SVIL gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_005617045.3	XP_005617102.1	supervillin isoform X1
XM_005617046.3	XP_005617103.1	supervillin isoform X2
XM_022407049.1	XP_022262757.1	supervillin isoform X4
XM_005617048.3	XP_005617105.1	supervillin isoform X5
XM_005617052.3	XP_005617109.1	supervillin isoform X9
XM_014122029.2	XP_013977504.1	supervillin isoform X9
XM_005617047.2	XP_005617104.1	supervillin isoform X3
XM_005617049.2	XP_005617106.1	supervillin isoform X5
XM_005617051.2	XP_005617108.1	supervillin isoform X7
XM_005617053.2	XP_005617110.1	supervillin isoform X8
XM_005617050.3	XP_005617107.1	supervillin isoform X7
XM_005617054.3	XP_005617111.1	supervillin isoform X12
XM_005617045.2	XP_005617102.1	supervillin isoform X1
XM_005617050.2	XP_005617107.1	supervillin isoform X6
XM_014122029.1	XP_013977504.1	supervillin isoform X8
XM_014122047.1	XP_013977522.1	supervillin isoform X8
XM_022407080.1	XP_022262788.1	supervillin isoform X10
XM_005617048.2	XP_005617105.1	supervillin isoform X4
XM_005617052.2	XP_005617109.1	supervillin isoform X8
XM_005617054.2	XP_005617111.1	supervillin isoform X9
XM_005617049.3	XP_005617106.1	supervillin isoform X6
XM_022407064.1	XP_022262772.1	supervillin isoform X9
XM_022407070.1	XP_022262778.1	supervillin isoform X9
XM_014122047.2	XP_013977522.1	supervillin isoform X9
XM_005617046.2	XP_005617103.1	supervillin isoform X2
XM_005617047.3	XP_005617104.1	supervillin isoform X3
XM_005617051.3	XP_005617108.1	supervillin isoform X8
XM_005617053.3	XP_005617110.1	supervillin isoform X9
XM_022407072.1	XP_022262780.1	supervillin isoform X9
XM_022407082.1	XP_022262790.1	supervillin isoform X11

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe02727	XM_005617054.3 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe286615	XM_022407080.1 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe286616	XM_022407082.1 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe02719	XM_005617045.3 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02720	XM_005617046.3 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02721	XM_005617047.3 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02722	XM_005617048.3 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02723	XM_005617049.3 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02724	XM_005617050.3 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02725	XM_005617051.3 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02726	XM_022407072.1 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02726	XM_005617053.3 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02726	XM_022407070.1 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02726	XM_005617052.3 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02726	XM_014122029.2 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02726	XM_014122047.2 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02726	XM_022407064.1 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe286614	XM_022407049.1 Latest version!	Canis lupus familiaris supervillin (SVIL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCe02719	XM_005617045.2	Canis lupus familiaris supervillin (SVIL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02724	XM_005617050.2	Canis lupus familiaris supervillin (SVIL), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02726	XM_014122029.1	Canis lupus familiaris supervillin (SVIL), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02722	XM_005617048.2	Canis lupus familiaris supervillin (SVIL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02726	XM_005617052.2	Canis lupus familiaris supervillin (SVIL), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02727	XM_005617054.2	Canis lupus familiaris supervillin (SVIL), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe02723	XM_005617049.2	Canis lupus familiaris supervillin (SVIL), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02726	XM_014122047.1	Canis lupus familiaris supervillin (SVIL), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02720	XM_005617046.2	Canis lupus familiaris supervillin (SVIL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02721	XM_005617047.2	Canis lupus familiaris supervillin (SVIL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02725	XM_005617051.2	Canis lupus familiaris supervillin (SVIL), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe02726	XM_005617053.2	Canis lupus familiaris supervillin (SVIL), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

SVIL ( XM_005617054.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617111.1 Canis lupus familiaris supervillin (SVIL), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OCe02727
Clone ID Related Accession (Same CDS sequence)	XM_005617054.3 , XM_005617054.2
Accession Version	XM_005617054.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617054.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGGTCT 
GCTTCGCATT CAGAAATGCC AACCACTGAG GATGAAGAAA AGGTAGATGA ACGAGCCAAG 
CTGAGTGTCG CTGCCAAAAG GCTGCTTTTC AGGGAAATGG AAAAATCATT TGATGAGAAA 
AATGTTCCAA AGCGACGCTC CAGAAACGCG GCTGTGGAGC AAAGGCTGCG TCGGCTACAG 
GATAGATCCC ACACCCAGCC CGTTACCACC GAGGAAGTGG CCATTGCAGC CACGTTGCAG 
GCATCTGCTC ACCACAAAGC CTTAGCCAGA GACCAGGCAA ATGAGGGCAA AGATGCTGCT 
GAACAAGGTG AACCTGACTC CTCCACTCTA AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC 
AAATTATCCC AGCCAGTCTC AAAAGCTATT TCTACCCGAA ACAGAATAGA CATGAGACAG 
AGGAGAATGA ATGCTCGCTA TCAGACTCAG CCAGTCACGC TTGGAGAGGT GGAACAGGTG 
CAAAGTGGAA AGCTCATTCC TTTCTCACCT ACCGTTAACA CATCTGTGTC CACCATGGCA 
TCTACAGTCA CTCCAATGTA TGCAGGGGAT CTTCGGACAA AGTTGCCTGT GGATGACAGA 
ACAAGTGCCA CTGACCATAA ATTTCCTTCT TTGATGGAAA ATTCAGATTC TCCGGTTAGA 
AGCATTCTGA AGTCCCAAGT CTGGCAGCCT TTGACTGAGG GCAGTGGAAA CAGAGGAATG 
TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA 
AAGTACGGGT CCTTTGAGGA AGTGGAGCCA CCCTGTCCTA CCCTGAACAG AGTCTGGGAA 
GGAGACAGCC ATAAGGAAAC CAAATATGCT GTTCCAAGAA AGGGTAGCCT GGAACTAGCT 
CACCCTCCCG TTGCCCACCT TGGCGATGAA CTGAAGGAGT TCTCCGTGGG TAAAAGCATT 
GCACAGGGGA GCCCAGACTG GACAGATAGG CAGCCCTTTG AAGAGAAGGT TGACCTGGAA 
AATGTCACGA GAAGGAGGTT TTCGCTAAGA GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG 
ACGGGAACAG CAGCCGGGAA AACCGTCACC CAGACTGCAG CCCCCATGTC CTGGAAGCAG 
CATGATTCTG TAGAACCAGT ACAGGAGAAG TACCCCAGGA ATCCATGTGC CATGTTTGCT 
GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG GGCATTCTGG ATTCAGCCAG CAAAACCATG 
TCAATTAAAG AAAGATTAGC GCTGTTGAAG AAGAGTGGTG AAGAAGATTG GAAGAACAGA 
CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG TCTGTCTCCA GCAGCCTGCA CATACAAGAA 
GCGGAACAGT CCCTCAAGAA GAAGCGGGTC ACAGAAAGTC GAGAGAGCCA GATGACTATC 
GAAGAGAGGA AGCATCTCAT CACTGTGAGA GAAGAAGCTT GGAAGACAAA AGGCAAAGGA 
GCGGCCAACG ACTCCACCCA GTTTACGGTG GCTGGCAGAA TGGTGAAGAA AGGTTTGGCA 
TCACCCACCG CCATAACTCC AGTAGCGTCT CCTATTTGCA GTAAAACGAG GGGCACCACA 
CCTGTTTCTA AACCTTTGGA AGATATTGAA GCCAGACCAG ACATGCAGTT GGAATCGGAC 
CTGAAGTTGG ACAGGCTGGA AACCTTTCTA AGAAGGCTGA ATAACAAAGT TGGTGGGATG 
CAGGAGACTG TACTCACTGT CACCGGTAAA TCTGTTAAAG AGGTGATGAA GTTGGATGAT 
GACGAGACCT TTGCCAGATT TTACCGCAGC ATGGATTATA CTGTGCCACG AAGCCCCGTG 
GAGCTAGACG AAGATTTCGA TGTCATTTTC GATCCTTATG CCCCTAAGTT GACCTCCTCT 
GTGGCCGAGC ACAAGCGCGC AGTTAGGCCC AAGCGCCGGG TTCAGGCTTC CAGAAACCCC 
TTGAAAATGC TGGCAGCCAG AGAAGATCTC CTTCAGGAGT ACACGGAGCA GAGACTAAAC 
ATTGCCTTCA TGGAGTCAAA GCGGATGAAA GTAGAAAAGA TGTCCTCCAA CTCCAACTTC 
TCAGAAGTCA CTCTGGCAGG CTTAGCCAGT AAAGAAAACT TCAGCAACAT CAGCCTGCGG 
AGCGTCAACC TGACGGAACA GAATTCCAAC AATAGCGCGG TACCCTATAA GAAGCTGATG 
TTGTTACAGA TTAAAGGAAG AAGACACGTG CAGACAAGAC TGGTCGAACC TCGAGCTTCA 
TCCCTCAACA GTGGGGACTG CTTCCTCCTG CTCTCCCCTC ACTACTGCTT CGTGTGGGTA 
GGAGAGTTTG CAAATGTCAT AGAAAAGGCA AAGGCCTCGG AACTTGCAAG TTTAATTCAG 
ACAAAGAGGG AACTTGGTTG TAGAGCTACA TATGTCCAAA CTATCGAAGA AGGAATTAAT 
ACACACACTC ATGCGGCCAA AGACTTCTGG AAGCTTCTGG GTGGCCAAAC CAGTTACCAG 
TCTGCCGGAG ACCCAAAAGA AGATGAACTT TATGAAACAG CCATAATTGA AACAAACTGC 
ATTTACCGTC TGATGGATGA CAAACTGGTC CCTGAGGACG ACTACTGGGG AAAGATTCCC 
AAGTGCTCCC TTCTGCAGTC AAAAGAGGTA CTGGTGTTTG ATTTTGGTAG TGAAGTGTAC 
GTGTGGCATG GAAAAGAAGT CACATTAGCG CAACGGAAAA TAGCTTTTCA ACTGGCAAAG 
CACTTATGGA ACGGAACCTT TGACTACGAG AACTGTGACA TCAACCCGTT GGATCCTGGA 
GAATGCAATC CGCTTATTCC CAGAAAAGGA CAAGGGCGAC CGGATTGGGC AATTTTCGGG 
AGACTTACAG AACACAATGA GACGATTTTG TTCAAAGAGA AATTTCTGGA TTGGACGGAA 
CTGAAGAGGC CTAATGAGAA GAGTGCCGGC GAACTCACCC AGCAAAAGGA AGATCCCAGG 
GCAGAAGCCA AGGCATATGA CGTGACACGG ATGGTGCCGG CACCCCAGGC CACAACCGGC 
ACTGTGCTGG ATGGGGTGAA TGTTGGTCGT GGCTACGGCT TGGTGGATGG GGATGACAGG 
AGGCAGTTTG AGATCGCCAG TGTGTCTGTG GATGTGTGGC ACATCCTAGA ATTTGACTAC 
AGCAGGCTAC CCAAACAGAG CATCGGGCAG TTCCACGAGG GAGACGCCTA TGTGGTCAAG 
TGGAAGTACA TGGTGAGCAC CGCAGTGGGA AGTCGACAAA AGGGAGAGCA CCCAGTACGG 
GTAGCTGGCA AGGAGAAGTG TGTCTACTTC TTCTGGCAAG GCCGGCAGTC GACCGTGAGT 
GAGAAGGGCA CATCAGCTCT GATGACAGTG GAGCTGGATG AGGAGAGGGG TGCCCAGGTC 
CAGGTTCTGC AGGGAAAGGA GCCCCCCTGC TTTCTGCAGT GTTTCCAGGG GGGAATGGTG 
GTACACTCTG GGAGACGGGA AGAGGAGGAA GAAAATGCAC AAAGTGAGTG GAGGCTGTAC 
TGTGTCCGAG GAGAAGTGCC CATGGAAGGA AATTTGCTGG AAGTGGCCTG TCACTGTAGT 
AGCCTGAGGT CCAGGACTTC CATGGTTGTC CTCAACATAA ACAAAGCCCT CATTTACTTA 
TGGCACGGAT GCAAAGCCCA AGCCCACACG AAGGAGGTTG GAAGGACTGC AGCAAATAAA 
ATCAAAGAAC AATGTCCCCT GGAAGCAGGA CTGCATAGTA GCAGCAAAGT GACAATACAC 
GAGTGTGACG AAGGATCCGA GCCGCTGGGA TTCTGGGATG CTTTAGGAAG GAGAGATAGA 
AAAGCGTACG ACTGTATGCT TCAAGATCCT GGAAATTTTA ACTTCACACC TCGCCTATTC 
ATCCTCAGCA GTTCCTCCGG TGACTTCTTA GCCACAGAGT TCATATATCC CGCCCGAGAC 
CCCTCTGTGG TCAACTCCAT GCCTTTCCTG CAGGAAGATC TTTACAGCGC GCCACAGCCA 
GCACTTTTCC TTGTTGACAA TCACCACGAG GTATACCTCT GGCAAGGCTG GTGGCCCATA 
GAGAACAAGA TCACTGGATC GGCCCGCATT CGCTGGGCCT CCGACCGGAA GAGCGCCATG 
GAAACCGTGC TCCAGTACTG CAAAGGAAAA AATATCAAGA GGCCGCCCCC CAAGTCTTAC 
CTTATCCATG CTGGTCTGGA GCCCTTGACA TTCACCAACA TGTTCCCCAG CTGGGAGCAC 
AGAGAGGACA TTGCTGAAAT CACAGAAATG GATACCGAAG TTTCAAATCA GATCACCCTT 
GTGGAAGATG TCTTAGCCAA GCTCTGTAAA ACCATCTATC CCCTGGCTGA TCTGCTAGCC 
AGGCCGCTAC CCGAGGGAGT TGATCCTCTG AAGCTTGAGA TTTATCTCAC CGATGAAGAC 
TTTGAGTTTG CACTAGACAT GACAAGAGAC GAATACAATG CTCTGCCCGC CTGGAAGCAG 
GTGAACCTGA AGAAAGCAAA GGGTCTGTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617111.1
CDS	57..5669
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARARSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617054.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617054.3

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGGTCT 
GCTTCGCATT CAGAAATGCC AACCACTGAG GATGAAGAAA AGGTAGATGA ACGAGCCAAG 
CTGAGTGTCG CTGCCAAAAG GCTGCTTTTC AGGGAAATGG AAAAATCATT TGATGAGAAA 
AATGTTCCAA AGCGACGCTC CAGAAACGCG GCTGTGGAGC AAAGGCTGCG TCGGCTACAG 
GATAGATCCC ACACCCAGCC CGTTACCACC GAGGAAGTGG CCATTGCAGC CACGTTGCAG 
GCATCTGCTC ACCACAAAGC CTTAGCCAGA GACCAGGCAA ATGAGGGCAA AGATGCTGCT 
GAACAAGGTG AACCTGACTC CTCCACTCTA AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC 
AAATTATCCC AGCCAGTCTC AAAAGCTATT TCTACCCGAA ACAGAATAGA CATGAGACAG 
AGGAGAATGA ATGCTCGCTA TCAGACTCAG CCAGTCACGC TTGGAGAGGT GGAACAGGTG 
CAAAGTGGAA AGCTCATTCC TTTCTCACCT ACCGTTAACA CATCTGTGTC CACCATGGCA 
TCTACAGTCA CTCCAATGTA TGCAGGGGAT CTTCGGACAA AGTTGCCTGT GGATGACAGA 
ACAAGTGCCA CTGACCATAA ATTTCCTTCT TTGATGGAAA ATTCAGATTC TCCGGTTAGA 
AGCATTCTGA AGTCCCAAGT CTGGCAGCCT TTGACTGAGG GCAGTGGAAA CAGAGGAATG 
TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA 
AAGTACGGGT CCTTTGAGGA AGTGGAGCCA CCCTGTCCTA CCCTGAACAG AGTCTGGGAA 
GGAGACAGCC ATAAGGAAAC CAAATATGCT GTTCCAAGAA AGGGTAGCCT GGAACTAGCT 
CACCCTCCCG TTGCCCACCT TGGCGATGAA CTGAAGGAGT TCTCCGTGGG TAAAAGCATT 
GCACAGGGGA GCCCAGACTG GACAGATAGG CAGCCCTTTG AAGAGAAGGT TGACCTGGAA 
AATGTCACGA GAAGGAGGTT TTCGCTAAGA GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG 
ACGGGAACAG CAGCCGGGAA AACCGTCACC CAGACTGCAG CCCCCATGTC CTGGAAGCAG 
CATGATTCTG TAGAACCAGT ACAGGAGAAG TACCCCAGGA ATCCATGTGC CATGTTTGCT 
GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG GGCATTCTGG ATTCAGCCAG CAAAACCATG 
TCAATTAAAG AAAGATTAGC GCTGTTGAAG AAGAGTGGTG AAGAAGATTG GAAGAACAGA 
CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG TCTGTCTCCA GCAGCCTGCA CATACAAGAA 
GCGGAACAGT CCCTCAAGAA GAAGCGGGTC ACAGAAAGTC GAGAGAGCCA GATGACTATC 
GAAGAGAGGA AGCATCTCAT CACTGTGAGA GAAGAAGCTT GGAAGACAAA AGGCAAAGGA 
GCGGCCAACG ACTCCACCCA GTTTACGGTG GCTGGCAGAA TGGTGAAGAA AGGTTTGGCA 
TCACCCACCG CCATAACTCC AGTAGCGTCT CCTATTTGCA GTAAAACGAG GGGCACCACA 
CCTGTTTCTA AACCTTTGGA AGATATTGAA GCCAGACCAG ACATGCAGTT GGAATCGGAC 
CTGAAGTTGG ACAGGCTGGA AACCTTTCTA AGAAGGCTGA ATAACAAAGT TGGTGGGATG 
CAGGAGACTG TACTCACTGT CACCGGTAAA TCTGTTAAAG AGGTGATGAA GTTGGATGAT 
GACGAGACCT TTGCCAGATT TTACCGCAGC ATGGATTATA CTGTGCCACG AAGCCCCGTG 
GAGCTAGACG AAGATTTCGA TGTCATTTTC GATCCTTATG CCCCTAAGTT GACCTCCTCT 
GTGGCCGAGC ACAAGCGCGC AGTTAGGCCC AAGCGCCGGG TTCAGGCTTC CAGAAACCCC 
TTGAAAATGC TGGCAGCCAG AGAAGATCTC CTTCAGGAGT ACACGGAGCA GAGACTAAAC 
ATTGCCTTCA TGGAGTCAAA GCGGATGAAA GTAGAAAAGA TGTCCTCCAA CTCCAACTTC 
TCAGAAGTCA CTCTGGCAGG CTTAGCCAGT AAAGAAAACT TCAGCAACAT CAGCCTGCGG 
AGCGTCAACC TGACGGAACA GAATTCCAAC AATAGCGCGG TACCCTATAA GAAGCTGATG 
TTGTTACAGA TTAAAGGAAG AAGACACGTG CAGACAAGAC TGGTCGAACC TCGAGCTTCA 
TCCCTCAACA GTGGGGACTG CTTCCTCCTG CTCTCCCCTC ACTACTGCTT CGTGTGGGTA 
GGAGAGTTTG CAAATGTCAT AGAAAAGGCA AAGGCCTCGG AACTTGCAAG TTTAATTCAG 
ACAAAGAGGG AACTTGGTTG TAGAGCTACA TATGTCCAAA CTATCGAAGA AGGAATTAAT 
ACACACACTC ATGCGGCCAA AGACTTCTGG AAGCTTCTGG GTGGCCAAAC CAGTTACCAG 
TCTGCCGGAG ACCCAAAAGA AGATGAACTT TATGAAACAG CCATAATTGA AACAAACTGC 
ATTTACCGTC TGATGGATGA CAAACTGGTC CCTGAGGACG ACTACTGGGG AAAGATTCCC 
AAGTGCTCCC TTCTGCAGTC AAAAGAGGTA CTGGTGTTTG ATTTTGGTAG TGAAGTGTAC 
GTGTGGCATG GAAAAGAAGT CACATTAGCG CAACGGAAAA TAGCTTTTCA ACTGGCAAAG 
CACTTATGGA ACGGAACCTT TGACTACGAG AACTGTGACA TCAACCCGTT GGATCCTGGA 
GAATGCAATC CGCTTATTCC CAGAAAAGGA CAAGGGCGAC CGGATTGGGC AATTTTCGGG 
AGACTTACAG AACACAATGA GACGATTTTG TTCAAAGAGA AATTTCTGGA TTGGACGGAA 
CTGAAGAGGC CTAATGAGAA GAGTGCCGGC GAACTCACCC AGCAAAAGGA AGATCCCAGG 
GCAGAAGCCA AGGCATATGA CGTGACACGG ATGGTGCCGG CACCCCAGGC CACAACCGGC 
ACTGTGCTGG ATGGGGTGAA TGTTGGTCGT GGCTACGGCT TGGTGGATGG GGATGACAGG 
AGGCAGTTTG AGATCGCCAG TGTGTCTGTG GATGTGTGGC ACATCCTAGA ATTTGACTAC 
AGCAGGCTAC CCAAACAGAG CATCGGGCAG TTCCACGAGG GAGACGCCTA TGTGGTCAAG 
TGGAAGTACA TGGTGAGCAC CGCAGTGGGA AGTCGACAAA AGGGAGAGCA CCCAGTACGG 
GTAGCTGGCA AGGAGAAGTG TGTCTACTTC TTCTGGCAAG GCCGGCAGTC GACCGTGAGT 
GAGAAGGGCA CATCAGCTCT GATGACAGTG GAGCTGGATG AGGAGAGGGG TGCCCAGGTC 
CAGGTTCTGC AGGGAAAGGA GCCCCCCTGC TTTCTGCAGT GTTTCCAGGG GGGAATGGTG 
GTACACTCTG GGAGACGGGA AGAGGAGGAA GAAAATGCAC AAAGTGAGTG GAGGCTGTAC 
TGTGTCCGAG GAGAAGTGCC CATGGAAGGA AATTTGCTGG AAGTGGCCTG TCACTGTAGT 
AGCCTGAGGT CCAGGACTTC CATGGTTGTC CTCAACATAA ACAAAGCCCT CATTTACTTA 
TGGCACGGAT GCAAAGCCCA AGCCCACACG AAGGAGGTTG GAAGGACTGC AGCAAATAAA 
ATCAAAGAAC AATGTCCCCT GGAAGCAGGA CTGCATAGTA GCAGCAAAGT GACAATACAC 
GAGTGTGACG AAGGATCCGA GCCGCTGGGA TTCTGGGATG CTTTAGGAAG GAGAGATAGA 
AAAGCGTACG ACTGTATGCT TCAAGATCCT GGAAATTTTA ACTTCACACC TCGCCTATTC 
ATCCTCAGCA GTTCCTCCGG TGACTTCTTA GCCACAGAGT TCATATATCC CGCCCGAGAC 
CCCTCTGTGG TCAACTCCAT GCCTTTCCTG CAGGAAGATC TTTACAGCGC GCCACAGCCA 
GCACTTTTCC TTGTTGACAA TCACCACGAG GTATACCTCT GGCAAGGCTG GTGGCCCATA 
GAGAACAAGA TCACTGGATC GGCCCGCATT CGCTGGGCCT CCGACCGGAA GAGCGCCATG 
GAAACCGTGC TCCAGTACTG CAAAGGAAAA AATATCAAGA GGCCGCCCCC CAAGTCTTAC 
CTTATCCATG CTGGTCTGGA GCCCTTGACA TTCACCAACA TGTTCCCCAG CTGGGAGCAC 
AGAGAGGACA TTGCTGAAAT CACAGAAATG GATACCGAAG TTTCAAATCA GATCACCCTT 
GTGGAAGATG TCTTAGCCAA GCTCTGTAAA ACCATCTATC CCCTGGCTGA TCTGCTAGCC 
AGGCCGCTAC CCGAGGGAGT TGATCCTCTG AAGCTTGAGA TTTATCTCAC CGATGAAGAC 
TTTGAGTTTG CACTAGACAT GACAAGAGAC GAATACAATG CTCTGCCCGC CTGGAAGCAG 
GTGAACCTGA AGAAAGCAAA GGGTCTGTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617054.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617111.1 Canis lupus familiaris supervillin (SVIL), transcript variant X12, mRNA.

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CloneID	OCe02727
Clone ID Related Accession (Same CDS sequence)	XM_005617054.3 , XM_005617054.2
Accession Version	XM_005617054.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	supervillin isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726048.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617054.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGGTCT 
GCTTCGCATT CAGAAATGCC AACCACTGAG GATGAAGAAA AGGTAGATGA ACGAGCCAAG 
CTGAGTGTCG CTGCCAAAAG GCTGCTTTTC AGGGAAATGG AAAAATCATT TGATGAGAAA 
AATGTTCCAA AGCGACGCTC CAGAAACGCG GCTGTGGAGC AAAGGCTGCG TCGGCTACAG 
GATAGATCCC ACACCCAGCC CGTTACCACC GAGGAAGTGG CCATTGCAGC CACGTTGCAG 
GCATCTGCTC ACCACAAAGC CTTAGCCAGA GACCAGGCAA ATGAGGGCAA AGATGCTGCT 
GAACAAGGTG AACCTGACTC CTCCACTCTA AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC 
AAATTATCCC AGCCAGTCTC AAAAGCTATT TCTACCCGAA ACAGAATAGA CATGAGACAG 
AGGAGAATGA ATGCTCGCTA TCAGACTCAG CCAGTCACGC TTGGAGAGGT GGAACAGGTG 
CAAAGTGGAA AGCTCATTCC TTTCTCACCT ACCGTTAACA CATCTGTGTC CACCATGGCA 
TCTACAGTCA CTCCAATGTA TGCAGGGGAT CTTCGGACAA AGTTGCCTGT GGATGACAGA 
ACAAGTGCCA CTGACCATAA ATTTCCTTCT TTGATGGAAA ATTCAGATTC TCCGGTTAGA 
AGCATTCTGA AGTCCCAAGT CTGGCAGCCT TTGACTGAGG GCAGTGGAAA CAGAGGAATG 
TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA 
AAGTACGGGT CCTTTGAGGA AGTGGAGCCA CCCTGTCCTA CCCTGAACAG AGTCTGGGAA 
GGAGACAGCC ATAAGGAAAC CAAATATGCT GTTCCAAGAA AGGGTAGCCT GGAACTAGCT 
CACCCTCCCG TTGCCCACCT TGGCGATGAA CTGAAGGAGT TCTCCGTGGG TAAAAGCATT 
GCACAGGGGA GCCCAGACTG GACAGATAGG CAGCCCTTTG AAGAGAAGGT TGACCTGGAA 
AATGTCACGA GAAGGAGGTT TTCGCTAAGA GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG 
ACGGGAACAG CAGCCGGGAA AACCGTCACC CAGACTGCAG CCCCCATGTC CTGGAAGCAG 
CATGATTCTG TAGAACCAGT ACAGGAGAAG TACCCCAGGA ATCCATGTGC CATGTTTGCT 
GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG GGCATTCTGG ATTCAGCCAG CAAAACCATG 
TCAATTAAAG AAAGATTAGC GCTGTTGAAG AAGAGTGGTG AAGAAGATTG GAAGAACAGA 
CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG TCTGTCTCCA GCAGCCTGCA CATACAAGAA 
GCGGAACAGT CCCTCAAGAA GAAGCGGGTC ACAGAAAGTC GAGAGAGCCA GATGACTATC 
GAAGAGAGGA AGCATCTCAT CACTGTGAGA GAAGAAGCTT GGAAGACAAA AGGCAAAGGA 
GCGGCCAACG ACTCCACCCA GTTTACGGTG GCTGGCAGAA TGGTGAAGAA AGGTTTGGCA 
TCACCCACCG CCATAACTCC AGTAGCGTCT CCTATTTGCA GTAAAACGAG GGGCACCACA 
CCTGTTTCTA AACCTTTGGA AGATATTGAA GCCAGACCAG ACATGCAGTT GGAATCGGAC 
CTGAAGTTGG ACAGGCTGGA AACCTTTCTA AGAAGGCTGA ATAACAAAGT TGGTGGGATG 
CAGGAGACTG TACTCACTGT CACCGGTAAA TCTGTTAAAG AGGTGATGAA GTTGGATGAT 
GACGAGACCT TTGCCAGATT TTACCGCAGC ATGGATTATA CTGTGCCACG AAGCCCCGTG 
GAGCTAGACG AAGATTTCGA TGTCATTTTC GATCCTTATG CCCCTAAGTT GACCTCCTCT 
GTGGCCGAGC ACAAGCGCGC AGTTAGGCCC AAGCGCCGGG TTCAGGCTTC CAGAAACCCC 
TTGAAAATGC TGGCAGCCAG AGAAGATCTC CTTCAGGAGT ACACGGAGCA GAGACTAAAC 
ATTGCCTTCA TGGAGTCAAA GCGGATGAAA GTAGAAAAGA TGTCCTCCAA CTCCAACTTC 
TCAGAAGTCA CTCTGGCAGG CTTAGCCAGT AAAGAAAACT TCAGCAACAT CAGCCTGCGG 
AGCGTCAACC TGACGGAACA GAATTCCAAC AATAGCGCGG TACCCTATAA GAAGCTGATG 
TTGTTACAGA TTAAAGGAAG AAGACACGTG CAGACAAGAC TGGTCGAACC TCGAGCTTCA 
TCCCTCAACA GTGGGGACTG CTTCCTCCTG CTCTCCCCTC ACTACTGCTT CGTGTGGGTA 
GGAGAGTTTG CAAATGTCAT AGAAAAGGCA AAGGCCTCGG AACTTGCAAG TTTAATTCAG 
ACAAAGAGGG AACTTGGTTG TAGAGCTACA TATGTCCAAA CTATCGAAGA AGGAATTAAT 
ACACACACTC ATGCGGCCAA AGACTTCTGG AAGCTTCTGG GTGGCCAAAC CAGTTACCAG 
TCTGCCGGAG ACCCAAAAGA AGATGAACTT TATGAAACAG CCATAATTGA AACAAACTGC 
ATTTACCGTC TGATGGATGA CAAACTGGTC CCTGAGGACG ACTACTGGGG AAAGATTCCC 
AAGTGCTCCC TTCTGCAGTC AAAAGAGGTA CTGGTGTTTG ATTTTGGTAG TGAAGTGTAC 
GTGTGGCATG GAAAAGAAGT CACATTAGCG CAACGGAAAA TAGCTTTTCA ACTGGCAAAG 
CACTTATGGA ACGGAACCTT TGACTACGAG AACTGTGACA TCAACCCGTT GGATCCTGGA 
GAATGCAATC CGCTTATTCC CAGAAAAGGA CAAGGGCGAC CGGATTGGGC AATTTTCGGG 
AGACTTACAG AACACAATGA GACGATTTTG TTCAAAGAGA AATTTCTGGA TTGGACGGAA 
CTGAAGAGGC CTAATGAGAA GAGTGCCGGC GAACTCACCC AGCAAAAGGA AGATCCCAGG 
GCAGAAGCCA AGGCATATGA CGTGACACGG ATGGTGCCGG CACCCCAGGC CACAACCGGC 
ACTGTGCTGG ATGGGGTGAA TGTTGGTCGT GGCTACGGCT TGGTGGATGG GGATGACAGG 
AGGCAGTTTG AGATCGCCAG TGTGTCTGTG GATGTGTGGC ACATCCTAGA ATTTGACTAC 
AGCAGGCTAC CCAAACAGAG CATCGGGCAG TTCCACGAGG GAGACGCCTA TGTGGTCAAG 
TGGAAGTACA TGGTGAGCAC CGCAGTGGGA AGTCGACAAA AGGGAGAGCA CCCAGTACGG 
GTAGCTGGCA AGGAGAAGTG TGTCTACTTC TTCTGGCAAG GCCGGCAGTC GACCGTGAGT 
GAGAAGGGCA CATCAGCTCT GATGACAGTG GAGCTGGATG AGGAGAGGGG TGCCCAGGTC 
CAGGTTCTGC AGGGAAAGGA GCCCCCCTGC TTTCTGCAGT GTTTCCAGGG GGGAATGGTG 
GTACACTCTG GGAGACGGGA AGAGGAGGAA GAAAATGCAC AAAGTGAGTG GAGGCTGTAC 
TGTGTCCGAG GAGAAGTGCC CATGGAAGGA AATTTGCTGG AAGTGGCCTG TCACTGTAGT 
AGCCTGAGGT CCAGGACTTC CATGGTTGTC CTCAACATAA ACAAAGCCCT CATTTACTTA 
TGGCACGGAT GCAAAGCCCA AGCCCACACG AAGGAGGTTG GAAGGACTGC AGCAAATAAA 
ATCAAAGAAC AATGTCCCCT GGAAGCAGGA CTGCATAGTA GCAGCAAAGT GACAATACAC 
GAGTGTGACG AAGGATCCGA GCCGCTGGGA TTCTGGGATG CTTTAGGAAG GAGAGATAGA 
AAAGCGTACG ACTGTATGCT TCAAGATCCT GGAAATTTTA ACTTCACACC TCGCCTATTC 
ATCCTCAGCA GTTCCTCCGG TGACTTCTTA GCCACAGAGT TCATATATCC CGCCCGAGAC 
CCCTCTGTGG TCAACTCCAT GCCTTTCCTG CAGGAAGATC TTTACAGCGC GCCACAGCCA 
GCACTTTTCC TTGTTGACAA TCACCACGAG GTATACCTCT GGCAAGGCTG GTGGCCCATA 
GAGAACAAGA TCACTGGATC GGCCCGCATT CGCTGGGCCT CCGACCGGAA GAGCGCCATG 
GAAACCGTGC TCCAGTACTG CAAAGGAAAA AATATCAAGA GGCCGCCCCC CAAGTCTTAC 
CTTATCCATG CTGGTCTGGA GCCCTTGACA TTCACCAACA TGTTCCCCAG CTGGGAGCAC 
AGAGAGGACA TTGCTGAAAT CACAGAAATG GATACCGAAG TTTCAAATCA GATCACCCTT 
GTGGAAGATG TCTTAGCCAA GCTCTGTAAA ACCATCTATC CCCTGGCTGA TCTGCTAGCC 
AGGCCGCTAC CCGAGGGAGT TGATCCTCTG AAGCTTGAGA TTTATCTCAC CGATGAAGAC 
TTTGAGTTTG CACTAGACAT GACAAGAGAC GAATACAATG CTCTGCCCGC CTGGAAGCAG 
GTGAACCTGA AGAAAGCAAA GGGTCTGTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617111.1
CDS	40..5652
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARARSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617054.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617054.2

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGGTCT 
GCTTCGCATT CAGAAATGCC AACCACTGAG GATGAAGAAA AGGTAGATGA ACGAGCCAAG 
CTGAGTGTCG CTGCCAAAAG GCTGCTTTTC AGGGAAATGG AAAAATCATT TGATGAGAAA 
AATGTTCCAA AGCGACGCTC CAGAAACGCG GCTGTGGAGC AAAGGCTGCG TCGGCTACAG 
GATAGATCCC ACACCCAGCC CGTTACCACC GAGGAAGTGG CCATTGCAGC CACGTTGCAG 
GCATCTGCTC ACCACAAAGC CTTAGCCAGA GACCAGGCAA ATGAGGGCAA AGATGCTGCT 
GAACAAGGTG AACCTGACTC CTCCACTCTA AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC 
AAATTATCCC AGCCAGTCTC AAAAGCTATT TCTACCCGAA ACAGAATAGA CATGAGACAG 
AGGAGAATGA ATGCTCGCTA TCAGACTCAG CCAGTCACGC TTGGAGAGGT GGAACAGGTG 
CAAAGTGGAA AGCTCATTCC TTTCTCACCT ACCGTTAACA CATCTGTGTC CACCATGGCA 
TCTACAGTCA CTCCAATGTA TGCAGGGGAT CTTCGGACAA AGTTGCCTGT GGATGACAGA 
ACAAGTGCCA CTGACCATAA ATTTCCTTCT TTGATGGAAA ATTCAGATTC TCCGGTTAGA 
AGCATTCTGA AGTCCCAAGT CTGGCAGCCT TTGACTGAGG GCAGTGGAAA CAGAGGAATG 
TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA 
AAGTACGGGT CCTTTGAGGA AGTGGAGCCA CCCTGTCCTA CCCTGAACAG AGTCTGGGAA 
GGAGACAGCC ATAAGGAAAC CAAATATGCT GTTCCAAGAA AGGGTAGCCT GGAACTAGCT 
CACCCTCCCG TTGCCCACCT TGGCGATGAA CTGAAGGAGT TCTCCGTGGG TAAAAGCATT 
GCACAGGGGA GCCCAGACTG GACAGATAGG CAGCCCTTTG AAGAGAAGGT TGACCTGGAA 
AATGTCACGA GAAGGAGGTT TTCGCTAAGA GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG 
ACGGGAACAG CAGCCGGGAA AACCGTCACC CAGACTGCAG CCCCCATGTC CTGGAAGCAG 
CATGATTCTG TAGAACCAGT ACAGGAGAAG TACCCCAGGA ATCCATGTGC CATGTTTGCT 
GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG GGCATTCTGG ATTCAGCCAG CAAAACCATG 
TCAATTAAAG AAAGATTAGC GCTGTTGAAG AAGAGTGGTG AAGAAGATTG GAAGAACAGA 
CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG TCTGTCTCCA GCAGCCTGCA CATACAAGAA 
GCGGAACAGT CCCTCAAGAA GAAGCGGGTC ACAGAAAGTC GAGAGAGCCA GATGACTATC 
GAAGAGAGGA AGCATCTCAT CACTGTGAGA GAAGAAGCTT GGAAGACAAA AGGCAAAGGA 
GCGGCCAACG ACTCCACCCA GTTTACGGTG GCTGGCAGAA TGGTGAAGAA AGGTTTGGCA 
TCACCCACCG CCATAACTCC AGTAGCGTCT CCTATTTGCA GTAAAACGAG GGGCACCACA 
CCTGTTTCTA AACCTTTGGA AGATATTGAA GCCAGACCAG ACATGCAGTT GGAATCGGAC 
CTGAAGTTGG ACAGGCTGGA AACCTTTCTA AGAAGGCTGA ATAACAAAGT TGGTGGGATG 
CAGGAGACTG TACTCACTGT CACCGGTAAA TCTGTTAAAG AGGTGATGAA GTTGGATGAT 
GACGAGACCT TTGCCAGATT TTACCGCAGC ATGGATTATA CTGTGCCACG AAGCCCCGTG 
GAGCTAGACG AAGATTTCGA TGTCATTTTC GATCCTTATG CCCCTAAGTT GACCTCCTCT 
GTGGCCGAGC ACAAGCGCGC AGTTAGGCCC AAGCGCCGGG TTCAGGCTTC CAGAAACCCC 
TTGAAAATGC TGGCAGCCAG AGAAGATCTC CTTCAGGAGT ACACGGAGCA GAGACTAAAC 
ATTGCCTTCA TGGAGTCAAA GCGGATGAAA GTAGAAAAGA TGTCCTCCAA CTCCAACTTC 
TCAGAAGTCA CTCTGGCAGG CTTAGCCAGT AAAGAAAACT TCAGCAACAT CAGCCTGCGG 
AGCGTCAACC TGACGGAACA GAATTCCAAC AATAGCGCGG TACCCTATAA GAAGCTGATG 
TTGTTACAGA TTAAAGGAAG AAGACACGTG CAGACAAGAC TGGTCGAACC TCGAGCTTCA 
TCCCTCAACA GTGGGGACTG CTTCCTCCTG CTCTCCCCTC ACTACTGCTT CGTGTGGGTA 
GGAGAGTTTG CAAATGTCAT AGAAAAGGCA AAGGCCTCGG AACTTGCAAG TTTAATTCAG 
ACAAAGAGGG AACTTGGTTG TAGAGCTACA TATGTCCAAA CTATCGAAGA AGGAATTAAT 
ACACACACTC ATGCGGCCAA AGACTTCTGG AAGCTTCTGG GTGGCCAAAC CAGTTACCAG 
TCTGCCGGAG ACCCAAAAGA AGATGAACTT TATGAAACAG CCATAATTGA AACAAACTGC 
ATTTACCGTC TGATGGATGA CAAACTGGTC CCTGAGGACG ACTACTGGGG AAAGATTCCC 
AAGTGCTCCC TTCTGCAGTC AAAAGAGGTA CTGGTGTTTG ATTTTGGTAG TGAAGTGTAC 
GTGTGGCATG GAAAAGAAGT CACATTAGCG CAACGGAAAA TAGCTTTTCA ACTGGCAAAG 
CACTTATGGA ACGGAACCTT TGACTACGAG AACTGTGACA TCAACCCGTT GGATCCTGGA 
GAATGCAATC CGCTTATTCC CAGAAAAGGA CAAGGGCGAC CGGATTGGGC AATTTTCGGG 
AGACTTACAG AACACAATGA GACGATTTTG TTCAAAGAGA AATTTCTGGA TTGGACGGAA 
CTGAAGAGGC CTAATGAGAA GAGTGCCGGC GAACTCACCC AGCAAAAGGA AGATCCCAGG 
GCAGAAGCCA AGGCATATGA CGTGACACGG ATGGTGCCGG CACCCCAGGC CACAACCGGC 
ACTGTGCTGG ATGGGGTGAA TGTTGGTCGT GGCTACGGCT TGGTGGATGG GGATGACAGG 
AGGCAGTTTG AGATCGCCAG TGTGTCTGTG GATGTGTGGC ACATCCTAGA ATTTGACTAC 
AGCAGGCTAC CCAAACAGAG CATCGGGCAG TTCCACGAGG GAGACGCCTA TGTGGTCAAG 
TGGAAGTACA TGGTGAGCAC CGCAGTGGGA AGTCGACAAA AGGGAGAGCA CCCAGTACGG 
GTAGCTGGCA AGGAGAAGTG TGTCTACTTC TTCTGGCAAG GCCGGCAGTC GACCGTGAGT 
GAGAAGGGCA CATCAGCTCT GATGACAGTG GAGCTGGATG AGGAGAGGGG TGCCCAGGTC 
CAGGTTCTGC AGGGAAAGGA GCCCCCCTGC TTTCTGCAGT GTTTCCAGGG GGGAATGGTG 
GTACACTCTG GGAGACGGGA AGAGGAGGAA GAAAATGCAC AAAGTGAGTG GAGGCTGTAC 
TGTGTCCGAG GAGAAGTGCC CATGGAAGGA AATTTGCTGG AAGTGGCCTG TCACTGTAGT 
AGCCTGAGGT CCAGGACTTC CATGGTTGTC CTCAACATAA ACAAAGCCCT CATTTACTTA 
TGGCACGGAT GCAAAGCCCA AGCCCACACG AAGGAGGTTG GAAGGACTGC AGCAAATAAA 
ATCAAAGAAC AATGTCCCCT GGAAGCAGGA CTGCATAGTA GCAGCAAAGT GACAATACAC 
GAGTGTGACG AAGGATCCGA GCCGCTGGGA TTCTGGGATG CTTTAGGAAG GAGAGATAGA 
AAAGCGTACG ACTGTATGCT TCAAGATCCT GGAAATTTTA ACTTCACACC TCGCCTATTC 
ATCCTCAGCA GTTCCTCCGG TGACTTCTTA GCCACAGAGT TCATATATCC CGCCCGAGAC 
CCCTCTGTGG TCAACTCCAT GCCTTTCCTG CAGGAAGATC TTTACAGCGC GCCACAGCCA 
GCACTTTTCC TTGTTGACAA TCACCACGAG GTATACCTCT GGCAAGGCTG GTGGCCCATA 
GAGAACAAGA TCACTGGATC GGCCCGCATT CGCTGGGCCT CCGACCGGAA GAGCGCCATG 
GAAACCGTGC TCCAGTACTG CAAAGGAAAA AATATCAAGA GGCCGCCCCC CAAGTCTTAC 
CTTATCCATG CTGGTCTGGA GCCCTTGACA TTCACCAACA TGTTCCCCAG CTGGGAGCAC 
AGAGAGGACA TTGCTGAAAT CACAGAAATG GATACCGAAG TTTCAAATCA GATCACCCTT 
GTGGAAGATG TCTTAGCCAA GCTCTGTAAA ACCATCTATC CCCTGGCTGA TCTGCTAGCC 
AGGCCGCTAC CCGAGGGAGT TGATCCTCTG AAGCTTGAGA TTTATCTCAC CGATGAAGAC 
TTTGAGTTTG CACTAGACAT GACAAGAGAC GAATACAATG CTCTGCCCGC CTGGAAGCAG 
GTGAACCTGA AGAAAGCAAA GGGTCTGTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_022407080.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262788.1 Canis lupus familiaris supervillin (SVIL), transcript variant X16, mRNA.

