ANKRD28 cDNA ORF clone, Acanthisitta chloris(rifleman)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKRD28
Entrez Gene ID	103797009
Full Name	ankyrin repeat domain 28
Gene Type	protein-coding
Organism	Acanthisitta chloris(rifleman)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009069199.1	XP_009067447.1	serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A isoform X1
XM_009069201.1	XP_009067449.1	serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A isoform X2

Pathways from BioSystems
Gene Ontology

The following ANKRD28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKRD28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc116502	XM_009069199.1 Latest version!	Acanthisitta chloris ankyrin repeat domain 28 (ANKRD28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAc116503	XM_009069201.1 Latest version!	Acanthisitta chloris ankyrin repeat domain 28 (ANKRD28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANKRD28 ( XM_009069199.1 ) cDNA ORF clone, Acanthisitta chloris(rifleman) -> XP_009067447.1 Acanthisitta chloris ankyrin repeat domain 28 (ANKRD28), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc116502
Clone ID Related Accession (Same CDS sequence)	XM_009069199.1
Accession Version	XM_009069199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-04
Organism	Acanthisitta chloris(rifleman)
Product	serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008658925.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthisitta chloris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAG TCTGTATTGT TGTTTTGGAG GAGGTGGAAG ATGATTCTCC CACATTTATT 
TCCGATTTAC CTCAGGAAAA TGTACCTTTA CATCCACCAC CCTCTGGAAA TGTGCTGGTA 
AGGCATCCAC CATTGGTTCA AGCTATATTT AATGGAGATC CTGATGAAGT TCGAGCACTA 
ATATTTAAGA AAGAAGATGT TAATTTTCAG GATAATGAGA AAAGGACCCC TTTACATGCT 
GCTGCCTATC TGGGAGATGC AGAAATTATT GAACTACTTA TTTTATCTGG AGCTAGAGTT 
AATGCCAAAG ACAGCAAATG GTTGACTCCT TTACACAGAG CCGTAGCATC TTGTAGTGAG 
GATGCTGTTC AGGTGTTGTT AAAGCATTCA GCAGATGTCA ATGCTCGTGA CAAAAACTGG 
CAGACACCCC TACATATAGC AGCTGCAAAC AAAGCTGTGA AATGTGCTGA AGCTTTGGTG 
CCTCTCCTAA GCAATGTAAA TGTGTCTGAT CGAGCAGGCA GAACTGCATT GCATCATGCA 
GCCTTCAGTG GACACGTTGA GATGGTCAGC TTACTGTTGT CTAGAGGGGC CAATATTAAT 
GCTTTTGACA AGAAGGACAG ACGAGCTATA CATTGGGCAG CATATATGGG TCACATTGAA 
GTTGTGAAAT TACTTGTGGC ACATACAGCT GAAGTGACAT GCAAAGACAA GAAATCATAT 
ACACCCTTAC ATGCTGCAGC ATCTAGTGGG ATGATCAGTG TAGTCAAGTA TCTTCTAGAT 
