LON4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	LON4
Entrez Gene ID	819748
Full Name	lon protease 4
Synonyms	F10A16.8,F10A16_8,lon protease 4
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001337610.1	NP_001325548.1	lon protease 4
NM_111453.1	NP_566259.1	lon protease 4

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	LON3	NP_566258.1
Arabidopsis thaliana (thale cress)	LON4	NP_566259.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lon protease-like protein 4 (Lon4) targeted to both mitochondria, chloroplasts, and is also located in thylakoids.[Lon4]
Title: Multiple intracellular locations of Lon protease in Arabidopsis: evidence for the localization of AtLon4 to chloroplasts.

The following LON4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LON4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb10321	NM_111453.1 Latest version!	Arabidopsis thaliana lon protease 4 (LON4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb37139	NM_001337610.1 Latest version!	Arabidopsis thaliana lon protease 4 (LON4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LON4 ( NM_111453.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_566259.1 Arabidopsis thaliana lon protease 4 (LON4), partial mRNA.

Price & Availability↑

CloneID	OAb10321
Clone ID Related Accession (Same CDS sequence)	NM_111453.1
Accession Version	NM_111453.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	lon protease 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). COMPLETENESS: incomplete on both ends.

ATGCTGAAGT TTCTCACTCC GACTGCTTAC GCTTCCCATC ACGTGACTCC GGCTACTCGA 
TTCCGGTCTA CCCCGGTTAA GAATCTATTG TTCAAGCAAC TGACTCTTCT AACCGGATGG 
AACCGGAGTT CCTACGAATT GGGTCGTCGT TCTTTTTCCT CCGACTTGGA TTCCGACACA 
AAGTCTTCAA CTACTACTGT CTCAGCTAAA CCTCACCTGG ACGATTGTCT AACGGTTATA 
GCATTGCCAT TGCCCCACAA GCCTCTTATT CCTGGCTTTT ACATGCCAAT ATATGTCAAG 
GATCCTAAAG TTCTTGCAGC TTTACAAGAG AGTAGAAGAC AACAAGCTCC ATATGCAGGG 
GCTTTCCTTT TGAAGGATGA CGCGTCATCT GACTCATCTT CGAGCTCTGA AACTGAGAAT 
ATTCTAGAGA AGTTGAAAGG AAAAGAGTTG ATTAACCGGA TTCATGAAGT TGGCACACTT 
GCCCAGATTT CAAGTATCCA AGGTGAGCAA GTCATCCTTA TTGGTCACAG ACAACTTCGA 
ATAACAGAGA TGGTGAGTGA GAGTGAAGAT CCCCTAACCG TCAAAGTTGA CCATCTAAAG 
GATAAGCCAT ATGACAAGGA CGATGATGTC ATCAAGGCAA CATACTTTCA AGTGATGTCG 
ACACTTAGGG ATGTCTTAAA GACAACTTCA CTCTGGAGAG ATCATGTTCG GACATATACC 
CAGGCATGTT CTCTTCATAT TTGGCACTGC TTGAGACATA TAGGTGAATT CAATTATCCA 
AAGTTAGCCG ATTTTGGAGC TGGGATCTCT GGCGCTAACA AACATCAAAA TCAGGGGGTT 
CTTGAAGAAC TGGATGTTCA CAAACGTCTA GAGTTGACGC TGGAATTGGT GAAAAAAGAA 
GTTGAAATTA ACAAGATTCA AGAATCCATA GCAAAAGCTG TTGAAGAAAA ATTTAGTGGC 
GATAGACGTC GTATCATACT AAAGGAGCAG ATCAATGCTA TAAAAAAGGA GCTTGGCGGG 
GAGACAGACA GCAAATCTGC ACTTTCTGAA AAGTTTAGGG GGAGGATTGA CCCTATTAAA 
GACAAGATTC CAGGACATGT ACTAAAAGTC ATAGAAGAAG AGCTTAAAAA ACTGCAGCTG 
TTAGAAACCA GTTCTAGCGA ATTCGATGTG ACTTGTAACT ACCTTGATTG GTTGACCGTG 