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CloneID	OCe286615
Clone ID Related Accession (Same CDS sequence)	XM_022407080.1
Accession Version	XM_022407080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGGTCT 
GCTTCGCATT CAGAAATGCC AACCACTGAG GATGAAGAAA AGGTAGATGA ACGAGCCAAG 
CTGAGTGTCG CTGCCAAAAG GCTGCTTTTC AGGGAAATGG AAAAATCATT TGATGAGAAA 
AATGTTCCAA AGCGACGCTC CAGAAACGCG GCTGTGGAGC AAAGGCTGCG TCGGCTACAG 
GATAGATCCC ACACCCAGCC CGTTACCACC GAGGAAGTGG CCATTGCAGC CACTGAACCT 
ATCCCTGCTT CGTGCTCTGT GGCCACCCAC CCTGTAATGG CAAGACTTCC TAGTCCCACT 
GTAGCTAAGA GCACTGTGCA GCCTGCCAGG TTGCAGGCAT CTGCTCACCA CAAAGCCTTA 
GCCAGAGACC AGGCAAATGA GGGCAAAGAT GCTGCTGAAC AAGGTGAACC TGACTCCTCC 
ACTCTAAGCT TAGCAGAGAA GTTGGCCTTG TTTAACAAAT TATCCCAGCC AGTCTCAAAA 
GCTATTTCTA CCCGAAACAG AATAGACATG AGACAGAGGA GAATGAATGC TCGCTATCAG 
ACTCAGCCAG TCACGCTTGG AGAGGTGGAA CAGGTGCAAA GTGGAAAGCT CATTCCTTTC 
TCACCTACCG TTAACACATC TGTGTCCACC ATGGCATCTA CAGTCACTCC AATGTATGCA 
GGGGATCTTC GGACAAAGTT GCCTGTGGAT GACAGAACAA GTGCCACTGA CCATAAATTT 
CCTTCTTTGA TGGAAAATTC AGATTCTCCG GTTAGAAGCA TTCTGAAGTC CCAAGTCTGG 
CAGCCTTTGA CTGAGGGCAG TGGAAACAGA GGAATGTTGA GAGAGCTGGG CGAGACAGAA 
GGGAAGAGAG TCTTGGCAGG TGGAGATGGT GGTAAAAAGT ACGGGTCCTT TGAGGAAGTG 
GAGCCACCCT GTCCTACCCT GAACAGAGTC TGGGAAGGAG ACAGCCATAA GGAAACCAAA 
TATGCTGTTC CAAGAAAGGG TAGCCTGGAA CTAGCTCACC CTCCCGTTGC CCACCTTGGC 
GATGAACTGA AGGAGTTCTC CGTGGGTAAA AGCATTGCAC AGGGGAGCCC AGACTGGACA 
GATAGGCAGC CCTTTGAAGA GAAGGTTGAC CTGGAAAATG TCACGAGAAG GAGGTTTTCG 
CTAAGAGCAG AGTTTGGGGA GCCTGCTTCT GAGCAGACGG GAACAGCAGC CGGGAAAACC 
GTCACCCAGA CTGCAGCCCC CATGTCCTGG AAGCAGCATG ATTCTGTAGA ACCAGTACAG 
GAGAAGTACC CCAGGAATCC ATGTGCCATG TTTGCTGCCG GAGAGATCAA AGTGCCAGCG 
GTGGAGGGCA TTCTGGATTC AGCCAGCAAA ACCATGTCAA TTAAAGAAAG ATTAGCGCTG 
TTGAAGAAGA GTGGTGAAGA AGATTGGAAG AACAGACTGA GCAGAAAGCA GGAGTACGGC 
AAGGCGTCTG TCTCCAGCAG CCTGCACATA CAAGAAGCGG AACAGTCCCT CAAGAAGAAG 
GAAGAAGGAG GATTTGCAGA TGATAGTGCC ATTTCTAATC TGCTTTGGGA ACCTGTATAT 
GCTTCTACTT ATTCTCCTGC TATACCTGCT GCCCATAAAC ACCTGTCTTT TGTATCTATT 
AATCAGCGGG TCACAGAAAG TCGAGAGAGC CAGATGACTA TCGAAGAGAG GAAGCATCTC 
ATCACTGTGA GAGAAGAAGC TTGGAAGACA AAAGGCAAAG GAGCGGCCAA CGACTCCACC 
CAGTTTACGG TGGCTGGCAG AATGGTGAAG AAAGGTTTGG CATCACCCAC CGCCATAACT 
CCAGTAGCGT CTCCTATTTG CAGTAAAACG AGGGGCACCA CACCTGTTTC TAAACCTTTG 
GAAGATATTG AAGCCAGACC AGACATGCAG TTGGAATCGG ACCTGAAGTT GGACAGGCTG 
GAAACCTTTC TAAGAAGGCT GAATAACAAA GTTGGTGGGA TGCAGGAGAC TGTACTCACT 
GTCACCGGTA AATCTGTTAA AGAGGTGATG AAGTTGGATG ATGACGAGAC CTTTGCCAGA 
TTTTACCGCA GCATGGATTA TACTGTGCCA CGAAGCCCCG TGGAGCTAGA CGAAGATTTC 
GATGTCATTT TCGATCCTTA TGCCCCTAAG TTGACCTCCT CTGTGGCCGA GCACAAGCGC 
GCAGTTAGGC CCAAGCGCCG GGTTCAGGCT TCCAGAAACC CCTTGAAAAT GCTGGCAGCC 
AGAGAAGATC TCCTTCAGGA GTACACGGAG CAGAGACTAA ACATTGCCTT CATGGAGTCA 
AAGCGGATGA AAGTAGAAAA GATGTCCTCC AACTCCAACT TCTCAGAAGT CACTCTGGCA 
GGCTTAGCCA GTAAAGAAAA CTTCAGCAAC ATCAGCCTGC GGAGCGTCAA CCTGACGGAA 
CAGAATTCCA ACAATAGCGC GGTACCCTAT AAGAAGCTGA TGTTGTTACA GATTAAAGGA 
AGAAGACACG TGCAGACAAG ACTGGTCGAA CCTCGAGCTT CATCCCTCAA CAGTGGGGAC 
TGCTTCCTCC TGCTCTCCCC TCACTACTGC TTCGTGTGGG TAGGAGAGTT TGCAAATGTC 
ATAGAAAAGG CAAAGGCCTC GGAACTTGCA AGTTTAATTC AGACAAAGAG GGAACTTGGT 
TGTAGAGCTA CATATGTCCA AACTATCGAA GAAGGAATTA ATACACACAC TCATGCGGCC 
AAAGACTTCT GGAAGCTTCT GGGTGGCCAA ACCAGTTACC AGTCTGCCGG AGACCCAAAA 
GAAGATGAAC TTTATGAAAC AGCCATAATT GAAACAAACT GCATTTACCG TCTGATGGAT 
GACAAACTGG TCCCTGAGGA CGACTACTGG GGAAAGATTC CCAAGTGCTC CCTTCTGCAG 
TCAAAAGAGG TACTGGTGTT TGATTTTGGT AGTGAAGTGT ACGTGTGGCA TGGAAAAGAA 
GTCACATTAG CGCAACGGAA AATAGCTTTT CAACTGGCAA AGCACTTATG GAACGGAACC 
TTTGACTACG AGAACTGTGA CATCAACCCG TTGGATCCTG GAGAATGCAA TCCGCTTATT 
CCCAGAAAAG GACAAGGGCG ACCGGATTGG GCAATTTTCG GGAGACTTAC AGAACACAAT 
GAGACGATTT TGTTCAAAGA GAAATTTCTG GATTGGACGG AACTGAAGAG GCCTAATGAG 
AAGAGTGCCG GCGAACTCAC CCAGCAAAAG GAAGATCCCA GGGCAGAAGC CAAGGCATAT 
GACGTGACAC GGATGGTGCC GGCACCCCAG GCCACAACCG GCACTGTGCT GGATGGGGTG 
AATGTTGGTC GTGGCTACGG CTTGGTGGAT GGGGATGACA GGAGGCAGTT TGAGATCGCC 
AGTGTGTCTG TGGATGTGTG GCACATCCTA GAATTTGACT ACAGCAGGCT ACCCAAACAG 
AGCATCGGGC AGTTCCACGA GGGAGACGCC TATGTGGTCA AGTGGAAGTA CATGGTGAGC 
ACCGCAGTGG GAAGTCGACA AAAGGGAGAG CACCCAGTAC GGGTAGCTGG CAAGGAGAAG 
TGTGTCTACT TCTTCTGGCA AGGCCGGCAG TCGACCGTGA GTGAGAAGGG CACATCAGCT 
CTGATGACAG TGGAGCTGGA TGAGGAGAGG GGTGCCCAGG TCCAGGTTCT GCAGGGAAAG 
GAGCCCCCCT GCTTTCTGCA GTGTTTCCAG GGGGGAATGG TGGTACACTC TGGGAGACGG 
GAAGAGGAGG AAGAAAATGC ACAAAGTGAG TGGAGGCTGT ACTGTGTCCG AGGAGAAGTG 
CCCATGGAAG GAAATTTGCT GGAAGTGGCC TGTCACTGTA GTAGCCTGAG GTCCAGGACT 
TCCATGGTTG TCCTCAACAT AAACAAAGCC CTCATTTACT TATGGCACGG ATGCAAAGCC 
CAAGCCCACA CGAAGGAGGT TGGAAGGACT GCAGCAAATA AAATCAAAGA ACAATGTCCC 
CTGGAAGCAG GACTGCATAG TAGCAGCAAA GTGACAATAC ACGAGTGTGA CGAAGGATCC 
GAGCCGCTGG GATTCTGGGA TGCTTTAGGA AGGAGAGATA GAAAAGCGTA CGACTGTATG 
CTTCAAGATC CTGGAAATTT TAACTTCACA CCTCGCCTAT TCATCCTCAG CAGTTCCTCC 
GGTGACTTCT TAGCCACAGA GTTCATATAT CCCGCCCGAG ACCCCTCTGT GGTCAACTCC 
ATGCCTTTCC TGCAGGAAGA TCTTTACAGC GCGCCACAGC CAGCACTTTT CCTTGTTGAC 
AATCACCACG AGGTATACCT CTGGCAAGGC TGGTGGCCCA TAGAGAACAA GATCACTGGA 
TCGGCCCGCA TTCGCTGGGC CTCCGACCGG AAGAGCGCCA TGGAAACCGT GCTCCAGTAC 
TGCAAAGGAA AAAATATCAA GAGGCCGCCC CCCAAGTCTT ACCTTATCCA TGCTGGTCTG 
GAGCCCTTGA CATTCACCAA CATGTTCCCC AGCTGGGAGC ACAGAGAGGA CATTGCTGAA 
ATCACAGAAA TGGATACCGA AGTTTCAAAT CAGATCACCC TTGTGGAAGA TGTCTTAGCC 
AAGCTCTGTA AAACCATCTA TCCCCTGGCT GATCTGCTAG CCAGGCCGCT ACCCGAGGGA 
GTTGATCCTC TGAAGCTTGA GATTTATCTC ACCGATGAAG ACTTTGAGTT TGCACTAGAC 
ATGACAAGAG ACGAATACAA TGCTCTGCCC GCCTGGAAGC AGGTGAACCT GAAGAAAGCA 
AAGGGTCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262788.1
CDS	60..5894
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARARSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_022407080.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022407080.1

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGGTCT 
GCTTCGCATT CAGAAATGCC AACCACTGAG GATGAAGAAA AGGTAGATGA ACGAGCCAAG 
CTGAGTGTCG CTGCCAAAAG GCTGCTTTTC AGGGAAATGG AAAAATCATT TGATGAGAAA 
AATGTTCCAA AGCGACGCTC CAGAAACGCG GCTGTGGAGC AAAGGCTGCG TCGGCTACAG 
GATAGATCCC ACACCCAGCC CGTTACCACC GAGGAAGTGG CCATTGCAGC CACTGAACCT 
ATCCCTGCTT CGTGCTCTGT GGCCACCCAC CCTGTAATGG CAAGACTTCC TAGTCCCACT 
GTAGCTAAGA GCACTGTGCA GCCTGCCAGG TTGCAGGCAT CTGCTCACCA CAAAGCCTTA 
GCCAGAGACC AGGCAAATGA GGGCAAAGAT GCTGCTGAAC AAGGTGAACC TGACTCCTCC 
ACTCTAAGCT TAGCAGAGAA GTTGGCCTTG TTTAACAAAT TATCCCAGCC AGTCTCAAAA 
GCTATTTCTA CCCGAAACAG AATAGACATG AGACAGAGGA GAATGAATGC TCGCTATCAG 
ACTCAGCCAG TCACGCTTGG AGAGGTGGAA CAGGTGCAAA GTGGAAAGCT CATTCCTTTC 
TCACCTACCG TTAACACATC TGTGTCCACC ATGGCATCTA CAGTCACTCC AATGTATGCA 
GGGGATCTTC GGACAAAGTT GCCTGTGGAT GACAGAACAA GTGCCACTGA CCATAAATTT 
CCTTCTTTGA TGGAAAATTC AGATTCTCCG GTTAGAAGCA TTCTGAAGTC CCAAGTCTGG 
CAGCCTTTGA CTGAGGGCAG TGGAAACAGA GGAATGTTGA GAGAGCTGGG CGAGACAGAA 
GGGAAGAGAG TCTTGGCAGG TGGAGATGGT GGTAAAAAGT ACGGGTCCTT TGAGGAAGTG 
GAGCCACCCT GTCCTACCCT GAACAGAGTC TGGGAAGGAG ACAGCCATAA GGAAACCAAA 
TATGCTGTTC CAAGAAAGGG TAGCCTGGAA CTAGCTCACC CTCCCGTTGC CCACCTTGGC 
GATGAACTGA AGGAGTTCTC CGTGGGTAAA AGCATTGCAC AGGGGAGCCC AGACTGGACA 
GATAGGCAGC CCTTTGAAGA GAAGGTTGAC CTGGAAAATG TCACGAGAAG GAGGTTTTCG 
CTAAGAGCAG AGTTTGGGGA GCCTGCTTCT GAGCAGACGG GAACAGCAGC CGGGAAAACC 
GTCACCCAGA CTGCAGCCCC CATGTCCTGG AAGCAGCATG ATTCTGTAGA ACCAGTACAG 
GAGAAGTACC CCAGGAATCC ATGTGCCATG TTTGCTGCCG GAGAGATCAA AGTGCCAGCG 
GTGGAGGGCA TTCTGGATTC AGCCAGCAAA ACCATGTCAA TTAAAGAAAG ATTAGCGCTG 
TTGAAGAAGA GTGGTGAAGA AGATTGGAAG AACAGACTGA GCAGAAAGCA GGAGTACGGC 
AAGGCGTCTG TCTCCAGCAG CCTGCACATA CAAGAAGCGG AACAGTCCCT CAAGAAGAAG 
GAAGAAGGAG GATTTGCAGA TGATAGTGCC ATTTCTAATC TGCTTTGGGA ACCTGTATAT 
GCTTCTACTT ATTCTCCTGC TATACCTGCT GCCCATAAAC ACCTGTCTTT TGTATCTATT 
AATCAGCGGG TCACAGAAAG TCGAGAGAGC CAGATGACTA TCGAAGAGAG GAAGCATCTC 
ATCACTGTGA GAGAAGAAGC TTGGAAGACA AAAGGCAAAG GAGCGGCCAA CGACTCCACC 
CAGTTTACGG TGGCTGGCAG AATGGTGAAG AAAGGTTTGG CATCACCCAC CGCCATAACT 
CCAGTAGCGT CTCCTATTTG CAGTAAAACG AGGGGCACCA CACCTGTTTC TAAACCTTTG 
GAAGATATTG AAGCCAGACC AGACATGCAG TTGGAATCGG ACCTGAAGTT GGACAGGCTG 
GAAACCTTTC TAAGAAGGCT GAATAACAAA GTTGGTGGGA TGCAGGAGAC TGTACTCACT 
GTCACCGGTA AATCTGTTAA AGAGGTGATG AAGTTGGATG ATGACGAGAC CTTTGCCAGA 
TTTTACCGCA GCATGGATTA TACTGTGCCA CGAAGCCCCG TGGAGCTAGA CGAAGATTTC 
GATGTCATTT TCGATCCTTA TGCCCCTAAG TTGACCTCCT CTGTGGCCGA GCACAAGCGC 
GCAGTTAGGC CCAAGCGCCG GGTTCAGGCT TCCAGAAACC CCTTGAAAAT GCTGGCAGCC 
AGAGAAGATC TCCTTCAGGA GTACACGGAG CAGAGACTAA ACATTGCCTT CATGGAGTCA 
AAGCGGATGA AAGTAGAAAA GATGTCCTCC AACTCCAACT TCTCAGAAGT CACTCTGGCA 
GGCTTAGCCA GTAAAGAAAA CTTCAGCAAC ATCAGCCTGC GGAGCGTCAA CCTGACGGAA 
CAGAATTCCA ACAATAGCGC GGTACCCTAT AAGAAGCTGA TGTTGTTACA GATTAAAGGA 
AGAAGACACG TGCAGACAAG ACTGGTCGAA CCTCGAGCTT CATCCCTCAA CAGTGGGGAC 
TGCTTCCTCC TGCTCTCCCC TCACTACTGC TTCGTGTGGG TAGGAGAGTT TGCAAATGTC 
ATAGAAAAGG CAAAGGCCTC GGAACTTGCA AGTTTAATTC AGACAAAGAG GGAACTTGGT 
TGTAGAGCTA CATATGTCCA AACTATCGAA GAAGGAATTA ATACACACAC TCATGCGGCC 
AAAGACTTCT GGAAGCTTCT GGGTGGCCAA ACCAGTTACC AGTCTGCCGG AGACCCAAAA 
GAAGATGAAC TTTATGAAAC AGCCATAATT GAAACAAACT GCATTTACCG TCTGATGGAT 
GACAAACTGG TCCCTGAGGA CGACTACTGG GGAAAGATTC CCAAGTGCTC CCTTCTGCAG 
TCAAAAGAGG TACTGGTGTT TGATTTTGGT AGTGAAGTGT ACGTGTGGCA TGGAAAAGAA 
GTCACATTAG CGCAACGGAA AATAGCTTTT CAACTGGCAA AGCACTTATG GAACGGAACC 
TTTGACTACG AGAACTGTGA CATCAACCCG TTGGATCCTG GAGAATGCAA TCCGCTTATT 
CCCAGAAAAG GACAAGGGCG ACCGGATTGG GCAATTTTCG GGAGACTTAC AGAACACAAT 
GAGACGATTT TGTTCAAAGA GAAATTTCTG GATTGGACGG AACTGAAGAG GCCTAATGAG 
AAGAGTGCCG GCGAACTCAC CCAGCAAAAG GAAGATCCCA GGGCAGAAGC CAAGGCATAT 
GACGTGACAC GGATGGTGCC GGCACCCCAG GCCACAACCG GCACTGTGCT GGATGGGGTG 
AATGTTGGTC GTGGCTACGG CTTGGTGGAT GGGGATGACA GGAGGCAGTT TGAGATCGCC 
AGTGTGTCTG TGGATGTGTG GCACATCCTA GAATTTGACT ACAGCAGGCT ACCCAAACAG 
AGCATCGGGC AGTTCCACGA GGGAGACGCC TATGTGGTCA AGTGGAAGTA CATGGTGAGC 
ACCGCAGTGG GAAGTCGACA AAAGGGAGAG CACCCAGTAC GGGTAGCTGG CAAGGAGAAG 
TGTGTCTACT TCTTCTGGCA AGGCCGGCAG TCGACCGTGA GTGAGAAGGG CACATCAGCT 
CTGATGACAG TGGAGCTGGA TGAGGAGAGG GGTGCCCAGG TCCAGGTTCT GCAGGGAAAG 
GAGCCCCCCT GCTTTCTGCA GTGTTTCCAG GGGGGAATGG TGGTACACTC TGGGAGACGG 
GAAGAGGAGG AAGAAAATGC ACAAAGTGAG TGGAGGCTGT ACTGTGTCCG AGGAGAAGTG 
CCCATGGAAG GAAATTTGCT GGAAGTGGCC TGTCACTGTA GTAGCCTGAG GTCCAGGACT 
TCCATGGTTG TCCTCAACAT AAACAAAGCC CTCATTTACT TATGGCACGG ATGCAAAGCC 
CAAGCCCACA CGAAGGAGGT TGGAAGGACT GCAGCAAATA AAATCAAAGA ACAATGTCCC 
CTGGAAGCAG GACTGCATAG TAGCAGCAAA GTGACAATAC ACGAGTGTGA CGAAGGATCC 
GAGCCGCTGG GATTCTGGGA TGCTTTAGGA AGGAGAGATA GAAAAGCGTA CGACTGTATG 
CTTCAAGATC CTGGAAATTT TAACTTCACA CCTCGCCTAT TCATCCTCAG CAGTTCCTCC 
GGTGACTTCT TAGCCACAGA GTTCATATAT CCCGCCCGAG ACCCCTCTGT GGTCAACTCC 
ATGCCTTTCC TGCAGGAAGA TCTTTACAGC GCGCCACAGC CAGCACTTTT CCTTGTTGAC 
AATCACCACG AGGTATACCT CTGGCAAGGC TGGTGGCCCA TAGAGAACAA GATCACTGGA 
TCGGCCCGCA TTCGCTGGGC CTCCGACCGG AAGAGCGCCA TGGAAACCGT GCTCCAGTAC 
TGCAAAGGAA AAAATATCAA GAGGCCGCCC CCCAAGTCTT ACCTTATCCA TGCTGGTCTG 
GAGCCCTTGA CATTCACCAA CATGTTCCCC AGCTGGGAGC ACAGAGAGGA CATTGCTGAA 
ATCACAGAAA TGGATACCGA AGTTTCAAAT CAGATCACCC TTGTGGAAGA TGTCTTAGCC 
AAGCTCTGTA AAACCATCTA TCCCCTGGCT GATCTGCTAG CCAGGCCGCT ACCCGAGGGA 
GTTGATCCTC TGAAGCTTGA GATTTATCTC ACCGATGAAG ACTTTGAGTT TGCACTAGAC 
ATGACAAGAG ACGAATACAA TGCTCTGCCC GCCTGGAAGC AGGTGAACCT GAAGAAAGCA 
AAGGGTCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_022407082.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262790.1 Canis lupus familiaris supervillin (SVIL), transcript variant X17, mRNA.

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CloneID	OCe286616
Clone ID Related Accession (Same CDS sequence)	XM_022407082.1
Accession Version	XM_022407082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGGTCT 
GCTTCGCATT CAGAAATGCC AACCACTGAG GATGAAGAAA AGGTAGATGA ACGAGCCAAG 
CTGAGTGTCG CTGCCAAAAG GCTGCTTTTC AGGGAAATGG AAAAATCATT TGATGAGAAA 
AATGTTCCAA AGCGACGCTC CAGAAACGCG GCTGTGGAGC AAAGGCTGCG TCGGCTACAG 
GATAGATCCC ACACCCAGCC CGTTACCACC GAGGAAGTGG CCATTGCAGC CACTGAACCT 
ATCCCTGCTT CGTGCTCTGT GGCCACCCAC CCTGTAATGG CAAGACTTCC TAGTCCCACT 
GTAGCTAAGA GCACTGTGCA GCCTGCCAGG TTGCAGGCAT CTGCTCACCA CAAAGCCTTA 
GCCAGAGACC AGGCAAATGA GGGCAAAGAT GCTGCTGAAC AAGGTGAACC TGACTCCTCC 
ACTCTAAGCT TAGCAGAGAA GTTGGCCTTG TTTAACAAAT TATCCCAGCC AGTCTCAAAA 
GCTATTTCTA CCCGAAACAG AATAGACATG AGACAGAGGA GAATGAATGC TCGCTATCAG 
ACTCAGCCAG TCACGCTTGG AGAGGTGGAA CAGGTGCAAA GTGGAAAGCT CATTCCTTTC 
TCACCTACCG TTAACACATC TGTGTCCACC ATGGCATCTA CAGTCACTCC AATGTATGCA 
GGGGATCTTC GGACAAAGTT GCCTGTGGAT GACAGAACAA GTGCCACTGA CCATAAATTT 
CCTTCTTTGA TGGAAAATTC AGATTCTCCG GTTAGAAGCA TTCTGAAGTC CCAAGTCTGG 
CAGCCTTTGA CTGAGGGCAG TGGAAACAGA GGAATGTTGA GAGAGCTGGG CGAGACAGAA 
GGGAAGAGAG TCTTGGCAGG TGGAGATGGT GGTAAAAAGT ACGGGTCCTT TGAGGAAGTG 
GAGCCACCCT GTCCTACCCT GAACAGAGTC TGGGAAGGAG ACAGCCATAA GGAAACCAAA 
TATGCTGTTC CAAGAAAGGG TAGCCTGGAA CTAGCTCACC CTCCCGTTGC CCACCTTGGC 
GATGAACTGA AGGAGTTCTC CGTGGGTAAA AGCATTGCAC AGGGGAGCCC AGACTGGACA 
GATAGGCAGC CCTTTGAAGA GAAGGTTGAC CTGGAAAATG TCACGAGAAG GAGGTTTTCG 
CTAAGAGCAG AGTTTGGGGA GCCTGCTTCT GAGCAGACGG GAACAGCAGC CGGGAAAACC 
GTCACCCAGA CTGCAGCCCC CATGTCCTGG AAGCAGCATG ATTCTGTAGA ACCAGTACAG 
GAGAAGTACC CCAGGAATCC ATGTGCCATG TTTGCTGCCG GAGAGATCAA AGTGCCAGCG 
GTGGAGGGCA TTCTGGATTC AGCCAGCAAA ACCATGTCAA TTAAAGAAAG ATTAGCGCTG 
TTGAAGAAGA GTGGTGAAGA AGATTGGAAG AACAGACTGA GCAGAAAGCA GGAGTACGGC 
AAGGCGTCTG TCTCCAGCAG CCTGCACATA CAAGAAGCGG AACAGTCCCT CAAGAAGAAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262790.1
CDS	58..5766
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARARSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_022407082.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022407082.1

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGGTCT 
GCTTCGCATT CAGAAATGCC AACCACTGAG GATGAAGAAA AGGTAGATGA ACGAGCCAAG 
CTGAGTGTCG CTGCCAAAAG GCTGCTTTTC AGGGAAATGG AAAAATCATT TGATGAGAAA 
AATGTTCCAA AGCGACGCTC CAGAAACGCG GCTGTGGAGC AAAGGCTGCG TCGGCTACAG 
GATAGATCCC ACACCCAGCC CGTTACCACC GAGGAAGTGG CCATTGCAGC CACTGAACCT 
ATCCCTGCTT CGTGCTCTGT GGCCACCCAC CCTGTAATGG CAAGACTTCC TAGTCCCACT 
GTAGCTAAGA GCACTGTGCA GCCTGCCAGG TTGCAGGCAT CTGCTCACCA CAAAGCCTTA 
GCCAGAGACC AGGCAAATGA GGGCAAAGAT GCTGCTGAAC AAGGTGAACC TGACTCCTCC 
ACTCTAAGCT TAGCAGAGAA GTTGGCCTTG TTTAACAAAT TATCCCAGCC AGTCTCAAAA 
GCTATTTCTA CCCGAAACAG AATAGACATG AGACAGAGGA GAATGAATGC TCGCTATCAG 
ACTCAGCCAG TCACGCTTGG AGAGGTGGAA CAGGTGCAAA GTGGAAAGCT CATTCCTTTC 
TCACCTACCG TTAACACATC TGTGTCCACC ATGGCATCTA CAGTCACTCC AATGTATGCA 
GGGGATCTTC GGACAAAGTT GCCTGTGGAT GACAGAACAA GTGCCACTGA CCATAAATTT 
CCTTCTTTGA TGGAAAATTC AGATTCTCCG GTTAGAAGCA TTCTGAAGTC CCAAGTCTGG 
CAGCCTTTGA CTGAGGGCAG TGGAAACAGA GGAATGTTGA GAGAGCTGGG CGAGACAGAA 
GGGAAGAGAG TCTTGGCAGG TGGAGATGGT GGTAAAAAGT ACGGGTCCTT TGAGGAAGTG 
GAGCCACCCT GTCCTACCCT GAACAGAGTC TGGGAAGGAG ACAGCCATAA GGAAACCAAA 
TATGCTGTTC CAAGAAAGGG TAGCCTGGAA CTAGCTCACC CTCCCGTTGC CCACCTTGGC 
GATGAACTGA AGGAGTTCTC CGTGGGTAAA AGCATTGCAC AGGGGAGCCC AGACTGGACA 
GATAGGCAGC CCTTTGAAGA GAAGGTTGAC CTGGAAAATG TCACGAGAAG GAGGTTTTCG 
CTAAGAGCAG AGTTTGGGGA GCCTGCTTCT GAGCAGACGG GAACAGCAGC CGGGAAAACC 
GTCACCCAGA CTGCAGCCCC CATGTCCTGG AAGCAGCATG ATTCTGTAGA ACCAGTACAG 
GAGAAGTACC CCAGGAATCC ATGTGCCATG TTTGCTGCCG GAGAGATCAA AGTGCCAGCG 
GTGGAGGGCA TTCTGGATTC AGCCAGCAAA ACCATGTCAA TTAAAGAAAG ATTAGCGCTG 
TTGAAGAAGA GTGGTGAAGA AGATTGGAAG AACAGACTGA GCAGAAAGCA GGAGTACGGC 
AAGGCGTCTG TCTCCAGCAG CCTGCACATA CAAGAAGCGG AACAGTCCCT CAAGAAGAAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617045.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617102.1 Canis lupus familiaris supervillin (SVIL), transcript variant X1, mRNA.

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CloneID	OCe02719
Clone ID Related Accession (Same CDS sequence)	XM_005617045.3 , XM_005617045.2
Accession Version	XM_005617045.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACTG AACCTATCCC TGCTTCGTGC TCTGTGGCCA CCCACCCTGT AATGGCAAGA 
CTTCCTAGTC CCACTGTAGC TAAGAGCACT GTGCAGCCTG CCAGGTTGCA GGCATCTGCT 
CACCACAAAG CCTTAGCCAG AGACCAGGCA AATGAGGGCA AAGATGCTGC TGAACAAGGT 
GAACCTGACT CCTCCACTCT AAGCTTAGCA GAGAAGTTGG CCTTGTTTAA CAAATTATCC 
CAGCCAGTCT CAAAAGCTAT TTCTACCCGA AACAGAATAG ACATGAGACA GAGGAGAATG 
AATGCTCGCT ATCAGACTCA GCCAGTCACG CTTGGAGAGG TGGAACAGGT GCAAAGTGGA 
AAGCTCATTC CTTTCTCACC TACCGTTAAC ACATCTGTGT CCACCATGGC ATCTACAGTC 
ACTCCAATGT ATGCAGGGGA TCTTCGGACA AAGTTGCCTG TGGATGACAG AACAAGTGCC 
ACTGACCATA AATTTCCTTC TTTGATGGAA AATTCAGATT CTCCGGTTAG AAGCATTCTG 
AAGTCCCAAG TCTGGCAGCC TTTGACTGAG GGCAGTGGAA ACAGAGGAAT GTTGAGAGAG 
CTGGGCGAGA CAGAAGGGAA GAGAGTCTTG GCAGGTGGAG ATGGTGGTAA AAAGTACGGG 
TCCTTTGAGG AAGTGGAGCC ACCCTGTCCT ACCCTGAACA GAGTCTGGGA AGGAGACAGC 
CATAAGGAAA CCAAATATGC TGTTCCAAGA AAGGGTAGCC TGGAACTAGC TCACCCTCCC 
GTTGCCCACC TTGGCGATGA ACTGAAGGAG TTCTCCGTGG GTAAAAGCAT TGCACAGGGG 
AGCCCAGACT GGACAGATAG GCAGCCCTTT GAAGAGAAGG TTGACCTGGA AAATGTCACG 
AGAAGGAGGT TTTCGCTAAG AGCAGAGTTT GGGGAGCCTG CTTCTGAGCA GACGGGAACA 
GCAGCCGGGA AAACCGTCAC CCAGACTGCA GCCCCCATGT CCTGGAAGCA GCATGATTCT 
GTAGAACCAG TACAGGAGAA GTACCCCAGG AATCCATGTG CCATGTTTGC TGCCGGAGAG 
ATCAAAGTGC CAGCGGTGGA GGGCATTCTG GATTCAGCCA GCAAAACCAT GTCAATTAAA 
GAAAGATTAG CGCTGTTGAA GAAGAGTGGT GAAGAAGATT GGAAGAACAG ACTGAGCAGA 
AAGCAGGAGT ACGGCAAGGC GTCTGTCTCC AGCAGCCTGC ACATACAAGA AGCGGAACAG 
TCCCTCAAGA AGAAGGAAGA AGGAGGATTT GCAGATGATA GTGCCATTTC TAATCTGCTT 
TGGGAACCTG TATATGCTTC TACTTATTCT CCTGCTATAC CTGCTGCCCA TAAACACCTG 
TCTTTTGTAT CTATTAATCA GCGGGTCACA GAAAGTCGAG AGAGCCAGAT GACTATCGAA 
GAGAGGAAGC ATCTCATCAC TGTGAGAGAA GAAGCTTGGA AGACAAAAGG CAAAGGAGCG 
GCCAACGACT CCACCCAGTT TACGGTGGCT GGCAGAATGG TGAAGAAAGG TTTGGCATCA 
CCCACCGCCA TAACTCCAGT AGCGTCTCCT ATTTGCAGTA AAACGAGGGG CACCACACCT 
GTTTCTAAAC CTTTGGAAGA TATTGAAGCC AGACCAGACA TGCAGTTGGA ATCGGACCTG 
AAGTTGGACA GGCTGGAAAC CTTTCTAAGA AGGCTGAATA ACAAAGTTGG TGGGATGCAG 
GAGACTGTAC TCACTGTCAC CGGTAAATCT GTTAAAGAGG TGATGAAGTT GGATGATGAC 
GAGACCTTTG CCAGATTTTA CCGCAGCATG GATTATACTG TGCCACGAAG CCCCGTGGAG 
CTAGACGAAG ATTTCGATGT CATTTTCGAT CCTTATGCCC CTAAGTTGAC CTCCTCTGTG 
GCCGAGCACA AGCGCGCAGT TAGGCCCAAG CGCCGGGTTC AGGCTTCCAG AAACCCCTTG 
AAAATGCTGG CAGCCAGAGA AGATCTCCTT CAGGAGTACA CGGAGCAGAG ACTAAACATT 
GCCTTCATGG AGTCAAAGCG GATGAAAGTA GAAAAGATGT CCTCCAACTC CAACTTCTCA 
GAAGTCACTC TGGCAGGCTT AGCCAGTAAA GAAAACTTCA GCAACATCAG CCTGCGGAGC 
GTCAACCTGA CGGAACAGAA TTCCAACAAT AGCGCGGTAC CCTATAAGAA GCTGATGTTG 
TTACAGATTA AAGGAAGAAG ACACGTGCAG ACAAGACTGG TCGAACCTCG AGCTTCATCC 
CTCAACAGTG GGGACTGCTT CCTCCTGCTC TCCCCTCACT ACTGCTTCGT GTGGGTAGGA 
GAGTTTGCAA ATGTCATAGA AAAGGCAAAG GCCTCGGAAC TTGCAAGTTT AATTCAGACA 
AAGAGGGAAC TTGGTTGTAG AGCTACATAT GTCCAAACTA TCGAAGAAGG AATTAATACA 
CACACTCATG CGGCCAAAGA CTTCTGGAAG CTTCTGGGTG GCCAAACCAG TTACCAGTCT 
GCCGGAGACC CAAAAGAAGA TGAACTTTAT GAAACAGCCA TAATTGAAAC AAACTGCATT 
TACCGTCTGA TGGATGACAA ACTGGTCCCT GAGGACGACT ACTGGGGAAA GATTCCCAAG 
TGCTCCCTTC TGCAGTCAAA AGAGGTACTG GTGTTTGATT TTGGTAGTGA AGTGTACGTG 
TGGCATGGAA AAGAAGTCAC ATTAGCGCAA CGGAAAATAG CTTTTCAACT GGCAAAGCAC 
TTATGGAACG GAACCTTTGA CTACGAGAAC TGTGACATCA ACCCGTTGGA TCCTGGAGAA 
TGCAATCCGC TTATTCCCAG AAAAGGACAA GGGCGACCGG ATTGGGCAAT TTTCGGGAGA 
CTTACAGAAC ACAATGAGAC GATTTTGTTC AAAGAGAAAT TTCTGGATTG GACGGAACTG 
AAGAGGCCTA ATGAGAAGAG TGCCGGCGAA CTCACCCAGC AAAAGGAAGA TCCCAGGGCA 
GAAGCCAAGG CATATGACGT GACACGGATG GTGCCGGCAC CCCAGGCCAC AACCGGCACT 
GTGCTGGATG GGGTGAATGT TGGTCGTGGC TACGGCTTGG TGGATGGGGA TGACAGGAGG 
CAGTTTGAGA TCGCCAGTGT GTCTGTGGAT GTGTGGCACA TCCTAGAATT TGACTACAGC 
AGGCTACCCA AACAGAGCAT CGGGCAGTTC CACGAGGGAG ACGCCTATGT GGTCAAGTGG 
AAGTACATGG TGAGCACCGC AGTGGGAAGT CGACAAAAGG GAGAGCACCC AGTACGGGTA 
GCTGGCAAGG AGAAGTGTGT CTACTTCTTC TGGCAAGGCC GGCAGTCGAC CGTGAGTGAG 
AAGGGCACAT CAGCTCTGAT GACAGTGGAG CTGGATGAGG AGAGGGGTGC CCAGGTCCAG 
GTTCTGCAGG GAAAGGAGCC CCCCTGCTTT CTGCAGTGTT TCCAGGGGGG AATGGTGGTA 
CACTCTGGGA GACGGGAAGA GGAGGAAGAA AATGCACAAA GTGAGTGGAG GCTGTACTGT 
GTCCGAGGAG AAGTGCCCAT GGAAGGAAAT TTGCTGGAAG TGGCCTGTCA CTGTAGTAGC 
CTGAGGTCCA GGACTTCCAT GGTTGTCCTC AACATAAACA AAGCCCTCAT TTACTTATGG 
CACGGATGCA AAGCCCAAGC CCACACGAAG GAGGTTGGAA GGACTGCAGC AAATAAAATC 
AAAGAACAAT GTCCCCTGGA AGCAGGACTG CATAGTAGCA GCAAAGTGAC AATACACGAG 
TGTGACGAAG GATCCGAGCC GCTGGGATTC TGGGATGCTT TAGGAAGGAG AGATAGAAAA 
GCGTACGACT GTATGCTTCA AGATCCTGGA AATTTTAACT TCACACCTCG CCTATTCATC 
CTCAGCAGTT CCTCCGGTGA CTTCTTAGCC ACAGAGTTCA TATATCCCGC CCGAGACCCC 
TCTGTGGTCA ACTCCATGCC TTTCCTGCAG GAAGATCTTT ACAGCGCGCC ACAGCCAGCA 
CTTTTCCTTG TTGACAATCA CCACGAGGTA TACCTCTGGC AAGGCTGGTG GCCCATAGAG 
AACAAGATCA CTGGATCGGC CCGCATTCGC TGGGCCTCCG ACCGGAAGAG CGCCATGGAA 
ACCGTGCTCC AGTACTGCAA AGGAAAAAAT ATCAAGAGGC CGCCCCCCAA GTCTTACCTT 
ATCCATGCTG GTCTGGAGCC CTTGACATTC ACCAACATGT TCCCCAGCTG GGAGCACAGA 
GAGGACATTG CTGAAATCAC AGAAATGGAT ACCGAAGTTT CAAATCAGAT CACCCTTGTG 
GAAGATGTCT TAGCCAAGCT CTGTAAAACC ATCTATCCCC TGGCTGATCT GCTAGCCAGG 
CCGCTACCCG AGGGAGTTGA TCCTCTGAAG CTTGAGATTT ATCTCACCGA TGAAGACTTT 
GAGTTTGCAC TAGACATGAC AAGAGACGAA TACAATGCTC TGCCCGCCTG GAAGCAGGTG 
AACCTGAAGA AAGCAAAGGG TCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617102.1
CDS	66..7055
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617045.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617045.3

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACTG AACCTATCCC TGCTTCGTGC TCTGTGGCCA CCCACCCTGT AATGGCAAGA 
CTTCCTAGTC CCACTGTAGC TAAGAGCACT GTGCAGCCTG CCAGGTTGCA GGCATCTGCT 
CACCACAAAG CCTTAGCCAG AGACCAGGCA AATGAGGGCA AAGATGCTGC TGAACAAGGT 
GAACCTGACT CCTCCACTCT AAGCTTAGCA GAGAAGTTGG CCTTGTTTAA CAAATTATCC 
CAGCCAGTCT CAAAAGCTAT TTCTACCCGA AACAGAATAG ACATGAGACA GAGGAGAATG 
AATGCTCGCT ATCAGACTCA GCCAGTCACG CTTGGAGAGG TGGAACAGGT GCAAAGTGGA 
AAGCTCATTC CTTTCTCACC TACCGTTAAC ACATCTGTGT CCACCATGGC ATCTACAGTC 
ACTCCAATGT ATGCAGGGGA TCTTCGGACA AAGTTGCCTG TGGATGACAG AACAAGTGCC 
ACTGACCATA AATTTCCTTC TTTGATGGAA AATTCAGATT CTCCGGTTAG AAGCATTCTG 
AAGTCCCAAG TCTGGCAGCC TTTGACTGAG GGCAGTGGAA ACAGAGGAAT GTTGAGAGAG 
CTGGGCGAGA CAGAAGGGAA GAGAGTCTTG GCAGGTGGAG ATGGTGGTAA AAAGTACGGG 
TCCTTTGAGG AAGTGGAGCC ACCCTGTCCT ACCCTGAACA GAGTCTGGGA AGGAGACAGC 
CATAAGGAAA CCAAATATGC TGTTCCAAGA AAGGGTAGCC TGGAACTAGC TCACCCTCCC 
GTTGCCCACC TTGGCGATGA ACTGAAGGAG TTCTCCGTGG GTAAAAGCAT TGCACAGGGG 
AGCCCAGACT GGACAGATAG GCAGCCCTTT GAAGAGAAGG TTGACCTGGA AAATGTCACG 
AGAAGGAGGT TTTCGCTAAG AGCAGAGTTT GGGGAGCCTG CTTCTGAGCA GACGGGAACA 
GCAGCCGGGA AAACCGTCAC CCAGACTGCA GCCCCCATGT CCTGGAAGCA GCATGATTCT 
GTAGAACCAG TACAGGAGAA GTACCCCAGG AATCCATGTG CCATGTTTGC TGCCGGAGAG 
ATCAAAGTGC CAGCGGTGGA GGGCATTCTG GATTCAGCCA GCAAAACCAT GTCAATTAAA 
GAAAGATTAG CGCTGTTGAA GAAGAGTGGT GAAGAAGATT GGAAGAACAG ACTGAGCAGA 
AAGCAGGAGT ACGGCAAGGC GTCTGTCTCC AGCAGCCTGC ACATACAAGA AGCGGAACAG 
TCCCTCAAGA AGAAGGAAGA AGGAGGATTT GCAGATGATA GTGCCATTTC TAATCTGCTT 
TGGGAACCTG TATATGCTTC TACTTATTCT CCTGCTATAC CTGCTGCCCA TAAACACCTG 
TCTTTTGTAT CTATTAATCA GCGGGTCACA GAAAGTCGAG AGAGCCAGAT GACTATCGAA 
GAGAGGAAGC ATCTCATCAC TGTGAGAGAA GAAGCTTGGA AGACAAAAGG CAAAGGAGCG 
GCCAACGACT CCACCCAGTT TACGGTGGCT GGCAGAATGG TGAAGAAAGG TTTGGCATCA 
CCCACCGCCA TAACTCCAGT AGCGTCTCCT ATTTGCAGTA AAACGAGGGG CACCACACCT 
GTTTCTAAAC CTTTGGAAGA TATTGAAGCC AGACCAGACA TGCAGTTGGA ATCGGACCTG 
AAGTTGGACA GGCTGGAAAC CTTTCTAAGA AGGCTGAATA ACAAAGTTGG TGGGATGCAG 
GAGACTGTAC TCACTGTCAC CGGTAAATCT GTTAAAGAGG TGATGAAGTT GGATGATGAC 
GAGACCTTTG CCAGATTTTA CCGCAGCATG GATTATACTG TGCCACGAAG CCCCGTGGAG 
CTAGACGAAG ATTTCGATGT CATTTTCGAT CCTTATGCCC CTAAGTTGAC CTCCTCTGTG 
GCCGAGCACA AGCGCGCAGT TAGGCCCAAG CGCCGGGTTC AGGCTTCCAG AAACCCCTTG 
AAAATGCTGG CAGCCAGAGA AGATCTCCTT CAGGAGTACA CGGAGCAGAG ACTAAACATT 
GCCTTCATGG AGTCAAAGCG GATGAAAGTA GAAAAGATGT CCTCCAACTC CAACTTCTCA 
GAAGTCACTC TGGCAGGCTT AGCCAGTAAA GAAAACTTCA GCAACATCAG CCTGCGGAGC 
GTCAACCTGA CGGAACAGAA TTCCAACAAT AGCGCGGTAC CCTATAAGAA GCTGATGTTG 
TTACAGATTA AAGGAAGAAG ACACGTGCAG ACAAGACTGG TCGAACCTCG AGCTTCATCC 
CTCAACAGTG GGGACTGCTT CCTCCTGCTC TCCCCTCACT ACTGCTTCGT GTGGGTAGGA 
GAGTTTGCAA ATGTCATAGA AAAGGCAAAG GCCTCGGAAC TTGCAAGTTT AATTCAGACA 
AAGAGGGAAC TTGGTTGTAG AGCTACATAT GTCCAAACTA TCGAAGAAGG AATTAATACA 
CACACTCATG CGGCCAAAGA CTTCTGGAAG CTTCTGGGTG GCCAAACCAG TTACCAGTCT 
GCCGGAGACC CAAAAGAAGA TGAACTTTAT GAAACAGCCA TAATTGAAAC AAACTGCATT 
TACCGTCTGA TGGATGACAA ACTGGTCCCT GAGGACGACT ACTGGGGAAA GATTCCCAAG 
TGCTCCCTTC TGCAGTCAAA AGAGGTACTG GTGTTTGATT TTGGTAGTGA AGTGTACGTG 
TGGCATGGAA AAGAAGTCAC ATTAGCGCAA CGGAAAATAG CTTTTCAACT GGCAAAGCAC 
TTATGGAACG GAACCTTTGA CTACGAGAAC TGTGACATCA ACCCGTTGGA TCCTGGAGAA 
TGCAATCCGC TTATTCCCAG AAAAGGACAA GGGCGACCGG ATTGGGCAAT TTTCGGGAGA 
CTTACAGAAC ACAATGAGAC GATTTTGTTC AAAGAGAAAT TTCTGGATTG GACGGAACTG 
AAGAGGCCTA ATGAGAAGAG TGCCGGCGAA CTCACCCAGC AAAAGGAAGA TCCCAGGGCA 
GAAGCCAAGG CATATGACGT GACACGGATG GTGCCGGCAC CCCAGGCCAC AACCGGCACT 
GTGCTGGATG GGGTGAATGT TGGTCGTGGC TACGGCTTGG TGGATGGGGA TGACAGGAGG 
CAGTTTGAGA TCGCCAGTGT GTCTGTGGAT GTGTGGCACA TCCTAGAATT TGACTACAGC 
AGGCTACCCA AACAGAGCAT CGGGCAGTTC CACGAGGGAG ACGCCTATGT GGTCAAGTGG 
AAGTACATGG TGAGCACCGC AGTGGGAAGT CGACAAAAGG GAGAGCACCC AGTACGGGTA 
GCTGGCAAGG AGAAGTGTGT CTACTTCTTC TGGCAAGGCC GGCAGTCGAC CGTGAGTGAG 
AAGGGCACAT CAGCTCTGAT GACAGTGGAG CTGGATGAGG AGAGGGGTGC CCAGGTCCAG 
GTTCTGCAGG GAAAGGAGCC CCCCTGCTTT CTGCAGTGTT TCCAGGGGGG AATGGTGGTA 
CACTCTGGGA GACGGGAAGA GGAGGAAGAA AATGCACAAA GTGAGTGGAG GCTGTACTGT 
GTCCGAGGAG AAGTGCCCAT GGAAGGAAAT TTGCTGGAAG TGGCCTGTCA CTGTAGTAGC 
CTGAGGTCCA GGACTTCCAT GGTTGTCCTC AACATAAACA AAGCCCTCAT TTACTTATGG 
CACGGATGCA AAGCCCAAGC CCACACGAAG GAGGTTGGAA GGACTGCAGC AAATAAAATC 
AAAGAACAAT GTCCCCTGGA AGCAGGACTG CATAGTAGCA GCAAAGTGAC AATACACGAG 
TGTGACGAAG GATCCGAGCC GCTGGGATTC TGGGATGCTT TAGGAAGGAG AGATAGAAAA 
GCGTACGACT GTATGCTTCA AGATCCTGGA AATTTTAACT TCACACCTCG CCTATTCATC 
CTCAGCAGTT CCTCCGGTGA CTTCTTAGCC ACAGAGTTCA TATATCCCGC CCGAGACCCC 
TCTGTGGTCA ACTCCATGCC TTTCCTGCAG GAAGATCTTT ACAGCGCGCC ACAGCCAGCA 
CTTTTCCTTG TTGACAATCA CCACGAGGTA TACCTCTGGC AAGGCTGGTG GCCCATAGAG 
AACAAGATCA CTGGATCGGC CCGCATTCGC TGGGCCTCCG ACCGGAAGAG CGCCATGGAA 
ACCGTGCTCC AGTACTGCAA AGGAAAAAAT ATCAAGAGGC CGCCCCCCAA GTCTTACCTT 
ATCCATGCTG GTCTGGAGCC CTTGACATTC ACCAACATGT TCCCCAGCTG GGAGCACAGA 
GAGGACATTG CTGAAATCAC AGAAATGGAT ACCGAAGTTT CAAATCAGAT CACCCTTGTG 
GAAGATGTCT TAGCCAAGCT CTGTAAAACC ATCTATCCCC TGGCTGATCT GCTAGCCAGG 
CCGCTACCCG AGGGAGTTGA TCCTCTGAAG CTTGAGATTT ATCTCACCGA TGAAGACTTT 
GAGTTTGCAC TAGACATGAC AAGAGACGAA TACAATGCTC TGCCCGCCTG GAAGCAGGTG 
AACCTGAAGA AAGCAAAGGG TCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617045.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617102.1 Canis lupus familiaris supervillin (SVIL), transcript variant X1, mRNA.