CTTGGAGTTG ATATGAACGA ACCAAATGCC TATGGAAATA CTCCTCTCCA TGTAGCTTGC 
TACAACGGTC AAGATGTTGT AGTGAATGAA CTCATAGACT GCGGTGCTAA TGTAAATCAA 
GTGAATGAGA AGGGGTTTAC ACCTTTGCAC TTTGCTGCTG CATCGACACA TGGAGCATTG 
TGTTTAGAGC TTTTGGTCTG CAACGGAGCA GATGTAAATA TAAAGAGTAA AGATGGGAAA 
ACACCTTTGC ACATGACAGC GATCCATGGT AGATTTTCAA GATCCCAAAC CATCATTCAA 
AATGGAGCTG AAATAGACTG TGAAGATAAG AATGGAAATA CTCCTTTGCA CATAGCAGCT 
AGATATGGCC ACGAGCTATT AATCAACACT TTGATTACAA GTGGTGCTGA CACTGCAAAG 
CGGGGTATAC ATGGGATGTT TCCTCTCCAT TTGGCAGCAT TAAGTGGATT TTCAGACTGC 
TGCAGAAAGC TCCTCTCATC AGGTTTTGAC ATTGACACAC CAGATGACTT TGGCAGGACT 
TGTCTTCACG CAGCTGCAGC TGGAGGGAAT TTGGAGTGCC TAAATCTTCT GCTAAATACT 
GGTGCAGACT TCAACAAAAA GGACAGATTT GGGAGAACTC CACTCCATTA TGCTGCTGCC 
AATTGTAATT ATCAGTGCCT GTTTGCCCTT GTGGGATCTG GAGCTAGTGT GAATGACCTT 
GATGAGAGGG GTTGTACACC TCTGCACTAT GCAGCTGCAT CAGACACAGA TGGGAAGTGC 
CTGGAATACT TGCTAAGAAA CGACGCCAAC CCGGGGATCC GAGACAAACA AGGATACAAT 
GCAGTTCATT ATTCAGCTGC TTATGGTCAT CGCTTATGCC TTGAATTGAT TGCAAGCGAA 
ACACCTCTGG ATGTTCTAAT GGAAACATCA GGTACTGACA TGCTGAATGA TTCAGACAAC 
AGAGCACCCA TAAGTCCGCT GCACTTAGCT GCCTATCATG GCCACCATCA AGCTCTGGAA 
GTGCTGGTGC AGTCACTGCT GGACCTTGAT GTGAGGAATA ACAATGGAAG GACACCACTG 
GATCTTGCTG CCTTTAAGGG ACACGTGGAA TGTGTAGATG TGCTCATTAA TCAGGGAGCA 
TCCATCCTGG TGAAAGATTA TGTTGTGAAG AGGACCCCAA TACATGCTGC AGCTACAAAT 
GGCCATTCAG AATGTTTGCG GCTATTGATA GGAAATGCAG AACCACAGAA TGCAGTGGAC 
ATTCAAGATG GAAATGGACA GACTCCTCTG ATGTTGTCAG TTCTCAACGG GCACACAGAC 
TGTGTTTATT CACTCCTCAA CAAAGGAGCA AATGTAGATG CCAAAGATAA GTGGGGAAGG 
ACAGCATTAC ACAGAGGGGC AGTTACTGGG CATGAGGAAT GTGTGGAAGC ATTGCTTCAA 
CATGGTGCTA AGTCCTTATT ACGAGACTGT AGGGGACGAA CCCCTATACA CCTGTCAGCT 
GCATGTGGCC ATATAGGTGT TCTGGGAGCT CTCCTGCAGT CAGCTACATC TGTGGATGCA 
GTGCCTGCAA TAGCAGACAA TCACGGCTAT ACATCACTGC ATTGGGCCTG CTATAATGGT 
CATGACAGTT GTGTAGAGCT GCTCCTGGAA CAGGAAGTTT TTCAGAAAAT GGAAGGAAAT 
TCTTTCAGTC CCTTGCATTG TGCTGTGATA AATGACAATG AAGGTGCCGC AGAAATGTTA 
ATTGATACAC TAGGTGCTGG TATTGTAAAT TCAACAGATT CAAAAGGAAG AACTCCTCTA 
CATGCAGCAG CTTTTACAGA TCACGTTGAG TGTCTGCAGC TTCTGCTTGG TCACAATGCT 
CAAGTAAATG CTGTAGATTC TTCTGGGAAA ACACCTTTAA TGATGGCTGC AGAAAATGGA 
CAGACGAATA CAGTTGAGGT GCTGGTTAGC AGTGCTAAGG CTGATCTGAC TTTGCAAGAC 
CGTTCCAAGA ACACAGCACT CCATTTGGCT TGCAGCAAGG GTCATGAAAC TAGTGCCTTG 
TTAATACTGG AGAAGATTAC GGATAGAAAC CTCATCAATG CCACCAATGC AGCCTTGCAA 
ACACCTCTGC ATGTTGCTGC TCGAAATGGA CTAACGGTTG TAGTTCAAGA GCTTTTAGGG 
