MAP4K5 cDNA ORF clone, Alligator mississippiensis(American alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP4K5
Entrez Gene ID	102563489
Full Name	mitogen-activated protein kinase kinase kinase kinase 5
Gene Type	protein-coding
Organism	Alligator mississippiensis(American alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014595143.2	XP_014450629.2	mitogen-activated protein kinase kinase kinase kinase 5 isoform X1
XM_006263672.3	XP_006263734.3	mitogen-activated protein kinase kinase kinase kinase 5 isoform X2

Pathways from BioSystems
Gene Ontology

The following MAP4K5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP4K5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAl64557	XM_014595143.2 Latest version!	Alligator mississippiensis mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAl64558	XM_006263672.3 Latest version!	Alligator mississippiensis mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP4K5 ( XM_014595143.2 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_014450629.2 Alligator mississippiensis mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAl64557
Clone ID Related Accession (Same CDS sequence)	XM_014595143.2
Accession Version	XM_014595143.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	mitogen-activated protein kinase kinase kinase kinase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017711505.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2016 this sequence version replaced XM_014595143.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGC CGAGCGGGGA CATCATGCGG CGCAGCCCGC AGCAGGACTA CGAGGTGATC 
CAGCGGGTGG GCAGCGGCAC CTACGGCGAC GTCTACAAGG CTAGAAATCT GCATACAGGA 
GAGTTGGCTG CTGTAAAAAT GATCAAGTTA GAGCCAGGTG ATGACTTTTC CTTGATCCAG 
CAAGAAATAT ATATGGTTAA AGAATGCAAG CACTGCAATA TTGTAGCCTA CTTTGGGAGC 
TATCTCAGCC GTGAAAAGCT GTGGATATGT ATGGAATATT GTGGAGGTGG ATCACTTCAA 
GACATATATC ACGTAACAGG ACCGCTGGCA GAGTTACAAA TTGCATATGT CTGCAGAGAA 
ACTTTGCAGG GTCTTTCTTA TTTGCACGTA AAAGGCAAAA TGCATAGAGA TATAAAGGGT 
GCCAATATTC TGCTAACAGA CCATGGTGAT GTGAAGTTAG CTGACTTTGG TGTAGCAGCA 
AAAATAACAG CCACCATTGC GAAGAGAAAA TCTTTTATTG GTACCCCTTA TTGGATGGCT 
CCAGAAGTTG CAGCAGTGGA AAAGAATGGT GGTTACAACC AGCTCTGTGA TATCTGGGCA 
GTTGGCATAA CAGCAATTGA ACTGGCAGAG CTTCAGCCAC CCATGTTTGA TCTTCATCCA 
ATGAGGGCCT TATTTTTAAT GTCAAAAAGC AATTTTCAAC CTCCAAAGCT AAAGGAGAAA 
GCAAAATGGT CATCCACATT CCATAATTTT GTGAAAATAG CACTTACAAA AAATCCAAAG 
AAGAGACCAA CAGCAGACAG GCTCCTTACT CATAGTTTTG TAGGCCAGCC TGGTCTTTCA 
AGATCTCTAG CAGTCGAGCT GTTGGACAAA GTGAATAATC CTGACAACCA CGCACACTAC 
AGTGAAGCTG ATGATGATGA TTTTGAGCCC CACTCTGTTA TTCGCCATAC GATTCGCTCT 
ACAAACAGGA ATGTTAGAGC AGAAAGAACA GCGTCGGAGA TAAATTTTGA CAAGCTGCAG 
TTTGAGCCTC CCTTACGGAA AGAAACAGAA GCTCGGGATG AAATGATTGT GGCAGGAGGT 
GCAGACTTTG CTTCACATTG GAGTCCTTTT GTTGATGGTG GAAGCAGCAG CAAGGGACCG 
CTTACAAAAT TTGGCGATGG CAGTGAAGAT GTTGAAGAAT CAACCCTAAA GCGAGCGGGG 
CCACCTCCAC TACCTCCAAA GCCAAGGATA AACAGTTACC CCGAAGAAAA CCTTCCGGAC 
GATGAGAGAT GTCAGACAAT AAAGCATTTT CCTGACTCCC AAAACAGAGC CCCCTCAGCT 
CACAGGAGAC AGAGTATTCC AGTGTGTGGG CCGCAAACTG AGCCTTCCCC CATCGGTATC 
AGCAGCAGCA TCAGCAGCCC GGGATTACTT ACGACTAGAT ACAGTGACCT GGGAAATGGA 
GGTAGCATGA TGAAACTCAT AGAAGAAAAT ACAGAAGGTT CTGCACAAAT CCCCCAGCTG 
CCACGGAAAA AAGATAAGCG AGACTTTCCG AGGCCAGCGA TCAATGGCCT GCCGCCAACA 
CCAGAGGTGC TGATGGGAGC ATGTTTTTCA AAAGTATTTG ATGGCTGCCC TCTGAAGATT 
AACTGTGCAA CATCATGGAT ACATCCAGAT ACAAAAGATC AATACATTAT TTTTGGAACC 
GAGGAAGGTA TTTATACATT GAATCTGAAC GAACTTCATG AAGCAACAAT GGAGCAGTTA 
TTCCCCCGGA AGTGTACCTG GCTGTATGTT ATCAATAATA CCTTAATGTC ATTATCAGAA 
GGAAAAACGT TTCAGCTTTA CTCCCATAAC TTAATAGCTT TGTTCGAACA AGCCAAAAAA 
ACAGGATTAG CTGCTCATAT TCAAACACAT AGGTTTCCAG ACAAAATATT ACCAAGGAAG 
TTTGCCTTAA CAACAAAGAT TCCCGATACA AAAGGATGCC ACAAATGTTG TATAGTAAGA 
AATCCATATA CAGGACACAA GTACCTGTGT GGGGCATTGC AGTCTGGAAT TGTTTTACTT 
CAGTGGTATG AGCCAATGCA GAAGTTCATG TTAATAAAGC ACTTTGATTT TCCTTTGCCA 
AGCCCTTTGA ATGTGTTTGA AATGCTAGTG ATACCAGAGC AGGAATACCC TATGGTCTGT 
GTAGCTATCA GCAAGGGTAC AGAGCCAAAT CAAGTAGTTC AATTTGAGAC AATCAACTTG 
AACTCTGCTT CTTCGTGGTT TACAGAAATC GGTGCAGGCA ATCAACAGTT GGATGCCATT 
CATGTCACAC AGCTGGAAAG AGACACAGTT CTAGTCTGCT TGGACAAATT CGTGAAAATT 
GTGAATTTAC AAGGGAAGCT GAAGTCAAGC AAGAAGCTGG CCTCTGAGCT AAGTTTTGAT 
TTCTGCATTG AATCTGTGGT GTGCCTCCAG GACAGCGTGT TGGCCTTTTG GAAGCATGGC 
ATGCAAGGCA AAAGTTTTAA ATCAGATGAG GTTACCCAAG AGATATCAGA TGAAACCCGA 
GTTTTCCGCT TGCTGGGATC AGACAGGGTT GTGGTGTTAG AAAGCAGGCC AACAGAAAAT 
CCTACCGCAC ACAGCAATCT CTACATCTTG GCTGGACATG AAAATAGTTA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014450629.2
CDS	44..2677
Translation	MERPSGDIMRRSPQQDYEVIQRVGSGTYGDVYKARNLHTGELAAVKMIKLEPGDDFSLIQQEIYMVKECKHCNIVAYFGSYLSREKLWICMEYCGGGSLQDIYHVTGPLAELQIAYVCRETLQGLSYLHVKGKMHRDIKGANILLTDHGDVKLADFGVAAKITATIAKRKSFIGTPYWMAPEVAAVEKNGGYNQLCDIWAVGITAIELAELQPPMFDLHPMRALFLMSKSNFQPPKLKEKAKWSSTFHNFVKIALTKNPKKRPTADRLLTHSFVGQPGLSRSLAVELLDKVNNPDNHAHYSEADDDDFEPHSVIRHTIRSTNRNVRAERTASEINFDKLQFEPPLRKETEARDEMIVAGGADFASHWSPFVDGGSSSKGPLTKFGDGSEDVEESTLKRAGPPPLPPKPRINSYPEENLPDDERCQTIKHFPDSQNRAPSAHRRQSIPVCGPQTEPSPIGISSSISSPGLLTTRYSDLGNGGSMMKLIEENTEGSAQIPQLPRKKDKRDFPRPAINGLPPTPEVLMGACFSKVFDGCPLKINCATSWIHPDTKDQYIIFGTEEGIYTLNLNELHEATMEQLFPRKCTWLYVINNTLMSLSEGKTFQLYSHNLIALFEQAKKTGLAAHIQTHRFPDKILPRKFALTTKIPDTKGCHKCCIVRNPYTGHKYLCGALQSGIVLLQWYEPMQKFMLIKHFDFPLPSPLNVFEMLVIPEQEYPMVCVAISKGTEPNQVVQFETINLNSASSWFTEIGAGNQQLDAIHVTQLERDTVLVCLDKFVKIVNLQGKLKSSKKLASELSFDFCIESVVCLQDSVLAFWKHGMQGKSFKSDEVTQEISDETRVFRLLGSDRVVVLESRPTENPTAHSNLYILAGHENSY

Target ORF information:

RefSeq Version	XM_014595143.2
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014595143.2

ORF Insert Sequence:

ATGGAGCGGC CGAGCGGGGA CATCATGCGG CGCAGCCCGC AGCAGGACTA CGAGGTGATC 
CAGCGGGTGG GCAGCGGCAC CTACGGCGAC GTCTACAAGG CTAGAAATCT GCATACAGGA 
GAGTTGGCTG CTGTAAAAAT GATCAAGTTA GAGCCAGGTG ATGACTTTTC CTTGATCCAG 
CAAGAAATAT ATATGGTTAA AGAATGCAAG CACTGCAATA TTGTAGCCTA CTTTGGGAGC 
TATCTCAGCC GTGAAAAGCT GTGGATATGT ATGGAATATT GTGGAGGTGG ATCACTTCAA 
GACATATATC ACGTAACAGG ACCGCTGGCA GAGTTACAAA TTGCATATGT CTGCAGAGAA 
ACTTTGCAGG GTCTTTCTTA TTTGCACGTA AAAGGCAAAA TGCATAGAGA TATAAAGGGT 
GCCAATATTC TGCTAACAGA CCATGGTGAT GTGAAGTTAG CTGACTTTGG TGTAGCAGCA 
AAAATAACAG CCACCATTGC GAAGAGAAAA TCTTTTATTG GTACCCCTTA TTGGATGGCT 
CCAGAAGTTG CAGCAGTGGA AAAGAATGGT GGTTACAACC AGCTCTGTGA TATCTGGGCA 
GTTGGCATAA CAGCAATTGA ACTGGCAGAG CTTCAGCCAC CCATGTTTGA TCTTCATCCA 
ATGAGGGCCT TATTTTTAAT GTCAAAAAGC AATTTTCAAC CTCCAAAGCT AAAGGAGAAA 
GCAAAATGGT CATCCACATT CCATAATTTT GTGAAAATAG CACTTACAAA AAATCCAAAG 
AAGAGACCAA CAGCAGACAG GCTCCTTACT CATAGTTTTG TAGGCCAGCC TGGTCTTTCA 
AGATCTCTAG CAGTCGAGCT GTTGGACAAA GTGAATAATC CTGACAACCA CGCACACTAC 
AGTGAAGCTG ATGATGATGA TTTTGAGCCC CACTCTGTTA TTCGCCATAC GATTCGCTCT 
ACAAACAGGA ATGTTAGAGC AGAAAGAACA GCGTCGGAGA TAAATTTTGA CAAGCTGCAG 
TTTGAGCCTC CCTTACGGAA AGAAACAGAA GCTCGGGATG AAATGATTGT GGCAGGAGGT 
GCAGACTTTG CTTCACATTG GAGTCCTTTT GTTGATGGTG GAAGCAGCAG CAAGGGACCG 
CTTACAAAAT TTGGCGATGG CAGTGAAGAT GTTGAAGAAT CAACCCTAAA GCGAGCGGGG 
CCACCTCCAC TACCTCCAAA GCCAAGGATA AACAGTTACC CCGAAGAAAA CCTTCCGGAC 
GATGAGAGAT GTCAGACAAT AAAGCATTTT CCTGACTCCC AAAACAGAGC CCCCTCAGCT 
CACAGGAGAC AGAGTATTCC AGTGTGTGGG CCGCAAACTG AGCCTTCCCC CATCGGTATC 
AGCAGCAGCA TCAGCAGCCC GGGATTACTT ACGACTAGAT ACAGTGACCT GGGAAATGGA 
GGTAGCATGA TGAAACTCAT AGAAGAAAAT ACAGAAGGTT CTGCACAAAT CCCCCAGCTG 
CCACGGAAAA AAGATAAGCG AGACTTTCCG AGGCCAGCGA TCAATGGCCT GCCGCCAACA 
CCAGAGGTGC TGATGGGAGC ATGTTTTTCA AAAGTATTTG ATGGCTGCCC TCTGAAGATT 
AACTGTGCAA CATCATGGAT ACATCCAGAT ACAAAAGATC AATACATTAT TTTTGGAACC 
GAGGAAGGTA TTTATACATT GAATCTGAAC GAACTTCATG AAGCAACAAT GGAGCAGTTA 
TTCCCCCGGA AGTGTACCTG GCTGTATGTT ATCAATAATA CCTTAATGTC ATTATCAGAA 
GGAAAAACGT TTCAGCTTTA CTCCCATAAC TTAATAGCTT TGTTCGAACA AGCCAAAAAA 
ACAGGATTAG CTGCTCATAT TCAAACACAT AGGTTTCCAG ACAAAATATT ACCAAGGAAG 
TTTGCCTTAA CAACAAAGAT TCCCGATACA AAAGGATGCC ACAAATGTTG TATAGTAAGA 
AATCCATATA CAGGACACAA GTACCTGTGT GGGGCATTGC AGTCTGGAAT TGTTTTACTT 
CAGTGGTATG AGCCAATGCA GAAGTTCATG TTAATAAAGC ACTTTGATTT TCCTTTGCCA 
AGCCCTTTGA ATGTGTTTGA AATGCTAGTG ATACCAGAGC AGGAATACCC TATGGTCTGT 
GTAGCTATCA GCAAGGGTAC AGAGCCAAAT CAAGTAGTTC AATTTGAGAC AATCAACTTG 
AACTCTGCTT CTTCGTGGTT TACAGAAATC GGTGCAGGCA ATCAACAGTT GGATGCCATT 
CATGTCACAC AGCTGGAAAG AGACACAGTT CTAGTCTGCT TGGACAAATT CGTGAAAATT 
GTGAATTTAC AAGGGAAGCT GAAGTCAAGC AAGAAGCTGG CCTCTGAGCT AAGTTTTGAT 
TTCTGCATTG AATCTGTGGT GTGCCTCCAG GACAGCGTGT TGGCCTTTTG GAAGCATGGC 
ATGCAAGGCA AAAGTTTTAA ATCAGATGAG GTTACCCAAG AGATATCAGA TGAAACCCGA 
GTTTTCCGCT TGCTGGGATC AGACAGGGTT GTGGTGTTAG AAAGCAGGCC AACAGAAAAT 
CCTACCGCAC ACAGCAATCT CTACATCTTG GCTGGACATG AAAATAGTTA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K5 ( XM_006263672.3 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_006263734.3 Alligator mississippiensis mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAl64558
Clone ID Related Accession (Same CDS sequence)	XM_006263672.3
Accession Version	XM_006263672.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	mitogen-activated protein kinase kinase kinase kinase 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017711505.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2016 this sequence version replaced XM_006263672.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGC CGAGCGGGGA CATCATGCGG CGCAGCCCGC AGCAGGACTA CGAGGTGATC 
CAGCGGGTGG GCAGCGGCAC CTACGGCGAC GTCTACAAGG CTAGAAATCT GCATACAGGA 
GAGTTGGCTG CTGTAAAAAT GATCAAGTTA GAGCCAGGTG ATGACTTTTC CTTGATCCAG 
CAAGAAATAT ATATGGTTAA AGAATGCAAG CACTGCAATA TTGTAGCCTA CTTTGGGAGC 
TATCTCAGCC GTGAAAAGCT GTGGATATGT ATGGAATATT GTGGAGGTGG ATCACTTCAA 
GACATATATC ACGTAACAGG ACCGCTGGCA GAGTTACAAA TTGCATATGT CTGCAGAGAA 
ACTTTGCAGG GTCTTTCTTA TTTGCACGTA AAAGGCAAAA TGCATAGAGA TATAAAGGGT 
GCCAATATTC TGCTAACAGA CCATGGTGAT GTGAAGTTAG CTGACTTTGG TGTAGCAGCA 
AAAATAACAG CCACCATTGC GAAGAGAAAA TCTTTTATTG GTACCCCTTA TTGGATGGCT 
CCAGAAGTTG CAGCAGTGGA AAAGAATGGT GGTTACAACC AGCTCTGTGA TATCTGGGCA 
GTTGGCATAA CAGCAATTGA ACTGGCAGAG CTTCAGCCAC CCATGTTTGA TCTTCATCCA 
ATGAGGGCCT TATTTTTAAT GTCAAAAAGC AATTTTCAAC CTCCAAAGCT AAAGGAGAAA 
GCAAAATGGT CATCCACATT CCATAATTTT GTGAAAATAG CACTTACAAA AAATCCAAAG 
AAGAGACCAA CAGCAGACAG GCTCCTTACT CATAGTTTTG TAGGCCAGCC TGGTCTTTCA 
AGATCTCTAG CAGTCGAGCT GTTGGACAAA GTGAATAATC CTGACAACCA CGCACACTAC 
AGTGAAGCTG ATGATGATGA TTTTGAGCCC CACTCTGTTA TTCGCCATAC GATTCGCTCT 
ACAAACAGGA ATGTTAGAGC AGAAAGAACA GCGTCGGAGA TAAATTTTGA CAAGCTGCAG 
TTTGAGCCTC CCTTACGGAA AGAAACAGAA GCTCGGGATG AAATGATTGT GGCAGGAGGT 
GCAGACTTTG CTTCACATTG GAGTCCTTTT GTTGATGGTG GAAGCAGCAG CAAATCAACC 
CTAAAGCGAG CGGGGCCACC TCCACTACCT CCAAAGCCAA GGATAAACAG TTACCCCGAA 
GAAAACCTTC CGGACGATGA GAGATGTCAG ACAATAAAGC ATTTTCCTGA CTCCCAAAAC 
AGAGCCCCCT CAGCTCACAG GAGACAGAGT ATTCCAGTGT GTGGGCCGCA AACTGAGCCT 
TCCCCCATCG GTATCAGCAG CAGCATCAGC AGCCCGGGAT TACTTACGAC TAGATACAGT 
GACCTGGGAA ATGGAGGTAG CATGATGAAA CTCATAGAAG AAAATACAGA AGGTTCTGCA 
CAAATCCCCC AGCTGCCACG GAAAAAAGAT AAGCGAGACT TTCCGAGGCC AGCGATCAAT 
GGCCTGCCGC CAACACCAGA GGTGCTGATG GGAGCATGTT TTTCAAAAGT ATTTGATGGC 
TGCCCTCTGA AGATTAACTG TGCAACATCA TGGATACATC CAGATACAAA AGATCAATAC 
ATTATTTTTG GAACCGAGGA AGGTATTTAT ACATTGAATC TGAACGAACT TCATGAAGCA 
ACAATGGAGC AGTTATTCCC CCGGAAGTGT ACCTGGCTGT ATGTTATCAA TAATACCTTA 
ATGTCATTAT CAGAAGGAAA AACGTTTCAG CTTTACTCCC ATAACTTAAT AGCTTTGTTC 
GAACAAGCCA AAAAAACAGG ATTAGCTGCT CATATTCAAA CACATAGGTT TCCAGACAAA 
ATATTACCAA GGAAGTTTGC CTTAACAACA AAGATTCCCG ATACAAAAGG ATGCCACAAA 
TGTTGTATAG TAAGAAATCC ATATACAGGA CACAAGTACC TGTGTGGGGC ATTGCAGTCT 
GGAATTGTTT TACTTCAGTG GTATGAGCCA ATGCAGAAGT TCATGTTAAT AAAGCACTTT 
GATTTTCCTT TGCCAAGCCC TTTGAATGTG TTTGAAATGC TAGTGATACC AGAGCAGGAA 
TACCCTATGG TCTGTGTAGC TATCAGCAAG GGTACAGAGC CAAATCAAGT AGTTCAATTT 
GAGACAATCA ACTTGAACTC TGCTTCTTCG TGGTTTACAG AAATCGGTGC AGGCAATCAA 
CAGTTGGATG CCATTCATGT CACACAGCTG GAAAGAGACA CAGTTCTAGT CTGCTTGGAC 
AAATTCGTGA AAATTGTGAA TTTACAAGGG AAGCTGAAGT CAAGCAAGAA GCTGGCCTCT 
GAGCTAAGTT TTGATTTCTG CATTGAATCT GTGGTGTGCC TCCAGGACAG CGTGTTGGCC 
TTTTGGAAGC ATGGCATGCA AGGCAAAAGT TTTAAATCAG ATGAGGTTAC CCAAGAGATA 
TCAGATGAAA CCCGAGTTTT CCGCTTGCTG GGATCAGACA GGGTTGTGGT GTTAGAAAGC 
AGGCCAACAG AAAATCCTAC CGCACACAGC AATCTCTACA TCTTGGCTGG ACATGAAAAT 
AGTTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006263734.3
CDS	44..2632
Translation	MERPSGDIMRRSPQQDYEVIQRVGSGTYGDVYKARNLHTGELAAVKMIKLEPGDDFSLIQQEIYMVKECKHCNIVAYFGSYLSREKLWICMEYCGGGSLQDIYHVTGPLAELQIAYVCRETLQGLSYLHVKGKMHRDIKGANILLTDHGDVKLADFGVAAKITATIAKRKSFIGTPYWMAPEVAAVEKNGGYNQLCDIWAVGITAIELAELQPPMFDLHPMRALFLMSKSNFQPPKLKEKAKWSSTFHNFVKIALTKNPKKRPTADRLLTHSFVGQPGLSRSLAVELLDKVNNPDNHAHYSEADDDDFEPHSVIRHTIRSTNRNVRAERTASEINFDKLQFEPPLRKETEARDEMIVAGGADFASHWSPFVDGGSSSKSTLKRAGPPPLPPKPRINSYPEENLPDDERCQTIKHFPDSQNRAPSAHRRQSIPVCGPQTEPSPIGISSSISSPGLLTTRYSDLGNGGSMMKLIEENTEGSAQIPQLPRKKDKRDFPRPAINGLPPTPEVLMGACFSKVFDGCPLKINCATSWIHPDTKDQYIIFGTEEGIYTLNLNELHEATMEQLFPRKCTWLYVINNTLMSLSEGKTFQLYSHNLIALFEQAKKTGLAAHIQTHRFPDKILPRKFALTTKIPDTKGCHKCCIVRNPYTGHKYLCGALQSGIVLLQWYEPMQKFMLIKHFDFPLPSPLNVFEMLVIPEQEYPMVCVAISKGTEPNQVVQFETINLNSASSWFTEIGAGNQQLDAIHVTQLERDTVLVCLDKFVKIVNLQGKLKSSKKLASELSFDFCIESVVCLQDSVLAFWKHGMQGKSFKSDEVTQEISDETRVFRLLGSDRVVVLESRPTENPTAHSNLYILAGHENSY

Target ORF information:

RefSeq Version	XM_006263672.3
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006263672.3

ORF Insert Sequence:

ATGGAGCGGC CGAGCGGGGA CATCATGCGG CGCAGCCCGC AGCAGGACTA CGAGGTGATC 
CAGCGGGTGG GCAGCGGCAC CTACGGCGAC GTCTACAAGG CTAGAAATCT GCATACAGGA 
GAGTTGGCTG CTGTAAAAAT GATCAAGTTA GAGCCAGGTG ATGACTTTTC CTTGATCCAG 
CAAGAAATAT ATATGGTTAA AGAATGCAAG CACTGCAATA TTGTAGCCTA CTTTGGGAGC 
TATCTCAGCC GTGAAAAGCT GTGGATATGT ATGGAATATT GTGGAGGTGG ATCACTTCAA 
GACATATATC ACGTAACAGG ACCGCTGGCA GAGTTACAAA TTGCATATGT CTGCAGAGAA 
ACTTTGCAGG GTCTTTCTTA TTTGCACGTA AAAGGCAAAA TGCATAGAGA TATAAAGGGT 
GCCAATATTC TGCTAACAGA CCATGGTGAT GTGAAGTTAG CTGACTTTGG TGTAGCAGCA 
AAAATAACAG CCACCATTGC GAAGAGAAAA TCTTTTATTG GTACCCCTTA TTGGATGGCT 
