DENND4A gene cDNA ORF clone, Equus caballus(horse)

mRNA	Protein	Name
XM_014733813.1	XP_014589299.1	C-myc promoter-binding protein isoform X5
XM_023655089.1	XP_023510857.1	C-myc promoter-binding protein isoform X1
XM_023655064.1	XP_023510832.1	C-myc promoter-binding protein isoform X1
XM_023655147.1	XP_023510915.1	C-myc promoter-binding protein isoform X8
XM_014733812.1	XP_014589298.1	C-myc promoter-binding protein isoform X4
XM_014733808.1	XP_014589294.1	C-myc promoter-binding protein isoform X1
XM_014733809.1	XP_014589295.1	C-myc promoter-binding protein isoform X1
XM_014733811.1	XP_014589297.1	C-myc promoter-binding protein isoform X3
XM_023655081.1	XP_023510849.1	C-myc promoter-binding protein isoform X1
XM_023655111.1	XP_023510879.1	C-myc promoter-binding protein isoform X4
XM_005602983.2	XP_005603040.1	C-myc promoter-binding protein isoform X2
XM_023655095.1	XP_023510863.1	C-myc promoter-binding protein isoform X2
XM_023655118.1	XP_023510886.1	C-myc promoter-binding protein isoform X5
XM_023655127.1	XP_023510895.1	C-myc promoter-binding protein isoform X6
XM_023655154.1	XP_023510922.1	C-myc promoter-binding protein isoform X8
XM_014733810.1	XP_014589296.1	C-myc promoter-binding protein isoform X1
XM_023655074.1	XP_023510842.1	C-myc promoter-binding protein isoform X1
XM_023655106.1	XP_023510874.1	C-myc promoter-binding protein isoform X3
XM_023655138.1	XP_023510906.1	C-myc promoter-binding protein isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHb28792	XM_014733808.1 Latest version!	Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OHb28792	XM_023655106.1 Latest version!	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OHb28792	XM_014733809.1 Latest version!	Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OHb28792	XM_014733810.1 Latest version!	Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OHb28793	XM_014733812.1 Latest version!	Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OHb28794	XM_014733813.1 Latest version!	Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OHb41920	XM_023655064.1 Latest version!	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHb41920	XM_023655089.1 Latest version!	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHb41920	XM_023655081.1 Latest version!	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHb41920	XM_023655074.1 Latest version!	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHb41921	XM_023655095.1 Latest version!	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHb41922	XM_023655118.1 Latest version!	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHb41923	XM_023655138.1 Latest version!	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHb41924	XM_023655147.1 Latest version!	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHb41924	XM_023655154.1 Latest version!	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHb10409	XM_023655111.1 Latest version!	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OHb10409	XM_005602983.2 Latest version!	Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OHb10410	XM_023655127.1 Latest version!	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OHb10410	XM_014733811.1 Latest version!	Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

DENND4A ( XM_014733808.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014589294.1 Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHb28792
Clone ID Related Accession (Same CDS sequence)	XM_014733808.1 , XM_014733809.1 , XM_014733810.1 , XM_023655106.1
Accession Version	XM_014733808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867387.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACACCCAA AAGTATCCAA TGGTCCATTT ATTGCAAAAG 
GGTGATAATT CATTTAACCA AGAACTATTG AAAAATATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAGATTTTA GAGACACTGA ATAAATGTCC TCATTTTAAA 
AGACAGAGGA GTTTATATAG AGAAATACTC TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGACA TAGATGCATT TGATAAAGAA TACAAGATGG CATACGATCG TCTCACCCCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACGGGGGT GATGGAATGT 
CGGAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014589294.1
CDS	344..6073
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVVIKSLGEEVPRDYMCIDLTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGPAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_014733808.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014733808.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACACCCAA AAGTATCCAA TGGTCCATTT ATTGCAAAAG 
GGTGATAATT CATTTAACCA AGAACTATTG AAAAATATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAGATTTTA GAGACACTGA ATAAATGTCC TCATTTTAAA 
AGACAGAGGA GTTTATATAG AGAAATACTC TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGACA TAGATGCATT TGATAAAGAA TACAAGATGG CATACGATCG TCTCACCCCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACGGGGGT GATGGAATGT 
CGGAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_014733809.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014589295.1 Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X2, mRNA.

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CloneID	OHb28792
Clone ID Related Accession (Same CDS sequence)	XM_014733808.1 , XM_014733809.1 , XM_014733810.1 , XM_023655106.1
Accession Version	XM_014733809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867387.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACACCCAA AAGTATCCAA TGGTCCATTT ATTGCAAAAG 
GGTGATAATT CATTTAACCA AGAACTATTG AAAAATATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAGATTTTA GAGACACTGA ATAAATGTCC TCATTTTAAA 
AGACAGAGGA GTTTATATAG AGAAATACTC TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGACA TAGATGCATT TGATAAAGAA TACAAGATGG CATACGATCG TCTCACCCCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACGGGGGT GATGGAATGT 
CGGAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014589295.1
CDS	340..6069
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVVIKSLGEEVPRDYMCIDLTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGPAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_014733809.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014733809.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACACCCAA AAGTATCCAA TGGTCCATTT ATTGCAAAAG 
GGTGATAATT CATTTAACCA AGAACTATTG AAAAATATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAGATTTTA GAGACACTGA ATAAATGTCC TCATTTTAAA 
AGACAGAGGA GTTTATATAG AGAAATACTC TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGACA TAGATGCATT TGATAAAGAA TACAAGATGG CATACGATCG TCTCACCCCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACGGGGGT GATGGAATGT 
CGGAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_014733810.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014589296.1 Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X3, mRNA.

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CloneID	OHb28792
Clone ID Related Accession (Same CDS sequence)	XM_014733808.1 , XM_014733809.1 , XM_014733810.1 , XM_023655106.1
Accession Version	XM_014733810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867387.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACACCCAA AAGTATCCAA TGGTCCATTT ATTGCAAAAG 
GGTGATAATT CATTTAACCA AGAACTATTG AAAAATATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAGATTTTA GAGACACTGA ATAAATGTCC TCATTTTAAA 
AGACAGAGGA GTTTATATAG AGAAATACTC TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGACA TAGATGCATT TGATAAAGAA TACAAGATGG CATACGATCG TCTCACCCCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACGGGGGT GATGGAATGT 
CGGAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014589296.1
CDS	422..6151
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVVIKSLGEEVPRDYMCIDLTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGPAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_014733810.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014733810.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACACCCAA AAGTATCCAA TGGTCCATTT ATTGCAAAAG 
GGTGATAATT CATTTAACCA AGAACTATTG AAAAATATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAGATTTTA GAGACACTGA ATAAATGTCC TCATTTTAAA 
AGACAGAGGA GTTTATATAG AGAAATACTC TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGACA TAGATGCATT TGATAAAGAA TACAAGATGG CATACGATCG TCTCACCCCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACGGGGGT GATGGAATGT 
CGGAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_023655106.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023510874.1 Equus caballus DENN domain containing 4A (DENND4A), transcript variant X6, mRNA.

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CloneID	OHb28792
Clone ID Related Accession (Same CDS sequence)	XM_014733808.1 , XM_014733809.1 , XM_014733810.1 , XM_023655106.1
Accession Version	XM_023655106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACACCCAA AAGTATCCAA TGGTCCATTT ATTGCAAAAG 
GGTGATAATT CATTTAACCA AGAACTATTG AAAAATATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAGATTTTA GAGACACTGA ATAAATGTCC TCATTTTAAA 
AGACAGAGGA GTTTATATAG AGAAATACTC TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGACA TAGATGCATT TGATAAAGAA TACAAGATGG CATACGATCG TCTCACCCCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACGGGGGT GATGGAATGT 
CGGAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023510874.1
CDS	585..6314
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVVIKSLGEEVPRDYMCIDLTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGPAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_023655106.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023655106.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACACCCAA AAGTATCCAA TGGTCCATTT ATTGCAAAAG 
GGTGATAATT CATTTAACCA AGAACTATTG AAAAATATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAGATTTTA GAGACACTGA ATAAATGTCC TCATTTTAAA 
AGACAGAGGA GTTTATATAG AGAAATACTC TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGACA TAGATGCATT TGATAAAGAA TACAAGATGG CATACGATCG TCTCACCCCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACGGGGGT GATGGAATGT 
CGGAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_014733812.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014589298.1 Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X6, mRNA.

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CloneID	OHb28793
Clone ID Related Accession (Same CDS sequence)	XM_014733812.1
Accession Version	XM_014733812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867387.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GAGTGAGTGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014589298.1
CDS	344..5746
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVVIKSLGEEVPRDYMCIDLTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGPAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFESEC

Target ORF information:

RefSeq Version	XM_014733812.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014733812.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GAGTGAGTGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_014733813.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014589299.1 Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHb28794
Clone ID Related Accession (Same CDS sequence)	XM_014733813.1
Accession Version	XM_014733813.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867387.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCAA CTATTGAGTC TTGGCCACCA AATAGCAAAT ACCCTCTCCC TGTATTTTCT 
ACATTTGTGT TAACTGGAGC CTCAGCTGAA AAGGTCTATG GTGCTGCTAT TCAGTTTTAT 
GAACCATACT CTGAGGAGAA TCTCACAGAA AAACAGAGAC TTCTTTTGGG TTTAGCTTCA 
TCGGCAGATG GGAAGTCTGA TAGTTCCAAA ACAATTCACA CTAATAAATG CATCTGTCTT 
CTTTCTCACT GGCCTTTTTT TGATGCATTC AGGAAGTTTC TGACCTTTTT GTATCGTTAC 
TCCATCTCTG GACCTCATGT CCTTCCAATT GAGAAGCATA TTTCTCATTT TATGCATAAA 
GTTCCTTTTC CATCTCCTCA GAGGCCACGG ATTTTAGTTC AGTTATCACC GCATGATAAT 
CTGATTCTCA GTCAGCCTGT TTCTTCACCT CTTCCACTAA GTGGTGGCAA GTTTTCAACA 
CTGCTTCAGA ATTTAGGCCC TGAAAATGCT GTGACACTAC TGGTGTTTGC AGTAACAGAA 
CATAAGATTC TCATCCATTC CTTACGGCCT TCCGTGCTTA CTAGTGTGAC AGAAGCATTA 
GTGTCTATGA TTTTCCCTTT TCACTGGCCA TGCCCGTATG TTCCTCTGTG CCCGCTGGCT 
TTAGCAGATG TCTTGAGTGC ACCATGTCCA TTCATAGTAG GAATTGATTC AAGATACTTT 
GATCTCTATG ACCCTCCACC AGATGTTAGT TGTGTTGACC TGGATACCAA TACCATTTCT 
CAACCTGGTG ACAAGAAAAA TGTAGCCTGG AAGATACTTC CAAAGAAACC ATGTAAAAAT 
CTGATGAACA CACTGAATAA TTTGCACCAG CAATTGGCAA AATTACAGCA GAGACCAAGA 
GAAGATGGAC TAATGGACTT AGCGATGAAT GACTATGATT TTAATTCTGG AAAGAGGTTG 
CACATGATAG ATTTGGAAAT CCAAGAGGCA TTTTTGTGTT TCATGGCCTC TATTTTAAAA 
GGTTACAGAT CATACTTAAG ACCAATAACA CAGGCCCCAT CTGAGACAGC CACAGATGCA 
ACCTCTCTTT TTGCCCTGCA AGCTTTCTTA AGAAGCCGGG ACCGATCACA TCAAAAATTC 
TATAACATGA TGACCAAAAC ACAAATGTTT ATTCGCTTCA TTGAGGAATG TTCTTTTGTC 
AGTGATAAAG ATGCAAGCCT GGCATTTTTT GATGATTGTG TAGATAAAGT GGACATGGAC 
AAGAATGGTG AAATGCGACT TATAGAACTG GATGAATCTT TCAAAAGTGA ACATACTGTC 
TTTGTAACAC CTCCTGAGAT TCCCCATCTA CCCAATGGTG AAGAACCCCC TTTACAGTAC 
AGCTATAATG GATTTCCAGT TCTTAGGAGC AGCTTATTTG AAAGACCTGA AGGGTTTCTG 
CAGACACGAA AGAGCAAATT GCCTTCAAAA CCAAGTAGTC CTGGTAGCCC TTCTCCCGTG 
TTCCGAAGAA CAAAACAGGA AATTAAATCA GCCCATAAAA TTGCCAAGAG GTATTCTTCC 
ATTCCTCAGA TGTGGTCTAG GTGTCTACTG CGTCACTGTT ATGGACTGTG GTTTATTTGT 
CTCCCAGCTT ATGTGAAAGT ATGTCATTCA AAGGTCAGGG CTCTGAAAAC GGGATATGAT 
GTGCTTAAGA AAATGCAGTC AAAGAAGATG GATCCACCCG ATGAGGTGTG CTACCGCATT 
CTTATGCAGC TCTGTGGACA GTATGATCAG CCGGTGCTTG CAGTTCGAGT GCTTTTTGAA 
ATGCAGAAAG CTGGTATTGA CCCCAATGCC ATTACTTATG GTTATTATAA TAAGGCTGTT 
TTGGAAAGTA CATGGCCTTC AAGAAGTCGT AGTGGCTATT TTCTTTGGAC AAAAGTAAGA 
AATGTTGTTT TAGGAGTGGC ACAGTTCAAA AGAGCTTTAA AGAAACATGC GCATTTATCA 
CAAACAACTC TCTCAGCAGA TGGCAGTGAC CTGGATGCTG TTAGTCATGG CAGCATGGAT 
AGCGGTCATG GGACACACAC TGTGGAGCAG GCACCTTTTA ATACGGGTTT AATCAAAGTA 
TATGCTACTG ATGATAGATC TAGTACAGGT GGCCAGTCTG ATCTTGGATA TAACTCATTA 
TCTAAGGATG AAGTTAGAAG AGGACATACA TCTACTGAAG ACATTCAAGA AGAAAAAGAT 
AGGAAAGGGA GTGATTCTAG TTCTTTGTCA GAGAGTGAGA GTGCAAAAGG AAGTGCTGAT 
TGTCTGCCTA AGCTCAGTTT TCAAAGTTCT TCCAGTATAG TTCGCCTCAG TGGTGCAAAT 
AACAACAGTG CTGATAACAC CTCAGGGAAA AGCACAGAAA GCACATCTGA GCTGCTTGTA 
ATATCATCTT TTGAGGACAC AAATGAAACA GGAAACATTC AAAGTAGATG CTTCAGGAAA 
AGACATAAAA GTGACAATGA ACCTAATTTG CAGCAGCAAA TGTCCTGGGG AAGTAGAAAC 
CGCAATCTTA GTGGAGGAGT ACTGATGGGA CTTATGCTCA ATAGGATGAA CCAGGAAGCA 
AACCCTGGTG ATATGGTTGA AAAATTAGGA GCTGATGCAA AAATTCTTTC AAATGTTATC 
TCAAAAAGCA CAAGACCAAA TAGTCTTGAC ATTGGAAAGC CACCTGTAAG ATCAAAAAGA 
GACAGTCTAG AGAAGGAATC TAGTGATGAT GATACACCTT TTGATGGTTC TAACTGTTTG 
GCAGATAAAG TGGATTCTCC CGTTATTTTT GACTTAGAAG ACTTAGACAA TGAAACAGAT 
GGATCAAAAG TGGGATATGT TGCTACACAA AATCCCAAGA GGATTCAGCG TATGAGCAGC 
AGCTTTTCAG TAAAACCTTT TGAAAAAACT GATGTTGCAA CAGGATTTGA TCCCCTCTCT 
CTTTTGGTTG CTGAGACTGA ACAGCAGCAA AAAGAGGAGG AGGAAGAGGG GGAAGATGAT 
AGCAAAAGCA TTTCAACACC ATCTGCCAGA CGTGATTTAG CTGAAGAAAT TGAGATGTAT 
ATGAATAACA TGAGCAGTCC TTTGACAAGT CGTACACCAA GCATTGATTT ACAACGGGCA 
TGTGATGATA AATTAACCAA TAAGAAAAGT CCAACATTGG TCAAGGCATG CAGAAGATCT 
AGCCTGCCTC CTAATTCTCC TAGGCCAGTG AGACTTACCA AATCTAAAAG TTACACAAAA 
AGTGAGGACA GGCCAAGGGA TAGACTCTGG TCTTCTCCAG CCTTCTCACC CACTTGCCCT 
TTTAGGGAAG AATCTCAAGA TGCATTACCC CACTCATCAC CTTCATTTAA TTTGGATACA 
CTACTAGTAC CTAAACTGGA TGTTTTAAGG AACAGCATGT TCACTGCTGG AAAAGGAGTA 
GCCGAGAAAG CAAGCAAATG GTATTCAAGG TTCACCATGT ATACTACATC ATCTAAGGAT 
CAAAGTTCTG ATCGTGCCAG TCTTTCTTCA GTGGGAGCCC AGGATTCTGA ATCTGCCTCT 
TTAACAGATG AAGATGTCTG CCACGAGCTG GAAGGAGCCG CTTCCTCCCA AGAGAGCAGT 
GCCACTTCAG GGACCAAGGG AATTGATTTC AGCAGAACAA GCCTGGGAAG TTCTGCCTCC 
TTAGAAGGAT CCCTGTCAAA GTTCGCCTTA CCTGGGAAAT CAGAAGTGGC ATCTTCCTAC 
AACACGAGTA ATACAAATAT CTTCCAGAAC TATGCAATGG AGGTTCTCAT CTCAAGTTGC 
TCTCGGTGTA GAACTTGTGA CTGCCTTGTC CATGATGAGG AAATCATGGC TGGCTGGACA 
GCAGATGACT CAAATCTCAA TACCACATGC CCGTTTTGTG GCAATCTTTT CTTGCCCTTT 
TTGAATATTG AAATAAGAGA TTTAAGACGA CCCGGAAAAT ACTTTTTGAA GTCAAGCCCA 
TCTGCAGAAA ATATGCATTT TCCGTCTTCC TTTTCAAGTC AGACAAGACA GTCTTGTATC 
TCAACATCAG CCTCTGGTCT TGACAAGTCT GTTATCTCCG TTCCAGGGAA TTTTGATCTA 
CATAGCAAAT CTAAACTGCT GGAAAATTCA TGTGCCCGAA GTATTCAGAT CCCTGCTGAT 
AGATCAAAAA CAGCTGTGTC AAAATGTCCA GTATTTCCAA TGGCCAGGAG CGTCAGTACT 
TATGGCCCCT TGGATAAAGA AGACACTGGA GGGCAGAAGC TTATTCCTAC AGGCAGCCTG 
CCAGCAACTT TACAAGGAGC TACAGATGCT TTAGGATTGG AATGGCACCT CCCAAGTCCA 
GATCCCGTCA CTGTTCCGTA TCTCAGTCCT TTGGTGGTGT GGAAGGAACT TGAAAGCTTA 
TTGGAGAATG AAGGCGATCA TGCAATAACA GTGGCAGATT TTGTGGACCA TCATCCAATT 
GTTTTTTGGA ACCTAGTGTG GTATTTTAGA CGTCTTGACT TGCCCAGTAA CTTACCTGGA 
TTGATTCTTT CTTCTGAGCA TTGTAACAAA TATTCAAAGA TTCCCCGCCA CTGTATGTCT 
GAAGATAGTA AATATGTTTT AATACAAATG TTATGGGACA ATATGAAATT ACATCAGGAT 
CCGGGTCAGC CCTTGTACAT CCTCTGGAAT GCCCACACCC AAAAGTATCC AATGGTCCAT 
TTATTGCAAA AGGGTGATAA TTCATTTAAC CAAGAACTAT TGAAAAATAT GGTAAAAAGC 
ATTAAAATGA ATGATGTCTA TGGACCAATG AGTCAGATTT TAGAGACACT GAATAAATGT 
CCTCATTTTA AAAGACAGAG GAGTTTATAT AGAGAAATAC TCTTTCTCTC ACTTGTGGCA 
CTGGGAAGAG AAAACATTGA CATAGATGCA TTTGATAAAG AATACAAGAT GGCATACGAT 
CGTCTCACCC CTAGTCAAGT CAAGAGTACA CACAACTGTG ATAGACCACC AAGTACGGGG 
GTGATGGAAT GTCGGAAAAC CTTTGGAGAA CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014589299.1
CDS	802..5823
Translation	MGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_014733813.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014733813.1

ORF Insert Sequence:

ATGGGTGCAA CTATTGAGTC TTGGCCACCA AATAGCAAAT ACCCTCTCCC TGTATTTTCT 
ACATTTGTGT TAACTGGAGC CTCAGCTGAA AAGGTCTATG GTGCTGCTAT TCAGTTTTAT 
GAACCATACT CTGAGGAGAA TCTCACAGAA AAACAGAGAC TTCTTTTGGG TTTAGCTTCA 
TCGGCAGATG GGAAGTCTGA TAGTTCCAAA ACAATTCACA CTAATAAATG CATCTGTCTT 
CTTTCTCACT GGCCTTTTTT TGATGCATTC AGGAAGTTTC TGACCTTTTT GTATCGTTAC 
TCCATCTCTG GACCTCATGT CCTTCCAATT GAGAAGCATA TTTCTCATTT TATGCATAAA 
GTTCCTTTTC CATCTCCTCA GAGGCCACGG ATTTTAGTTC AGTTATCACC GCATGATAAT 
CTGATTCTCA GTCAGCCTGT TTCTTCACCT CTTCCACTAA GTGGTGGCAA GTTTTCAACA 
CTGCTTCAGA ATTTAGGCCC TGAAAATGCT GTGACACTAC TGGTGTTTGC AGTAACAGAA 
CATAAGATTC TCATCCATTC CTTACGGCCT TCCGTGCTTA CTAGTGTGAC AGAAGCATTA 
GTGTCTATGA TTTTCCCTTT TCACTGGCCA TGCCCGTATG TTCCTCTGTG CCCGCTGGCT 
TTAGCAGATG TCTTGAGTGC ACCATGTCCA TTCATAGTAG GAATTGATTC AAGATACTTT 
GATCTCTATG ACCCTCCACC AGATGTTAGT TGTGTTGACC TGGATACCAA TACCATTTCT 
CAACCTGGTG ACAAGAAAAA TGTAGCCTGG AAGATACTTC CAAAGAAACC ATGTAAAAAT 
CTGATGAACA CACTGAATAA TTTGCACCAG CAATTGGCAA AATTACAGCA GAGACCAAGA 
GAAGATGGAC TAATGGACTT AGCGATGAAT GACTATGATT TTAATTCTGG AAAGAGGTTG 
CACATGATAG ATTTGGAAAT CCAAGAGGCA TTTTTGTGTT TCATGGCCTC TATTTTAAAA 
GGTTACAGAT CATACTTAAG ACCAATAACA CAGGCCCCAT CTGAGACAGC CACAGATGCA 
ACCTCTCTTT TTGCCCTGCA AGCTTTCTTA AGAAGCCGGG ACCGATCACA TCAAAAATTC 
TATAACATGA TGACCAAAAC ACAAATGTTT ATTCGCTTCA TTGAGGAATG TTCTTTTGTC 
AGTGATAAAG ATGCAAGCCT GGCATTTTTT GATGATTGTG TAGATAAAGT GGACATGGAC 
AAGAATGGTG AAATGCGACT TATAGAACTG GATGAATCTT TCAAAAGTGA ACATACTGTC 
TTTGTAACAC CTCCTGAGAT TCCCCATCTA CCCAATGGTG AAGAACCCCC TTTACAGTAC 
AGCTATAATG GATTTCCAGT TCTTAGGAGC AGCTTATTTG AAAGACCTGA AGGGTTTCTG 
CAGACACGAA AGAGCAAATT GCCTTCAAAA CCAAGTAGTC CTGGTAGCCC TTCTCCCGTG 
TTCCGAAGAA CAAAACAGGA AATTAAATCA GCCCATAAAA TTGCCAAGAG GTATTCTTCC 
ATTCCTCAGA TGTGGTCTAG GTGTCTACTG CGTCACTGTT ATGGACTGTG GTTTATTTGT 
CTCCCAGCTT ATGTGAAAGT ATGTCATTCA AAGGTCAGGG CTCTGAAAAC GGGATATGAT 
GTGCTTAAGA AAATGCAGTC AAAGAAGATG GATCCACCCG ATGAGGTGTG CTACCGCATT 
CTTATGCAGC TCTGTGGACA GTATGATCAG CCGGTGCTTG CAGTTCGAGT GCTTTTTGAA 
ATGCAGAAAG CTGGTATTGA CCCCAATGCC ATTACTTATG GTTATTATAA TAAGGCTGTT 
TTGGAAAGTA CATGGCCTTC AAGAAGTCGT AGTGGCTATT TTCTTTGGAC AAAAGTAAGA 
AATGTTGTTT TAGGAGTGGC ACAGTTCAAA AGAGCTTTAA AGAAACATGC GCATTTATCA 
CAAACAACTC TCTCAGCAGA TGGCAGTGAC CTGGATGCTG TTAGTCATGG CAGCATGGAT 
AGCGGTCATG GGACACACAC TGTGGAGCAG GCACCTTTTA ATACGGGTTT AATCAAAGTA 
TATGCTACTG ATGATAGATC TAGTACAGGT GGCCAGTCTG ATCTTGGATA TAACTCATTA 
TCTAAGGATG AAGTTAGAAG AGGACATACA TCTACTGAAG ACATTCAAGA AGAAAAAGAT 
AGGAAAGGGA GTGATTCTAG TTCTTTGTCA GAGAGTGAGA GTGCAAAAGG AAGTGCTGAT 
TGTCTGCCTA AGCTCAGTTT TCAAAGTTCT TCCAGTATAG TTCGCCTCAG TGGTGCAAAT 
AACAACAGTG CTGATAACAC CTCAGGGAAA AGCACAGAAA GCACATCTGA GCTGCTTGTA 
ATATCATCTT TTGAGGACAC AAATGAAACA GGAAACATTC AAAGTAGATG CTTCAGGAAA 
AGACATAAAA GTGACAATGA ACCTAATTTG CAGCAGCAAA TGTCCTGGGG AAGTAGAAAC 
CGCAATCTTA GTGGAGGAGT ACTGATGGGA CTTATGCTCA ATAGGATGAA CCAGGAAGCA 
AACCCTGGTG ATATGGTTGA AAAATTAGGA GCTGATGCAA AAATTCTTTC AAATGTTATC 
TCAAAAAGCA CAAGACCAAA TAGTCTTGAC ATTGGAAAGC CACCTGTAAG ATCAAAAAGA 
GACAGTCTAG AGAAGGAATC TAGTGATGAT GATACACCTT TTGATGGTTC TAACTGTTTG 
GCAGATAAAG TGGATTCTCC CGTTATTTTT GACTTAGAAG ACTTAGACAA TGAAACAGAT 
GGATCAAAAG TGGGATATGT TGCTACACAA AATCCCAAGA GGATTCAGCG TATGAGCAGC 
AGCTTTTCAG TAAAACCTTT TGAAAAAACT GATGTTGCAA CAGGATTTGA TCCCCTCTCT 
CTTTTGGTTG CTGAGACTGA ACAGCAGCAA AAAGAGGAGG AGGAAGAGGG GGAAGATGAT 
AGCAAAAGCA TTTCAACACC ATCTGCCAGA CGTGATTTAG CTGAAGAAAT TGAGATGTAT 
ATGAATAACA TGAGCAGTCC TTTGACAAGT CGTACACCAA GCATTGATTT ACAACGGGCA 
TGTGATGATA AATTAACCAA TAAGAAAAGT CCAACATTGG TCAAGGCATG CAGAAGATCT 
AGCCTGCCTC CTAATTCTCC TAGGCCAGTG AGACTTACCA AATCTAAAAG TTACACAAAA 
AGTGAGGACA GGCCAAGGGA TAGACTCTGG TCTTCTCCAG CCTTCTCACC CACTTGCCCT 
TTTAGGGAAG AATCTCAAGA TGCATTACCC CACTCATCAC CTTCATTTAA TTTGGATACA 
CTACTAGTAC CTAAACTGGA TGTTTTAAGG AACAGCATGT TCACTGCTGG AAAAGGAGTA 
GCCGAGAAAG CAAGCAAATG GTATTCAAGG TTCACCATGT ATACTACATC ATCTAAGGAT 
CAAAGTTCTG ATCGTGCCAG TCTTTCTTCA GTGGGAGCCC AGGATTCTGA ATCTGCCTCT 
TTAACAGATG AAGATGTCTG CCACGAGCTG GAAGGAGCCG CTTCCTCCCA AGAGAGCAGT 
GCCACTTCAG GGACCAAGGG AATTGATTTC AGCAGAACAA GCCTGGGAAG TTCTGCCTCC 
TTAGAAGGAT CCCTGTCAAA GTTCGCCTTA CCTGGGAAAT CAGAAGTGGC ATCTTCCTAC 
AACACGAGTA ATACAAATAT CTTCCAGAAC TATGCAATGG AGGTTCTCAT CTCAAGTTGC 
TCTCGGTGTA GAACTTGTGA CTGCCTTGTC CATGATGAGG AAATCATGGC TGGCTGGACA 
GCAGATGACT CAAATCTCAA TACCACATGC CCGTTTTGTG GCAATCTTTT CTTGCCCTTT 
TTGAATATTG AAATAAGAGA TTTAAGACGA CCCGGAAAAT ACTTTTTGAA GTCAAGCCCA 
TCTGCAGAAA ATATGCATTT TCCGTCTTCC TTTTCAAGTC AGACAAGACA GTCTTGTATC 
TCAACATCAG CCTCTGGTCT TGACAAGTCT GTTATCTCCG TTCCAGGGAA TTTTGATCTA 
CATAGCAAAT CTAAACTGCT GGAAAATTCA TGTGCCCGAA GTATTCAGAT CCCTGCTGAT 
AGATCAAAAA CAGCTGTGTC AAAATGTCCA GTATTTCCAA TGGCCAGGAG CGTCAGTACT 
TATGGCCCCT TGGATAAAGA AGACACTGGA GGGCAGAAGC TTATTCCTAC AGGCAGCCTG 
CCAGCAACTT TACAAGGAGC TACAGATGCT TTAGGATTGG AATGGCACCT CCCAAGTCCA 
GATCCCGTCA CTGTTCCGTA TCTCAGTCCT TTGGTGGTGT GGAAGGAACT TGAAAGCTTA 
TTGGAGAATG AAGGCGATCA TGCAATAACA GTGGCAGATT TTGTGGACCA TCATCCAATT 
GTTTTTTGGA ACCTAGTGTG GTATTTTAGA CGTCTTGACT TGCCCAGTAA CTTACCTGGA 
TTGATTCTTT CTTCTGAGCA TTGTAACAAA TATTCAAAGA TTCCCCGCCA CTGTATGTCT 
GAAGATAGTA AATATGTTTT AATACAAATG TTATGGGACA ATATGAAATT ACATCAGGAT 
CCGGGTCAGC CCTTGTACAT CCTCTGGAAT GCCCACACCC AAAAGTATCC AATGGTCCAT 
TTATTGCAAA AGGGTGATAA TTCATTTAAC CAAGAACTAT TGAAAAATAT GGTAAAAAGC 
ATTAAAATGA ATGATGTCTA TGGACCAATG AGTCAGATTT TAGAGACACT GAATAAATGT 
CCTCATTTTA AAAGACAGAG GAGTTTATAT AGAGAAATAC TCTTTCTCTC ACTTGTGGCA 
CTGGGAAGAG AAAACATTGA CATAGATGCA TTTGATAAAG AATACAAGAT GGCATACGAT 
CGTCTCACCC CTAGTCAAGT CAAGAGTACA CACAACTGTG ATAGACCACC AAGTACGGGG 
GTGATGGAAT GTCGGAAAAC CTTTGGAGAA CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_023655089.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023510857.1 Equus caballus DENN domain containing 4A (DENND4A), transcript variant X4, mRNA.

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CloneID	OHb41920
Clone ID Related Accession (Same CDS sequence)	XM_023655089.1 , XM_023655064.1 , XM_023655081.1 , XM_023655074.1
Accession Version	XM_023655089.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAGGGT GATAATTCAT TTAACCAAGA ACTATTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA GATTTTAGAG 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTCTTT 
CTCTCACTTG TGGCACTGGG AAGAGAAAAC ATTGACATAG ATGCATTTGA TAAAGAATAC 
AAGATGGCAT ACGATCGTCT CACCCCTAGT CAAGTCAAGA GTACACACAA CTGTGATAGA 
CCACCAAGTA CGGGGGTGAT GGAATGTCGG AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023510857.1
CDS	439..6195
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVVIKSLGEEVPRDYMCIDLTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGPAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_023655089.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023655089.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAGGGT GATAATTCAT TTAACCAAGA ACTATTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA GATTTTAGAG 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTCTTT 
CTCTCACTTG TGGCACTGGG AAGAGAAAAC ATTGACATAG ATGCATTTGA TAAAGAATAC 
AAGATGGCAT ACGATCGTCT CACCCCTAGT CAAGTCAAGA GTACACACAA CTGTGATAGA 
CCACCAAGTA CGGGGGTGAT GGAATGTCGG AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_023655064.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023510832.1 Equus caballus DENN domain containing 4A (DENND4A), transcript variant X1, mRNA.

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CloneID	OHb41920
Clone ID Related Accession (Same CDS sequence)	XM_023655089.1 , XM_023655064.1 , XM_023655081.1 , XM_023655074.1
Accession Version	XM_023655064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAGGGT GATAATTCAT TTAACCAAGA ACTATTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA GATTTTAGAG 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTCTTT 
CTCTCACTTG TGGCACTGGG AAGAGAAAAC ATTGACATAG ATGCATTTGA TAAAGAATAC 
AAGATGGCAT ACGATCGTCT CACCCCTAGT CAAGTCAAGA GTACACACAA CTGTGATAGA 
CCACCAAGTA CGGGGGTGAT GGAATGTCGG AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023510832.1
CDS	586..6342
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVVIKSLGEEVPRDYMCIDLTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGPAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_023655064.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023655064.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAGGGT GATAATTCAT TTAACCAAGA ACTATTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA GATTTTAGAG 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTCTTT 
CTCTCACTTG TGGCACTGGG AAGAGAAAAC ATTGACATAG ATGCATTTGA TAAAGAATAC 
AAGATGGCAT ACGATCGTCT CACCCCTAGT CAAGTCAAGA GTACACACAA CTGTGATAGA 
CCACCAAGTA CGGGGGTGAT GGAATGTCGG AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_023655081.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023510849.1 Equus caballus DENN domain containing 4A (DENND4A), transcript variant X3, mRNA.

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CloneID	OHb41920
Clone ID Related Accession (Same CDS sequence)	XM_023655089.1 , XM_023655064.1 , XM_023655081.1 , XM_023655074.1
Accession Version	XM_023655081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAGGGT GATAATTCAT TTAACCAAGA ACTATTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA GATTTTAGAG 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTCTTT 
CTCTCACTTG TGGCACTGGG AAGAGAAAAC ATTGACATAG ATGCATTTGA TAAAGAATAC 
AAGATGGCAT ACGATCGTCT CACCCCTAGT CAAGTCAAGA GTACACACAA CTGTGATAGA 
CCACCAAGTA CGGGGGTGAT GGAATGTCGG AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023510849.1
CDS	582..6338
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVVIKSLGEEVPRDYMCIDLTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGPAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_023655081.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023655081.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAGGGT GATAATTCAT TTAACCAAGA ACTATTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA GATTTTAGAG 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTCTTT 
CTCTCACTTG TGGCACTGGG AAGAGAAAAC ATTGACATAG ATGCATTTGA TAAAGAATAC 
AAGATGGCAT ACGATCGTCT CACCCCTAGT CAAGTCAAGA GTACACACAA CTGTGATAGA 
CCACCAAGTA CGGGGGTGAT GGAATGTCGG AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_023655074.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023510842.1 Equus caballus DENN domain containing 4A (DENND4A), transcript variant X2, mRNA.

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CloneID	OHb41920
Clone ID Related Accession (Same CDS sequence)	XM_023655089.1 , XM_023655064.1 , XM_023655081.1 , XM_023655074.1
Accession Version	XM_023655074.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAGGGT GATAATTCAT TTAACCAAGA ACTATTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA GATTTTAGAG 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTCTTT 
CTCTCACTTG TGGCACTGGG AAGAGAAAAC ATTGACATAG ATGCATTTGA TAAAGAATAC 
AAGATGGCAT ACGATCGTCT CACCCCTAGT CAAGTCAAGA GTACACACAA CTGTGATAGA 
CCACCAAGTA CGGGGGTGAT GGAATGTCGG AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023510842.1
CDS	571..6327
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVVIKSLGEEVPRDYMCIDLTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGPAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_023655074.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023655074.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG CGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ACTCATTATC TAAGGATGAA 
GTTAGAAGAG GACATACATC TACTGAAGAC ATTCAAGAAG AAAAAGATAG GAAAGGGAGT 
GATTCTAGTT CTTTGTCAGA GAGTGAGAGT GCAAAAGGAA GTGCTGATTG TCTGCCTAAG 
CTCAGTTTTC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTGCAAATAA CAACAGTGCT 
GATAACACCT CAGGGAAAAG CACAGAAAGC ACATCTGAGC TGCTTGTAAT ATCATCTTTT 
GAGGACACAA ATGAAACAGG AAACATTCAA AGTAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAATGAAC CTAATTTGCA GCAGCAAATG TCCTGGGGAA GTAGAAACCG CAATCTTAGT 
GGAGGAGTAC TGATGGGACT TATGCTCAAT AGGATGAACC AGGAAGCAAA CCCTGGTGAT 
ATGGTTGAAA AATTAGGAGC TGATGCAAAA ATTCTTTCAA ATGTTATCTC AAAAAGCACA 
AGACCAAATA GTCTTGACAT TGGAAAGCCA CCTGTAAGAT CAAAAAGAGA CAGTCTAGAG 
AAGGAATCTA GTGATGATGA TACACCTTTT GATGGTTCTA ACTGTTTGGC AGATAAAGTG 
GATTCTCCCG TTATTTTTGA CTTAGAAGAC TTAGACAATG AAACAGATGG ATCAAAAGTG 
GGATATGTTG CTACACAAAA TCCCAAGAGG ATTCAGCGTA TGAGCAGCAG CTTTTCAGTA 
AAACCTTTTG AAAAAACTGA TGTTGCAACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAGGAG GAAGAGGGGG AAGATGATAG CAAAAGCATT 
TCAACACCAT CTGCCAGACG TGATTTAGCT GAAGAAATTG AGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGTCG TACACCAAGC ATTGATTTAC AACGGGCATG TGATGATAAA 
TTAACCAATA AGAAAAGTCC AACATTGGTC AAGGCATGCA GAAGATCTAG CCTGCCTCCT 
AATTCTCCTA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGACAGG 
CCAAGGGATA GACTCTGGTC TTCTCCAGCC TTCTCACCCA CTTGCCCTTT TAGGGAAGAA 
TCTCAAGATG CATTACCCCA CTCATCACCT TCATTTAATT TGGATACACT ACTAGTACCT 
AAACTGGATG TTTTAAGGAA CAGCATGTTC ACTGCTGGAA AAGGAGTAGC CGAGAAAGCA 
AGCAAATGGT ATTCAAGGTT CACCATGTAT ACTACATCAT CTAAGGATCA AAGTTCTGAT 
CGTGCCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTGCCTCTTT AACAGATGAA 
GATGTCTGCC ACGAGCTGGA AGGAGCCGCT TCCTCCCAAG AGAGCAGTGC CACTTCAGGG 
ACCAAGGGAA TTGATTTCAG CAGAACAAGC CTGGGAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TCGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTACAA CACGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGACT GCCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGACTCA 
AATCTCAATA CCACATGCCC GTTTTGTGGC AATCTTTTCT TGCCCTTTTT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAAATAC TTTTTGAAGT CAAGCCCATC TGCAGAAAAT 
ATGCATTTTC CGTCTTCCTT TTCAAGTCAG ACAAGACAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACAAGTCTGT TATCTCCGTT CCAGGGAATT TTGATCTACA TAGCAAATCT 
AAACTGCTGG AAAATTCATG TGCCCGAAGT ATTCAGATCC CTGCTGATAG ATCAAAAACA 
GCTGTGTCAA AATGTCCAGT ATTTCCAATG GCCAGGAGCG TCAGTACTTA TGGCCCCTTG 
GATAAAGAAG ACACTGGAGG GCAGAAGCTT ATTCCTACAG GCAGCCTGCC AGCAACTTTA 
CAAGGAGCTA CAGATGCTTT AGGATTGGAA TGGCACCTCC CAAGTCCAGA TCCCGTCACT 
GTTCCGTATC TCAGTCCTTT GGTGGTGTGG AAGGAACTTG AAAGCTTATT GGAGAATGAA 
GGCGATCATG CAATAACAGT GGCAGATTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTAGTGTGGT ATTTTAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCATT GTAACAAATA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGTAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGTCAGCCC 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAGGGT GATAATTCAT TTAACCAAGA ACTATTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA GATTTTAGAG 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTCTTT 
CTCTCACTTG TGGCACTGGG AAGAGAAAAC ATTGACATAG ATGCATTTGA TAAAGAATAC 
AAGATGGCAT ACGATCGTCT CACCCCTAGT CAAGTCAAGA GTACACACAA CTGTGATAGA 
CCACCAAGTA CGGGGGTGAT GGAATGTCGG AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_023655095.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023510863.1 Equus caballus DENN domain containing 4A (DENND4A), transcript variant X5, mRNA.

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CloneID	OHb41921
Clone ID Related Accession (Same CDS sequence)	XM_023655095.1
Accession Version	XM_023655095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGCGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAACT CATTATCTAA GGATGAAGTT 
AGAAGAGGAC ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAGGAA AGGGAGTGAT 
TCTAGTTCTT TGTCAGAGAG TGAGAGTGCA AAAGGAAGTG CTGATTGTCT GCCTAAGCTC 
AGTTTTCAAA GTTCTTCCAG TATAGTTCGC CTCAGTGGTG CAAATAACAA CAGTGCTGAT 
AACACCTCAG GGAAAAGCAC AGAAAGCACA TCTGAGCTGC TTGTAATATC ATCTTTTGAG 
GACACAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAACCTA ATTTGCAGCA GCAAATGTCC TGGGGAAGTA GAAACCGCAA TCTTAGTGGA 
GGAGTACTGA TGGGACTTAT GCTCAATAGG ATGAACCAGG AAGCAAACCC TGGTGATATG 
GTTGAAAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACAAGA 
CCAAATAGTC TTGACATTGG AAAGCCACCT GTAAGATCAA AAAGAGACAG TCTAGAGAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT GTTTGGCAGA TAAAGTGGAT 
TCTCCCGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATGGATC AAAAGTGGGA 
TATGTTGCTA CACAAAATCC CAAGAGGATT CAGCGTATGA GCAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT TGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGGGGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CCAGACGTGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
ACCAATAAGA AAAGTCCAAC ATTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCTAGGC CAGTGAGACT TACCAAATCT AAAAGTTACA CAAAAAGTGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCCACTT GCCCTTTTAG GGAAGAATCT 
CAAGATGCAT TACCCCACTC ATCACCTTCA TTTAATTTGG ATACACTACT AGTACCTAAA 
CTGGATGTTT TAAGGAACAG CATGTTCACT GCTGGAAAAG GAGTAGCCGA GAAAGCAAGC 
AAATGGTATT CAAGGTTCAC CATGTATACT ACATCATCTA AGGATCAAAG TTCTGATCGT 
GCCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTG CCTCTTTAAC AGATGAAGAT 
GTCTGCCACG AGCTGGAAGG AGCCGCTTCC TCCCAAGAGA GCAGTGCCAC TTCAGGGACC 
AAGGGAATTG ATTTCAGCAG AACAAGCCTG GGAAGTTCTG CCTCCTTAGA AGGATCCCTG 
TCAAAGTTCG CCTTACCTGG GAAATCAGAA GTGGCATCTT CCTACAACAC GAGTAATACA 
AATATCTTCC AGAACTATGC AATGGAGGTT CTCATCTCAA GTTGCTCTCG GTGTAGAACT 
TGTGACTGCC TTGTCCATGA TGAGGAAATC ATGGCTGGCT GGACAGCAGA TGACTCAAAT 
CTCAATACCA CATGCCCGTT TTGTGGCAAT CTTTTCTTGC CCTTTTTGAA TATTGAAATA 
AGAGATTTAA GACGACCCGG AAAATACTTT TTGAAGTCAA GCCCATCTGC AGAAAATATG 
CATTTTCCGT CTTCCTTTTC AAGTCAGACA AGACAGTCTT GTATCTCAAC ATCAGCCTCT 
GGTCTTGACA AGTCTGTTAT CTCCGTTCCA GGGAATTTTG ATCTACATAG CAAATCTAAA 
CTGCTGGAAA ATTCATGTGC CCGAAGTATT CAGATCCCTG CTGATAGATC AAAAACAGCT 
GTGTCAAAAT GTCCAGTATT TCCAATGGCC AGGAGCGTCA GTACTTATGG CCCCTTGGAT 
AAAGAAGACA CTGGAGGGCA GAAGCTTATT CCTACAGGCA GCCTGCCAGC AACTTTACAA 
GGAGCTACAG ATGCTTTAGG ATTGGAATGG CACCTCCCAA GTCCAGATCC CGTCACTGTT 
CCGTATCTCA GTCCTTTGGT GGTGTGGAAG GAACTTGAAA GCTTATTGGA GAATGAAGGC 
GATCATGCAA TAACAGTGGC AGATTTTGTG GACCATCATC CAATTGTTTT TTGGAACCTA 
GTGTGGTATT TTAGACGTCT TGACTTGCCC AGTAACTTAC CTGGATTGAT TCTTTCTTCT 
GAGCATTGTA ACAAATATTC AAAGATTCCC CGCCACTGTA TGTCTGAAGA TAGTAAATAT 
GTTTTAATAC AAATGTTATG GGACAATATG AAATTACATC AGGATCCGGG TCAGCCCTTG 
TACATCCTCT GGAATGCCCA CAGTCAAAAT CGTACTCTTT TGTTTGAGAC CCAAAAGTAT 
CCAATGGTCC ATTTATTGCA AAAGGGTGAT AATTCATTTA ACCAAGAACT ATTGAAAAAT 
ATGGTAAAAA GCATTAAAAT GAATGATGTC TATGGACCAA TGAGTCAGAT TTTAGAGACA 
CTGAATAAAT GTCCTCATTT TAAAAGACAG AGGAGTTTAT ATAGAGAAAT ACTCTTTCTC 
TCACTTGTGG CACTGGGAAG AGAAAACATT GACATAGATG CATTTGATAA AGAATACAAG 
ATGGCATACG ATCGTCTCAC CCCTAGTCAA GTCAAGAGTA CACACAACTG TGATAGACCA 
CCAAGTACGG GGGTGATGGA ATGTCGGAAA ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023510863.1
CDS	585..6338
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVVIKSLGEEVPRDYMCIDLTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGPAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSDGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_023655095.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023655095.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGCGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAACT CATTATCTAA GGATGAAGTT 
AGAAGAGGAC ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAGGAA AGGGAGTGAT 
TCTAGTTCTT TGTCAGAGAG TGAGAGTGCA AAAGGAAGTG CTGATTGTCT GCCTAAGCTC 
AGTTTTCAAA GTTCTTCCAG TATAGTTCGC CTCAGTGGTG CAAATAACAA CAGTGCTGAT 
AACACCTCAG GGAAAAGCAC AGAAAGCACA TCTGAGCTGC TTGTAATATC ATCTTTTGAG 
GACACAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAACCTA ATTTGCAGCA GCAAATGTCC TGGGGAAGTA GAAACCGCAA TCTTAGTGGA 
GGAGTACTGA TGGGACTTAT GCTCAATAGG ATGAACCAGG AAGCAAACCC TGGTGATATG 
GTTGAAAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACAAGA 
CCAAATAGTC TTGACATTGG AAAGCCACCT GTAAGATCAA AAAGAGACAG TCTAGAGAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT GTTTGGCAGA TAAAGTGGAT 
TCTCCCGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATGGATC AAAAGTGGGA 
TATGTTGCTA CACAAAATCC CAAGAGGATT CAGCGTATGA GCAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT TGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGGGGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CCAGACGTGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
ACCAATAAGA AAAGTCCAAC ATTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCTAGGC CAGTGAGACT TACCAAATCT AAAAGTTACA CAAAAAGTGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCCACTT GCCCTTTTAG GGAAGAATCT 
CAAGATGCAT TACCCCACTC ATCACCTTCA TTTAATTTGG ATACACTACT AGTACCTAAA 
CTGGATGTTT TAAGGAACAG CATGTTCACT GCTGGAAAAG GAGTAGCCGA GAAAGCAAGC 
AAATGGTATT CAAGGTTCAC CATGTATACT ACATCATCTA AGGATCAAAG TTCTGATCGT 
GCCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTG CCTCTTTAAC AGATGAAGAT 
GTCTGCCACG AGCTGGAAGG AGCCGCTTCC TCCCAAGAGA GCAGTGCCAC TTCAGGGACC 
AAGGGAATTG ATTTCAGCAG AACAAGCCTG GGAAGTTCTG CCTCCTTAGA AGGATCCCTG 
TCAAAGTTCG CCTTACCTGG GAAATCAGAA GTGGCATCTT CCTACAACAC GAGTAATACA 
AATATCTTCC AGAACTATGC AATGGAGGTT CTCATCTCAA GTTGCTCTCG GTGTAGAACT 
TGTGACTGCC TTGTCCATGA TGAGGAAATC ATGGCTGGCT GGACAGCAGA TGACTCAAAT 
CTCAATACCA CATGCCCGTT TTGTGGCAAT CTTTTCTTGC CCTTTTTGAA TATTGAAATA 
AGAGATTTAA GACGACCCGG AAAATACTTT TTGAAGTCAA GCCCATCTGC AGAAAATATG 
CATTTTCCGT CTTCCTTTTC AAGTCAGACA AGACAGTCTT GTATCTCAAC ATCAGCCTCT 
GGTCTTGACA AGTCTGTTAT CTCCGTTCCA GGGAATTTTG ATCTACATAG CAAATCTAAA 
CTGCTGGAAA ATTCATGTGC CCGAAGTATT CAGATCCCTG CTGATAGATC AAAAACAGCT 
GTGTCAAAAT GTCCAGTATT TCCAATGGCC AGGAGCGTCA GTACTTATGG CCCCTTGGAT 
AAAGAAGACA CTGGAGGGCA GAAGCTTATT CCTACAGGCA GCCTGCCAGC AACTTTACAA 
GGAGCTACAG ATGCTTTAGG ATTGGAATGG CACCTCCCAA GTCCAGATCC CGTCACTGTT 
CCGTATCTCA GTCCTTTGGT GGTGTGGAAG GAACTTGAAA GCTTATTGGA GAATGAAGGC 
GATCATGCAA TAACAGTGGC AGATTTTGTG GACCATCATC CAATTGTTTT TTGGAACCTA 
GTGTGGTATT TTAGACGTCT TGACTTGCCC AGTAACTTAC CTGGATTGAT TCTTTCTTCT 
GAGCATTGTA ACAAATATTC AAAGATTCCC CGCCACTGTA TGTCTGAAGA TAGTAAATAT 
GTTTTAATAC AAATGTTATG GGACAATATG AAATTACATC AGGATCCGGG TCAGCCCTTG 
TACATCCTCT GGAATGCCCA CAGTCAAAAT CGTACTCTTT TGTTTGAGAC CCAAAAGTAT 
CCAATGGTCC ATTTATTGCA AAAGGGTGAT AATTCATTTA ACCAAGAACT ATTGAAAAAT 
ATGGTAAAAA GCATTAAAAT GAATGATGTC TATGGACCAA TGAGTCAGAT TTTAGAGACA 
CTGAATAAAT GTCCTCATTT TAAAAGACAG AGGAGTTTAT ATAGAGAAAT ACTCTTTCTC 
TCACTTGTGG CACTGGGAAG AGAAAACATT GACATAGATG CATTTGATAA AGAATACAAG 
ATGGCATACG ATCGTCTCAC CCCTAGTCAA GTCAAGAGTA CACACAACTG TGATAGACCA 
CCAAGTACGG GGGTGATGGA ATGTCGGAAA ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_023655118.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023510886.1 Equus caballus DENN domain containing 4A (DENND4A), transcript variant X8, mRNA.

