ALPK3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ALPK3
Entrez Gene ID	57538
Full Name	alpha kinase 3
Synonyms	MAK,MIDORI
General protein information	Preferred Names alpha kinase 3 Names alpha-protein kinase 3 muscle alpha-kinase muscle alpha-protein kinase myocyte induction differentiation originator
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q25.3
Disorder MIM:	617608

mRNA and Protein(s)

mRNA	Protein	Name
XM_017022444.1	XP_016877933.1	alpha-protein kinase 3 isoform X1
NM_020778.4	NP_065829.3	alpha-protein kinase 3
XM_017022445.1	XP_016877934.1	alpha-protein kinase 3 isoform X2
NM_020778.5	NP_065829.4	alpha-protein kinase 3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ALPK3	XP_536201.5
Homo sapiens (human)	ALPK3	NP_065829.3
Pan troglodytes (chimpanzee)	ALPK3	XP_003314896.1
Bos taurus (cattle)	ALPK3	XP_003587599.2
Rattus norvegicus (Norway rat)	Alpk3	NP_001178824.1
Mus musculus (house mouse)	Alpk3	NP_473426.2

Gene Ontology
Bibliography

Related articles in PubMed

A Potential Oligogenic Etiology of Hypertrophic Cardiomyopathy: A Classic Single-Gene Disorder.
Li L, Bainbridge MN, Tan Y, Willerson JT, Marian AJ
Circulation research120(7)1084-1090(2017 Mar)

Exome-wide association study reveals novel susceptibility genes to sporadic dilated cardiomyopathy.
Esslinger U, Garnier S, Korniat A, Proust C, Kararigas G, M?ller-Nurasyid M, Empana JP, Morley MP, Perret C, Stark K, Bick AG, Prasad SK, Kriebel J, Li J, Tiret L, Strauch K, O'Regan DP, Marguiles KB, Seidman JG, Boutouyrie P, Lacolley P, Jouven X, Hengstenberg C, Komajda M, Hakonarson H, Isnard R, Arbustini E, Grallert H, Cook SA, Seidman CE, Regitz-Zagrosek V, Cappola TP, Charron P, Cambien F, Villard E
PloS one12(3)e0172995(2017)

ALPK3-deficient cardiomyocytes generated from patient-derived induced pluripotent stem cells and mutant human embryonic stem cells display abnormal calcium handling and establish that ALPK3 deficiency underlies familial cardiomyopathy.
Phelan DG, Anderson DJ, Howden SE, Wong RC, Hickey PF, Pope K, Wilson GR, P?bay A, Davis AM, Petrou S, Elefanty AG, Stanley EG, James PA, Macciocca I, Bahlo M, Cheung MM, Amor DJ, Elliott DA, Lockhart PJ
European heart journal37(33)2586-90(2016 Sep)

Biallelic Truncating Mutations in ALPK3 Cause Severe Pediatric Cardiomyopathy.
Almomani R, Verhagen JM, Herkert JC, Brosens E, van Spaendonck-Zwarts KY, Asimaki A, van der Zwaag PA, Frohn-Mulder IM, Bertoli-Avella AM, Boven LG, van Slegtenhorst MA, van der Smagt JJ, van IJcken WF, Timmer B, van Stuijvenberg M, Verdijk RM, Saffitz JE, du Plessis FA, Michels M, Hofstra RM, Sinke RJ, van Tintelen JP, Wessels MW, Jongbloed JD, van de Laar IM
Journal of the American College of Cardiology67(5)515-25(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ALPK3 variant segregated with hypertrophic cardiomyopathy in 3 affected members of the family.
Title: A Potential Oligogenic Etiology of Hypertrophic Cardiomyopathy: A Classic Single-Gene Disorder.

Biallelic Truncating Mutations in ALPK3 Cause Severe Pediatric Cardiomyopathy.
Title: Biallelic Truncating Mutations in ALPK3 Cause Severe Pediatric Cardiomyopathy.

The following ALPK3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALPK3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu109405	NM_020778.5 Latest version!	Homo sapiens alpha kinase 3 (ALPK3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu09106	NM_020778.4 Latest version!	Homo sapiens alpha kinase 3 (ALPK3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85576	XM_017022444.1 Latest version!	Homo sapiens alpha kinase 3 (ALPK3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85577	XM_017022445.1 Latest version!	Homo sapiens alpha kinase 3 (ALPK3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALPK3 ( NM_020778.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_065829.4 Homo sapiens alpha kinase 3 (ALPK3), mRNA.

Price & Availability↑

CloneID	OHu109405
Clone ID Related Accession (Same CDS sequence)	NM_020778.5
Accession Version	NM_020778.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	alpha-protein kinase 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY044449.1, AC012291.11, AK024829.1 and AC103741.2. On Sep 18, 2019 this sequence version replaced NM_020778.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGTCGC GGAGGGCCCC CAGCCGGGGC TGGGGCGCGG GTGGGCGGTC GGGGGCGGGG 
GGCGACGGTG AGGACGACGG CCCCGTGTGG ATCCCCAGCC CAGCCAGCCG GAGCTACCTG 
CTCAGCGTGC GGCCCGAGAC CAGCTTATCA AGCAACCGGT TGTCTCACCC CAGCTCTGGA 
AGGAGCACCT TCTGCTCCAT CATTGCTCAG CTCACAGAGG AGACCCAGCC GCTATTTGAG 
ACCACGCTCA AGTCCCGGTC TGTGTCCGAG GACAGCGACG TCAGGTTCAC CTGCATCGTC 
ACAGGATACC CAGAGCCAGA GGTGACCTGG TACAAGGATG ATACGGAGCT GGACCGCTAC 
TGTGGCTTGC CAAAATATGA GATCACTCAT CAGGGCAACC GCCACACACT GCAGCTGTAC 
AGGTGTCGAG AAGAAGATGC CGCCATCTAC CAGGCCTCTG CCCAGAACAG CAAGGGCATT 
GTGTCCTGCT CAGGGGTCCT GGAGGTGGGC ACCATGACTG AGTACAAGAT CCACCAGCGC 
TGGTTCGCCA AGTTGAAGCG CAAGGCTGCG GCAAAGCTGC GCGAGATCGA GCAGAGCTGG 
AAGCACGAGA AGGCGGTGCC TGGGGAGGTC GACACTCTGC GCAAGCTCAG CCCCGACCGC 
TTCCAGCGAA AGCGGCGATT GAGCGGGGCT CAAGCGCCGG GCCCCTCGGT CCCTACCAGG 
GAGCCTGAGG GTGGGACCCT GGCGGCTTGG CAGGAGGGAG AGACTGAGAC TGCTCAGCAC 
TCAGGTTTGG GCCTGATCAA CAGTTTTGCT TCTGGAGAAG TGACCACCAA CGGGGAGGCT 
GCCCCCGAGA ATGGAGAGGA CGGAGAGCAT GGCTTGCTGA CATACATCTG TGACGCCATG 
GAGCTGGGGC CTCAGAGAGC CCTCAAAGAG GAGAGTGGGG CCAAGAAGAA AAAGAAAGAT 
GAGGAATCCA AGCAAGGCCT GCGGAAGCCA GAGTTAGAGA AGGCAGCCCA AAGCCGCCGT 
TCTTCAGAAA ACTGCATCCC CAGCTCAGAC GAGCCTGACT CCTGTGGGAC TCAGGGGCCC 
GTGGGCGTGG AGCAGGTTCA GACCCAGCCC AGAGGCAGGG CTGCACGGGG GCCTGGGTCC 
TCTGGCACAG ATAGTACCAG GAAGCCAGCC TCTGCTGTGG GCACTCCAGA CAAGGCCCAG 
AAGGCCCCTG GCCCAGGCCC AGGCCAGGAA GTGTATTTCT CCTTGAAGGA CATGTACCTG 
GAGAACACCC AGGCAGTCAG GCCTCTTGGG GAAGAGGGAC CCCAGACCCT GAGTGTCCGG 
GCGCCTGGGG AGAGTCCCAA GGGGAAGGCA CCCCTCAGGG CTAGAAGCGA GGGGGTGCCT 
GGCGCTCCTG GCCAGCCCAC ACACTCCTTG ACCCCCCAGC CGACTAGGCC TTTCAACAGA 
AAGAGATTTG CCCCTCCAAA GCCCAAAGGA GAGGCCACCA CTGACAGCAA GCCCATTTCT 
TCTCTGAGTC AAGCTCCAGA ATGCGGGGCC CAGAGCTTAG GAAAGGCCCC ACCTCAGGCC 
TCTGTGCAGG TGCCGACGCC CCCTGCCCGG CGGAGACATG GCACCCGGGA CAGCACGTTG 
CAGGGGCAAG CAGGCCACAG GACTCCAGGA GAGGTCCTGG AATGCCAGAC AACCACGGCT 
CCTACCATGT CGGCCAGCAG CAGCTCTGAT GTAGCCTCCA TTGGGGTTAG CACTTCCGGA 
AGTCAAGGTA TCATTGAACC CATGGATATG GAAACCCAGG AGGATGGGAG AACATCTGCT 
AACCAGAGAA CTGGAAGCAA GAAGAATGTG CAGGCAGATG GGAAGATACA AGTGGATGGA 
AGGACCAGGG GAGATGGAAC ACAGACAGCC CAGAGGACAC GTGCAGATAG GAAGACGCAG 
GTGGATGCTG GGACACAAGA AAGCAAGAGG CCACAGTCAG ACAGGAGTGC ACAGAAGGGC 
ATGATGACAC AGGGAAGGGC AGAGACACAG CTAGAAACAA CACAGGCAGG TGAGAAGATA 
CAGGAAGACA GGAAGGCCCA GGCAGATAAG GGCACACAGG AAGACAGAAG GATGCAGGGA 
GAGAAGGGGA TGCAGGGAGA GAAGGGGACG CAGTCAGAGG GGAGCGCGCC CACAGCCATG 
GAAGGTCAGT CTGAGCAAGA GGTGGCAACC AGCCTCGGCC CACCATCCAG AACCCCCAAA 
CTCCCACCTA CAGCGGGTCC TAGAGCTCCT CTGAATATTG AATGTTTTGT ACAGACCCCA 
GAAGGGTCTT GTTTCCCAAA AAAACCTGGT TGCCTGCCCA GATCTGAGGA GGCAGTAGTA 
ACAGCCTCCA GGAACCATGA GCAAACTGTG CTGGGTCCCC TGTCAGGGAA CCTCATGCTC 
CCAGCACAGC CGCCCCATGA GGGGAGTGTG GAGCAGGTGG GAGGAGAGAG ATGCCGAGGG 
CCACAGTCAT CAGGCCCAGT CGAGGCCAAG CAGGAGGACA GCCCGTTCCA GTGCCCCAAG 
GAGGAGCGGC CAGGGGGAGT GCCGTGTATG GATCAGGGTG GCTGTCCTCT AGCTGGCCTG 
AGCCAGGAGG TACCCACGAT GCCTTCTCTT CCTGGAACTG GGCTGACAGC TAGCCCAAAG 
GCGGGGCCGT GTAGCACCCC GACTTCTCAG CACGGGAGCA CAGCCACCTT CCTGCCCTCT 
GAGGATCAGG TCCTGATGAG TTCTGCCCCA ACACTGCACC TGGGGCTGGG GACCCCCACT 
CAGAGTCACC CACCAGAAAC CATGGCCACC AGCAGTGAGG GGGCCTGCGC CCAGGTACCA 
GATGTGGAGG GGCGGACCCC AGGTCCCCGG AGCTGTGACC CTGGCCTCAT AGATTCCCTG 
AAGAACTACC TGCTTCTGCT GCTGAAGCTG TCCAGCACAG AGACAAGTGG AGCAGGGGGA 
GAGTCCCAGG TGGGGGCAGC CACCGGAGGT CTGGTGCCCT CAGCCACTCT GACACCCACT 
GTGGAAGTGG CTGGGCTTAG TCCCCGGACA TCGAGGCGCA TCCTGGAGCG TGTGGAGAAC 
AACCACCTGG TGCAGAGTGC ACAGACCCTG CTGCTGAGCC CCTGTACCTC CCGCCGCCTC 
ACCGGCCTCC TGGACCGTGA GGTGCAGGCT GGCCGCCAGG CCCTTGCTGC TGCCCGAGGC 
TCCTGGGGTC CTGGTCCCAG CTCCCTCACT GTCCCTGCCA TTGTGGTAGA CGAGGAGGAC 
CCTGGGCTGG CCTCAGAAGG AGCCAGTGAG GGTGAAGGAG AGGTTTCCCC TGAGGGGCCT 
GGCCTCCTGG GGGCCTCTCA GGAGAGCAGC ATGGCTGGTC GACTGGGGGA GGCGGGTGGG 
CAGGCAGCCC CTGGACAGGG GCCCTCAGCA GAGAGCATAG CCCAGGAGCC CTCCCAAGAG 
GAGAAGTTCC CAGGGGAGGC TCTGACAGGT CTCCCGGCAG CTACACCTGA GGAACTGGCT 
CTAGGGGCCC GGAGGAAGAG ATTTCTCCCT AAGGTCAGAG CAGCAGGAGA CGGGGAGGCA 
ACCACACCTG AAGAAAGGGA GAGCCCCACG GTTTCCCCCC GGGGGCCCAG GAAAAGCCTG 
GTGCCTGGGT CCCCAGGGAC TCCAGGGCGG GAGAGACGCT CCCCTACGCA GGGCAGAAAG 
GCGAGCATGC TGGAGGTGCC TCGGGCAGAG GAGGAGCTGG CGGCAGGAGA CCTGGGCCCC 
AGCCCCAAGG CCGGCGGTCT GGACACAGAG GTGGCCCTGG ATGAAGGCAA GCAGGAGACA 
CTGGCCAAGC CCAGGAAAGC CAAAGACCTG CTGAAAGCCC CACAGGTGAT CCGGAAGATT 
CGGGTGGAGC AGTTTCCTGA TGCCTCCGGT AGCCTGAAGC TGTGGTGCCA GTTTTTCAAC 
ATTCTTAGTG ACTCAGTCTT GACATGGGCC AAGGATCAGC GCCCAGTGGG CGAGGTGGGC 
AGGAGCGCAG GGGATGAGGG GCCGGCGGCC TTGGCCATCG TGCAGGCCTC CCCCGTAGAC 
TGCGGTGTGT ATCGGTGCAC CATCCACAAT GAGCACGGCT CGGCCTCCAC CGACTTCTGC 
CTCAGCCCTG AGGTGTTGTC AGGATTCATC TCCAGAGAAG AAGGTGAAGT TGGAGAAGAG 
ATTGAGATGA CCCCTATGGT GTTTGCTAAG GGTCTGGCTG ACTCTGGCTG CTGGGGGGAC 
AAGCTCTTTG GGCGACTGGT AAGCGAGGAG CTCCGAGGGG GTGGATATGG GTGTGGCCTT 
CGGAAGGCCT CCCAGGCCAA GGTCATCTAC GGGCTGGAAC CCATCTTCGA GTCGGGCCGC 
ACGTGCATCA TCAAGGTGTC CAGCCTGCTT GTGTTTGGGC CCAGCAGTGA GACTTCTCTT 
GTGGGCAGAA ACTACGACGT CACCATCCAG GGGTGCAAGA TCCAGAACAT GAGTCGGGAG 
TACTGCAAAA TCTTCGCAGC AGAAGCCCGG GCCGCGCCTG GCTTTGGGGA GGTGCCTGAG 
ATCATCCCAC TGTATCTGAT CTACCGGCCT GCAAACAATA TCCCATATGC TACCCTGGAG 
GAAGACCTGG GCAAGCCCCT GGAGTCTTAC TGTTCTCGGG AATGGGGCTG TGCTGAGGCT 
CCGACAGCAT CTGGCAGCTC TGAGGCCATG CAGAAATGCC AGACCTTCCA ACACTGGCTG 
TATCAGTGGA CAAATGGCAG CTTCCTTGTC ACAGACTTGG CAGGGGTTGA CTGGAAGATG 
ACTGATGTGC AGATTGCTAC CAAACTCCGA GGATACCAGG GCCTCAAGGA AAGCTGCTTC 
CCTGCCCTGC TGGACCGGTT CGCCTCCTCC CACCAGTGCA ATGCCTACTG TGAGCTGCTG 
GGGCTGACAC CTCTCAAGGG CCCGGAGGCG GCCCACCCCC AAGCCAAAGC CAAAGGCTCT 
AAGAGTCCAT CTGCTGGCAG GAAAGGCTCC CAGCTGAGTC CTCAGCCCCA GAAGAAAGGC 
CTCCCTAGTC CTCAGGGCAC CCGGAAGAGT GCTCCAAGTT CCAAGGCCAC CCCTCAGGCC 
TCAGAGCCAG TCACCACTCA GTTGTTGGGA CAGCCTCCCA CCCAAGAGGA GGGCTCCAAG 
GCCCAGGGCA TGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065829.4
CDS	98..5215
Translation	MGSRRAPSRGWGAGGRSGAGGDGEDDGPVWIPSPASRSYLLSVRPETSLSSNRLSHPSSGRSTFCSIIAQLTEETQPLFETTLKSRSVSEDSDVRFTCIVTGYPEPEVTWYKDDTELDRYCGLPKYEITHQGNRHTLQLYRCREEDAAIYQASAQNSKGIVSCSGVLEVGTMTEYKIHQRWFAKLKRKAAAKLREIEQSWKHEKAVPGEVDTLRKLSPDRFQRKRRLSGAQAPGPSVPTREPEGGTLAAWQEGETETAQHSGLGLINSFASGEVTTNGEAAPENGEDGEHGLLTYICDAMELGPQRALKEESGAKKKKKDEESKQGLRKPELEKAAQSRRSSENCIPSSDEPDSCGTQGPVGVEQVQTQPRGRAARGPGSSGTDSTRKPASAVGTPDKAQKAPGPGPGQEVYFSLKDMYLENTQAVRPLGEEGPQTLSVRAPGESPKGKAPLRARSEGVPGAPGQPTHSLTPQPTRPFNRKRFAPPKPKGEATTDSKPISSLSQAPECGAQSLGKAPPQASVQVPTPPARRRHGTRDSTLQGQAGHRTPGEVLECQTTTAPTMSASSSSDVASIGVSTSGSQGIIEPMDMETQEDGRTSANQRTGSKKNVQADGKIQVDGRTRGDGTQTAQRTRADRKTQVDAGTQESKRPQSDRSAQKGMMTQGRAETQLETTQAGEKIQEDRKAQADKGTQEDRRMQGEKGMQGEKGTQSEGSAPTAMEGQSEQEVATSLGPPSRTPKLPPTAGPRAPLNIECFVQTPEGSCFPKKPGCLPRSEEAVVTASRNHEQTVLGPLSGNLMLPAQPPHEGSVEQVGGERCRGPQSSGPVEAKQEDSPFQCPKEERPGGVPCMDQGGCPLAGLSQEVPTMPSLPGTGLTASPKAGPCSTPTSQHGSTATFLPSEDQVLMSSAPTLHLGLGTPTQSHPPETMATSSEGACAQVPDVEGRTPGPRSCDPGLIDSLKNYLLLLLKLSSTETSGAGGESQVGAATGGLVPSATLTPTVEVAGLSPRTSRRILERVENNHLVQSAQTLLLSPCTSRRLTGLLDREVQAGRQALAAARGSWGPGPSSLTVPAIVVDEEDPGLASEGASEGEGEVSPEGPGLLGASQESSMAGRLGEAGGQAAPGQGPSAESIAQEPSQEEKFPGEALTGLPAATPEELALGARRKRFLPKVRAAGDGEATTPEERESPTVSPRGPRKSLVPGSPGTPGRERRSPTQGRKASMLEVPRAEEELAAGDLGPSPKAGGLDTEVALDEGKQETLAKPRKAKDLLKAPQVIRKIRVEQFPDASGSLKLWCQFFNILSDSVLTWAKDQRPVGEVGRSAGDEGPAALAIVQASPVDCGVYRCTIHNEHGSASTDFCLSPEVLSGFISREEGEVGEEIEMTPMVFAKGLADSGCWGDKLFGRLVSEELRGGGYGCGLRKASQAKVIYGLEPIFESGRTCIIKVSSLLVFGPSSETSLVGRNYDVTIQGCKIQNMSREYCKIFAAEARAAPGFGEVPEIIPLYLIYRPANNIPYATLEEDLGKPLESYCSREWGCAEAPTASGSSEAMQKCQTFQHWLYQWTNGSFLVTDLAGVDWKMTDVQIATKLRGYQGLKESCFPALLDRFASSHQCNAYCELLGLTPLKGPEAAHPQAKAKGSKSPSAGRKGSQLSPQPQKKGLPSPQGTRKSAPSSKATPQASEPVTTQLLGQPPTQEEGSKAQGMR

