SPY cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPY
Entrez Gene ID	820327
Full Name	Tetratricopeptide repeat (TPR)-like superfamily protein
Synonyms	SPINDLY
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_111987.4	NP_187761.1	Tetratricopeptide repeat (TPR)-like superfamily protein
NM_202557.2	NP_974286.1	Tetratricopeptide repeat (TPR)-like superfamily protein

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os08g0559300	NP_001062501.1
Arabidopsis thaliana (thale cress)	SPY	NP_187761.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The study revealed that SPY-dependent protein O-fucosylation plays a key role in regulating plant development.
Title: The Arabidopsis O-fucosyltransferase SPINDLY activates nuclear growth repressor DELLA.

posttranslational modification of TCP14/15 by SPY inhibits their proteolysis.
Title: The Putative O-Linked N-Acetylglucosamine Transferase SPINDLY Inhibits Class I TCP Proteolysis to Promote Sensitivity to Cytokinin.

SPINDLY regulates cortex proliferation by maintaining cellular redox homeostasis
Title: SPINDLY, ERECTA, and its ligand STOMAGEN have a role in redox-mediated cortex proliferation in the Arabidopsis root.

Data suggest that responses of leaves and flowers to CK are mediated by SPY-dependent TCP14 and TCP15 activities.
Title: The Arabidopsis O-linked N-acetylglucosamine transferase SPINDLY interacts with class I TCPs to facilitate cytokinin responses in leaves and flowers.

SPY plays a negative role in plant abiotic stress tolerance, probably by integrating environmental stress signals via gibberellic acid and Cytokinins cross talk.
Title: SPINDLY, a negative regulator of gibberellic acid signaling, is involved in the plant abiotic stress response.

The following SPY gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPY cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb10888	NM_111987.4 Latest version!	Arabidopsis thaliana Tetratricopeptide repeat (TPR)-like superfamily protein (SPY), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb10889	NM_202557.2 Latest version!	Arabidopsis thaliana Tetratricopeptide repeat (TPR)-like superfamily protein (SPY), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPY ( NM_111987.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_187761.1 Arabidopsis thaliana Tetratricopeptide repeat (TPR)-like superfamily protein (SPY), mRNA.

Price & Availability↑

CloneID	OAb10888
Clone ID Related Accession (Same CDS sequence)	NM_111987.4
Accession Version	NM_111987.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Tetratricopeptide repeat (TPR)-like superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_111987.3.