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CloneID	OCe02719
Clone ID Related Accession (Same CDS sequence)	XM_005617045.3 , XM_005617045.2
Accession Version	XM_005617045.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	supervillin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726048.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACTG AACCTATCCC TGCTTCGTGC TCTGTGGCCA CCCACCCTGT AATGGCAAGA 
CTTCCTAGTC CCACTGTAGC TAAGAGCACT GTGCAGCCTG CCAGGTTGCA GGCATCTGCT 
CACCACAAAG CCTTAGCCAG AGACCAGGCA AATGAGGGCA AAGATGCTGC TGAACAAGGT 
GAACCTGACT CCTCCACTCT AAGCTTAGCA GAGAAGTTGG CCTTGTTTAA CAAATTATCC 
CAGCCAGTCT CAAAAGCTAT TTCTACCCGA AACAGAATAG ACATGAGACA GAGGAGAATG 
AATGCTCGCT ATCAGACTCA GCCAGTCACG CTTGGAGAGG TGGAACAGGT GCAAAGTGGA 
AAGCTCATTC CTTTCTCACC TACCGTTAAC ACATCTGTGT CCACCATGGC ATCTACAGTC 
ACTCCAATGT ATGCAGGGGA TCTTCGGACA AAGTTGCCTG TGGATGACAG AACAAGTGCC 
ACTGACCATA AATTTCCTTC TTTGATGGAA AATTCAGATT CTCCGGTTAG AAGCATTCTG 
AAGTCCCAAG TCTGGCAGCC TTTGACTGAG GGCAGTGGAA ACAGAGGAAT GTTGAGAGAG 
CTGGGCGAGA CAGAAGGGAA GAGAGTCTTG GCAGGTGGAG ATGGTGGTAA AAAGTACGGG 
TCCTTTGAGG AAGTGGAGCC ACCCTGTCCT ACCCTGAACA GAGTCTGGGA AGGAGACAGC 
CATAAGGAAA CCAAATATGC TGTTCCAAGA AAGGGTAGCC TGGAACTAGC TCACCCTCCC 
GTTGCCCACC TTGGCGATGA ACTGAAGGAG TTCTCCGTGG GTAAAAGCAT TGCACAGGGG 
AGCCCAGACT GGACAGATAG GCAGCCCTTT GAAGAGAAGG TTGACCTGGA AAATGTCACG 
AGAAGGAGGT TTTCGCTAAG AGCAGAGTTT GGGGAGCCTG CTTCTGAGCA GACGGGAACA 
GCAGCCGGGA AAACCGTCAC CCAGACTGCA GCCCCCATGT CCTGGAAGCA GCATGATTCT 
GTAGAACCAG TACAGGAGAA GTACCCCAGG AATCCATGTG CCATGTTTGC TGCCGGAGAG 
ATCAAAGTGC CAGCGGTGGA GGGCATTCTG GATTCAGCCA GCAAAACCAT GTCAATTAAA 
GAAAGATTAG CGCTGTTGAA GAAGAGTGGT GAAGAAGATT GGAAGAACAG ACTGAGCAGA 
AAGCAGGAGT ACGGCAAGGC GTCTGTCTCC AGCAGCCTGC ACATACAAGA AGCGGAACAG 
TCCCTCAAGA AGAAGGAAGA AGGAGGATTT GCAGATGATA GTGCCATTTC TAATCTGCTT 
TGGGAACCTG TATATGCTTC TACTTATTCT CCTGCTATAC CTGCTGCCCA TAAACACCTG 
TCTTTTGTAT CTATTAATCA GCGGGTCACA GAAAGTCGAG AGAGCCAGAT GACTATCGAA 
GAGAGGAAGC ATCTCATCAC TGTGAGAGAA GAAGCTTGGA AGACAAAAGG CAAAGGAGCG 
GCCAACGACT CCACCCAGTT TACGGTGGCT GGCAGAATGG TGAAGAAAGG TTTGGCATCA 
CCCACCGCCA TAACTCCAGT AGCGTCTCCT ATTTGCAGTA AAACGAGGGG CACCACACCT 
GTTTCTAAAC CTTTGGAAGA TATTGAAGCC AGACCAGACA TGCAGTTGGA ATCGGACCTG 
AAGTTGGACA GGCTGGAAAC CTTTCTAAGA AGGCTGAATA ACAAAGTTGG TGGGATGCAG 
GAGACTGTAC TCACTGTCAC CGGTAAATCT GTTAAAGAGG TGATGAAGTT GGATGATGAC 
GAGACCTTTG CCAGATTTTA CCGCAGCATG GATTATACTG TGCCACGAAG CCCCGTGGAG 
CTAGACGAAG ATTTCGATGT CATTTTCGAT CCTTATGCCC CTAAGTTGAC CTCCTCTGTG 
GCCGAGCACA AGCGCGCAGT TAGGCCCAAG CGCCGGGTTC AGGCTTCCAG AAACCCCTTG 
AAAATGCTGG CAGCCAGAGA AGATCTCCTT CAGGAGTACA CGGAGCAGAG ACTAAACATT 
GCCTTCATGG AGTCAAAGCG GATGAAAGTA GAAAAGATGT CCTCCAACTC CAACTTCTCA 
GAAGTCACTC TGGCAGGCTT AGCCAGTAAA GAAAACTTCA GCAACATCAG CCTGCGGAGC 
GTCAACCTGA CGGAACAGAA TTCCAACAAT AGCGCGGTAC CCTATAAGAA GCTGATGTTG 
TTACAGATTA AAGGAAGAAG ACACGTGCAG ACAAGACTGG TCGAACCTCG AGCTTCATCC 
CTCAACAGTG GGGACTGCTT CCTCCTGCTC TCCCCTCACT ACTGCTTCGT GTGGGTAGGA 
GAGTTTGCAA ATGTCATAGA AAAGGCAAAG GCCTCGGAAC TTGCAAGTTT AATTCAGACA 
AAGAGGGAAC TTGGTTGTAG AGCTACATAT GTCCAAACTA TCGAAGAAGG AATTAATACA 
CACACTCATG CGGCCAAAGA CTTCTGGAAG CTTCTGGGTG GCCAAACCAG TTACCAGTCT 
GCCGGAGACC CAAAAGAAGA TGAACTTTAT GAAACAGCCA TAATTGAAAC AAACTGCATT 
TACCGTCTGA TGGATGACAA ACTGGTCCCT GAGGACGACT ACTGGGGAAA GATTCCCAAG 
TGCTCCCTTC TGCAGTCAAA AGAGGTACTG GTGTTTGATT TTGGTAGTGA AGTGTACGTG 
TGGCATGGAA AAGAAGTCAC ATTAGCGCAA CGGAAAATAG CTTTTCAACT GGCAAAGCAC 
TTATGGAACG GAACCTTTGA CTACGAGAAC TGTGACATCA ACCCGTTGGA TCCTGGAGAA 
TGCAATCCGC TTATTCCCAG AAAAGGACAA GGGCGACCGG ATTGGGCAAT TTTCGGGAGA 
CTTACAGAAC ACAATGAGAC GATTTTGTTC AAAGAGAAAT TTCTGGATTG GACGGAACTG 
AAGAGGCCTA ATGAGAAGAG TGCCGGCGAA CTCACCCAGC AAAAGGAAGA TCCCAGGGCA 
GAAGCCAAGG CATATGACGT GACACGGATG GTGCCGGCAC CCCAGGCCAC AACCGGCACT 
GTGCTGGATG GGGTGAATGT TGGTCGTGGC TACGGCTTGG TGGATGGGGA TGACAGGAGG 
CAGTTTGAGA TCGCCAGTGT GTCTGTGGAT GTGTGGCACA TCCTAGAATT TGACTACAGC 
AGGCTACCCA AACAGAGCAT CGGGCAGTTC CACGAGGGAG ACGCCTATGT GGTCAAGTGG 
AAGTACATGG TGAGCACCGC AGTGGGAAGT CGACAAAAGG GAGAGCACCC AGTACGGGTA 
GCTGGCAAGG AGAAGTGTGT CTACTTCTTC TGGCAAGGCC GGCAGTCGAC CGTGAGTGAG 
AAGGGCACAT CAGCTCTGAT GACAGTGGAG CTGGATGAGG AGAGGGGTGC CCAGGTCCAG 
GTTCTGCAGG GAAAGGAGCC CCCCTGCTTT CTGCAGTGTT TCCAGGGGGG AATGGTGGTA 
CACTCTGGGA GACGGGAAGA GGAGGAAGAA AATGCACAAA GTGAGTGGAG GCTGTACTGT 
GTCCGAGGAG AAGTGCCCAT GGAAGGAAAT TTGCTGGAAG TGGCCTGTCA CTGTAGTAGC 
CTGAGGTCCA GGACTTCCAT GGTTGTCCTC AACATAAACA AAGCCCTCAT TTACTTATGG 
CACGGATGCA AAGCCCAAGC CCACACGAAG GAGGTTGGAA GGACTGCAGC AAATAAAATC 
AAAGAACAAT GTCCCCTGGA AGCAGGACTG CATAGTAGCA GCAAAGTGAC AATACACGAG 
TGTGACGAAG GATCCGAGCC GCTGGGATTC TGGGATGCTT TAGGAAGGAG AGATAGAAAA 
GCGTACGACT GTATGCTTCA AGATCCTGGA AATTTTAACT TCACACCTCG CCTATTCATC 
CTCAGCAGTT CCTCCGGTGA CTTCTTAGCC ACAGAGTTCA TATATCCCGC CCGAGACCCC 
TCTGTGGTCA ACTCCATGCC TTTCCTGCAG GAAGATCTTT ACAGCGCGCC ACAGCCAGCA 
CTTTTCCTTG TTGACAATCA CCACGAGGTA TACCTCTGGC AAGGCTGGTG GCCCATAGAG 
AACAAGATCA CTGGATCGGC CCGCATTCGC TGGGCCTCCG ACCGGAAGAG CGCCATGGAA 
ACCGTGCTCC AGTACTGCAA AGGAAAAAAT ATCAAGAGGC CGCCCCCCAA GTCTTACCTT 
ATCCATGCTG GTCTGGAGCC CTTGACATTC ACCAACATGT TCCCCAGCTG GGAGCACAGA 
GAGGACATTG CTGAAATCAC AGAAATGGAT ACCGAAGTTT CAAATCAGAT CACCCTTGTG 
GAAGATGTCT TAGCCAAGCT CTGTAAAACC ATCTATCCCC TGGCTGATCT GCTAGCCAGG 
CCGCTACCCG AGGGAGTTGA TCCTCTGAAG CTTGAGATTT ATCTCACCGA TGAAGACTTT 
GAGTTTGCAC TAGACATGAC AAGAGACGAA TACAATGCTC TGCCCGCCTG GAAGCAGGTG 
AACCTGAAGA AAGCAAAGGG TCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617102.1
CDS	49..7038
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617045.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617045.2

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACTG AACCTATCCC TGCTTCGTGC TCTGTGGCCA CCCACCCTGT AATGGCAAGA 
CTTCCTAGTC CCACTGTAGC TAAGAGCACT GTGCAGCCTG CCAGGTTGCA GGCATCTGCT 
CACCACAAAG CCTTAGCCAG AGACCAGGCA AATGAGGGCA AAGATGCTGC TGAACAAGGT 
GAACCTGACT CCTCCACTCT AAGCTTAGCA GAGAAGTTGG CCTTGTTTAA CAAATTATCC 
CAGCCAGTCT CAAAAGCTAT TTCTACCCGA AACAGAATAG ACATGAGACA GAGGAGAATG 
AATGCTCGCT ATCAGACTCA GCCAGTCACG CTTGGAGAGG TGGAACAGGT GCAAAGTGGA 
AAGCTCATTC CTTTCTCACC TACCGTTAAC ACATCTGTGT CCACCATGGC ATCTACAGTC 
ACTCCAATGT ATGCAGGGGA TCTTCGGACA AAGTTGCCTG TGGATGACAG AACAAGTGCC 
ACTGACCATA AATTTCCTTC TTTGATGGAA AATTCAGATT CTCCGGTTAG AAGCATTCTG 
AAGTCCCAAG TCTGGCAGCC TTTGACTGAG GGCAGTGGAA ACAGAGGAAT GTTGAGAGAG 
CTGGGCGAGA CAGAAGGGAA GAGAGTCTTG GCAGGTGGAG ATGGTGGTAA AAAGTACGGG 
TCCTTTGAGG AAGTGGAGCC ACCCTGTCCT ACCCTGAACA GAGTCTGGGA AGGAGACAGC 
CATAAGGAAA CCAAATATGC TGTTCCAAGA AAGGGTAGCC TGGAACTAGC TCACCCTCCC 
GTTGCCCACC TTGGCGATGA ACTGAAGGAG TTCTCCGTGG GTAAAAGCAT TGCACAGGGG 
AGCCCAGACT GGACAGATAG GCAGCCCTTT GAAGAGAAGG TTGACCTGGA AAATGTCACG 
AGAAGGAGGT TTTCGCTAAG AGCAGAGTTT GGGGAGCCTG CTTCTGAGCA GACGGGAACA 
GCAGCCGGGA AAACCGTCAC CCAGACTGCA GCCCCCATGT CCTGGAAGCA GCATGATTCT 
GTAGAACCAG TACAGGAGAA GTACCCCAGG AATCCATGTG CCATGTTTGC TGCCGGAGAG 
ATCAAAGTGC CAGCGGTGGA GGGCATTCTG GATTCAGCCA GCAAAACCAT GTCAATTAAA 
GAAAGATTAG CGCTGTTGAA GAAGAGTGGT GAAGAAGATT GGAAGAACAG ACTGAGCAGA 
AAGCAGGAGT ACGGCAAGGC GTCTGTCTCC AGCAGCCTGC ACATACAAGA AGCGGAACAG 
TCCCTCAAGA AGAAGGAAGA AGGAGGATTT GCAGATGATA GTGCCATTTC TAATCTGCTT 
TGGGAACCTG TATATGCTTC TACTTATTCT CCTGCTATAC CTGCTGCCCA TAAACACCTG 
TCTTTTGTAT CTATTAATCA GCGGGTCACA GAAAGTCGAG AGAGCCAGAT GACTATCGAA 
GAGAGGAAGC ATCTCATCAC TGTGAGAGAA GAAGCTTGGA AGACAAAAGG CAAAGGAGCG 
GCCAACGACT CCACCCAGTT TACGGTGGCT GGCAGAATGG TGAAGAAAGG TTTGGCATCA 
CCCACCGCCA TAACTCCAGT AGCGTCTCCT ATTTGCAGTA AAACGAGGGG CACCACACCT 
GTTTCTAAAC CTTTGGAAGA TATTGAAGCC AGACCAGACA TGCAGTTGGA ATCGGACCTG 
AAGTTGGACA GGCTGGAAAC CTTTCTAAGA AGGCTGAATA ACAAAGTTGG TGGGATGCAG 
GAGACTGTAC TCACTGTCAC CGGTAAATCT GTTAAAGAGG TGATGAAGTT GGATGATGAC 
GAGACCTTTG CCAGATTTTA CCGCAGCATG GATTATACTG TGCCACGAAG CCCCGTGGAG 
CTAGACGAAG ATTTCGATGT CATTTTCGAT CCTTATGCCC CTAAGTTGAC CTCCTCTGTG 
GCCGAGCACA AGCGCGCAGT TAGGCCCAAG CGCCGGGTTC AGGCTTCCAG AAACCCCTTG 
AAAATGCTGG CAGCCAGAGA AGATCTCCTT CAGGAGTACA CGGAGCAGAG ACTAAACATT 
GCCTTCATGG AGTCAAAGCG GATGAAAGTA GAAAAGATGT CCTCCAACTC CAACTTCTCA 
GAAGTCACTC TGGCAGGCTT AGCCAGTAAA GAAAACTTCA GCAACATCAG CCTGCGGAGC 
GTCAACCTGA CGGAACAGAA TTCCAACAAT AGCGCGGTAC CCTATAAGAA GCTGATGTTG 
TTACAGATTA AAGGAAGAAG ACACGTGCAG ACAAGACTGG TCGAACCTCG AGCTTCATCC 
CTCAACAGTG GGGACTGCTT CCTCCTGCTC TCCCCTCACT ACTGCTTCGT GTGGGTAGGA 
GAGTTTGCAA ATGTCATAGA AAAGGCAAAG GCCTCGGAAC TTGCAAGTTT AATTCAGACA 
AAGAGGGAAC TTGGTTGTAG AGCTACATAT GTCCAAACTA TCGAAGAAGG AATTAATACA 
CACACTCATG CGGCCAAAGA CTTCTGGAAG CTTCTGGGTG GCCAAACCAG TTACCAGTCT 
GCCGGAGACC CAAAAGAAGA TGAACTTTAT GAAACAGCCA TAATTGAAAC AAACTGCATT 
TACCGTCTGA TGGATGACAA ACTGGTCCCT GAGGACGACT ACTGGGGAAA GATTCCCAAG 
TGCTCCCTTC TGCAGTCAAA AGAGGTACTG GTGTTTGATT TTGGTAGTGA AGTGTACGTG 
TGGCATGGAA AAGAAGTCAC ATTAGCGCAA CGGAAAATAG CTTTTCAACT GGCAAAGCAC 
TTATGGAACG GAACCTTTGA CTACGAGAAC TGTGACATCA ACCCGTTGGA TCCTGGAGAA 
TGCAATCCGC TTATTCCCAG AAAAGGACAA GGGCGACCGG ATTGGGCAAT TTTCGGGAGA 
CTTACAGAAC ACAATGAGAC GATTTTGTTC AAAGAGAAAT TTCTGGATTG GACGGAACTG 
AAGAGGCCTA ATGAGAAGAG TGCCGGCGAA CTCACCCAGC AAAAGGAAGA TCCCAGGGCA 
GAAGCCAAGG CATATGACGT GACACGGATG GTGCCGGCAC CCCAGGCCAC AACCGGCACT 
GTGCTGGATG GGGTGAATGT TGGTCGTGGC TACGGCTTGG TGGATGGGGA TGACAGGAGG 
CAGTTTGAGA TCGCCAGTGT GTCTGTGGAT GTGTGGCACA TCCTAGAATT TGACTACAGC 
AGGCTACCCA AACAGAGCAT CGGGCAGTTC CACGAGGGAG ACGCCTATGT GGTCAAGTGG 
AAGTACATGG TGAGCACCGC AGTGGGAAGT CGACAAAAGG GAGAGCACCC AGTACGGGTA 
GCTGGCAAGG AGAAGTGTGT CTACTTCTTC TGGCAAGGCC GGCAGTCGAC CGTGAGTGAG 
AAGGGCACAT CAGCTCTGAT GACAGTGGAG CTGGATGAGG AGAGGGGTGC CCAGGTCCAG 
GTTCTGCAGG GAAAGGAGCC CCCCTGCTTT CTGCAGTGTT TCCAGGGGGG AATGGTGGTA 
CACTCTGGGA GACGGGAAGA GGAGGAAGAA AATGCACAAA GTGAGTGGAG GCTGTACTGT 
GTCCGAGGAG AAGTGCCCAT GGAAGGAAAT TTGCTGGAAG TGGCCTGTCA CTGTAGTAGC 
CTGAGGTCCA GGACTTCCAT GGTTGTCCTC AACATAAACA AAGCCCTCAT TTACTTATGG 
CACGGATGCA AAGCCCAAGC CCACACGAAG GAGGTTGGAA GGACTGCAGC AAATAAAATC 
AAAGAACAAT GTCCCCTGGA AGCAGGACTG CATAGTAGCA GCAAAGTGAC AATACACGAG 
TGTGACGAAG GATCCGAGCC GCTGGGATTC TGGGATGCTT TAGGAAGGAG AGATAGAAAA 
GCGTACGACT GTATGCTTCA AGATCCTGGA AATTTTAACT TCACACCTCG CCTATTCATC 
CTCAGCAGTT CCTCCGGTGA CTTCTTAGCC ACAGAGTTCA TATATCCCGC CCGAGACCCC 
TCTGTGGTCA ACTCCATGCC TTTCCTGCAG GAAGATCTTT ACAGCGCGCC ACAGCCAGCA 
CTTTTCCTTG TTGACAATCA CCACGAGGTA TACCTCTGGC AAGGCTGGTG GCCCATAGAG 
AACAAGATCA CTGGATCGGC CCGCATTCGC TGGGCCTCCG ACCGGAAGAG CGCCATGGAA 
ACCGTGCTCC AGTACTGCAA AGGAAAAAAT ATCAAGAGGC CGCCCCCCAA GTCTTACCTT 
ATCCATGCTG GTCTGGAGCC CTTGACATTC ACCAACATGT TCCCCAGCTG GGAGCACAGA 
GAGGACATTG CTGAAATCAC AGAAATGGAT ACCGAAGTTT CAAATCAGAT CACCCTTGTG 
GAAGATGTCT TAGCCAAGCT CTGTAAAACC ATCTATCCCC TGGCTGATCT GCTAGCCAGG 
CCGCTACCCG AGGGAGTTGA TCCTCTGAAG CTTGAGATTT ATCTCACCGA TGAAGACTTT 
GAGTTTGCAC TAGACATGAC AAGAGACGAA TACAATGCTC TGCCCGCCTG GAAGCAGGTG 
AACCTGAAGA AAGCAAAGGG TCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617046.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617103.1 Canis lupus familiaris supervillin (SVIL), transcript variant X2, mRNA.

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CloneID	OCe02720
Clone ID Related Accession (Same CDS sequence)	XM_005617046.3 , XM_005617046.2
Accession Version	XM_005617046.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617046.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACTG AACCTATCCC TGCTTCGTGC TCTGTGGCCA CCCACCCTGT AATGGCAAGA 
CTTCCTAGTC CCACTGTAGC TAAGAGCACT GTGCAGCCTG CCAGGTTGCA GGCATCTGCT 
CACCACAAAG CCTTAGCCAG AGACCAGGCA AATGAGGGCA AAGATGCTGC TGAACAAGGT 
GAACCTGACT CCTCCACTCT AAGCTTAGCA GAGAAGTTGG CCTTGTTTAA CAAATTATCC 
CAGCCAGTCT CAAAAGCTAT TTCTACCCGA AACAGAATAG ACATGAGACA GAGGAGAATG 
AATGCTCGCT ATCAGACTCA GCCAGTCACG CTTGGAGAGG TGGAACAGGT GCAAAGTGGA 
AAGCTCATTC CTTTCTCACC TACCGTTAAC ACATCTGTGT CCACCATGGC ATCTACAGTC 
ACTCCAATGT ATGCAGGGGA TCTTCGGACA AAGTTGCCTG TGGATGACAG AACAAGTGCC 
ACTGACCATA AATTTCCTTC TTTGATGGAA AATTCAGATT CTCCGGTTAG AAGCATTCTG 
AAGTCCCAAG TCTGGCAGCC TTTGACTGAG GGCAGTGGAA ACAGAGGAAT GTTGAGAGAG 
CTGGGCGAGA CAGAAGGGAA GAGAGTCTTG GCAGGTGGAG ATGGTGGTAA AAAGTACGGG 
TCCTTTGAGG AAGTGGAGCC ACCCTGTCCT ACCCTGAACA GAGTCTGGGA AGGAGACAGC 
CATAAGGAAA CCAAATATGC TGTTCCAAGA AAGGGTAGCC TGGAACTAGC TCACCCTCCC 
GTTGCCCACC TTGGCGATGA ACTGAAGGAG TTCTCCGTGG GTAAAAGCAT TGCACAGGGG 
AGCCCAGACT GGACAGATAG GCAGCCCTTT GAAGAGAAGG TTGACCTGGA AAATGTCACG 
AGAAGGAGGT TTTCGCTAAG AGAGTTTGGG GAGCCTGCTT CTGAGCAGAC GGGAACAGCA 
GCCGGGAAAA CCGTCACCCA GACTGCAGCC CCCATGTCCT GGAAGCAGCA TGATTCTGTA 
GAACCAGTAC AGGAGAAGTA CCCCAGGAAT CCATGTGCCA TGTTTGCTGC CGGAGAGATC 
AAAGTGCCAG CGGTGGAGGG CATTCTGGAT TCAGCCAGCA AAACCATGTC AATTAAAGAA 
AGATTAGCGC TGTTGAAGAA GAGTGGTGAA GAAGATTGGA AGAACAGACT GAGCAGAAAG 
CAGGAGTACG GCAAGGCGTC TGTCTCCAGC AGCCTGCACA TACAAGAAGC GGAACAGTCC 
CTCAAGAAGA AGGAAGAAGG AGGATTTGCA GATGATAGTG CCATTTCTAA TCTGCTTTGG 
GAACCTGTAT ATGCTTCTAC TTATTCTCCT GCTATACCTG CTGCCCATAA ACACCTGTCT 
TTTGTATCTA TTAATCAGCG GGTCACAGAA AGTCGAGAGA GCCAGATGAC TATCGAAGAG 
AGGAAGCATC TCATCACTGT GAGAGAAGAA GCTTGGAAGA CAAAAGGCAA AGGAGCGGCC 
AACGACTCCA CCCAGTTTAC GGTGGCTGGC AGAATGGTGA AGAAAGGTTT GGCATCACCC 
ACCGCCATAA CTCCAGTAGC GTCTCCTATT TGCAGTAAAA CGAGGGGCAC CACACCTGTT 
TCTAAACCTT TGGAAGATAT TGAAGCCAGA CCAGACATGC AGTTGGAATC GGACCTGAAG 
TTGGACAGGC TGGAAACCTT TCTAAGAAGG CTGAATAACA AAGTTGGTGG GATGCAGGAG 
ACTGTACTCA CTGTCACCGG TAAATCTGTT AAAGAGGTGA TGAAGTTGGA TGATGACGAG 
ACCTTTGCCA GATTTTACCG CAGCATGGAT TATACTGTGC CACGAAGCCC CGTGGAGCTA 
GACGAAGATT TCGATGTCAT TTTCGATCCT TATGCCCCTA AGTTGACCTC CTCTGTGGCC 
GAGCACAAGC GCGCAGTTAG GCCCAAGCGC CGGGTTCAGG CTTCCAGAAA CCCCTTGAAA 
ATGCTGGCAG CCAGAGAAGA TCTCCTTCAG GAGTACACGG AGCAGAGACT AAACATTGCC 
TTCATGGAGT CAAAGCGGAT GAAAGTAGAA AAGATGTCCT CCAACTCCAA CTTCTCAGAA 
GTCACTCTGG CAGGCTTAGC CAGTAAAGAA AACTTCAGCA ACATCAGCCT GCGGAGCGTC 
AACCTGACGG AACAGAATTC CAACAATAGC GCGGTACCCT ATAAGAAGCT GATGTTGTTA 
CAGATTAAAG GAAGAAGACA CGTGCAGACA AGACTGGTCG AACCTCGAGC TTCATCCCTC 
AACAGTGGGG ACTGCTTCCT CCTGCTCTCC CCTCACTACT GCTTCGTGTG GGTAGGAGAG 
TTTGCAAATG TCATAGAAAA GGCAAAGGCC TCGGAACTTG CAAGTTTAAT TCAGACAAAG 
AGGGAACTTG GTTGTAGAGC TACATATGTC CAAACTATCG AAGAAGGAAT TAATACACAC 
ACTCATGCGG CCAAAGACTT CTGGAAGCTT CTGGGTGGCC AAACCAGTTA CCAGTCTGCC 
GGAGACCCAA AAGAAGATGA ACTTTATGAA ACAGCCATAA TTGAAACAAA CTGCATTTAC 
CGTCTGATGG ATGACAAACT GGTCCCTGAG GACGACTACT GGGGAAAGAT TCCCAAGTGC 
TCCCTTCTGC AGTCAAAAGA GGTACTGGTG TTTGATTTTG GTAGTGAAGT GTACGTGTGG 
CATGGAAAAG AAGTCACATT AGCGCAACGG AAAATAGCTT TTCAACTGGC AAAGCACTTA 
TGGAACGGAA CCTTTGACTA CGAGAACTGT GACATCAACC CGTTGGATCC TGGAGAATGC 
AATCCGCTTA TTCCCAGAAA AGGACAAGGG CGACCGGATT GGGCAATTTT CGGGAGACTT 
ACAGAACACA ATGAGACGAT TTTGTTCAAA GAGAAATTTC TGGATTGGAC GGAACTGAAG 
AGGCCTAATG AGAAGAGTGC CGGCGAACTC ACCCAGCAAA AGGAAGATCC CAGGGCAGAA 
GCCAAGGCAT ATGACGTGAC ACGGATGGTG CCGGCACCCC AGGCCACAAC CGGCACTGTG 
CTGGATGGGG TGAATGTTGG TCGTGGCTAC GGCTTGGTGG ATGGGGATGA CAGGAGGCAG 
TTTGAGATCG CCAGTGTGTC TGTGGATGTG TGGCACATCC TAGAATTTGA CTACAGCAGG 
CTACCCAAAC AGAGCATCGG GCAGTTCCAC GAGGGAGACG CCTATGTGGT CAAGTGGAAG 
TACATGGTGA GCACCGCAGT GGGAAGTCGA CAAAAGGGAG AGCACCCAGT ACGGGTAGCT 
GGCAAGGAGA AGTGTGTCTA CTTCTTCTGG CAAGGCCGGC AGTCGACCGT GAGTGAGAAG 
GGCACATCAG CTCTGATGAC AGTGGAGCTG GATGAGGAGA GGGGTGCCCA GGTCCAGGTT 
CTGCAGGGAA AGGAGCCCCC CTGCTTTCTG CAGTGTTTCC AGGGGGGAAT GGTGGTACAC 
TCTGGGAGAC GGGAAGAGGA GGAAGAAAAT GCACAAAGTG AGTGGAGGCT GTACTGTGTC 
CGAGGAGAAG TGCCCATGGA AGGAAATTTG CTGGAAGTGG CCTGTCACTG TAGTAGCCTG 
AGGTCCAGGA CTTCCATGGT TGTCCTCAAC ATAAACAAAG CCCTCATTTA CTTATGGCAC 
GGATGCAAAG CCCAAGCCCA CACGAAGGAG GTTGGAAGGA CTGCAGCAAA TAAAATCAAA 
GAACAATGTC CCCTGGAAGC AGGACTGCAT AGTAGCAGCA AAGTGACAAT ACACGAGTGT 
GACGAAGGAT CCGAGCCGCT GGGATTCTGG GATGCTTTAG GAAGGAGAGA TAGAAAAGCG 
TACGACTGTA TGCTTCAAGA TCCTGGAAAT TTTAACTTCA CACCTCGCCT ATTCATCCTC 
AGCAGTTCCT CCGGTGACTT CTTAGCCACA GAGTTCATAT ATCCCGCCCG AGACCCCTCT 
GTGGTCAACT CCATGCCTTT CCTGCAGGAA GATCTTTACA GCGCGCCACA GCCAGCACTT 
TTCCTTGTTG ACAATCACCA CGAGGTATAC CTCTGGCAAG GCTGGTGGCC CATAGAGAAC 
AAGATCACTG GATCGGCCCG CATTCGCTGG GCCTCCGACC GGAAGAGCGC CATGGAAACC 
GTGCTCCAGT ACTGCAAAGG AAAAAATATC AAGAGGCCGC CCCCCAAGTC TTACCTTATC 
CATGCTGGTC TGGAGCCCTT GACATTCACC AACATGTTCC CCAGCTGGGA GCACAGAGAG 
GACATTGCTG AAATCACAGA AATGGATACC GAAGTTTCAA ATCAGATCAC CCTTGTGGAA 
GATGTCTTAG CCAAGCTCTG TAAAACCATC TATCCCCTGG CTGATCTGCT AGCCAGGCCG 
CTACCCGAGG GAGTTGATCC TCTGAAGCTT GAGATTTATC TCACCGATGA AGACTTTGAG 
TTTGCACTAG ACATGACAAG AGACGAATAC AATGCTCTGC CCGCCTGGAA GCAGGTGAAC 
CTGAAGAAAG CAAAGGGTCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617103.1
CDS	66..7052
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLREFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617046.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617046.3

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACTG AACCTATCCC TGCTTCGTGC TCTGTGGCCA CCCACCCTGT AATGGCAAGA 
CTTCCTAGTC CCACTGTAGC TAAGAGCACT GTGCAGCCTG CCAGGTTGCA GGCATCTGCT 
CACCACAAAG CCTTAGCCAG AGACCAGGCA AATGAGGGCA AAGATGCTGC TGAACAAGGT 
GAACCTGACT CCTCCACTCT AAGCTTAGCA GAGAAGTTGG CCTTGTTTAA CAAATTATCC 
CAGCCAGTCT CAAAAGCTAT TTCTACCCGA AACAGAATAG ACATGAGACA GAGGAGAATG 
AATGCTCGCT ATCAGACTCA GCCAGTCACG CTTGGAGAGG TGGAACAGGT GCAAAGTGGA 
AAGCTCATTC CTTTCTCACC TACCGTTAAC ACATCTGTGT CCACCATGGC ATCTACAGTC 
ACTCCAATGT ATGCAGGGGA TCTTCGGACA AAGTTGCCTG TGGATGACAG AACAAGTGCC 
ACTGACCATA AATTTCCTTC TTTGATGGAA AATTCAGATT CTCCGGTTAG AAGCATTCTG 
AAGTCCCAAG TCTGGCAGCC TTTGACTGAG GGCAGTGGAA ACAGAGGAAT GTTGAGAGAG 
CTGGGCGAGA CAGAAGGGAA GAGAGTCTTG GCAGGTGGAG ATGGTGGTAA AAAGTACGGG 
TCCTTTGAGG AAGTGGAGCC ACCCTGTCCT ACCCTGAACA GAGTCTGGGA AGGAGACAGC 
CATAAGGAAA CCAAATATGC TGTTCCAAGA AAGGGTAGCC TGGAACTAGC TCACCCTCCC 
GTTGCCCACC TTGGCGATGA ACTGAAGGAG TTCTCCGTGG GTAAAAGCAT TGCACAGGGG 
AGCCCAGACT GGACAGATAG GCAGCCCTTT GAAGAGAAGG TTGACCTGGA AAATGTCACG 
AGAAGGAGGT TTTCGCTAAG AGAGTTTGGG GAGCCTGCTT CTGAGCAGAC GGGAACAGCA 
GCCGGGAAAA CCGTCACCCA GACTGCAGCC CCCATGTCCT GGAAGCAGCA TGATTCTGTA 
GAACCAGTAC AGGAGAAGTA CCCCAGGAAT CCATGTGCCA TGTTTGCTGC CGGAGAGATC 
AAAGTGCCAG CGGTGGAGGG CATTCTGGAT TCAGCCAGCA AAACCATGTC AATTAAAGAA 
AGATTAGCGC TGTTGAAGAA GAGTGGTGAA GAAGATTGGA AGAACAGACT GAGCAGAAAG 
CAGGAGTACG GCAAGGCGTC TGTCTCCAGC AGCCTGCACA TACAAGAAGC GGAACAGTCC 
CTCAAGAAGA AGGAAGAAGG AGGATTTGCA GATGATAGTG CCATTTCTAA TCTGCTTTGG 
GAACCTGTAT ATGCTTCTAC TTATTCTCCT GCTATACCTG CTGCCCATAA ACACCTGTCT 
TTTGTATCTA TTAATCAGCG GGTCACAGAA AGTCGAGAGA GCCAGATGAC TATCGAAGAG 
AGGAAGCATC TCATCACTGT GAGAGAAGAA GCTTGGAAGA CAAAAGGCAA AGGAGCGGCC 
AACGACTCCA CCCAGTTTAC GGTGGCTGGC AGAATGGTGA AGAAAGGTTT GGCATCACCC 
ACCGCCATAA CTCCAGTAGC GTCTCCTATT TGCAGTAAAA CGAGGGGCAC CACACCTGTT 
TCTAAACCTT TGGAAGATAT TGAAGCCAGA CCAGACATGC AGTTGGAATC GGACCTGAAG 
TTGGACAGGC TGGAAACCTT TCTAAGAAGG CTGAATAACA AAGTTGGTGG GATGCAGGAG 
ACTGTACTCA CTGTCACCGG TAAATCTGTT AAAGAGGTGA TGAAGTTGGA TGATGACGAG 
ACCTTTGCCA GATTTTACCG CAGCATGGAT TATACTGTGC CACGAAGCCC CGTGGAGCTA 
GACGAAGATT TCGATGTCAT TTTCGATCCT TATGCCCCTA AGTTGACCTC CTCTGTGGCC 
GAGCACAAGC GCGCAGTTAG GCCCAAGCGC CGGGTTCAGG CTTCCAGAAA CCCCTTGAAA 
ATGCTGGCAG CCAGAGAAGA TCTCCTTCAG GAGTACACGG AGCAGAGACT AAACATTGCC 
TTCATGGAGT CAAAGCGGAT GAAAGTAGAA AAGATGTCCT CCAACTCCAA CTTCTCAGAA 
GTCACTCTGG CAGGCTTAGC CAGTAAAGAA AACTTCAGCA ACATCAGCCT GCGGAGCGTC 
AACCTGACGG AACAGAATTC CAACAATAGC GCGGTACCCT ATAAGAAGCT GATGTTGTTA 
CAGATTAAAG GAAGAAGACA CGTGCAGACA AGACTGGTCG AACCTCGAGC TTCATCCCTC 
AACAGTGGGG ACTGCTTCCT CCTGCTCTCC CCTCACTACT GCTTCGTGTG GGTAGGAGAG 
TTTGCAAATG TCATAGAAAA GGCAAAGGCC TCGGAACTTG CAAGTTTAAT TCAGACAAAG 
AGGGAACTTG GTTGTAGAGC TACATATGTC CAAACTATCG AAGAAGGAAT TAATACACAC 
ACTCATGCGG CCAAAGACTT CTGGAAGCTT CTGGGTGGCC AAACCAGTTA CCAGTCTGCC 
GGAGACCCAA AAGAAGATGA ACTTTATGAA ACAGCCATAA TTGAAACAAA CTGCATTTAC 
CGTCTGATGG ATGACAAACT GGTCCCTGAG GACGACTACT GGGGAAAGAT TCCCAAGTGC 
TCCCTTCTGC AGTCAAAAGA GGTACTGGTG TTTGATTTTG GTAGTGAAGT GTACGTGTGG 
CATGGAAAAG AAGTCACATT AGCGCAACGG AAAATAGCTT TTCAACTGGC AAAGCACTTA 
TGGAACGGAA CCTTTGACTA CGAGAACTGT GACATCAACC CGTTGGATCC TGGAGAATGC 
AATCCGCTTA TTCCCAGAAA AGGACAAGGG CGACCGGATT GGGCAATTTT CGGGAGACTT 
ACAGAACACA ATGAGACGAT TTTGTTCAAA GAGAAATTTC TGGATTGGAC GGAACTGAAG 
AGGCCTAATG AGAAGAGTGC CGGCGAACTC ACCCAGCAAA AGGAAGATCC CAGGGCAGAA 
GCCAAGGCAT ATGACGTGAC ACGGATGGTG CCGGCACCCC AGGCCACAAC CGGCACTGTG 
CTGGATGGGG TGAATGTTGG TCGTGGCTAC GGCTTGGTGG ATGGGGATGA CAGGAGGCAG 
TTTGAGATCG CCAGTGTGTC TGTGGATGTG TGGCACATCC TAGAATTTGA CTACAGCAGG 
CTACCCAAAC AGAGCATCGG GCAGTTCCAC GAGGGAGACG CCTATGTGGT CAAGTGGAAG 
TACATGGTGA GCACCGCAGT GGGAAGTCGA CAAAAGGGAG AGCACCCAGT ACGGGTAGCT 
GGCAAGGAGA AGTGTGTCTA CTTCTTCTGG CAAGGCCGGC AGTCGACCGT GAGTGAGAAG 
GGCACATCAG CTCTGATGAC AGTGGAGCTG GATGAGGAGA GGGGTGCCCA GGTCCAGGTT 
CTGCAGGGAA AGGAGCCCCC CTGCTTTCTG CAGTGTTTCC AGGGGGGAAT GGTGGTACAC 
TCTGGGAGAC GGGAAGAGGA GGAAGAAAAT GCACAAAGTG AGTGGAGGCT GTACTGTGTC 
CGAGGAGAAG TGCCCATGGA AGGAAATTTG CTGGAAGTGG CCTGTCACTG TAGTAGCCTG 
AGGTCCAGGA CTTCCATGGT TGTCCTCAAC ATAAACAAAG CCCTCATTTA CTTATGGCAC 
GGATGCAAAG CCCAAGCCCA CACGAAGGAG GTTGGAAGGA CTGCAGCAAA TAAAATCAAA 
GAACAATGTC CCCTGGAAGC AGGACTGCAT AGTAGCAGCA AAGTGACAAT ACACGAGTGT 
GACGAAGGAT CCGAGCCGCT GGGATTCTGG GATGCTTTAG GAAGGAGAGA TAGAAAAGCG 
TACGACTGTA TGCTTCAAGA TCCTGGAAAT TTTAACTTCA CACCTCGCCT ATTCATCCTC 
AGCAGTTCCT CCGGTGACTT CTTAGCCACA GAGTTCATAT ATCCCGCCCG AGACCCCTCT 
GTGGTCAACT CCATGCCTTT CCTGCAGGAA GATCTTTACA GCGCGCCACA GCCAGCACTT 
TTCCTTGTTG ACAATCACCA CGAGGTATAC CTCTGGCAAG GCTGGTGGCC CATAGAGAAC 
AAGATCACTG GATCGGCCCG CATTCGCTGG GCCTCCGACC GGAAGAGCGC CATGGAAACC 
GTGCTCCAGT ACTGCAAAGG AAAAAATATC AAGAGGCCGC CCCCCAAGTC TTACCTTATC 
CATGCTGGTC TGGAGCCCTT GACATTCACC AACATGTTCC CCAGCTGGGA GCACAGAGAG 
GACATTGCTG AAATCACAGA AATGGATACC GAAGTTTCAA ATCAGATCAC CCTTGTGGAA 
GATGTCTTAG CCAAGCTCTG TAAAACCATC TATCCCCTGG CTGATCTGCT AGCCAGGCCG 
CTACCCGAGG GAGTTGATCC TCTGAAGCTT GAGATTTATC TCACCGATGA AGACTTTGAG 
TTTGCACTAG ACATGACAAG AGACGAATAC AATGCTCTGC CCGCCTGGAA GCAGGTGAAC 
CTGAAGAAAG CAAAGGGTCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617046.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617103.1 Canis lupus familiaris supervillin (SVIL), transcript variant X2, mRNA.

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CloneID	OCe02720
Clone ID Related Accession (Same CDS sequence)	XM_005617046.3 , XM_005617046.2
Accession Version	XM_005617046.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	supervillin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726048.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617046.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACTG AACCTATCCC TGCTTCGTGC TCTGTGGCCA CCCACCCTGT AATGGCAAGA 
CTTCCTAGTC CCACTGTAGC TAAGAGCACT GTGCAGCCTG CCAGGTTGCA GGCATCTGCT 
CACCACAAAG CCTTAGCCAG AGACCAGGCA AATGAGGGCA AAGATGCTGC TGAACAAGGT 
GAACCTGACT CCTCCACTCT AAGCTTAGCA GAGAAGTTGG CCTTGTTTAA CAAATTATCC 
CAGCCAGTCT CAAAAGCTAT TTCTACCCGA AACAGAATAG ACATGAGACA GAGGAGAATG 
AATGCTCGCT ATCAGACTCA GCCAGTCACG CTTGGAGAGG TGGAACAGGT GCAAAGTGGA 
AAGCTCATTC CTTTCTCACC TACCGTTAAC ACATCTGTGT CCACCATGGC ATCTACAGTC 
ACTCCAATGT ATGCAGGGGA TCTTCGGACA AAGTTGCCTG TGGATGACAG AACAAGTGCC 
ACTGACCATA AATTTCCTTC TTTGATGGAA AATTCAGATT CTCCGGTTAG AAGCATTCTG 
AAGTCCCAAG TCTGGCAGCC TTTGACTGAG GGCAGTGGAA ACAGAGGAAT GTTGAGAGAG 
CTGGGCGAGA CAGAAGGGAA GAGAGTCTTG GCAGGTGGAG ATGGTGGTAA AAAGTACGGG 
TCCTTTGAGG AAGTGGAGCC ACCCTGTCCT ACCCTGAACA GAGTCTGGGA AGGAGACAGC 
CATAAGGAAA CCAAATATGC TGTTCCAAGA AAGGGTAGCC TGGAACTAGC TCACCCTCCC 
GTTGCCCACC TTGGCGATGA ACTGAAGGAG TTCTCCGTGG GTAAAAGCAT TGCACAGGGG 
AGCCCAGACT GGACAGATAG GCAGCCCTTT GAAGAGAAGG TTGACCTGGA AAATGTCACG 
AGAAGGAGGT TTTCGCTAAG AGAGTTTGGG GAGCCTGCTT CTGAGCAGAC GGGAACAGCA 
GCCGGGAAAA CCGTCACCCA GACTGCAGCC CCCATGTCCT GGAAGCAGCA TGATTCTGTA 
GAACCAGTAC AGGAGAAGTA CCCCAGGAAT CCATGTGCCA TGTTTGCTGC CGGAGAGATC 
AAAGTGCCAG CGGTGGAGGG CATTCTGGAT TCAGCCAGCA AAACCATGTC AATTAAAGAA 
AGATTAGCGC TGTTGAAGAA GAGTGGTGAA GAAGATTGGA AGAACAGACT GAGCAGAAAG 
CAGGAGTACG GCAAGGCGTC TGTCTCCAGC AGCCTGCACA TACAAGAAGC GGAACAGTCC 
CTCAAGAAGA AGGAAGAAGG AGGATTTGCA GATGATAGTG CCATTTCTAA TCTGCTTTGG 
GAACCTGTAT ATGCTTCTAC TTATTCTCCT GCTATACCTG CTGCCCATAA ACACCTGTCT 
TTTGTATCTA TTAATCAGCG GGTCACAGAA AGTCGAGAGA GCCAGATGAC TATCGAAGAG 
AGGAAGCATC TCATCACTGT GAGAGAAGAA GCTTGGAAGA CAAAAGGCAA AGGAGCGGCC 
AACGACTCCA CCCAGTTTAC GGTGGCTGGC AGAATGGTGA AGAAAGGTTT GGCATCACCC 
ACCGCCATAA CTCCAGTAGC GTCTCCTATT TGCAGTAAAA CGAGGGGCAC CACACCTGTT 
TCTAAACCTT TGGAAGATAT TGAAGCCAGA CCAGACATGC AGTTGGAATC GGACCTGAAG 
TTGGACAGGC TGGAAACCTT TCTAAGAAGG CTGAATAACA AAGTTGGTGG GATGCAGGAG 
ACTGTACTCA CTGTCACCGG TAAATCTGTT AAAGAGGTGA TGAAGTTGGA TGATGACGAG 
ACCTTTGCCA GATTTTACCG CAGCATGGAT TATACTGTGC CACGAAGCCC CGTGGAGCTA 
GACGAAGATT TCGATGTCAT TTTCGATCCT TATGCCCCTA AGTTGACCTC CTCTGTGGCC 
GAGCACAAGC GCGCAGTTAG GCCCAAGCGC CGGGTTCAGG CTTCCAGAAA CCCCTTGAAA 
ATGCTGGCAG CCAGAGAAGA TCTCCTTCAG GAGTACACGG AGCAGAGACT AAACATTGCC 
TTCATGGAGT CAAAGCGGAT GAAAGTAGAA AAGATGTCCT CCAACTCCAA CTTCTCAGAA 
GTCACTCTGG CAGGCTTAGC CAGTAAAGAA AACTTCAGCA ACATCAGCCT GCGGAGCGTC 
AACCTGACGG AACAGAATTC CAACAATAGC GCGGTACCCT ATAAGAAGCT GATGTTGTTA 
CAGATTAAAG GAAGAAGACA CGTGCAGACA AGACTGGTCG AACCTCGAGC TTCATCCCTC 
AACAGTGGGG ACTGCTTCCT CCTGCTCTCC CCTCACTACT GCTTCGTGTG GGTAGGAGAG 
TTTGCAAATG TCATAGAAAA GGCAAAGGCC TCGGAACTTG CAAGTTTAAT TCAGACAAAG 
AGGGAACTTG GTTGTAGAGC TACATATGTC CAAACTATCG AAGAAGGAAT TAATACACAC 
ACTCATGCGG CCAAAGACTT CTGGAAGCTT CTGGGTGGCC AAACCAGTTA CCAGTCTGCC 
GGAGACCCAA AAGAAGATGA ACTTTATGAA ACAGCCATAA TTGAAACAAA CTGCATTTAC 
CGTCTGATGG ATGACAAACT GGTCCCTGAG GACGACTACT GGGGAAAGAT TCCCAAGTGC 
TCCCTTCTGC AGTCAAAAGA GGTACTGGTG TTTGATTTTG GTAGTGAAGT GTACGTGTGG 
CATGGAAAAG AAGTCACATT AGCGCAACGG AAAATAGCTT TTCAACTGGC AAAGCACTTA 
TGGAACGGAA CCTTTGACTA CGAGAACTGT GACATCAACC CGTTGGATCC TGGAGAATGC 
AATCCGCTTA TTCCCAGAAA AGGACAAGGG CGACCGGATT GGGCAATTTT CGGGAGACTT 
ACAGAACACA ATGAGACGAT TTTGTTCAAA GAGAAATTTC TGGATTGGAC GGAACTGAAG 
AGGCCTAATG AGAAGAGTGC CGGCGAACTC ACCCAGCAAA AGGAAGATCC CAGGGCAGAA 
GCCAAGGCAT ATGACGTGAC ACGGATGGTG CCGGCACCCC AGGCCACAAC CGGCACTGTG 
CTGGATGGGG TGAATGTTGG TCGTGGCTAC GGCTTGGTGG ATGGGGATGA CAGGAGGCAG 
TTTGAGATCG CCAGTGTGTC TGTGGATGTG TGGCACATCC TAGAATTTGA CTACAGCAGG 
CTACCCAAAC AGAGCATCGG GCAGTTCCAC GAGGGAGACG CCTATGTGGT CAAGTGGAAG 
TACATGGTGA GCACCGCAGT GGGAAGTCGA CAAAAGGGAG AGCACCCAGT ACGGGTAGCT 
GGCAAGGAGA AGTGTGTCTA CTTCTTCTGG CAAGGCCGGC AGTCGACCGT GAGTGAGAAG 
GGCACATCAG CTCTGATGAC AGTGGAGCTG GATGAGGAGA GGGGTGCCCA GGTCCAGGTT 
CTGCAGGGAA AGGAGCCCCC CTGCTTTCTG CAGTGTTTCC AGGGGGGAAT GGTGGTACAC 
TCTGGGAGAC GGGAAGAGGA GGAAGAAAAT GCACAAAGTG AGTGGAGGCT GTACTGTGTC 
CGAGGAGAAG TGCCCATGGA AGGAAATTTG CTGGAAGTGG CCTGTCACTG TAGTAGCCTG 
AGGTCCAGGA CTTCCATGGT TGTCCTCAAC ATAAACAAAG CCCTCATTTA CTTATGGCAC 
GGATGCAAAG CCCAAGCCCA CACGAAGGAG GTTGGAAGGA CTGCAGCAAA TAAAATCAAA 
GAACAATGTC CCCTGGAAGC AGGACTGCAT AGTAGCAGCA AAGTGACAAT ACACGAGTGT 
GACGAAGGAT CCGAGCCGCT GGGATTCTGG GATGCTTTAG GAAGGAGAGA TAGAAAAGCG 
TACGACTGTA TGCTTCAAGA TCCTGGAAAT TTTAACTTCA CACCTCGCCT ATTCATCCTC 
AGCAGTTCCT CCGGTGACTT CTTAGCCACA GAGTTCATAT ATCCCGCCCG AGACCCCTCT 
GTGGTCAACT CCATGCCTTT CCTGCAGGAA GATCTTTACA GCGCGCCACA GCCAGCACTT 
TTCCTTGTTG ACAATCACCA CGAGGTATAC CTCTGGCAAG GCTGGTGGCC CATAGAGAAC 
AAGATCACTG GATCGGCCCG CATTCGCTGG GCCTCCGACC GGAAGAGCGC CATGGAAACC 
GTGCTCCAGT ACTGCAAAGG AAAAAATATC AAGAGGCCGC CCCCCAAGTC TTACCTTATC 
CATGCTGGTC TGGAGCCCTT GACATTCACC AACATGTTCC CCAGCTGGGA GCACAGAGAG 
GACATTGCTG AAATCACAGA AATGGATACC GAAGTTTCAA ATCAGATCAC CCTTGTGGAA 
GATGTCTTAG CCAAGCTCTG TAAAACCATC TATCCCCTGG CTGATCTGCT AGCCAGGCCG 
CTACCCGAGG GAGTTGATCC TCTGAAGCTT GAGATTTATC TCACCGATGA AGACTTTGAG 
TTTGCACTAG ACATGACAAG AGACGAATAC AATGCTCTGC CCGCCTGGAA GCAGGTGAAC 
CTGAAGAAAG CAAAGGGTCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617103.1
CDS	48..7034
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLREFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617046.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617046.2