Target ORF information:

RefSeq Version	NM_020778.5
Organism	Homo sapiens(human)
Definition	Homo sapiens alpha kinase 3 (ALPK3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020778.5

ORF Insert Sequence:

ATGGGGTCGC GGAGGGCCCC CAGCCGGGGC TGGGGCGCGG GTGGGCGGTC GGGGGCGGGG 
GGCGACGGTG AGGACGACGG CCCCGTGTGG ATCCCCAGCC CAGCCAGCCG GAGCTACCTG 
CTCAGCGTGC GGCCCGAGAC CAGCTTATCA AGCAACCGGT TGTCTCACCC CAGCTCTGGA 
AGGAGCACCT TCTGCTCCAT CATTGCTCAG CTCACAGAGG AGACCCAGCC GCTATTTGAG 
ACCACGCTCA AGTCCCGGTC TGTGTCCGAG GACAGCGACG TCAGGTTCAC CTGCATCGTC 
ACAGGATACC CAGAGCCAGA GGTGACCTGG TACAAGGATG ATACGGAGCT GGACCGCTAC 
TGTGGCTTGC CAAAATATGA GATCACTCAT CAGGGCAACC GCCACACACT GCAGCTGTAC 
AGGTGTCGAG AAGAAGATGC CGCCATCTAC CAGGCCTCTG CCCAGAACAG CAAGGGCATT 
GTGTCCTGCT CAGGGGTCCT GGAGGTGGGC ACCATGACTG AGTACAAGAT CCACCAGCGC 
TGGTTCGCCA AGTTGAAGCG CAAGGCTGCG GCAAAGCTGC GCGAGATCGA GCAGAGCTGG 
AAGCACGAGA AGGCGGTGCC TGGGGAGGTC GACACTCTGC GCAAGCTCAG CCCCGACCGC 
TTCCAGCGAA AGCGGCGATT GAGCGGGGCT CAAGCGCCGG GCCCCTCGGT CCCTACCAGG 
GAGCCTGAGG GTGGGACCCT GGCGGCTTGG CAGGAGGGAG AGACTGAGAC TGCTCAGCAC 
TCAGGTTTGG GCCTGATCAA CAGTTTTGCT TCTGGAGAAG TGACCACCAA CGGGGAGGCT 
GCCCCCGAGA ATGGAGAGGA CGGAGAGCAT GGCTTGCTGA CATACATCTG TGACGCCATG 
GAGCTGGGGC CTCAGAGAGC CCTCAAAGAG GAGAGTGGGG CCAAGAAGAA AAAGAAAGAT 
GAGGAATCCA AGCAAGGCCT GCGGAAGCCA GAGTTAGAGA AGGCAGCCCA AAGCCGCCGT 
TCTTCAGAAA ACTGCATCCC CAGCTCAGAC GAGCCTGACT CCTGTGGGAC TCAGGGGCCC 
GTGGGCGTGG AGCAGGTTCA GACCCAGCCC AGAGGCAGGG CTGCACGGGG GCCTGGGTCC 
TCTGGCACAG ATAGTACCAG GAAGCCAGCC TCTGCTGTGG GCACTCCAGA CAAGGCCCAG 
AAGGCCCCTG GCCCAGGCCC AGGCCAGGAA GTGTATTTCT CCTTGAAGGA CATGTACCTG 
GAGAACACCC AGGCAGTCAG GCCTCTTGGG GAAGAGGGAC CCCAGACCCT GAGTGTCCGG 
GCGCCTGGGG AGAGTCCCAA GGGGAAGGCA CCCCTCAGGG CTAGAAGCGA GGGGGTGCCT 
GGCGCTCCTG GCCAGCCCAC ACACTCCTTG ACCCCCCAGC CGACTAGGCC TTTCAACAGA 
AAGAGATTTG CCCCTCCAAA GCCCAAAGGA GAGGCCACCA CTGACAGCAA GCCCATTTCT 
TCTCTGAGTC AAGCTCCAGA ATGCGGGGCC CAGAGCTTAG GAAAGGCCCC ACCTCAGGCC 
TCTGTGCAGG TGCCGACGCC CCCTGCCCGG CGGAGACATG GCACCCGGGA CAGCACGTTG 
CAGGGGCAAG CAGGCCACAG GACTCCAGGA GAGGTCCTGG AATGCCAGAC AACCACGGCT 
CCTACCATGT CGGCCAGCAG CAGCTCTGAT GTAGCCTCCA TTGGGGTTAG CACTTCCGGA 
AGTCAAGGTA TCATTGAACC CATGGATATG GAAACCCAGG AGGATGGGAG AACATCTGCT 
AACCAGAGAA CTGGAAGCAA GAAGAATGTG CAGGCAGATG GGAAGATACA AGTGGATGGA 
AGGACCAGGG GAGATGGAAC ACAGACAGCC CAGAGGACAC GTGCAGATAG GAAGACGCAG 
GTGGATGCTG GGACACAAGA AAGCAAGAGG CCACAGTCAG ACAGGAGTGC ACAGAAGGGC 
ATGATGACAC AGGGAAGGGC AGAGACACAG CTAGAAACAA CACAGGCAGG TGAGAAGATA 
CAGGAAGACA GGAAGGCCCA GGCAGATAAG GGCACACAGG AAGACAGAAG GATGCAGGGA 
GAGAAGGGGA TGCAGGGAGA GAAGGGGACG CAGTCAGAGG GGAGCGCGCC CACAGCCATG 
GAAGGTCAGT CTGAGCAAGA GGTGGCAACC AGCCTCGGCC CACCATCCAG AACCCCCAAA 
CTCCCACCTA CAGCGGGTCC TAGAGCTCCT CTGAATATTG AATGTTTTGT ACAGACCCCA 
GAAGGGTCTT GTTTCCCAAA AAAACCTGGT TGCCTGCCCA GATCTGAGGA GGCAGTAGTA 
ACAGCCTCCA GGAACCATGA GCAAACTGTG CTGGGTCCCC TGTCAGGGAA CCTCATGCTC 
CCAGCACAGC CGCCCCATGA GGGGAGTGTG GAGCAGGTGG GAGGAGAGAG ATGCCGAGGG 
CCACAGTCAT CAGGCCCAGT CGAGGCCAAG CAGGAGGACA GCCCGTTCCA GTGCCCCAAG 
GAGGAGCGGC CAGGGGGAGT GCCGTGTATG GATCAGGGTG GCTGTCCTCT AGCTGGCCTG 
AGCCAGGAGG TACCCACGAT GCCTTCTCTT CCTGGAACTG GGCTGACAGC TAGCCCAAAG 
GCGGGGCCGT GTAGCACCCC GACTTCTCAG CACGGGAGCA CAGCCACCTT CCTGCCCTCT 
GAGGATCAGG TCCTGATGAG TTCTGCCCCA ACACTGCACC TGGGGCTGGG GACCCCCACT 
CAGAGTCACC CACCAGAAAC CATGGCCACC AGCAGTGAGG GGGCCTGCGC CCAGGTACCA 
GATGTGGAGG GGCGGACCCC AGGTCCCCGG AGCTGTGACC CTGGCCTCAT AGATTCCCTG 
AAGAACTACC TGCTTCTGCT GCTGAAGCTG TCCAGCACAG AGACAAGTGG AGCAGGGGGA 
GAGTCCCAGG TGGGGGCAGC CACCGGAGGT CTGGTGCCCT CAGCCACTCT GACACCCACT 
GTGGAAGTGG CTGGGCTTAG TCCCCGGACA TCGAGGCGCA TCCTGGAGCG TGTGGAGAAC 
AACCACCTGG TGCAGAGTGC ACAGACCCTG CTGCTGAGCC CCTGTACCTC CCGCCGCCTC 
ACCGGCCTCC TGGACCGTGA GGTGCAGGCT GGCCGCCAGG CCCTTGCTGC TGCCCGAGGC 
TCCTGGGGTC CTGGTCCCAG CTCCCTCACT GTCCCTGCCA TTGTGGTAGA CGAGGAGGAC 
CCTGGGCTGG CCTCAGAAGG AGCCAGTGAG GGTGAAGGAG AGGTTTCCCC TGAGGGGCCT 
GGCCTCCTGG GGGCCTCTCA GGAGAGCAGC ATGGCTGGTC GACTGGGGGA GGCGGGTGGG 
CAGGCAGCCC CTGGACAGGG GCCCTCAGCA GAGAGCATAG CCCAGGAGCC CTCCCAAGAG 
GAGAAGTTCC CAGGGGAGGC TCTGACAGGT CTCCCGGCAG CTACACCTGA GGAACTGGCT 
CTAGGGGCCC GGAGGAAGAG ATTTCTCCCT AAGGTCAGAG CAGCAGGAGA CGGGGAGGCA 
ACCACACCTG AAGAAAGGGA GAGCCCCACG GTTTCCCCCC GGGGGCCCAG GAAAAGCCTG 
GTGCCTGGGT CCCCAGGGAC TCCAGGGCGG GAGAGACGCT CCCCTACGCA GGGCAGAAAG 
GCGAGCATGC TGGAGGTGCC TCGGGCAGAG GAGGAGCTGG CGGCAGGAGA CCTGGGCCCC 
AGCCCCAAGG CCGGCGGTCT GGACACAGAG GTGGCCCTGG ATGAAGGCAA GCAGGAGACA 
CTGGCCAAGC CCAGGAAAGC CAAAGACCTG CTGAAAGCCC CACAGGTGAT CCGGAAGATT 
CGGGTGGAGC AGTTTCCTGA TGCCTCCGGT AGCCTGAAGC TGTGGTGCCA GTTTTTCAAC 
ATTCTTAGTG ACTCAGTCTT GACATGGGCC AAGGATCAGC GCCCAGTGGG CGAGGTGGGC 
AGGAGCGCAG GGGATGAGGG GCCGGCGGCC TTGGCCATCG TGCAGGCCTC CCCCGTAGAC 
TGCGGTGTGT ATCGGTGCAC CATCCACAAT GAGCACGGCT CGGCCTCCAC CGACTTCTGC 
CTCAGCCCTG AGGTGTTGTC AGGATTCATC TCCAGAGAAG AAGGTGAAGT TGGAGAAGAG 
ATTGAGATGA CCCCTATGGT GTTTGCTAAG GGTCTGGCTG ACTCTGGCTG CTGGGGGGAC 
AAGCTCTTTG GGCGACTGGT AAGCGAGGAG CTCCGAGGGG GTGGATATGG GTGTGGCCTT 
CGGAAGGCCT CCCAGGCCAA GGTCATCTAC GGGCTGGAAC CCATCTTCGA GTCGGGCCGC 
ACGTGCATCA TCAAGGTGTC CAGCCTGCTT GTGTTTGGGC CCAGCAGTGA GACTTCTCTT 
GTGGGCAGAA ACTACGACGT CACCATCCAG GGGTGCAAGA TCCAGAACAT GAGTCGGGAG 
TACTGCAAAA TCTTCGCAGC AGAAGCCCGG GCCGCGCCTG GCTTTGGGGA GGTGCCTGAG 
ATCATCCCAC TGTATCTGAT CTACCGGCCT GCAAACAATA TCCCATATGC TACCCTGGAG 
GAAGACCTGG GCAAGCCCCT GGAGTCTTAC TGTTCTCGGG AATGGGGCTG TGCTGAGGCT 
CCGACAGCAT CTGGCAGCTC TGAGGCCATG CAGAAATGCC AGACCTTCCA ACACTGGCTG 
TATCAGTGGA CAAATGGCAG CTTCCTTGTC ACAGACTTGG CAGGGGTTGA CTGGAAGATG 
ACTGATGTGC AGATTGCTAC CAAACTCCGA GGATACCAGG GCCTCAAGGA AAGCTGCTTC 
CCTGCCCTGC TGGACCGGTT CGCCTCCTCC CACCAGTGCA ATGCCTACTG TGAGCTGCTG 
GGGCTGACAC CTCTCAAGGG CCCGGAGGCG GCCCACCCCC AAGCCAAAGC CAAAGGCTCT 
AAGAGTCCAT CTGCTGGCAG GAAAGGCTCC CAGCTGAGTC CTCAGCCCCA GAAGAAAGGC 
CTCCCTAGTC CTCAGGGCAC CCGGAAGAGT GCTCCAAGTT CCAAGGCCAC CCCTCAGGCC 
TCAGAGCCAG TCACCACTCA GTTGTTGGGA CAGCCTCCCA CCCAAGAGGA GGGCTCCAAG 
GCCCAGGGCA TGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALPK3 ( NM_020778.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065829.3 Homo sapiens alpha kinase 3 (ALPK3), mRNA.

