DDX50 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX50
Entrez Gene ID	534331
Full Name	DExD-box helicase 50
General protein information	Preferred Names DExD-box helicase 50 Names ATP-dependent RNA helicase DDX50 DEAD (Asp-Glu-Ala-Asp) box polypeptide 50 DEAD-box helicase 50
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001206053.1	NP_001192982.1	ATP-dependent RNA helicase DDX50
XM_015461003.1	XP_015316489.1	ATP-dependent RNA helicase DDX50 isoform X1
XM_024986700.1	XP_024842468.1	ATP-dependent RNA helicase DDX50 isoform X1
XM_005226430.3	XP_005226487.1	ATP-dependent RNA helicase DDX50 isoform X1
XM_010820595.2	XP_010818897.1	ATP-dependent RNA helicase DDX50 isoform X1
XM_010820595.3	XP_010818897.1	ATP-dependent RNA helicase DDX50 isoform X1
NM_001206053.2	NP_001192982.1	ATP-dependent RNA helicase DDX50
XM_005226430.4	XP_005226487.1	ATP-dependent RNA helicase DDX50 isoform X1

Pathways from BioSystems

Homologies

	DDX50	XP_001111043.1
Rattus norvegicus (Norway rat)	Ddx50	NP_001013216.2
Pan troglodytes (chimpanzee)	DDX50	XP_507824.2
Canis lupus familiaris (dog)	DDX50	XP_850174.2
Mus musculus (house mouse)	Ddx50	NP_444413.1
Arabidopsis thaliana (thale cress)	emb1138	NP_001031943.1
Oryza sativa (rice)	Os03g0827700	NP_001051773.1
Homo sapiens (human)	DDX50	NP_076950.1
Bos taurus (cattle)	DDX50	NP_001192982.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DDX50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa34244	XM_015461003.1 Latest version!	Bos taurus DEAD-box helicase 50 (DDX50), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
OBa34244	XM_010820595.2 Latest version!	Bos taurus DEAD-box helicase 50 (DDX50), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
OBa34244	XM_005226430.3 Latest version!	Bos taurus DEAD-box helicase 50 (DDX50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
OBa39381	NM_001206053.1 Latest version!	Bos taurus DExD-box helicase 50 (DDX50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa238902	NM_001206053.2 Latest version!	Bos taurus DExD-box helicase 50 (DDX50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa231618	XM_010820595.3 Latest version!	Bos taurus DExD-box helicase 50 (DDX50), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa231618	XM_005226430.4 Latest version!	Bos taurus DExD-box helicase 50 (DDX50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa231618	XM_024986700.1 Latest version!	Bos taurus DExD-box helicase 50 (DDX50), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX50 ( XM_015461003.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316489.1 Bos taurus DEAD-box helicase 50 (DDX50), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa34244
Clone ID Related Accession (Same CDS sequence)	XM_015461003.1 , XM_005226430.3 , XM_010820595.2
Accession Version	XM_015461003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ATP-dependent RNA helicase DDX50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGCTAAACGG TGACACCGAG GAAGGATGTG ATAGACTTTC AGATGAATTT 
TCTAAATCTC ATAAGCCAAG AAGAAAAGAT CTATCAAATG GAGATATTGA TGAACATGAA 
AAAAGATCAA AGCGAGTGTC ATCTTTAAAT AGTTCTACTC ATAAATCAAG TGACAATAAA 
GTAGAAGAGA CCCTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT CCCTATTTCT 
GAAGAGACTA TAAAACTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC AATTCAAGTT 
AAGACTTTTG GTCCTGTATA CGAAGGAAAA GATTTAATTG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATTCCCTTG ATTGAAAGAC TCCAAAGAAA TCAAGAGACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CTACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATGTAACT AGGAAACTCA GTGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGG 
ACCCCAGGTC GTATCAAAGA CCATCTGCAG AGCGGCCGCT TAGATCTTTC TAAACTGCGC 
CACGTTGTGC TTGATGAAGT GGATCAAATG TTAGATTTAG GTTTTGCTGA ACAAGTTGAA 
GACATTCTCC ATGAGTCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTGCTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATATATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG GAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GCCACTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATCTTC TGTGAGACCA AAAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT CGCTCAGTCA 
CAAAGAGAAA TTACACTGAA AGGCTTCAGA GAGGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAGGTTGACC TGGTGATTCA GAGTTCACCT 
CCGCAGGATG TTGAGTCCTA CATTCATCGC TCTGGACGCA CAGGTCGAGC TGGCCGGACA 
GGGATCTGCA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AATTACGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTTTGGCT TCTGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAGGGGGCAG TGGATGCGCT GGCTGCAGCG 
TTAGCCCACA TCTCTGGCGC GTCAAGTTTT GAACCACGAT CTTTGATCAC CTCCGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCCA GAAGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCTGTGTCCC AGATTACCAG AATGTGCCTC 
CTAAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCCACAA CTGAATCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCAGA CTGGATACTC TCAGTGCCAG CCAAGTTGCC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGTTG GTCAAGTGGC 
CGGTTGGGCC GGTCAGGCCG GTCAGGTGGT CGATCCGGTG GCCGGTCAGG TAGACAGAGT 
CGGCAGGGGA GTCGGACAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAGCGGAAT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316489.1
CDS	1215..3233
Translation	MKEKLNGDTEEGCDRLSDEFSKSHKPRRKDLSNGDIDEHEKRSKRVSSLNSSTHKSSDNKVEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDVTRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDILHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESPEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRLGRSGRSGGRSGGRSGRQSRQGSRTGSRQDGRRRSGNRNRSRSGGHKRNFD

Target ORF information:

RefSeq Version	XM_015461003.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DEAD-box helicase 50 (DDX50), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461003.1

ORF Insert Sequence:

ATGAAAGAGA AGCTAAACGG TGACACCGAG GAAGGATGTG ATAGACTTTC AGATGAATTT 
TCTAAATCTC ATAAGCCAAG AAGAAAAGAT CTATCAAATG GAGATATTGA TGAACATGAA 
AAAAGATCAA AGCGAGTGTC ATCTTTAAAT AGTTCTACTC ATAAATCAAG TGACAATAAA 
GTAGAAGAGA CCCTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT CCCTATTTCT 
GAAGAGACTA TAAAACTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC AATTCAAGTT 
AAGACTTTTG GTCCTGTATA CGAAGGAAAA GATTTAATTG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATTCCCTTG ATTGAAAGAC TCCAAAGAAA TCAAGAGACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CTACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATGTAACT AGGAAACTCA GTGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGG 
ACCCCAGGTC GTATCAAAGA CCATCTGCAG AGCGGCCGCT TAGATCTTTC TAAACTGCGC 
CACGTTGTGC TTGATGAAGT GGATCAAATG TTAGATTTAG GTTTTGCTGA ACAAGTTGAA 
GACATTCTCC ATGAGTCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTGCTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATATATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG GAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GCCACTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATCTTC TGTGAGACCA AAAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT CGCTCAGTCA 
CAAAGAGAAA TTACACTGAA AGGCTTCAGA GAGGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAGGTTGACC TGGTGATTCA GAGTTCACCT 
CCGCAGGATG TTGAGTCCTA CATTCATCGC TCTGGACGCA CAGGTCGAGC TGGCCGGACA 
GGGATCTGCA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AATTACGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTTTGGCT TCTGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAGGGGGCAG TGGATGCGCT GGCTGCAGCG 
TTAGCCCACA TCTCTGGCGC GTCAAGTTTT GAACCACGAT CTTTGATCAC CTCCGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCCA GAAGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCTGTGTCCC AGATTACCAG AATGTGCCTC 
CTAAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCCACAA CTGAATCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCAGA CTGGATACTC TCAGTGCCAG CCAAGTTGCC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGTTG GTCAAGTGGC 
CGGTTGGGCC GGTCAGGCCG GTCAGGTGGT CGATCCGGTG GCCGGTCAGG TAGACAGAGT 
CGGCAGGGGA GTCGGACAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAGCGGAAT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX50 ( XM_005226430.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226487.1 Bos taurus DEAD-box helicase 50 (DDX50), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa34244
Clone ID Related Accession (Same CDS sequence)	XM_015461003.1 , XM_005226430.3 , XM_010820595.2
Accession Version	XM_005226430.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ATP-dependent RNA helicase DDX50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226430.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGCTAAACGG TGACACCGAG GAAGGATGTG ATAGACTTTC AGATGAATTT 
TCTAAATCTC ATAAGCCAAG AAGAAAAGAT CTATCAAATG GAGATATTGA TGAACATGAA 
AAAAGATCAA AGCGAGTGTC ATCTTTAAAT AGTTCTACTC ATAAATCAAG TGACAATAAA 
GTAGAAGAGA CCCTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT CCCTATTTCT 
GAAGAGACTA TAAAACTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC AATTCAAGTT 
AAGACTTTTG GTCCTGTATA CGAAGGAAAA GATTTAATTG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATTCCCTTG ATTGAAAGAC TCCAAAGAAA TCAAGAGACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CTACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATGTAACT AGGAAACTCA GTGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGG 
ACCCCAGGTC GTATCAAAGA CCATCTGCAG AGCGGCCGCT TAGATCTTTC TAAACTGCGC 
CACGTTGTGC TTGATGAAGT GGATCAAATG TTAGATTTAG GTTTTGCTGA ACAAGTTGAA 
GACATTCTCC ATGAGTCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTGCTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATATATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG GAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GCCACTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATCTTC TGTGAGACCA AAAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT CGCTCAGTCA 
CAAAGAGAAA TTACACTGAA AGGCTTCAGA GAGGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAGGTTGACC TGGTGATTCA GAGTTCACCT 
CCGCAGGATG TTGAGTCCTA CATTCATCGC TCTGGACGCA CAGGTCGAGC TGGCCGGACA 
GGGATCTGCA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AATTACGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTTTGGCT TCTGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAGGGGGCAG TGGATGCGCT GGCTGCAGCG 
TTAGCCCACA TCTCTGGCGC GTCAAGTTTT GAACCACGAT CTTTGATCAC CTCCGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCCA GAAGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCTGTGTCCC AGATTACCAG AATGTGCCTC 
CTAAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCCACAA CTGAATCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCAGA CTGGATACTC TCAGTGCCAG CCAAGTTGCC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGTTG GTCAAGTGGC 
CGGTTGGGCC GGTCAGGCCG GTCAGGTGGT CGATCCGGTG GCCGGTCAGG TAGACAGAGT 
CGGCAGGGGA GTCGGACAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAGCGGAAT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226487.1
CDS	455..2473
Translation	MKEKLNGDTEEGCDRLSDEFSKSHKPRRKDLSNGDIDEHEKRSKRVSSLNSSTHKSSDNKVEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDVTRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDILHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESPEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRLGRSGRSGGRSGGRSGRQSRQGSRTGSRQDGRRRSGNRNRSRSGGHKRNFD

Target ORF information:

RefSeq Version	XM_005226430.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DEAD-box helicase 50 (DDX50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226430.3

ORF Insert Sequence:

ATGAAAGAGA AGCTAAACGG TGACACCGAG GAAGGATGTG ATAGACTTTC AGATGAATTT 
TCTAAATCTC ATAAGCCAAG AAGAAAAGAT CTATCAAATG GAGATATTGA TGAACATGAA 
AAAAGATCAA AGCGAGTGTC ATCTTTAAAT AGTTCTACTC ATAAATCAAG TGACAATAAA 
GTAGAAGAGA CCCTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT CCCTATTTCT 
GAAGAGACTA TAAAACTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC AATTCAAGTT 
AAGACTTTTG GTCCTGTATA CGAAGGAAAA GATTTAATTG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATTCCCTTG ATTGAAAGAC TCCAAAGAAA TCAAGAGACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CTACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATGTAACT AGGAAACTCA GTGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGG 
ACCCCAGGTC GTATCAAAGA CCATCTGCAG AGCGGCCGCT TAGATCTTTC TAAACTGCGC 
CACGTTGTGC TTGATGAAGT GGATCAAATG TTAGATTTAG GTTTTGCTGA ACAAGTTGAA 
GACATTCTCC ATGAGTCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTGCTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATATATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG GAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GCCACTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATCTTC TGTGAGACCA AAAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT CGCTCAGTCA 
CAAAGAGAAA TTACACTGAA AGGCTTCAGA GAGGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAGGTTGACC TGGTGATTCA GAGTTCACCT 
CCGCAGGATG TTGAGTCCTA CATTCATCGC TCTGGACGCA CAGGTCGAGC TGGCCGGACA 
GGGATCTGCA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AATTACGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTTTGGCT TCTGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAGGGGGCAG TGGATGCGCT GGCTGCAGCG 
TTAGCCCACA TCTCTGGCGC GTCAAGTTTT GAACCACGAT CTTTGATCAC CTCCGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCCA GAAGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCTGTGTCCC AGATTACCAG AATGTGCCTC 
CTAAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCCACAA CTGAATCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCAGA CTGGATACTC TCAGTGCCAG CCAAGTTGCC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGTTG GTCAAGTGGC 
CGGTTGGGCC GGTCAGGCCG GTCAGGTGGT CGATCCGGTG GCCGGTCAGG TAGACAGAGT 
CGGCAGGGGA GTCGGACAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAGCGGAAT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX50 ( XM_010820595.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818897.1 Bos taurus DEAD-box helicase 50 (DDX50), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa34244
Clone ID Related Accession (Same CDS sequence)	XM_015461003.1 , XM_005226430.3 , XM_010820595.2
Accession Version	XM_010820595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ATP-dependent RNA helicase DDX50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGCTAAACGG TGACACCGAG GAAGGATGTG ATAGACTTTC AGATGAATTT 
TCTAAATCTC ATAAGCCAAG AAGAAAAGAT CTATCAAATG GAGATATTGA TGAACATGAA 
AAAAGATCAA AGCGAGTGTC ATCTTTAAAT AGTTCTACTC ATAAATCAAG TGACAATAAA 
GTAGAAGAGA CCCTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT CCCTATTTCT 
GAAGAGACTA TAAAACTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC AATTCAAGTT 
AAGACTTTTG GTCCTGTATA CGAAGGAAAA GATTTAATTG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATTCCCTTG ATTGAAAGAC TCCAAAGAAA TCAAGAGACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CTACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATGTAACT AGGAAACTCA GTGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGG 
ACCCCAGGTC GTATCAAAGA CCATCTGCAG AGCGGCCGCT TAGATCTTTC TAAACTGCGC 
CACGTTGTGC TTGATGAAGT GGATCAAATG TTAGATTTAG GTTTTGCTGA ACAAGTTGAA 
GACATTCTCC ATGAGTCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTGCTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATATATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG GAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GCCACTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATCTTC TGTGAGACCA AAAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT CGCTCAGTCA 
CAAAGAGAAA TTACACTGAA AGGCTTCAGA GAGGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAGGTTGACC TGGTGATTCA GAGTTCACCT 
CCGCAGGATG TTGAGTCCTA CATTCATCGC TCTGGACGCA CAGGTCGAGC TGGCCGGACA 
GGGATCTGCA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AATTACGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTTTGGCT TCTGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAGGGGGCAG TGGATGCGCT GGCTGCAGCG 
TTAGCCCACA TCTCTGGCGC GTCAAGTTTT GAACCACGAT CTTTGATCAC CTCCGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCCA GAAGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCTGTGTCCC AGATTACCAG AATGTGCCTC 
CTAAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCCACAA CTGAATCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCAGA CTGGATACTC TCAGTGCCAG CCAAGTTGCC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGTTG GTCAAGTGGC 
CGGTTGGGCC GGTCAGGCCG GTCAGGTGGT CGATCCGGTG GCCGGTCAGG TAGACAGAGT 
CGGCAGGGGA GTCGGACAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAGCGGAAT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818897.1
CDS	1103..3121
Translation	MKEKLNGDTEEGCDRLSDEFSKSHKPRRKDLSNGDIDEHEKRSKRVSSLNSSTHKSSDNKVEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDVTRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDILHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESPEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRLGRSGRSGGRSGGRSGRQSRQGSRTGSRQDGRRRSGNRNRSRSGGHKRNFD

Target ORF information:

RefSeq Version	XM_010820595.2
Organism	Bos taurus(cattle)
Definition	Bos taurus DEAD-box helicase 50 (DDX50), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820595.2

ORF Insert Sequence:

ATGAAAGAGA AGCTAAACGG TGACACCGAG GAAGGATGTG ATAGACTTTC AGATGAATTT 
TCTAAATCTC ATAAGCCAAG AAGAAAAGAT CTATCAAATG GAGATATTGA TGAACATGAA 
AAAAGATCAA AGCGAGTGTC ATCTTTAAAT AGTTCTACTC ATAAATCAAG TGACAATAAA 
GTAGAAGAGA CCCTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT CCCTATTTCT 
GAAGAGACTA TAAAACTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC AATTCAAGTT 
AAGACTTTTG GTCCTGTATA CGAAGGAAAA GATTTAATTG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATTCCCTTG ATTGAAAGAC TCCAAAGAAA TCAAGAGACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CTACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATGTAACT AGGAAACTCA GTGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGG 
ACCCCAGGTC GTATCAAAGA CCATCTGCAG AGCGGCCGCT TAGATCTTTC TAAACTGCGC 
CACGTTGTGC TTGATGAAGT GGATCAAATG TTAGATTTAG GTTTTGCTGA ACAAGTTGAA 
GACATTCTCC ATGAGTCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTGCTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATATATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG GAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GCCACTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATCTTC TGTGAGACCA AAAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT CGCTCAGTCA 
CAAAGAGAAA TTACACTGAA AGGCTTCAGA GAGGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAGGTTGACC TGGTGATTCA GAGTTCACCT 
CCGCAGGATG TTGAGTCCTA CATTCATCGC TCTGGACGCA CAGGTCGAGC TGGCCGGACA 
GGGATCTGCA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AATTACGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTTTGGCT TCTGTTTCTT ATGCTGCTGT TGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAGGGGGCAG TGGATGCGCT GGCTGCAGCG 
TTAGCCCACA TCTCTGGCGC GTCAAGTTTT GAACCACGAT CTTTGATCAC CTCCGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCCA GAAGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCTGTGTCCC AGATTACCAG AATGTGCCTC 
CTAAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCCACAA CTGAATCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCAGA CTGGATACTC TCAGTGCCAG CCAAGTTGCC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGTTG GTCAAGTGGC 
CGGTTGGGCC GGTCAGGCCG GTCAGGTGGT CGATCCGGTG GCCGGTCAGG TAGACAGAGT 
CGGCAGGGGA GTCGGACAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAGCGGAAT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX50 ( NM_001206053.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192982.1 Bos taurus DExD-box helicase 50 (DDX50), mRNA.

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CloneID	OBa39381
Clone ID Related Accession (Same CDS sequence)	NM_001206053.1
Accession Version	NM_001206053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	ATP-dependent RNA helicase DDX50
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02061828.1. On Apr 20, 2011 this sequence version replaced XM_614067.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00120237, SAMN02403641 [ECO:0000348] ##Evidence-Data-END##

ATGCCTGGGA AACTCCTCTG GGGGGACATT ATGGAGCTGG AGGCACCCTT GGAGGAGACC 
GAGAGCCAGA GGAAGGAGAG GCAAAAGAGT GATAGAAGGA AGTCAAGGCA CCATTATGAC 
TCAGATGAGA AATCAGAAAC AAGAGAAAAT GGTGTTACAG ATGACTTGGA TGCTCCCAAA 
CCCAAAAAAG CTAAAATGAA AGAGAAGCTA AACGGTGACA CCGAGGAAGG ATGTGATAGA 
CTTTCAGATG AATTTTCTAA ATCTCATAAG CCAAGAAGAA AAGATCTATC AAATGGAGAT 
ATTGATGAAC ATGAAAAAAG ATCAAAGCGA GTGTCATCTT TAAATAGTTC TACTCATAAA 
TCAAGTGACA ATAAAGTAGA AGAGACCCTA ACACGTGAAC AGAAAGAAGG AGCCTTCTCC 
AATTTCCCTA TTTCTGAAGA GACTATAAAA CTTCTGAAAG GTCGAGGGGT AACATATCTC 
TTTCCAATTC AAGTTAAGAC TTTTGGTCCT GTATACGAAG GAAAAGATTT AATTGCTCAA 
GCACGGACAG GAACAGGAAA GACATTCTCT TTTGCCATTC CCTTGATTGA AAGACTCCAA 
AGAAATCAAG AGACAATTAA AAAAAGCCGC TCACCAAAGG TACTTGTTTT GGCTCCTACA 
AGGGAACTGG CAAACCAAGT AGCCAAAGAC TTCAAAGATG TAACTAGGAA ACTCAGTGTG 
GCGTGTTTTT ATGGTGGAAC ATCATATCAA AGCCAAATTA ATCATATTCG AAATGGTATT 
GACATCTTGG TTGGGACCCC AGGTCGTATC AAAGACCATC TGCAGAGCGG CCGCTTAGAT 
CTTTCTAAAC TGCGCCACGT TGTGCTTGAT GAAGTGGATC AAATGTTAGA TTTAGGTTTT 
GCTGAACAAG TTGAAGACAT TCTCCATGAG TCCTACAAAA CTGATTCTGA AGACAATCCT 
CAGACTTTGC TTTTTTCTGC AACTTGCCCA CAGTGGGTAT ACAAAGTTGC AAAAAAATAT 
ATGAAATCCA GATATGAACA GGTTGACCTT GTTGGGAAAA TGACTCAAAA GGCTGCAACT 
ACTGTGGAAC ATTTGGCCAT CCAGTGCCAC TGGTCTCAGA GGCCAGCAGT TATTGGAGAT 
GTCCTTCAAG TCTACAGTGG GTCTGAAGGG AGGGCTATTA TCTTCTGTGA GACCAAAAAG 
AATGTAACTG AAATGGCCAT GAATCCACAC ATAAAACAGA ATGCCCAGTG TTTACATGGG 
GACATCGCTC AGTCACAAAG AGAAATTACA CTGAAAGGCT TCAGAGAGGG TAGTTTTAAA 
GTTTTGGTGG CAACCAATGT GGCTGCCCGT GGTTTGGACA TTCCTGAGGT TGACCTGGTG 
ATTCAGAGTT CACCTCCGCA GGATGTTGAG TCCTACATTC ATCGCTCTGG ACGCACAGGT 
CGAGCTGGCC GGACAGGGAT CTGCATATGT TTTTATCAAC CAAGAGAAAG AGGTCAATTA 
CGATATGTGG AACAAAAAGC AGGAATTACT TTTAAACGTG TAGGTGTTCC TTCTACAATG 
GATTTAGTTA AATCTAAAAG CATGGATGCC ATCAGGTCTT TGGCTTCTGT TTCTTATGCT 
GCTGTTGATT TTTTCCGACC ATCAGCTCAG AGACTGATAG AAGAGAAGGG GGCAGTGGAT 
GCGCTGGCTG CAGCGTTAGC CCACATCTCT GGCGCGTCAA GTTTTGAACC ACGATCTTTG 
ATCACCTCCG ATAAGGGGTT TGTGACCATG ACTCTGGAAA GCCCAGAAGA AATACAGGAT 
GTCAGCTGTG CTTGGAAAGA ACTTAACAGA AAGCTGAGTA GTAATGCTGT GTCCCAGATT 
ACCAGAATGT GCCTCCTAAA AGGAAATATG GGTGTTTGCT TTGATGTTCC CACAACTGAA 
TCAGAAAGGT TACAGGCAGA GTGGCATGAT TCAGACTGGA TACTCTCAGT GCCAGCCAAG 
TTGCCTGAAA TTGAAGAATA TTATGATGGA AACACATCTT CTAATTCCAG ACAGAGGAGT 
GGTTGGTCAA GTGGCCGGTT GGGCCGGTCA GGCCGGTCAG GTGGTCGATC CGGTGGCCGG 
TCAGGTAGAC AGAGTCGGCA GGGGAGTCGG ACAGGAAGTC GACAAGATGG TAGAAGACGA 
AGTGGGAATA GAAATCGATC AAGAAGTGGG GGCCACAAGC GGAATTTTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192982.1
CDS	123..2336
Translation	MPGKLLWGDIMELEAPLEETESQRKERQKSDRRKSRHHYDSDEKSETRENGVTDDLDAPKPKKAKMKEKLNGDTEEGCDRLSDEFSKSHKPRRKDLSNGDIDEHEKRSKRVSSLNSSTHKSSDNKVEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDVTRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDILHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESPEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRLGRSGRSGGRSGGRSGRQSRQGSRTGSRQDGRRRSGNRNRSRSGGHKRNFD

Target ORF information:

RefSeq Version	NM_001206053.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DExD-box helicase 50 (DDX50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206053.1

ORF Insert Sequence:

ATGCCTGGGA AACTCCTCTG GGGGGACATT ATGGAGCTGG AGGCACCCTT GGAGGAGACC 
GAGAGCCAGA GGAAGGAGAG GCAAAAGAGT GATAGAAGGA AGTCAAGGCA CCATTATGAC 
TCAGATGAGA AATCAGAAAC AAGAGAAAAT GGTGTTACAG ATGACTTGGA TGCTCCCAAA 
CCCAAAAAAG CTAAAATGAA AGAGAAGCTA AACGGTGACA CCGAGGAAGG ATGTGATAGA 
CTTTCAGATG AATTTTCTAA ATCTCATAAG CCAAGAAGAA AAGATCTATC AAATGGAGAT 
ATTGATGAAC ATGAAAAAAG ATCAAAGCGA GTGTCATCTT TAAATAGTTC TACTCATAAA 
TCAAGTGACA ATAAAGTAGA AGAGACCCTA ACACGTGAAC AGAAAGAAGG AGCCTTCTCC 
AATTTCCCTA TTTCTGAAGA GACTATAAAA CTTCTGAAAG GTCGAGGGGT AACATATCTC 
TTTCCAATTC AAGTTAAGAC TTTTGGTCCT GTATACGAAG GAAAAGATTT AATTGCTCAA 
GCACGGACAG GAACAGGAAA GACATTCTCT TTTGCCATTC CCTTGATTGA AAGACTCCAA 
AGAAATCAAG AGACAATTAA AAAAAGCCGC TCACCAAAGG TACTTGTTTT GGCTCCTACA 
AGGGAACTGG CAAACCAAGT AGCCAAAGAC TTCAAAGATG TAACTAGGAA ACTCAGTGTG 
GCGTGTTTTT ATGGTGGAAC ATCATATCAA AGCCAAATTA ATCATATTCG AAATGGTATT 
GACATCTTGG TTGGGACCCC AGGTCGTATC AAAGACCATC TGCAGAGCGG CCGCTTAGAT 
CTTTCTAAAC TGCGCCACGT TGTGCTTGAT GAAGTGGATC AAATGTTAGA TTTAGGTTTT 
GCTGAACAAG TTGAAGACAT TCTCCATGAG TCCTACAAAA CTGATTCTGA AGACAATCCT 
CAGACTTTGC TTTTTTCTGC AACTTGCCCA CAGTGGGTAT ACAAAGTTGC AAAAAAATAT 
ATGAAATCCA GATATGAACA GGTTGACCTT GTTGGGAAAA TGACTCAAAA GGCTGCAACT 
ACTGTGGAAC ATTTGGCCAT CCAGTGCCAC TGGTCTCAGA GGCCAGCAGT TATTGGAGAT 
GTCCTTCAAG TCTACAGTGG GTCTGAAGGG AGGGCTATTA TCTTCTGTGA GACCAAAAAG 
AATGTAACTG AAATGGCCAT GAATCCACAC ATAAAACAGA ATGCCCAGTG TTTACATGGG 
GACATCGCTC AGTCACAAAG AGAAATTACA CTGAAAGGCT TCAGAGAGGG TAGTTTTAAA 
GTTTTGGTGG CAACCAATGT GGCTGCCCGT GGTTTGGACA TTCCTGAGGT TGACCTGGTG 
ATTCAGAGTT CACCTCCGCA GGATGTTGAG TCCTACATTC ATCGCTCTGG ACGCACAGGT 
CGAGCTGGCC GGACAGGGAT CTGCATATGT TTTTATCAAC CAAGAGAAAG AGGTCAATTA 
CGATATGTGG AACAAAAAGC AGGAATTACT TTTAAACGTG TAGGTGTTCC TTCTACAATG 
GATTTAGTTA AATCTAAAAG CATGGATGCC ATCAGGTCTT TGGCTTCTGT TTCTTATGCT 
GCTGTTGATT TTTTCCGACC ATCAGCTCAG AGACTGATAG AAGAGAAGGG GGCAGTGGAT 
GCGCTGGCTG CAGCGTTAGC CCACATCTCT GGCGCGTCAA GTTTTGAACC ACGATCTTTG 
ATCACCTCCG ATAAGGGGTT TGTGACCATG ACTCTGGAAA GCCCAGAAGA AATACAGGAT 
GTCAGCTGTG CTTGGAAAGA ACTTAACAGA AAGCTGAGTA GTAATGCTGT GTCCCAGATT 
ACCAGAATGT GCCTCCTAAA AGGAAATATG GGTGTTTGCT TTGATGTTCC CACAACTGAA 
TCAGAAAGGT TACAGGCAGA GTGGCATGAT TCAGACTGGA TACTCTCAGT GCCAGCCAAG 
TTGCCTGAAA TTGAAGAATA TTATGATGGA AACACATCTT CTAATTCCAG ACAGAGGAGT 
GGTTGGTCAA GTGGCCGGTT GGGCCGGTCA GGCCGGTCAG GTGGTCGATC CGGTGGCCGG 
TCAGGTAGAC AGAGTCGGCA GGGGAGTCGG ACAGGAAGTC GACAAGATGG TAGAAGACGA 
AGTGGGAATA GAAATCGATC AAGAAGTGGG GGCCACAAGC GGAATTTTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX50 ( NM_001206053.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192982.1 Bos taurus DExD-box helicase 50 (DDX50), mRNA.

Price & Availability↑

CloneID	OBa238902
Clone ID Related Accession (Same CDS sequence)	NM_001206053.2
Accession Version	NM_001206053.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	ATP-dependent RNA helicase DDX50
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000028.1. On May 29, 2018 this sequence version replaced NM_001206053.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.10783.1, SRR5571254.22706.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGCCTGGGA AACTCCTCTG GGGGGACATT ATGGAGCTGG AGGCACCCTT GGAGGAGACC 
GAGAGCCAGA GGAAGGAGAG GCAAAAGAGT GATAGAAGGA AGTCAAGGCA CCATTATGAC 
TCAGATGAGA AATCAGAAAC AAGAGAAAAT GGTGTTACAG ATGACTTGGA TGCTCCCAAA 
CCCAAAAAAG CTAAAATGAA AGAGAAGCTA AACGGTGACA CCGAGGAAGG ATGTGATAGA 
CTTTCAGATG AATTTTCTAA ATCTCATAAG CCAAGAAGAA AAGATCTATC AAATGGAGAT 
ATTGATGAAC ATGAAAAAAG ATCAAAGCGA GTGTCATCTT TAAATAGTTC TACTCATAAA 
TCAAGTGACA ATAAAGTAGA AGAGACCCTA ACACGTGAAC AGAAAGAAGG AGCCTTCTCC 
AATTTCCCTA TTTCTGAAGA GACTATAAAA CTTCTGAAAG GTCGAGGGGT AACATATCTC 
TTTCCAATTC AAGTTAAGAC TTTTGGTCCT GTATACGAAG GAAAAGATTT AATTGCTCAA 
GCACGGACAG GAACAGGAAA GACATTCTCT TTTGCCATTC CCTTGATTGA AAGACTCCAA 
AGAAATCAAG AGACAATTAA AAAAAGCCGC TCACCAAAGG TACTTGTTTT GGCTCCTACA 
AGGGAACTGG CAAACCAAGT AGCCAAAGAC TTCAAAGATG TAACTAGGAA ACTCAGTGTG 
GCGTGTTTTT ATGGTGGAAC ATCATATCAA AGCCAAATTA ATCATATTCG AAATGGTATT 
GACATCTTGG TTGGGACCCC AGGTCGTATC AAAGACCATC TGCAGAGCGG CCGCTTAGAT 
CTTTCTAAAC TGCGCCACGT TGTGCTTGAT GAAGTGGATC AAATGTTAGA TTTAGGTTTT 
GCTGAACAAG TTGAAGACAT TCTCCATGAG TCCTACAAAA CTGATTCTGA AGACAATCCT 
CAGACTTTGC TTTTTTCTGC AACTTGCCCA CAGTGGGTAT ACAAAGTTGC AAAAAAATAT 
ATGAAATCCA GATATGAACA GGTTGACCTT GTTGGGAAAA TGACTCAAAA GGCTGCAACT 
ACTGTGGAAC ATTTGGCCAT CCAGTGCCAC TGGTCTCAGA GGCCAGCAGT TATTGGAGAT 
GTCCTTCAAG TCTACAGTGG GTCTGAAGGG AGGGCTATTA TCTTCTGTGA GACCAAAAAG 
AATGTAACTG AAATGGCCAT GAATCCACAC ATAAAACAGA ATGCCCAGTG TTTACATGGG 
GACATCGCTC AGTCACAAAG AGAAATTACA CTGAAAGGCT TCAGAGAGGG TAGTTTTAAA 
GTTTTGGTGG CAACCAATGT GGCTGCCCGT GGTTTGGACA TTCCTGAGGT TGACCTGGTG 
ATTCAGAGTT CACCTCCGCA GGATGTTGAG TCCTACATTC ATCGCTCTGG ACGCACAGGT 
CGAGCTGGCC GGACAGGGAT CTGCATATGT TTTTATCAAC CAAGAGAAAG AGGTCAATTA 
CGATATGTGG AACAAAAAGC AGGAATTACT TTTAAACGTG TAGGTGTTCC TTCTACAATG 
GATTTAGTTA AATCTAAAAG CATGGATGCC ATCAGGTCTT TGGCTTCTGT TTCTTATGCT 
GCTGTCGATT TTTTCCGACC ATCAGCTCAG AGACTGATAG AAGAGAAGGG GGCAGTGGAT 
GCGCTGGCTG CAGCGTTAGC CCACATCTCT GGCGCGTCAA GTTTTGAACC ACGATCTTTG 
ATCACCTCCG ATAAGGGGTT TGTGACCATG ACTCTGGAAA GCCCAGAAGA AATACAGGAT 
GTCAGCTGTG CTTGGAAAGA ACTTAACAGA AAGCTGAGTA GTAATGCTGT GTCCCAGATT 
ACCAGAATGT GCCTCCTAAA AGGAAATATG GGTGTTTGCT TTGATGTTCC CACAACTGAA 
TCAGAAAGGT TACAGGCAGA GTGGCATGAT TCAGACTGGA TACTCTCAGT GCCAGCCAAG 
TTGCCTGAAA TTGAAGAATA TTATGATGGA AACACATCTT CTAATTCCAG ACAGAGGAGT 
GGTTGGTCAA GTGGCCGGTT GGGCCGGTCA GGCCGGTCAG GTGGTCGATC CGGTGGCCGG 
TCAGGTAGAC AGAGTCGGCA GGGGAGTCGG ACAGGAAGTC GACAAGATGG TAGAAGACGA 
AGTGGGAATA GAAATCGATC AAGAAGTGGG GGCCACAAGC GGAATTTTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192982.1
CDS	123..2336
Translation	MPGKLLWGDIMELEAPLEETESQRKERQKSDRRKSRHHYDSDEKSETRENGVTDDLDAPKPKKAKMKEKLNGDTEEGCDRLSDEFSKSHKPRRKDLSNGDIDEHEKRSKRVSSLNSSTHKSSDNKVEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDVTRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDILHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESPEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRLGRSGRSGGRSGGRSGRQSRQGSRTGSRQDGRRRSGNRNRSRSGGHKRNFD

Target ORF information:

RefSeq Version	NM_001206053.2
Organism	Bos taurus(cattle)
Definition	Bos taurus DExD-box helicase 50 (DDX50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206053.2

ORF Insert Sequence:

ATGCCTGGGA AACTCCTCTG GGGGGACATT ATGGAGCTGG AGGCACCCTT GGAGGAGACC 
GAGAGCCAGA GGAAGGAGAG GCAAAAGAGT GATAGAAGGA AGTCAAGGCA CCATTATGAC 
TCAGATGAGA AATCAGAAAC AAGAGAAAAT GGTGTTACAG ATGACTTGGA TGCTCCCAAA 
CCCAAAAAAG CTAAAATGAA AGAGAAGCTA AACGGTGACA CCGAGGAAGG ATGTGATAGA 
CTTTCAGATG AATTTTCTAA ATCTCATAAG CCAAGAAGAA AAGATCTATC AAATGGAGAT 
ATTGATGAAC ATGAAAAAAG ATCAAAGCGA GTGTCATCTT TAAATAGTTC TACTCATAAA 
TCAAGTGACA ATAAAGTAGA AGAGACCCTA ACACGTGAAC AGAAAGAAGG AGCCTTCTCC 
AATTTCCCTA TTTCTGAAGA GACTATAAAA CTTCTGAAAG GTCGAGGGGT AACATATCTC 
TTTCCAATTC AAGTTAAGAC TTTTGGTCCT GTATACGAAG GAAAAGATTT AATTGCTCAA 
GCACGGACAG GAACAGGAAA GACATTCTCT TTTGCCATTC CCTTGATTGA AAGACTCCAA 
AGAAATCAAG AGACAATTAA AAAAAGCCGC TCACCAAAGG TACTTGTTTT GGCTCCTACA 
AGGGAACTGG CAAACCAAGT AGCCAAAGAC TTCAAAGATG TAACTAGGAA ACTCAGTGTG 
GCGTGTTTTT ATGGTGGAAC ATCATATCAA AGCCAAATTA ATCATATTCG AAATGGTATT 
GACATCTTGG TTGGGACCCC AGGTCGTATC AAAGACCATC TGCAGAGCGG CCGCTTAGAT 
CTTTCTAAAC TGCGCCACGT TGTGCTTGAT GAAGTGGATC AAATGTTAGA TTTAGGTTTT 
GCTGAACAAG TTGAAGACAT TCTCCATGAG TCCTACAAAA CTGATTCTGA AGACAATCCT 
CAGACTTTGC TTTTTTCTGC AACTTGCCCA CAGTGGGTAT ACAAAGTTGC AAAAAAATAT 
ATGAAATCCA GATATGAACA GGTTGACCTT GTTGGGAAAA TGACTCAAAA GGCTGCAACT 
ACTGTGGAAC ATTTGGCCAT CCAGTGCCAC TGGTCTCAGA GGCCAGCAGT TATTGGAGAT 
GTCCTTCAAG TCTACAGTGG GTCTGAAGGG AGGGCTATTA TCTTCTGTGA GACCAAAAAG 
AATGTAACTG AAATGGCCAT GAATCCACAC ATAAAACAGA ATGCCCAGTG TTTACATGGG 
GACATCGCTC AGTCACAAAG AGAAATTACA CTGAAAGGCT TCAGAGAGGG TAGTTTTAAA 
GTTTTGGTGG CAACCAATGT GGCTGCCCGT GGTTTGGACA TTCCTGAGGT TGACCTGGTG 
ATTCAGAGTT CACCTCCGCA GGATGTTGAG TCCTACATTC ATCGCTCTGG ACGCACAGGT 
CGAGCTGGCC GGACAGGGAT CTGCATATGT TTTTATCAAC CAAGAGAAAG AGGTCAATTA 
CGATATGTGG AACAAAAAGC AGGAATTACT TTTAAACGTG TAGGTGTTCC TTCTACAATG 
GATTTAGTTA AATCTAAAAG CATGGATGCC ATCAGGTCTT TGGCTTCTGT TTCTTATGCT 
GCTGTCGATT TTTTCCGACC ATCAGCTCAG AGACTGATAG AAGAGAAGGG GGCAGTGGAT 
GCGCTGGCTG CAGCGTTAGC CCACATCTCT GGCGCGTCAA GTTTTGAACC ACGATCTTTG 
ATCACCTCCG ATAAGGGGTT TGTGACCATG ACTCTGGAAA GCCCAGAAGA AATACAGGAT 
GTCAGCTGTG CTTGGAAAGA ACTTAACAGA AAGCTGAGTA GTAATGCTGT GTCCCAGATT 
ACCAGAATGT GCCTCCTAAA AGGAAATATG GGTGTTTGCT TTGATGTTCC CACAACTGAA 
TCAGAAAGGT TACAGGCAGA GTGGCATGAT TCAGACTGGA TACTCTCAGT GCCAGCCAAG 
TTGCCTGAAA TTGAAGAATA TTATGATGGA AACACATCTT CTAATTCCAG ACAGAGGAGT 
GGTTGGTCAA GTGGCCGGTT GGGCCGGTCA GGCCGGTCAG GTGGTCGATC CGGTGGCCGG 
TCAGGTAGAC AGAGTCGGCA GGGGAGTCGG ACAGGAAGTC GACAAGATGG TAGAAGACGA 
AGTGGGAATA GAAATCGATC AAGAAGTGGG GGCCACAAGC GGAATTTTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX50 ( XM_024986700.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842468.1 Bos taurus DExD-box helicase 50 (DDX50), transcript variant X2, mRNA.