ATGGTGGGAC TGGAAGATGA TACTGAGAGA GAGAGGTCAC CAGTCGTAGA GAATGGTTTT 
TCCAATGGGT CTCGGTCTTC TTCTTCTAGC GCAGGTGTTT TGTCTCCATC ACGAAAGGTC 
ACTCAGGGGA ACGATACACT TTCTTATGCC AATATTCTTC GGGCAAGAAA CAAGTTTGCT 
GATGCGCTTG CTCTCTATGA GGCTATGCTG GAGAAAGATA GCAAGAATGT TGAAGCTCAC 
ATTGGAAAAG GGATATGCTT GCAGACGCAG AACAAAGGGA ATCTAGCTTT CGATTGTTTT 
TCTGAAGCGA TCAGGTTGGA TCCGCATAAT GCTTGTGCCC TTACACACTG TGGTATACTT 
CATAAAGAAG AAGGACGCCT CGTAGAAGCT GCTGAGTCCT ACCAGAAAGC ATTGATGGCA 
GATGCATCAT ACAAGCCAGC AGCAGAGTGT TTAGCCATTG TTTTGACCGA CCTTGGAACT 
AGCCTGAAGC TGGCTGGGAA TACTCAGGAA GGAATTCAAA AGTATTACGA AGCCCTTAAG 
ATTGACCCAC ACTATGCTCC TGCATATTAC AACTTAGGTG TTGTATACTC CGAAATGATG 
CAATATGACA ATGCCTTGAG CTGCTACGAG AAGGCTGCAC TTGAGAGGCC TATGTATGCT 
GAAGCATATT GTAACATGGG TGTCATTTAT AAGAACCGTG GTGACTTGGA GATGGCAATC 
ACTTGTTATG AGAGATGTCT AGCTGTGTCT CCAAACTTTG AGATTGCGAA GAACAATATG 
GCCATAGCTC TGACAGATTT AGGAACAAAG GTTAAACTTG AAGGCGATGT AACCCAAGGA 
GTGGCATATT ACAAGAAGGC TCTCTATTAT AACTGGCACT ATGCAGATGC TATGTACAAT 
CTTGGGGTGG CTTATGGAGA AATGCTAAAG TTCGACATGG CGATTGTCTT CTATGAGCTT 
GCTTTCCACT TTAATCCACA TTGTGCTGAG GCTTGCAACA ATTTGGGAGT ACTTTACAAA 
GACCGTGACA ACCTTGATAA AGCTGTGGAG TGTTATCAGA TGGCTCTATC AATCAAACCA 
AATTTTGCAC AGTCGCTTAA TAACCTTGGT GTCGTCTATA CAGTCCAGGG GAAAATGGAT 
GCTGCTGCCA GCATGATTGA GAAGGCTATA CTTGCTAATC CCACATATGC AGAAGCTTTT 
AACAACCTAG GTGTTCTTTA CAGAGATGCT GGAAATATAA CTATGGCTAT CGATGCTTAT 
GAGGAATGCC TTAAGATAGA TCCAGATTCT CGCAATGCTG GCCAGAACCG ATTGCTTGCC 
ATGAATTACA TAAATGAAGG ACTCGATGAC AAACTATTTG AGGCTCACAG AGACTGGGGT 
TGGCGCTTCA CAAGATTACA CCCTCAATAC ACTTCATGGG ATAATCTGAA AGATCCAGAG 
CGACCTATCA CCATTGGATA TATCTCACCA GATTTCTTCA CTCATTCAGT ATCTTATTTC 
ATTGAAGCTC CCCTTACGCA TCATGATTAT ACAAAGTACA AAGTGGTGGT TTATTCAGCG 
GTAGTTAAGG CAGATGCAAA AACATACAGG TTTAGGGATA AAGTGTTGAA GAAAGGTGGA 
GTTTGGAAGG ATATATACGG GATAGATGAG AAAAAGATAG CAAGTATGGT CAGAGAGGAC 
AAAATCGACA TTTTGGTAGA ACTCACTGGT CATACGGCAA ACAACAAGTT GGGAACAATG 
GCCTGCAGAC CAGCACCGGT TCAGGTTACT TGGATCGGCT ATCCAAATAC TACGGGTTTG 