AAGGGAGCAA GTGTACTTGC TGTAGATGAA AATGGTTACA CCCCGGCTCT GGCCTGCGCG 
CCCAACAAAG ACGTGGCCGA TTGCCTGGCT CTCATTCTGG CCACCATGAT GCCTGTCTCA 
TCCAGCAGCA CCTTACCTTC CCTGACGTTC AATGCCATTA ACCATTACAC CAACACCTCA 
AAAACTGTCA CCTTCGACTC CTTGCCAATC ATGAGGAGTG ACCATAGCTC TTACTGTACT 
TTCAACAACA TTGGCAGGGA AGGTGGCTAC CCCTACACAG AAGAAGATGA GCTCAATGAC 
TCAGATTCTG AGACCTATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009067447.1
CDS	1..3261
Translation	MSRVCIVVLEEVEDDSPTFISDLPQENVPLHPPPSGNVLVRHPPLVQAIFNGDPDEVRALIFKKEDVNFQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDSKWLTPLHRAVASCSEDAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVAHTAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELIDCGANVNQVNEKGFTPLHFAAASTHGALCLELLVCNGADVNIKSKDGKTPLHMTAIHGRFSRSQTIIQNGAEIDCEDKNGNTPLHIAARYGHELLINTLITSGADTAKRGIHGMFPLHLAALSGFSDCCRKLLSSGFDIDTPDDFGRTCLHAAAAGGNLECLNLLLNTGADFNKKDRFGRTPLHYAAANCNYQCLFALVGSGASVNDLDERGCTPLHYAAASDTDGKCLEYLLRNDANPGIRDKQGYNAVHYSAAYGHRLCLELIASETPLDVLMETSGTDMLNDSDNRAPISPLHLAAYHGHHQALEVLVQSLLDLDVRNNNGRTPLDLAAFKGHVECVDVLINQGASILVKDYVVKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHGAKSLLRDCRGRTPIHLSAACGHIGVLGALLQSATSVDAVPAIADNHGYTSLHWACYNGHDSCVELLLEQEVFQKMEGNSFSPLHCAVINDNEGAAEMLIDTLGAGIVNSTDSKGRTPLHAAAFTDHVECLQLLLGHNAQVNAVDSSGKTPLMMAAENGQTNTVEVLVSSAKADLTLQDRSKNTALHLACSKGHETSALLILEKITDRNLINATNAALQTPLHVAARNGLTVVVQELLGKGASVLAVDENGYTPALACAPNKDVADCLALILATMMPVSSSSTLPSLTFNAINHYTNTSKTVTFDSLPIMRSDHSSYCTFNNIGREGGYPYTEEDELNDSDSETY

Target ORF information:

RefSeq Version	XM_009069199.1
Organism	Acanthisitta chloris(rifleman)
Definition	Acanthisitta chloris ankyrin repeat domain 28 (ANKRD28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009069199.1

ORF Insert Sequence:

ATGAGTCGAG TCTGTATTGT TGTTTTGGAG GAGGTGGAAG ATGATTCTCC CACATTTATT 
TCCGATTTAC CTCAGGAAAA TGTACCTTTA CATCCACCAC CCTCTGGAAA TGTGCTGGTA 
AGGCATCCAC CATTGGTTCA AGCTATATTT AATGGAGATC CTGATGAAGT TCGAGCACTA 
ATATTTAAGA AAGAAGATGT TAATTTTCAG GATAATGAGA AAAGGACCCC TTTACATGCT 
GCTGCCTATC TGGGAGATGC AGAAATTATT GAACTACTTA TTTTATCTGG AGCTAGAGTT 
AATGCCAAAG ACAGCAAATG GTTGACTCCT TTACACAGAG CCGTAGCATC TTGTAGTGAG 
GATGCTGTTC AGGTGTTGTT AAAGCATTCA GCAGATGTCA ATGCTCGTGA CAAAAACTGG 
CAGACACCCC TACATATAGC AGCTGCAAAC AAAGCTGTGA AATGTGCTGA AGCTTTGGTG 
CCTCTCCTAA GCAATGTAAA TGTGTCTGAT CGAGCAGGCA GAACTGCATT GCATCATGCA 
GCCTTCAGTG GACACGTTGA GATGGTCAGC TTACTGTTGT CTAGAGGGGC CAATATTAAT 
GCTTTTGACA AGAAGGACAG ACGAGCTATA CATTGGGCAG CATATATGGG TCACATTGAA 
