DDX50 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX50
Entrez Gene ID	450499
Full Name	DExD-box helicase 50
General protein information	Preferred Names DExD-box helicase 50 Names ATP-dependent RNA helicase DDX50 DEAD (Asp-Glu-Ala-Asp) box polypeptide 50 DEAD-box helicase 50 nucleolar protein GU2
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_507824.5	XP_507824.2	ATP-dependent RNA helicase DDX50 isoform X1
XM_016918457.1	XP_016773946.1	ATP-dependent RNA helicase DDX50 isoform X2
XM_009458593.2	XP_009456868.1	ATP-dependent RNA helicase DDX50 isoform X2
XM_016918457.2	XP_016773946.1	ATP-dependent RNA helicase DDX50 isoform X2
XM_009458591.2	XP_009456866.1	ATP-dependent RNA helicase DDX50 isoform X2
XM_009458591.3	XP_009456866.1	ATP-dependent RNA helicase DDX50 isoform X2
XM_009458593.3	XP_009456868.1	ATP-dependent RNA helicase DDX50 isoform X2
XM_507824.6	XP_507824.2	ATP-dependent RNA helicase DDX50 isoform X1

Pathways from BioSystems

Homologies

	DDX50	XP_001111043.1
Rattus norvegicus (Norway rat)	Ddx50	NP_001013216.2
Pan troglodytes (chimpanzee)	DDX50	XP_507824.2
Canis lupus familiaris (dog)	DDX50	XP_850174.2
Mus musculus (house mouse)	Ddx50	NP_444413.1
Arabidopsis thaliana (thale cress)	emb1138	NP_001031943.1
Oryza sativa (rice)	Os03g0827700	NP_001051773.1
Homo sapiens (human)	DDX50	NP_076950.1
Bos taurus (cattle)	DDX50	NP_001192982.1

Gene Ontology

The following DDX50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc29579	XM_009458591.3 Latest version!	Pan troglodytes DExD-box helicase 50 (DDX50), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
OPc29579	XM_016918457.2 Latest version!	Pan troglodytes DExD-box helicase 50 (DDX50), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
OPc29579	XM_009458593.3 Latest version!	Pan troglodytes DExD-box helicase 50 (DDX50), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
OPc13572	XM_507824.6 Latest version!	Pan troglodytes DExD-box helicase 50 (DDX50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OPc29579	XM_016918457.1	Pan troglodytes DEAD-box helicase 50 (DDX50), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
OPc29579	XM_009458591.2	Pan troglodytes DEAD-box helicase 50 (DDX50), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
OPc29579	XM_009458593.2	Pan troglodytes DEAD-box helicase 50 (DDX50), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
OPc13572	XM_507824.5	Pan troglodytes DEAD-box helicase 50 (DDX50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX50 ( XM_016918457.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016773946.1 Pan troglodytes DEAD-box helicase 50 (DDX50), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPc29579
Clone ID Related Accession (Same CDS sequence)	XM_016918457.1 , XM_009458593.2 , XM_016918457.2 , XM_009458591.2 , XM_009458591.3 , XM_009458593.3
Accession Version	XM_016918457.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	ATP-dependent RNA helicase DDX50 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006477.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGCTAAATGG AGACACTGAA GAAGGATTTA ATAGACTTTC AGATGAATTC 
TCCAAATCTC ATAAGTCAAG AAGAAAAGAT CTACCAAATG GAGATATAGA TGAATATGAA 
AAAAAATCAA AGCGAGTATC ATCCTTAGAT ACTTCTACTC ATAAATCAAG TGATAATAAA 
CTAGAGGAGA CCTTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT TCCTATTTCT 
GAAGAGACTA TAAAGCTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC TATTCAAGTT 
AAGACCTTTG GTCCTGTATA TGAAGGAAAA GATTTAATAG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATCCCCTTA ATTGAAAGAC TCCAAAGAAA TCAAGAAACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CAACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATATAACT AGGAAACTCA GCGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGA 
ACACCTGGTC GTATCAAAGA CCATCTGCAG AGTGGCCGAT TGGATCTTTC TAAACTGCGA 
CATGTTGTGC TTGATGAAGT GGATCAGATG TTAGATTTAG GTTTCGCTGA ACAAGTTGAA 
GATATTATTC ATGAATCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTACTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATACATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG AAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GTCATTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATTTTC TGTGAGACCA AGAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT TGCACAGTCA 
CAAAGAGAAA TTACACTAAA AGGCTTCAGA GAAGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAAGTTGACC TGGTGATTCA AAGTTCTCCT 
CCTCAGGATG TTGAGTCCTA TATCCATCGC TCTGGACGCA CAGGTAGAGC TGGACGGACA 
GGGATTTGTA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AACTAAGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTCTGGCT TCCGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAAGGTGCAG TGGATGCATT GGCTGCAGCT 
TTAGCCCACA TTTCTGGTGC ATCAAGCTTT GAACCACGAT CTTTGATCAC CTCTGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCTA GAGGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCAGTGTCTC AGATTACCAG AATGTGCCTC 
CTGAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCTACAA CTGAGTCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCCGA CTGGATACTC TCAGTGCCAG CCAAATTACC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGCTG GTCAAGTGGT 
CGATCAGGCC GGTCAGGCCG GTCAGGTGGT CGATCTGGCG GCCGGTCAGG TAGACAGAGT 
CGACAAGGAA GTCGCTCAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAACGGAGT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016773946.1
CDS	542..2560
Translation	MKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDIDEYEKKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRSFD

Target ORF information:

RefSeq Version	XM_016918457.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes DEAD-box helicase 50 (DDX50), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016918457.1

ORF Insert Sequence:

ATGAAAGAGA AGCTAAATGG AGACACTGAA GAAGGATTTA ATAGACTTTC AGATGAATTC 
TCCAAATCTC ATAAGTCAAG AAGAAAAGAT CTACCAAATG GAGATATAGA TGAATATGAA 
AAAAAATCAA AGCGAGTATC ATCCTTAGAT ACTTCTACTC ATAAATCAAG TGATAATAAA 
CTAGAGGAGA CCTTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT TCCTATTTCT 
GAAGAGACTA TAAAGCTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC TATTCAAGTT 
AAGACCTTTG GTCCTGTATA TGAAGGAAAA GATTTAATAG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATCCCCTTA ATTGAAAGAC TCCAAAGAAA TCAAGAAACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CAACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATATAACT AGGAAACTCA GCGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGA 
ACACCTGGTC GTATCAAAGA CCATCTGCAG AGTGGCCGAT TGGATCTTTC TAAACTGCGA 
CATGTTGTGC TTGATGAAGT GGATCAGATG TTAGATTTAG GTTTCGCTGA ACAAGTTGAA 
GATATTATTC ATGAATCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTACTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATACATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG AAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GTCATTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATTTTC TGTGAGACCA AGAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT TGCACAGTCA 
CAAAGAGAAA TTACACTAAA AGGCTTCAGA GAAGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAAGTTGACC TGGTGATTCA AAGTTCTCCT 
CCTCAGGATG TTGAGTCCTA TATCCATCGC TCTGGACGCA CAGGTAGAGC TGGACGGACA 
GGGATTTGTA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AACTAAGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTCTGGCT TCCGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAAGGTGCAG TGGATGCATT GGCTGCAGCT 
TTAGCCCACA TTTCTGGTGC ATCAAGCTTT GAACCACGAT CTTTGATCAC CTCTGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCTA GAGGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCAGTGTCTC AGATTACCAG AATGTGCCTC 
CTGAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCTACAA CTGAGTCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCCGA CTGGATACTC TCAGTGCCAG CCAAATTACC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGCTG GTCAAGTGGT 
CGATCAGGCC GGTCAGGCCG GTCAGGTGGT CGATCTGGCG GCCGGTCAGG TAGACAGAGT 
CGACAAGGAA GTCGCTCAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAACGGAGT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX50 ( XM_009458593.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009456868.1 Pan troglodytes DEAD-box helicase 50 (DDX50), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPc29579
Clone ID Related Accession (Same CDS sequence)	XM_016918457.1 , XM_009458593.2 , XM_016918457.2 , XM_009458591.2 , XM_009458591.3 , XM_009458593.3
Accession Version	XM_009458593.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	ATP-dependent RNA helicase DDX50 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006477.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009458593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGCTAAATGG AGACACTGAA GAAGGATTTA ATAGACTTTC AGATGAATTC 
TCCAAATCTC ATAAGTCAAG AAGAAAAGAT CTACCAAATG GAGATATAGA TGAATATGAA 
AAAAAATCAA AGCGAGTATC ATCCTTAGAT ACTTCTACTC ATAAATCAAG TGATAATAAA 
CTAGAGGAGA CCTTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT TCCTATTTCT 
GAAGAGACTA TAAAGCTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC TATTCAAGTT 
AAGACCTTTG GTCCTGTATA TGAAGGAAAA GATTTAATAG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATCCCCTTA ATTGAAAGAC TCCAAAGAAA TCAAGAAACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CAACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATATAACT AGGAAACTCA GCGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGA 
ACACCTGGTC GTATCAAAGA CCATCTGCAG AGTGGCCGAT TGGATCTTTC TAAACTGCGA 
CATGTTGTGC TTGATGAAGT GGATCAGATG TTAGATTTAG GTTTCGCTGA ACAAGTTGAA 
GATATTATTC ATGAATCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTACTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATACATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG AAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GTCATTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATTTTC TGTGAGACCA AGAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT TGCACAGTCA 
CAAAGAGAAA TTACACTAAA AGGCTTCAGA GAAGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAAGTTGACC TGGTGATTCA AAGTTCTCCT 
CCTCAGGATG TTGAGTCCTA TATCCATCGC TCTGGACGCA CAGGTAGAGC TGGACGGACA 
GGGATTTGTA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AACTAAGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTCTGGCT TCCGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAAGGTGCAG TGGATGCATT GGCTGCAGCT 
TTAGCCCACA TTTCTGGTGC ATCAAGCTTT GAACCACGAT CTTTGATCAC CTCTGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCTA GAGGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCAGTGTCTC AGATTACCAG AATGTGCCTC 
CTGAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCTACAA CTGAGTCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCCGA CTGGATACTC TCAGTGCCAG CCAAATTACC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGCTG GTCAAGTGGT 
CGATCAGGCC GGTCAGGCCG GTCAGGTGGT CGATCTGGCG GCCGGTCAGG TAGACAGAGT 
CGACAAGGAA GTCGCTCAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAACGGAGT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009456868.1
CDS	2089..4107
Translation	MKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDIDEYEKKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRSFD

Target ORF information:

RefSeq Version	XM_009458593.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes DEAD-box helicase 50 (DDX50), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009458593.2

ORF Insert Sequence:

ATGAAAGAGA AGCTAAATGG AGACACTGAA GAAGGATTTA ATAGACTTTC AGATGAATTC 
TCCAAATCTC ATAAGTCAAG AAGAAAAGAT CTACCAAATG GAGATATAGA TGAATATGAA 
AAAAAATCAA AGCGAGTATC ATCCTTAGAT ACTTCTACTC ATAAATCAAG TGATAATAAA 
CTAGAGGAGA CCTTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT TCCTATTTCT 
GAAGAGACTA TAAAGCTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC TATTCAAGTT 
AAGACCTTTG GTCCTGTATA TGAAGGAAAA GATTTAATAG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATCCCCTTA ATTGAAAGAC TCCAAAGAAA TCAAGAAACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CAACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATATAACT AGGAAACTCA GCGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGA 
ACACCTGGTC GTATCAAAGA CCATCTGCAG AGTGGCCGAT TGGATCTTTC TAAACTGCGA 
CATGTTGTGC TTGATGAAGT GGATCAGATG TTAGATTTAG GTTTCGCTGA ACAAGTTGAA 
GATATTATTC ATGAATCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTACTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATACATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG AAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GTCATTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATTTTC TGTGAGACCA AGAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT TGCACAGTCA 
CAAAGAGAAA TTACACTAAA AGGCTTCAGA GAAGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAAGTTGACC TGGTGATTCA AAGTTCTCCT 
CCTCAGGATG TTGAGTCCTA TATCCATCGC TCTGGACGCA CAGGTAGAGC TGGACGGACA 
GGGATTTGTA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AACTAAGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTCTGGCT TCCGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAAGGTGCAG TGGATGCATT GGCTGCAGCT 
TTAGCCCACA TTTCTGGTGC ATCAAGCTTT GAACCACGAT CTTTGATCAC CTCTGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCTA GAGGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCAGTGTCTC AGATTACCAG AATGTGCCTC 
CTGAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCTACAA CTGAGTCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCCGA CTGGATACTC TCAGTGCCAG CCAAATTACC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGCTG GTCAAGTGGT 
CGATCAGGCC GGTCAGGCCG GTCAGGTGGT CGATCTGGCG GCCGGTCAGG TAGACAGAGT 
CGACAAGGAA GTCGCTCAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAACGGAGT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX50 ( XM_016918457.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016773946.1 Pan troglodytes DExD-box helicase 50 (DDX50), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPc29579
Clone ID Related Accession (Same CDS sequence)	XM_016918457.1 , XM_009458593.2 , XM_016918457.2 , XM_009458591.2 , XM_009458591.3 , XM_009458593.3
Accession Version	XM_016918457.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	ATP-dependent RNA helicase DDX50 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036889.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016918457.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGCTAAATGG AGACACTGAA GAAGGATTTA ATAGACTTTC AGATGAATTC 
TCCAAATCTC ATAAGTCAAG AAGAAAAGAT CTACCAAATG GAGATATAGA TGAATATGAA 
AAAAAATCAA AGCGAGTATC ATCCTTAGAT ACTTCTACTC ATAAATCAAG TGATAATAAA 
CTAGAGGAGA CCTTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT TCCTATTTCT 
GAAGAGACTA TAAAGCTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC TATTCAAGTT 
AAGACCTTTG GTCCTGTATA TGAAGGAAAA GATTTAATAG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATCCCCTTA ATTGAAAGAC TCCAAAGAAA TCAAGAAACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CAACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATATAACT AGGAAACTCA GCGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGA 
ACACCTGGTC GTATCAAAGA CCATCTGCAG AGTGGCCGAT TGGATCTTTC TAAACTGCGA 
CATGTTGTGC TTGATGAAGT GGATCAGATG TTAGATTTAG GTTTCGCTGA ACAAGTTGAA 
GATATTATTC ATGAATCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTACTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATACATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG AAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GTCATTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATTTTC TGTGAGACCA AGAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT TGCACAGTCA 
CAAAGAGAAA TTACACTAAA AGGCTTCAGA GAAGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAAGTTGACC TGGTGATTCA AAGTTCTCCT 
CCTCAGGATG TTGAGTCCTA TATCCATCGC TCTGGACGCA CAGGTAGAGC TGGACGGACA 
GGGATTTGTA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AACTAAGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTCTGGCT TCCGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAAGGTGCAG TGGATGCATT GGCTGCAGCT 
TTAGCCCACA TTTCTGGTGC ATCAAGCTTT GAACCACGAT CTTTGATCAC CTCTGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCTA GAGGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCAGTGTCTC AGATTACCAG AATGTGCCTC 
CTGAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCTACAA CTGAGTCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCCGA CTGGATACTC TCAGTGCCAG CCAAATTACC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGCTG GTCAAGTGGT 
CGATCAGGCC GGTCAGGCCG GTCAGGTGGT CGATCTGGCG GCCGGTCAGG TAGACAGAGT 
CGACAAGGAA GTCGCTCAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAACGGAGT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016773946.1
CDS	547..2565
Translation	MKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDIDEYEKKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRSFD

Target ORF information:

RefSeq Version	XM_016918457.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes DExD-box helicase 50 (DDX50), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016918457.2

ORF Insert Sequence:

ATGAAAGAGA AGCTAAATGG AGACACTGAA GAAGGATTTA ATAGACTTTC AGATGAATTC 
TCCAAATCTC ATAAGTCAAG AAGAAAAGAT CTACCAAATG GAGATATAGA TGAATATGAA 
AAAAAATCAA AGCGAGTATC ATCCTTAGAT ACTTCTACTC ATAAATCAAG TGATAATAAA 
CTAGAGGAGA CCTTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT TCCTATTTCT 
GAAGAGACTA TAAAGCTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC TATTCAAGTT 
AAGACCTTTG GTCCTGTATA TGAAGGAAAA GATTTAATAG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATCCCCTTA ATTGAAAGAC TCCAAAGAAA TCAAGAAACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CAACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATATAACT AGGAAACTCA GCGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGA 
ACACCTGGTC GTATCAAAGA CCATCTGCAG AGTGGCCGAT TGGATCTTTC TAAACTGCGA 
CATGTTGTGC TTGATGAAGT GGATCAGATG TTAGATTTAG GTTTCGCTGA ACAAGTTGAA 
GATATTATTC ATGAATCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTACTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATACATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG AAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GTCATTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATTTTC TGTGAGACCA AGAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT TGCACAGTCA 
CAAAGAGAAA TTACACTAAA AGGCTTCAGA GAAGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAAGTTGACC TGGTGATTCA AAGTTCTCCT 
CCTCAGGATG TTGAGTCCTA TATCCATCGC TCTGGACGCA CAGGTAGAGC TGGACGGACA 
GGGATTTGTA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AACTAAGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTCTGGCT TCCGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAAGGTGCAG TGGATGCATT GGCTGCAGCT 
TTAGCCCACA TTTCTGGTGC ATCAAGCTTT GAACCACGAT CTTTGATCAC CTCTGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCTA GAGGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCAGTGTCTC AGATTACCAG AATGTGCCTC 
CTGAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCTACAA CTGAGTCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCCGA CTGGATACTC TCAGTGCCAG CCAAATTACC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGCTG GTCAAGTGGT 
CGATCAGGCC GGTCAGGCCG GTCAGGTGGT CGATCTGGCG GCCGGTCAGG TAGACAGAGT 
CGACAAGGAA GTCGCTCAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAACGGAGT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX50 ( XM_009458591.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009456866.1 Pan troglodytes DEAD-box helicase 50 (DDX50), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc29579
Clone ID Related Accession (Same CDS sequence)	XM_016918457.1 , XM_009458593.2 , XM_016918457.2 , XM_009458591.2 , XM_009458591.3 , XM_009458593.3
Accession Version	XM_009458591.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	ATP-dependent RNA helicase DDX50 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006477.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009458591.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGCTAAATGG AGACACTGAA GAAGGATTTA ATAGACTTTC AGATGAATTC 
TCCAAATCTC ATAAGTCAAG AAGAAAAGAT CTACCAAATG GAGATATAGA TGAATATGAA 
AAAAAATCAA AGCGAGTATC ATCCTTAGAT ACTTCTACTC ATAAATCAAG TGATAATAAA 
CTAGAGGAGA CCTTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT TCCTATTTCT 
GAAGAGACTA TAAAGCTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC TATTCAAGTT 
AAGACCTTTG GTCCTGTATA TGAAGGAAAA GATTTAATAG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATCCCCTTA ATTGAAAGAC TCCAAAGAAA TCAAGAAACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CAACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATATAACT AGGAAACTCA GCGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGA 
ACACCTGGTC GTATCAAAGA CCATCTGCAG AGTGGCCGAT TGGATCTTTC TAAACTGCGA 
CATGTTGTGC TTGATGAAGT GGATCAGATG TTAGATTTAG GTTTCGCTGA ACAAGTTGAA 
GATATTATTC ATGAATCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTACTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATACATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG AAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GTCATTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATTTTC TGTGAGACCA AGAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT TGCACAGTCA 
CAAAGAGAAA TTACACTAAA AGGCTTCAGA GAAGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAAGTTGACC TGGTGATTCA AAGTTCTCCT 
CCTCAGGATG TTGAGTCCTA TATCCATCGC TCTGGACGCA CAGGTAGAGC TGGACGGACA 
GGGATTTGTA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AACTAAGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTCTGGCT TCCGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAAGGTGCAG TGGATGCATT GGCTGCAGCT 
TTAGCCCACA TTTCTGGTGC ATCAAGCTTT GAACCACGAT CTTTGATCAC CTCTGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCTA GAGGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCAGTGTCTC AGATTACCAG AATGTGCCTC 
CTGAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCTACAA CTGAGTCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCCGA CTGGATACTC TCAGTGCCAG CCAAATTACC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGCTG GTCAAGTGGT 
CGATCAGGCC GGTCAGGCCG GTCAGGTGGT CGATCTGGCG GCCGGTCAGG TAGACAGAGT 
CGACAAGGAA GTCGCTCAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAACGGAGT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009456866.1
CDS	2147..4165
Translation	MKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDIDEYEKKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRSFD

Target ORF information:

RefSeq Version	XM_009458591.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes DEAD-box helicase 50 (DDX50), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009458591.2

ORF Insert Sequence:

ATGAAAGAGA AGCTAAATGG AGACACTGAA GAAGGATTTA ATAGACTTTC AGATGAATTC 
TCCAAATCTC ATAAGTCAAG AAGAAAAGAT CTACCAAATG GAGATATAGA TGAATATGAA 
AAAAAATCAA AGCGAGTATC ATCCTTAGAT ACTTCTACTC ATAAATCAAG TGATAATAAA 
CTAGAGGAGA CCTTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT TCCTATTTCT 
GAAGAGACTA TAAAGCTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC TATTCAAGTT 
AAGACCTTTG GTCCTGTATA TGAAGGAAAA GATTTAATAG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATCCCCTTA ATTGAAAGAC TCCAAAGAAA TCAAGAAACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CAACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATATAACT AGGAAACTCA GCGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGA 
ACACCTGGTC GTATCAAAGA CCATCTGCAG AGTGGCCGAT TGGATCTTTC TAAACTGCGA 
CATGTTGTGC TTGATGAAGT GGATCAGATG TTAGATTTAG GTTTCGCTGA ACAAGTTGAA 
GATATTATTC ATGAATCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTACTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATACATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG AAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GTCATTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATTTTC TGTGAGACCA AGAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT TGCACAGTCA 
CAAAGAGAAA TTACACTAAA AGGCTTCAGA GAAGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAAGTTGACC TGGTGATTCA AAGTTCTCCT 
CCTCAGGATG TTGAGTCCTA TATCCATCGC TCTGGACGCA CAGGTAGAGC TGGACGGACA 
GGGATTTGTA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AACTAAGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTCTGGCT TCCGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAAGGTGCAG TGGATGCATT GGCTGCAGCT 
TTAGCCCACA TTTCTGGTGC ATCAAGCTTT GAACCACGAT CTTTGATCAC CTCTGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCTA GAGGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCAGTGTCTC AGATTACCAG AATGTGCCTC 
CTGAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCTACAA CTGAGTCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCCGA CTGGATACTC TCAGTGCCAG CCAAATTACC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGCTG GTCAAGTGGT 
CGATCAGGCC GGTCAGGCCG GTCAGGTGGT CGATCTGGCG GCCGGTCAGG TAGACAGAGT 
CGACAAGGAA GTCGCTCAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAACGGAGT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX50 ( XM_009458591.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009456866.1 Pan troglodytes DExD-box helicase 50 (DDX50), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc29579
Clone ID Related Accession (Same CDS sequence)	XM_016918457.1 , XM_009458593.2 , XM_016918457.2 , XM_009458591.2 , XM_009458591.3 , XM_009458593.3
Accession Version	XM_009458591.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	ATP-dependent RNA helicase DDX50 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036889.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009458591.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGCTAAATGG AGACACTGAA GAAGGATTTA ATAGACTTTC AGATGAATTC 
TCCAAATCTC ATAAGTCAAG AAGAAAAGAT CTACCAAATG GAGATATAGA TGAATATGAA 
AAAAAATCAA AGCGAGTATC ATCCTTAGAT ACTTCTACTC ATAAATCAAG TGATAATAAA 
CTAGAGGAGA CCTTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT TCCTATTTCT 
GAAGAGACTA TAAAGCTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC TATTCAAGTT 
AAGACCTTTG GTCCTGTATA TGAAGGAAAA GATTTAATAG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATCCCCTTA ATTGAAAGAC TCCAAAGAAA TCAAGAAACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CAACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATATAACT AGGAAACTCA GCGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGA 
ACACCTGGTC GTATCAAAGA CCATCTGCAG AGTGGCCGAT TGGATCTTTC TAAACTGCGA 
CATGTTGTGC TTGATGAAGT GGATCAGATG TTAGATTTAG GTTTCGCTGA ACAAGTTGAA 
GATATTATTC ATGAATCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTACTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATACATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG AAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GTCATTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATTTTC TGTGAGACCA AGAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT TGCACAGTCA 
CAAAGAGAAA TTACACTAAA AGGCTTCAGA GAAGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAAGTTGACC TGGTGATTCA AAGTTCTCCT 
CCTCAGGATG TTGAGTCCTA TATCCATCGC TCTGGACGCA CAGGTAGAGC TGGACGGACA 
GGGATTTGTA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AACTAAGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTCTGGCT TCCGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAAGGTGCAG TGGATGCATT GGCTGCAGCT 
TTAGCCCACA TTTCTGGTGC ATCAAGCTTT GAACCACGAT CTTTGATCAC CTCTGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCTA GAGGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCAGTGTCTC AGATTACCAG AATGTGCCTC 
CTGAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCTACAA CTGAGTCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCCGA CTGGATACTC TCAGTGCCAG CCAAATTACC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGCTG GTCAAGTGGT 
CGATCAGGCC GGTCAGGCCG GTCAGGTGGT CGATCTGGCG GCCGGTCAGG TAGACAGAGT 
CGACAAGGAA GTCGCTCAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAACGGAGT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009456866.1
CDS	2145..4163
Translation	MKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDIDEYEKKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRSFD

Target ORF information:

RefSeq Version	XM_009458591.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes DExD-box helicase 50 (DDX50), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009458591.3

ORF Insert Sequence:

ATGAAAGAGA AGCTAAATGG AGACACTGAA GAAGGATTTA ATAGACTTTC AGATGAATTC 
TCCAAATCTC ATAAGTCAAG AAGAAAAGAT CTACCAAATG GAGATATAGA TGAATATGAA 
AAAAAATCAA AGCGAGTATC ATCCTTAGAT ACTTCTACTC ATAAATCAAG TGATAATAAA 
CTAGAGGAGA CCTTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT TCCTATTTCT 
GAAGAGACTA TAAAGCTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC TATTCAAGTT 
AAGACCTTTG GTCCTGTATA TGAAGGAAAA GATTTAATAG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATCCCCTTA ATTGAAAGAC TCCAAAGAAA TCAAGAAACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CAACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATATAACT AGGAAACTCA GCGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGA 
ACACCTGGTC GTATCAAAGA CCATCTGCAG AGTGGCCGAT TGGATCTTTC TAAACTGCGA 
CATGTTGTGC TTGATGAAGT GGATCAGATG TTAGATTTAG GTTTCGCTGA ACAAGTTGAA 
GATATTATTC ATGAATCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTACTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATACATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG AAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GTCATTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATTTTC TGTGAGACCA AGAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT TGCACAGTCA 
CAAAGAGAAA TTACACTAAA AGGCTTCAGA GAAGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAAGTTGACC TGGTGATTCA AAGTTCTCCT 
CCTCAGGATG TTGAGTCCTA TATCCATCGC TCTGGACGCA CAGGTAGAGC TGGACGGACA 
GGGATTTGTA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AACTAAGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTCTGGCT TCCGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAAGGTGCAG TGGATGCATT GGCTGCAGCT 
TTAGCCCACA TTTCTGGTGC ATCAAGCTTT GAACCACGAT CTTTGATCAC CTCTGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCTA GAGGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCAGTGTCTC AGATTACCAG AATGTGCCTC 
CTGAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCTACAA CTGAGTCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCCGA CTGGATACTC TCAGTGCCAG CCAAATTACC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGCTG GTCAAGTGGT 
CGATCAGGCC GGTCAGGCCG GTCAGGTGGT CGATCTGGCG GCCGGTCAGG TAGACAGAGT 
CGACAAGGAA GTCGCTCAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAACGGAGT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX50 ( XM_009458593.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009456868.1 Pan troglodytes DExD-box helicase 50 (DDX50), transcript variant X3, mRNA.

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CloneID	OPc29579
Clone ID Related Accession (Same CDS sequence)	XM_016918457.1 , XM_009458593.2 , XM_016918457.2 , XM_009458591.2 , XM_009458591.3 , XM_009458593.3
Accession Version	XM_009458593.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	ATP-dependent RNA helicase DDX50 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036889.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009458593.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGCTAAATGG AGACACTGAA GAAGGATTTA ATAGACTTTC AGATGAATTC 
TCCAAATCTC ATAAGTCAAG AAGAAAAGAT CTACCAAATG GAGATATAGA TGAATATGAA 
AAAAAATCAA AGCGAGTATC ATCCTTAGAT ACTTCTACTC ATAAATCAAG TGATAATAAA 
CTAGAGGAGA CCTTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT TCCTATTTCT 
GAAGAGACTA TAAAGCTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC TATTCAAGTT 
AAGACCTTTG GTCCTGTATA TGAAGGAAAA GATTTAATAG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATCCCCTTA ATTGAAAGAC TCCAAAGAAA TCAAGAAACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CAACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATATAACT AGGAAACTCA GCGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGA 
ACACCTGGTC GTATCAAAGA CCATCTGCAG AGTGGCCGAT TGGATCTTTC TAAACTGCGA 
CATGTTGTGC TTGATGAAGT GGATCAGATG TTAGATTTAG GTTTCGCTGA ACAAGTTGAA 
GATATTATTC ATGAATCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTACTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATACATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG AAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GTCATTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATTTTC TGTGAGACCA AGAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT TGCACAGTCA 
CAAAGAGAAA TTACACTAAA AGGCTTCAGA GAAGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAAGTTGACC TGGTGATTCA AAGTTCTCCT 
CCTCAGGATG TTGAGTCCTA TATCCATCGC TCTGGACGCA CAGGTAGAGC TGGACGGACA 
GGGATTTGTA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AACTAAGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTCTGGCT TCCGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAAGGTGCAG TGGATGCATT GGCTGCAGCT 
TTAGCCCACA TTTCTGGTGC ATCAAGCTTT GAACCACGAT CTTTGATCAC CTCTGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCTA GAGGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCAGTGTCTC AGATTACCAG AATGTGCCTC 
CTGAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCTACAA CTGAGTCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCCGA CTGGATACTC TCAGTGCCAG CCAAATTACC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGCTG GTCAAGTGGT 
CGATCAGGCC GGTCAGGCCG GTCAGGTGGT CGATCTGGCG GCCGGTCAGG TAGACAGAGT 
CGACAAGGAA GTCGCTCAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAACGGAGT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009456868.1
CDS	2087..4105
Translation	MKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDIDEYEKKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRSFD

Target ORF information:

RefSeq Version	XM_009458593.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes DExD-box helicase 50 (DDX50), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009458593.3

ORF Insert Sequence:

ATGAAAGAGA AGCTAAATGG AGACACTGAA GAAGGATTTA ATAGACTTTC AGATGAATTC 
TCCAAATCTC ATAAGTCAAG AAGAAAAGAT CTACCAAATG GAGATATAGA TGAATATGAA 
AAAAAATCAA AGCGAGTATC ATCCTTAGAT ACTTCTACTC ATAAATCAAG TGATAATAAA 
CTAGAGGAGA CCTTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT TCCTATTTCT 
GAAGAGACTA TAAAGCTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC TATTCAAGTT 
AAGACCTTTG GTCCTGTATA TGAAGGAAAA GATTTAATAG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATCCCCTTA ATTGAAAGAC TCCAAAGAAA TCAAGAAACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CAACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATATAACT AGGAAACTCA GCGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGA 
ACACCTGGTC GTATCAAAGA CCATCTGCAG AGTGGCCGAT TGGATCTTTC TAAACTGCGA 
CATGTTGTGC TTGATGAAGT GGATCAGATG TTAGATTTAG GTTTCGCTGA ACAAGTTGAA 
GATATTATTC ATGAATCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTACTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATACATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG AAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GTCATTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATTTTC TGTGAGACCA AGAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT TGCACAGTCA 
CAAAGAGAAA TTACACTAAA AGGCTTCAGA GAAGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAAGTTGACC TGGTGATTCA AAGTTCTCCT 
CCTCAGGATG TTGAGTCCTA TATCCATCGC TCTGGACGCA CAGGTAGAGC TGGACGGACA 
GGGATTTGTA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AACTAAGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTCTGGCT TCCGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAAGGTGCAG TGGATGCATT GGCTGCAGCT 
TTAGCCCACA TTTCTGGTGC ATCAAGCTTT GAACCACGAT CTTTGATCAC CTCTGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCTA GAGGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCAGTGTCTC AGATTACCAG AATGTGCCTC 
CTGAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCTACAA CTGAGTCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCCGA CTGGATACTC TCAGTGCCAG CCAAATTACC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGCTG GTCAAGTGGT 
CGATCAGGCC GGTCAGGCCG GTCAGGTGGT CGATCTGGCG GCCGGTCAGG TAGACAGAGT 
CGACAAGGAA GTCGCTCAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAACGGAGT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX50 ( XM_507824.5 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_507824.2 Pan troglodytes DEAD-box helicase 50 (DDX50), transcript variant X1, mRNA.

