TRHDE cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRHDE
Entrez Gene ID	467071
Full Name	thyrotropin releasing hormone degrading enzyme
General protein information	Preferred Names thyrotropin releasing hormone degrading enzyme Names thyrotropin-releasing hormone-degrading ectoenzyme
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016923855.1	XP_016779344.1	thyrotropin-releasing hormone-degrading ectoenzyme isoform X3
XM_016923853.1	XP_016779342.1	thyrotropin-releasing hormone-degrading ectoenzyme isoform X1
XM_016923854.1	XP_016779343.1	thyrotropin-releasing hormone-degrading ectoenzyme isoform X2
XM_016923853.2	XP_016779342.1	thyrotropin-releasing hormone-degrading ectoenzyme isoform X1
XM_016923854.2	XP_016779343.1	thyrotropin-releasing hormone-degrading ectoenzyme isoform X2
XM_016923855.2	XP_016779344.1	thyrotropin-releasing hormone-degrading ectoenzyme isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRHDE	NP_037513.1
Pan troglodytes (chimpanzee)	TRHDE	XP_522471.4
Canis lupus familiaris (dog)	TRHDE	XP_005625720.1
Xenopus tropicalis (tropical clawed frog)	trhde	XP_002939988.2
	TRHDE	XP_001109391.2
Bos taurus (cattle)	TRHDE	XP_002687216.3
Rattus norvegicus (Norway rat)	Trhde	NP_001102461.1
Mus musculus (house mouse)	Trhde	NP_666353.1
Gallus gallus (chicken)	TRHDE	XP_425442.3
Danio rerio (zebrafish)	LOC562409	XP_690889.2
Danio rerio (zebrafish)	si:ch211-149p5.1	XP_001345457.3

Gene Ontology

The following TRHDE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRHDE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc66227	XM_016923855.2 Latest version!	Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPc66225	XM_016923853.2 Latest version!	Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc66226	XM_016923854.2 Latest version!	Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OPc66225	XM_016923853.1	Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc66226	XM_016923854.1	Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc66227	XM_016923855.1	Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRHDE ( XM_016923855.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016779344.1 Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPc66227
Clone ID Related Accession (Same CDS sequence)	XM_016923855.1 , XM_016923855.2
Accession Version	XM_016923855.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	thyrotropin-releasing hormone-degrading ectoenzyme isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006479.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG ACGGCGAGCT GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAAAAG 
AAGAGGAAGA AGAAGAAGAA GAAGGAGGAG GAGGAGGAGG GGGCCGAGAA GAGCAGCTCA 
CCCTTTGCAG CCGCGATGGG GGAAGACGAC GCCGCGCTTC GGGCTGGCGG CAGGGGGCTC 
TCCGACCCGT GGGCAGACTC AGTGGGAGTG CGACCCCGCA CCACGGAGCG CCACATCGCC 
GTACACAAGC GGCTTGTGCT GGCCTTCGCC GTGTCCCTCG TGGCATTGCT CGCGGTCACA 
ATGCTCGCCG TGCTGCTCAG CCTGCGCTTC GACGAGTGCG GGGCGAGTGC CACGCCAGGC 
GCCGACGGTG GCCCCTCAGG CTTTCCGGAG CGCGGCGGCA ACGGGAGCCT CCCTGGATCG 
GCCCGGCGCA ACCACCACGC AGGCGGGGAC TCCTGGCAGC CCGAGGCGGG TGGGGTGGCC 
AGTCCGGGGA CCCCGTCGGC CCAGCCGCCG TCGGAGGAGG AGCGGGAGCC GTGGGAGCCG 
TGGACGCAGC TGCGCCTGTC GGGCCACCTG AAGCCGCTGC ACTACAATCT GATGCTCACC 
GCCTTCATGG AGAACTTCAC CTTCTCCGGG GAGGTCAACG TGGAGATCGC GTGCCGGAAC 
GCCACCCGCT ACGTAGTGCT GCACGCTTCC CGAGTGGCGG TGGAGAAAGT GCAGCTGGCC 
GAGGACCGGG CGTTCGGGGC TGTCCCTGTG GCCGGTTTTT TCCTCTACCC GCAAACCCAG 
GTCTTAGTGG TGGTGCTGAA TAGGACACTG GACGCGCAGA GGAATTACAA TCTGAAGATT 
ATCTATAACG CGCTCATCGA GAATGAGCTC CTGGGCTTCT TCCGCAGCTC CTATGTGCTC 
CACGGGGAGA GAAGATTCCT TGGTGTTACT CAGTTTTCGC CTACACATGC CAGAAAGGCA 
TTTCCTTGTT TTGATGAGCC AATCTACAAG GCTACTTTCA AAATCAGCAT CAAGCATCAA 
GCAACCTATT TATCTTTATC TAATATGCCA GTGGAAACTT CCGTGTTTGA GGAAGATGGA 
TGGGTTACGG ATCACTTTTC ACAGACCCCT CTCATGTCCA CATATTATTT AGCCTGGGCA 
ATTTGCAACT TCACATACAG AGAAACTACC ACCAAGAGTG GGGTTGTAGT ACGATTATAT 
GCAAGACCTG ATGCTATCAG AAGAGGATCC GGGGACTATG CTCTCCATAT AACAAAGAGA 
TTAATAGAAT TTTATGAAGA CTACTTTAAA GTGCCCTATT CCTTGCCAAA ACTAGATCTT 
TTAGCTGTGC CTAAGCATCC GTATGCTGCT ATGGAGAACT GGGGACTAAG TATTTTTGTG 
GAACAAAGAA TACTGCTGGA TCCCAGTGTT TCATCTATTT CTTATTTGCT GGATGTCACC 
ATGGTCATTG TTCATGAGAT ATGTCACCAG TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG 
TGGGAAGACG TGTGGCTGAA GGAAGGGTTT GCTCACTACT TTGAATTTGT TGGTACAGAC 
TACCTCTATC CTGGCTGGAA CATGGAAAAG CAGAGGTTTC TGACCGATGT TCTGCATGAA 
GTGATGCTGC TGGACGGTTT GGCCAGTTCC CATCCAGTAT CACAGGAAGT GCTGCAGGCA 
ACAGATATTG ACAGGGTGTT TGACTGGATC GCATATAAAA AGGGTGCTGC TTTAATAAGA 
ATGCTGGCTA ATTTTATGGG CCATTCAGTT TTCCAGAGGG GTTTGCAAGA TTATTTAACC 
ATTCATAAGT ATGGCAATGC AGCCAGAAAT GATCTCTGGA ATACATTATC GGAGGCATTA 
AAAAGAAATG GGAAATATGT AAATATACAA GAAGTAATGG ATCAGTGGAC ACTCCAGATG 
GGTTATCCTG TTATCACCAT CTTGGGAAAC ACAACAGCAG AAAATAGAAT AATAATTACC 
CAACAGCATT TTATCTATGA TATCAGTGCT AAAACTAAAG CACTTAAACT TCAGAATAAC 
AGTTACCTGT GGCAGATTCC ATTAACTATT GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA 
GAAGCAATTA TTTGGGTGTC TAACAAATCA GAGCACCACA GAATAACTTA TTTGGACAAA 
GGAAGCTGGC TGCTGGGGAA CATCAATCAA ACTGGCTATT TTAGAGTCAA CTATGACCTA 
AGGAACTGGA GATTATTAAT TGATCAATTA ATCCGGAATC ATGAGGTTCT TTCTGTCAGT 
AACCGAGCGG GCTTGATCGA TGATGCCTTC AGCCTAGCCA GGGCTGGCTA TTTGCCTCAG 
AATATTCCTC TGGAGATTAT CAGATACCTG TCTGAGGAGA AGGATTTTCT TCCTTGGCAT 
GCTGCCAGCC GAGCTCTTTA TCCTCTAGAT AAATTACTGG ACCGCATGGA AAACTACAAC 
ATTTTCAATG AATATATCTT AAAGCAAGTT GCAACAACGT ATATCAAGCT TGGGTGGCCG 
AAAAATAATT TTAATGGATC TCTTGTTCAA GCATCCTACC AACATGAAGA ACTACGTAGA 
GAAGTTATAA TGCTGGCCTG CAGTTTTGGC AACAAGCACT GTCACCAACA GGCATCAACA 
CTTATTTCAG ATTGGATTTC CAGCAACAGG AACAGTACTG TTAAGCTTTT AATTAATAGA 
AAACCACCAT GGGTTGCTTC TTTAGAATAC CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016779344.1
CDS	221..2956
Translation	MALDGELGEQEEEKKKKKKKKRKKKKKKEEEEEGAEKSSSPFAAAMGEDDAALRAGGRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSLVALLAVTMLAVLLSLRFDECGASATPGADGGPSGFPERGGNGSLPGSARRNHHAGGDSWQPEAGGVASPGTPSAQPPSEEEREPWEPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQLAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRNYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKALKLQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNIFNEYILKQVATTYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNSTVKLLINRKPPWVASLEYH

Target ORF information:

RefSeq Version	XM_016923855.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016923855.1

ORF Insert Sequence:

ATGGCCCTGG ACGGCGAGCT GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAAAAG 
AAGAGGAAGA AGAAGAAGAA GAAGGAGGAG GAGGAGGAGG GGGCCGAGAA GAGCAGCTCA 
CCCTTTGCAG CCGCGATGGG GGAAGACGAC GCCGCGCTTC GGGCTGGCGG CAGGGGGCTC 
TCCGACCCGT GGGCAGACTC AGTGGGAGTG CGACCCCGCA CCACGGAGCG CCACATCGCC 
GTACACAAGC GGCTTGTGCT GGCCTTCGCC GTGTCCCTCG TGGCATTGCT CGCGGTCACA 
ATGCTCGCCG TGCTGCTCAG CCTGCGCTTC GACGAGTGCG GGGCGAGTGC CACGCCAGGC 
GCCGACGGTG GCCCCTCAGG CTTTCCGGAG CGCGGCGGCA ACGGGAGCCT CCCTGGATCG 
GCCCGGCGCA ACCACCACGC AGGCGGGGAC TCCTGGCAGC CCGAGGCGGG TGGGGTGGCC 
AGTCCGGGGA CCCCGTCGGC CCAGCCGCCG TCGGAGGAGG AGCGGGAGCC GTGGGAGCCG 
TGGACGCAGC TGCGCCTGTC GGGCCACCTG AAGCCGCTGC ACTACAATCT GATGCTCACC 
GCCTTCATGG AGAACTTCAC CTTCTCCGGG GAGGTCAACG TGGAGATCGC GTGCCGGAAC 
GCCACCCGCT ACGTAGTGCT GCACGCTTCC CGAGTGGCGG TGGAGAAAGT GCAGCTGGCC 
GAGGACCGGG CGTTCGGGGC TGTCCCTGTG GCCGGTTTTT TCCTCTACCC GCAAACCCAG 
GTCTTAGTGG TGGTGCTGAA TAGGACACTG GACGCGCAGA GGAATTACAA TCTGAAGATT 
ATCTATAACG CGCTCATCGA GAATGAGCTC CTGGGCTTCT TCCGCAGCTC CTATGTGCTC 
CACGGGGAGA GAAGATTCCT TGGTGTTACT CAGTTTTCGC CTACACATGC CAGAAAGGCA 
TTTCCTTGTT TTGATGAGCC AATCTACAAG GCTACTTTCA AAATCAGCAT CAAGCATCAA 
GCAACCTATT TATCTTTATC TAATATGCCA GTGGAAACTT CCGTGTTTGA GGAAGATGGA 
TGGGTTACGG ATCACTTTTC ACAGACCCCT CTCATGTCCA CATATTATTT AGCCTGGGCA 
ATTTGCAACT TCACATACAG AGAAACTACC ACCAAGAGTG GGGTTGTAGT ACGATTATAT 
GCAAGACCTG ATGCTATCAG AAGAGGATCC GGGGACTATG CTCTCCATAT AACAAAGAGA 
TTAATAGAAT TTTATGAAGA CTACTTTAAA GTGCCCTATT CCTTGCCAAA ACTAGATCTT 
TTAGCTGTGC CTAAGCATCC GTATGCTGCT ATGGAGAACT GGGGACTAAG TATTTTTGTG 
GAACAAAGAA TACTGCTGGA TCCCAGTGTT TCATCTATTT CTTATTTGCT GGATGTCACC 
ATGGTCATTG TTCATGAGAT ATGTCACCAG TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG 
TGGGAAGACG TGTGGCTGAA GGAAGGGTTT GCTCACTACT TTGAATTTGT TGGTACAGAC 
TACCTCTATC CTGGCTGGAA CATGGAAAAG CAGAGGTTTC TGACCGATGT TCTGCATGAA 
GTGATGCTGC TGGACGGTTT GGCCAGTTCC CATCCAGTAT CACAGGAAGT GCTGCAGGCA 
ACAGATATTG ACAGGGTGTT TGACTGGATC GCATATAAAA AGGGTGCTGC TTTAATAAGA 
ATGCTGGCTA ATTTTATGGG CCATTCAGTT TTCCAGAGGG GTTTGCAAGA TTATTTAACC 
ATTCATAAGT ATGGCAATGC AGCCAGAAAT GATCTCTGGA ATACATTATC GGAGGCATTA 
AAAAGAAATG GGAAATATGT AAATATACAA GAAGTAATGG ATCAGTGGAC ACTCCAGATG 
GGTTATCCTG TTATCACCAT CTTGGGAAAC ACAACAGCAG AAAATAGAAT AATAATTACC 
CAACAGCATT TTATCTATGA TATCAGTGCT AAAACTAAAG CACTTAAACT TCAGAATAAC 
AGTTACCTGT GGCAGATTCC ATTAACTATT GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA 
GAAGCAATTA TTTGGGTGTC TAACAAATCA GAGCACCACA GAATAACTTA TTTGGACAAA 
GGAAGCTGGC TGCTGGGGAA CATCAATCAA ACTGGCTATT TTAGAGTCAA CTATGACCTA 
AGGAACTGGA GATTATTAAT TGATCAATTA ATCCGGAATC ATGAGGTTCT TTCTGTCAGT 
AACCGAGCGG GCTTGATCGA TGATGCCTTC AGCCTAGCCA GGGCTGGCTA TTTGCCTCAG 
AATATTCCTC TGGAGATTAT CAGATACCTG TCTGAGGAGA AGGATTTTCT TCCTTGGCAT 
GCTGCCAGCC GAGCTCTTTA TCCTCTAGAT AAATTACTGG ACCGCATGGA AAACTACAAC 
ATTTTCAATG AATATATCTT AAAGCAAGTT GCAACAACGT ATATCAAGCT TGGGTGGCCG 
AAAAATAATT TTAATGGATC TCTTGTTCAA GCATCCTACC AACATGAAGA ACTACGTAGA 
GAAGTTATAA TGCTGGCCTG CAGTTTTGGC AACAAGCACT GTCACCAACA GGCATCAACA 
CTTATTTCAG ATTGGATTTC CAGCAACAGG AACAGTACTG TTAAGCTTTT AATTAATAGA 
AAACCACCAT GGGTTGCTTC TTTAGAATAC CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRHDE ( XM_016923855.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016779344.1 Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPc66227
Clone ID Related Accession (Same CDS sequence)	XM_016923855.1 , XM_016923855.2
Accession Version	XM_016923855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	thyrotropin-releasing hormone-degrading ectoenzyme isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016923855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG ACGGCGAGCT GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAAAAG 
AAGAGGAAGA AGAAGAAGAA GAAGGAGGAG GAGGAGGAGG GGGCCGAGAA GAGCAGCTCA 
CCCTTTGCAG CCGCGATGGG GGAAGACGAC GCCGCGCTTC GGGCTGGCGG CAGGGGGCTC 
TCCGACCCGT GGGCAGACTC AGTGGGAGTG CGACCCCGCA CCACGGAGCG CCACATCGCC 
GTACACAAGC GGCTTGTGCT GGCCTTCGCC GTGTCCCTCG TGGCATTGCT CGCGGTCACA 
ATGCTCGCCG TGCTGCTCAG CCTGCGCTTC GACGAGTGCG GGGCGAGTGC CACGCCAGGC 
GCCGACGGTG GCCCCTCAGG CTTTCCGGAG CGCGGCGGCA ACGGGAGCCT CCCTGGATCG 
GCCCGGCGCA ACCACCACGC AGGCGGGGAC TCCTGGCAGC CCGAGGCGGG TGGGGTGGCC 
AGTCCGGGGA CCCCGTCGGC CCAGCCGCCG TCGGAGGAGG AGCGGGAGCC GTGGGAGCCG 
TGGACGCAGC TGCGCCTGTC GGGCCACCTG AAGCCGCTGC ACTACAATCT GATGCTCACC 
GCCTTCATGG AGAACTTCAC CTTCTCCGGG GAGGTCAACG TGGAGATCGC GTGCCGGAAC 
GCCACCCGCT ACGTAGTGCT GCACGCTTCC CGAGTGGCGG TGGAGAAAGT GCAGCTGGCC 
GAGGACCGGG CGTTCGGGGC TGTCCCTGTG GCCGGTTTTT TCCTCTACCC GCAAACCCAG 
GTCTTAGTGG TGGTGCTGAA TAGGACACTG GACGCGCAGA GGAATTACAA TCTGAAGATT 
ATCTATAACG CGCTCATCGA GAATGAGCTC CTGGGCTTCT TCCGCAGCTC CTATGTGCTC 
CACGGGGAGA GAAGATTCCT TGGTGTTACT CAGTTTTCGC CTACACATGC CAGAAAGGCA 
TTTCCTTGTT TTGATGAGCC AATCTACAAG GCTACTTTCA AAATCAGCAT CAAGCATCAA 
GCAACCTATT TATCTTTATC TAATATGCCA GTGGAAACTT CCGTGTTTGA GGAAGATGGA 
TGGGTTACGG ATCACTTTTC ACAGACCCCT CTCATGTCCA CATATTATTT AGCCTGGGCA 
ATTTGCAACT TCACATACAG AGAAACTACC ACCAAGAGTG GGGTTGTAGT ACGATTATAT 
GCAAGACCTG ATGCTATCAG AAGAGGATCC GGGGACTATG CTCTCCATAT AACAAAGAGA 
TTAATAGAAT TTTATGAAGA CTACTTTAAA GTGCCCTATT CCTTGCCAAA ACTAGATCTT 
TTAGCTGTGC CTAAGCATCC GTATGCTGCT ATGGAGAACT GGGGACTAAG TATTTTTGTG 
GAACAAAGAA TACTGCTGGA TCCCAGTGTT TCATCTATTT CTTATTTGCT GGATGTCACC 
ATGGTCATTG TTCATGAGAT ATGTCACCAG TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG 
TGGGAAGACG TGTGGCTGAA GGAAGGGTTT GCTCACTACT TTGAATTTGT TGGTACAGAC 
TACCTCTATC CTGGCTGGAA CATGGAAAAG CAGAGGTTTC TGACCGATGT TCTGCATGAA 
GTGATGCTGC TGGACGGTTT GGCCAGTTCC CATCCAGTAT CACAGGAAGT GCTGCAGGCA 
ACAGATATTG ACAGGGTGTT TGACTGGATC GCATATAAAA AGGGTGCTGC TTTAATAAGA 
ATGCTGGCTA ATTTTATGGG CCATTCAGTT TTCCAGAGGG GTTTGCAAGA TTATTTAACC 
ATTCATAAGT ATGGCAATGC AGCCAGAAAT GATCTCTGGA ATACATTATC GGAGGCATTA 
AAAAGAAATG GGAAATATGT AAATATACAA GAAGTAATGG ATCAGTGGAC ACTCCAGATG 
GGTTATCCTG TTATCACCAT CTTGGGAAAC ACAACAGCAG AAAATAGAAT AATAATTACC 
CAACAGCATT TTATCTATGA TATCAGTGCT AAAACTAAAG CACTTAAACT TCAGAATAAC 
AGTTACCTGT GGCAGATTCC ATTAACTATT GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA 
GAAGCAATTA TTTGGGTGTC TAACAAATCA GAGCACCACA GAATAACTTA TTTGGACAAA 
GGAAGCTGGC TGCTGGGGAA CATCAATCAA ACTGGCTATT TTAGAGTCAA CTATGACCTA 
AGGAACTGGA GATTATTAAT TGATCAATTA ATCCGGAATC ATGAGGTTCT TTCTGTCAGT 
AACCGAGCGG GCTTGATCGA TGATGCCTTC AGCCTAGCCA GGGCTGGCTA TTTGCCTCAG 
AATATTCCTC TGGAGATTAT CAGATACCTG TCTGAGGAGA AGGATTTTCT TCCTTGGCAT 
GCTGCCAGCC GAGCTCTTTA TCCTCTAGAT AAATTACTGG ACCGCATGGA AAACTACAAC 
ATTTTCAATG AATATATCTT AAAGCAAGTT GCAACAACGT ATATCAAGCT TGGGTGGCCG 
AAAAATAATT TTAATGGATC TCTTGTTCAA GCATCCTACC AACATGAAGA ACTACGTAGA 
GAAGTTATAA TGCTGGCCTG CAGTTTTGGC AACAAGCACT GTCACCAACA GGCATCAACA 
CTTATTTCAG ATTGGATTTC CAGCAACAGG AACAGTACTG TTAAGCTTTT AATTAATAGA 
AAACCACCAT GGGTTGCTTC TTTAGAATAC CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016779344.1
CDS	124..2859
Translation	MALDGELGEQEEEKKKKKKKKRKKKKKKEEEEEGAEKSSSPFAAAMGEDDAALRAGGRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSLVALLAVTMLAVLLSLRFDECGASATPGADGGPSGFPERGGNGSLPGSARRNHHAGGDSWQPEAGGVASPGTPSAQPPSEEEREPWEPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQLAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRNYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKALKLQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNIFNEYILKQVATTYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNSTVKLLINRKPPWVASLEYH

Target ORF information:

RefSeq Version	XM_016923855.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016923855.2