TTGCCTTGGG GAAATTTCAG TGATGAGAAT TTTAATGTCC TACGGGCAGA AAAGATTCTT 
GACGAGGATC ATTACGGATT ATCTGATGTA AAAGAAAGAA TACTAGAGTT TATTGCTGTG 
GGAGGACTTA GAGGCACTTC ACAAGGGAAG ATCATTTGTC TCTCTGGCCC AACTGGAGTA 
GGTAAAACTA GCATTGGGCG TTCAATTGCA CGTGCTCTTG ACCGCAAGTT CTTCCGGTTC 
TCTGTTGGAG GACTATCCGA TGTTGCTGAG ATTAAGGGGC ATCGTCGAAC GTATATTGGT 
GCCATGCCTG GAAAGATGGT GCAGTGTCTA AAGAATGTGG GAACAGAAAA TCCTCTTGTT 
CTGATCGATG AGATTGATAA GCTTGGAGTT AGGGGCCACC ATGGCGACCC AGCCAGTGCA 
ATGTTGGAGC TTTTGGATCC AGAGCAGAAT GCAAATTTTC TAGACCACTA TCTCGATGTT 
CCTATTGACT TATCCAAGGT TTTATTTGTA TGCACAGCAA ACGTGACAGA TACCATTCCG 
GGTCCTCTGC TAGACAGAAT GGAGGTGATC ACTCTCTCAG GGTACATTAC TGATGAGAAA 
ATGCATATCG CTAGAGACTA TTTGGAGAAA ACCGCACGCA GAGATTGTGG CATTAAGCCT 
GAACAGGTTG ATGTGAGCGA TGCAGCTTTT CTTTCCTTGA TAGAACATTA CTGCAGAGAA 
GCAGGAGTTA GAAATCTCCA GAAGCAGATT GAGAAGATTT TTCGTAAGAT TGCTCTTAAA 
CTTGTTCGCA AAGCGGCATC CACCGAAGTG CCTAGAATTT CTGATGATGT TACTACTGAC 
ACTGAAGAAA CTAAATCTTT GGCCAAGACT GATTTGGAAT CTCCAGAGAC CTCTGCAGAA 
GGATCCACTG TGTTGACAGA TGAGTTGGCA ACTGGGGATC CAACAGAATC AACGACTGAG 
CAGAGCGGAG AGGTAGCTGA AACGGTTGAA AAGTATATGA TTGATGAATC AAACCTTTCA 
GATTATGTAG GCAAGCCAGT ATTCCAGGAA GAGAAGATAT ATGAGCAGAC ACCAGTCGGG 
GTTGTAATGG GTCTAGCTTG GACATCCATG GGGGGCTCAA CATTGTACAT AGAGACAACC 
TTTGTAGAAG AAGGAGAAGG CAAAGGTGGT CTTCATATAA CGGGTCGGCT TGGGGATGTG 
ATGAAAGAAA GCGCAGAGAT AGCTCACACA GTCGCGAGAA GAATAATGCT GGAGAAAGAA 
CCCGAGAACA AGTTGTTTGC GAATTCCAAG CTTCATCTAC ATGTACCTGC AGGAGCCACA 
CCAAAAGACG GCCCAAGCGC AGGCTGCACC ATGATAACTT CATTGCTATC ACTTGCCTTG 
AAGAAGCCTG TAAGGAAGGA TCTTGCAATG ACTGGAGAAG TCACTTTGAC CGGTAGAATC 
CTTGCGATTG GCGGCGTGAA AGAGAAGACT ATAGCTGCAA GACGGAGTCA AGTGAAGGTG 
ATAATATTCC CTGAGGCTAA CCGGAGAGAT TTTGATGAGC TGGCGAGAAA CGTGAAAGAA 
GGGCTCGAAG TTCATTTCGT GGATGAATAT GAGCAGATTT TCGAGCTAGC CTTTGGCTAT 
GACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_566259.1
CDS	1..2829
Translation	MLKFLTPTAYASHHVTPATRFRSTPVKNLLFKQLTLLTGWNRSSYELGRRSFSSDLDSDTKSSTTTVSAKPHLDDCLTVIALPLPHKPLIPGFYMPIYVKDPKVLAALQESRRQQAPYAGAFLLKDDASSDSSSSSETENILEKLKGKELINRIHEVGTLAQISSIQGEQVILIGHRQLRITEMVSESEDPLTVKVDHLKDKPYDKDDDVIKATYFQVMSTLRDVLKTTSLWRDHVRTYTQACSLHIWHCLRHIGEFNYPKLADFGAGISGANKHQNQGVLEELDVHKRLELTLELVKKEVEINKIQESIAKAVEEKFSGDRRRIILKEQINAIKKELGGETDSKSALSEKFRGRIDPIKDKIPGHVLKVIEEELKKLQLLETSSSEFDVTCNYLDWLTVLPWGNFSDENFNVLRAEKILDEDHYGLSDVKERILEFIAVGGLRGTSQGKIICLSGPTGVGKTSIGRSIARALDRKFFRFSVGGLSDVAEIKGHRRTYIGAMPGKMVQCLKNVGTENPLVLIDEIDKLGVRGHHGDPASAMLELLDPEQNANFLDHYLDVPIDLSKVLFVCTANVTDTIPGPLLDRMEVITLSGYITDEKMHIARDYLEKTARRDCGIKPEQVDVSDAAFLSLIEHYCREAGVRNLQKQIEKIFRKIALKLVRKAASTEVPRISDDVTTDTEETKSLAKTDLESPETSAEGSTVLTDELATGDPTESTTEQSGEVAETVEKYMIDESNLSDYVGKPVFQEEKIYEQTPVGVVMGLAWTSMGGSTLYIETTFVEEGEGKGGLHITGRLGDVMKESAEIAHTVARRIMLEKEPENKLFANSKLHLHVPAGATPKDGPSAGCTMITSLLSLALKKPVRKDLAMTGEVTLTGRILAIGGVKEKTIAARRSQVKVIIFPEANRRDFDELARNVKEGLEVHFVDEYEQIFELAFGYDH

