT CGACGTGGAA AGCCGCCGAC 
GAGATGATCG GTGTTTCGGT TCCTAGAAAA GCTCGGTCAG CGTGTACGAA GAGACCTCAT 
GAATCATGGG CGTCAAGTAC TACCGGAGGT GGTGTTTTTG CTTCCGGTGA ACAAATCCAT 
CGGCAAATAT CATCAACGTC TCCAGCTAAT AGAGTTAGTC CGGCTTCGAT TTTAGCATCT 
CCGTCTCCTC CTGCTCCTAC GTCTCCTTCT TCATCAAGCA TTTCCGTCAG GAAAAAATTG 
CCGTCGGGGA CGAAACAGAA GCCGTTACCG CCGAAGTCAT CATCGAGTAA GTTATCATCG 
CCAGTGGCTG TACAAGACGA GATCGAGATC GAGATTGCAG AAGTGTTGTA TGGTATGATG 
AGGATGCCTT CCACATCAAA ACAAGAAGCA GCCGGTAACG ATTTGACGGA GGCAGCGAAA 
TCAACTGTAG AAGTCAAATC TCGAGTCTCT TCACCGATCT CAAATCCTCA GACTCTTCCT 
CAATCATCTA TCACACTAGC TGCAAATTCT AGCTCCTCTA ATGTTTCAGC CATCGCTCCT 
AAGAGAAAGA AGCCGAGACA TGTCAAGTAC GAAGACGATA ACAGTTCACG CGTCACTACA 
ATCAAATCCG AAGCCGAAGC TCCTTCAAAA AGTCAAGTTC CGTTCAGTAA TCAACTAAAG 
AGCTCAGGAT CCGGCGAAGG AAACAGCTCC GTATTGGATT CTATCATACC ACTGACGAGA 
GAAAGCAACG CTTCGTTAGA TTCGGAGAAA AAAGAGAACA ATCTGTCAAA AGACGAAACA 
ATATTGCCGA AAGTGGAATC TTCTTCAGGA TTTAGATCCG ATGGTGAAGG AGCTAAATCG 
AGTTCGCCGG AAAAAGAGAA GTTTGAGATA GATCTGATGG CGCCACCGCC GGTGAGATCG 
TCGTCGGAGA GAGGCGGAGA GATGATGGAA TGTGTGGCGG CAGAAGCTAA ACCTAAAGTC 
ACGGAAGTGG AAACGCAGGA AGCAAAGCCT CTGCTTAAGG AAGATAGAAG TGATCCGGCG 
ATTCATGATT CTCAGGAGAA GAAGAGACCT AGAATGGTTG CTGAAGCTGA ACACCATAAG 
TTTGAGAGGA ATTGTGAACT CAAACTCGAC TTGGATAAAT CTGATCATGT GGGTTTAGTA 
AACAAGCATC ATGTTCAGAA ACCGCCACCG CAACAGCAAC TATCTGTTCC TGATAAAACT 
GCTCAAGCTA GCCATTTGCC TCTGCATATG TCTATGCCTG GCTGGCCTGG GGGCCTTCCT 
ACAATGGGAT ACATGGCACC TACACAAGGA GTTGTGCCTA CGGATACCAG TTCCTTATCA 
GCTGCAGCAA TGCAGCCTCC ACCTCATTTA CTTTTTAATC AACCAAGGCC GAAGCGATGT 
GCTACACACT GCTACATTGC TCGGAATATT CAATCTCATC AGCAATTCAC AAAGATGAAT 
CCTTTCTGGC CTGCAGCAGC AGGCTCAGCT CCAATGTATG GGACCAAGGC CTGCAATCTC 
AGTCTTATGC CTCCCACAGA ACTTCAGGGG AGTGTTTTGG GAAGAAGTTC CAACCCTGTT 
CAAGATAAGA ATTCTCAATC TACGTCCAAA AGCTCAGAGA CAGCTCAAAG AAATCAATTA 
ATGCTTCAAC AAGCTTTGCC TCCAGGAGCA GCTAATAGCA TCTTGCATGG TCCGACATTC 
ATATTCCCCT TGGGCCAACA GCCTCACGCT GCCGCTACAA TAGCTGCCGC ATCTGTCAGA 