CCCACTGTTG ATTACAGAAT TACAGATTCG TTGGCTGATC CACCAGATAC CAAACAAAAG 
CAGGTCGAGG AGCTGGTTAG GCTTCCGGAC TGTTTTCTTT GTTATACACC TTCCCCAGAG 
GCTGGTCCTG TTTGTCCAAC ACCTGCGCTT TCTAATGGCT TTGTCACATT TGGTAGTTTC 
AACAACCTCG CAAAGATAAC TCCTAAGGTG CTGCAAGTGT GGGCTAGGAT ACTGTGTGCA 
GTTCCCAATT CTCGTCTAGT GGTAAAATGC AAACCTTTCT GCTGCGATAG CATTAGGCAG 
AGGTTTCTCA CCACGCTGGA GCAGCTTGGG TTAGAATCAA AGCGTGTTGA TCTCTTGCCT 
TTGATTCTTT TCAATCACGA CCATATGCAA GCCTATTCCT TGATGGATAT TAGTTTGGAC 
ACATTCCCTT ATGCTGGAAC TACCACTACC TGTGAGTCTC TCTACATGGG AGTTCCATGT 
GTTACAATGG CTGGCTCAGT ACATGCTCAT AATGTTGGTG TCAGTCTTCT CACTAAAGTT 
GGATTAGGAC ACCTGGTTGC CAAAAATGAG GATGAGTATG TTCAGCTATC TGTTGATCTA 
GCCTCTGATG TCACAGCTCT TTCTAAATTG AGAATGAGTC TCCGGGATCT AATGGCTGGA 
TCTCCTGTTT GTAATGGTCC TTCCTTTGCT GTTGGTCTTG AATCCGCATA TCGGAATATG 
TGGAAAAAGT ACTGCAAAGG TGAAGTGCCG TCCTTAAGGC GAATGGAAAT GCTGCAAAAA 
GAGGTCCATG ATGATCCCTT AATCTCAAAA GACTTGGGAC CATCAAGAGT CAGCGTTACT 
GGAGAAGCCA CTCCGTCTCT CAAGGCCAAT GGTTCTGCTC CTGTACCTTC CTCTTTACCA 
ACCCAATCCC CGCAGCTCTC AAAGAGAATG GACTCCACTA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_187761.1
CDS	636..3380
Translation	MVGLEDDTERERSPVVENGFSNGSRSSSSSAGVLSPSRKVTQGNDTLSYANILRARNKFADALALYEAMLEKDSKNVEAHIGKGICLQTQNKGNLAFDCFSEAIRLDPHNACALTHCGILHKEEGRLVEAAESYQKALMADASYKPAAECLAIVLTDLGTSLKLAGNTQEGIQKYYEALKIDPHYAPAYYNLGVVYSEMMQYDNALSCYEKAALERPMYAEAYCNMGVIYKNRGDLEMAITCYERCLAVSPNFEIAKNNMAIALTDLGTKVKLEGDVTQGVAYYKKALYYNWHYADAMYNLGVAYGEMLKFDMAIVFYELAFHFNPHCAEACNNLGVLYKDRDNLDKAVECYQMALSIKPNFAQSLNNLGVVYTVQGKMDAAASMIEKAILANPTYAEAFNNLGVLYRDAGNITMAIDAYEECLKIDPDSRNAGQNRLLAMNYINEGLDDKLFEAHRDWGWRFTRLHPQYTSWDNLKDPERPITIGYISPDFFTHSVSYFIEAPLTHHDYTKYKVVVYSAVVKADAKTYRFRDKVLKKGGVWKDIYGIDEKKIASMVREDKIDILVELTGHTANNKLGTMACRPAPVQVTWIGYPNTTGLPTVDYRITDSLADPPDTKQKQVEELVRLPDCFLCYTPSPEAGPVCPTPALSNGFVTFGSFNNLAKITPKVLQVWARILCAVPNSRLVVKCKPFCCDSIRQRFLTTLEQLGLESKRVDLLPLILFNHDHMQAYSLMDISLDTFPYAGTTTTCESLYMGVPCVTMAGSVHAHNVGVSLLTKVGLGHLVAKNEDEYVQLSVDLASDVTALSKLRMSLRDLMAGSPVCNGPSFAVGLESAYRNMWKKYCKGEVPSLRRMEMLQKEVHDDPLISKDLGPSRVSVTGEATPSLKANGSAPVPSSLPTQSPQLSKRMDSTS