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CloneID	OHu09106
Clone ID Related Accession (Same CDS sequence)	NM_020778.4
Accession Version	NM_020778.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Homo sapiens(human)
Product	alpha-protein kinase 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY044449.1, AC012291.11, AL833276.1, BC040192.1, BC016870.1 and AK026851.1. This sequence is a reference standard in the RefSeqGene project. On Dec 16, 2008 this sequence version replaced NM_020778.3. ##Evidence-Data-START## Transcript exon combination :: AY044449.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGTGG CTTGGCTTGT CTATGTGCTG GGCCAACAGC CACTGGCGAG GCAAGGCGAG 
GGTCAGTCAC GGCTGGTGCC AGGAAGAGGG CTGGTTCTTT GGCTCCCTGG TCTCCCGCGG 
TCTAGCCCAA GCTGGCCAGC GGTTGACCTG GCTCCCCTGG CCCCGGCCAG GCCTCGTGGA 
CCCCTCATAT GCCACACGGG ACATGAGCAG GCCGGCCGGG AGCCGGGTCC CGGGAGCTCC 
ACGAAGGGGC CTGTCCTCCA TGACCAGGAC ACCCGCTGCG CCTTCCTCCC GAGGCCTCCC 
GGGCCTCTCC AGACGCGGCG CTACTGCAGA CACCAGGGCC GCCAAGGGAG CGGACTCGGA 
GCCGGCCCTG GGGCGGGCAC ATGGGCCCCG GCGCCCCCCG GCGTCTCCAA GCCGCGCTGC 
CCGGGTCGGG CCAGGCCAGG GGAGGGACAG CAGCAGGTGA CGACGGCCCG GCCACCGGCT 
ATAAATAGGG GCGCGCGTCA GCCGCGGGCG GGAGCGGCGG CGGCGGGCAG GGGCCCGGGG 
GCCGGGGCCT GGAGGACAGG CGAGGCAGCG GCGAGTGCGG GGCCGGCGGT CGGGGAGGGC 
GGTGCCATGG GGTCGCGGAG GGCCCCCAGC CGGGGCTGGG GCGCGGGTGG GCGGTCGGGG 
GCGGGGGGCG ACGGTGAGGA CGACGGCCCC GTGTGGATCC CCAGCCCAGC CAGCCGGAGC 
TACCTGCTCA GCGTGCGGCC CGAGACCAGC TTATCAAGCA ACCGGTTGTC TCACCCCAGC 
TCTGGAAGGA GCACCTTCTG CTCCATCATT GCTCAGCTCA CAGAGGAGAC CCAGCCGCTA 
TTTGAGACCA CGCTCAAGTC CCGGTCTGTG TCCGAGGACA GCGACGTCAG GTTCACCTGC 
ATCGTCACAG GATACCCAGA GCCAGAGGTG ACCTGGTACA AGGATGATAC GGAGCTGGAC 
CGCTACTGTG GCTTGCCAAA ATATGAGATC ACTCATCAGG GCAACCGCCA CACACTGCAG 
CTGTACAGGT GTCGAGAAGA AGATGCCGCC ATCTACCAGG CCTCTGCCCA GAACAGCAAG 
GGCATTGTGT CCTGCTCAGG GGTCCTGGAG GTGGGCACCA TGACTGAGTA CAAGATCCAC 
CAGCGCTGGT TCGCCAAGTT GAAGCGCAAG GCTGCGGCAA AGCTGCGCGA GATCGAGCAG 
AGCTGGAAGC ACGAGAAGGC GGTGCCTGGG GAGGTCGACA CTCTGCGCAA GCTCAGCCCC 
GACCGCTTCC AGCGAAAGCG GCGATTGAGC GGGGCTCAAG CGCCGGGCCC CTCGGTCCCT 
ACCAGGGAGC CTGAGGGTGG GACCCTGGCG GCTTGGCAGG AGGGAGAGAC TGAGACTGCT 
CAGCACTCAG GTTTGGGCCT GATCAACAGT TTTGCTTCTG GAGAAGTGAC CACCAACGGG 
GAGGCTGCCC CCGAGAATGG AGAGGACGGA GAGCATGGCT TGCTGACATA CATCTGTGAC 
GCCATGGAGC TGGGGCCTCA GAGAGCCCTC AAAGAGGAGA GTGGGGCCAA GAAGAAAAAG 
AAAGATGAGG AATCCAAGCA AGGCCTGCGG AAGCCAGAGT TAGAGAAGGC AGCCCAAAGC 
CGCCGTTCTT CAGAAAACTG CATCCCCAGC TCAGACGAGC CTGACTCCTG TGGGACTCAG 
GGGCCCGTGG GCGTGGAGCA GGTTCAGACC CAGCCCAGAG GCAGGGCTGC ACGGGGGCCT 
GGGTCCTCTG GCACAGATAG TACCAGGAAG CCAGCCTCTG CTGTGGGCAC TCCAGACAAG 
GCCCAGAAGG CCCCTGGCCC AGGCCCAGGC CAGGAAGTGT ATTTCTCCTT GAAGGACATG 
TACCTGGAGA ACACCCAGGC AGTCAGGCCT CTTGGGGAAG AGGGACCCCA GACCCTGAGT 
GTCCGGGCGC CTGGGGAGAG TCCCAAGGGG AAGGCACCCC TCAGGGCTAG AAGCGAGGGG 
GTGCCTGGCG CTCCTGGCCA GCCCACACAC TCCTTGACCC CCCAGCCGAC TAGGCCTTTC 
AACAGAAAGA GATTTGCCCC TCCAAAGCCC AAAGGAGAGG CCACCACTGA CAGCAAGCCC 
ATTTCTTCTC TGAGTCAAGC TCCAGAATGC GGGGCCCAGA GCTTAGGAAA GGCCCCACCT 
CAGGCCTCTG TGCAGGTGCC GACGCCCCCT GCCCGGCGGA GACATGGCAC CCGGGACAGC 
ACGTTGCAGG GGCAAGCAGG CCACAGGACT CCAGGAGAGG TCCTGGAATG CCAGACAACC 
ACGGCTCCTA CCATGTCGGC CAGCAGCAGC TCTGATGTAG CCTCCATTGG GGTTAGCACT 
TCCGGAAGTC AAGGTATCAT TGAACCCATG GATATGGAAA CCCAGGAGGA TGGGAGAACA 
TCTGCTAACC AGAGAACTGG AAGCAAGAAG AATGTGCAGG CAGATGGGAA GATACAAGTG 
GATGGAAGGA CCAGGGGAGA TGGAACACAG ACAGCCCAGA GGACACGTGC AGATAGGAAG 
ACGCAGGTGG ATGCTGGGAC ACAAGAAAGC AAGAGGCCAC AGTCAGACAG GAGTGCACAG 
AAGGGCATGA TGACACAGGG AAGGGCAGAG ACACAGCTAG AAACAACACA GGCAGGTGAG 
AAGATACAGG AAGACAGGAA GGCCCAGGCA GATAAGGGCA CACAGGAAGA CAGAAGGATG 
CAGGGAGAGA AGGGGATGCA GGGAGAGAAG GGGACGCAGT CAGAGGGGAG CGCGCCCACA 
GCCATGGAAG GTCAGTCTGA GCAAGAGGTG GCAACCAGCC TCGGCCCACC ATCCAGAACC 
CCCAAACTCC CACCTACAGC GGGTCCTAGA GCTCCTCTGA ATATTGAATG TTTTGTACAG 
ACCCCAGAAG GGTCTTGTTT CCCAAAAAAA CCTGGTTGCC TGCCCAGATC TGAGGAGGCA 
GTAGTAACAG CCTCCAGGAA CCATGAGCAA ACTGTGCTGG GTCCCCTGTC AGGGAACCTC 
ATGCTCCCAG CACAGCCGCC CCATGAGGGG AGTGTGGAGC AGGTGGGAGG AGAGAGATGC 
CGAGGGCCAC AGTCATCAGG CCCAGTCGAG GCCAAGCAGG AGGACAGCCC GTTCCAGTGC 
CCCAAGGAGG AGCGGCCAGG GGGAGTGCCG TGTATGGATC AGGGTGGCTG TCCTCTAGCT 
GGCCTGAGCC AGGAGGTACC CACGATGCCT TCTCTTCCTG GAACTGGGCT GACAGCTAGC 
CCAAAGGCGG GGCCGTGTAG CACCCCGACT TCTCAGCACG GGAGCACAGC CACCTTCCTG 
CCCTCTGAGG ATCAGGTCCT GATGAGTTCT GCCCCAACAC TGCACCTGGG GCTGGGGACC 
CCCACTCAGA GTCACCCACC AGAAACCATG GCCACCAGCA GTGAGGGGGC CTGCGCCCAG 
GTACCAGATG TGGAGGGGCG GACCCCAGGT CCCCGGAGCT GTGACCCTGG CCTCATAGAT 
TCCCTGAAGA ACTACCTGCT TCTGCTGCTG AAGCTGTCCA GCACAGAGAC AAGTGGAGCA 
GGGGGAGAGT CCCAGGTGGG GGCAGCCACC GGAGGTCTGG TGCCCTCAGC CACTCTGACA 
CCCACTGTGG AAGTGGCTGG GCTTAGTCCC CGGACATCGA GGCGCATCCT GGAGCGTGTG 
GAGAACAACC ACCTGGTGCA GAGTGCACAG ACCCTGCTGC TGAGCCCCTG TACCTCCCGC 
CGCCTCACCG GCCTCCTGGA CCGTGAGGTG CAGGCTGGCC GCCAGGCCCT TGCTGCTGCC 
CGAGGCTCCT GGGGTCCTGG TCCCAGCTCC CTCACTGTCC CTGCCATTGT GGTAGACGAG 
GAGGACCCTG GGCTGGCCTC AGAAGGAGCC AGTGAGGGTG AAGGAGAGGT TTCCCCTGAG 
GGGCCTGGCC TCCTGGGGGC CTCTCAGGAG AGCAGCATGG CTGGTCGACT GGGGGAGGCG 
GGTGGGCAGG CAGCCCCTGG ACAGGGGCCC TCAGCAGAGA GCATAGCCCA GGAGCCCTCC 
CAAGAGGAGA AGTTCCCAGG GGAGGCTCTG ACAGGTCTCC CGGCAGCTAC ACCTGAGGAA 
CTGGCTCTAG GGGCCCGGAG GAAGAGATTT CTCCCTAAGG TCAGAGCAGC AGGAGACGGG 
GAGGCAACCA CACCTGAAGA AAGGGAGAGC CCCACGGTTT CCCCCCGGGG GCCCAGGAAA 
AGCCTGGTGC CTGGGTCCCC AGGGACTCCA GGGCGGGAGA GACGCTCCCC TACGCAGGGC 
AGAAAGGCGA GCATGCTGGA GGTGCCTCGG GCAGAGGAGG AGCTGGCGGC AGGAGACCTG 
GGCCCCAGCC CCAAGGCCGG CGGTCTGGAC ACAGAGGTGG CCCTGGATGA AGGCAAGCAG 
GAGACACTGG CCAAGCCCAG GAAAGCCAAA GACCTGCTGA AAGCCCCACA GGTGATCCGG 
AAGATTCGGG TGGAGCAGTT TCCTGATGCC TCCGGTAGCC TGAAGCTGTG GTGCCAGTTT 
TTCAACATTC TTAGTGACTC AGTCTTGACA TGGGCCAAGG ATCAGCGCCC AGTGGGCGAG 
GTGGGCAGGA GCGCAGGGGA TGAGGGGCCG GCGGCCTTGG CCATCGTGCA GGCCTCCCCC 
GTAGACTGCG GTGTGTATCG GTGCACCATC CACAATGAGC ACGGCTCGGC CTCCACCGAC 
TTCTGCCTCA GCCCTGAGGT GTTGTCAGGA TTCATCTCCA GAGAAGAAGG TGAAGTTGGA 
GAAGAGATTG AGATGACCCC TATGGTGTTT GCTAAGGGTC TGGCTGACTC TGGCTGCTGG 
GGGGACAAGC TCTTTGGGCG ACTGGTAAGC GAGGAGCTCC GAGGGGGTGG ATATGGGTGT 
GGCCTTCGGA AGGCCTCCCA GGCCAAGGTC ATCTACGGGC TGGAACCCAT CTTCGAGTCG 
GGCCGCACGT GCATCATCAA GGTGTCCAGC CTGCTTGTGT TTGGGCCCAG CAGTGAGACT 
TCTCTTGTGG GCAGAAACTA CGACGTCACC ATCCAGGGGT GCAAGATCCA GAACATGAGT 
CGGGAGTACT GCAAAATCTT CGCAGCAGAA GCCCGGGCCG CGCCTGGCTT TGGGGAGGTG 
CCTGAGATCA TCCCACTGTA TCTGATCTAC CGGCCTGCAA ACAATATCCC ATATGCTACC 
CTGGAGGAAG ACCTGGGCAA GCCCCTGGAG TCTTACTGTT CTCGGGAATG GGGCTGTGCT 
GAGGCTCCGA CAGCATCTGG CAGCTCTGAG GCCATGCAGA AATGCCAGAC CTTCCAACAC 
TGGCTGTATC AGTGGACAAA TGGCAGCTTC CTTGTCACAG ACTTGGCAGG GGTTGACTGG 
AAGATGACTG ATGTGCAGAT TGCTACCAAA CTCCGAGGAT ACCAGGGCCT CAAGGAAAGC 
TGCTTCCCTG CCCTGCTGGA CCGGTTCGCC TCCTCCCACC AGTGCAATGC CTACTGTGAG 
CTGCTGGGGC TGACACCTCT CAAGGGCCCG GAGGCGGCCC ACCCCCAAGC CAAAGCCAAA 
GGCTCTAAGA GTCCATCTGC TGGCAGGAAA GGCTCCCAGC TGAGTCCTCA GCCCCAGAAG 
AAAGGCCTCC CTAGTCCTCA GGGCACCCGG AAGAGTGCTC CAAGTTCCAA GGCCACCCCT 
CAGGCCTCAG AGCCAGTCAC CACTCAGTTG TTGGGACAGC CTCCCACCCA AGAGGAGGGC 
TCCAAGGCCC AGGGCATGCG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065829.3
CDS	168..5891
Translation	MEVAWLVYVLGQQPLARQGEGQSRLVPGRGLVLWLPGLPRSSPSWPAVDLAPLAPARPRGPLICHTGHEQAGREPGPGSSTKGPVLHDQDTRCAFLPRPPGPLQTRRYCRHQGRQGSGLGAGPGAGTWAPAPPGVSKPRCPGRARPGEGQQQVTTARPPAINRGARQPRAGAAAAGRGPGAGAWRTGEAAASAGPAVGEGGAMGSRRAPSRGWGAGGRSGAGGDGEDDGPVWIPSPASRSYLLSVRPETSLSSNRLSHPSSGRSTFCSIIAQLTEETQPLFETTLKSRSVSEDSDVRFTCIVTGYPEPEVTWYKDDTELDRYCGLPKYEITHQGNRHTLQLYRCREEDAAIYQASAQNSKGIVSCSGVLEVGTMTEYKIHQRWFAKLKRKAAAKLREIEQSWKHEKAVPGEVDTLRKLSPDRFQRKRRLSGAQAPGPSVPTREPEGGTLAAWQEGETETAQHSGLGLINSFASGEVTTNGEAAPENGEDGEHGLLTYICDAMELGPQRALKEESGAKKKKKDEESKQGLRKPELEKAAQSRRSSENCIPSSDEPDSCGTQGPVGVEQVQTQPRGRAARGPGSSGTDSTRKPASAVGTPDKAQKAPGPGPGQEVYFSLKDMYLENTQAVRPLGEEGPQTLSVRAPGESPKGKAPLRARSEGVPGAPGQPTHSLTPQPTRPFNRKRFAPPKPKGEATTDSKPISSLSQAPECGAQSLGKAPPQASVQVPTPPARRRHGTRDSTLQGQAGHRTPGEVLECQTTTAPTMSASSSSDVASIGVSTSGSQGIIEPMDMETQEDGRTSANQRTGSKKNVQADGKIQVDGRTRGDGTQTAQRTRADRKTQVDAGTQESKRPQSDRSAQKGMMTQGRAETQLETTQAGEKIQEDRKAQADKGTQEDRRMQGEKGMQGEKGTQSEGSAPTAMEGQSEQEVATSLGPPSRTPKLPPTAGPRAPLNIECFVQTPEGSCFPKKPGCLPRSEEAVVTASRNHEQTVLGPLSGNLMLPAQPPHEGSVEQVGGERCRGPQSSGPVEAKQEDSPFQCPKEERPGGVPCMDQGGCPLAGLSQEVPTMPSLPGTGLTASPKAGPCSTPTSQHGSTATFLPSEDQVLMSSAPTLHLGLGTPTQSHPPETMATSSEGACAQVPDVEGRTPGPRSCDPGLIDSLKNYLLLLLKLSSTETSGAGGESQVGAATGGLVPSATLTPTVEVAGLSPRTSRRILERVENNHLVQSAQTLLLSPCTSRRLTGLLDREVQAGRQALAAARGSWGPGPSSLTVPAIVVDEEDPGLASEGASEGEGEVSPEGPGLLGASQESSMAGRLGEAGGQAAPGQGPSAESIAQEPSQEEKFPGEALTGLPAATPEELALGARRKRFLPKVRAAGDGEATTPEERESPTVSPRGPRKSLVPGSPGTPGRERRSPTQGRKASMLEVPRAEEELAAGDLGPSPKAGGLDTEVALDEGKQETLAKPRKAKDLLKAPQVIRKIRVEQFPDASGSLKLWCQFFNILSDSVLTWAKDQRPVGEVGRSAGDEGPAALAIVQASPVDCGVYRCTIHNEHGSASTDFCLSPEVLSGFISREEGEVGEEIEMTPMVFAKGLADSGCWGDKLFGRLVSEELRGGGYGCGLRKASQAKVIYGLEPIFESGRTCIIKVSSLLVFGPSSETSLVGRNYDVTIQGCKIQNMSREYCKIFAAEARAAPGFGEVPEIIPLYLIYRPANNIPYATLEEDLGKPLESYCSREWGCAEAPTASGSSEAMQKCQTFQHWLYQWTNGSFLVTDLAGVDWKMTDVQIATKLRGYQGLKESCFPALLDRFASSHQCNAYCELLGLTPLKGPEAAHPQAKAKGSKSPSAGRKGSQLSPQPQKKGLPSPQGTRKSAPSSKATPQASEPVTTQLLGQPPTQEEGSKAQGMR