CCTCCTAATA GTGGCATCAC ATCTTCTGGA CCAACAGCTA CTTCTACCTC CATGAATGGT 
TCTGCATCAG CTACTCCAGC TGGTGCCCCA ACAATGAGCT TCAGTTATCC TGCCATGCCG 
GGAAATGAAA CGCAGTACCT GGCCATTTTG CAGAATAACG GCTATCCATT TCCAGTTCCA 
GCACATGTTG GTGCACAACC TGCTTATAGA GGTGCTCCTG GGCAGCCGAT GCCATTTTTT 
AATGGTTCAT TCTATTCCTC CCAAATGATC CAGCCTCCAC ATCATCAGCC ACAAAAGCAG 
CATCAGCAGC AGCTAACTGG TCAAATGCTC CAGAGCCATG CTCCTAACAA CCAGAATGGG 
AGTGCTTCCA CTGGTTCCTC AGCAGCTCAA AAGCATCTGC AGAACCAACA GTTGAGGCCA 
CCGATTAATC ATGGGAACTC TCAAGGATTT CCAACTCATA AAGTGCAATC GCAGCCTTTG 
AATTTTCAGC AGAGGCAGCA ACCCAGAGAA AATGCTACTC AACATAGTGA GACTGTAGGG 
GAAGATAGCC CATCAACTGC TGATAGCCGG GGTTCTCGTT CCAATGTTGC CTATGGCCAG 
AACTATGGTA TGCAAATGCA ACCAACTAAC TTGGGTTTGA TGAGTTCCCC TGCCCCTGGT 
GGTGGGGTGG TTGGATCTTC GAGCAGTCAT GGTGAAAAGA AATCACAGCA GCAGGTTTCA 
AAGGCTGGTG TAGAATCCTT CCAGTCACCG GGTTATGCTA TGACTTTTGC GACGTTCAAT 
GGGGCTAACA CTGCTCCTAC CCTAAACATG TCATCAATTG CTCAGAATCA CGCTATGTTC 
CACAGCATGC CCGAAGCAGC AAGGCAAGGT TATCAGATGA TGGCTGCTCA AGCAGCTCAA 
CAAAAAATGA ACTACGGTGC TTCACTAGAA GATGGAAAAT CTGGGTCTAT TGGTGGTGCT 
GCTACTGCTA ATAATACCCC TGAGGAACAA AGGAAGTCAG GAGGAGGGGC AATAGGGAAA 
ACGAGTGGTG GGAATGGTGG GCAGTCTATT GCTTTCTCTA ACAAACAGGA TTTAGCTGAT 
GCATCTGTTT CTGCTGTCAC GAGTGGTAGC ATCGTTGATA GCTCATCCCG TTTACTCAAC 
CTTGGTTCTG CTCTGCCACA GAGTTCGGGT TCTTTACCCA CGTCTCATCA TCAACAACTA 
CTACAACAAC AGCAGCAACA GCATATGCAA CGGAGTCAAT CGCAGCAACC TTATACCACC 
ATGTACCTCC AGAAGCAGCA ACGGTATGCA ACTTCAGTGG CTGCTTCTGC TGCCAGAACC 
AAAGGGCCCG TGGTGAGTAA CGGGAGTGGT TTCCCTGATC ACAATATGAC CACATCACCT 
GCGGGAACCA CCAAGTTTGC AAATGCTAAT TCAGGGTTCC CTCAAAACCT TGTCCAGTCG 
AGTAGCAATC AGGTTCAGTC TCAGCAGTGG AAAAACAATT CACCCAGGAC AACAAATACT 
ACCCAAGCTC AGTCTCCTTC GATGTTGTCG CCATCAACAT CAGTTGCTGC TGCATCTTCG 
CTAAGAAACA TCCCGCATAA ACAGCAAAGC CGTCCCCAGC AATCGCAGAT ATCTTTTGCA 
GCAAACTCAA AACCAATGAC TTCTGGTTCA CCAATGCAGC AGGTACAAGG AGGAACCAAC 
CACCAGGCTC CGTCTCCACC AATGTTAGTT GGGTCGCCTT CGACGTCGTC TGTCTCCAAA 