ORF Insert Sequence:

ATGGCCCTGG ACGGCGAGCT GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAAAAG 
AAGAGGAAGA AGAAGAAGAA GAAGGAGGAG GAGGAGGAGG GGGCCGAGAA GAGCAGCTCA 
CCCTTTGCAG CCGCGATGGG GGAAGACGAC GCCGCGCTTC GGGCTGGCGG CAGGGGGCTC 
TCCGACCCGT GGGCAGACTC AGTGGGAGTG CGACCCCGCA CCACGGAGCG CCACATCGCC 
GTACACAAGC GGCTTGTGCT GGCCTTCGCC GTGTCCCTCG TGGCATTGCT CGCGGTCACA 
ATGCTCGCCG TGCTGCTCAG CCTGCGCTTC GACGAGTGCG GGGCGAGTGC CACGCCAGGC 
GCCGACGGTG GCCCCTCAGG CTTTCCGGAG CGCGGCGGCA ACGGGAGCCT CCCTGGATCG 
GCCCGGCGCA ACCACCACGC AGGCGGGGAC TCCTGGCAGC CCGAGGCGGG TGGGGTGGCC 
AGTCCGGGGA CCCCGTCGGC CCAGCCGCCG TCGGAGGAGG AGCGGGAGCC GTGGGAGCCG 
TGGACGCAGC TGCGCCTGTC GGGCCACCTG AAGCCGCTGC ACTACAATCT GATGCTCACC 
GCCTTCATGG AGAACTTCAC CTTCTCCGGG GAGGTCAACG TGGAGATCGC GTGCCGGAAC 
GCCACCCGCT ACGTAGTGCT GCACGCTTCC CGAGTGGCGG TGGAGAAAGT GCAGCTGGCC 
GAGGACCGGG CGTTCGGGGC TGTCCCTGTG GCCGGTTTTT TCCTCTACCC GCAAACCCAG 
GTCTTAGTGG TGGTGCTGAA TAGGACACTG GACGCGCAGA GGAATTACAA TCTGAAGATT 
ATCTATAACG CGCTCATCGA GAATGAGCTC CTGGGCTTCT TCCGCAGCTC CTATGTGCTC 
CACGGGGAGA GAAGATTCCT TGGTGTTACT CAGTTTTCGC CTACACATGC CAGAAAGGCA 
TTTCCTTGTT TTGATGAGCC AATCTACAAG GCTACTTTCA AAATCAGCAT CAAGCATCAA 
GCAACCTATT TATCTTTATC TAATATGCCA GTGGAAACTT CCGTGTTTGA GGAAGATGGA 
TGGGTTACGG ATCACTTTTC ACAGACCCCT CTCATGTCCA CATATTATTT AGCCTGGGCA 
ATTTGCAACT TCACATACAG AGAAACTACC ACCAAGAGTG GGGTTGTAGT ACGATTATAT 
GCAAGACCTG ATGCTATCAG AAGAGGATCC GGGGACTATG CTCTCCATAT AACAAAGAGA 
TTAATAGAAT TTTATGAAGA CTACTTTAAA GTGCCCTATT CCTTGCCAAA ACTAGATCTT 
TTAGCTGTGC CTAAGCATCC GTATGCTGCT ATGGAGAACT GGGGACTAAG TATTTTTGTG 
GAACAAAGAA TACTGCTGGA TCCCAGTGTT TCATCTATTT CTTATTTGCT GGATGTCACC 
ATGGTCATTG TTCATGAGAT ATGTCACCAG TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG 
TGGGAAGACG TGTGGCTGAA GGAAGGGTTT GCTCACTACT TTGAATTTGT TGGTACAGAC 
TACCTCTATC CTGGCTGGAA CATGGAAAAG CAGAGGTTTC TGACCGATGT TCTGCATGAA 
GTGATGCTGC TGGACGGTTT GGCCAGTTCC CATCCAGTAT CACAGGAAGT GCTGCAGGCA 
ACAGATATTG ACAGGGTGTT TGACTGGATC GCATATAAAA AGGGTGCTGC TTTAATAAGA 
ATGCTGGCTA ATTTTATGGG CCATTCAGTT TTCCAGAGGG GTTTGCAAGA TTATTTAACC 
ATTCATAAGT ATGGCAATGC AGCCAGAAAT GATCTCTGGA ATACATTATC GGAGGCATTA 
AAAAGAAATG GGAAATATGT AAATATACAA GAAGTAATGG ATCAGTGGAC ACTCCAGATG 
GGTTATCCTG TTATCACCAT CTTGGGAAAC ACAACAGCAG AAAATAGAAT AATAATTACC 
CAACAGCATT TTATCTATGA TATCAGTGCT AAAACTAAAG CACTTAAACT TCAGAATAAC 
AGTTACCTGT GGCAGATTCC ATTAACTATT GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA 
GAAGCAATTA TTTGGGTGTC TAACAAATCA GAGCACCACA GAATAACTTA TTTGGACAAA 
GGAAGCTGGC TGCTGGGGAA CATCAATCAA ACTGGCTATT TTAGAGTCAA CTATGACCTA 
AGGAACTGGA GATTATTAAT TGATCAATTA ATCCGGAATC ATGAGGTTCT TTCTGTCAGT 
AACCGAGCGG GCTTGATCGA TGATGCCTTC AGCCTAGCCA GGGCTGGCTA TTTGCCTCAG 
AATATTCCTC TGGAGATTAT CAGATACCTG TCTGAGGAGA AGGATTTTCT TCCTTGGCAT 
GCTGCCAGCC GAGCTCTTTA TCCTCTAGAT AAATTACTGG ACCGCATGGA AAACTACAAC 
ATTTTCAATG AATATATCTT AAAGCAAGTT GCAACAACGT ATATCAAGCT TGGGTGGCCG 
AAAAATAATT TTAATGGATC TCTTGTTCAA GCATCCTACC AACATGAAGA ACTACGTAGA 
GAAGTTATAA TGCTGGCCTG CAGTTTTGGC AACAAGCACT GTCACCAACA GGCATCAACA 
CTTATTTCAG ATTGGATTTC CAGCAACAGG AACAGTACTG TTAAGCTTTT AATTAATAGA 
AAACCACCAT GGGTTGCTTC TTTAGAATAC CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRHDE ( XM_016923853.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016779342.1 Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc66225
Clone ID Related Accession (Same CDS sequence)	XM_016923853.1 , XM_016923853.2
Accession Version	XM_016923853.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	thyrotropin-releasing hormone-degrading ectoenzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006479.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG ACGGCGAGCT GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAAAAG 
AAGAGGAAGA AGAAGAAGAA GAAGGAGGAG GAGGAGGAGG GGGCCGAGAA GAGCAGCTCA 
CCCTTTGCAG CCGCGATGGG GGAAGACGAC GCCGCGCTTC GGGCTGGCGG CAGGGGGCTC 
TCCGACCCGT GGGCAGACTC AGTGGGAGTG CGACCCCGCA CCACGGAGCG CCACATCGCC 
GTACACAAGC GGCTTGTGCT GGCCTTCGCC GTGTCCCTCG TGGCATTGCT CGCGGTCACA 
ATGCTCGCCG TGCTGCTCAG CCTGCGCTTC GACGAGTGCG GGGCGAGTGC CACGCCAGGC 
GCCGACGGTG GCCCCTCAGG CTTTCCGGAG CGCGGCGGCA ACGGGAGCCT CCCTGGATCG 
GCCCGGCGCA ACCACCACGC AGGCGGGGAC TCCTGGCAGC CCGAGGCGGG TGGGGTGGCC 
AGTCCGGGGA CCCCGTCGGC CCAGCCGCCG TCGGAGGAGG AGCGGGAGCC GTGGGAGCCG 
TGGACGCAGC TGCGCCTGTC GGGCCACCTG AAGCCGCTGC ACTACAATCT GATGCTCACC 
GCCTTCATGG AGAACTTCAC CTTCTCCGGG GAGGTCAACG TGGAGATCGC GTGCCGGAAC 
GCCACCCGCT ACGTAGTGCT GCACGCTTCC CGAGTGGCGG TGGAGAAAGT GCAGCTGGCC 
GAGGACCGGG CGTTCGGGGC TGTCCCTGTG GCCGGTTTTT TCCTCTACCC GCAAACCCAG 
GTCTTAGTGG TGGTGCTGAA TAGGACACTG GACGCGCAGA GGAATTACAA TCTGAAGATT 
ATCTATAACG CGCTCATCGA GAATGAGCTC CTGGGCTTCT TCCGCAGCTC CTATGTGCTC 
CACGGGGAGA GAAGATTCCT TGGTGTTACT CAGTTTTCGC CTACACATGC CAGAAAGGCA 
TTTCCTTGTT TTGATGAGCC AATCTACAAG GCTACTTTCA AAATCAGCAT CAAGCATCAA 
GCAACCTATT TATCTTTATC TAATATGCCA GTGGAAACTT CCGTGTTTGA GGAAGATGGA 
TGGGTTACGG ATCACTTTTC ACAGACCCCT CTCATGTCCA CATATTATTT AGCCTGGGCA 
ATTTGCAACT TCACATACAG AGAAACTACC ACCAAGAGTG GGGTTGTAGT ACGATTATAT 
GCAAGACCTG ATGCTATCAG AAGAGGATCC GGGGACTATG CTCTCCATAT AACAAAGAGA 
TTAATAGAAT TTTATGAAGA CTACTTTAAA GTGCCCTATT CCTTGCCAAA ACTAGATCTT 
TTAGCTGTGC CTAAGCATCC GTATGCTGCT ATGGAGAACT GGGGACTAAG TATTTTTGTG 
GAACAAAGAA TACTGCTGGA TCCCAGTGTT TCATCTATTT CTTATTTGCT GGATGTCACC 
ATGGTCATTG TTCATGAGAT ATGTCACCAG TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG 
TGGGAAGACG TGTGGCTGAA GGAAGGGTTT GCTCACTACT TTGAATTTGT TGGTACAGAC 
TACCTCTATC CTGGCTGGAA CATGGAAAAG CAGAGGTTTC TGACCGATGT TCTGCATGAA 
GTGATGCTGC TGGACGGTTT GGCCAGTTCC CATCCAGTAT CACAGGAAGT GCTGCAGGCA 
ACAGATATTG ACAGGGTGTT TGACTGGATC GCATATAAAA AGGGTGCTGC TTTAATAAGA 
ATGCTGGCTA ATTTTATGGG CCATTCAGTT TTCCAGAGGG GTTTGCAAGA TTATTTAACC 
ATTCATAAGT ATGGCAATGC AGCCAGAAAT GATCTCTGGA ATACATTATC GGAGGCATTA 
AAAAGAAATG GGAAATATGT AAATATACAA GAAGTAATGG ATCAGTGGAC ACTCCAGATG 
GGTTATCCTG TTATCACCAT CTTGGGAAAC ACAACAGCAG AAAATAGAAT AATAATTACC 
CAACAGCATT TTATCTATGA TATCAGTGCT AAAACTAAAG CACTTAAACT TCAGAATAAC 
AGTTACCTGT GGCAGATTCC ATTAACTATT GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA 
GAAGCAATTA TTTGGGTGTC TAACAAATCA GAGCACCACA GAATAACTTA TTTGGACAAA 
GGAAGCTGGC TGCTGGGGAA CATCAATCAA ACTGGCTATT TTAGAGTCAA CTATGACCTA 
AGGAACTGGA GATTATTAAT TGATCAATTA ATCCGGAATC ATGAGGTTCT TTCTGTCAGT 
AACCGAGCGG GCTTGATCGA TGATGCCTTC AGCCTAGCCA GGGCTGGCTA TTTGCCTCAG 
AATATTCCTC TGGAGATTAT CAGATACCTG TCTGAGGAGA AGGATTTTCT TCCTTGGCAT 
GCTGCCAGCC GAGCTCTTTA TCCTCTAGAT AAATTACTGG ACCGCATGGA AAACTACAAC 
ATTTTCAATG AATATATCTT AAAGCAAGTT GCAACAACGT ATATCAAGCT TGGGTGGCCG 
AAAAATAATT TTAATGGATC TCTTGTTCAA GCATCCTACC AACATGAAGA ACTACGTAGA 
GAAGTTATAA TGCTGGCCTG CAGTTTTGGC AACAAGCACT GTCACCAACA GGCATCAACA 
CTTATTTCAG ATTGGATTTC CAGCAACAGG AACAGAATAC CACTAAATGT TAGAGACATC 
GTATACTGTA CAGGAGTGTC ACTGCTGGAT GAGGATGTCT GGGAATTCAT ATGGATGAAA 
TTCCATTCCA CCACAGCAGT TTCTGAGAAG AAAATATTAT TGGAAGCCTT AACTTGCAGT 
GATGACAGGA ATTTATTAAA CAGGCTTCTA AATCTGTCAC TGAATTCTGA GGTGGTGCTG 
GATCAAGATG CAATTGATGT CATAATCCAT GTAGCTCGAA ATCCACATGG TCGAGACCTT 
GCCTGGAAGT TTTTCAGGGA TAAATGGAAG ATATTAAATA CCAGGTATGG AGAAGCATTG 
TTTATGAATT CCAAACTCAT CAGTGGTGTC ACAGAATTTC TTAATACTGA AGGTGAACTC 
AAAGAGCTCA AGAACTTCAT GAAAAACTAT GATGGGGTAG CTGCTGCTTC TTTCTCACGA 
GCTGTGGAAA CTGTTGAAGC CAATGTGCGC TGGAAAATGC TTTACCAAGA CGAGCTTTTC 
CAATGGTTAG GAAAAGCTCT AAGACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016779342.1
CDS	221..3430
Translation	MALDGELGEQEEEKKKKKKKKRKKKKKKEEEEEGAEKSSSPFAAAMGEDDAALRAGGRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSLVALLAVTMLAVLLSLRFDECGASATPGADGGPSGFPERGGNGSLPGSARRNHHAGGDSWQPEAGGVASPGTPSAQPPSEEEREPWEPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQLAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRNYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKALKLQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNIFNEYILKQVATTYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLNRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTRYGEALFMNSKLISGVTEFLNTEGELKELKNFMKNYDGVAAASFSRAVETVEANVRWKMLYQDELFQWLGKALRH