Target ORF information:

RefSeq Version	NM_111453.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana lon protease 4 (LON4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_111453.1

ORF Insert Sequence:

ATGCTGAAGT TTCTCACTCC GACTGCTTAC GCTTCCCATC ACGTGACTCC GGCTACTCGA 
TTCCGGTCTA CCCCGGTTAA GAATCTATTG TTCAAGCAAC TGACTCTTCT AACCGGATGG 
AACCGGAGTT CCTACGAATT GGGTCGTCGT TCTTTTTCCT CCGACTTGGA TTCCGACACA 
AAGTCTTCAA CTACTACTGT CTCAGCTAAA CCTCACCTGG ACGATTGTCT AACGGTTATA 
GCATTGCCAT TGCCCCACAA GCCTCTTATT CCTGGCTTTT ACATGCCAAT ATATGTCAAG 
GATCCTAAAG TTCTTGCAGC TTTACAAGAG AGTAGAAGAC AACAAGCTCC ATATGCAGGG 
GCTTTCCTTT TGAAGGATGA CGCGTCATCT GACTCATCTT CGAGCTCTGA AACTGAGAAT 
ATTCTAGAGA AGTTGAAAGG AAAAGAGTTG ATTAACCGGA TTCATGAAGT TGGCACACTT 
GCCCAGATTT CAAGTATCCA AGGTGAGCAA GTCATCCTTA TTGGTCACAG ACAACTTCGA 
ATAACAGAGA TGGTGAGTGA GAGTGAAGAT CCCCTAACCG TCAAAGTTGA CCATCTAAAG 
GATAAGCCAT ATGACAAGGA CGATGATGTC ATCAAGGCAA CATACTTTCA AGTGATGTCG 
ACACTTAGGG ATGTCTTAAA GACAACTTCA CTCTGGAGAG ATCATGTTCG GACATATACC 
CAGGCATGTT CTCTTCATAT TTGGCACTGC TTGAGACATA TAGGTGAATT CAATTATCCA 
AAGTTAGCCG ATTTTGGAGC TGGGATCTCT GGCGCTAACA AACATCAAAA TCAGGGGGTT 
CTTGAAGAAC TGGATGTTCA CAAACGTCTA GAGTTGACGC TGGAATTGGT GAAAAAAGAA 
GTTGAAATTA ACAAGATTCA AGAATCCATA GCAAAAGCTG TTGAAGAAAA ATTTAGTGGC 
GATAGACGTC GTATCATACT AAAGGAGCAG ATCAATGCTA TAAAAAAGGA GCTTGGCGGG 
GAGACAGACA GCAAATCTGC ACTTTCTGAA AAGTTTAGGG GGAGGATTGA CCCTATTAAA 
GACAAGATTC CAGGACATGT ACTAAAAGTC ATAGAAGAAG AGCTTAAAAA ACTGCAGCTG 
TTAGAAACCA GTTCTAGCGA ATTCGATGTG ACTTGTAACT ACCTTGATTG GTTGACCGTG 
TTGCCTTGGG GAAATTTCAG TGATGAGAAT TTTAATGTCC TACGGGCAGA AAAGATTCTT 
GACGAGGATC ATTACGGATT ATCTGATGTA AAAGAAAGAA TACTAGAGTT TATTGCTGTG 
GGAGGACTTA GAGGCACTTC ACAAGGGAAG ATCATTTGTC TCTCTGGCCC AACTGGAGTA 