Target ORF information:

RefSeq Version	NM_020778.4
Organism	Homo sapiens(human)
Definition	Homo sapiens alpha kinase 3 (ALPK3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020778.4

ORF Insert Sequence:

ATGGAGGTGG CTTGGCTTGT CTATGTGCTG GGCCAACAGC CACTGGCGAG GCAAGGCGAG 
GGTCAGTCAC GGCTGGTGCC AGGAAGAGGG CTGGTTCTTT GGCTCCCTGG TCTCCCGCGG 
TCTAGCCCAA GCTGGCCAGC GGTTGACCTG GCTCCCCTGG CCCCGGCCAG GCCTCGTGGA 
CCCCTCATAT GCCACACGGG ACATGAGCAG GCCGGCCGGG AGCCGGGTCC CGGGAGCTCC 
ACGAAGGGGC CTGTCCTCCA TGACCAGGAC ACCCGCTGCG CCTTCCTCCC GAGGCCTCCC 
GGGCCTCTCC AGACGCGGCG CTACTGCAGA CACCAGGGCC GCCAAGGGAG CGGACTCGGA 
GCCGGCCCTG GGGCGGGCAC ATGGGCCCCG GCGCCCCCCG GCGTCTCCAA GCCGCGCTGC 
CCGGGTCGGG CCAGGCCAGG GGAGGGACAG CAGCAGGTGA CGACGGCCCG GCCACCGGCT 
ATAAATAGGG GCGCGCGTCA GCCGCGGGCG GGAGCGGCGG CGGCGGGCAG GGGCCCGGGG 
GCCGGGGCCT GGAGGACAGG CGAGGCAGCG GCGAGTGCGG GGCCGGCGGT CGGGGAGGGC 
GGTGCCATGG GGTCGCGGAG GGCCCCCAGC CGGGGCTGGG GCGCGGGTGG GCGGTCGGGG 
GCGGGGGGCG ACGGTGAGGA CGACGGCCCC GTGTGGATCC CCAGCCCAGC CAGCCGGAGC 
TACCTGCTCA GCGTGCGGCC CGAGACCAGC TTATCAAGCA ACCGGTTGTC TCACCCCAGC 
TCTGGAAGGA GCACCTTCTG CTCCATCATT GCTCAGCTCA CAGAGGAGAC CCAGCCGCTA 
TTTGAGACCA CGCTCAAGTC CCGGTCTGTG TCCGAGGACA GCGACGTCAG GTTCACCTGC 
ATCGTCACAG GATACCCAGA GCCAGAGGTG ACCTGGTACA AGGATGATAC GGAGCTGGAC 
CGCTACTGTG GCTTGCCAAA ATATGAGATC ACTCATCAGG GCAACCGCCA CACACTGCAG 
CTGTACAGGT GTCGAGAAGA AGATGCCGCC ATCTACCAGG CCTCTGCCCA GAACAGCAAG 
GGCATTGTGT CCTGCTCAGG GGTCCTGGAG GTGGGCACCA TGACTGAGTA CAAGATCCAC 
CAGCGCTGGT TCGCCAAGTT GAAGCGCAAG GCTGCGGCAA AGCTGCGCGA GATCGAGCAG 
AGCTGGAAGC ACGAGAAGGC GGTGCCTGGG GAGGTCGACA CTCTGCGCAA GCTCAGCCCC 
GACCGCTTCC AGCGAAAGCG GCGATTGAGC GGGGCTCAAG CGCCGGGCCC CTCGGTCCCT 
ACCAGGGAGC CTGAGGGTGG GACCCTGGCG GCTTGGCAGG AGGGAGAGAC TGAGACTGCT 
CAGCACTCAG GTTTGGGCCT GATCAACAGT TTTGCTTCTG GAGAAGTGAC CACCAACGGG 
GAGGCTGCCC CCGAGAATGG AGAGGACGGA GAGCATGGCT TGCTGACATA CATCTGTGAC 
GCCATGGAGC TGGGGCCTCA GAGAGCCCTC AAAGAGGAGA GTGGGGCCAA GAAGAAAAAG 
AAAGATGAGG AATCCAAGCA AGGCCTGCGG AAGCCAGAGT TAGAGAAGGC AGCCCAAAGC 
CGCCGTTCTT CAGAAAACTG CATCCCCAGC TCAGACGAGC CTGACTCCTG TGGGACTCAG 
GGGCCCGTGG GCGTGGAGCA GGTTCAGACC CAGCCCAGAG GCAGGGCTGC ACGGGGGCCT 
GGGTCCTCTG GCACAGATAG TACCAGGAAG CCAGCCTCTG CTGTGGGCAC TCCAGACAAG 
GCCCAGAAGG CCCCTGGCCC AGGCCCAGGC CAGGAAGTGT ATTTCTCCTT GAAGGACATG 
TACCTGGAGA ACACCCAGGC AGTCAGGCCT CTTGGGGAAG AGGGACCCCA GACCCTGAGT 
GTCCGGGCGC CTGGGGAGAG TCCCAAGGGG AAGGCACCCC TCAGGGCTAG AAGCGAGGGG 
GTGCCTGGCG CTCCTGGCCA GCCCACACAC TCCTTGACCC CCCAGCCGAC TAGGCCTTTC 
AACAGAAAGA GATTTGCCCC TCCAAAGCCC AAAGGAGAGG CCACCACTGA CAGCAAGCCC 
ATTTCTTCTC TGAGTCAAGC TCCAGAATGC GGGGCCCAGA GCTTAGGAAA GGCCCCACCT 
CAGGCCTCTG TGCAGGTGCC GACGCCCCCT GCCCGGCGGA GACATGGCAC CCGGGACAGC 
ACGTTGCAGG GGCAAGCAGG CCACAGGACT CCAGGAGAGG TCCTGGAATG CCAGACAACC 
ACGGCTCCTA CCATGTCGGC CAGCAGCAGC TCTGATGTAG CCTCCATTGG GGTTAGCACT 
TCCGGAAGTC AAGGTATCAT TGAACCCATG GATATGGAAA CCCAGGAGGA TGGGAGAACA 
TCTGCTAACC AGAGAACTGG AAGCAAGAAG AATGTGCAGG CAGATGGGAA GATACAAGTG 
GATGGAAGGA CCAGGGGAGA TGGAACACAG ACAGCCCAGA GGACACGTGC AGATAGGAAG 
ACGCAGGTGG ATGCTGGGAC ACAAGAAAGC AAGAGGCCAC AGTCAGACAG GAGTGCACAG 
AAGGGCATGA TGACACAGGG AAGGGCAGAG ACACAGCTAG AAACAACACA GGCAGGTGAG 
AAGATACAGG AAGACAGGAA GGCCCAGGCA GATAAGGGCA CACAGGAAGA CAGAAGGATG 
CAGGGAGAGA AGGGGATGCA GGGAGAGAAG GGGACGCAGT CAGAGGGGAG CGCGCCCACA 
GCCATGGAAG GTCAGTCTGA GCAAGAGGTG GCAACCAGCC TCGGCCCACC ATCCAGAACC 
CCCAAACTCC CACCTACAGC GGGTCCTAGA GCTCCTCTGA ATATTGAATG TTTTGTACAG 
ACCCCAGAAG GGTCTTGTTT CCCAAAAAAA CCTGGTTGCC TGCCCAGATC TGAGGAGGCA 
GTAGTAACAG CCTCCAGGAA CCATGAGCAA ACTGTGCTGG GTCCCCTGTC AGGGAACCTC 
ATGCTCCCAG CACAGCCGCC CCATGAGGGG AGTGTGGAGC AGGTGGGAGG AGAGAGATGC 
CGAGGGCCAC AGTCATCAGG CCCAGTCGAG GCCAAGCAGG AGGACAGCCC GTTCCAGTGC 
CCCAAGGAGG AGCGGCCAGG GGGAGTGCCG TGTATGGATC AGGGTGGCTG TCCTCTAGCT 
GGCCTGAGCC AGGAGGTACC CACGATGCCT TCTCTTCCTG GAACTGGGCT GACAGCTAGC 
CCAAAGGCGG GGCCGTGTAG CACCCCGACT TCTCAGCACG GGAGCACAGC CACCTTCCTG 
CCCTCTGAGG ATCAGGTCCT GATGAGTTCT GCCCCAACAC TGCACCTGGG GCTGGGGACC 
CCCACTCAGA GTCACCCACC AGAAACCATG GCCACCAGCA GTGAGGGGGC CTGCGCCCAG 
GTACCAGATG TGGAGGGGCG GACCCCAGGT CCCCGGAGCT GTGACCCTGG CCTCATAGAT 
TCCCTGAAGA ACTACCTGCT TCTGCTGCTG AAGCTGTCCA GCACAGAGAC AAGTGGAGCA 
GGGGGAGAGT CCCAGGTGGG GGCAGCCACC GGAGGTCTGG TGCCCTCAGC CACTCTGACA 
CCCACTGTGG AAGTGGCTGG GCTTAGTCCC CGGACATCGA GGCGCATCCT GGAGCGTGTG 
GAGAACAACC ACCTGGTGCA GAGTGCACAG ACCCTGCTGC TGAGCCCCTG TACCTCCCGC 
CGCCTCACCG GCCTCCTGGA CCGTGAGGTG CAGGCTGGCC GCCAGGCCCT TGCTGCTGCC 
CGAGGCTCCT GGGGTCCTGG TCCCAGCTCC CTCACTGTCC CTGCCATTGT GGTAGACGAG 
GAGGACCCTG GGCTGGCCTC AGAAGGAGCC AGTGAGGGTG AAGGAGAGGT TTCCCCTGAG 
GGGCCTGGCC TCCTGGGGGC CTCTCAGGAG AGCAGCATGG CTGGTCGACT GGGGGAGGCG 
GGTGGGCAGG CAGCCCCTGG ACAGGGGCCC TCAGCAGAGA GCATAGCCCA GGAGCCCTCC 
CAAGAGGAGA AGTTCCCAGG GGAGGCTCTG ACAGGTCTCC CGGCAGCTAC ACCTGAGGAA 
CTGGCTCTAG GGGCCCGGAG GAAGAGATTT CTCCCTAAGG TCAGAGCAGC AGGAGACGGG 
GAGGCAACCA CACCTGAAGA AAGGGAGAGC CCCACGGTTT CCCCCCGGGG GCCCAGGAAA 
AGCCTGGTGC CTGGGTCCCC AGGGACTCCA GGGCGGGAGA GACGCTCCCC TACGCAGGGC 
AGAAAGGCGA GCATGCTGGA GGTGCCTCGG GCAGAGGAGG AGCTGGCGGC AGGAGACCTG 
GGCCCCAGCC CCAAGGCCGG CGGTCTGGAC ACAGAGGTGG CCCTGGATGA AGGCAAGCAG 
GAGACACTGG CCAAGCCCAG GAAAGCCAAA GACCTGCTGA AAGCCCCACA GGTGATCCGG 
AAGATTCGGG TGGAGCAGTT TCCTGATGCC TCCGGTAGCC TGAAGCTGTG GTGCCAGTTT 
TTCAACATTC TTAGTGACTC AGTCTTGACA TGGGCCAAGG ATCAGCGCCC AGTGGGCGAG 
GTGGGCAGGA GCGCAGGGGA TGAGGGGCCG GCGGCCTTGG CCATCGTGCA GGCCTCCCCC 
GTAGACTGCG GTGTGTATCG GTGCACCATC CACAATGAGC ACGGCTCGGC CTCCACCGAC 
TTCTGCCTCA GCCCTGAGGT GTTGTCAGGA TTCATCTCCA GAGAAGAAGG TGAAGTTGGA 
GAAGAGATTG AGATGACCCC TATGGTGTTT GCTAAGGGTC TGGCTGACTC TGGCTGCTGG 
GGGGACAAGC TCTTTGGGCG ACTGGTAAGC GAGGAGCTCC GAGGGGGTGG ATATGGGTGT 
GGCCTTCGGA AGGCCTCCCA GGCCAAGGTC ATCTACGGGC TGGAACCCAT CTTCGAGTCG 
GGCCGCACGT GCATCATCAA GGTGTCCAGC CTGCTTGTGT TTGGGCCCAG CAGTGAGACT 
TCTCTTGTGG GCAGAAACTA CGACGTCACC ATCCAGGGGT GCAAGATCCA GAACATGAGT 
CGGGAGTACT GCAAAATCTT CGCAGCAGAA GCCCGGGCCG CGCCTGGCTT TGGGGAGGTG 
CCTGAGATCA TCCCACTGTA TCTGATCTAC CGGCCTGCAA ACAATATCCC ATATGCTACC 
CTGGAGGAAG ACCTGGGCAA GCCCCTGGAG TCTTACTGTT CTCGGGAATG GGGCTGTGCT 
GAGGCTCCGA CAGCATCTGG CAGCTCTGAG GCCATGCAGA AATGCCAGAC CTTCCAACAC 
TGGCTGTATC AGTGGACAAA TGGCAGCTTC CTTGTCACAG ACTTGGCAGG GGTTGACTGG 
AAGATGACTG ATGTGCAGAT TGCTACCAAA CTCCGAGGAT ACCAGGGCCT CAAGGAAAGC 
TGCTTCCCTG CCCTGCTGGA CCGGTTCGCC TCCTCCCACC AGTGCAATGC CTACTGTGAG 
CTGCTGGGGC TGACACCTCT CAAGGGCCCG GAGGCGGCCC ACCCCCAAGC CAAAGCCAAA 
GGCTCTAAGA GTCCATCTGC TGGCAGGAAA GGCTCCCAGC TGAGTCCTCA GCCCCAGAAG 
AAAGGCCTCC CTAGTCCTCA GGGCACCCGG AAGAGTGCTC CAAGTTCCAA GGCCACCCCT 
CAGGCCTCAG AGCCAGTCAC CACTCAGTTG TTGGGACAGC CTCCCACCCA AGAGGAGGGC 
TCCAAGGCCC AGGGCATGCG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALPK3 ( XM_017022444.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877933.1 Homo sapiens alpha kinase 3 (ALPK3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu85576
Clone ID Related Accession (Same CDS sequence)	XM_017022444.1
Accession Version	XM_017022444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	alpha-protein kinase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGG CTTGGCTTGT CTATGTGCTG GGCCAACAGC CACTGGCGAG GCAAGGCGAG 
GGTCAGTCAC GGCTGGTGCC AGGAAGAGGG CTGGTTCTTT GGCTCCCTGG TCTCCCGCGG 
TCTAGCCCAA GCTGGCCAGC GGTTGACCTG GCTCCCCTGG CCCCGGCCAG GCCTCGTGGA 
CCCCTCATAT GCCACACGGG ACATGAGCAG GCCGGCCGGG AGCCGGGTCC CGGGAGCTCC 
ACGAAGGGGC CTGTCCTCCA TGACCAGGAC ACCCGCTGCG CCTTCCTCCC GAGGCCTCCC 
GGGCCTCTCC AGACGCGGCG CTACTGCAGA CACCAGGGCC GCCAAGGGAG CGGACTCGGA 
GCCGGCCCTG GGGCGGGCAC ATGGGCCCCG GCGCCCCCCG GCGTCTCCAA GCCGCGCTGC 
CCGGGTCGGG CCAGGCCAGG GGAGGGACAG CAGCAGGTGA CGACGGCCCG GCCACCGGCT 
ATAAATAGGG GCGCGCGTCA GCCGCGGGCG GGAGCGGCGG CGGCGGGCAG GGGCCCGGGG 
GCCGGGGCCT GGAGGACAGG CGAGGCAGCG GCGAGTGCGG GGCCGGCGGT CGGGGAGGGC 
