ALPK3 cDNA ORF clone, Bos taurus(cattle)

The following ALPK3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALPK3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa94213 XM_015459211.1
Latest version!
Bos taurus alpha kinase 3 (ALPK3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa94213 XM_015459212.1
Latest version!
Bos taurus alpha kinase 3 (ALPK3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa94213 XM_015468475.1
Latest version!
Bos taurus alpha kinase 3 (ALPK3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa94213 XM_015468473.1
Latest version!
Bos taurus alpha kinase 3 (ALPK3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa94213 XM_015459210.1
Latest version!
Bos taurus alpha kinase 3 (ALPK3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa94213 XM_015468474.1
Latest version!
Bos taurus alpha kinase 3 (ALPK3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa94212 XM_015459209.2
Latest version!
Bos taurus alpha kinase 3 (ALPK3), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa94212 XM_015468472.1
Latest version!
Bos taurus alpha kinase 3 (ALPK3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
View Old Accession Versions >>
OBa94212 XM_015459209.1 Bos taurus alpha kinase 3 (ALPK3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa94213
    Clone ID Related Accession (Same CDS sequence) XM_015468474.1 , XM_015459211.1 , XM_015459212.1 , XM_015468475.1 , XM_015459210.1 , XM_015468473.1
    Accession Version XM_015468474.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646530.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC 
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323960.1
    CDS1027..5688
    Translation

    Target ORF information:

    RefSeq Version XM_015468474.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 3 (ALPK3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468474.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC 
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94213
    Clone ID Related Accession (Same CDS sequence) XM_015468474.1 , XM_015459211.1 , XM_015459212.1 , XM_015468475.1 , XM_015459210.1 , XM_015468473.1
    Accession Version XM_015459211.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC 
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314697.1
    CDS1027..5688
    Translation

    Target ORF information:

    RefSeq Version XM_015459211.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 3 (ALPK3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459211.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC 
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94213
    Clone ID Related Accession (Same CDS sequence) XM_015468474.1 , XM_015459211.1 , XM_015459212.1 , XM_015468475.1 , XM_015459210.1 , XM_015468473.1
    Accession Version XM_015459212.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC 
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314698.1
    CDS1904..6565
    Translation

    Target ORF information:

    RefSeq Version XM_015459212.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 3 (ALPK3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459212.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC 
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94213
    Clone ID Related Accession (Same CDS sequence) XM_015468474.1 , XM_015459211.1 , XM_015459212.1 , XM_015468475.1 , XM_015459210.1 , XM_015468473.1
    Accession Version XM_015468475.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646530.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC 
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323961.1
    CDS1904..6565
    Translation

    Target ORF information:

    RefSeq Version XM_015468475.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 3 (ALPK3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468475.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC 
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94213
    Clone ID Related Accession (Same CDS sequence) XM_015468474.1 , XM_015459211.1 , XM_015459212.1 , XM_015468475.1 , XM_015459210.1 , XM_015468473.1
    Accession Version XM_015459210.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC 
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314696.1
    CDS905..5566
    Translation

    Target ORF information:

    RefSeq Version XM_015459210.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 3 (ALPK3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459210.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC 
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94213
    Clone ID Related Accession (Same CDS sequence) XM_015468474.1 , XM_015459211.1 , XM_015459212.1 , XM_015468475.1 , XM_015459210.1 , XM_015468473.1
    Accession Version XM_015468473.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646530.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC 
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323959.1
    CDS905..5566
    Translation

    Target ORF information:

    RefSeq Version XM_015468473.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 3 (ALPK3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468473.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC 
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94212
    Clone ID Related Accession (Same CDS sequence) XM_015459209.2 , XM_015468472.1 , XM_015459209.1
    Accession Version XM_015459209.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459209.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGACGGCCG GGCCCGGGGC TATAAATAGG GGCGCGCGTC AGCCGCGGGC GAGAGAGGCG 
    GCCGGCGGCG CGGGCCTGAC GGGCAGGGGC CGGGAGCGCG GGCAGGGGCA GGAGGCGGGC
    AGGGCCGGCA GCAGCATGGG GTCGCGGAGG GCCCCCGGCC GCGGTTGGGG CTCGGGCGGA
    CGGTCTGAGG CGGGGGGCGA CGGCGAGGAC GACGGGCCCG TGTGGATCCC CAGCCCCGCC
    AGCCGCAGCT ACCTGCTCAG CGTGCGGCCT GAGACCAGGA GCACCTTCTG CTCCATCATT
    GCTCAGCTAA CAGAGGAGAC CCAGCCGCTA TTTGAGACCA CACTCAAGTC CCGGGCTGTG
    TCCGAGGACA GCGACGTCAG ATTCACCTGC ATCGTCACAG GGTACCCAGA GCCAGAGGTG
    ACCTGGTACA AGGATGACAC AGAGCTGGAC CGCTACTGTG GCTTGCCCAA ATACGAGATC
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314695.1
    CDS83..5224
    Translation

    Target ORF information:

    RefSeq Version XM_015459209.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 3 (ALPK3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459209.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGACGGCCG GGCCCGGGGC TATAAATAGG GGCGCGCGTC AGCCGCGGGC GAGAGAGGCG 
    GCCGGCGGCG CGGGCCTGAC GGGCAGGGGC CGGGAGCGCG GGCAGGGGCA GGAGGCGGGC
    AGGGCCGGCA GCAGCATGGG GTCGCGGAGG GCCCCCGGCC GCGGTTGGGG CTCGGGCGGA
    CGGTCTGAGG CGGGGGGCGA CGGCGAGGAC GACGGGCCCG TGTGGATCCC CAGCCCCGCC
    AGCCGCAGCT ACCTGCTCAG CGTGCGGCCT GAGACCAGGA GCACCTTCTG CTCCATCATT
    GCTCAGCTAA CAGAGGAGAC CCAGCCGCTA TTTGAGACCA CACTCAAGTC CCGGGCTGTG
    TCCGAGGACA GCGACGTCAG ATTCACCTGC ATCGTCACAG GGTACCCAGA GCCAGAGGTG
    ACCTGGTACA AGGATGACAC AGAGCTGGAC CGCTACTGTG GCTTGCCCAA ATACGAGATC
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94212
    Clone ID Related Accession (Same CDS sequence) XM_015459209.2 , XM_015468472.1 , XM_015459209.1
    Accession Version XM_015468472.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646530.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGACGGCCG GGCCCGGGGC TATAAATAGG GGCGCGCGTC AGCCGCGGGC GAGAGAGGCG 
    GCCGGCGGCG CGGGCCTGAC GGGCAGGGGC CGGGAGCGCG GGCAGGGGCA GGAGGCGGGC
    AGGGCCGGCA GCAGCATGGG GTCGCGGAGG GCCCCCGGCC GCGGTTGGGG CTCGGGCGGA
    CGGTCTGAGG CGGGGGGCGA CGGCGAGGAC GACGGGCCCG TGTGGATCCC CAGCCCCGCC
    AGCCGCAGCT ACCTGCTCAG CGTGCGGCCT GAGACCAGGA GCACCTTCTG CTCCATCATT
    GCTCAGCTAA CAGAGGAGAC CCAGCCGCTA TTTGAGACCA CACTCAAGTC CCGGGCTGTG
    TCCGAGGACA GCGACGTCAG ATTCACCTGC ATCGTCACAG GGTACCCAGA GCCAGAGGTG
    ACCTGGTACA AGGATGACAC AGAGCTGGAC CGCTACTGTG GCTTGCCCAA ATACGAGATC
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323958.1
    CDS16..5157
    Translation

    Target ORF information:

    RefSeq Version XM_015468472.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 3 (ALPK3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468472.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGACGGCCG GGCCCGGGGC TATAAATAGG GGCGCGCGTC AGCCGCGGGC GAGAGAGGCG 
    GCCGGCGGCG CGGGCCTGAC GGGCAGGGGC CGGGAGCGCG GGCAGGGGCA GGAGGCGGGC
    AGGGCCGGCA GCAGCATGGG GTCGCGGAGG GCCCCCGGCC GCGGTTGGGG CTCGGGCGGA
    CGGTCTGAGG CGGGGGGCGA CGGCGAGGAC GACGGGCCCG TGTGGATCCC CAGCCCCGCC
    AGCCGCAGCT ACCTGCTCAG CGTGCGGCCT GAGACCAGGA GCACCTTCTG CTCCATCATT
    GCTCAGCTAA CAGAGGAGAC CCAGCCGCTA TTTGAGACCA CACTCAAGTC CCGGGCTGTG
    TCCGAGGACA GCGACGTCAG ATTCACCTGC ATCGTCACAG GGTACCCAGA GCCAGAGGTG
    ACCTGGTACA AGGATGACAC AGAGCTGGAC CGCTACTGTG GCTTGCCCAA ATACGAGATC
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94212
    Clone ID Related Accession (Same CDS sequence) XM_015459209.2 , XM_015468472.1 , XM_015459209.1
    Accession Version XM_015459209.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGACGGCCG GGCCCGGGGC TATAAATAGG GGCGCGCGTC AGCCGCGGGC GAGAGAGGCG 
    GCCGGCGGCG CGGGCCTGAC GGGCAGGGGC CGGGAGCGCG GGCAGGGGCA GGAGGCGGGC
    AGGGCCGGCA GCAGCATGGG GTCGCGGAGG GCCCCCGGCC GCGGTTGGGG CTCGGGCGGA
    CGGTCTGAGG CGGGGGGCGA CGGCGAGGAC GACGGGCCCG TGTGGATCCC CAGCCCCGCC
    AGCCGCAGCT ACCTGCTCAG CGTGCGGCCT GAGACCAGGA GCACCTTCTG CTCCATCATT
    GCTCAGCTAA CAGAGGAGAC CCAGCCGCTA TTTGAGACCA CACTCAAGTC CCGGGCTGTG
    TCCGAGGACA GCGACGTCAG ATTCACCTGC ATCGTCACAG GGTACCCAGA GCCAGAGGTG
    ACCTGGTACA AGGATGACAC AGAGCTGGAC CGCTACTGTG GCTTGCCCAA ATACGAGATC
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314695.1
    CDS16..5157
    Translation

    Target ORF information:

    RefSeq Version XM_015459209.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 3 (ALPK3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459209.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGACGGCCG GGCCCGGGGC TATAAATAGG GGCGCGCGTC AGCCGCGGGC GAGAGAGGCG 
    GCCGGCGGCG CGGGCCTGAC GGGCAGGGGC CGGGAGCGCG GGCAGGGGCA GGAGGCGGGC
    AGGGCCGGCA GCAGCATGGG GTCGCGGAGG GCCCCCGGCC GCGGTTGGGG CTCGGGCGGA
    CGGTCTGAGG CGGGGGGCGA CGGCGAGGAC GACGGGCCCG TGTGGATCCC CAGCCCCGCC
    AGCCGCAGCT ACCTGCTCAG CGTGCGGCCT GAGACCAGGA GCACCTTCTG CTCCATCATT
    GCTCAGCTAA CAGAGGAGAC CCAGCCGCTA TTTGAGACCA CACTCAAGTC CCGGGCTGTG
    TCCGAGGACA GCGACGTCAG ATTCACCTGC ATCGTCACAG GGTACCCAGA GCCAGAGGTG
    ACCTGGTACA AGGATGACAC AGAGCTGGAC CGCTACTGTG GCTTGCCCAA ATACGAGATC
    ATGCATCAGG GCAACCGTCA CACCCTGCAG CTCTACAGGT GTCGAGAGGA AGATGCTGCC
    ATCTATCAGG CCTCTGCCCG GAACGCCAAG GGCATTGTGT CCTGCTCAGG GGTCTTGGAG
    GTGGGCACCA TGACTGAATA CAAGATCCAC CAGCGCTGGT TTGCCAAGCT GAAGCGCAAG
    GCTGCAGCAA AGATGCGGGA AATCGAGCAG AGCTGGAAGC ACGGGAAGGA GGCTGTGGGC
    GAGGCTGATG CCCTGCGCAA ACTCAGCCCT GACCGCTTCC AGCGAAAGCG GCGGCTGAGC
    GGGGCCGAGG TGCCTGTCCC CTCGGCCCCT GCCCGGGAAG TCGAAGATGG ACCCCTAGTG
    GCTTGGCAGG AGGGAGACAC CGAGCCTGGT CAGCACCCAG GTTTGGGCCT AATCAACAGT
    CTTGCTTCTG GAGAGGTGAC CACCAATGGG GAGGCTGCCC CTGAGAACGG GGAGGATGGA
    GAGCGTGGCC TGTTGACCTA CATCTGTGAG GCCATGGAGC TGGGGCCTCA GAGAGCTCCC
    AAAAAGGAGT CTGGGGCCAA GAAGAAGAAG AAAGACATGG AACCTAAGCA AGGAGTGCGG
    AAACCAGAGA TGGAGAAGGC AGCTCAAAGC CATCGTGCCT CGGAAAACCG TTTCCCCAGT
    TCAGACAAGC CTGACTCCCG TGGGACACAG GGGCCCTTGG ACGTGGAGCA GTTTCAGACC
    CGGCCCAGAG GCAGGGCTGC CCGGGGGCCT GGATCCCCTG GAGCAGATGG CACCAGGAAG
    CCAGCCTCTG GTGCAGGTAC TCCAGACCAG GCCCAGAAGA CTCAGGCCCC GGCCCCTGTC
    CCAGGCCCAG AGCCGGAAGT GTATTTCTCC TTGAAGGACA TGTACCTGGA GAGCACCCGA
    GCGGACCAGC CCCAGATGGA AGCAGAGGCC CAGACCCCTC GGGGCAGAAC ACCTGGACAG
    AGGCCCTCAC GTCGGGAATC CAGCGAAGCT GGAGGTGAGA GGGGGCCTGC TGCCCCCGGC
    CAGCCTGTGC CTTCAGCCCC CCAGCCAACC AGGCCCTTCA ACAGGAAGAG GTTTGCCCCT
    CCGAAGCTCA AAGGAGAGCC CACTGCCAGC AGCAAGCCTG ATTCCTCCCT GAGTCAAGAT