GTTGTGAAAT TACTTGTGGC ACATACAGCT GAAGTGACAT GCAAAGACAA GAAATCATAT 
ACACCCTTAC ATGCTGCAGC ATCTAGTGGG ATGATCAGTG TAGTCAAGTA TCTTCTAGAT 
CTTGGAGTTG ATATGAACGA ACCAAATGCC TATGGAAATA CTCCTCTCCA TGTAGCTTGC 
TACAACGGTC AAGATGTTGT AGTGAATGAA CTCATAGACT GCGGTGCTAA TGTAAATCAA 
GTGAATGAGA AGGGGTTTAC ACCTTTGCAC TTTGCTGCTG CATCGACACA TGGAGCATTG 
TGTTTAGAGC TTTTGGTCTG CAACGGAGCA GATGTAAATA TAAAGAGTAA AGATGGGAAA 
ACACCTTTGC ACATGACAGC GATCCATGGT AGATTTTCAA GATCCCAAAC CATCATTCAA 
AATGGAGCTG AAATAGACTG TGAAGATAAG AATGGAAATA CTCCTTTGCA CATAGCAGCT 
AGATATGGCC ACGAGCTATT AATCAACACT TTGATTACAA GTGGTGCTGA CACTGCAAAG 
CGGGGTATAC ATGGGATGTT TCCTCTCCAT TTGGCAGCAT TAAGTGGATT TTCAGACTGC 
TGCAGAAAGC TCCTCTCATC AGGTTTTGAC ATTGACACAC CAGATGACTT TGGCAGGACT 
TGTCTTCACG CAGCTGCAGC TGGAGGGAAT TTGGAGTGCC TAAATCTTCT GCTAAATACT 
GGTGCAGACT TCAACAAAAA GGACAGATTT GGGAGAACTC CACTCCATTA TGCTGCTGCC 
AATTGTAATT ATCAGTGCCT GTTTGCCCTT GTGGGATCTG GAGCTAGTGT GAATGACCTT 
GATGAGAGGG GTTGTACACC TCTGCACTAT GCAGCTGCAT CAGACACAGA TGGGAAGTGC 
CTGGAATACT TGCTAAGAAA CGACGCCAAC CCGGGGATCC GAGACAAACA AGGATACAAT 
GCAGTTCATT ATTCAGCTGC TTATGGTCAT CGCTTATGCC TTGAATTGAT TGCAAGCGAA 
ACACCTCTGG ATGTTCTAAT GGAAACATCA GGTACTGACA TGCTGAATGA TTCAGACAAC 
AGAGCACCCA TAAGTCCGCT GCACTTAGCT GCCTATCATG GCCACCATCA AGCTCTGGAA 
GTGCTGGTGC AGTCACTGCT GGACCTTGAT GTGAGGAATA ACAATGGAAG GACACCACTG 
GATCTTGCTG CCTTTAAGGG ACACGTGGAA TGTGTAGATG TGCTCATTAA TCAGGGAGCA 
TCCATCCTGG TGAAAGATTA TGTTGTGAAG AGGACCCCAA TACATGCTGC AGCTACAAAT 
GGCCATTCAG AATGTTTGCG GCTATTGATA GGAAATGCAG AACCACAGAA TGCAGTGGAC 
ATTCAAGATG GAAATGGACA GACTCCTCTG ATGTTGTCAG TTCTCAACGG GCACACAGAC 
TGTGTTTATT CACTCCTCAA CAAAGGAGCA AATGTAGATG CCAAAGATAA GTGGGGAAGG 
ACAGCATTAC ACAGAGGGGC AGTTACTGGG CATGAGGAAT GTGTGGAAGC ATTGCTTCAA 
CATGGTGCTA AGTCCTTATT ACGAGACTGT AGGGGACGAA CCCCTATACA CCTGTCAGCT 
GCATGTGGCC ATATAGGTGT TCTGGGAGCT CTCCTGCAGT CAGCTACATC TGTGGATGCA 
GTGCCTGCAA TAGCAGACAA TCACGGCTAT ACATCACTGC ATTGGGCCTG CTATAATGGT 
CATGACAGTT GTGTAGAGCT GCTCCTGGAA CAGGAAGTTT TTCAGAAAAT GGAAGGAAAT 
TCTTTCAGTC CCTTGCATTG TGCTGTGATA AATGACAATG AAGGTGCCGC AGAAATGTTA 
ATTGATACAC TAGGTGCTGG TATTGTAAAT TCAACAGATT CAAAAGGAAG AACTCCTCTA 
CATGCAGCAG CTTTTACAGA TCACGTTGAG TGTCTGCAGC TTCTGCTTGG TCACAATGCT 
CAAGTAAATG CTGTAGATTC TTCTGGGAAA ACACCTTTAA TGATGGCTGC AGAAAATGGA 
CAGACGAATA CAGTTGAGGT GCTGGTTAGC AGTGCTAAGG CTGATCTGAC TTTGCAAGAC 
CGTTCCAAGA ACACAGCACT CCATTTGGCT TGCAGCAAGG GTCATGAAAC TAGTGCCTTG 
