Trmt61a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trmt61a
Entrez Gene ID	314462
Full Name	tRNA methyltransferase 61A
Synonyms	Gcd14,RGD1359191,Trm61
General protein information	Preferred Names tRNA methyltransferase 61A Names tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A GCD14 and PCMT domain containing protein RGD1359191 GCD14/PCMT domain containing protein RGD1359191 mRNA methyladenosine-N(1)-methyltransferase catalytic subunit TRMT61A tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRM61 tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRMT61A tRNA methyltransferase 61 homolog A tRNA(m1A58)-methyltransferase subunit TRM61 tRNA(m1A58)-methyltransferase subunit TRMT61A tRNA(m1A58)MTase subunit TRM61 tRNA(m1A58)MTase subunit TRMT61A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q32

mRNA and Protein(s)

mRNA	Protein	Name
XM_008764938.2	XP_008763160.1	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X1
NM_001007706.1	NP_001007707.1	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRMT61A	NP_689520.2
	TRMT61A	XP_001083535.1
Mus musculus (house mouse)	Trmt61a	NP_001093262.1
Gallus gallus (chicken)	TRMT61A	XP_421386.4
Kluyveromyces lactis	KLLA0E15225g	XP_454636.1
Rattus norvegicus (Norway rat)	Trmt61a	NP_001007707.1
Danio rerio (zebrafish)	trmt61a	NP_001002321.1
Saccharomyces cerevisiae (baker's yeast)	GCD14	NP_012410.1
Arabidopsis thaliana (thale cress)	AT5G14600	NP_568302.1
Pan troglodytes (chimpanzee)	TRMT61A	XP_510186.3
Bos taurus (cattle)	TRMT61A	NP_001092397.1
Drosophila melanogaster (fruit fly)	CG14544	NP_651461.1
Caenorhabditis elegans (roundworm)	CELE_W02A11.1	NP_493229.1
Oryza sativa (rice)	Os04g0326300	NP_001052474.2
Canis lupus familiaris (dog)	TRMT61A	XP_547993.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003966	XP_318418.4
Eremothecium gossypii	AGOS_AFL214C	NP_985336.1
Schizosaccharomyces pombe (fission yeast)	cpd1	NP_593567.1
Xenopus tropicalis (tropical clawed frog)	trmt61a	NP_001005009.1

Gene Ontology
Bibliography

Related articles in PubMed

Base-Resolution Mapping Reveals Distinct m
Li X, Xiong X, Zhang M, Wang K, Chen Y, Zhou J, Mao Y, Lv J, Yi D, Chen XW, Wang C, Qian SB, Yi C
Molecular cell68(5)993-1005.e9(2017 Dec)

The m1A landscape on cytosolic and mitochondrial mRNA at single-base resolution.
Safra M, Sas-Chen A, Nir R, Winkler R, Nachshon A, Bar-Yaacov D, Erlacher M, Rossmanith W, Stern-Ginossar N, Schwartz S
Nature551(7679)251-255(2017 Nov)

The bipartite structure of the tRNA m1A58 methyltransferase from S. cerevisiae is conserved in humans.
Ozanick S, Krecic A, Andersland J, Anderson JT
RNA (New York, N.Y.)11(8)1281-90(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Trmt61a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trmt61a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11774	NM_001007706.1 Latest version!	Rattus norvegicus tRNA methyltransferase 61A (Trmt61a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa11774	XM_008764938.2 Latest version!	Rattus norvegicus tRNA methyltransferase 61A (Trmt61a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trmt61a ( NM_001007706.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001007707.1 Rattus norvegicus tRNA methyltransferase 61A (Trmt61a), mRNA.