    TCGGAACCCG GGGCTCAGAG CTCGGGGAAG ACTCCATGCC AGACTTCTGC CCAAGTGCCC
    ACCCCCCCCG CGCGTCGGAG ACACAGCACC CGGGACAGCC CCCTGCAGGG ACAAGCAGGC
    CCCAGCGCTC CAGGCAAGGT CCAGGAGTCC CAGGCAACCA TAGCTCCTGC ACCGGCCGGC
    AGCAGCTCTG ATCCAGCCTC TGCTGGTCAC AGCACCTCTA GAAGTCAGGG CATCATTGAG
    CCCATGGATG CAGAAACCCA GGAGGACAGG AGAATGTCTG TTGACCAGAA AACAGGAGGT
    GAAAAGAATA CAGAGGTGGA CAGGAAGATG CAAGTGGACG GGAGGACACA GGGAGACCAA
    ACAGAAGCCT CCCAGTGGAC TCAGGCAGAT AGGATGACAC AAGTGGATGT TGTGATGCAA
    GGAAGTAGGA GATCAGAGTC GGACAGGAAT TCCCAGGAGG ACGTGATGGC ACAAGGAAGG
    GTGGGGACGC AGGCAGAAGA GAGGACCCAG GAAGATGGAT GGGTACAGCA AGATAAGGGG
    ACACAGTCAG AGGGGAGCAC TCCCTCAACC ACGAAAGGTC ATTCAGAGAA GGCGTCCATG
    ACCAGTCTCA GCCCACACTC CAGAGCCCCC AAACGCCCAC CTTCGGAGGA TCCTAGGTCT
    CCTCAGGTTA TTGAAGGCTT TGGACAGAGC CAGGACGAGT TCTCTGTCCC AGACAAACCA
    GGTTGTGTGC TCACATCTGA CAAGACACCA GGACCAGCCT CTGGGAACCA GGAGGAAGCT
    GTGCTGGGTC CCCTGGTGGG GGGCCTCGCT CCCAGGGCAC AACTGCCTCC TGAGGTCCGT
    TCGGAGCCGG TGGGAGGAGA GCGCTCTGGA GGGCTGGAGT GGTCCACTCC GGGGAGGGAC
    AAGCCAAGGG AGGGCCTGTT CCAGGGTCCG AGGGAGGAAG AGCTGGGAAG AGTGCCACCT
    GCAGCTCTGG GTGGCCGTCC TCCAGGGGGC TTAGGCCCTG AGGGGCGCCC CCTGCCCTCT
    CCTCCTGAGG CTGGCCTGAC TCGTCATTCC CAGGAGGGGC TACCCAGCAC CCCAGCTTCT
    CAGCCCAGGA GTGCAGCTGC CTTCCCGCCC TCTGAAGACA AGACCTTGCT GAGCTCTGCT
    CCCTCACTGC ACCTGGGGCC AGGGACAGCC AGCCCGAGTC ACCCTCCAGA AACTGTGGCA
    GCAAACAGCG AAGGGCCCTG CGCCAAGGTG CCAGATGCGG ACGGGAGGAC TTCGGGTCCC
    CGGACCTGTG ACCCCGGCCT CATAGACTCC CTGAAGAACT ACTTGCTTCT GCTGCTAAAG
    CTGTCCAGCT CAGAAGCAAG TGGAGGGGGC CCGGAGTCTC AGGGGGAAGC TGCCGCTGGG
    CTTCCGGCGC CCCTGCCCAC CCTGGCCCCC ACCATAGAAG TCGCTGGACT GAGTCCGCGA
    ACCTCACGGC GCATCCTGGA GCGCGTGGAG AACAACCACT TGGTGCAGAG CGCACAGACC
    CTCCTGCTGA GCCCCGGCAC CTCCCGCCGT CTCACTGGCC TCCTGGACCG CGAGGTGGAG
    GCTGGCCGGC AGGCCTTGGC TGCTGCCCGG CGCCCAGGCC CCAGCCCCCT CTCGGTCCCC
    GCTATCGTGG TTGGTGAGGC GGAGGGCCCT GGGCTGGCCT CAGGAGGATC CAGTGAGGGT
    GAGGGACAGC TTCCCCTTGA GGGGCCTGGC CTCCTGGAGG TCTCTCGGGA AAGCAGCATG
    GGTGGGCTGC TTAGGGAGGA GGGTGGTGGG CAGGCAGCCC CTGGGCAGGG CCTGCTGTCA
