RAPGEF6 cDNA ORF clone, Alligator mississippiensis(American alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAPGEF6
Entrez Gene ID	102557740
Full Name	Rap guanine nucleotide exchange factor 6
Synonyms	RAPGEF2
Gene Type	protein-coding
Organism	Alligator mississippiensis(American alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019478368.1	XP_019333913.1	rap guanine nucleotide exchange factor 6 isoform X7
XM_019478364.1	XP_019333909.1	rap guanine nucleotide exchange factor 6 isoform X3
XM_019478369.1	XP_019333914.1	rap guanine nucleotide exchange factor 6 isoform X8
XM_014597587.2	XP_014453073.1	rap guanine nucleotide exchange factor 6 isoform X1
XM_019478363.1	XP_019333908.1	rap guanine nucleotide exchange factor 6 isoform X2
XM_019478367.1	XP_019333912.1	rap guanine nucleotide exchange factor 6 isoform X6
XM_014597588.2	XP_014453074.1	rap guanine nucleotide exchange factor 6 isoform X4
XM_019478366.1	XP_019333911.1	rap guanine nucleotide exchange factor 6 isoform X5

Pathways from BioSystems
Gene Ontology

The following RAPGEF6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAl76325	XM_014597587.2 Latest version!	Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAl76326	XM_019478363.1 Latest version!	Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAl76327	XM_019478364.1 Latest version!	Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAl76328	XM_014597588.2 Latest version!	Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAl76329	XM_019478366.1 Latest version!	Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAl76330	XM_019478367.1 Latest version!	Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAl76331	XM_019478368.1 Latest version!	Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAl76332	XM_019478369.1 Latest version!	Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAPGEF6 ( XM_014597587.2 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_014453073.1 Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAl76325
Clone ID Related Accession (Same CDS sequence)	XM_014597587.2
Accession Version	XM_014597587.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	rap guanine nucleotide exchange factor 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017713851.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2016 this sequence version replaced XM_014597587.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCCG CCGTGGACCC CAAGGTCCAA CAGGCCCTGC GGAAGAAGCC CCCGGAGCGG 
AGCCGCGAGG ACTTAAATAT TATCTATACT TATCTTCATG GAATGGACAT ATTGTCTCAT 
CTCAGGGAAC ATCAGCTAAG AATAATGTCT TCAAGTGCCC GCTATGAAAG ATACAAGGGA 
AACCAAGTTC TCTTTTGCTC AGAAACAATC GCCAGATGTT GGTACATTCT TCTTTCCGGA 
TCTGTGCTTA TGAAGGACTC CATGTTTTTA CCACCATGCA GTTTTGGCAA GCAGTCTGGA 
GGAAAGCGAG GATGCGAGTG TATTGTACTG GAGCCTTCTG AAATGATTGT GGTGGAGAAC 
TCCAAAGACA ACGAAGAAAA TATCTTGCAA AGAGAAGTTC CCATCAGACA GTCTCGGCGA 
AGGTTCAGGA AGATTAATCA ACGGGGAGAA CGCCAGACCA TCACTGACCA TGTGGATGCT 
AGCAGTTATC TTTCGCTTCC AGCAGATCTT ACCAAGATGC AAATTGCAGA CAATCCACAT 
CCACAGGTGA CTCATGTCCC TTCCAGCCAG TCTGGATGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGCT TGTCTGATAT ATATCAGGCT ACAGAAAGTG AGATGGGAGA TGTAGATTTA 
ACAGGCCTCC CAGAAGCACC TGTGGACTCT GAAGATGAAG AGGAGGAGGA AGATGAAGAT 
ATAGACAGAA CTTCAGATCC ATTATTGGGG CGAGACGTTG TACGAGAGTG CCTTGAAAAA 
GAACCTGCAG ATAAAACGGA TGATGACATT GAGCAATTAC TGGAATTTAT GCATCAGCTC 
CCAGCTTTTG CAAACATGAC CATGTCTGTG AGAAGAGAGC TTTGTTCAGT TATGATATTT 
GAAGTGGTAG AACAAGCAGG AGCCATCATA CTTGAAGACG GACAAGAACT TGATTCTTGG 
TATGTTATTT TAAATGGCAC TGTGGAGATC AGCTATCCAG ATGGGAAAAC TGAAAATCTC 
TGTATGGGAA ATAGTTTTGG GATTACTCCT TCTCTGGAAA AACAGTATAT GAATGGAGTG 
GTTAGAACCA AAGTAGATGA CTGTCAGTTT GTATGCATAG CCCAGCAAGA TTATTGGCGC 
ATTCTGAACC ACGTGGAAAA AAACACTCAT AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAGGAGC ACAGAGAGCT GGATCGCAGT GGAACCAGGA AAGGACACAT AGTTATTAAG 
GCAACACCTG AGCGACTCAT CACACATTTA ATAGAAGAGC ATTCCATCGT TGATCCAACA 
TACATAGAAG ACTTCCTTTT AACATATAGA ACATTTCTCT CGAGCCCCTT GGAAGTTGGG 
GATAAGCTCT TGGAGTGGTT TAAAATAGAT AGCCTCAGGG ATAAGGTTAC CAGAGTGGTC 
TTATTGTGGG TAAACAATCA TTTTAATGAT TTTGAAGGAG ATCCTGCTAT GACACGTTTT 
CTGGAAGAAT TTGAAAAAAA CTTGGAAAAT ACGAAAATGA AGGGGCACCT CAGGTTGTTG 
AATATTGCTT GTGCTGCCAA GGCAAAATGG AGGCAGATTA CCCTGCAGAA GGCTTCCAGA 
GACTCGCCTC TGTATTTCAG CCTCCTGGGA GGAAGTGAGA AGGGTTTTGG TATATTTGTA 
GAGACTGTAG AAACAGGCAG CAAAGCAGCA GAAGTTGGGC TGAAGCGTGG CGATCAGATA 
ATGGAAGTAA ATGGGCAGAA CTTTGAGAAC ATTACTTTTG TGAAAGCCCT TGAAATCTTG 
AGGAACAATA CTCATCTTTC CATTACAGTA AAGACAAACA TTTTTGTGTT TAAGGAACTG 
CTTAGCCGGA TAGGCCAGGA GAAAAACGGA GTCCCACATA TTCCAAAAAT TGCAGAAAAG 
AAAAATAACC GTTACTCCAT CCCAGATATG CCAGGAGACA TGGAACAGAT ATTTCCACGT 
GAGAAAGGAA ACAAGAAAAT GAAAGCAAAC ACTGTATCCG GTGGAAGAAA TAGGATCAAG 
AAAATTTTAG ACAAGACTCG TTTCAGCATT TTGCCCCCAA AACTATTTAG TGATGGCAGT 
GTAAGTCAAT CCCAGGATGA CAGCATTGTT GGAACAAGGC AGTGCAGACA CAGCCTGGCC 
ATCATGCCTG TCCCTGGAAC ACTTTCATCC AGCAGCCCGG ACCTTTTGCA GCCTACAACC 
AGCATTCTTG ATTTTTCTAA TCCTTCAGCG GTCGGTTTTT ACTATATTCC AGATCAAGTT 
ATTAGAGTTT TCAAAGCAGA TCAACAGAGT TGTTACATTA TCATCAGCAA AGACACTACA 
GCGAAAGAAG TTGTGAGTCA TGCTGTGCAT GAATTCAACT TGACGGGTGC GTCAGAGACC 
TACTCTCTCT GCGAGGTGTC TGTTAGTCCT GAGGGAGTCA TCAAACAAAG AAGGCTTCCA 
GATCAGTTCT CAAAATTAGC AGACAGGATT CAGCTCAATG GAAGGTATTA TCTGAAAAAC 
AATATGGAAA CAGAGACCTT ATGCTCAGAT GAAGATGCCC AAGAGCTGCT CAGAGAGAGT 
CAAATATCTC TTCTTCAGCT GAGCACCATT GAAGTGGCAA CTCAGCTTTC AATGAGAGAC 
TTTGAATTAT TTCGCAACAT TGAGCCTACT GAATACATTG ATGACCTTTT TAAGCTGGAC 
TCAAAAACAG GAAATGCTCA CCTGAAACAA TTTGAGGATG TCATTAACCA AGAGACATTT 
TGGGTTGCCA CAGAAATTTT ATTAGAGCCC AACCAGCTCA AAAGAATGAA AATTATTAAA 
CATTTCATTA AAATTGCCCT CCACTGTCGA GAATGCAAGA ACTTCAATTC CATGTTTGCA 
GTTATAAGTG GGCTAAATCT GGCATCGGTA GCACGACTCA GAGGTACCTG GGAAAAATTA 
CCTAGCAAGT ATGAAAAGCA TTTCAGAGAC CTTCAGGATC TTTTTGATCC ATCTAGAAAC 
ATGGCAAAAT ACCGCAATAT TCTTAGCAGT CAGAGCATGC AGCCTCCAAT TATTCCACTC 
TTTCCCGTTG TCAAGAAGGA TATTACATTT CTACATGAAG GAAATGATTC CAAAGTGGAT 
GGCCTGGTAA ATTTTGAGAA ACTCAGAATG ATTGCCAAAG AAATCCGGCA AGTTATCAGA 
ATGACCTCTG CTAACATGGA TCCGGCTGTA ATGTTCAGGC AAAGGAAGAA GAGGTGGAGG 
AGTTTGGGGT CTCTGAGCCA GGGCAGCACA AACTCAAATA TGCTCGATGT TCAAGGAGGT 
GCTCACAAAA AACGTGCCCG ACGCAGTTCG CTCCTTAATG CCAAGAAGCT ATATGAGGAT 
GCCCAGATGG CCAGAAAGGT GAAGCAGTAC CTGTCCAACC TGAATGTAGA AACTGATGAG 
GAAAAATTCC AGATCATGTC ACTTCAGTGC GAGCCTGCTT ATAGCACTTT GACAAAGAAT 
TTAAGTGAGA GAAGAGGTGG GAAATCCTCA GAAATGTCTC CAATACCCTT GAGGTCAGTT 
GGCCAAACCA CAAAAGTTCA GCAGCATCAG CAAAACAGAA TGAGCCAAGT TCTTCAAGTT 
CCTGCTGTTA ATTTGTATCC CATTAGAAAA AAGGGACCAG CAAAGGACCA CGTCACTTTT 
AATACAGGTT CTCCTCAGAA GTCATTAAGT TTGTCGGAGG AAGTAAGTAA GAAACCAGCA 
GAAGATGCTG TGTCTATGGC ATCTTCTTTG CACTCCAGTC CTCTTGCATC TCCTCAGGGC 
TCTCCACGCA AAGTTGGTAA CATCCAGAGG TCTGATAATT GCAGTCAGAT GAATCTTTCT 
GGATCCTCTT CATCGCTTGC CAGTGAAACC AGTAACAAGA ACAGTGGGCA ACGCAGCTAT 
GGAATAGGCT ATGCTCTCAT ACCTTCTGCC AAGTCAGATA ATTTCTCAGA CTCCAGCCAC 
AGCGAGATCT CCTCACGGTC CAGCATAGTG AGCAACTGCT CTGTCGACTC CATGTCTGCA 
GCCCTCCAGG ACGAGAGGGG TTTGCCCCAG TCTCTTAGTG TCGTGGATTC AGTGGGAACA 
GAAAGAAAAG AACATCCTCC ACCATTGGCT GAGTACAGTC AGCCTTGCCC AGGTTGGCCC 
CTCCCTAGAC CGGCTATAAT CAGAGGATTG GCTGTCACAT CGTCCATGAG CAATGAGGAG 
ATCTCCCATG AACATATTAC AATAGAAGCC GCAGACAGTG GCCGTGGAAG CTGGACTTCA 
TGTTCAAGTA GTTCTCACGA TAACTTCCAG AATATCCAAA TTCAGAAAAG CTGGGATCTC 
TTGAATTCTT ACCGCCACAC TCACTTGGAT GGACCTATTG CTGAAGTAGA ACCCACAAAC 
TGTTACCCTG AAGAGGCGTA TTTGTATTCC GAAGCCTTTT CCAAAAATAA TAGGCAGTCC 
AAAACCAGCG TGGAATTGGA TCAGTCTCGG CAGAGCTGGG CCTCCTCTAG CTCTCTGTCA 
GACACGTATG AAACAAACTA TGGGACGATT AAACGGAGAG GGCTGGAGAA CTCAACAATG 
GAGCAGACTG AGATTTTGGA CTCTAAACCA GGCACTGATG CAACTTACAA AACTGTTACT 
TCTAGTACAG AAAAGGGCCT AATAGTGTAC TGTGTCACCT CACCTAAGAA GGACGATAGG 
TATAGGGAGC CACCGCCCAC CCCTCCGGGA TATATGGGGA TTTCTTTAGC GGACATAAAG 
GAAGGATCGC CCCACCACCC ACACTTAAAA CCTCCAGACT ATAGTGTGGC AGTGCAGAGG 
TCAAAGATGC TACATAGTGA CCTCTCTAGA CTTTCATCAG CTTCTCTCAG TAGTGAACCA 
GTGGCCTGTA TTTCATCGAA GATGCCTCAG TGGCATACCC GGCAGCCGTC CCATCCTAAA 
CTAACAGATA TGACTGACGC AGATAGTGAA GAGGATGAAG ATGAACAAGT ATCAGCAGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014453073.1
CDS	268..5250
Translation	MGSAVDPKVQQALRKKPPERSREDLNIIYTYLHGMDILSHLREHQLRIMSSSARYERYKGNQVLFCSETIARCWYILLSGSVLMKDSMFLPPCSFGKQSGGKRGCECIVLEPSEMIVVENSKDNEENILQREVPIRQSRRRFRKINQRGERQTITDHVDASSYLSLPADLTKMQIADNPHPQVTHVPSSQSGCSIASDSGSSSLSDIYQATESEMGDVDLTGLPEAPVDSEDEEEEEDEDIDRTSDPLLGRDVVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISYPDGKTENLCMGNSFGITPSLEKQYMNGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVKEHRELDRSGTRKGHIVIKATPERLITHLIEEHSIVDPTYIEDFLLTYRTFLSSPLEVGDKLLEWFKIDSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFEKNLENTKMKGHLRLLNIACAAKAKWRQITLQKASRDSPLYFSLLGGSEKGFGIFVETVETGSKAAEVGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLSITVKTNIFVFKELLSRIGQEKNGVPHIPKIAEKKNNRYSIPDMPGDMEQIFPREKGNKKMKANTVSGGRNRIKKILDKTRFSILPPKLFSDGSVSQSQDDSIVGTRQCRHSLAIMPVPGTLSSSSPDLLQPTTSILDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVSHAVHEFNLTGASETYSLCEVSVSPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTIEVATQLSMRDFELFRNIEPTEYIDDLFKLDSKTGNAHLKQFEDVINQETFWVATEILLEPNQLKRMKIIKHFIKIALHCRECKNFNSMFAVISGLNLASVARLRGTWEKLPSKYEKHFRDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDITFLHEGNDSKVDGLVNFEKLRMIAKEIRQVIRMTSANMDPAVMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSNLNVETDEEKFQIMSLQCEPAYSTLTKNLSERRGGKSSEMSPIPLRSVGQTTKVQQHQQNRMSQVLQVPAVNLYPIRKKGPAKDHVTFNTGSPQKSLSLSEEVSKKPAEDAVSMASSLHSSPLASPQGSPRKVGNIQRSDNCSQMNLSGSSSSLASETSNKNSGQRSYGIGYALIPSAKSDNFSDSSHSEISSRSSIVSNCSVDSMSAALQDERGLPQSLSVVDSVGTERKEHPPPLAEYSQPCPGWPLPRPAIIRGLAVTSSMSNEEISHEHITIEAADSGRGSWTSCSSSSHDNFQNIQIQKSWDLLNSYRHTHLDGPIAEVEPTNCYPEEAYLYSEAFSKNNRQSKTSVELDQSRQSWASSSSLSDTYETNYGTIKRRGLENSTMEQTEILDSKPGTDATYKTVTSSTEKGLIVYCVTSPKKDDRYREPPPTPPGYMGISLADIKEGSPHHPHLKPPDYSVAVQRSKMLHSDLSRLSSASLSSEPVACISSKMPQWHTRQPSHPKLTDMTDADSEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014597587.2
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014597587.2

ORF Insert Sequence:

ATGGGCTCCG CCGTGGACCC CAAGGTCCAA CAGGCCCTGC GGAAGAAGCC CCCGGAGCGG 
AGCCGCGAGG ACTTAAATAT TATCTATACT TATCTTCATG GAATGGACAT ATTGTCTCAT 
CTCAGGGAAC ATCAGCTAAG AATAATGTCT TCAAGTGCCC GCTATGAAAG ATACAAGGGA 
AACCAAGTTC TCTTTTGCTC AGAAACAATC GCCAGATGTT GGTACATTCT TCTTTCCGGA 
TCTGTGCTTA TGAAGGACTC CATGTTTTTA CCACCATGCA GTTTTGGCAA GCAGTCTGGA 
GGAAAGCGAG GATGCGAGTG TATTGTACTG GAGCCTTCTG AAATGATTGT GGTGGAGAAC 
TCCAAAGACA ACGAAGAAAA TATCTTGCAA AGAGAAGTTC CCATCAGACA GTCTCGGCGA 
AGGTTCAGGA AGATTAATCA ACGGGGAGAA CGCCAGACCA TCACTGACCA TGTGGATGCT 
AGCAGTTATC TTTCGCTTCC AGCAGATCTT ACCAAGATGC AAATTGCAGA CAATCCACAT 
CCACAGGTGA CTCATGTCCC TTCCAGCCAG TCTGGATGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGCT TGTCTGATAT ATATCAGGCT ACAGAAAGTG AGATGGGAGA TGTAGATTTA 
ACAGGCCTCC CAGAAGCACC TGTGGACTCT GAAGATGAAG AGGAGGAGGA AGATGAAGAT 
ATAGACAGAA CTTCAGATCC ATTATTGGGG CGAGACGTTG TACGAGAGTG CCTTGAAAAA 
GAACCTGCAG ATAAAACGGA TGATGACATT GAGCAATTAC TGGAATTTAT GCATCAGCTC 
CCAGCTTTTG CAAACATGAC CATGTCTGTG AGAAGAGAGC TTTGTTCAGT TATGATATTT 
GAAGTGGTAG AACAAGCAGG AGCCATCATA CTTGAAGACG GACAAGAACT TGATTCTTGG 
TATGTTATTT TAAATGGCAC TGTGGAGATC AGCTATCCAG ATGGGAAAAC TGAAAATCTC 
TGTATGGGAA ATAGTTTTGG GATTACTCCT TCTCTGGAAA AACAGTATAT GAATGGAGTG 
GTTAGAACCA AAGTAGATGA CTGTCAGTTT GTATGCATAG CCCAGCAAGA TTATTGGCGC 
ATTCTGAACC ACGTGGAAAA AAACACTCAT AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAGGAGC ACAGAGAGCT GGATCGCAGT GGAACCAGGA AAGGACACAT AGTTATTAAG 
GCAACACCTG AGCGACTCAT CACACATTTA ATAGAAGAGC ATTCCATCGT TGATCCAACA 
TACATAGAAG ACTTCCTTTT AACATATAGA ACATTTCTCT CGAGCCCCTT GGAAGTTGGG 
GATAAGCTCT TGGAGTGGTT TAAAATAGAT AGCCTCAGGG ATAAGGTTAC CAGAGTGGTC 
TTATTGTGGG TAAACAATCA TTTTAATGAT TTTGAAGGAG ATCCTGCTAT GACACGTTTT 
CTGGAAGAAT TTGAAAAAAA CTTGGAAAAT ACGAAAATGA AGGGGCACCT CAGGTTGTTG 
AATATTGCTT GTGCTGCCAA GGCAAAATGG AGGCAGATTA CCCTGCAGAA GGCTTCCAGA 
GACTCGCCTC TGTATTTCAG CCTCCTGGGA GGAAGTGAGA AGGGTTTTGG TATATTTGTA 
GAGACTGTAG AAACAGGCAG CAAAGCAGCA GAAGTTGGGC TGAAGCGTGG CGATCAGATA 
ATGGAAGTAA ATGGGCAGAA CTTTGAGAAC ATTACTTTTG TGAAAGCCCT TGAAATCTTG 
AGGAACAATA CTCATCTTTC CATTACAGTA AAGACAAACA TTTTTGTGTT TAAGGAACTG 
CTTAGCCGGA TAGGCCAGGA GAAAAACGGA GTCCCACATA TTCCAAAAAT TGCAGAAAAG 
AAAAATAACC GTTACTCCAT CCCAGATATG CCAGGAGACA TGGAACAGAT ATTTCCACGT 
GAGAAAGGAA ACAAGAAAAT GAAAGCAAAC ACTGTATCCG GTGGAAGAAA TAGGATCAAG 
AAAATTTTAG ACAAGACTCG TTTCAGCATT TTGCCCCCAA AACTATTTAG TGATGGCAGT 
GTAAGTCAAT CCCAGGATGA CAGCATTGTT GGAACAAGGC AGTGCAGACA CAGCCTGGCC 
ATCATGCCTG TCCCTGGAAC ACTTTCATCC AGCAGCCCGG ACCTTTTGCA GCCTACAACC 
AGCATTCTTG ATTTTTCTAA TCCTTCAGCG GTCGGTTTTT ACTATATTCC AGATCAAGTT 
ATTAGAGTTT TCAAAGCAGA TCAACAGAGT TGTTACATTA TCATCAGCAA AGACACTACA 
GCGAAAGAAG TTGTGAGTCA TGCTGTGCAT GAATTCAACT TGACGGGTGC GTCAGAGACC 
TACTCTCTCT GCGAGGTGTC TGTTAGTCCT GAGGGAGTCA TCAAACAAAG AAGGCTTCCA 
GATCAGTTCT CAAAATTAGC AGACAGGATT CAGCTCAATG GAAGGTATTA TCTGAAAAAC 
AATATGGAAA CAGAGACCTT ATGCTCAGAT GAAGATGCCC AAGAGCTGCT CAGAGAGAGT 
CAAATATCTC TTCTTCAGCT GAGCACCATT GAAGTGGCAA CTCAGCTTTC AATGAGAGAC 
TTTGAATTAT TTCGCAACAT TGAGCCTACT GAATACATTG ATGACCTTTT TAAGCTGGAC 
TCAAAAACAG GAAATGCTCA CCTGAAACAA TTTGAGGATG TCATTAACCA AGAGACATTT 
TGGGTTGCCA CAGAAATTTT ATTAGAGCCC AACCAGCTCA AAAGAATGAA AATTATTAAA 
CATTTCATTA AAATTGCCCT CCACTGTCGA GAATGCAAGA ACTTCAATTC CATGTTTGCA 
GTTATAAGTG GGCTAAATCT GGCATCGGTA GCACGACTCA GAGGTACCTG GGAAAAATTA 
CCTAGCAAGT ATGAAAAGCA TTTCAGAGAC CTTCAGGATC TTTTTGATCC ATCTAGAAAC 
ATGGCAAAAT ACCGCAATAT TCTTAGCAGT CAGAGCATGC AGCCTCCAAT TATTCCACTC 
TTTCCCGTTG TCAAGAAGGA TATTACATTT CTACATGAAG GAAATGATTC CAAAGTGGAT 
GGCCTGGTAA ATTTTGAGAA ACTCAGAATG ATTGCCAAAG AAATCCGGCA AGTTATCAGA 
ATGACCTCTG CTAACATGGA TCCGGCTGTA ATGTTCAGGC AAAGGAAGAA GAGGTGGAGG 
AGTTTGGGGT CTCTGAGCCA GGGCAGCACA AACTCAAATA TGCTCGATGT TCAAGGAGGT 
GCTCACAAAA AACGTGCCCG ACGCAGTTCG CTCCTTAATG CCAAGAAGCT ATATGAGGAT 
GCCCAGATGG CCAGAAAGGT GAAGCAGTAC CTGTCCAACC TGAATGTAGA AACTGATGAG 
GAAAAATTCC AGATCATGTC ACTTCAGTGC GAGCCTGCTT ATAGCACTTT GACAAAGAAT 
TTAAGTGAGA GAAGAGGTGG GAAATCCTCA GAAATGTCTC CAATACCCTT GAGGTCAGTT 
GGCCAAACCA CAAAAGTTCA GCAGCATCAG CAAAACAGAA TGAGCCAAGT TCTTCAAGTT 
CCTGCTGTTA ATTTGTATCC CATTAGAAAA AAGGGACCAG CAAAGGACCA CGTCACTTTT 
AATACAGGTT CTCCTCAGAA GTCATTAAGT TTGTCGGAGG AAGTAAGTAA GAAACCAGCA 
GAAGATGCTG TGTCTATGGC ATCTTCTTTG CACTCCAGTC CTCTTGCATC TCCTCAGGGC 
TCTCCACGCA AAGTTGGTAA CATCCAGAGG TCTGATAATT GCAGTCAGAT GAATCTTTCT 
GGATCCTCTT CATCGCTTGC CAGTGAAACC AGTAACAAGA ACAGTGGGCA ACGCAGCTAT 
GGAATAGGCT ATGCTCTCAT ACCTTCTGCC AAGTCAGATA ATTTCTCAGA CTCCAGCCAC 
AGCGAGATCT CCTCACGGTC CAGCATAGTG AGCAACTGCT CTGTCGACTC CATGTCTGCA 
GCCCTCCAGG ACGAGAGGGG TTTGCCCCAG TCTCTTAGTG TCGTGGATTC AGTGGGAACA 
GAAAGAAAAG AACATCCTCC ACCATTGGCT GAGTACAGTC AGCCTTGCCC AGGTTGGCCC 
CTCCCTAGAC CGGCTATAAT CAGAGGATTG GCTGTCACAT CGTCCATGAG CAATGAGGAG 
ATCTCCCATG AACATATTAC AATAGAAGCC GCAGACAGTG GCCGTGGAAG CTGGACTTCA 
TGTTCAAGTA GTTCTCACGA TAACTTCCAG AATATCCAAA TTCAGAAAAG CTGGGATCTC 
TTGAATTCTT ACCGCCACAC TCACTTGGAT GGACCTATTG CTGAAGTAGA ACCCACAAAC 
TGTTACCCTG AAGAGGCGTA TTTGTATTCC GAAGCCTTTT CCAAAAATAA TAGGCAGTCC 
AAAACCAGCG TGGAATTGGA TCAGTCTCGG CAGAGCTGGG CCTCCTCTAG CTCTCTGTCA 
GACACGTATG AAACAAACTA TGGGACGATT AAACGGAGAG GGCTGGAGAA CTCAACAATG 
GAGCAGACTG AGATTTTGGA CTCTAAACCA GGCACTGATG CAACTTACAA AACTGTTACT 
TCTAGTACAG AAAAGGGCCT AATAGTGTAC TGTGTCACCT CACCTAAGAA GGACGATAGG 
TATAGGGAGC CACCGCCCAC CCCTCCGGGA TATATGGGGA TTTCTTTAGC GGACATAAAG 
GAAGGATCGC CCCACCACCC ACACTTAAAA CCTCCAGACT ATAGTGTGGC AGTGCAGAGG 
TCAAAGATGC TACATAGTGA CCTCTCTAGA CTTTCATCAG CTTCTCTCAG TAGTGAACCA 
GTGGCCTGTA TTTCATCGAA GATGCCTCAG TGGCATACCC GGCAGCCGTC CCATCCTAAA 
CTAACAGATA TGACTGACGC AGATAGTGAA GAGGATGAAG ATGAACAAGT ATCAGCAGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_019478363.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019333908.1 Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAl76326
Clone ID Related Accession (Same CDS sequence)	XM_019478363.1
Accession Version	XM_019478363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	rap guanine nucleotide exchange factor 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017713851.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCCG CCGTGGACCC CAAGGTCCAA CAGGCCCTGC GGAAGAAGCC CCCGGAGCGG 
AGCCGCGAGG ACTTAAATAT TATCTATACT TATCTTCATG GAATGGACAT ATTGTCTCAT 
CTCAGGGAAC ATCAGCTAAG AATAATGTCT TCAAGTGCCC GCTATGAAAG ATACAAGGGA 
AACCAAGTTC TCTTTTGCTC AGAAACAATC GCCAGATGTT GGTACATTCT TCTTTCCGGA 
TCTGTGCTTA TGAAGGACTC CATGTTTTTA CCACCATGCA GTTTTGGCAA GCAGTCTGGA 
GGAAAGCGAG GATGCGAGTG TATTGTACTG GAGCCTTCTG AAATGATTGT GGTGGAGAAC 
TCCAAAGACA ACGAAGAAAA TATCTTGCAA AGAGAAGTTC CCATCAGACA GTCTCGGCGA 
AGGTTCAGGA AGATTAATCA ACGGGGAGAA CGCCAGACCA TCACTGACCA TGTGGATGCT 
AGCAGTTATC TTTCGCTTCC AGCAGATCTT ACCAAGATGC AAATTGCAGA CAATCCACAT 
CCACAGGTGA CTCATGTCCC TTCCAGCCAG TCTGGATGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGCT TGTCTGATAT ATATCAGGCT ACAGAAAGTG AGATGGGAGA TGTAGATTTA 
ACAGGCCTCC CAGAAGCACC TGTGGACTCT GAAGATGAAG AGGAGGAGGA AGATGAAGAT 
ATAGACAGAA CTTCAGATCC ATTATTGGGG CGAGACGTTG TACGAGAGTG CCTTGAAAAA 
GAACCTGCAG ATAAAACGGA TGATGACATT GAGCAATTAC TGGAATTTAT GCATCAGCTC 
CCAGCTTTTG CAAACATGAC CATGTCTGTG AGAAGAGAGC TTTGTTCAGT TATGATATTT 
GAAGTGGTAG AACAAGCAGG AGCCATCATA CTTGAAGACG GACAAGAACT TGATTCTTGG 
TATGTTATTT TAAATGGCAC TGTGGAGATC AGCTATCCAG ATGGGAAAAC TGAAAATCTC 
TGTATGGGAA ATAGTTTTGG GATTACTCCT TCTCTGGAAA AACAGTATAT GAATGGAGTG 
GTTAGAACCA AAGTAGATGA CTGTCAGTTT GTATGCATAG CCCAGCAAGA TTATTGGCGC 
ATTCTGAACC ACGTGGAAAA AAACACTCAT AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAGGAGC ACAGAGAGCT GGATCGCAGT GGAACCAGGA AAGGACACAT AGTTATTAAG 
GCAACACCTG AGCGACTCAT CACACATTTA ATAGAAGAGC ATTCCATCGT TGATCCAACA 
TACATAGAAG ACTTCCTTTT AACATATAGA ACATTTCTCT CGAGCCCCTT GGAAGTTGGG 
GATAAGCTCT TGGAGTGGTT TAAAATAGAT AGCCTCAGGG ATAAGGTTAC CAGAGTGGTC 
TTATTGTGGG TAAACAATCA TTTTAATGAT TTTGAAGGAG ATCCTGCTAT GACACGTTTT 
CTGGAAGAAT TTGAAAAAAA CTTGGAAAAT ACGAAAATGA AGGGGCACCT CAGGTTGTTG 
AATATTGCTT GTGCTGCCAA GGCAAAATGG AGGCAGATTA CCCTGCAGAA GGCTTCCAGA 
GACTCGCCTC TGTATTTCAG CCTCCTGGGA GGAAGTGAGA AGGGTTTTGG TATATTTGTA 
GAGACTGTAG AAACAGGCAG CAAAGCAGCA GAAGTTGGGC TGAAGCGTGG CGATCAGATA 
ATGGAAGTAA ATGGGCAGAA CTTTGAGAAC ATTACTTTTG TGAAAGCCCT TGAAATCTTG 
AGGAACAATA CTCATCTTTC CATTACAGTA AAGACAAACA TTTTTGTGTT TAAGGAACTG 
CTTAGCCGGA TAGGCCAGGA GAAAAACGGA GTCCCACATA TTCCAAAAAT TGCAGAAAAG 
AAAAATAACC GTTACTCCAT CCCAGATATG CCAGGAGACA TGGAACAGAT ATTTCCACGT 
GAGAAAGGAA ACAAGAAAAT GAAAGCAAAC ACTGTATCCG GTGGAAGAAA TAGGATCAAG 
AAAATTTTAG ACAAGACTCG TTTCAGCATT TTGCCCCCAA AACTATTTAG TGATGGCAGT 
GTAAGTCAAT CCCAGGATGA CAGCATTGTT GGAACAAGGC AGTGCAGACA CAGCCTGGCC 
ATCATGCCTG TCCCTGGAAC ACTTTCATCC AGCAGCCCGG ACCTTTTGCA GCCTACAACC 
AGCATTCTTG ATTTTTCTAA TCCTTCAGCG GTCGGTTTTT ACTATATTCC AGATCAAGTT 
ATTAGAGTTT TCAAAGCAGA TCAACAGAGT TGTTACATTA TCATCAGCAA AGACACTACA 
GCGAAAGAAG TTGTGAGTCA TGCTGTGCAT GAATTCAACT TGACGGGTGC GTCAGAGACC 
TACTCTCTCT GCGAGGTGTC TGTTAGTCCT GAGGGAGTCA TCAAACAAAG AAGGCTTCCA 
GATCAGTTCT CAAAATTAGC AGACAGGATT CAGCTCAATG GAAGGTATTA TCTGAAAAAC 
AATATGGAAA CAGAGACCTT ATGCTCAGAT GAAGATGCCC AAGAGCTGCT CAGAGAGAGT 
CAAATATCTC TTCTTCAGCT GAGCACCATT GAAGTGGCAA CTCAGCTTTC AATGAGAGAC 
TTTGAATTAT TTCGCAACAT TGAGCCTACT GAATACATTG ATGACCTTTT TAAGCTGGAC 
TCAAAAACAG GAAATGCTCA CCTGAAACAA TTTGAGGATG TCATTAACCA AGAGACATTT 
TGGGTTGCCA CAGAAATTTT ATTAGAGCCC AACCAGCTCA AAAGAATGAA AATTATTAAA 
CATTTCATTA AAATTGCCCT CCACTGTCGA GAATGCAAGA ACTTCAATTC CATGTTTGCA 
GTTATAAGTG GGCTAAATCT GGCATCGGTA GCACGACTCA GAGGTACCTG GGAAAAATTA 
CCTAGCAAGT ATGAAAAGCA TTTCAGAGAC CTTCAGGATC TTTTTGATCC ATCTAGAAAC 
ATGGCAAAAT ACCGCAATAT TCTTAGCAGT CAGAGCATGC AGCCTCCAAT TATTCCACTC 
TTTCCCGTTG TCAAGAAGGA TATTACATTT CTACATGAAG GAAATGATTC CAAAGTGGAT 
GGCCTGGTAA ATTTTGAGAA ACTCAGAATG ATTGCCAAAG AAATCCGGCA AGTTATCAGA 
ATGACCTCTG CTAACATGGA TCCGGCTGTA ATGTTCAGGC AAAGGAAGAA GAGGTGGAGG 
AGTTTGGGGT CTCTGAGCCA GGGCAGCACA AACTCAAATA TGCTCGATGT TCAAGGAGGT 
GCTCACAAAA AACGTGCCCG ACGCAGTTCG CTCCTTAATG CCAAGAAGCT ATATGAGGAT 
GCCCAGATGG CCAGAAAGGT GAAGCAGTAC CTGTCCAACC TGAATGTAGA AACTGATGAG 
GAAAAATTCC AGATCATGTC ACTTCAGTGC GAGCCTGCTT ATAGCACTTT GACAAAGAAT 
TTAAGTGAGA GAAGAGGTGG GAAATCCTCA GAAATGTCTC CAATACCCTT GAGGTCAGTT 
GGCCAAACCA CAAAAGTTCA GCAGCATCAG CAAAACAGAA TGAGCCAAGT TCTTCAAGTT 
CCTGCTGTTA ATTTGTATCC CATTAGAAAA AAGGGACCAG CAAAGGACCA CGTCACTTTT 
AGTTCTCCTC AGAAGTCATT AAGTTTGTCG GAGGAAGTAA GTAAGAAACC AGCAGAAGAT 
GCTGTGTCTA TGGCATCTTC TTTGCACTCC AGTCCTCTTG CATCTCCTCA GGGCTCTCCA 
CGCAAAGTTG GTAACATCCA GAGGTCTGAT AATTGCAGTC AGATGAATCT TTCTGGATCC 
TCTTCATCGC TTGCCAGTGA AACCAGTAAC AAGAACAGTG GGCAACGCAG CTATGGAATA 
GGCTATGCTC TCATACCTTC TGCCAAGTCA GATAATTTCT CAGACTCCAG CCACAGCGAG 
ATCTCCTCAC GGTCCAGCAT AGTGAGCAAC TGCTCTGTCG ACTCCATGTC TGCAGCCCTC 
CAGGACGAGA GGGGTTTGCC CCAGTCTCTT AGTGTCGTGG ATTCAGTGGG AACAGAAAGA 
AAAGAACATC CTCCACCATT GGCTGAGTAC AGTCAGCCTT GCCCAGGTTG GCCCCTCCCT 
AGACCGGCTA TAATCAGAGG ATTGGCTGTC ACATCGTCCA TGAGCAATGA GGAGATCTCC 
CATGAACATA TTACAATAGA AGCCGCAGAC AGTGGCCGTG GAAGCTGGAC TTCATGTTCA 
AGTAGTTCTC ACGATAACTT CCAGAATATC CAAATTCAGA AAAGCTGGGA TCTCTTGAAT 
TCTTACCGCC ACACTCACTT GGATGGACCT ATTGCTGAAG TAGAACCCAC AAACTGTTAC 
CCTGAAGAGG CGTATTTGTA TTCCGAAGCC TTTTCCAAAA ATAATAGGCA GTCCAAAACC 
AGCGTGGAAT TGGATCAGTC TCGGCAGAGC TGGGCCTCCT CTAGCTCTCT GTCAGACACG 
TATGAAACAA ACTATGGGAC GATTAAACGG AGAGGGCTGG AGAACTCAAC AATGGAGCAG 
ACTGAGATTT TGGACTCTAA ACCAGGCACT GATGCAACTT ACAAAACTGT TACTTCTAGT 
ACAGAAAAGG GCCTAATAGT GTACTGTGTC ACCTCACCTA AGAAGGACGA TAGGTATAGG 
GAGCCACCGC CCACCCCTCC GGGATATATG GGGATTTCTT TAGCGGACAT AAAGGAAGGA 
TCGCCCCACC ACCCACACTT AAAACCTCCA GACTATAGTG TGGCAGTGCA GAGGTCAAAG 
ATGCTACATA GTGACCTCTC TAGACTTTCA TCAGCTTCTC TCAGTAGTGA ACCAGTGGCC 
TGTATTTCAT CGAAGATGCC TCAGTGGCAT ACCCGGCAGC CGTCCCATCC TAAACTAACA 
GATATGACTG ACGCAGATAG TGAAGAGGAT GAAGATGAAC AAGTATCAGC AGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019333908.1
CDS	268..5244
Translation	MGSAVDPKVQQALRKKPPERSREDLNIIYTYLHGMDILSHLREHQLRIMSSSARYERYKGNQVLFCSETIARCWYILLSGSVLMKDSMFLPPCSFGKQSGGKRGCECIVLEPSEMIVVENSKDNEENILQREVPIRQSRRRFRKINQRGERQTITDHVDASSYLSLPADLTKMQIADNPHPQVTHVPSSQSGCSIASDSGSSSLSDIYQATESEMGDVDLTGLPEAPVDSEDEEEEEDEDIDRTSDPLLGRDVVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISYPDGKTENLCMGNSFGITPSLEKQYMNGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVKEHRELDRSGTRKGHIVIKATPERLITHLIEEHSIVDPTYIEDFLLTYRTFLSSPLEVGDKLLEWFKIDSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFEKNLENTKMKGHLRLLNIACAAKAKWRQITLQKASRDSPLYFSLLGGSEKGFGIFVETVETGSKAAEVGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLSITVKTNIFVFKELLSRIGQEKNGVPHIPKIAEKKNNRYSIPDMPGDMEQIFPREKGNKKMKANTVSGGRNRIKKILDKTRFSILPPKLFSDGSVSQSQDDSIVGTRQCRHSLAIMPVPGTLSSSSPDLLQPTTSILDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVSHAVHEFNLTGASETYSLCEVSVSPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTIEVATQLSMRDFELFRNIEPTEYIDDLFKLDSKTGNAHLKQFEDVINQETFWVATEILLEPNQLKRMKIIKHFIKIALHCRECKNFNSMFAVISGLNLASVARLRGTWEKLPSKYEKHFRDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDITFLHEGNDSKVDGLVNFEKLRMIAKEIRQVIRMTSANMDPAVMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSNLNVETDEEKFQIMSLQCEPAYSTLTKNLSERRGGKSSEMSPIPLRSVGQTTKVQQHQQNRMSQVLQVPAVNLYPIRKKGPAKDHVTFSSPQKSLSLSEEVSKKPAEDAVSMASSLHSSPLASPQGSPRKVGNIQRSDNCSQMNLSGSSSSLASETSNKNSGQRSYGIGYALIPSAKSDNFSDSSHSEISSRSSIVSNCSVDSMSAALQDERGLPQSLSVVDSVGTERKEHPPPLAEYSQPCPGWPLPRPAIIRGLAVTSSMSNEEISHEHITIEAADSGRGSWTSCSSSSHDNFQNIQIQKSWDLLNSYRHTHLDGPIAEVEPTNCYPEEAYLYSEAFSKNNRQSKTSVELDQSRQSWASSSSLSDTYETNYGTIKRRGLENSTMEQTEILDSKPGTDATYKTVTSSTEKGLIVYCVTSPKKDDRYREPPPTPPGYMGISLADIKEGSPHHPHLKPPDYSVAVQRSKMLHSDLSRLSSASLSSEPVACISSKMPQWHTRQPSHPKLTDMTDADSEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_019478363.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019478363.1

ORF Insert Sequence:

ATGGGCTCCG CCGTGGACCC CAAGGTCCAA CAGGCCCTGC GGAAGAAGCC CCCGGAGCGG 
AGCCGCGAGG ACTTAAATAT TATCTATACT TATCTTCATG GAATGGACAT ATTGTCTCAT 
CTCAGGGAAC ATCAGCTAAG AATAATGTCT TCAAGTGCCC GCTATGAAAG ATACAAGGGA 
AACCAAGTTC TCTTTTGCTC AGAAACAATC GCCAGATGTT GGTACATTCT TCTTTCCGGA 
TCTGTGCTTA TGAAGGACTC CATGTTTTTA CCACCATGCA GTTTTGGCAA GCAGTCTGGA 
GGAAAGCGAG GATGCGAGTG TATTGTACTG GAGCCTTCTG AAATGATTGT GGTGGAGAAC 
TCCAAAGACA ACGAAGAAAA TATCTTGCAA AGAGAAGTTC CCATCAGACA GTCTCGGCGA 
AGGTTCAGGA AGATTAATCA ACGGGGAGAA CGCCAGACCA TCACTGACCA TGTGGATGCT 
AGCAGTTATC TTTCGCTTCC AGCAGATCTT ACCAAGATGC AAATTGCAGA CAATCCACAT 
CCACAGGTGA CTCATGTCCC TTCCAGCCAG TCTGGATGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGCT TGTCTGATAT ATATCAGGCT ACAGAAAGTG AGATGGGAGA TGTAGATTTA 
ACAGGCCTCC CAGAAGCACC TGTGGACTCT GAAGATGAAG AGGAGGAGGA AGATGAAGAT 
ATAGACAGAA CTTCAGATCC ATTATTGGGG CGAGACGTTG TACGAGAGTG CCTTGAAAAA 
GAACCTGCAG ATAAAACGGA TGATGACATT GAGCAATTAC TGGAATTTAT GCATCAGCTC 
CCAGCTTTTG CAAACATGAC CATGTCTGTG AGAAGAGAGC TTTGTTCAGT TATGATATTT 
GAAGTGGTAG AACAAGCAGG AGCCATCATA CTTGAAGACG GACAAGAACT TGATTCTTGG 
TATGTTATTT TAAATGGCAC TGTGGAGATC AGCTATCCAG ATGGGAAAAC TGAAAATCTC 
TGTATGGGAA ATAGTTTTGG GATTACTCCT TCTCTGGAAA AACAGTATAT GAATGGAGTG 
GTTAGAACCA AAGTAGATGA CTGTCAGTTT GTATGCATAG CCCAGCAAGA TTATTGGCGC 
ATTCTGAACC ACGTGGAAAA AAACACTCAT AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAGGAGC ACAGAGAGCT GGATCGCAGT GGAACCAGGA AAGGACACAT AGTTATTAAG 
GCAACACCTG AGCGACTCAT CACACATTTA ATAGAAGAGC ATTCCATCGT TGATCCAACA 
TACATAGAAG ACTTCCTTTT AACATATAGA ACATTTCTCT CGAGCCCCTT GGAAGTTGGG 
GATAAGCTCT TGGAGTGGTT TAAAATAGAT AGCCTCAGGG ATAAGGTTAC CAGAGTGGTC 
TTATTGTGGG TAAACAATCA TTTTAATGAT TTTGAAGGAG ATCCTGCTAT GACACGTTTT 
CTGGAAGAAT TTGAAAAAAA CTTGGAAAAT ACGAAAATGA AGGGGCACCT CAGGTTGTTG 
AATATTGCTT GTGCTGCCAA GGCAAAATGG AGGCAGATTA CCCTGCAGAA GGCTTCCAGA 
GACTCGCCTC TGTATTTCAG CCTCCTGGGA GGAAGTGAGA AGGGTTTTGG TATATTTGTA 
GAGACTGTAG AAACAGGCAG CAAAGCAGCA GAAGTTGGGC TGAAGCGTGG CGATCAGATA 
ATGGAAGTAA ATGGGCAGAA CTTTGAGAAC ATTACTTTTG TGAAAGCCCT TGAAATCTTG 
AGGAACAATA CTCATCTTTC CATTACAGTA AAGACAAACA TTTTTGTGTT TAAGGAACTG 
CTTAGCCGGA TAGGCCAGGA GAAAAACGGA GTCCCACATA TTCCAAAAAT TGCAGAAAAG 
AAAAATAACC GTTACTCCAT CCCAGATATG CCAGGAGACA TGGAACAGAT ATTTCCACGT 
GAGAAAGGAA ACAAGAAAAT GAAAGCAAAC ACTGTATCCG GTGGAAGAAA TAGGATCAAG 
AAAATTTTAG ACAAGACTCG TTTCAGCATT TTGCCCCCAA AACTATTTAG TGATGGCAGT 
GTAAGTCAAT CCCAGGATGA CAGCATTGTT GGAACAAGGC AGTGCAGACA CAGCCTGGCC 
ATCATGCCTG TCCCTGGAAC ACTTTCATCC AGCAGCCCGG ACCTTTTGCA GCCTACAACC 
AGCATTCTTG ATTTTTCTAA TCCTTCAGCG GTCGGTTTTT ACTATATTCC AGATCAAGTT 
ATTAGAGTTT TCAAAGCAGA TCAACAGAGT TGTTACATTA TCATCAGCAA AGACACTACA 
GCGAAAGAAG TTGTGAGTCA TGCTGTGCAT GAATTCAACT TGACGGGTGC GTCAGAGACC 
TACTCTCTCT GCGAGGTGTC TGTTAGTCCT GAGGGAGTCA TCAAACAAAG AAGGCTTCCA 
GATCAGTTCT CAAAATTAGC AGACAGGATT CAGCTCAATG GAAGGTATTA TCTGAAAAAC 
AATATGGAAA CAGAGACCTT ATGCTCAGAT GAAGATGCCC AAGAGCTGCT CAGAGAGAGT 
CAAATATCTC TTCTTCAGCT GAGCACCATT GAAGTGGCAA CTCAGCTTTC AATGAGAGAC 
TTTGAATTAT TTCGCAACAT TGAGCCTACT GAATACATTG ATGACCTTTT TAAGCTGGAC 
TCAAAAACAG GAAATGCTCA CCTGAAACAA TTTGAGGATG TCATTAACCA AGAGACATTT 
TGGGTTGCCA CAGAAATTTT ATTAGAGCCC AACCAGCTCA AAAGAATGAA AATTATTAAA 
CATTTCATTA AAATTGCCCT CCACTGTCGA GAATGCAAGA ACTTCAATTC CATGTTTGCA 
GTTATAAGTG GGCTAAATCT GGCATCGGTA GCACGACTCA GAGGTACCTG GGAAAAATTA 
CCTAGCAAGT ATGAAAAGCA TTTCAGAGAC CTTCAGGATC TTTTTGATCC ATCTAGAAAC 
ATGGCAAAAT ACCGCAATAT TCTTAGCAGT CAGAGCATGC AGCCTCCAAT TATTCCACTC 
TTTCCCGTTG TCAAGAAGGA TATTACATTT CTACATGAAG GAAATGATTC CAAAGTGGAT 
GGCCTGGTAA ATTTTGAGAA ACTCAGAATG ATTGCCAAAG AAATCCGGCA AGTTATCAGA 
ATGACCTCTG CTAACATGGA TCCGGCTGTA ATGTTCAGGC AAAGGAAGAA GAGGTGGAGG 
AGTTTGGGGT CTCTGAGCCA GGGCAGCACA AACTCAAATA TGCTCGATGT TCAAGGAGGT 
GCTCACAAAA AACGTGCCCG ACGCAGTTCG CTCCTTAATG CCAAGAAGCT ATATGAGGAT 
GCCCAGATGG CCAGAAAGGT GAAGCAGTAC CTGTCCAACC TGAATGTAGA AACTGATGAG 
GAAAAATTCC AGATCATGTC ACTTCAGTGC GAGCCTGCTT ATAGCACTTT GACAAAGAAT 
TTAAGTGAGA GAAGAGGTGG GAAATCCTCA GAAATGTCTC CAATACCCTT GAGGTCAGTT 
GGCCAAACCA CAAAAGTTCA GCAGCATCAG CAAAACAGAA TGAGCCAAGT TCTTCAAGTT 
CCTGCTGTTA ATTTGTATCC CATTAGAAAA AAGGGACCAG CAAAGGACCA CGTCACTTTT 
AGTTCTCCTC AGAAGTCATT AAGTTTGTCG GAGGAAGTAA GTAAGAAACC AGCAGAAGAT 
GCTGTGTCTA TGGCATCTTC TTTGCACTCC AGTCCTCTTG CATCTCCTCA GGGCTCTCCA 
CGCAAAGTTG GTAACATCCA GAGGTCTGAT AATTGCAGTC AGATGAATCT TTCTGGATCC 
TCTTCATCGC TTGCCAGTGA AACCAGTAAC AAGAACAGTG GGCAACGCAG CTATGGAATA 
GGCTATGCTC TCATACCTTC TGCCAAGTCA GATAATTTCT CAGACTCCAG CCACAGCGAG 
ATCTCCTCAC GGTCCAGCAT AGTGAGCAAC TGCTCTGTCG ACTCCATGTC TGCAGCCCTC 
CAGGACGAGA GGGGTTTGCC CCAGTCTCTT AGTGTCGTGG ATTCAGTGGG AACAGAAAGA 
AAAGAACATC CTCCACCATT GGCTGAGTAC AGTCAGCCTT GCCCAGGTTG GCCCCTCCCT 
AGACCGGCTA TAATCAGAGG ATTGGCTGTC ACATCGTCCA TGAGCAATGA GGAGATCTCC 
CATGAACATA TTACAATAGA AGCCGCAGAC AGTGGCCGTG GAAGCTGGAC TTCATGTTCA 
AGTAGTTCTC ACGATAACTT CCAGAATATC CAAATTCAGA AAAGCTGGGA TCTCTTGAAT 
TCTTACCGCC ACACTCACTT GGATGGACCT ATTGCTGAAG TAGAACCCAC AAACTGTTAC 
CCTGAAGAGG CGTATTTGTA TTCCGAAGCC TTTTCCAAAA ATAATAGGCA GTCCAAAACC 
AGCGTGGAAT TGGATCAGTC TCGGCAGAGC TGGGCCTCCT CTAGCTCTCT GTCAGACACG 
TATGAAACAA ACTATGGGAC GATTAAACGG AGAGGGCTGG AGAACTCAAC AATGGAGCAG 
ACTGAGATTT TGGACTCTAA ACCAGGCACT GATGCAACTT ACAAAACTGT TACTTCTAGT 
ACAGAAAAGG GCCTAATAGT GTACTGTGTC ACCTCACCTA AGAAGGACGA TAGGTATAGG 
GAGCCACCGC CCACCCCTCC GGGATATATG GGGATTTCTT TAGCGGACAT AAAGGAAGGA 
TCGCCCCACC ACCCACACTT AAAACCTCCA GACTATAGTG TGGCAGTGCA GAGGTCAAAG 
ATGCTACATA GTGACCTCTC TAGACTTTCA TCAGCTTCTC TCAGTAGTGA ACCAGTGGCC 
TGTATTTCAT CGAAGATGCC TCAGTGGCAT ACCCGGCAGC CGTCCCATCC TAAACTAACA 
GATATGACTG ACGCAGATAG TGAAGAGGAT GAAGATGAAC AAGTATCAGC AGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_019478364.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019333909.1 Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA.