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CloneID	OHb41922
Clone ID Related Accession (Same CDS sequence)	XM_023655118.1
Accession Version	XM_023655118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGGTGGCC AGTCTGATCT TGGATATAAC TCATTATCTA AGGATGAAGT TAGAAGAGGA 
CATACATCTA CTGAAGACAT TCAAGAAGAA AAAGATAGGA AAGGGAGTGA TTCTAGTTCT 
TTGTCAGAGA GTGAGAGTGC AAAAGGAAGT GCTGATTGTC TGCCTAAGCT CAGTTTTCAA 
AGTTCTTCCA GTATAGTTCG CCTCAGTGGT GCAAATAACA ACAGTGCTGA TAACACCTCA 
GGGAAAAGCA CAGAAAGCAC ATCTGAGCTG CTTGTAATAT CATCTTTTGA GGACACAAAT 
GAAACAGGAA ACATTCAAAG TAGATGCTTC AGGAAAAGAC ATAAAAGTGA CAATGAACCT 
AATTTGCAGC AGCAAATGTC CTGGGGAAGT AGAAACCGCA ATCTTAGTGG AGGAGTACTG 
ATGGGACTTA TGCTCAATAG GATGAACCAG GAAGCAAACC CTGGTGATAT GGTTGAAAAA 
TTAGGAGCTG ATGCAAAAAT TCTTTCAAAT GTTATCTCAA AAAGCACAAG ACCAAATAGT 
CTTGACATTG GAAAGCCACC TGTAAGATCA AAAAGAGACA GTCTAGAGAA GGAATCTAGT 
GATGATGATA CACCTTTTGA TGGTTCTAAC TGTTTGGCAG ATAAAGTGGA TTCTCCCGTT 
ATTTTTGACT TAGAAGACTT AGACAATGAA ACAGATGGAT CAAAAGTGGG ATATGTTGCT 
ACACAAAATC CCAAGAGGAT TCAGCGTATG AGCAGCAGCT TTTCAGTAAA ACCTTTTGAA 
AAAACTGATG TTGCAACAGG ATTTGATCCC CTCTCTCTTT TGGTTGCTGA GACTGAACAG 
CAGCAAAAAG AGGAGGAGGA AGAGGGGGAA GATGATAGCA AAAGCATTTC AACACCATCT 
GCCAGACGTG ATTTAGCTGA AGAAATTGAG ATGTATATGA ATAACATGAG CAGTCCTTTG 
ACAAGTCGTA CACCAAGCAT TGATTTACAA CGGGCATGTG ATGATAAATT AACCAATAAG 
AAAAGTCCAA CATTGGTCAA GGCATGCAGA AGATCTAGCC TGCCTCCTAA TTCTCCTAGG 
CCAGTGAGAC TTACCAAATC TAAAAGTTAC ACAAAAAGTG AGGACAGGCC AAGGGATAGA 
CTCTGGTCTT CTCCAGCCTT CTCACCCACT TGCCCTTTTA GGGAAGAATC TCAAGATGCA 
TTACCCCACT CATCACCTTC ATTTAATTTG GATACACTAC TAGTACCTAA ACTGGATGTT 
TTAAGGAACA GCATGTTCAC TGCTGGAAAA GGAGTAGCCG AGAAAGCAAG CAAATGGTAT 
TCAAGGTTCA CCATGTATAC TACATCATCT AAGGATCAAA GTTCTGATCG TGCCAGTCTT 
TCTTCAGTGG GAGCCCAGGA TTCTGAATCT GCCTCTTTAA CAGATGAAGA TGTCTGCCAC 
GAGCTGGAAG GAGCCGCTTC CTCCCAAGAG AGCAGTGCCA CTTCAGGGAC CAAGGGAATT 
GATTTCAGCA GAACAAGCCT GGGAAGTTCT GCCTCCTTAG AAGGATCCCT GTCAAAGTTC 
GCCTTACCTG GGAAATCAGA AGTGGCATCT TCCTACAACA CGAGTAATAC AAATATCTTC 
CAGAACTATG CAATGGAGGT TCTCATCTCA AGTTGCTCTC GGTGTAGAAC TTGTGACTGC 
CTTGTCCATG ATGAGGAAAT CATGGCTGGC TGGACAGCAG ATGACTCAAA TCTCAATACC 
ACATGCCCGT TTTGTGGCAA TCTTTTCTTG CCCTTTTTGA ATATTGAAAT AAGAGATTTA 
AGACGACCCG GAAAATACTT TTTGAAGTCA AGCCCATCTG CAGAAAATAT GCATTTTCCG 
TCTTCCTTTT CAAGTCAGAC AAGACAGTCT TGTATCTCAA CATCAGCCTC TGGTCTTGAC 
AAGTCTGTTA TCTCCGTTCC AGGGAATTTT GATCTACATA GCAAATCTAA ACTGCTGGAA 
AATTCATGTG CCCGAAGTAT TCAGATCCCT GCTGATAGAT CAAAAACAGC TGTGTCAAAA 
TGTCCAGTAT TTCCAATGGC CAGGAGCGTC AGTACTTATG GCCCCTTGGA TAAAGAAGAC 
ACTGGAGGGC AGAAGCTTAT TCCTACAGGC AGCCTGCCAG CAACTTTACA AGGAGCTACA 
GATGCTTTAG GATTGGAATG GCACCTCCCA AGTCCAGATC CCGTCACTGT TCCGTATCTC 
AGTCCTTTGG TGGTGTGGAA GGAACTTGAA AGCTTATTGG AGAATGAAGG CGATCATGCA 
ATAACAGTGG CAGATTTTGT GGACCATCAT CCAATTGTTT TTTGGAACCT AGTGTGGTAT 
TTTAGACGTC TTGACTTGCC CAGTAACTTA CCTGGATTGA TTCTTTCTTC TGAGCATTGT 
AACAAATATT CAAAGATTCC CCGCCACTGT ATGTCTGAAG ATAGTAAATA TGTTTTAATA 
CAAATGTTAT GGGACAATAT GAAATTACAT CAGGATCCGG GTCAGCCCTT GTACATCCTC 
TGGAATGCCC ACAGTCAAAA TCGTACTCTT TTGTTTGAGA CCCAAAAGTA TCCAATGGTC 
CATTTATTGC AAAAGGGTGA TAATTCATTT AACCAAGAAC TATTGAAAAA TATGGTAAAA 
AGCATTAAAA TGAATGATGT CTATGGACCA ATGAGTCAGA TTTTAGAGAC ACTGAATAAA 
TGTCCTCATT TTAAAAGACA GAGGAGTTTA TATAGAGAAA TACTCTTTCT CTCACTTGTG 
GCACTGGGAA GAGAAAACAT TGACATAGAT GCATTTGATA AAGAATACAA GATGGCATAC 
GATCGTCTCA CCCCTAGTCA AGTCAAGAGT ACACACAACT GTGATAGACC ACCAAGTACG 
GGGGTGATGG AATGTCGGAA AACCTTTGGA GAACCTTATC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023510886.1
CDS	586..6210
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVVIKSLGEEVPRDYMCIDLTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGPAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_023655118.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023655118.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGGTGGCC AGTCTGATCT TGGATATAAC TCATTATCTA AGGATGAAGT TAGAAGAGGA 
CATACATCTA CTGAAGACAT TCAAGAAGAA AAAGATAGGA AAGGGAGTGA TTCTAGTTCT 
TTGTCAGAGA GTGAGAGTGC AAAAGGAAGT GCTGATTGTC TGCCTAAGCT CAGTTTTCAA 
AGTTCTTCCA GTATAGTTCG CCTCAGTGGT GCAAATAACA ACAGTGCTGA TAACACCTCA 
GGGAAAAGCA CAGAAAGCAC ATCTGAGCTG CTTGTAATAT CATCTTTTGA GGACACAAAT 
GAAACAGGAA ACATTCAAAG TAGATGCTTC AGGAAAAGAC ATAAAAGTGA CAATGAACCT 
AATTTGCAGC AGCAAATGTC CTGGGGAAGT AGAAACCGCA ATCTTAGTGG AGGAGTACTG 
ATGGGACTTA TGCTCAATAG GATGAACCAG GAAGCAAACC CTGGTGATAT GGTTGAAAAA 
TTAGGAGCTG ATGCAAAAAT TCTTTCAAAT GTTATCTCAA AAAGCACAAG ACCAAATAGT 
CTTGACATTG GAAAGCCACC TGTAAGATCA AAAAGAGACA GTCTAGAGAA GGAATCTAGT 
GATGATGATA CACCTTTTGA TGGTTCTAAC TGTTTGGCAG ATAAAGTGGA TTCTCCCGTT 
ATTTTTGACT TAGAAGACTT AGACAATGAA ACAGATGGAT CAAAAGTGGG ATATGTTGCT 
ACACAAAATC CCAAGAGGAT TCAGCGTATG AGCAGCAGCT TTTCAGTAAA ACCTTTTGAA 
AAAACTGATG TTGCAACAGG ATTTGATCCC CTCTCTCTTT TGGTTGCTGA GACTGAACAG 
CAGCAAAAAG AGGAGGAGGA AGAGGGGGAA GATGATAGCA AAAGCATTTC AACACCATCT 
GCCAGACGTG ATTTAGCTGA AGAAATTGAG ATGTATATGA ATAACATGAG CAGTCCTTTG 
ACAAGTCGTA CACCAAGCAT TGATTTACAA CGGGCATGTG ATGATAAATT AACCAATAAG 
AAAAGTCCAA CATTGGTCAA GGCATGCAGA AGATCTAGCC TGCCTCCTAA TTCTCCTAGG 
CCAGTGAGAC TTACCAAATC TAAAAGTTAC ACAAAAAGTG AGGACAGGCC AAGGGATAGA 
CTCTGGTCTT CTCCAGCCTT CTCACCCACT TGCCCTTTTA GGGAAGAATC TCAAGATGCA 
TTACCCCACT CATCACCTTC ATTTAATTTG GATACACTAC TAGTACCTAA ACTGGATGTT 
TTAAGGAACA GCATGTTCAC TGCTGGAAAA GGAGTAGCCG AGAAAGCAAG CAAATGGTAT 
TCAAGGTTCA CCATGTATAC TACATCATCT AAGGATCAAA GTTCTGATCG TGCCAGTCTT 
TCTTCAGTGG GAGCCCAGGA TTCTGAATCT GCCTCTTTAA CAGATGAAGA TGTCTGCCAC 
GAGCTGGAAG GAGCCGCTTC CTCCCAAGAG AGCAGTGCCA CTTCAGGGAC CAAGGGAATT 
GATTTCAGCA GAACAAGCCT GGGAAGTTCT GCCTCCTTAG AAGGATCCCT GTCAAAGTTC 
GCCTTACCTG GGAAATCAGA AGTGGCATCT TCCTACAACA CGAGTAATAC AAATATCTTC 
CAGAACTATG CAATGGAGGT TCTCATCTCA AGTTGCTCTC GGTGTAGAAC TTGTGACTGC 
CTTGTCCATG ATGAGGAAAT CATGGCTGGC TGGACAGCAG ATGACTCAAA TCTCAATACC 
ACATGCCCGT TTTGTGGCAA TCTTTTCTTG CCCTTTTTGA ATATTGAAAT AAGAGATTTA 
AGACGACCCG GAAAATACTT TTTGAAGTCA AGCCCATCTG CAGAAAATAT GCATTTTCCG 
TCTTCCTTTT CAAGTCAGAC AAGACAGTCT TGTATCTCAA CATCAGCCTC TGGTCTTGAC 
AAGTCTGTTA TCTCCGTTCC AGGGAATTTT GATCTACATA GCAAATCTAA ACTGCTGGAA 
AATTCATGTG CCCGAAGTAT TCAGATCCCT GCTGATAGAT CAAAAACAGC TGTGTCAAAA 
TGTCCAGTAT TTCCAATGGC CAGGAGCGTC AGTACTTATG GCCCCTTGGA TAAAGAAGAC 
ACTGGAGGGC AGAAGCTTAT TCCTACAGGC AGCCTGCCAG CAACTTTACA AGGAGCTACA 
GATGCTTTAG GATTGGAATG GCACCTCCCA AGTCCAGATC CCGTCACTGT TCCGTATCTC 
AGTCCTTTGG TGGTGTGGAA GGAACTTGAA AGCTTATTGG AGAATGAAGG CGATCATGCA 
ATAACAGTGG CAGATTTTGT GGACCATCAT CCAATTGTTT TTTGGAACCT AGTGTGGTAT 
TTTAGACGTC TTGACTTGCC CAGTAACTTA CCTGGATTGA TTCTTTCTTC TGAGCATTGT 
AACAAATATT CAAAGATTCC CCGCCACTGT ATGTCTGAAG ATAGTAAATA TGTTTTAATA 
CAAATGTTAT GGGACAATAT GAAATTACAT CAGGATCCGG GTCAGCCCTT GTACATCCTC 
TGGAATGCCC ACAGTCAAAA TCGTACTCTT TTGTTTGAGA CCCAAAAGTA TCCAATGGTC 
CATTTATTGC AAAAGGGTGA TAATTCATTT AACCAAGAAC TATTGAAAAA TATGGTAAAA 
AGCATTAAAA TGAATGATGT CTATGGACCA ATGAGTCAGA TTTTAGAGAC ACTGAATAAA 
TGTCCTCATT TTAAAAGACA GAGGAGTTTA TATAGAGAAA TACTCTTTCT CTCACTTGTG 
GCACTGGGAA GAGAAAACAT TGACATAGAT GCATTTGATA AAGAATACAA GATGGCATAC 
GATCGTCTCA CCCCTAGTCA AGTCAAGAGT ACACACAACT GTGATAGACC ACCAAGTACG 
GGGGTGATGG AATGTCGGAA AACCTTTGGA GAACCTTATC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_023655138.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023510906.1 Equus caballus DENN domain containing 4A (DENND4A), transcript variant X10, mRNA.