Target ORF information:

RefSeq Version	XM_016923853.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016923853.1

ORF Insert Sequence:

ATGGCCCTGG ACGGCGAGCT GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAAAAG 
AAGAGGAAGA AGAAGAAGAA GAAGGAGGAG GAGGAGGAGG GGGCCGAGAA GAGCAGCTCA 
CCCTTTGCAG CCGCGATGGG GGAAGACGAC GCCGCGCTTC GGGCTGGCGG CAGGGGGCTC 
TCCGACCCGT GGGCAGACTC AGTGGGAGTG CGACCCCGCA CCACGGAGCG CCACATCGCC 
GTACACAAGC GGCTTGTGCT GGCCTTCGCC GTGTCCCTCG TGGCATTGCT CGCGGTCACA 
ATGCTCGCCG TGCTGCTCAG CCTGCGCTTC GACGAGTGCG GGGCGAGTGC CACGCCAGGC 
GCCGACGGTG GCCCCTCAGG CTTTCCGGAG CGCGGCGGCA ACGGGAGCCT CCCTGGATCG 
GCCCGGCGCA ACCACCACGC AGGCGGGGAC TCCTGGCAGC CCGAGGCGGG TGGGGTGGCC 
AGTCCGGGGA CCCCGTCGGC CCAGCCGCCG TCGGAGGAGG AGCGGGAGCC GTGGGAGCCG 
TGGACGCAGC TGCGCCTGTC GGGCCACCTG AAGCCGCTGC ACTACAATCT GATGCTCACC 
GCCTTCATGG AGAACTTCAC CTTCTCCGGG GAGGTCAACG TGGAGATCGC GTGCCGGAAC 
GCCACCCGCT ACGTAGTGCT GCACGCTTCC CGAGTGGCGG TGGAGAAAGT GCAGCTGGCC 
GAGGACCGGG CGTTCGGGGC TGTCCCTGTG GCCGGTTTTT TCCTCTACCC GCAAACCCAG 
GTCTTAGTGG TGGTGCTGAA TAGGACACTG GACGCGCAGA GGAATTACAA TCTGAAGATT 
ATCTATAACG CGCTCATCGA GAATGAGCTC CTGGGCTTCT TCCGCAGCTC CTATGTGCTC 
CACGGGGAGA GAAGATTCCT TGGTGTTACT CAGTTTTCGC CTACACATGC CAGAAAGGCA 
TTTCCTTGTT TTGATGAGCC AATCTACAAG GCTACTTTCA AAATCAGCAT CAAGCATCAA 
GCAACCTATT TATCTTTATC TAATATGCCA GTGGAAACTT CCGTGTTTGA GGAAGATGGA 
TGGGTTACGG ATCACTTTTC ACAGACCCCT CTCATGTCCA CATATTATTT AGCCTGGGCA 
ATTTGCAACT TCACATACAG AGAAACTACC ACCAAGAGTG GGGTTGTAGT ACGATTATAT 
GCAAGACCTG ATGCTATCAG AAGAGGATCC GGGGACTATG CTCTCCATAT AACAAAGAGA 
TTAATAGAAT TTTATGAAGA CTACTTTAAA GTGCCCTATT CCTTGCCAAA ACTAGATCTT 
TTAGCTGTGC CTAAGCATCC GTATGCTGCT ATGGAGAACT GGGGACTAAG TATTTTTGTG 
GAACAAAGAA TACTGCTGGA TCCCAGTGTT TCATCTATTT CTTATTTGCT GGATGTCACC 
ATGGTCATTG TTCATGAGAT ATGTCACCAG TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG 
TGGGAAGACG TGTGGCTGAA GGAAGGGTTT GCTCACTACT TTGAATTTGT TGGTACAGAC 
TACCTCTATC CTGGCTGGAA CATGGAAAAG CAGAGGTTTC TGACCGATGT TCTGCATGAA 
GTGATGCTGC TGGACGGTTT GGCCAGTTCC CATCCAGTAT CACAGGAAGT GCTGCAGGCA 
ACAGATATTG ACAGGGTGTT TGACTGGATC GCATATAAAA AGGGTGCTGC TTTAATAAGA 
ATGCTGGCTA ATTTTATGGG CCATTCAGTT TTCCAGAGGG GTTTGCAAGA TTATTTAACC 
ATTCATAAGT ATGGCAATGC AGCCAGAAAT GATCTCTGGA ATACATTATC GGAGGCATTA 
AAAAGAAATG GGAAATATGT AAATATACAA GAAGTAATGG ATCAGTGGAC ACTCCAGATG 
GGTTATCCTG TTATCACCAT CTTGGGAAAC ACAACAGCAG AAAATAGAAT AATAATTACC 
CAACAGCATT TTATCTATGA TATCAGTGCT AAAACTAAAG CACTTAAACT TCAGAATAAC 
AGTTACCTGT GGCAGATTCC ATTAACTATT GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA 
GAAGCAATTA TTTGGGTGTC TAACAAATCA GAGCACCACA GAATAACTTA TTTGGACAAA 
GGAAGCTGGC TGCTGGGGAA CATCAATCAA ACTGGCTATT TTAGAGTCAA CTATGACCTA 
AGGAACTGGA GATTATTAAT TGATCAATTA ATCCGGAATC ATGAGGTTCT TTCTGTCAGT 
AACCGAGCGG GCTTGATCGA TGATGCCTTC AGCCTAGCCA GGGCTGGCTA TTTGCCTCAG 
AATATTCCTC TGGAGATTAT CAGATACCTG TCTGAGGAGA AGGATTTTCT TCCTTGGCAT 
GCTGCCAGCC GAGCTCTTTA TCCTCTAGAT AAATTACTGG ACCGCATGGA AAACTACAAC 
ATTTTCAATG AATATATCTT AAAGCAAGTT GCAACAACGT ATATCAAGCT TGGGTGGCCG 
AAAAATAATT TTAATGGATC TCTTGTTCAA GCATCCTACC AACATGAAGA ACTACGTAGA 
GAAGTTATAA TGCTGGCCTG CAGTTTTGGC AACAAGCACT GTCACCAACA GGCATCAACA 
CTTATTTCAG ATTGGATTTC CAGCAACAGG AACAGAATAC CACTAAATGT TAGAGACATC 
GTATACTGTA CAGGAGTGTC ACTGCTGGAT GAGGATGTCT GGGAATTCAT ATGGATGAAA 
TTCCATTCCA CCACAGCAGT TTCTGAGAAG AAAATATTAT TGGAAGCCTT AACTTGCAGT 
GATGACAGGA ATTTATTAAA CAGGCTTCTA AATCTGTCAC TGAATTCTGA GGTGGTGCTG 
GATCAAGATG CAATTGATGT CATAATCCAT GTAGCTCGAA ATCCACATGG TCGAGACCTT 
GCCTGGAAGT TTTTCAGGGA TAAATGGAAG ATATTAAATA CCAGGTATGG AGAAGCATTG 
TTTATGAATT CCAAACTCAT CAGTGGTGTC ACAGAATTTC TTAATACTGA AGGTGAACTC 
AAAGAGCTCA AGAACTTCAT GAAAAACTAT GATGGGGTAG CTGCTGCTTC TTTCTCACGA 
GCTGTGGAAA CTGTTGAAGC CAATGTGCGC TGGAAAATGC TTTACCAAGA CGAGCTTTTC 
CAATGGTTAG GAAAAGCTCT AAGACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRHDE ( XM_016923853.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016779342.1 Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc66225
Clone ID Related Accession (Same CDS sequence)	XM_016923853.1 , XM_016923853.2
Accession Version	XM_016923853.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	thyrotropin-releasing hormone-degrading ectoenzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016923853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG ACGGCGAGCT GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAAAAG 