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CloneID	OAl76327
Clone ID Related Accession (Same CDS sequence)	XM_019478364.1
Accession Version	XM_019478364.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	rap guanine nucleotide exchange factor 6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017713851.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCCG CCGTGGACCC CAAGGTCCAA CAGGCCCTGC GGAAGAAGCC CCCGGAGCGG 
AGCCGCGAGG ACTTAAATAT TATCTATACT TATCTTCATG GAATGGACAT ATTGTCTCAT 
CTCAGGGAAC ATCAGCTAAG AATAATGTCT TCAAGTGCCC GCTATGAAAG ATACAAGGGA 
AACCAAGTTC TCTTTTGCTC AGAAACAATC GCCAGATGTT GGTACATTCT TCTTTCCGGA 
TCTGTGCTTA TGAAGGACTC CATGTTTTTA CCACCATGCA GTTTTGGCAA GCAGTCTGGA 
GGAAAGCGAG GATGCGAGTG TATTGTACTG GAGCCTTCTG AAATGATTGT GGTGGAGAAC 
TCCAAAGACA ACGAAGAAAA TATCTTGCAA AGAGAAGTTC CCATCAGACA GTCTCGGCGA 
AGGTTCAGGA AGATTAATCA ACGGGGAGAA CGCCAGACCA TCACTGACCA TGTGGATGCT 
AGCAGTTATC TTTCGCTTCC AGCAGATCTT ACCAAGATGC AAATTGCAGA CAATCCACAT 
CCACAGGTGA CTCATGTCCC TTCCAGCCAG TCTGGATGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGCT TGTCTGATAT ATATCAGGCT ACAGAAAGTG AGATGGGAGA TGTAGATTTA 
ACAGGCCTCC CAGAAGCACC TGTGGACTCT GAAGATGAAG AGGAGGAGGA AGATGAAGAT 
ATAGACAGAA CTTCAGATCC ATTATTGGGG CGAGACGTTG TACGAGAGTG CCTTGAAAAA 
GAACCTGCAG ATAAAACGGA TGATGACATT GAGCAATTAC TGGAATTTAT GCATCAGCTC 
CCAGCTTTTG CAAACATGAC CATGTCTGTG AGAAGAGAGC TTTGTTCAGT TATGATATTT 
GAAGTGGTAG AACAAGCAGG AGCCATCATA CTTGAAGACG GACAAGAACT TGATTCTTGG 
TATGTTATTT TAAATGGCAC TGTGGAGATC AGCTATCCAG ATGGGAAAAC TGAAAATCTC 
TGTATGGGAA ATAGTTTTGG GATTACTCCT TCTCTGGAAA AACAGTATAT GAATGGAGTG 
GTTAGAACCA AAGTAGATGA CTGTCAGTTT GTATGCATAG CCCAGCAAGA TTATTGGCGC 
ATTCTGAACC ACGTGGAAAA AAACACTCAT AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAGGAGC ACAGAGAGCT GGATCGCAGT GGAACCAGGA AAGGACACAT AGTTATTAAG 
GCAACACCTG AGCGACTCAT CACACATTTA ATAGAAGAGC ATTCCATCGT TGATCCAACA 
TACATAGAAG ACTTCCTTTT AACATATAGA ACATTTCTCT CGAGCCCCTT GGAAGTTGGG 
GATAAGCTCT TGGAGTGGTT TAAAATAGAT AGCCTCAGGG ATAAGGTTAC CAGAGTGGTC 
TTATTGTGGG TAAACAATCA TTTTAATGAT TTTGAAGGAG ATCCTGCTAT GACACGTTTT 
CTGGAAGAAT TTGAAAAAAA CTTGGAAAAT ACGAAAATGA AGGGGCACCT CAGGTTGTTG 
AATATTGCTT GTGCTGCCAA GGCAAAATGG AGGCAGATTA CCCTGCAGAA GGCTTCCAGA 
GACTCGCCTC TGTATTTCAG CCTCCTGGGA GGAAGTGAGA AGGGTTTTGG TATATTTGTA 
GAGACTGTAG AAACAGGCAG CAAAGCAGCA GAAGTTGGGC TGAAGCGTGG CGATCAGATA 
ATGGAAGTAA ATGGGCAGAA CTTTGAGAAC ATTACTTTTG TGAAAGCCCT TGAAATCTTG 
AGGAACAATA CTCATCTTTC CATTACAGTA AAGACAAACA TTTTTGTGTT TAAGGAACTG 
CTTAGCCGGA TAGGCCAGGA GAAAAACGGA GTCCCACATA TTCCAAAAAT TGCAGAAAAG 
AAAAATAACC GTTACTCCAT CCCAGATATG CCAGGAGACA TGGAACAGAT ATTTCCACGT 
GAGAAAGGAA ACAAGAAAAT GAAAGCAAAC ACTGTATCCG GTGGAAGAAA TAGGATCAAG 
AAAATTTTAG ACAAGACTCG TTTCAGCATT TTGCCCCCAA AACTATTTAG TGATGGCAGT 
GTAAGTCAAT CCCAGGATGA CAGCATTGTT GGAACAAGGC AGTGCAGACA CAGCCTGGCC 
ATCATGCCTG TCCCTGGAAC ACTTTCATCC AGCAGCCCGG ACCTTTTGCA GCCTACAACC 
AGCATTCTTG ATTTTTCTAA TCCTTCAGAT ATTCCAGATC AAGTTATTAG AGTTTTCAAA 
GCAGATCAAC AGAGTTGTTA CATTATCATC AGCAAAGACA CTACAGCGAA AGAAGTTGTG 
AGTCATGCTG TGCATGAATT CAACTTGACG GGTGCGTCAG AGACCTACTC TCTCTGCGAG 
GTGTCTGTTA GTCCTGAGGG AGTCATCAAA CAAAGAAGGC TTCCAGATCA GTTCTCAAAA 
TTAGCAGACA GGATTCAGCT CAATGGAAGG TATTATCTGA AAAACAATAT GGAAACAGAG 
ACCTTATGCT CAGATGAAGA TGCCCAAGAG CTGCTCAGAG AGAGTCAAAT ATCTCTTCTT 
CAGCTGAGCA CCATTGAAGT GGCAACTCAG CTTTCAATGA GAGACTTTGA ATTATTTCGC 
AACATTGAGC CTACTGAATA CATTGATGAC CTTTTTAAGC TGGACTCAAA AACAGGAAAT 
GCTCACCTGA AACAATTTGA GGATGTCATT AACCAAGAGA CATTTTGGGT TGCCACAGAA 
ATTTTATTAG AGCCCAACCA GCTCAAAAGA ATGAAAATTA TTAAACATTT CATTAAAATT 
GCCCTCCACT GTCGAGAATG CAAGAACTTC AATTCCATGT TTGCAGTTAT AAGTGGGCTA 
AATCTGGCAT CGGTAGCACG ACTCAGAGGT ACCTGGGAAA AATTACCTAG CAAGTATGAA 
AAGCATTTCA GAGACCTTCA GGATCTTTTT GATCCATCTA GAAACATGGC AAAATACCGC 
AATATTCTTA GCAGTCAGAG CATGCAGCCT CCAATTATTC CACTCTTTCC CGTTGTCAAG 
AAGGATATTA CATTTCTACA TGAAGGAAAT GATTCCAAAG TGGATGGCCT GGTAAATTTT 
GAGAAACTCA GAATGATTGC CAAAGAAATC CGGCAAGTTA TCAGAATGAC CTCTGCTAAC 
ATGGATCCGG CTGTAATGTT CAGGCAAAGG AAGAAGAGGT GGAGGAGTTT GGGGTCTCTG 
AGCCAGGGCA GCACAAACTC AAATATGCTC GATGTTCAAG GAGGTGCTCA CAAAAAACGT 
GCCCGACGCA GTTCGCTCCT TAATGCCAAG AAGCTATATG AGGATGCCCA GATGGCCAGA 
AAGGTGAAGC AGTACCTGTC CAACCTGAAT GTAGAAACTG ATGAGGAAAA ATTCCAGATC 
ATGTCACTTC AGTGCGAGCC TGCTTATAGC ACTTTGACAA AGAATTTAAG TGAGAGAAGA 
GGTGGGAAAT CCTCAGAAAT GTCTCCAATA CCCTTGAGGT CAGTTGGCCA AACCACAAAA 
GTTCAGCAGC ATCAGCAAAA CAGAATGAGC CAAGTTCTTC AAGTTCCTGC TGTTAATTTG 
TATCCCATTA GAAAAAAGGG ACCAGCAAAG GACCACGTCA CTTTTAATAC AGGTTCTCCT 
CAGAAGTCAT TAAGTTTGTC GGAGGAAGTA AGTAAGAAAC CAGCAGAAGA TGCTGTGTCT 
ATGGCATCTT CTTTGCACTC CAGTCCTCTT GCATCTCCTC AGGGCTCTCC ACGCAAAGTT 
GGTAACATCC AGAGGTCTGA TAATTGCAGT CAGATGAATC TTTCTGGATC CTCTTCATCG 
CTTGCCAGTG AAACCAGTAA CAAGAACAGT GGGCAACGCA GCTATGGAAT AGGCTATGCT 
CTCATACCTT CTGCCAAGTC AGATAATTTC TCAGACTCCA GCCACAGCGA GATCTCCTCA 
CGGTCCAGCA TAGTGAGCAA CTGCTCTGTC GACTCCATGT CTGCAGCCCT CCAGGACGAG 
AGGGGTTTGC CCCAGTCTCT TAGTGTCGTG GATTCAGTGG GAACAGAAAG AAAAGAACAT 
CCTCCACCAT TGGCTGAGTA CAGTCAGCCT TGCCCAGGTT GGCCCCTCCC TAGACCGGCT 
ATAATCAGAG GATTGGCTGT CACATCGTCC ATGAGCAATG AGGAGATCTC CCATGAACAT 
ATTACAATAG AAGCCGCAGA CAGTGGCCGT GGAAGCTGGA CTTCATGTTC AAGTAGTTCT 
CACGATAACT TCCAGAATAT CCAAATTCAG AAAAGCTGGG ATCTCTTGAA TTCTTACCGC 
CACACTCACT TGGATGGACC TATTGCTGAA GTAGAACCCA CAAACTGTTA CCCTGAAGAG 
GCGTATTTGT ATTCCGAAGC CTTTTCCAAA AATAATAGGC AGTCCAAAAC CAGCGTGGAA 
TTGGATCAGT CTCGGCAGAG CTGGGCCTCC TCTAGCTCTC TGTCAGACAC GTATGAAACA 
AACTATGGGA CGATTAAACG GAGAGGGCTG GAGAACTCAA CAATGGAGCA GACTGAGATT 
TTGGACTCTA AACCAGGCAC TGATGCAACT TACAAAACTG TTACTTCTAG TACAGAAAAG 
GGCCTAATAG TGTACTGTGT CACCTCACCT AAGAAGGACG ATAGGTATAG GGAGCCACCG 
CCCACCCCTC CGGGATATAT GGGGATTTCT TTAGCGGACA TAAAGGAAGG ATCGCCCCAC 
CACCCACACT TAAAACCTCC AGACTATAGT GTGGCAGTGC AGAGGTCAAA GATGCTACAT 
AGTGACCTCT CTAGACTTTC ATCAGCTTCT CTCAGTAGTG AACCAGTGGC CTGTATTTCA 
TCGAAGATGC CTCAGTGGCA TACCCGGCAG CCGTCCCATC CTAAACTAAC AGATATGACT 
GACGCAGATA GTGAAGAGGA TGAAGATGAA CAAGTATCAG CAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019333909.1
CDS	268..5235
Translation	MGSAVDPKVQQALRKKPPERSREDLNIIYTYLHGMDILSHLREHQLRIMSSSARYERYKGNQVLFCSETIARCWYILLSGSVLMKDSMFLPPCSFGKQSGGKRGCECIVLEPSEMIVVENSKDNEENILQREVPIRQSRRRFRKINQRGERQTITDHVDASSYLSLPADLTKMQIADNPHPQVTHVPSSQSGCSIASDSGSSSLSDIYQATESEMGDVDLTGLPEAPVDSEDEEEEEDEDIDRTSDPLLGRDVVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISYPDGKTENLCMGNSFGITPSLEKQYMNGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVKEHRELDRSGTRKGHIVIKATPERLITHLIEEHSIVDPTYIEDFLLTYRTFLSSPLEVGDKLLEWFKIDSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFEKNLENTKMKGHLRLLNIACAAKAKWRQITLQKASRDSPLYFSLLGGSEKGFGIFVETVETGSKAAEVGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLSITVKTNIFVFKELLSRIGQEKNGVPHIPKIAEKKNNRYSIPDMPGDMEQIFPREKGNKKMKANTVSGGRNRIKKILDKTRFSILPPKLFSDGSVSQSQDDSIVGTRQCRHSLAIMPVPGTLSSSSPDLLQPTTSILDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVSHAVHEFNLTGASETYSLCEVSVSPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTIEVATQLSMRDFELFRNIEPTEYIDDLFKLDSKTGNAHLKQFEDVINQETFWVATEILLEPNQLKRMKIIKHFIKIALHCRECKNFNSMFAVISGLNLASVARLRGTWEKLPSKYEKHFRDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDITFLHEGNDSKVDGLVNFEKLRMIAKEIRQVIRMTSANMDPAVMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSNLNVETDEEKFQIMSLQCEPAYSTLTKNLSERRGGKSSEMSPIPLRSVGQTTKVQQHQQNRMSQVLQVPAVNLYPIRKKGPAKDHVTFNTGSPQKSLSLSEEVSKKPAEDAVSMASSLHSSPLASPQGSPRKVGNIQRSDNCSQMNLSGSSSSLASETSNKNSGQRSYGIGYALIPSAKSDNFSDSSHSEISSRSSIVSNCSVDSMSAALQDERGLPQSLSVVDSVGTERKEHPPPLAEYSQPCPGWPLPRPAIIRGLAVTSSMSNEEISHEHITIEAADSGRGSWTSCSSSSHDNFQNIQIQKSWDLLNSYRHTHLDGPIAEVEPTNCYPEEAYLYSEAFSKNNRQSKTSVELDQSRQSWASSSSLSDTYETNYGTIKRRGLENSTMEQTEILDSKPGTDATYKTVTSSTEKGLIVYCVTSPKKDDRYREPPPTPPGYMGISLADIKEGSPHHPHLKPPDYSVAVQRSKMLHSDLSRLSSASLSSEPVACISSKMPQWHTRQPSHPKLTDMTDADSEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_019478364.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019478364.1

ORF Insert Sequence:

ATGGGCTCCG CCGTGGACCC CAAGGTCCAA CAGGCCCTGC GGAAGAAGCC CCCGGAGCGG 
AGCCGCGAGG ACTTAAATAT TATCTATACT TATCTTCATG GAATGGACAT ATTGTCTCAT 
CTCAGGGAAC ATCAGCTAAG AATAATGTCT TCAAGTGCCC GCTATGAAAG ATACAAGGGA 
AACCAAGTTC TCTTTTGCTC AGAAACAATC GCCAGATGTT GGTACATTCT TCTTTCCGGA 
TCTGTGCTTA TGAAGGACTC CATGTTTTTA CCACCATGCA GTTTTGGCAA GCAGTCTGGA 
GGAAAGCGAG GATGCGAGTG TATTGTACTG GAGCCTTCTG AAATGATTGT GGTGGAGAAC 
TCCAAAGACA ACGAAGAAAA TATCTTGCAA AGAGAAGTTC CCATCAGACA GTCTCGGCGA 
AGGTTCAGGA AGATTAATCA ACGGGGAGAA CGCCAGACCA TCACTGACCA TGTGGATGCT 
AGCAGTTATC TTTCGCTTCC AGCAGATCTT ACCAAGATGC AAATTGCAGA CAATCCACAT 
CCACAGGTGA CTCATGTCCC TTCCAGCCAG TCTGGATGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGCT TGTCTGATAT ATATCAGGCT ACAGAAAGTG AGATGGGAGA TGTAGATTTA 
ACAGGCCTCC CAGAAGCACC TGTGGACTCT GAAGATGAAG AGGAGGAGGA AGATGAAGAT 
ATAGACAGAA CTTCAGATCC ATTATTGGGG CGAGACGTTG TACGAGAGTG CCTTGAAAAA 
GAACCTGCAG ATAAAACGGA TGATGACATT GAGCAATTAC TGGAATTTAT GCATCAGCTC 
CCAGCTTTTG CAAACATGAC CATGTCTGTG AGAAGAGAGC TTTGTTCAGT TATGATATTT 
GAAGTGGTAG AACAAGCAGG AGCCATCATA CTTGAAGACG GACAAGAACT TGATTCTTGG 
TATGTTATTT TAAATGGCAC TGTGGAGATC AGCTATCCAG ATGGGAAAAC TGAAAATCTC 
TGTATGGGAA ATAGTTTTGG GATTACTCCT TCTCTGGAAA AACAGTATAT GAATGGAGTG 
GTTAGAACCA AAGTAGATGA CTGTCAGTTT GTATGCATAG CCCAGCAAGA TTATTGGCGC 
ATTCTGAACC ACGTGGAAAA AAACACTCAT AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAGGAGC ACAGAGAGCT GGATCGCAGT GGAACCAGGA AAGGACACAT AGTTATTAAG 
GCAACACCTG AGCGACTCAT CACACATTTA ATAGAAGAGC ATTCCATCGT TGATCCAACA 
TACATAGAAG ACTTCCTTTT AACATATAGA ACATTTCTCT CGAGCCCCTT GGAAGTTGGG 
GATAAGCTCT TGGAGTGGTT TAAAATAGAT AGCCTCAGGG ATAAGGTTAC CAGAGTGGTC 
TTATTGTGGG TAAACAATCA TTTTAATGAT TTTGAAGGAG ATCCTGCTAT GACACGTTTT 
CTGGAAGAAT TTGAAAAAAA CTTGGAAAAT ACGAAAATGA AGGGGCACCT CAGGTTGTTG 
AATATTGCTT GTGCTGCCAA GGCAAAATGG AGGCAGATTA CCCTGCAGAA GGCTTCCAGA 
GACTCGCCTC TGTATTTCAG CCTCCTGGGA GGAAGTGAGA AGGGTTTTGG TATATTTGTA 
GAGACTGTAG AAACAGGCAG CAAAGCAGCA GAAGTTGGGC TGAAGCGTGG CGATCAGATA 
ATGGAAGTAA ATGGGCAGAA CTTTGAGAAC ATTACTTTTG TGAAAGCCCT TGAAATCTTG 
AGGAACAATA CTCATCTTTC CATTACAGTA AAGACAAACA TTTTTGTGTT TAAGGAACTG 
CTTAGCCGGA TAGGCCAGGA GAAAAACGGA GTCCCACATA TTCCAAAAAT TGCAGAAAAG 
AAAAATAACC GTTACTCCAT CCCAGATATG CCAGGAGACA TGGAACAGAT ATTTCCACGT 
GAGAAAGGAA ACAAGAAAAT GAAAGCAAAC ACTGTATCCG GTGGAAGAAA TAGGATCAAG 
AAAATTTTAG ACAAGACTCG TTTCAGCATT TTGCCCCCAA AACTATTTAG TGATGGCAGT 
GTAAGTCAAT CCCAGGATGA CAGCATTGTT GGAACAAGGC AGTGCAGACA CAGCCTGGCC 
ATCATGCCTG TCCCTGGAAC ACTTTCATCC AGCAGCCCGG ACCTTTTGCA GCCTACAACC 
AGCATTCTTG ATTTTTCTAA TCCTTCAGAT ATTCCAGATC AAGTTATTAG AGTTTTCAAA 
GCAGATCAAC AGAGTTGTTA CATTATCATC AGCAAAGACA CTACAGCGAA AGAAGTTGTG 
AGTCATGCTG TGCATGAATT CAACTTGACG GGTGCGTCAG AGACCTACTC TCTCTGCGAG 
GTGTCTGTTA GTCCTGAGGG AGTCATCAAA CAAAGAAGGC TTCCAGATCA GTTCTCAAAA 
TTAGCAGACA GGATTCAGCT CAATGGAAGG TATTATCTGA AAAACAATAT GGAAACAGAG 
ACCTTATGCT CAGATGAAGA TGCCCAAGAG CTGCTCAGAG AGAGTCAAAT ATCTCTTCTT 
CAGCTGAGCA CCATTGAAGT GGCAACTCAG CTTTCAATGA GAGACTTTGA ATTATTTCGC 
AACATTGAGC CTACTGAATA CATTGATGAC CTTTTTAAGC TGGACTCAAA AACAGGAAAT 
GCTCACCTGA AACAATTTGA GGATGTCATT AACCAAGAGA CATTTTGGGT TGCCACAGAA 
ATTTTATTAG AGCCCAACCA GCTCAAAAGA ATGAAAATTA TTAAACATTT CATTAAAATT 
GCCCTCCACT GTCGAGAATG CAAGAACTTC AATTCCATGT TTGCAGTTAT AAGTGGGCTA 
AATCTGGCAT CGGTAGCACG ACTCAGAGGT ACCTGGGAAA AATTACCTAG CAAGTATGAA 
AAGCATTTCA GAGACCTTCA GGATCTTTTT GATCCATCTA GAAACATGGC AAAATACCGC 
AATATTCTTA GCAGTCAGAG CATGCAGCCT CCAATTATTC CACTCTTTCC CGTTGTCAAG 
AAGGATATTA CATTTCTACA TGAAGGAAAT GATTCCAAAG TGGATGGCCT GGTAAATTTT 
GAGAAACTCA GAATGATTGC CAAAGAAATC CGGCAAGTTA TCAGAATGAC CTCTGCTAAC 
ATGGATCCGG CTGTAATGTT CAGGCAAAGG AAGAAGAGGT GGAGGAGTTT GGGGTCTCTG 
AGCCAGGGCA GCACAAACTC AAATATGCTC GATGTTCAAG GAGGTGCTCA CAAAAAACGT 
GCCCGACGCA GTTCGCTCCT TAATGCCAAG AAGCTATATG AGGATGCCCA GATGGCCAGA 
AAGGTGAAGC AGTACCTGTC CAACCTGAAT GTAGAAACTG ATGAGGAAAA ATTCCAGATC 
ATGTCACTTC AGTGCGAGCC TGCTTATAGC ACTTTGACAA AGAATTTAAG TGAGAGAAGA 
GGTGGGAAAT CCTCAGAAAT GTCTCCAATA CCCTTGAGGT CAGTTGGCCA AACCACAAAA 
GTTCAGCAGC ATCAGCAAAA CAGAATGAGC CAAGTTCTTC AAGTTCCTGC TGTTAATTTG 
TATCCCATTA GAAAAAAGGG ACCAGCAAAG GACCACGTCA CTTTTAATAC AGGTTCTCCT 
CAGAAGTCAT TAAGTTTGTC GGAGGAAGTA AGTAAGAAAC CAGCAGAAGA TGCTGTGTCT 
ATGGCATCTT CTTTGCACTC CAGTCCTCTT GCATCTCCTC AGGGCTCTCC ACGCAAAGTT 
GGTAACATCC AGAGGTCTGA TAATTGCAGT CAGATGAATC TTTCTGGATC CTCTTCATCG 
CTTGCCAGTG AAACCAGTAA CAAGAACAGT GGGCAACGCA GCTATGGAAT AGGCTATGCT 
CTCATACCTT CTGCCAAGTC AGATAATTTC TCAGACTCCA GCCACAGCGA GATCTCCTCA 
CGGTCCAGCA TAGTGAGCAA CTGCTCTGTC GACTCCATGT CTGCAGCCCT CCAGGACGAG 
AGGGGTTTGC CCCAGTCTCT TAGTGTCGTG GATTCAGTGG GAACAGAAAG AAAAGAACAT 
CCTCCACCAT TGGCTGAGTA CAGTCAGCCT TGCCCAGGTT GGCCCCTCCC TAGACCGGCT 
ATAATCAGAG GATTGGCTGT CACATCGTCC ATGAGCAATG AGGAGATCTC CCATGAACAT 
ATTACAATAG AAGCCGCAGA CAGTGGCCGT GGAAGCTGGA CTTCATGTTC AAGTAGTTCT 
CACGATAACT TCCAGAATAT CCAAATTCAG AAAAGCTGGG ATCTCTTGAA TTCTTACCGC 
CACACTCACT TGGATGGACC TATTGCTGAA GTAGAACCCA CAAACTGTTA CCCTGAAGAG 
GCGTATTTGT ATTCCGAAGC CTTTTCCAAA AATAATAGGC AGTCCAAAAC CAGCGTGGAA 
TTGGATCAGT CTCGGCAGAG CTGGGCCTCC TCTAGCTCTC TGTCAGACAC GTATGAAACA 
AACTATGGGA CGATTAAACG GAGAGGGCTG GAGAACTCAA CAATGGAGCA GACTGAGATT 
TTGGACTCTA AACCAGGCAC TGATGCAACT TACAAAACTG TTACTTCTAG TACAGAAAAG 
GGCCTAATAG TGTACTGTGT CACCTCACCT AAGAAGGACG ATAGGTATAG GGAGCCACCG 
CCCACCCCTC CGGGATATAT GGGGATTTCT TTAGCGGACA TAAAGGAAGG ATCGCCCCAC 
CACCCACACT TAAAACCTCC AGACTATAGT GTGGCAGTGC AGAGGTCAAA GATGCTACAT 
AGTGACCTCT CTAGACTTTC ATCAGCTTCT CTCAGTAGTG AACCAGTGGC CTGTATTTCA 
TCGAAGATGC CTCAGTGGCA TACCCGGCAG CCGTCCCATC CTAAACTAAC AGATATGACT 
GACGCAGATA GTGAAGAGGA TGAAGATGAA CAAGTATCAG CAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_014597588.2 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_014453074.1 Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA.