GGTGCCATGG GGTCGCGGAG GGCCCCCAGC CGGGGCTGGG GCGCGGGTGG GCGGTCGGGG 
GCGGGGGGCG ACGGTGAGGA CGACGGCCCC GTGTGGATCC CCAGCCCAGC CAGCCGGAGC 
TACCTGCTCA GCGTGCGGCC CGAGACCAGG AGCACCTTCT GCTCCATCAT TGCTCAGCTC 
ACAGAGGAGA CCCAGCCGCT ATTTGAGACC ACGCTCAAGT CCCGGTCTGT GTCCGAGGAC 
AGCGACGTCA GGTTCACCTG CATCGTCACA GGATACCCAG AGCCAGAGGT GACCTGGTAC 
AAGGATGATA CGGAGCTGGA CCGCTACTGT GGCTTGCCAA AATATGAGAT CACTCATCAG 
GGCAACCGCC ACACACTGCA GCTGTACAGG TGTCGAGAAG AAGATGCCGC CATCTACCAG 
GCCTCTGCCC AGAACAGCAA GGGCATTGTG TCCTGCTCAG GGGTCCTGGA GGTGGGCACC 
ATGACTGAGT ACAAGATCCA CCAGCGCTGG TTCGCCAAGT TGAAGCGCAA GGCTGCGGCA 
AAGCTGCGCG AGATCGAGCA GAGCTGGAAG CACGAGAAGG CGGTGCCTGG GGAGGTCGAC 
ACTCTGCGCA AGCTCAGCCC CGACCGCTTC CAGCGAAAGC GGCGATTGAG CGGGGCTCAA 
GCGCCGGGCC CCTCGGTCCC TACCAGGGAG CCTGAGGGTG GGACCCTGGC GGCTTGGCAG 
GAGGGAGAGA CTGAGACTGC TCAGCACTCA GGTTTGGGCC TGATCAACAG TTTTGCTTCT 
GGAGAAGTGA CCACCAACGG GGAGGCTGCC CCCGAGAATG GAGAGGACGG AGAGCATGGC 
TTGCTGACAT ACATCTGTGA CGCCATGGAG CTGGGGCCTC AGAGAGCCCT CAAAGAGGAG 
AGTGGGGCCA AGAAGAAAAA GAAAGATGAG GAATCCAAGC AAGGCCTGCG GAAGCCAGAG 
TTAGAGAAGG CAGCCCAAAG CCGCCGTTCT TCAGAAAACT GCATCCCCAG CTCAGACGAG 
CCTGACTCCT GTGGGACTCA GGGGCCCGTG GGCGTGGAGC AGGTTCAGAC CCAGCCCAGA 
GGCAGGGCTG CACGGGGGCC TGGGTCCTCT GGCACAGATA GTACCAGGAA GCCAGCCTCT 
GCTGTGGGCA CTCCAGACAA GGCCCAGAAG GCCCCTGGCC CAGGCCCAGG CCAGGAAGTG 
TATTTCTCCT TGAAGGACAT GTACCTGGAG AACACCCAGG CAGTCAGGCC TCTTGGGGAA 
GAGGGACCCC AGACCCTGAG TGTCCGGGCG CCTGGGGAGA GTCCCAAGGG GAAGGCACCC 
CTCAGGGCTA GAAGCGAGGG GGTGCCTGGC GCTCCTGGCC AGCCCACACA CTCCTTGACC 
CCCCAGCCGA CTAGGCCTTT CAACAGAAAG AGATTTGCCC CTCCAAAGCC CAAAGGAGAG 
GCCACCACTG ACAGCAAGCC CATTTCTTCT CTGAGTCAAG CTCCAGAATG CGGGGCCCAG 
AGCTTAGGAA AGGCCCCACC TCAGGCCTCT GTGCAGGTGC CGACGCCCCC TGCCCGGCGG 
AGACATGGCA CCCGGGACAG CACGTTGCAG GGGCAAGCAG GCCACAGGAC TCCAGGAGAG 
GTCCTGGAAT GCCAGACAAC CACGGCTCCT ACCATGTCGG CCAGCAGCAG CTCTGATGTA 
GCCTCCATTG GGGTTAGCAC TTCCGGAAGT CAAGGTATCA TTGAACCCAT GGATATGGAA 
ACCCAGGAGG ATGGGAGAAC ATCTGCTAAC CAGAGAACTG GAAGCAAGAA GAATGTGCAG 
GCAGATGGGA AGATACAAGT GGATGGAAGG ACCAGGGGAG ATGGAACACA GACAGCCCAG 
AGGACACGTG CAGATAGGAA GACGCAGGTG GATGCTGGGA CACAAGAAAG CAAGAGGCCA 
CAGTCAGACA GGAGTGCACA GAAGGGCATG ATGACACAGG GAAGGGCAGA GACACAGCTA 
GAAACAACAC AGGCAGGTGA GAAGATACAG GAAGACAGGA AGGCCCAGGC AGATAAGGGC 
ACACAGGAAG ACAGAAGGAT GCAGGGAGAG AAGGGGATGC AGGGAGAGAA GGGGACGCAG 
TCAGAGGGGA GCGCGCCCAC AGCCATGGAA GGTCAGTCTG AGCAAGAGGT GGCAACCAGC 
CTCGGCCCAC CATCCAGAAC CCCCAAACTC CCACCTACAG CGGGTCCTAG AGCTCCTCTG 
AATATTGAAT GTTTTGTACA GACCCCAGAA GGGTCTTGTT TCCCAAAAAA ACCTGGTTGC 
CTGCCCAGAT CTGAGGAGGC AGTAGTAACA GCCTCCAGGA ACCATGAGCA AACTGTGCTG 
GGTCCCCTGT CAGGGAACCT CATGCTCCCA GCACAGCCGC CCCATGAGGG GAGTGTGGAG 
CAGGTGGGAG GAGAGAGATG CCGAGGGCCA CAGTCATCAG GCCCAGTCGA GGCCAAGCAG 
GAGGACAGCC CGTTCCAGTG CCCCAAGGAG GAGCGGCCAG GGGGAGTGCC GTGTATGGAT 
CAGGGTGGCT GTCCTCTAGC TGGCCTGAGC CAGGAGGTAC CCACGATGCC TTCTCTTCCT 
GGAACTGGGC TGACAGCTAG CCCAAAGGCG GGGCCGTGTA GCACCCCGAC TTCTCAGCAC 
GGGAGCACAG CCACCTTCCT GCCCTCTGAG GATCAGGTCC TGATGAGTTC TGCCCCAACA 
CTGCACCTGG GGCTGGGGAC CCCCACTCAG AGTCACCCAC CAGAAACCAT GGCCACCAGC 
AGTGAGGGGG CCTGCGCCCA GGTACCAGAT GTGGAGGGGC GGACCCCAGG TCCCCGGAGC 
TGTGACCCTG GCCTCATAGA TTCCCTGAAG AACTACCTGC TTCTGCTGCT GAAGCTGTCC 
AGCACAGAGA CAAGTGGAGC AGGGGGAGAG TCCCAGGTGG GGGCAGCCAC CGGAGGTCTG 
GTGCCCTCAG CCACTCTGAC ACCCACTGTG GAAGTGGCTG GGCTTAGTCC CCGGACATCG 
AGGCGCATCC TGGAGCGTGT GGAGAACAAC CACCTGGTGC AGAGTGCACA GACCCTGCTG 
CTGAGCCCCT GTACCTCCCG CCGCCTCACC GGCCTCCTGG ACCGTGAGGT GCAGGCTGGC 
CGCCAGGCCC TTGCTGCTGC CCGAGGCTCC TGGGGTCCTG GTCCCAGCTC CCTCACTGTC 
CCTGCCATTG TGGTAGACGA GGAGGACCCT GGGCTGGCCT CAGAAGGAGC CAGTGAGGGT 
GAAGGAGAGG TTTCCCCTGA GGGGCCTGGC CTCCTGGGGG CCTCTCAGGA GAGCAGCATG 
GCTGGTCGAC TGGGGGAGGC GGGTGGGCAG GCAGCCCCTG GACAGGGGCC CTCAGCAGAG 
AGCATAGCCC AGGAGCCCTC CCAAGAGGAG AAGTTCCCAG GGGAGGCTCT GACAGGTCTC 
CCGGCAGCTA CACCTGAGGA ACTGGCTCTA GGGGCCCGGA GGAAGAGATT TCTCCCTAAG 
GTCAGAGCAG CAGGAGACGG GGAGGCAACC ACACCTGAAG AAAGGGAGAG CCCCACGGTT 
TCCCCCCGGG GGCCCAGGAA AAGCCTGGTG CCTGGGTCCC CAGGGACTCC AGGGCGGGAG 
AGACGCTCCC CTACGCAGGG CAGAAAGGCG AGCATGCTGG AGGTGCCTCG GGCAGAGGAG 
GAGCTGGCGG CAGGAGACCT GGGCCCCAGC CCCAAGGCCG GCGGTCTGGA CACAGAGGTG 
GCCCTGGATG AAGGCAAGCA GGAGACACTG GCCAAGCCCA GGAAAGCCAA AGACCTGCTG 
AAAGCCCCAC AGGTGATCCG GAAGATTCGG GTGGAGCAGT TTCCTGATGC CTCCGGTAGC 
CTGAAGCTGT GGTGCCAGTT TTTCAACATT CTTAGTGACT CAGTCTTGAC ATGGGCCAAG 
GATCAGCGCC CAGTGGGCGA GGTGGGCAGG AGCGCAGGGG ATGAGGGGCC GGCGGCCTTG 
GCCATCGTGC AGGCCTCCCC CGTAGACTGC GGTGTGTATC GGTGCACCAT CCACAATGAG 
CACGGCTCGG CCTCCACCGA CTTCTGCCTC AGCCCTGAGG TGTTGTCAGG ATTCATCTCC 
AGAGAAGAAG GTGAAGTTGG AGAAGAGATT GAGATGACCC CTATGGTGTT TGCTAAGGGT 
CTGGCTGACT CTGGCTGCTG GGGGGACAAG CTCTTTGGGC GACTGGTAAG CGAGGAGCTC 
CGAGGGGGTG GATATGGGTG TGGCCTTCGG AAGGCCTCCC AGGCCAAGGT CATCTACGGG 
CTGGAACCCA TCTTCGAGTC GGGCCGCACG TGCATCATCA AGGTGTCCAG CCTGCTTGTG 
TTTGGGCCCA GCAGTGAGAC TTCTCTTGTG GGCAGAAACT ACGACGTCAC CATCCAGGGG 
TGCAAGATCC AGAACATGAG TCGGGAGTAC TGCAAAATCT TCGCAGCAGA AGCCCGGGCC 
GCGCCTGGCT TTGGGGAGGT GCCTGAGATC ATCCCACTGT ATCTGATCTA CCGGCCTGCA 
AACAATATCC CATATGCTAC CCTGGAGGAA GACCTGGGCA AGCCCCTGGA GTCTTACTGT 
TCTCGGGAAT GGGGCTGTGC TGAGGCTCCG ACAGCATCTG GCAGCTCTGA GGCCATGCAG 
AAATGCCAGA CCTTCCAACA CTGGCTGTAT CAGTGGACAA ATGGCAGCTT CCTTGTCACA 
GACTTGGCAG GGGTTGACTG GAAGATGACT GATGTGCAGA TTGCTACCAA ACTCCGAGGA 
TACCAGGGCC TCAAGGAAAG CTGCTTCCCT GCCCTGCTGG ACCGGTTCGC CTCCTCCCAC 
CAGTGCAATG CCTACTGTGA GCTGCTGGGG CTGACACCTC TCAAGGGCCC GGAGGCGGCC 
CACCCCCAAG CCAAAGCCAA AGGCTCTAAG AGTCCATCTG CTGGCAGGAA AGGCTCCCAG 
CTGAGTCCTC AGCCCCAGAA GAAAGGCCTC CCTAGTCCTC AGGGCACCCG GAAGAGTGCT 
CCAAGTTCCA AGGCCACCCC TCAGGCCTCA GAGCCAGTCA CCACTCAGTT GTTGGGACAG 
CCTCCCACCC AAGAGGAGGG CTCCAAGGCC CAGGGCATGC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877933.1
CDS	205..5889
Translation	MEVAWLVYVLGQQPLARQGEGQSRLVPGRGLVLWLPGLPRSSPSWPAVDLAPLAPARPRGPLICHTGHEQAGREPGPGSSTKGPVLHDQDTRCAFLPRPPGPLQTRRYCRHQGRQGSGLGAGPGAGTWAPAPPGVSKPRCPGRARPGEGQQQVTTARPPAINRGARQPRAGAAAAGRGPGAGAWRTGEAAASAGPAVGEGGAMGSRRAPSRGWGAGGRSGAGGDGEDDGPVWIPSPASRSYLLSVRPETRSTFCSIIAQLTEETQPLFETTLKSRSVSEDSDVRFTCIVTGYPEPEVTWYKDDTELDRYCGLPKYEITHQGNRHTLQLYRCREEDAAIYQASAQNSKGIVSCSGVLEVGTMTEYKIHQRWFAKLKRKAAAKLREIEQSWKHEKAVPGEVDTLRKLSPDRFQRKRRLSGAQAPGPSVPTREPEGGTLAAWQEGETETAQHSGLGLINSFASGEVTTNGEAAPENGEDGEHGLLTYICDAMELGPQRALKEESGAKKKKKDEESKQGLRKPELEKAAQSRRSSENCIPSSDEPDSCGTQGPVGVEQVQTQPRGRAARGPGSSGTDSTRKPASAVGTPDKAQKAPGPGPGQEVYFSLKDMYLENTQAVRPLGEEGPQTLSVRAPGESPKGKAPLRARSEGVPGAPGQPTHSLTPQPTRPFNRKRFAPPKPKGEATTDSKPISSLSQAPECGAQSLGKAPPQASVQVPTPPARRRHGTRDSTLQGQAGHRTPGEVLECQTTTAPTMSASSSSDVASIGVSTSGSQGIIEPMDMETQEDGRTSANQRTGSKKNVQADGKIQVDGRTRGDGTQTAQRTRADRKTQVDAGTQESKRPQSDRSAQKGMMTQGRAETQLETTQAGEKIQEDRKAQADKGTQEDRRMQGEKGMQGEKGTQSEGSAPTAMEGQSEQEVATSLGPPSRTPKLPPTAGPRAPLNIECFVQTPEGSCFPKKPGCLPRSEEAVVTASRNHEQTVLGPLSGNLMLPAQPPHEGSVEQVGGERCRGPQSSGPVEAKQEDSPFQCPKEERPGGVPCMDQGGCPLAGLSQEVPTMPSLPGTGLTASPKAGPCSTPTSQHGSTATFLPSEDQVLMSSAPTLHLGLGTPTQSHPPETMATSSEGACAQVPDVEGRTPGPRSCDPGLIDSLKNYLLLLLKLSSTETSGAGGESQVGAATGGLVPSATLTPTVEVAGLSPRTSRRILERVENNHLVQSAQTLLLSPCTSRRLTGLLDREVQAGRQALAAARGSWGPGPSSLTVPAIVVDEEDPGLASEGASEGEGEVSPEGPGLLGASQESSMAGRLGEAGGQAAPGQGPSAESIAQEPSQEEKFPGEALTGLPAATPEELALGARRKRFLPKVRAAGDGEATTPEERESPTVSPRGPRKSLVPGSPGTPGRERRSPTQGRKASMLEVPRAEEELAAGDLGPSPKAGGLDTEVALDEGKQETLAKPRKAKDLLKAPQVIRKIRVEQFPDASGSLKLWCQFFNILSDSVLTWAKDQRPVGEVGRSAGDEGPAALAIVQASPVDCGVYRCTIHNEHGSASTDFCLSPEVLSGFISREEGEVGEEIEMTPMVFAKGLADSGCWGDKLFGRLVSEELRGGGYGCGLRKASQAKVIYGLEPIFESGRTCIIKVSSLLVFGPSSETSLVGRNYDVTIQGCKIQNMSREYCKIFAAEARAAPGFGEVPEIIPLYLIYRPANNIPYATLEEDLGKPLESYCSREWGCAEAPTASGSSEAMQKCQTFQHWLYQWTNGSFLVTDLAGVDWKMTDVQIATKLRGYQGLKESCFPALLDRFASSHQCNAYCELLGLTPLKGPEAAHPQAKAKGSKSPSAGRKGSQLSPQPQKKGLPSPQGTRKSAPSSKATPQASEPVTTQLLGQPPTQEEGSKAQGMR