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CloneID	OHb41923
Clone ID Related Accession (Same CDS sequence)	XM_023655138.1
Accession Version	XM_023655138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGTCT GGGTAAAACT GCTTGCTTTT TGCGGAGCAC CTATCATATG CAGTCAGCTG 
AGAGCGACTA TTCAACATTT CACGGAGTCT TCTCAGGAGG ACAGGGTTTT ATATGACTGG 
AAGGAAAGAT TGAAACAAGG GTGTGAAATT ATTCAGAGTA CTCCCTATGG ACGCCCTGCA 
AATATTAGTG GCAGTACCTC ATCACAAAGA ATTTATATCA CTTACCGAAG AGCCTCTGAA 
AACATGACTC AGAATACTTT GGCTGTCACA GACATATGTA TTATTATACC CAGTAAAGGA 
GAAAGCCCAC CACACACATT CTGCAAAGTC GACAAGAATC TCAATAACAG TATGTGGGGA 
CCAGCAGTGT ACTTATGTTA TAAAAAATCG GTGGCAAAGA CTAACACTAT ATCTTATAAA 
GCTGGTCTAA TTTGTAGATA TCCTCAAGAA GATTATGAGT CATTCTCACT CCCAGAATCT 
GTTCCCCTAT TTTGTTTACC AATGGGTGCA ACTATTGAGT CTTGGCCACC AAATAGCAAA 
TACCCTCTCC CTGTATTTTC TACATTTGTG TTAACTGGAG CCTCAGCTGA AAAGGTCTAT 
GGTGCTGCTA TTCAGTTTTA TGAACCATAC TCTGAGGAGA ATCTCACAGA AAAACAGAGA 
CTTCTTTTGG GTTTAGCTTC ATCGGCAGAT GGGAAGTCTG ATAGTTCCAA AACAATTCAC 
ACTAATAAAT GCATCTGTCT TCTTTCTCAC TGGCCTTTTT TTGATGCATT CAGGAAGTTT 
CTGACCTTTT TGTATCGTTA CTCCATCTCT GGACCTCATG TCCTTCCAAT TGAGAAGCAT 
ATTTCTCATT TTATGCATAA AGTTCCTTTT CCATCTCCTC AGAGGCCACG GATTTTAGTT 
CAGTTATCAC CGCATGATAA TCTGATTCTC AGTCAGCCTG TTTCTTCACC TCTTCCACTA 
AGTGGTGGCA AGTTTTCAAC ACTGCTTCAG AATTTAGGCC CTGAAAATGC TGTGACACTA 
CTGGTGTTTG CAGTAACAGA ACATAAGATT CTCATCCATT CCTTACGGCC TTCCGTGCTT 
ACTAGTGTGA CAGAAGCATT AGTGTCTATG ATTTTCCCTT TTCACTGGCC ATGCCCGTAT 
GTTCCTCTGT GCCCGCTGGC TTTAGCAGAT GTCTTGAGTG CACCATGTCC ATTCATAGTA 
GGAATTGATT CAAGATACTT TGATCTCTAT GACCCTCCAC CAGATGTTAG TTGTGTTGAC 
CTGGATACCA ATACCATTTC TCAACCTGGT GACAAGAAAA ATGTAGCCTG GAAGATACTT 
CCAAAGAAAC CATGTAAAAA TCTGATGAAC ACACTGAATA ATTTGCACCA GCAATTGGCA 
AAATTACAGC AGAGACCAAG AGAAGATGGA CTAATGGACT TAGCGATGAA TGACTATGAT 
TTTAATTCTG GAAAGAGGTT GCACATGATA GATTTGGAAA TCCAAGAGGC ATTTTTGTGT 
TTCATGGCCT CTATTTTAAA AGGTTACAGA TCATACTTAA GACCAATAAC ACAGGCCCCA 
TCTGAGACAG CCACAGATGC AACCTCTCTT TTTGCCCTGC AAGCTTTCTT AAGAAGCCGG 
GACCGATCAC ATCAAAAATT CTATAACATG ATGACCAAAA CACAAATGTT TATTCGCTTC 
ATTGAGGAAT GTTCTTTTGT CAGTGATAAA GATGCAAGCC TGGCATTTTT TGATGATTGT 
GTAGATAAAG TGGACATGGA CAAGAATGGT GAAATGCGAC TTATAGAACT GGATGAATCT 
TTCAAAAGTG AACATACTGT CTTTGTAACA CCTCCTGAGA TTCCCCATCT ACCCAATGGT 
GAAGAACCCC CTTTACAGTA CAGCTATAAT GGATTTCCAG TTCTTAGGAG CAGCTTATTT 
GAAAGACCTG AAGGGTTTCT GCAGACACGA AAGAGCAAAT TGCCTTCAAA ACCAAGTAGT 
CCTGGTAGCC CTTCTCCCGT GTTCCGAAGA ACAAAACAGG AAATTAAATC AGCCCATAAA 
ATTGCCAAGA GGTATTCTTC CATTCCTCAG ATGTGGTCTA GGTGTCTACT GCGTCACTGT 
TATGGACTGT GGTTTATTTG TCTCCCAGCT TATGTGAAAG TATGTCATTC AAAGGTCAGG 
GCTCTGAAAA CGGGATATGA TGTGCTTAAG AAAATGCAGT CAAAGAAGAT GGATCCACCC 
GATGAGGTGT GCTACCGCAT TCTTATGCAG CTCTGTGGAC AGTATGATCA GCCGGTGCTT 
GCAGTTCGAG TGCTTTTTGA AATGCAGAAA GCTGGTATTG ACCCCAATGC CATTACTTAT 
GGTTATTATA ATAAGGCTGT TTTGGAAAGT ACATGGCCTT CAAGAAGTCG TAGTGGCTAT 
TTTCTTTGGA CAAAAGTAAG AAATGTTGTT TTAGGAGTGG CACAGTTCAA AAGAGCTTTA 
AAGAAACATG CGCATTTATC ACAAACAACT CTCTCAGCAG ATGGCAGTGA CCTGGATGCT 
GTTAGTCATG GCAGCATGGA TAGCGGTCAT GGGACACACA CTGTGGAGCA GGCACCTTTT 
AATACGGGTT TAATCAAAGT ATATGCTACT GATGATAGAT CTAGTACAGG TGGCCAGTCT 
GATCTTGGAT ATAACTCATT ATCTAAGGAT GAAGTTAGAA GAGGACATAC ATCTACTGAA 
GACATTCAAG AAGAAAAAGA TAGGAAAGGG AGTGATTCTA GTTCTTTGTC AGAGAGTGAG 
AGTGCAAAAG GAAGTGCTGA TTGTCTGCCT AAGCTCAGTT TTCAAAGTTC TTCCAGTATA 
GTTCGCCTCA GTGGTGCAAA TAACAACAGT GCTGATAACA CCTCAGGGAA AAGCACAGAA 
AGCACATCTG AGCTGCTTGT AATATCATCT TTTGAGGACA CAAATGAAAC AGGAAACATT 
CAAAGTAGAT GCTTCAGGAA AAGACATAAA AGTGACAATG AACCTAATTT GCAGCAGCAA 
ATGTCCTGGG GAAGTAGAAA CCGCAATCTT AGTGGAGGAG TACTGATGGG ACTTATGCTC 
AATAGGATGA ACCAGGAAGC AAACCCTGGT GATATGGTTG AAAAATTAGG AGCTGATGCA 
AAAATTCTTT CAAATGTTAT CTCAAAAAGC ACAAGACCAA ATAGTCTTGA CATTGGAAAG 
CCACCTGTAA GATCAAAAAG AGACAGTCTA GAGAAGGAAT CTAGTGATGA TGATACACCT 
TTTGATGGTT CTAACTGTTT GGCAGATAAA GTGGATTCTC CCGTTATTTT TGACTTAGAA 
GACTTAGACA ATGAAACAGA TGGATCAAAA GTGGGATATG TTGCTACACA AAATCCCAAG 
AGGATTCAGC GTATGAGCAG CAGCTTTTCA GTAAAACCTT TTGAAAAAAC TGATGTTGCA 
ACAGGATTTG ATCCCCTCTC TCTTTTGGTT GCTGAGACTG AACAGCAGCA AAAAGAGGAG 
GAGGAAGAGG GGGAAGATGA TAGCAAAAGC ATTTCAACAC CATCTGCCAG ACGTGATTTA 
GCTGAAGAAA TTGAGATGTA TATGAATAAC ATGAGCAGTC CTTTGACAAG TCGTACACCA 
AGCATTGATT TACAACGGGC ATGTGATGAT AAATTAACCA ATAAGAAAAG TCCAACATTG 
GTCAAGGCAT GCAGAAGATC TAGCCTGCCT CCTAATTCTC CTAGGCCAGT GAGACTTACC 
AAATCTAAAA GTTACACAAA AAGTGAGGAC AGGCCAAGGG ATAGACTCTG GTCTTCTCCA 
GCCTTCTCAC CCACTTGCCC TTTTAGGGAA GAATCTCAAG ATGCATTACC CCACTCATCA 
CCTTCATTTA ATTTGGATAC ACTACTAGTA CCTAAACTGG ATGTTTTAAG GAACAGCATG 
TTCACTGCTG GAAAAGGAGT AGCCGAGAAA GCAAGCAAAT GGTATTCAAG GTTCACCATG 
TATACTACAT CATCTAAGGA TCAAAGTTCT GATCGTGCCA GTCTTTCTTC AGTGGGAGCC 
CAGGATTCTG AATCTGCCTC TTTAACAGAT GAAGATGTCT GCCACGAGCT GGAAGGAGCC 
GCTTCCTCCC AAGAGAGCAG TGCCACTTCA GGGACCAAGG GAATTGATTT CAGCAGAACA 
AGCCTGGGAA GTTCTGCCTC CTTAGAAGGA TCCCTGTCAA AGTTCGCCTT ACCTGGGAAA 
TCAGAAGTGG CATCTTCCTA CAACACGAGT AATACAAATA TCTTCCAGAA CTATGCAATG 
GAGGTTCTCA TCTCAAGTTG CTCTCGGTGT AGAACTTGTG ACTGCCTTGT CCATGATGAG 
GAAATCATGG CTGGCTGGAC AGCAGATGAC TCAAATCTCA ATACCACATG CCCGTTTTGT 
GGCAATCTTT TCTTGCCCTT TTTGAATATT GAAATAAGAG ATTTAAGACG ACCCGGAAAA 
TACTTTTTGA AGTCAAGCCC ATCTGCAGAA AATATGCATT TTCCGTCTTC CTTTTCAAGT 
CAGACAAGAC AGTCTTGTAT CTCAACATCA GCCTCTGGTC TTGACAAGTC TGTTATCTCC 
GTTCCAGGGA ATTTTGATCT ACATAGCAAA TCTAAACTGC TGGAAAATTC ATGTGCCCGA 
AGTATTCAGA TCCCTGCTGA TAGATCAAAA ACAGCTGTGT CAAAATGTCC AGTATTTCCA 
ATGGCCAGGA GCGTCAGTAC TTATGGCCCC TTGGATAAAG AAGACACTGG AGGGCAGAAG 
CTTATTCCTA CAGGCAGCCT GCCAGCAACT TTACAAGGAG CTACAGATGC TTTAGGATTG 
GAATGGCACC TCCCAAGTCC AGATCCCGTC ACTGTTCCGT ATCTCAGTCC TTTGGTGGTG 
TGGAAGGAAC TTGAAAGCTT ATTGGAGAAT GAAGGCGATC ATGCAATAAC AGTGGCAGAT 
TTTGTGGACC ATCATCCAAT TGTTTTTTGG AACCTAGTGT GGTATTTTAG ACGTCTTGAC 
TTGCCCAGTA ACTTACCTGG ATTGATTCTT TCTTCTGAGC ATTGTAACAA ATATTCAAAG 
ATTCCCCGCC ACTGTATGTC TGAAGATAGT AAATATGTTT TAATACAAAT GTTATGGGAC 
AATATGAAAT TACATCAGGA TCCGGGTCAG CCCTTGTACA TCCTCTGGAA TGCCCACAGT 
CAAAATCGTA CTCTTTTGTT TGAGACCCAA AAGTATCCAA TGGTCCATTT ATTGCAAAAG 
GGTGATAATT CATTTAACCA AGAACTATTG AAAAATATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAGATTTTA GAGACACTGA ATAAATGTCC TCATTTTAAA 
AGACAGAGGA GTTTATATAG AGAAATACTC TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGACA TAGATGCATT TGATAAAGAA TACAAGATGG CATACGATCG TCTCACCCCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACGGGGGT GATGGAATGT 
CGGAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023510906.1
CDS	310..5859
Translation	MFVWVKLLAFCGAPIICSQLRATIQHFTESSQEDRVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGPAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_023655138.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023655138.1

ORF Insert Sequence:

ATGTTCGTCT GGGTAAAACT GCTTGCTTTT TGCGGAGCAC CTATCATATG CAGTCAGCTG 
AGAGCGACTA TTCAACATTT CACGGAGTCT TCTCAGGAGG ACAGGGTTTT ATATGACTGG 
AAGGAAAGAT TGAAACAAGG GTGTGAAATT ATTCAGAGTA CTCCCTATGG ACGCCCTGCA 
AATATTAGTG GCAGTACCTC ATCACAAAGA ATTTATATCA CTTACCGAAG AGCCTCTGAA 
AACATGACTC AGAATACTTT GGCTGTCACA GACATATGTA TTATTATACC CAGTAAAGGA 
GAAAGCCCAC CACACACATT CTGCAAAGTC GACAAGAATC TCAATAACAG TATGTGGGGA 
CCAGCAGTGT ACTTATGTTA TAAAAAATCG GTGGCAAAGA CTAACACTAT ATCTTATAAA 
GCTGGTCTAA TTTGTAGATA TCCTCAAGAA GATTATGAGT CATTCTCACT CCCAGAATCT 
GTTCCCCTAT TTTGTTTACC AATGGGTGCA ACTATTGAGT CTTGGCCACC AAATAGCAAA 
TACCCTCTCC CTGTATTTTC TACATTTGTG TTAACTGGAG CCTCAGCTGA AAAGGTCTAT 
GGTGCTGCTA TTCAGTTTTA TGAACCATAC TCTGAGGAGA ATCTCACAGA AAAACAGAGA 
CTTCTTTTGG GTTTAGCTTC ATCGGCAGAT GGGAAGTCTG ATAGTTCCAA AACAATTCAC 
ACTAATAAAT GCATCTGTCT TCTTTCTCAC TGGCCTTTTT TTGATGCATT CAGGAAGTTT 
CTGACCTTTT TGTATCGTTA CTCCATCTCT GGACCTCATG TCCTTCCAAT TGAGAAGCAT 
ATTTCTCATT TTATGCATAA AGTTCCTTTT CCATCTCCTC AGAGGCCACG GATTTTAGTT 
CAGTTATCAC CGCATGATAA TCTGATTCTC AGTCAGCCTG TTTCTTCACC TCTTCCACTA 
AGTGGTGGCA AGTTTTCAAC ACTGCTTCAG AATTTAGGCC CTGAAAATGC TGTGACACTA 
CTGGTGTTTG CAGTAACAGA ACATAAGATT CTCATCCATT CCTTACGGCC TTCCGTGCTT 
ACTAGTGTGA CAGAAGCATT AGTGTCTATG ATTTTCCCTT TTCACTGGCC ATGCCCGTAT 
GTTCCTCTGT GCCCGCTGGC TTTAGCAGAT GTCTTGAGTG CACCATGTCC ATTCATAGTA 
GGAATTGATT CAAGATACTT TGATCTCTAT GACCCTCCAC CAGATGTTAG TTGTGTTGAC 
CTGGATACCA ATACCATTTC TCAACCTGGT GACAAGAAAA ATGTAGCCTG GAAGATACTT 
CCAAAGAAAC CATGTAAAAA TCTGATGAAC ACACTGAATA ATTTGCACCA GCAATTGGCA 
AAATTACAGC AGAGACCAAG AGAAGATGGA CTAATGGACT TAGCGATGAA TGACTATGAT 
TTTAATTCTG GAAAGAGGTT GCACATGATA GATTTGGAAA TCCAAGAGGC ATTTTTGTGT 
TTCATGGCCT CTATTTTAAA AGGTTACAGA TCATACTTAA GACCAATAAC ACAGGCCCCA 
TCTGAGACAG CCACAGATGC AACCTCTCTT TTTGCCCTGC AAGCTTTCTT AAGAAGCCGG 
GACCGATCAC ATCAAAAATT CTATAACATG ATGACCAAAA CACAAATGTT TATTCGCTTC 
ATTGAGGAAT GTTCTTTTGT CAGTGATAAA GATGCAAGCC TGGCATTTTT TGATGATTGT 
GTAGATAAAG TGGACATGGA CAAGAATGGT GAAATGCGAC TTATAGAACT GGATGAATCT 
TTCAAAAGTG AACATACTGT CTTTGTAACA CCTCCTGAGA TTCCCCATCT ACCCAATGGT 
GAAGAACCCC CTTTACAGTA CAGCTATAAT GGATTTCCAG TTCTTAGGAG CAGCTTATTT 
GAAAGACCTG AAGGGTTTCT GCAGACACGA AAGAGCAAAT TGCCTTCAAA ACCAAGTAGT 
CCTGGTAGCC CTTCTCCCGT GTTCCGAAGA ACAAAACAGG AAATTAAATC AGCCCATAAA 
ATTGCCAAGA GGTATTCTTC CATTCCTCAG ATGTGGTCTA GGTGTCTACT GCGTCACTGT 
TATGGACTGT GGTTTATTTG TCTCCCAGCT TATGTGAAAG TATGTCATTC AAAGGTCAGG 
GCTCTGAAAA CGGGATATGA TGTGCTTAAG AAAATGCAGT CAAAGAAGAT GGATCCACCC 
GATGAGGTGT GCTACCGCAT TCTTATGCAG CTCTGTGGAC AGTATGATCA GCCGGTGCTT 
GCAGTTCGAG TGCTTTTTGA AATGCAGAAA GCTGGTATTG ACCCCAATGC CATTACTTAT 
GGTTATTATA ATAAGGCTGT TTTGGAAAGT ACATGGCCTT CAAGAAGTCG TAGTGGCTAT 
TTTCTTTGGA CAAAAGTAAG AAATGTTGTT TTAGGAGTGG CACAGTTCAA AAGAGCTTTA 
AAGAAACATG CGCATTTATC ACAAACAACT CTCTCAGCAG ATGGCAGTGA CCTGGATGCT 
GTTAGTCATG GCAGCATGGA TAGCGGTCAT GGGACACACA CTGTGGAGCA GGCACCTTTT 
AATACGGGTT TAATCAAAGT ATATGCTACT GATGATAGAT CTAGTACAGG TGGCCAGTCT 
GATCTTGGAT ATAACTCATT ATCTAAGGAT GAAGTTAGAA GAGGACATAC ATCTACTGAA 
GACATTCAAG AAGAAAAAGA TAGGAAAGGG AGTGATTCTA GTTCTTTGTC AGAGAGTGAG 
AGTGCAAAAG GAAGTGCTGA TTGTCTGCCT AAGCTCAGTT TTCAAAGTTC TTCCAGTATA 
GTTCGCCTCA GTGGTGCAAA TAACAACAGT GCTGATAACA CCTCAGGGAA AAGCACAGAA 
AGCACATCTG AGCTGCTTGT AATATCATCT TTTGAGGACA CAAATGAAAC AGGAAACATT 
CAAAGTAGAT GCTTCAGGAA AAGACATAAA AGTGACAATG AACCTAATTT GCAGCAGCAA 
ATGTCCTGGG GAAGTAGAAA CCGCAATCTT AGTGGAGGAG TACTGATGGG ACTTATGCTC 
AATAGGATGA ACCAGGAAGC AAACCCTGGT GATATGGTTG AAAAATTAGG AGCTGATGCA 
AAAATTCTTT CAAATGTTAT CTCAAAAAGC ACAAGACCAA ATAGTCTTGA CATTGGAAAG 
CCACCTGTAA GATCAAAAAG AGACAGTCTA GAGAAGGAAT CTAGTGATGA TGATACACCT 
TTTGATGGTT CTAACTGTTT GGCAGATAAA GTGGATTCTC CCGTTATTTT TGACTTAGAA 
GACTTAGACA ATGAAACAGA TGGATCAAAA GTGGGATATG TTGCTACACA AAATCCCAAG 
AGGATTCAGC GTATGAGCAG CAGCTTTTCA GTAAAACCTT TTGAAAAAAC TGATGTTGCA 
ACAGGATTTG ATCCCCTCTC TCTTTTGGTT GCTGAGACTG AACAGCAGCA AAAAGAGGAG 
GAGGAAGAGG GGGAAGATGA TAGCAAAAGC ATTTCAACAC CATCTGCCAG ACGTGATTTA 
GCTGAAGAAA TTGAGATGTA TATGAATAAC ATGAGCAGTC CTTTGACAAG TCGTACACCA 
AGCATTGATT TACAACGGGC ATGTGATGAT AAATTAACCA ATAAGAAAAG TCCAACATTG 
GTCAAGGCAT GCAGAAGATC TAGCCTGCCT CCTAATTCTC CTAGGCCAGT GAGACTTACC 
AAATCTAAAA GTTACACAAA AAGTGAGGAC AGGCCAAGGG ATAGACTCTG GTCTTCTCCA 
GCCTTCTCAC CCACTTGCCC TTTTAGGGAA GAATCTCAAG ATGCATTACC CCACTCATCA 
CCTTCATTTA ATTTGGATAC ACTACTAGTA CCTAAACTGG ATGTTTTAAG GAACAGCATG 
TTCACTGCTG GAAAAGGAGT AGCCGAGAAA GCAAGCAAAT GGTATTCAAG GTTCACCATG 
TATACTACAT CATCTAAGGA TCAAAGTTCT GATCGTGCCA GTCTTTCTTC AGTGGGAGCC 
CAGGATTCTG AATCTGCCTC TTTAACAGAT GAAGATGTCT GCCACGAGCT GGAAGGAGCC 
GCTTCCTCCC AAGAGAGCAG TGCCACTTCA GGGACCAAGG GAATTGATTT CAGCAGAACA 
AGCCTGGGAA GTTCTGCCTC CTTAGAAGGA TCCCTGTCAA AGTTCGCCTT ACCTGGGAAA 
TCAGAAGTGG CATCTTCCTA CAACACGAGT AATACAAATA TCTTCCAGAA CTATGCAATG 
GAGGTTCTCA TCTCAAGTTG CTCTCGGTGT AGAACTTGTG ACTGCCTTGT CCATGATGAG 
GAAATCATGG CTGGCTGGAC AGCAGATGAC TCAAATCTCA ATACCACATG CCCGTTTTGT 
GGCAATCTTT TCTTGCCCTT TTTGAATATT GAAATAAGAG ATTTAAGACG ACCCGGAAAA 
TACTTTTTGA AGTCAAGCCC ATCTGCAGAA AATATGCATT TTCCGTCTTC CTTTTCAAGT 
CAGACAAGAC AGTCTTGTAT CTCAACATCA GCCTCTGGTC TTGACAAGTC TGTTATCTCC 
GTTCCAGGGA ATTTTGATCT ACATAGCAAA TCTAAACTGC TGGAAAATTC ATGTGCCCGA 
AGTATTCAGA TCCCTGCTGA TAGATCAAAA ACAGCTGTGT CAAAATGTCC AGTATTTCCA 
ATGGCCAGGA GCGTCAGTAC TTATGGCCCC TTGGATAAAG AAGACACTGG AGGGCAGAAG 
CTTATTCCTA CAGGCAGCCT GCCAGCAACT TTACAAGGAG CTACAGATGC TTTAGGATTG 
GAATGGCACC TCCCAAGTCC AGATCCCGTC ACTGTTCCGT ATCTCAGTCC TTTGGTGGTG 
TGGAAGGAAC TTGAAAGCTT ATTGGAGAAT GAAGGCGATC ATGCAATAAC AGTGGCAGAT 
TTTGTGGACC ATCATCCAAT TGTTTTTTGG AACCTAGTGT GGTATTTTAG ACGTCTTGAC 
TTGCCCAGTA ACTTACCTGG ATTGATTCTT TCTTCTGAGC ATTGTAACAA ATATTCAAAG 
ATTCCCCGCC ACTGTATGTC TGAAGATAGT AAATATGTTT TAATACAAAT GTTATGGGAC 
AATATGAAAT TACATCAGGA TCCGGGTCAG CCCTTGTACA TCCTCTGGAA TGCCCACAGT 
CAAAATCGTA CTCTTTTGTT TGAGACCCAA AAGTATCCAA TGGTCCATTT ATTGCAAAAG 
GGTGATAATT CATTTAACCA AGAACTATTG AAAAATATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAGATTTTA GAGACACTGA ATAAATGTCC TCATTTTAAA 
AGACAGAGGA GTTTATATAG AGAAATACTC TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGACA TAGATGCATT TGATAAAGAA TACAAGATGG CATACGATCG TCTCACCCCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACGGGGGT GATGGAATGT 
CGGAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_023655147.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023510915.1 Equus caballus DENN domain containing 4A (DENND4A), transcript variant X11, mRNA.

