TRMT61A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRMT61A
Entrez Gene ID	115708
Full Name	tRNA methyltransferase 61A
Synonyms	C14orf172,GCD14,Gcd14p,TRM61,hTRM61
General protein information	Preferred Names tRNA methyltransferase 61A Names tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A mRNA methyladenosine-N(1)-methyltransferase catalytic subunit TRMT61A tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRM61 tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRMT61A tRNA methyltransferase 61 homolog A tRNA(m1A58)-methyltransferase subunit TRM61 tRNA(m1A58)-methyltransferase subunit TRMT61A tRNA(m1A58)MTase subunit TRM61 tRNA(m1A58)MTase subunit TRMT61A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q32.33

mRNA and Protein(s)

mRNA	Protein	Name
NM_152307.2	NP_689520.2	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A
NM_152307.3	NP_689520.2	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRMT61A	NP_689520.2
	TRMT61A	XP_001083535.1
Mus musculus (house mouse)	Trmt61a	NP_001093262.1
Gallus gallus (chicken)	TRMT61A	XP_421386.4
Kluyveromyces lactis	KLLA0E15225g	XP_454636.1
Rattus norvegicus (Norway rat)	Trmt61a	NP_001007707.1
Danio rerio (zebrafish)	trmt61a	NP_001002321.1
Saccharomyces cerevisiae (baker's yeast)	GCD14	NP_012410.1
Arabidopsis thaliana (thale cress)	AT5G14600	NP_568302.1
Pan troglodytes (chimpanzee)	TRMT61A	XP_510186.3
Bos taurus (cattle)	TRMT61A	NP_001092397.1
Drosophila melanogaster (fruit fly)	CG14544	NP_651461.1
Caenorhabditis elegans (roundworm)	CELE_W02A11.1	NP_493229.1
Oryza sativa (rice)	Os04g0326300	NP_001052474.2
Canis lupus familiaris (dog)	TRMT61A	XP_547993.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003966	XP_318418.4
Eremothecium gossypii	AGOS_AFL214C	NP_985336.1
Schizosaccharomyces pombe (fission yeast)	cpd1	NP_593567.1
Xenopus tropicalis (tropical clawed frog)	trmt61a	NP_001005009.1

Gene Ontology
Bibliography

Related articles in PubMed

Base-Resolution Mapping Reveals Distinct m
Li X, Xiong X, Zhang M, Wang K, Chen Y, Zhou J, Mao Y, Lv J, Yi D, Chen XW, Wang C, Qian SB, Yi C
Molecular cell68(5)993-1005.e9(2017 Dec)

The m1A landscape on cytosolic and mitochondrial mRNA at single-base resolution.
Safra M, Sas-Chen A, Nir R, Winkler R, Nachshon A, Bar-Yaacov D, Erlacher M, Rossmanith W, Stern-Ginossar N, Schwartz S
Nature551(7679)251-255(2017 Nov)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

TRM6/61 connects PKC? with translational control through tRNAi(Met) stabilization: impact on tumorigenesis.
Macari F, El-Houfi Y, Boldina G, Xu H, Khoury-Hanna S, Ollier J, Yazdani L, Zheng G, Bi?che I, Legrand N, Paulet D, Durrieu S, Bystr?m A, Delbecq S, Lapeyre B, Bauchet L, Pannequin J, Hollande F, Pan T, Teichmann M, Vagner S, David A, Choquet A, Joubert D
Oncogene35(14)1785-96(2016 Apr)

Crystal Structure of the Human tRNA m(1)A58 Methyltransferase-tRNA(3)(Lys) Complex: Refolding of Substrate tRNA Allows Access to the Methylation Target.
Finer-Moore J, Czudnochowski N, O'Connell JD 3rd, Wang AL, Stroud RM
Journal of molecular biology427(24)3862-76(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we suggest that PKCA tightly controls TRM6/61 activity to prevent translation deregulation that would favor neoplastic development.
Title: TRM6/61 connects PKC? with translational control through tRNAi(Met) stabilization: impact on tumorigenesis.