AATGCCAGCG GAAGCCCAAG AACAACTGCT TCTGCTTCCT CGGCGGCAAA CAAAGGTGGA 
CAAGCTTCCA CAACTACTCA CTCAGCATCT CAGCCGTCCA AGAACTTACA GCCTGCATCT 
GCGGCGTCAT CTGCTGGTGG AAGGAATAAT GGTCCTTCTG TTCTCGGAAA CCCGACCACG 
AGCTCTGGAT CCAAGTCGCA ACAACAGCAG CAGTTGCCAA AGCATGGGCT GCAGCCGCAA 
GCACAGCTAT TCTTCTCTAA TCCTTATATG CAAGCTCAAC ATCAACATCA ACAGCAACAG 
ATCACCATAT CTCCTTCTGG TGGGTATTAC ATCCAAAGAC ACCAGCAACA GTCAGGTTCA 
GCACCTGCTG TTCCAGTCAC GGGAGCTGTC ACCGCCACTT CAGATCCAGC GAAAGCCATA 
GCGGCTGCAT CTGCAGCGAA TAACATGAAA GGAGGTGGTG GTATGGGTAA AACTCAACAA 
CATCAGCTTG GGCCTCCAGG GTTCACTAAT GTTCACGCTG TTTCCTCTGC GGTTCAGGTC 
AAACCCGTGG ATCAGAAGCA ACAGGCGGGT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001326717.1
CDS	277..4932
Translation	MDRNREARRVPMAAAGNGLSRRRHRAGSFRDSPEEEGPVELPEAARLRDRGGSNKKDRDRERDRDRERERERDRERDRLNSRSKRRRGERLMMVHGNLDDGGDDSSEESVNDDEEYDDGGVGPPSSLKMLPPTSNNISAASFSSSLSNHHNGGSGNLHHHHHSHNNNHQRKNNFPPTKVFRSSPSPAPVSPLVSTWKAADEMIGVSVPRKARSACTKRPHESWASSTTGGGVFASGEQIHRQISSTSPANRVSPASILASPSPPAPTSPSSSSISVRKKLPSGTKQKPLPPKSSSSKLSSPVAVQDEIEIEIAEVLYGMMRMPSTSKQEAAGNDLTEAAKSTVEVKSRVSSPISNPQTLPQSSITLAANSSSSNVSAIAPKRKKPRHVKYEDDNSSRVTTIKSEAEAPSKSQVPFSNQLKSSGSGEGNSSVLDSIIPLTRESNASLDSEKKENNLSKDETILPKVESSSGFRSDGEGAKSSSPEKEKFEIDLMAPPPVRSSSERGGEMMECVAAEAKPKVTEVETQEAKPLLKEDRSDPAIHDSQEKKRPRMVAEAEHHKFERNCELKLDLDKSDHVGLVNKHHVQKPPPQQQLSVPDKTAQASHLPLHMSMPGWPGGLPTMGYMAPTQGVVPTDTSSLSAAAMQPPPHLLFNQPRPKRCATHCYIARNIQSHQQFTKMNPFWPAAAGSAPMYGTKACNLSLMPPTELQGSVLGRSSNPVQDKNSQSTSKSSETAQRNQLMLQQALPPGAANSILHGPTFIFPLGQQPHAAATIAAASVRPPNSGITSSGPTATSTSMNGSASATPAGAPTMSFSYPAMPGNETQYLAILQNNGYPFPVPAHVGAQPAYRGAPGQPMPFFNGSFYSSQMIQPPHHQPQKQHQQQLTGQMLQSHAPNNQNGSASTGSSAAQKHLQNQQLRPPINHGNSQGFPTHKVQSQPLNFQQRQQPRENATQHSETVGEDSPSTADSRGSRSNVAYGQNYGMQMQPTNLGLMSSPAPGGGVVGSSSSHGEKKSQQQVSKAGVESFQSPGYAMTFATFNGANTAPTLNMSSIAQNHAMFHSMPEAARQGYQMMAAQAAQQKMNYGASLEDGKSGSIGGAATANNTPEEQRKSGGGAIGKTSGGNGGQSIAFSNKQDLADASVSAVTSGSIVDSSSRLLNLGSALPQSSGSLPTSHHQQLLQQQQQQHMQRSQSQQPYTTMYLQKQQRYATSVAASAARTKGPVVSNGSGFPDHNMTTSPAGTTKFANANSGFPQNLVQSSSNQVQSQQWKNNSPRTTNTTQAQSPSMLSPSTSVAAASSLRNIPHKQQSRPQQSQISFAANSKPMTSGSPMQQVQGGTNHQAPSPPMLVGSPSTSSVSKNASGSPRTTASASSAANKGGQASTTTHSASQPSKNLQPASAASSAGGRNNGPSVLGNPTTSSGSKSQQQQQLPKHGLQPQAQLFFSNPYMQAQHQHQQQQITISPSGGYYIQRHQQQSGSAPAVPVTGAVTATSDPAKAIAAASAANNMKGGGGMGKTQQHQLGPPGFTNVHAVSSAVQVKPVDQKQQAGE