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CloneID	OHb41924
Clone ID Related Accession (Same CDS sequence)	XM_023655147.1 , XM_023655154.1
Accession Version	XM_023655147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCAA CTATTGAGTC TTGGCCACCA AATAGCAAAT ACCCTCTCCC TGTATTTTCT 
ACATTTGTGT TAACTGGAGC CTCAGCTGAA AAGGTCTATG GTGCTGCTAT TCAGTTTTAT 
GAACCATACT CTGAGGAGAA TCTCACAGAA AAACAGAGAC TTCTTTTGGG TTTAGCTTCA 
TCGGCAGATG GGAAGTCTGA TAGTTCCAAA ACAATTCACA CTAATAAATG CATCTGTCTT 
CTTTCTCACT GGCCTTTTTT TGATGCATTC AGGAAGTTTC TGACCTTTTT GTATCGTTAC 
TCCATCTCTG GACCTCATGT CCTTCCAATT GAGAAGCATA TTTCTCATTT TATGCATAAA 
GTTCCTTTTC CATCTCCTCA GAGGCCACGG ATTTTAGTTC AGTTATCACC GCATGATAAT 
CTGATTCTCA GTCAGCCTGT TTCTTCACCT CTTCCACTAA GTGGTGGCAA GTTTTCAACA 
CTGCTTCAGA ATTTAGGCCC TGAAAATGCT GTGACACTAC TGGTGTTTGC AGTAACAGAA 
CATAAGATTC TCATCCATTC CTTACGGCCT TCCGTGCTTA CTAGTGTGAC AGAAGCATTA 
GTGTCTATGA TTTTCCCTTT TCACTGGCCA TGCCCGTATG TTCCTCTGTG CCCGCTGGCT 
TTAGCAGATG TCTTGAGTGC ACCATGTCCA TTCATAGTAG GAATTGATTC AAGATACTTT 
GATCTCTATG ACCCTCCACC AGATGTTAGT TGTGTTGACC TGGATACCAA TACCATTTCT 
CAACCTGGTG ACAAGAAAAA TGTAGCCTGG AAGATACTTC CAAAGAAACC ATGTAAAAAT 
CTGATGAACA CACTGAATAA TTTGCACCAG CAATTGGCAA AATTACAGCA GAGACCAAGA 
GAAGATGGAC TAATGGACTT AGCGATGAAT GACTATGATT TTAATTCTGG AAAGAGGTTG 
CACATGATAG ATTTGGAAAT CCAAGAGGCA TTTTTGTGTT TCATGGCCTC TATTTTAAAA 
GGTTACAGAT CATACTTAAG ACCAATAACA CAGGCCCCAT CTGAGACAGC CACAGATGCA 
ACCTCTCTTT TTGCCCTGCA AGCTTTCTTA AGAAGCCGGG ACCGATCACA TCAAAAATTC 
TATAACATGA TGACCAAAAC ACAAATGTTT ATTCGCTTCA TTGAGGAATG TTCTTTTGTC 
AGTGATAAAG ATGCAAGCCT GGCATTTTTT GATGATTGTG TAGATAAAGT GGACATGGAC 
AAGAATGGTG AAATGCGACT TATAGAACTG GATGAATCTT TCAAAAGTGA ACATACTGTC 
TTTGTAACAC CTCCTGAGAT TCCCCATCTA CCCAATGGTG AAGAACCCCC TTTACAGTAC 
AGCTATAATG GATTTCCAGT TCTTAGGAGC AGCTTATTTG AAAGACCTGA AGGGTTTCTG 
CAGACACGAA AGAGCAAATT GCCTTCAAAA CCAAGTAGTC CTGGTAGCCC TTCTCCCGTG 
TTCCGAAGAA CAAAACAGGA AATTAAATCA GCCCATAAAA TTGCCAAGAG GTATTCTTCC 
ATTCCTCAGA TGTGGTCTAG GTGTCTACTG CGTCACTGTT ATGGACTGTG GTTTATTTGT 
CTCCCAGCTT ATGTGAAAGT ATGTCATTCA AAGGTCAGGG CTCTGAAAAC GGGATATGAT 
GTGCTTAAGA AAATGCAGTC AAAGAAGATG GATCCACCCG ATGAGGTGTG CTACCGCATT 
CTTATGCAGC TCTGTGGACA GTATGATCAG CCGGTGCTTG CAGTTCGAGT GCTTTTTGAA 
ATGCAGAAAG CTGGTATTGA CCCCAATGCC ATTACTTATG GTTATTATAA TAAGGCTGTT 
TTGGAAAGTA CATGGCCTTC AAGAAGTCGT AGTGGCTATT TTCTTTGGAC AAAAGTAAGA 
AATGTTGTTT TAGGAGTGGC ACAGTTCAAA AGAGCTTTAA AGAAACATGC GCATTTATCA 
CAAACAACTC TCTCAGCAGA TGGCAGTGAC CTGGATGCTG TTAGTCATGG CAGCATGGAT 
AGCGGTCATG GGACACACAC TGTGGAGCAG GCACCTTTTA ATACGGGTTT AATCAAAGTA 
TATGCTACTG ATGATAGATC TAGTACAGGT GGCCAGTCTG ATCTTGGATA TAACTCATTA 
TCTAAGGATG AAGTTAGAAG AGGACATACA TCTACTGAAG ACATTCAAGA AGAAAAAGAT 
AGGAAAGGGA GTGATTCTAG TTCTTTGTCA GAGAGTGAGA GTGCAAAAGG AAGTGCTGAT 
TGTCTGCCTA AGCTCAGTTT TCAAAGTTCT TCCAGTATAG TTCGCCTCAG TGGTGCAAAT 
AACAACAGTG CTGATAACAC CTCAGGGAAA AGCACAGAAA GCACATCTGA GCTGCTTGTA 
ATATCATCTT TTGAGGACAC AAATGAAACA GGAAACATTC AAAGTAGATG CTTCAGGAAA 
AGACATAAAA GTGACAATGA ACCTAATTTG CAGCAGCAAA TGTCCTGGGG AAGTAGAAAC 
CGCAATCTTA GTGGAGGAGT ACTGATGGGA CTTATGCTCA ATAGGATGAA CCAGGAAGCA 
AACCCTGGTG ATATGGTTGA AAAATTAGGA GCTGATGCAA AAATTCTTTC AAATGTTATC 
TCAAAAAGCA CAAGACCAAA TAGTCTTGAC ATTGGAAAGC CACCTGTAAG ATCAAAAAGA 
GACAGTCTAG AGAAGGAATC TAGTGATGAT GATACACCTT TTGATGGTTC TAACTGTTTG 
GCAGATAAAG TGGATTCTCC CGTTATTTTT GACTTAGAAG ACTTAGACAA TGAAACAGAT 
GGATCAAAAG TGGGATATGT TGCTACACAA AATCCCAAGA GGATTCAGCG TATGAGCAGC 
AGCTTTTCAG TAAAACCTTT TGAAAAAACT GATGTTGCAA CAGGATTTGA TCCCCTCTCT 
CTTTTGGTTG CTGAGACTGA ACAGCAGCAA AAAGAGGAGG AGGAAGAGGG GGAAGATGAT 
AGCAAAAGCA TTTCAACACC ATCTGCCAGA CGTGATTTAG CTGAAGAAAT TGAGATGTAT 
ATGAATAACA TGAGCAGTCC TTTGACAAGT CGTACACCAA GCATTGATTT ACAACGGGCA 
TGTGATGATA AATTAACCAA TAAGAAAAGT CCAACATTGG TCAAGGCATG CAGAAGATCT 
AGCCTGCCTC CTAATTCTCC TAGGCCAGTG AGACTTACCA AATCTAAAAG TTACACAAAA 
AGTGAGGACA GGCCAAGGGA TAGACTCTGG TCTTCTCCAG CCTTCTCACC CACTTGCCCT 
TTTAGGGAAG AATCTCAAGA TGCATTACCC CACTCATCAC CTTCATTTAA TTTGGATACA 
CTACTAGTAC CTAAACTGGA TGTTTTAAGG AACAGCATGT TCACTGCTGG AAAAGGAGTA 
GCCGAGAAAG CAAGCAAATG GTATTCAAGG TTCACCATGT ATACTACATC ATCTAAGGAT 
CAAAGTTCTG ATCGTGCCAG TCTTTCTTCA GTGGGAGCCC AGGATTCTGA ATCTGCCTCT 
TTAACAGATG AAGATGTCTG CCACGAGCTG GAAGGAGCCG CTTCCTCCCA AGAGAGCAGT 
GCCACTTCAG GGACCAAGGG AATTGATTTC AGCAGAACAA GCCTGGGAAG TTCTGCCTCC 
TTAGAAGGAT CCCTGTCAAA GTTCGCCTTA CCTGGGAAAT CAGAAGTGGC ATCTTCCTAC 
AACACGAGTA ATACAAATAT CTTCCAGAAC TATGCAATGG AGGTTCTCAT CTCAAGTTGC 
TCTCGGTGTA GAACTTGTGA CTGCCTTGTC CATGATGAGG AAATCATGGC TGGCTGGACA 
GCAGATGACT CAAATCTCAA TACCACATGC CCGTTTTGTG GCAATCTTTT CTTGCCCTTT 
TTGAATATTG AAATAAGAGA TTTAAGACGA CCCGGAAAAT ACTTTTTGAA GTCAAGCCCA 
TCTGCAGAAA ATATGCATTT TCCGTCTTCC TTTTCAAGTC AGACAAGACA GTCTTGTATC 
TCAACATCAG CCTCTGGTCT TGACAAGTCT GTTATCTCCG TTCCAGGGAA TTTTGATCTA 
CATAGCAAAT CTAAACTGCT GGAAAATTCA TGTGCCCGAA GTATTCAGAT CCCTGCTGAT 
AGATCAAAAA CAGCTGTGTC AAAATGTCCA GTATTTCCAA TGGCCAGGAG CGTCAGTACT 
TATGGCCCCT TGGATAAAGA AGACACTGGA GGGCAGAAGC TTATTCCTAC AGGCAGCCTG 
CCAGCAACTT TACAAGGAGC TACAGATGCT TTAGGATTGG AATGGCACCT CCCAAGTCCA 
GATCCCGTCA CTGTTCCGTA TCTCAGTCCT TTGGTGGTGT GGAAGGAACT TGAAAGCTTA 
TTGGAGAATG AAGGCGATCA TGCAATAACA GTGGCAGATT TTGTGGACCA TCATCCAATT 
GTTTTTTGGA ACCTAGTGTG GTATTTTAGA CGTCTTGACT TGCCCAGTAA CTTACCTGGA 
TTGATTCTTT CTTCTGAGCA TTGTAACAAA TATTCAAAGA TTCCCCGCCA CTGTATGTCT 
GAAGATAGTA AATATGTTTT AATACAAATG TTATGGGACA ATATGAAATT ACATCAGGAT 
CCGGGTCAGC CCTTGTACAT CCTCTGGAAT GCCCACAGTC AAAATCGTAC TCTTTTGTTT 
GAGACCCAAA AGTATCCAAT GGTCCATTTA TTGCAAAAGG GTGATAATTC ATTTAACCAA 
GAACTATTGA AAAATATGGT AAAAAGCATT AAAATGAATG ATGTCTATGG ACCAATGAGT 
CAGATTTTAG AGACACTGAA TAAATGTCCT CATTTTAAAA GACAGAGGAG TTTATATAGA 
GAAATACTCT TTCTCTCACT TGTGGCACTG GGAAGAGAAA ACATTGACAT AGATGCATTT 
GATAAAGAAT ACAAGATGGC ATACGATCGT CTCACCCCTA GTCAAGTCAA GAGTACACAC 
AACTGTGATA GACCACCAAG TACGGGGGTG ATGGAATGTC GGAAAACCTT TGGAGAACCT 
TATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023510915.1
CDS	1042..6090
Translation	MGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_023655147.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023655147.1

ORF Insert Sequence:

ATGGGTGCAA CTATTGAGTC TTGGCCACCA AATAGCAAAT ACCCTCTCCC TGTATTTTCT 
ACATTTGTGT TAACTGGAGC CTCAGCTGAA AAGGTCTATG GTGCTGCTAT TCAGTTTTAT 
GAACCATACT CTGAGGAGAA TCTCACAGAA AAACAGAGAC TTCTTTTGGG TTTAGCTTCA 
TCGGCAGATG GGAAGTCTGA TAGTTCCAAA ACAATTCACA CTAATAAATG CATCTGTCTT 
CTTTCTCACT GGCCTTTTTT TGATGCATTC AGGAAGTTTC TGACCTTTTT GTATCGTTAC 
TCCATCTCTG GACCTCATGT CCTTCCAATT GAGAAGCATA TTTCTCATTT TATGCATAAA 
GTTCCTTTTC CATCTCCTCA GAGGCCACGG ATTTTAGTTC AGTTATCACC GCATGATAAT 
CTGATTCTCA GTCAGCCTGT TTCTTCACCT CTTCCACTAA GTGGTGGCAA GTTTTCAACA 
CTGCTTCAGA ATTTAGGCCC TGAAAATGCT GTGACACTAC TGGTGTTTGC AGTAACAGAA 
CATAAGATTC TCATCCATTC CTTACGGCCT TCCGTGCTTA CTAGTGTGAC AGAAGCATTA 
GTGTCTATGA TTTTCCCTTT TCACTGGCCA TGCCCGTATG TTCCTCTGTG CCCGCTGGCT 
TTAGCAGATG TCTTGAGTGC ACCATGTCCA TTCATAGTAG GAATTGATTC AAGATACTTT 
GATCTCTATG ACCCTCCACC AGATGTTAGT TGTGTTGACC TGGATACCAA TACCATTTCT 
CAACCTGGTG ACAAGAAAAA TGTAGCCTGG AAGATACTTC CAAAGAAACC ATGTAAAAAT 
CTGATGAACA CACTGAATAA TTTGCACCAG CAATTGGCAA AATTACAGCA GAGACCAAGA 
GAAGATGGAC TAATGGACTT AGCGATGAAT GACTATGATT TTAATTCTGG AAAGAGGTTG 
CACATGATAG ATTTGGAAAT CCAAGAGGCA TTTTTGTGTT TCATGGCCTC TATTTTAAAA 
GGTTACAGAT CATACTTAAG ACCAATAACA CAGGCCCCAT CTGAGACAGC CACAGATGCA 
ACCTCTCTTT TTGCCCTGCA AGCTTTCTTA AGAAGCCGGG ACCGATCACA TCAAAAATTC 
TATAACATGA TGACCAAAAC ACAAATGTTT ATTCGCTTCA TTGAGGAATG TTCTTTTGTC 
AGTGATAAAG ATGCAAGCCT GGCATTTTTT GATGATTGTG TAGATAAAGT GGACATGGAC 
AAGAATGGTG AAATGCGACT TATAGAACTG GATGAATCTT TCAAAAGTGA ACATACTGTC 
TTTGTAACAC CTCCTGAGAT TCCCCATCTA CCCAATGGTG AAGAACCCCC TTTACAGTAC 
AGCTATAATG GATTTCCAGT TCTTAGGAGC AGCTTATTTG AAAGACCTGA AGGGTTTCTG 
CAGACACGAA AGAGCAAATT GCCTTCAAAA CCAAGTAGTC CTGGTAGCCC TTCTCCCGTG 
TTCCGAAGAA CAAAACAGGA AATTAAATCA GCCCATAAAA TTGCCAAGAG GTATTCTTCC 
ATTCCTCAGA TGTGGTCTAG GTGTCTACTG CGTCACTGTT ATGGACTGTG GTTTATTTGT 
CTCCCAGCTT ATGTGAAAGT ATGTCATTCA AAGGTCAGGG CTCTGAAAAC GGGATATGAT 
GTGCTTAAGA AAATGCAGTC AAAGAAGATG GATCCACCCG ATGAGGTGTG CTACCGCATT 
CTTATGCAGC TCTGTGGACA GTATGATCAG CCGGTGCTTG CAGTTCGAGT GCTTTTTGAA 
ATGCAGAAAG CTGGTATTGA CCCCAATGCC ATTACTTATG GTTATTATAA TAAGGCTGTT 
TTGGAAAGTA CATGGCCTTC AAGAAGTCGT AGTGGCTATT TTCTTTGGAC AAAAGTAAGA 
AATGTTGTTT TAGGAGTGGC ACAGTTCAAA AGAGCTTTAA AGAAACATGC GCATTTATCA 
CAAACAACTC TCTCAGCAGA TGGCAGTGAC CTGGATGCTG TTAGTCATGG CAGCATGGAT 
AGCGGTCATG GGACACACAC TGTGGAGCAG GCACCTTTTA ATACGGGTTT AATCAAAGTA 
TATGCTACTG ATGATAGATC TAGTACAGGT GGCCAGTCTG ATCTTGGATA TAACTCATTA 
TCTAAGGATG AAGTTAGAAG AGGACATACA TCTACTGAAG ACATTCAAGA AGAAAAAGAT 
AGGAAAGGGA GTGATTCTAG TTCTTTGTCA GAGAGTGAGA GTGCAAAAGG AAGTGCTGAT 
TGTCTGCCTA AGCTCAGTTT TCAAAGTTCT TCCAGTATAG TTCGCCTCAG TGGTGCAAAT 
AACAACAGTG CTGATAACAC CTCAGGGAAA AGCACAGAAA GCACATCTGA GCTGCTTGTA 
ATATCATCTT TTGAGGACAC AAATGAAACA GGAAACATTC AAAGTAGATG CTTCAGGAAA 
AGACATAAAA GTGACAATGA ACCTAATTTG CAGCAGCAAA TGTCCTGGGG AAGTAGAAAC 
CGCAATCTTA GTGGAGGAGT ACTGATGGGA CTTATGCTCA ATAGGATGAA CCAGGAAGCA 
AACCCTGGTG ATATGGTTGA AAAATTAGGA GCTGATGCAA AAATTCTTTC AAATGTTATC 
TCAAAAAGCA CAAGACCAAA TAGTCTTGAC ATTGGAAAGC CACCTGTAAG ATCAAAAAGA 
GACAGTCTAG AGAAGGAATC TAGTGATGAT GATACACCTT TTGATGGTTC TAACTGTTTG 
GCAGATAAAG TGGATTCTCC CGTTATTTTT GACTTAGAAG ACTTAGACAA TGAAACAGAT 
GGATCAAAAG TGGGATATGT TGCTACACAA AATCCCAAGA GGATTCAGCG TATGAGCAGC 
AGCTTTTCAG TAAAACCTTT TGAAAAAACT GATGTTGCAA CAGGATTTGA TCCCCTCTCT 
CTTTTGGTTG CTGAGACTGA ACAGCAGCAA AAAGAGGAGG AGGAAGAGGG GGAAGATGAT 
AGCAAAAGCA TTTCAACACC ATCTGCCAGA CGTGATTTAG CTGAAGAAAT TGAGATGTAT 
ATGAATAACA TGAGCAGTCC TTTGACAAGT CGTACACCAA GCATTGATTT ACAACGGGCA 
TGTGATGATA AATTAACCAA TAAGAAAAGT CCAACATTGG TCAAGGCATG CAGAAGATCT 
AGCCTGCCTC CTAATTCTCC TAGGCCAGTG AGACTTACCA AATCTAAAAG TTACACAAAA 
AGTGAGGACA GGCCAAGGGA TAGACTCTGG TCTTCTCCAG CCTTCTCACC CACTTGCCCT 
TTTAGGGAAG AATCTCAAGA TGCATTACCC CACTCATCAC CTTCATTTAA TTTGGATACA 
CTACTAGTAC CTAAACTGGA TGTTTTAAGG AACAGCATGT TCACTGCTGG AAAAGGAGTA 
GCCGAGAAAG CAAGCAAATG GTATTCAAGG TTCACCATGT ATACTACATC ATCTAAGGAT 
CAAAGTTCTG ATCGTGCCAG TCTTTCTTCA GTGGGAGCCC AGGATTCTGA ATCTGCCTCT 
TTAACAGATG AAGATGTCTG CCACGAGCTG GAAGGAGCCG CTTCCTCCCA AGAGAGCAGT 
GCCACTTCAG GGACCAAGGG AATTGATTTC AGCAGAACAA GCCTGGGAAG TTCTGCCTCC 
TTAGAAGGAT CCCTGTCAAA GTTCGCCTTA CCTGGGAAAT CAGAAGTGGC ATCTTCCTAC 
AACACGAGTA ATACAAATAT CTTCCAGAAC TATGCAATGG AGGTTCTCAT CTCAAGTTGC 
TCTCGGTGTA GAACTTGTGA CTGCCTTGTC CATGATGAGG AAATCATGGC TGGCTGGACA 
GCAGATGACT CAAATCTCAA TACCACATGC CCGTTTTGTG GCAATCTTTT CTTGCCCTTT 
TTGAATATTG AAATAAGAGA TTTAAGACGA CCCGGAAAAT ACTTTTTGAA GTCAAGCCCA 
TCTGCAGAAA ATATGCATTT TCCGTCTTCC TTTTCAAGTC AGACAAGACA GTCTTGTATC 
TCAACATCAG CCTCTGGTCT TGACAAGTCT GTTATCTCCG TTCCAGGGAA TTTTGATCTA 
CATAGCAAAT CTAAACTGCT GGAAAATTCA TGTGCCCGAA GTATTCAGAT CCCTGCTGAT 
AGATCAAAAA CAGCTGTGTC AAAATGTCCA GTATTTCCAA TGGCCAGGAG CGTCAGTACT 
TATGGCCCCT TGGATAAAGA AGACACTGGA GGGCAGAAGC TTATTCCTAC AGGCAGCCTG 
CCAGCAACTT TACAAGGAGC TACAGATGCT TTAGGATTGG AATGGCACCT CCCAAGTCCA 
GATCCCGTCA CTGTTCCGTA TCTCAGTCCT TTGGTGGTGT GGAAGGAACT TGAAAGCTTA 
TTGGAGAATG AAGGCGATCA TGCAATAACA GTGGCAGATT TTGTGGACCA TCATCCAATT 
GTTTTTTGGA ACCTAGTGTG GTATTTTAGA CGTCTTGACT TGCCCAGTAA CTTACCTGGA 
TTGATTCTTT CTTCTGAGCA TTGTAACAAA TATTCAAAGA TTCCCCGCCA CTGTATGTCT 
GAAGATAGTA AATATGTTTT AATACAAATG TTATGGGACA ATATGAAATT ACATCAGGAT 
CCGGGTCAGC CCTTGTACAT CCTCTGGAAT GCCCACAGTC AAAATCGTAC TCTTTTGTTT 
GAGACCCAAA AGTATCCAAT GGTCCATTTA TTGCAAAAGG GTGATAATTC ATTTAACCAA 
GAACTATTGA AAAATATGGT AAAAAGCATT AAAATGAATG ATGTCTATGG ACCAATGAGT 
CAGATTTTAG AGACACTGAA TAAATGTCCT CATTTTAAAA GACAGAGGAG TTTATATAGA 
GAAATACTCT TTCTCTCACT TGTGGCACTG GGAAGAGAAA ACATTGACAT AGATGCATTT 
GATAAAGAAT ACAAGATGGC ATACGATCGT CTCACCCCTA GTCAAGTCAA GAGTACACAC 
AACTGTGATA GACCACCAAG TACGGGGGTG ATGGAATGTC GGAAAACCTT TGGAGAACCT 
TATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_023655154.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023510922.1 Equus caballus DENN domain containing 4A (DENND4A), transcript variant X12, mRNA.