The TRM61 heterotetramer is the only active species, both subunits are required for orientating tRNA for catalysis at Trm61.
Title: Crystal Structure of the Human tRNA m(1)A58 Methyltransferase-tRNA(3)(Lys) Complex: Refolding of Substrate tRNA Allows Access to the Methylation Target.

The following TRMT61A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRMT61A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu13512	NM_152307.2 Latest version!	Homo sapiens tRNA methyltransferase 61A (TRMT61A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu107741	NM_152307.3 Latest version!	Homo sapiens tRNA methyltransferase 61A (TRMT61A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRMT61A ( NM_152307.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_689520.2 Homo sapiens tRNA methyltransferase 61A (TRMT61A), mRNA.

Price & Availability↑

CloneID	OHu13512
Clone ID Related Accession (Same CDS sequence)	NM_152307.2
Accession Version	NM_152307.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB496873.1, BC010167.2, AL133367.4, AL139300.6 and BQ015210.1. On Jan 19, 2007 this sequence version replaced NM_152307.1. ##Evidence-Data-START## Transcript exon combination :: AK097771.1, SRR1660807.51968.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTTCG TGGCATACGA GGAGCTGATC AAGGAGGGTG ACACGGCCAT CCTGTCACTG 
GGCCATGGTG CAATGGTGGC AGTGCGTGTG CAGCGTGGGG CACAGACCCA GACCCGGCAT 
GGTGTCCTGC GGCACTCAGT TGACCTTATC GGCCGCCCCT TCGGCTCCAA GGTGACGTGC 
GGCCGAGGTG GCTGGGTGTA TGTGCTGCAC CCCACGCCCG AGCTCTGGAC GCTGAACCTG 
CCGCACCGCA CGCAGATCCT CTACTCCACA GACATCGCCC TCATCACCAT GATGTTGGAG 
CTTCGGCCCG GCTCTGTGGT CTGTGAGTCT GGCACCGGCA GTGGCTCTGT GTCCCACGCC 
ATCATCCGCA CCATTGCACC CACGGGTCAC CTGCACACGG TGGAGTTCCA CCAGCAGCGG 
GCAGAGAAGG CCCGGGAGGA GTTCCAGGAG CACCGTGTGG GCCGCTGGGT GACTGTGCGC 
ACCCAGGACG TGTGCCGCAG TGGCTTTGGC GTGAGCCACG TGGCCGACGC CGTCTTCCTG 
GACATCCCAT CACCCTGGGA GGCCGTGGGC CACGCCTGGG ACGCCCTCAA GGTCGAAGGC 
GGGCGCTTCT GCTCCTTCTC ACCGTGCATC GAGCAGGTGC AACGCACATG CCAGGCGCTG 
GCAGCGCGCG GCTTCTCAGA GCTGAGCACC CTGGAGGTGC TGCCACAGGT CTACAACGTG 
CGCACTGTCA GCCTGCCACC GCCCGACCTG GGCACAGGCA CAGATGGCCC TGCCGGCTCC 
GACACCAGCC CCTTCCGCAG CGGCACGCCC ATGAAGGAGG CCGTGGGCCA CACCGGCTAC 
CTGACCTTCG CCACCAAGAC CCCAGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689520.2
CDS	120..989
Translation	MSFVAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRGGWVYVLHPTPELWTLNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIRTIAPTGHLHTVEFHQQRAEKAREEFQEHRVGRWVTVRTQDVCRSGFGVSHVADAVFLDIPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAARGFSELSTLEVLPQVYNVRTVSLPPPDLGTGTDGPAGSDTSPFRSGTPMKEAVGHTGYLTFATKTPG

Target ORF information:

RefSeq Version	NM_152307.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 61A (TRMT61A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152307.2

ORF Insert Sequence:

ATGAGCTTCG TGGCATACGA GGAGCTGATC AAGGAGGGTG ACACGGCCAT CCTGTCACTG 
GGCCATGGTG CAATGGTGGC AGTGCGTGTG CAGCGTGGGG CACAGACCCA GACCCGGCAT 
GGTGTCCTGC GGCACTCAGT TGACCTTATC GGCCGCCCCT TCGGCTCCAA GGTGACGTGC 
GGCCGAGGTG GCTGGGTGTA TGTGCTGCAC CCCACGCCCG AGCTCTGGAC GCTGAACCTG 
CCGCACCGCA CGCAGATCCT CTACTCCACA GACATCGCCC TCATCACCAT GATGTTGGAG 
CTTCGGCCCG GCTCTGTGGT CTGTGAGTCT GGCACCGGCA GTGGCTCTGT GTCCCACGCC 
ATCATCCGCA CCATTGCACC CACGGGTCAC CTGCACACGG TGGAGTTCCA CCAGCAGCGG 
GCAGAGAAGG CCCGGGAGGA GTTCCAGGAG CACCGTGTGG GCCGCTGGGT GACTGTGCGC 
ACCCAGGACG TGTGCCGCAG TGGCTTTGGC GTGAGCCACG TGGCCGACGC CGTCTTCCTG 
GACATCCCAT CACCCTGGGA GGCCGTGGGC CACGCCTGGG ACGCCCTCAA GGTCGAAGGC 
GGGCGCTTCT GCTCCTTCTC ACCGTGCATC GAGCAGGTGC AACGCACATG CCAGGCGCTG 
GCAGCGCGCG GCTTCTCAGA GCTGAGCACC CTGGAGGTGC TGCCACAGGT CTACAACGTG 
CGCACTGTCA GCCTGCCACC GCCCGACCTG GGCACAGGCA CAGATGGCCC TGCCGGCTCC 
GACACCAGCC CCTTCCGCAG CGGCACGCCC ATGAAGGAGG CCGTGGGCCA CACCGGCTAC 
CTGACCTTCG CCACCAAGAC CCCAGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT61A ( NM_152307.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_689520.2 Homo sapiens tRNA methyltransferase 61A (TRMT61A), mRNA.

Price & Availability↑

CloneID	OHu107741
Clone ID Related Accession (Same CDS sequence)	NM_152307.3
Accession Version	NM_152307.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL133367.4 and AL139300.6. On Nov 23, 2018 this sequence version replaced NM_152307.2. ##Evidence-Data-START## Transcript exon combination :: ERR279830.519.1, SRR1660807.51968.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000389749.9/ ENSP00000374399.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCTTCG TGGCATACGA GGAGCTGATC AAGGAGGGTG ACACGGCCAT CCTGTCACTG 
GGCCATGGTG CAATGGTGGC GGTGCGTGTG CAGCGTGGGG CACAGACCCA GACCCGGCAT 
GGTGTCCTGC GGCACTCAGT TGACCTTATC GGCCGCCCCT TCGGCTCCAA GGTGACGTGC 
GGCCGAGGTG GCTGGGTGTA TGTGCTGCAC CCCACGCCCG AGCTCTGGAC GCTGAACCTG 
CCGCACCGCA CGCAGATCCT CTACTCCACA GACATCGCCC TCATCACCAT GATGTTGGAG 
CTTCGGCCCG GCTCTGTGGT CTGTGAGTCT GGCACCGGCA GTGGCTCTGT GTCCCACGCC 
ATCATCCGCA CCATTGCACC CACGGGTCAC CTGCACACGG TGGAGTTCCA CCAGCAGCGG 
GCAGAGAAGG CCCGGGAGGA GTTCCAGGAG CACCGTGTGG GCCGCTGGGT GACTGTGCGC 
ACCCAGGACG TGTGCCGCAG TGGCTTTGGC GTGAGCCACG TGGCCGACGC CGTCTTCCTG 
GACATCCCAT CACCCTGGGA GGCCGTGGGC CACGCCTGGG ACGCCCTCAA GGTCGAAGGC 
GGGCGCTTCT GCTCCTTCTC ACCGTGCATC GAGCAGGTGC AACGCACATG CCAGGCGCTG 
GCAGCGCGCG GCTTCTCAGA GCTGAGCACC CTGGAGGTGC TGCCACAGGT CTACAACGTG 
CGCACTGTCA GCCTGCCACC GCCCGACCTG GGCACAGGCA CAGATGGCCC TGCCGGCTCC 
GACACCAGCC CCTTCCGCAG CGGCACGCCC ATGAAGGAGG CCGTGGGCCA CACCGGCTAC 
CTGACCTTCG CCACCAAGAC CCCAGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689520.2
CDS	96..965
Translation	MSFVAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRGGWVYVLHPTPELWTLNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIRTIAPTGHLHTVEFHQQRAEKAREEFQEHRVGRWVTVRTQDVCRSGFGVSHVADAVFLDIPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAARGFSELSTLEVLPQVYNVRTVSLPPPDLGTGTDGPAGSDTSPFRSGTPMKEAVGHTGYLTFATKTPG