Target ORF information:

RefSeq Version	NM_001338582.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana time for coffee (TIC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001338582.1

ORF Insert Sequence:

ATGGATAGAA ATAGAGAAGC TAGAAGAGTA CCTATGGCGG CTGCCGGAAA TGGTCTTTCC 
CGGCGGAGAC ATAGAGCCGG AAGTTTCAGA GATTCACCAG AGGAGGAAGG TCCGGTGGAG 
TTACCGGAGG CGGCGAGGTT GAGAGATCGA GGAGGGAGTA ATAAGAAGGA TCGAGATCGG 
GAACGTGATA GAGACAGAGA GAGGGAAAGA GAAAGAGATA GGGAGAGAGA TCGGTTAAAT 
AGCCGGAGTA AACGGCGGAG AGGAGAACGC TTAATGATGG TTCACGGTAA TCTAGACGAC 
GGTGGCGACG ATAGCTCGGA AGAGAGTGTC AACGACGATG AAGAATATGA CGACGGAGGT 
GTAGGACCAC CTTCTTCGTT GAAGATGCTT CCTCCGACGA GCAATAATAT CTCAGCTGCA 
TCGTTTTCAT CGTCTCTATC AAATCATCAC AACGGAGGTA GCGGTAATCT CCATCACCAC 
CATCACAGCC ACAACAATAA CCATCAGAGG AAGAATAATT TTCCGCCGAC GAAAGTTTTC 
AGATCGTCTC CTTCTCCGGC GCCGGTATCA CCTCTTGTCT CGACGTGGAA AGCCGCCGAC 
GAGATGATCG GTGTTTCGGT TCCTAGAAAA GCTCGGTCAG CGTGTACGAA GAGACCTCAT 
GAATCATGGG CGTCAAGTAC TACCGGAGGT GGTGTTTTTG CTTCCGGTGA ACAAATCCAT 
CGGCAAATAT CATCAACGTC TCCAGCTAAT AGAGTTAGTC CGGCTTCGAT TTTAGCATCT 
CCGTCTCCTC CTGCTCCTAC GTCTCCTTCT TCATCAAGCA TTTCCGTCAG GAAAAAATTG 
CCGTCGGGGA CGAAACAGAA GCCGTTACCG CCGAAGTCAT CATCGAGTAA GTTATCATCG 
CCAGTGGCTG TACAAGACGA GATCGAGATC GAGATTGCAG AAGTGTTGTA TGGTATGATG 
AGGATGCCTT CCACATCAAA ACAAGAAGCA GCCGGTAACG ATTTGACGGA GGCAGCGAAA 
TCAACTGTAG AAGTCAAATC TCGAGTCTCT TCACCGATCT CAAATCCTCA GACTCTTCCT 
CAATCATCTA TCACACTAGC TGCAAATTCT AGCTCCTCTA ATGTTTCAGC CATCGCTCCT 
AAGAGAAAGA AGCCGAGACA TGTCAAGTAC GAAGACGATA ACAGTTCACG CGTCACTACA 
ATCAAATCCG AAGCCGAAGC TCCTTCAAAA AGTCAAGTTC CGTTCAGTAA TCAACTAAAG 
AGCTCAGGAT CCGGCGAAGG AAACAGCTCC GTATTGGATT CTATCATACC ACTGACGAGA 