TTAATACTGG AGAAGATTAC GGATAGAAAC CTCATCAATG CCACCAATGC AGCCTTGCAA 
ACACCTCTGC ATGTTGCTGC TCGAAATGGA CTAACGGTTG TAGTTCAAGA GCTTTTAGGG 
AAGGGAGCAA GTGTACTTGC TGTAGATGAA AATGGTTACA CCCCGGCTCT GGCCTGCGCG 
CCCAACAAAG ACGTGGCCGA TTGCCTGGCT CTCATTCTGG CCACCATGAT GCCTGTCTCA 
TCCAGCAGCA CCTTACCTTC CCTGACGTTC AATGCCATTA ACCATTACAC CAACACCTCA 
AAAACTGTCA CCTTCGACTC CTTGCCAATC ATGAGGAGTG ACCATAGCTC TTACTGTACT 
TTCAACAACA TTGGCAGGGA AGGTGGCTAC CCCTACACAG AAGAAGATGA GCTCAATGAC 
TCAGATTCTG AGACCTATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD28 ( XM_009069201.1 ) cDNA ORF clone, Acanthisitta chloris(rifleman) -> XP_009067449.1 Acanthisitta chloris ankyrin repeat domain 28 (ANKRD28), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc116503
Clone ID Related Accession (Same CDS sequence)	XM_009069201.1
Accession Version	XM_009069201.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-04
Organism	Acanthisitta chloris(rifleman)
Product	serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008658925.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthisitta chloris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAG TCTGTATTGT TGTTTTGGAG GAGGTGGAAG ATGATTCTCC CACATTTATT 
TCCGATTTAC CTCAGGAAAA TGTACCTTTA CATCCACCAC CCTCTGGAAA TGTGCTGCCA 
CCATTGGTTC AAGCTATATT TAATGGAGAT CCTGATGAAG TTCGAGCACT AATATTTAAG 
AAAGAAGATG TTAATTTTCA GGATAATGAG AAAAGGACCC CTTTACATGC TGCTGCCTAT 
CTGGGAGATG CAGAAATTAT TGAACTACTT ATTTTATCTG GAGCTAGAGT TAATGCCAAA 
GACAGCAAAT GGTTGACTCC TTTACACAGA GCCGTAGCAT CTTGTAGTGA GGATGCTGTT 
CAGGTGTTGT TAAAGCATTC AGCAGATGTC AATGCTCGTG ACAAAAACTG GCAGACACCC 
CTACATATAG CAGCTGCAAA CAAAGCTGTG AAATGTGCTG AAGCTTTGGT GCCTCTCCTA 
AGCAATGTAA ATGTGTCTGA TCGAGCAGGC AGAACTGCAT TGCATCATGC AGCCTTCAGT 
GGACACGTTG AGATGGTCAG CTTACTGTTG TCTAGAGGGG CCAATATTAA TGCTTTTGAC 
AAGAAGGACA GACGAGCTAT ACATTGGGCA GCATATATGG GTCACATTGA AGTTGTGAAA 
TTACTTGTGG CACATACAGC TGAAGTGACA TGCAAAGACA AGAAATCATA TACACCCTTA 
CATGCTGCAG CATCTAGTGG GATGATCAGT GTAGTCAAGT ATCTTCTAGA TCTTGGAGTT 
GATATGAACG AACCAAATGC CTATGGAAAT ACTCCTCTCC ATGTAGCTTG CTACAACGGT 
CAAGATGTTG TAGTGAATGA ACTCATAGAC TGCGGTGCTA ATGTAAATCA AGTGAATGAG 
AAGGGGTTTA CACCTTTGCA CTTTGCTGCT GCATCGACAC ATGGAGCATT GTGTTTAGAG 
CTTTTGGTCT GCAACGGAGC AGATGTAAAT ATAAAGAGTA AAGATGGGAA AACACCTTTG 
CACATGACAG CGATCCATGG TAGATTTTCA AGATCCCAAA CCATCATTCA AAATGGAGCT 
GAAATAGACT GTGAAGATAA GAATGGAAAT ACTCCTTTGC ACATAGCAGC TAGATATGGC 
CACGAGCTAT TAATCAACAC TTTGATTACA AGTGGTGCTG ACACTGCAAA GCGGGGTATA 