Target ORF information:

RefSeq Version	NM_111987.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Tetratricopeptide repeat (TPR)-like superfamily protein (SPY), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_111987.4

ORF Insert Sequence:

ATGGTGGGAC TGGAAGATGA TACTGAGAGA GAGAGGTCAC CAGTCGTAGA GAATGGTTTT 
TCCAATGGGT CTCGGTCTTC TTCTTCTAGC GCAGGTGTTT TGTCTCCATC ACGAAAGGTC 
ACTCAGGGGA ACGATACACT TTCTTATGCC AATATTCTTC GGGCAAGAAA CAAGTTTGCT 
GATGCGCTTG CTCTCTATGA GGCTATGCTG GAGAAAGATA GCAAGAATGT TGAAGCTCAC 
ATTGGAAAAG GGATATGCTT GCAGACGCAG AACAAAGGGA ATCTAGCTTT CGATTGTTTT 
TCTGAAGCGA TCAGGTTGGA TCCGCATAAT GCTTGTGCCC TTACACACTG TGGTATACTT 
CATAAAGAAG AAGGACGCCT CGTAGAAGCT GCTGAGTCCT ACCAGAAAGC ATTGATGGCA 
GATGCATCAT ACAAGCCAGC AGCAGAGTGT TTAGCCATTG TTTTGACCGA CCTTGGAACT 
AGCCTGAAGC TGGCTGGGAA TACTCAGGAA GGAATTCAAA AGTATTACGA AGCCCTTAAG 
ATTGACCCAC ACTATGCTCC TGCATATTAC AACTTAGGTG TTGTATACTC CGAAATGATG 
CAATATGACA ATGCCTTGAG CTGCTACGAG AAGGCTGCAC TTGAGAGGCC TATGTATGCT 
GAAGCATATT GTAACATGGG TGTCATTTAT AAGAACCGTG GTGACTTGGA GATGGCAATC 
ACTTGTTATG AGAGATGTCT AGCTGTGTCT CCAAACTTTG AGATTGCGAA GAACAATATG 
GCCATAGCTC TGACAGATTT AGGAACAAAG GTTAAACTTG AAGGCGATGT AACCCAAGGA 
GTGGCATATT ACAAGAAGGC TCTCTATTAT AACTGGCACT ATGCAGATGC TATGTACAAT 
CTTGGGGTGG CTTATGGAGA AATGCTAAAG TTCGACATGG CGATTGTCTT CTATGAGCTT 
GCTTTCCACT TTAATCCACA TTGTGCTGAG GCTTGCAACA ATTTGGGAGT ACTTTACAAA 
GACCGTGACA ACCTTGATAA AGCTGTGGAG TGTTATCAGA TGGCTCTATC AATCAAACCA 
AATTTTGCAC AGTCGCTTAA TAACCTTGGT GTCGTCTATA CAGTCCAGGG GAAAATGGAT 
GCTGCTGCCA GCATGATTGA GAAGGCTATA CTTGCTAATC CCACATATGC AGAAGCTTTT 
AACAACCTAG GTGTTCTTTA CAGAGATGCT GGAAATATAA CTATGGCTAT CGATGCTTAT 
GAGGAATGCC TTAAGATAGA TCCAGATTCT CGCAATGCTG GCCAGAACCG ATTGCTTGCC 
ATGAATTACA TAAATGAAGG ACTCGATGAC AAACTATTTG AGGCTCACAG AGACTGGGGT 
TGGCGCTTCA CAAGATTACA CCCTCAATAC ACTTCATGGG ATAATCTGAA AGATCCAGAG 
CGACCTATCA CCATTGGATA TATCTCACCA GATTTCTTCA CTCATTCAGT ATCTTATTTC 
ATTGAAGCTC CCCTTACGCA TCATGATTAT ACAAAGTACA AAGTGGTGGT TTATTCAGCG 
GTAGTTAAGG CAGATGCAAA AACATACAGG TTTAGGGATA AAGTGTTGAA GAAAGGTGGA 
GTTTGGAAGG ATATATACGG GATAGATGAG AAAAAGATAG CAAGTATGGT CAGAGAGGAC 
AAAATCGACA TTTTGGTAGA ACTCACTGGT CATACGGCAA ACAACAAGTT GGGAACAATG 
GCCTGCAGAC CAGCACCGGT TCAGGTTACT TGGATCGGCT ATCCAAATAC TACGGGTTTG 
CCCACTGTTG ATTACAGAAT TACAGATTCG TTGGCTGATC CACCAGATAC CAAACAAAAG 
CAGGTCGAGG AGCTGGTTAG GCTTCCGGAC TGTTTTCTTT GTTATACACC TTCCCCAGAG 
GCTGGTCCTG TTTGTCCAAC ACCTGCGCTT TCTAATGGCT TTGTCACATT TGGTAGTTTC 
AACAACCTCG CAAAGATAAC TCCTAAGGTG CTGCAAGTGT GGGCTAGGAT ACTGTGTGCA 
GTTCCCAATT CTCGTCTAGT GGTAAAATGC AAACCTTTCT GCTGCGATAG CATTAGGCAG 
AGGTTTCTCA CCACGCTGGA GCAGCTTGGG TTAGAATCAA AGCGTGTTGA TCTCTTGCCT 
TTGATTCTTT TCAATCACGA CCATATGCAA GCCTATTCCT TGATGGATAT TAGTTTGGAC 
ACATTCCCTT ATGCTGGAAC TACCACTACC TGTGAGTCTC TCTACATGGG AGTTCCATGT 
GTTACAATGG CTGGCTCAGT ACATGCTCAT AATGTTGGTG TCAGTCTTCT CACTAAAGTT 
GGATTAGGAC ACCTGGTTGC CAAAAATGAG GATGAGTATG TTCAGCTATC TGTTGATCTA 
GCCTCTGATG TCACAGCTCT TTCTAAATTG AGAATGAGTC TCCGGGATCT AATGGCTGGA 
TCTCCTGTTT GTAATGGTCC TTCCTTTGCT GTTGGTCTTG AATCCGCATA TCGGAATATG 
TGGAAAAAGT ACTGCAAAGG TGAAGTGCCG TCCTTAAGGC GAATGGAAAT GCTGCAAAAA 
GAGGTCCATG ATGATCCCTT AATCTCAAAA GACTTGGGAC CATCAAGAGT CAGCGTTACT 
GGAGAAGCCA CTCCGTCTCT CAAGGCCAAT GGTTCTGCTC CTGTACCTTC CTCTTTACCA 
ACCCAATCCC CGCAGCTCTC AAAGAGAATG GACTCCACTA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPY ( NM_202557.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_974286.1 Arabidopsis thaliana Tetratricopeptide repeat (TPR)-like superfamily protein (SPY), mRNA.