GGTAAAACTA GCATTGGGCG TTCAATTGCA CGTGCTCTTG ACCGCAAGTT CTTCCGGTTC 
TCTGTTGGAG GACTATCCGA TGTTGCTGAG ATTAAGGGGC ATCGTCGAAC GTATATTGGT 
GCCATGCCTG GAAAGATGGT GCAGTGTCTA AAGAATGTGG GAACAGAAAA TCCTCTTGTT 
CTGATCGATG AGATTGATAA GCTTGGAGTT AGGGGCCACC ATGGCGACCC AGCCAGTGCA 
ATGTTGGAGC TTTTGGATCC AGAGCAGAAT GCAAATTTTC TAGACCACTA TCTCGATGTT 
CCTATTGACT TATCCAAGGT TTTATTTGTA TGCACAGCAA ACGTGACAGA TACCATTCCG 
GGTCCTCTGC TAGACAGAAT GGAGGTGATC ACTCTCTCAG GGTACATTAC TGATGAGAAA 
ATGCATATCG CTAGAGACTA TTTGGAGAAA ACCGCACGCA GAGATTGTGG CATTAAGCCT 
GAACAGGTTG ATGTGAGCGA TGCAGCTTTT CTTTCCTTGA TAGAACATTA CTGCAGAGAA 
GCAGGAGTTA GAAATCTCCA GAAGCAGATT GAGAAGATTT TTCGTAAGAT TGCTCTTAAA 
CTTGTTCGCA AAGCGGCATC CACCGAAGTG CCTAGAATTT CTGATGATGT TACTACTGAC 
ACTGAAGAAA CTAAATCTTT GGCCAAGACT GATTTGGAAT CTCCAGAGAC CTCTGCAGAA 
GGATCCACTG TGTTGACAGA TGAGTTGGCA ACTGGGGATC CAACAGAATC AACGACTGAG 
CAGAGCGGAG AGGTAGCTGA AACGGTTGAA AAGTATATGA TTGATGAATC AAACCTTTCA 
GATTATGTAG GCAAGCCAGT ATTCCAGGAA GAGAAGATAT ATGAGCAGAC ACCAGTCGGG 
GTTGTAATGG GTCTAGCTTG GACATCCATG GGGGGCTCAA CATTGTACAT AGAGACAACC 
TTTGTAGAAG AAGGAGAAGG CAAAGGTGGT CTTCATATAA CGGGTCGGCT TGGGGATGTG 
ATGAAAGAAA GCGCAGAGAT AGCTCACACA GTCGCGAGAA GAATAATGCT GGAGAAAGAA 
CCCGAGAACA AGTTGTTTGC GAATTCCAAG CTTCATCTAC ATGTACCTGC AGGAGCCACA 
CCAAAAGACG GCCCAAGCGC AGGCTGCACC ATGATAACTT CATTGCTATC ACTTGCCTTG 
AAGAAGCCTG TAAGGAAGGA TCTTGCAATG ACTGGAGAAG TCACTTTGAC CGGTAGAATC 
CTTGCGATTG GCGGCGTGAA AGAGAAGACT ATAGCTGCAA GACGGAGTCA AGTGAAGGTG 
ATAATATTCC CTGAGGCTAA CCGGAGAGAT TTTGATGAGC TGGCGAGAAA CGTGAAAGAA 
GGGCTCGAAG TTCATTTCGT GGATGAATAT GAGCAGATTT TCGAGCTAGC CTTTGGCTAT 
GACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LON4 ( NM_001337610.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001325548.1 Arabidopsis thaliana lon protease 4 (LON4), mRNA.