AAGAGGAAGA AGAAGAAGAA GAAGGAGGAG GAGGAGGAGG GGGCCGAGAA GAGCAGCTCA 
CCCTTTGCAG CCGCGATGGG GGAAGACGAC GCCGCGCTTC GGGCTGGCGG CAGGGGGCTC 
TCCGACCCGT GGGCAGACTC AGTGGGAGTG CGACCCCGCA CCACGGAGCG CCACATCGCC 
GTACACAAGC GGCTTGTGCT GGCCTTCGCC GTGTCCCTCG TGGCATTGCT CGCGGTCACA 
ATGCTCGCCG TGCTGCTCAG CCTGCGCTTC GACGAGTGCG GGGCGAGTGC CACGCCAGGC 
GCCGACGGTG GCCCCTCAGG CTTTCCGGAG CGCGGCGGCA ACGGGAGCCT CCCTGGATCG 
GCCCGGCGCA ACCACCACGC AGGCGGGGAC TCCTGGCAGC CCGAGGCGGG TGGGGTGGCC 
AGTCCGGGGA CCCCGTCGGC CCAGCCGCCG TCGGAGGAGG AGCGGGAGCC GTGGGAGCCG 
TGGACGCAGC TGCGCCTGTC GGGCCACCTG AAGCCGCTGC ACTACAATCT GATGCTCACC 
GCCTTCATGG AGAACTTCAC CTTCTCCGGG GAGGTCAACG TGGAGATCGC GTGCCGGAAC 
GCCACCCGCT ACGTAGTGCT GCACGCTTCC CGAGTGGCGG TGGAGAAAGT GCAGCTGGCC 
GAGGACCGGG CGTTCGGGGC TGTCCCTGTG GCCGGTTTTT TCCTCTACCC GCAAACCCAG 
GTCTTAGTGG TGGTGCTGAA TAGGACACTG GACGCGCAGA GGAATTACAA TCTGAAGATT 
ATCTATAACG CGCTCATCGA GAATGAGCTC CTGGGCTTCT TCCGCAGCTC CTATGTGCTC 
CACGGGGAGA GAAGATTCCT TGGTGTTACT CAGTTTTCGC CTACACATGC CAGAAAGGCA 
TTTCCTTGTT TTGATGAGCC AATCTACAAG GCTACTTTCA AAATCAGCAT CAAGCATCAA 
GCAACCTATT TATCTTTATC TAATATGCCA GTGGAAACTT CCGTGTTTGA GGAAGATGGA 
TGGGTTACGG ATCACTTTTC ACAGACCCCT CTCATGTCCA CATATTATTT AGCCTGGGCA 
ATTTGCAACT TCACATACAG AGAAACTACC ACCAAGAGTG GGGTTGTAGT ACGATTATAT 
GCAAGACCTG ATGCTATCAG AAGAGGATCC GGGGACTATG CTCTCCATAT AACAAAGAGA 
TTAATAGAAT TTTATGAAGA CTACTTTAAA GTGCCCTATT CCTTGCCAAA ACTAGATCTT 
TTAGCTGTGC CTAAGCATCC GTATGCTGCT ATGGAGAACT GGGGACTAAG TATTTTTGTG 
GAACAAAGAA TACTGCTGGA TCCCAGTGTT TCATCTATTT CTTATTTGCT GGATGTCACC 
ATGGTCATTG TTCATGAGAT ATGTCACCAG TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG 
TGGGAAGACG TGTGGCTGAA GGAAGGGTTT GCTCACTACT TTGAATTTGT TGGTACAGAC 
TACCTCTATC CTGGCTGGAA CATGGAAAAG CAGAGGTTTC TGACCGATGT TCTGCATGAA 
GTGATGCTGC TGGACGGTTT GGCCAGTTCC CATCCAGTAT CACAGGAAGT GCTGCAGGCA 
ACAGATATTG ACAGGGTGTT TGACTGGATC GCATATAAAA AGGGTGCTGC TTTAATAAGA 
ATGCTGGCTA ATTTTATGGG CCATTCAGTT TTCCAGAGGG GTTTGCAAGA TTATTTAACC 
ATTCATAAGT ATGGCAATGC AGCCAGAAAT GATCTCTGGA ATACATTATC GGAGGCATTA 
AAAAGAAATG GGAAATATGT AAATATACAA GAAGTAATGG ATCAGTGGAC ACTCCAGATG 
GGTTATCCTG TTATCACCAT CTTGGGAAAC ACAACAGCAG AAAATAGAAT AATAATTACC 
CAACAGCATT TTATCTATGA TATCAGTGCT AAAACTAAAG CACTTAAACT TCAGAATAAC 
AGTTACCTGT GGCAGATTCC ATTAACTATT GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA 
GAAGCAATTA TTTGGGTGTC TAACAAATCA GAGCACCACA GAATAACTTA TTTGGACAAA 
GGAAGCTGGC TGCTGGGGAA CATCAATCAA ACTGGCTATT TTAGAGTCAA CTATGACCTA 
AGGAACTGGA GATTATTAAT TGATCAATTA ATCCGGAATC ATGAGGTTCT TTCTGTCAGT 
AACCGAGCGG GCTTGATCGA TGATGCCTTC AGCCTAGCCA GGGCTGGCTA TTTGCCTCAG 
AATATTCCTC TGGAGATTAT CAGATACCTG TCTGAGGAGA AGGATTTTCT TCCTTGGCAT 
GCTGCCAGCC GAGCTCTTTA TCCTCTAGAT AAATTACTGG ACCGCATGGA AAACTACAAC 
ATTTTCAATG AATATATCTT AAAGCAAGTT GCAACAACGT ATATCAAGCT TGGGTGGCCG 
AAAAATAATT TTAATGGATC TCTTGTTCAA GCATCCTACC AACATGAAGA ACTACGTAGA 
GAAGTTATAA TGCTGGCCTG CAGTTTTGGC AACAAGCACT GTCACCAACA GGCATCAACA 
CTTATTTCAG ATTGGATTTC CAGCAACAGG AACAGAATAC CACTAAATGT TAGAGACATC 
GTATACTGTA CAGGAGTGTC ACTGCTGGAT GAGGATGTCT GGGAATTCAT ATGGATGAAA 
TTCCATTCCA CCACAGCAGT TTCTGAGAAG AAAATATTAT TGGAAGCCTT AACTTGCAGT 
GATGACAGGA ATTTATTAAA CAGGCTTCTA AATCTGTCAC TGAATTCTGA GGTGGTGCTG 
GATCAAGATG CAATTGATGT CATAATCCAT GTAGCTCGAA ATCCACATGG TCGAGACCTT 
GCCTGGAAGT TTTTCAGGGA TAAATGGAAG ATATTAAATA CCAGGTATGG AGAAGCATTG 
TTTATGAATT CCAAACTCAT CAGTGGTGTC ACAGAATTTC TTAATACTGA AGGTGAACTC 
AAAGAGCTCA AGAACTTCAT GAAAAACTAT GATGGGGTAG CTGCTGCTTC TTTCTCACGA 
GCTGTGGAAA CTGTTGAAGC CAATGTGCGC TGGAAAATGC TTTACCAAGA CGAGCTTTTC 
CAATGGTTAG GAAAAGCTCT AAGACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016779342.1
CDS	122..3331
Translation	MALDGELGEQEEEKKKKKKKKRKKKKKKEEEEEGAEKSSSPFAAAMGEDDAALRAGGRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSLVALLAVTMLAVLLSLRFDECGASATPGADGGPSGFPERGGNGSLPGSARRNHHAGGDSWQPEAGGVASPGTPSAQPPSEEEREPWEPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQLAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRNYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKALKLQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNIFNEYILKQVATTYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLNRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTRYGEALFMNSKLISGVTEFLNTEGELKELKNFMKNYDGVAAASFSRAVETVEANVRWKMLYQDELFQWLGKALRH