Price & Availability↑

CloneID	OAb10889
Clone ID Related Accession (Same CDS sequence)	NM_202557.2
Accession Version	NM_202557.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Tetratricopeptide repeat (TPR)-like superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Apr 18, 2007 this sequence version replaced NM_202557.1.

ATGGTGGGAC TGGAAGATGA TACTGAGAGA GAGAGGTCAC CAGTCGTAGA GAATGGTTTT 
TCCAATGGGT CTCGGTCTTC TTCTTCTAGC GCAGGTGTTT TGTCTCCATC ACGAAAGGTC 
ACTCAGGGGA ACGATACACT TTCTTATGCC AATATTCTTC GGGCAAGAAA CAAGTTTGCT 
GATGCGCTTG CTCTCTATGA GGCTATGCTG GAGAAAGATA GCAAGAATGT TGAAGCTCAC 
ATTGGAAAAG GGATATGCTT GCAGACGCAG AACAAAGGGA ATCTAGCTTT CGATTGTTTT 
TCTGAAGCGA TCAGGTTGGA TCCGCATAAT GCTTGTGCCC TTACACACTG TGGTATACTT 
CATAAAGAAG AAGGACGCCT CGTAGAAGCT GCTGAGTCCT ACCAGAAAGC ATTGATGGCA 
GATGCATCAT ACAAGCCAGC AGCAGAGTGT TTAGCCATTG TTTTGACCGA CCTTGGAACT 
AGCCTGAAGC TGGCTGGGAA TACTCAGGAA GGAATTCAAA AGTATTACGA AGCCCTTAAG 
ATTGACCCAC ACTATGCTCC TGCATATTAC AACTTAGGTG TTGTATACTC CGAAATGATG 
CAATATGACA ATGCCTTGAG CTGCTACGAG AAGGCTGCAC TTGAGAGGCC TATGTATGCT 
GAAGCATATT GTAACATGGA TGCTGGAAAT ATAACTATGG CTATCGATGC TTATGAGGAA 
TGCCTTAAGA TAGATCCAGA TTCTCGCAAT GCTGGCCAGA ACCGATTGCT TGCCATGAAT 
TACATAAATG AAGGACTCGA TGACAAACTA TTTGAGGCTC ACAGAGACTG GGGTTGGCGC 
TTCACAAGAT TACACCCTCA ATACACTTCA TGGGATAATC TGAAAGATCC AGAGCGACCT 
ATCACCATTG GATATATCTC ACCAGATTTC TTCACTCATT CAGTATCTTA TTTCATTGAA 
GCTCCCCTTA CGCATCATGA TTATACAAAG TACAAAGTGG TGGTTTATTC AGCGGTAGTT 
AAGGCAGATG CAAAAACATA CAGGTTTAGG GATAAAGTGT TGAAGAAAGG TGGAGTTTGG 
AAGGATATAT ACGGGATAGA TGAGAAAAAG ATAGCAAGTA TGGTCAGAGA GGACAAAATC 
GACATTTTGG TAGAACTCAC TGGTCATACG GCAAACAACA AGTTGGGAAC AATGGCCTGC 
AGACCAGCAC CGGTTCAGGT TACTTGGATC GGCTATCCAA ATACTACGGG TTTGCCCACT 
GTTGATTACA GAATTACAGA TTCGTTGGCT GATCCACCAG ATACCAAACA AAAGCAGGTC 