GAAAGCAACG CTTCGTTAGA TTCGGAGAAA AAAGAGAACA ATCTGTCAAA AGACGAAACA 
ATATTGCCGA AAGTGGAATC TTCTTCAGGA TTTAGATCCG ATGGTGAAGG AGCTAAATCG 
AGTTCGCCGG AAAAAGAGAA GTTTGAGATA GATCTGATGG CGCCACCGCC GGTGAGATCG 
TCGTCGGAGA GAGGCGGAGA GATGATGGAA TGTGTGGCGG CAGAAGCTAA ACCTAAAGTC 
ACGGAAGTGG AAACGCAGGA AGCAAAGCCT CTGCTTAAGG AAGATAGAAG TGATCCGGCG 
ATTCATGATT CTCAGGAGAA GAAGAGACCT AGAATGGTTG CTGAAGCTGA ACACCATAAG 
TTTGAGAGGA ATTGTGAACT CAAACTCGAC TTGGATAAAT CTGATCATGT GGGTTTAGTA 
AACAAGCATC ATGTTCAGAA ACCGCCACCG CAACAGCAAC TATCTGTTCC TGATAAAACT 
GCTCAAGCTA GCCATTTGCC TCTGCATATG TCTATGCCTG GCTGGCCTGG GGGCCTTCCT 
ACAATGGGAT ACATGGCACC TACACAAGGA GTTGTGCCTA CGGATACCAG TTCCTTATCA 
GCTGCAGCAA TGCAGCCTCC ACCTCATTTA CTTTTTAATC AACCAAGGCC GAAGCGATGT 
GCTACACACT GCTACATTGC TCGGAATATT CAATCTCATC AGCAATTCAC AAAGATGAAT 
CCTTTCTGGC CTGCAGCAGC AGGCTCAGCT CCAATGTATG GGACCAAGGC CTGCAATCTC 
AGTCTTATGC CTCCCACAGA ACTTCAGGGG AGTGTTTTGG GAAGAAGTTC CAACCCTGTT 
CAAGATAAGA ATTCTCAATC TACGTCCAAA AGCTCAGAGA CAGCTCAAAG AAATCAATTA 
ATGCTTCAAC AAGCTTTGCC TCCAGGAGCA GCTAATAGCA TCTTGCATGG TCCGACATTC 
ATATTCCCCT TGGGCCAACA GCCTCACGCT GCCGCTACAA TAGCTGCCGC ATCTGTCAGA 
CCTCCTAATA GTGGCATCAC ATCTTCTGGA CCAACAGCTA CTTCTACCTC CATGAATGGT 
TCTGCATCAG CTACTCCAGC TGGTGCCCCA ACAATGAGCT TCAGTTATCC TGCCATGCCG 
GGAAATGAAA CGCAGTACCT GGCCATTTTG CAGAATAACG GCTATCCATT TCCAGTTCCA 
GCACATGTTG GTGCACAACC TGCTTATAGA GGTGCTCCTG GGCAGCCGAT GCCATTTTTT 
AATGGTTCAT TCTATTCCTC CCAAATGATC CAGCCTCCAC ATCATCAGCC ACAAAAGCAG 
CATCAGCAGC AGCTAACTGG TCAAATGCTC CAGAGCCATG CTCCTAACAA CCAGAATGGG 
AGTGCTTCCA CTGGTTCCTC AGCAGCTCAA AAGCATCTGC AGAACCAACA GTTGAGGCCA 
CCGATTAATC ATGGGAACTC TCAAGGATTT CCAACTCATA AAGTGCAATC GCAGCCTTTG 
AATTTTCAGC AGAGGCAGCA ACCCAGAGAA AATGCTACTC AACATAGTGA GACTGTAGGG 
GAAGATAGCC CATCAACTGC TGATAGCCGG GGTTCTCGTT CCAATGTTGC CTATGGCCAG 
AACTATGGTA TGCAAATGCA ACCAACTAAC TTGGGTTTGA TGAGTTCCCC TGCCCCTGGT 
GGTGGGGTGG TTGGATCTTC GAGCAGTCAT GGTGAAAAGA AATCACAGCA GCAGGTTTCA 
