LOC100640884 cDNA ORF clone, Amphimedon queenslandica

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100640884
Entrez Gene ID	100640884
Full Name	translation initiation factor eIF-2B subunit beta-like
Gene Type	protein-coding
Organism	Amphimedon queenslandica

mRNA and Protein(s)

mRNA	Protein	Name
XM_003388042.3	XP_003388090.1	translation initiation factor eIF-2B subunit beta-like isoform X2
XM_019998962.1	XP_019854521.1	translation initiation factor eIF-2B subunit beta-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100640884 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100640884 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm20870	XM_019998962.1 Latest version!	Amphimedon queenslandica translation initiation factor eIF-2B subunit beta-like (LOC100640884), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OAm20871	XM_003388042.3 Latest version!	Amphimedon queenslandica translation initiation factor eIF-2B subunit beta-like (LOC100640884), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100640884 ( XM_019998962.1 ) cDNA ORF clone, Amphimedon queenslandica -> XP_019854521.1 Amphimedon queenslandica translation initiation factor eIF-2B subunit beta-like (LOC100640884), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAm20870
Clone ID Related Accession (Same CDS sequence)	XM_019998962.1
Accession Version	XM_019998962.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-17
Organism	Amphimedon queenslandica
Product	translation initiation factor eIF-2B subunit beta-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003546466.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Amphimedon queenslandica Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAG AATCATTACA GCTCAAGCCG GGGAAAATGT TGTGTGTAAT ATGGTCAGAA 
GAGTTCTTAA AATTATTCGC GAAGAACTTG TGTCTGAGAG GATCTGATTC TGAGAGCATG 
GACCTCAAGG ATTCGTTACA TACTATGCTA AAAGGAGGTG AAGATTACGA TGATCTATCA 
AAGCCTGTTC CTAATTTAAA AGGTGCTGTC ATTGAAGCCA TACACGAATT ATTAGGAGAA 
ATGGAAGAAA GTGCATCCAA TATTGCAGCA CAAGCATTGG ATCACATTCA TTCCAATGAG 
GTCATTATGA CGTCAGGGCA TTCTAAAACA GTAGAAGCAT TTCTTAAAGG TGCTGCTAGA 
AAGAGAAAAT TTCAAGTGAT TGTAGCGGAA TCATCACCCT CTTATCAAGG TCAAAAACTA 
GCTCGTAATT TAGCTGAAGC AAAAATCGAA ACGACCGTAA TTACCGACTC GGCAGTTTTT 
GCTCTGATGT CAAGAGTTAA TAAGGTGATA ATCGGAACTC ATACAGTAAT GGCTGATGGA 
GGATTAAAAG CTTTAAATGG AGCACATGCT TTAACATTAG CAGCAAAGCA TCACTCAGTT 
CCAGTTATTG TTTGTGCTGC CATGTTCAAA CTCTCTCCAA AATTTCTCTG CTCATACGAC 
CAAGATACAT TTAACAAGAT AGTGTCACCT CATGATATAC TGGGATTTAC TGAGGCCGAC 
CTAGTATCCA AGGTTCATGT ACAGAATCCT GTATTCGACT ACGTCCCTCC CGATCTAATT 
AATTTATTTA TTTCTAATAT TGGTGGTAAT GCCCCCTCTT ATGTGTACAG ACTGATCAGT 
GAACTCTATG ATGATGACGA CTCAGATTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019854521.1
CDS	111..983
Translation	MGRESLQLKPGKMLCVIWSEEFLKLFAKNLCLRGSDSESMDLKDSLHTMLKGGEDYDDLSKPVPNLKGAVIEAIHELLGEMEESASNIAAQALDHIHSNEVIMTSGHSKTVEAFLKGAARKRKFQVIVAESSPSYQGQKLARNLAEAKIETTVITDSAVFALMSRVNKVIIGTHTVMADGGLKALNGAHALTLAAKHHSVPVIVCAAMFKLSPKFLCSYDQDTFNKIVSPHDILGFTEADLVSKVHVQNPVFDYVPPDLINLFISNIGGNAPSYVYRLISELYDDDDSDL

Target ORF information:

RefSeq Version	XM_019998962.1
Organism	Amphimedon queenslandica
Definition	Amphimedon queenslandica translation initiation factor eIF-2B subunit beta-like (LOC100640884), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019998962.1