Target ORF information:

RefSeq Version	XM_017022444.1
Organism	Homo sapiens(human)
Definition	Homo sapiens alpha kinase 3 (ALPK3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022444.1

ORF Insert Sequence:

ATGGAGGTGG CTTGGCTTGT CTATGTGCTG GGCCAACAGC CACTGGCGAG GCAAGGCGAG 
GGTCAGTCAC GGCTGGTGCC AGGAAGAGGG CTGGTTCTTT GGCTCCCTGG TCTCCCGCGG 
TCTAGCCCAA GCTGGCCAGC GGTTGACCTG GCTCCCCTGG CCCCGGCCAG GCCTCGTGGA 
CCCCTCATAT GCCACACGGG ACATGAGCAG GCCGGCCGGG AGCCGGGTCC CGGGAGCTCC 
ACGAAGGGGC CTGTCCTCCA TGACCAGGAC ACCCGCTGCG CCTTCCTCCC GAGGCCTCCC 
GGGCCTCTCC AGACGCGGCG CTACTGCAGA CACCAGGGCC GCCAAGGGAG CGGACTCGGA 
GCCGGCCCTG GGGCGGGCAC ATGGGCCCCG GCGCCCCCCG GCGTCTCCAA GCCGCGCTGC 
CCGGGTCGGG CCAGGCCAGG GGAGGGACAG CAGCAGGTGA CGACGGCCCG GCCACCGGCT 
ATAAATAGGG GCGCGCGTCA GCCGCGGGCG GGAGCGGCGG CGGCGGGCAG GGGCCCGGGG 
GCCGGGGCCT GGAGGACAGG CGAGGCAGCG GCGAGTGCGG GGCCGGCGGT CGGGGAGGGC 
GGTGCCATGG GGTCGCGGAG GGCCCCCAGC CGGGGCTGGG GCGCGGGTGG GCGGTCGGGG 
GCGGGGGGCG ACGGTGAGGA CGACGGCCCC GTGTGGATCC CCAGCCCAGC CAGCCGGAGC 
TACCTGCTCA GCGTGCGGCC CGAGACCAGG AGCACCTTCT GCTCCATCAT TGCTCAGCTC 
ACAGAGGAGA CCCAGCCGCT ATTTGAGACC ACGCTCAAGT CCCGGTCTGT GTCCGAGGAC 
AGCGACGTCA GGTTCACCTG CATCGTCACA GGATACCCAG AGCCAGAGGT GACCTGGTAC 
AAGGATGATA CGGAGCTGGA CCGCTACTGT GGCTTGCCAA AATATGAGAT CACTCATCAG 
GGCAACCGCC ACACACTGCA GCTGTACAGG TGTCGAGAAG AAGATGCCGC CATCTACCAG 
GCCTCTGCCC AGAACAGCAA GGGCATTGTG TCCTGCTCAG GGGTCCTGGA GGTGGGCACC 
ATGACTGAGT ACAAGATCCA CCAGCGCTGG TTCGCCAAGT TGAAGCGCAA GGCTGCGGCA 
AAGCTGCGCG AGATCGAGCA GAGCTGGAAG CACGAGAAGG CGGTGCCTGG GGAGGTCGAC 
ACTCTGCGCA AGCTCAGCCC CGACCGCTTC CAGCGAAAGC GGCGATTGAG CGGGGCTCAA 
GCGCCGGGCC CCTCGGTCCC TACCAGGGAG CCTGAGGGTG GGACCCTGGC GGCTTGGCAG 
GAGGGAGAGA CTGAGACTGC TCAGCACTCA GGTTTGGGCC TGATCAACAG TTTTGCTTCT 
GGAGAAGTGA CCACCAACGG GGAGGCTGCC CCCGAGAATG GAGAGGACGG AGAGCATGGC 
TTGCTGACAT ACATCTGTGA CGCCATGGAG CTGGGGCCTC AGAGAGCCCT CAAAGAGGAG 
AGTGGGGCCA AGAAGAAAAA GAAAGATGAG GAATCCAAGC AAGGCCTGCG GAAGCCAGAG 
TTAGAGAAGG CAGCCCAAAG CCGCCGTTCT TCAGAAAACT GCATCCCCAG CTCAGACGAG 
CCTGACTCCT GTGGGACTCA GGGGCCCGTG GGCGTGGAGC AGGTTCAGAC CCAGCCCAGA 
GGCAGGGCTG CACGGGGGCC TGGGTCCTCT GGCACAGATA GTACCAGGAA GCCAGCCTCT 
GCTGTGGGCA CTCCAGACAA GGCCCAGAAG GCCCCTGGCC CAGGCCCAGG CCAGGAAGTG 
TATTTCTCCT TGAAGGACAT GTACCTGGAG AACACCCAGG CAGTCAGGCC TCTTGGGGAA 
GAGGGACCCC AGACCCTGAG TGTCCGGGCG CCTGGGGAGA GTCCCAAGGG GAAGGCACCC 
CTCAGGGCTA GAAGCGAGGG GGTGCCTGGC GCTCCTGGCC AGCCCACACA CTCCTTGACC 
CCCCAGCCGA CTAGGCCTTT CAACAGAAAG AGATTTGCCC CTCCAAAGCC CAAAGGAGAG 
GCCACCACTG ACAGCAAGCC CATTTCTTCT CTGAGTCAAG CTCCAGAATG CGGGGCCCAG 
AGCTTAGGAA AGGCCCCACC TCAGGCCTCT GTGCAGGTGC CGACGCCCCC TGCCCGGCGG 
AGACATGGCA CCCGGGACAG CACGTTGCAG GGGCAAGCAG GCCACAGGAC TCCAGGAGAG 
GTCCTGGAAT GCCAGACAAC CACGGCTCCT ACCATGTCGG CCAGCAGCAG CTCTGATGTA 
GCCTCCATTG GGGTTAGCAC TTCCGGAAGT CAAGGTATCA TTGAACCCAT GGATATGGAA 
ACCCAGGAGG ATGGGAGAAC ATCTGCTAAC CAGAGAACTG GAAGCAAGAA GAATGTGCAG 
GCAGATGGGA AGATACAAGT GGATGGAAGG ACCAGGGGAG ATGGAACACA GACAGCCCAG 
AGGACACGTG CAGATAGGAA GACGCAGGTG GATGCTGGGA CACAAGAAAG CAAGAGGCCA 
CAGTCAGACA GGAGTGCACA GAAGGGCATG ATGACACAGG GAAGGGCAGA GACACAGCTA 
GAAACAACAC AGGCAGGTGA GAAGATACAG GAAGACAGGA AGGCCCAGGC AGATAAGGGC 
ACACAGGAAG ACAGAAGGAT GCAGGGAGAG AAGGGGATGC AGGGAGAGAA GGGGACGCAG 
TCAGAGGGGA GCGCGCCCAC AGCCATGGAA GGTCAGTCTG AGCAAGAGGT GGCAACCAGC 
CTCGGCCCAC CATCCAGAAC CCCCAAACTC CCACCTACAG CGGGTCCTAG AGCTCCTCTG 
AATATTGAAT GTTTTGTACA GACCCCAGAA GGGTCTTGTT TCCCAAAAAA ACCTGGTTGC 
CTGCCCAGAT CTGAGGAGGC AGTAGTAACA GCCTCCAGGA ACCATGAGCA AACTGTGCTG 
GGTCCCCTGT CAGGGAACCT CATGCTCCCA GCACAGCCGC CCCATGAGGG GAGTGTGGAG 
CAGGTGGGAG GAGAGAGATG CCGAGGGCCA CAGTCATCAG GCCCAGTCGA GGCCAAGCAG 
GAGGACAGCC CGTTCCAGTG CCCCAAGGAG GAGCGGCCAG GGGGAGTGCC GTGTATGGAT 
CAGGGTGGCT GTCCTCTAGC TGGCCTGAGC CAGGAGGTAC CCACGATGCC TTCTCTTCCT 
GGAACTGGGC TGACAGCTAG CCCAAAGGCG GGGCCGTGTA GCACCCCGAC TTCTCAGCAC 
GGGAGCACAG CCACCTTCCT GCCCTCTGAG GATCAGGTCC TGATGAGTTC TGCCCCAACA 
CTGCACCTGG GGCTGGGGAC CCCCACTCAG AGTCACCCAC CAGAAACCAT GGCCACCAGC 
AGTGAGGGGG CCTGCGCCCA GGTACCAGAT GTGGAGGGGC GGACCCCAGG TCCCCGGAGC 
TGTGACCCTG GCCTCATAGA TTCCCTGAAG AACTACCTGC TTCTGCTGCT GAAGCTGTCC 
AGCACAGAGA CAAGTGGAGC AGGGGGAGAG TCCCAGGTGG GGGCAGCCAC CGGAGGTCTG 
GTGCCCTCAG CCACTCTGAC ACCCACTGTG GAAGTGGCTG GGCTTAGTCC CCGGACATCG 
AGGCGCATCC TGGAGCGTGT GGAGAACAAC CACCTGGTGC AGAGTGCACA GACCCTGCTG 
CTGAGCCCCT GTACCTCCCG CCGCCTCACC GGCCTCCTGG ACCGTGAGGT GCAGGCTGGC 
CGCCAGGCCC TTGCTGCTGC CCGAGGCTCC TGGGGTCCTG GTCCCAGCTC CCTCACTGTC 
CCTGCCATTG TGGTAGACGA GGAGGACCCT GGGCTGGCCT CAGAAGGAGC CAGTGAGGGT 
GAAGGAGAGG TTTCCCCTGA GGGGCCTGGC CTCCTGGGGG CCTCTCAGGA GAGCAGCATG 
GCTGGTCGAC TGGGGGAGGC GGGTGGGCAG GCAGCCCCTG GACAGGGGCC CTCAGCAGAG 
AGCATAGCCC AGGAGCCCTC CCAAGAGGAG AAGTTCCCAG GGGAGGCTCT GACAGGTCTC 
CCGGCAGCTA CACCTGAGGA ACTGGCTCTA GGGGCCCGGA GGAAGAGATT TCTCCCTAAG 
GTCAGAGCAG CAGGAGACGG GGAGGCAACC ACACCTGAAG AAAGGGAGAG CCCCACGGTT 
TCCCCCCGGG GGCCCAGGAA AAGCCTGGTG CCTGGGTCCC CAGGGACTCC AGGGCGGGAG 
AGACGCTCCC CTACGCAGGG CAGAAAGGCG AGCATGCTGG AGGTGCCTCG GGCAGAGGAG 
GAGCTGGCGG CAGGAGACCT GGGCCCCAGC CCCAAGGCCG GCGGTCTGGA CACAGAGGTG 
GCCCTGGATG AAGGCAAGCA GGAGACACTG GCCAAGCCCA GGAAAGCCAA AGACCTGCTG 
AAAGCCCCAC AGGTGATCCG GAAGATTCGG GTGGAGCAGT TTCCTGATGC CTCCGGTAGC 
CTGAAGCTGT GGTGCCAGTT TTTCAACATT CTTAGTGACT CAGTCTTGAC ATGGGCCAAG 
GATCAGCGCC CAGTGGGCGA GGTGGGCAGG AGCGCAGGGG ATGAGGGGCC GGCGGCCTTG 
GCCATCGTGC AGGCCTCCCC CGTAGACTGC GGTGTGTATC GGTGCACCAT CCACAATGAG 
CACGGCTCGG CCTCCACCGA CTTCTGCCTC AGCCCTGAGG TGTTGTCAGG ATTCATCTCC 
AGAGAAGAAG GTGAAGTTGG AGAAGAGATT GAGATGACCC CTATGGTGTT TGCTAAGGGT 
CTGGCTGACT CTGGCTGCTG GGGGGACAAG CTCTTTGGGC GACTGGTAAG CGAGGAGCTC 
CGAGGGGGTG GATATGGGTG TGGCCTTCGG AAGGCCTCCC AGGCCAAGGT CATCTACGGG 
CTGGAACCCA TCTTCGAGTC GGGCCGCACG TGCATCATCA AGGTGTCCAG CCTGCTTGTG 
TTTGGGCCCA GCAGTGAGAC TTCTCTTGTG GGCAGAAACT ACGACGTCAC CATCCAGGGG 
TGCAAGATCC AGAACATGAG TCGGGAGTAC TGCAAAATCT TCGCAGCAGA AGCCCGGGCC 
GCGCCTGGCT TTGGGGAGGT GCCTGAGATC ATCCCACTGT ATCTGATCTA CCGGCCTGCA 
AACAATATCC CATATGCTAC CCTGGAGGAA GACCTGGGCA AGCCCCTGGA GTCTTACTGT 
TCTCGGGAAT GGGGCTGTGC TGAGGCTCCG ACAGCATCTG GCAGCTCTGA GGCCATGCAG 
AAATGCCAGA CCTTCCAACA CTGGCTGTAT CAGTGGACAA ATGGCAGCTT CCTTGTCACA 
GACTTGGCAG GGGTTGACTG GAAGATGACT GATGTGCAGA TTGCTACCAA ACTCCGAGGA 
TACCAGGGCC TCAAGGAAAG CTGCTTCCCT GCCCTGCTGG ACCGGTTCGC CTCCTCCCAC 
CAGTGCAATG CCTACTGTGA GCTGCTGGGG CTGACACCTC TCAAGGGCCC GGAGGCGGCC 
CACCCCCAAG CCAAAGCCAA AGGCTCTAAG AGTCCATCTG CTGGCAGGAA AGGCTCCCAG 
CTGAGTCCTC AGCCCCAGAA GAAAGGCCTC CCTAGTCCTC AGGGCACCCG GAAGAGTGCT 
CCAAGTTCCA AGGCCACCCC TCAGGCCTCA GAGCCAGTCA CCACTCAGTT GTTGGGACAG 
CCTCCCACCC AAGAGGAGGG CTCCAAGGCC CAGGGCATGC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALPK3 ( XM_017022445.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877934.1 Homo sapiens alpha kinase 3 (ALPK3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu85577
Clone ID Related Accession (Same CDS sequence)	XM_017022445.1
Accession Version	XM_017022445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	alpha-protein kinase 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGG CTTGGCTTGT CTATGTGCTG GGCCAACAGC CACTGGCGAG GCAAGGCGAG 