Target ORF information:

RefSeq Version	XM_016923853.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016923853.2

ORF Insert Sequence:

ATGGCCCTGG ACGGCGAGCT GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAAAAG 
AAGAGGAAGA AGAAGAAGAA GAAGGAGGAG GAGGAGGAGG GGGCCGAGAA GAGCAGCTCA 
CCCTTTGCAG CCGCGATGGG GGAAGACGAC GCCGCGCTTC GGGCTGGCGG CAGGGGGCTC 
TCCGACCCGT GGGCAGACTC AGTGGGAGTG CGACCCCGCA CCACGGAGCG CCACATCGCC 
GTACACAAGC GGCTTGTGCT GGCCTTCGCC GTGTCCCTCG TGGCATTGCT CGCGGTCACA 
ATGCTCGCCG TGCTGCTCAG CCTGCGCTTC GACGAGTGCG GGGCGAGTGC CACGCCAGGC 
GCCGACGGTG GCCCCTCAGG CTTTCCGGAG CGCGGCGGCA ACGGGAGCCT CCCTGGATCG 
GCCCGGCGCA ACCACCACGC AGGCGGGGAC TCCTGGCAGC CCGAGGCGGG TGGGGTGGCC 
AGTCCGGGGA CCCCGTCGGC CCAGCCGCCG TCGGAGGAGG AGCGGGAGCC GTGGGAGCCG 
TGGACGCAGC TGCGCCTGTC GGGCCACCTG AAGCCGCTGC ACTACAATCT GATGCTCACC 
GCCTTCATGG AGAACTTCAC CTTCTCCGGG GAGGTCAACG TGGAGATCGC GTGCCGGAAC 
GCCACCCGCT ACGTAGTGCT GCACGCTTCC CGAGTGGCGG TGGAGAAAGT GCAGCTGGCC 
GAGGACCGGG CGTTCGGGGC TGTCCCTGTG GCCGGTTTTT TCCTCTACCC GCAAACCCAG 
GTCTTAGTGG TGGTGCTGAA TAGGACACTG GACGCGCAGA GGAATTACAA TCTGAAGATT 
ATCTATAACG CGCTCATCGA GAATGAGCTC CTGGGCTTCT TCCGCAGCTC CTATGTGCTC 
CACGGGGAGA GAAGATTCCT TGGTGTTACT CAGTTTTCGC CTACACATGC CAGAAAGGCA 
TTTCCTTGTT TTGATGAGCC AATCTACAAG GCTACTTTCA AAATCAGCAT CAAGCATCAA 
GCAACCTATT TATCTTTATC TAATATGCCA GTGGAAACTT CCGTGTTTGA GGAAGATGGA 
TGGGTTACGG ATCACTTTTC ACAGACCCCT CTCATGTCCA CATATTATTT AGCCTGGGCA 
ATTTGCAACT TCACATACAG AGAAACTACC ACCAAGAGTG GGGTTGTAGT ACGATTATAT 
GCAAGACCTG ATGCTATCAG AAGAGGATCC GGGGACTATG CTCTCCATAT AACAAAGAGA 
TTAATAGAAT TTTATGAAGA CTACTTTAAA GTGCCCTATT CCTTGCCAAA ACTAGATCTT 
TTAGCTGTGC CTAAGCATCC GTATGCTGCT ATGGAGAACT GGGGACTAAG TATTTTTGTG 
GAACAAAGAA TACTGCTGGA TCCCAGTGTT TCATCTATTT CTTATTTGCT GGATGTCACC 
ATGGTCATTG TTCATGAGAT ATGTCACCAG TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG 
TGGGAAGACG TGTGGCTGAA GGAAGGGTTT GCTCACTACT TTGAATTTGT TGGTACAGAC 
TACCTCTATC CTGGCTGGAA CATGGAAAAG CAGAGGTTTC TGACCGATGT TCTGCATGAA 
GTGATGCTGC TGGACGGTTT GGCCAGTTCC CATCCAGTAT CACAGGAAGT GCTGCAGGCA 
ACAGATATTG ACAGGGTGTT TGACTGGATC GCATATAAAA AGGGTGCTGC TTTAATAAGA 
ATGCTGGCTA ATTTTATGGG CCATTCAGTT TTCCAGAGGG GTTTGCAAGA TTATTTAACC 
ATTCATAAGT ATGGCAATGC AGCCAGAAAT GATCTCTGGA ATACATTATC GGAGGCATTA 
AAAAGAAATG GGAAATATGT AAATATACAA GAAGTAATGG ATCAGTGGAC ACTCCAGATG 
GGTTATCCTG TTATCACCAT CTTGGGAAAC ACAACAGCAG AAAATAGAAT AATAATTACC 
CAACAGCATT TTATCTATGA TATCAGTGCT AAAACTAAAG CACTTAAACT TCAGAATAAC 
AGTTACCTGT GGCAGATTCC ATTAACTATT GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA 
GAAGCAATTA TTTGGGTGTC TAACAAATCA GAGCACCACA GAATAACTTA TTTGGACAAA 
GGAAGCTGGC TGCTGGGGAA CATCAATCAA ACTGGCTATT TTAGAGTCAA CTATGACCTA 
AGGAACTGGA GATTATTAAT TGATCAATTA ATCCGGAATC ATGAGGTTCT TTCTGTCAGT 
AACCGAGCGG GCTTGATCGA TGATGCCTTC AGCCTAGCCA GGGCTGGCTA TTTGCCTCAG 
AATATTCCTC TGGAGATTAT CAGATACCTG TCTGAGGAGA AGGATTTTCT TCCTTGGCAT 
GCTGCCAGCC GAGCTCTTTA TCCTCTAGAT AAATTACTGG ACCGCATGGA AAACTACAAC 
ATTTTCAATG AATATATCTT AAAGCAAGTT GCAACAACGT ATATCAAGCT TGGGTGGCCG 
AAAAATAATT TTAATGGATC TCTTGTTCAA GCATCCTACC AACATGAAGA ACTACGTAGA 
GAAGTTATAA TGCTGGCCTG CAGTTTTGGC AACAAGCACT GTCACCAACA GGCATCAACA 
CTTATTTCAG ATTGGATTTC CAGCAACAGG AACAGAATAC CACTAAATGT TAGAGACATC 
GTATACTGTA CAGGAGTGTC ACTGCTGGAT GAGGATGTCT GGGAATTCAT ATGGATGAAA 
TTCCATTCCA CCACAGCAGT TTCTGAGAAG AAAATATTAT TGGAAGCCTT AACTTGCAGT 
GATGACAGGA ATTTATTAAA CAGGCTTCTA AATCTGTCAC TGAATTCTGA GGTGGTGCTG 
GATCAAGATG CAATTGATGT CATAATCCAT GTAGCTCGAA ATCCACATGG TCGAGACCTT 
GCCTGGAAGT TTTTCAGGGA TAAATGGAAG ATATTAAATA CCAGGTATGG AGAAGCATTG 
TTTATGAATT CCAAACTCAT CAGTGGTGTC ACAGAATTTC TTAATACTGA AGGTGAACTC 
AAAGAGCTCA AGAACTTCAT GAAAAACTAT GATGGGGTAG CTGCTGCTTC TTTCTCACGA 
GCTGTGGAAA CTGTTGAAGC CAATGTGCGC TGGAAAATGC TTTACCAAGA CGAGCTTTTC 
CAATGGTTAG GAAAAGCTCT AAGACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRHDE ( XM_016923854.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016779343.1 Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc66226
Clone ID Related Accession (Same CDS sequence)	XM_016923854.1 , XM_016923854.2
Accession Version	XM_016923854.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	thyrotropin-releasing hormone-degrading ectoenzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006479.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG ACGGCGAGCT GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAAAAG 
AAGAGGAAGA AGAAGAAGAA GAAGGAGGAG GAGGAGGAGG GGGCCGAGAA GAGCAGCTCA 
CCCTTTGCAG CCGCGATGGG GGAAGACGAC GCCGCGCTTC GGGCTGGCGG CAGGGGGCTC 
TCCGACCCGT GGGCAGACTC AGTGGGAGTG CGACCCCGCA CCACGGAGCG CCACATCGCC 
GTACACAAGC GGCTTGTGCT GGCCTTCGCC GTGTCCCTCG TGGCATTGCT CGCGGTCACA 
ATGCTCGCCG TGCTGCTCAG CCTGCGCTTC GACGAGTGCG GGGCGAGTGC CACGCCAGGC 
GCCGACGGTG GCCCCTCAGG CTTTCCGGAG CGCGGCGGCA ACGGGAGCCT CCCTGGATCG 
GCCCGGCGCA ACCACCACGC AGGCGGGGAC TCCTGGCAGC CCGAGGCGGG TGGGGTGGCC 
AGTCCGGGGA CCCCGTCGGC CCAGCCGCCG TCGGAGGAGG AGCGGGAGCC GTGGGAGCCG 
TGGACGCAGC TGCGCCTGTC GGGCCACCTG AAGCCGCTGC ACTACAATCT GATGCTCACC 
GCCTTCATGG AGAACTTCAC CTTCTCCGGG GAGGTCAACG TGGAGATCGC GTGCCGGAAC 
GCCACCCGCT ACGTAGTGCT GCACGCTTCC CGAGTGGCGG TGGAGAAAGT GCAGCTGGCC 
GAGGACCGGG CGTTCGGGGC TGTCCCTGTG GCCGGTTTTT TCCTCTACCC GCAAACCCAG 
GTCTTAGTGG TGGTGCTGAA TAGGACACTG GACGCGCAGA GGAATTACAA TCTGAAGATT 
ATCTATAACG CGCTCATCGA GAATGAGCTC CTGGGCTTCT TCCGCAGCTC CTATGTGCTC 
CACGGGGAGA GAAGATTCCT TGGTGTTACT CAGTTTTCGC CTACACATGC CAGAAAGGCA 
TTTCCTTGTT TTGATGAGCC AATCTACAAG GCTACTTTCA AAATCAGCAT CAAGCATCAA 
GCAACCTATT TATCTTTATC TAATATGCCA GTGGAAACTT CCGTGTTTGA GGAAGATGGA 
TGGGTTACGG ATCACTTTTC ACAGACCCCT CTCATGTCCA CATATTATTT AGCCTGGGCA 
ATTTGCAACT TCACATACAG AGAAACTACC ACCAAGAGTG GGGTTGTAGT ACGATTATAT 
GCAAGACCTG ATGCTATCAG AAGAGGATCC GGGGACTATG CTCTCCATAT AACAAAGAGA 
TTAATAGAAT TTTATGAAGA CTACTTTAAA GTGCCCTATT CCTTGCCAAA ACTAGATCTT 
TTAGCTGTGC CTAAGCATCC GTATGCTGCT ATGGAGAACT GGGGACTAAG TATTTTTGTG 
GAACAAAGAA TACTGCTGGA TCCCAGTGTT TCATCTATTT CTTATTTGCT GGATGTCACC 
ATGGTCATTG TTCATGAGAT ATGTCACCAG TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG 
TGGGAAGACG TGTGGCTGAA GGAAGGGTTT GCTCACTACT TTGAATTTGT TGGTACAGAC 
TACCTCTATC CTGGCTGGAA CATGGAAAAG CAGAGGTTTC TGACCGATGT TCTGCATGAA 
GTGATGCTGC TGGACGGTTT GGCCAGTTCC CATCCAGTAT CACAGGAAGT GCTGCAGGCA 
ACAGATATTG ACAGGGTGTT TGACTGGATC GCATATAAAA AGGGTGCTGC TTTAATAAGA 
ATGCTGGCTA ATTTTATGGG CCATTCAGTT TTCCAGAGGG GTTTGCAAGA TTATTTAACC 
ATTCATAAGT ATGGCAATGC AGCCAGAAAT GATCTCTGGA ATACATTATC GGAGGCATTA 
AAAAGAAATG GGAAATATGT AAATATACAA GAAGTAATGG ATCAGTGGAC ACTCCAGATG 
GGTTATCCTG TTATCACCAT CTTGGGAAAC ACAACAGCAG AAAATAGAAT AATAATTACC 
CAACAGCATT TTATCTATGA TATCAGTGCT AAAACTAAAG CACTTAAACT TCAGAATAAC 
AGTTACCTGT GGCAGATTCC ATTAACTATT GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA 
GAAGCAATTA TTTGGGTGTC TAACAAATCA GAGCACCACA GAATAACTTA TTTGGACAAA 
GGAAGCTGGC TGCTGGGGAA CATCAATCAA ACTGGCTATT TTAGAGTCAA CTATGACCTA 
AGGAACTGGA GATTATTAAT TGATCAATTA ATCCGGAATC ATGAGGTTCT TTCTGTCAGT 
AACCGAGCGG GCTTGATCGA TGATGCCTTC AGCCTAGCCA GGGCTGGCTA TTTGCCTCAG 
AATATTCCTC TGGAGATTAT CAGATACCTG TCTGAGGAGA AGGATTTTCT TCCTTGGCAT 
GCTGCCAGCC GAGCTCTTTA TCCTCTAGAT AAATTACTGG ACCGCATGGA AAACTACAAC 
ATTTTCAATG AATATATCTT AAAGCAAGTT GCAACAACGT ATATCAAGCT TGGGTGGCCG 
AAAAATAATT TTAATGGATC TCTTGTTCAA GCATCCTACC AACATGAAGA ACTACGTAGA 
GAAGTTATAA TGCTGGCCTG CAGTTTTGGC AACAAGCACT GTCACCAACA GGCATCAACA 
CTTATTTCAG ATTGGATTTC CAGCAACAGG AACAGAATAC CACTAAATGT TAGAGACATC 
GTATACTGTA CAGGAGTGTC ACTGCTGGAT GAGGATGTCT GGGAATTCAT ATGGATGAAA 
TTCCATTCCA CCACAGCAGT TTCTGAGAAG AAAATATTAT TGGAAGCCTT AACTTGCAGT 
GATGACAGGA ATTTATTAAA CAGGCTTCTA AATCTGTCAC TGAATTCTGA GGTGGTGCTG 
GATCAAGATG CAATTGATGT CATAATCCAT GTAGCTCGAA ATCCACATGG TCGAGACCTT 
GCCTGGAAGT TTTTCAGGGA TAAATGGAAG ATATTAAATA CCAGTTGCAG TAAAATTTAC 
TCCCAGGACA TCTTGTTTCA TGGTGAGAAT TCTTTAGCCT CAATTTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016779343.1
CDS	221..3271
Translation	MALDGELGEQEEEKKKKKKKKRKKKKKKEEEEEGAEKSSSPFAAAMGEDDAALRAGGRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSLVALLAVTMLAVLLSLRFDECGASATPGADGGPSGFPERGGNGSLPGSARRNHHAGGDSWQPEAGGVASPGTPSAQPPSEEEREPWEPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQLAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRNYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKALKLQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNIFNEYILKQVATTYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLNRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTSCSKIYSQDILFHGENSLASIL

Target ORF information:

RefSeq Version	XM_016923854.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016923854.1

ORF Insert Sequence:

ATGGCCCTGG ACGGCGAGCT GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAAAAG 
AAGAGGAAGA AGAAGAAGAA GAAGGAGGAG GAGGAGGAGG GGGCCGAGAA GAGCAGCTCA 
CCCTTTGCAG CCGCGATGGG GGAAGACGAC GCCGCGCTTC GGGCTGGCGG CAGGGGGCTC 
TCCGACCCGT GGGCAGACTC AGTGGGAGTG CGACCCCGCA CCACGGAGCG CCACATCGCC 
GTACACAAGC GGCTTGTGCT GGCCTTCGCC GTGTCCCTCG TGGCATTGCT CGCGGTCACA 
ATGCTCGCCG TGCTGCTCAG CCTGCGCTTC GACGAGTGCG GGGCGAGTGC CACGCCAGGC 
GCCGACGGTG GCCCCTCAGG CTTTCCGGAG CGCGGCGGCA ACGGGAGCCT CCCTGGATCG 
GCCCGGCGCA ACCACCACGC AGGCGGGGAC TCCTGGCAGC CCGAGGCGGG TGGGGTGGCC 
AGTCCGGGGA CCCCGTCGGC CCAGCCGCCG TCGGAGGAGG AGCGGGAGCC GTGGGAGCCG 
TGGACGCAGC TGCGCCTGTC GGGCCACCTG AAGCCGCTGC ACTACAATCT GATGCTCACC 
GCCTTCATGG AGAACTTCAC CTTCTCCGGG GAGGTCAACG TGGAGATCGC GTGCCGGAAC 
GCCACCCGCT ACGTAGTGCT GCACGCTTCC CGAGTGGCGG TGGAGAAAGT GCAGCTGGCC 
GAGGACCGGG CGTTCGGGGC TGTCCCTGTG GCCGGTTTTT TCCTCTACCC GCAAACCCAG 
GTCTTAGTGG TGGTGCTGAA TAGGACACTG GACGCGCAGA GGAATTACAA TCTGAAGATT 
ATCTATAACG CGCTCATCGA GAATGAGCTC CTGGGCTTCT TCCGCAGCTC CTATGTGCTC 
CACGGGGAGA GAAGATTCCT TGGTGTTACT CAGTTTTCGC CTACACATGC CAGAAAGGCA 
TTTCCTTGTT TTGATGAGCC AATCTACAAG GCTACTTTCA AAATCAGCAT CAAGCATCAA 
GCAACCTATT TATCTTTATC TAATATGCCA GTGGAAACTT CCGTGTTTGA GGAAGATGGA 
TGGGTTACGG ATCACTTTTC ACAGACCCCT CTCATGTCCA CATATTATTT AGCCTGGGCA 
ATTTGCAACT TCACATACAG AGAAACTACC ACCAAGAGTG GGGTTGTAGT ACGATTATAT 
GCAAGACCTG ATGCTATCAG AAGAGGATCC GGGGACTATG CTCTCCATAT AACAAAGAGA 
TTAATAGAAT TTTATGAAGA CTACTTTAAA GTGCCCTATT CCTTGCCAAA ACTAGATCTT 
TTAGCTGTGC CTAAGCATCC GTATGCTGCT ATGGAGAACT GGGGACTAAG TATTTTTGTG 
GAACAAAGAA TACTGCTGGA TCCCAGTGTT TCATCTATTT CTTATTTGCT GGATGTCACC 
ATGGTCATTG TTCATGAGAT ATGTCACCAG TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG 
TGGGAAGACG TGTGGCTGAA GGAAGGGTTT GCTCACTACT TTGAATTTGT TGGTACAGAC 
TACCTCTATC CTGGCTGGAA CATGGAAAAG CAGAGGTTTC TGACCGATGT TCTGCATGAA 
GTGATGCTGC TGGACGGTTT GGCCAGTTCC CATCCAGTAT CACAGGAAGT GCTGCAGGCA 
ACAGATATTG ACAGGGTGTT TGACTGGATC GCATATAAAA AGGGTGCTGC TTTAATAAGA 
ATGCTGGCTA ATTTTATGGG CCATTCAGTT TTCCAGAGGG GTTTGCAAGA TTATTTAACC 
ATTCATAAGT ATGGCAATGC AGCCAGAAAT GATCTCTGGA ATACATTATC GGAGGCATTA 
AAAAGAAATG GGAAATATGT AAATATACAA GAAGTAATGG ATCAGTGGAC ACTCCAGATG 
GGTTATCCTG TTATCACCAT CTTGGGAAAC ACAACAGCAG AAAATAGAAT AATAATTACC 
CAACAGCATT TTATCTATGA TATCAGTGCT AAAACTAAAG CACTTAAACT TCAGAATAAC 
AGTTACCTGT GGCAGATTCC ATTAACTATT GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA 
GAAGCAATTA TTTGGGTGTC TAACAAATCA GAGCACCACA GAATAACTTA TTTGGACAAA 
GGAAGCTGGC TGCTGGGGAA CATCAATCAA ACTGGCTATT TTAGAGTCAA CTATGACCTA 
AGGAACTGGA GATTATTAAT TGATCAATTA ATCCGGAATC ATGAGGTTCT TTCTGTCAGT 
AACCGAGCGG GCTTGATCGA TGATGCCTTC AGCCTAGCCA GGGCTGGCTA TTTGCCTCAG 
AATATTCCTC TGGAGATTAT CAGATACCTG TCTGAGGAGA AGGATTTTCT TCCTTGGCAT 
GCTGCCAGCC GAGCTCTTTA TCCTCTAGAT AAATTACTGG ACCGCATGGA AAACTACAAC 
ATTTTCAATG AATATATCTT AAAGCAAGTT GCAACAACGT ATATCAAGCT TGGGTGGCCG 
AAAAATAATT TTAATGGATC TCTTGTTCAA GCATCCTACC AACATGAAGA ACTACGTAGA 
GAAGTTATAA TGCTGGCCTG CAGTTTTGGC AACAAGCACT GTCACCAACA GGCATCAACA 
CTTATTTCAG ATTGGATTTC CAGCAACAGG AACAGAATAC CACTAAATGT TAGAGACATC 
GTATACTGTA CAGGAGTGTC ACTGCTGGAT GAGGATGTCT GGGAATTCAT ATGGATGAAA 
TTCCATTCCA CCACAGCAGT TTCTGAGAAG AAAATATTAT TGGAAGCCTT AACTTGCAGT 
GATGACAGGA ATTTATTAAA CAGGCTTCTA AATCTGTCAC TGAATTCTGA GGTGGTGCTG 
GATCAAGATG CAATTGATGT CATAATCCAT GTAGCTCGAA ATCCACATGG TCGAGACCTT 
GCCTGGAAGT TTTTCAGGGA TAAATGGAAG ATATTAAATA CCAGTTGCAG TAAAATTTAC 
TCCCAGGACA TCTTGTTTCA TGGTGAGAAT TCTTTAGCCT CAATTTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRHDE ( XM_016923854.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016779343.1 Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc66226
Clone ID Related Accession (Same CDS sequence)	XM_016923854.1 , XM_016923854.2
Accession Version	XM_016923854.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	thyrotropin-releasing hormone-degrading ectoenzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016923854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG ACGGCGAGCT GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAAAAG 
AAGAGGAAGA AGAAGAAGAA GAAGGAGGAG GAGGAGGAGG GGGCCGAGAA GAGCAGCTCA 
CCCTTTGCAG CCGCGATGGG GGAAGACGAC GCCGCGCTTC GGGCTGGCGG CAGGGGGCTC 
TCCGACCCGT GGGCAGACTC AGTGGGAGTG CGACCCCGCA CCACGGAGCG CCACATCGCC 
GTACACAAGC GGCTTGTGCT GGCCTTCGCC GTGTCCCTCG TGGCATTGCT CGCGGTCACA 
ATGCTCGCCG TGCTGCTCAG CCTGCGCTTC GACGAGTGCG GGGCGAGTGC CACGCCAGGC 
GCCGACGGTG GCCCCTCAGG CTTTCCGGAG CGCGGCGGCA ACGGGAGCCT CCCTGGATCG 
GCCCGGCGCA ACCACCACGC AGGCGGGGAC TCCTGGCAGC CCGAGGCGGG TGGGGTGGCC 
AGTCCGGGGA CCCCGTCGGC CCAGCCGCCG TCGGAGGAGG AGCGGGAGCC GTGGGAGCCG 
TGGACGCAGC TGCGCCTGTC GGGCCACCTG AAGCCGCTGC ACTACAATCT GATGCTCACC 
GCCTTCATGG AGAACTTCAC CTTCTCCGGG GAGGTCAACG TGGAGATCGC GTGCCGGAAC 
GCCACCCGCT ACGTAGTGCT GCACGCTTCC CGAGTGGCGG TGGAGAAAGT GCAGCTGGCC 
GAGGACCGGG CGTTCGGGGC TGTCCCTGTG GCCGGTTTTT TCCTCTACCC GCAAACCCAG 
GTCTTAGTGG TGGTGCTGAA TAGGACACTG GACGCGCAGA GGAATTACAA TCTGAAGATT 
ATCTATAACG CGCTCATCGA GAATGAGCTC CTGGGCTTCT TCCGCAGCTC CTATGTGCTC 
CACGGGGAGA GAAGATTCCT TGGTGTTACT CAGTTTTCGC CTACACATGC CAGAAAGGCA 
TTTCCTTGTT TTGATGAGCC AATCTACAAG GCTACTTTCA AAATCAGCAT CAAGCATCAA 
GCAACCTATT TATCTTTATC TAATATGCCA GTGGAAACTT CCGTGTTTGA GGAAGATGGA 
TGGGTTACGG ATCACTTTTC ACAGACCCCT CTCATGTCCA CATATTATTT AGCCTGGGCA 
ATTTGCAACT TCACATACAG AGAAACTACC ACCAAGAGTG GGGTTGTAGT ACGATTATAT 
GCAAGACCTG ATGCTATCAG AAGAGGATCC GGGGACTATG CTCTCCATAT AACAAAGAGA 
TTAATAGAAT TTTATGAAGA CTACTTTAAA GTGCCCTATT CCTTGCCAAA ACTAGATCTT 
TTAGCTGTGC CTAAGCATCC GTATGCTGCT ATGGAGAACT GGGGACTAAG TATTTTTGTG 
GAACAAAGAA TACTGCTGGA TCCCAGTGTT TCATCTATTT CTTATTTGCT GGATGTCACC 
ATGGTCATTG TTCATGAGAT ATGTCACCAG TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG 
TGGGAAGACG TGTGGCTGAA GGAAGGGTTT GCTCACTACT TTGAATTTGT TGGTACAGAC 
TACCTCTATC CTGGCTGGAA CATGGAAAAG CAGAGGTTTC TGACCGATGT TCTGCATGAA 
GTGATGCTGC TGGACGGTTT GGCCAGTTCC CATCCAGTAT CACAGGAAGT GCTGCAGGCA 
ACAGATATTG ACAGGGTGTT TGACTGGATC GCATATAAAA AGGGTGCTGC TTTAATAAGA 
ATGCTGGCTA ATTTTATGGG CCATTCAGTT TTCCAGAGGG GTTTGCAAGA TTATTTAACC 
ATTCATAAGT ATGGCAATGC AGCCAGAAAT GATCTCTGGA ATACATTATC GGAGGCATTA 
AAAAGAAATG GGAAATATGT AAATATACAA GAAGTAATGG ATCAGTGGAC ACTCCAGATG 
GGTTATCCTG TTATCACCAT CTTGGGAAAC ACAACAGCAG AAAATAGAAT AATAATTACC 
CAACAGCATT TTATCTATGA TATCAGTGCT AAAACTAAAG CACTTAAACT TCAGAATAAC 
AGTTACCTGT GGCAGATTCC ATTAACTATT GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA 
GAAGCAATTA TTTGGGTGTC TAACAAATCA GAGCACCACA GAATAACTTA TTTGGACAAA 
GGAAGCTGGC TGCTGGGGAA CATCAATCAA ACTGGCTATT TTAGAGTCAA CTATGACCTA 
AGGAACTGGA GATTATTAAT TGATCAATTA ATCCGGAATC ATGAGGTTCT TTCTGTCAGT 
AACCGAGCGG GCTTGATCGA TGATGCCTTC AGCCTAGCCA GGGCTGGCTA TTTGCCTCAG 
AATATTCCTC TGGAGATTAT CAGATACCTG TCTGAGGAGA AGGATTTTCT TCCTTGGCAT 
GCTGCCAGCC GAGCTCTTTA TCCTCTAGAT AAATTACTGG ACCGCATGGA AAACTACAAC 
ATTTTCAATG AATATATCTT AAAGCAAGTT GCAACAACGT ATATCAAGCT TGGGTGGCCG 
AAAAATAATT TTAATGGATC TCTTGTTCAA GCATCCTACC AACATGAAGA ACTACGTAGA 
GAAGTTATAA TGCTGGCCTG CAGTTTTGGC AACAAGCACT GTCACCAACA GGCATCAACA 
CTTATTTCAG ATTGGATTTC CAGCAACAGG AACAGAATAC CACTAAATGT TAGAGACATC 
GTATACTGTA CAGGAGTGTC ACTGCTGGAT GAGGATGTCT GGGAATTCAT ATGGATGAAA 
TTCCATTCCA CCACAGCAGT TTCTGAGAAG AAAATATTAT TGGAAGCCTT AACTTGCAGT 
GATGACAGGA ATTTATTAAA CAGGCTTCTA AATCTGTCAC TGAATTCTGA GGTGGTGCTG 
GATCAAGATG CAATTGATGT CATAATCCAT GTAGCTCGAA ATCCACATGG TCGAGACCTT 
GCCTGGAAGT TTTTCAGGGA TAAATGGAAG ATATTAAATA CCAGTTGCAG TAAAATTTAC 
TCCCAGGACA TCTTGTTTCA TGGTGAGAAT TCTTTAGCCT CAATTTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016779343.1
CDS	123..3173
Translation	MALDGELGEQEEEKKKKKKKKRKKKKKKEEEEEGAEKSSSPFAAAMGEDDAALRAGGRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSLVALLAVTMLAVLLSLRFDECGASATPGADGGPSGFPERGGNGSLPGSARRNHHAGGDSWQPEAGGVASPGTPSAQPPSEEEREPWEPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQLAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRNYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKALKLQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNIFNEYILKQVATTYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLNRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTSCSKIYSQDILFHGENSLASIL

Target ORF information:

RefSeq Version	XM_016923854.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016923854.2

ORF Insert Sequence:

ATGGCCCTGG ACGGCGAGCT GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAAAAG 
AAGAGGAAGA AGAAGAAGAA GAAGGAGGAG GAGGAGGAGG GGGCCGAGAA GAGCAGCTCA 
CCCTTTGCAG CCGCGATGGG GGAAGACGAC GCCGCGCTTC GGGCTGGCGG CAGGGGGCTC 
TCCGACCCGT GGGCAGACTC AGTGGGAGTG CGACCCCGCA CCACGGAGCG CCACATCGCC 
GTACACAAGC GGCTTGTGCT GGCCTTCGCC GTGTCCCTCG TGGCATTGCT CGCGGTCACA 
ATGCTCGCCG TGCTGCTCAG CCTGCGCTTC GACGAGTGCG GGGCGAGTGC CACGCCAGGC 
GCCGACGGTG GCCCCTCAGG CTTTCCGGAG CGCGGCGGCA ACGGGAGCCT CCCTGGATCG 
GCCCGGCGCA ACCACCACGC AGGCGGGGAC TCCTGGCAGC CCGAGGCGGG TGGGGTGGCC 
AGTCCGGGGA CCCCGTCGGC CCAGCCGCCG TCGGAGGAGG AGCGGGAGCC GTGGGAGCCG 
TGGACGCAGC TGCGCCTGTC GGGCCACCTG AAGCCGCTGC ACTACAATCT GATGCTCACC 
GCCTTCATGG AGAACTTCAC CTTCTCCGGG GAGGTCAACG TGGAGATCGC GTGCCGGAAC 
GCCACCCGCT ACGTAGTGCT GCACGCTTCC CGAGTGGCGG TGGAGAAAGT GCAGCTGGCC 
GAGGACCGGG CGTTCGGGGC TGTCCCTGTG GCCGGTTTTT TCCTCTACCC GCAAACCCAG 
GTCTTAGTGG TGGTGCTGAA TAGGACACTG GACGCGCAGA GGAATTACAA TCTGAAGATT 
ATCTATAACG CGCTCATCGA GAATGAGCTC CTGGGCTTCT TCCGCAGCTC CTATGTGCTC 
CACGGGGAGA GAAGATTCCT TGGTGTTACT CAGTTTTCGC CTACACATGC CAGAAAGGCA 
TTTCCTTGTT TTGATGAGCC AATCTACAAG GCTACTTTCA AAATCAGCAT CAAGCATCAA 
GCAACCTATT TATCTTTATC TAATATGCCA GTGGAAACTT CCGTGTTTGA GGAAGATGGA 
TGGGTTACGG ATCACTTTTC ACAGACCCCT CTCATGTCCA CATATTATTT AGCCTGGGCA 
ATTTGCAACT TCACATACAG AGAAACTACC ACCAAGAGTG GGGTTGTAGT ACGATTATAT 
GCAAGACCTG ATGCTATCAG AAGAGGATCC GGGGACTATG CTCTCCATAT AACAAAGAGA 
TTAATAGAAT TTTATGAAGA CTACTTTAAA GTGCCCTATT CCTTGCCAAA ACTAGATCTT 
TTAGCTGTGC CTAAGCATCC GTATGCTGCT ATGGAGAACT GGGGACTAAG TATTTTTGTG 
GAACAAAGAA TACTGCTGGA TCCCAGTGTT TCATCTATTT CTTATTTGCT GGATGTCACC 
ATGGTCATTG TTCATGAGAT ATGTCACCAG TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG 
TGGGAAGACG TGTGGCTGAA GGAAGGGTTT GCTCACTACT TTGAATTTGT TGGTACAGAC 
TACCTCTATC CTGGCTGGAA CATGGAAAAG CAGAGGTTTC TGACCGATGT TCTGCATGAA 
GTGATGCTGC TGGACGGTTT GGCCAGTTCC CATCCAGTAT CACAGGAAGT GCTGCAGGCA 
ACAGATATTG ACAGGGTGTT TGACTGGATC GCATATAAAA AGGGTGCTGC TTTAATAAGA 
ATGCTGGCTA ATTTTATGGG CCATTCAGTT TTCCAGAGGG GTTTGCAAGA TTATTTAACC 
ATTCATAAGT ATGGCAATGC AGCCAGAAAT GATCTCTGGA ATACATTATC GGAGGCATTA 
AAAAGAAATG GGAAATATGT AAATATACAA GAAGTAATGG ATCAGTGGAC ACTCCAGATG 
GGTTATCCTG TTATCACCAT CTTGGGAAAC ACAACAGCAG AAAATAGAAT AATAATTACC 
CAACAGCATT TTATCTATGA TATCAGTGCT AAAACTAAAG CACTTAAACT TCAGAATAAC 
AGTTACCTGT GGCAGATTCC ATTAACTATT GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA 
GAAGCAATTA TTTGGGTGTC TAACAAATCA GAGCACCACA GAATAACTTA TTTGGACAAA 
GGAAGCTGGC TGCTGGGGAA CATCAATCAA ACTGGCTATT TTAGAGTCAA CTATGACCTA 
AGGAACTGGA GATTATTAAT TGATCAATTA ATCCGGAATC ATGAGGTTCT TTCTGTCAGT 
AACCGAGCGG GCTTGATCGA TGATGCCTTC AGCCTAGCCA GGGCTGGCTA TTTGCCTCAG 
AATATTCCTC TGGAGATTAT CAGATACCTG TCTGAGGAGA AGGATTTTCT TCCTTGGCAT 
GCTGCCAGCC GAGCTCTTTA TCCTCTAGAT AAATTACTGG ACCGCATGGA AAACTACAAC 
ATTTTCAATG AATATATCTT AAAGCAAGTT GCAACAACGT ATATCAAGCT TGGGTGGCCG 
AAAAATAATT TTAATGGATC TCTTGTTCAA GCATCCTACC AACATGAAGA ACTACGTAGA 
GAAGTTATAA TGCTGGCCTG CAGTTTTGGC AACAAGCACT GTCACCAACA GGCATCAACA 
CTTATTTCAG ATTGGATTTC CAGCAACAGG AACAGAATAC CACTAAATGT TAGAGACATC 
GTATACTGTA CAGGAGTGTC ACTGCTGGAT GAGGATGTCT GGGAATTCAT ATGGATGAAA 
TTCCATTCCA CCACAGCAGT TTCTGAGAAG AAAATATTAT TGGAAGCCTT AACTTGCAGT 
GATGACAGGA ATTTATTAAA CAGGCTTCTA AATCTGTCAC TGAATTCTGA GGTGGTGCTG 
GATCAAGATG CAATTGATGT CATAATCCAT GTAGCTCGAA ATCCACATGG TCGAGACCTT 
GCCTGGAAGT TTTTCAGGGA TAAATGGAAG ATATTAAATA CCAGTTGCAG TAAAATTTAC 
TCCCAGGACA TCTTGTTTCA TGGTGAGAAT TCTTTAGCCT CAATTTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TRHDE cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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