CATGGGATGT TTCCTCTCCA TTTGGCAGCA TTAAGTGGAT TTTCAGACTG CTGCAGAAAG 
CTCCTCTCAT CAGGTTTTGA CATTGACACA CCAGATGACT TTGGCAGGAC TTGTCTTCAC 
GCAGCTGCAG CTGGAGGGAA TTTGGAGTGC CTAAATCTTC TGCTAAATAC TGGTGCAGAC 
TTCAACAAAA AGGACAGATT TGGGAGAACT CCACTCCATT ATGCTGCTGC CAATTGTAAT 
TATCAGTGCC TGTTTGCCCT TGTGGGATCT GGAGCTAGTG TGAATGACCT TGATGAGAGG 
GGTTGTACAC CTCTGCACTA TGCAGCTGCA TCAGACACAG ATGGGAAGTG CCTGGAATAC 
TTGCTAAGAA ACGACGCCAA CCCGGGGATC CGAGACAAAC AAGGATACAA TGCAGTTCAT 
TATTCAGCTG CTTATGGTCA TCGCTTATGC CTTGAATTGA TTGCAAGCGA AACACCTCTG 
GATGTTCTAA TGGAAACATC AGGTACTGAC ATGCTGAATG ATTCAGACAA CAGAGCACCC 
ATAAGTCCGC TGCACTTAGC TGCCTATCAT GGCCACCATC AAGCTCTGGA AGTGCTGGTG 
CAGTCACTGC TGGACCTTGA TGTGAGGAAT AACAATGGAA GGACACCACT GGATCTTGCT 
GCCTTTAAGG GACACGTGGA ATGTGTAGAT GTGCTCATTA ATCAGGGAGC ATCCATCCTG 
GTGAAAGATT ATGTTGTGAA GAGGACCCCA ATACATGCTG CAGCTACAAA TGGCCATTCA 
GAATGTTTGC GGCTATTGAT AGGAAATGCA GAACCACAGA ATGCAGTGGA CATTCAAGAT 
GGAAATGGAC AGACTCCTCT GATGTTGTCA GTTCTCAACG GGCACACAGA CTGTGTTTAT 
TCACTCCTCA ACAAAGGAGC AAATGTAGAT GCCAAAGATA AGTGGGGAAG GACAGCATTA 
CACAGAGGGG CAGTTACTGG GCATGAGGAA TGTGTGGAAG CATTGCTTCA ACATGGTGCT 
AAGTCCTTAT TACGAGACTG TAGGGGACGA ACCCCTATAC ACCTGTCAGC TGCATGTGGC 
CATATAGGTG TTCTGGGAGC TCTCCTGCAG TCAGCTACAT CTGTGGATGC AGTGCCTGCA 
ATAGCAGACA ATCACGGCTA TACATCACTG CATTGGGCCT GCTATAATGG TCATGACAGT 
TGTGTAGAGC TGCTCCTGGA ACAGGAAGTT TTTCAGAAAA TGGAAGGAAA TTCTTTCAGT 
CCCTTGCATT GTGCTGTGAT AAATGACAAT GAAGGTGCCG CAGAAATGTT AATTGATACA 
CTAGGTGCTG GTATTGTAAA TTCAACAGAT TCAAAAGGAA GAACTCCTCT ACATGCAGCA 
GCTTTTACAG ATCACGTTGA GTGTCTGCAG CTTCTGCTTG GTCACAATGC TCAAGTAAAT 
GCTGTAGATT CTTCTGGGAA AACACCTTTA ATGATGGCTG CAGAAAATGG ACAGACGAAT 
ACAGTTGAGG TGCTGGTTAG CAGTGCTAAG GCTGATCTGA CTTTGCAAGA CCGTTCCAAG 
AACACAGCAC TCCATTTGGC TTGCAGCAAG GGTCATGAAA CTAGTGCCTT GTTAATACTG 
GAGAAGATTA CGGATAGAAA CCTCATCAAT GCCACCAATG CAGCCTTGCA AACACCTCTG 
CATGTTGCTG CTCGAAATGG ACTAACGGTT GTAGTTCAAG AGCTTTTAGG GAAGGGAGCA 
AGTGTACTTG CTGTAGATGA AAATGGTTAC ACCCCGGCTC TGGCCTGCGC GCCCAACAAA 
GACGTGGCCG ATTGCCTGGC TCTCATTCTG GCCACCATGA TGCCTGTCTC ATCCAGCAGC 
ACCTTACCTT CCCTGACGTT CAATGCCATT AACCATTACA CCAACACCTC AAAAACTGTC 
ACCTTCGACT CCTTGCCAAT CATGAGGAGT GACCATAGCT CTTACTGTAC TTTCAACAAC 
ATTGGCAGGG AAGGTGGCTA CCCCTACACA GAAGAAGATG AGCTCAATGA CTCAGATTCT 
GAGACCTATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009067449.1
CDS	1..3252