Price & Availability↑

CloneID	ORa11774
Clone ID Related Accession (Same CDS sequence)	XM_008764938.2 , NM_001007706.1
Accession Version	NM_001007706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC079198.1. On Nov 29, 2004 this sequence version replaced XM_234552.2. ##Evidence-Data-START## Transcript exon combination :: BC079198.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGTTTCG TGGCATACGA GGAGCTGATC AAGGAAGGCG ACACAGCCAT CCTGTCACTG 
GGCCATGGCT CGATGGTGGC AGTGCGTGTG CAGCGTGGGG CACAGACCCA GACCCGGCAT 
GGCGTCCTGC GGCACTCAGT GGACCTCATT GGGCGTCCAT TTGGCTCCAA GGTGACTTGC 
AGCAGAGGCG GCTGGGTGTA TGTGCTGCAC CCTACTCCCG AGCTCTGGAC GGTGAACCTG 
CCCCACCGCA CACAGATCCT CTACTCTACC GACATTGCCT TAATCACCAT GATGCTGGAG 
CTGCGGCCCG GTTCTGTGGT CTGTGAATCA GGCACGGGCA GCGGTTCCGT GTCCCACGCG 
ATCATCCGCA GTATCGCCCC CACGGGCCAC CTACACACGG TAGAATTCCA CCAGCAGCGG 
GCAGACAAGG CCCGGGAGGA GTTCCAGCAG CATCGGGTGA GCCAGTGGGT GACTGTGCAC 
ACTCAGGATG TGTGCCGGAG TGGCTTCGGT GTGGTTCACG TGGCTGACGC CGTCTTCTTG 
GATATCCCAT CACCCTGGGA AGCTGTGGGC CATGCCTGGG ATGCCCTCAA GGTTGAAGGT 
GGGCGCTTCT GCTCCTTCTC GCCGTGCATT GAACAGGTGC AGCGCACATG CCAGGCCCTG 
GCAGCCCATG GCTTCACAGA GCTCAGCACC CTGGAGGTGC TGCCACAGGT CTACAATGTG 
CGCACTGTCA GTCTGCCCTT GCCTGACCTG GGGGCCGACG ACTTAGAGGG CAACGTGGGC 
TCTGATGCCA CCCCCTTCCG TAGTGGCACA CCCATGAAGG AGACTGTGGG CCACACTGGC 
TACCTCACTT TTGCCACCAA GACCCCAGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007707.1
CDS	64..936
Translation	MSFVAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCSRGGWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIRSIAPTGHLHTVEFHQQRADKAREEFQQHRVSQWVTVHTQDVCRSGFGVVHVADAVFLDIPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVRTVSLPLPDLGADDLEGNVGSDATPFRSGTPMKETVGHTGYLTFATKTPG

Target ORF information:

RefSeq Version	NM_001007706.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tRNA methyltransferase 61A (Trmt61a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007706.1

ORF Insert Sequence:

ATGAGTTTCG TGGCATACGA GGAGCTGATC AAGGAAGGCG ACACAGCCAT CCTGTCACTG 
GGCCATGGCT CGATGGTGGC AGTGCGTGTG CAGCGTGGGG CACAGACCCA GACCCGGCAT 
GGCGTCCTGC GGCACTCAGT GGACCTCATT GGGCGTCCAT TTGGCTCCAA GGTGACTTGC 
AGCAGAGGCG GCTGGGTGTA TGTGCTGCAC CCTACTCCCG AGCTCTGGAC GGTGAACCTG 
CCCCACCGCA CACAGATCCT CTACTCTACC GACATTGCCT TAATCACCAT GATGCTGGAG 
CTGCGGCCCG GTTCTGTGGT CTGTGAATCA GGCACGGGCA GCGGTTCCGT GTCCCACGCG 
ATCATCCGCA GTATCGCCCC CACGGGCCAC CTACACACGG TAGAATTCCA CCAGCAGCGG 
GCAGACAAGG CCCGGGAGGA GTTCCAGCAG CATCGGGTGA GCCAGTGGGT GACTGTGCAC 
ACTCAGGATG TGTGCCGGAG TGGCTTCGGT GTGGTTCACG TGGCTGACGC CGTCTTCTTG 
GATATCCCAT CACCCTGGGA AGCTGTGGGC CATGCCTGGG ATGCCCTCAA GGTTGAAGGT 
GGGCGCTTCT GCTCCTTCTC GCCGTGCATT GAACAGGTGC AGCGCACATG CCAGGCCCTG 
GCAGCCCATG GCTTCACAGA GCTCAGCACC CTGGAGGTGC TGCCACAGGT CTACAATGTG 
CGCACTGTCA GTCTGCCCTT GCCTGACCTG GGGGCCGACG ACTTAGAGGG CAACGTGGGC 
TCTGATGCCA CCCCCTTCCG TAGTGGCACA CCCATGAAGG AGACTGTGGG CCACACTGGC 
TACCTCACTT TTGCCACCAA GACCCCAGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trmt61a ( XM_008764938.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763160.1 Rattus norvegicus tRNA methyltransferase 61A (Trmt61a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa11774
Clone ID Related Accession (Same CDS sequence)	XM_008764938.2 , NM_001007706.1
Accession Version	XM_008764938.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764938.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTCG TGGCATACGA GGAGCTGATC AAGGAAGGCG ACACAGCCAT CCTGTCACTG 
GGCCATGGCT CGATGGTGGC AGTGCGTGTG CAGCGTGGGG CACAGACCCA GACCCGGCAT 
GGCGTCCTGC GGCACTCAGT GGACCTCATT GGGCGTCCAT TTGGCTCCAA GGTGACTTGC 
AGCAGAGGCG GCTGGGTGTA TGTGCTGCAC CCTACTCCCG AGCTCTGGAC GGTGAACCTG 
CCCCACCGCA CACAGATCCT CTACTCTACC GACATTGCCT TAATCACCAT GATGCTGGAG 
CTGCGGCCCG GTTCTGTGGT CTGTGAATCA GGCACGGGCA GCGGTTCCGT GTCCCACGCG 
ATCATCCGCA GTATCGCCCC CACGGGCCAC CTACACACGG TAGAATTCCA CCAGCAGCGG 
GCAGACAAGG CCCGGGAGGA GTTCCAGCAG CATCGGGTGA GCCAGTGGGT GACTGTGCAC 
ACTCAGGATG TGTGCCGGAG TGGCTTCGGT GTGGTTCACG TGGCTGACGC CGTCTTCTTG 
GATATCCCAT CACCCTGGGA AGCTGTGGGC CATGCCTGGG ATGCCCTCAA GGTTGAAGGT 
GGGCGCTTCT GCTCCTTCTC GCCGTGCATT GAACAGGTGC AGCGCACATG CCAGGCCCTG 
GCAGCCCATG GCTTCACAGA GCTCAGCACC CTGGAGGTGC TGCCACAGGT CTACAATGTG 
CGCACTGTCA GTCTGCCCTT GCCTGACCTG GGGGCCGACG ACTTAGAGGG CAACGTGGGC 
TCTGATGCCA CCCCCTTCCG TAGTGGCACA CCCATGAAGG AGACTGTGGG CCACACTGGC 
TACCTCACTT TTGCCACCAA GACCCCAGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763160.1
CDS	549..1421
Translation	MSFVAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCSRGGWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIRSIAPTGHLHTVEFHQQRADKAREEFQQHRVSQWVTVHTQDVCRSGFGVVHVADAVFLDIPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVRTVSLPLPDLGADDLEGNVGSDATPFRSGTPMKETVGHTGYLTFATKTPG