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CloneID	OHb41924
Clone ID Related Accession (Same CDS sequence)	XM_023655147.1 , XM_023655154.1
Accession Version	XM_023655154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCAA CTATTGAGTC TTGGCCACCA AATAGCAAAT ACCCTCTCCC TGTATTTTCT 
ACATTTGTGT TAACTGGAGC CTCAGCTGAA AAGGTCTATG GTGCTGCTAT TCAGTTTTAT 
GAACCATACT CTGAGGAGAA TCTCACAGAA AAACAGAGAC TTCTTTTGGG TTTAGCTTCA 
TCGGCAGATG GGAAGTCTGA TAGTTCCAAA ACAATTCACA CTAATAAATG CATCTGTCTT 
CTTTCTCACT GGCCTTTTTT TGATGCATTC AGGAAGTTTC TGACCTTTTT GTATCGTTAC 
TCCATCTCTG GACCTCATGT CCTTCCAATT GAGAAGCATA TTTCTCATTT TATGCATAAA 
GTTCCTTTTC CATCTCCTCA GAGGCCACGG ATTTTAGTTC AGTTATCACC GCATGATAAT 
CTGATTCTCA GTCAGCCTGT TTCTTCACCT CTTCCACTAA GTGGTGGCAA GTTTTCAACA 
CTGCTTCAGA ATTTAGGCCC TGAAAATGCT GTGACACTAC TGGTGTTTGC AGTAACAGAA 
CATAAGATTC TCATCCATTC CTTACGGCCT TCCGTGCTTA CTAGTGTGAC AGAAGCATTA 
GTGTCTATGA TTTTCCCTTT TCACTGGCCA TGCCCGTATG TTCCTCTGTG CCCGCTGGCT 
TTAGCAGATG TCTTGAGTGC ACCATGTCCA TTCATAGTAG GAATTGATTC AAGATACTTT 
GATCTCTATG ACCCTCCACC AGATGTTAGT TGTGTTGACC TGGATACCAA TACCATTTCT 
CAACCTGGTG ACAAGAAAAA TGTAGCCTGG AAGATACTTC CAAAGAAACC ATGTAAAAAT 
CTGATGAACA CACTGAATAA TTTGCACCAG CAATTGGCAA AATTACAGCA GAGACCAAGA 
GAAGATGGAC TAATGGACTT AGCGATGAAT GACTATGATT TTAATTCTGG AAAGAGGTTG 
CACATGATAG ATTTGGAAAT CCAAGAGGCA TTTTTGTGTT TCATGGCCTC TATTTTAAAA 
GGTTACAGAT CATACTTAAG ACCAATAACA CAGGCCCCAT CTGAGACAGC CACAGATGCA 
ACCTCTCTTT TTGCCCTGCA AGCTTTCTTA AGAAGCCGGG ACCGATCACA TCAAAAATTC 
TATAACATGA TGACCAAAAC ACAAATGTTT ATTCGCTTCA TTGAGGAATG TTCTTTTGTC 
AGTGATAAAG ATGCAAGCCT GGCATTTTTT GATGATTGTG TAGATAAAGT GGACATGGAC 
AAGAATGGTG AAATGCGACT TATAGAACTG GATGAATCTT TCAAAAGTGA ACATACTGTC 
TTTGTAACAC CTCCTGAGAT TCCCCATCTA CCCAATGGTG AAGAACCCCC TTTACAGTAC 
AGCTATAATG GATTTCCAGT TCTTAGGAGC AGCTTATTTG AAAGACCTGA AGGGTTTCTG 
CAGACACGAA AGAGCAAATT GCCTTCAAAA CCAAGTAGTC CTGGTAGCCC TTCTCCCGTG 
TTCCGAAGAA CAAAACAGGA AATTAAATCA GCCCATAAAA TTGCCAAGAG GTATTCTTCC 
ATTCCTCAGA TGTGGTCTAG GTGTCTACTG CGTCACTGTT ATGGACTGTG GTTTATTTGT 
CTCCCAGCTT ATGTGAAAGT ATGTCATTCA AAGGTCAGGG CTCTGAAAAC GGGATATGAT 
GTGCTTAAGA AAATGCAGTC AAAGAAGATG GATCCACCCG ATGAGGTGTG CTACCGCATT 
CTTATGCAGC TCTGTGGACA GTATGATCAG CCGGTGCTTG CAGTTCGAGT GCTTTTTGAA 
ATGCAGAAAG CTGGTATTGA CCCCAATGCC ATTACTTATG GTTATTATAA TAAGGCTGTT 
TTGGAAAGTA CATGGCCTTC AAGAAGTCGT AGTGGCTATT TTCTTTGGAC AAAAGTAAGA 
AATGTTGTTT TAGGAGTGGC ACAGTTCAAA AGAGCTTTAA AGAAACATGC GCATTTATCA 
CAAACAACTC TCTCAGCAGA TGGCAGTGAC CTGGATGCTG TTAGTCATGG CAGCATGGAT 
AGCGGTCATG GGACACACAC TGTGGAGCAG GCACCTTTTA ATACGGGTTT AATCAAAGTA 
TATGCTACTG ATGATAGATC TAGTACAGGT GGCCAGTCTG ATCTTGGATA TAACTCATTA 
TCTAAGGATG AAGTTAGAAG AGGACATACA TCTACTGAAG ACATTCAAGA AGAAAAAGAT 
AGGAAAGGGA GTGATTCTAG TTCTTTGTCA GAGAGTGAGA GTGCAAAAGG AAGTGCTGAT 
TGTCTGCCTA AGCTCAGTTT TCAAAGTTCT TCCAGTATAG TTCGCCTCAG TGGTGCAAAT 
AACAACAGTG CTGATAACAC CTCAGGGAAA AGCACAGAAA GCACATCTGA GCTGCTTGTA 
ATATCATCTT TTGAGGACAC AAATGAAACA GGAAACATTC AAAGTAGATG CTTCAGGAAA 
AGACATAAAA GTGACAATGA ACCTAATTTG CAGCAGCAAA TGTCCTGGGG AAGTAGAAAC 
CGCAATCTTA GTGGAGGAGT ACTGATGGGA CTTATGCTCA ATAGGATGAA CCAGGAAGCA 
AACCCTGGTG ATATGGTTGA AAAATTAGGA GCTGATGCAA AAATTCTTTC AAATGTTATC 
TCAAAAAGCA CAAGACCAAA TAGTCTTGAC ATTGGAAAGC CACCTGTAAG ATCAAAAAGA 
GACAGTCTAG AGAAGGAATC TAGTGATGAT GATACACCTT TTGATGGTTC TAACTGTTTG 
GCAGATAAAG TGGATTCTCC CGTTATTTTT GACTTAGAAG ACTTAGACAA TGAAACAGAT 
GGATCAAAAG TGGGATATGT TGCTACACAA AATCCCAAGA GGATTCAGCG TATGAGCAGC 
AGCTTTTCAG TAAAACCTTT TGAAAAAACT GATGTTGCAA CAGGATTTGA TCCCCTCTCT 
CTTTTGGTTG CTGAGACTGA ACAGCAGCAA AAAGAGGAGG AGGAAGAGGG GGAAGATGAT 
AGCAAAAGCA TTTCAACACC ATCTGCCAGA CGTGATTTAG CTGAAGAAAT TGAGATGTAT 
ATGAATAACA TGAGCAGTCC TTTGACAAGT CGTACACCAA GCATTGATTT ACAACGGGCA 
TGTGATGATA AATTAACCAA TAAGAAAAGT CCAACATTGG TCAAGGCATG CAGAAGATCT 
AGCCTGCCTC CTAATTCTCC TAGGCCAGTG AGACTTACCA AATCTAAAAG TTACACAAAA 
AGTGAGGACA GGCCAAGGGA TAGACTCTGG TCTTCTCCAG CCTTCTCACC CACTTGCCCT 
TTTAGGGAAG AATCTCAAGA TGCATTACCC CACTCATCAC CTTCATTTAA TTTGGATACA 
CTACTAGTAC CTAAACTGGA TGTTTTAAGG AACAGCATGT TCACTGCTGG AAAAGGAGTA 
GCCGAGAAAG CAAGCAAATG GTATTCAAGG TTCACCATGT ATACTACATC ATCTAAGGAT 
CAAAGTTCTG ATCGTGCCAG TCTTTCTTCA GTGGGAGCCC AGGATTCTGA ATCTGCCTCT 
TTAACAGATG AAGATGTCTG CCACGAGCTG GAAGGAGCCG CTTCCTCCCA AGAGAGCAGT 
GCCACTTCAG GGACCAAGGG AATTGATTTC AGCAGAACAA GCCTGGGAAG TTCTGCCTCC 
TTAGAAGGAT CCCTGTCAAA GTTCGCCTTA CCTGGGAAAT CAGAAGTGGC ATCTTCCTAC 
AACACGAGTA ATACAAATAT CTTCCAGAAC TATGCAATGG AGGTTCTCAT CTCAAGTTGC 
TCTCGGTGTA GAACTTGTGA CTGCCTTGTC CATGATGAGG AAATCATGGC TGGCTGGACA 
GCAGATGACT CAAATCTCAA TACCACATGC CCGTTTTGTG GCAATCTTTT CTTGCCCTTT 
TTGAATATTG AAATAAGAGA TTTAAGACGA CCCGGAAAAT ACTTTTTGAA GTCAAGCCCA 
TCTGCAGAAA ATATGCATTT TCCGTCTTCC TTTTCAAGTC AGACAAGACA GTCTTGTATC 
TCAACATCAG CCTCTGGTCT TGACAAGTCT GTTATCTCCG TTCCAGGGAA TTTTGATCTA 
CATAGCAAAT CTAAACTGCT GGAAAATTCA TGTGCCCGAA GTATTCAGAT CCCTGCTGAT 
AGATCAAAAA CAGCTGTGTC AAAATGTCCA GTATTTCCAA TGGCCAGGAG CGTCAGTACT 
TATGGCCCCT TGGATAAAGA AGACACTGGA GGGCAGAAGC TTATTCCTAC AGGCAGCCTG 
CCAGCAACTT TACAAGGAGC TACAGATGCT TTAGGATTGG AATGGCACCT CCCAAGTCCA 
GATCCCGTCA CTGTTCCGTA TCTCAGTCCT TTGGTGGTGT GGAAGGAACT TGAAAGCTTA 
TTGGAGAATG AAGGCGATCA TGCAATAACA GTGGCAGATT TTGTGGACCA TCATCCAATT 
GTTTTTTGGA ACCTAGTGTG GTATTTTAGA CGTCTTGACT TGCCCAGTAA CTTACCTGGA 
TTGATTCTTT CTTCTGAGCA TTGTAACAAA TATTCAAAGA TTCCCCGCCA CTGTATGTCT 
GAAGATAGTA AATATGTTTT AATACAAATG TTATGGGACA ATATGAAATT ACATCAGGAT 
CCGGGTCAGC CCTTGTACAT CCTCTGGAAT GCCCACAGTC AAAATCGTAC TCTTTTGTTT 
GAGACCCAAA AGTATCCAAT GGTCCATTTA TTGCAAAAGG GTGATAATTC ATTTAACCAA 
GAACTATTGA AAAATATGGT AAAAAGCATT AAAATGAATG ATGTCTATGG ACCAATGAGT 
CAGATTTTAG AGACACTGAA TAAATGTCCT CATTTTAAAA GACAGAGGAG TTTATATAGA 
GAAATACTCT TTCTCTCACT TGTGGCACTG GGAAGAGAAA ACATTGACAT AGATGCATTT 
GATAAAGAAT ACAAGATGGC ATACGATCGT CTCACCCCTA GTCAAGTCAA GAGTACACAC 
AACTGTGATA GACCACCAAG TACGGGGGTG ATGGAATGTC GGAAAACCTT TGGAGAACCT 
TATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023510922.1
CDS	365..5413
Translation	MGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_023655154.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023655154.1

ORF Insert Sequence:

ATGGGTGCAA CTATTGAGTC TTGGCCACCA AATAGCAAAT ACCCTCTCCC TGTATTTTCT 
ACATTTGTGT TAACTGGAGC CTCAGCTGAA AAGGTCTATG GTGCTGCTAT TCAGTTTTAT 
GAACCATACT CTGAGGAGAA TCTCACAGAA AAACAGAGAC TTCTTTTGGG TTTAGCTTCA 
TCGGCAGATG GGAAGTCTGA TAGTTCCAAA ACAATTCACA CTAATAAATG CATCTGTCTT 
CTTTCTCACT GGCCTTTTTT TGATGCATTC AGGAAGTTTC TGACCTTTTT GTATCGTTAC 
TCCATCTCTG GACCTCATGT CCTTCCAATT GAGAAGCATA TTTCTCATTT TATGCATAAA 
GTTCCTTTTC CATCTCCTCA GAGGCCACGG ATTTTAGTTC AGTTATCACC GCATGATAAT 
CTGATTCTCA GTCAGCCTGT TTCTTCACCT CTTCCACTAA GTGGTGGCAA GTTTTCAACA 
CTGCTTCAGA ATTTAGGCCC TGAAAATGCT GTGACACTAC TGGTGTTTGC AGTAACAGAA 
CATAAGATTC TCATCCATTC CTTACGGCCT TCCGTGCTTA CTAGTGTGAC AGAAGCATTA 
GTGTCTATGA TTTTCCCTTT TCACTGGCCA TGCCCGTATG TTCCTCTGTG CCCGCTGGCT 
TTAGCAGATG TCTTGAGTGC ACCATGTCCA TTCATAGTAG GAATTGATTC AAGATACTTT 
GATCTCTATG ACCCTCCACC AGATGTTAGT TGTGTTGACC TGGATACCAA TACCATTTCT 
CAACCTGGTG ACAAGAAAAA TGTAGCCTGG AAGATACTTC CAAAGAAACC ATGTAAAAAT 
CTGATGAACA CACTGAATAA TTTGCACCAG CAATTGGCAA AATTACAGCA GAGACCAAGA 
GAAGATGGAC TAATGGACTT AGCGATGAAT GACTATGATT TTAATTCTGG AAAGAGGTTG 
CACATGATAG ATTTGGAAAT CCAAGAGGCA TTTTTGTGTT TCATGGCCTC TATTTTAAAA 
GGTTACAGAT CATACTTAAG ACCAATAACA CAGGCCCCAT CTGAGACAGC CACAGATGCA 
ACCTCTCTTT TTGCCCTGCA AGCTTTCTTA AGAAGCCGGG ACCGATCACA TCAAAAATTC 
TATAACATGA TGACCAAAAC ACAAATGTTT ATTCGCTTCA TTGAGGAATG TTCTTTTGTC 
AGTGATAAAG ATGCAAGCCT GGCATTTTTT GATGATTGTG TAGATAAAGT GGACATGGAC 
AAGAATGGTG AAATGCGACT TATAGAACTG GATGAATCTT TCAAAAGTGA ACATACTGTC 
TTTGTAACAC CTCCTGAGAT TCCCCATCTA CCCAATGGTG AAGAACCCCC TTTACAGTAC 
AGCTATAATG GATTTCCAGT TCTTAGGAGC AGCTTATTTG AAAGACCTGA AGGGTTTCTG 
CAGACACGAA AGAGCAAATT GCCTTCAAAA CCAAGTAGTC CTGGTAGCCC TTCTCCCGTG 
TTCCGAAGAA CAAAACAGGA AATTAAATCA GCCCATAAAA TTGCCAAGAG GTATTCTTCC 
ATTCCTCAGA TGTGGTCTAG GTGTCTACTG CGTCACTGTT ATGGACTGTG GTTTATTTGT 
CTCCCAGCTT ATGTGAAAGT ATGTCATTCA AAGGTCAGGG CTCTGAAAAC GGGATATGAT 
GTGCTTAAGA AAATGCAGTC AAAGAAGATG GATCCACCCG ATGAGGTGTG CTACCGCATT 
CTTATGCAGC TCTGTGGACA GTATGATCAG CCGGTGCTTG CAGTTCGAGT GCTTTTTGAA 
ATGCAGAAAG CTGGTATTGA CCCCAATGCC ATTACTTATG GTTATTATAA TAAGGCTGTT 
TTGGAAAGTA CATGGCCTTC AAGAAGTCGT AGTGGCTATT TTCTTTGGAC AAAAGTAAGA 
AATGTTGTTT TAGGAGTGGC ACAGTTCAAA AGAGCTTTAA AGAAACATGC GCATTTATCA 
CAAACAACTC TCTCAGCAGA TGGCAGTGAC CTGGATGCTG TTAGTCATGG CAGCATGGAT 
AGCGGTCATG GGACACACAC TGTGGAGCAG GCACCTTTTA ATACGGGTTT AATCAAAGTA 
TATGCTACTG ATGATAGATC TAGTACAGGT GGCCAGTCTG ATCTTGGATA TAACTCATTA 
TCTAAGGATG AAGTTAGAAG AGGACATACA TCTACTGAAG ACATTCAAGA AGAAAAAGAT 
AGGAAAGGGA GTGATTCTAG TTCTTTGTCA GAGAGTGAGA GTGCAAAAGG AAGTGCTGAT 
TGTCTGCCTA AGCTCAGTTT TCAAAGTTCT TCCAGTATAG TTCGCCTCAG TGGTGCAAAT 
AACAACAGTG CTGATAACAC CTCAGGGAAA AGCACAGAAA GCACATCTGA GCTGCTTGTA 
ATATCATCTT TTGAGGACAC AAATGAAACA GGAAACATTC AAAGTAGATG CTTCAGGAAA 
AGACATAAAA GTGACAATGA ACCTAATTTG CAGCAGCAAA TGTCCTGGGG AAGTAGAAAC 
CGCAATCTTA GTGGAGGAGT ACTGATGGGA CTTATGCTCA ATAGGATGAA CCAGGAAGCA 
AACCCTGGTG ATATGGTTGA AAAATTAGGA GCTGATGCAA AAATTCTTTC AAATGTTATC 
TCAAAAAGCA CAAGACCAAA TAGTCTTGAC ATTGGAAAGC CACCTGTAAG ATCAAAAAGA 
GACAGTCTAG AGAAGGAATC TAGTGATGAT GATACACCTT TTGATGGTTC TAACTGTTTG 
GCAGATAAAG TGGATTCTCC CGTTATTTTT GACTTAGAAG ACTTAGACAA TGAAACAGAT 
GGATCAAAAG TGGGATATGT TGCTACACAA AATCCCAAGA GGATTCAGCG TATGAGCAGC 
AGCTTTTCAG TAAAACCTTT TGAAAAAACT GATGTTGCAA CAGGATTTGA TCCCCTCTCT 
CTTTTGGTTG CTGAGACTGA ACAGCAGCAA AAAGAGGAGG AGGAAGAGGG GGAAGATGAT 
AGCAAAAGCA TTTCAACACC ATCTGCCAGA CGTGATTTAG CTGAAGAAAT TGAGATGTAT 
ATGAATAACA TGAGCAGTCC TTTGACAAGT CGTACACCAA GCATTGATTT ACAACGGGCA 
TGTGATGATA AATTAACCAA TAAGAAAAGT CCAACATTGG TCAAGGCATG CAGAAGATCT 
AGCCTGCCTC CTAATTCTCC TAGGCCAGTG AGACTTACCA AATCTAAAAG TTACACAAAA 
AGTGAGGACA GGCCAAGGGA TAGACTCTGG TCTTCTCCAG CCTTCTCACC CACTTGCCCT 
TTTAGGGAAG AATCTCAAGA TGCATTACCC CACTCATCAC CTTCATTTAA TTTGGATACA 
CTACTAGTAC CTAAACTGGA TGTTTTAAGG AACAGCATGT TCACTGCTGG AAAAGGAGTA 
GCCGAGAAAG CAAGCAAATG GTATTCAAGG TTCACCATGT ATACTACATC ATCTAAGGAT 
CAAAGTTCTG ATCGTGCCAG TCTTTCTTCA GTGGGAGCCC AGGATTCTGA ATCTGCCTCT 
TTAACAGATG AAGATGTCTG CCACGAGCTG GAAGGAGCCG CTTCCTCCCA AGAGAGCAGT 
GCCACTTCAG GGACCAAGGG AATTGATTTC AGCAGAACAA GCCTGGGAAG TTCTGCCTCC 
TTAGAAGGAT CCCTGTCAAA GTTCGCCTTA CCTGGGAAAT CAGAAGTGGC ATCTTCCTAC 
AACACGAGTA ATACAAATAT CTTCCAGAAC TATGCAATGG AGGTTCTCAT CTCAAGTTGC 
TCTCGGTGTA GAACTTGTGA CTGCCTTGTC CATGATGAGG AAATCATGGC TGGCTGGACA 
GCAGATGACT CAAATCTCAA TACCACATGC CCGTTTTGTG GCAATCTTTT CTTGCCCTTT 
TTGAATATTG AAATAAGAGA TTTAAGACGA CCCGGAAAAT ACTTTTTGAA GTCAAGCCCA 
TCTGCAGAAA ATATGCATTT TCCGTCTTCC TTTTCAAGTC AGACAAGACA GTCTTGTATC 
TCAACATCAG CCTCTGGTCT TGACAAGTCT GTTATCTCCG TTCCAGGGAA TTTTGATCTA 
CATAGCAAAT CTAAACTGCT GGAAAATTCA TGTGCCCGAA GTATTCAGAT CCCTGCTGAT 
AGATCAAAAA CAGCTGTGTC AAAATGTCCA GTATTTCCAA TGGCCAGGAG CGTCAGTACT 
TATGGCCCCT TGGATAAAGA AGACACTGGA GGGCAGAAGC TTATTCCTAC AGGCAGCCTG 
CCAGCAACTT TACAAGGAGC TACAGATGCT TTAGGATTGG AATGGCACCT CCCAAGTCCA 
GATCCCGTCA CTGTTCCGTA TCTCAGTCCT TTGGTGGTGT GGAAGGAACT TGAAAGCTTA 
TTGGAGAATG AAGGCGATCA TGCAATAACA GTGGCAGATT TTGTGGACCA TCATCCAATT 
GTTTTTTGGA ACCTAGTGTG GTATTTTAGA CGTCTTGACT TGCCCAGTAA CTTACCTGGA 
TTGATTCTTT CTTCTGAGCA TTGTAACAAA TATTCAAAGA TTCCCCGCCA CTGTATGTCT 
GAAGATAGTA AATATGTTTT AATACAAATG TTATGGGACA ATATGAAATT ACATCAGGAT 
CCGGGTCAGC CCTTGTACAT CCTCTGGAAT GCCCACAGTC AAAATCGTAC TCTTTTGTTT 
GAGACCCAAA AGTATCCAAT GGTCCATTTA TTGCAAAAGG GTGATAATTC ATTTAACCAA 
GAACTATTGA AAAATATGGT AAAAAGCATT AAAATGAATG ATGTCTATGG ACCAATGAGT 
CAGATTTTAG AGACACTGAA TAAATGTCCT CATTTTAAAA GACAGAGGAG TTTATATAGA 
GAAATACTCT TTCTCTCACT TGTGGCACTG GGAAGAGAAA ACATTGACAT AGATGCATTT 
GATAAAGAAT ACAAGATGGC ATACGATCGT CTCACCCCTA GTCAAGTCAA GAGTACACAC 
AACTGTGATA GACCACCAAG TACGGGGGTG ATGGAATGTC GGAAAACCTT TGGAGAACCT 
TATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_023655111.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023510879.1 Equus caballus DENN domain containing 4A (DENND4A), transcript variant X7, mRNA.

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CloneID	OHb10409
Clone ID Related Accession (Same CDS sequence)	XM_023655111.1 , XM_005602983.2
Accession Version	XM_023655111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGCGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAACT CATTATCTAA GGATGAAGTT 
AGAAGAGGAC ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAGGAA AGGGAGTGAT 
TCTAGTTCTT TGTCAGAGAG TGAGAGTGCA AAAGGAAGTG CTGATTGTCT GCCTAAGCTC 
AGTTTTCAAA GTTCTTCCAG TATAGTTCGC CTCAGTGGTG CAAATAACAA CAGTGCTGAT 
AACACCTCAG GGAAAAGCAC AGAAAGCACA TCTGAGCTGC TTGTAATATC ATCTTTTGAG 
GACACAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAACCTA ATTTGCAGCA GCAAATGTCC TGGGGAAGTA GAAACCGCAA TCTTAGTGGA 
GGAGTACTGA TGGGACTTAT GCTCAATAGG ATGAACCAGG AAGCAAACCC TGGTGATATG 
GTTGAAAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACAAGA 
CCAAATAGTC TTGACATTGG AAAGCCACCT GTAAGATCAA AAAGAGACAG TCTAGAGAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT GTTTGGCAGA TAAAGTGGAT 
TCTCCCGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATGGATC AAAAGTGGGA 
TATGTTGCTA CACAAAATCC CAAGAGGATT CAGCGTATGA GCAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT TGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGGGGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CCAGACGTGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
ACCAATAAGA AAAGTCCAAC ATTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCTAGGC CAGTGAGACT TACCAAATCT AAAAGTTACA CAAAAAGTGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCCACTT GCCCTTTTAG GGAAGAATCT 
CAAGATGCAT TACCCCACTC ATCACCTTCA TTTAATTTGG ATACACTACT AGTACCTAAA 
CTGGATGTTT TAAGGAACAG CATGTTCACT GCTGGAAAAG GAGTAGCCGA GAAAGCAAGC 
AAATGGTATT CAAGGTTCAC CATGTATACT ACATCATCTA AGGATCAAAG TTCTGATCGT 
GCCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTG CCTCTTTAAC AGATGAAGAT 
GTCTGCCACG AGCTGGAAGG AGCCGCTTCC TCCCAAGAGA GCAGTGCCAC TTCAGGGACC 
AAGGGAATTG ATTTCAGCAG AACAAGCCTG GGAAGTTCTG CCTCCTTAGA AGGATCCCTG 
TCAAAGTTCG CCTTACCTGG GAAATCAGAA GTGGCATCTT CCTACAACAC GAGTAATACA 
AATATCTTCC AGAACTATGC AATGGAGGTT CTCATCTCAA GTTGCTCTCG GTGTAGAACT 
TGTGACTGCC TTGTCCATGA TGAGGAAATC ATGGCTGGCT GGACAGCAGA TGACTCAAAT 
CTCAATACCA CATGCCCGTT TTGTGGCAAT CTTTTCTTGC CCTTTTTGAA TATTGAAATA 
AGAGATTTAA GACGACCCGG AAAATACTTT TTGAAGTCAA GCCCATCTGC AGAAAATATG 
CATTTTCCGT CTTCCTTTTC AAGTCAGACA AGACAGTCTT GTATCTCAAC ATCAGCCTCT 
GGTCTTGACA AGTCTGTTAT CTCCGTTCCA GGGAATTTTG ATCTACATAG CAAATCTAAA 
CTGCTGGAAA ATTCATGTGC CCGAAGTATT CAGATCCCTG CTGATAGATC AAAAACAGCT 
GTGTCAAAAT GTCCAGTATT TCCAATGGCC AGGAGCGTCA GTACTTATGG CCCCTTGGAT 
AAAGAAGACA CTGGAGGGCA GAAGCTTATT CCTACAGGCA GCCTGCCAGC AACTTTACAA 
GGAGCTACAG ATGCTTTAGG ATTGGAATGG CACCTCCCAA GTCCAGATCC CGTCACTGTT 
CCGTATCTCA GTCCTTTGGT GGTGTGGAAG GAACTTGAAA GCTTATTGGA GAATGAAGGC 
GATCATGCAA TAACAGTGGC AGATTTTGTG GACCATCATC CAATTGTTTT TTGGAACCTA 
GTGTGGTATT TTAGACGTCT TGACTTGCCC AGTAACTTAC CTGGATTGAT TCTTTCTTCT 
GAGCATTGTA ACAAATATTC AAAGATTCCC CGCCACTGTA TGTCTGAAGA TAGTAAATAT 
GTTTTAATAC AAATGTTATG GGACAATATG AAATTACATC AGGATCCGGG TCAGCCCTTG 
TACATCCTCT GGAATGCCCA CACCCAAAAG TATCCAATGG TCCATTTATT GCAAAAGGGT 
GATAATTCAT TTAACCAAGA ACTATTGAAA AATATGGTAA AAAGCATTAA AATGAATGAT 
GTCTATGGAC CAATGAGTCA GATTTTAGAG ACACTGAATA AATGTCCTCA TTTTAAAAGA 
CAGAGGAGTT TATATAGAGA AATACTCTTT CTCTCACTTG TGGCACTGGG AAGAGAAAAC 
ATTGACATAG ATGCATTTGA TAAAGAATAC AAGATGGCAT ACGATCGTCT CACCCCTAGT 
CAAGTCAAGA GTACACACAA CTGTGATAGA CCACCAAGTA CGGGGGTGAT GGAATGTCGG 
AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023510879.1
CDS	584..6310
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVVIKSLGEEVPRDYMCIDLTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGPAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSDGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_023655111.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023655111.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGCGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAACT CATTATCTAA GGATGAAGTT 
AGAAGAGGAC ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAGGAA AGGGAGTGAT 
TCTAGTTCTT TGTCAGAGAG TGAGAGTGCA AAAGGAAGTG CTGATTGTCT GCCTAAGCTC 
AGTTTTCAAA GTTCTTCCAG TATAGTTCGC CTCAGTGGTG CAAATAACAA CAGTGCTGAT 
AACACCTCAG GGAAAAGCAC AGAAAGCACA TCTGAGCTGC TTGTAATATC ATCTTTTGAG 
GACACAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAACCTA ATTTGCAGCA GCAAATGTCC TGGGGAAGTA GAAACCGCAA TCTTAGTGGA 
GGAGTACTGA TGGGACTTAT GCTCAATAGG ATGAACCAGG AAGCAAACCC TGGTGATATG 
GTTGAAAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACAAGA 
CCAAATAGTC TTGACATTGG AAAGCCACCT GTAAGATCAA AAAGAGACAG TCTAGAGAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT GTTTGGCAGA TAAAGTGGAT 
TCTCCCGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATGGATC AAAAGTGGGA 
TATGTTGCTA CACAAAATCC CAAGAGGATT CAGCGTATGA GCAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT TGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGGGGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CCAGACGTGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
ACCAATAAGA AAAGTCCAAC ATTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCTAGGC CAGTGAGACT TACCAAATCT AAAAGTTACA CAAAAAGTGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCCACTT GCCCTTTTAG GGAAGAATCT 
CAAGATGCAT TACCCCACTC ATCACCTTCA TTTAATTTGG ATACACTACT AGTACCTAAA 
CTGGATGTTT TAAGGAACAG CATGTTCACT GCTGGAAAAG GAGTAGCCGA GAAAGCAAGC 
AAATGGTATT CAAGGTTCAC CATGTATACT ACATCATCTA AGGATCAAAG TTCTGATCGT 
GCCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTG CCTCTTTAAC AGATGAAGAT 
GTCTGCCACG AGCTGGAAGG AGCCGCTTCC TCCCAAGAGA GCAGTGCCAC TTCAGGGACC 
AAGGGAATTG ATTTCAGCAG AACAAGCCTG GGAAGTTCTG CCTCCTTAGA AGGATCCCTG 
TCAAAGTTCG CCTTACCTGG GAAATCAGAA GTGGCATCTT CCTACAACAC GAGTAATACA 
AATATCTTCC AGAACTATGC AATGGAGGTT CTCATCTCAA GTTGCTCTCG GTGTAGAACT 
TGTGACTGCC TTGTCCATGA TGAGGAAATC ATGGCTGGCT GGACAGCAGA TGACTCAAAT 
CTCAATACCA CATGCCCGTT TTGTGGCAAT CTTTTCTTGC CCTTTTTGAA TATTGAAATA 
AGAGATTTAA GACGACCCGG AAAATACTTT TTGAAGTCAA GCCCATCTGC AGAAAATATG 
CATTTTCCGT CTTCCTTTTC AAGTCAGACA AGACAGTCTT GTATCTCAAC ATCAGCCTCT 
GGTCTTGACA AGTCTGTTAT CTCCGTTCCA GGGAATTTTG ATCTACATAG CAAATCTAAA 
CTGCTGGAAA ATTCATGTGC CCGAAGTATT CAGATCCCTG CTGATAGATC AAAAACAGCT 
GTGTCAAAAT GTCCAGTATT TCCAATGGCC AGGAGCGTCA GTACTTATGG CCCCTTGGAT 
AAAGAAGACA CTGGAGGGCA GAAGCTTATT CCTACAGGCA GCCTGCCAGC AACTTTACAA 
GGAGCTACAG ATGCTTTAGG ATTGGAATGG CACCTCCCAA GTCCAGATCC CGTCACTGTT 
CCGTATCTCA GTCCTTTGGT GGTGTGGAAG GAACTTGAAA GCTTATTGGA GAATGAAGGC 
GATCATGCAA TAACAGTGGC AGATTTTGTG GACCATCATC CAATTGTTTT TTGGAACCTA 
GTGTGGTATT TTAGACGTCT TGACTTGCCC AGTAACTTAC CTGGATTGAT TCTTTCTTCT 
GAGCATTGTA ACAAATATTC AAAGATTCCC CGCCACTGTA TGTCTGAAGA TAGTAAATAT 
GTTTTAATAC AAATGTTATG GGACAATATG AAATTACATC AGGATCCGGG TCAGCCCTTG 
TACATCCTCT GGAATGCCCA CACCCAAAAG TATCCAATGG TCCATTTATT GCAAAAGGGT 
GATAATTCAT TTAACCAAGA ACTATTGAAA AATATGGTAA AAAGCATTAA AATGAATGAT 
GTCTATGGAC CAATGAGTCA GATTTTAGAG ACACTGAATA AATGTCCTCA TTTTAAAAGA 
CAGAGGAGTT TATATAGAGA AATACTCTTT CTCTCACTTG TGGCACTGGG AAGAGAAAAC 
ATTGACATAG ATGCATTTGA TAAAGAATAC AAGATGGCAT ACGATCGTCT CACCCCTAGT 
CAAGTCAAGA GTACACACAA CTGTGATAGA CCACCAAGTA CGGGGGTGAT GGAATGTCGG 
AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005602983.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_005603040.1 Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X4, mRNA.