CCAGAAGTTG CAGCAGTGGA AAAGAATGGT GGTTACAACC AGCTCTGTGA TATCTGGGCA 
GTTGGCATAA CAGCAATTGA ACTGGCAGAG CTTCAGCCAC CCATGTTTGA TCTTCATCCA 
ATGAGGGCCT TATTTTTAAT GTCAAAAAGC AATTTTCAAC CTCCAAAGCT AAAGGAGAAA 
GCAAAATGGT CATCCACATT CCATAATTTT GTGAAAATAG CACTTACAAA AAATCCAAAG 
AAGAGACCAA CAGCAGACAG GCTCCTTACT CATAGTTTTG TAGGCCAGCC TGGTCTTTCA 
AGATCTCTAG CAGTCGAGCT GTTGGACAAA GTGAATAATC CTGACAACCA CGCACACTAC 
AGTGAAGCTG ATGATGATGA TTTTGAGCCC CACTCTGTTA TTCGCCATAC GATTCGCTCT 
ACAAACAGGA ATGTTAGAGC AGAAAGAACA GCGTCGGAGA TAAATTTTGA CAAGCTGCAG 
TTTGAGCCTC CCTTACGGAA AGAAACAGAA GCTCGGGATG AAATGATTGT GGCAGGAGGT 
GCAGACTTTG CTTCACATTG GAGTCCTTTT GTTGATGGTG GAAGCAGCAG CAAATCAACC 
CTAAAGCGAG CGGGGCCACC TCCACTACCT CCAAAGCCAA GGATAAACAG TTACCCCGAA 
GAAAACCTTC CGGACGATGA GAGATGTCAG ACAATAAAGC ATTTTCCTGA CTCCCAAAAC 
AGAGCCCCCT CAGCTCACAG GAGACAGAGT ATTCCAGTGT GTGGGCCGCA AACTGAGCCT 
TCCCCCATCG GTATCAGCAG CAGCATCAGC AGCCCGGGAT TACTTACGAC TAGATACAGT 
GACCTGGGAA ATGGAGGTAG CATGATGAAA CTCATAGAAG AAAATACAGA AGGTTCTGCA 
CAAATCCCCC AGCTGCCACG GAAAAAAGAT AAGCGAGACT TTCCGAGGCC AGCGATCAAT 
GGCCTGCCGC CAACACCAGA GGTGCTGATG GGAGCATGTT TTTCAAAAGT ATTTGATGGC 
TGCCCTCTGA AGATTAACTG TGCAACATCA TGGATACATC CAGATACAAA AGATCAATAC 
ATTATTTTTG GAACCGAGGA AGGTATTTAT ACATTGAATC TGAACGAACT TCATGAAGCA 
ACAATGGAGC AGTTATTCCC CCGGAAGTGT ACCTGGCTGT ATGTTATCAA TAATACCTTA 
ATGTCATTAT CAGAAGGAAA AACGTTTCAG CTTTACTCCC ATAACTTAAT AGCTTTGTTC 
GAACAAGCCA AAAAAACAGG ATTAGCTGCT CATATTCAAA CACATAGGTT TCCAGACAAA 
ATATTACCAA GGAAGTTTGC CTTAACAACA AAGATTCCCG ATACAAAAGG ATGCCACAAA 
TGTTGTATAG TAAGAAATCC ATATACAGGA CACAAGTACC TGTGTGGGGC ATTGCAGTCT 
GGAATTGTTT TACTTCAGTG GTATGAGCCA ATGCAGAAGT TCATGTTAAT AAAGCACTTT 
GATTTTCCTT TGCCAAGCCC TTTGAATGTG TTTGAAATGC TAGTGATACC AGAGCAGGAA 
TACCCTATGG TCTGTGTAGC TATCAGCAAG GGTACAGAGC CAAATCAAGT AGTTCAATTT 
GAGACAATCA ACTTGAACTC TGCTTCTTCG TGGTTTACAG AAATCGGTGC AGGCAATCAA 
CAGTTGGATG CCATTCATGT CACACAGCTG GAAAGAGACA CAGTTCTAGT CTGCTTGGAC 
AAATTCGTGA AAATTGTGAA TTTACAAGGG AAGCTGAAGT CAAGCAAGAA GCTGGCCTCT 
GAGCTAAGTT TTGATTTCTG CATTGAATCT GTGGTGTGCC TCCAGGACAG CGTGTTGGCC 
TTTTGGAAGC ATGGCATGCA AGGCAAAAGT TTTAAATCAG ATGAGGTTAC CCAAGAGATA 
TCAGATGAAA CCCGAGTTTT CCGCTTGCTG GGATCAGACA GGGTTGTGGT GTTAGAAAGC 
AGGCCAACAG AAAATCCTAC CGCACACAGC AATCTCTACA TCTTGGCTGG ACATGAAAAT 
AGTTACTAA