ORF Insert Sequence:

ATGGGAAGAG AATCATTACA GCTCAAGCCG GGGAAAATGT TGTGTGTAAT ATGGTCAGAA 
GAGTTCTTAA AATTATTCGC GAAGAACTTG TGTCTGAGAG GATCTGATTC TGAGAGCATG 
GACCTCAAGG ATTCGTTACA TACTATGCTA AAAGGAGGTG AAGATTACGA TGATCTATCA 
AAGCCTGTTC CTAATTTAAA AGGTGCTGTC ATTGAAGCCA TACACGAATT ATTAGGAGAA 
ATGGAAGAAA GTGCATCCAA TATTGCAGCA CAAGCATTGG ATCACATTCA TTCCAATGAG 
GTCATTATGA CGTCAGGGCA TTCTAAAACA GTAGAAGCAT TTCTTAAAGG TGCTGCTAGA 
AAGAGAAAAT TTCAAGTGAT TGTAGCGGAA TCATCACCCT CTTATCAAGG TCAAAAACTA 
GCTCGTAATT TAGCTGAAGC AAAAATCGAA ACGACCGTAA TTACCGACTC GGCAGTTTTT 
GCTCTGATGT CAAGAGTTAA TAAGGTGATA ATCGGAACTC ATACAGTAAT GGCTGATGGA 
GGATTAAAAG CTTTAAATGG AGCACATGCT TTAACATTAG CAGCAAAGCA TCACTCAGTT 
CCAGTTATTG TTTGTGCTGC CATGTTCAAA CTCTCTCCAA AATTTCTCTG CTCATACGAC 
CAAGATACAT TTAACAAGAT AGTGTCACCT CATGATATAC TGGGATTTAC TGAGGCCGAC 
CTAGTATCCA AGGTTCATGT ACAGAATCCT GTATTCGACT ACGTCCCTCC CGATCTAATT 
AATTTATTTA TTTCTAATAT TGGTGGTAAT GCCCCCTCTT ATGTGTACAG ACTGATCAGT 
GAACTCTATG ATGATGACGA CTCAGATTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100640884 ( XM_003388042.3 ) cDNA ORF clone, Amphimedon queenslandica -> XP_003388090.1 Amphimedon queenslandica translation initiation factor eIF-2B subunit beta-like (LOC100640884), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAm20871
Clone ID Related Accession (Same CDS sequence)	XM_003388042.3
Accession Version	XM_003388042.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-17
Organism	Amphimedon queenslandica
Product	translation initiation factor eIF-2B subunit beta-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003546466.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 18, 2017 this sequence version replaced XM_003388042.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Amphimedon queenslandica Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCG AAGAAGTGGA ACAAAAAGTC CAAATCTTTC TCACATATTT AATTCAAAAG 
AAATTCACGA GTACTTACGA TTTAGCTCAG AAAACGGTCC TACTACTAAG AGATATAATA 
TCAAAATCAA AATGGGCCAA TCCTAGGGAG CTAATGGAGA GGGTGAGGGA GGATGGGAAG 
AGAATCATTA CAGCTCAAGC CGGGGAAAAT GTTGTGTGTA ATATGGTCAG AAGAGTTCTT 
AAAATTATTC GCGAAGAACT TGTAAGGTGT CTGAGAGGAT CTGATTCTGA GAGCATGGAC 
CTCAAGGATT CGTTACATAC TATGCTAAAA GGAGGTGAAG ATTACGATGA TCTATCAAAG 
CCTGTTCCTA ATTTAAAAGG TGCTGTCATT GAAGCCATAC ACGAATTATT AGGAGAAATG 
GAAGAAAGTG CATCCAATAT TGCAGCACAA GCATTGGATC ACATTCATTC CAATGAGGTC 
ATTATGACGT CAGGGCATTC TAAAACAGTA GAAGCATTTC TTAAAGGTGC TGCTAGAAAG 
AGAAAATTTC AAGTGATTGT AGCGGAATCA TCACCCTCTT ATCAAGGTCA AAAACTAGCT 
CGTAATTTAG CTGAAGCAAA AATCGAAACG ACCGTAATTA CCGACTCGGC AGTTTTTGCT 
CTGATGTCAA GAGTTAATAA GGTGATAATC GGAACTCATA CAGTAATGGC TGATGGAGGA 
TTAAAAGCTT TAAATGGAGC ACATGCTTTA ACATTAGCAG CAAAGCATCA CTCAGTTCCA 
GTTATTGTTT GTGCTGCCAT GTTCAAACTC TCTCCAAAAT TTCTCTGCTC ATACGACCAA 
GATACATTTA ACAAGATAGT GTCACCTCAT GATATACTGG GATTTACTGA GGCCGACCTA 
GTATCCAAGG TTCATGTACA GAATCCTGTA TTCGACTACG TCCCTCCCGA TCTAATTAAT 
TTATTTATTT CTAATATTGG TGGTAATGCC CCCTCTTATG TGTACAGACT GATCAGTGAA 
CTCTATGATG ATGACGACTC AGATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003388090.1
CDS	40..1089
Translation	MASEEVEQKVQIFLTYLIQKKFTSTYDLAQKTVLLLRDIISKSKWANPRELMERVREDGKRIITAQAGENVVCNMVRRVLKIIREELVRCLRGSDSESMDLKDSLHTMLKGGEDYDDLSKPVPNLKGAVIEAIHELLGEMEESASNIAAQALDHIHSNEVIMTSGHSKTVEAFLKGAARKRKFQVIVAESSPSYQGQKLARNLAEAKIETTVITDSAVFALMSRVNKVIIGTHTVMADGGLKALNGAHALTLAAKHHSVPVIVCAAMFKLSPKFLCSYDQDTFNKIVSPHDILGFTEADLVSKVHVQNPVFDYVPPDLINLFISNIGGNAPSYVYRLISELYDDDDSDL