Price & Availability↑

CloneID	OAb37139
Clone ID Related Accession (Same CDS sequence)	NM_001337610.1
Accession Version	NM_001337610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	lon protease 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGCTGAAGT TTCTCACTCC GACTGCTTAC GCTTCCCATC ACGTGACTCC GGCTACTCGA 
TTCCGGTCTA CCCCGGTTAA GAATCTATTG TTCAAGCAAC TGACTCTTCT AACCGGATGG 
AACCGGAGTT CCTACGAATT GGGTCGTCGT TCTTTTTCCT CCGACTTGGA TTCCGACACA 
AAGTCTTCAA CTACTACTGT CTCAGCTAAA CCTCACCTGG ACGATTGTCT AACGGTTATA 
GCATTGCCAT TGCCCCACAA GCCTCTTATT CCTGGCTTTT ACATGCCAAT ATATGTCAAG 
GATCCTAAAG TTCTTGCAGC TTTACAAGAG AGTAGAAGAC AACAAGCTCC ATATGCAGGG 
GCTTTCCTTT TGAAGGATGA CGCGTCATCT GACTCATCTT CGAGCTCTGA AACTGAGAAT 
ATTCTAGAGA AGTTGAAAGG AAAAGAGTTG ATTAACCGGA TTCATGAAGT TGGCACACTT 
GCCCAGATTT CAAGTATCCA AGGTGAGCAA GTCATCCTTA TTGGTCACAG ACAACTTCGA 
ATAACAGAGA TGGTGAGTGA GAGTGAAGAT CCCCTAACCG TCAAAGTTGA CCATCTAAAG 
GATAAGCCAT ATGACAAGGA CGATGATGTC ATCAAGGCAA CATACTTTCA AGTGATGTCG 
ACACTTAGGG ATGTCTTAAA GACAACTTCA CTCTGGAGAG ATCATGTTCG GACATATACC 
CAGCATATAG GTGAATTCAA TTATCCAAAG TTAGCCGATT TTGGAGCTGG GATCTCTGGC 
GCTAACAAAC ATCAAAATCA GGGGGTTCTT GAAGAACTGG ATGTTCACAA ACGTCTAGAG 
TTGACGCTGG AATTGGTGAA AAAAGAAGTT GAAATTAACA AGATTCAAGA ATCCATAGCA 
AAAGCTGTTG AAGAAAAATT TAGTGGCGAT AGACGTCGTA TCATACTAAA GGAGCAGATC 
AATGCTATAA AAAAGGAGCT TGGCGGGGAG ACAGACAGCA AATCTGCACT TTCTGAAAAG 
TTTAGGGGGA GGATTGACCC TATTAAAGAC AAGATTCCAG GACATGTACT AAAAGTCATA 
GAAGAAGAGC TTAAAAAACT GCAGCTGTTA GAAACCAGTT CTAGCGAATT CGATGTGACT 
TGTAACTACC TTGATTGGTT GACCGTGTTG CCTTGGGGAA ATTTCAGTGA TGAGAATTTT 
AATGTCCTAC GGGCAGAAAA GATTCTTGAC GAGGATCATT ACGGATTATC TGATGTAAAA 
GAAAGAATAC TAGAGTTTAT TGCTGTGGGA GGACTTAGAG GCACTTCACA AGGGAAGATC 
ATTTGTCTCT CTGGCCCAAC TGGAGTAGGT AAAACTAGCA TTGGGCGTTC AATTGCACGT 
GCTCTTGACC GCAAGTTCTT CCGGTTCTCT GTTGGAGGAC TATCCGATGT TGCTGAGATT 
AAGGGGCATC GTCGAACGTA TATTGGTGCC ATGCCTGGAA AGATGGTGCA GTGTCTAAAG 
AATGTGGGAA CAGAAAATCC TCTTGTTCTG ATCGATGAGA TTGATAAGCT TGGAGTTAGG 
GGCCACCATG GCGACCCAGC CAGTGCAATG TTGGAGCTTT TGGATCCAGA GCAGAATGCA 
AATTTTCTAG ACCACTATCT CGATGTTCCT ATTGACTTAT CCAAGGTTTT ATTTGTATGC 
ACAGCAAACG TGACAGATAC CATTCCGGGT CCTCTGCTAG ACAGAATGGA GGTGATCACT 
CTCTCAGGGT ACATTACTGA TGAGAAAATG CATATCGCTA GAGACTATTT GGAGAAAACC 
GCACGCAGAG ATTGTGGCAT TAAGCCTGAA CAGGTTGATG TGAGCGATGC AGCTTTTCTT 
TCCTTGATAG AACATTACTG CAGAGAAGCA GGAGTTAGAA ATCTCCAGAA GCAGATTGAG 
AAGATTTTTC GTAAGATTGC TCTTAAACTT GTTCGCAAAG CGGCATCCAC CGAAGTGCCT 
AGAATTTCTG ATGATGTTAC TACTGACACT GAAGAAACTA AATCTTTGGC CAAGACTGAT 
TTGGAATCTC CAGAGACCTC TGCAGAAGGA TCCACTGTGT TGACAGATGA GTTGGCAACT 
GGGGATCCAA CAGAATCAAC GACTGAGCAG AGCGGAGAGG TAGCTGAAAC GGTTGAAAAG 
TATATGATTG ATGAATCAAA CCTTTCAGAT TATGTAGGCA AGCCAGTATT CCAGGAAGAG 
AAGATATATG AGCAGACACC AGTCGGGGTT GTAATGGGTC TAGCTTGGAC ATCCATGGGG 
GGCTCAACAT TGTACATAGA GACAACCTTT GTAGAAGAAG GAGAAGGCAA AGGTGGTCTT 
CATATAACGG GTCGGCTTGG GGATGTGATG AAAGAAAGCG CAGAGATAGC TCACACAGTC 
GCGAGAAGAA TAATGCTGGA GAAAGAACCC GAGAACAAGT TGTTTGCGAA TTCCAAGCTT 
CATCTACATG TACCTGCAGG AGCCACACCA AAAGACGGCC CAAGCGCAGG CTGCACCATG 
ATAACTTCAT TGCTATCACT TGCCTTGAAG AAGCCTGTAA GGAAGGATCT TGCAATGACT 
GGAGAAGTCA CTTTGACCGG TAGAATCCTT GCGATTGGCG GCGTGAAAGA GAAGACTATA 
GCTGCAAGAC GGAGTCAAGT GAAGGTGATA ATATTCCCTG AGGCTAACCG GAGAGATTTT 
GATGAGCTGG CGAGAAACGT GAAAGAAGGG CTCGAAGTTC ATTTCGTGGA TGAATATGAG 
CAGATTTTCG AGCTAGCCTT TGGCTATGAC CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001325548.1
CDS	87..2882
Translation	MLKFLTPTAYASHHVTPATRFRSTPVKNLLFKQLTLLTGWNRSSYELGRRSFSSDLDSDTKSSTTTVSAKPHLDDCLTVIALPLPHKPLIPGFYMPIYVKDPKVLAALQESRRQQAPYAGAFLLKDDASSDSSSSSETENILEKLKGKELINRIHEVGTLAQISSIQGEQVILIGHRQLRITEMVSESEDPLTVKVDHLKDKPYDKDDDVIKATYFQVMSTLRDVLKTTSLWRDHVRTYTQHIGEFNYPKLADFGAGISGANKHQNQGVLEELDVHKRLELTLELVKKEVEINKIQESIAKAVEEKFSGDRRRIILKEQINAIKKELGGETDSKSALSEKFRGRIDPIKDKIPGHVLKVIEEELKKLQLLETSSSEFDVTCNYLDWLTVLPWGNFSDENFNVLRAEKILDEDHYGLSDVKERILEFIAVGGLRGTSQGKIICLSGPTGVGKTSIGRSIARALDRKFFRFSVGGLSDVAEIKGHRRTYIGAMPGKMVQCLKNVGTENPLVLIDEIDKLGVRGHHGDPASAMLELLDPEQNANFLDHYLDVPIDLSKVLFVCTANVTDTIPGPLLDRMEVITLSGYITDEKMHIARDYLEKTARRDCGIKPEQVDVSDAAFLSLIEHYCREAGVRNLQKQIEKIFRKIALKLVRKAASTEVPRISDDVTTDTEETKSLAKTDLESPETSAEGSTVLTDELATGDPTESTTEQSGEVAETVEKYMIDESNLSDYVGKPVFQEEKIYEQTPVGVVMGLAWTSMGGSTLYIETTFVEEGEGKGGLHITGRLGDVMKESAEIAHTVARRIMLEKEPENKLFANSKLHLHVPAGATPKDGPSAGCTMITSLLSLALKKPVRKDLAMTGEVTLTGRILAIGGVKEKTIAARRSQVKVIIFPEANRRDFDELARNVKEGLEVHFVDEYEQIFELAFGYDH