Translation	MSRVCIVVLEEVEDDSPTFISDLPQENVPLHPPPSGNVLPPLVQAIFNGDPDEVRALIFKKEDVNFQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDSKWLTPLHRAVASCSEDAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVAHTAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELIDCGANVNQVNEKGFTPLHFAAASTHGALCLELLVCNGADVNIKSKDGKTPLHMTAIHGRFSRSQTIIQNGAEIDCEDKNGNTPLHIAARYGHELLINTLITSGADTAKRGIHGMFPLHLAALSGFSDCCRKLLSSGFDIDTPDDFGRTCLHAAAAGGNLECLNLLLNTGADFNKKDRFGRTPLHYAAANCNYQCLFALVGSGASVNDLDERGCTPLHYAAASDTDGKCLEYLLRNDANPGIRDKQGYNAVHYSAAYGHRLCLELIASETPLDVLMETSGTDMLNDSDNRAPISPLHLAAYHGHHQALEVLVQSLLDLDVRNNNGRTPLDLAAFKGHVECVDVLINQGASILVKDYVVKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHGAKSLLRDCRGRTPIHLSAACGHIGVLGALLQSATSVDAVPAIADNHGYTSLHWACYNGHDSCVELLLEQEVFQKMEGNSFSPLHCAVINDNEGAAEMLIDTLGAGIVNSTDSKGRTPLHAAAFTDHVECLQLLLGHNAQVNAVDSSGKTPLMMAAENGQTNTVEVLVSSAKADLTLQDRSKNTALHLACSKGHETSALLILEKITDRNLINATNAALQTPLHVAARNGLTVVVQELLGKGASVLAVDENGYTPALACAPNKDVADCLALILATMMPVSSSSTLPSLTFNAINHYTNTSKTVTFDSLPIMRSDHSSYCTFNNIGREGGYPYTEEDELNDSDSETY

Target ORF information:

RefSeq Version	XM_009069201.1
Organism	Acanthisitta chloris(rifleman)
Definition	Acanthisitta chloris ankyrin repeat domain 28 (ANKRD28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009069201.1

ORF Insert Sequence:

ATGAGTCGAG TCTGTATTGT TGTTTTGGAG GAGGTGGAAG ATGATTCTCC CACATTTATT 
TCCGATTTAC CTCAGGAAAA TGTACCTTTA CATCCACCAC CCTCTGGAAA TGTGCTGCCA 
CCATTGGTTC AAGCTATATT TAATGGAGAT CCTGATGAAG TTCGAGCACT AATATTTAAG 
AAAGAAGATG TTAATTTTCA GGATAATGAG AAAAGGACCC CTTTACATGC TGCTGCCTAT 
CTGGGAGATG CAGAAATTAT TGAACTACTT ATTTTATCTG GAGCTAGAGT TAATGCCAAA 
GACAGCAAAT GGTTGACTCC TTTACACAGA GCCGTAGCAT CTTGTAGTGA GGATGCTGTT 
CAGGTGTTGT TAAAGCATTC AGCAGATGTC AATGCTCGTG ACAAAAACTG GCAGACACCC 
CTACATATAG CAGCTGCAAA CAAAGCTGTG AAATGTGCTG AAGCTTTGGT GCCTCTCCTA 
AGCAATGTAA ATGTGTCTGA TCGAGCAGGC AGAACTGCAT TGCATCATGC AGCCTTCAGT 
GGACACGTTG AGATGGTCAG CTTACTGTTG TCTAGAGGGG CCAATATTAA TGCTTTTGAC 
AAGAAGGACA GACGAGCTAT ACATTGGGCA GCATATATGG GTCACATTGA AGTTGTGAAA 
TTACTTGTGG CACATACAGC TGAAGTGACA TGCAAAGACA AGAAATCATA TACACCCTTA 
CATGCTGCAG CATCTAGTGG GATGATCAGT GTAGTCAAGT ATCTTCTAGA TCTTGGAGTT 
GATATGAACG AACCAAATGC CTATGGAAAT ACTCCTCTCC ATGTAGCTTG CTACAACGGT 
CAAGATGTTG TAGTGAATGA ACTCATAGAC TGCGGTGCTA ATGTAAATCA AGTGAATGAG 
AAGGGGTTTA CACCTTTGCA CTTTGCTGCT GCATCGACAC ATGGAGCATT GTGTTTAGAG 
CTTTTGGTCT GCAACGGAGC AGATGTAAAT ATAAAGAGTA AAGATGGGAA AACACCTTTG 
CACATGACAG CGATCCATGG TAGATTTTCA AGATCCCAAA CCATCATTCA AAATGGAGCT 
GAAATAGACT GTGAAGATAA GAATGGAAAT ACTCCTTTGC ACATAGCAGC TAGATATGGC 
CACGAGCTAT TAATCAACAC TTTGATTACA AGTGGTGCTG ACACTGCAAA GCGGGGTATA 
CATGGGATGT TTCCTCTCCA TTTGGCAGCA TTAAGTGGAT TTTCAGACTG CTGCAGAAAG 
CTCCTCTCAT CAGGTTTTGA CATTGACACA CCAGATGACT TTGGCAGGAC TTGTCTTCAC 
GCAGCTGCAG CTGGAGGGAA TTTGGAGTGC CTAAATCTTC TGCTAAATAC TGGTGCAGAC 
TTCAACAAAA AGGACAGATT TGGGAGAACT CCACTCCATT ATGCTGCTGC CAATTGTAAT 
TATCAGTGCC TGTTTGCCCT TGTGGGATCT GGAGCTAGTG TGAATGACCT TGATGAGAGG 
GGTTGTACAC CTCTGCACTA TGCAGCTGCA TCAGACACAG ATGGGAAGTG CCTGGAATAC 
TTGCTAAGAA ACGACGCCAA CCCGGGGATC CGAGACAAAC AAGGATACAA TGCAGTTCAT 
TATTCAGCTG CTTATGGTCA TCGCTTATGC CTTGAATTGA TTGCAAGCGA AACACCTCTG 
GATGTTCTAA TGGAAACATC AGGTACTGAC ATGCTGAATG ATTCAGACAA CAGAGCACCC 
ATAAGTCCGC TGCACTTAGC TGCCTATCAT GGCCACCATC AAGCTCTGGA AGTGCTGGTG 
CAGTCACTGC TGGACCTTGA TGTGAGGAAT AACAATGGAA GGACACCACT GGATCTTGCT 
GCCTTTAAGG GACACGTGGA ATGTGTAGAT GTGCTCATTA ATCAGGGAGC ATCCATCCTG 
GTGAAAGATT ATGTTGTGAA GAGGACCCCA ATACATGCTG CAGCTACAAA TGGCCATTCA 
GAATGTTTGC GGCTATTGAT AGGAAATGCA GAACCACAGA ATGCAGTGGA CATTCAAGAT 
GGAAATGGAC AGACTCCTCT GATGTTGTCA GTTCTCAACG GGCACACAGA CTGTGTTTAT 
TCACTCCTCA ACAAAGGAGC AAATGTAGAT GCCAAAGATA AGTGGGGAAG GACAGCATTA 
CACAGAGGGG CAGTTACTGG GCATGAGGAA TGTGTGGAAG CATTGCTTCA ACATGGTGCT 
AAGTCCTTAT TACGAGACTG TAGGGGACGA ACCCCTATAC ACCTGTCAGC TGCATGTGGC 
CATATAGGTG TTCTGGGAGC TCTCCTGCAG TCAGCTACAT CTGTGGATGC AGTGCCTGCA 
ATAGCAGACA ATCACGGCTA TACATCACTG CATTGGGCCT GCTATAATGG TCATGACAGT 
TGTGTAGAGC TGCTCCTGGA ACAGGAAGTT TTTCAGAAAA TGGAAGGAAA TTCTTTCAGT 
CCCTTGCATT GTGCTGTGAT AAATGACAAT GAAGGTGCCG CAGAAATGTT AATTGATACA 
CTAGGTGCTG GTATTGTAAA TTCAACAGAT TCAAAAGGAA GAACTCCTCT ACATGCAGCA 
GCTTTTACAG ATCACGTTGA GTGTCTGCAG CTTCTGCTTG GTCACAATGC TCAAGTAAAT 
GCTGTAGATT CTTCTGGGAA AACACCTTTA ATGATGGCTG CAGAAAATGG ACAGACGAAT 
ACAGTTGAGG TGCTGGTTAG CAGTGCTAAG GCTGATCTGA CTTTGCAAGA CCGTTCCAAG 
AACACAGCAC TCCATTTGGC TTGCAGCAAG GGTCATGAAA CTAGTGCCTT GTTAATACTG 
GAGAAGATTA CGGATAGAAA CCTCATCAAT GCCACCAATG CAGCCTTGCA AACACCTCTG 
CATGTTGCTG CTCGAAATGG ACTAACGGTT GTAGTTCAAG AGCTTTTAGG GAAGGGAGCA 
AGTGTACTTG CTGTAGATGA AAATGGTTAC ACCCCGGCTC TGGCCTGCGC GCCCAACAAA 
GACGTGGCCG ATTGCCTGGC TCTCATTCTG GCCACCATGA TGCCTGTCTC ATCCAGCAGC 
ACCTTACCTT CCCTGACGTT CAATGCCATT AACCATTACA CCAACACCTC AAAAACTGTC 
ACCTTCGACT CCTTGCCAAT CATGAGGAGT GACCATAGCT CTTACTGTAC TTTCAACAAC 
ATTGGCAGGG AAGGTGGCTA CCCCTACACA GAAGAAGATG AGCTCAATGA CTCAGATTCT 
GAGACCTATT AG