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CloneID	OAl76328
Clone ID Related Accession (Same CDS sequence)	XM_014597588.2
Accession Version	XM_014597588.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	rap guanine nucleotide exchange factor 6 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017713851.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2016 this sequence version replaced XM_014597588.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCCG CCGTGGACCC CAAGGTCCAA CAGGCCCTGC GGAAGAAGCC CCCGGAGCGG 
AGCCGCGAGG ACTTAAATAT TATCTATACT TATCTTCATG GAATGGACAT ATTGTCTCAT 
CTCAGGGAAC ATCAGCTAAG AATAATGTCT TCAAGTGCCC GCTATGAAAG ATACAAGGGA 
AACCAAGTTC TCTTTTGCTC AGAAACAATC GCCAGATGTT GGTACATTCT TCTTTCCGGA 
TCTGTGCTTA TGAAGGACTC CATGTTTTTA CCACCATGCA GTTTTGGCAA GCAGTCTGGA 
GGAAAGCGAG GATGCGAGTG TATTGTACTG GAGCCTTCTG AAATGATTGT GGTGGAGAAC 
TCCAAAGACA ACGAAGAAAA TATCTTGCAA AGAGAAGTTC CCATCAGACA GTCTCGGCGA 
AGGTTCAGGA AGATTAATCA ACGGGGAGAA CGCCAGACCA TCACTGACCA TGTGGATGCT 
AGCAGTTATC TTTCGCTTCC AGCAGATCTT ACCAAGATGC AAATTGCAGA CAATCCACAT 
CCACAGGTGA CTCATGTCCC TTCCAGCCAG TCTGGATGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGCT TGTCTGATAT ATATCAGGCT ACAGAAAGTG AGATGGGAGA TGTAGATTTA 
ACAGGCCTCC CAGAAGCACC TGTGGACTCT GAAGATGAAG AGGAGGAGGA AGATGAAGAT 
ATAGACAGAA CTTCAGATCC ATTATTGGGG CGAGACGTTG TACGAGAGTG CCTTGAAAAA 
GAACCTGCAG ATAAAACGGA TGATGACATT GAGCAATTAC TGGAATTTAT GCATCAGCTC 
CCAGCTTTTG CAAACATGAC CATGTCTGTG AGAAGAGAGC TTTGTTCAGT TATGATATTT 
GAAGTGGTAG AACAAGCAGG AGCCATCATA CTTGAAGACG GACAAGAACT TGATTCTTGG 
TATGTTATTT TAAATGGCAC TGTGGAGATC AGCTATCCAG ATGGGAAAAC TGAAAATCTC 
TGTATGGGAA ATAGTTTTGG GATTACTCCT TCTCTGGAAA AACAGTATAT GAATGGAGTG 
GTTAGAACCA AAGTAGATGA CTGTCAGTTT GTATGCATAG CCCAGCAAGA TTATTGGCGC 
ATTCTGAACC ACGTGGAAAA AAACACTCAT AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAGGAGC ACAGAGAGCT GGATCGCAGT GGAACCAGGA AAGGACACAT AGTTATTAAG 
GCAACACCTG AGCGACTCAT CACACATTTA ATAGAAGAGC ATTCCATCGT TGATCCAACA 
TACATAGAAG ACTTCCTTTT AACATATAGA ACATTTCTCT CGAGCCCCTT GGAAGTTGGG 
GATAAGCTCT TGGAGTGGTT TAAAATAGAT AGCCTCAGGG ATAAGGTTAC CAGAGTGGTC 
TTATTGTGGG TAAACAATCA TTTTAATGAT TTTGAAGGAG ATCCTGCTAT GACACGTTTT 
CTGGAAGAAT TTGAAAAAAA CTTGGAAAAT ACGAAAATGA AGGGGCACCT CAGGTTGTTG 
AATATTGCTT GTGCTGCCAA GGCAAAATGG AGGCAGATTA CCCTGCAGAA GGCTTCCAGA 
GACTCGCCTC TGTATTTCAG CCTCCTGGGA GGAAGTGAGA AGGGTTTTGG TATATTTGTA 
GAGACTGTAG AAACAGGCAG CAAAGCAGCA GAAGTTGGGC TGAAGCGTGG CGATCAGATA 
ATGGAAGTAA ATGGGCAGAA CTTTGAGAAC ATTACTTTTG TGAAAGCCCT TGAAATCTTG 
AGGAACAATA CTCATCTTTC CATTACAGTA AAGACAAACA TTTTTGTGTT TAAGGAACTG 
CTTAGCCGGA TAGGCCAGGA GAAAAACGGA GTCCCACATA TTCCAAAAAT TGCAGAAAAG 
AAAAATAACC GTTACTCCAT CCCAGATATG CCAGGAGACA TGGAACAGAT ATTTCCACGT 
GAGAAAGGAA ACAAGAAAAT GAAAGCAAAC ACTGTATCCG GTGGAAGAAA TAGGATCAAG 
AAAATTTTAG ACAAGACTCG TTTCAGCATT TTGCCCCCAA AACTATTTAG TGATGGCAGT 
GTAAGTCAAT CCCAGGATGA CAGCATTGTT GGAACAAGGC AGTGCAGACA CAGCCTGGCC 
ATCATGCCTG TCCCTGGAAC ACTTTCATCC AGCAGCCCGG ACCTTTTGCA GCCTACAACC 
AGCATTCTTG ATTTTTCTAA TCCTTCAGCG GTCGGTTTTT ACTATATTCC AGATCAAGTT 
ATTAGAGTTT TCAAAGCAGA TCAACAGAGT TGTTACATTA TCATCAGCAA AGACACTACA 
GCGAAAGAAG TTGTGAGTCA TGCTGTGCAT GAATTCAACT TGACGGGTGC GTCAGAGACC 
TACTCTCTCT GCGAGGTGTC TGTTAGTCCT GAGGGAGTCA TCAAACAAAG AAGGCTTCCA 
GATCAGTTCT CAAAATTAGC AGACAGGATT CAGCTCAATG GAAGGTATTA TCTGAAAAAC 
AATATGGAAA CAGAGACCTT ATGCTCAGAT GAAGATGCCC AAGAGCTGCT CAGAGAGAGT 
CAAATATCTC TTCTTCAGCT GAGCACCATT GAAGTGGCAA CTCAGCTTTC AATGAGAGAC 
TTTGAATTAT TTCGCAACAT TGAGCCTACT GAATACATTG ATGACCTTTT TAAGCTGGAC 
TCAAAAACAG GAAATGCTCA CCTGAAACAA TTTGAGGATG TCATTAACCA AGAGACATTT 
TGGGTTGCCA CAGAAATTTT ATTAGAGCCC AACCAGCTCA AAAGAATGAA AATTATTAAA 
CATTTCATTA AAATTGCCCT CCACTGTCGA GAATGCAAGA ACTTCAATTC CATGTTTGCA 
GTTATAAGTG GGCTAAATCT GGCATCGGTA GCACGACTCA GAGGTACCTG GGAAAAATTA 
CCTAGCAAGT ATGAAAAGCA TTTCAGAGAC CTTCAGGATC TTTTTGATCC ATCTAGAAAC 
ATGGCAAAAT ACCGCAATAT TCTTAGCAGT CAGAGCATGC AGCCTCCAAT TATTCCACTC 
TTTCCCGTTG TCAAGAAGGA TATTACATTT CTACATGAAG GAAATGATTC CAAAGTGGAT 
GGCCTGGTAA ATTTTGAGAA ACTCAGAATG ATTGCCAAAG AAATCCGGCA AGTTATCAGA 
ATGACCTCTG CTAACATGGA TCCGGCTGTA ATGTTCAGGC AAAGGTCTCT GAGCCAGGGC 
AGCACAAACT CAAATATGCT CGATGTTCAA GGAGGTGCTC ACAAAAAACG TGCCCGACGC 
AGTTCGCTCC TTAATGCCAA GAAGCTATAT GAGGATGCCC AGATGGCCAG AAAGGTGAAG 
CAGTACCTGT CCAACCTGAA TGTAGAAACT GATGAGGAAA AATTCCAGAT CATGTCACTT 
CAGTGCGAGC CTGCTTATAG CACTTTGACA AAGAATTTAA GTGAGAGAAG AGGTGGGAAA 
TCCTCAGAAA TGTCTCCAAT ACCCTTGAGG TCAGTTGGCC AAACCACAAA AGTTCAGCAG 
CATCAGCAAA ACAGAATGAG CCAAGTTCTT CAAGTTCCTG CTGTTAATTT GTATCCCATT 
AGAAAAAAGG GACCAGCAAA GGACCACGTC ACTTTTAATA CAGGTTCTCC TCAGAAGTCA 
TTAAGTTTGT CGGAGGAAGT AAGTAAGAAA CCAGCAGAAG ATGCTGTGTC TATGGCATCT 
TCTTTGCACT CCAGTCCTCT TGCATCTCCT CAGGGCTCTC CACGCAAAGT TGGTAACATC 
CAGAGGTCTG ATAATTGCAG TCAGATGAAT CTTTCTGGAT CCTCTTCATC GCTTGCCAGT 
GAAACCAGTA ACAAGAACAG TGGGCAACGC AGCTATGGAA TAGGCTATGC TCTCATACCT 
TCTGCCAAGT CAGATAATTT CTCAGACTCC AGCCACAGCG AGATCTCCTC ACGGTCCAGC 
ATAGTGAGCA ACTGCTCTGT CGACTCCATG TCTGCAGCCC TCCAGGACGA GAGGGGTTTG 
CCCCAGTCTC TTAGTGTCGT GGATTCAGTG GGAACAGAAA GAAAAGAACA TCCTCCACCA 
TTGGCTGAGT ACAGTCAGCC TTGCCCAGGT TGGCCCCTCC CTAGACCGGC TATAATCAGA 
GGATTGGCTG TCACATCGTC CATGAGCAAT GAGGAGATCT CCCATGAACA TATTACAATA 
GAAGCCGCAG ACAGTGGCCG TGGAAGCTGG ACTTCATGTT CAAGTAGTTC TCACGATAAC 
TTCCAGAATA TCCAAATTCA GAAAAGCTGG GATCTCTTGA ATTCTTACCG CCACACTCAC 
TTGGATGGAC CTATTGCTGA AGTAGAACCC ACAAACTGTT ACCCTGAAGA GGCGTATTTG 
TATTCCGAAG CCTTTTCCAA AAATAATAGG CAGTCCAAAA CCAGCGTGGA ATTGGATCAG 
TCTCGGCAGA GCTGGGCCTC CTCTAGCTCT CTGTCAGACA CGTATGAAAC AAACTATGGG 
ACGATTAAAC GGAGAGGGCT GGAGAACTCA ACAATGGAGC AGACTGAGAT TTTGGACTCT 
AAACCAGGCA CTGATGCAAC TTACAAAACT GTTACTTCTA GTACAGAAAA GGGCCTAATA 
GTGTACTGTG TCACCTCACC TAAGAAGGAC GATAGGTATA GGGAGCCACC GCCCACCCCT 
CCGGGATATA TGGGGATTTC TTTAGCGGAC ATAAAGGAAG GATCGCCCCA CCACCCACAC 
TTAAAACCTC CAGACTATAG TGTGGCAGTG CAGAGGTCAA AGATGCTACA TAGTGACCTC 
TCTAGACTTT CATCAGCTTC TCTCAGTAGT GAACCAGTGG CCTGTATTTC ATCGAAGATG 
CCTCAGTGGC ATACCCGGCA GCCGTCCCAT CCTAAACTAA CAGATATGAC TGACGCAGAT 
AGTGAAGAGG ATGAAGATGA ACAAGTATCA GCAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014453074.1
CDS	268..5226
Translation	MGSAVDPKVQQALRKKPPERSREDLNIIYTYLHGMDILSHLREHQLRIMSSSARYERYKGNQVLFCSETIARCWYILLSGSVLMKDSMFLPPCSFGKQSGGKRGCECIVLEPSEMIVVENSKDNEENILQREVPIRQSRRRFRKINQRGERQTITDHVDASSYLSLPADLTKMQIADNPHPQVTHVPSSQSGCSIASDSGSSSLSDIYQATESEMGDVDLTGLPEAPVDSEDEEEEEDEDIDRTSDPLLGRDVVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISYPDGKTENLCMGNSFGITPSLEKQYMNGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVKEHRELDRSGTRKGHIVIKATPERLITHLIEEHSIVDPTYIEDFLLTYRTFLSSPLEVGDKLLEWFKIDSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFEKNLENTKMKGHLRLLNIACAAKAKWRQITLQKASRDSPLYFSLLGGSEKGFGIFVETVETGSKAAEVGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLSITVKTNIFVFKELLSRIGQEKNGVPHIPKIAEKKNNRYSIPDMPGDMEQIFPREKGNKKMKANTVSGGRNRIKKILDKTRFSILPPKLFSDGSVSQSQDDSIVGTRQCRHSLAIMPVPGTLSSSSPDLLQPTTSILDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVSHAVHEFNLTGASETYSLCEVSVSPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTIEVATQLSMRDFELFRNIEPTEYIDDLFKLDSKTGNAHLKQFEDVINQETFWVATEILLEPNQLKRMKIIKHFIKIALHCRECKNFNSMFAVISGLNLASVARLRGTWEKLPSKYEKHFRDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDITFLHEGNDSKVDGLVNFEKLRMIAKEIRQVIRMTSANMDPAVMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSNLNVETDEEKFQIMSLQCEPAYSTLTKNLSERRGGKSSEMSPIPLRSVGQTTKVQQHQQNRMSQVLQVPAVNLYPIRKKGPAKDHVTFNTGSPQKSLSLSEEVSKKPAEDAVSMASSLHSSPLASPQGSPRKVGNIQRSDNCSQMNLSGSSSSLASETSNKNSGQRSYGIGYALIPSAKSDNFSDSSHSEISSRSSIVSNCSVDSMSAALQDERGLPQSLSVVDSVGTERKEHPPPLAEYSQPCPGWPLPRPAIIRGLAVTSSMSNEEISHEHITIEAADSGRGSWTSCSSSSHDNFQNIQIQKSWDLLNSYRHTHLDGPIAEVEPTNCYPEEAYLYSEAFSKNNRQSKTSVELDQSRQSWASSSSLSDTYETNYGTIKRRGLENSTMEQTEILDSKPGTDATYKTVTSSTEKGLIVYCVTSPKKDDRYREPPPTPPGYMGISLADIKEGSPHHPHLKPPDYSVAVQRSKMLHSDLSRLSSASLSSEPVACISSKMPQWHTRQPSHPKLTDMTDADSEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014597588.2
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014597588.2

ORF Insert Sequence:

ATGGGCTCCG CCGTGGACCC CAAGGTCCAA CAGGCCCTGC GGAAGAAGCC CCCGGAGCGG 
AGCCGCGAGG ACTTAAATAT TATCTATACT TATCTTCATG GAATGGACAT ATTGTCTCAT 
CTCAGGGAAC ATCAGCTAAG AATAATGTCT TCAAGTGCCC GCTATGAAAG ATACAAGGGA 
AACCAAGTTC TCTTTTGCTC AGAAACAATC GCCAGATGTT GGTACATTCT TCTTTCCGGA 
TCTGTGCTTA TGAAGGACTC CATGTTTTTA CCACCATGCA GTTTTGGCAA GCAGTCTGGA 
GGAAAGCGAG GATGCGAGTG TATTGTACTG GAGCCTTCTG AAATGATTGT GGTGGAGAAC 
TCCAAAGACA ACGAAGAAAA TATCTTGCAA AGAGAAGTTC CCATCAGACA GTCTCGGCGA 
AGGTTCAGGA AGATTAATCA ACGGGGAGAA CGCCAGACCA TCACTGACCA TGTGGATGCT 
AGCAGTTATC TTTCGCTTCC AGCAGATCTT ACCAAGATGC AAATTGCAGA CAATCCACAT 
CCACAGGTGA CTCATGTCCC TTCCAGCCAG TCTGGATGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGCT TGTCTGATAT ATATCAGGCT ACAGAAAGTG AGATGGGAGA TGTAGATTTA 
ACAGGCCTCC CAGAAGCACC TGTGGACTCT GAAGATGAAG AGGAGGAGGA AGATGAAGAT 
ATAGACAGAA CTTCAGATCC ATTATTGGGG CGAGACGTTG TACGAGAGTG CCTTGAAAAA 
GAACCTGCAG ATAAAACGGA TGATGACATT GAGCAATTAC TGGAATTTAT GCATCAGCTC 
CCAGCTTTTG CAAACATGAC CATGTCTGTG AGAAGAGAGC TTTGTTCAGT TATGATATTT 
GAAGTGGTAG AACAAGCAGG AGCCATCATA CTTGAAGACG GACAAGAACT TGATTCTTGG 
TATGTTATTT TAAATGGCAC TGTGGAGATC AGCTATCCAG ATGGGAAAAC TGAAAATCTC 
TGTATGGGAA ATAGTTTTGG GATTACTCCT TCTCTGGAAA AACAGTATAT GAATGGAGTG 
GTTAGAACCA AAGTAGATGA CTGTCAGTTT GTATGCATAG CCCAGCAAGA TTATTGGCGC 
ATTCTGAACC ACGTGGAAAA AAACACTCAT AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAGGAGC ACAGAGAGCT GGATCGCAGT GGAACCAGGA AAGGACACAT AGTTATTAAG 
GCAACACCTG AGCGACTCAT CACACATTTA ATAGAAGAGC ATTCCATCGT TGATCCAACA 
TACATAGAAG ACTTCCTTTT AACATATAGA ACATTTCTCT CGAGCCCCTT GGAAGTTGGG 
GATAAGCTCT TGGAGTGGTT TAAAATAGAT AGCCTCAGGG ATAAGGTTAC CAGAGTGGTC 
TTATTGTGGG TAAACAATCA TTTTAATGAT TTTGAAGGAG ATCCTGCTAT GACACGTTTT 
CTGGAAGAAT TTGAAAAAAA CTTGGAAAAT ACGAAAATGA AGGGGCACCT CAGGTTGTTG 
AATATTGCTT GTGCTGCCAA GGCAAAATGG AGGCAGATTA CCCTGCAGAA GGCTTCCAGA 
GACTCGCCTC TGTATTTCAG CCTCCTGGGA GGAAGTGAGA AGGGTTTTGG TATATTTGTA 
GAGACTGTAG AAACAGGCAG CAAAGCAGCA GAAGTTGGGC TGAAGCGTGG CGATCAGATA 
ATGGAAGTAA ATGGGCAGAA CTTTGAGAAC ATTACTTTTG TGAAAGCCCT TGAAATCTTG 
AGGAACAATA CTCATCTTTC CATTACAGTA AAGACAAACA TTTTTGTGTT TAAGGAACTG 
CTTAGCCGGA TAGGCCAGGA GAAAAACGGA GTCCCACATA TTCCAAAAAT TGCAGAAAAG 
AAAAATAACC GTTACTCCAT CCCAGATATG CCAGGAGACA TGGAACAGAT ATTTCCACGT 
GAGAAAGGAA ACAAGAAAAT GAAAGCAAAC ACTGTATCCG GTGGAAGAAA TAGGATCAAG 
AAAATTTTAG ACAAGACTCG TTTCAGCATT TTGCCCCCAA AACTATTTAG TGATGGCAGT 
GTAAGTCAAT CCCAGGATGA CAGCATTGTT GGAACAAGGC AGTGCAGACA CAGCCTGGCC 
ATCATGCCTG TCCCTGGAAC ACTTTCATCC AGCAGCCCGG ACCTTTTGCA GCCTACAACC 
AGCATTCTTG ATTTTTCTAA TCCTTCAGCG GTCGGTTTTT ACTATATTCC AGATCAAGTT 
ATTAGAGTTT TCAAAGCAGA TCAACAGAGT TGTTACATTA TCATCAGCAA AGACACTACA 
GCGAAAGAAG TTGTGAGTCA TGCTGTGCAT GAATTCAACT TGACGGGTGC GTCAGAGACC 
TACTCTCTCT GCGAGGTGTC TGTTAGTCCT GAGGGAGTCA TCAAACAAAG AAGGCTTCCA 
GATCAGTTCT CAAAATTAGC AGACAGGATT CAGCTCAATG GAAGGTATTA TCTGAAAAAC 
AATATGGAAA CAGAGACCTT ATGCTCAGAT GAAGATGCCC AAGAGCTGCT CAGAGAGAGT 
CAAATATCTC TTCTTCAGCT GAGCACCATT GAAGTGGCAA CTCAGCTTTC AATGAGAGAC 
TTTGAATTAT TTCGCAACAT TGAGCCTACT GAATACATTG ATGACCTTTT TAAGCTGGAC 
TCAAAAACAG GAAATGCTCA CCTGAAACAA TTTGAGGATG TCATTAACCA AGAGACATTT 
TGGGTTGCCA CAGAAATTTT ATTAGAGCCC AACCAGCTCA AAAGAATGAA AATTATTAAA 
CATTTCATTA AAATTGCCCT CCACTGTCGA GAATGCAAGA ACTTCAATTC CATGTTTGCA 
GTTATAAGTG GGCTAAATCT GGCATCGGTA GCACGACTCA GAGGTACCTG GGAAAAATTA 
CCTAGCAAGT ATGAAAAGCA TTTCAGAGAC CTTCAGGATC TTTTTGATCC ATCTAGAAAC 
ATGGCAAAAT ACCGCAATAT TCTTAGCAGT CAGAGCATGC AGCCTCCAAT TATTCCACTC 
TTTCCCGTTG TCAAGAAGGA TATTACATTT CTACATGAAG GAAATGATTC CAAAGTGGAT 
GGCCTGGTAA ATTTTGAGAA ACTCAGAATG ATTGCCAAAG AAATCCGGCA AGTTATCAGA 
ATGACCTCTG CTAACATGGA TCCGGCTGTA ATGTTCAGGC AAAGGTCTCT GAGCCAGGGC 
AGCACAAACT CAAATATGCT CGATGTTCAA GGAGGTGCTC ACAAAAAACG TGCCCGACGC 
AGTTCGCTCC TTAATGCCAA GAAGCTATAT GAGGATGCCC AGATGGCCAG AAAGGTGAAG 
CAGTACCTGT CCAACCTGAA TGTAGAAACT GATGAGGAAA AATTCCAGAT CATGTCACTT 
CAGTGCGAGC CTGCTTATAG CACTTTGACA AAGAATTTAA GTGAGAGAAG AGGTGGGAAA 
TCCTCAGAAA TGTCTCCAAT ACCCTTGAGG TCAGTTGGCC AAACCACAAA AGTTCAGCAG 
CATCAGCAAA ACAGAATGAG CCAAGTTCTT CAAGTTCCTG CTGTTAATTT GTATCCCATT 
AGAAAAAAGG GACCAGCAAA GGACCACGTC ACTTTTAATA CAGGTTCTCC TCAGAAGTCA 
TTAAGTTTGT CGGAGGAAGT AAGTAAGAAA CCAGCAGAAG ATGCTGTGTC TATGGCATCT 
TCTTTGCACT CCAGTCCTCT TGCATCTCCT CAGGGCTCTC CACGCAAAGT TGGTAACATC 
CAGAGGTCTG ATAATTGCAG TCAGATGAAT CTTTCTGGAT CCTCTTCATC GCTTGCCAGT 
GAAACCAGTA ACAAGAACAG TGGGCAACGC AGCTATGGAA TAGGCTATGC TCTCATACCT 
TCTGCCAAGT CAGATAATTT CTCAGACTCC AGCCACAGCG AGATCTCCTC ACGGTCCAGC 
ATAGTGAGCA ACTGCTCTGT CGACTCCATG TCTGCAGCCC TCCAGGACGA GAGGGGTTTG 
CCCCAGTCTC TTAGTGTCGT GGATTCAGTG GGAACAGAAA GAAAAGAACA TCCTCCACCA 
TTGGCTGAGT ACAGTCAGCC TTGCCCAGGT TGGCCCCTCC CTAGACCGGC TATAATCAGA 
GGATTGGCTG TCACATCGTC CATGAGCAAT GAGGAGATCT CCCATGAACA TATTACAATA 
GAAGCCGCAG ACAGTGGCCG TGGAAGCTGG ACTTCATGTT CAAGTAGTTC TCACGATAAC 
TTCCAGAATA TCCAAATTCA GAAAAGCTGG GATCTCTTGA ATTCTTACCG CCACACTCAC 
TTGGATGGAC CTATTGCTGA AGTAGAACCC ACAAACTGTT ACCCTGAAGA GGCGTATTTG 
TATTCCGAAG CCTTTTCCAA AAATAATAGG CAGTCCAAAA CCAGCGTGGA ATTGGATCAG 
TCTCGGCAGA GCTGGGCCTC CTCTAGCTCT CTGTCAGACA CGTATGAAAC AAACTATGGG 
ACGATTAAAC GGAGAGGGCT GGAGAACTCA ACAATGGAGC AGACTGAGAT TTTGGACTCT 
AAACCAGGCA CTGATGCAAC TTACAAAACT GTTACTTCTA GTACAGAAAA GGGCCTAATA 
GTGTACTGTG TCACCTCACC TAAGAAGGAC GATAGGTATA GGGAGCCACC GCCCACCCCT 
CCGGGATATA TGGGGATTTC TTTAGCGGAC ATAAAGGAAG GATCGCCCCA CCACCCACAC 
TTAAAACCTC CAGACTATAG TGTGGCAGTG CAGAGGTCAA AGATGCTACA TAGTGACCTC 
TCTAGACTTT CATCAGCTTC TCTCAGTAGT GAACCAGTGG CCTGTATTTC ATCGAAGATG 
CCTCAGTGGC ATACCCGGCA GCCGTCCCAT CCTAAACTAA CAGATATGAC TGACGCAGAT 
AGTGAAGAGG ATGAAGATGA ACAAGTATCA GCAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_019478366.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019333911.1 Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA.