Target ORF information:

RefSeq Version	NM_001337610.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana lon protease 4 (LON4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001337610.1

ORF Insert Sequence:

ATGCTGAAGT TTCTCACTCC GACTGCTTAC GCTTCCCATC ACGTGACTCC GGCTACTCGA 
TTCCGGTCTA CCCCGGTTAA GAATCTATTG TTCAAGCAAC TGACTCTTCT AACCGGATGG 
AACCGGAGTT CCTACGAATT GGGTCGTCGT TCTTTTTCCT CCGACTTGGA TTCCGACACA 
AAGTCTTCAA CTACTACTGT CTCAGCTAAA CCTCACCTGG ACGATTGTCT AACGGTTATA 
GCATTGCCAT TGCCCCACAA GCCTCTTATT CCTGGCTTTT ACATGCCAAT ATATGTCAAG 
GATCCTAAAG TTCTTGCAGC TTTACAAGAG AGTAGAAGAC AACAAGCTCC ATATGCAGGG 
GCTTTCCTTT TGAAGGATGA CGCGTCATCT GACTCATCTT CGAGCTCTGA AACTGAGAAT 
ATTCTAGAGA AGTTGAAAGG AAAAGAGTTG ATTAACCGGA TTCATGAAGT TGGCACACTT 
GCCCAGATTT CAAGTATCCA AGGTGAGCAA GTCATCCTTA TTGGTCACAG ACAACTTCGA 
ATAACAGAGA TGGTGAGTGA GAGTGAAGAT CCCCTAACCG TCAAAGTTGA CCATCTAAAG 
GATAAGCCAT ATGACAAGGA CGATGATGTC ATCAAGGCAA CATACTTTCA AGTGATGTCG 
ACACTTAGGG ATGTCTTAAA GACAACTTCA CTCTGGAGAG ATCATGTTCG GACATATACC 
CAGCATATAG GTGAATTCAA TTATCCAAAG TTAGCCGATT TTGGAGCTGG GATCTCTGGC 
GCTAACAAAC ATCAAAATCA GGGGGTTCTT GAAGAACTGG ATGTTCACAA ACGTCTAGAG 
TTGACGCTGG AATTGGTGAA AAAAGAAGTT GAAATTAACA AGATTCAAGA ATCCATAGCA 
AAAGCTGTTG AAGAAAAATT TAGTGGCGAT AGACGTCGTA TCATACTAAA GGAGCAGATC 
AATGCTATAA AAAAGGAGCT TGGCGGGGAG ACAGACAGCA AATCTGCACT TTCTGAAAAG 
TTTAGGGGGA GGATTGACCC TATTAAAGAC AAGATTCCAG GACATGTACT AAAAGTCATA 
GAAGAAGAGC TTAAAAAACT GCAGCTGTTA GAAACCAGTT CTAGCGAATT CGATGTGACT 
TGTAACTACC TTGATTGGTT GACCGTGTTG CCTTGGGGAA ATTTCAGTGA TGAGAATTTT 
AATGTCCTAC GGGCAGAAAA GATTCTTGAC GAGGATCATT ACGGATTATC TGATGTAAAA 
GAAAGAATAC TAGAGTTTAT TGCTGTGGGA GGACTTAGAG GCACTTCACA AGGGAAGATC 
ATTTGTCTCT CTGGCCCAAC TGGAGTAGGT AAAACTAGCA TTGGGCGTTC AATTGCACGT 