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACTG AACCTATCCC TGCTTCGTGC TCTGTGGCCA CCCACCCTGT AATGGCAAGA 
CTTCCTAGTC CCACTGTAGC TAAGAGCACT GTGCAGCCTG CCAGGTTGCA GGCATCTGCT 
CACCACAAAG CCTTAGCCAG AGACCAGGCA AATGAGGGCA AAGATGCTGC TGAACAAGGT 
GAACCTGACT CCTCCACTCT AAGCTTAGCA GAGAAGTTGG CCTTGTTTAA CAAATTATCC 
CAGCCAGTCT CAAAAGCTAT TTCTACCCGA AACAGAATAG ACATGAGACA GAGGAGAATG 
AATGCTCGCT ATCAGACTCA GCCAGTCACG CTTGGAGAGG TGGAACAGGT GCAAAGTGGA 
AAGCTCATTC CTTTCTCACC TACCGTTAAC ACATCTGTGT CCACCATGGC ATCTACAGTC 
ACTCCAATGT ATGCAGGGGA TCTTCGGACA AAGTTGCCTG TGGATGACAG AACAAGTGCC 
ACTGACCATA AATTTCCTTC TTTGATGGAA AATTCAGATT CTCCGGTTAG AAGCATTCTG 
AAGTCCCAAG TCTGGCAGCC TTTGACTGAG GGCAGTGGAA ACAGAGGAAT GTTGAGAGAG 
CTGGGCGAGA CAGAAGGGAA GAGAGTCTTG GCAGGTGGAG ATGGTGGTAA AAAGTACGGG 
TCCTTTGAGG AAGTGGAGCC ACCCTGTCCT ACCCTGAACA GAGTCTGGGA AGGAGACAGC 
CATAAGGAAA CCAAATATGC TGTTCCAAGA AAGGGTAGCC TGGAACTAGC TCACCCTCCC 
GTTGCCCACC TTGGCGATGA ACTGAAGGAG TTCTCCGTGG GTAAAAGCAT TGCACAGGGG 
AGCCCAGACT GGACAGATAG GCAGCCCTTT GAAGAGAAGG TTGACCTGGA AAATGTCACG 
AGAAGGAGGT TTTCGCTAAG AGAGTTTGGG GAGCCTGCTT CTGAGCAGAC GGGAACAGCA 
GCCGGGAAAA CCGTCACCCA GACTGCAGCC CCCATGTCCT GGAAGCAGCA TGATTCTGTA 
GAACCAGTAC AGGAGAAGTA CCCCAGGAAT CCATGTGCCA TGTTTGCTGC CGGAGAGATC 
AAAGTGCCAG CGGTGGAGGG CATTCTGGAT TCAGCCAGCA AAACCATGTC AATTAAAGAA 
AGATTAGCGC TGTTGAAGAA GAGTGGTGAA GAAGATTGGA AGAACAGACT GAGCAGAAAG 
CAGGAGTACG GCAAGGCGTC TGTCTCCAGC AGCCTGCACA TACAAGAAGC GGAACAGTCC 
CTCAAGAAGA AGGAAGAAGG AGGATTTGCA GATGATAGTG CCATTTCTAA TCTGCTTTGG 
GAACCTGTAT ATGCTTCTAC TTATTCTCCT GCTATACCTG CTGCCCATAA ACACCTGTCT 
TTTGTATCTA TTAATCAGCG GGTCACAGAA AGTCGAGAGA GCCAGATGAC TATCGAAGAG 
AGGAAGCATC TCATCACTGT GAGAGAAGAA GCTTGGAAGA CAAAAGGCAA AGGAGCGGCC 
AACGACTCCA CCCAGTTTAC GGTGGCTGGC AGAATGGTGA AGAAAGGTTT GGCATCACCC 
ACCGCCATAA CTCCAGTAGC GTCTCCTATT TGCAGTAAAA CGAGGGGCAC CACACCTGTT 
TCTAAACCTT TGGAAGATAT TGAAGCCAGA CCAGACATGC AGTTGGAATC GGACCTGAAG 
TTGGACAGGC TGGAAACCTT TCTAAGAAGG CTGAATAACA AAGTTGGTGG GATGCAGGAG 
ACTGTACTCA CTGTCACCGG TAAATCTGTT AAAGAGGTGA TGAAGTTGGA TGATGACGAG 
ACCTTTGCCA GATTTTACCG CAGCATGGAT TATACTGTGC CACGAAGCCC CGTGGAGCTA 
GACGAAGATT TCGATGTCAT TTTCGATCCT TATGCCCCTA AGTTGACCTC CTCTGTGGCC 
GAGCACAAGC GCGCAGTTAG GCCCAAGCGC CGGGTTCAGG CTTCCAGAAA CCCCTTGAAA 
ATGCTGGCAG CCAGAGAAGA TCTCCTTCAG GAGTACACGG AGCAGAGACT AAACATTGCC 
TTCATGGAGT CAAAGCGGAT GAAAGTAGAA AAGATGTCCT CCAACTCCAA CTTCTCAGAA 
GTCACTCTGG CAGGCTTAGC CAGTAAAGAA AACTTCAGCA ACATCAGCCT GCGGAGCGTC 
AACCTGACGG AACAGAATTC CAACAATAGC GCGGTACCCT ATAAGAAGCT GATGTTGTTA 
CAGATTAAAG GAAGAAGACA CGTGCAGACA AGACTGGTCG AACCTCGAGC TTCATCCCTC 
AACAGTGGGG ACTGCTTCCT CCTGCTCTCC CCTCACTACT GCTTCGTGTG GGTAGGAGAG 
TTTGCAAATG TCATAGAAAA GGCAAAGGCC TCGGAACTTG CAAGTTTAAT TCAGACAAAG 
AGGGAACTTG GTTGTAGAGC TACATATGTC CAAACTATCG AAGAAGGAAT TAATACACAC 
ACTCATGCGG CCAAAGACTT CTGGAAGCTT CTGGGTGGCC AAACCAGTTA CCAGTCTGCC 
GGAGACCCAA AAGAAGATGA ACTTTATGAA ACAGCCATAA TTGAAACAAA CTGCATTTAC 
CGTCTGATGG ATGACAAACT GGTCCCTGAG GACGACTACT GGGGAAAGAT TCCCAAGTGC 
TCCCTTCTGC AGTCAAAAGA GGTACTGGTG TTTGATTTTG GTAGTGAAGT GTACGTGTGG 
CATGGAAAAG AAGTCACATT AGCGCAACGG AAAATAGCTT TTCAACTGGC AAAGCACTTA 
TGGAACGGAA CCTTTGACTA CGAGAACTGT GACATCAACC CGTTGGATCC TGGAGAATGC 
AATCCGCTTA TTCCCAGAAA AGGACAAGGG CGACCGGATT GGGCAATTTT CGGGAGACTT 
ACAGAACACA ATGAGACGAT TTTGTTCAAA GAGAAATTTC TGGATTGGAC GGAACTGAAG 
AGGCCTAATG AGAAGAGTGC CGGCGAACTC ACCCAGCAAA AGGAAGATCC CAGGGCAGAA 
GCCAAGGCAT ATGACGTGAC ACGGATGGTG CCGGCACCCC AGGCCACAAC CGGCACTGTG 
CTGGATGGGG TGAATGTTGG TCGTGGCTAC GGCTTGGTGG ATGGGGATGA CAGGAGGCAG 
TTTGAGATCG CCAGTGTGTC TGTGGATGTG TGGCACATCC TAGAATTTGA CTACAGCAGG 
CTACCCAAAC AGAGCATCGG GCAGTTCCAC GAGGGAGACG CCTATGTGGT CAAGTGGAAG 
TACATGGTGA GCACCGCAGT GGGAAGTCGA CAAAAGGGAG AGCACCCAGT ACGGGTAGCT 
GGCAAGGAGA AGTGTGTCTA CTTCTTCTGG CAAGGCCGGC AGTCGACCGT GAGTGAGAAG 
GGCACATCAG CTCTGATGAC AGTGGAGCTG GATGAGGAGA GGGGTGCCCA GGTCCAGGTT 
CTGCAGGGAA AGGAGCCCCC CTGCTTTCTG CAGTGTTTCC AGGGGGGAAT GGTGGTACAC 
TCTGGGAGAC GGGAAGAGGA GGAAGAAAAT GCACAAAGTG AGTGGAGGCT GTACTGTGTC 
CGAGGAGAAG TGCCCATGGA AGGAAATTTG CTGGAAGTGG CCTGTCACTG TAGTAGCCTG 
AGGTCCAGGA CTTCCATGGT TGTCCTCAAC ATAAACAAAG CCCTCATTTA CTTATGGCAC 
GGATGCAAAG CCCAAGCCCA CACGAAGGAG GTTGGAAGGA CTGCAGCAAA TAAAATCAAA 
GAACAATGTC CCCTGGAAGC AGGACTGCAT AGTAGCAGCA AAGTGACAAT ACACGAGTGT 
GACGAAGGAT CCGAGCCGCT GGGATTCTGG GATGCTTTAG GAAGGAGAGA TAGAAAAGCG 
TACGACTGTA TGCTTCAAGA TCCTGGAAAT TTTAACTTCA CACCTCGCCT ATTCATCCTC 
AGCAGTTCCT CCGGTGACTT CTTAGCCACA GAGTTCATAT ATCCCGCCCG AGACCCCTCT 
GTGGTCAACT CCATGCCTTT CCTGCAGGAA GATCTTTACA GCGCGCCACA GCCAGCACTT 
TTCCTTGTTG ACAATCACCA CGAGGTATAC CTCTGGCAAG GCTGGTGGCC CATAGAGAAC 
AAGATCACTG GATCGGCCCG CATTCGCTGG GCCTCCGACC GGAAGAGCGC CATGGAAACC 
GTGCTCCAGT ACTGCAAAGG AAAAAATATC AAGAGGCCGC CCCCCAAGTC TTACCTTATC 
CATGCTGGTC TGGAGCCCTT GACATTCACC AACATGTTCC CCAGCTGGGA GCACAGAGAG 
GACATTGCTG AAATCACAGA AATGGATACC GAAGTTTCAA ATCAGATCAC CCTTGTGGAA 
GATGTCTTAG CCAAGCTCTG TAAAACCATC TATCCCCTGG CTGATCTGCT AGCCAGGCCG 
CTACCCGAGG GAGTTGATCC TCTGAAGCTT GAGATTTATC TCACCGATGA AGACTTTGAG 
TTTGCACTAG ACATGACAAG AGACGAATAC AATGCTCTGC CCGCCTGGAA GCAGGTGAAC 
CTGAAGAAAG CAAAGGGTCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617047.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617104.1 Canis lupus familiaris supervillin (SVIL), transcript variant X3, mRNA.

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CloneID	OCe02721
Clone ID Related Accession (Same CDS sequence)	XM_005617047.2 , XM_005617047.3
Accession Version	XM_005617047.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	supervillin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726048.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617047.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACTG AACCTATCCC TGCTTCGTGC TCTGTGGCCA CCCACCCTGT AATGGCAAGA 
CTTCCTAGTC CCACTGTAGC TAAGAGCACT GTGCAGCCTG CCAGGTTGCA GGCATCTGCT 
CACCACAAAG CCTTAGCCAG AGACCAGGCA AATGAGGGCA AAGATGCTGC TGAACAAGGT 
GAACCTGACT CCTCCACTCT AAGCTTAGCA GAGAAGTTGG CCTTGTTTAA CAAATTATCC 
CAGCCAGTCT CAAAAGCTAT TTCTACCCGA AACAGAATAG ACATGAGACA GAGGAGAATG 
AATGCTCGCT ATCAGACTCA GCCAGTCACG CTTGGAGAGG TGGAACAGGT GCAAAGTGGA 
AAGCTCATTC CTTTCTCACC TACCGTTAAC ACATCTGTGT CCACCATGGC ATCTACAGTC 
ACTCCAATGT ATGCAGGGGA TCTTCGGACA AAGTTGCCTG TGGATGACAG AACAAGTGCC 
ACTGACCATA AATTTCCTTC TTTGATGGAA AATTCAGATT CTCCGGTTAG AAGCATTCTG 
AAGTCCCAAG TCTGGCAGCC TTTGACTGAG GGCAGTGGAA ACAGAGGAAT GTTGAGAGAG 
CTGGGCGAGA CAGAAGGGAA GAGAGTCTTG GCAGGTGGAG ATGGTGGTAA AAAGTACGGG 
TCCTTTGAGG AAGTGGAGCC ACCCTGTCCT ACCCTGAACA GAGTCTGGGA AGGAGACAGC 
CATAAGGAAA CCAAATATGC TGTTCCAAGA AAGGGTAGCC TGGAACTAGC TCACCCTCCC 
GTTGCCCACC TTGGCGATGA ACTGAAGGAG TTCTCCGTGG GTAAAAGCAT TGCACAGGGG 
AGCCCAGACT GGACAGATAG GCAGCCCTTT GAAGAGAAGG TTGACCTGGA AAATGTCACG 
AGAAGGAGGT TTTCGCTAAG AGCAGAGTTT GGGGAGCCTG CTTCTGAGCA GACGGGAACA 
GCAGCCGGGA AAACCGTCAC CCAGACTGCA GCCCCCATGT CCTGGAAGCA GCATGATTCT 
GTAGAACCAG TACAGGAGAA GTACCCCAGG AATCCATGTG CCATGTTTGC TGCCGGAGAG 
ATCAAAGTGC CAGCGGTGGA GGGCATTCTG GATTCAGCCA GCAAAACCAT GTCAATTAAA 
GAAAGATTAG CGCTGTTGAA GAAGAGTGGT GAAGAAGATT GGAAGAACAG ACTGAGCAGA 
AAGCAGGAGT ACGGCAAGGC GTCTGTCTCC AGCAGCCTGC ACATACAAGA AGCGGAACAG 
TCCCTCAAGA AGAAGGAAGA AGGAGGATTT GCAGATGATA GTGCCATTTC TAATCTGCTT 
TGGCGGGTCA CAGAAAGTCG AGAGAGCCAG ATGACTATCG AAGAGAGGAA GCATCTCATC 
ACTGTGAGAG AAGAAGCTTG GAAGACAAAA GGCAAAGGAG CGGCCAACGA CTCCACCCAG 
TTTACGGTGG CTGGCAGAAT GGTGAAGAAA GGTTTGGCAT CACCCACCGC CATAACTCCA 
GTAGCGTCTC CTATTTGCAG TAAAACGAGG GGCACCACAC CTGTTTCTAA ACCTTTGGAA 
GATATTGAAG CCAGACCAGA CATGCAGTTG GAATCGGACC TGAAGTTGGA CAGGCTGGAA 
ACCTTTCTAA GAAGGCTGAA TAACAAAGTT GGTGGGATGC AGGAGACTGT ACTCACTGTC 
ACCGGTAAAT CTGTTAAAGA GGTGATGAAG TTGGATGATG ACGAGACCTT TGCCAGATTT 
TACCGCAGCA TGGATTATAC TGTGCCACGA AGCCCCGTGG AGCTAGACGA AGATTTCGAT 
GTCATTTTCG ATCCTTATGC CCCTAAGTTG ACCTCCTCTG TGGCCGAGCA CAAGCGCGCA 
GTTAGGCCCA AGCGCCGGGT TCAGGCTTCC AGAAACCCCT TGAAAATGCT GGCAGCCAGA 
GAAGATCTCC TTCAGGAGTA CACGGAGCAG AGACTAAACA TTGCCTTCAT GGAGTCAAAG 
CGGATGAAAG TAGAAAAGAT GTCCTCCAAC TCCAACTTCT CAGAAGTCAC TCTGGCAGGC 
TTAGCCAGTA AAGAAAACTT CAGCAACATC AGCCTGCGGA GCGTCAACCT GACGGAACAG 
AATTCCAACA ATAGCGCGGT ACCCTATAAG AAGCTGATGT TGTTACAGAT TAAAGGAAGA 
AGACACGTGC AGACAAGACT GGTCGAACCT CGAGCTTCAT CCCTCAACAG TGGGGACTGC 
TTCCTCCTGC TCTCCCCTCA CTACTGCTTC GTGTGGGTAG GAGAGTTTGC AAATGTCATA 
GAAAAGGCAA AGGCCTCGGA ACTTGCAAGT TTAATTCAGA CAAAGAGGGA ACTTGGTTGT 
AGAGCTACAT ATGTCCAAAC TATCGAAGAA GGAATTAATA CACACACTCA TGCGGCCAAA 
GACTTCTGGA AGCTTCTGGG TGGCCAAACC AGTTACCAGT CTGCCGGAGA CCCAAAAGAA 
GATGAACTTT ATGAAACAGC CATAATTGAA ACAAACTGCA TTTACCGTCT GATGGATGAC 
AAACTGGTCC CTGAGGACGA CTACTGGGGA AAGATTCCCA AGTGCTCCCT TCTGCAGTCA 
AAAGAGGTAC TGGTGTTTGA TTTTGGTAGT GAAGTGTACG TGTGGCATGG AAAAGAAGTC 
ACATTAGCGC AACGGAAAAT AGCTTTTCAA CTGGCAAAGC ACTTATGGAA CGGAACCTTT 
GACTACGAGA ACTGTGACAT CAACCCGTTG GATCCTGGAG AATGCAATCC GCTTATTCCC 
AGAAAAGGAC AAGGGCGACC GGATTGGGCA ATTTTCGGGA GACTTACAGA ACACAATGAG 
ACGATTTTGT TCAAAGAGAA ATTTCTGGAT TGGACGGAAC TGAAGAGGCC TAATGAGAAG 
AGTGCCGGCG AACTCACCCA GCAAAAGGAA GATCCCAGGG CAGAAGCCAA GGCATATGAC 
GTGACACGGA TGGTGCCGGC ACCCCAGGCC ACAACCGGCA CTGTGCTGGA TGGGGTGAAT 
GTTGGTCGTG GCTACGGCTT GGTGGATGGG GATGACAGGA GGCAGTTTGA GATCGCCAGT 
GTGTCTGTGG ATGTGTGGCA CATCCTAGAA TTTGACTACA GCAGGCTACC CAAACAGAGC 
ATCGGGCAGT TCCACGAGGG AGACGCCTAT GTGGTCAAGT GGAAGTACAT GGTGAGCACC 
GCAGTGGGAA GTCGACAAAA GGGAGAGCAC CCAGTACGGG TAGCTGGCAA GGAGAAGTGT 
GTCTACTTCT TCTGGCAAGG CCGGCAGTCG ACCGTGAGTG AGAAGGGCAC ATCAGCTCTG 
ATGACAGTGG AGCTGGATGA GGAGAGGGGT GCCCAGGTCC AGGTTCTGCA GGGAAAGGAG 
CCCCCCTGCT TTCTGCAGTG TTTCCAGGGG GGAATGGTGG TACACTCTGG GAGACGGGAA 
GAGGAGGAAG AAAATGCACA AAGTGAGTGG AGGCTGTACT GTGTCCGAGG AGAAGTGCCC 
ATGGAAGGAA ATTTGCTGGA AGTGGCCTGT CACTGTAGTA GCCTGAGGTC CAGGACTTCC 
ATGGTTGTCC TCAACATAAA CAAAGCCCTC ATTTACTTAT GGCACGGATG CAAAGCCCAA 
GCCCACACGA AGGAGGTTGG AAGGACTGCA GCAAATAAAA TCAAAGAACA ATGTCCCCTG 
GAAGCAGGAC TGCATAGTAG CAGCAAAGTG ACAATACACG AGTGTGACGA AGGATCCGAG 
CCGCTGGGAT TCTGGGATGC TTTAGGAAGG AGAGATAGAA AAGCGTACGA CTGTATGCTT 
CAAGATCCTG GAAATTTTAA CTTCACACCT CGCCTATTCA TCCTCAGCAG TTCCTCCGGT 
GACTTCTTAG CCACAGAGTT CATATATCCC GCCCGAGACC CCTCTGTGGT CAACTCCATG 
CCTTTCCTGC AGGAAGATCT TTACAGCGCG CCACAGCCAG CACTTTTCCT TGTTGACAAT 
CACCACGAGG TATACCTCTG GCAAGGCTGG TGGCCCATAG AGAACAAGAT CACTGGATCG 
GCCCGCATTC GCTGGGCCTC CGACCGGAAG AGCGCCATGG AAACCGTGCT CCAGTACTGC 
AAAGGAAAAA ATATCAAGAG GCCGCCCCCC AAGTCTTACC TTATCCATGC TGGTCTGGAG 
CCCTTGACAT TCACCAACAT GTTCCCCAGC TGGGAGCACA GAGAGGACAT TGCTGAAATC 
ACAGAAATGG ATACCGAAGT TTCAAATCAG ATCACCCTTG TGGAAGATGT CTTAGCCAAG 
CTCTGTAAAA CCATCTATCC CCTGGCTGAT CTGCTAGCCA GGCCGCTACC CGAGGGAGTT 
GATCCTCTGA AGCTTGAGAT TTATCTCACC GATGAAGACT TTGAGTTTGC ACTAGACATG 
ACAAGAGACG AATACAATGC TCTGCCCGCC TGGAAGCAGG TGAACCTGAA GAAAGCAAAG 
GGTCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617104.1
CDS	48..6959
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617047.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617047.2

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACTG AACCTATCCC TGCTTCGTGC TCTGTGGCCA CCCACCCTGT AATGGCAAGA 
CTTCCTAGTC CCACTGTAGC TAAGAGCACT GTGCAGCCTG CCAGGTTGCA GGCATCTGCT 
CACCACAAAG CCTTAGCCAG AGACCAGGCA AATGAGGGCA AAGATGCTGC TGAACAAGGT 
GAACCTGACT CCTCCACTCT AAGCTTAGCA GAGAAGTTGG CCTTGTTTAA CAAATTATCC 
CAGCCAGTCT CAAAAGCTAT TTCTACCCGA AACAGAATAG ACATGAGACA GAGGAGAATG 
AATGCTCGCT ATCAGACTCA GCCAGTCACG CTTGGAGAGG TGGAACAGGT GCAAAGTGGA 
AAGCTCATTC CTTTCTCACC TACCGTTAAC ACATCTGTGT CCACCATGGC ATCTACAGTC 
ACTCCAATGT ATGCAGGGGA TCTTCGGACA AAGTTGCCTG TGGATGACAG AACAAGTGCC 
ACTGACCATA AATTTCCTTC TTTGATGGAA AATTCAGATT CTCCGGTTAG AAGCATTCTG 
AAGTCCCAAG TCTGGCAGCC TTTGACTGAG GGCAGTGGAA ACAGAGGAAT GTTGAGAGAG 
CTGGGCGAGA CAGAAGGGAA GAGAGTCTTG GCAGGTGGAG ATGGTGGTAA AAAGTACGGG 
TCCTTTGAGG AAGTGGAGCC ACCCTGTCCT ACCCTGAACA GAGTCTGGGA AGGAGACAGC 
CATAAGGAAA CCAAATATGC TGTTCCAAGA AAGGGTAGCC TGGAACTAGC TCACCCTCCC 
GTTGCCCACC TTGGCGATGA ACTGAAGGAG TTCTCCGTGG GTAAAAGCAT TGCACAGGGG 
AGCCCAGACT GGACAGATAG GCAGCCCTTT GAAGAGAAGG TTGACCTGGA AAATGTCACG 
AGAAGGAGGT TTTCGCTAAG AGCAGAGTTT GGGGAGCCTG CTTCTGAGCA GACGGGAACA 
GCAGCCGGGA AAACCGTCAC CCAGACTGCA GCCCCCATGT CCTGGAAGCA GCATGATTCT 
GTAGAACCAG TACAGGAGAA GTACCCCAGG AATCCATGTG CCATGTTTGC TGCCGGAGAG 
ATCAAAGTGC CAGCGGTGGA GGGCATTCTG GATTCAGCCA GCAAAACCAT GTCAATTAAA 
GAAAGATTAG CGCTGTTGAA GAAGAGTGGT GAAGAAGATT GGAAGAACAG ACTGAGCAGA 
AAGCAGGAGT ACGGCAAGGC GTCTGTCTCC AGCAGCCTGC ACATACAAGA AGCGGAACAG 
TCCCTCAAGA AGAAGGAAGA AGGAGGATTT GCAGATGATA GTGCCATTTC TAATCTGCTT 
TGGCGGGTCA CAGAAAGTCG AGAGAGCCAG ATGACTATCG AAGAGAGGAA GCATCTCATC 
ACTGTGAGAG AAGAAGCTTG GAAGACAAAA GGCAAAGGAG CGGCCAACGA CTCCACCCAG 
TTTACGGTGG CTGGCAGAAT GGTGAAGAAA GGTTTGGCAT CACCCACCGC CATAACTCCA 
GTAGCGTCTC CTATTTGCAG TAAAACGAGG GGCACCACAC CTGTTTCTAA ACCTTTGGAA 
GATATTGAAG CCAGACCAGA CATGCAGTTG GAATCGGACC TGAAGTTGGA CAGGCTGGAA 
ACCTTTCTAA GAAGGCTGAA TAACAAAGTT GGTGGGATGC AGGAGACTGT ACTCACTGTC 
ACCGGTAAAT CTGTTAAAGA GGTGATGAAG TTGGATGATG ACGAGACCTT TGCCAGATTT 
TACCGCAGCA TGGATTATAC TGTGCCACGA AGCCCCGTGG AGCTAGACGA AGATTTCGAT 
GTCATTTTCG ATCCTTATGC CCCTAAGTTG ACCTCCTCTG TGGCCGAGCA CAAGCGCGCA 
GTTAGGCCCA AGCGCCGGGT TCAGGCTTCC AGAAACCCCT TGAAAATGCT GGCAGCCAGA 
GAAGATCTCC TTCAGGAGTA CACGGAGCAG AGACTAAACA TTGCCTTCAT GGAGTCAAAG 
CGGATGAAAG TAGAAAAGAT GTCCTCCAAC TCCAACTTCT CAGAAGTCAC TCTGGCAGGC 
TTAGCCAGTA AAGAAAACTT CAGCAACATC AGCCTGCGGA GCGTCAACCT GACGGAACAG 
AATTCCAACA ATAGCGCGGT ACCCTATAAG AAGCTGATGT TGTTACAGAT TAAAGGAAGA 
AGACACGTGC AGACAAGACT GGTCGAACCT CGAGCTTCAT CCCTCAACAG TGGGGACTGC 
TTCCTCCTGC TCTCCCCTCA CTACTGCTTC GTGTGGGTAG GAGAGTTTGC AAATGTCATA 
GAAAAGGCAA AGGCCTCGGA ACTTGCAAGT TTAATTCAGA CAAAGAGGGA ACTTGGTTGT 
AGAGCTACAT ATGTCCAAAC TATCGAAGAA GGAATTAATA CACACACTCA TGCGGCCAAA 
GACTTCTGGA AGCTTCTGGG TGGCCAAACC AGTTACCAGT CTGCCGGAGA CCCAAAAGAA 
GATGAACTTT ATGAAACAGC CATAATTGAA ACAAACTGCA TTTACCGTCT GATGGATGAC 
AAACTGGTCC CTGAGGACGA CTACTGGGGA AAGATTCCCA AGTGCTCCCT TCTGCAGTCA 
AAAGAGGTAC TGGTGTTTGA TTTTGGTAGT GAAGTGTACG TGTGGCATGG AAAAGAAGTC 
ACATTAGCGC AACGGAAAAT AGCTTTTCAA CTGGCAAAGC ACTTATGGAA CGGAACCTTT 
GACTACGAGA ACTGTGACAT CAACCCGTTG GATCCTGGAG AATGCAATCC GCTTATTCCC 
AGAAAAGGAC AAGGGCGACC GGATTGGGCA ATTTTCGGGA GACTTACAGA ACACAATGAG 
ACGATTTTGT TCAAAGAGAA ATTTCTGGAT TGGACGGAAC TGAAGAGGCC TAATGAGAAG 
AGTGCCGGCG AACTCACCCA GCAAAAGGAA GATCCCAGGG CAGAAGCCAA GGCATATGAC 
GTGACACGGA TGGTGCCGGC ACCCCAGGCC ACAACCGGCA CTGTGCTGGA TGGGGTGAAT 
GTTGGTCGTG GCTACGGCTT GGTGGATGGG GATGACAGGA GGCAGTTTGA GATCGCCAGT 
GTGTCTGTGG ATGTGTGGCA CATCCTAGAA TTTGACTACA GCAGGCTACC CAAACAGAGC 
ATCGGGCAGT TCCACGAGGG AGACGCCTAT GTGGTCAAGT GGAAGTACAT GGTGAGCACC 
GCAGTGGGAA GTCGACAAAA GGGAGAGCAC CCAGTACGGG TAGCTGGCAA GGAGAAGTGT 
GTCTACTTCT TCTGGCAAGG CCGGCAGTCG ACCGTGAGTG AGAAGGGCAC ATCAGCTCTG 
ATGACAGTGG AGCTGGATGA GGAGAGGGGT GCCCAGGTCC AGGTTCTGCA GGGAAAGGAG 
CCCCCCTGCT TTCTGCAGTG TTTCCAGGGG GGAATGGTGG TACACTCTGG GAGACGGGAA 
GAGGAGGAAG AAAATGCACA AAGTGAGTGG AGGCTGTACT GTGTCCGAGG AGAAGTGCCC 
ATGGAAGGAA ATTTGCTGGA AGTGGCCTGT CACTGTAGTA GCCTGAGGTC CAGGACTTCC 
ATGGTTGTCC TCAACATAAA CAAAGCCCTC ATTTACTTAT GGCACGGATG CAAAGCCCAA 
GCCCACACGA AGGAGGTTGG AAGGACTGCA GCAAATAAAA TCAAAGAACA ATGTCCCCTG 
GAAGCAGGAC TGCATAGTAG CAGCAAAGTG ACAATACACG AGTGTGACGA AGGATCCGAG 
CCGCTGGGAT TCTGGGATGC TTTAGGAAGG AGAGATAGAA AAGCGTACGA CTGTATGCTT 
CAAGATCCTG GAAATTTTAA CTTCACACCT CGCCTATTCA TCCTCAGCAG TTCCTCCGGT 
GACTTCTTAG CCACAGAGTT CATATATCCC GCCCGAGACC CCTCTGTGGT CAACTCCATG 
CCTTTCCTGC AGGAAGATCT TTACAGCGCG CCACAGCCAG CACTTTTCCT TGTTGACAAT 
CACCACGAGG TATACCTCTG GCAAGGCTGG TGGCCCATAG AGAACAAGAT CACTGGATCG 
GCCCGCATTC GCTGGGCCTC CGACCGGAAG AGCGCCATGG AAACCGTGCT CCAGTACTGC 
AAAGGAAAAA ATATCAAGAG GCCGCCCCCC AAGTCTTACC TTATCCATGC TGGTCTGGAG 
CCCTTGACAT TCACCAACAT GTTCCCCAGC TGGGAGCACA GAGAGGACAT TGCTGAAATC 
ACAGAAATGG ATACCGAAGT TTCAAATCAG ATCACCCTTG TGGAAGATGT CTTAGCCAAG 
CTCTGTAAAA CCATCTATCC CCTGGCTGAT CTGCTAGCCA GGCCGCTACC CGAGGGAGTT 
GATCCTCTGA AGCTTGAGAT TTATCTCACC GATGAAGACT TTGAGTTTGC ACTAGACATG 
ACAAGAGACG AATACAATGC TCTGCCCGCC TGGAAGCAGG TGAACCTGAA GAAAGCAAAG 
GGTCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617047.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617104.1 Canis lupus familiaris supervillin (SVIL), transcript variant X3, mRNA.

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CloneID	OCe02721
Clone ID Related Accession (Same CDS sequence)	XM_005617047.2 , XM_005617047.3
Accession Version	XM_005617047.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617047.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACTG AACCTATCCC TGCTTCGTGC TCTGTGGCCA CCCACCCTGT AATGGCAAGA 
CTTCCTAGTC CCACTGTAGC TAAGAGCACT GTGCAGCCTG CCAGGTTGCA GGCATCTGCT 
CACCACAAAG CCTTAGCCAG AGACCAGGCA AATGAGGGCA AAGATGCTGC TGAACAAGGT 
GAACCTGACT CCTCCACTCT AAGCTTAGCA GAGAAGTTGG CCTTGTTTAA CAAATTATCC 
CAGCCAGTCT CAAAAGCTAT TTCTACCCGA AACAGAATAG ACATGAGACA GAGGAGAATG 
AATGCTCGCT ATCAGACTCA GCCAGTCACG CTTGGAGAGG TGGAACAGGT GCAAAGTGGA 
AAGCTCATTC CTTTCTCACC TACCGTTAAC ACATCTGTGT CCACCATGGC ATCTACAGTC 
ACTCCAATGT ATGCAGGGGA TCTTCGGACA AAGTTGCCTG TGGATGACAG AACAAGTGCC 
ACTGACCATA AATTTCCTTC TTTGATGGAA AATTCAGATT CTCCGGTTAG AAGCATTCTG 
AAGTCCCAAG TCTGGCAGCC TTTGACTGAG GGCAGTGGAA ACAGAGGAAT GTTGAGAGAG 
CTGGGCGAGA CAGAAGGGAA GAGAGTCTTG GCAGGTGGAG ATGGTGGTAA AAAGTACGGG 
TCCTTTGAGG AAGTGGAGCC ACCCTGTCCT ACCCTGAACA GAGTCTGGGA AGGAGACAGC 
CATAAGGAAA CCAAATATGC TGTTCCAAGA AAGGGTAGCC TGGAACTAGC TCACCCTCCC 
GTTGCCCACC TTGGCGATGA ACTGAAGGAG TTCTCCGTGG GTAAAAGCAT TGCACAGGGG 
AGCCCAGACT GGACAGATAG GCAGCCCTTT GAAGAGAAGG TTGACCTGGA AAATGTCACG 
AGAAGGAGGT TTTCGCTAAG AGCAGAGTTT GGGGAGCCTG CTTCTGAGCA GACGGGAACA 
GCAGCCGGGA AAACCGTCAC CCAGACTGCA GCCCCCATGT CCTGGAAGCA GCATGATTCT 
GTAGAACCAG TACAGGAGAA GTACCCCAGG AATCCATGTG CCATGTTTGC TGCCGGAGAG 
ATCAAAGTGC CAGCGGTGGA GGGCATTCTG GATTCAGCCA GCAAAACCAT GTCAATTAAA 
GAAAGATTAG CGCTGTTGAA GAAGAGTGGT GAAGAAGATT GGAAGAACAG ACTGAGCAGA 
AAGCAGGAGT ACGGCAAGGC GTCTGTCTCC AGCAGCCTGC ACATACAAGA AGCGGAACAG 
TCCCTCAAGA AGAAGGAAGA AGGAGGATTT GCAGATGATA GTGCCATTTC TAATCTGCTT 
TGGCGGGTCA CAGAAAGTCG AGAGAGCCAG ATGACTATCG AAGAGAGGAA GCATCTCATC 
ACTGTGAGAG AAGAAGCTTG GAAGACAAAA GGCAAAGGAG CGGCCAACGA CTCCACCCAG 
TTTACGGTGG CTGGCAGAAT GGTGAAGAAA GGTTTGGCAT CACCCACCGC CATAACTCCA 
GTAGCGTCTC CTATTTGCAG TAAAACGAGG GGCACCACAC CTGTTTCTAA ACCTTTGGAA 
GATATTGAAG CCAGACCAGA CATGCAGTTG GAATCGGACC TGAAGTTGGA CAGGCTGGAA 
ACCTTTCTAA GAAGGCTGAA TAACAAAGTT GGTGGGATGC AGGAGACTGT ACTCACTGTC 
ACCGGTAAAT CTGTTAAAGA GGTGATGAAG TTGGATGATG ACGAGACCTT TGCCAGATTT 
TACCGCAGCA TGGATTATAC TGTGCCACGA AGCCCCGTGG AGCTAGACGA AGATTTCGAT 
GTCATTTTCG ATCCTTATGC CCCTAAGTTG ACCTCCTCTG TGGCCGAGCA CAAGCGCGCA 
GTTAGGCCCA AGCGCCGGGT TCAGGCTTCC AGAAACCCCT TGAAAATGCT GGCAGCCAGA 
GAAGATCTCC TTCAGGAGTA CACGGAGCAG AGACTAAACA TTGCCTTCAT GGAGTCAAAG 
CGGATGAAAG TAGAAAAGAT GTCCTCCAAC TCCAACTTCT CAGAAGTCAC TCTGGCAGGC 
TTAGCCAGTA AAGAAAACTT CAGCAACATC AGCCTGCGGA GCGTCAACCT GACGGAACAG 
AATTCCAACA ATAGCGCGGT ACCCTATAAG AAGCTGATGT TGTTACAGAT TAAAGGAAGA 
AGACACGTGC AGACAAGACT GGTCGAACCT CGAGCTTCAT CCCTCAACAG TGGGGACTGC 
TTCCTCCTGC TCTCCCCTCA CTACTGCTTC GTGTGGGTAG GAGAGTTTGC AAATGTCATA 
GAAAAGGCAA AGGCCTCGGA ACTTGCAAGT TTAATTCAGA CAAAGAGGGA ACTTGGTTGT 
AGAGCTACAT ATGTCCAAAC TATCGAAGAA GGAATTAATA CACACACTCA TGCGGCCAAA 
GACTTCTGGA AGCTTCTGGG TGGCCAAACC AGTTACCAGT CTGCCGGAGA CCCAAAAGAA 
GATGAACTTT ATGAAACAGC CATAATTGAA ACAAACTGCA TTTACCGTCT GATGGATGAC 
AAACTGGTCC CTGAGGACGA CTACTGGGGA AAGATTCCCA AGTGCTCCCT TCTGCAGTCA 
AAAGAGGTAC TGGTGTTTGA TTTTGGTAGT GAAGTGTACG TGTGGCATGG AAAAGAAGTC 
ACATTAGCGC AACGGAAAAT AGCTTTTCAA CTGGCAAAGC ACTTATGGAA CGGAACCTTT 
GACTACGAGA ACTGTGACAT CAACCCGTTG GATCCTGGAG AATGCAATCC GCTTATTCCC 
AGAAAAGGAC AAGGGCGACC GGATTGGGCA ATTTTCGGGA GACTTACAGA ACACAATGAG 
ACGATTTTGT TCAAAGAGAA ATTTCTGGAT TGGACGGAAC TGAAGAGGCC TAATGAGAAG 
AGTGCCGGCG AACTCACCCA GCAAAAGGAA GATCCCAGGG CAGAAGCCAA GGCATATGAC 
GTGACACGGA TGGTGCCGGC ACCCCAGGCC ACAACCGGCA CTGTGCTGGA TGGGGTGAAT 
GTTGGTCGTG GCTACGGCTT GGTGGATGGG GATGACAGGA GGCAGTTTGA GATCGCCAGT 
GTGTCTGTGG ATGTGTGGCA CATCCTAGAA TTTGACTACA GCAGGCTACC CAAACAGAGC 
ATCGGGCAGT TCCACGAGGG AGACGCCTAT GTGGTCAAGT GGAAGTACAT GGTGAGCACC 
GCAGTGGGAA GTCGACAAAA GGGAGAGCAC CCAGTACGGG TAGCTGGCAA GGAGAAGTGT 
GTCTACTTCT TCTGGCAAGG CCGGCAGTCG ACCGTGAGTG AGAAGGGCAC ATCAGCTCTG 
ATGACAGTGG AGCTGGATGA GGAGAGGGGT GCCCAGGTCC AGGTTCTGCA GGGAAAGGAG 
CCCCCCTGCT TTCTGCAGTG TTTCCAGGGG GGAATGGTGG TACACTCTGG GAGACGGGAA 
GAGGAGGAAG AAAATGCACA AAGTGAGTGG AGGCTGTACT GTGTCCGAGG AGAAGTGCCC 
ATGGAAGGAA ATTTGCTGGA AGTGGCCTGT CACTGTAGTA GCCTGAGGTC CAGGACTTCC 
ATGGTTGTCC TCAACATAAA CAAAGCCCTC ATTTACTTAT GGCACGGATG CAAAGCCCAA 
GCCCACACGA AGGAGGTTGG AAGGACTGCA GCAAATAAAA TCAAAGAACA ATGTCCCCTG 
GAAGCAGGAC TGCATAGTAG CAGCAAAGTG ACAATACACG AGTGTGACGA AGGATCCGAG 
CCGCTGGGAT TCTGGGATGC TTTAGGAAGG AGAGATAGAA AAGCGTACGA CTGTATGCTT 
CAAGATCCTG GAAATTTTAA CTTCACACCT CGCCTATTCA TCCTCAGCAG TTCCTCCGGT 
GACTTCTTAG CCACAGAGTT CATATATCCC GCCCGAGACC CCTCTGTGGT CAACTCCATG 
CCTTTCCTGC AGGAAGATCT TTACAGCGCG CCACAGCCAG CACTTTTCCT TGTTGACAAT 
CACCACGAGG TATACCTCTG GCAAGGCTGG TGGCCCATAG AGAACAAGAT CACTGGATCG 
GCCCGCATTC GCTGGGCCTC CGACCGGAAG AGCGCCATGG AAACCGTGCT CCAGTACTGC 
AAAGGAAAAA ATATCAAGAG GCCGCCCCCC AAGTCTTACC TTATCCATGC TGGTCTGGAG 
CCCTTGACAT TCACCAACAT GTTCCCCAGC TGGGAGCACA GAGAGGACAT TGCTGAAATC 
ACAGAAATGG ATACCGAAGT TTCAAATCAG ATCACCCTTG TGGAAGATGT CTTAGCCAAG 
CTCTGTAAAA CCATCTATCC CCTGGCTGAT CTGCTAGCCA GGCCGCTACC CGAGGGAGTT 
GATCCTCTGA AGCTTGAGAT TTATCTCACC GATGAAGACT TTGAGTTTGC ACTAGACATG 
ACAAGAGACG AATACAATGC TCTGCCCGCC TGGAAGCAGG TGAACCTGAA GAAAGCAAAG 
GGTCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617104.1
CDS	65..6976
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617047.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617047.3

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACTG AACCTATCCC TGCTTCGTGC TCTGTGGCCA CCCACCCTGT AATGGCAAGA 
CTTCCTAGTC CCACTGTAGC TAAGAGCACT GTGCAGCCTG CCAGGTTGCA GGCATCTGCT 
CACCACAAAG CCTTAGCCAG AGACCAGGCA AATGAGGGCA AAGATGCTGC TGAACAAGGT 
GAACCTGACT CCTCCACTCT AAGCTTAGCA GAGAAGTTGG CCTTGTTTAA CAAATTATCC 
CAGCCAGTCT CAAAAGCTAT TTCTACCCGA AACAGAATAG ACATGAGACA GAGGAGAATG 
AATGCTCGCT ATCAGACTCA GCCAGTCACG CTTGGAGAGG TGGAACAGGT GCAAAGTGGA 
AAGCTCATTC CTTTCTCACC TACCGTTAAC ACATCTGTGT CCACCATGGC ATCTACAGTC 
ACTCCAATGT ATGCAGGGGA TCTTCGGACA AAGTTGCCTG TGGATGACAG AACAAGTGCC 
ACTGACCATA AATTTCCTTC TTTGATGGAA AATTCAGATT CTCCGGTTAG AAGCATTCTG 
AAGTCCCAAG TCTGGCAGCC TTTGACTGAG GGCAGTGGAA ACAGAGGAAT GTTGAGAGAG 
CTGGGCGAGA CAGAAGGGAA GAGAGTCTTG GCAGGTGGAG ATGGTGGTAA AAAGTACGGG 
TCCTTTGAGG AAGTGGAGCC ACCCTGTCCT ACCCTGAACA GAGTCTGGGA AGGAGACAGC 
CATAAGGAAA CCAAATATGC TGTTCCAAGA AAGGGTAGCC TGGAACTAGC TCACCCTCCC 
GTTGCCCACC TTGGCGATGA ACTGAAGGAG TTCTCCGTGG GTAAAAGCAT TGCACAGGGG 
AGCCCAGACT GGACAGATAG GCAGCCCTTT GAAGAGAAGG TTGACCTGGA AAATGTCACG 
AGAAGGAGGT TTTCGCTAAG AGCAGAGTTT GGGGAGCCTG CTTCTGAGCA GACGGGAACA 
GCAGCCGGGA AAACCGTCAC CCAGACTGCA GCCCCCATGT CCTGGAAGCA GCATGATTCT 
GTAGAACCAG TACAGGAGAA GTACCCCAGG AATCCATGTG CCATGTTTGC TGCCGGAGAG 
ATCAAAGTGC CAGCGGTGGA GGGCATTCTG GATTCAGCCA GCAAAACCAT GTCAATTAAA 
GAAAGATTAG CGCTGTTGAA GAAGAGTGGT GAAGAAGATT GGAAGAACAG ACTGAGCAGA 
AAGCAGGAGT ACGGCAAGGC GTCTGTCTCC AGCAGCCTGC ACATACAAGA AGCGGAACAG 
TCCCTCAAGA AGAAGGAAGA AGGAGGATTT GCAGATGATA GTGCCATTTC TAATCTGCTT 
TGGCGGGTCA CAGAAAGTCG AGAGAGCCAG ATGACTATCG AAGAGAGGAA GCATCTCATC 
ACTGTGAGAG AAGAAGCTTG GAAGACAAAA GGCAAAGGAG CGGCCAACGA CTCCACCCAG 
TTTACGGTGG CTGGCAGAAT GGTGAAGAAA GGTTTGGCAT CACCCACCGC CATAACTCCA 
GTAGCGTCTC CTATTTGCAG TAAAACGAGG GGCACCACAC CTGTTTCTAA ACCTTTGGAA 
GATATTGAAG CCAGACCAGA CATGCAGTTG GAATCGGACC TGAAGTTGGA CAGGCTGGAA 
ACCTTTCTAA GAAGGCTGAA TAACAAAGTT GGTGGGATGC AGGAGACTGT ACTCACTGTC 
ACCGGTAAAT CTGTTAAAGA GGTGATGAAG TTGGATGATG ACGAGACCTT TGCCAGATTT 
TACCGCAGCA TGGATTATAC TGTGCCACGA AGCCCCGTGG AGCTAGACGA AGATTTCGAT 
GTCATTTTCG ATCCTTATGC CCCTAAGTTG ACCTCCTCTG TGGCCGAGCA CAAGCGCGCA 
GTTAGGCCCA AGCGCCGGGT TCAGGCTTCC AGAAACCCCT TGAAAATGCT GGCAGCCAGA 
GAAGATCTCC TTCAGGAGTA CACGGAGCAG AGACTAAACA TTGCCTTCAT GGAGTCAAAG 
CGGATGAAAG TAGAAAAGAT GTCCTCCAAC TCCAACTTCT CAGAAGTCAC TCTGGCAGGC 
TTAGCCAGTA AAGAAAACTT CAGCAACATC AGCCTGCGGA GCGTCAACCT GACGGAACAG 
AATTCCAACA ATAGCGCGGT ACCCTATAAG AAGCTGATGT TGTTACAGAT TAAAGGAAGA 
AGACACGTGC AGACAAGACT GGTCGAACCT CGAGCTTCAT CCCTCAACAG TGGGGACTGC 
TTCCTCCTGC TCTCCCCTCA CTACTGCTTC GTGTGGGTAG GAGAGTTTGC AAATGTCATA 
GAAAAGGCAA AGGCCTCGGA ACTTGCAAGT TTAATTCAGA CAAAGAGGGA ACTTGGTTGT 
AGAGCTACAT ATGTCCAAAC TATCGAAGAA GGAATTAATA CACACACTCA TGCGGCCAAA 
GACTTCTGGA AGCTTCTGGG TGGCCAAACC AGTTACCAGT CTGCCGGAGA CCCAAAAGAA 
GATGAACTTT ATGAAACAGC CATAATTGAA ACAAACTGCA TTTACCGTCT GATGGATGAC 
AAACTGGTCC CTGAGGACGA CTACTGGGGA AAGATTCCCA AGTGCTCCCT TCTGCAGTCA 
AAAGAGGTAC TGGTGTTTGA TTTTGGTAGT GAAGTGTACG TGTGGCATGG AAAAGAAGTC 
ACATTAGCGC AACGGAAAAT AGCTTTTCAA CTGGCAAAGC ACTTATGGAA CGGAACCTTT 
GACTACGAGA ACTGTGACAT CAACCCGTTG GATCCTGGAG AATGCAATCC GCTTATTCCC 
AGAAAAGGAC AAGGGCGACC GGATTGGGCA ATTTTCGGGA GACTTACAGA ACACAATGAG 
ACGATTTTGT TCAAAGAGAA ATTTCTGGAT TGGACGGAAC TGAAGAGGCC TAATGAGAAG 
AGTGCCGGCG AACTCACCCA GCAAAAGGAA GATCCCAGGG CAGAAGCCAA GGCATATGAC 
GTGACACGGA TGGTGCCGGC ACCCCAGGCC ACAACCGGCA CTGTGCTGGA TGGGGTGAAT 
GTTGGTCGTG GCTACGGCTT GGTGGATGGG GATGACAGGA GGCAGTTTGA GATCGCCAGT 
GTGTCTGTGG ATGTGTGGCA CATCCTAGAA TTTGACTACA GCAGGCTACC CAAACAGAGC 
ATCGGGCAGT TCCACGAGGG AGACGCCTAT GTGGTCAAGT GGAAGTACAT GGTGAGCACC 
GCAGTGGGAA GTCGACAAAA GGGAGAGCAC CCAGTACGGG TAGCTGGCAA GGAGAAGTGT 
GTCTACTTCT TCTGGCAAGG CCGGCAGTCG ACCGTGAGTG AGAAGGGCAC ATCAGCTCTG 
ATGACAGTGG AGCTGGATGA GGAGAGGGGT GCCCAGGTCC AGGTTCTGCA GGGAAAGGAG 
CCCCCCTGCT TTCTGCAGTG TTTCCAGGGG GGAATGGTGG TACACTCTGG GAGACGGGAA 
GAGGAGGAAG AAAATGCACA AAGTGAGTGG AGGCTGTACT GTGTCCGAGG AGAAGTGCCC 
ATGGAAGGAA ATTTGCTGGA AGTGGCCTGT CACTGTAGTA GCCTGAGGTC CAGGACTTCC 
ATGGTTGTCC TCAACATAAA CAAAGCCCTC ATTTACTTAT GGCACGGATG CAAAGCCCAA 
GCCCACACGA AGGAGGTTGG AAGGACTGCA GCAAATAAAA TCAAAGAACA ATGTCCCCTG 
GAAGCAGGAC TGCATAGTAG CAGCAAAGTG ACAATACACG AGTGTGACGA AGGATCCGAG 
CCGCTGGGAT TCTGGGATGC TTTAGGAAGG AGAGATAGAA AAGCGTACGA CTGTATGCTT 
CAAGATCCTG GAAATTTTAA CTTCACACCT CGCCTATTCA TCCTCAGCAG TTCCTCCGGT 
GACTTCTTAG CCACAGAGTT CATATATCCC GCCCGAGACC CCTCTGTGGT CAACTCCATG 
CCTTTCCTGC AGGAAGATCT TTACAGCGCG CCACAGCCAG CACTTTTCCT TGTTGACAAT 
CACCACGAGG TATACCTCTG GCAAGGCTGG TGGCCCATAG AGAACAAGAT CACTGGATCG 
GCCCGCATTC GCTGGGCCTC CGACCGGAAG AGCGCCATGG AAACCGTGCT CCAGTACTGC 
AAAGGAAAAA ATATCAAGAG GCCGCCCCCC AAGTCTTACC TTATCCATGC TGGTCTGGAG 
CCCTTGACAT TCACCAACAT GTTCCCCAGC TGGGAGCACA GAGAGGACAT TGCTGAAATC 
ACAGAAATGG ATACCGAAGT TTCAAATCAG ATCACCCTTG TGGAAGATGT CTTAGCCAAG 
CTCTGTAAAA CCATCTATCC CCTGGCTGAT CTGCTAGCCA GGCCGCTACC CGAGGGAGTT 
GATCCTCTGA AGCTTGAGAT TTATCTCACC GATGAAGACT TTGAGTTTGC ACTAGACATG 
ACAAGAGACG AATACAATGC TCTGCCCGCC TGGAAGCAGG TGAACCTGAA GAAAGCAAAG 
GGTCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617048.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617105.1 Canis lupus familiaris supervillin (SVIL), transcript variant X5, mRNA.