GAGGAGCTGG TTAGGCTTCC GGACTGTTTT CTTTGTTATA CACCTTCCCC AGAGGCTGGT 
CCTGTTTGTC CAACACCTGC GCTTTCTAAT GGCTTTGTCA CATTTGGTAG TTTCAACAAC 
CTCGCAAAGA TAACTCCTAA GGTGCTGCAA GTGTGGGCTA GGATACTGTG TGCAGTTCCC 
AATTCTCGTC TAGTGGTAAA ATGCAAACCT TTCTGCTGCG ATAGCATTAG GCAGAGGTTT 
CTCACCACGC TGGAGCAGCT TGGGTTAGAA TCAAAGCGTG TTGATCTCTT GCCTTTGATT 
CTTTTCAATC ACGACCATAT GCAAGCCTAT TCCTTGATGG ATATTAGTTT GGACACATTC 
CCTTATGCTG GAACTACCAC TACCTGTGAG TCTCTCTACA TGGGAGTTCC ATGTGTTACA 
ATGGCTGGCT CAGTACATGC TCATAATGTT GGTGTCAGTC TTCTCACTAA AGTTGGATTA 
GGACACCTGG TTGCCAAAAA TGAGGATGAG TATGTTCAGC TATCTGTTGA TCTAGCCTCT 
GATGTCACAG CTCTTTCTAA ATTGAGAATG AGTCTCCGGG ATCTAATGGC TGGATCTCCT 
GTTTGTAATG GTCCTTCCTT TGCTGTTGGT CTTGAATCCG CATATCGGAA TATGTGGAAA 
AAGTACTGCA AAGGTGAAGT GCCGTCCTTA AGGCGAATGG AAATGCTGCA AAAAGAGGTC 
CATGATGATC CCTTAATCTC AAAAGACTTG GGACCATCAA GAGTCAGCGT TACTGGAGAA 
GCCACTCCGT CTCTCAAGGC CAATGGTTCT GCTCCTGTAC CTTCCTCTTT ACCAACCCAA 
TCCCCGCAGC TCTCAAAGAG AATGGACTCC ACTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_974286.1
CDS	361..2559
Translation	MVGLEDDTERERSPVVENGFSNGSRSSSSSAGVLSPSRKVTQGNDTLSYANILRARNKFADALALYEAMLEKDSKNVEAHIGKGICLQTQNKGNLAFDCFSEAIRLDPHNACALTHCGILHKEEGRLVEAAESYQKALMADASYKPAAECLAIVLTDLGTSLKLAGNTQEGIQKYYEALKIDPHYAPAYYNLGVVYSEMMQYDNALSCYEKAALERPMYAEAYCNMDAGNITMAIDAYEECLKIDPDSRNAGQNRLLAMNYINEGLDDKLFEAHRDWGWRFTRLHPQYTSWDNLKDPERPITIGYISPDFFTHSVSYFIEAPLTHHDYTKYKVVVYSAVVKADAKTYRFRDKVLKKGGVWKDIYGIDEKKIASMVREDKIDILVELTGHTANNKLGTMACRPAPVQVTWIGYPNTTGLPTVDYRITDSLADPPDTKQKQVEELVRLPDCFLCYTPSPEAGPVCPTPALSNGFVTFGSFNNLAKITPKVLQVWARILCAVPNSRLVVKCKPFCCDSIRQRFLTTLEQLGLESKRVDLLPLILFNHDHMQAYSLMDISLDTFPYAGTTTTCESLYMGVPCVTMAGSVHAHNVGVSLLTKVGLGHLVAKNEDEYVQLSVDLASDVTALSKLRMSLRDLMAGSPVCNGPSFAVGLESAYRNMWKKYCKGEVPSLRRMEMLQKEVHDDPLISKDLGPSRVSVTGEATPSLKANGSAPVPSSLPTQSPQLSKRMDSTS