Target ORF information:

RefSeq Version	NM_152307.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 61A (TRMT61A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152307.3

ORF Insert Sequence:

ATGAGCTTCG TGGCATACGA GGAGCTGATC AAGGAGGGTG ACACGGCCAT CCTGTCACTG 
GGCCATGGTG CAATGGTGGC GGTGCGTGTG CAGCGTGGGG CACAGACCCA GACCCGGCAT 
GGTGTCCTGC GGCACTCAGT TGACCTTATC GGCCGCCCCT TCGGCTCCAA GGTGACGTGC 
GGCCGAGGTG GCTGGGTGTA TGTGCTGCAC CCCACGCCCG AGCTCTGGAC GCTGAACCTG 
CCGCACCGCA CGCAGATCCT CTACTCCACA GACATCGCCC TCATCACCAT GATGTTGGAG 
CTTCGGCCCG GCTCTGTGGT CTGTGAGTCT GGCACCGGCA GTGGCTCTGT GTCCCACGCC 
ATCATCCGCA CCATTGCACC CACGGGTCAC CTGCACACGG TGGAGTTCCA CCAGCAGCGG 
GCAGAGAAGG CCCGGGAGGA GTTCCAGGAG CACCGTGTGG GCCGCTGGGT GACTGTGCGC 
ACCCAGGACG TGTGCCGCAG TGGCTTTGGC GTGAGCCACG TGGCCGACGC CGTCTTCCTG 
GACATCCCAT CACCCTGGGA GGCCGTGGGC CACGCCTGGG ACGCCCTCAA GGTCGAAGGC 
GGGCGCTTCT GCTCCTTCTC ACCGTGCATC GAGCAGGTGC AACGCACATG CCAGGCGCTG 
GCAGCGCGCG GCTTCTCAGA GCTGAGCACC CTGGAGGTGC TGCCACAGGT CTACAACGTG 
CGCACTGTCA GCCTGCCACC GCCCGACCTG GGCACAGGCA CAGATGGCCC TGCCGGCTCC 
GACACCAGCC CCTTCCGCAG CGGCACGCCC ATGAAGGAGG CCGTGGGCCA CACCGGCTAC 
CTGACCTTCG CCACCAAGAC CCCAGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Base-Resolution Mapping Reveals Distinct m
Molecular cell68(5)993-1005.e9(2017 Dec)
Li X,Xiong X,Zhang M,Wang K,Chen Y,Zhou J,Mao Y,Lv J,Yi D,Chen XW,Wang C,Qian SB,Yi C

The m1A landscape on cytosolic and mitochondrial mRNA at single-base resolution.
Nature551(7679)251-255(2017 Nov)
Safra M,Sas-Chen A,Nir R,Winkler R,Nachshon A,Bar-Yaacov D,Erlacher M,Rossmanith W,Stern-Ginossar N,Schwartz S

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

TRM6/61 connects PKC? with translational control through tRNAi(Met) stabilization: impact on tumorigenesis.
Oncogene35(14)1785-96(2016 Apr)
Macari F,El-Houfi Y,Boldina G,Xu H,Khoury-Hanna S,Ollier J,Yazdani L,Zheng G,Bi?che I,Legrand N,Paulet D,Durrieu S,Bystr?m A,Delbecq S,Lapeyre B,Bauchet L,Pannequin J,Hollande F,Pan T,Teichmann M,Vagner S,David A,Choquet A,Joubert D

Crystal Structure of the Human tRNA m(1)A58 Methyltransferase-tRNA(3)(Lys) Complex: Refolding of Substrate tRNA Allows Access to the Methylation Target.
Journal of molecular biology427(24)3862-76(2015 Dec)
Finer-Moore J,Czudnochowski N,O'Connell JD 3rd,Wang AL,Stroud RM

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TRMT61A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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