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CloneID	OCe02722
Clone ID Related Accession (Same CDS sequence)	XM_005617048.3 , XM_005617048.2
Accession Version	XM_005617048.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617048.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACGT TGCAGGCATC TGCTCACCAC AAAGCCTTAG CCAGAGACCA GGCAAATGAG 
GGCAAAGATG CTGCTGAACA AGGTGAACCT GACTCCTCCA CTCTAAGCTT AGCAGAGAAG 
TTGGCCTTGT TTAACAAATT ATCCCAGCCA GTCTCAAAAG CTATTTCTAC CCGAAACAGA 
ATAGACATGA GACAGAGGAG AATGAATGCT CGCTATCAGA CTCAGCCAGT CACGCTTGGA 
GAGGTGGAAC AGGTGCAAAG TGGAAAGCTC ATTCCTTTCT CACCTACCGT TAACACATCT 
GTGTCCACCA TGGCATCTAC AGTCACTCCA ATGTATGCAG GGGATCTTCG GACAAAGTTG 
CCTGTGGATG ACAGAACAAG TGCCACTGAC CATAAATTTC CTTCTTTGAT GGAAAATTCA 
GATTCTCCGG TTAGAAGCAT TCTGAAGTCC CAAGTCTGGC AGCCTTTGAC TGAGGGCAGT 
GGAAACAGAG GAATGTTGAG AGAGCTGGGC GAGACAGAAG GGAAGAGAGT CTTGGCAGGT 
GGAGATGGTG GTAAAAAGTA CGGGTCCTTT GAGGAAGTGG AGCCACCCTG TCCTACCCTG 
AACAGAGTCT GGGAAGGAGA CAGCCATAAG GAAACCAAAT ATGCTGTTCC AAGAAAGGGT 
AGCCTGGAAC TAGCTCACCC TCCCGTTGCC CACCTTGGCG ATGAACTGAA GGAGTTCTCC 
GTGGGTAAAA GCATTGCACA GGGGAGCCCA GACTGGACAG ATAGGCAGCC CTTTGAAGAG 
AAGGTTGACC TGGAAAATGT CACGAGAAGG AGGTTTTCGC TAAGAGCAGA GTTTGGGGAG 
CCTGCTTCTG AGCAGACGGG AACAGCAGCC GGGAAAACCG TCACCCAGAC TGCAGCCCCC 
ATGTCCTGGA AGCAGCATGA TTCTGTAGAA CCAGTACAGG AGAAGTACCC CAGGAATCCA 
TGTGCCATGT TTGCTGCCGG AGAGATCAAA GTGCCAGCGG TGGAGGGCAT TCTGGATTCA 
GCCAGCAAAA CCATGTCAAT TAAAGAAAGA TTAGCGCTGT TGAAGAAGAG TGGTGAAGAA 
GATTGGAAGA ACAGACTGAG CAGAAAGCAG GAGTACGGCA AGGCGTCTGT CTCCAGCAGC 
CTGCACATAC AAGAAGCGGA ACAGTCCCTC AAGAAGAAGG AAGAAGGAGG ATTTGCAGAT 
GATAGTGCCA TTTCTAATCT GCTTTGGGAA CCTGTATATG CTTCTACTTA TTCTCCTGCT 
ATACCTGCTG CCCATAAACA CCTGTCTTTT GTATCTATTA ATCAGCGGGT CACAGAAAGT 
CGAGAGAGCC AGATGACTAT CGAAGAGAGG AAGCATCTCA TCACTGTGAG AGAAGAAGCT 
TGGAAGACAA AAGGCAAAGG AGCGGCCAAC GACTCCACCC AGTTTACGGT GGCTGGCAGA 
ATGGTGAAGA AAGGTTTGGC ATCACCCACC GCCATAACTC CAGTAGCGTC TCCTATTTGC 
AGTAAAACGA GGGGCACCAC ACCTGTTTCT AAACCTTTGG AAGATATTGA AGCCAGACCA 
GACATGCAGT TGGAATCGGA CCTGAAGTTG GACAGGCTGG AAACCTTTCT AAGAAGGCTG 
AATAACAAAG TTGGTGGGAT GCAGGAGACT GTACTCACTG TCACCGGTAA ATCTGTTAAA 
GAGGTGATGA AGTTGGATGA TGACGAGACC TTTGCCAGAT TTTACCGCAG CATGGATTAT 
ACTGTGCCAC GAAGCCCCGT GGAGCTAGAC GAAGATTTCG ATGTCATTTT CGATCCTTAT 
GCCCCTAAGT TGACCTCCTC TGTGGCCGAG CACAAGCGCG CAGTTAGGCC CAAGCGCCGG 
GTTCAGGCTT CCAGAAACCC CTTGAAAATG CTGGCAGCCA GAGAAGATCT CCTTCAGGAG 
TACACGGAGC AGAGACTAAA CATTGCCTTC ATGGAGTCAA AGCGGATGAA AGTAGAAAAG 
ATGTCCTCCA ACTCCAACTT CTCAGAAGTC ACTCTGGCAG GCTTAGCCAG TAAAGAAAAC 
TTCAGCAACA TCAGCCTGCG GAGCGTCAAC CTGACGGAAC AGAATTCCAA CAATAGCGCG 
GTACCCTATA AGAAGCTGAT GTTGTTACAG ATTAAAGGAA GAAGACACGT GCAGACAAGA 
CTGGTCGAAC CTCGAGCTTC ATCCCTCAAC AGTGGGGACT GCTTCCTCCT GCTCTCCCCT 
CACTACTGCT TCGTGTGGGT AGGAGAGTTT GCAAATGTCA TAGAAAAGGC AAAGGCCTCG 
GAACTTGCAA GTTTAATTCA GACAAAGAGG GAACTTGGTT GTAGAGCTAC ATATGTCCAA 
ACTATCGAAG AAGGAATTAA TACACACACT CATGCGGCCA AAGACTTCTG GAAGCTTCTG 
GGTGGCCAAA CCAGTTACCA GTCTGCCGGA GACCCAAAAG AAGATGAACT TTATGAAACA 
GCCATAATTG AAACAAACTG CATTTACCGT CTGATGGATG ACAAACTGGT CCCTGAGGAC 
GACTACTGGG GAAAGATTCC CAAGTGCTCC CTTCTGCAGT CAAAAGAGGT ACTGGTGTTT 
GATTTTGGTA GTGAAGTGTA CGTGTGGCAT GGAAAAGAAG TCACATTAGC GCAACGGAAA 
ATAGCTTTTC AACTGGCAAA GCACTTATGG AACGGAACCT TTGACTACGA GAACTGTGAC 
ATCAACCCGT TGGATCCTGG AGAATGCAAT CCGCTTATTC CCAGAAAAGG ACAAGGGCGA 
CCGGATTGGG CAATTTTCGG GAGACTTACA GAACACAATG AGACGATTTT GTTCAAAGAG 
AAATTTCTGG ATTGGACGGA ACTGAAGAGG CCTAATGAGA AGAGTGCCGG CGAACTCACC 
CAGCAAAAGG AAGATCCCAG GGCAGAAGCC AAGGCATATG ACGTGACACG GATGGTGCCG 
GCACCCCAGG CCACAACCGG CACTGTGCTG GATGGGGTGA ATGTTGGTCG TGGCTACGGC 
TTGGTGGATG GGGATGACAG GAGGCAGTTT GAGATCGCCA GTGTGTCTGT GGATGTGTGG 
CACATCCTAG AATTTGACTA CAGCAGGCTA CCCAAACAGA GCATCGGGCA GTTCCACGAG 
GGAGACGCCT ATGTGGTCAA GTGGAAGTAC ATGGTGAGCA CCGCAGTGGG AAGTCGACAA 
AAGGGAGAGC ACCCAGTACG GGTAGCTGGC AAGGAGAAGT GTGTCTACTT CTTCTGGCAA 
GGCCGGCAGT CGACCGTGAG TGAGAAGGGC ACATCAGCTC TGATGACAGT GGAGCTGGAT 
GAGGAGAGGG GTGCCCAGGT CCAGGTTCTG CAGGGAAAGG AGCCCCCCTG CTTTCTGCAG 
TGTTTCCAGG GGGGAATGGT GGTACACTCT GGGAGACGGG AAGAGGAGGA AGAAAATGCA 
CAAAGTGAGT GGAGGCTGTA CTGTGTCCGA GGAGAAGTGC CCATGGAAGG AAATTTGCTG 
GAAGTGGCCT GTCACTGTAG TAGCCTGAGG TCCAGGACTT CCATGGTTGT CCTCAACATA 
AACAAAGCCC TCATTTACTT ATGGCACGGA TGCAAAGCCC AAGCCCACAC GAAGGAGGTT 
GGAAGGACTG CAGCAAATAA AATCAAAGAA CAATGTCCCC TGGAAGCAGG ACTGCATAGT 
AGCAGCAAAG TGACAATACA CGAGTGTGAC GAAGGATCCG AGCCGCTGGG ATTCTGGGAT 
GCTTTAGGAA GGAGAGATAG AAAAGCGTAC GACTGTATGC TTCAAGATCC TGGAAATTTT 
AACTTCACAC CTCGCCTATT CATCCTCAGC AGTTCCTCCG GTGACTTCTT AGCCACAGAG 
TTCATATATC CCGCCCGAGA CCCCTCTGTG GTCAACTCCA TGCCTTTCCT GCAGGAAGAT 
CTTTACAGCG CGCCACAGCC AGCACTTTTC CTTGTTGACA ATCACCACGA GGTATACCTC 
TGGCAAGGCT GGTGGCCCAT AGAGAACAAG ATCACTGGAT CGGCCCGCAT TCGCTGGGCC 
TCCGACCGGA AGAGCGCCAT GGAAACCGTG CTCCAGTACT GCAAAGGAAA AAATATCAAG 
AGGCCGCCCC CCAAGTCTTA CCTTATCCAT GCTGGTCTGG AGCCCTTGAC ATTCACCAAC 
ATGTTCCCCA GCTGGGAGCA CAGAGAGGAC ATTGCTGAAA TCACAGAAAT GGATACCGAA 
GTTTCAAATC AGATCACCCT TGTGGAAGAT GTCTTAGCCA AGCTCTGTAA AACCATCTAT 
CCCCTGGCTG ATCTGCTAGC CAGGCCGCTA CCCGAGGGAG TTGATCCTCT GAAGCTTGAG 
ATTTATCTCA CCGATGAAGA CTTTGAGTTT GCACTAGACA TGACAAGAGA CGAATACAAT 
GCTCTGCCCG CCTGGAAGCA GGTGAACCTG AAGAAAGCAA AGGGTCTGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617105.1
CDS	65..6958
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617048.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617048.3

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACGT TGCAGGCATC TGCTCACCAC AAAGCCTTAG CCAGAGACCA GGCAAATGAG 
GGCAAAGATG CTGCTGAACA AGGTGAACCT GACTCCTCCA CTCTAAGCTT AGCAGAGAAG 
TTGGCCTTGT TTAACAAATT ATCCCAGCCA GTCTCAAAAG CTATTTCTAC CCGAAACAGA 
ATAGACATGA GACAGAGGAG AATGAATGCT CGCTATCAGA CTCAGCCAGT CACGCTTGGA 
GAGGTGGAAC AGGTGCAAAG TGGAAAGCTC ATTCCTTTCT CACCTACCGT TAACACATCT 
GTGTCCACCA TGGCATCTAC AGTCACTCCA ATGTATGCAG GGGATCTTCG GACAAAGTTG 
CCTGTGGATG ACAGAACAAG TGCCACTGAC CATAAATTTC CTTCTTTGAT GGAAAATTCA 
GATTCTCCGG TTAGAAGCAT TCTGAAGTCC CAAGTCTGGC AGCCTTTGAC TGAGGGCAGT 
GGAAACAGAG GAATGTTGAG AGAGCTGGGC GAGACAGAAG GGAAGAGAGT CTTGGCAGGT 
GGAGATGGTG GTAAAAAGTA CGGGTCCTTT GAGGAAGTGG AGCCACCCTG TCCTACCCTG 
AACAGAGTCT GGGAAGGAGA CAGCCATAAG GAAACCAAAT ATGCTGTTCC AAGAAAGGGT 
AGCCTGGAAC TAGCTCACCC TCCCGTTGCC CACCTTGGCG ATGAACTGAA GGAGTTCTCC 
GTGGGTAAAA GCATTGCACA GGGGAGCCCA GACTGGACAG ATAGGCAGCC CTTTGAAGAG 
AAGGTTGACC TGGAAAATGT CACGAGAAGG AGGTTTTCGC TAAGAGCAGA GTTTGGGGAG 
CCTGCTTCTG AGCAGACGGG AACAGCAGCC GGGAAAACCG TCACCCAGAC TGCAGCCCCC 
ATGTCCTGGA AGCAGCATGA TTCTGTAGAA CCAGTACAGG AGAAGTACCC CAGGAATCCA 
TGTGCCATGT TTGCTGCCGG AGAGATCAAA GTGCCAGCGG TGGAGGGCAT TCTGGATTCA 
GCCAGCAAAA CCATGTCAAT TAAAGAAAGA TTAGCGCTGT TGAAGAAGAG TGGTGAAGAA 
GATTGGAAGA ACAGACTGAG CAGAAAGCAG GAGTACGGCA AGGCGTCTGT CTCCAGCAGC 
CTGCACATAC AAGAAGCGGA ACAGTCCCTC AAGAAGAAGG AAGAAGGAGG ATTTGCAGAT 
GATAGTGCCA TTTCTAATCT GCTTTGGGAA CCTGTATATG CTTCTACTTA TTCTCCTGCT 
ATACCTGCTG CCCATAAACA CCTGTCTTTT GTATCTATTA ATCAGCGGGT CACAGAAAGT 
CGAGAGAGCC AGATGACTAT CGAAGAGAGG AAGCATCTCA TCACTGTGAG AGAAGAAGCT 
TGGAAGACAA AAGGCAAAGG AGCGGCCAAC GACTCCACCC AGTTTACGGT GGCTGGCAGA 
ATGGTGAAGA AAGGTTTGGC ATCACCCACC GCCATAACTC CAGTAGCGTC TCCTATTTGC 
AGTAAAACGA GGGGCACCAC ACCTGTTTCT AAACCTTTGG AAGATATTGA AGCCAGACCA 
GACATGCAGT TGGAATCGGA CCTGAAGTTG GACAGGCTGG AAACCTTTCT AAGAAGGCTG 
AATAACAAAG TTGGTGGGAT GCAGGAGACT GTACTCACTG TCACCGGTAA ATCTGTTAAA 
GAGGTGATGA AGTTGGATGA TGACGAGACC TTTGCCAGAT TTTACCGCAG CATGGATTAT 
ACTGTGCCAC GAAGCCCCGT GGAGCTAGAC GAAGATTTCG ATGTCATTTT CGATCCTTAT 
GCCCCTAAGT TGACCTCCTC TGTGGCCGAG CACAAGCGCG CAGTTAGGCC CAAGCGCCGG 
GTTCAGGCTT CCAGAAACCC CTTGAAAATG CTGGCAGCCA GAGAAGATCT CCTTCAGGAG 
TACACGGAGC AGAGACTAAA CATTGCCTTC ATGGAGTCAA AGCGGATGAA AGTAGAAAAG 
ATGTCCTCCA ACTCCAACTT CTCAGAAGTC ACTCTGGCAG GCTTAGCCAG TAAAGAAAAC 
TTCAGCAACA TCAGCCTGCG GAGCGTCAAC CTGACGGAAC AGAATTCCAA CAATAGCGCG 
GTACCCTATA AGAAGCTGAT GTTGTTACAG ATTAAAGGAA GAAGACACGT GCAGACAAGA 
CTGGTCGAAC CTCGAGCTTC ATCCCTCAAC AGTGGGGACT GCTTCCTCCT GCTCTCCCCT 
CACTACTGCT TCGTGTGGGT AGGAGAGTTT GCAAATGTCA TAGAAAAGGC AAAGGCCTCG 
GAACTTGCAA GTTTAATTCA GACAAAGAGG GAACTTGGTT GTAGAGCTAC ATATGTCCAA 
ACTATCGAAG AAGGAATTAA TACACACACT CATGCGGCCA AAGACTTCTG GAAGCTTCTG 
GGTGGCCAAA CCAGTTACCA GTCTGCCGGA GACCCAAAAG AAGATGAACT TTATGAAACA 
GCCATAATTG AAACAAACTG CATTTACCGT CTGATGGATG ACAAACTGGT CCCTGAGGAC 
GACTACTGGG GAAAGATTCC CAAGTGCTCC CTTCTGCAGT CAAAAGAGGT ACTGGTGTTT 
GATTTTGGTA GTGAAGTGTA CGTGTGGCAT GGAAAAGAAG TCACATTAGC GCAACGGAAA 
ATAGCTTTTC AACTGGCAAA GCACTTATGG AACGGAACCT TTGACTACGA GAACTGTGAC 
ATCAACCCGT TGGATCCTGG AGAATGCAAT CCGCTTATTC CCAGAAAAGG ACAAGGGCGA 
CCGGATTGGG CAATTTTCGG GAGACTTACA GAACACAATG AGACGATTTT GTTCAAAGAG 
AAATTTCTGG ATTGGACGGA ACTGAAGAGG CCTAATGAGA AGAGTGCCGG CGAACTCACC 
CAGCAAAAGG AAGATCCCAG GGCAGAAGCC AAGGCATATG ACGTGACACG GATGGTGCCG 
GCACCCCAGG CCACAACCGG CACTGTGCTG GATGGGGTGA ATGTTGGTCG TGGCTACGGC 
TTGGTGGATG GGGATGACAG GAGGCAGTTT GAGATCGCCA GTGTGTCTGT GGATGTGTGG 
CACATCCTAG AATTTGACTA CAGCAGGCTA CCCAAACAGA GCATCGGGCA GTTCCACGAG 
GGAGACGCCT ATGTGGTCAA GTGGAAGTAC ATGGTGAGCA CCGCAGTGGG AAGTCGACAA 
AAGGGAGAGC ACCCAGTACG GGTAGCTGGC AAGGAGAAGT GTGTCTACTT CTTCTGGCAA 
GGCCGGCAGT CGACCGTGAG TGAGAAGGGC ACATCAGCTC TGATGACAGT GGAGCTGGAT 
GAGGAGAGGG GTGCCCAGGT CCAGGTTCTG CAGGGAAAGG AGCCCCCCTG CTTTCTGCAG 
TGTTTCCAGG GGGGAATGGT GGTACACTCT GGGAGACGGG AAGAGGAGGA AGAAAATGCA 
CAAAGTGAGT GGAGGCTGTA CTGTGTCCGA GGAGAAGTGC CCATGGAAGG AAATTTGCTG 
GAAGTGGCCT GTCACTGTAG TAGCCTGAGG TCCAGGACTT CCATGGTTGT CCTCAACATA 
AACAAAGCCC TCATTTACTT ATGGCACGGA TGCAAAGCCC AAGCCCACAC GAAGGAGGTT 
GGAAGGACTG CAGCAAATAA AATCAAAGAA CAATGTCCCC TGGAAGCAGG ACTGCATAGT 
AGCAGCAAAG TGACAATACA CGAGTGTGAC GAAGGATCCG AGCCGCTGGG ATTCTGGGAT 
GCTTTAGGAA GGAGAGATAG AAAAGCGTAC GACTGTATGC TTCAAGATCC TGGAAATTTT 
AACTTCACAC CTCGCCTATT CATCCTCAGC AGTTCCTCCG GTGACTTCTT AGCCACAGAG 
TTCATATATC CCGCCCGAGA CCCCTCTGTG GTCAACTCCA TGCCTTTCCT GCAGGAAGAT 
CTTTACAGCG CGCCACAGCC AGCACTTTTC CTTGTTGACA ATCACCACGA GGTATACCTC 
TGGCAAGGCT GGTGGCCCAT AGAGAACAAG ATCACTGGAT CGGCCCGCAT TCGCTGGGCC 
TCCGACCGGA AGAGCGCCAT GGAAACCGTG CTCCAGTACT GCAAAGGAAA AAATATCAAG 
AGGCCGCCCC CCAAGTCTTA CCTTATCCAT GCTGGTCTGG AGCCCTTGAC ATTCACCAAC 
ATGTTCCCCA GCTGGGAGCA CAGAGAGGAC ATTGCTGAAA TCACAGAAAT GGATACCGAA 
GTTTCAAATC AGATCACCCT TGTGGAAGAT GTCTTAGCCA AGCTCTGTAA AACCATCTAT 
CCCCTGGCTG ATCTGCTAGC CAGGCCGCTA CCCGAGGGAG TTGATCCTCT GAAGCTTGAG 
ATTTATCTCA CCGATGAAGA CTTTGAGTTT GCACTAGACA TGACAAGAGA CGAATACAAT 
GCTCTGCCCG CCTGGAAGCA GGTGAACCTG AAGAAAGCAA AGGGTCTGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617048.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617105.1 Canis lupus familiaris supervillin (SVIL), transcript variant X4, mRNA.

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CloneID	OCe02722
Clone ID Related Accession (Same CDS sequence)	XM_005617048.3 , XM_005617048.2
Accession Version	XM_005617048.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	supervillin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726048.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617048.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACGT TGCAGGCATC TGCTCACCAC AAAGCCTTAG CCAGAGACCA GGCAAATGAG 
GGCAAAGATG CTGCTGAACA AGGTGAACCT GACTCCTCCA CTCTAAGCTT AGCAGAGAAG 
TTGGCCTTGT TTAACAAATT ATCCCAGCCA GTCTCAAAAG CTATTTCTAC CCGAAACAGA 
ATAGACATGA GACAGAGGAG AATGAATGCT CGCTATCAGA CTCAGCCAGT CACGCTTGGA 
GAGGTGGAAC AGGTGCAAAG TGGAAAGCTC ATTCCTTTCT CACCTACCGT TAACACATCT 
GTGTCCACCA TGGCATCTAC AGTCACTCCA ATGTATGCAG GGGATCTTCG GACAAAGTTG 
CCTGTGGATG ACAGAACAAG TGCCACTGAC CATAAATTTC CTTCTTTGAT GGAAAATTCA 
GATTCTCCGG TTAGAAGCAT TCTGAAGTCC CAAGTCTGGC AGCCTTTGAC TGAGGGCAGT 
GGAAACAGAG GAATGTTGAG AGAGCTGGGC GAGACAGAAG GGAAGAGAGT CTTGGCAGGT 
GGAGATGGTG GTAAAAAGTA CGGGTCCTTT GAGGAAGTGG AGCCACCCTG TCCTACCCTG 
AACAGAGTCT GGGAAGGAGA CAGCCATAAG GAAACCAAAT ATGCTGTTCC AAGAAAGGGT 
AGCCTGGAAC TAGCTCACCC TCCCGTTGCC CACCTTGGCG ATGAACTGAA GGAGTTCTCC 
GTGGGTAAAA GCATTGCACA GGGGAGCCCA GACTGGACAG ATAGGCAGCC CTTTGAAGAG 
AAGGTTGACC TGGAAAATGT CACGAGAAGG AGGTTTTCGC TAAGAGCAGA GTTTGGGGAG 
CCTGCTTCTG AGCAGACGGG AACAGCAGCC GGGAAAACCG TCACCCAGAC TGCAGCCCCC 
ATGTCCTGGA AGCAGCATGA TTCTGTAGAA CCAGTACAGG AGAAGTACCC CAGGAATCCA 
TGTGCCATGT TTGCTGCCGG AGAGATCAAA GTGCCAGCGG TGGAGGGCAT TCTGGATTCA 
GCCAGCAAAA CCATGTCAAT TAAAGAAAGA TTAGCGCTGT TGAAGAAGAG TGGTGAAGAA 
GATTGGAAGA ACAGACTGAG CAGAAAGCAG GAGTACGGCA AGGCGTCTGT CTCCAGCAGC 
CTGCACATAC AAGAAGCGGA ACAGTCCCTC AAGAAGAAGG AAGAAGGAGG ATTTGCAGAT 
GATAGTGCCA TTTCTAATCT GCTTTGGGAA CCTGTATATG CTTCTACTTA TTCTCCTGCT 
ATACCTGCTG CCCATAAACA CCTGTCTTTT GTATCTATTA ATCAGCGGGT CACAGAAAGT 
CGAGAGAGCC AGATGACTAT CGAAGAGAGG AAGCATCTCA TCACTGTGAG AGAAGAAGCT 
TGGAAGACAA AAGGCAAAGG AGCGGCCAAC GACTCCACCC AGTTTACGGT GGCTGGCAGA 
ATGGTGAAGA AAGGTTTGGC ATCACCCACC GCCATAACTC CAGTAGCGTC TCCTATTTGC 
AGTAAAACGA GGGGCACCAC ACCTGTTTCT AAACCTTTGG AAGATATTGA AGCCAGACCA 
GACATGCAGT TGGAATCGGA CCTGAAGTTG GACAGGCTGG AAACCTTTCT AAGAAGGCTG 
AATAACAAAG TTGGTGGGAT GCAGGAGACT GTACTCACTG TCACCGGTAA ATCTGTTAAA 
GAGGTGATGA AGTTGGATGA TGACGAGACC TTTGCCAGAT TTTACCGCAG CATGGATTAT 
ACTGTGCCAC GAAGCCCCGT GGAGCTAGAC GAAGATTTCG ATGTCATTTT CGATCCTTAT 
GCCCCTAAGT TGACCTCCTC TGTGGCCGAG CACAAGCGCG CAGTTAGGCC CAAGCGCCGG 
GTTCAGGCTT CCAGAAACCC CTTGAAAATG CTGGCAGCCA GAGAAGATCT CCTTCAGGAG 
TACACGGAGC AGAGACTAAA CATTGCCTTC ATGGAGTCAA AGCGGATGAA AGTAGAAAAG 
ATGTCCTCCA ACTCCAACTT CTCAGAAGTC ACTCTGGCAG GCTTAGCCAG TAAAGAAAAC 
TTCAGCAACA TCAGCCTGCG GAGCGTCAAC CTGACGGAAC AGAATTCCAA CAATAGCGCG 
GTACCCTATA AGAAGCTGAT GTTGTTACAG ATTAAAGGAA GAAGACACGT GCAGACAAGA 
CTGGTCGAAC CTCGAGCTTC ATCCCTCAAC AGTGGGGACT GCTTCCTCCT GCTCTCCCCT 
CACTACTGCT TCGTGTGGGT AGGAGAGTTT GCAAATGTCA TAGAAAAGGC AAAGGCCTCG 
GAACTTGCAA GTTTAATTCA GACAAAGAGG GAACTTGGTT GTAGAGCTAC ATATGTCCAA 
ACTATCGAAG AAGGAATTAA TACACACACT CATGCGGCCA AAGACTTCTG GAAGCTTCTG 
GGTGGCCAAA CCAGTTACCA GTCTGCCGGA GACCCAAAAG AAGATGAACT TTATGAAACA 
GCCATAATTG AAACAAACTG CATTTACCGT CTGATGGATG ACAAACTGGT CCCTGAGGAC 
GACTACTGGG GAAAGATTCC CAAGTGCTCC CTTCTGCAGT CAAAAGAGGT ACTGGTGTTT 
GATTTTGGTA GTGAAGTGTA CGTGTGGCAT GGAAAAGAAG TCACATTAGC GCAACGGAAA 
ATAGCTTTTC AACTGGCAAA GCACTTATGG AACGGAACCT TTGACTACGA GAACTGTGAC 
ATCAACCCGT TGGATCCTGG AGAATGCAAT CCGCTTATTC CCAGAAAAGG ACAAGGGCGA 
CCGGATTGGG CAATTTTCGG GAGACTTACA GAACACAATG AGACGATTTT GTTCAAAGAG 
AAATTTCTGG ATTGGACGGA ACTGAAGAGG CCTAATGAGA AGAGTGCCGG CGAACTCACC 
CAGCAAAAGG AAGATCCCAG GGCAGAAGCC AAGGCATATG ACGTGACACG GATGGTGCCG 
GCACCCCAGG CCACAACCGG CACTGTGCTG GATGGGGTGA ATGTTGGTCG TGGCTACGGC 
TTGGTGGATG GGGATGACAG GAGGCAGTTT GAGATCGCCA GTGTGTCTGT GGATGTGTGG 
CACATCCTAG AATTTGACTA CAGCAGGCTA CCCAAACAGA GCATCGGGCA GTTCCACGAG 
GGAGACGCCT ATGTGGTCAA GTGGAAGTAC ATGGTGAGCA CCGCAGTGGG AAGTCGACAA 
AAGGGAGAGC ACCCAGTACG GGTAGCTGGC AAGGAGAAGT GTGTCTACTT CTTCTGGCAA 
GGCCGGCAGT CGACCGTGAG TGAGAAGGGC ACATCAGCTC TGATGACAGT GGAGCTGGAT 
GAGGAGAGGG GTGCCCAGGT CCAGGTTCTG CAGGGAAAGG AGCCCCCCTG CTTTCTGCAG 
TGTTTCCAGG GGGGAATGGT GGTACACTCT GGGAGACGGG AAGAGGAGGA AGAAAATGCA 
CAAAGTGAGT GGAGGCTGTA CTGTGTCCGA GGAGAAGTGC CCATGGAAGG AAATTTGCTG 
GAAGTGGCCT GTCACTGTAG TAGCCTGAGG TCCAGGACTT CCATGGTTGT CCTCAACATA 
AACAAAGCCC TCATTTACTT ATGGCACGGA TGCAAAGCCC AAGCCCACAC GAAGGAGGTT 
GGAAGGACTG CAGCAAATAA AATCAAAGAA CAATGTCCCC TGGAAGCAGG ACTGCATAGT 
AGCAGCAAAG TGACAATACA CGAGTGTGAC GAAGGATCCG AGCCGCTGGG ATTCTGGGAT 
GCTTTAGGAA GGAGAGATAG AAAAGCGTAC GACTGTATGC TTCAAGATCC TGGAAATTTT 
AACTTCACAC CTCGCCTATT CATCCTCAGC AGTTCCTCCG GTGACTTCTT AGCCACAGAG 
TTCATATATC CCGCCCGAGA CCCCTCTGTG GTCAACTCCA TGCCTTTCCT GCAGGAAGAT 
CTTTACAGCG CGCCACAGCC AGCACTTTTC CTTGTTGACA ATCACCACGA GGTATACCTC 
TGGCAAGGCT GGTGGCCCAT AGAGAACAAG ATCACTGGAT CGGCCCGCAT TCGCTGGGCC 
TCCGACCGGA AGAGCGCCAT GGAAACCGTG CTCCAGTACT GCAAAGGAAA AAATATCAAG 
AGGCCGCCCC CCAAGTCTTA CCTTATCCAT GCTGGTCTGG AGCCCTTGAC ATTCACCAAC 
ATGTTCCCCA GCTGGGAGCA CAGAGAGGAC ATTGCTGAAA TCACAGAAAT GGATACCGAA 
GTTTCAAATC AGATCACCCT TGTGGAAGAT GTCTTAGCCA AGCTCTGTAA AACCATCTAT 
CCCCTGGCTG ATCTGCTAGC CAGGCCGCTA CCCGAGGGAG TTGATCCTCT GAAGCTTGAG 
ATTTATCTCA CCGATGAAGA CTTTGAGTTT GCACTAGACA TGACAAGAGA CGAATACAAT 
GCTCTGCCCG CCTGGAAGCA GGTGAACCTG AAGAAAGCAA AGGGTCTGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617105.1
CDS	48..6941
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617048.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617048.2

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACGT TGCAGGCATC TGCTCACCAC AAAGCCTTAG CCAGAGACCA GGCAAATGAG 
GGCAAAGATG CTGCTGAACA AGGTGAACCT GACTCCTCCA CTCTAAGCTT AGCAGAGAAG 
TTGGCCTTGT TTAACAAATT ATCCCAGCCA GTCTCAAAAG CTATTTCTAC CCGAAACAGA 
ATAGACATGA GACAGAGGAG AATGAATGCT CGCTATCAGA CTCAGCCAGT CACGCTTGGA 
GAGGTGGAAC AGGTGCAAAG TGGAAAGCTC ATTCCTTTCT CACCTACCGT TAACACATCT 
GTGTCCACCA TGGCATCTAC AGTCACTCCA ATGTATGCAG GGGATCTTCG GACAAAGTTG 
CCTGTGGATG ACAGAACAAG TGCCACTGAC CATAAATTTC CTTCTTTGAT GGAAAATTCA 
GATTCTCCGG TTAGAAGCAT TCTGAAGTCC CAAGTCTGGC AGCCTTTGAC TGAGGGCAGT 
GGAAACAGAG GAATGTTGAG AGAGCTGGGC GAGACAGAAG GGAAGAGAGT CTTGGCAGGT 
GGAGATGGTG GTAAAAAGTA CGGGTCCTTT GAGGAAGTGG AGCCACCCTG TCCTACCCTG 
AACAGAGTCT GGGAAGGAGA CAGCCATAAG GAAACCAAAT ATGCTGTTCC AAGAAAGGGT 
AGCCTGGAAC TAGCTCACCC TCCCGTTGCC CACCTTGGCG ATGAACTGAA GGAGTTCTCC 
GTGGGTAAAA GCATTGCACA GGGGAGCCCA GACTGGACAG ATAGGCAGCC CTTTGAAGAG 
AAGGTTGACC TGGAAAATGT CACGAGAAGG AGGTTTTCGC TAAGAGCAGA GTTTGGGGAG 
CCTGCTTCTG AGCAGACGGG AACAGCAGCC GGGAAAACCG TCACCCAGAC TGCAGCCCCC 
ATGTCCTGGA AGCAGCATGA TTCTGTAGAA CCAGTACAGG AGAAGTACCC CAGGAATCCA 
TGTGCCATGT TTGCTGCCGG AGAGATCAAA GTGCCAGCGG TGGAGGGCAT TCTGGATTCA 
GCCAGCAAAA CCATGTCAAT TAAAGAAAGA TTAGCGCTGT TGAAGAAGAG TGGTGAAGAA 
GATTGGAAGA ACAGACTGAG CAGAAAGCAG GAGTACGGCA AGGCGTCTGT CTCCAGCAGC 
CTGCACATAC AAGAAGCGGA ACAGTCCCTC AAGAAGAAGG AAGAAGGAGG ATTTGCAGAT 
GATAGTGCCA TTTCTAATCT GCTTTGGGAA CCTGTATATG CTTCTACTTA TTCTCCTGCT 
ATACCTGCTG CCCATAAACA CCTGTCTTTT GTATCTATTA ATCAGCGGGT CACAGAAAGT 
CGAGAGAGCC AGATGACTAT CGAAGAGAGG AAGCATCTCA TCACTGTGAG AGAAGAAGCT 
TGGAAGACAA AAGGCAAAGG AGCGGCCAAC GACTCCACCC AGTTTACGGT GGCTGGCAGA 
ATGGTGAAGA AAGGTTTGGC ATCACCCACC GCCATAACTC CAGTAGCGTC TCCTATTTGC 
AGTAAAACGA GGGGCACCAC ACCTGTTTCT AAACCTTTGG AAGATATTGA AGCCAGACCA 
GACATGCAGT TGGAATCGGA CCTGAAGTTG GACAGGCTGG AAACCTTTCT AAGAAGGCTG 
AATAACAAAG TTGGTGGGAT GCAGGAGACT GTACTCACTG TCACCGGTAA ATCTGTTAAA 
GAGGTGATGA AGTTGGATGA TGACGAGACC TTTGCCAGAT TTTACCGCAG CATGGATTAT 
ACTGTGCCAC GAAGCCCCGT GGAGCTAGAC GAAGATTTCG ATGTCATTTT CGATCCTTAT 
GCCCCTAAGT TGACCTCCTC TGTGGCCGAG CACAAGCGCG CAGTTAGGCC CAAGCGCCGG 
GTTCAGGCTT CCAGAAACCC CTTGAAAATG CTGGCAGCCA GAGAAGATCT CCTTCAGGAG 
TACACGGAGC AGAGACTAAA CATTGCCTTC ATGGAGTCAA AGCGGATGAA AGTAGAAAAG 
ATGTCCTCCA ACTCCAACTT CTCAGAAGTC ACTCTGGCAG GCTTAGCCAG TAAAGAAAAC 
TTCAGCAACA TCAGCCTGCG GAGCGTCAAC CTGACGGAAC AGAATTCCAA CAATAGCGCG 
GTACCCTATA AGAAGCTGAT GTTGTTACAG ATTAAAGGAA GAAGACACGT GCAGACAAGA 
CTGGTCGAAC CTCGAGCTTC ATCCCTCAAC AGTGGGGACT GCTTCCTCCT GCTCTCCCCT 
CACTACTGCT TCGTGTGGGT AGGAGAGTTT GCAAATGTCA TAGAAAAGGC AAAGGCCTCG 
GAACTTGCAA GTTTAATTCA GACAAAGAGG GAACTTGGTT GTAGAGCTAC ATATGTCCAA 
ACTATCGAAG AAGGAATTAA TACACACACT CATGCGGCCA AAGACTTCTG GAAGCTTCTG 
GGTGGCCAAA CCAGTTACCA GTCTGCCGGA GACCCAAAAG AAGATGAACT TTATGAAACA 
GCCATAATTG AAACAAACTG CATTTACCGT CTGATGGATG ACAAACTGGT CCCTGAGGAC 
GACTACTGGG GAAAGATTCC CAAGTGCTCC CTTCTGCAGT CAAAAGAGGT ACTGGTGTTT 
GATTTTGGTA GTGAAGTGTA CGTGTGGCAT GGAAAAGAAG TCACATTAGC GCAACGGAAA 
ATAGCTTTTC AACTGGCAAA GCACTTATGG AACGGAACCT TTGACTACGA GAACTGTGAC 
ATCAACCCGT TGGATCCTGG AGAATGCAAT CCGCTTATTC CCAGAAAAGG ACAAGGGCGA 
CCGGATTGGG CAATTTTCGG GAGACTTACA GAACACAATG AGACGATTTT GTTCAAAGAG 
AAATTTCTGG ATTGGACGGA ACTGAAGAGG CCTAATGAGA AGAGTGCCGG CGAACTCACC 
CAGCAAAAGG AAGATCCCAG GGCAGAAGCC AAGGCATATG ACGTGACACG GATGGTGCCG 
GCACCCCAGG CCACAACCGG CACTGTGCTG GATGGGGTGA ATGTTGGTCG TGGCTACGGC 
TTGGTGGATG GGGATGACAG GAGGCAGTTT GAGATCGCCA GTGTGTCTGT GGATGTGTGG 
CACATCCTAG AATTTGACTA CAGCAGGCTA CCCAAACAGA GCATCGGGCA GTTCCACGAG 
GGAGACGCCT ATGTGGTCAA GTGGAAGTAC ATGGTGAGCA CCGCAGTGGG AAGTCGACAA 
AAGGGAGAGC ACCCAGTACG GGTAGCTGGC AAGGAGAAGT GTGTCTACTT CTTCTGGCAA 
GGCCGGCAGT CGACCGTGAG TGAGAAGGGC ACATCAGCTC TGATGACAGT GGAGCTGGAT 
GAGGAGAGGG GTGCCCAGGT CCAGGTTCTG CAGGGAAAGG AGCCCCCCTG CTTTCTGCAG 
TGTTTCCAGG GGGGAATGGT GGTACACTCT GGGAGACGGG AAGAGGAGGA AGAAAATGCA 
CAAAGTGAGT GGAGGCTGTA CTGTGTCCGA GGAGAAGTGC CCATGGAAGG AAATTTGCTG 
GAAGTGGCCT GTCACTGTAG TAGCCTGAGG TCCAGGACTT CCATGGTTGT CCTCAACATA 
AACAAAGCCC TCATTTACTT ATGGCACGGA TGCAAAGCCC AAGCCCACAC GAAGGAGGTT 
GGAAGGACTG CAGCAAATAA AATCAAAGAA CAATGTCCCC TGGAAGCAGG ACTGCATAGT 
AGCAGCAAAG TGACAATACA CGAGTGTGAC GAAGGATCCG AGCCGCTGGG ATTCTGGGAT 
GCTTTAGGAA GGAGAGATAG AAAAGCGTAC GACTGTATGC TTCAAGATCC TGGAAATTTT 
AACTTCACAC CTCGCCTATT CATCCTCAGC AGTTCCTCCG GTGACTTCTT AGCCACAGAG 
TTCATATATC CCGCCCGAGA CCCCTCTGTG GTCAACTCCA TGCCTTTCCT GCAGGAAGAT 
CTTTACAGCG CGCCACAGCC AGCACTTTTC CTTGTTGACA ATCACCACGA GGTATACCTC 
TGGCAAGGCT GGTGGCCCAT AGAGAACAAG ATCACTGGAT CGGCCCGCAT TCGCTGGGCC 
TCCGACCGGA AGAGCGCCAT GGAAACCGTG CTCCAGTACT GCAAAGGAAA AAATATCAAG 
AGGCCGCCCC CCAAGTCTTA CCTTATCCAT GCTGGTCTGG AGCCCTTGAC ATTCACCAAC 
ATGTTCCCCA GCTGGGAGCA CAGAGAGGAC ATTGCTGAAA TCACAGAAAT GGATACCGAA 
GTTTCAAATC AGATCACCCT TGTGGAAGAT GTCTTAGCCA AGCTCTGTAA AACCATCTAT 
CCCCTGGCTG ATCTGCTAGC CAGGCCGCTA CCCGAGGGAG TTGATCCTCT GAAGCTTGAG 
ATTTATCTCA CCGATGAAGA CTTTGAGTTT GCACTAGACA TGACAAGAGA CGAATACAAT 
GCTCTGCCCG CCTGGAAGCA GGTGAACCTG AAGAAAGCAA AGGGTCTGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617049.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617106.1 Canis lupus familiaris supervillin (SVIL), transcript variant X5, mRNA.