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CloneID	OAl76329
Clone ID Related Accession (Same CDS sequence)	XM_019478366.1
Accession Version	XM_019478366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	rap guanine nucleotide exchange factor 6 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017713851.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCCG CCGTGGACCC CAAGGTCCAA CAGGCCCTGC GGAAGAAGCC CCCGGAGCGG 
AGCCGCGAGG ACTTAAATAT TATCTATACT TATCTTCATG GAATGGACAT ATTGTCTCAT 
CTCAGGGAAC ATCAGCTAAG AATAATGTCT TCAAGTGCCC GCTATGAAAG ATACAAGGGA 
AACCAAGTTC TCTTTTGCTC AGAAACAATC GCCAGATGTT GGTACATTCT TCTTTCCGGA 
TCTGTGCTTA TGAAGGACTC CATGTTTTTA CCACCATGCA GTTTTGGCAA GCAGTCTGGA 
GGAAAGCGAG GATGCGAGTG TATTGTACTG GAGCCTTCTG AAATGATTGT GGTGGAGAAC 
TCCAAAGACA ACGAAGAAAA TATCTTGCAA AGAGAAGTTC CCATCAGACA GTCTCGGCGA 
AGGTTCAGGA AGATTAATCA ACGGGGAGAA CGCCAGACCA TCACTGACCA TGTGGATGCT 
AGCAGTTATC TTTCGCTTCC AGCAGATCTT ACCAAGATGC AAATTGCAGA CAATCCACAT 
CCACAGGTGA CTCATGTCCC TTCCAGCCAG TCTGGATGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGCT TGTCTGATAT ATATCAGGCT ACAGAAAGTG AGATGGGAGA TGTAGATTTA 
ACAGGCCTCC CAGAAGCACC TGTGGACTCT GAAGATGAAG AGGAGGAGGA AGATGAAGAT 
ATAGACAGAA CTTCAGATCC ATTATTGGGG CGAGACGTTG TACGAGAGTG CCTTGAAAAA 
GAACCTGCAG ATAAAACGGA TGATGACATT GAGCAATTAC TGGAATTTAT GCATCAGCTC 
CCAGCTTTTG CAAACATGAC CATGTCTGTG AGAAGAGAGC TTTGTTCAGT TATGATATTT 
GAAGTGGTAG AACAAGCAGG AGCCATCATA CTTGAAGACG GACAAGAACT TGATTCTTGG 
TATGTTATTT TAAATGGCAC TGTGGAGATC AGCTATCCAG ATGGGAAAAC TGAAAATCTC 
TGTATGGGAA ATAGTTTTGG GATTACTCCT TCTCTGGAAA AACAGTATAT GAATGGAGTG 
GTTAGAACCA AAGTAGATGA CTGTCAGTTT GTATGCATAG CCCAGCAAGA TTATTGGCGC 
ATTCTGAACC ACGTGGAAAA AAACACTCAT AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAGGAGC ACAGAGAGCT GGATCGCAGT GGAACCAGGA AAGGACACAT AGTTATTAAG 
GCAACACCTG AGCGACTCAT CACACATTTA ATAGAAGAGC ATTCCATCGT TGATCCAACA 
TACATAGAAG ACTTCCTTTT AACATATAGA ACATTTCTCT CGAGCCCCTT GGAAGTTGGG 
GATAAGCTCT TGGAGTGGTT TAAAATAGAT AGCCTCAGGG ATAAGGTTAC CAGAGTGGTC 
TTATTGTGGG TAAACAATCA TTTTAATGAT TTTGAAGGAG ATCCTGCTAT GACACGTTTT 
CTGGAAGAAT TTGAAAAAAA CTTGGAAAAT ACGAAAATGA AGGGGCACCT CAGGTTGTTG 
AATATTGCTT GTGCTGCCAA GGCAAAATGG AGGCAGATTA CCCTGCAGAA GGCTTCCAGA 
GACTCGCCTC TGTATTTCAG CCTCCTGGGA GGAAGTGAGA AGGGTTTTGG TATATTTGTA 
GAGACTGTAG AAACAGGCAG CAAAGCAGCA GAAGTTGGGC TGAAGCGTGG CGATCAGATA 
ATGGAAGTAA ATGGGCAGAA CTTTGAGAAC ATTACTTTTG TGAAAGCCCT TGAAATCTTG 
AGGAACAATA CTCATCTTTC CATTACAGTA AAGACAAACA TTTTTGTGTT TAAGGAACTG 
CTTAGCCGGA TAGGCCAGGA GAAAAACGGA GTCCCACATA TTCCAAAAAT TGCAGAAAAG 
AAAAATAACC GTTACTCCAT CCCAGATATG CCAGGAGACA TGGAACAGAT ATTTCCACGT 
GAGAAAGGAA ACAAGAAAAT GAAAGCAAAC ACTGTATCCG GTGGAAGAAA TAGGATCAAG 
AAAATTTTAG ACAAGACTCG TTTCAGCATT TTGCCCCCAA AACTATTTAG TGATGGCAGT 
GTAAGTCAAT CCCAGGATGA CAGCATTGTT GGAACAAGGC AGTGCAGACA CAGCCTGGCC 
ATCATGCCTG TCCCTGGAAC ACTTTCATCC AGCAGCCCGG ACCTTTTGCA GCCTACAACC 
AGCATTCTTG ATTTTTCTAA TCCTTCAGAT ATTCCAGATC AAGTTATTAG AGTTTTCAAA 
GCAGATCAAC AGAGTTGTTA CATTATCATC AGCAAAGACA CTACAGCGAA AGAAGTTGTG 
AGTCATGCTG TGCATGAATT CAACTTGACG GGTGCGTCAG AGACCTACTC TCTCTGCGAG 
GTGTCTGTTA GTCCTGAGGG AGTCATCAAA CAAAGAAGGC TTCCAGATCA GTTCTCAAAA 
TTAGCAGACA GGATTCAGCT CAATGGAAGG TATTATCTGA AAAACAATAT GGAAACAGAG 
ACCTTATGCT CAGATGAAGA TGCCCAAGAG CTGCTCAGAG AGAGTCAAAT ATCTCTTCTT 
CAGCTGAGCA CCATTGAAGT GGCAACTCAG CTTTCAATGA GAGACTTTGA ATTATTTCGC 
AACATTGAGC CTACTGAATA CATTGATGAC CTTTTTAAGC TGGACTCAAA AACAGGAAAT 
GCTCACCTGA AACAATTTGA GGATGTCATT AACCAAGAGA CATTTTGGGT TGCCACAGAA 
ATTTTATTAG AGCCCAACCA GCTCAAAAGA ATGAAAATTA TTAAACATTT CATTAAAATT 
GCCCTCCACT GTCGAGAATG CAAGAACTTC AATTCCATGT TTGCAGTTAT AAGTGGGCTA 
AATCTGGCAT CGGTAGCACG ACTCAGAGGT ACCTGGGAAA AATTACCTAG CAAGTATGAA 
AAGCATTTCA GAGACCTTCA GGATCTTTTT GATCCATCTA GAAACATGGC AAAATACCGC 
AATATTCTTA GCAGTCAGAG CATGCAGCCT CCAATTATTC CACTCTTTCC CGTTGTCAAG 
AAGGATATTA CATTTCTACA TGAAGGAAAT GATTCCAAAG TGGATGGCCT GGTAAATTTT 
GAGAAACTCA GAATGATTGC CAAAGAAATC CGGCAAGTTA TCAGAATGAC CTCTGCTAAC 
ATGGATCCGG CTGTAATGTT CAGGCAAAGG TCTCTGAGCC AGGGCAGCAC AAACTCAAAT 
ATGCTCGATG TTCAAGGAGG TGCTCACAAA AAACGTGCCC GACGCAGTTC GCTCCTTAAT 
GCCAAGAAGC TATATGAGGA TGCCCAGATG GCCAGAAAGG TGAAGCAGTA CCTGTCCAAC 
CTGAATGTAG AAACTGATGA GGAAAAATTC CAGATCATGT CACTTCAGTG CGAGCCTGCT 
TATAGCACTT TGACAAAGAA TTTAAGTGAG AGAAGAGGTG GGAAATCCTC AGAAATGTCT 
CCAATACCCT TGAGGTCAGT TGGCCAAACC ACAAAAGTTC AGCAGCATCA GCAAAACAGA 
ATGAGCCAAG TTCTTCAAGT TCCTGCTGTT AATTTGTATC CCATTAGAAA AAAGGGACCA 
GCAAAGGACC ACGTCACTTT TAATACAGGT TCTCCTCAGA AGTCATTAAG TTTGTCGGAG 
GAAGTAAGTA AGAAACCAGC AGAAGATGCT GTGTCTATGG CATCTTCTTT GCACTCCAGT 
CCTCTTGCAT CTCCTCAGGG CTCTCCACGC AAAGTTGGTA ACATCCAGAG GTCTGATAAT 
TGCAGTCAGA TGAATCTTTC TGGATCCTCT TCATCGCTTG CCAGTGAAAC CAGTAACAAG 
AACAGTGGGC AACGCAGCTA TGGAATAGGC TATGCTCTCA TACCTTCTGC CAAGTCAGAT 
AATTTCTCAG ACTCCAGCCA CAGCGAGATC TCCTCACGGT CCAGCATAGT GAGCAACTGC 
TCTGTCGACT CCATGTCTGC AGCCCTCCAG GACGAGAGGG GTTTGCCCCA GTCTCTTAGT 
GTCGTGGATT CAGTGGGAAC AGAAAGAAAA GAACATCCTC CACCATTGGC TGAGTACAGT 
CAGCCTTGCC CAGGTTGGCC CCTCCCTAGA CCGGCTATAA TCAGAGGATT GGCTGTCACA 
TCGTCCATGA GCAATGAGGA GATCTCCCAT GAACATATTA CAATAGAAGC CGCAGACAGT 
GGCCGTGGAA GCTGGACTTC ATGTTCAAGT AGTTCTCACG ATAACTTCCA GAATATCCAA 
ATTCAGAAAA GCTGGGATCT CTTGAATTCT TACCGCCACA CTCACTTGGA TGGACCTATT 
GCTGAAGTAG AACCCACAAA CTGTTACCCT GAAGAGGCGT ATTTGTATTC CGAAGCCTTT 
TCCAAAAATA ATAGGCAGTC CAAAACCAGC GTGGAATTGG ATCAGTCTCG GCAGAGCTGG 
GCCTCCTCTA GCTCTCTGTC AGACACGTAT GAAACAAACT ATGGGACGAT TAAACGGAGA 
GGGCTGGAGA ACTCAACAAT GGAGCAGACT GAGATTTTGG ACTCTAAACC AGGCACTGAT 
GCAACTTACA AAACTGTTAC TTCTAGTACA GAAAAGGGCC TAATAGTGTA CTGTGTCACC 
TCACCTAAGA AGGACGATAG GTATAGGGAG CCACCGCCCA CCCCTCCGGG ATATATGGGG 
ATTTCTTTAG CGGACATAAA GGAAGGATCG CCCCACCACC CACACTTAAA ACCTCCAGAC 
TATAGTGTGG CAGTGCAGAG GTCAAAGATG CTACATAGTG ACCTCTCTAG ACTTTCATCA 
GCTTCTCTCA GTAGTGAACC AGTGGCCTGT ATTTCATCGA AGATGCCTCA GTGGCATACC 
CGGCAGCCGT CCCATCCTAA ACTAACAGAT ATGACTGACG CAGATAGTGA AGAGGATGAA 
GATGAACAAG TATCAGCAGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019333911.1
CDS	268..5211
Translation	MGSAVDPKVQQALRKKPPERSREDLNIIYTYLHGMDILSHLREHQLRIMSSSARYERYKGNQVLFCSETIARCWYILLSGSVLMKDSMFLPPCSFGKQSGGKRGCECIVLEPSEMIVVENSKDNEENILQREVPIRQSRRRFRKINQRGERQTITDHVDASSYLSLPADLTKMQIADNPHPQVTHVPSSQSGCSIASDSGSSSLSDIYQATESEMGDVDLTGLPEAPVDSEDEEEEEDEDIDRTSDPLLGRDVVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISYPDGKTENLCMGNSFGITPSLEKQYMNGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVKEHRELDRSGTRKGHIVIKATPERLITHLIEEHSIVDPTYIEDFLLTYRTFLSSPLEVGDKLLEWFKIDSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFEKNLENTKMKGHLRLLNIACAAKAKWRQITLQKASRDSPLYFSLLGGSEKGFGIFVETVETGSKAAEVGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLSITVKTNIFVFKELLSRIGQEKNGVPHIPKIAEKKNNRYSIPDMPGDMEQIFPREKGNKKMKANTVSGGRNRIKKILDKTRFSILPPKLFSDGSVSQSQDDSIVGTRQCRHSLAIMPVPGTLSSSSPDLLQPTTSILDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVSHAVHEFNLTGASETYSLCEVSVSPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTIEVATQLSMRDFELFRNIEPTEYIDDLFKLDSKTGNAHLKQFEDVINQETFWVATEILLEPNQLKRMKIIKHFIKIALHCRECKNFNSMFAVISGLNLASVARLRGTWEKLPSKYEKHFRDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDITFLHEGNDSKVDGLVNFEKLRMIAKEIRQVIRMTSANMDPAVMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSNLNVETDEEKFQIMSLQCEPAYSTLTKNLSERRGGKSSEMSPIPLRSVGQTTKVQQHQQNRMSQVLQVPAVNLYPIRKKGPAKDHVTFNTGSPQKSLSLSEEVSKKPAEDAVSMASSLHSSPLASPQGSPRKVGNIQRSDNCSQMNLSGSSSSLASETSNKNSGQRSYGIGYALIPSAKSDNFSDSSHSEISSRSSIVSNCSVDSMSAALQDERGLPQSLSVVDSVGTERKEHPPPLAEYSQPCPGWPLPRPAIIRGLAVTSSMSNEEISHEHITIEAADSGRGSWTSCSSSSHDNFQNIQIQKSWDLLNSYRHTHLDGPIAEVEPTNCYPEEAYLYSEAFSKNNRQSKTSVELDQSRQSWASSSSLSDTYETNYGTIKRRGLENSTMEQTEILDSKPGTDATYKTVTSSTEKGLIVYCVTSPKKDDRYREPPPTPPGYMGISLADIKEGSPHHPHLKPPDYSVAVQRSKMLHSDLSRLSSASLSSEPVACISSKMPQWHTRQPSHPKLTDMTDADSEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_019478366.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019478366.1

ORF Insert Sequence:

ATGGGCTCCG CCGTGGACCC CAAGGTCCAA CAGGCCCTGC GGAAGAAGCC CCCGGAGCGG 
AGCCGCGAGG ACTTAAATAT TATCTATACT TATCTTCATG GAATGGACAT ATTGTCTCAT 
CTCAGGGAAC ATCAGCTAAG AATAATGTCT TCAAGTGCCC GCTATGAAAG ATACAAGGGA 
AACCAAGTTC TCTTTTGCTC AGAAACAATC GCCAGATGTT GGTACATTCT TCTTTCCGGA 
TCTGTGCTTA TGAAGGACTC CATGTTTTTA CCACCATGCA GTTTTGGCAA GCAGTCTGGA 
GGAAAGCGAG GATGCGAGTG TATTGTACTG GAGCCTTCTG AAATGATTGT GGTGGAGAAC 
TCCAAAGACA ACGAAGAAAA TATCTTGCAA AGAGAAGTTC CCATCAGACA GTCTCGGCGA 
AGGTTCAGGA AGATTAATCA ACGGGGAGAA CGCCAGACCA TCACTGACCA TGTGGATGCT 
AGCAGTTATC TTTCGCTTCC AGCAGATCTT ACCAAGATGC AAATTGCAGA CAATCCACAT 
CCACAGGTGA CTCATGTCCC TTCCAGCCAG TCTGGATGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGCT TGTCTGATAT ATATCAGGCT ACAGAAAGTG AGATGGGAGA TGTAGATTTA 
ACAGGCCTCC CAGAAGCACC TGTGGACTCT GAAGATGAAG AGGAGGAGGA AGATGAAGAT 
ATAGACAGAA CTTCAGATCC ATTATTGGGG CGAGACGTTG TACGAGAGTG CCTTGAAAAA 
GAACCTGCAG ATAAAACGGA TGATGACATT GAGCAATTAC TGGAATTTAT GCATCAGCTC 
CCAGCTTTTG CAAACATGAC CATGTCTGTG AGAAGAGAGC TTTGTTCAGT TATGATATTT 
GAAGTGGTAG AACAAGCAGG AGCCATCATA CTTGAAGACG GACAAGAACT TGATTCTTGG 
TATGTTATTT TAAATGGCAC TGTGGAGATC AGCTATCCAG ATGGGAAAAC TGAAAATCTC 
TGTATGGGAA ATAGTTTTGG GATTACTCCT TCTCTGGAAA AACAGTATAT GAATGGAGTG 
GTTAGAACCA AAGTAGATGA CTGTCAGTTT GTATGCATAG CCCAGCAAGA TTATTGGCGC 
ATTCTGAACC ACGTGGAAAA AAACACTCAT AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAGGAGC ACAGAGAGCT GGATCGCAGT GGAACCAGGA AAGGACACAT AGTTATTAAG 
GCAACACCTG AGCGACTCAT CACACATTTA ATAGAAGAGC ATTCCATCGT TGATCCAACA 
TACATAGAAG ACTTCCTTTT AACATATAGA ACATTTCTCT CGAGCCCCTT GGAAGTTGGG 
GATAAGCTCT TGGAGTGGTT TAAAATAGAT AGCCTCAGGG ATAAGGTTAC CAGAGTGGTC 
TTATTGTGGG TAAACAATCA TTTTAATGAT TTTGAAGGAG ATCCTGCTAT GACACGTTTT 
CTGGAAGAAT TTGAAAAAAA CTTGGAAAAT ACGAAAATGA AGGGGCACCT CAGGTTGTTG 
AATATTGCTT GTGCTGCCAA GGCAAAATGG AGGCAGATTA CCCTGCAGAA GGCTTCCAGA 
GACTCGCCTC TGTATTTCAG CCTCCTGGGA GGAAGTGAGA AGGGTTTTGG TATATTTGTA 
GAGACTGTAG AAACAGGCAG CAAAGCAGCA GAAGTTGGGC TGAAGCGTGG CGATCAGATA 
ATGGAAGTAA ATGGGCAGAA CTTTGAGAAC ATTACTTTTG TGAAAGCCCT TGAAATCTTG 
AGGAACAATA CTCATCTTTC CATTACAGTA AAGACAAACA TTTTTGTGTT TAAGGAACTG 
CTTAGCCGGA TAGGCCAGGA GAAAAACGGA GTCCCACATA TTCCAAAAAT TGCAGAAAAG 
AAAAATAACC GTTACTCCAT CCCAGATATG CCAGGAGACA TGGAACAGAT ATTTCCACGT 
GAGAAAGGAA ACAAGAAAAT GAAAGCAAAC ACTGTATCCG GTGGAAGAAA TAGGATCAAG 
AAAATTTTAG ACAAGACTCG TTTCAGCATT TTGCCCCCAA AACTATTTAG TGATGGCAGT 
GTAAGTCAAT CCCAGGATGA CAGCATTGTT GGAACAAGGC AGTGCAGACA CAGCCTGGCC 
ATCATGCCTG TCCCTGGAAC ACTTTCATCC AGCAGCCCGG ACCTTTTGCA GCCTACAACC 
AGCATTCTTG ATTTTTCTAA TCCTTCAGAT ATTCCAGATC AAGTTATTAG AGTTTTCAAA 
GCAGATCAAC AGAGTTGTTA CATTATCATC AGCAAAGACA CTACAGCGAA AGAAGTTGTG 
AGTCATGCTG TGCATGAATT CAACTTGACG GGTGCGTCAG AGACCTACTC TCTCTGCGAG 
GTGTCTGTTA GTCCTGAGGG AGTCATCAAA CAAAGAAGGC TTCCAGATCA GTTCTCAAAA 
TTAGCAGACA GGATTCAGCT CAATGGAAGG TATTATCTGA AAAACAATAT GGAAACAGAG 
ACCTTATGCT CAGATGAAGA TGCCCAAGAG CTGCTCAGAG AGAGTCAAAT ATCTCTTCTT 
CAGCTGAGCA CCATTGAAGT GGCAACTCAG CTTTCAATGA GAGACTTTGA ATTATTTCGC 
AACATTGAGC CTACTGAATA CATTGATGAC CTTTTTAAGC TGGACTCAAA AACAGGAAAT 
GCTCACCTGA AACAATTTGA GGATGTCATT AACCAAGAGA CATTTTGGGT TGCCACAGAA 
ATTTTATTAG AGCCCAACCA GCTCAAAAGA ATGAAAATTA TTAAACATTT CATTAAAATT 
GCCCTCCACT GTCGAGAATG CAAGAACTTC AATTCCATGT TTGCAGTTAT AAGTGGGCTA 
AATCTGGCAT CGGTAGCACG ACTCAGAGGT ACCTGGGAAA AATTACCTAG CAAGTATGAA 
AAGCATTTCA GAGACCTTCA GGATCTTTTT GATCCATCTA GAAACATGGC AAAATACCGC 
AATATTCTTA GCAGTCAGAG CATGCAGCCT CCAATTATTC CACTCTTTCC CGTTGTCAAG 
AAGGATATTA CATTTCTACA TGAAGGAAAT GATTCCAAAG TGGATGGCCT GGTAAATTTT 
GAGAAACTCA GAATGATTGC CAAAGAAATC CGGCAAGTTA TCAGAATGAC CTCTGCTAAC 
ATGGATCCGG CTGTAATGTT CAGGCAAAGG TCTCTGAGCC AGGGCAGCAC AAACTCAAAT 
ATGCTCGATG TTCAAGGAGG TGCTCACAAA AAACGTGCCC GACGCAGTTC GCTCCTTAAT 
GCCAAGAAGC TATATGAGGA TGCCCAGATG GCCAGAAAGG TGAAGCAGTA CCTGTCCAAC 
CTGAATGTAG AAACTGATGA GGAAAAATTC CAGATCATGT CACTTCAGTG CGAGCCTGCT 
TATAGCACTT TGACAAAGAA TTTAAGTGAG AGAAGAGGTG GGAAATCCTC AGAAATGTCT 
CCAATACCCT TGAGGTCAGT TGGCCAAACC ACAAAAGTTC AGCAGCATCA GCAAAACAGA 
ATGAGCCAAG TTCTTCAAGT TCCTGCTGTT AATTTGTATC CCATTAGAAA AAAGGGACCA 
GCAAAGGACC ACGTCACTTT TAATACAGGT TCTCCTCAGA AGTCATTAAG TTTGTCGGAG 
GAAGTAAGTA AGAAACCAGC AGAAGATGCT GTGTCTATGG CATCTTCTTT GCACTCCAGT 
CCTCTTGCAT CTCCTCAGGG CTCTCCACGC AAAGTTGGTA ACATCCAGAG GTCTGATAAT 
TGCAGTCAGA TGAATCTTTC TGGATCCTCT TCATCGCTTG CCAGTGAAAC CAGTAACAAG 
AACAGTGGGC AACGCAGCTA TGGAATAGGC TATGCTCTCA TACCTTCTGC CAAGTCAGAT 
AATTTCTCAG ACTCCAGCCA CAGCGAGATC TCCTCACGGT CCAGCATAGT GAGCAACTGC 
TCTGTCGACT CCATGTCTGC AGCCCTCCAG GACGAGAGGG GTTTGCCCCA GTCTCTTAGT 
GTCGTGGATT CAGTGGGAAC AGAAAGAAAA GAACATCCTC CACCATTGGC TGAGTACAGT 
CAGCCTTGCC CAGGTTGGCC CCTCCCTAGA CCGGCTATAA TCAGAGGATT GGCTGTCACA 
TCGTCCATGA GCAATGAGGA GATCTCCCAT GAACATATTA CAATAGAAGC CGCAGACAGT 
GGCCGTGGAA GCTGGACTTC ATGTTCAAGT AGTTCTCACG ATAACTTCCA GAATATCCAA 
ATTCAGAAAA GCTGGGATCT CTTGAATTCT TACCGCCACA CTCACTTGGA TGGACCTATT 
GCTGAAGTAG AACCCACAAA CTGTTACCCT GAAGAGGCGT ATTTGTATTC CGAAGCCTTT 
TCCAAAAATA ATAGGCAGTC CAAAACCAGC GTGGAATTGG ATCAGTCTCG GCAGAGCTGG 
GCCTCCTCTA GCTCTCTGTC AGACACGTAT GAAACAAACT ATGGGACGAT TAAACGGAGA 
GGGCTGGAGA ACTCAACAAT GGAGCAGACT GAGATTTTGG ACTCTAAACC AGGCACTGAT 
GCAACTTACA AAACTGTTAC TTCTAGTACA GAAAAGGGCC TAATAGTGTA CTGTGTCACC 
TCACCTAAGA AGGACGATAG GTATAGGGAG CCACCGCCCA CCCCTCCGGG ATATATGGGG 
ATTTCTTTAG CGGACATAAA GGAAGGATCG CCCCACCACC CACACTTAAA ACCTCCAGAC 
TATAGTGTGG CAGTGCAGAG GTCAAAGATG CTACATAGTG ACCTCTCTAG ACTTTCATCA 
GCTTCTCTCA GTAGTGAACC AGTGGCCTGT ATTTCATCGA AGATGCCTCA GTGGCATACC 
CGGCAGCCGT CCCATCCTAA ACTAACAGAT ATGACTGACG CAGATAGTGA AGAGGATGAA 
GATGAACAAG TATCAGCAGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_019478367.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019333912.1 Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X6, mRNA.