Target ORF information:

RefSeq Version	XM_008764938.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tRNA methyltransferase 61A (Trmt61a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764938.2

ORF Insert Sequence:

ATGAGTTTCG TGGCATACGA GGAGCTGATC AAGGAAGGCG ACACAGCCAT CCTGTCACTG 
GGCCATGGCT CGATGGTGGC AGTGCGTGTG CAGCGTGGGG CACAGACCCA GACCCGGCAT 
GGCGTCCTGC GGCACTCAGT GGACCTCATT GGGCGTCCAT TTGGCTCCAA GGTGACTTGC 
AGCAGAGGCG GCTGGGTGTA TGTGCTGCAC CCTACTCCCG AGCTCTGGAC GGTGAACCTG 
CCCCACCGCA CACAGATCCT CTACTCTACC GACATTGCCT TAATCACCAT GATGCTGGAG 
CTGCGGCCCG GTTCTGTGGT CTGTGAATCA GGCACGGGCA GCGGTTCCGT GTCCCACGCG 
ATCATCCGCA GTATCGCCCC CACGGGCCAC CTACACACGG TAGAATTCCA CCAGCAGCGG 
GCAGACAAGG CCCGGGAGGA GTTCCAGCAG CATCGGGTGA GCCAGTGGGT GACTGTGCAC 
ACTCAGGATG TGTGCCGGAG TGGCTTCGGT GTGGTTCACG TGGCTGACGC CGTCTTCTTG 
GATATCCCAT CACCCTGGGA AGCTGTGGGC CATGCCTGGG ATGCCCTCAA GGTTGAAGGT 
GGGCGCTTCT GCTCCTTCTC GCCGTGCATT GAACAGGTGC AGCGCACATG CCAGGCCCTG 
GCAGCCCATG GCTTCACAGA GCTCAGCACC CTGGAGGTGC TGCCACAGGT CTACAATGTG 
CGCACTGTCA GTCTGCCCTT GCCTGACCTG GGGGCCGACG ACTTAGAGGG CAACGTGGGC 
TCTGATGCCA CCCCCTTCCG TAGTGGCACA CCCATGAAGG AGACTGTGGG CCACACTGGC 
TACCTCACTT TTGCCACCAA GACCCCAGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Base-Resolution Mapping Reveals Distinct m
Molecular cell68(5)993-1005.e9(2017 Dec)
Li X,Xiong X,Zhang M,Wang K,Chen Y,Zhou J,Mao Y,Lv J,Yi D,Chen XW,Wang C,Qian SB,Yi C

The m1A landscape on cytosolic and mitochondrial mRNA at single-base resolution.
Nature551(7679)251-255(2017 Nov)
Safra M,Sas-Chen A,Nir R,Winkler R,Nachshon A,Bar-Yaacov D,Erlacher M,Rossmanith W,Stern-Ginossar N,Schwartz S

The bipartite structure of the tRNA m1A58 methyltransferase from S. cerevisiae is conserved in humans.
RNA (New York, N.Y.)11(8)1281-90(2005 Aug)
Ozanick S,Krecic A,Andersland J,Anderson JT

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Trmt61a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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