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CloneID	OCe02723
Clone ID Related Accession (Same CDS sequence)	XM_005617049.2 , XM_005617049.3
Accession Version	XM_005617049.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	supervillin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726048.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617049.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACTG AACCTATCCC TGCTTCGTGC TCTGTGGCCA CCCACCCTGT AATGGCAAGA 
CTTCCTAGTC CCACTGTAGC TAAGAGCACT GTGCAGCCTG CCAGGTTGCA GGCATCTGCT 
CACCACAAAG CCTTAGCCAG AGACCAGGCA AATGAGGGCA AAGATGCTGC TGAACAAGGT 
GAACCTGACT CCTCCACTCT AAGCTTAGCA GAGAAGTTGG CCTTGTTTAA CAAATTATCC 
CAGCCAGTCT CAAAAGCTAT TTCTACCCGA AACAGAATAG ACATGAGACA GAGGAGAATG 
AATGCTCGCT ATCAGACTCA GCCAGTCACG CTTGGAGAGG TGGAACAGGT GCAAAGTGGA 
AAGCTCATTC CTTTCTCACC TACCGTTAAC ACATCTGTGT CCACCATGGC ATCTACAGTC 
ACTCCAATGT ATGCAGGGGA TCTTCGGACA AAGTTGCCTG TGGATGACAG AACAAGTGCC 
ACTGACCATA AATTTCCTTC TTTGATGGAA AATTCAGATT CTCCGGTTAG AAGCATTCTG 
AAGTCCCAAG TCTGGCAGCC TTTGACTGAG GGCAGTGGAA ACAGAGGAAT GTTGAGAGAG 
CTGGGCGAGA CAGAAGGGAA GAGAGTCTTG GCAGGTGGAG ATGGTGGTAA AAAGTACGGG 
TCCTTTGAGG AAGTGGAGCC ACCCTGTCCT ACCCTGAACA GAGTCTGGGA AGGAGACAGC 
CATAAGGAAA CCAAATATGC TGTTCCAAGA AAGGGTAGCC TGGAACTAGC TCACCCTCCC 
GTTGCCCACC TTGGCGATGA ACTGAAGGAG TTCTCCGTGG GTAAAAGCAT TGCACAGGGG 
AGCCCAGACT GGACAGATAG GCAGCCCTTT GAAGAGAAGG TTGACCTGGA AAATGTCACG 
AGAAGGAGGT TTTCGCTAAG AGCAGAGTTT GGGGAGCCTG CTTCTGAGCA GACGGGAACA 
GCAGCCGGGA AAACCGTCAC CCAGACTGCA GCCCCCATGT CCTGGAAGCA GCATGATTCT 
GTAGAACCAG TACAGGAGAA GTACCCCAGG AATCCATGTG CCATGTTTGC TGCCGGAGAG 
ATCAAAGTGC CAGCGGTGGA GGGCATTCTG GATTCAGCCA GCAAAACCAT GTCAATTAAA 
GAAAGATTAG CGCTGTTGAA GAAGAGTGGT GAAGAAGATT GGAAGAACAG ACTGAGCAGA 
AAGCAGGAGT ACGGCAAGGC GTCTGTCTCC AGCAGCCTGC ACATACAAGA AGCGGAACAG 
TCCCTCAAGA AGAAGCGGGT CACAGAAAGT CGAGAGAGCC AGATGACTAT CGAAGAGAGG 
AAGCATCTCA TCACTGTGAG AGAAGAAGCT TGGAAGACAA AAGGCAAAGG AGCGGCCAAC 
GACTCCACCC AGTTTACGGT GGCTGGCAGA ATGGTGAAGA AAGGTTTGGC ATCACCCACC 
GCCATAACTC CAGTAGCGTC TCCTATTTGC AGTAAAACGA GGGGCACCAC ACCTGTTTCT 
AAACCTTTGG AAGATATTGA AGCCAGACCA GACATGCAGT TGGAATCGGA CCTGAAGTTG 
GACAGGCTGG AAACCTTTCT AAGAAGGCTG AATAACAAAG TTGGTGGGAT GCAGGAGACT 
GTACTCACTG TCACCGGTAA ATCTGTTAAA GAGGTGATGA AGTTGGATGA TGACGAGACC 
TTTGCCAGAT TTTACCGCAG CATGGATTAT ACTGTGCCAC GAAGCCCCGT GGAGCTAGAC 
GAAGATTTCG ATGTCATTTT CGATCCTTAT GCCCCTAAGT TGACCTCCTC TGTGGCCGAG 
CACAAGCGCG CAGTTAGGCC CAAGCGCCGG GTTCAGGCTT CCAGAAACCC CTTGAAAATG 
CTGGCAGCCA GAGAAGATCT CCTTCAGGAG TACACGGAGC AGAGACTAAA CATTGCCTTC 
ATGGAGTCAA AGCGGATGAA AGTAGAAAAG ATGTCCTCCA ACTCCAACTT CTCAGAAGTC 
ACTCTGGCAG GCTTAGCCAG TAAAGAAAAC TTCAGCAACA TCAGCCTGCG GAGCGTCAAC 
CTGACGGAAC AGAATTCCAA CAATAGCGCG GTACCCTATA AGAAGCTGAT GTTGTTACAG 
ATTAAAGGAA GAAGACACGT GCAGACAAGA CTGGTCGAAC CTCGAGCTTC ATCCCTCAAC 
AGTGGGGACT GCTTCCTCCT GCTCTCCCCT CACTACTGCT TCGTGTGGGT AGGAGAGTTT 
GCAAATGTCA TAGAAAAGGC AAAGGCCTCG GAACTTGCAA GTTTAATTCA GACAAAGAGG 
GAACTTGGTT GTAGAGCTAC ATATGTCCAA ACTATCGAAG AAGGAATTAA TACACACACT 
CATGCGGCCA AAGACTTCTG GAAGCTTCTG GGTGGCCAAA CCAGTTACCA GTCTGCCGGA 
GACCCAAAAG AAGATGAACT TTATGAAACA GCCATAATTG AAACAAACTG CATTTACCGT 
CTGATGGATG ACAAACTGGT CCCTGAGGAC GACTACTGGG GAAAGATTCC CAAGTGCTCC 
CTTCTGCAGT CAAAAGAGGT ACTGGTGTTT GATTTTGGTA GTGAAGTGTA CGTGTGGCAT 
GGAAAAGAAG TCACATTAGC GCAACGGAAA ATAGCTTTTC AACTGGCAAA GCACTTATGG 
AACGGAACCT TTGACTACGA GAACTGTGAC ATCAACCCGT TGGATCCTGG AGAATGCAAT 
CCGCTTATTC CCAGAAAAGG ACAAGGGCGA CCGGATTGGG CAATTTTCGG GAGACTTACA 
GAACACAATG AGACGATTTT GTTCAAAGAG AAATTTCTGG ATTGGACGGA ACTGAAGAGG 
CCTAATGAGA AGAGTGCCGG CGAACTCACC CAGCAAAAGG AAGATCCCAG GGCAGAAGCC 
AAGGCATATG ACGTGACACG GATGGTGCCG GCACCCCAGG CCACAACCGG CACTGTGCTG 
GATGGGGTGA ATGTTGGTCG TGGCTACGGC TTGGTGGATG GGGATGACAG GAGGCAGTTT 
GAGATCGCCA GTGTGTCTGT GGATGTGTGG CACATCCTAG AATTTGACTA CAGCAGGCTA 
CCCAAACAGA GCATCGGGCA GTTCCACGAG GGAGACGCCT ATGTGGTCAA GTGGAAGTAC 
ATGGTGAGCA CCGCAGTGGG AAGTCGACAA AAGGGAGAGC ACCCAGTACG GGTAGCTGGC 
AAGGAGAAGT GTGTCTACTT CTTCTGGCAA GGCCGGCAGT CGACCGTGAG TGAGAAGGGC 
ACATCAGCTC TGATGACAGT GGAGCTGGAT GAGGAGAGGG GTGCCCAGGT CCAGGTTCTG 
CAGGGAAAGG AGCCCCCCTG CTTTCTGCAG TGTTTCCAGG GGGGAATGGT GGTACACTCT 
GGGAGACGGG AAGAGGAGGA AGAAAATGCA CAAAGTGAGT GGAGGCTGTA CTGTGTCCGA 
GGAGAAGTGC CCATGGAAGG AAATTTGCTG GAAGTGGCCT GTCACTGTAG TAGCCTGAGG 
TCCAGGACTT CCATGGTTGT CCTCAACATA AACAAAGCCC TCATTTACTT ATGGCACGGA 
TGCAAAGCCC AAGCCCACAC GAAGGAGGTT GGAAGGACTG CAGCAAATAA AATCAAAGAA 
CAATGTCCCC TGGAAGCAGG ACTGCATAGT AGCAGCAAAG TGACAATACA CGAGTGTGAC 
GAAGGATCCG AGCCGCTGGG ATTCTGGGAT GCTTTAGGAA GGAGAGATAG AAAAGCGTAC 
GACTGTATGC TTCAAGATCC TGGAAATTTT AACTTCACAC CTCGCCTATT CATCCTCAGC 
AGTTCCTCCG GTGACTTCTT AGCCACAGAG TTCATATATC CCGCCCGAGA CCCCTCTGTG 
GTCAACTCCA TGCCTTTCCT GCAGGAAGAT CTTTACAGCG CGCCACAGCC AGCACTTTTC 
CTTGTTGACA ATCACCACGA GGTATACCTC TGGCAAGGCT GGTGGCCCAT AGAGAACAAG 
ATCACTGGAT CGGCCCGCAT TCGCTGGGCC TCCGACCGGA AGAGCGCCAT GGAAACCGTG 
CTCCAGTACT GCAAAGGAAA AAATATCAAG AGGCCGCCCC CCAAGTCTTA CCTTATCCAT 
GCTGGTCTGG AGCCCTTGAC ATTCACCAAC ATGTTCCCCA GCTGGGAGCA CAGAGAGGAC 
ATTGCTGAAA TCACAGAAAT GGATACCGAA GTTTCAAATC AGATCACCCT TGTGGAAGAT 
GTCTTAGCCA AGCTCTGTAA AACCATCTAT CCCCTGGCTG ATCTGCTAGC CAGGCCGCTA 
CCCGAGGGAG TTGATCCTCT GAAGCTTGAG ATTTATCTCA CCGATGAAGA CTTTGAGTTT 
GCACTAGACA TGACAAGAGA CGAATACAAT GCTCTGCCCG CCTGGAAGCA GGTGAACCTG 
AAGAAAGCAA AGGGTCTGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617106.1
CDS	47..6910
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617049.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617049.2

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACTG AACCTATCCC TGCTTCGTGC TCTGTGGCCA CCCACCCTGT AATGGCAAGA 
CTTCCTAGTC CCACTGTAGC TAAGAGCACT GTGCAGCCTG CCAGGTTGCA GGCATCTGCT 
CACCACAAAG CCTTAGCCAG AGACCAGGCA AATGAGGGCA AAGATGCTGC TGAACAAGGT 
GAACCTGACT CCTCCACTCT AAGCTTAGCA GAGAAGTTGG CCTTGTTTAA CAAATTATCC 
CAGCCAGTCT CAAAAGCTAT TTCTACCCGA AACAGAATAG ACATGAGACA GAGGAGAATG 
AATGCTCGCT ATCAGACTCA GCCAGTCACG CTTGGAGAGG TGGAACAGGT GCAAAGTGGA 
AAGCTCATTC CTTTCTCACC TACCGTTAAC ACATCTGTGT CCACCATGGC ATCTACAGTC 
ACTCCAATGT ATGCAGGGGA TCTTCGGACA AAGTTGCCTG TGGATGACAG AACAAGTGCC 
ACTGACCATA AATTTCCTTC TTTGATGGAA AATTCAGATT CTCCGGTTAG AAGCATTCTG 
AAGTCCCAAG TCTGGCAGCC TTTGACTGAG GGCAGTGGAA ACAGAGGAAT GTTGAGAGAG 
CTGGGCGAGA CAGAAGGGAA GAGAGTCTTG GCAGGTGGAG ATGGTGGTAA AAAGTACGGG 
TCCTTTGAGG AAGTGGAGCC ACCCTGTCCT ACCCTGAACA GAGTCTGGGA AGGAGACAGC 
CATAAGGAAA CCAAATATGC TGTTCCAAGA AAGGGTAGCC TGGAACTAGC TCACCCTCCC 
GTTGCCCACC TTGGCGATGA ACTGAAGGAG TTCTCCGTGG GTAAAAGCAT TGCACAGGGG 
AGCCCAGACT GGACAGATAG GCAGCCCTTT GAAGAGAAGG TTGACCTGGA AAATGTCACG 
AGAAGGAGGT TTTCGCTAAG AGCAGAGTTT GGGGAGCCTG CTTCTGAGCA GACGGGAACA 
GCAGCCGGGA AAACCGTCAC CCAGACTGCA GCCCCCATGT CCTGGAAGCA GCATGATTCT 
GTAGAACCAG TACAGGAGAA GTACCCCAGG AATCCATGTG CCATGTTTGC TGCCGGAGAG 
ATCAAAGTGC CAGCGGTGGA GGGCATTCTG GATTCAGCCA GCAAAACCAT GTCAATTAAA 
GAAAGATTAG CGCTGTTGAA GAAGAGTGGT GAAGAAGATT GGAAGAACAG ACTGAGCAGA 
AAGCAGGAGT ACGGCAAGGC GTCTGTCTCC AGCAGCCTGC ACATACAAGA AGCGGAACAG 
TCCCTCAAGA AGAAGCGGGT CACAGAAAGT CGAGAGAGCC AGATGACTAT CGAAGAGAGG 
AAGCATCTCA TCACTGTGAG AGAAGAAGCT TGGAAGACAA AAGGCAAAGG AGCGGCCAAC 
GACTCCACCC AGTTTACGGT GGCTGGCAGA ATGGTGAAGA AAGGTTTGGC ATCACCCACC 
GCCATAACTC CAGTAGCGTC TCCTATTTGC AGTAAAACGA GGGGCACCAC ACCTGTTTCT 
AAACCTTTGG AAGATATTGA AGCCAGACCA GACATGCAGT TGGAATCGGA CCTGAAGTTG 
GACAGGCTGG AAACCTTTCT AAGAAGGCTG AATAACAAAG TTGGTGGGAT GCAGGAGACT 
GTACTCACTG TCACCGGTAA ATCTGTTAAA GAGGTGATGA AGTTGGATGA TGACGAGACC 
TTTGCCAGAT TTTACCGCAG CATGGATTAT ACTGTGCCAC GAAGCCCCGT GGAGCTAGAC 
GAAGATTTCG ATGTCATTTT CGATCCTTAT GCCCCTAAGT TGACCTCCTC TGTGGCCGAG 
CACAAGCGCG CAGTTAGGCC CAAGCGCCGG GTTCAGGCTT CCAGAAACCC CTTGAAAATG 
CTGGCAGCCA GAGAAGATCT CCTTCAGGAG TACACGGAGC AGAGACTAAA CATTGCCTTC 
ATGGAGTCAA AGCGGATGAA AGTAGAAAAG ATGTCCTCCA ACTCCAACTT CTCAGAAGTC 
ACTCTGGCAG GCTTAGCCAG TAAAGAAAAC TTCAGCAACA TCAGCCTGCG GAGCGTCAAC 
CTGACGGAAC AGAATTCCAA CAATAGCGCG GTACCCTATA AGAAGCTGAT GTTGTTACAG 
ATTAAAGGAA GAAGACACGT GCAGACAAGA CTGGTCGAAC CTCGAGCTTC ATCCCTCAAC 
AGTGGGGACT GCTTCCTCCT GCTCTCCCCT CACTACTGCT TCGTGTGGGT AGGAGAGTTT 
GCAAATGTCA TAGAAAAGGC AAAGGCCTCG GAACTTGCAA GTTTAATTCA GACAAAGAGG 
GAACTTGGTT GTAGAGCTAC ATATGTCCAA ACTATCGAAG AAGGAATTAA TACACACACT 
CATGCGGCCA AAGACTTCTG GAAGCTTCTG GGTGGCCAAA CCAGTTACCA GTCTGCCGGA 
GACCCAAAAG AAGATGAACT TTATGAAACA GCCATAATTG AAACAAACTG CATTTACCGT 
CTGATGGATG ACAAACTGGT CCCTGAGGAC GACTACTGGG GAAAGATTCC CAAGTGCTCC 
CTTCTGCAGT CAAAAGAGGT ACTGGTGTTT GATTTTGGTA GTGAAGTGTA CGTGTGGCAT 
GGAAAAGAAG TCACATTAGC GCAACGGAAA ATAGCTTTTC AACTGGCAAA GCACTTATGG 
AACGGAACCT TTGACTACGA GAACTGTGAC ATCAACCCGT TGGATCCTGG AGAATGCAAT 
CCGCTTATTC CCAGAAAAGG ACAAGGGCGA CCGGATTGGG CAATTTTCGG GAGACTTACA 
GAACACAATG AGACGATTTT GTTCAAAGAG AAATTTCTGG ATTGGACGGA ACTGAAGAGG 
CCTAATGAGA AGAGTGCCGG CGAACTCACC CAGCAAAAGG AAGATCCCAG GGCAGAAGCC 
AAGGCATATG ACGTGACACG GATGGTGCCG GCACCCCAGG CCACAACCGG CACTGTGCTG 
GATGGGGTGA ATGTTGGTCG TGGCTACGGC TTGGTGGATG GGGATGACAG GAGGCAGTTT 
GAGATCGCCA GTGTGTCTGT GGATGTGTGG CACATCCTAG AATTTGACTA CAGCAGGCTA 
CCCAAACAGA GCATCGGGCA GTTCCACGAG GGAGACGCCT ATGTGGTCAA GTGGAAGTAC 
ATGGTGAGCA CCGCAGTGGG AAGTCGACAA AAGGGAGAGC ACCCAGTACG GGTAGCTGGC 
AAGGAGAAGT GTGTCTACTT CTTCTGGCAA GGCCGGCAGT CGACCGTGAG TGAGAAGGGC 
ACATCAGCTC TGATGACAGT GGAGCTGGAT GAGGAGAGGG GTGCCCAGGT CCAGGTTCTG 
CAGGGAAAGG AGCCCCCCTG CTTTCTGCAG TGTTTCCAGG GGGGAATGGT GGTACACTCT 
GGGAGACGGG AAGAGGAGGA AGAAAATGCA CAAAGTGAGT GGAGGCTGTA CTGTGTCCGA 
GGAGAAGTGC CCATGGAAGG AAATTTGCTG GAAGTGGCCT GTCACTGTAG TAGCCTGAGG 
TCCAGGACTT CCATGGTTGT CCTCAACATA AACAAAGCCC TCATTTACTT ATGGCACGGA 
TGCAAAGCCC AAGCCCACAC GAAGGAGGTT GGAAGGACTG CAGCAAATAA AATCAAAGAA 
CAATGTCCCC TGGAAGCAGG ACTGCATAGT AGCAGCAAAG TGACAATACA CGAGTGTGAC 
GAAGGATCCG AGCCGCTGGG ATTCTGGGAT GCTTTAGGAA GGAGAGATAG AAAAGCGTAC 
GACTGTATGC TTCAAGATCC TGGAAATTTT AACTTCACAC CTCGCCTATT CATCCTCAGC 
AGTTCCTCCG GTGACTTCTT AGCCACAGAG TTCATATATC CCGCCCGAGA CCCCTCTGTG 
GTCAACTCCA TGCCTTTCCT GCAGGAAGAT CTTTACAGCG CGCCACAGCC AGCACTTTTC 
CTTGTTGACA ATCACCACGA GGTATACCTC TGGCAAGGCT GGTGGCCCAT AGAGAACAAG 
ATCACTGGAT CGGCCCGCAT TCGCTGGGCC TCCGACCGGA AGAGCGCCAT GGAAACCGTG 
CTCCAGTACT GCAAAGGAAA AAATATCAAG AGGCCGCCCC CCAAGTCTTA CCTTATCCAT 
GCTGGTCTGG AGCCCTTGAC ATTCACCAAC ATGTTCCCCA GCTGGGAGCA CAGAGAGGAC 
ATTGCTGAAA TCACAGAAAT GGATACCGAA GTTTCAAATC AGATCACCCT TGTGGAAGAT 
GTCTTAGCCA AGCTCTGTAA AACCATCTAT CCCCTGGCTG ATCTGCTAGC CAGGCCGCTA 
CCCGAGGGAG TTGATCCTCT GAAGCTTGAG ATTTATCTCA CCGATGAAGA CTTTGAGTTT 
GCACTAGACA TGACAAGAGA CGAATACAAT GCTCTGCCCG CCTGGAAGCA GGTGAACCTG 
AAGAAAGCAA AGGGTCTGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617049.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617106.1 Canis lupus familiaris supervillin (SVIL), transcript variant X6, mRNA.

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CloneID	OCe02723
Clone ID Related Accession (Same CDS sequence)	XM_005617049.2 , XM_005617049.3
Accession Version	XM_005617049.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617049.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACTG AACCTATCCC TGCTTCGTGC TCTGTGGCCA CCCACCCTGT AATGGCAAGA 
CTTCCTAGTC CCACTGTAGC TAAGAGCACT GTGCAGCCTG CCAGGTTGCA GGCATCTGCT 
CACCACAAAG CCTTAGCCAG AGACCAGGCA AATGAGGGCA AAGATGCTGC TGAACAAGGT 
GAACCTGACT CCTCCACTCT AAGCTTAGCA GAGAAGTTGG CCTTGTTTAA CAAATTATCC 
CAGCCAGTCT CAAAAGCTAT TTCTACCCGA AACAGAATAG ACATGAGACA GAGGAGAATG 
AATGCTCGCT ATCAGACTCA GCCAGTCACG CTTGGAGAGG TGGAACAGGT GCAAAGTGGA 
AAGCTCATTC CTTTCTCACC TACCGTTAAC ACATCTGTGT CCACCATGGC ATCTACAGTC 
ACTCCAATGT ATGCAGGGGA TCTTCGGACA AAGTTGCCTG TGGATGACAG AACAAGTGCC 
ACTGACCATA AATTTCCTTC TTTGATGGAA AATTCAGATT CTCCGGTTAG AAGCATTCTG 
AAGTCCCAAG TCTGGCAGCC TTTGACTGAG GGCAGTGGAA ACAGAGGAAT GTTGAGAGAG 
CTGGGCGAGA CAGAAGGGAA GAGAGTCTTG GCAGGTGGAG ATGGTGGTAA AAAGTACGGG 
TCCTTTGAGG AAGTGGAGCC ACCCTGTCCT ACCCTGAACA GAGTCTGGGA AGGAGACAGC 
CATAAGGAAA CCAAATATGC TGTTCCAAGA AAGGGTAGCC TGGAACTAGC TCACCCTCCC 
GTTGCCCACC TTGGCGATGA ACTGAAGGAG TTCTCCGTGG GTAAAAGCAT TGCACAGGGG 
AGCCCAGACT GGACAGATAG GCAGCCCTTT GAAGAGAAGG TTGACCTGGA AAATGTCACG 
AGAAGGAGGT TTTCGCTAAG AGCAGAGTTT GGGGAGCCTG CTTCTGAGCA GACGGGAACA 
GCAGCCGGGA AAACCGTCAC CCAGACTGCA GCCCCCATGT CCTGGAAGCA GCATGATTCT 
GTAGAACCAG TACAGGAGAA GTACCCCAGG AATCCATGTG CCATGTTTGC TGCCGGAGAG 
ATCAAAGTGC CAGCGGTGGA GGGCATTCTG GATTCAGCCA GCAAAACCAT GTCAATTAAA 
GAAAGATTAG CGCTGTTGAA GAAGAGTGGT GAAGAAGATT GGAAGAACAG ACTGAGCAGA 
AAGCAGGAGT ACGGCAAGGC GTCTGTCTCC AGCAGCCTGC ACATACAAGA AGCGGAACAG 
TCCCTCAAGA AGAAGCGGGT CACAGAAAGT CGAGAGAGCC AGATGACTAT CGAAGAGAGG 
AAGCATCTCA TCACTGTGAG AGAAGAAGCT TGGAAGACAA AAGGCAAAGG AGCGGCCAAC 
GACTCCACCC AGTTTACGGT GGCTGGCAGA ATGGTGAAGA AAGGTTTGGC ATCACCCACC 
GCCATAACTC CAGTAGCGTC TCCTATTTGC AGTAAAACGA GGGGCACCAC ACCTGTTTCT 
AAACCTTTGG AAGATATTGA AGCCAGACCA GACATGCAGT TGGAATCGGA CCTGAAGTTG 
GACAGGCTGG AAACCTTTCT AAGAAGGCTG AATAACAAAG TTGGTGGGAT GCAGGAGACT 
GTACTCACTG TCACCGGTAA ATCTGTTAAA GAGGTGATGA AGTTGGATGA TGACGAGACC 
TTTGCCAGAT TTTACCGCAG CATGGATTAT ACTGTGCCAC GAAGCCCCGT GGAGCTAGAC 
GAAGATTTCG ATGTCATTTT CGATCCTTAT GCCCCTAAGT TGACCTCCTC TGTGGCCGAG 
CACAAGCGCG CAGTTAGGCC CAAGCGCCGG GTTCAGGCTT CCAGAAACCC CTTGAAAATG 
CTGGCAGCCA GAGAAGATCT CCTTCAGGAG TACACGGAGC AGAGACTAAA CATTGCCTTC 
ATGGAGTCAA AGCGGATGAA AGTAGAAAAG ATGTCCTCCA ACTCCAACTT CTCAGAAGTC 
ACTCTGGCAG GCTTAGCCAG TAAAGAAAAC TTCAGCAACA TCAGCCTGCG GAGCGTCAAC 
CTGACGGAAC AGAATTCCAA CAATAGCGCG GTACCCTATA AGAAGCTGAT GTTGTTACAG 
ATTAAAGGAA GAAGACACGT GCAGACAAGA CTGGTCGAAC CTCGAGCTTC ATCCCTCAAC 
AGTGGGGACT GCTTCCTCCT GCTCTCCCCT CACTACTGCT TCGTGTGGGT AGGAGAGTTT 
GCAAATGTCA TAGAAAAGGC AAAGGCCTCG GAACTTGCAA GTTTAATTCA GACAAAGAGG 
GAACTTGGTT GTAGAGCTAC ATATGTCCAA ACTATCGAAG AAGGAATTAA TACACACACT 
CATGCGGCCA AAGACTTCTG GAAGCTTCTG GGTGGCCAAA CCAGTTACCA GTCTGCCGGA 
GACCCAAAAG AAGATGAACT TTATGAAACA GCCATAATTG AAACAAACTG CATTTACCGT 
CTGATGGATG ACAAACTGGT CCCTGAGGAC GACTACTGGG GAAAGATTCC CAAGTGCTCC 
CTTCTGCAGT CAAAAGAGGT ACTGGTGTTT GATTTTGGTA GTGAAGTGTA CGTGTGGCAT 
GGAAAAGAAG TCACATTAGC GCAACGGAAA ATAGCTTTTC AACTGGCAAA GCACTTATGG 
AACGGAACCT TTGACTACGA GAACTGTGAC ATCAACCCGT TGGATCCTGG AGAATGCAAT 
CCGCTTATTC CCAGAAAAGG ACAAGGGCGA CCGGATTGGG CAATTTTCGG GAGACTTACA 
GAACACAATG AGACGATTTT GTTCAAAGAG AAATTTCTGG ATTGGACGGA ACTGAAGAGG 
CCTAATGAGA AGAGTGCCGG CGAACTCACC CAGCAAAAGG AAGATCCCAG GGCAGAAGCC 
AAGGCATATG ACGTGACACG GATGGTGCCG GCACCCCAGG CCACAACCGG CACTGTGCTG 
GATGGGGTGA ATGTTGGTCG TGGCTACGGC TTGGTGGATG GGGATGACAG GAGGCAGTTT 
GAGATCGCCA GTGTGTCTGT GGATGTGTGG CACATCCTAG AATTTGACTA CAGCAGGCTA 
CCCAAACAGA GCATCGGGCA GTTCCACGAG GGAGACGCCT ATGTGGTCAA GTGGAAGTAC 
ATGGTGAGCA CCGCAGTGGG AAGTCGACAA AAGGGAGAGC ACCCAGTACG GGTAGCTGGC 
AAGGAGAAGT GTGTCTACTT CTTCTGGCAA GGCCGGCAGT CGACCGTGAG TGAGAAGGGC 
ACATCAGCTC TGATGACAGT GGAGCTGGAT GAGGAGAGGG GTGCCCAGGT CCAGGTTCTG 
CAGGGAAAGG AGCCCCCCTG CTTTCTGCAG TGTTTCCAGG GGGGAATGGT GGTACACTCT 
GGGAGACGGG AAGAGGAGGA AGAAAATGCA CAAAGTGAGT GGAGGCTGTA CTGTGTCCGA 
GGAGAAGTGC CCATGGAAGG AAATTTGCTG GAAGTGGCCT GTCACTGTAG TAGCCTGAGG 
TCCAGGACTT CCATGGTTGT CCTCAACATA AACAAAGCCC TCATTTACTT ATGGCACGGA 
TGCAAAGCCC AAGCCCACAC GAAGGAGGTT GGAAGGACTG CAGCAAATAA AATCAAAGAA 
CAATGTCCCC TGGAAGCAGG ACTGCATAGT AGCAGCAAAG TGACAATACA CGAGTGTGAC 
GAAGGATCCG AGCCGCTGGG ATTCTGGGAT GCTTTAGGAA GGAGAGATAG AAAAGCGTAC 
GACTGTATGC TTCAAGATCC TGGAAATTTT AACTTCACAC CTCGCCTATT CATCCTCAGC 
AGTTCCTCCG GTGACTTCTT AGCCACAGAG TTCATATATC CCGCCCGAGA CCCCTCTGTG 
GTCAACTCCA TGCCTTTCCT GCAGGAAGAT CTTTACAGCG CGCCACAGCC AGCACTTTTC 
CTTGTTGACA ATCACCACGA GGTATACCTC TGGCAAGGCT GGTGGCCCAT AGAGAACAAG 
ATCACTGGAT CGGCCCGCAT TCGCTGGGCC TCCGACCGGA AGAGCGCCAT GGAAACCGTG 
CTCCAGTACT GCAAAGGAAA AAATATCAAG AGGCCGCCCC CCAAGTCTTA CCTTATCCAT 
GCTGGTCTGG AGCCCTTGAC ATTCACCAAC ATGTTCCCCA GCTGGGAGCA CAGAGAGGAC 
ATTGCTGAAA TCACAGAAAT GGATACCGAA GTTTCAAATC AGATCACCCT TGTGGAAGAT 
GTCTTAGCCA AGCTCTGTAA AACCATCTAT CCCCTGGCTG ATCTGCTAGC CAGGCCGCTA 
CCCGAGGGAG TTGATCCTCT GAAGCTTGAG ATTTATCTCA CCGATGAAGA CTTTGAGTTT 
GCACTAGACA TGACAAGAGA CGAATACAAT GCTCTGCCCG CCTGGAAGCA GGTGAACCTG 
AAGAAAGCAA AGGGTCTGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617106.1
CDS	65..6928
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617049.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617049.3

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACTG AACCTATCCC TGCTTCGTGC TCTGTGGCCA CCCACCCTGT AATGGCAAGA 
CTTCCTAGTC CCACTGTAGC TAAGAGCACT GTGCAGCCTG CCAGGTTGCA GGCATCTGCT 
CACCACAAAG CCTTAGCCAG AGACCAGGCA AATGAGGGCA AAGATGCTGC TGAACAAGGT 
GAACCTGACT CCTCCACTCT AAGCTTAGCA GAGAAGTTGG CCTTGTTTAA CAAATTATCC 
CAGCCAGTCT CAAAAGCTAT TTCTACCCGA AACAGAATAG ACATGAGACA GAGGAGAATG 
AATGCTCGCT ATCAGACTCA GCCAGTCACG CTTGGAGAGG TGGAACAGGT GCAAAGTGGA 
AAGCTCATTC CTTTCTCACC TACCGTTAAC ACATCTGTGT CCACCATGGC ATCTACAGTC 
ACTCCAATGT ATGCAGGGGA TCTTCGGACA AAGTTGCCTG TGGATGACAG AACAAGTGCC 
ACTGACCATA AATTTCCTTC TTTGATGGAA AATTCAGATT CTCCGGTTAG AAGCATTCTG 
AAGTCCCAAG TCTGGCAGCC TTTGACTGAG GGCAGTGGAA ACAGAGGAAT GTTGAGAGAG 
CTGGGCGAGA CAGAAGGGAA GAGAGTCTTG GCAGGTGGAG ATGGTGGTAA AAAGTACGGG 
TCCTTTGAGG AAGTGGAGCC ACCCTGTCCT ACCCTGAACA GAGTCTGGGA AGGAGACAGC 
CATAAGGAAA CCAAATATGC TGTTCCAAGA AAGGGTAGCC TGGAACTAGC TCACCCTCCC 
GTTGCCCACC TTGGCGATGA ACTGAAGGAG TTCTCCGTGG GTAAAAGCAT TGCACAGGGG 
AGCCCAGACT GGACAGATAG GCAGCCCTTT GAAGAGAAGG TTGACCTGGA AAATGTCACG 
AGAAGGAGGT TTTCGCTAAG AGCAGAGTTT GGGGAGCCTG CTTCTGAGCA GACGGGAACA 
GCAGCCGGGA AAACCGTCAC CCAGACTGCA GCCCCCATGT CCTGGAAGCA GCATGATTCT 
GTAGAACCAG TACAGGAGAA GTACCCCAGG AATCCATGTG CCATGTTTGC TGCCGGAGAG 
ATCAAAGTGC CAGCGGTGGA GGGCATTCTG GATTCAGCCA GCAAAACCAT GTCAATTAAA 
GAAAGATTAG CGCTGTTGAA GAAGAGTGGT GAAGAAGATT GGAAGAACAG ACTGAGCAGA 
AAGCAGGAGT ACGGCAAGGC GTCTGTCTCC AGCAGCCTGC ACATACAAGA AGCGGAACAG 
TCCCTCAAGA AGAAGCGGGT CACAGAAAGT CGAGAGAGCC AGATGACTAT CGAAGAGAGG 
AAGCATCTCA TCACTGTGAG AGAAGAAGCT TGGAAGACAA AAGGCAAAGG AGCGGCCAAC 
GACTCCACCC AGTTTACGGT GGCTGGCAGA ATGGTGAAGA AAGGTTTGGC ATCACCCACC 
GCCATAACTC CAGTAGCGTC TCCTATTTGC AGTAAAACGA GGGGCACCAC ACCTGTTTCT 
AAACCTTTGG AAGATATTGA AGCCAGACCA GACATGCAGT TGGAATCGGA CCTGAAGTTG 
GACAGGCTGG AAACCTTTCT AAGAAGGCTG AATAACAAAG TTGGTGGGAT GCAGGAGACT 
GTACTCACTG TCACCGGTAA ATCTGTTAAA GAGGTGATGA AGTTGGATGA TGACGAGACC 
TTTGCCAGAT TTTACCGCAG CATGGATTAT ACTGTGCCAC GAAGCCCCGT GGAGCTAGAC 
GAAGATTTCG ATGTCATTTT CGATCCTTAT GCCCCTAAGT TGACCTCCTC TGTGGCCGAG 
CACAAGCGCG CAGTTAGGCC CAAGCGCCGG GTTCAGGCTT CCAGAAACCC CTTGAAAATG 
CTGGCAGCCA GAGAAGATCT CCTTCAGGAG TACACGGAGC AGAGACTAAA CATTGCCTTC 
ATGGAGTCAA AGCGGATGAA AGTAGAAAAG ATGTCCTCCA ACTCCAACTT CTCAGAAGTC 
ACTCTGGCAG GCTTAGCCAG TAAAGAAAAC TTCAGCAACA TCAGCCTGCG GAGCGTCAAC 
CTGACGGAAC AGAATTCCAA CAATAGCGCG GTACCCTATA AGAAGCTGAT GTTGTTACAG 
ATTAAAGGAA GAAGACACGT GCAGACAAGA CTGGTCGAAC CTCGAGCTTC ATCCCTCAAC 
AGTGGGGACT GCTTCCTCCT GCTCTCCCCT CACTACTGCT TCGTGTGGGT AGGAGAGTTT 
GCAAATGTCA TAGAAAAGGC AAAGGCCTCG GAACTTGCAA GTTTAATTCA GACAAAGAGG 
GAACTTGGTT GTAGAGCTAC ATATGTCCAA ACTATCGAAG AAGGAATTAA TACACACACT 
CATGCGGCCA AAGACTTCTG GAAGCTTCTG GGTGGCCAAA CCAGTTACCA GTCTGCCGGA 
GACCCAAAAG AAGATGAACT TTATGAAACA GCCATAATTG AAACAAACTG CATTTACCGT 
CTGATGGATG ACAAACTGGT CCCTGAGGAC GACTACTGGG GAAAGATTCC CAAGTGCTCC 
CTTCTGCAGT CAAAAGAGGT ACTGGTGTTT GATTTTGGTA GTGAAGTGTA CGTGTGGCAT 
GGAAAAGAAG TCACATTAGC GCAACGGAAA ATAGCTTTTC AACTGGCAAA GCACTTATGG 
AACGGAACCT TTGACTACGA GAACTGTGAC ATCAACCCGT TGGATCCTGG AGAATGCAAT 
CCGCTTATTC CCAGAAAAGG ACAAGGGCGA CCGGATTGGG CAATTTTCGG GAGACTTACA 
GAACACAATG AGACGATTTT GTTCAAAGAG AAATTTCTGG ATTGGACGGA ACTGAAGAGG 
CCTAATGAGA AGAGTGCCGG CGAACTCACC CAGCAAAAGG AAGATCCCAG GGCAGAAGCC 
AAGGCATATG ACGTGACACG GATGGTGCCG GCACCCCAGG CCACAACCGG CACTGTGCTG 
GATGGGGTGA ATGTTGGTCG TGGCTACGGC TTGGTGGATG GGGATGACAG GAGGCAGTTT 
GAGATCGCCA GTGTGTCTGT GGATGTGTGG CACATCCTAG AATTTGACTA CAGCAGGCTA 
CCCAAACAGA GCATCGGGCA GTTCCACGAG GGAGACGCCT ATGTGGTCAA GTGGAAGTAC 
ATGGTGAGCA CCGCAGTGGG AAGTCGACAA AAGGGAGAGC ACCCAGTACG GGTAGCTGGC 
AAGGAGAAGT GTGTCTACTT CTTCTGGCAA GGCCGGCAGT CGACCGTGAG TGAGAAGGGC 
ACATCAGCTC TGATGACAGT GGAGCTGGAT GAGGAGAGGG GTGCCCAGGT CCAGGTTCTG 
CAGGGAAAGG AGCCCCCCTG CTTTCTGCAG TGTTTCCAGG GGGGAATGGT GGTACACTCT 
GGGAGACGGG AAGAGGAGGA AGAAAATGCA CAAAGTGAGT GGAGGCTGTA CTGTGTCCGA 
GGAGAAGTGC CCATGGAAGG AAATTTGCTG GAAGTGGCCT GTCACTGTAG TAGCCTGAGG 
TCCAGGACTT CCATGGTTGT CCTCAACATA AACAAAGCCC TCATTTACTT ATGGCACGGA 
TGCAAAGCCC AAGCCCACAC GAAGGAGGTT GGAAGGACTG CAGCAAATAA AATCAAAGAA 
CAATGTCCCC TGGAAGCAGG ACTGCATAGT AGCAGCAAAG TGACAATACA CGAGTGTGAC 
GAAGGATCCG AGCCGCTGGG ATTCTGGGAT GCTTTAGGAA GGAGAGATAG AAAAGCGTAC 
GACTGTATGC TTCAAGATCC TGGAAATTTT AACTTCACAC CTCGCCTATT CATCCTCAGC 
AGTTCCTCCG GTGACTTCTT AGCCACAGAG TTCATATATC CCGCCCGAGA CCCCTCTGTG 
GTCAACTCCA TGCCTTTCCT GCAGGAAGAT CTTTACAGCG CGCCACAGCC AGCACTTTTC 
CTTGTTGACA ATCACCACGA GGTATACCTC TGGCAAGGCT GGTGGCCCAT AGAGAACAAG 
ATCACTGGAT CGGCCCGCAT TCGCTGGGCC TCCGACCGGA AGAGCGCCAT GGAAACCGTG 
CTCCAGTACT GCAAAGGAAA AAATATCAAG AGGCCGCCCC CCAAGTCTTA CCTTATCCAT 
GCTGGTCTGG AGCCCTTGAC ATTCACCAAC ATGTTCCCCA GCTGGGAGCA CAGAGAGGAC 
ATTGCTGAAA TCACAGAAAT GGATACCGAA GTTTCAAATC AGATCACCCT TGTGGAAGAT 
GTCTTAGCCA AGCTCTGTAA AACCATCTAT CCCCTGGCTG ATCTGCTAGC CAGGCCGCTA 
CCCGAGGGAG TTGATCCTCT GAAGCTTGAG ATTTATCTCA CCGATGAAGA CTTTGAGTTT 
GCACTAGACA TGACAAGAGA CGAATACAAT GCTCTGCCCG CCTGGAAGCA GGTGAACCTG 
AAGAAAGCAA AGGGTCTGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617050.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617107.1 Canis lupus familiaris supervillin (SVIL), transcript variant X7, mRNA.

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CloneID	OCe02724
Clone ID Related Accession (Same CDS sequence)	XM_005617050.3 , XM_005617050.2
Accession Version	XM_005617050.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617050.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT GTCTGCTTCG CATTCAGAAA TGCCAACCAC TGAGGATGAA 
GAAAAGGTAG ATGAACGAGC CAAGCTGAGT GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA 
ATGGAAAAAT CATTTGATGA GAAAAATGTT CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG 
GAGCAAAGGC TGCGTCGGCT ACAGGATAGA TCCCACACCC AGCCCGTTAC CACCGAGGAA 
GTGGCCATTG CAGCCACTGA ACCTATCCCT GCTTCGTGCT CTGTGGCCAC CCACCCTGTA 
ATGGCAAGAC TTCCTAGTCC CACTGTAGCT AAGAGCACTG TGCAGCCTGC CAGGTTGCAG 
GCATCTGCTC ACCACAAAGC CTTAGCCAGA GACCAGGCAA ATGAGGGCAA AGATGCTGCT 
GAACAAGGTG AACCTGACTC CTCCACTCTA AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC 
AAATTATCCC AGCCAGTCTC AAAAGCTATT TCTACCCGAA ACAGAATAGA CATGAGACAG 
AGGAGAATGA ATGCTCGCTA TCAGACTCAG CCAGTCACGC TTGGAGAGGT GGAACAGGTG 
CAAAGTGGAA AGCTCATTCC TTTCTCACCT ACCGTTAACA CATCTGTGTC CACCATGGCA 
TCTACAGTCA CTCCAATGTA TGCAGGGGAT CTTCGGACAA AGTTGCCTGT GGATGACAGA 
ACAAGTGCCA CTGACCATAA ATTTCCTTCT TTGATGGAAA ATTCAGATTC TCCGGTTAGA 
AGCATTCTGA AGTCCCAAGT CTGGCAGCCT TTGACTGAGG GCAGTGGAAA CAGAGGAATG 
TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA 
AAGTACGGGT CCTTTGAGGA AGTGGAGCCA CCCTGTCCTA CCCTGAACAG AGTCTGGGAA 
GGAGACAGCC ATAAGGAAAC CAAATATGCT GTTCCAAGAA AGGGTAGCCT GGAACTAGCT 
CACCCTCCCG TTGCCCACCT TGGCGATGAA CTGAAGGAGT TCTCCGTGGG TAAAAGCATT 
GCACAGGGGA GCCCAGACTG GACAGATAGG CAGCCCTTTG AAGAGAAGGT TGACCTGGAA 
AATGTCACGA GAAGGAGGTT TTCGCTAAGA GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG 
ACGGGAACAG CAGCCGGGAA AACCGTCACC CAGACTGCAG CCCCCATGTC CTGGAAGCAG 
CATGATTCTG TAGAACCAGT ACAGGAGAAG TACCCCAGGA ATCCATGTGC CATGTTTGCT 
GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG GGCATTCTGG ATTCAGCCAG CAAAACCATG 
TCAATTAAAG AAAGATTAGC GCTGTTGAAG AAGAGTGGTG AAGAAGATTG GAAGAACAGA 
CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG TCTGTCTCCA GCAGCCTGCA CATACAAGAA 
GCGGAACAGT CCCTCAAGAA GAAGGAAGAA GGAGGATTTG CAGATGATAG TGCCATTTCT 
AATCTGCTTT GGGAACCTGT ATATGCTTCT ACTTATTCTC CTGCTATACC TGCTGCCCAT 
AAACACCTGT CTTTTGTATC TATTAATCAG CGGGTCACAG AAAGTCGAGA GAGCCAGATG 
ACTATCGAAG AGAGGAAGCA TCTCATCACT GTGAGAGAAG AAGCTTGGAA GACAAAAGGC 
AAAGGAGCGG CCAACGACTC CACCCAGTTT ACGGTGGCTG GCAGAATGGT GAAGAAAGGT 
TTGGCATCAC CCACCGCCAT AACTCCAGTA GCGTCTCCTA TTTGCAGTAA AACGAGGGGC 
ACCACACCTG TTTCTAAACC TTTGGAAGAT ATTGAAGCCA GACCAGACAT GCAGTTGGAA 
TCGGACCTGA AGTTGGACAG GCTGGAAACC TTTCTAAGAA GGCTGAATAA CAAAGTTGGT 
GGGATGCAGG AGACTGTACT CACTGTCACC GGTAAATCTG TTAAAGAGGT GATGAAGTTG 
GATGATGACG AGACCTTTGC CAGATTTTAC CGCAGCATGG ATTATACTGT GCCACGAAGC 
CCCGTGGAGC TAGACGAAGA TTTCGATGTC ATTTTCGATC CTTATGCCCC TAAGTTGACC 
TCCTCTGTGG CCGAGCACAA GCGCGCAGTT AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA 
AACCCCTTGA AAATGCTGGC AGCCAGAGAA GATCTCCTTC AGGAGTACAC GGAGCAGAGA 
CTAAACATTG CCTTCATGGA GTCAAAGCGG ATGAAAGTAG AAAAGATGTC CTCCAACTCC 
AACTTCTCAG AAGTCACTCT GGCAGGCTTA GCCAGTAAAG AAAACTTCAG CAACATCAGC 
CTGCGGAGCG TCAACCTGAC GGAACAGAAT TCCAACAATA GCGCGGTACC CTATAAGAAG 
CTGATGTTGT TACAGATTAA AGGAAGAAGA CACGTGCAGA CAAGACTGGT CGAACCTCGA 
GCTTCATCCC TCAACAGTGG GGACTGCTTC CTCCTGCTCT CCCCTCACTA CTGCTTCGTG 
TGGGTAGGAG AGTTTGCAAA TGTCATAGAA AAGGCAAAGG CCTCGGAACT TGCAAGTTTA 
ATTCAGACAA AGAGGGAACT TGGTTGTAGA GCTACATATG TCCAAACTAT CGAAGAAGGA 
ATTAATACAC ACACTCATGC GGCCAAAGAC TTCTGGAAGC TTCTGGGTGG CCAAACCAGT 
TACCAGTCTG CCGGAGACCC AAAAGAAGAT GAACTTTATG AAACAGCCAT AATTGAAACA 
AACTGCATTT ACCGTCTGAT GGATGACAAA CTGGTCCCTG AGGACGACTA CTGGGGAAAG 
ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA GAGGTACTGG TGTTTGATTT TGGTAGTGAA 
GTGTACGTGT GGCATGGAAA AGAAGTCACA TTAGCGCAAC GGAAAATAGC TTTTCAACTG 
GCAAAGCACT TATGGAACGG AACCTTTGAC TACGAGAACT GTGACATCAA CCCGTTGGAT 
CCTGGAGAAT GCAATCCGCT TATTCCCAGA AAAGGACAAG GGCGACCGGA TTGGGCAATT 
TTCGGGAGAC TTACAGAACA CAATGAGACG ATTTTGTTCA AAGAGAAATT TCTGGATTGG 
ACGGAACTGA AGAGGCCTAA TGAGAAGAGT GCCGGCGAAC TCACCCAGCA AAAGGAAGAT 
CCCAGGGCAG AAGCCAAGGC ATATGACGTG ACACGGATGG TGCCGGCACC CCAGGCCACA 
ACCGGCACTG TGCTGGATGG GGTGAATGTT GGTCGTGGCT ACGGCTTGGT GGATGGGGAT 
GACAGGAGGC AGTTTGAGAT CGCCAGTGTG TCTGTGGATG TGTGGCACAT CCTAGAATTT 
GACTACAGCA GGCTACCCAA ACAGAGCATC GGGCAGTTCC ACGAGGGAGA CGCCTATGTG 
GTCAAGTGGA AGTACATGGT GAGCACCGCA GTGGGAAGTC GACAAAAGGG AGAGCACCCA 
GTACGGGTAG CTGGCAAGGA GAAGTGTGTC TACTTCTTCT GGCAAGGCCG GCAGTCGACC 
GTGAGTGAGA AGGGCACATC AGCTCTGATG ACAGTGGAGC TGGATGAGGA GAGGGGTGCC 
CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA 
ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG GAGGAAGAAA ATGCACAAAG TGAGTGGAGG 
CTGTACTGTG TCCGAGGAGA AGTGCCCATG GAAGGAAATT TGCTGGAAGT GGCCTGTCAC 
TGTAGTAGCC TGAGGTCCAG GACTTCCATG GTTGTCCTCA ACATAAACAA AGCCCTCATT 
TACTTATGGC ACGGATGCAA AGCCCAAGCC CACACGAAGG AGGTTGGAAG GACTGCAGCA 
AATAAAATCA AAGAACAATG TCCCCTGGAA GCAGGACTGC ATAGTAGCAG CAAAGTGACA 
ATACACGAGT GTGACGAAGG ATCCGAGCCG CTGGGATTCT GGGATGCTTT AGGAAGGAGA 
GATAGAAAAG CGTACGACTG TATGCTTCAA GATCCTGGAA ATTTTAACTT CACACCTCGC 
CTATTCATCC TCAGCAGTTC CTCCGGTGAC TTCTTAGCCA CAGAGTTCAT ATATCCCGCC 
CGAGACCCCT CTGTGGTCAA CTCCATGCCT TTCCTGCAGG AAGATCTTTA CAGCGCGCCA 
CAGCCAGCAC TTTTCCTTGT TGACAATCAC CACGAGGTAT ACCTCTGGCA AGGCTGGTGG 
CCCATAGAGA ACAAGATCAC TGGATCGGCC CGCATTCGCT GGGCCTCCGA CCGGAAGAGC 
GCCATGGAAA CCGTGCTCCA GTACTGCAAA GGAAAAAATA TCAAGAGGCC GCCCCCCAAG 
TCTTACCTTA TCCATGCTGG TCTGGAGCCC TTGACATTCA CCAACATGTT CCCCAGCTGG 
GAGCACAGAG AGGACATTGC TGAAATCACA GAAATGGATA CCGAAGTTTC AAATCAGATC 
ACCCTTGTGG AAGATGTCTT AGCCAAGCTC TGTAAAACCA TCTATCCCCT GGCTGATCTG 
CTAGCCAGGC CGCTACCCGA GGGAGTTGAT CCTCTGAAGC TTGAGATTTA TCTCACCGAT 
GAAGACTTTG AGTTTGCACT AGACATGACA AGAGACGAAT ACAATGCTCT GCCCGCCTGG 
AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617107.1
CDS	65..6883
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617050.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617050.3

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT GTCTGCTTCG CATTCAGAAA TGCCAACCAC TGAGGATGAA 
GAAAAGGTAG ATGAACGAGC CAAGCTGAGT GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA 
ATGGAAAAAT CATTTGATGA GAAAAATGTT CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG 
GAGCAAAGGC TGCGTCGGCT ACAGGATAGA TCCCACACCC AGCCCGTTAC CACCGAGGAA 
GTGGCCATTG CAGCCACTGA ACCTATCCCT GCTTCGTGCT CTGTGGCCAC CCACCCTGTA 
ATGGCAAGAC TTCCTAGTCC CACTGTAGCT AAGAGCACTG TGCAGCCTGC CAGGTTGCAG 
GCATCTGCTC ACCACAAAGC CTTAGCCAGA GACCAGGCAA ATGAGGGCAA AGATGCTGCT 
GAACAAGGTG AACCTGACTC CTCCACTCTA AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC 
AAATTATCCC AGCCAGTCTC AAAAGCTATT TCTACCCGAA ACAGAATAGA CATGAGACAG 
AGGAGAATGA ATGCTCGCTA TCAGACTCAG CCAGTCACGC TTGGAGAGGT GGAACAGGTG 
CAAAGTGGAA AGCTCATTCC TTTCTCACCT ACCGTTAACA CATCTGTGTC CACCATGGCA 
TCTACAGTCA CTCCAATGTA TGCAGGGGAT CTTCGGACAA AGTTGCCTGT GGATGACAGA 
ACAAGTGCCA CTGACCATAA ATTTCCTTCT TTGATGGAAA ATTCAGATTC TCCGGTTAGA 
AGCATTCTGA AGTCCCAAGT CTGGCAGCCT TTGACTGAGG GCAGTGGAAA CAGAGGAATG 
TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA 
AAGTACGGGT CCTTTGAGGA AGTGGAGCCA CCCTGTCCTA CCCTGAACAG AGTCTGGGAA 
GGAGACAGCC ATAAGGAAAC CAAATATGCT GTTCCAAGAA AGGGTAGCCT GGAACTAGCT 
CACCCTCCCG TTGCCCACCT TGGCGATGAA CTGAAGGAGT TCTCCGTGGG TAAAAGCATT 
GCACAGGGGA GCCCAGACTG GACAGATAGG CAGCCCTTTG AAGAGAAGGT TGACCTGGAA 
AATGTCACGA GAAGGAGGTT TTCGCTAAGA GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG 
ACGGGAACAG CAGCCGGGAA AACCGTCACC CAGACTGCAG CCCCCATGTC CTGGAAGCAG 
CATGATTCTG TAGAACCAGT ACAGGAGAAG TACCCCAGGA ATCCATGTGC CATGTTTGCT 
GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG GGCATTCTGG ATTCAGCCAG CAAAACCATG 
TCAATTAAAG AAAGATTAGC GCTGTTGAAG AAGAGTGGTG AAGAAGATTG GAAGAACAGA 
CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG TCTGTCTCCA GCAGCCTGCA CATACAAGAA 
GCGGAACAGT CCCTCAAGAA GAAGGAAGAA GGAGGATTTG CAGATGATAG TGCCATTTCT 
AATCTGCTTT GGGAACCTGT ATATGCTTCT ACTTATTCTC CTGCTATACC TGCTGCCCAT 
AAACACCTGT CTTTTGTATC TATTAATCAG CGGGTCACAG AAAGTCGAGA GAGCCAGATG 
ACTATCGAAG AGAGGAAGCA TCTCATCACT GTGAGAGAAG AAGCTTGGAA GACAAAAGGC 
AAAGGAGCGG CCAACGACTC CACCCAGTTT ACGGTGGCTG GCAGAATGGT GAAGAAAGGT 
TTGGCATCAC CCACCGCCAT AACTCCAGTA GCGTCTCCTA TTTGCAGTAA AACGAGGGGC 
ACCACACCTG TTTCTAAACC TTTGGAAGAT ATTGAAGCCA GACCAGACAT GCAGTTGGAA 
TCGGACCTGA AGTTGGACAG GCTGGAAACC TTTCTAAGAA GGCTGAATAA CAAAGTTGGT 
GGGATGCAGG AGACTGTACT CACTGTCACC GGTAAATCTG TTAAAGAGGT GATGAAGTTG 
GATGATGACG AGACCTTTGC CAGATTTTAC CGCAGCATGG ATTATACTGT GCCACGAAGC 
CCCGTGGAGC TAGACGAAGA TTTCGATGTC ATTTTCGATC CTTATGCCCC TAAGTTGACC 
TCCTCTGTGG CCGAGCACAA GCGCGCAGTT AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA 
AACCCCTTGA AAATGCTGGC AGCCAGAGAA GATCTCCTTC AGGAGTACAC GGAGCAGAGA 
CTAAACATTG CCTTCATGGA GTCAAAGCGG ATGAAAGTAG AAAAGATGTC CTCCAACTCC 
AACTTCTCAG AAGTCACTCT GGCAGGCTTA GCCAGTAAAG AAAACTTCAG CAACATCAGC 
CTGCGGAGCG TCAACCTGAC GGAACAGAAT TCCAACAATA GCGCGGTACC CTATAAGAAG 
CTGATGTTGT TACAGATTAA AGGAAGAAGA CACGTGCAGA CAAGACTGGT CGAACCTCGA 
GCTTCATCCC TCAACAGTGG GGACTGCTTC CTCCTGCTCT CCCCTCACTA CTGCTTCGTG 
TGGGTAGGAG AGTTTGCAAA TGTCATAGAA AAGGCAAAGG CCTCGGAACT TGCAAGTTTA 
ATTCAGACAA AGAGGGAACT TGGTTGTAGA GCTACATATG TCCAAACTAT CGAAGAAGGA 
ATTAATACAC ACACTCATGC GGCCAAAGAC TTCTGGAAGC TTCTGGGTGG CCAAACCAGT 
TACCAGTCTG CCGGAGACCC AAAAGAAGAT GAACTTTATG AAACAGCCAT AATTGAAACA 
AACTGCATTT ACCGTCTGAT GGATGACAAA CTGGTCCCTG AGGACGACTA CTGGGGAAAG 
ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA GAGGTACTGG TGTTTGATTT TGGTAGTGAA 
GTGTACGTGT GGCATGGAAA AGAAGTCACA TTAGCGCAAC GGAAAATAGC TTTTCAACTG 
GCAAAGCACT TATGGAACGG AACCTTTGAC TACGAGAACT GTGACATCAA CCCGTTGGAT 
CCTGGAGAAT GCAATCCGCT TATTCCCAGA AAAGGACAAG GGCGACCGGA TTGGGCAATT 
TTCGGGAGAC TTACAGAACA CAATGAGACG ATTTTGTTCA AAGAGAAATT TCTGGATTGG 
ACGGAACTGA AGAGGCCTAA TGAGAAGAGT GCCGGCGAAC TCACCCAGCA AAAGGAAGAT 
CCCAGGGCAG AAGCCAAGGC ATATGACGTG ACACGGATGG TGCCGGCACC CCAGGCCACA 
ACCGGCACTG TGCTGGATGG GGTGAATGTT GGTCGTGGCT ACGGCTTGGT GGATGGGGAT 
GACAGGAGGC AGTTTGAGAT CGCCAGTGTG TCTGTGGATG TGTGGCACAT CCTAGAATTT 
GACTACAGCA GGCTACCCAA ACAGAGCATC GGGCAGTTCC ACGAGGGAGA CGCCTATGTG 
GTCAAGTGGA AGTACATGGT GAGCACCGCA GTGGGAAGTC GACAAAAGGG AGAGCACCCA 
GTACGGGTAG CTGGCAAGGA GAAGTGTGTC TACTTCTTCT GGCAAGGCCG GCAGTCGACC 
GTGAGTGAGA AGGGCACATC AGCTCTGATG ACAGTGGAGC TGGATGAGGA GAGGGGTGCC 
CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA 
ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG GAGGAAGAAA ATGCACAAAG TGAGTGGAGG 
CTGTACTGTG TCCGAGGAGA AGTGCCCATG GAAGGAAATT TGCTGGAAGT GGCCTGTCAC 
TGTAGTAGCC TGAGGTCCAG GACTTCCATG GTTGTCCTCA ACATAAACAA AGCCCTCATT 
TACTTATGGC ACGGATGCAA AGCCCAAGCC CACACGAAGG AGGTTGGAAG GACTGCAGCA 
AATAAAATCA AAGAACAATG TCCCCTGGAA GCAGGACTGC ATAGTAGCAG CAAAGTGACA 
ATACACGAGT GTGACGAAGG ATCCGAGCCG CTGGGATTCT GGGATGCTTT AGGAAGGAGA 
GATAGAAAAG CGTACGACTG TATGCTTCAA GATCCTGGAA ATTTTAACTT CACACCTCGC 
CTATTCATCC TCAGCAGTTC CTCCGGTGAC TTCTTAGCCA CAGAGTTCAT ATATCCCGCC 
CGAGACCCCT CTGTGGTCAA CTCCATGCCT TTCCTGCAGG AAGATCTTTA CAGCGCGCCA 
CAGCCAGCAC TTTTCCTTGT TGACAATCAC CACGAGGTAT ACCTCTGGCA AGGCTGGTGG 
CCCATAGAGA ACAAGATCAC TGGATCGGCC CGCATTCGCT GGGCCTCCGA CCGGAAGAGC 
GCCATGGAAA CCGTGCTCCA GTACTGCAAA GGAAAAAATA TCAAGAGGCC GCCCCCCAAG 
TCTTACCTTA TCCATGCTGG TCTGGAGCCC TTGACATTCA CCAACATGTT CCCCAGCTGG 
GAGCACAGAG AGGACATTGC TGAAATCACA GAAATGGATA CCGAAGTTTC AAATCAGATC 
ACCCTTGTGG AAGATGTCTT AGCCAAGCTC TGTAAAACCA TCTATCCCCT GGCTGATCTG 
CTAGCCAGGC CGCTACCCGA GGGAGTTGAT CCTCTGAAGC TTGAGATTTA TCTCACCGAT 
GAAGACTTTG AGTTTGCACT AGACATGACA AGAGACGAAT ACAATGCTCT GCCCGCCTGG 
AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617050.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617107.1 Canis lupus familiaris supervillin (SVIL), transcript variant X6, mRNA.

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CloneID	OCe02724
Clone ID Related Accession (Same CDS sequence)	XM_005617050.3 , XM_005617050.2
Accession Version	XM_005617050.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	supervillin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726048.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617050.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT GTCTGCTTCG CATTCAGAAA TGCCAACCAC TGAGGATGAA 
GAAAAGGTAG ATGAACGAGC CAAGCTGAGT GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA 
ATGGAAAAAT CATTTGATGA GAAAAATGTT CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG 
GAGCAAAGGC TGCGTCGGCT ACAGGATAGA TCCCACACCC AGCCCGTTAC CACCGAGGAA 
GTGGCCATTG CAGCCACTGA ACCTATCCCT GCTTCGTGCT CTGTGGCCAC CCACCCTGTA 
ATGGCAAGAC TTCCTAGTCC CACTGTAGCT AAGAGCACTG TGCAGCCTGC CAGGTTGCAG 
GCATCTGCTC ACCACAAAGC CTTAGCCAGA GACCAGGCAA ATGAGGGCAA AGATGCTGCT 
GAACAAGGTG AACCTGACTC CTCCACTCTA AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC 
AAATTATCCC AGCCAGTCTC AAAAGCTATT TCTACCCGAA ACAGAATAGA CATGAGACAG 
AGGAGAATGA ATGCTCGCTA TCAGACTCAG CCAGTCACGC TTGGAGAGGT GGAACAGGTG 
CAAAGTGGAA AGCTCATTCC TTTCTCACCT ACCGTTAACA CATCTGTGTC CACCATGGCA 
TCTACAGTCA CTCCAATGTA TGCAGGGGAT CTTCGGACAA AGTTGCCTGT GGATGACAGA 
ACAAGTGCCA CTGACCATAA ATTTCCTTCT TTGATGGAAA ATTCAGATTC TCCGGTTAGA 
AGCATTCTGA AGTCCCAAGT CTGGCAGCCT TTGACTGAGG GCAGTGGAAA CAGAGGAATG 
TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA 
AAGTACGGGT CCTTTGAGGA AGTGGAGCCA CCCTGTCCTA CCCTGAACAG AGTCTGGGAA 
GGAGACAGCC ATAAGGAAAC CAAATATGCT GTTCCAAGAA AGGGTAGCCT GGAACTAGCT 
CACCCTCCCG TTGCCCACCT TGGCGATGAA CTGAAGGAGT TCTCCGTGGG TAAAAGCATT 
GCACAGGGGA GCCCAGACTG GACAGATAGG CAGCCCTTTG AAGAGAAGGT TGACCTGGAA 
AATGTCACGA GAAGGAGGTT TTCGCTAAGA GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG 
ACGGGAACAG CAGCCGGGAA AACCGTCACC CAGACTGCAG CCCCCATGTC CTGGAAGCAG 
CATGATTCTG TAGAACCAGT ACAGGAGAAG TACCCCAGGA ATCCATGTGC CATGTTTGCT 
GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG GGCATTCTGG ATTCAGCCAG CAAAACCATG 
TCAATTAAAG AAAGATTAGC GCTGTTGAAG AAGAGTGGTG AAGAAGATTG GAAGAACAGA 
CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG TCTGTCTCCA GCAGCCTGCA CATACAAGAA 
GCGGAACAGT CCCTCAAGAA GAAGGAAGAA GGAGGATTTG CAGATGATAG TGCCATTTCT 
AATCTGCTTT GGGAACCTGT ATATGCTTCT ACTTATTCTC CTGCTATACC TGCTGCCCAT 
AAACACCTGT CTTTTGTATC TATTAATCAG CGGGTCACAG AAAGTCGAGA GAGCCAGATG 
ACTATCGAAG AGAGGAAGCA TCTCATCACT GTGAGAGAAG AAGCTTGGAA GACAAAAGGC 
AAAGGAGCGG CCAACGACTC CACCCAGTTT ACGGTGGCTG GCAGAATGGT GAAGAAAGGT 
TTGGCATCAC CCACCGCCAT AACTCCAGTA GCGTCTCCTA TTTGCAGTAA AACGAGGGGC 
ACCACACCTG TTTCTAAACC TTTGGAAGAT ATTGAAGCCA GACCAGACAT GCAGTTGGAA 
TCGGACCTGA AGTTGGACAG GCTGGAAACC TTTCTAAGAA GGCTGAATAA CAAAGTTGGT 
GGGATGCAGG AGACTGTACT CACTGTCACC GGTAAATCTG TTAAAGAGGT GATGAAGTTG 
GATGATGACG AGACCTTTGC CAGATTTTAC CGCAGCATGG ATTATACTGT GCCACGAAGC 
CCCGTGGAGC TAGACGAAGA TTTCGATGTC ATTTTCGATC CTTATGCCCC TAAGTTGACC 
TCCTCTGTGG CCGAGCACAA GCGCGCAGTT AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA 
AACCCCTTGA AAATGCTGGC AGCCAGAGAA GATCTCCTTC AGGAGTACAC GGAGCAGAGA 
CTAAACATTG CCTTCATGGA GTCAAAGCGG ATGAAAGTAG AAAAGATGTC CTCCAACTCC 
AACTTCTCAG AAGTCACTCT GGCAGGCTTA GCCAGTAAAG AAAACTTCAG CAACATCAGC 
CTGCGGAGCG TCAACCTGAC GGAACAGAAT TCCAACAATA GCGCGGTACC CTATAAGAAG 
CTGATGTTGT TACAGATTAA AGGAAGAAGA CACGTGCAGA CAAGACTGGT CGAACCTCGA 
GCTTCATCCC TCAACAGTGG GGACTGCTTC CTCCTGCTCT CCCCTCACTA CTGCTTCGTG 
TGGGTAGGAG AGTTTGCAAA TGTCATAGAA AAGGCAAAGG CCTCGGAACT TGCAAGTTTA 
ATTCAGACAA AGAGGGAACT TGGTTGTAGA GCTACATATG TCCAAACTAT CGAAGAAGGA 
ATTAATACAC ACACTCATGC GGCCAAAGAC TTCTGGAAGC TTCTGGGTGG CCAAACCAGT 
TACCAGTCTG CCGGAGACCC AAAAGAAGAT GAACTTTATG AAACAGCCAT AATTGAAACA 
AACTGCATTT ACCGTCTGAT GGATGACAAA CTGGTCCCTG AGGACGACTA CTGGGGAAAG 
ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA GAGGTACTGG TGTTTGATTT TGGTAGTGAA 
GTGTACGTGT GGCATGGAAA AGAAGTCACA TTAGCGCAAC GGAAAATAGC TTTTCAACTG 
GCAAAGCACT TATGGAACGG AACCTTTGAC TACGAGAACT GTGACATCAA CCCGTTGGAT 
CCTGGAGAAT GCAATCCGCT TATTCCCAGA AAAGGACAAG GGCGACCGGA TTGGGCAATT 
TTCGGGAGAC TTACAGAACA CAATGAGACG ATTTTGTTCA AAGAGAAATT TCTGGATTGG 
ACGGAACTGA AGAGGCCTAA TGAGAAGAGT GCCGGCGAAC TCACCCAGCA AAAGGAAGAT 
CCCAGGGCAG AAGCCAAGGC ATATGACGTG ACACGGATGG TGCCGGCACC CCAGGCCACA 
ACCGGCACTG TGCTGGATGG GGTGAATGTT GGTCGTGGCT ACGGCTTGGT GGATGGGGAT 
GACAGGAGGC AGTTTGAGAT CGCCAGTGTG TCTGTGGATG TGTGGCACAT CCTAGAATTT 
GACTACAGCA GGCTACCCAA ACAGAGCATC GGGCAGTTCC ACGAGGGAGA CGCCTATGTG 
GTCAAGTGGA AGTACATGGT GAGCACCGCA GTGGGAAGTC GACAAAAGGG AGAGCACCCA 
GTACGGGTAG CTGGCAAGGA GAAGTGTGTC TACTTCTTCT GGCAAGGCCG GCAGTCGACC 
GTGAGTGAGA AGGGCACATC AGCTCTGATG ACAGTGGAGC TGGATGAGGA GAGGGGTGCC 
CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA 
ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG GAGGAAGAAA ATGCACAAAG TGAGTGGAGG 
CTGTACTGTG TCCGAGGAGA AGTGCCCATG GAAGGAAATT TGCTGGAAGT GGCCTGTCAC 
TGTAGTAGCC TGAGGTCCAG GACTTCCATG GTTGTCCTCA ACATAAACAA AGCCCTCATT 
TACTTATGGC ACGGATGCAA AGCCCAAGCC CACACGAAGG AGGTTGGAAG GACTGCAGCA 
AATAAAATCA AAGAACAATG TCCCCTGGAA GCAGGACTGC ATAGTAGCAG CAAAGTGACA 
ATACACGAGT GTGACGAAGG ATCCGAGCCG CTGGGATTCT GGGATGCTTT AGGAAGGAGA 
GATAGAAAAG CGTACGACTG TATGCTTCAA GATCCTGGAA ATTTTAACTT CACACCTCGC 
CTATTCATCC TCAGCAGTTC CTCCGGTGAC TTCTTAGCCA CAGAGTTCAT ATATCCCGCC 
CGAGACCCCT CTGTGGTCAA CTCCATGCCT TTCCTGCAGG AAGATCTTTA CAGCGCGCCA 
CAGCCAGCAC TTTTCCTTGT TGACAATCAC CACGAGGTAT ACCTCTGGCA AGGCTGGTGG 
CCCATAGAGA ACAAGATCAC TGGATCGGCC CGCATTCGCT GGGCCTCCGA CCGGAAGAGC 
GCCATGGAAA CCGTGCTCCA GTACTGCAAA GGAAAAAATA TCAAGAGGCC GCCCCCCAAG 
TCTTACCTTA TCCATGCTGG TCTGGAGCCC TTGACATTCA CCAACATGTT CCCCAGCTGG 
GAGCACAGAG AGGACATTGC TGAAATCACA GAAATGGATA CCGAAGTTTC AAATCAGATC 
ACCCTTGTGG AAGATGTCTT AGCCAAGCTC TGTAAAACCA TCTATCCCCT GGCTGATCTG 
CTAGCCAGGC CGCTACCCGA GGGAGTTGAT CCTCTGAAGC TTGAGATTTA TCTCACCGAT 
GAAGACTTTG AGTTTGCACT AGACATGACA AGAGACGAAT ACAATGCTCT GCCCGCCTGG 
AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617107.1
CDS	48..6866
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617050.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617050.2

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT GTCTGCTTCG CATTCAGAAA TGCCAACCAC TGAGGATGAA 
GAAAAGGTAG ATGAACGAGC CAAGCTGAGT GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA 
ATGGAAAAAT CATTTGATGA GAAAAATGTT CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG 
GAGCAAAGGC TGCGTCGGCT ACAGGATAGA TCCCACACCC AGCCCGTTAC CACCGAGGAA 
GTGGCCATTG CAGCCACTGA ACCTATCCCT GCTTCGTGCT CTGTGGCCAC CCACCCTGTA 
ATGGCAAGAC TTCCTAGTCC CACTGTAGCT AAGAGCACTG TGCAGCCTGC CAGGTTGCAG 
GCATCTGCTC ACCACAAAGC CTTAGCCAGA GACCAGGCAA ATGAGGGCAA AGATGCTGCT 
GAACAAGGTG AACCTGACTC CTCCACTCTA AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC 
AAATTATCCC AGCCAGTCTC AAAAGCTATT TCTACCCGAA ACAGAATAGA CATGAGACAG 
AGGAGAATGA ATGCTCGCTA TCAGACTCAG CCAGTCACGC TTGGAGAGGT GGAACAGGTG 
CAAAGTGGAA AGCTCATTCC TTTCTCACCT ACCGTTAACA CATCTGTGTC CACCATGGCA 
TCTACAGTCA CTCCAATGTA TGCAGGGGAT CTTCGGACAA AGTTGCCTGT GGATGACAGA 
ACAAGTGCCA CTGACCATAA ATTTCCTTCT TTGATGGAAA ATTCAGATTC TCCGGTTAGA 
AGCATTCTGA AGTCCCAAGT CTGGCAGCCT TTGACTGAGG GCAGTGGAAA CAGAGGAATG 
TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA 
AAGTACGGGT CCTTTGAGGA AGTGGAGCCA CCCTGTCCTA CCCTGAACAG AGTCTGGGAA 
GGAGACAGCC ATAAGGAAAC CAAATATGCT GTTCCAAGAA AGGGTAGCCT GGAACTAGCT 
CACCCTCCCG TTGCCCACCT TGGCGATGAA CTGAAGGAGT TCTCCGTGGG TAAAAGCATT 
GCACAGGGGA GCCCAGACTG GACAGATAGG CAGCCCTTTG AAGAGAAGGT TGACCTGGAA 
AATGTCACGA GAAGGAGGTT TTCGCTAAGA GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG 
ACGGGAACAG CAGCCGGGAA AACCGTCACC CAGACTGCAG CCCCCATGTC CTGGAAGCAG 
CATGATTCTG TAGAACCAGT ACAGGAGAAG TACCCCAGGA ATCCATGTGC CATGTTTGCT 
GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG GGCATTCTGG ATTCAGCCAG CAAAACCATG 
TCAATTAAAG AAAGATTAGC GCTGTTGAAG AAGAGTGGTG AAGAAGATTG GAAGAACAGA 
CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG TCTGTCTCCA GCAGCCTGCA CATACAAGAA 
GCGGAACAGT CCCTCAAGAA GAAGGAAGAA GGAGGATTTG CAGATGATAG TGCCATTTCT 
AATCTGCTTT GGGAACCTGT ATATGCTTCT ACTTATTCTC CTGCTATACC TGCTGCCCAT 
AAACACCTGT CTTTTGTATC TATTAATCAG CGGGTCACAG AAAGTCGAGA GAGCCAGATG 
ACTATCGAAG AGAGGAAGCA TCTCATCACT GTGAGAGAAG AAGCTTGGAA GACAAAAGGC 
AAAGGAGCGG CCAACGACTC CACCCAGTTT ACGGTGGCTG GCAGAATGGT GAAGAAAGGT 
TTGGCATCAC CCACCGCCAT AACTCCAGTA GCGTCTCCTA TTTGCAGTAA AACGAGGGGC 
ACCACACCTG TTTCTAAACC TTTGGAAGAT ATTGAAGCCA GACCAGACAT GCAGTTGGAA 
TCGGACCTGA AGTTGGACAG GCTGGAAACC TTTCTAAGAA GGCTGAATAA CAAAGTTGGT 
GGGATGCAGG AGACTGTACT CACTGTCACC GGTAAATCTG TTAAAGAGGT GATGAAGTTG 
GATGATGACG AGACCTTTGC CAGATTTTAC CGCAGCATGG ATTATACTGT GCCACGAAGC 
CCCGTGGAGC TAGACGAAGA TTTCGATGTC ATTTTCGATC CTTATGCCCC TAAGTTGACC 
TCCTCTGTGG CCGAGCACAA GCGCGCAGTT AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA 
AACCCCTTGA AAATGCTGGC AGCCAGAGAA GATCTCCTTC AGGAGTACAC GGAGCAGAGA 
CTAAACATTG CCTTCATGGA GTCAAAGCGG ATGAAAGTAG AAAAGATGTC CTCCAACTCC 
AACTTCTCAG AAGTCACTCT GGCAGGCTTA GCCAGTAAAG AAAACTTCAG CAACATCAGC 
CTGCGGAGCG TCAACCTGAC GGAACAGAAT TCCAACAATA GCGCGGTACC CTATAAGAAG 
CTGATGTTGT TACAGATTAA AGGAAGAAGA CACGTGCAGA CAAGACTGGT CGAACCTCGA 
GCTTCATCCC TCAACAGTGG GGACTGCTTC CTCCTGCTCT CCCCTCACTA CTGCTTCGTG 
TGGGTAGGAG AGTTTGCAAA TGTCATAGAA AAGGCAAAGG CCTCGGAACT TGCAAGTTTA 
ATTCAGACAA AGAGGGAACT TGGTTGTAGA GCTACATATG TCCAAACTAT CGAAGAAGGA 
ATTAATACAC ACACTCATGC GGCCAAAGAC TTCTGGAAGC TTCTGGGTGG CCAAACCAGT 
TACCAGTCTG CCGGAGACCC AAAAGAAGAT GAACTTTATG AAACAGCCAT AATTGAAACA 
AACTGCATTT ACCGTCTGAT GGATGACAAA CTGGTCCCTG AGGACGACTA CTGGGGAAAG 
ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA GAGGTACTGG TGTTTGATTT TGGTAGTGAA 
GTGTACGTGT GGCATGGAAA AGAAGTCACA TTAGCGCAAC GGAAAATAGC TTTTCAACTG 
GCAAAGCACT TATGGAACGG AACCTTTGAC TACGAGAACT GTGACATCAA CCCGTTGGAT 
CCTGGAGAAT GCAATCCGCT TATTCCCAGA AAAGGACAAG GGCGACCGGA TTGGGCAATT 
TTCGGGAGAC TTACAGAACA CAATGAGACG ATTTTGTTCA AAGAGAAATT TCTGGATTGG 
ACGGAACTGA AGAGGCCTAA TGAGAAGAGT GCCGGCGAAC TCACCCAGCA AAAGGAAGAT 
CCCAGGGCAG AAGCCAAGGC ATATGACGTG ACACGGATGG TGCCGGCACC CCAGGCCACA 
ACCGGCACTG TGCTGGATGG GGTGAATGTT GGTCGTGGCT ACGGCTTGGT GGATGGGGAT 
GACAGGAGGC AGTTTGAGAT CGCCAGTGTG TCTGTGGATG TGTGGCACAT CCTAGAATTT 
GACTACAGCA GGCTACCCAA ACAGAGCATC GGGCAGTTCC ACGAGGGAGA CGCCTATGTG 
GTCAAGTGGA AGTACATGGT GAGCACCGCA GTGGGAAGTC GACAAAAGGG AGAGCACCCA 
GTACGGGTAG CTGGCAAGGA GAAGTGTGTC TACTTCTTCT GGCAAGGCCG GCAGTCGACC 
GTGAGTGAGA AGGGCACATC AGCTCTGATG ACAGTGGAGC TGGATGAGGA GAGGGGTGCC 
CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA 
ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG GAGGAAGAAA ATGCACAAAG TGAGTGGAGG 
CTGTACTGTG TCCGAGGAGA AGTGCCCATG GAAGGAAATT TGCTGGAAGT GGCCTGTCAC 
TGTAGTAGCC TGAGGTCCAG GACTTCCATG GTTGTCCTCA ACATAAACAA AGCCCTCATT 
TACTTATGGC ACGGATGCAA AGCCCAAGCC CACACGAAGG AGGTTGGAAG GACTGCAGCA 
AATAAAATCA AAGAACAATG TCCCCTGGAA GCAGGACTGC ATAGTAGCAG CAAAGTGACA 
ATACACGAGT GTGACGAAGG ATCCGAGCCG CTGGGATTCT GGGATGCTTT AGGAAGGAGA 
GATAGAAAAG CGTACGACTG TATGCTTCAA GATCCTGGAA ATTTTAACTT CACACCTCGC 
CTATTCATCC TCAGCAGTTC CTCCGGTGAC TTCTTAGCCA CAGAGTTCAT ATATCCCGCC 
CGAGACCCCT CTGTGGTCAA CTCCATGCCT TTCCTGCAGG AAGATCTTTA CAGCGCGCCA 
CAGCCAGCAC TTTTCCTTGT TGACAATCAC CACGAGGTAT ACCTCTGGCA AGGCTGGTGG 
CCCATAGAGA ACAAGATCAC TGGATCGGCC CGCATTCGCT GGGCCTCCGA CCGGAAGAGC 
GCCATGGAAA CCGTGCTCCA GTACTGCAAA GGAAAAAATA TCAAGAGGCC GCCCCCCAAG 
TCTTACCTTA TCCATGCTGG TCTGGAGCCC TTGACATTCA CCAACATGTT CCCCAGCTGG 
GAGCACAGAG AGGACATTGC TGAAATCACA GAAATGGATA CCGAAGTTTC AAATCAGATC 
ACCCTTGTGG AAGATGTCTT AGCCAAGCTC TGTAAAACCA TCTATCCCCT GGCTGATCTG 
CTAGCCAGGC CGCTACCCGA GGGAGTTGAT CCTCTGAAGC TTGAGATTTA TCTCACCGAT 
GAAGACTTTG AGTTTGCACT AGACATGACA AGAGACGAAT ACAATGCTCT GCCCGCCTGG 
AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617051.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617108.1 Canis lupus familiaris supervillin (SVIL), transcript variant X7, mRNA.

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CloneID	OCe02725
Clone ID Related Accession (Same CDS sequence)	XM_005617051.2 , XM_005617051.3
Accession Version	XM_005617051.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	supervillin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726048.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617051.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACGT TGCAGGCATC TGCTCACCAC AAAGCCTTAG CCAGAGACCA GGCAAATGAG 
GGCAAAGATG CTGCTGAACA AGGTGAACCT GACTCCTCCA CTCTAAGCTT AGCAGAGAAG 
TTGGCCTTGT TTAACAAATT ATCCCAGCCA GTCTCAAAAG CTATTTCTAC CCGAAACAGA 
ATAGACATGA GACAGAGGAG AATGAATGCT CGCTATCAGA CTCAGCCAGT CACGCTTGGA 
GAGGTGGAAC AGGTGCAAAG TGGAAAGCTC ATTCCTTTCT CACCTACCGT TAACACATCT 
GTGTCCACCA TGGCATCTAC AGTCACTCCA ATGTATGCAG GGGATCTTCG GACAAAGTTG 
CCTGTGGATG ACAGAACAAG TGCCACTGAC CATAAATTTC CTTCTTTGAT GGAAAATTCA 
GATTCTCCGG TTAGAAGCAT TCTGAAGTCC CAAGTCTGGC AGCCTTTGAC TGAGGGCAGT 
GGAAACAGAG GAATGTTGAG AGAGCTGGGC GAGACAGAAG GGAAGAGAGT CTTGGCAGGT 
GGAGATGGTG GTAAAAAGTA CGGGTCCTTT GAGGAAGTGG AGCCACCCTG TCCTACCCTG 
AACAGAGTCT GGGAAGGAGA CAGCCATAAG GAAACCAAAT ATGCTGTTCC AAGAAAGGGT 
AGCCTGGAAC TAGCTCACCC TCCCGTTGCC CACCTTGGCG ATGAACTGAA GGAGTTCTCC 
GTGGGTAAAA GCATTGCACA GGGGAGCCCA GACTGGACAG ATAGGCAGCC CTTTGAAGAG 
AAGGTTGACC TGGAAAATGT CACGAGAAGG AGGTTTTCGC TAAGAGCAGA GTTTGGGGAG 
CCTGCTTCTG AGCAGACGGG AACAGCAGCC GGGAAAACCG TCACCCAGAC TGCAGCCCCC 
ATGTCCTGGA AGCAGCATGA TTCTGTAGAA CCAGTACAGG AGAAGTACCC CAGGAATCCA 
TGTGCCATGT TTGCTGCCGG AGAGATCAAA GTGCCAGCGG TGGAGGGCAT TCTGGATTCA 
GCCAGCAAAA CCATGTCAAT TAAAGAAAGA TTAGCGCTGT TGAAGAAGAG TGGTGAAGAA 
GATTGGAAGA ACAGACTGAG CAGAAAGCAG GAGTACGGCA AGGCGTCTGT CTCCAGCAGC 
CTGCACATAC AAGAAGCGGA ACAGTCCCTC AAGAAGAAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATCGAAGA GAGGAAGCAT CTCATCACTG TGAGAGAAGA AGCTTGGAAG 
ACAAAAGGCA AAGGAGCGGC CAACGACTCC ACCCAGTTTA CGGTGGCTGG CAGAATGGTG 
AAGAAAGGTT TGGCATCACC CACCGCCATA ACTCCAGTAG CGTCTCCTAT TTGCAGTAAA 
ACGAGGGGCA CCACACCTGT TTCTAAACCT TTGGAAGATA TTGAAGCCAG ACCAGACATG 
CAGTTGGAAT CGGACCTGAA GTTGGACAGG CTGGAAACCT TTCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAGGA GACTGTACTC ACTGTCACCG GTAAATCTGT TAAAGAGGTG 
ATGAAGTTGG ATGATGACGA GACCTTTGCC AGATTTTACC GCAGCATGGA TTATACTGTG 
CCACGAAGCC CCGTGGAGCT AGACGAAGAT TTCGATGTCA TTTTCGATCC TTATGCCCCT 
AAGTTGACCT CCTCTGTGGC CGAGCACAAG CGCGCAGTTA GGCCCAAGCG CCGGGTTCAG 
GCTTCCAGAA ACCCCTTGAA AATGCTGGCA GCCAGAGAAG ATCTCCTTCA GGAGTACACG 
GAGCAGAGAC TAAACATTGC CTTCATGGAG TCAAAGCGGA TGAAAGTAGA AAAGATGTCC 
TCCAACTCCA ACTTCTCAGA AGTCACTCTG GCAGGCTTAG CCAGTAAAGA AAACTTCAGC 
AACATCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAATAG CGCGGTACCC 
TATAAGAAGC TGATGTTGTT ACAGATTAAA GGAAGAAGAC ACGTGCAGAC AAGACTGGTC 
GAACCTCGAG CTTCATCCCT CAACAGTGGG GACTGCTTCC TCCTGCTCTC CCCTCACTAC 
TGCTTCGTGT GGGTAGGAGA GTTTGCAAAT GTCATAGAAA AGGCAAAGGC CTCGGAACTT 
GCAAGTTTAA TTCAGACAAA GAGGGAACTT GGTTGTAGAG CTACATATGT CCAAACTATC 
GAAGAAGGAA TTAATACACA CACTCATGCG GCCAAAGACT TCTGGAAGCT TCTGGGTGGC 
CAAACCAGTT ACCAGTCTGC CGGAGACCCA AAAGAAGATG AACTTTATGA AACAGCCATA 
ATTGAAACAA ACTGCATTTA CCGTCTGATG GATGACAAAC TGGTCCCTGA GGACGACTAC 
TGGGGAAAGA TTCCCAAGTG CTCCCTTCTG CAGTCAAAAG AGGTACTGGT GTTTGATTTT 
GGTAGTGAAG TGTACGTGTG GCATGGAAAA GAAGTCACAT TAGCGCAACG GAAAATAGCT 
TTTCAACTGG CAAAGCACTT ATGGAACGGA ACCTTTGACT ACGAGAACTG TGACATCAAC 
CCGTTGGATC CTGGAGAATG CAATCCGCTT ATTCCCAGAA AAGGACAAGG GCGACCGGAT 
TGGGCAATTT TCGGGAGACT TACAGAACAC AATGAGACGA TTTTGTTCAA AGAGAAATTT 
CTGGATTGGA CGGAACTGAA GAGGCCTAAT GAGAAGAGTG CCGGCGAACT CACCCAGCAA 
AAGGAAGATC CCAGGGCAGA AGCCAAGGCA TATGACGTGA CACGGATGGT GCCGGCACCC 
CAGGCCACAA CCGGCACTGT GCTGGATGGG GTGAATGTTG GTCGTGGCTA CGGCTTGGTG 
GATGGGGATG ACAGGAGGCA GTTTGAGATC GCCAGTGTGT CTGTGGATGT GTGGCACATC 
CTAGAATTTG ACTACAGCAG GCTACCCAAA CAGAGCATCG GGCAGTTCCA CGAGGGAGAC 
GCCTATGTGG TCAAGTGGAA GTACATGGTG AGCACCGCAG TGGGAAGTCG ACAAAAGGGA 
GAGCACCCAG TACGGGTAGC TGGCAAGGAG AAGTGTGTCT ACTTCTTCTG GCAAGGCCGG 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGATGAGGAG 
AGGGGTGCCC AGGTCCAGGT TCTGCAGGGA AAGGAGCCCC CCTGCTTTCT GCAGTGTTTC 
CAGGGGGGAA TGGTGGTACA CTCTGGGAGA CGGGAAGAGG AGGAAGAAAA TGCACAAAGT 
GAGTGGAGGC TGTACTGTGT CCGAGGAGAA GTGCCCATGG AAGGAAATTT GCTGGAAGTG 
GCCTGTCACT GTAGTAGCCT GAGGTCCAGG ACTTCCATGG TTGTCCTCAA CATAAACAAA 
GCCCTCATTT ACTTATGGCA CGGATGCAAA GCCCAAGCCC ACACGAAGGA GGTTGGAAGG 
ACTGCAGCAA ATAAAATCAA AGAACAATGT CCCCTGGAAG CAGGACTGCA TAGTAGCAGC 
AAAGTGACAA TACACGAGTG TGACGAAGGA TCCGAGCCGC TGGGATTCTG GGATGCTTTA 
GGAAGGAGAG ATAGAAAAGC GTACGACTGT ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCTCGCC TATTCATCCT CAGCAGTTCC TCCGGTGACT TCTTAGCCAC AGAGTTCATA 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCCATGCCTT TCCTGCAGGA AGATCTTTAC 
AGCGCGCCAC AGCCAGCACT TTTCCTTGTT GACAATCACC ACGAGGTATA CCTCTGGCAA 
GGCTGGTGGC CCATAGAGAA CAAGATCACT GGATCGGCCC GCATTCGCTG GGCCTCCGAC 
CGGAAGAGCG CCATGGAAAC CGTGCTCCAG TACTGCAAAG GAAAAAATAT CAAGAGGCCG 
CCCCCCAAGT CTTACCTTAT CCATGCTGGT CTGGAGCCCT TGACATTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA GGACATTGCT GAAATCACAG AAATGGATAC CGAAGTTTCA 
AATCAGATCA CCCTTGTGGA AGATGTCTTA GCCAAGCTCT GTAAAACCAT CTATCCCCTG 
GCTGATCTGC TAGCCAGGCC GCTACCCGAG GGAGTTGATC CTCTGAAGCT TGAGATTTAT 
CTCACCGATG AAGACTTTGA GTTTGCACTA GACATGACAA GAGACGAATA CAATGCTCTG 
CCCGCCTGGA AGCAGGTGAA CCTGAAGAAA GCAAAGGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617108.1
CDS	47..6814
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617051.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617051.2

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACGT TGCAGGCATC TGCTCACCAC AAAGCCTTAG CCAGAGACCA GGCAAATGAG 
GGCAAAGATG CTGCTGAACA AGGTGAACCT GACTCCTCCA CTCTAAGCTT AGCAGAGAAG 
TTGGCCTTGT TTAACAAATT ATCCCAGCCA GTCTCAAAAG CTATTTCTAC CCGAAACAGA 
ATAGACATGA GACAGAGGAG AATGAATGCT CGCTATCAGA CTCAGCCAGT CACGCTTGGA 
GAGGTGGAAC AGGTGCAAAG TGGAAAGCTC ATTCCTTTCT CACCTACCGT TAACACATCT 
GTGTCCACCA TGGCATCTAC AGTCACTCCA ATGTATGCAG GGGATCTTCG GACAAAGTTG 
CCTGTGGATG ACAGAACAAG TGCCACTGAC CATAAATTTC CTTCTTTGAT GGAAAATTCA 
GATTCTCCGG TTAGAAGCAT TCTGAAGTCC CAAGTCTGGC AGCCTTTGAC TGAGGGCAGT 
GGAAACAGAG GAATGTTGAG AGAGCTGGGC GAGACAGAAG GGAAGAGAGT CTTGGCAGGT 
GGAGATGGTG GTAAAAAGTA CGGGTCCTTT GAGGAAGTGG AGCCACCCTG TCCTACCCTG 
AACAGAGTCT GGGAAGGAGA CAGCCATAAG GAAACCAAAT ATGCTGTTCC AAGAAAGGGT 
AGCCTGGAAC TAGCTCACCC TCCCGTTGCC CACCTTGGCG ATGAACTGAA GGAGTTCTCC 
GTGGGTAAAA GCATTGCACA GGGGAGCCCA GACTGGACAG ATAGGCAGCC CTTTGAAGAG 
AAGGTTGACC TGGAAAATGT CACGAGAAGG AGGTTTTCGC TAAGAGCAGA GTTTGGGGAG 
CCTGCTTCTG AGCAGACGGG AACAGCAGCC GGGAAAACCG TCACCCAGAC TGCAGCCCCC 
ATGTCCTGGA AGCAGCATGA TTCTGTAGAA CCAGTACAGG AGAAGTACCC CAGGAATCCA 
TGTGCCATGT TTGCTGCCGG AGAGATCAAA GTGCCAGCGG TGGAGGGCAT TCTGGATTCA 
GCCAGCAAAA CCATGTCAAT TAAAGAAAGA TTAGCGCTGT TGAAGAAGAG TGGTGAAGAA 
GATTGGAAGA ACAGACTGAG CAGAAAGCAG GAGTACGGCA AGGCGTCTGT CTCCAGCAGC 
CTGCACATAC AAGAAGCGGA ACAGTCCCTC AAGAAGAAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATCGAAGA GAGGAAGCAT CTCATCACTG TGAGAGAAGA AGCTTGGAAG 
ACAAAAGGCA AAGGAGCGGC CAACGACTCC ACCCAGTTTA CGGTGGCTGG CAGAATGGTG 
AAGAAAGGTT TGGCATCACC CACCGCCATA ACTCCAGTAG CGTCTCCTAT TTGCAGTAAA 
ACGAGGGGCA CCACACCTGT TTCTAAACCT TTGGAAGATA TTGAAGCCAG ACCAGACATG 
CAGTTGGAAT CGGACCTGAA GTTGGACAGG CTGGAAACCT TTCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAGGA GACTGTACTC ACTGTCACCG GTAAATCTGT TAAAGAGGTG 
ATGAAGTTGG ATGATGACGA GACCTTTGCC AGATTTTACC GCAGCATGGA TTATACTGTG 
CCACGAAGCC CCGTGGAGCT AGACGAAGAT TTCGATGTCA TTTTCGATCC TTATGCCCCT 
AAGTTGACCT CCTCTGTGGC CGAGCACAAG CGCGCAGTTA GGCCCAAGCG CCGGGTTCAG 
GCTTCCAGAA ACCCCTTGAA AATGCTGGCA GCCAGAGAAG ATCTCCTTCA GGAGTACACG 
GAGCAGAGAC TAAACATTGC CTTCATGGAG TCAAAGCGGA TGAAAGTAGA AAAGATGTCC 
TCCAACTCCA ACTTCTCAGA AGTCACTCTG GCAGGCTTAG CCAGTAAAGA AAACTTCAGC 
AACATCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAATAG CGCGGTACCC 
TATAAGAAGC TGATGTTGTT ACAGATTAAA GGAAGAAGAC ACGTGCAGAC AAGACTGGTC 
GAACCTCGAG CTTCATCCCT CAACAGTGGG GACTGCTTCC TCCTGCTCTC CCCTCACTAC 
TGCTTCGTGT GGGTAGGAGA GTTTGCAAAT GTCATAGAAA AGGCAAAGGC CTCGGAACTT 
GCAAGTTTAA TTCAGACAAA GAGGGAACTT GGTTGTAGAG CTACATATGT CCAAACTATC 
GAAGAAGGAA TTAATACACA CACTCATGCG GCCAAAGACT TCTGGAAGCT TCTGGGTGGC 
CAAACCAGTT ACCAGTCTGC CGGAGACCCA AAAGAAGATG AACTTTATGA AACAGCCATA 
ATTGAAACAA ACTGCATTTA CCGTCTGATG GATGACAAAC TGGTCCCTGA GGACGACTAC 
TGGGGAAAGA TTCCCAAGTG CTCCCTTCTG CAGTCAAAAG AGGTACTGGT GTTTGATTTT 
GGTAGTGAAG TGTACGTGTG GCATGGAAAA GAAGTCACAT TAGCGCAACG GAAAATAGCT 
TTTCAACTGG CAAAGCACTT ATGGAACGGA ACCTTTGACT ACGAGAACTG TGACATCAAC 
CCGTTGGATC CTGGAGAATG CAATCCGCTT ATTCCCAGAA AAGGACAAGG GCGACCGGAT 
TGGGCAATTT TCGGGAGACT TACAGAACAC AATGAGACGA TTTTGTTCAA AGAGAAATTT 
CTGGATTGGA CGGAACTGAA GAGGCCTAAT GAGAAGAGTG CCGGCGAACT CACCCAGCAA 
AAGGAAGATC CCAGGGCAGA AGCCAAGGCA TATGACGTGA CACGGATGGT GCCGGCACCC 
CAGGCCACAA CCGGCACTGT GCTGGATGGG GTGAATGTTG GTCGTGGCTA CGGCTTGGTG 
GATGGGGATG ACAGGAGGCA GTTTGAGATC GCCAGTGTGT CTGTGGATGT GTGGCACATC 
CTAGAATTTG ACTACAGCAG GCTACCCAAA CAGAGCATCG GGCAGTTCCA CGAGGGAGAC 
GCCTATGTGG TCAAGTGGAA GTACATGGTG AGCACCGCAG TGGGAAGTCG ACAAAAGGGA 
GAGCACCCAG TACGGGTAGC TGGCAAGGAG AAGTGTGTCT ACTTCTTCTG GCAAGGCCGG 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGATGAGGAG 
AGGGGTGCCC AGGTCCAGGT TCTGCAGGGA AAGGAGCCCC CCTGCTTTCT GCAGTGTTTC 
CAGGGGGGAA TGGTGGTACA CTCTGGGAGA CGGGAAGAGG AGGAAGAAAA TGCACAAAGT 
GAGTGGAGGC TGTACTGTGT CCGAGGAGAA GTGCCCATGG AAGGAAATTT GCTGGAAGTG 
GCCTGTCACT GTAGTAGCCT GAGGTCCAGG ACTTCCATGG TTGTCCTCAA CATAAACAAA 
GCCCTCATTT ACTTATGGCA CGGATGCAAA GCCCAAGCCC ACACGAAGGA GGTTGGAAGG 
ACTGCAGCAA ATAAAATCAA AGAACAATGT CCCCTGGAAG CAGGACTGCA TAGTAGCAGC 
AAAGTGACAA TACACGAGTG TGACGAAGGA TCCGAGCCGC TGGGATTCTG GGATGCTTTA 
GGAAGGAGAG ATAGAAAAGC GTACGACTGT ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCTCGCC TATTCATCCT CAGCAGTTCC TCCGGTGACT TCTTAGCCAC AGAGTTCATA 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCCATGCCTT TCCTGCAGGA AGATCTTTAC 
AGCGCGCCAC AGCCAGCACT TTTCCTTGTT GACAATCACC ACGAGGTATA CCTCTGGCAA 
GGCTGGTGGC CCATAGAGAA CAAGATCACT GGATCGGCCC GCATTCGCTG GGCCTCCGAC 
CGGAAGAGCG CCATGGAAAC CGTGCTCCAG TACTGCAAAG GAAAAAATAT CAAGAGGCCG 
CCCCCCAAGT CTTACCTTAT CCATGCTGGT CTGGAGCCCT TGACATTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA GGACATTGCT GAAATCACAG AAATGGATAC CGAAGTTTCA 
AATCAGATCA CCCTTGTGGA AGATGTCTTA GCCAAGCTCT GTAAAACCAT CTATCCCCTG 
GCTGATCTGC TAGCCAGGCC GCTACCCGAG GGAGTTGATC CTCTGAAGCT TGAGATTTAT 
CTCACCGATG AAGACTTTGA GTTTGCACTA GACATGACAA GAGACGAATA CAATGCTCTG 
CCCGCCTGGA AGCAGGTGAA CCTGAAGAAA GCAAAGGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617051.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617108.1 Canis lupus familiaris supervillin (SVIL), transcript variant X8, mRNA.