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CloneID	OAl76330
Clone ID Related Accession (Same CDS sequence)	XM_019478367.1
Accession Version	XM_019478367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	rap guanine nucleotide exchange factor 6 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017713851.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCCG CCGTGGACCC CAAGGTCCAA CAGGCCCTGC GGAAGAAGCC CCCGGAGCGG 
AGCCGCGAGG ACTTAAATAT TATCTATACT TATCTTCATG GAATGGACAT ATTGTCTCAT 
CTCAGGGAAC ATCAGCTAAG AATAATGTCT TCAAGTGCCC GCTATGAAAG ATACAAGGGA 
AACCAAGTTC TCTTTTGCTC AGAAACAATC GCCAGATGTT GGTACATTCT TCTTTCCGGA 
TCTGTGCTTA TGAAGGACTC CATGTTTTTA CCACCATGCA GTTTTGGCAA GCAGTCTGGA 
GGAAAGCGAG GATGCGAGTG TATTGTACTG GAGCCTTCTG AAATGATTGT GGTGGAGAAC 
TCCAAAGACA ACGAAGAAAA TATCTTGCAA AGAGAAGTTC CCATCAGACA GTCTCGGCGA 
AGGTTCAGGA AGATTAATCA ACGGGGAGAA CGCCAGACCA TCACTGACCA TGTGGATGCT 
AGCAGTTATC TTTCGCTTCC AGCAGATCTT ACCAAGATGC AAATTGCAGA CAATCCACAT 
CCACAGGTGA CTCATGTCCC TTCCAGCCAG TCTGGATGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGCT TGTCTGATAT ATATCAGGCT ACAGAAAGTG AGATGGGAGA TGTAGATTTA 
ACAGGCCTCC CAGAAGCACC TGTGGACTCT GAAGATGAAG AGGAGGAGGA AGATGAAGAT 
ATAGACAGAA CTTCAGATCC ATTATTGGGG CGAGACGTTG TACGAGAGTG CCTTGAAAAA 
GAACCTGCAG ATAAAACGGA TGATGACATT GAGCAATTAC TGGAATTTAT GCATCAGCTC 
CCAGCTTTTG CAAACATGAC CATGTCTGTG AGAAGAGAGC TTTGTTCAGT TATGATATTT 
GAAGTGGTAG AACAAGCAGG AGCCATCATA CTTGAAGACG GACAAGAACT TGATTCTTGG 
TATGTTATTT TAAATGGCAC TGTGGAGATC AGCTATCCAG ATGGGAAAAC TGAAAATCTC 
TGTATGGGAA ATAGTTTTGG GATTACTCCT TCTCTGGAAA AACAGTATAT GAATGGAGTG 
GTTAGAACCA AAGTAGATGA CTGTCAGTTT GTATGCATAG CCCAGCAAGA TTATTGGCGC 
ATTCTGAACC ACGTGGAAAA AAACACTCAT AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAGGAGC ACAGAGAGCT GGATCGCAGT GGAACCAGGA AAGGACACAT AGTTATTAAG 
GCAACACCTG AGCGACTCAT CACACATTTA ATAGAAGAGC ATTCCATCGT TGATCCAACA 
TACATAGAAG ACTTCCTTTT AACATATAGA ACATTTCTCT CGAGCCCCTT GGAAGTTGGG 
GATAAGCTCT TGGAGTGGTT TAAAATAGAT AGCCTCAGGG ATAAGGTTAC CAGAGTGGTC 
TTATTGTGGG TAAACAATCA TTTTAATGAT TTTGAAGGAG ATCCTGCTAT GACACGTTTT 
CTGGAAGAAT TTGAAAAAAA CTTGGAAAAT ACGAAAATGA AGGGGCACCT CAGGTTGTTG 
AATATTGCTT GTGCTGCCAA GGCAAAATGG AGGCAGATTA CCCTGCAGAA GGCTTCCAGA 
GACTCGCCTC TGTATTTCAG CCTCCTGGGA GGAAGTGAGA AGGGTTTTGG TATATTTGTA 
GAGACTGTAG AAACAGGCAG CAAAGCAGCA GAAGTTGGGC TGAAGCGTGG CGATCAGATA 
ATGGAAGTAA ATGGGCAGAA CTTTGAGAAC ATTACTTTTG TGAAAGCCCT TGAAATCTTG 
AGGAACAATA CTCATCTTTC CATTACAGTA AAGACAAACA TTTTTGTGTT TAAGGAACTG 
CTTAGCCGGA TAGGCCAGGA GAAAAACGGA GTCCCACATA TTCCAAAAAT TGCAGAAAAG 
AAAAATAACC GTTACTCCAT CCCAGATATG CCAGGAGACA TGGAACAGAT ATTTCCACGT 
GAGAAAGGAA ACAAGAAAAT GAAAGCAAAC ACTGTATCCG GTGGAAGAAA TAGGATCAAG 
AAAATTTTAG ACAAGACTCG TTTCAGCATT TTGCCCCCAA AACTATTTAG TGATGGCAGT 
GTAAGTCAAT CCCAGGATGA CAGCATTGTT GGAACAAGGC AGTGCAGACA CAGCCTGGCC 
ATCATGCCTG TCCCTGGAAC ACTTTCATCC AGCAGCCCGG ACCTTTTGCA GCCTACAACC 
AGCATTCTTG ATTTTTCTAA TCCTTCAGCG GTCGGTTTTT ACTATATTCC AGATCAAGTT 
ATTAGAGTTT TCAAAGCAGA TCAACAGAGT TGTTACATTA TCATCAGCAA AGACACTACA 
GCGAAAGAAG TTGTGAGTCA TGCTGTGCAT GAATTCAACT TGACGGGTGC GTCAGAGACC 
TACTCTCTCT GCGAGGTGTC TGTTAGTCCT GAGGGAGTCA TCAAACAAAG AAGGCTTCCA 
GATCAGTTCT CAAAATTAGC AGACAGGATT CAGCTCAATG GAAGGTATTA TCTGAAAAAC 
AATATGGAAA CAGAGACCTT ATGCTCAGAT GAAGATGCCC AAGAGCTGCT CAGAGAGAGT 
CAAATATCTC TTCTTCAGCT GAGCACCATT GAAGTGGCAA CTCAGCTTTC AATGAGAGAC 
TTTGAATTAT TTCGCAACAT TGAGCCTACT GAATACATTG ATGACCTTTT TAAGCTGGAC 
TCAAAAACAG GAAATGCTCA CCTGAAACAA TTTGAGGATG TCATTAACCA AGAGACATTT 
TGGGTTGCCA CAGAAATTTT ATTAGAGCCC AACCAGCTCA AAAGAATGAA AATTATTAAA 
CATTTCATTA AAATTGCCCT CCACTGTCGA GAATGCAAGA ACTTCAATTC CATGTTTGCA 
GTTATAAGTG GGCTAAATCT GGCATCGGTA GCACGACTCA GAGGTACCTG GGAAAAATTA 
CCTAGCAAGT ATGAAAAGCA TTTCAGAGAC CTTCAGGATC TTTTTGATCC ATCTAGAAAC 
ATGGCAAAAT ACCGCAATAT TCTTAGCAGT CAGAGCATGC AGCCTCCAAT TATTCCACTC 
TTTCCCGTTG TCAAGAAGGA TATTACATTT CTACATGAAG GAAATGATTC CAAAGTGGAT 
GGCCTGGTAA ATTTTGAGAA ACTCAGAATG ATTGCCAAAG AAATCCGGCA AGTTATCAGA 
ATGACCTCTG CTAACATGGA TCCGGCTGTA ATGTTCAGGC AAAGGAAGAA GAGGTGGAGG 
AGTTTGGGGT CTCTGAGCCA GGGCAGCACA AACTCAAATA TGCTCGATGT TCAAGGAGGT 
GCTCACAAAA AACGTGCCCG ACGCAGTTCG CTCCTTAATG CCAAGAAGCT ATATGAGGAT 
GCCCAGATGG CCAGAAAGGT GAAGCAGTAC CTGTCCAACC TGAATGTAGA AACTGATGAG 
GAAAAATTCC AGATCATGTC ACTTCAGTGC GAGCCTGCTT ATAGCACTTT GACAAAGAAT 
TTAAGTGAGA GAAGAGGTGG GAAATCCTCA GAAATGTCTC CAATACCCTT GAGGTCAGTT 
GGCCAAACCA CAAAAGTTCA GCAGCATCAG CAAAACAGAA TGAGCCAAGT TCTTCAAGTT 
CCTGCTGTTA ATTTGTATCC CATTAGAAAA AAGGGACCAG CAAAGGACCA CGTCACTTTT 
AATACAGGTT CTCCTCAGAA GTCATTAAGT TTGTCGGAGG AAGTAAGTAA GAAACCAGCA 
GAAGATGCTG TGTCTATGGC ATCTTCTTTG CACTCCAGTC CTCTTGCATC TCCTCAGGGC 
TCTCCACGCA AAGTTGGTAA CATCCAGAGG TCTGATAATT GCAGTCAGAT GAATCTTTCT 
GGATCCTCTT CATCGCTTGC CAGTGAAACC AGTAACAAGA ACAGTGGGCA ACGCAGCTAT 
GGAATAGGCT ATGCTCTCAT ACCTTCTGCC AAGTCAGATA ATTTCTCAGA CTCCAGCCAC 
AGCGAGATCT CCTCACGGTC CAGCATAGTG AGCAACTGCT CTGTCGACTC CATGTCTGCA 
GCCCTCCAGG ACGAGAGGGG TTTGCCCCAG TCTCTTAGTG TCGTGGATTC AGTGGGAACA 
GAAAGAAAAG AACATCCTCC ACCATTGGCT GAGTACAGTC AGCCTTGCCC AGGTTGGCCC 
CTCCCTAGAC CGGCTATAAT CAGAGGATTG GCTGTCACAT CGTCCATGAG CAATGAGGAG 
ATCTCCCATG AACATATTAC AATAGAAGCC GCAGACAGTG GCCGTGGAAG CTGGACTTCA 
TGTTCAAGTA GTTCTCACGA TAACTTCCAG AATATCCAAA TTCAGAAAAG CTGGGATCTC 
TTGAATTCTT ACCGCCACAC TCACTTGGAT GGACCTATTG CTGAAGTAGA ACCCACAAAC 
TGTTACCCTG AAGAGGCGTA TTTGTATTCC GAAGCCTTTT CCAAAAATAA TAGGCAGTCC 
AAAACCAGCG TGGAATTGGA TCAGTCTCGG CAGAGCTGGG CCTCCTCTAG CTCTCTGTCA 
GACACGTATG AAACAAACTA TGGGACGATT AAACGGAGAG GGCTGGAGAA CTCAACAATG 
GAGCAGACTG AGATTTTGGA CTCTAAACCA GGCACTGATG CAACTTACAA AACTGTTACT 
TCTAGTACAG AAAAGGGCCT AATAGAAGAT GAACAAGTAT CAGCAGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019333912.1
CDS	269..4939
Translation	MGSAVDPKVQQALRKKPPERSREDLNIIYTYLHGMDILSHLREHQLRIMSSSARYERYKGNQVLFCSETIARCWYILLSGSVLMKDSMFLPPCSFGKQSGGKRGCECIVLEPSEMIVVENSKDNEENILQREVPIRQSRRRFRKINQRGERQTITDHVDASSYLSLPADLTKMQIADNPHPQVTHVPSSQSGCSIASDSGSSSLSDIYQATESEMGDVDLTGLPEAPVDSEDEEEEEDEDIDRTSDPLLGRDVVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISYPDGKTENLCMGNSFGITPSLEKQYMNGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVKEHRELDRSGTRKGHIVIKATPERLITHLIEEHSIVDPTYIEDFLLTYRTFLSSPLEVGDKLLEWFKIDSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFEKNLENTKMKGHLRLLNIACAAKAKWRQITLQKASRDSPLYFSLLGGSEKGFGIFVETVETGSKAAEVGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLSITVKTNIFVFKELLSRIGQEKNGVPHIPKIAEKKNNRYSIPDMPGDMEQIFPREKGNKKMKANTVSGGRNRIKKILDKTRFSILPPKLFSDGSVSQSQDDSIVGTRQCRHSLAIMPVPGTLSSSSPDLLQPTTSILDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVSHAVHEFNLTGASETYSLCEVSVSPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTIEVATQLSMRDFELFRNIEPTEYIDDLFKLDSKTGNAHLKQFEDVINQETFWVATEILLEPNQLKRMKIIKHFIKIALHCRECKNFNSMFAVISGLNLASVARLRGTWEKLPSKYEKHFRDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDITFLHEGNDSKVDGLVNFEKLRMIAKEIRQVIRMTSANMDPAVMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSNLNVETDEEKFQIMSLQCEPAYSTLTKNLSERRGGKSSEMSPIPLRSVGQTTKVQQHQQNRMSQVLQVPAVNLYPIRKKGPAKDHVTFNTGSPQKSLSLSEEVSKKPAEDAVSMASSLHSSPLASPQGSPRKVGNIQRSDNCSQMNLSGSSSSLASETSNKNSGQRSYGIGYALIPSAKSDNFSDSSHSEISSRSSIVSNCSVDSMSAALQDERGLPQSLSVVDSVGTERKEHPPPLAEYSQPCPGWPLPRPAIIRGLAVTSSMSNEEISHEHITIEAADSGRGSWTSCSSSSHDNFQNIQIQKSWDLLNSYRHTHLDGPIAEVEPTNCYPEEAYLYSEAFSKNNRQSKTSVELDQSRQSWASSSSLSDTYETNYGTIKRRGLENSTMEQTEILDSKPGTDATYKTVTSSTEKGLIEDEQVSAV

Target ORF information:

RefSeq Version	XM_019478367.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019478367.1

ORF Insert Sequence:

ATGGGCTCCG CCGTGGACCC CAAGGTCCAA CAGGCCCTGC GGAAGAAGCC CCCGGAGCGG 
AGCCGCGAGG ACTTAAATAT TATCTATACT TATCTTCATG GAATGGACAT ATTGTCTCAT 
CTCAGGGAAC ATCAGCTAAG AATAATGTCT TCAAGTGCCC GCTATGAAAG ATACAAGGGA 
AACCAAGTTC TCTTTTGCTC AGAAACAATC GCCAGATGTT GGTACATTCT TCTTTCCGGA 
TCTGTGCTTA TGAAGGACTC CATGTTTTTA CCACCATGCA GTTTTGGCAA GCAGTCTGGA 
GGAAAGCGAG GATGCGAGTG TATTGTACTG GAGCCTTCTG AAATGATTGT GGTGGAGAAC 
TCCAAAGACA ACGAAGAAAA TATCTTGCAA AGAGAAGTTC CCATCAGACA GTCTCGGCGA 
AGGTTCAGGA AGATTAATCA ACGGGGAGAA CGCCAGACCA TCACTGACCA TGTGGATGCT 
AGCAGTTATC TTTCGCTTCC AGCAGATCTT ACCAAGATGC AAATTGCAGA CAATCCACAT 
CCACAGGTGA CTCATGTCCC TTCCAGCCAG TCTGGATGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGCT TGTCTGATAT ATATCAGGCT ACAGAAAGTG AGATGGGAGA TGTAGATTTA 
ACAGGCCTCC CAGAAGCACC TGTGGACTCT GAAGATGAAG AGGAGGAGGA AGATGAAGAT 
ATAGACAGAA CTTCAGATCC ATTATTGGGG CGAGACGTTG TACGAGAGTG CCTTGAAAAA 
GAACCTGCAG ATAAAACGGA TGATGACATT GAGCAATTAC TGGAATTTAT GCATCAGCTC 
CCAGCTTTTG CAAACATGAC CATGTCTGTG AGAAGAGAGC TTTGTTCAGT TATGATATTT 
GAAGTGGTAG AACAAGCAGG AGCCATCATA CTTGAAGACG GACAAGAACT TGATTCTTGG 
TATGTTATTT TAAATGGCAC TGTGGAGATC AGCTATCCAG ATGGGAAAAC TGAAAATCTC 
TGTATGGGAA ATAGTTTTGG GATTACTCCT TCTCTGGAAA AACAGTATAT GAATGGAGTG 
GTTAGAACCA AAGTAGATGA CTGTCAGTTT GTATGCATAG CCCAGCAAGA TTATTGGCGC 
ATTCTGAACC ACGTGGAAAA AAACACTCAT AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAGGAGC ACAGAGAGCT GGATCGCAGT GGAACCAGGA AAGGACACAT AGTTATTAAG 
GCAACACCTG AGCGACTCAT CACACATTTA ATAGAAGAGC ATTCCATCGT TGATCCAACA 
TACATAGAAG ACTTCCTTTT AACATATAGA ACATTTCTCT CGAGCCCCTT GGAAGTTGGG 
GATAAGCTCT TGGAGTGGTT TAAAATAGAT AGCCTCAGGG ATAAGGTTAC CAGAGTGGTC 
TTATTGTGGG TAAACAATCA TTTTAATGAT TTTGAAGGAG ATCCTGCTAT GACACGTTTT 
CTGGAAGAAT TTGAAAAAAA CTTGGAAAAT ACGAAAATGA AGGGGCACCT CAGGTTGTTG 
AATATTGCTT GTGCTGCCAA GGCAAAATGG AGGCAGATTA CCCTGCAGAA GGCTTCCAGA 
GACTCGCCTC TGTATTTCAG CCTCCTGGGA GGAAGTGAGA AGGGTTTTGG TATATTTGTA 
GAGACTGTAG AAACAGGCAG CAAAGCAGCA GAAGTTGGGC TGAAGCGTGG CGATCAGATA 
ATGGAAGTAA ATGGGCAGAA CTTTGAGAAC ATTACTTTTG TGAAAGCCCT TGAAATCTTG 
AGGAACAATA CTCATCTTTC CATTACAGTA AAGACAAACA TTTTTGTGTT TAAGGAACTG 
CTTAGCCGGA TAGGCCAGGA GAAAAACGGA GTCCCACATA TTCCAAAAAT TGCAGAAAAG 
AAAAATAACC GTTACTCCAT CCCAGATATG CCAGGAGACA TGGAACAGAT ATTTCCACGT 
GAGAAAGGAA ACAAGAAAAT GAAAGCAAAC ACTGTATCCG GTGGAAGAAA TAGGATCAAG 
AAAATTTTAG ACAAGACTCG TTTCAGCATT TTGCCCCCAA AACTATTTAG TGATGGCAGT 
GTAAGTCAAT CCCAGGATGA CAGCATTGTT GGAACAAGGC AGTGCAGACA CAGCCTGGCC 
ATCATGCCTG TCCCTGGAAC ACTTTCATCC AGCAGCCCGG ACCTTTTGCA GCCTACAACC 
AGCATTCTTG ATTTTTCTAA TCCTTCAGCG GTCGGTTTTT ACTATATTCC AGATCAAGTT 
ATTAGAGTTT TCAAAGCAGA TCAACAGAGT TGTTACATTA TCATCAGCAA AGACACTACA 
GCGAAAGAAG TTGTGAGTCA TGCTGTGCAT GAATTCAACT TGACGGGTGC GTCAGAGACC 
TACTCTCTCT GCGAGGTGTC TGTTAGTCCT GAGGGAGTCA TCAAACAAAG AAGGCTTCCA 
GATCAGTTCT CAAAATTAGC AGACAGGATT CAGCTCAATG GAAGGTATTA TCTGAAAAAC 
AATATGGAAA CAGAGACCTT ATGCTCAGAT GAAGATGCCC AAGAGCTGCT CAGAGAGAGT 
CAAATATCTC TTCTTCAGCT GAGCACCATT GAAGTGGCAA CTCAGCTTTC AATGAGAGAC 
TTTGAATTAT TTCGCAACAT TGAGCCTACT GAATACATTG ATGACCTTTT TAAGCTGGAC 
TCAAAAACAG GAAATGCTCA CCTGAAACAA TTTGAGGATG TCATTAACCA AGAGACATTT 
TGGGTTGCCA CAGAAATTTT ATTAGAGCCC AACCAGCTCA AAAGAATGAA AATTATTAAA 
CATTTCATTA AAATTGCCCT CCACTGTCGA GAATGCAAGA ACTTCAATTC CATGTTTGCA 
GTTATAAGTG GGCTAAATCT GGCATCGGTA GCACGACTCA GAGGTACCTG GGAAAAATTA 
CCTAGCAAGT ATGAAAAGCA TTTCAGAGAC CTTCAGGATC TTTTTGATCC ATCTAGAAAC 
ATGGCAAAAT ACCGCAATAT TCTTAGCAGT CAGAGCATGC AGCCTCCAAT TATTCCACTC 
TTTCCCGTTG TCAAGAAGGA TATTACATTT CTACATGAAG GAAATGATTC CAAAGTGGAT 
GGCCTGGTAA ATTTTGAGAA ACTCAGAATG ATTGCCAAAG AAATCCGGCA AGTTATCAGA 
ATGACCTCTG CTAACATGGA TCCGGCTGTA ATGTTCAGGC AAAGGAAGAA GAGGTGGAGG 
AGTTTGGGGT CTCTGAGCCA GGGCAGCACA AACTCAAATA TGCTCGATGT TCAAGGAGGT 
GCTCACAAAA AACGTGCCCG ACGCAGTTCG CTCCTTAATG CCAAGAAGCT ATATGAGGAT 
GCCCAGATGG CCAGAAAGGT GAAGCAGTAC CTGTCCAACC TGAATGTAGA AACTGATGAG 
GAAAAATTCC AGATCATGTC ACTTCAGTGC GAGCCTGCTT ATAGCACTTT GACAAAGAAT 
TTAAGTGAGA GAAGAGGTGG GAAATCCTCA GAAATGTCTC CAATACCCTT GAGGTCAGTT 
GGCCAAACCA CAAAAGTTCA GCAGCATCAG CAAAACAGAA TGAGCCAAGT TCTTCAAGTT 
CCTGCTGTTA ATTTGTATCC CATTAGAAAA AAGGGACCAG CAAAGGACCA CGTCACTTTT 
AATACAGGTT CTCCTCAGAA GTCATTAAGT TTGTCGGAGG AAGTAAGTAA GAAACCAGCA 
GAAGATGCTG TGTCTATGGC ATCTTCTTTG CACTCCAGTC CTCTTGCATC TCCTCAGGGC 
TCTCCACGCA AAGTTGGTAA CATCCAGAGG TCTGATAATT GCAGTCAGAT GAATCTTTCT 
GGATCCTCTT CATCGCTTGC CAGTGAAACC AGTAACAAGA ACAGTGGGCA ACGCAGCTAT 
GGAATAGGCT ATGCTCTCAT ACCTTCTGCC AAGTCAGATA ATTTCTCAGA CTCCAGCCAC 
AGCGAGATCT CCTCACGGTC CAGCATAGTG AGCAACTGCT CTGTCGACTC CATGTCTGCA 
GCCCTCCAGG ACGAGAGGGG TTTGCCCCAG TCTCTTAGTG TCGTGGATTC AGTGGGAACA 
GAAAGAAAAG AACATCCTCC ACCATTGGCT GAGTACAGTC AGCCTTGCCC AGGTTGGCCC 
CTCCCTAGAC CGGCTATAAT CAGAGGATTG GCTGTCACAT CGTCCATGAG CAATGAGGAG 
ATCTCCCATG AACATATTAC AATAGAAGCC GCAGACAGTG GCCGTGGAAG CTGGACTTCA 
TGTTCAAGTA GTTCTCACGA TAACTTCCAG AATATCCAAA TTCAGAAAAG CTGGGATCTC 
TTGAATTCTT ACCGCCACAC TCACTTGGAT GGACCTATTG CTGAAGTAGA ACCCACAAAC 
TGTTACCCTG AAGAGGCGTA TTTGTATTCC GAAGCCTTTT CCAAAAATAA TAGGCAGTCC 
AAAACCAGCG TGGAATTGGA TCAGTCTCGG CAGAGCTGGG CCTCCTCTAG CTCTCTGTCA 
GACACGTATG AAACAAACTA TGGGACGATT AAACGGAGAG GGCTGGAGAA CTCAACAATG 
GAGCAGACTG AGATTTTGGA CTCTAAACCA GGCACTGATG CAACTTACAA AACTGTTACT 
TCTAGTACAG AAAAGGGCCT AATAGAAGAT GAACAAGTAT CAGCAGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_019478368.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019333913.1 Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OAl76331
Clone ID Related Accession (Same CDS sequence)	XM_019478368.1
Accession Version	XM_019478368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	rap guanine nucleotide exchange factor 6 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017713851.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTTC TTGTTCGCTG TTACGCCAAC TGTCTGCAGC CATGGGCCTC CAAACTTCCA 
GCAGATCTTA CCAAGATGCA AATTGCAGAC AATCCACATC CACAGGTGAC TCATGTCCCT 
TCCAGCCAGT CTGGATGTAG CATTGCCAGT GACTCTGGGA GCAGCAGCTT GTCTGATATA 
TATCAGGCTA CAGAAAGTGA GATGGGAGAT GTAGATTTAA CAGGCCTCCC AGAAGCACCT 
GTGGACTCTG AAGATGAAGA GGAGGAGGAA GATGAAGATA TAGACAGAAC TTCAGATCCA 
TTATTGGGGC GAGACGTTGT ACGAGAGTGC CTTGAAAAAG AACCTGCAGA TAAAACGGAT 
GATGACATTG AGCAATTACT GGAATTTATG CATCAGCTCC CAGCTTTTGC AAACATGACC 
ATGTCTGTGA GAAGAGAGCT TTGTTCAGTT ATGATATTTG AAGTGGTAGA ACAAGCAGGA 
GCCATCATAC TTGAAGACGG ACAAGAACTT GATTCTTGGT ATGTTATTTT AAATGGCACT 
GTGGAGATCA GCTATCCAGA TGGGAAAACT GAAAATCTCT GTATGGGAAA TAGTTTTGGG 
ATTACTCCTT CTCTGGAAAA ACAGTATATG AATGGAGTGG TTAGAACCAA AGTAGATGAC 
TGTCAGTTTG TATGCATAGC CCAGCAAGAT TATTGGCGCA TTCTGAACCA CGTGGAAAAA 
AACACTCATA AAGTTGAGGA AGAAGGAGAA ATTGTTATGG TAAAGGAGCA CAGAGAGCTG 
GATCGCAGTG GAACCAGGAA AGGACACATA GTTATTAAGG CAACACCTGA GCGACTCATC 
ACACATTTAA TAGAAGAGCA TTCCATCGTT GATCCAACAT ACATAGAAGA CTTCCTTTTA 
ACATATAGAA CATTTCTCTC GAGCCCCTTG GAAGTTGGGG ATAAGCTCTT GGAGTGGTTT 
AAAATAGATA GCCTCAGGGA TAAGGTTACC AGAGTGGTCT TATTGTGGGT AAACAATCAT 
TTTAATGATT TTGAAGGAGA TCCTGCTATG ACACGTTTTC TGGAAGAATT TGAAAAAAAC 
TTGGAAAATA CGAAAATGAA GGGGCACCTC AGGTTGTTGA ATATTGCTTG TGCTGCCAAG 
GCAAAATGGA GGCAGATTAC CCTGCAGAAG GCTTCCAGAG ACTCGCCTCT GTATTTCAGC 
CTCCTGGGAG GAAGTGAGAA GGGTTTTGGT ATATTTGTAG AGACTGTAGA AACAGGCAGC 
AAAGCAGCAG AAGTTGGGCT GAAGCGTGGC GATCAGATAA TGGAAGTAAA TGGGCAGAAC 
TTTGAGAACA TTACTTTTGT GAAAGCCCTT GAAATCTTGA GGAACAATAC TCATCTTTCC 
ATTACAGTAA AGACAAACAT TTTTGTGTTT AAGGAACTGC TTAGCCGGAT AGGCCAGGAG 
AAAAACGGAG TCCCACATAT TCCAAAAATT GCAGAAAAGA AAAATAACCG TTACTCCATC 
CCAGATATGC CAGGAGACAT GGAACAGATA TTTCCACGTG AGAAAGGAAA CAAGAAAATG 
AAAGCAAACA CTGTATCCGG TGGAAGAAAT AGGATCAAGA AAATTTTAGA CAAGACTCGT 
TTCAGCATTT TGCCCCCAAA ACTATTTAGT GATGGCAGTG TAAGTCAATC CCAGGATGAC 
AGCATTGTTG GAACAAGGCA GTGCAGACAC AGCCTGGCCA TCATGCCTGT CCCTGGAACA 
CTTTCATCCA GCAGCCCGGA CCTTTTGCAG CCTACAACCA GCATTCTTGA TTTTTCTAAT 
CCTTCAGCGG TCGGTTTTTA CTATATTCCA GATCAAGTTA TTAGAGTTTT CAAAGCAGAT 
CAACAGAGTT GTTACATTAT CATCAGCAAA GACACTACAG CGAAAGAAGT TGTGAGTCAT 
GCTGTGCATG AATTCAACTT GACGGGTGCG TCAGAGACCT ACTCTCTCTG CGAGGTGTCT 
GTTAGTCCTG AGGGAGTCAT CAAACAAAGA AGGCTTCCAG ATCAGTTCTC AAAATTAGCA 
GACAGGATTC AGCTCAATGG AAGGTATTAT CTGAAAAACA ATATGGAAAC AGAGACCTTA 
TGCTCAGATG AAGATGCCCA AGAGCTGCTC AGAGAGAGTC AAATATCTCT TCTTCAGCTG 
AGCACCATTG AAGTGGCAAC TCAGCTTTCA ATGAGAGACT TTGAATTATT TCGCAACATT 
GAGCCTACTG AATACATTGA TGACCTTTTT AAGCTGGACT CAAAAACAGG AAATGCTCAC 
CTGAAACAAT TTGAGGATGT CATTAACCAA GAGACATTTT GGGTTGCCAC AGAAATTTTA 
TTAGAGCCCA ACCAGCTCAA AAGAATGAAA ATTATTAAAC ATTTCATTAA AATTGCCCTC 
CACTGTCGAG AATGCAAGAA CTTCAATTCC ATGTTTGCAG TTATAAGTGG GCTAAATCTG 
GCATCGGTAG CACGACTCAG AGGTACCTGG GAAAAATTAC CTAGCAAGTA TGAAAAGCAT 
TTCAGAGACC TTCAGGATCT TTTTGATCCA TCTAGAAACA TGGCAAAATA CCGCAATATT 
CTTAGCAGTC AGAGCATGCA GCCTCCAATT ATTCCACTCT TTCCCGTTGT CAAGAAGGAT 
ATTACATTTC TACATGAAGG AAATGATTCC AAAGTGGATG GCCTGGTAAA TTTTGAGAAA 
CTCAGAATGA TTGCCAAAGA AATCCGGCAA GTTATCAGAA TGACCTCTGC TAACATGGAT 
CCGGCTGTAA TGTTCAGGCA AAGGAAGAAG AGGTGGAGGA GTTTGGGGTC TCTGAGCCAG 
GGCAGCACAA ACTCAAATAT GCTCGATGTT CAAGGAGGTG CTCACAAAAA ACGTGCCCGA 
CGCAGTTCGC TCCTTAATGC CAAGAAGCTA TATGAGGATG CCCAGATGGC CAGAAAGGTG 
AAGCAGTACC TGTCCAACCT GAATGTAGAA ACTGATGAGG AAAAATTCCA GATCATGTCA 
CTTCAGTGCG AGCCTGCTTA TAGCACTTTG ACAAAGAATT TAAGTGAGAG AAGAGGTGGG 
AAATCCTCAG AAATGTCTCC AATACCCTTG AGGTCAGTTG GCCAAACCAC AAAAGTTCAG 
CAGCATCAGC AAAACAGAAT GAGCCAAGTT CTTCAAGTTC CTGCTGTTAA TTTGTATCCC 
ATTAGAAAAA AGGGACCAGC AAAGGACCAC GTCACTTTTA ATACAGGTTC TCCTCAGAAG 
TCATTAAGTT TGTCGGAGGA AGTAAGTAAG AAACCAGCAG AAGATGCTGT GTCTATGGCA 
TCTTCTTTGC ACTCCAGTCC TCTTGCATCT CCTCAGGGCT CTCCACGCAA AGTTGGTAAC 
ATCCAGAGGT CTGATAATTG CAGTCAGATG AATCTTTCTG GATCCTCTTC ATCGCTTGCC 
AGTGAAACCA GTAACAAGAA CAGTGGGCAA CGCAGCTATG GAATAGGCTA TGCTCTCATA 
CCTTCTGCCA AGTCAGATAA TTTCTCAGAC TCCAGCCACA GCGAGATCTC CTCACGGTCC 
AGCATAGTGA GCAACTGCTC TGTCGACTCC ATGTCTGCAG CCCTCCAGGA CGAGAGGGGT 
TTGCCCCAGT CTCTTAGTGT CGTGGATTCA GTGGGAACAG AAAGAAAAGA ACATCCTCCA 
CCATTGGCTG AGTACAGTCA GCCTTGCCCA GGTTGGCCCC TCCCTAGACC GGCTATAATC 
AGAGGATTGG CTGTCACATC GTCCATGAGC AATGAGGAGA TCTCCCATGA ACATATTACA 
ATAGAAGCCG CAGACAGTGG CCGTGGAAGC TGGACTTCAT GTTCAAGTAG TTCTCACGAT 
AACTTCCAGA ATATCCAAAT TCAGAAAAGC TGGGATCTCT TGAATTCTTA CCGCCACACT 
CACTTGGATG GACCTATTGC TGAAGTAGAA CCCACAAACT GTTACCCTGA AGAGGCGTAT 
TTGTATTCCG AAGCCTTTTC CAAAAATAAT AGGCAGTCCA AAACCAGCGT GGAATTGGAT 
CAGTCTCGGC AGAGCTGGGC CTCCTCTAGC TCTCTGTCAG ACACGTATGA AACAAACTAT 
GGGACGATTA AACGGAGAGG GCTGGAGAAC TCAACAATGG AGCAGACTGA GATTTTGGAC 
TCTAAACCAG GCACTGATGC AACTTACAAA ACTGTTACTT CTAGTACAGA AAAGGGCCTA 
ATAGTGTACT GTGTCACCTC ACCTAAGAAG GACGATAGGT ATAGGGAGCC ACCGCCCACC 
CCTCCGGGAT ATATGGGGAT TTCTTTAGCG GACATAAAGG AAGGATCGCC CCACCACCCA 
CACTTAAAAC CTCCAGACTA TAGTGTGGCA GTGCAGAGGT CAAAGATGCT ACATAGTGAC 
CTCTCTAGAC TTTCATCAGC TTCTCTCAGT AGTGAACCAG TGGCCTGTAT TTCATCGAAG 
ATGCCTCAGT GGCATACCCG GCAGCCGTCC CATCCTAAAC TAACAGATAT GACTGACGCA 
GATAGTGAAG AGGATGAAGA TGAACAAGTA TCAGCAGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019333913.1
CDS	430..4971
Translation	MAFLVRCYANCLQPWASKLPADLTKMQIADNPHPQVTHVPSSQSGCSIASDSGSSSLSDIYQATESEMGDVDLTGLPEAPVDSEDEEEEEDEDIDRTSDPLLGRDVVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISYPDGKTENLCMGNSFGITPSLEKQYMNGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVKEHRELDRSGTRKGHIVIKATPERLITHLIEEHSIVDPTYIEDFLLTYRTFLSSPLEVGDKLLEWFKIDSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFEKNLENTKMKGHLRLLNIACAAKAKWRQITLQKASRDSPLYFSLLGGSEKGFGIFVETVETGSKAAEVGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLSITVKTNIFVFKELLSRIGQEKNGVPHIPKIAEKKNNRYSIPDMPGDMEQIFPREKGNKKMKANTVSGGRNRIKKILDKTRFSILPPKLFSDGSVSQSQDDSIVGTRQCRHSLAIMPVPGTLSSSSPDLLQPTTSILDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVSHAVHEFNLTGASETYSLCEVSVSPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTIEVATQLSMRDFELFRNIEPTEYIDDLFKLDSKTGNAHLKQFEDVINQETFWVATEILLEPNQLKRMKIIKHFIKIALHCRECKNFNSMFAVISGLNLASVARLRGTWEKLPSKYEKHFRDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDITFLHEGNDSKVDGLVNFEKLRMIAKEIRQVIRMTSANMDPAVMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSNLNVETDEEKFQIMSLQCEPAYSTLTKNLSERRGGKSSEMSPIPLRSVGQTTKVQQHQQNRMSQVLQVPAVNLYPIRKKGPAKDHVTFNTGSPQKSLSLSEEVSKKPAEDAVSMASSLHSSPLASPQGSPRKVGNIQRSDNCSQMNLSGSSSSLASETSNKNSGQRSYGIGYALIPSAKSDNFSDSSHSEISSRSSIVSNCSVDSMSAALQDERGLPQSLSVVDSVGTERKEHPPPLAEYSQPCPGWPLPRPAIIRGLAVTSSMSNEEISHEHITIEAADSGRGSWTSCSSSSHDNFQNIQIQKSWDLLNSYRHTHLDGPIAEVEPTNCYPEEAYLYSEAFSKNNRQSKTSVELDQSRQSWASSSSLSDTYETNYGTIKRRGLENSTMEQTEILDSKPGTDATYKTVTSSTEKGLIVYCVTSPKKDDRYREPPPTPPGYMGISLADIKEGSPHHPHLKPPDYSVAVQRSKMLHSDLSRLSSASLSSEPVACISSKMPQWHTRQPSHPKLTDMTDADSEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_019478368.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019478368.1

ORF Insert Sequence:

ATGGCTTTTC TTGTTCGCTG TTACGCCAAC TGTCTGCAGC CATGGGCCTC CAAACTTCCA 
GCAGATCTTA CCAAGATGCA AATTGCAGAC AATCCACATC CACAGGTGAC TCATGTCCCT 
TCCAGCCAGT CTGGATGTAG CATTGCCAGT GACTCTGGGA GCAGCAGCTT GTCTGATATA 
TATCAGGCTA CAGAAAGTGA GATGGGAGAT GTAGATTTAA CAGGCCTCCC AGAAGCACCT 
GTGGACTCTG AAGATGAAGA GGAGGAGGAA GATGAAGATA TAGACAGAAC TTCAGATCCA 
TTATTGGGGC GAGACGTTGT ACGAGAGTGC CTTGAAAAAG AACCTGCAGA TAAAACGGAT 
GATGACATTG AGCAATTACT GGAATTTATG CATCAGCTCC CAGCTTTTGC AAACATGACC 
ATGTCTGTGA GAAGAGAGCT TTGTTCAGTT ATGATATTTG AAGTGGTAGA ACAAGCAGGA 
GCCATCATAC TTGAAGACGG ACAAGAACTT GATTCTTGGT ATGTTATTTT AAATGGCACT 
GTGGAGATCA GCTATCCAGA TGGGAAAACT GAAAATCTCT GTATGGGAAA TAGTTTTGGG 
ATTACTCCTT CTCTGGAAAA ACAGTATATG AATGGAGTGG TTAGAACCAA AGTAGATGAC 
TGTCAGTTTG TATGCATAGC CCAGCAAGAT TATTGGCGCA TTCTGAACCA CGTGGAAAAA 
AACACTCATA AAGTTGAGGA AGAAGGAGAA ATTGTTATGG TAAAGGAGCA CAGAGAGCTG 
GATCGCAGTG GAACCAGGAA AGGACACATA GTTATTAAGG CAACACCTGA GCGACTCATC 
ACACATTTAA TAGAAGAGCA TTCCATCGTT GATCCAACAT ACATAGAAGA CTTCCTTTTA 
ACATATAGAA CATTTCTCTC GAGCCCCTTG GAAGTTGGGG ATAAGCTCTT GGAGTGGTTT 
AAAATAGATA GCCTCAGGGA TAAGGTTACC AGAGTGGTCT TATTGTGGGT AAACAATCAT 
TTTAATGATT TTGAAGGAGA TCCTGCTATG ACACGTTTTC TGGAAGAATT TGAAAAAAAC 
TTGGAAAATA CGAAAATGAA GGGGCACCTC AGGTTGTTGA ATATTGCTTG TGCTGCCAAG 
GCAAAATGGA GGCAGATTAC CCTGCAGAAG GCTTCCAGAG ACTCGCCTCT GTATTTCAGC 
CTCCTGGGAG GAAGTGAGAA GGGTTTTGGT ATATTTGTAG AGACTGTAGA AACAGGCAGC 
AAAGCAGCAG AAGTTGGGCT GAAGCGTGGC GATCAGATAA TGGAAGTAAA TGGGCAGAAC 
TTTGAGAACA TTACTTTTGT GAAAGCCCTT GAAATCTTGA GGAACAATAC TCATCTTTCC 
ATTACAGTAA AGACAAACAT TTTTGTGTTT AAGGAACTGC TTAGCCGGAT AGGCCAGGAG 
AAAAACGGAG TCCCACATAT TCCAAAAATT GCAGAAAAGA AAAATAACCG TTACTCCATC 
CCAGATATGC CAGGAGACAT GGAACAGATA TTTCCACGTG AGAAAGGAAA CAAGAAAATG 
AAAGCAAACA CTGTATCCGG TGGAAGAAAT AGGATCAAGA AAATTTTAGA CAAGACTCGT 
TTCAGCATTT TGCCCCCAAA ACTATTTAGT GATGGCAGTG TAAGTCAATC CCAGGATGAC 
AGCATTGTTG GAACAAGGCA GTGCAGACAC AGCCTGGCCA TCATGCCTGT CCCTGGAACA 
CTTTCATCCA GCAGCCCGGA CCTTTTGCAG CCTACAACCA GCATTCTTGA TTTTTCTAAT 
CCTTCAGCGG TCGGTTTTTA CTATATTCCA GATCAAGTTA TTAGAGTTTT CAAAGCAGAT 
CAACAGAGTT GTTACATTAT CATCAGCAAA GACACTACAG CGAAAGAAGT TGTGAGTCAT 
GCTGTGCATG AATTCAACTT GACGGGTGCG TCAGAGACCT ACTCTCTCTG CGAGGTGTCT 
GTTAGTCCTG AGGGAGTCAT CAAACAAAGA AGGCTTCCAG ATCAGTTCTC AAAATTAGCA 
GACAGGATTC AGCTCAATGG AAGGTATTAT CTGAAAAACA ATATGGAAAC AGAGACCTTA 
TGCTCAGATG AAGATGCCCA AGAGCTGCTC AGAGAGAGTC AAATATCTCT TCTTCAGCTG 
AGCACCATTG AAGTGGCAAC TCAGCTTTCA ATGAGAGACT TTGAATTATT TCGCAACATT 
GAGCCTACTG AATACATTGA TGACCTTTTT AAGCTGGACT CAAAAACAGG AAATGCTCAC 
CTGAAACAAT TTGAGGATGT CATTAACCAA GAGACATTTT GGGTTGCCAC AGAAATTTTA 
TTAGAGCCCA ACCAGCTCAA AAGAATGAAA ATTATTAAAC ATTTCATTAA AATTGCCCTC 
CACTGTCGAG AATGCAAGAA CTTCAATTCC ATGTTTGCAG TTATAAGTGG GCTAAATCTG 
GCATCGGTAG CACGACTCAG AGGTACCTGG GAAAAATTAC CTAGCAAGTA TGAAAAGCAT 
TTCAGAGACC TTCAGGATCT TTTTGATCCA TCTAGAAACA TGGCAAAATA CCGCAATATT 
CTTAGCAGTC AGAGCATGCA GCCTCCAATT ATTCCACTCT TTCCCGTTGT CAAGAAGGAT 
ATTACATTTC TACATGAAGG AAATGATTCC AAAGTGGATG GCCTGGTAAA TTTTGAGAAA 
CTCAGAATGA TTGCCAAAGA AATCCGGCAA GTTATCAGAA TGACCTCTGC TAACATGGAT 
CCGGCTGTAA TGTTCAGGCA AAGGAAGAAG AGGTGGAGGA GTTTGGGGTC TCTGAGCCAG 
GGCAGCACAA ACTCAAATAT GCTCGATGTT CAAGGAGGTG CTCACAAAAA ACGTGCCCGA 
CGCAGTTCGC TCCTTAATGC CAAGAAGCTA TATGAGGATG CCCAGATGGC CAGAAAGGTG 
AAGCAGTACC TGTCCAACCT GAATGTAGAA ACTGATGAGG AAAAATTCCA GATCATGTCA 
CTTCAGTGCG AGCCTGCTTA TAGCACTTTG ACAAAGAATT TAAGTGAGAG AAGAGGTGGG 
AAATCCTCAG AAATGTCTCC AATACCCTTG AGGTCAGTTG GCCAAACCAC AAAAGTTCAG 
CAGCATCAGC AAAACAGAAT GAGCCAAGTT CTTCAAGTTC CTGCTGTTAA TTTGTATCCC 
ATTAGAAAAA AGGGACCAGC AAAGGACCAC GTCACTTTTA ATACAGGTTC TCCTCAGAAG 
TCATTAAGTT TGTCGGAGGA AGTAAGTAAG AAACCAGCAG AAGATGCTGT GTCTATGGCA 
TCTTCTTTGC ACTCCAGTCC TCTTGCATCT CCTCAGGGCT CTCCACGCAA AGTTGGTAAC 
ATCCAGAGGT CTGATAATTG CAGTCAGATG AATCTTTCTG GATCCTCTTC ATCGCTTGCC 
AGTGAAACCA GTAACAAGAA CAGTGGGCAA CGCAGCTATG GAATAGGCTA TGCTCTCATA 
CCTTCTGCCA AGTCAGATAA TTTCTCAGAC TCCAGCCACA GCGAGATCTC CTCACGGTCC 
AGCATAGTGA GCAACTGCTC TGTCGACTCC ATGTCTGCAG CCCTCCAGGA CGAGAGGGGT 
TTGCCCCAGT CTCTTAGTGT CGTGGATTCA GTGGGAACAG AAAGAAAAGA ACATCCTCCA 
CCATTGGCTG AGTACAGTCA GCCTTGCCCA GGTTGGCCCC TCCCTAGACC GGCTATAATC 
AGAGGATTGG CTGTCACATC GTCCATGAGC AATGAGGAGA TCTCCCATGA ACATATTACA 
ATAGAAGCCG CAGACAGTGG CCGTGGAAGC TGGACTTCAT GTTCAAGTAG TTCTCACGAT 
AACTTCCAGA ATATCCAAAT TCAGAAAAGC TGGGATCTCT TGAATTCTTA CCGCCACACT 
CACTTGGATG GACCTATTGC TGAAGTAGAA CCCACAAACT GTTACCCTGA AGAGGCGTAT 
TTGTATTCCG AAGCCTTTTC CAAAAATAAT AGGCAGTCCA AAACCAGCGT GGAATTGGAT 
CAGTCTCGGC AGAGCTGGGC CTCCTCTAGC TCTCTGTCAG ACACGTATGA AACAAACTAT 
GGGACGATTA AACGGAGAGG GCTGGAGAAC TCAACAATGG AGCAGACTGA GATTTTGGAC 
TCTAAACCAG GCACTGATGC AACTTACAAA ACTGTTACTT CTAGTACAGA AAAGGGCCTA 
ATAGTGTACT GTGTCACCTC ACCTAAGAAG GACGATAGGT ATAGGGAGCC ACCGCCCACC 
CCTCCGGGAT ATATGGGGAT TTCTTTAGCG GACATAAAGG AAGGATCGCC CCACCACCCA 
CACTTAAAAC CTCCAGACTA TAGTGTGGCA GTGCAGAGGT CAAAGATGCT ACATAGTGAC 
CTCTCTAGAC TTTCATCAGC TTCTCTCAGT AGTGAACCAG TGGCCTGTAT TTCATCGAAG 
ATGCCTCAGT GGCATACCCG GCAGCCGTCC CATCCTAAAC TAACAGATAT GACTGACGCA 
GATAGTGAAG AGGATGAAGA TGAACAAGTA TCAGCAGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_019478369.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019333914.1 Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OAl76332
Clone ID Related Accession (Same CDS sequence)	XM_019478369.1
Accession Version	XM_019478369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	rap guanine nucleotide exchange factor 6 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017713851.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCCG CCGTGGACCC CAAGGTCCAA CAGGCCCTGC GGAAGAAGCC CCCGGAGCGG 
AGCCGCGAGG ACTTAAATAT TATCTATACT TATCTTCATG GAATGGACAT ATTGTCTCAT 
CTCAGGGAAC ATCAGCTAAG AATAATGTCT TCAAGTGCCC GCTATGAAAG ATACAAGGGA 
AACCAAGTTC TCTTTTGCTC AGAAACAATC GCCAGATGTT GGTACATTCT TCTTTCCGGA 
TCTGTGCTTA TGAAGGACTC CATGTTTTTA CCACCATGCA GTTTTGGCAA GCAGTCTGGA 
GGAAAGCGAG GATGCGAGTG TATTGTACTG GAGCCTTCTG AAATGATTGT GGTGGAGAAC 
TCCAAAGACA ACGAAGAAAA TATCTTGCAA AGAGAAGTTC CCATCAGACA GTCTCGGCGA 
AGGTTCAGGA AGATTAATCA ACGGGGAGAA CGCCAGACCA TCACTGACCA TGTGGATGCT 
AGCAGTTATC TTTCGCTTCC AGCAGATCTT ACCAAGATGC AAATTGCAGA CAATCCACAT 
CCACAGGTGA CTCATGTCCC TTCCAGCCAG TCTGGATGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGCT TGTCTGATAT ATATCAGGCT ACAGAAAGTG AGATGGGAGA TGTAGATTTA 
ACAGGCCTCC CAGAAGCACC TGTGGACTCT GAAGATGAAG AGGAGGAGGA AGATGAAGAT 
ATAGACAGAA CTTCAGATCC ATTATTGGGG CGAGACGTTG TACGAGAGTG CCTTGAAAAA 
GAACCTGCAG ATAAAACGGA TGATGACATT GAGCAATTAC TGGAATTTAT GCATCAGCTC 
CCAGCTTTTG CAAACATGAC CATGTCTGTG AGAAGAGAGC TTTGTTCAGT TATGATATTT 
GAAGTGGTAG AACAAGCAGG AGCCATCATA CTTGAAGACG GACAAGAACT TGATTCTTGG 
TATGTTATTT TAAATGGCAC TGTGGAGATC AGCTATCCAG ATGGGAAAAC TGAAAATCTC 
TGTATGGGAA ATAGTTTTGG GATTACTCCT TCTCTGGAAA AACAGTATAT GAATGGAGTG 
GTTAGAACCA AAGTAGATGA CTGTCAGTTT GTATGCATAG CCCAGCAAGA TTATTGGCGC 
ATTCTGAACC ACGTGGAAAA AAACACTCAT AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAGGAGC ACAGAGAGCT GGATCGCAGT GGAACCAGGA AAGGACACAT AGTTATTAAG 
GCAACACCTG AGCGACTCAT CACACATTTA ATAGAAGAGC ATTCCATCGT TGATCCAACA 
TACATAGAAG ACTTCCTTTT AACATATAGA ACATTTCTCT CGAGCCCCTT GGAAGTTGGG 
GATAAGCTCT TGGAGTGGTT TAAAATAGAT AGCCTCAGGG ATAAGGTTAC CAGAGTGGTC 
TTATTGTGGG TAAACAATCA TTTTAATGAT TTTGAAGGAG ATCCTGCTAT GACACGTTTT 
CTGGAAGAAT TTGAAAAAAA CTTGGAAAAT ACGAAAATGA AGGGGCACCT CAGGTTGTTG 
AATATTGCTT GTGCTGCCAA GGCAAAATGG AGGCAGATTA CCCTGCAGAA GGCTTCCAGA 
GACTCGCCTC TGTATTTCAG CCTCCTGGGA GGAAGTGAGA AGGGTTTTGG TATATTTGTA 
GAGACTGTAG AAACAGGCAG CAAAGCAGCA GAAGTTGGGC TGAAGCGTGG CGATCAGATA 
ATGGAAGTAA ATGGGCAGAA CTTTGAGAAC ATTACTTTTG TGAAAGCCCT TGAAATCTTG 
AGGAACAATA CTCATCTTTC CATTACAGTA AAGACAAACA TTTTTGTGTT TAAGGAACTG 
CTTAGCCGGA TAGGCCAGGA GAAAAACGGA GTCCCACATA TTCCAAAAAT TGCAGAAAAG 
AAAAATAACC GTTACTCCAT CCCAGATATG CCAGGAGACA TGGAACAGAT ATTTCCACGT 
GAGAAAGGAA ACAAGAAAAT GAAAGCAAAC ACTGTATCCG GTGGAAGAAA TAGGATCAAG 
AAAATTTTAG ACAAGACTCG TTTCAGCATT TTGCCCCCAA AACTATTTAG TGATGGCAGT 
GTAAGTCAAT CCCAGGATGA CAGCATTGTT GGAACAAGGC AGTGCAGACA CAGCCTGGCC 
ATCATGCCTG TCCCTGGAAC ACTTTCATCC AGCAGCCCGG ACCTTTTGCA GCCTACAACC 
AGCATTCTTG ATTTTTCTAA TCCTTCAGAT ATTCCAGATC AAGTTATTAG AGTTTTCAAA 
GCAGATCAAC AGAGTTGTTA CATTATCATC AGCAAAGACA CTACAGCGAA AGAAGTTGTG 
AGTCATGCTG TGCATGAATT CAACTTGACG GGTGCGTCAG AGACCTACTC TCTCTGCGAG 
GTGTCTGTTA GTCCTGAGGG AGTCATCAAA CAAAGAAGGC TTCCAGATCA GTTCTCAAAA 
TTAGCAGACA GGATTCAGCT CAATGGAAGG TATTATCTGA AAAACAATAT GGAAACAGAG 
ACCTTATGCT CAGATGAAGA TGCCCAAGAG CTGCTCAGAG AGAGTCAAAT ATCTCTTCTT 
CAGCTGAGCA CCATTGAAGT GGCAACTCAG CTTTCAATGA GAGACTTTGA ATTATTTCGC 
AACATTGAGC CTACTGAATA CATTGATGAC CTTTTTAAGC TGGACTCAAA AACAGGAAAT 
GCTCACCTGA AACAATTTGA GGATGTCATT AACCAAGAGA CATTTTGGGT TGCCACAGAA 
ATTTTATTAG AGCCCAACCA GCTCAAAAGA ATGAAAATTA TTAAACATTT CATTAAAATT 
GCCCTCCACT GTCGAGAATG CAAGAACTTC AATTCCATGT TTGCAGTTAT AAGTGGGCTA 
AATCTGGCAT CGGTAGCACG ACTCAGAGGT ACCTGGGAAA AATTACCTAG CAAGTATGAA 
AAGCATTTCA GAGACCTTCA GGATCTTTTT GATCCATCTA GAAACATGGC AAAATACCGC 
AATATTCTTA GCAGTCAGAG CATGCAGCCT CCAATTATTC CACTCTTTCC CGTTGTCAAG 
AAGGATATTA CATTTCTACA TGAAGGAAAT GATTCCAAAG TGGATGGCCT GGTAAATTTT 
GAGAAACTCA GAATGATTGC CAAAGAAATC CGGCAAGTTA TCAGAATGAC CTCTGCTAAC 
ATGGATCCGG CTGTAATGTT CAGGCAAAGG AAGAAGAGGT GGAGGAGTTT GGGGTCTCTG 
AGCCAGGGCA GCACAAACTC AAATATGCTC GATGTTCAAG GAGGTGCTCA CAAAAAACGT 
GCCCGACGCA GTTCGCTCCT TAATGCCAAG AAGCTATATG AGGATGCCCA GATGGCCAGA 
AAGGTGAAGC AGTACCTGTC CAACCTGAAT GTAGAAACTG ATGAGGAAAA ATTCCAGATC 
ATGTCACTTC AGTGCGAGCC TGCTTATAGC ACTTTGACAA AGAATTTAAG TGAGAGAAGA 
GGTGGGAAAT CCTCAGAAAT GTCTCCAATA CCCTTGAGGT CAGTTGGCCA AACCACAAAA 
GTTCAGCAGC ATCAGCAAAA CAGAATGAGC CAAGTTCTTC AAGTTCCTGC TGTTAATTTG 
TATCCCATTA GAAAAAAGGG ACCAGCAAAG GACCACGTCA CTTTTAATAC AGGTTCTCCT 
CAGAAGTCAT TAAGTTTGTC GGAGGAAGTA AGTAAGAAAC CAGCAGAAGA TGCTGTGTCT 
ATGGCATCTT CTTTGCACTC CAGTCCTCTT GCATCTCCTC AGGGCTCTCC ACGCAAAGTT 
GGTAACATCC AGAGGTCTGA TAATTGCAGT CAGATGAATC TTTCTGGATC CTCTTCATCG 
CTTGCCAGTG AAACCAGTAA CAAGAACAGT GGGCAACGCA GCTATGGAAT AGGCTATGCT 
CTCATACCTT CTGCCAAGTC AGATAATTTC TCAGACTCCA GCCACAGCGA GATCTCCTCA 
CGGTCCAGCA TAGTGAGCAA CTGCTCTGTC GACTCCATGT CTGCAGCCCT CCAGGACGAG 
AGGGGTTTGC CCCAGTCTCT TAGTGTCGTG GATTCAGTGG GAACAGAAAG AAAAGAACAT 
CCTCCACCAT TGGCTGAGTA CAGTCAGCCT TGCCCAGGTT GGCCCCTCCC TAGACCGGCT 
ATAATCAGAG GATTGGCTGT CACATCGTCC ATGAGCAATG AGGAGATCTC CCATGAACAT 
ATTACAATAG AAGCCGCAGA CAGTGGCCGT GGAAGCTGGA CTTCATGTTC AAGTAGTTCT 
CACGATAACT TCCAGAATAT CCAAATTCAG AAAAGCTGGG ATCTCTTGAA TTCTTACCGC 
CACACTCACT TGGATGGACC TATTGCTGAA GTAGAACCCA CAAACTGTTA CCCTGAAGAG 
GCGTATTTGT ATTCCGAAGC CTTTTCCAAA AATAATAGGC AGTCCAAAAC CAGCGTGGAA 
TTGGATCAGT CTCGGCAGAG CTGGGCCTCC TCTAGCTCTC TGTCAGACAC GTATGAAACA 
AACTATGGGA CGATTAAACG GAGAGGGCTG GAGAACTCAA CAATGGAGCA GACTGAGATT 
TTGGACTCTA AACCAGGCAC TGATGCAACT TACAAAACTG TTACTTCTAG TACAGAAAAG 
GGCCTAATAG AAGATGAACA AGTATCAGCA GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019333914.1
CDS	270..4925
Translation	MGSAVDPKVQQALRKKPPERSREDLNIIYTYLHGMDILSHLREHQLRIMSSSARYERYKGNQVLFCSETIARCWYILLSGSVLMKDSMFLPPCSFGKQSGGKRGCECIVLEPSEMIVVENSKDNEENILQREVPIRQSRRRFRKINQRGERQTITDHVDASSYLSLPADLTKMQIADNPHPQVTHVPSSQSGCSIASDSGSSSLSDIYQATESEMGDVDLTGLPEAPVDSEDEEEEEDEDIDRTSDPLLGRDVVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISYPDGKTENLCMGNSFGITPSLEKQYMNGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVKEHRELDRSGTRKGHIVIKATPERLITHLIEEHSIVDPTYIEDFLLTYRTFLSSPLEVGDKLLEWFKIDSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFEKNLENTKMKGHLRLLNIACAAKAKWRQITLQKASRDSPLYFSLLGGSEKGFGIFVETVETGSKAAEVGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLSITVKTNIFVFKELLSRIGQEKNGVPHIPKIAEKKNNRYSIPDMPGDMEQIFPREKGNKKMKANTVSGGRNRIKKILDKTRFSILPPKLFSDGSVSQSQDDSIVGTRQCRHSLAIMPVPGTLSSSSPDLLQPTTSILDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVSHAVHEFNLTGASETYSLCEVSVSPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTIEVATQLSMRDFELFRNIEPTEYIDDLFKLDSKTGNAHLKQFEDVINQETFWVATEILLEPNQLKRMKIIKHFIKIALHCRECKNFNSMFAVISGLNLASVARLRGTWEKLPSKYEKHFRDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDITFLHEGNDSKVDGLVNFEKLRMIAKEIRQVIRMTSANMDPAVMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSNLNVETDEEKFQIMSLQCEPAYSTLTKNLSERRGGKSSEMSPIPLRSVGQTTKVQQHQQNRMSQVLQVPAVNLYPIRKKGPAKDHVTFNTGSPQKSLSLSEEVSKKPAEDAVSMASSLHSSPLASPQGSPRKVGNIQRSDNCSQMNLSGSSSSLASETSNKNSGQRSYGIGYALIPSAKSDNFSDSSHSEISSRSSIVSNCSVDSMSAALQDERGLPQSLSVVDSVGTERKEHPPPLAEYSQPCPGWPLPRPAIIRGLAVTSSMSNEEISHEHITIEAADSGRGSWTSCSSSSHDNFQNIQIQKSWDLLNSYRHTHLDGPIAEVEPTNCYPEEAYLYSEAFSKNNRQSKTSVELDQSRQSWASSSSLSDTYETNYGTIKRRGLENSTMEQTEILDSKPGTDATYKTVTSSTEKGLIEDEQVSAV

Target ORF information:

RefSeq Version	XM_019478369.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019478369.1

ORF Insert Sequence:

ATGGGCTCCG CCGTGGACCC CAAGGTCCAA CAGGCCCTGC GGAAGAAGCC CCCGGAGCGG 
AGCCGCGAGG ACTTAAATAT TATCTATACT TATCTTCATG GAATGGACAT ATTGTCTCAT 
CTCAGGGAAC ATCAGCTAAG AATAATGTCT TCAAGTGCCC GCTATGAAAG ATACAAGGGA 
AACCAAGTTC TCTTTTGCTC AGAAACAATC GCCAGATGTT GGTACATTCT TCTTTCCGGA 
TCTGTGCTTA TGAAGGACTC CATGTTTTTA CCACCATGCA GTTTTGGCAA GCAGTCTGGA 
GGAAAGCGAG GATGCGAGTG TATTGTACTG GAGCCTTCTG AAATGATTGT GGTGGAGAAC 
TCCAAAGACA ACGAAGAAAA TATCTTGCAA AGAGAAGTTC CCATCAGACA GTCTCGGCGA 
AGGTTCAGGA AGATTAATCA ACGGGGAGAA CGCCAGACCA TCACTGACCA TGTGGATGCT 
AGCAGTTATC TTTCGCTTCC AGCAGATCTT ACCAAGATGC AAATTGCAGA CAATCCACAT 
CCACAGGTGA CTCATGTCCC TTCCAGCCAG TCTGGATGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGCT TGTCTGATAT ATATCAGGCT ACAGAAAGTG AGATGGGAGA TGTAGATTTA 
ACAGGCCTCC CAGAAGCACC TGTGGACTCT GAAGATGAAG AGGAGGAGGA AGATGAAGAT 
ATAGACAGAA CTTCAGATCC ATTATTGGGG CGAGACGTTG TACGAGAGTG CCTTGAAAAA 
GAACCTGCAG ATAAAACGGA TGATGACATT GAGCAATTAC TGGAATTTAT GCATCAGCTC 
CCAGCTTTTG CAAACATGAC CATGTCTGTG AGAAGAGAGC TTTGTTCAGT TATGATATTT 
GAAGTGGTAG AACAAGCAGG AGCCATCATA CTTGAAGACG GACAAGAACT TGATTCTTGG 
TATGTTATTT TAAATGGCAC TGTGGAGATC AGCTATCCAG ATGGGAAAAC TGAAAATCTC 
TGTATGGGAA ATAGTTTTGG GATTACTCCT TCTCTGGAAA AACAGTATAT GAATGGAGTG 
GTTAGAACCA AAGTAGATGA CTGTCAGTTT GTATGCATAG CCCAGCAAGA TTATTGGCGC 
ATTCTGAACC ACGTGGAAAA AAACACTCAT AAAGTTGAGG AAGAAGGAGA AATTGTTATG 
GTAAAGGAGC ACAGAGAGCT GGATCGCAGT GGAACCAGGA AAGGACACAT AGTTATTAAG 
GCAACACCTG AGCGACTCAT CACACATTTA ATAGAAGAGC ATTCCATCGT TGATCCAACA 
TACATAGAAG ACTTCCTTTT AACATATAGA ACATTTCTCT CGAGCCCCTT GGAAGTTGGG 
GATAAGCTCT TGGAGTGGTT TAAAATAGAT AGCCTCAGGG ATAAGGTTAC CAGAGTGGTC 
TTATTGTGGG TAAACAATCA TTTTAATGAT TTTGAAGGAG ATCCTGCTAT GACACGTTTT 
CTGGAAGAAT TTGAAAAAAA CTTGGAAAAT ACGAAAATGA AGGGGCACCT CAGGTTGTTG 
AATATTGCTT GTGCTGCCAA GGCAAAATGG AGGCAGATTA CCCTGCAGAA GGCTTCCAGA 
GACTCGCCTC TGTATTTCAG CCTCCTGGGA GGAAGTGAGA AGGGTTTTGG TATATTTGTA 
GAGACTGTAG AAACAGGCAG CAAAGCAGCA GAAGTTGGGC TGAAGCGTGG CGATCAGATA 
ATGGAAGTAA ATGGGCAGAA CTTTGAGAAC ATTACTTTTG TGAAAGCCCT TGAAATCTTG 
AGGAACAATA CTCATCTTTC CATTACAGTA AAGACAAACA TTTTTGTGTT TAAGGAACTG 
CTTAGCCGGA TAGGCCAGGA GAAAAACGGA GTCCCACATA TTCCAAAAAT TGCAGAAAAG 
AAAAATAACC GTTACTCCAT CCCAGATATG CCAGGAGACA TGGAACAGAT ATTTCCACGT 
GAGAAAGGAA ACAAGAAAAT GAAAGCAAAC ACTGTATCCG GTGGAAGAAA TAGGATCAAG 
AAAATTTTAG ACAAGACTCG TTTCAGCATT TTGCCCCCAA AACTATTTAG TGATGGCAGT 
GTAAGTCAAT CCCAGGATGA CAGCATTGTT GGAACAAGGC AGTGCAGACA CAGCCTGGCC 
ATCATGCCTG TCCCTGGAAC ACTTTCATCC AGCAGCCCGG ACCTTTTGCA GCCTACAACC 
AGCATTCTTG ATTTTTCTAA TCCTTCAGAT ATTCCAGATC AAGTTATTAG AGTTTTCAAA 
GCAGATCAAC AGAGTTGTTA CATTATCATC AGCAAAGACA CTACAGCGAA AGAAGTTGTG 
AGTCATGCTG TGCATGAATT CAACTTGACG GGTGCGTCAG AGACCTACTC TCTCTGCGAG 
GTGTCTGTTA GTCCTGAGGG AGTCATCAAA CAAAGAAGGC TTCCAGATCA GTTCTCAAAA 
TTAGCAGACA GGATTCAGCT CAATGGAAGG TATTATCTGA AAAACAATAT GGAAACAGAG 
ACCTTATGCT CAGATGAAGA TGCCCAAGAG CTGCTCAGAG AGAGTCAAAT ATCTCTTCTT 
CAGCTGAGCA CCATTGAAGT GGCAACTCAG CTTTCAATGA GAGACTTTGA ATTATTTCGC 
AACATTGAGC CTACTGAATA CATTGATGAC CTTTTTAAGC TGGACTCAAA AACAGGAAAT 
GCTCACCTGA AACAATTTGA GGATGTCATT AACCAAGAGA CATTTTGGGT TGCCACAGAA 
ATTTTATTAG AGCCCAACCA GCTCAAAAGA ATGAAAATTA TTAAACATTT CATTAAAATT 
GCCCTCCACT GTCGAGAATG CAAGAACTTC AATTCCATGT TTGCAGTTAT AAGTGGGCTA 
AATCTGGCAT CGGTAGCACG ACTCAGAGGT ACCTGGGAAA AATTACCTAG CAAGTATGAA 
AAGCATTTCA GAGACCTTCA GGATCTTTTT GATCCATCTA GAAACATGGC AAAATACCGC 
AATATTCTTA GCAGTCAGAG CATGCAGCCT CCAATTATTC CACTCTTTCC CGTTGTCAAG 
AAGGATATTA CATTTCTACA TGAAGGAAAT GATTCCAAAG TGGATGGCCT GGTAAATTTT 
GAGAAACTCA GAATGATTGC CAAAGAAATC CGGCAAGTTA TCAGAATGAC CTCTGCTAAC 
ATGGATCCGG CTGTAATGTT CAGGCAAAGG AAGAAGAGGT GGAGGAGTTT GGGGTCTCTG 
AGCCAGGGCA GCACAAACTC AAATATGCTC GATGTTCAAG GAGGTGCTCA CAAAAAACGT 
GCCCGACGCA GTTCGCTCCT TAATGCCAAG AAGCTATATG AGGATGCCCA GATGGCCAGA 
AAGGTGAAGC AGTACCTGTC CAACCTGAAT GTAGAAACTG ATGAGGAAAA ATTCCAGATC 
ATGTCACTTC AGTGCGAGCC TGCTTATAGC ACTTTGACAA AGAATTTAAG TGAGAGAAGA 
GGTGGGAAAT CCTCAGAAAT GTCTCCAATA CCCTTGAGGT CAGTTGGCCA AACCACAAAA 
GTTCAGCAGC ATCAGCAAAA CAGAATGAGC CAAGTTCTTC AAGTTCCTGC TGTTAATTTG 
TATCCCATTA GAAAAAAGGG ACCAGCAAAG GACCACGTCA CTTTTAATAC AGGTTCTCCT 
CAGAAGTCAT TAAGTTTGTC GGAGGAAGTA AGTAAGAAAC CAGCAGAAGA TGCTGTGTCT 
ATGGCATCTT CTTTGCACTC CAGTCCTCTT GCATCTCCTC AGGGCTCTCC ACGCAAAGTT 
GGTAACATCC AGAGGTCTGA TAATTGCAGT CAGATGAATC TTTCTGGATC CTCTTCATCG 
CTTGCCAGTG AAACCAGTAA CAAGAACAGT GGGCAACGCA GCTATGGAAT AGGCTATGCT 
CTCATACCTT CTGCCAAGTC AGATAATTTC TCAGACTCCA GCCACAGCGA GATCTCCTCA 
CGGTCCAGCA TAGTGAGCAA CTGCTCTGTC GACTCCATGT CTGCAGCCCT CCAGGACGAG 
AGGGGTTTGC CCCAGTCTCT TAGTGTCGTG GATTCAGTGG GAACAGAAAG AAAAGAACAT 
CCTCCACCAT TGGCTGAGTA CAGTCAGCCT TGCCCAGGTT GGCCCCTCCC TAGACCGGCT 
ATAATCAGAG GATTGGCTGT CACATCGTCC ATGAGCAATG AGGAGATCTC CCATGAACAT 
ATTACAATAG AAGCCGCAGA CAGTGGCCGT GGAAGCTGGA CTTCATGTTC AAGTAGTTCT 
CACGATAACT TCCAGAATAT CCAAATTCAG AAAAGCTGGG ATCTCTTGAA TTCTTACCGC 
CACACTCACT TGGATGGACC TATTGCTGAA GTAGAACCCA CAAACTGTTA CCCTGAAGAG 
GCGTATTTGT ATTCCGAAGC CTTTTCCAAA AATAATAGGC AGTCCAAAAC CAGCGTGGAA 
TTGGATCAGT CTCGGCAGAG CTGGGCCTCC TCTAGCTCTC TGTCAGACAC GTATGAAACA 
AACTATGGGA CGATTAAACG GAGAGGGCTG GAGAACTCAA CAATGGAGCA GACTGAGATT 
TTGGACTCTA AACCAGGCAC TGATGCAACT TACAAAACTG TTACTTCTAG TACAGAAAAG 
GGCCTAATAG AAGATGAACA AGTATCAGCA GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

RAPGEF6 cDNA ORF clone, Alligator mississippiensis(American alligator)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム