TRMT61A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRMT61A
Entrez Gene ID	510283
Full Name	tRNA methyltransferase 61A
Synonyms	GCD14,TRM61
General protein information	Preferred Names tRNA methyltransferase 61A Names tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A mRNA methyladenosine-N(1)-methyltransferase catalytic subunit TRMT61A tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRM61 tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRMT61A tRNA methyltransferase 61 homolog A tRNA(m1A58)-methyltransferase subunit TRM61 tRNA(m1A58)-methyltransferase subunit TRMT61A tRNA(m1A58)MTase subunit TRM61 tRNA(m1A58)MTase subunit TRMT61A
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005222179.4	XP_005222236.1	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X1
NM_001098927.2	NP_001092397.1	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A
XM_005222179.3	XP_005222236.1	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRMT61A	NP_689520.2
	TRMT61A	XP_001083535.1
Mus musculus (house mouse)	Trmt61a	NP_001093262.1
Gallus gallus (chicken)	TRMT61A	XP_421386.4
Kluyveromyces lactis	KLLA0E15225g	XP_454636.1
Rattus norvegicus (Norway rat)	Trmt61a	NP_001007707.1
Danio rerio (zebrafish)	trmt61a	NP_001002321.1
Saccharomyces cerevisiae (baker's yeast)	GCD14	NP_012410.1
Arabidopsis thaliana (thale cress)	AT5G14600	NP_568302.1
Pan troglodytes (chimpanzee)	TRMT61A	XP_510186.3
Bos taurus (cattle)	TRMT61A	NP_001092397.1
Drosophila melanogaster (fruit fly)	CG14544	NP_651461.1
Caenorhabditis elegans (roundworm)	CELE_W02A11.1	NP_493229.1
Oryza sativa (rice)	Os04g0326300	NP_001052474.2
Canis lupus familiaris (dog)	TRMT61A	XP_547993.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003966	XP_318418.4
Eremothecium gossypii	AGOS_AFL214C	NP_985336.1
Schizosaccharomyces pombe (fission yeast)	cpd1	NP_593567.1
Xenopus tropicalis (tropical clawed frog)	trmt61a	NP_001005009.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Discovery and characterization of 91 novel transcripts expressed in cattle placenta.
Kumar CG, Larson JH, Band MR, Lewin HA
BMC genomics8113(2007 May)

Discovery of eight novel divergent homologs expressed in cattle placenta.
Larson JH, Kumar CG, Everts RE, Green CA, Everts-van der Wind A, Band MR, Lewin HA
Physiological genomics25(3)405-13(2006 May)

The bipartite structure of the tRNA m1A58 methyltransferase from S. cerevisiae is conserved in humans.
Ozanick S, Krecic A, Andersland J, Anderson JT
RNA (New York, N.Y.)11(8)1281-90(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Shows conservation of yeast TRM61 (GCD14) in mouse, human and Drosophila, and demonstrates functional activity of the human ortholog.
Title: The bipartite structure of the tRNA m1A58 methyltransferase from S. cerevisiae is conserved in humans.

The following TRMT61A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRMT61A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa00492	NM_001098927.2 Latest version!	Bos taurus tRNA methyltransferase 61A (TRMT61A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00
OBa31097	XM_005222179.4 Latest version!	Bos taurus tRNA methyltransferase 61A (TRMT61A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00
View Old Accession Versions >>
OBa31097	XM_005222179.3	Bos taurus tRNA methyltransferase 61A (TRMT61A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRMT61A ( NM_001098927.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001092397.1 Bos taurus tRNA methyltransferase 61A (TRMT61A), mRNA.

Price & Availability↑

CloneID	OBa00492
Clone ID Related Accession (Same CDS sequence)	NM_001098927.2
Accession Version	NM_001098927.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Bos taurus(cattle)
Product	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC146157.1. On Aug 31, 2012 this sequence version replaced NM_001098927.1. ##Evidence-Data-START## Transcript exon combination :: BC146157.1, EE248963.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTTCG TGGCCTACGA GGAGCTGATC AAAGAGGGTG ACACAGCCAT CCTGTCCCTG 
GGCCATGGCG CGATGGTGGC CGTGCGCGTG CAGCGGGGGG CCCAGACCCA AACCCGGCAC 
GGCGTGCTGC GGCACTCAGT AGACCTCATC GGCCGCCCCT TCGGCTCCAA GGTGACCTGC 
GGACGAGGCG GCTGGGTGTA TGTGCTGCAC CCCACGCCGG AGCTCTGGAC ACTGAACCTG 
CCGCACCGCA CCCAGATCCT CTACTCCACC GACATCGCCC TGCTCACCAT GATGCTGGAG 
CTTCGGCCTG GCTCCGTGGT CTGTGAATCC GGCACTGGCA GCGGCTCCGT GTCCCATGCC 
ATCATCCGCA CCATCGCGCC CACGGGCCAC CTGCACACGG TGGAGTTCCA CCAGCAGCGG 
GCGGAGAGGG CGCGGGAGGA GTTCCAGGAG CACCGTGTGG GCCGCTGGGT GACCGTGCTG 
AACCAGGACG TGTGCCGCAG CGGCTTTGGC GTGAGCCACG TGGCGGACGC CGTCTTCCTG 
GACATCCCCT CGCCTTGGGA GGCTGTGGGG CATGCCTGGG ATGCCCTCAA GGTCGAAGGT 
GGGCGCTTCT GCTCCTTCTC ACCCTGCATT GAGCAGGTGC AGCGCACATG CCAGGCGCTG 
GCAGCCTGCG GCTTCTCGGA GCTGAGCACC CTGGAGGTGC TGCCGCAGGT CTACAATGTG 
CGCACCGTCA GCCTGCCCGT GCCCGACCTG GGGGCCCGCC CCGGCCCCGA CGCCGCCCCC 
TTCCGCAGCG GCACGCCCAT GAAGGAGACC GTGGGTCACA CCGGCTACCT GACCTTCGCC 
ACCAAGACTC CGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092397.1
CDS	88..945
Translation	MSFVAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRGGWVYVLHPTPELWTLNLPHRTQILYSTDIALLTMMLELRPGSVVCESGTGSGSVSHAIIRTIAPTGHLHTVEFHQQRAERAREEFQEHRVGRWVTVLNQDVCRSGFGVSHVADAVFLDIPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAACGFSELSTLEVLPQVYNVRTVSLPVPDLGARPGPDAAPFRSGTPMKETVGHTGYLTFATKTPG

Target ORF information:

RefSeq Version	NM_001098927.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 61A (TRMT61A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098927.2

ORF Insert Sequence:

ATGAGCTTCG TGGCCTACGA GGAGCTGATC AAAGAGGGTG ACACAGCCAT CCTGTCCCTG 
GGCCATGGCG CGATGGTGGC CGTGCGCGTG CAGCGGGGGG CCCAGACCCA AACCCGGCAC 
GGCGTGCTGC GGCACTCAGT AGACCTCATC GGCCGCCCCT TCGGCTCCAA GGTGACCTGC 
GGACGAGGCG GCTGGGTGTA TGTGCTGCAC CCCACGCCGG AGCTCTGGAC ACTGAACCTG 
CCGCACCGCA CCCAGATCCT CTACTCCACC GACATCGCCC TGCTCACCAT GATGCTGGAG 
CTTCGGCCTG GCTCCGTGGT CTGTGAATCC GGCACTGGCA GCGGCTCCGT GTCCCATGCC 
ATCATCCGCA CCATCGCGCC CACGGGCCAC CTGCACACGG TGGAGTTCCA CCAGCAGCGG 
GCGGAGAGGG CGCGGGAGGA GTTCCAGGAG CACCGTGTGG GCCGCTGGGT GACCGTGCTG 
AACCAGGACG TGTGCCGCAG CGGCTTTGGC GTGAGCCACG TGGCGGACGC CGTCTTCCTG 
GACATCCCCT CGCCTTGGGA GGCTGTGGGG CATGCCTGGG ATGCCCTCAA GGTCGAAGGT 
GGGCGCTTCT GCTCCTTCTC ACCCTGCATT GAGCAGGTGC AGCGCACATG CCAGGCGCTG 
GCAGCCTGCG GCTTCTCGGA GCTGAGCACC CTGGAGGTGC TGCCGCAGGT CTACAATGTG 
CGCACCGTCA GCCTGCCCGT GCCCGACCTG GGGGCCCGCC CCGGCCCCGA CGCCGCCCCC 
TTCCGCAGCG GCACGCCCAT GAAGGAGACC GTGGGTCACA CCGGCTACCT GACCTTCGCC 
ACCAAGACTC CGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT61A ( XM_005222179.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222236.1 Bos taurus tRNA methyltransferase 61A (TRMT61A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa31097
Clone ID Related Accession (Same CDS sequence)	XM_005222179.4 , XM_005222179.3
Accession Version	XM_005222179.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222179.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTGT TTATTGCCTG CTGCACGGCC CCAGCCTCCG CTTGGGGACC CGTGCATTTG 
CATCGCCCCG TCACCCGTCT GCCCAGGAGC CCGCCACGGG GGAAGGTCCC CGGCACCTGT 
GGGACCAGCA GCACCATGAG CTTCGTGGCC TACGAGGAGC TGATCAAAGA GGGTGACACA 
GCCATCCTGT CCCTGGGCCA TGGCGCGATG GTGGCCGTGC GCGTGCAGCC GGGGGCCCAG 
ACCCAAACCC GGCACGGCGT GCTGCGGCAC TCAGTAGACC TCATCGGCCG CCCCTTCGGC 
TCCAAGGTGA CCTGCGGACG AGGCGGCTGG GTGTATGTGC TGCACCCCAC GCCGGAGCTC 
TGGACACTGA ACCTGCCGCA CCGCACCCAG ATCCTCTACT CCACCGACAT CGCCCTGCTC 
ACCATGATGC TGGAGCTTCG GCCTGGCTCC GTGGTCTGTG AATCCGGCAC TGGCAGCGGC 
TCCGTGTCCC ATGCCATCAT CCGCACCATC GCGCCCACGG GCCACCTGCA CACGGTGGAG 
TTCCACCAGC AGCGGGCGGA GAGGGCGCGG GAGGAGTTCC AGGAGCACCG TGTGGGCCGC 
TGGGTGACCG TGCTGAACCA GGACGTGTGC CGCAGCGGCT TTGGCGTGAG CCACGTGGCG 
GACGCCGTCT TCCTGGACAT CCCCTCGCCT TGGGAGGCTG TGGGGCATGC CTGGGATGCC 
CTCAAGGTCG AAGGTGGGCG CTTCTGCTCC TTCTCACCCT GCATTGAGCA GGTGCAGCGC 
ACATGCCAGG CGCTGGCAGC CTGCGGCTTC TCGGAGCTGA GCACCCTGGA GGTGCTGCCG 
CAGGTCTACA ATGTGCGCAC CGTCAGCCTG CCCGTGCCCG ACCTGGGGGC CCGCCCCGGC 
CCCGACGCCG CCCCCTTCCG CAGCGGCACG CCCATGAAGG AGACCGTGGG TCACACCGGC 
TACCTGACCT TCGCCACCAA GACTCCGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222236.1
CDS	143..1135
Translation	MLLFIACCTAPASAWGPVHLHRPVTRLPRSPPRGKVPGTCGTSSTMSFVAYEELIKEGDTAILSLGHGAMVAVRVQPGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRGGWVYVLHPTPELWTLNLPHRTQILYSTDIALLTMMLELRPGSVVCESGTGSGSVSHAIIRTIAPTGHLHTVEFHQQRAERAREEFQEHRVGRWVTVLNQDVCRSGFGVSHVADAVFLDIPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAACGFSELSTLEVLPQVYNVRTVSLPVPDLGARPGPDAAPFRSGTPMKETVGHTGYLTFATKTPG

Target ORF information:

RefSeq Version	XM_005222179.4
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 61A (TRMT61A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222179.4

ORF Insert Sequence:

ATGTTGCTGT TTATTGCCTG CTGCACGGCC CCAGCCTCCG CTTGGGGACC CGTGCATTTG 
CATCGCCCCG TCACCCGTCT GCCCAGGAGC CCGCCACGGG GGAAGGTCCC CGGCACCTGT 
GGGACCAGCA GCACCATGAG CTTCGTGGCC TACGAGGAGC TGATCAAAGA GGGTGACACA 
GCCATCCTGT CCCTGGGCCA TGGCGCGATG GTGGCCGTGC GCGTGCAGCC GGGGGCCCAG 
ACCCAAACCC GGCACGGCGT GCTGCGGCAC TCAGTAGACC TCATCGGCCG CCCCTTCGGC 
TCCAAGGTGA CCTGCGGACG AGGCGGCTGG GTGTATGTGC TGCACCCCAC GCCGGAGCTC 
TGGACACTGA ACCTGCCGCA CCGCACCCAG ATCCTCTACT CCACCGACAT CGCCCTGCTC 
ACCATGATGC TGGAGCTTCG GCCTGGCTCC GTGGTCTGTG AATCCGGCAC TGGCAGCGGC 
TCCGTGTCCC ATGCCATCAT CCGCACCATC GCGCCCACGG GCCACCTGCA CACGGTGGAG 
TTCCACCAGC AGCGGGCGGA GAGGGCGCGG GAGGAGTTCC AGGAGCACCG TGTGGGCCGC 
TGGGTGACCG TGCTGAACCA GGACGTGTGC CGCAGCGGCT TTGGCGTGAG CCACGTGGCG 
GACGCCGTCT TCCTGGACAT CCCCTCGCCT TGGGAGGCTG TGGGGCATGC CTGGGATGCC 
CTCAAGGTCG AAGGTGGGCG CTTCTGCTCC TTCTCACCCT GCATTGAGCA GGTGCAGCGC 
ACATGCCAGG CGCTGGCAGC CTGCGGCTTC TCGGAGCTGA GCACCCTGGA GGTGCTGCCG 
CAGGTCTACA ATGTGCGCAC CGTCAGCCTG CCCGTGCCCG ACCTGGGGGC CCGCCCCGGC 
CCCGACGCCG CCCCCTTCCG CAGCGGCACG CCCATGAAGG AGACCGTGGG TCACACCGGC 
TACCTGACCT TCGCCACCAA GACTCCGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT61A ( XM_005222179.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222236.1 Bos taurus tRNA methyltransferase 61A (TRMT61A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa31097
Clone ID Related Accession (Same CDS sequence)	XM_005222179.4 , XM_005222179.3
Accession Version	XM_005222179.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104538.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222179.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTGT TTATTGCCTG CTGCACGGCC CCAGCCTCCG CTTGGGGACC CGTGCATTTG 
CATCGCCCCG TCACCCGTCT GCCCAGGAGC CCGCCACGGG GGAAGGTCCC CGGCACCTGT 
GGGACCAGCA GCACCATGAG CTTCGTGGCC TACGAGGAGC TGATCAAAGA GGGTGACACA 
GCCATCCTGT CCCTGGGCCA TGGCGCGATG GTGGCCGTGC GCGTGCAGCC GGGGGCCCAG 
ACCCAAACCC GGCACGGCGT GCTGCGGCAC TCAGTAGACC TCATCGGCCG CCCCTTCGGC 
TCCAAGGTGA CCTGCGGACG AGGCGGCTGG GTGTATGTGC TGCACCCCAC GCCGGAGCTC 
TGGACACTGA ACCTGCCGCA CCGCACCCAG ATCCTCTACT CCACCGACAT CGCCCTGCTC 
ACCATGATGC TGGAGCTTCG GCCTGGCTCC GTGGTCTGTG AATCCGGCAC TGGCAGCGGC 
TCCGTGTCCC ATGCCATCAT CCGCACCATC GCGCCCACGG GCCACCTGCA CACGGTGGAG 
TTCCACCAGC AGCGGGCGGA GAGGGCGCGG GAGGAGTTCC AGGAGCACCG TGTGGGCCGC 
TGGGTGACCG TGCTGAACCA GGACGTGTGC CGCAGCGGCT TTGGCGTGAG CCACGTGGCG 
GACGCCGTCT TCCTGGACAT CCCCTCGCCT TGGGAGGCTG TGGGGCATGC CTGGGATGCC 
CTCAAGGTCG AAGGTGGGCG CTTCTGCTCC TTCTCACCCT GCATTGAGCA GGTGCAGCGC 
ACATGCCAGG CGCTGGCAGC CTGCGGCTTC TCGGAGCTGA GCACCCTGGA GGTGCTGCCG 
CAGGTCTACA ATGTGCGCAC CGTCAGCCTG CCCGTGCCCG ACCTGGGGGC CCGCCCCGGC 
CCCGACGCCG CCCCCTTCCG CAGCGGCACG CCCATGAAGG AGACCGTGGG TCACACCGGC 
TACCTGACCT TCGCCACCAA GACTCCGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222236.1
CDS	129..1121
Translation	MLLFIACCTAPASAWGPVHLHRPVTRLPRSPPRGKVPGTCGTSSTMSFVAYEELIKEGDTAILSLGHGAMVAVRVQPGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRGGWVYVLHPTPELWTLNLPHRTQILYSTDIALLTMMLELRPGSVVCESGTGSGSVSHAIIRTIAPTGHLHTVEFHQQRAERAREEFQEHRVGRWVTVLNQDVCRSGFGVSHVADAVFLDIPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAACGFSELSTLEVLPQVYNVRTVSLPVPDLGARPGPDAAPFRSGTPMKETVGHTGYLTFATKTPG

Target ORF information:

RefSeq Version	XM_005222179.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 61A (TRMT61A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222179.3

ORF Insert Sequence:

ATGTTGCTGT TTATTGCCTG CTGCACGGCC CCAGCCTCCG CTTGGGGACC CGTGCATTTG 
CATCGCCCCG TCACCCGTCT GCCCAGGAGC CCGCCACGGG GGAAGGTCCC CGGCACCTGT 
GGGACCAGCA GCACCATGAG CTTCGTGGCC TACGAGGAGC TGATCAAAGA GGGTGACACA 
GCCATCCTGT CCCTGGGCCA TGGCGCGATG GTGGCCGTGC GCGTGCAGCC GGGGGCCCAG 
ACCCAAACCC GGCACGGCGT GCTGCGGCAC TCAGTAGACC TCATCGGCCG CCCCTTCGGC 
TCCAAGGTGA CCTGCGGACG AGGCGGCTGG GTGTATGTGC TGCACCCCAC GCCGGAGCTC 
TGGACACTGA ACCTGCCGCA CCGCACCCAG ATCCTCTACT CCACCGACAT CGCCCTGCTC 
ACCATGATGC TGGAGCTTCG GCCTGGCTCC GTGGTCTGTG AATCCGGCAC TGGCAGCGGC 
TCCGTGTCCC ATGCCATCAT CCGCACCATC GCGCCCACGG GCCACCTGCA CACGGTGGAG 
TTCCACCAGC AGCGGGCGGA GAGGGCGCGG GAGGAGTTCC AGGAGCACCG TGTGGGCCGC 
TGGGTGACCG TGCTGAACCA GGACGTGTGC CGCAGCGGCT TTGGCGTGAG CCACGTGGCG 
GACGCCGTCT TCCTGGACAT CCCCTCGCCT TGGGAGGCTG TGGGGCATGC CTGGGATGCC 
CTCAAGGTCG AAGGTGGGCG CTTCTGCTCC TTCTCACCCT GCATTGAGCA GGTGCAGCGC 
ACATGCCAGG CGCTGGCAGC CTGCGGCTTC TCGGAGCTGA GCACCCTGGA GGTGCTGCCG 
CAGGTCTACA ATGTGCGCAC CGTCAGCCTG CCCGTGCCCG ACCTGGGGGC CCGCCCCGGC 
CCCGACGCCG CCCCCTTCCG CAGCGGCACG CCCATGAAGG AGACCGTGGG TCACACCGGC 
TACCTGACCT TCGCCACCAA GACTCCGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Discovery and characterization of 91 novel transcripts expressed in cattle placenta.
BMC genomics8113(2007 May)
Kumar CG,Larson JH,Band MR,Lewin HA

Discovery of eight novel divergent homologs expressed in cattle placenta.
Physiological genomics25(3)405-13(2006 May)
Larson JH,Kumar CG,Everts RE,Green CA,Everts-van der Wind A,Band MR,Lewin HA

The bipartite structure of the tRNA m1A58 methyltransferase from S. cerevisiae is conserved in humans.
RNA (New York, N.Y.)11(8)1281-90(2005 Aug)
Ozanick S,Krecic A,Andersland J,Anderson JT

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TRMT61A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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