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CloneID	OCe02725
Clone ID Related Accession (Same CDS sequence)	XM_005617051.2 , XM_005617051.3
Accession Version	XM_005617051.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617051.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACGT TGCAGGCATC TGCTCACCAC AAAGCCTTAG CCAGAGACCA GGCAAATGAG 
GGCAAAGATG CTGCTGAACA AGGTGAACCT GACTCCTCCA CTCTAAGCTT AGCAGAGAAG 
TTGGCCTTGT TTAACAAATT ATCCCAGCCA GTCTCAAAAG CTATTTCTAC CCGAAACAGA 
ATAGACATGA GACAGAGGAG AATGAATGCT CGCTATCAGA CTCAGCCAGT CACGCTTGGA 
GAGGTGGAAC AGGTGCAAAG TGGAAAGCTC ATTCCTTTCT CACCTACCGT TAACACATCT 
GTGTCCACCA TGGCATCTAC AGTCACTCCA ATGTATGCAG GGGATCTTCG GACAAAGTTG 
CCTGTGGATG ACAGAACAAG TGCCACTGAC CATAAATTTC CTTCTTTGAT GGAAAATTCA 
GATTCTCCGG TTAGAAGCAT TCTGAAGTCC CAAGTCTGGC AGCCTTTGAC TGAGGGCAGT 
GGAAACAGAG GAATGTTGAG AGAGCTGGGC GAGACAGAAG GGAAGAGAGT CTTGGCAGGT 
GGAGATGGTG GTAAAAAGTA CGGGTCCTTT GAGGAAGTGG AGCCACCCTG TCCTACCCTG 
AACAGAGTCT GGGAAGGAGA CAGCCATAAG GAAACCAAAT ATGCTGTTCC AAGAAAGGGT 
AGCCTGGAAC TAGCTCACCC TCCCGTTGCC CACCTTGGCG ATGAACTGAA GGAGTTCTCC 
GTGGGTAAAA GCATTGCACA GGGGAGCCCA GACTGGACAG ATAGGCAGCC CTTTGAAGAG 
AAGGTTGACC TGGAAAATGT CACGAGAAGG AGGTTTTCGC TAAGAGCAGA GTTTGGGGAG 
CCTGCTTCTG AGCAGACGGG AACAGCAGCC GGGAAAACCG TCACCCAGAC TGCAGCCCCC 
ATGTCCTGGA AGCAGCATGA TTCTGTAGAA CCAGTACAGG AGAAGTACCC CAGGAATCCA 
TGTGCCATGT TTGCTGCCGG AGAGATCAAA GTGCCAGCGG TGGAGGGCAT TCTGGATTCA 
GCCAGCAAAA CCATGTCAAT TAAAGAAAGA TTAGCGCTGT TGAAGAAGAG TGGTGAAGAA 
GATTGGAAGA ACAGACTGAG CAGAAAGCAG GAGTACGGCA AGGCGTCTGT CTCCAGCAGC 
CTGCACATAC AAGAAGCGGA ACAGTCCCTC AAGAAGAAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATCGAAGA GAGGAAGCAT CTCATCACTG TGAGAGAAGA AGCTTGGAAG 
ACAAAAGGCA AAGGAGCGGC CAACGACTCC ACCCAGTTTA CGGTGGCTGG CAGAATGGTG 
AAGAAAGGTT TGGCATCACC CACCGCCATA ACTCCAGTAG CGTCTCCTAT TTGCAGTAAA 
ACGAGGGGCA CCACACCTGT TTCTAAACCT TTGGAAGATA TTGAAGCCAG ACCAGACATG 
CAGTTGGAAT CGGACCTGAA GTTGGACAGG CTGGAAACCT TTCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAGGA GACTGTACTC ACTGTCACCG GTAAATCTGT TAAAGAGGTG 
ATGAAGTTGG ATGATGACGA GACCTTTGCC AGATTTTACC GCAGCATGGA TTATACTGTG 
CCACGAAGCC CCGTGGAGCT AGACGAAGAT TTCGATGTCA TTTTCGATCC TTATGCCCCT 
AAGTTGACCT CCTCTGTGGC CGAGCACAAG CGCGCAGTTA GGCCCAAGCG CCGGGTTCAG 
GCTTCCAGAA ACCCCTTGAA AATGCTGGCA GCCAGAGAAG ATCTCCTTCA GGAGTACACG 
GAGCAGAGAC TAAACATTGC CTTCATGGAG TCAAAGCGGA TGAAAGTAGA AAAGATGTCC 
TCCAACTCCA ACTTCTCAGA AGTCACTCTG GCAGGCTTAG CCAGTAAAGA AAACTTCAGC 
AACATCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAATAG CGCGGTACCC 
TATAAGAAGC TGATGTTGTT ACAGATTAAA GGAAGAAGAC ACGTGCAGAC AAGACTGGTC 
GAACCTCGAG CTTCATCCCT CAACAGTGGG GACTGCTTCC TCCTGCTCTC CCCTCACTAC 
TGCTTCGTGT GGGTAGGAGA GTTTGCAAAT GTCATAGAAA AGGCAAAGGC CTCGGAACTT 
GCAAGTTTAA TTCAGACAAA GAGGGAACTT GGTTGTAGAG CTACATATGT CCAAACTATC 
GAAGAAGGAA TTAATACACA CACTCATGCG GCCAAAGACT TCTGGAAGCT TCTGGGTGGC 
CAAACCAGTT ACCAGTCTGC CGGAGACCCA AAAGAAGATG AACTTTATGA AACAGCCATA 
ATTGAAACAA ACTGCATTTA CCGTCTGATG GATGACAAAC TGGTCCCTGA GGACGACTAC 
TGGGGAAAGA TTCCCAAGTG CTCCCTTCTG CAGTCAAAAG AGGTACTGGT GTTTGATTTT 
GGTAGTGAAG TGTACGTGTG GCATGGAAAA GAAGTCACAT TAGCGCAACG GAAAATAGCT 
TTTCAACTGG CAAAGCACTT ATGGAACGGA ACCTTTGACT ACGAGAACTG TGACATCAAC 
CCGTTGGATC CTGGAGAATG CAATCCGCTT ATTCCCAGAA AAGGACAAGG GCGACCGGAT 
TGGGCAATTT TCGGGAGACT TACAGAACAC AATGAGACGA TTTTGTTCAA AGAGAAATTT 
CTGGATTGGA CGGAACTGAA GAGGCCTAAT GAGAAGAGTG CCGGCGAACT CACCCAGCAA 
AAGGAAGATC CCAGGGCAGA AGCCAAGGCA TATGACGTGA CACGGATGGT GCCGGCACCC 
CAGGCCACAA CCGGCACTGT GCTGGATGGG GTGAATGTTG GTCGTGGCTA CGGCTTGGTG 
GATGGGGATG ACAGGAGGCA GTTTGAGATC GCCAGTGTGT CTGTGGATGT GTGGCACATC 
CTAGAATTTG ACTACAGCAG GCTACCCAAA CAGAGCATCG GGCAGTTCCA CGAGGGAGAC 
GCCTATGTGG TCAAGTGGAA GTACATGGTG AGCACCGCAG TGGGAAGTCG ACAAAAGGGA 
GAGCACCCAG TACGGGTAGC TGGCAAGGAG AAGTGTGTCT ACTTCTTCTG GCAAGGCCGG 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGATGAGGAG 
AGGGGTGCCC AGGTCCAGGT TCTGCAGGGA AAGGAGCCCC CCTGCTTTCT GCAGTGTTTC 
CAGGGGGGAA TGGTGGTACA CTCTGGGAGA CGGGAAGAGG AGGAAGAAAA TGCACAAAGT 
GAGTGGAGGC TGTACTGTGT CCGAGGAGAA GTGCCCATGG AAGGAAATTT GCTGGAAGTG 
GCCTGTCACT GTAGTAGCCT GAGGTCCAGG ACTTCCATGG TTGTCCTCAA CATAAACAAA 
GCCCTCATTT ACTTATGGCA CGGATGCAAA GCCCAAGCCC ACACGAAGGA GGTTGGAAGG 
ACTGCAGCAA ATAAAATCAA AGAACAATGT CCCCTGGAAG CAGGACTGCA TAGTAGCAGC 
AAAGTGACAA TACACGAGTG TGACGAAGGA TCCGAGCCGC TGGGATTCTG GGATGCTTTA 
GGAAGGAGAG ATAGAAAAGC GTACGACTGT ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCTCGCC TATTCATCCT CAGCAGTTCC TCCGGTGACT TCTTAGCCAC AGAGTTCATA 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCCATGCCTT TCCTGCAGGA AGATCTTTAC 
AGCGCGCCAC AGCCAGCACT TTTCCTTGTT GACAATCACC ACGAGGTATA CCTCTGGCAA 
GGCTGGTGGC CCATAGAGAA CAAGATCACT GGATCGGCCC GCATTCGCTG GGCCTCCGAC 
CGGAAGAGCG CCATGGAAAC CGTGCTCCAG TACTGCAAAG GAAAAAATAT CAAGAGGCCG 
CCCCCCAAGT CTTACCTTAT CCATGCTGGT CTGGAGCCCT TGACATTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA GGACATTGCT GAAATCACAG AAATGGATAC CGAAGTTTCA 
AATCAGATCA CCCTTGTGGA AGATGTCTTA GCCAAGCTCT GTAAAACCAT CTATCCCCTG 
GCTGATCTGC TAGCCAGGCC GCTACCCGAG GGAGTTGATC CTCTGAAGCT TGAGATTTAT 
CTCACCGATG AAGACTTTGA GTTTGCACTA GACATGACAA GAGACGAATA CAATGCTCTG 
CCCGCCTGGA AGCAGGTGAA CCTGAAGAAA GCAAAGGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617108.1
CDS	64..6831
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617051.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617051.3

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGTACC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCTCTT 
ATCAACTGGC CTTCCAGAGT TAAAGTTAGA GAAAAATTGG TGAGAGAGGA GAGTGCTCGC 
AGCAGCCCCG AAGTGGCCTC CGAGCCCTTC ACTCAGAGGA GACATCAGCC AGTGTCCGTC 
CATTGCTCGT CCTTTCAGTC AGAAAATTCG GCCTTTGATA GGGTCTCCAG CAAGGCGGCC 
AGCTCCGTGC GACAGCCCAT CCGCGGCTAT GTCCAACCGG CAGACCCCAT TCACACGGCC 
ACTCTGGTGA CCTCAGAGAT CCCTGAAAAC ATATCAGAAT ACAGCTGGGG GGGGTCGGCC 
ACCCAGAATG TCAGGAAGCC TCCCATCTCG AAGGTTCTAG AAAGTGAGAG AAGGGAGGAT 
ACTCCCATTC TCCATATTTG TGAGTCAAAA ACAGAGGAAG ATATGCTCTT CACCGAAGCT 
CTGGAGAAAA GCAGGAAAAC ACTTTTGGAT TTGGAGGACC ATGAGCTTAT GAGAAGCCAC 
GAAGAGCCCT CTGGAAATGA AGATTTGGGC AAGCCTGCTG TTAGCACAGT CACCTTAGAA 
CACCAGATGG AAGCAGAAAG TCTGCCACAC CCCCTTGAAA CTCAGCAACA ACCCACGGAG 
AGAATTAGCA GACAAGAGAT GGTTCTATAT GTTCAGAGCG AGCCCGTGTC CCAAGATGCC 
AGGGTGACAG GCCACAGAAG GGAAGCATTT ACAAAGAAGC GCAAGGTCCT CACCCGCTCC 
CTGTCTGACT ACACGGGCCC CCCTCAGCTG CAGGCCCCCC TCAAGTGTAA GGACTCCACT 
TCCAAGCAGG ATCCAGAGCT GCAGAGTTCC AGAGCCGAAG GGCCCCCCAC AGAAGTTGGC 
ATGCTGGACA CAAAGGTCTC AGTGGCCCAG CTGCGAAATG CATTTCTGGA GTCTGTCAAT 
GCCAGCAAGA AACGCGAACT CCCATCCCGG GTTGAGAGGT CAACCGAAGG AGTTGGCTTG 
CCTACCGGTG TGGAGCGGGA GAGAGGGTCC CGGAAACCAA GACGCTATTT TTCTCCTGGT 
GAAAGTAGAA AAACTTCTGA GAGATTTAGA ACTCAACCTA TAACCTCAGC AGAACGAAAG 
GAATCGGATA GGTCTGCTTC GCATTCAGAA ATGCCAACCA CTGAGGATGA AGAAAAGGTA 
GATGAACGAG CCAAGCTGAG TGTCGCTGCC AAAAGGCTGC TTTTCAGGGA AATGGAAAAA 
TCATTTGATG AGAAAAATGT TCCAAAGCGA CGCTCCAGAA ACGCGGCTGT GGAGCAAAGG 
CTGCGTCGGC TACAGGATAG ATCCCACACC CAGCCCGTTA CCACCGAGGA AGTGGCCATT 
GCAGCCACGT TGCAGGCATC TGCTCACCAC AAAGCCTTAG CCAGAGACCA GGCAAATGAG 
GGCAAAGATG CTGCTGAACA AGGTGAACCT GACTCCTCCA CTCTAAGCTT AGCAGAGAAG 
TTGGCCTTGT TTAACAAATT ATCCCAGCCA GTCTCAAAAG CTATTTCTAC CCGAAACAGA 
ATAGACATGA GACAGAGGAG AATGAATGCT CGCTATCAGA CTCAGCCAGT CACGCTTGGA 
GAGGTGGAAC AGGTGCAAAG TGGAAAGCTC ATTCCTTTCT CACCTACCGT TAACACATCT 
GTGTCCACCA TGGCATCTAC AGTCACTCCA ATGTATGCAG GGGATCTTCG GACAAAGTTG 
CCTGTGGATG ACAGAACAAG TGCCACTGAC CATAAATTTC CTTCTTTGAT GGAAAATTCA 
GATTCTCCGG TTAGAAGCAT TCTGAAGTCC CAAGTCTGGC AGCCTTTGAC TGAGGGCAGT 
GGAAACAGAG GAATGTTGAG AGAGCTGGGC GAGACAGAAG GGAAGAGAGT CTTGGCAGGT 
GGAGATGGTG GTAAAAAGTA CGGGTCCTTT GAGGAAGTGG AGCCACCCTG TCCTACCCTG 
AACAGAGTCT GGGAAGGAGA CAGCCATAAG GAAACCAAAT ATGCTGTTCC AAGAAAGGGT 
AGCCTGGAAC TAGCTCACCC TCCCGTTGCC CACCTTGGCG ATGAACTGAA GGAGTTCTCC 
GTGGGTAAAA GCATTGCACA GGGGAGCCCA GACTGGACAG ATAGGCAGCC CTTTGAAGAG 
AAGGTTGACC TGGAAAATGT CACGAGAAGG AGGTTTTCGC TAAGAGCAGA GTTTGGGGAG 
CCTGCTTCTG AGCAGACGGG AACAGCAGCC GGGAAAACCG TCACCCAGAC TGCAGCCCCC 
ATGTCCTGGA AGCAGCATGA TTCTGTAGAA CCAGTACAGG AGAAGTACCC CAGGAATCCA 
TGTGCCATGT TTGCTGCCGG AGAGATCAAA GTGCCAGCGG TGGAGGGCAT TCTGGATTCA 
GCCAGCAAAA CCATGTCAAT TAAAGAAAGA TTAGCGCTGT TGAAGAAGAG TGGTGAAGAA 
GATTGGAAGA ACAGACTGAG CAGAAAGCAG GAGTACGGCA AGGCGTCTGT CTCCAGCAGC 
CTGCACATAC AAGAAGCGGA ACAGTCCCTC AAGAAGAAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATCGAAGA GAGGAAGCAT CTCATCACTG TGAGAGAAGA AGCTTGGAAG 
ACAAAAGGCA AAGGAGCGGC CAACGACTCC ACCCAGTTTA CGGTGGCTGG CAGAATGGTG 
AAGAAAGGTT TGGCATCACC CACCGCCATA ACTCCAGTAG CGTCTCCTAT TTGCAGTAAA 
ACGAGGGGCA CCACACCTGT TTCTAAACCT TTGGAAGATA TTGAAGCCAG ACCAGACATG 
CAGTTGGAAT CGGACCTGAA GTTGGACAGG CTGGAAACCT TTCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAGGA GACTGTACTC ACTGTCACCG GTAAATCTGT TAAAGAGGTG 
ATGAAGTTGG ATGATGACGA GACCTTTGCC AGATTTTACC GCAGCATGGA TTATACTGTG 
CCACGAAGCC CCGTGGAGCT AGACGAAGAT TTCGATGTCA TTTTCGATCC TTATGCCCCT 
AAGTTGACCT CCTCTGTGGC CGAGCACAAG CGCGCAGTTA GGCCCAAGCG CCGGGTTCAG 
GCTTCCAGAA ACCCCTTGAA AATGCTGGCA GCCAGAGAAG ATCTCCTTCA GGAGTACACG 
GAGCAGAGAC TAAACATTGC CTTCATGGAG TCAAAGCGGA TGAAAGTAGA AAAGATGTCC 
TCCAACTCCA ACTTCTCAGA AGTCACTCTG GCAGGCTTAG CCAGTAAAGA AAACTTCAGC 
AACATCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAATAG CGCGGTACCC 
TATAAGAAGC TGATGTTGTT ACAGATTAAA GGAAGAAGAC ACGTGCAGAC AAGACTGGTC 
GAACCTCGAG CTTCATCCCT CAACAGTGGG GACTGCTTCC TCCTGCTCTC CCCTCACTAC 
TGCTTCGTGT GGGTAGGAGA GTTTGCAAAT GTCATAGAAA AGGCAAAGGC CTCGGAACTT 
GCAAGTTTAA TTCAGACAAA GAGGGAACTT GGTTGTAGAG CTACATATGT CCAAACTATC 
GAAGAAGGAA TTAATACACA CACTCATGCG GCCAAAGACT TCTGGAAGCT TCTGGGTGGC 
CAAACCAGTT ACCAGTCTGC CGGAGACCCA AAAGAAGATG AACTTTATGA AACAGCCATA 
ATTGAAACAA ACTGCATTTA CCGTCTGATG GATGACAAAC TGGTCCCTGA GGACGACTAC 
TGGGGAAAGA TTCCCAAGTG CTCCCTTCTG CAGTCAAAAG AGGTACTGGT GTTTGATTTT 
GGTAGTGAAG TGTACGTGTG GCATGGAAAA GAAGTCACAT TAGCGCAACG GAAAATAGCT 
TTTCAACTGG CAAAGCACTT ATGGAACGGA ACCTTTGACT ACGAGAACTG TGACATCAAC 
CCGTTGGATC CTGGAGAATG CAATCCGCTT ATTCCCAGAA AAGGACAAGG GCGACCGGAT 
TGGGCAATTT TCGGGAGACT TACAGAACAC AATGAGACGA TTTTGTTCAA AGAGAAATTT 
CTGGATTGGA CGGAACTGAA GAGGCCTAAT GAGAAGAGTG CCGGCGAACT CACCCAGCAA 
AAGGAAGATC CCAGGGCAGA AGCCAAGGCA TATGACGTGA CACGGATGGT GCCGGCACCC 
CAGGCCACAA CCGGCACTGT GCTGGATGGG GTGAATGTTG GTCGTGGCTA CGGCTTGGTG 
GATGGGGATG ACAGGAGGCA GTTTGAGATC GCCAGTGTGT CTGTGGATGT GTGGCACATC 
CTAGAATTTG ACTACAGCAG GCTACCCAAA CAGAGCATCG GGCAGTTCCA CGAGGGAGAC 
GCCTATGTGG TCAAGTGGAA GTACATGGTG AGCACCGCAG TGGGAAGTCG ACAAAAGGGA 
GAGCACCCAG TACGGGTAGC TGGCAAGGAG AAGTGTGTCT ACTTCTTCTG GCAAGGCCGG 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGATGAGGAG 
AGGGGTGCCC AGGTCCAGGT TCTGCAGGGA AAGGAGCCCC CCTGCTTTCT GCAGTGTTTC 
CAGGGGGGAA TGGTGGTACA CTCTGGGAGA CGGGAAGAGG AGGAAGAAAA TGCACAAAGT 
GAGTGGAGGC TGTACTGTGT CCGAGGAGAA GTGCCCATGG AAGGAAATTT GCTGGAAGTG 
GCCTGTCACT GTAGTAGCCT GAGGTCCAGG ACTTCCATGG TTGTCCTCAA CATAAACAAA 
GCCCTCATTT ACTTATGGCA CGGATGCAAA GCCCAAGCCC ACACGAAGGA GGTTGGAAGG 
ACTGCAGCAA ATAAAATCAA AGAACAATGT CCCCTGGAAG CAGGACTGCA TAGTAGCAGC 
AAAGTGACAA TACACGAGTG TGACGAAGGA TCCGAGCCGC TGGGATTCTG GGATGCTTTA 
GGAAGGAGAG ATAGAAAAGC GTACGACTGT ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCTCGCC TATTCATCCT CAGCAGTTCC TCCGGTGACT TCTTAGCCAC AGAGTTCATA 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCCATGCCTT TCCTGCAGGA AGATCTTTAC 
AGCGCGCCAC AGCCAGCACT TTTCCTTGTT GACAATCACC ACGAGGTATA CCTCTGGCAA 
GGCTGGTGGC CCATAGAGAA CAAGATCACT GGATCGGCCC GCATTCGCTG GGCCTCCGAC 
CGGAAGAGCG CCATGGAAAC CGTGCTCCAG TACTGCAAAG GAAAAAATAT CAAGAGGCCG 
CCCCCCAAGT CTTACCTTAT CCATGCTGGT CTGGAGCCCT TGACATTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA GGACATTGCT GAAATCACAG AAATGGATAC CGAAGTTTCA 
AATCAGATCA CCCTTGTGGA AGATGTCTTA GCCAAGCTCT GTAAAACCAT CTATCCCCTG 
GCTGATCTGC TAGCCAGGCC GCTACCCGAG GGAGTTGATC CTCTGAAGCT TGAGATTTAT 
CTCACCGATG AAGACTTTGA GTTTGCACTA GACATGACAA GAGACGAATA CAATGCTCTG 
CCCGCCTGGA AGCAGGTGAA CCTGAAGAAA GCAAAGGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617052.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617109.1 Canis lupus familiaris supervillin (SVIL), transcript variant X9, mRNA.

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CloneID	OCe02726
Clone ID Related Accession (Same CDS sequence)	XM_005617052.3 , XM_014122029.2 , XM_005617053.2 , XM_014122029.1 , XM_014122047.1 , XM_005617052.2 , XM_022407064.1 , XM_022407070.1 , XM_014122047.2 , XM_005617053.3 , XM_022407072.1
Accession Version	XM_005617052.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617052.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617109.1
CDS	434..7162
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617052.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617052.3

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_014122029.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977504.1 Canis lupus familiaris supervillin (SVIL), transcript variant X12, mRNA.

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CloneID	OCe02726
Clone ID Related Accession (Same CDS sequence)	XM_005617052.3 , XM_014122029.2 , XM_005617053.2 , XM_014122029.1 , XM_014122047.1 , XM_005617052.2 , XM_022407064.1 , XM_022407070.1 , XM_014122047.2 , XM_005617053.3 , XM_022407072.1
Accession Version	XM_014122029.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977504.1
CDS	742..7470
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_014122029.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122029.2

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617053.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617110.1 Canis lupus familiaris supervillin (SVIL), transcript variant X10, mRNA.

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CloneID	OCe02726
Clone ID Related Accession (Same CDS sequence)	XM_005617052.3 , XM_014122029.2 , XM_005617053.2 , XM_014122029.1 , XM_014122047.1 , XM_005617052.2 , XM_022407064.1 , XM_022407070.1 , XM_014122047.2 , XM_005617053.3 , XM_022407072.1
Accession Version	XM_005617053.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	supervillin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726048.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617053.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617110.1
CDS	381..7109
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617053.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617053.2

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_014122029.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977504.1 Canis lupus familiaris supervillin (SVIL), transcript variant X8, mRNA.

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CloneID	OCe02726
Clone ID Related Accession (Same CDS sequence)	XM_005617052.3 , XM_014122029.2 , XM_005617053.2 , XM_014122029.1 , XM_014122047.1 , XM_005617052.2 , XM_022407064.1 , XM_022407070.1 , XM_014122047.2 , XM_005617053.3 , XM_022407072.1
Accession Version	XM_014122029.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	supervillin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726048.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977504.1
CDS	746..7474
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_014122029.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122029.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_014122047.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977522.1 Canis lupus familiaris supervillin (SVIL), transcript variant X11, mRNA.

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CloneID	OCe02726
Clone ID Related Accession (Same CDS sequence)	XM_005617052.3 , XM_014122029.2 , XM_005617053.2 , XM_014122029.1 , XM_014122047.1 , XM_005617052.2 , XM_022407064.1 , XM_022407070.1 , XM_014122047.2 , XM_005617053.3 , XM_022407072.1
Accession Version	XM_014122047.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	supervillin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726048.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977522.1
CDS	292..7020
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_014122047.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122047.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617052.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617109.1 Canis lupus familiaris supervillin (SVIL), transcript variant X9, mRNA.

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CloneID	OCe02726
Clone ID Related Accession (Same CDS sequence)	XM_005617052.3 , XM_014122029.2 , XM_005617053.2 , XM_014122029.1 , XM_014122047.1 , XM_005617052.2 , XM_022407064.1 , XM_022407070.1 , XM_014122047.2 , XM_005617053.3 , XM_022407072.1
Accession Version	XM_005617052.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	supervillin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726048.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617052.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617109.1
CDS	428..7156
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617052.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617052.2

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_022407064.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262772.1 Canis lupus familiaris supervillin (SVIL), transcript variant X11, mRNA.

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CloneID	OCe02726
Clone ID Related Accession (Same CDS sequence)	XM_005617052.3 , XM_014122029.2 , XM_005617053.2 , XM_014122029.1 , XM_014122047.1 , XM_005617052.2 , XM_022407064.1 , XM_022407070.1 , XM_014122047.2 , XM_005617053.3 , XM_022407072.1
Accession Version	XM_022407064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262772.1
CDS	322..7050
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_022407064.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022407064.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_022407070.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262778.1 Canis lupus familiaris supervillin (SVIL), transcript variant X13, mRNA.

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CloneID	OCe02726
Clone ID Related Accession (Same CDS sequence)	XM_005617052.3 , XM_014122029.2 , XM_005617053.2 , XM_014122029.1 , XM_014122047.1 , XM_005617052.2 , XM_022407064.1 , XM_022407070.1 , XM_014122047.2 , XM_005617053.3 , XM_022407072.1
Accession Version	XM_022407070.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262778.1
CDS	240..6968
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_022407070.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022407070.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_014122047.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977522.1 Canis lupus familiaris supervillin (SVIL), transcript variant X14, mRNA.

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CloneID	OCe02726
Clone ID Related Accession (Same CDS sequence)	XM_005617052.3 , XM_014122029.2 , XM_005617053.2 , XM_014122029.1 , XM_014122047.1 , XM_005617052.2 , XM_022407064.1 , XM_022407070.1 , XM_014122047.2 , XM_005617053.3 , XM_022407072.1
Accession Version	XM_014122047.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122047.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977522.1
CDS	275..7003
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_014122047.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122047.2

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005617053.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617110.1 Canis lupus familiaris supervillin (SVIL), transcript variant X10, mRNA.

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CloneID	OCe02726
Clone ID Related Accession (Same CDS sequence)	XM_005617052.3 , XM_014122029.2 , XM_005617053.2 , XM_014122029.1 , XM_014122047.1 , XM_005617052.2 , XM_022407064.1 , XM_022407070.1 , XM_014122047.2 , XM_005617053.3 , XM_022407072.1
Accession Version	XM_005617053.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617053.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617110.1
CDS	432..7160
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005617053.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617053.3

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_022407072.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262780.1 Canis lupus familiaris supervillin (SVIL), transcript variant X15, mRNA.

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CloneID	OCe02726
Clone ID Related Accession (Same CDS sequence)	XM_005617052.3 , XM_014122029.2 , XM_005617053.2 , XM_014122029.1 , XM_014122047.1 , XM_005617052.2 , XM_022407064.1 , XM_022407070.1 , XM_014122047.2 , XM_005617053.3 , XM_022407072.1
Accession Version	XM_022407072.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262780.1
CDS	229..6957
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARASTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_022407072.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022407072.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTAGAAGGAA TCGAGACGGA CACCCAGCCC 
ATCCTCTTAC AGAGCTGCAC GGGGTTAGTG ACTCATCGCC TGTTGGAGGA AGACACACCC 
AGATACATGC GAGCCACAGA CCCTGCCAGC CCCCACACTG GCCGGTCCAA TGAAGACGAA 
GACACCTCGG ATTCCTCGGC GGAAAAGCAG ACCCGCTCCA GGCTCTGCAC GCCAAGCTCG 
GGCGTCCACG GTGAGCCGTC GGGCGGTCCA GCTGCCCCGG ACACGCAGGC CCTGGAGTCC 
AAGGCCGAGA GAATCGCGCG CTACAAGGCG GAAAGGCGCC GGCAGCTGGC CGAGAAGTAC 
GGCATAGCCC TGGATCCCGA AGCCGACGCG GAATCGCAGT CCCGATACTC CAAGTCCAGG 
AAGGACGCGG AGGCTGCGGA CAAAAGGGCT GGGAAGGGCG ACAGGCAGGC CGAGCCGGGC 
CCGGAGGCGA GCTCTGCGCA CCCCAGGCCC TGCGTGCGCG AGCCCAGGGA CCACGGCCTC 
CTCGCGGGCG ATGGTCTGTC CGACCCCGAC GTGCTGCTCG GCGCGGAGAA CCACAGACGT 
GCACAGGAGC CCGGCGCCGG CGGGCCGCCC CGTGACCCGG CGCCCCCGGC CGAGACTGCC 
TCGCCCTGCC CGTTCCCCGG GCGAGAAGGC GCCTTCAGTG AAGTGCCGAG GTCCCCGAGG 
TCCCCGAGGT CCCCGAGGCC CACGCGCAGC GCGGCCCCGG CCTCCCTGGG CCCCTCGGCC 
GCTGACCCGC CGCTGGCTCC CGAGGCCCGA GCCAGTACCG GGAAACCCAA ACATGAGTGG 
TTTCTCCAGA AAGATTCCGA AGGGGACACA CCTTCTCTTA TCAACTGGCC TTCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_022407049.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262757.1 Canis lupus familiaris supervillin (SVIL), transcript variant X4, mRNA.

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CloneID	OCe286614
Clone ID Related Accession (Same CDS sequence)	XM_022407049.1
Accession Version	XM_022407049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	supervillin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262757.1
CDS	65..6973
Translation	MFRSPEIASYLGAFKFSEKQDFNQKNLSFIGLPRQRKVPSFQKEFWLEDKEQLANHKRGIDAKLLTALPKVAELRKIFEPKRNEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHTGRSNEDEDTSDSSAEKQTRSRLCTPSSGVHGEPSGGPAAPDTQALESKAERIARYKAERRRQLAEKYGIALDPEADAESQSRYSKSRKDAEAADKRAGKGDRQAEPGPEASSAHPRPCVREPRDHGLLAGDGLSDPDVLLGAENHRRAQEPGAGGPPRDPAPPAETASPCPFPGREGAFSEVPRSPRSPRSPRPTRSAAPASLGPSAADPPLAPEARARVKVREKLVREESARSSPEVASEPFTQRRHQPVSVHCSSFQSENSAFDRVSSKAASSVRQPIRGYVQPADPIHTATLVTSEIPENISEYSWGGSATQNVRKPPISKVLESERREDTPILHICESKTEEDMLFTEALEKSRKTLLDLEDHELMRSHEEPSGNEDLGKPAVSTVTLEHQMEAESLPHPLETQQQPTERISRQEMVLYVQSEPVSQDARVTGHRREAFTKKRKVLTRSLSDYTGPPQLQAPLKCKDSTSKQDPELQSSRAEGPPTEVGMLDTKVSVAQLRNAFLESVNASKKRELPSRVERSTEGVGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRSASHSEMPTTEDEEKVDERAKLSVAAKRLLFREMEKSFDEKNVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVAIAATEPIPASCSVATHPVMARLPSPTVAKSTVQPARLQASAHHKALARDQANEGKDAAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDMRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPTVNTSVSTMASTVTPMYAGDLRTKLPVDDRTSATDHKFPSLMENSDSPVRSILKSQVWQPLTEGSGNRGMLRELGETEGKRVLAGGDGGKKYGSFEEVEPPCPTLNRVWEGDSHKETKYAVPRKGSLELAHPPVAHLGDELKEFSVGKSIAQGSPDWTDRQPFEEKVDLENVTRRRFSLRAEFGEPASEQTGTAAGKTVTQTAAPMSWKQHDSVEPVQEKYPRNPCAMFAAGEIKVPAVEGILDSASKTMSIKERLALLKKSGEEDWKNRLSRKQEYGKASVSSSLHIQEAEQSLKKKEEGGFADDSAISNLLWEPVYASTYSPAIPAAHKHLSFVSINQRVTESRESQMTIEERKHLITVREEAWKTKGKGAANDSTQFTVAGRMVKKGLASPTAITPVASPICSKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKLDDDETFARFYRSMDYTVPRSPVELDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASRNPLKMLAAREDLLQEYTEQRLNIAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNISLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLSPHYCFVWVGEFANVIEKAKASELASLIQTKRELGCRATYVQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPEDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKSAGELTQQKEDPRAEAKAYDVTRMVPAPQATTGTVLDGVNVGRGYGLVDGDDRRQFEIASVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYMVSTAVGSRQKGEHPVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENAQSEWRLYCVRGEVPMEGNLLEVACHCSSLRSRTSMVVLNINKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFLATEFIYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNIKRPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_022407049.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris supervillin (SVIL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022407049.1

ORF Insert Sequence:

ATGTTCAGAA GCCCAGAGAT TGCCAGCTAT CTGGGAGCTT TCAAGTTCTC TGAGAAACAA 
GATTTCAACC AGAAAAACTT AAGCTTCATT GGATTACCAC GTCAAAGAAA AGTTCCAAGC 
TTTCAGAAAG AGTTCTGGCT TGAAGATAAA GAACAGCTTG CTAACCACAA AAGAGGAATC 
GATGCTAAGC TTTTAACTGC ACTTCCTAAA GTTGCAGAAT TAAGAAAAAT CTTTGAACCA 
AAGAGAAACG AATTTTTAGA AATGAAAAGA AAAGAAAGAA TTGCCAGGCG CCTAGAAGGA 
ATCGAGACGG ACACCCAGCC CATCCTCTTA CAGAGCTGCA CGGGGTTAGT GACTCATCGC 
CTGTTGGAGG AAGACACACC CAGATACATG CGAGCCACAG ACCCTGCCAG CCCCCACACT 
GGCCGGTCCA ATGAAGACGA AGACACCTCG GATTCCTCGG CGGAAAAGCA GACCCGCTCC 
AGGCTCTGCA CGCCAAGCTC GGGCGTCCAC GGTGAGCCGT CGGGCGGTCC AGCTGCCCCG 
GACACGCAGG CCCTGGAGTC CAAGGCCGAG AGAATCGCGC GCTACAAGGC GGAAAGGCGC 
CGGCAGCTGG CCGAGAAGTA CGGCATAGCC CTGGATCCCG AAGCCGACGC GGAATCGCAG 
TCCCGATACT CCAAGTCCAG GAAGGACGCG GAGGCTGCGG ACAAAAGGGC TGGGAAGGGC 
GACAGGCAGG CCGAGCCGGG CCCGGAGGCG AGCTCTGCGC ACCCCAGGCC CTGCGTGCGC 
GAGCCCAGGG ACCACGGCCT CCTCGCGGGC GATGGTCTGT CCGACCCCGA CGTGCTGCTC 
GGCGCGGAGA ACCACAGACG TGCACAGGAG CCCGGCGCCG GCGGGCCGCC CCGTGACCCG 
GCGCCCCCGG CCGAGACTGC CTCGCCCTGC CCGTTCCCCG GGCGAGAAGG CGCCTTCAGT 
GAAGTGCCGA GGTCCCCGAG GTCCCCGAGG TCCCCGAGGC CCACGCGCAG CGCGGCCCCG 
GCCTCCCTGG GCCCCTCGGC CGCTGACCCG CCGCTGGCTC CCGAGGCCCG AGCCAGAGTT 
AAAGTTAGAG AAAAATTGGT GAGAGAGGAG AGTGCTCGCA GCAGCCCCGA AGTGGCCTCC 
GAGCCCTTCA CTCAGAGGAG ACATCAGCCA GTGTCCGTCC ATTGCTCGTC CTTTCAGTCA 
GAAAATTCGG CCTTTGATAG GGTCTCCAGC AAGGCGGCCA GCTCCGTGCG ACAGCCCATC 
CGCGGCTATG TCCAACCGGC AGACCCCATT CACACGGCCA CTCTGGTGAC CTCAGAGATC 
CCTGAAAACA TATCAGAATA CAGCTGGGGG GGGTCGGCCA CCCAGAATGT CAGGAAGCCT 
CCCATCTCGA AGGTTCTAGA AAGTGAGAGA AGGGAGGATA CTCCCATTCT CCATATTTGT 
GAGTCAAAAA CAGAGGAAGA TATGCTCTTC ACCGAAGCTC TGGAGAAAAG CAGGAAAACA 
CTTTTGGATT TGGAGGACCA TGAGCTTATG AGAAGCCACG AAGAGCCCTC TGGAAATGAA 
GATTTGGGCA AGCCTGCTGT TAGCACAGTC ACCTTAGAAC ACCAGATGGA AGCAGAAAGT 
CTGCCACACC CCCTTGAAAC TCAGCAACAA CCCACGGAGA GAATTAGCAG ACAAGAGATG 
GTTCTATATG TTCAGAGCGA GCCCGTGTCC CAAGATGCCA GGGTGACAGG CCACAGAAGG 
GAAGCATTTA CAAAGAAGCG CAAGGTCCTC ACCCGCTCCC TGTCTGACTA CACGGGCCCC 
CCTCAGCTGC AGGCCCCCCT CAAGTGTAAG GACTCCACTT CCAAGCAGGA TCCAGAGCTG 
CAGAGTTCCA GAGCCGAAGG GCCCCCCACA GAAGTTGGCA TGCTGGACAC AAAGGTCTCA 
GTGGCCCAGC TGCGAAATGC ATTTCTGGAG TCTGTCAATG CCAGCAAGAA ACGCGAACTC 
CCATCCCGGG TTGAGAGGTC AACCGAAGGA GTTGGCTTGC CTACCGGTGT GGAGCGGGAG 
AGAGGGTCCC GGAAACCAAG ACGCTATTTT TCTCCTGGTG AAAGTAGAAA AACTTCTGAG 
AGATTTAGAA CTCAACCTAT AACCTCAGCA GAACGAAAGG AATCGGATAG GTCTGCTTCG 
CATTCAGAAA TGCCAACCAC TGAGGATGAA GAAAAGGTAG ATGAACGAGC CAAGCTGAGT 
GTCGCTGCCA AAAGGCTGCT TTTCAGGGAA ATGGAAAAAT CATTTGATGA GAAAAATGTT 
CCAAAGCGAC GCTCCAGAAA CGCGGCTGTG GAGCAAAGGC TGCGTCGGCT ACAGGATAGA 
TCCCACACCC AGCCCGTTAC CACCGAGGAA GTGGCCATTG CAGCCACTGA ACCTATCCCT 
GCTTCGTGCT CTGTGGCCAC CCACCCTGTA ATGGCAAGAC TTCCTAGTCC CACTGTAGCT 
AAGAGCACTG TGCAGCCTGC CAGGTTGCAG GCATCTGCTC ACCACAAAGC CTTAGCCAGA 
GACCAGGCAA ATGAGGGCAA AGATGCTGCT GAACAAGGTG AACCTGACTC CTCCACTCTA 
AGCTTAGCAG AGAAGTTGGC CTTGTTTAAC AAATTATCCC AGCCAGTCTC AAAAGCTATT 
TCTACCCGAA ACAGAATAGA CATGAGACAG AGGAGAATGA ATGCTCGCTA TCAGACTCAG 
CCAGTCACGC TTGGAGAGGT GGAACAGGTG CAAAGTGGAA AGCTCATTCC TTTCTCACCT 
ACCGTTAACA CATCTGTGTC CACCATGGCA TCTACAGTCA CTCCAATGTA TGCAGGGGAT 
CTTCGGACAA AGTTGCCTGT GGATGACAGA ACAAGTGCCA CTGACCATAA ATTTCCTTCT 
TTGATGGAAA ATTCAGATTC TCCGGTTAGA AGCATTCTGA AGTCCCAAGT CTGGCAGCCT 
TTGACTGAGG GCAGTGGAAA CAGAGGAATG TTGAGAGAGC TGGGCGAGAC AGAAGGGAAG 
AGAGTCTTGG CAGGTGGAGA TGGTGGTAAA AAGTACGGGT CCTTTGAGGA AGTGGAGCCA 
CCCTGTCCTA CCCTGAACAG AGTCTGGGAA GGAGACAGCC ATAAGGAAAC CAAATATGCT 
GTTCCAAGAA AGGGTAGCCT GGAACTAGCT CACCCTCCCG TTGCCCACCT TGGCGATGAA 
CTGAAGGAGT TCTCCGTGGG TAAAAGCATT GCACAGGGGA GCCCAGACTG GACAGATAGG 
CAGCCCTTTG AAGAGAAGGT TGACCTGGAA AATGTCACGA GAAGGAGGTT TTCGCTAAGA 
GCAGAGTTTG GGGAGCCTGC TTCTGAGCAG ACGGGAACAG CAGCCGGGAA AACCGTCACC 
CAGACTGCAG CCCCCATGTC CTGGAAGCAG CATGATTCTG TAGAACCAGT ACAGGAGAAG 
TACCCCAGGA ATCCATGTGC CATGTTTGCT GCCGGAGAGA TCAAAGTGCC AGCGGTGGAG 
GGCATTCTGG ATTCAGCCAG CAAAACCATG TCAATTAAAG AAAGATTAGC GCTGTTGAAG 
AAGAGTGGTG AAGAAGATTG GAAGAACAGA CTGAGCAGAA AGCAGGAGTA CGGCAAGGCG 
TCTGTCTCCA GCAGCCTGCA CATACAAGAA GCGGAACAGT CCCTCAAGAA GAAGGAAGAA 
GGAGGATTTG CAGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATACC TGCTGCCCAT AAACACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATCGAAG AGAGGAAGCA TCTCATCACT 
GTGAGAGAAG AAGCTTGGAA GACAAAAGGC AAAGGAGCGG CCAACGACTC CACCCAGTTT 
ACGGTGGCTG GCAGAATGGT GAAGAAAGGT TTGGCATCAC CCACCGCCAT AACTCCAGTA 
GCGTCTCCTA TTTGCAGTAA AACGAGGGGC ACCACACCTG TTTCTAAACC TTTGGAAGAT 
ATTGAAGCCA GACCAGACAT GCAGTTGGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAGG AGACTGTACT CACTGTCACC 
GGTAAATCTG TTAAAGAGGT GATGAAGTTG GATGATGACG AGACCTTTGC CAGATTTTAC 
CGCAGCATGG ATTATACTGT GCCACGAAGC CCCGTGGAGC TAGACGAAGA TTTCGATGTC 
ATTTTCGATC CTTATGCCCC TAAGTTGACC TCCTCTGTGG CCGAGCACAA GCGCGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCTTCCAGA AACCCCTTGA AAATGCTGGC AGCCAGAGAA 
GATCTCCTTC AGGAGTACAC GGAGCAGAGA CTAAACATTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC CTCCAACTCC AACTTCTCAG AAGTCACTCT GGCAGGCTTA 
GCCAGTAAAG AAAACTTCAG CAACATCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAATA GCGCGGTACC CTATAAGAAG CTGATGTTGT TACAGATTAA AGGAAGAAGA 
CACGTGCAGA CAAGACTGGT CGAACCTCGA GCTTCATCCC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CCCCTCACTA CTGCTTCGTG TGGGTAGGAG AGTTTGCAAA TGTCATAGAA 
AAGGCAAAGG CCTCGGAACT TGCAAGTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACATATG TCCAAACTAT CGAAGAAGGA ATTAATACAC ACACTCATGC GGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAGTCTG CCGGAGACCC AAAAGAAGAT 
GAACTTTATG AAACAGCCAT AATTGAAACA AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTGGTCCCTG AGGACGACTA CTGGGGAAAG ATTCCCAAGT GCTCCCTTCT GCAGTCAAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTGTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCGCAAC GGAAAATAGC TTTTCAACTG GCAAAGCACT TATGGAACGG AACCTTTGAC 
TACGAGAACT GTGACATCAA CCCGTTGGAT CCTGGAGAAT GCAATCCGCT TATTCCCAGA 
AAAGGACAAG GGCGACCGGA TTGGGCAATT TTCGGGAGAC TTACAGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAATT TCTGGATTGG ACGGAACTGA AGAGGCCTAA TGAGAAGAGT 
GCCGGCGAAC TCACCCAGCA AAAGGAAGAT CCCAGGGCAG AAGCCAAGGC ATATGACGTG 
ACACGGATGG TGCCGGCACC CCAGGCCACA ACCGGCACTG TGCTGGATGG GGTGAATGTT 
GGTCGTGGCT ACGGCTTGGT GGATGGGGAT GACAGGAGGC AGTTTGAGAT CGCCAGTGTG 
TCTGTGGATG TGTGGCACAT CCTAGAATTT GACTACAGCA GGCTACCCAA ACAGAGCATC 
GGGCAGTTCC ACGAGGGAGA CGCCTATGTG GTCAAGTGGA AGTACATGGT GAGCACCGCA 
GTGGGAAGTC GACAAAAGGG AGAGCACCCA GTACGGGTAG CTGGCAAGGA GAAGTGTGTC 
TACTTCTTCT GGCAAGGCCG GCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGATGAGGA GAGGGGTGCC CAGGTCCAGG TTCTGCAGGG AAAGGAGCCC 
CCCTGCTTTC TGCAGTGTTT CCAGGGGGGA ATGGTGGTAC ACTCTGGGAG ACGGGAAGAG 
GAGGAAGAAA ATGCACAAAG TGAGTGGAGG CTGTACTGTG TCCGAGGAGA AGTGCCCATG 
GAAGGAAATT TGCTGGAAGT GGCCTGTCAC TGTAGTAGCC TGAGGTCCAG GACTTCCATG 
GTTGTCCTCA ACATAAACAA AGCCCTCATT TACTTATGGC ACGGATGCAA AGCCCAAGCC 
CACACGAAGG AGGTTGGAAG GACTGCAGCA AATAAAATCA AAGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTGACA ATACACGAGT GTGACGAAGG ATCCGAGCCG 
CTGGGATTCT GGGATGCTTT AGGAAGGAGA GATAGAAAAG CGTACGACTG TATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCTCGC CTATTCATCC TCAGCAGTTC CTCCGGTGAC 
TTCTTAGCCA CAGAGTTCAT ATATCCCGCC CGAGACCCCT CTGTGGTCAA CTCCATGCCT 
TTCCTGCAGG AAGATCTTTA CAGCGCGCCA CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTAT ACCTCTGGCA AGGCTGGTGG CCCATAGAGA ACAAGATCAC TGGATCGGCC 
CGCATTCGCT GGGCCTCCGA CCGGAAGAGC GCCATGGAAA CCGTGCTCCA GTACTGCAAA 
GGAAAAAATA TCAAGAGGCC GCCCCCCAAG TCTTACCTTA TCCATGCTGG TCTGGAGCCC 
TTGACATTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AGGACATTGC TGAAATCACA 
GAAATGGATA CCGAAGTTTC AAATCAGATC ACCCTTGTGG AAGATGTCTT AGCCAAGCTC 
TGTAAAACCA TCTATCCCCT GGCTGATCTG CTAGCCAGGC CGCTACCCGA GGGAGTTGAT 
CCTCTGAAGC TTGAGATTTA TCTCACCGAT GAAGACTTTG AGTTTGCACT AGACATGACA 
AGAGACGAAT ACAATGCTCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAGGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SVIL
Entrez Gene ID	477965
Full Name	supervillin
General protein information	Preferred Names supervillin Names supervillin
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Bos taurus (cattle)	SVIL	NP_776615.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007181	XP_565017.3
Danio rerio (zebrafish)	svilb	XP_003197996.2
Xenopus tropicalis (tropical clawed frog)	svil	NP_001090765.1
Pan troglodytes (chimpanzee)	SVIL	XP_001136112.1
Canis lupus familiaris (dog)	SVIL	XP_005617106.1
Mus musculus (house mouse)	Svil	NP_694793.1
Rattus norvegicus (Norway rat)	Svil	NP_001101886.2
Gallus gallus (chicken)	SVIL	XP_004939254.1
	SVIL	XP_001083894.2
Danio rerio (zebrafish)	svila	NP_001030338.2
Homo sapiens (human)	SVIL	NP_068506.2
Drosophila melanogaster (fruit fly)	CG33232	NP_995968.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SVIL cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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