HMBS cDNA ORF clone, Apteryx australis mantelli

Gene
Clones
gRNAs

Summary

Gene Symbol	HMBS
Entrez Gene ID	106485201
Full Name	hydroxymethylbilane synthase
Gene Type	protein-coding
Organism	Apteryx australis mantelli

mRNA and Protein(s)

mRNA	Protein	Name
XM_013943514.1	XP_013798968.1	LOW QUALITY PROTEIN: porphobilinogen deaminase

Pathways from BioSystems
Gene Ontology

The following HMBS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HMBS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAp14775	XM_013943514.1 Latest version!	Apteryx australis mantelli hydroxymethylbilane synthase (HMBS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HMBS ( XM_013943514.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013798968.1 Apteryx australis mantelli hydroxymethylbilane synthase (HMBS), mRNA.

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CloneID	OAp14775
Clone ID Related Accession (Same CDS sequence)	XM_013943514.1
Accession Version	XM_013943514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	LOW QUALITY PROTEIN: porphobilinogen deaminase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013991808.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCTACTTG TTGTTAATGC TTATGTTTTA GAGGATGTCA TGGTTATGAG TGACTATCAA 
AGAAGGCTAA AGGCCTCTCT AGCTACTGTA TCAATGATGC ACTTTTTGCT TGGAACTTTG 
CTTTCTGCTT TATTGCAGCT GGCCCGGATT CAGACTGACA GTGTAGTTGA GATGCTCCGT 
GAGCTATATC CTGACCTCCA CTATGAGATT GTTGCCATGT CAACAACGGG AGACAAGATC 
TTGGATACAG CACTTTCCAA GATCGGGGAG AAGAGCCTCT TCACCAAAGA GCTGGAAAAT 
GCCCTTGAAA GAAATGAAGT TGACCTGGTG GTTCACTCCT TGAAGGACCT GCCAACTTCT 
CTCCCTCCTG GGTTTACCAT TGGTGCTATC TGCAAAAGGG AAAACCCACA TGATGCTGTT 
GTCTTTCATC CCAAAAATTC TGGGAAAATG CTTAGCCTTC TTCCTGAAAA GAGTGTAATT 
GGAACCAGTT CACTTCGGCG AGCAGCCCAA CTCAAAAAGA AGTTCCCTCA GTTAGAATTC 
AGGGATATCA GAGGGAATTT AAACACCCGC TTGAAGAAAC TTGATGAGAA GGAAGACTTC 
AGTGCCATAA TCCTGGCTGC TGCCGGGCTG AAGAGAATGG GCTGGGAGAA TCGCATTGGC 
CAGATCCTAA GCCCGGAAGA TTGTCTCTAT GCTGTTGGAC AGGGTGCCTT AGCAGTGGAA 
GTTCGTGCCA AAGACCAAGA GATACTGAAT ATGGTATCTG CCCTGCATGA TGGAGAAACT 
GTGCTATGCT GCATTGCTGA GAGAGCCTTT ATGAAACATT TGGAGGGTGG ATGTAGTGTT 
CCTGTTGCAG TCAGCAGCAT GCTGAAAGAT GGCCAGTTGT ATTTGACTGG AGCAGTCTAC 
AGCTTGGATG GTTCTGATAG CCTGAAGGAG ACTATGCAGA CCAGTGTTAA TTATCCACAA 
CAGAATGAAG ATGGACCAAA TGACGATGTG CAGCATGTTG GCATCACAGC CAAGACTGTT 
CCTTGTCAGG CCCAGCAAGC TGCAGAGAAC CTTGGTGCTG AACTTGCTAG TTTACTTCTG 
AGCAAGGGAG CTAAACATAT CCTTAGTGTA GCAAGGCAGC TCAATGATGC TTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013798968.1
CDS	1..1137
Translation	MLLVVNAYVLEDVMVMSDYQRRLKASLATVSMMHFLLGTLLSALLQLARIQTDSVVEMLRELYPDLHYEIVAMSTTGDKILDTALSKIGEKSLFTKELENALERNEVDLVVHSLKDLPTSLPPGFTIGAICKRENPHDAVVFHPKNSGKMLSLLPEKSVIGTSSLRRAAQLKKKFPQLEFRDIRGNLNTRLKKLDEKEDFSAIILAAAGLKRMGWENRIGQILSPEDCLYAVGQGALAVEVRAKDQEILNMVSALHDGETVLCCIAERAFMKHLEGGCSVPVAVSSMLKDGQLYLTGAVYSLDGSDSLKETMQTSVNYPQQNEDGPNDDVQHVGITAKTVPCQAQQAAENLGAELASLLLSKGAKHILSVARQLNDAC

Target ORF information:

RefSeq Version	XM_013943514.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli hydroxymethylbilane synthase (HMBS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013943514.1

ORF Insert Sequence:

ATGCTACTTG TTGTTAATGC TTATGTTTTA GAGGATGTCA TGGTTATGAG TGACTATCAA 
AGAAGGCTAA AGGCCTCTCT AGCTACTGTA TCAATGATGC ACTTTTTGCT TGGAACTTTG 
CTTTCTGCTT TATTGCAGCT GGCCCGGATT CAGACTGACA GTGTAGTTGA GATGCTCCGT 
GAGCTATATC CTGACCTCCA CTATGAGATT GTTGCCATGT CAACAACGGG AGACAAGATC 
TTGGATACAG CACTTTCCAA GATCGGGGAG AAGAGCCTCT TCACCAAAGA GCTGGAAAAT 
GCCCTTGAAA GAAATGAAGT TGACCTGGTG GTTCACTCCT TGAAGGACCT GCCAACTTCT 
CTCCCTCCTG GGTTTACCAT TGGTGCTATC TGCAAAAGGG AAAACCCACA TGATGCTGTT 
GTCTTTCATC CCAAAAATTC TGGGAAAATG CTTAGCCTTC TTCCTGAAAA GAGTGTAATT 
GGAACCAGTT CACTTCGGCG AGCAGCCCAA CTCAAAAAGA AGTTCCCTCA GTTAGAATTC 
AGGGATATCA GAGGGAATTT AAACACCCGC TTGAAGAAAC TTGATGAGAA GGAAGACTTC 
AGTGCCATAA TCCTGGCTGC TGCCGGGCTG AAGAGAATGG GCTGGGAGAA TCGCATTGGC 
CAGATCCTAA GCCCGGAAGA TTGTCTCTAT GCTGTTGGAC AGGGTGCCTT AGCAGTGGAA 
GTTCGTGCCA AAGACCAAGA GATACTGAAT ATGGTATCTG CCCTGCATGA TGGAGAAACT 
GTGCTATGCT GCATTGCTGA GAGAGCCTTT ATGAAACATT TGGAGGGTGG ATGTAGTGTT 
CCTGTTGCAG TCAGCAGCAT GCTGAAAGAT GGCCAGTTGT ATTTGACTGG AGCAGTCTAC 
AGCTTGGATG GTTCTGATAG CCTGAAGGAG ACTATGCAGA CCAGTGTTAA TTATCCACAA 
CAGAATGAAG ATGGACCAAA TGACGATGTG CAGCATGTTG GCATCACAGC CAAGACTGTT 
CCTTGTCAGG CCCAGCAAGC TGCAGAGAAC CTTGGTGCTG AACTTGCTAG TTTACTTCTG 
AGCAAGGGAG CTAAACATAT CCTTAGTGTA GCAAGGCAGC TCAATGATGC TTGCTAA