GGTCAGTCAC GGCTGGTGCC AGGAAGAGGG CTGGTTCTTT GGCTCCCTGG TCTCCCGCGG 
TCTAGCCCAA GCTGGCCAGC GGTTGACCTG GCTCCCCTGG CCCCGGCCAG GCCTCGTGGA 
CCCCTCATAT GCCACACGGG ACATGAGCAG GCCGGCCGGG AGCCGGGTCC CGGGAGCTCC 
ACGAAGGGGC CTGTCCTCCA TGACCAGGAC ACCCGCTGCG CCTTCCTCCC GAGGCCTCCC 
GGGCCTCTCC AGACGCGGCG CTACTGCAGA CACCAGGGCC GCCAAGGGAG CGGACTCGGA 
GCCGGCCCTG GGGCGGGCAC ATGGGCCCCG GCGCCCCCCG GCGTCTCCAA GCCGCGCTGC 
CCGGGTCGGG CCAGGCCAGG GGAGGGACAG CAGCAGGTGA CGACGGCCCG GCCACCGGCT 
ATAAATAGGG GCGCGCGTCA GCCGCGGGCG GGAGCGGCGG CGGCGGGCAG GGGCCCGGGG 
GCCGGGGCCT GGAGGACAGG CGAGGCAGCG GCGAGTGCGG GGCCGGCGGT CGGGGAGGGC 
GGTGCCATGG GGTCGCGGAG GGCCCCCAGC CGGGGCTGGG GCGCGGGTGG GCGGTCGGGG 
GCGGGGGGCG ACGGTGAGGA CGACGGCCCC GTGTGGATCC CCAGCCCAGC CAGCCGGAGC 
TACCTGCTCA GCGTGCGGCC CGAGACCAGC TTATCAAGCA ACCGGTTGTC TCACCCCAGC 
TCTGGAAGGA GCACCTTCTG CTCCATCATT GCTCAGCTCA CAGAGGAGAC CCAGCCGCTA 
TTTGAGACCA CGCTCAAGTC CCGGTCTGTG TCCGAGGACA GCGACGTCAG GTTCACCTGC 
ATCGTCACAG GATACCCAGA GCCAGAGGTG ACCTGGTACA AGGATGATAC GGAGCTGGAC 
CGCTACTGTG GCTTGCCAAA ATATGAGATC ACTCATCAGG GCAACCGCCA CACACTGCAG 
CTGTACAGGT GTCGAGAAGA AGATGCCGCC ATCTACCAGG CCTCTGCCCA GAACAGCAAG 
GGCATTGTGT CCTGCTCAGG GGTCCTGGAG GTGGGCACCA TGACTGAGTA CAAGATCCAC 
CAGCGCTGGT TCGCCAAGTT GAAGCGCAAG GCTGCGGCAA AGCTGCGCGA GATCGAGCAG 
AGCTGGAAGC ACGAGAAGGC GGTGCCTGGG GAGGTCGACA CTCTGCGCAA GCTCAGCCCC 
GACCGCTTCC AGCGAAAGCG GCGATTGAGC GGGGCTCAAG CGCCGGGCCC CTCGGTCCCT 
ACCAGGGAGC CTGAGGGTGG GACCCTGGCG GCTTGGCAGG AGGGAGAGAC TGAGACTGCT 
CAGCACTCAG GTTTGGGCCT GATCAACAGT TTTGCTTCTG GAGAAGTGAC CACCAACGGG 
GAGGCTGCCC CCGAGAATGG AGAGGACGGA GAGCATGGCT TGCTGACATA CATCTGTGAC 
GCCATGGAGC TGGGGCCTCA GAGAGCCCTC AAAGAGGAGA GTGGGGCCAA GAAGAAAAAG 
AAAGATGAGG AATCCAAGCA AGGCCTGCGG AAGCCAGAGT TAGAGAAGGC AGCCCAAAGC 
CGCCGTTCTT CAGAAAACTG CATCCCCAGC TCAGACGAGC CTGACTCCTG TGGGACTCAG 
GGGCCCGTGG GCGTGGAGCA GGTTCAGACC CAGCCCAGAG GCAGGGCTGC ACGGGGGCCT 
GGGTCCTCTG GCACAGATAG TACCAGGAAG CCAGCCTCTG CTGTGGGCAC TCCAGACAAG 
GCCCAGAAGG CCCCTGGCCC AGGCCCAGGC CAGGAAGTGT ATTTCTCCTT GAAGGACATG 
TACCTGGAGA ACACCCAGGC AGTCAGGCCT CTTGGGGAAG AGGGACCCCA GACCCTGAGT 
GTCCGGGCGC CTGGGGAGAG TCCCAAGGGG AAGGCACCCC TCAGGGCTAG AAGCGAGGGG 
GTGCCTGGCG CTCCTGGCCA GCCCACACAC TCCTTGACCC CCCAGCCGAC TAGGCCTTTC 
AACAGAAAGA GATTTGCCCC TCCAAAGCCC AAAGGAGAGG CCACCACTGA CAGCAAGCCC 
ATTTCTTCTC TGAGTCAAGC TCCAGAATGC GGGGCCCAGA GCTTAGGAAA GGCCCCACCT 
CAGGCCTCTG TGCAGGTGCC GACGCCCCCT GCCCGGCGGA GACATGGCAC CCGGGACAGC 
ACGTTGCAGG GGCAAGCAGG CCACAGGACT CCAGGAGAGG TCCTGGAATG CCAGACAACC 
ACGGCTCCTA CCATGTCGGC CAGCAGCAGC TCTGATGTAG CCTCCATTGG GGTTAGCACT 
TCCGGAAGTC AAGGTATCAT TGAACCCATG GATATGGAAA CCCAGGAGGA TGGGAGAACA 
TCTGCTAACC AGAGAACTGG AAGCAAGAAG AATGTGCAGG CAGATGGGAA GATACAAGTG 
GATGGAAGGA CCAGGGGAGA TGGAACACAG ACAGCCCAGA GGACACGTGC AGATAGGAAG 
ACGCAGGTGG ATGCTGGGAC ACAAGAAAGC AAGAGGCCAC AGTCAGACAG GAGTGCACAG 
AAGGGCATGA TGACACAGGG AAGGGCAGAG ACACAGCTAG AAACAACACA GGCAGGTGAG 
AAGATACAGG AAGACAGGAA GGCCCAGGCA GATAAGGGCA CACAGGAAGA CAGAAGGATG 
CAGGGAGAGA AGGGGATGCA GGGAGAGAAG GGGACGCAGT CAGAGGGGAG CGCGCCCACA 
GCCATGGAAG GTCAGTCTGA GCAAGAGGTG GCAACCAGCC TCGGCCCACC ATCCAGAACC 
CCCAAACTCC CACCTACAGC GGGTCCTAGA GCTCCTCTGA ATATTGAATG TTTTGTACAG 
ACCCCAGAAG GGTCTTGTTT CCCAAAAAAA CCTGGTTGCC TGCCCAGATC TGAGGAGGCA 
GTAGTAACAG CCTCCAGGAA CCATGAGCAA ACTGTGCTGG GTCCCCTGTC AGGGAACCTC 
ATGCTCCCAG CACAGCCGCC CCATGAGGGG AGTGTGGAGC AGGTGGGAGG AGAGAGATGC 
CGAGGGCCAC AGTCATCAGG CCCAGTCGAG GCCAAGCAGG AGGACAGCCC GTTCCAGTGC 
CCCAAGGAGG AGCGGCCAGG GGGAGTGCCG TGTATGGATC AGGGTGGCTG TCCTCTAGCT 
GGCCTGAGCC AGGAGGTACC CACGATGCCT TCTCTTCCTG GAACTGGGCT GACAGCTAGC 
CCAAAGGCGG GGCCGTGTAG CACCCCGACT TCTCAGCACG GGAGCACAGC CACCTTCCTG 
CCCTCTGAGG ATCAGGTCCT GATGAGTTCT GCCCCAACAC TGCACCTGGG GCTGGGGACC 
CCCACTCAGA GTCACCCACC AGAAACCATG GCCACCAGCA GTGAGGGGGC CTGCGCCCAG 
GTACCAGATG TGGAGGGGCG GACCCCAGGT CCCCGGAGCT GTGACCCTGG CCTCATAGAT 
TCCCTGAAGA ACTACCTGCT TCTGCTGCTG AAGCTGTCCA GCACAGAGAC AAGTGGAGCA 
GGGGGAGAGT CCCAGGTGGG GGCAGCCACC GGAGGTCTGG TGCCCTCAGC CACTCTGACA 
CCCACTGTGG AAGTGGCTGG GCTTAGTCCC CGGACATCGA GGCGCATCCT GGAGCGTGTG 
GAGAACAACC ACCTGGTGCA GAGTGCACAG ACCCTGCTGC TGAGCCCCTG TACCTCCCGC 
CGCCTCACCG GCCTCCTGGA CCGTGAGGTG CAGGCTGGCC GCCAGGCCCT TGCTGCTGCC 
CGAGGCTCCT GGGGTCCTGG TCCCAGCTCC CTCACTGTCC CTGCCATTGT GGTAGACGAG 
GAGGACCCTG GGCTGGCCTC AGAAGGAGCC AGTGAGGGTG AAGGAGAGGT TTCCCCTGAG 
GGGCCTGGCC TCCTGGGGGC CTCTCAGGAG AGCAGCATGG CTGGTCGACT GGGGGAGGCG 
GGTGGGCAGG CAGCCCCTGG ACAGGGGCCC TCAGCAGAGA GCATAGCCCA GGAGCCCTCC 
CAAGAGGAGA AGTTCCCAGG GGAGGCTCTG ACAGGTCTCC CGGCAGCTAC ACCTGAGGAA 
CTGGCTCTAG GGGCCCGGAG GAAGAGATTT CTCCCTAAGG TCAGAGCAGC AGGAGACGGG 
GAGGCAACCA CACCTGAAGA AAGGGAGAGC CCCACGGTTT CCCCCCGGGG GCCCAGGAAA 
AGCCTGGTGC CTGGGTCCCC AGGGACTCCA GGGCGGGAGA GACGCTCCCC TACGCAGGGC 
AGAAAGGCGA GCATGCTGGA GGTGCCTCGG GCAGAGGAGG AGCTGGCGGC AGGAGACCTG 
GGCCCCAGCC CCAAGGCCGG CGGTCTGGAC ACAGAGGTGG CCCTGGATGA AGGCAAGCAG 
GAGACACTGG CCAAGCCCAG GAAAGCCAAA GACCTGCTGA AAGCCCCACA GGTGATCCGG 
AAGATTCGGG TGGAGCAGTT TCCTGATGCC TCCGGTAGCC TGAAGCTGTG GTGCCAGTTT 
TTCAACATTC TTAGTGACTC AGTCTTGACA TGGGCCAAGG ATCAGCGCCC AGTGGGCGAG 
GTGGGCAGGA GCGCAGGGGA TGAGGGGCCG GCGGCCTTGG CCATCGTGCA GGCCTCCCCC 
GTAGACTGCG GTGTGTATCG GTGCACCATC CACAATGAGC ACGGCTCGGC CTCCACCGAC 
TTCTGCCTCA GCCCTGAGGT GTTGTCAGGA TTCATCTCCA GAGAAGAAGG TGAAGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877934.1
CDS	217..4956
Translation	MEVAWLVYVLGQQPLARQGEGQSRLVPGRGLVLWLPGLPRSSPSWPAVDLAPLAPARPRGPLICHTGHEQAGREPGPGSSTKGPVLHDQDTRCAFLPRPPGPLQTRRYCRHQGRQGSGLGAGPGAGTWAPAPPGVSKPRCPGRARPGEGQQQVTTARPPAINRGARQPRAGAAAAGRGPGAGAWRTGEAAASAGPAVGEGGAMGSRRAPSRGWGAGGRSGAGGDGEDDGPVWIPSPASRSYLLSVRPETSLSSNRLSHPSSGRSTFCSIIAQLTEETQPLFETTLKSRSVSEDSDVRFTCIVTGYPEPEVTWYKDDTELDRYCGLPKYEITHQGNRHTLQLYRCREEDAAIYQASAQNSKGIVSCSGVLEVGTMTEYKIHQRWFAKLKRKAAAKLREIEQSWKHEKAVPGEVDTLRKLSPDRFQRKRRLSGAQAPGPSVPTREPEGGTLAAWQEGETETAQHSGLGLINSFASGEVTTNGEAAPENGEDGEHGLLTYICDAMELGPQRALKEESGAKKKKKDEESKQGLRKPELEKAAQSRRSSENCIPSSDEPDSCGTQGPVGVEQVQTQPRGRAARGPGSSGTDSTRKPASAVGTPDKAQKAPGPGPGQEVYFSLKDMYLENTQAVRPLGEEGPQTLSVRAPGESPKGKAPLRARSEGVPGAPGQPTHSLTPQPTRPFNRKRFAPPKPKGEATTDSKPISSLSQAPECGAQSLGKAPPQASVQVPTPPARRRHGTRDSTLQGQAGHRTPGEVLECQTTTAPTMSASSSSDVASIGVSTSGSQGIIEPMDMETQEDGRTSANQRTGSKKNVQADGKIQVDGRTRGDGTQTAQRTRADRKTQVDAGTQESKRPQSDRSAQKGMMTQGRAETQLETTQAGEKIQEDRKAQADKGTQEDRRMQGEKGMQGEKGTQSEGSAPTAMEGQSEQEVATSLGPPSRTPKLPPTAGPRAPLNIECFVQTPEGSCFPKKPGCLPRSEEAVVTASRNHEQTVLGPLSGNLMLPAQPPHEGSVEQVGGERCRGPQSSGPVEAKQEDSPFQCPKEERPGGVPCMDQGGCPLAGLSQEVPTMPSLPGTGLTASPKAGPCSTPTSQHGSTATFLPSEDQVLMSSAPTLHLGLGTPTQSHPPETMATSSEGACAQVPDVEGRTPGPRSCDPGLIDSLKNYLLLLLKLSSTETSGAGGESQVGAATGGLVPSATLTPTVEVAGLSPRTSRRILERVENNHLVQSAQTLLLSPCTSRRLTGLLDREVQAGRQALAAARGSWGPGPSSLTVPAIVVDEEDPGLASEGASEGEGEVSPEGPGLLGASQESSMAGRLGEAGGQAAPGQGPSAESIAQEPSQEEKFPGEALTGLPAATPEELALGARRKRFLPKVRAAGDGEATTPEERESPTVSPRGPRKSLVPGSPGTPGRERRSPTQGRKASMLEVPRAEEELAAGDLGPSPKAGGLDTEVALDEGKQETLAKPRKAKDLLKAPQVIRKIRVEQFPDASGSLKLWCQFFNILSDSVLTWAKDQRPVGEVGRSAGDEGPAALAIVQASPVDCGVYRCTIHNEHGSASTDFCLSPEVLSGFISREEGEV