Target ORF information:

RefSeq Version	NM_202557.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Tetratricopeptide repeat (TPR)-like superfamily protein (SPY), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_202557.2

ORF Insert Sequence:

ATGGTGGGAC TGGAAGATGA TACTGAGAGA GAGAGGTCAC CAGTCGTAGA GAATGGTTTT 
TCCAATGGGT CTCGGTCTTC TTCTTCTAGC GCAGGTGTTT TGTCTCCATC ACGAAAGGTC 
ACTCAGGGGA ACGATACACT TTCTTATGCC AATATTCTTC GGGCAAGAAA CAAGTTTGCT 
GATGCGCTTG CTCTCTATGA GGCTATGCTG GAGAAAGATA GCAAGAATGT TGAAGCTCAC 
ATTGGAAAAG GGATATGCTT GCAGACGCAG AACAAAGGGA ATCTAGCTTT CGATTGTTTT 
TCTGAAGCGA TCAGGTTGGA TCCGCATAAT GCTTGTGCCC TTACACACTG TGGTATACTT 
CATAAAGAAG AAGGACGCCT CGTAGAAGCT GCTGAGTCCT ACCAGAAAGC ATTGATGGCA 
GATGCATCAT ACAAGCCAGC AGCAGAGTGT TTAGCCATTG TTTTGACCGA CCTTGGAACT 
AGCCTGAAGC TGGCTGGGAA TACTCAGGAA GGAATTCAAA AGTATTACGA AGCCCTTAAG 
ATTGACCCAC ACTATGCTCC TGCATATTAC AACTTAGGTG TTGTATACTC CGAAATGATG 
CAATATGACA ATGCCTTGAG CTGCTACGAG AAGGCTGCAC TTGAGAGGCC TATGTATGCT 
GAAGCATATT GTAACATGGA TGCTGGAAAT ATAACTATGG CTATCGATGC TTATGAGGAA 
TGCCTTAAGA TAGATCCAGA TTCTCGCAAT GCTGGCCAGA ACCGATTGCT TGCCATGAAT 
TACATAAATG AAGGACTCGA TGACAAACTA TTTGAGGCTC ACAGAGACTG GGGTTGGCGC 
TTCACAAGAT TACACCCTCA ATACACTTCA TGGGATAATC TGAAAGATCC AGAGCGACCT 
ATCACCATTG GATATATCTC ACCAGATTTC TTCACTCATT CAGTATCTTA TTTCATTGAA 
GCTCCCCTTA CGCATCATGA TTATACAAAG TACAAAGTGG TGGTTTATTC AGCGGTAGTT 
AAGGCAGATG CAAAAACATA CAGGTTTAGG GATAAAGTGT TGAAGAAAGG TGGAGTTTGG 
AAGGATATAT ACGGGATAGA TGAGAAAAAG ATAGCAAGTA TGGTCAGAGA GGACAAAATC 
GACATTTTGG TAGAACTCAC TGGTCATACG GCAAACAACA AGTTGGGAAC AATGGCCTGC 
AGACCAGCAC CGGTTCAGGT TACTTGGATC GGCTATCCAA ATACTACGGG TTTGCCCACT 
GTTGATTACA GAATTACAGA TTCGTTGGCT GATCCACCAG ATACCAAACA AAAGCAGGTC 
GAGGAGCTGG TTAGGCTTCC GGACTGTTTT CTTTGTTATA CACCTTCCCC AGAGGCTGGT 
CCTGTTTGTC CAACACCTGC GCTTTCTAAT GGCTTTGTCA CATTTGGTAG TTTCAACAAC 
CTCGCAAAGA TAACTCCTAA GGTGCTGCAA GTGTGGGCTA GGATACTGTG TGCAGTTCCC 
AATTCTCGTC TAGTGGTAAA ATGCAAACCT TTCTGCTGCG ATAGCATTAG GCAGAGGTTT 
CTCACCACGC TGGAGCAGCT TGGGTTAGAA TCAAAGCGTG TTGATCTCTT GCCTTTGATT 
CTTTTCAATC ACGACCATAT GCAAGCCTAT TCCTTGATGG ATATTAGTTT GGACACATTC 
CCTTATGCTG GAACTACCAC TACCTGTGAG TCTCTCTACA TGGGAGTTCC ATGTGTTACA 
ATGGCTGGCT CAGTACATGC TCATAATGTT GGTGTCAGTC TTCTCACTAA AGTTGGATTA 
GGACACCTGG TTGCCAAAAA TGAGGATGAG TATGTTCAGC TATCTGTTGA TCTAGCCTCT 
GATGTCACAG CTCTTTCTAA ATTGAGAATG AGTCTCCGGG ATCTAATGGC TGGATCTCCT 
GTTTGTAATG GTCCTTCCTT TGCTGTTGGT CTTGAATCCG CATATCGGAA TATGTGGAAA 
AAGTACTGCA AAGGTGAAGT GCCGTCCTTA AGGCGAATGG AAATGCTGCA AAAAGAGGTC 
CATGATGATC CCTTAATCTC AAAAGACTTG GGACCATCAA GAGTCAGCGT TACTGGAGAA 
GCCACTCCGT CTCTCAAGGC CAATGGTTCT GCTCCTGTAC CTTCCTCTTT ACCAACCCAA 
TCCCCGCAGC TCTCAAAGAG AATGGACTCC ACTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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SPY cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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