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CloneID	OPc13572
Clone ID Related Accession (Same CDS sequence)	XM_507824.5 , XM_507824.6
Accession Version	XM_507824.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	ATP-dependent RNA helicase DDX50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006477.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_507824.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGGA AACTCCTCTG GGGGGACATT ATGGAGCTGG AAGCACCCTT GGAGGAGTCC 
GAGAGCCAGA AGAAGGAGAG GCAAAAGAGT GACAGAAGGA AGTCAAGGCA CCATTATGAC 
TCGGATGAGA AATCAGAAAC AAGAGAAAAT GGTATTACAG ATGACCTGGA TGCTCCCAAG 
GCCAAAAAAT CTAAAATGAA AGAGAAGCTA AATGGAGACA CTGAAGAAGG ATTTAATAGA 
CTTTCAGATG AATTCTCCAA ATCTCATAAG TCAAGAAGAA AAGATCTACC AAATGGAGAT 
ATAGATGAAT ATGAAAAAAA ATCAAAGCGA GTATCATCCT TAGATACTTC TACTCATAAA 
TCAAGTGATA ATAAACTAGA GGAGACCTTA ACACGTGAAC AGAAAGAAGG AGCCTTCTCC 
AATTTTCCTA TTTCTGAAGA GACTATAAAG CTTCTGAAAG GTCGAGGGGT AACATATCTC 
TTTCCTATTC AAGTTAAGAC CTTTGGTCCT GTATATGAAG GAAAAGATTT AATAGCTCAA 
GCACGGACAG GAACAGGAAA GACATTCTCT TTTGCCATCC CCTTAATTGA AAGACTCCAA 
AGAAATCAAG AAACAATTAA AAAAAGCCGC TCACCAAAGG TACTTGTTTT GGCTCCAACA 
AGGGAACTGG CAAACCAAGT AGCCAAAGAC TTCAAAGATA TAACTAGGAA ACTCAGCGTG 
GCGTGTTTTT ATGGTGGAAC ATCATATCAA AGCCAAATTA ATCATATTCG AAATGGTATT 
GACATCTTGG TTGGAACACC TGGTCGTATC AAAGACCATC TGCAGAGTGG CCGATTGGAT 
CTTTCTAAAC TGCGACATGT TGTGCTTGAT GAAGTGGATC AGATGTTAGA TTTAGGTTTC 
GCTGAACAAG TTGAAGATAT TATTCATGAA TCCTACAAAA CTGATTCTGA AGACAATCCT 
CAGACTTTAC TTTTTTCTGC AACTTGCCCA CAGTGGGTAT ACAAAGTTGC AAAAAAATAC 
ATGAAATCCA GATATGAACA GGTTGACCTT GTTGGAAAAA TGACTCAAAA GGCTGCAACT 
ACTGTGGAAC ATTTGGCCAT CCAGTGTCAT TGGTCTCAGA GGCCAGCAGT TATTGGAGAT 
GTCCTTCAAG TCTACAGTGG GTCTGAAGGG AGGGCTATTA TTTTCTGTGA GACCAAGAAG 
AATGTAACTG AAATGGCCAT GAATCCACAC ATAAAACAGA ATGCCCAGTG TTTACATGGG 
GACATTGCAC AGTCACAAAG AGAAATTACA CTAAAAGGCT TCAGAGAAGG TAGTTTTAAA 
GTTTTGGTGG CAACCAATGT GGCTGCCCGT GGTTTGGACA TTCCTGAAGT TGACCTGGTG 
ATTCAAAGTT CTCCTCCTCA GGATGTTGAG TCCTATATCC ATCGCTCTGG ACGCACAGGT 
AGAGCTGGAC GGACAGGGAT TTGTATATGT TTTTATCAAC CAAGAGAAAG AGGTCAACTA 
AGATATGTGG AACAAAAAGC AGGAATTACT TTTAAACGTG TAGGTGTTCC TTCTACAATG 
GATTTAGTTA AATCTAAAAG CATGGATGCC ATCAGGTCTC TGGCTTCCGT TTCTTATGCT 
GCTGTTGATT TTTTCCGACC ATCAGCTCAG AGACTGATAG AAGAGAAAGG TGCAGTGGAT 
GCATTGGCTG CAGCTTTAGC CCACATTTCT GGTGCATCAA GCTTTGAACC ACGATCTTTG 
ATCACCTCTG ATAAGGGGTT TGTGACCATG ACTCTGGAAA GCCTAGAGGA AATACAGGAT 
GTCAGCTGTG CTTGGAAAGA ACTTAACAGA AAGCTGAGTA GTAATGCAGT GTCTCAGATT 
ACCAGAATGT GCCTCCTGAA AGGAAATATG GGTGTTTGCT TTGATGTTCC TACAACTGAG 
TCAGAAAGGT TACAGGCAGA GTGGCATGAT TCCGACTGGA TACTCTCAGT GCCAGCCAAA 
TTACCTGAAA TTGAAGAATA TTATGATGGA AACACATCTT CTAATTCCAG ACAGAGGAGT 
GGCTGGTCAA GTGGTCGATC AGGCCGGTCA GGCCGGTCAG GTGGTCGATC TGGCGGCCGG 
TCAGGTAGAC AGAGTCGACA AGGAAGTCGC TCAGGAAGTC GACAAGATGG TAGAAGACGA 
AGTGGGAATA GAAATCGATC AAGAAGTGGG GGCCACAAAC GGAGTTTTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_507824.2
CDS	1840..4053
Translation	MPGKLLWGDIMELEAPLEESESQKKERQKSDRRKSRHHYDSDEKSETRENGITDDLDAPKAKKSKMKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDIDEYEKKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRSFD

Target ORF information:

RefSeq Version	XM_507824.5
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes DEAD-box helicase 50 (DDX50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_507824.5

ORF Insert Sequence:

ATGCCTGGGA AACTCCTCTG GGGGGACATT ATGGAGCTGG AAGCACCCTT GGAGGAGTCC 
GAGAGCCAGA AGAAGGAGAG GCAAAAGAGT GACAGAAGGA AGTCAAGGCA CCATTATGAC 
TCGGATGAGA AATCAGAAAC AAGAGAAAAT GGTATTACAG ATGACCTGGA TGCTCCCAAG 
GCCAAAAAAT CTAAAATGAA AGAGAAGCTA AATGGAGACA CTGAAGAAGG ATTTAATAGA 
CTTTCAGATG AATTCTCCAA ATCTCATAAG TCAAGAAGAA AAGATCTACC AAATGGAGAT 
ATAGATGAAT ATGAAAAAAA ATCAAAGCGA GTATCATCCT TAGATACTTC TACTCATAAA 
TCAAGTGATA ATAAACTAGA GGAGACCTTA ACACGTGAAC AGAAAGAAGG AGCCTTCTCC 
AATTTTCCTA TTTCTGAAGA GACTATAAAG CTTCTGAAAG GTCGAGGGGT AACATATCTC 
TTTCCTATTC AAGTTAAGAC CTTTGGTCCT GTATATGAAG GAAAAGATTT AATAGCTCAA 
GCACGGACAG GAACAGGAAA GACATTCTCT TTTGCCATCC CCTTAATTGA AAGACTCCAA 
AGAAATCAAG AAACAATTAA AAAAAGCCGC TCACCAAAGG TACTTGTTTT GGCTCCAACA 
AGGGAACTGG CAAACCAAGT AGCCAAAGAC TTCAAAGATA TAACTAGGAA ACTCAGCGTG 
GCGTGTTTTT ATGGTGGAAC ATCATATCAA AGCCAAATTA ATCATATTCG AAATGGTATT 
GACATCTTGG TTGGAACACC TGGTCGTATC AAAGACCATC TGCAGAGTGG CCGATTGGAT 
CTTTCTAAAC TGCGACATGT TGTGCTTGAT GAAGTGGATC AGATGTTAGA TTTAGGTTTC 
GCTGAACAAG TTGAAGATAT TATTCATGAA TCCTACAAAA CTGATTCTGA AGACAATCCT 
CAGACTTTAC TTTTTTCTGC AACTTGCCCA CAGTGGGTAT ACAAAGTTGC AAAAAAATAC 
ATGAAATCCA GATATGAACA GGTTGACCTT GTTGGAAAAA TGACTCAAAA GGCTGCAACT 
ACTGTGGAAC ATTTGGCCAT CCAGTGTCAT TGGTCTCAGA GGCCAGCAGT TATTGGAGAT 
GTCCTTCAAG TCTACAGTGG GTCTGAAGGG AGGGCTATTA TTTTCTGTGA GACCAAGAAG 
AATGTAACTG AAATGGCCAT GAATCCACAC ATAAAACAGA ATGCCCAGTG TTTACATGGG 
GACATTGCAC AGTCACAAAG AGAAATTACA CTAAAAGGCT TCAGAGAAGG TAGTTTTAAA 
GTTTTGGTGG CAACCAATGT GGCTGCCCGT GGTTTGGACA TTCCTGAAGT TGACCTGGTG 
ATTCAAAGTT CTCCTCCTCA GGATGTTGAG TCCTATATCC ATCGCTCTGG ACGCACAGGT 
AGAGCTGGAC GGACAGGGAT TTGTATATGT TTTTATCAAC CAAGAGAAAG AGGTCAACTA 
AGATATGTGG AACAAAAAGC AGGAATTACT TTTAAACGTG TAGGTGTTCC TTCTACAATG 
GATTTAGTTA AATCTAAAAG CATGGATGCC ATCAGGTCTC TGGCTTCCGT TTCTTATGCT 
GCTGTTGATT TTTTCCGACC ATCAGCTCAG AGACTGATAG AAGAGAAAGG TGCAGTGGAT 
GCATTGGCTG CAGCTTTAGC CCACATTTCT GGTGCATCAA GCTTTGAACC ACGATCTTTG 
ATCACCTCTG ATAAGGGGTT TGTGACCATG ACTCTGGAAA GCCTAGAGGA AATACAGGAT 
GTCAGCTGTG CTTGGAAAGA ACTTAACAGA AAGCTGAGTA GTAATGCAGT GTCTCAGATT 
ACCAGAATGT GCCTCCTGAA AGGAAATATG GGTGTTTGCT TTGATGTTCC TACAACTGAG 
TCAGAAAGGT TACAGGCAGA GTGGCATGAT TCCGACTGGA TACTCTCAGT GCCAGCCAAA 
TTACCTGAAA TTGAAGAATA TTATGATGGA AACACATCTT CTAATTCCAG ACAGAGGAGT 
GGCTGGTCAA GTGGTCGATC AGGCCGGTCA GGCCGGTCAG GTGGTCGATC TGGCGGCCGG 
TCAGGTAGAC AGAGTCGACA AGGAAGTCGC TCAGGAAGTC GACAAGATGG TAGAAGACGA 
AGTGGGAATA GAAATCGATC AAGAAGTGGG GGCCACAAAC GGAGTTTTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX50 ( XM_507824.6 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_507824.2 Pan troglodytes DExD-box helicase 50 (DDX50), transcript variant X1, mRNA.

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CloneID	OPc13572
Clone ID Related Accession (Same CDS sequence)	XM_507824.5 , XM_507824.6
Accession Version	XM_507824.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	ATP-dependent RNA helicase DDX50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036889.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_507824.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGGA AACTCCTCTG GGGGGACATT ATGGAGCTGG AAGCACCCTT GGAGGAGTCC 
GAGAGCCAGA AGAAGGAGAG GCAAAAGAGT GACAGAAGGA AGTCAAGGCA CCATTATGAC 
TCGGATGAGA AATCAGAAAC AAGAGAAAAT GGTATTACAG ATGACCTGGA TGCTCCCAAG 
GCCAAAAAAT CTAAAATGAA AGAGAAGCTA AATGGAGACA CTGAAGAAGG ATTTAATAGA 
CTTTCAGATG AATTCTCCAA ATCTCATAAG TCAAGAAGAA AAGATCTACC AAATGGAGAT 
ATAGATGAAT ATGAAAAAAA ATCAAAGCGA GTATCATCCT TAGATACTTC TACTCATAAA 
TCAAGTGATA ATAAACTAGA GGAGACCTTA ACACGTGAAC AGAAAGAAGG AGCCTTCTCC 
AATTTTCCTA TTTCTGAAGA GACTATAAAG CTTCTGAAAG GTCGAGGGGT AACATATCTC 
TTTCCTATTC AAGTTAAGAC CTTTGGTCCT GTATATGAAG GAAAAGATTT AATAGCTCAA 
GCACGGACAG GAACAGGAAA GACATTCTCT TTTGCCATCC CCTTAATTGA AAGACTCCAA 
AGAAATCAAG AAACAATTAA AAAAAGCCGC TCACCAAAGG TACTTGTTTT GGCTCCAACA 
AGGGAACTGG CAAACCAAGT AGCCAAAGAC TTCAAAGATA TAACTAGGAA ACTCAGCGTG 
GCGTGTTTTT ATGGTGGAAC ATCATATCAA AGCCAAATTA ATCATATTCG AAATGGTATT 
GACATCTTGG TTGGAACACC TGGTCGTATC AAAGACCATC TGCAGAGTGG CCGATTGGAT 
CTTTCTAAAC TGCGACATGT TGTGCTTGAT GAAGTGGATC AGATGTTAGA TTTAGGTTTC 
GCTGAACAAG TTGAAGATAT TATTCATGAA TCCTACAAAA CTGATTCTGA AGACAATCCT 
CAGACTTTAC TTTTTTCTGC AACTTGCCCA CAGTGGGTAT ACAAAGTTGC AAAAAAATAC 
ATGAAATCCA GATATGAACA GGTTGACCTT GTTGGAAAAA TGACTCAAAA GGCTGCAACT 
ACTGTGGAAC ATTTGGCCAT CCAGTGTCAT TGGTCTCAGA GGCCAGCAGT TATTGGAGAT 
GTCCTTCAAG TCTACAGTGG GTCTGAAGGG AGGGCTATTA TTTTCTGTGA GACCAAGAAG 
AATGTAACTG AAATGGCCAT GAATCCACAC ATAAAACAGA ATGCCCAGTG TTTACATGGG 
GACATTGCAC AGTCACAAAG AGAAATTACA CTAAAAGGCT TCAGAGAAGG TAGTTTTAAA 
GTTTTGGTGG CAACCAATGT GGCTGCCCGT GGTTTGGACA TTCCTGAAGT TGACCTGGTG 
ATTCAAAGTT CTCCTCCTCA GGATGTTGAG TCCTATATCC ATCGCTCTGG ACGCACAGGT 
AGAGCTGGAC GGACAGGGAT TTGTATATGT TTTTATCAAC CAAGAGAAAG AGGTCAACTA 
AGATATGTGG AACAAAAAGC AGGAATTACT TTTAAACGTG TAGGTGTTCC TTCTACAATG 
GATTTAGTTA AATCTAAAAG CATGGATGCC ATCAGGTCTC TGGCTTCCGT TTCTTATGCT 
GCTGTTGATT TTTTCCGACC ATCAGCTCAG AGACTGATAG AAGAGAAAGG TGCAGTGGAT 
GCATTGGCTG CAGCTTTAGC CCACATTTCT GGTGCATCAA GCTTTGAACC ACGATCTTTG 
ATCACCTCTG ATAAGGGGTT TGTGACCATG ACTCTGGAAA GCCTAGAGGA AATACAGGAT 
GTCAGCTGTG CTTGGAAAGA ACTTAACAGA AAGCTGAGTA GTAATGCAGT GTCTCAGATT 
ACCAGAATGT GCCTCCTGAA AGGAAATATG GGTGTTTGCT TTGATGTTCC TACAACTGAG 
TCAGAAAGGT TACAGGCAGA GTGGCATGAT TCCGACTGGA TACTCTCAGT GCCAGCCAAA 
TTACCTGAAA TTGAAGAATA TTATGATGGA AACACATCTT CTAATTCCAG ACAGAGGAGT 
GGCTGGTCAA GTGGTCGATC AGGCCGGTCA GGCCGGTCAG GTGGTCGATC TGGCGGCCGG 
TCAGGTAGAC AGAGTCGACA AGGAAGTCGC TCAGGAAGTC GACAAGATGG TAGAAGACGA 
AGTGGGAATA GAAATCGATC AAGAAGTGGG GGCCACAAAC GGAGTTTTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_507824.2
CDS	1838..4051
Translation	MPGKLLWGDIMELEAPLEESESQKKERQKSDRRKSRHHYDSDEKSETRENGITDDLDAPKAKKSKMKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDIDEYEKKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRSFD

Target ORF information:

RefSeq Version	XM_507824.6
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes DExD-box helicase 50 (DDX50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_507824.6

ORF Insert Sequence:

ATGCCTGGGA AACTCCTCTG GGGGGACATT ATGGAGCTGG AAGCACCCTT GGAGGAGTCC 
GAGAGCCAGA AGAAGGAGAG GCAAAAGAGT GACAGAAGGA AGTCAAGGCA CCATTATGAC 
TCGGATGAGA AATCAGAAAC AAGAGAAAAT GGTATTACAG ATGACCTGGA TGCTCCCAAG 
GCCAAAAAAT CTAAAATGAA AGAGAAGCTA AATGGAGACA CTGAAGAAGG ATTTAATAGA 
CTTTCAGATG AATTCTCCAA ATCTCATAAG TCAAGAAGAA AAGATCTACC AAATGGAGAT 
ATAGATGAAT ATGAAAAAAA ATCAAAGCGA GTATCATCCT TAGATACTTC TACTCATAAA 
TCAAGTGATA ATAAACTAGA GGAGACCTTA ACACGTGAAC AGAAAGAAGG AGCCTTCTCC 
AATTTTCCTA TTTCTGAAGA GACTATAAAG CTTCTGAAAG GTCGAGGGGT AACATATCTC 
TTTCCTATTC AAGTTAAGAC CTTTGGTCCT GTATATGAAG GAAAAGATTT AATAGCTCAA 
GCACGGACAG GAACAGGAAA GACATTCTCT TTTGCCATCC CCTTAATTGA AAGACTCCAA 
AGAAATCAAG AAACAATTAA AAAAAGCCGC TCACCAAAGG TACTTGTTTT GGCTCCAACA 
AGGGAACTGG CAAACCAAGT AGCCAAAGAC TTCAAAGATA TAACTAGGAA ACTCAGCGTG 
GCGTGTTTTT ATGGTGGAAC ATCATATCAA AGCCAAATTA ATCATATTCG AAATGGTATT 
GACATCTTGG TTGGAACACC TGGTCGTATC AAAGACCATC TGCAGAGTGG CCGATTGGAT 
CTTTCTAAAC TGCGACATGT TGTGCTTGAT GAAGTGGATC AGATGTTAGA TTTAGGTTTC 
GCTGAACAAG TTGAAGATAT TATTCATGAA TCCTACAAAA CTGATTCTGA AGACAATCCT 
CAGACTTTAC TTTTTTCTGC AACTTGCCCA CAGTGGGTAT ACAAAGTTGC AAAAAAATAC 
ATGAAATCCA GATATGAACA GGTTGACCTT GTTGGAAAAA TGACTCAAAA GGCTGCAACT 
ACTGTGGAAC ATTTGGCCAT CCAGTGTCAT TGGTCTCAGA GGCCAGCAGT TATTGGAGAT 
GTCCTTCAAG TCTACAGTGG GTCTGAAGGG AGGGCTATTA TTTTCTGTGA GACCAAGAAG 
AATGTAACTG AAATGGCCAT GAATCCACAC ATAAAACAGA ATGCCCAGTG TTTACATGGG 
GACATTGCAC AGTCACAAAG AGAAATTACA CTAAAAGGCT TCAGAGAAGG TAGTTTTAAA 
GTTTTGGTGG CAACCAATGT GGCTGCCCGT GGTTTGGACA TTCCTGAAGT TGACCTGGTG 
ATTCAAAGTT CTCCTCCTCA GGATGTTGAG TCCTATATCC ATCGCTCTGG ACGCACAGGT 
AGAGCTGGAC GGACAGGGAT TTGTATATGT TTTTATCAAC CAAGAGAAAG AGGTCAACTA 
AGATATGTGG AACAAAAAGC AGGAATTACT TTTAAACGTG TAGGTGTTCC TTCTACAATG 
GATTTAGTTA AATCTAAAAG CATGGATGCC ATCAGGTCTC TGGCTTCCGT TTCTTATGCT 
GCTGTTGATT TTTTCCGACC ATCAGCTCAG AGACTGATAG AAGAGAAAGG TGCAGTGGAT 
GCATTGGCTG CAGCTTTAGC CCACATTTCT GGTGCATCAA GCTTTGAACC ACGATCTTTG 
ATCACCTCTG ATAAGGGGTT TGTGACCATG ACTCTGGAAA GCCTAGAGGA AATACAGGAT 
GTCAGCTGTG CTTGGAAAGA ACTTAACAGA AAGCTGAGTA GTAATGCAGT GTCTCAGATT 
ACCAGAATGT GCCTCCTGAA AGGAAATATG GGTGTTTGCT TTGATGTTCC TACAACTGAG 
TCAGAAAGGT TACAGGCAGA GTGGCATGAT TCCGACTGGA TACTCTCAGT GCCAGCCAAA 
TTACCTGAAA TTGAAGAATA TTATGATGGA AACACATCTT CTAATTCCAG ACAGAGGAGT 
GGCTGGTCAA GTGGTCGATC AGGCCGGTCA GGCCGGTCAG GTGGTCGATC TGGCGGCCGG 
TCAGGTAGAC AGAGTCGACA AGGAAGTCGC TCAGGAAGTC GACAAGATGG TAGAAGACGA 
AGTGGGAATA GAAATCGATC AAGAAGTGGG GGCCACAAAC GGAGTTTTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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DDX50 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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