Target ORF information:

RefSeq Version	XM_017022445.1
Organism	Homo sapiens(human)
Definition	Homo sapiens alpha kinase 3 (ALPK3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022445.1

ORF Insert Sequence:

ATGGAGGTGG CTTGGCTTGT CTATGTGCTG GGCCAACAGC CACTGGCGAG GCAAGGCGAG 
GGTCAGTCAC GGCTGGTGCC AGGAAGAGGG CTGGTTCTTT GGCTCCCTGG TCTCCCGCGG 
TCTAGCCCAA GCTGGCCAGC GGTTGACCTG GCTCCCCTGG CCCCGGCCAG GCCTCGTGGA 
CCCCTCATAT GCCACACGGG ACATGAGCAG GCCGGCCGGG AGCCGGGTCC CGGGAGCTCC 
ACGAAGGGGC CTGTCCTCCA TGACCAGGAC ACCCGCTGCG CCTTCCTCCC GAGGCCTCCC 
GGGCCTCTCC AGACGCGGCG CTACTGCAGA CACCAGGGCC GCCAAGGGAG CGGACTCGGA 
GCCGGCCCTG GGGCGGGCAC ATGGGCCCCG GCGCCCCCCG GCGTCTCCAA GCCGCGCTGC 
CCGGGTCGGG CCAGGCCAGG GGAGGGACAG CAGCAGGTGA CGACGGCCCG GCCACCGGCT 
ATAAATAGGG GCGCGCGTCA GCCGCGGGCG GGAGCGGCGG CGGCGGGCAG GGGCCCGGGG 
GCCGGGGCCT GGAGGACAGG CGAGGCAGCG GCGAGTGCGG GGCCGGCGGT CGGGGAGGGC 
GGTGCCATGG GGTCGCGGAG GGCCCCCAGC CGGGGCTGGG GCGCGGGTGG GCGGTCGGGG 
GCGGGGGGCG ACGGTGAGGA CGACGGCCCC GTGTGGATCC CCAGCCCAGC CAGCCGGAGC 
TACCTGCTCA GCGTGCGGCC CGAGACCAGC TTATCAAGCA ACCGGTTGTC TCACCCCAGC 
TCTGGAAGGA GCACCTTCTG CTCCATCATT GCTCAGCTCA CAGAGGAGAC CCAGCCGCTA 
TTTGAGACCA CGCTCAAGTC CCGGTCTGTG TCCGAGGACA GCGACGTCAG GTTCACCTGC 
ATCGTCACAG GATACCCAGA GCCAGAGGTG ACCTGGTACA AGGATGATAC GGAGCTGGAC 
CGCTACTGTG GCTTGCCAAA ATATGAGATC ACTCATCAGG GCAACCGCCA CACACTGCAG 
CTGTACAGGT GTCGAGAAGA AGATGCCGCC ATCTACCAGG CCTCTGCCCA GAACAGCAAG 
GGCATTGTGT CCTGCTCAGG GGTCCTGGAG GTGGGCACCA TGACTGAGTA CAAGATCCAC 
CAGCGCTGGT TCGCCAAGTT GAAGCGCAAG GCTGCGGCAA AGCTGCGCGA GATCGAGCAG 
AGCTGGAAGC ACGAGAAGGC GGTGCCTGGG GAGGTCGACA CTCTGCGCAA GCTCAGCCCC 
GACCGCTTCC AGCGAAAGCG GCGATTGAGC GGGGCTCAAG CGCCGGGCCC CTCGGTCCCT 
ACCAGGGAGC CTGAGGGTGG GACCCTGGCG GCTTGGCAGG AGGGAGAGAC TGAGACTGCT 
CAGCACTCAG GTTTGGGCCT GATCAACAGT TTTGCTTCTG GAGAAGTGAC CACCAACGGG 
GAGGCTGCCC CCGAGAATGG AGAGGACGGA GAGCATGGCT TGCTGACATA CATCTGTGAC 
GCCATGGAGC TGGGGCCTCA GAGAGCCCTC AAAGAGGAGA GTGGGGCCAA GAAGAAAAAG 
AAAGATGAGG AATCCAAGCA AGGCCTGCGG AAGCCAGAGT TAGAGAAGGC AGCCCAAAGC 
CGCCGTTCTT CAGAAAACTG CATCCCCAGC TCAGACGAGC CTGACTCCTG TGGGACTCAG 
GGGCCCGTGG GCGTGGAGCA GGTTCAGACC CAGCCCAGAG GCAGGGCTGC ACGGGGGCCT 
GGGTCCTCTG GCACAGATAG TACCAGGAAG CCAGCCTCTG CTGTGGGCAC TCCAGACAAG 
GCCCAGAAGG CCCCTGGCCC AGGCCCAGGC CAGGAAGTGT ATTTCTCCTT GAAGGACATG 
TACCTGGAGA ACACCCAGGC AGTCAGGCCT CTTGGGGAAG AGGGACCCCA GACCCTGAGT 
GTCCGGGCGC CTGGGGAGAG TCCCAAGGGG AAGGCACCCC TCAGGGCTAG AAGCGAGGGG 
GTGCCTGGCG CTCCTGGCCA GCCCACACAC TCCTTGACCC CCCAGCCGAC TAGGCCTTTC 
AACAGAAAGA GATTTGCCCC TCCAAAGCCC AAAGGAGAGG CCACCACTGA CAGCAAGCCC 
ATTTCTTCTC TGAGTCAAGC TCCAGAATGC GGGGCCCAGA GCTTAGGAAA GGCCCCACCT 
CAGGCCTCTG TGCAGGTGCC GACGCCCCCT GCCCGGCGGA GACATGGCAC CCGGGACAGC 
ACGTTGCAGG GGCAAGCAGG CCACAGGACT CCAGGAGAGG TCCTGGAATG CCAGACAACC 
ACGGCTCCTA CCATGTCGGC CAGCAGCAGC TCTGATGTAG CCTCCATTGG GGTTAGCACT 
TCCGGAAGTC AAGGTATCAT TGAACCCATG GATATGGAAA CCCAGGAGGA TGGGAGAACA 
TCTGCTAACC AGAGAACTGG AAGCAAGAAG AATGTGCAGG CAGATGGGAA GATACAAGTG 
GATGGAAGGA CCAGGGGAGA TGGAACACAG ACAGCCCAGA GGACACGTGC AGATAGGAAG 
ACGCAGGTGG ATGCTGGGAC ACAAGAAAGC AAGAGGCCAC AGTCAGACAG GAGTGCACAG 
AAGGGCATGA TGACACAGGG AAGGGCAGAG ACACAGCTAG AAACAACACA GGCAGGTGAG 
AAGATACAGG AAGACAGGAA GGCCCAGGCA GATAAGGGCA CACAGGAAGA CAGAAGGATG 
CAGGGAGAGA AGGGGATGCA GGGAGAGAAG GGGACGCAGT CAGAGGGGAG CGCGCCCACA 
GCCATGGAAG GTCAGTCTGA GCAAGAGGTG GCAACCAGCC TCGGCCCACC ATCCAGAACC 
CCCAAACTCC CACCTACAGC GGGTCCTAGA GCTCCTCTGA ATATTGAATG TTTTGTACAG 
ACCCCAGAAG GGTCTTGTTT CCCAAAAAAA CCTGGTTGCC TGCCCAGATC TGAGGAGGCA 
GTAGTAACAG CCTCCAGGAA CCATGAGCAA ACTGTGCTGG GTCCCCTGTC AGGGAACCTC 
ATGCTCCCAG CACAGCCGCC CCATGAGGGG AGTGTGGAGC AGGTGGGAGG AGAGAGATGC 
CGAGGGCCAC AGTCATCAGG CCCAGTCGAG GCCAAGCAGG AGGACAGCCC GTTCCAGTGC 
CCCAAGGAGG AGCGGCCAGG GGGAGTGCCG TGTATGGATC AGGGTGGCTG TCCTCTAGCT 
GGCCTGAGCC AGGAGGTACC CACGATGCCT TCTCTTCCTG GAACTGGGCT GACAGCTAGC 
CCAAAGGCGG GGCCGTGTAG CACCCCGACT TCTCAGCACG GGAGCACAGC CACCTTCCTG 
CCCTCTGAGG ATCAGGTCCT GATGAGTTCT GCCCCAACAC TGCACCTGGG GCTGGGGACC 
CCCACTCAGA GTCACCCACC AGAAACCATG GCCACCAGCA GTGAGGGGGC CTGCGCCCAG 
GTACCAGATG TGGAGGGGCG GACCCCAGGT CCCCGGAGCT GTGACCCTGG CCTCATAGAT 
TCCCTGAAGA ACTACCTGCT TCTGCTGCTG AAGCTGTCCA GCACAGAGAC AAGTGGAGCA 
GGGGGAGAGT CCCAGGTGGG GGCAGCCACC GGAGGTCTGG TGCCCTCAGC CACTCTGACA 
CCCACTGTGG AAGTGGCTGG GCTTAGTCCC CGGACATCGA GGCGCATCCT GGAGCGTGTG 
GAGAACAACC ACCTGGTGCA GAGTGCACAG ACCCTGCTGC TGAGCCCCTG TACCTCCCGC 
CGCCTCACCG GCCTCCTGGA CCGTGAGGTG CAGGCTGGCC GCCAGGCCCT TGCTGCTGCC 
CGAGGCTCCT GGGGTCCTGG TCCCAGCTCC CTCACTGTCC CTGCCATTGT GGTAGACGAG 
GAGGACCCTG GGCTGGCCTC AGAAGGAGCC AGTGAGGGTG AAGGAGAGGT TTCCCCTGAG 
GGGCCTGGCC TCCTGGGGGC CTCTCAGGAG AGCAGCATGG CTGGTCGACT GGGGGAGGCG 
GGTGGGCAGG CAGCCCCTGG ACAGGGGCCC TCAGCAGAGA GCATAGCCCA GGAGCCCTCC 
CAAGAGGAGA AGTTCCCAGG GGAGGCTCTG ACAGGTCTCC CGGCAGCTAC ACCTGAGGAA 
CTGGCTCTAG GGGCCCGGAG GAAGAGATTT CTCCCTAAGG TCAGAGCAGC AGGAGACGGG 
GAGGCAACCA CACCTGAAGA AAGGGAGAGC CCCACGGTTT CCCCCCGGGG GCCCAGGAAA 
AGCCTGGTGC CTGGGTCCCC AGGGACTCCA GGGCGGGAGA GACGCTCCCC TACGCAGGGC 
AGAAAGGCGA GCATGCTGGA GGTGCCTCGG GCAGAGGAGG AGCTGGCGGC AGGAGACCTG 
GGCCCCAGCC CCAAGGCCGG CGGTCTGGAC ACAGAGGTGG CCCTGGATGA AGGCAAGCAG 
GAGACACTGG CCAAGCCCAG GAAAGCCAAA GACCTGCTGA AAGCCCCACA GGTGATCCGG 
AAGATTCGGG TGGAGCAGTT TCCTGATGCC TCCGGTAGCC TGAAGCTGTG GTGCCAGTTT 
TTCAACATTC TTAGTGACTC AGTCTTGACA TGGGCCAAGG ATCAGCGCCC AGTGGGCGAG 
GTGGGCAGGA GCGCAGGGGA TGAGGGGCCG GCGGCCTTGG CCATCGTGCA GGCCTCCCCC 
GTAGACTGCG GTGTGTATCG GTGCACCATC CACAATGAGC ACGGCTCGGC CTCCACCGAC 
TTCTGCCTCA GCCCTGAGGT GTTGTCAGGA TTCATCTCCA GAGAAGAAGG TGAAGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Potential Oligogenic Etiology of Hypertrophic Cardiomyopathy: A Classic Single-Gene Disorder.
Circulation research120(7)1084-1090(2017 Mar)
Li L,Bainbridge MN,Tan Y,Willerson JT,Marian AJ

Exome-wide association study reveals novel susceptibility genes to sporadic dilated cardiomyopathy.
PloS one12(3)e0172995(2017)
Esslinger U,Garnier S,Korniat A,Proust C,Kararigas G,M?ller-Nurasyid M,Empana JP,Morley MP,Perret C,Stark K,Bick AG,Prasad SK,Kriebel J,Li J,Tiret L,Strauch K,O'Regan DP,Marguiles KB,Seidman JG,Boutouyrie P,Lacolley P,Jouven X,Hengstenberg C,Komajda M,Hakonarson H,Isnard R,Arbustini E,Grallert H,Cook SA,Seidman CE,Regitz-Zagrosek V,Cappola TP,Charron P,Cambien F,Villard E

ALPK3-deficient cardiomyocytes generated from patient-derived induced pluripotent stem cells and mutant human embryonic stem cells display abnormal calcium handling and establish that ALPK3 deficiency underlies familial cardiomyopathy.
European heart journal37(33)2586-90(2016 Sep)
Phelan DG,Anderson DJ,Howden SE,Wong RC,Hickey PF,Pope K,Wilson GR,P?bay A,Davis AM,Petrou S,Elefanty AG,Stanley EG,James PA,Macciocca I,Bahlo M,Cheung MM,Amor DJ,Elliott DA,Lockhart PJ

Biallelic Truncating Mutations in ALPK3 Cause Severe Pediatric Cardiomyopathy.
Journal of the American College of Cardiology67(5)515-25(2016 Feb)
Almomani R,Verhagen JM,Herkert JC,Brosens E,van Spaendonck-Zwarts KY,Asimaki A,van der Zwaag PA,Frohn-Mulder IM,Bertoli-Avella AM,Boven LG,van Slegtenhorst MA,van der Smagt JJ,van IJcken WF,Timmer B,van Stuijvenberg M,Verdijk RM,Saffitz JE,du Plessis FA,Michels M,Hofstra RM,Sinke RJ,van Tintelen JP,Wessels MW,Jongbloed JD,van de Laar IM

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ALPK3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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