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CloneID	OHb10409
Clone ID Related Accession (Same CDS sequence)	XM_023655111.1 , XM_005602983.2
Accession Version	XM_005602983.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867387.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005602983.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGCGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAACT CATTATCTAA GGATGAAGTT 
AGAAGAGGAC ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAGGAA AGGGAGTGAT 
TCTAGTTCTT TGTCAGAGAG TGAGAGTGCA AAAGGAAGTG CTGATTGTCT GCCTAAGCTC 
AGTTTTCAAA GTTCTTCCAG TATAGTTCGC CTCAGTGGTG CAAATAACAA CAGTGCTGAT 
AACACCTCAG GGAAAAGCAC AGAAAGCACA TCTGAGCTGC TTGTAATATC ATCTTTTGAG 
GACACAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAACCTA ATTTGCAGCA GCAAATGTCC TGGGGAAGTA GAAACCGCAA TCTTAGTGGA 
GGAGTACTGA TGGGACTTAT GCTCAATAGG ATGAACCAGG AAGCAAACCC TGGTGATATG 
GTTGAAAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACAAGA 
CCAAATAGTC TTGACATTGG AAAGCCACCT GTAAGATCAA AAAGAGACAG TCTAGAGAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT GTTTGGCAGA TAAAGTGGAT 
TCTCCCGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATGGATC AAAAGTGGGA 
TATGTTGCTA CACAAAATCC CAAGAGGATT CAGCGTATGA GCAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT TGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGGGGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CCAGACGTGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
ACCAATAAGA AAAGTCCAAC ATTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCTAGGC CAGTGAGACT TACCAAATCT AAAAGTTACA CAAAAAGTGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCCACTT GCCCTTTTAG GGAAGAATCT 
CAAGATGCAT TACCCCACTC ATCACCTTCA TTTAATTTGG ATACACTACT AGTACCTAAA 
CTGGATGTTT TAAGGAACAG CATGTTCACT GCTGGAAAAG GAGTAGCCGA GAAAGCAAGC 
AAATGGTATT CAAGGTTCAC CATGTATACT ACATCATCTA AGGATCAAAG TTCTGATCGT 
GCCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTG CCTCTTTAAC AGATGAAGAT 
GTCTGCCACG AGCTGGAAGG AGCCGCTTCC TCCCAAGAGA GCAGTGCCAC TTCAGGGACC 
AAGGGAATTG ATTTCAGCAG AACAAGCCTG GGAAGTTCTG CCTCCTTAGA AGGATCCCTG 
TCAAAGTTCG CCTTACCTGG GAAATCAGAA GTGGCATCTT CCTACAACAC GAGTAATACA 
AATATCTTCC AGAACTATGC AATGGAGGTT CTCATCTCAA GTTGCTCTCG GTGTAGAACT 
TGTGACTGCC TTGTCCATGA TGAGGAAATC ATGGCTGGCT GGACAGCAGA TGACTCAAAT 
CTCAATACCA CATGCCCGTT TTGTGGCAAT CTTTTCTTGC CCTTTTTGAA TATTGAAATA 
AGAGATTTAA GACGACCCGG AAAATACTTT TTGAAGTCAA GCCCATCTGC AGAAAATATG 
CATTTTCCGT CTTCCTTTTC AAGTCAGACA AGACAGTCTT GTATCTCAAC ATCAGCCTCT 
GGTCTTGACA AGTCTGTTAT CTCCGTTCCA GGGAATTTTG ATCTACATAG CAAATCTAAA 
CTGCTGGAAA ATTCATGTGC CCGAAGTATT CAGATCCCTG CTGATAGATC AAAAACAGCT 
GTGTCAAAAT GTCCAGTATT TCCAATGGCC AGGAGCGTCA GTACTTATGG CCCCTTGGAT 
AAAGAAGACA CTGGAGGGCA GAAGCTTATT CCTACAGGCA GCCTGCCAGC AACTTTACAA 
GGAGCTACAG ATGCTTTAGG ATTGGAATGG CACCTCCCAA GTCCAGATCC CGTCACTGTT 
CCGTATCTCA GTCCTTTGGT GGTGTGGAAG GAACTTGAAA GCTTATTGGA GAATGAAGGC 
GATCATGCAA TAACAGTGGC AGATTTTGTG GACCATCATC CAATTGTTTT TTGGAACCTA 
GTGTGGTATT TTAGACGTCT TGACTTGCCC AGTAACTTAC CTGGATTGAT TCTTTCTTCT 
GAGCATTGTA ACAAATATTC AAAGATTCCC CGCCACTGTA TGTCTGAAGA TAGTAAATAT 
GTTTTAATAC AAATGTTATG GGACAATATG AAATTACATC AGGATCCGGG TCAGCCCTTG 
TACATCCTCT GGAATGCCCA CACCCAAAAG TATCCAATGG TCCATTTATT GCAAAAGGGT 
GATAATTCAT TTAACCAAGA ACTATTGAAA AATATGGTAA AAAGCATTAA AATGAATGAT 
GTCTATGGAC CAATGAGTCA GATTTTAGAG ACACTGAATA AATGTCCTCA TTTTAAAAGA 
CAGAGGAGTT TATATAGAGA AATACTCTTT CTCTCACTTG TGGCACTGGG AAGAGAAAAC 
ATTGACATAG ATGCATTTGA TAAAGAATAC AAGATGGCAT ACGATCGTCT CACCCCTAGT 
CAAGTCAAGA GTACACACAA CTGTGATAGA CCACCAAGTA CGGGGGTGAT GGAATGTCGG 
AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005603040.1
CDS	344..6070
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVVIKSLGEEVPRDYMCIDLTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGPAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSDGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005602983.2
Organism	Equus caballus(horse)
Definition	Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005602983.2

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGCGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAACT CATTATCTAA GGATGAAGTT 
AGAAGAGGAC ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAGGAA AGGGAGTGAT 
TCTAGTTCTT TGTCAGAGAG TGAGAGTGCA AAAGGAAGTG CTGATTGTCT GCCTAAGCTC 
AGTTTTCAAA GTTCTTCCAG TATAGTTCGC CTCAGTGGTG CAAATAACAA CAGTGCTGAT 
AACACCTCAG GGAAAAGCAC AGAAAGCACA TCTGAGCTGC TTGTAATATC ATCTTTTGAG 
GACACAAATG AAACAGGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAACCTA ATTTGCAGCA GCAAATGTCC TGGGGAAGTA GAAACCGCAA TCTTAGTGGA 
GGAGTACTGA TGGGACTTAT GCTCAATAGG ATGAACCAGG AAGCAAACCC TGGTGATATG 
GTTGAAAAAT TAGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACAAGA 
CCAAATAGTC TTGACATTGG AAAGCCACCT GTAAGATCAA AAAGAGACAG TCTAGAGAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT GTTTGGCAGA TAAAGTGGAT 
TCTCCCGTTA TTTTTGACTT AGAAGACTTA GACAATGAAA CAGATGGATC AAAAGTGGGA 
TATGTTGCTA CACAAAATCC CAAGAGGATT CAGCGTATGA GCAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT TGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAGGAGGAA GAGGGGGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CCAGACGTGA TTTAGCTGAA GAAATTGAGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
ACCAATAAGA AAAGTCCAAC ATTGGTCAAG GCATGCAGAA GATCTAGCCT GCCTCCTAAT 
TCTCCTAGGC CAGTGAGACT TACCAAATCT AAAAGTTACA CAAAAAGTGA GGACAGGCCA 
AGGGATAGAC TCTGGTCTTC TCCAGCCTTC TCACCCACTT GCCCTTTTAG GGAAGAATCT 
CAAGATGCAT TACCCCACTC ATCACCTTCA TTTAATTTGG ATACACTACT AGTACCTAAA 
CTGGATGTTT TAAGGAACAG CATGTTCACT GCTGGAAAAG GAGTAGCCGA GAAAGCAAGC 
AAATGGTATT CAAGGTTCAC CATGTATACT ACATCATCTA AGGATCAAAG TTCTGATCGT 
GCCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTG CCTCTTTAAC AGATGAAGAT 
GTCTGCCACG AGCTGGAAGG AGCCGCTTCC TCCCAAGAGA GCAGTGCCAC TTCAGGGACC 
AAGGGAATTG ATTTCAGCAG AACAAGCCTG GGAAGTTCTG CCTCCTTAGA AGGATCCCTG 
TCAAAGTTCG CCTTACCTGG GAAATCAGAA GTGGCATCTT CCTACAACAC GAGTAATACA 
AATATCTTCC AGAACTATGC AATGGAGGTT CTCATCTCAA GTTGCTCTCG GTGTAGAACT 
TGTGACTGCC TTGTCCATGA TGAGGAAATC ATGGCTGGCT GGACAGCAGA TGACTCAAAT 
CTCAATACCA CATGCCCGTT TTGTGGCAAT CTTTTCTTGC CCTTTTTGAA TATTGAAATA 
AGAGATTTAA GACGACCCGG AAAATACTTT TTGAAGTCAA GCCCATCTGC AGAAAATATG 
CATTTTCCGT CTTCCTTTTC AAGTCAGACA AGACAGTCTT GTATCTCAAC ATCAGCCTCT 
GGTCTTGACA AGTCTGTTAT CTCCGTTCCA GGGAATTTTG ATCTACATAG CAAATCTAAA 
CTGCTGGAAA ATTCATGTGC CCGAAGTATT CAGATCCCTG CTGATAGATC AAAAACAGCT 
GTGTCAAAAT GTCCAGTATT TCCAATGGCC AGGAGCGTCA GTACTTATGG CCCCTTGGAT 
AAAGAAGACA CTGGAGGGCA GAAGCTTATT CCTACAGGCA GCCTGCCAGC AACTTTACAA 
GGAGCTACAG ATGCTTTAGG ATTGGAATGG CACCTCCCAA GTCCAGATCC CGTCACTGTT 
CCGTATCTCA GTCCTTTGGT GGTGTGGAAG GAACTTGAAA GCTTATTGGA GAATGAAGGC 
GATCATGCAA TAACAGTGGC AGATTTTGTG GACCATCATC CAATTGTTTT TTGGAACCTA 
GTGTGGTATT TTAGACGTCT TGACTTGCCC AGTAACTTAC CTGGATTGAT TCTTTCTTCT 
GAGCATTGTA ACAAATATTC AAAGATTCCC CGCCACTGTA TGTCTGAAGA TAGTAAATAT 
GTTTTAATAC AAATGTTATG GGACAATATG AAATTACATC AGGATCCGGG TCAGCCCTTG 
TACATCCTCT GGAATGCCCA CACCCAAAAG TATCCAATGG TCCATTTATT GCAAAAGGGT 
GATAATTCAT TTAACCAAGA ACTATTGAAA AATATGGTAA AAAGCATTAA AATGAATGAT 
GTCTATGGAC CAATGAGTCA GATTTTAGAG ACACTGAATA AATGTCCTCA TTTTAAAAGA 
CAGAGGAGTT TATATAGAGA AATACTCTTT CTCTCACTTG TGGCACTGGG AAGAGAAAAC 
ATTGACATAG ATGCATTTGA TAAAGAATAC AAGATGGCAT ACGATCGTCT CACCCCTAGT 
CAAGTCAAGA GTACACACAA CTGTGATAGA CCACCAAGTA CGGGGGTGAT GGAATGTCGG 
AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_014733811.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014589297.1 Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X5, mRNA.

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CloneID	OHb10410
Clone ID Related Accession (Same CDS sequence)	XM_014733811.1 , XM_023655127.1
Accession Version	XM_014733811.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867387.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGGTGGCC AGTCTGATCT TGGATATAAC TCATTATCTA AGGATGAAGT TAGAAGAGGA 
CATACATCTA CTGAAGACAT TCAAGAAGAA AAAGATAGGA AAGGGAGTGA TTCTAGTTCT 
TTGTCAGAGA GTGAGAGTGC AAAAGGAAGT GCTGATTGTC TGCCTAAGCT CAGTTTTCAA 
AGTTCTTCCA GTATAGTTCG CCTCAGTGGT GCAAATAACA ACAGTGCTGA TAACACCTCA 
GGGAAAAGCA CAGAAAGCAC ATCTGAGCTG CTTGTAATAT CATCTTTTGA GGACACAAAT 
GAAACAGGAA ACATTCAAAG TAGATGCTTC AGGAAAAGAC ATAAAAGTGA CAATGAACCT 
AATTTGCAGC AGCAAATGTC CTGGGGAAGT AGAAACCGCA ATCTTAGTGG AGGAGTACTG 
ATGGGACTTA TGCTCAATAG GATGAACCAG GAAGCAAACC CTGGTGATAT GGTTGAAAAA 
TTAGGAGCTG ATGCAAAAAT TCTTTCAAAT GTTATCTCAA AAAGCACAAG ACCAAATAGT 
CTTGACATTG GAAAGCCACC TGTAAGATCA AAAAGAGACA GTCTAGAGAA GGAATCTAGT 
GATGATGATA CACCTTTTGA TGGTTCTAAC TGTTTGGCAG ATAAAGTGGA TTCTCCCGTT 
ATTTTTGACT TAGAAGACTT AGACAATGAA ACAGATGGAT CAAAAGTGGG ATATGTTGCT 
ACACAAAATC CCAAGAGGAT TCAGCGTATG AGCAGCAGCT TTTCAGTAAA ACCTTTTGAA 
AAAACTGATG TTGCAACAGG ATTTGATCCC CTCTCTCTTT TGGTTGCTGA GACTGAACAG 
CAGCAAAAAG AGGAGGAGGA AGAGGGGGAA GATGATAGCA AAAGCATTTC AACACCATCT 
GCCAGACGTG ATTTAGCTGA AGAAATTGAG ATGTATATGA ATAACATGAG CAGTCCTTTG 
ACAAGTCGTA CACCAAGCAT TGATTTACAA CGGGCATGTG ATGATAAATT AACCAATAAG 
AAAAGTCCAA CATTGGTCAA GGCATGCAGA AGATCTAGCC TGCCTCCTAA TTCTCCTAGG 
CCAGTGAGAC TTACCAAATC TAAAAGTTAC ACAAAAAGTG AGGACAGGCC AAGGGATAGA 
CTCTGGTCTT CTCCAGCCTT CTCACCCACT TGCCCTTTTA GGGAAGAATC TCAAGATGCA 
TTACCCCACT CATCACCTTC ATTTAATTTG GATACACTAC TAGTACCTAA ACTGGATGTT 
TTAAGGAACA GCATGTTCAC TGCTGGAAAA GGAGTAGCCG AGAAAGCAAG CAAATGGTAT 
TCAAGGTTCA CCATGTATAC TACATCATCT AAGGATCAAA GTTCTGATCG TGCCAGTCTT 
TCTTCAGTGG GAGCCCAGGA TTCTGAATCT GCCTCTTTAA CAGATGAAGA TGTCTGCCAC 
GAGCTGGAAG GAGCCGCTTC CTCCCAAGAG AGCAGTGCCA CTTCAGGGAC CAAGGGAATT 
GATTTCAGCA GAACAAGCCT GGGAAGTTCT GCCTCCTTAG AAGGATCCCT GTCAAAGTTC 
GCCTTACCTG GGAAATCAGA AGTGGCATCT TCCTACAACA CGAGTAATAC AAATATCTTC 
CAGAACTATG CAATGGAGGT TCTCATCTCA AGTTGCTCTC GGTGTAGAAC TTGTGACTGC 
CTTGTCCATG ATGAGGAAAT CATGGCTGGC TGGACAGCAG ATGACTCAAA TCTCAATACC 
ACATGCCCGT TTTGTGGCAA TCTTTTCTTG CCCTTTTTGA ATATTGAAAT AAGAGATTTA 
AGACGACCCG GAAAATACTT TTTGAAGTCA AGCCCATCTG CAGAAAATAT GCATTTTCCG 
TCTTCCTTTT CAAGTCAGAC AAGACAGTCT TGTATCTCAA CATCAGCCTC TGGTCTTGAC 
AAGTCTGTTA TCTCCGTTCC AGGGAATTTT GATCTACATA GCAAATCTAA ACTGCTGGAA 
AATTCATGTG CCCGAAGTAT TCAGATCCCT GCTGATAGAT CAAAAACAGC TGTGTCAAAA 
TGTCCAGTAT TTCCAATGGC CAGGAGCGTC AGTACTTATG GCCCCTTGGA TAAAGAAGAC 
ACTGGAGGGC AGAAGCTTAT TCCTACAGGC AGCCTGCCAG CAACTTTACA AGGAGCTACA 
GATGCTTTAG GATTGGAATG GCACCTCCCA AGTCCAGATC CCGTCACTGT TCCGTATCTC 
AGTCCTTTGG TGGTGTGGAA GGAACTTGAA AGCTTATTGG AGAATGAAGG CGATCATGCA 
ATAACAGTGG CAGATTTTGT GGACCATCAT CCAATTGTTT TTTGGAACCT AGTGTGGTAT 
TTTAGACGTC TTGACTTGCC CAGTAACTTA CCTGGATTGA TTCTTTCTTC TGAGCATTGT 
AACAAATATT CAAAGATTCC CCGCCACTGT ATGTCTGAAG ATAGTAAATA TGTTTTAATA 
CAAATGTTAT GGGACAATAT GAAATTACAT CAGGATCCGG GTCAGCCCTT GTACATCCTC 
TGGAATGCCC ACACCCAAAA GTATCCAATG GTCCATTTAT TGCAAAAGGG TGATAATTCA 
TTTAACCAAG AACTATTGAA AAATATGGTA AAAAGCATTA AAATGAATGA TGTCTATGGA 
CCAATGAGTC AGATTTTAGA GACACTGAAT AAATGTCCTC ATTTTAAAAG ACAGAGGAGT 
TTATATAGAG AAATACTCTT TCTCTCACTT GTGGCACTGG GAAGAGAAAA CATTGACATA 
GATGCATTTG ATAAAGAATA CAAGATGGCA TACGATCGTC TCACCCCTAG TCAAGTCAAG 
AGTACACACA ACTGTGATAG ACCACCAAGT ACGGGGGTGA TGGAATGTCG GAAAACCTTT 
GGAGAACCTT ATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014589297.1
CDS	344..5941
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVVIKSLGEEVPRDYMCIDLTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGPAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_014733811.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN/MADD domain containing 4A (DENND4A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014733811.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGGTGGCC AGTCTGATCT TGGATATAAC TCATTATCTA AGGATGAAGT TAGAAGAGGA 
CATACATCTA CTGAAGACAT TCAAGAAGAA AAAGATAGGA AAGGGAGTGA TTCTAGTTCT 
TTGTCAGAGA GTGAGAGTGC AAAAGGAAGT GCTGATTGTC TGCCTAAGCT CAGTTTTCAA 
AGTTCTTCCA GTATAGTTCG CCTCAGTGGT GCAAATAACA ACAGTGCTGA TAACACCTCA 
GGGAAAAGCA CAGAAAGCAC ATCTGAGCTG CTTGTAATAT CATCTTTTGA GGACACAAAT 
GAAACAGGAA ACATTCAAAG TAGATGCTTC AGGAAAAGAC ATAAAAGTGA CAATGAACCT 
AATTTGCAGC AGCAAATGTC CTGGGGAAGT AGAAACCGCA ATCTTAGTGG AGGAGTACTG 
ATGGGACTTA TGCTCAATAG GATGAACCAG GAAGCAAACC CTGGTGATAT GGTTGAAAAA 
TTAGGAGCTG ATGCAAAAAT TCTTTCAAAT GTTATCTCAA AAAGCACAAG ACCAAATAGT 
CTTGACATTG GAAAGCCACC TGTAAGATCA AAAAGAGACA GTCTAGAGAA GGAATCTAGT 
GATGATGATA CACCTTTTGA TGGTTCTAAC TGTTTGGCAG ATAAAGTGGA TTCTCCCGTT 
ATTTTTGACT TAGAAGACTT AGACAATGAA ACAGATGGAT CAAAAGTGGG ATATGTTGCT 
ACACAAAATC CCAAGAGGAT TCAGCGTATG AGCAGCAGCT TTTCAGTAAA ACCTTTTGAA 
AAAACTGATG TTGCAACAGG ATTTGATCCC CTCTCTCTTT TGGTTGCTGA GACTGAACAG 
CAGCAAAAAG AGGAGGAGGA AGAGGGGGAA GATGATAGCA AAAGCATTTC AACACCATCT 
GCCAGACGTG ATTTAGCTGA AGAAATTGAG ATGTATATGA ATAACATGAG CAGTCCTTTG 
ACAAGTCGTA CACCAAGCAT TGATTTACAA CGGGCATGTG ATGATAAATT AACCAATAAG 
AAAAGTCCAA CATTGGTCAA GGCATGCAGA AGATCTAGCC TGCCTCCTAA TTCTCCTAGG 
CCAGTGAGAC TTACCAAATC TAAAAGTTAC ACAAAAAGTG AGGACAGGCC AAGGGATAGA 
CTCTGGTCTT CTCCAGCCTT CTCACCCACT TGCCCTTTTA GGGAAGAATC TCAAGATGCA 
TTACCCCACT CATCACCTTC ATTTAATTTG GATACACTAC TAGTACCTAA ACTGGATGTT 
TTAAGGAACA GCATGTTCAC TGCTGGAAAA GGAGTAGCCG AGAAAGCAAG CAAATGGTAT 
TCAAGGTTCA CCATGTATAC TACATCATCT AAGGATCAAA GTTCTGATCG TGCCAGTCTT 
TCTTCAGTGG GAGCCCAGGA TTCTGAATCT GCCTCTTTAA CAGATGAAGA TGTCTGCCAC 
GAGCTGGAAG GAGCCGCTTC CTCCCAAGAG AGCAGTGCCA CTTCAGGGAC CAAGGGAATT 
GATTTCAGCA GAACAAGCCT GGGAAGTTCT GCCTCCTTAG AAGGATCCCT GTCAAAGTTC 
GCCTTACCTG GGAAATCAGA AGTGGCATCT TCCTACAACA CGAGTAATAC AAATATCTTC 
CAGAACTATG CAATGGAGGT TCTCATCTCA AGTTGCTCTC GGTGTAGAAC TTGTGACTGC 
CTTGTCCATG ATGAGGAAAT CATGGCTGGC TGGACAGCAG ATGACTCAAA TCTCAATACC 
ACATGCCCGT TTTGTGGCAA TCTTTTCTTG CCCTTTTTGA ATATTGAAAT AAGAGATTTA 
AGACGACCCG GAAAATACTT TTTGAAGTCA AGCCCATCTG CAGAAAATAT GCATTTTCCG 
TCTTCCTTTT CAAGTCAGAC AAGACAGTCT TGTATCTCAA CATCAGCCTC TGGTCTTGAC 
AAGTCTGTTA TCTCCGTTCC AGGGAATTTT GATCTACATA GCAAATCTAA ACTGCTGGAA 
AATTCATGTG CCCGAAGTAT TCAGATCCCT GCTGATAGAT CAAAAACAGC TGTGTCAAAA 
TGTCCAGTAT TTCCAATGGC CAGGAGCGTC AGTACTTATG GCCCCTTGGA TAAAGAAGAC 
ACTGGAGGGC AGAAGCTTAT TCCTACAGGC AGCCTGCCAG CAACTTTACA AGGAGCTACA 
GATGCTTTAG GATTGGAATG GCACCTCCCA AGTCCAGATC CCGTCACTGT TCCGTATCTC 
AGTCCTTTGG TGGTGTGGAA GGAACTTGAA AGCTTATTGG AGAATGAAGG CGATCATGCA 
ATAACAGTGG CAGATTTTGT GGACCATCAT CCAATTGTTT TTTGGAACCT AGTGTGGTAT 
TTTAGACGTC TTGACTTGCC CAGTAACTTA CCTGGATTGA TTCTTTCTTC TGAGCATTGT 
AACAAATATT CAAAGATTCC CCGCCACTGT ATGTCTGAAG ATAGTAAATA TGTTTTAATA 
CAAATGTTAT GGGACAATAT GAAATTACAT CAGGATCCGG GTCAGCCCTT GTACATCCTC 
TGGAATGCCC ACACCCAAAA GTATCCAATG GTCCATTTAT TGCAAAAGGG TGATAATTCA 
TTTAACCAAG AACTATTGAA AAATATGGTA AAAAGCATTA AAATGAATGA TGTCTATGGA 
CCAATGAGTC AGATTTTAGA GACACTGAAT AAATGTCCTC ATTTTAAAAG ACAGAGGAGT 
TTATATAGAG AAATACTCTT TCTCTCACTT GTGGCACTGG GAAGAGAAAA CATTGACATA 
GATGCATTTG ATAAAGAATA CAAGATGGCA TACGATCGTC TCACCCCTAG TCAAGTCAAG 
AGTACACACA ACTGTGATAG ACCACCAAGT ACGGGGGTGA TGGAATGTCG GAAAACCTTT 
GGAGAACCTT ATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_023655127.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023510895.1 Equus caballus DENN domain containing 4A (DENND4A), transcript variant X9, mRNA.