Target ORF information:

RefSeq Version	XM_003388042.3
Organism	Amphimedon queenslandica
Definition	Amphimedon queenslandica translation initiation factor eIF-2B subunit beta-like (LOC100640884), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003388042.3

ORF Insert Sequence:

ATGGCGTCCG AAGAAGTGGA ACAAAAAGTC CAAATCTTTC TCACATATTT AATTCAAAAG 
AAATTCACGA GTACTTACGA TTTAGCTCAG AAAACGGTCC TACTACTAAG AGATATAATA 
TCAAAATCAA AATGGGCCAA TCCTAGGGAG CTAATGGAGA GGGTGAGGGA GGATGGGAAG 
AGAATCATTA CAGCTCAAGC CGGGGAAAAT GTTGTGTGTA ATATGGTCAG AAGAGTTCTT 
AAAATTATTC GCGAAGAACT TGTAAGGTGT CTGAGAGGAT CTGATTCTGA GAGCATGGAC 
CTCAAGGATT CGTTACATAC TATGCTAAAA GGAGGTGAAG ATTACGATGA TCTATCAAAG 
CCTGTTCCTA ATTTAAAAGG TGCTGTCATT GAAGCCATAC ACGAATTATT AGGAGAAATG 
GAAGAAAGTG CATCCAATAT TGCAGCACAA GCATTGGATC ACATTCATTC CAATGAGGTC 
ATTATGACGT CAGGGCATTC TAAAACAGTA GAAGCATTTC TTAAAGGTGC TGCTAGAAAG 
AGAAAATTTC AAGTGATTGT AGCGGAATCA TCACCCTCTT ATCAAGGTCA AAAACTAGCT 
CGTAATTTAG CTGAAGCAAA AATCGAAACG ACCGTAATTA CCGACTCGGC AGTTTTTGCT 
CTGATGTCAA GAGTTAATAA GGTGATAATC GGAACTCATA CAGTAATGGC TGATGGAGGA 
TTAAAAGCTT TAAATGGAGC ACATGCTTTA ACATTAGCAG CAAAGCATCA CTCAGTTCCA 
GTTATTGTTT GTGCTGCCAT GTTCAAACTC TCTCCAAAAT TTCTCTGCTC ATACGACCAA 
GATACATTTA ACAAGATAGT GTCACCTCAT GATATACTGG GATTTACTGA GGCCGACCTA 
GTATCCAAGG TTCATGTACA GAATCCTGTA TTCGACTACG TCCCTCCCGA TCTAATTAAT 
TTATTTATTT CTAATATTGG TGGTAATGCC CCCTCTTATG TGTACAGACT GATCAGTGAA 
CTCTATGATG ATGACGACTC AGATTTGTAA