AAGGCTGGTG TAGAATCCTT CCAGTCACCG GGTTATGCTA TGACTTTTGC GACGTTCAAT 
GGGGCTAACA CTGCTCCTAC CCTAAACATG TCATCAATTG CTCAGAATCA CGCTATGTTC 
CACAGCATGC CCGAAGCAGC AAGGCAAGGT TATCAGATGA TGGCTGCTCA AGCAGCTCAA 
CAAAAAATGA ACTACGGTGC TTCACTAGAA GATGGAAAAT CTGGGTCTAT TGGTGGTGCT 
GCTACTGCTA ATAATACCCC TGAGGAACAA AGGAAGTCAG GAGGAGGGGC AATAGGGAAA 
ACGAGTGGTG GGAATGGTGG GCAGTCTATT GCTTTCTCTA ACAAACAGGA TTTAGCTGAT 
GCATCTGTTT CTGCTGTCAC GAGTGGTAGC ATCGTTGATA GCTCATCCCG TTTACTCAAC 
CTTGGTTCTG CTCTGCCACA GAGTTCGGGT TCTTTACCCA CGTCTCATCA TCAACAACTA 
CTACAACAAC AGCAGCAACA GCATATGCAA CGGAGTCAAT CGCAGCAACC TTATACCACC 
ATGTACCTCC AGAAGCAGCA ACGGTATGCA ACTTCAGTGG CTGCTTCTGC TGCCAGAACC 
AAAGGGCCCG TGGTGAGTAA CGGGAGTGGT TTCCCTGATC ACAATATGAC CACATCACCT 
GCGGGAACCA CCAAGTTTGC AAATGCTAAT TCAGGGTTCC CTCAAAACCT TGTCCAGTCG 
AGTAGCAATC AGGTTCAGTC TCAGCAGTGG AAAAACAATT CACCCAGGAC AACAAATACT 
ACCCAAGCTC AGTCTCCTTC GATGTTGTCG CCATCAACAT CAGTTGCTGC TGCATCTTCG 
CTAAGAAACA TCCCGCATAA ACAGCAAAGC CGTCCCCAGC AATCGCAGAT ATCTTTTGCA 
GCAAACTCAA AACCAATGAC TTCTGGTTCA CCAATGCAGC AGGTACAAGG AGGAACCAAC 
CACCAGGCTC CGTCTCCACC AATGTTAGTT GGGTCGCCTT CGACGTCGTC TGTCTCCAAA 
AATGCCAGCG GAAGCCCAAG AACAACTGCT TCTGCTTCCT CGGCGGCAAA CAAAGGTGGA 
CAAGCTTCCA CAACTACTCA CTCAGCATCT CAGCCGTCCA AGAACTTACA GCCTGCATCT 
GCGGCGTCAT CTGCTGGTGG AAGGAATAAT GGTCCTTCTG TTCTCGGAAA CCCGACCACG 
AGCTCTGGAT CCAAGTCGCA ACAACAGCAG CAGTTGCCAA AGCATGGGCT GCAGCCGCAA 
GCACAGCTAT TCTTCTCTAA TCCTTATATG CAAGCTCAAC ATCAACATCA ACAGCAACAG 
ATCACCATAT CTCCTTCTGG TGGGTATTAC ATCCAAAGAC ACCAGCAACA GTCAGGTTCA 
GCACCTGCTG TTCCAGTCAC GGGAGCTGTC ACCGCCACTT CAGATCCAGC GAAAGCCATA 
GCGGCTGCAT CTGCAGCGAA TAACATGAAA GGAGGTGGTG GTATGGGTAA AACTCAACAA 
CATCAGCTTG GGCCTCCAGG GTTCACTAAT GTTCACGCTG TTTCCTCTGC GGTTCAGGTC 
AAACCCGTGG ATCAGAAGCA ACAGGCGGGT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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TIC cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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