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CloneID	OHb10410
Clone ID Related Accession (Same CDS sequence)	XM_014733811.1 , XM_023655127.1
Accession Version	XM_023655127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	C-myc promoter-binding protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGGTGGCC AGTCTGATCT TGGATATAAC TCATTATCTA AGGATGAAGT TAGAAGAGGA 
CATACATCTA CTGAAGACAT TCAAGAAGAA AAAGATAGGA AAGGGAGTGA TTCTAGTTCT 
TTGTCAGAGA GTGAGAGTGC AAAAGGAAGT GCTGATTGTC TGCCTAAGCT CAGTTTTCAA 
AGTTCTTCCA GTATAGTTCG CCTCAGTGGT GCAAATAACA ACAGTGCTGA TAACACCTCA 
GGGAAAAGCA CAGAAAGCAC ATCTGAGCTG CTTGTAATAT CATCTTTTGA GGACACAAAT 
GAAACAGGAA ACATTCAAAG TAGATGCTTC AGGAAAAGAC ATAAAAGTGA CAATGAACCT 
AATTTGCAGC AGCAAATGTC CTGGGGAAGT AGAAACCGCA ATCTTAGTGG AGGAGTACTG 
ATGGGACTTA TGCTCAATAG GATGAACCAG GAAGCAAACC CTGGTGATAT GGTTGAAAAA 
TTAGGAGCTG ATGCAAAAAT TCTTTCAAAT GTTATCTCAA AAAGCACAAG ACCAAATAGT 
CTTGACATTG GAAAGCCACC TGTAAGATCA AAAAGAGACA GTCTAGAGAA GGAATCTAGT 
GATGATGATA CACCTTTTGA TGGTTCTAAC TGTTTGGCAG ATAAAGTGGA TTCTCCCGTT 
ATTTTTGACT TAGAAGACTT AGACAATGAA ACAGATGGAT CAAAAGTGGG ATATGTTGCT 
ACACAAAATC CCAAGAGGAT TCAGCGTATG AGCAGCAGCT TTTCAGTAAA ACCTTTTGAA 
AAAACTGATG TTGCAACAGG ATTTGATCCC CTCTCTCTTT TGGTTGCTGA GACTGAACAG 
CAGCAAAAAG AGGAGGAGGA AGAGGGGGAA GATGATAGCA AAAGCATTTC AACACCATCT 
GCCAGACGTG ATTTAGCTGA AGAAATTGAG ATGTATATGA ATAACATGAG CAGTCCTTTG 
ACAAGTCGTA CACCAAGCAT TGATTTACAA CGGGCATGTG ATGATAAATT AACCAATAAG 
AAAAGTCCAA CATTGGTCAA GGCATGCAGA AGATCTAGCC TGCCTCCTAA TTCTCCTAGG 
CCAGTGAGAC TTACCAAATC TAAAAGTTAC ACAAAAAGTG AGGACAGGCC AAGGGATAGA 
CTCTGGTCTT CTCCAGCCTT CTCACCCACT TGCCCTTTTA GGGAAGAATC TCAAGATGCA 
TTACCCCACT CATCACCTTC ATTTAATTTG GATACACTAC TAGTACCTAA ACTGGATGTT 
TTAAGGAACA GCATGTTCAC TGCTGGAAAA GGAGTAGCCG AGAAAGCAAG CAAATGGTAT 
TCAAGGTTCA CCATGTATAC TACATCATCT AAGGATCAAA GTTCTGATCG TGCCAGTCTT 
TCTTCAGTGG GAGCCCAGGA TTCTGAATCT GCCTCTTTAA CAGATGAAGA TGTCTGCCAC 
GAGCTGGAAG GAGCCGCTTC CTCCCAAGAG AGCAGTGCCA CTTCAGGGAC CAAGGGAATT 
GATTTCAGCA GAACAAGCCT GGGAAGTTCT GCCTCCTTAG AAGGATCCCT GTCAAAGTTC 
GCCTTACCTG GGAAATCAGA AGTGGCATCT TCCTACAACA CGAGTAATAC AAATATCTTC 
CAGAACTATG CAATGGAGGT TCTCATCTCA AGTTGCTCTC GGTGTAGAAC TTGTGACTGC 
CTTGTCCATG ATGAGGAAAT CATGGCTGGC TGGACAGCAG ATGACTCAAA TCTCAATACC 
ACATGCCCGT TTTGTGGCAA TCTTTTCTTG CCCTTTTTGA ATATTGAAAT AAGAGATTTA 
AGACGACCCG GAAAATACTT TTTGAAGTCA AGCCCATCTG CAGAAAATAT GCATTTTCCG 
TCTTCCTTTT CAAGTCAGAC AAGACAGTCT TGTATCTCAA CATCAGCCTC TGGTCTTGAC 
AAGTCTGTTA TCTCCGTTCC AGGGAATTTT GATCTACATA GCAAATCTAA ACTGCTGGAA 
AATTCATGTG CCCGAAGTAT TCAGATCCCT GCTGATAGAT CAAAAACAGC TGTGTCAAAA 
TGTCCAGTAT TTCCAATGGC CAGGAGCGTC AGTACTTATG GCCCCTTGGA TAAAGAAGAC 
ACTGGAGGGC AGAAGCTTAT TCCTACAGGC AGCCTGCCAG CAACTTTACA AGGAGCTACA 
GATGCTTTAG GATTGGAATG GCACCTCCCA AGTCCAGATC CCGTCACTGT TCCGTATCTC 
AGTCCTTTGG TGGTGTGGAA GGAACTTGAA AGCTTATTGG AGAATGAAGG CGATCATGCA 
ATAACAGTGG CAGATTTTGT GGACCATCAT CCAATTGTTT TTTGGAACCT AGTGTGGTAT 
TTTAGACGTC TTGACTTGCC CAGTAACTTA CCTGGATTGA TTCTTTCTTC TGAGCATTGT 
AACAAATATT CAAAGATTCC CCGCCACTGT ATGTCTGAAG ATAGTAAATA TGTTTTAATA 
CAAATGTTAT GGGACAATAT GAAATTACAT CAGGATCCGG GTCAGCCCTT GTACATCCTC 
TGGAATGCCC ACACCCAAAA GTATCCAATG GTCCATTTAT TGCAAAAGGG TGATAATTCA 
TTTAACCAAG AACTATTGAA AAATATGGTA AAAAGCATTA AAATGAATGA TGTCTATGGA 
CCAATGAGTC AGATTTTAGA GACACTGAAT AAATGTCCTC ATTTTAAAAG ACAGAGGAGT 
TTATATAGAG AAATACTCTT TCTCTCACTT GTGGCACTGG GAAGAGAAAA CATTGACATA 
GATGCATTTG ATAAAGAATA CAAGATGGCA TACGATCGTC TCACCCCTAG TCAAGTCAAG 
AGTACACACA ACTGTGATAG ACCACCAAGT ACGGGGGTGA TGGAATGTCG GAAAACCTTT 
GGAGAACCTT ATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023510895.1
CDS	585..6182
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVVIKSLGEEVPRDYMCIDLTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGPAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIESWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQPGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPREDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDATSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKNGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRSSLFERPEGFLQTRKSKLPSKPSSPGSPSPVFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTGYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKHAHLSQTTLSGGQSDLGYNSLSKDEVRRGHTSTEDIQEEKDRKGSDSSSLSESESAKGSADCLPKLSFQSSSSIVRLSGANNNSADNTSGKSTESTSELLVISSFEDTNETGNIQSRCFRKRHKSDNEPNLQQQMSWGSRNRNLSGGVLMGLMLNRMNQEANPGDMVEKLGADAKILSNVISKSTRPNSLDIGKPPVRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDNETDGSKVGYVATQNPKRIQRMSSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEGEDDSKSISTPSARRDLAEEIEMYMNNMSSPLTSRTPSIDLQRACDDKLTNKKSPTLVKACRRSSLPPNSPRPVRLTKSKSYTKSEDRPRDRLWSSPAFSPTCPFREESQDALPHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRASLSSVGAQDSESASLTDEDVCHELEGAASSQESSATSGTKGIDFSRTSLGSSASLEGSLSKFALPGKSEVASSYNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGKYFLKSSPSAENMHFPSSFSSQTRQSCISTSASGLDKSVISVPGNFDLHSKSKLLENSCARSIQIPADRSKTAVSKCPVFPMARSVSTYGPLDKEDTGGQKLIPTGSLPATLQGATDALGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_023655127.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN domain containing 4A (DENND4A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023655127.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCCAAAGAAC CTATCACAGA TGTTTCAGTT GTTATTAAAT CTCTTGGAGA GGAAGTGCCA 
CGAGATTATA TGTGTATTGA TCTTACCCCA ACTGGATTGT CAGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAG GAAAGATTGA AACAAGGGTG TGAAATTATT 
CAGAGTACTC CCTATGGACG CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACTTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACATTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGACCA GCAGTGTACT TATGTTATAA AAAATCGGTG 
GCAAAGACTA ACACTATATC TTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAGTCTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCTTCATC GGCAGATGGG 
AAGTCTGATA GTTCCAAAAC AATTCACACT AATAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAGTTTCTG ACCTTTTTGT ATCGTTACTC CATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCACCGC ATGATAATCT GATTCTCAGT 
CAGCCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAGT TTTCAACACT GCTTCAGAAT 
TTAGGCCCTG AAAATGCTGT GACACTACTG GTGTTTGCAG TAACAGAACA TAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGTGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTTC ACTGGCCATG CCCGTATGTT CCTCTGTGCC CGCTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATAGTAGGA ATTGATTCAA GATACTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTG GATACCAATA CCATTTCTCA ACCTGGTGAC 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAATCT GATGAACACA 
CTGAATAATT TGCACCAGCA ATTGGCAAAA TTACAGCAGA GACCAAGAGA AGATGGACTA 
ATGGACTTAG CGATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA CATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTACAGATCA 
TACTTAAGAC CAATAACACA GGCCCCATCT GAGACAGCCA CAGATGCAAC CTCTCTTTTT 
GCCCTGCAAG CTTTCTTAAG AAGCCGGGAC CGATCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTTAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ACATGGACAA GAATGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTCTT TGTAACACCT 
CCTGAGATTC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGGAGCAG CTTATTTGAA AGACCTGAAG GGTTTCTGCA GACACGAAAG 
AGCAAATTGC CTTCAAAACC AAGTAGTCCT GGTAGCCCTT CTCCCGTGTT CCGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ATTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTACTGCG TCACTGTTAT GGACTGTGGT TTATTTGTCT CCCAGCTTAT 
GTGAAAGTAT GTCATTCAAA GGTCAGGGCT CTGAAAACGG GATATGATGT GCTTAAGAAA 
ATGCAGTCAA AGAAGATGGA TCCACCCGAT GAGGTGTGCT ACCGCATTCT TATGCAGCTC 
TGTGGACAGT ATGATCAGCC GGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGACC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGTACA 
TGGCCTTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA TGTTGTTTTA 
GGAGTGGCAC AGTTCAAAAG AGCTTTAAAG AAACATGCGC ATTTATCACA AACAACTCTC 
TCAGGTGGCC AGTCTGATCT TGGATATAAC TCATTATCTA AGGATGAAGT TAGAAGAGGA 
CATACATCTA CTGAAGACAT TCAAGAAGAA AAAGATAGGA AAGGGAGTGA TTCTAGTTCT 
TTGTCAGAGA GTGAGAGTGC AAAAGGAAGT GCTGATTGTC TGCCTAAGCT CAGTTTTCAA 
AGTTCTTCCA GTATAGTTCG CCTCAGTGGT GCAAATAACA ACAGTGCTGA TAACACCTCA 
GGGAAAAGCA CAGAAAGCAC ATCTGAGCTG CTTGTAATAT CATCTTTTGA GGACACAAAT 
GAAACAGGAA ACATTCAAAG TAGATGCTTC AGGAAAAGAC ATAAAAGTGA CAATGAACCT 
AATTTGCAGC AGCAAATGTC CTGGGGAAGT AGAAACCGCA ATCTTAGTGG AGGAGTACTG 
ATGGGACTTA TGCTCAATAG GATGAACCAG GAAGCAAACC CTGGTGATAT GGTTGAAAAA 
TTAGGAGCTG ATGCAAAAAT TCTTTCAAAT GTTATCTCAA AAAGCACAAG ACCAAATAGT 
CTTGACATTG GAAAGCCACC TGTAAGATCA AAAAGAGACA GTCTAGAGAA GGAATCTAGT 
GATGATGATA CACCTTTTGA TGGTTCTAAC TGTTTGGCAG ATAAAGTGGA TTCTCCCGTT 
ATTTTTGACT TAGAAGACTT AGACAATGAA ACAGATGGAT CAAAAGTGGG ATATGTTGCT 
ACACAAAATC CCAAGAGGAT TCAGCGTATG AGCAGCAGCT TTTCAGTAAA ACCTTTTGAA 
AAAACTGATG TTGCAACAGG ATTTGATCCC CTCTCTCTTT TGGTTGCTGA GACTGAACAG 
CAGCAAAAAG AGGAGGAGGA AGAGGGGGAA GATGATAGCA AAAGCATTTC AACACCATCT 
GCCAGACGTG ATTTAGCTGA AGAAATTGAG ATGTATATGA ATAACATGAG CAGTCCTTTG 
ACAAGTCGTA CACCAAGCAT TGATTTACAA CGGGCATGTG ATGATAAATT AACCAATAAG 
AAAAGTCCAA CATTGGTCAA GGCATGCAGA AGATCTAGCC TGCCTCCTAA TTCTCCTAGG 
CCAGTGAGAC TTACCAAATC TAAAAGTTAC ACAAAAAGTG AGGACAGGCC AAGGGATAGA 
CTCTGGTCTT CTCCAGCCTT CTCACCCACT TGCCCTTTTA GGGAAGAATC TCAAGATGCA 
TTACCCCACT CATCACCTTC ATTTAATTTG GATACACTAC TAGTACCTAA ACTGGATGTT 
TTAAGGAACA GCATGTTCAC TGCTGGAAAA GGAGTAGCCG AGAAAGCAAG CAAATGGTAT 
TCAAGGTTCA CCATGTATAC TACATCATCT AAGGATCAAA GTTCTGATCG TGCCAGTCTT 
TCTTCAGTGG GAGCCCAGGA TTCTGAATCT GCCTCTTTAA CAGATGAAGA TGTCTGCCAC 
GAGCTGGAAG GAGCCGCTTC CTCCCAAGAG AGCAGTGCCA CTTCAGGGAC CAAGGGAATT 
GATTTCAGCA GAACAAGCCT GGGAAGTTCT GCCTCCTTAG AAGGATCCCT GTCAAAGTTC 
GCCTTACCTG GGAAATCAGA AGTGGCATCT TCCTACAACA CGAGTAATAC AAATATCTTC 
CAGAACTATG CAATGGAGGT TCTCATCTCA AGTTGCTCTC GGTGTAGAAC TTGTGACTGC 
CTTGTCCATG ATGAGGAAAT CATGGCTGGC TGGACAGCAG ATGACTCAAA TCTCAATACC 
ACATGCCCGT TTTGTGGCAA TCTTTTCTTG CCCTTTTTGA ATATTGAAAT AAGAGATTTA 
AGACGACCCG GAAAATACTT TTTGAAGTCA AGCCCATCTG CAGAAAATAT GCATTTTCCG 
TCTTCCTTTT CAAGTCAGAC AAGACAGTCT TGTATCTCAA CATCAGCCTC TGGTCTTGAC 
AAGTCTGTTA TCTCCGTTCC AGGGAATTTT GATCTACATA GCAAATCTAA ACTGCTGGAA 
AATTCATGTG CCCGAAGTAT TCAGATCCCT GCTGATAGAT CAAAAACAGC TGTGTCAAAA 
TGTCCAGTAT TTCCAATGGC CAGGAGCGTC AGTACTTATG GCCCCTTGGA TAAAGAAGAC 
ACTGGAGGGC AGAAGCTTAT TCCTACAGGC AGCCTGCCAG CAACTTTACA AGGAGCTACA 
GATGCTTTAG GATTGGAATG GCACCTCCCA AGTCCAGATC CCGTCACTGT TCCGTATCTC 
AGTCCTTTGG TGGTGTGGAA GGAACTTGAA AGCTTATTGG AGAATGAAGG CGATCATGCA 
ATAACAGTGG CAGATTTTGT GGACCATCAT CCAATTGTTT TTTGGAACCT AGTGTGGTAT 
TTTAGACGTC TTGACTTGCC CAGTAACTTA CCTGGATTGA TTCTTTCTTC TGAGCATTGT 
AACAAATATT CAAAGATTCC CCGCCACTGT ATGTCTGAAG ATAGTAAATA TGTTTTAATA 
CAAATGTTAT GGGACAATAT GAAATTACAT CAGGATCCGG GTCAGCCCTT GTACATCCTC 
TGGAATGCCC ACACCCAAAA GTATCCAATG GTCCATTTAT TGCAAAAGGG TGATAATTCA 
TTTAACCAAG AACTATTGAA AAATATGGTA AAAAGCATTA AAATGAATGA TGTCTATGGA 
CCAATGAGTC AGATTTTAGA GACACTGAAT AAATGTCCTC ATTTTAAAAG ACAGAGGAGT 
TTATATAGAG AAATACTCTT TCTCTCACTT GTGGCACTGG GAAGAGAAAA CATTGACATA 
GATGCATTTG ATAAAGAATA CAAGATGGCA TACGATCGTC TCACCCCTAG TCAAGTCAAG 
AGTACACACA ACTGTGATAG ACCACCAAGT ACGGGGGTGA TGGAATGTCG GAAAACCTTT 
GGAGAACCTT ATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DENND4A
Entrez Gene ID	100053090
Full Name	DENN domain containing 4A
General protein information	Preferred Names DENN domain containing 4A Names C-myc promoter-binding protein
Gene Type	protein-coding
Organism	Equus caballus(horse)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DENND4A cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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