    ACCGAGAGCA GAGCCCAGGA GCCCTCCCCA GAGGAGGAGG CCCCGGGGGA GGCGCTCCCC
    GCGGCGACGC CCGAGGAGCT GGCTCTTGGG GCCCGGAGGA AGAGATTTCT CCCAAAGGTA
    AAAGCTGCGG GAGATGGAGA GGGCACCAAG CCTGAAGAAA GAGAGAGCCC CTCAGTCTCC
    CCCAGGGGGC CCAGGAAGGC CCTTGCACCT CGGTCCCCAG GGACTCCTGG GCGCGAGAGA
    CGCTCCCCGA CTCAAGGCAG AAAGGCAGGC ATGCTGGAGG TGCCGCGGGC AGAGGAGGAG
    TTGGCAGCAG GAGACCCAGC CCCCAGCCCC AAAGCTGGCA GTCTAGATGC TGAGCTGGCC
    CTGGAGGAAG GCAATCAGGA TGCTCTGGCC AAGTCCAGGA AGGCCAAAGA CCTGCTGAAA
    GCCCCACAGG TGATTCGGAA GATTCGGGTG GAGCAGTTTC CTGACGCCTC GGGCAGCCTG
    AAGCTCTGGT GCCAGTTCTT CAACATTCTC AGTGACTCGG TCCTGACGTG GGCCAAGGAT
    CAGCGCCCAG TGGGCGAGGT GGGCAGGAGT GCCGGGGATG AGGGGCCCGC GGCCCTGGCC
    ATCGTGCAGG CGTCCCCCGT GGACTGTGGC GTGTATCGAT GCACCATCCA CAACGAGCAC
    GGCTCAGCCT CCACCGACTT CTGCCTCAGC CCCGAGGTGC TGTCAGGATT CATCTCCAGA
    GAAGAAGGTC AAGTTGGAGA AGAGATTGAG ATGACCCCCA TGGTGTTCGC TAAGGGTCTG
    GCTGACTCTG GCTGCTGGGG GGACAAGCTC TTTGGGCGCT TGGTGAGTGA GGAACTTCGA
    GGGGGTGGAC ACGGTTGCAG CCTGAGGAAG GCCTCCCAGG CCAAGGTCAT CTACGGGCTG
    GAACCCATCT TCGAGTCAGG CCGTACGTGC GTCATCAAGG TGTCCAGCCT GCTTGTGTTC
    GGGCCCAGCA GCGAGACTTC TCTCCTGGGC AGAAACTATG ACGTCACCAT CCAGGGGTGC
    AAGATCCAGA ACATGAGCCG GGAGTACTGC AAAATCTTTG CGGCTGAAGC CCGGGCTGCT
    CCTGGCTTTG GAGAGGTGCC TGAGATCATC CCACTGTATC TGATCTACCG GCCTGCCAAC
    AACATCCCCT ATGCCACCCT GGAGGAGGAC CTGGGCCAGC CCCTAGAGTC CTACTGCTCC
    CGGGACTGGG GCTGTGCTGC GGCTCCTGCA GCGCCTAGCA GCTCTGAGGC CGTGTGGAAA
    TGCCAGACCT TCCAGCACTG GCTCTATCTG TGGACGAACG GCAGCTTCCT TGTCACAGAC
    TTGGCAGGGG TTAACTGGAA AATGACTGAT GTGCAGATTG CTACCAAACT GCGAGGGTAC
    CAAGGCCTCA AGGAGAGCTG TTTCCCCGCC CTGCTGGACC AGTTCGCCTC CTCCCACCAG
    TGCAACACCT TCTGTGAGAT GCTGGGGCTA AAACCCCTCA AGGGCCCAGA GGCCACCCAC
    CCCCAAGCCA AGGCCAAAGG CTCCAAGAGT CCATCTGCCG GCAGGAAGGG CCCCCAGCTG
    AGTCCTCAGC CCCAGAAGAA AGGCCCCCCG AGTCCCCAGG GCACCCGGAA GAGTGCCTCG
    GGTTCCAAAG CCACCCCTCA GGCCTCAGAG GTGGTTGCCA CCCAGTTATT GGGACAGTCT
    CCCATCCAAG AGGGAGCCTC CAAGGCCCAG GGCATGCGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.