GCTCTTGACC GCAAGTTCTT CCGGTTCTCT GTTGGAGGAC TATCCGATGT TGCTGAGATT 
AAGGGGCATC GTCGAACGTA TATTGGTGCC ATGCCTGGAA AGATGGTGCA GTGTCTAAAG 
AATGTGGGAA CAGAAAATCC TCTTGTTCTG ATCGATGAGA TTGATAAGCT TGGAGTTAGG 
GGCCACCATG GCGACCCAGC CAGTGCAATG TTGGAGCTTT TGGATCCAGA GCAGAATGCA 
AATTTTCTAG ACCACTATCT CGATGTTCCT ATTGACTTAT CCAAGGTTTT ATTTGTATGC 
ACAGCAAACG TGACAGATAC CATTCCGGGT CCTCTGCTAG ACAGAATGGA GGTGATCACT 
CTCTCAGGGT ACATTACTGA TGAGAAAATG CATATCGCTA GAGACTATTT GGAGAAAACC 
GCACGCAGAG ATTGTGGCAT TAAGCCTGAA CAGGTTGATG TGAGCGATGC AGCTTTTCTT 
TCCTTGATAG AACATTACTG CAGAGAAGCA GGAGTTAGAA ATCTCCAGAA GCAGATTGAG 
AAGATTTTTC GTAAGATTGC TCTTAAACTT GTTCGCAAAG CGGCATCCAC CGAAGTGCCT 
AGAATTTCTG ATGATGTTAC TACTGACACT GAAGAAACTA AATCTTTGGC CAAGACTGAT 
TTGGAATCTC CAGAGACCTC TGCAGAAGGA TCCACTGTGT TGACAGATGA GTTGGCAACT 
GGGGATCCAA CAGAATCAAC GACTGAGCAG AGCGGAGAGG TAGCTGAAAC GGTTGAAAAG 
TATATGATTG ATGAATCAAA CCTTTCAGAT TATGTAGGCA AGCCAGTATT CCAGGAAGAG 
AAGATATATG AGCAGACACC AGTCGGGGTT GTAATGGGTC TAGCTTGGAC ATCCATGGGG 
GGCTCAACAT TGTACATAGA GACAACCTTT GTAGAAGAAG GAGAAGGCAA AGGTGGTCTT 
CATATAACGG GTCGGCTTGG GGATGTGATG AAAGAAAGCG CAGAGATAGC TCACACAGTC 
GCGAGAAGAA TAATGCTGGA GAAAGAACCC GAGAACAAGT TGTTTGCGAA TTCCAAGCTT 
CATCTACATG TACCTGCAGG AGCCACACCA AAAGACGGCC CAAGCGCAGG CTGCACCATG 
ATAACTTCAT TGCTATCACT TGCCTTGAAG AAGCCTGTAA GGAAGGATCT TGCAATGACT 
GGAGAAGTCA CTTTGACCGG TAGAATCCTT GCGATTGGCG GCGTGAAAGA GAAGACTATA 
GCTGCAAGAC GGAGTCAAGT GAAGGTGATA ATATTCCCTG AGGCTAACCG GAGAGATTTT 
GATGAGCTGG CGAGAAACGT GAAAGAAGGG CTCGAAGTTC ATTTCGTGGA TGAATATGAG 
CAGATTTTCG AGCTAGCCTT TGGCTATGAC CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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LON4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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