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CloneID	OBa231618
Clone ID Related Accession (Same CDS sequence)	XM_024986700.1 , XM_010820595.3 , XM_005226430.4
Accession Version	XM_024986700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ATP-dependent RNA helicase DDX50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGCTAAACGG TGACACCGAG GAAGGATGTG ATAGACTTTC AGATGAATTT 
TCTAAATCTC ATAAGCCAAG AAGAAAAGAT CTATCAAATG GAGATATTGA TGAACATGAA 
AAAAGATCAA AGCGAGTGTC ATCTTTAAAT AGTTCTACTC ATAAATCAAG TGACAATAAA 
GTAGAAGAGA CCCTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT CCCTATTTCT 
GAAGAGACTA TAAAACTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC AATTCAAGTT 
AAGACTTTTG GTCCTGTATA CGAAGGAAAA GATTTAATTG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATTCCCTTG ATTGAAAGAC TCCAAAGAAA TCAAGAGACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CTACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATGTAACT AGGAAACTCA GTGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGG 
ACCCCAGGTC GTATCAAAGA CCATCTGCAG AGCGGCCGCT TAGATCTTTC TAAACTGCGC 
CACGTTGTGC TTGATGAAGT GGATCAAATG TTAGATTTAG GTTTTGCTGA ACAAGTTGAA 
GACATTCTCC ATGAGTCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTGCTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATATATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG GAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GCCACTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATCTTC TGTGAGACCA AAAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT CGCTCAGTCA 
CAAAGAGAAA TTACACTGAA AGGCTTCAGA GAGGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAGGTTGACC TGGTGATTCA GAGTTCACCT 
CCGCAGGATG TTGAGTCCTA CATTCATCGC TCTGGACGCA CAGGTCGAGC TGGCCGGACA 
GGGATCTGCA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AATTACGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTTTGGCT TCTGTTTCTT ATGCTGCTGT CGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAGGGGGCAG TGGATGCGCT GGCTGCAGCG 
TTAGCCCACA TCTCTGGCGC GTCAAGTTTT GAACCACGAT CTTTGATCAC CTCCGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCCA GAAGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCTGTGTCCC AGATTACCAG AATGTGCCTC 
CTAAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCCACAA CTGAATCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCAGA CTGGATACTC TCAGTGCCAG CCAAGTTGCC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGTTG GTCAAGTGGC 
CGGTTGGGCC GGTCAGGCCG GTCAGGTGGT CGATCCGGTG GCCGGTCAGG TAGACAGAGT 
CGGCAGGGGA GTCGGACAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAGCGGAAT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842468.1
CDS	435..2453
Translation	MKEKLNGDTEEGCDRLSDEFSKSHKPRRKDLSNGDIDEHEKRSKRVSSLNSSTHKSSDNKVEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDVTRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDILHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESPEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRLGRSGRSGGRSGGRSGRQSRQGSRTGSRQDGRRRSGNRNRSRSGGHKRNFD

Target ORF information:

RefSeq Version	XM_024986700.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DExD-box helicase 50 (DDX50), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986700.1

ORF Insert Sequence:

ATGAAAGAGA AGCTAAACGG TGACACCGAG GAAGGATGTG ATAGACTTTC AGATGAATTT 
TCTAAATCTC ATAAGCCAAG AAGAAAAGAT CTATCAAATG GAGATATTGA TGAACATGAA 
AAAAGATCAA AGCGAGTGTC ATCTTTAAAT AGTTCTACTC ATAAATCAAG TGACAATAAA 
GTAGAAGAGA CCCTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT CCCTATTTCT 
GAAGAGACTA TAAAACTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC AATTCAAGTT 
AAGACTTTTG GTCCTGTATA CGAAGGAAAA GATTTAATTG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATTCCCTTG ATTGAAAGAC TCCAAAGAAA TCAAGAGACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CTACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATGTAACT AGGAAACTCA GTGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGG 
ACCCCAGGTC GTATCAAAGA CCATCTGCAG AGCGGCCGCT TAGATCTTTC TAAACTGCGC 
CACGTTGTGC TTGATGAAGT GGATCAAATG TTAGATTTAG GTTTTGCTGA ACAAGTTGAA 
GACATTCTCC ATGAGTCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTGCTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATATATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG GAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GCCACTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATCTTC TGTGAGACCA AAAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT CGCTCAGTCA 
CAAAGAGAAA TTACACTGAA AGGCTTCAGA GAGGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAGGTTGACC TGGTGATTCA GAGTTCACCT 
CCGCAGGATG TTGAGTCCTA CATTCATCGC TCTGGACGCA CAGGTCGAGC TGGCCGGACA 
GGGATCTGCA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AATTACGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTTTGGCT TCTGTTTCTT ATGCTGCTGT CGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAGGGGGCAG TGGATGCGCT GGCTGCAGCG 
TTAGCCCACA TCTCTGGCGC GTCAAGTTTT GAACCACGAT CTTTGATCAC CTCCGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCCA GAAGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCTGTGTCCC AGATTACCAG AATGTGCCTC 
CTAAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCCACAA CTGAATCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCAGA CTGGATACTC TCAGTGCCAG CCAAGTTGCC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGTTG GTCAAGTGGC 
CGGTTGGGCC GGTCAGGCCG GTCAGGTGGT CGATCCGGTG GCCGGTCAGG TAGACAGAGT 
CGGCAGGGGA GTCGGACAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAGCGGAAT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX50 ( XM_010820595.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818897.1 Bos taurus DExD-box helicase 50 (DDX50), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa231618
Clone ID Related Accession (Same CDS sequence)	XM_024986700.1 , XM_010820595.3 , XM_005226430.4
Accession Version	XM_010820595.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ATP-dependent RNA helicase DDX50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820595.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGCTAAACGG TGACACCGAG GAAGGATGTG ATAGACTTTC AGATGAATTT 
TCTAAATCTC ATAAGCCAAG AAGAAAAGAT CTATCAAATG GAGATATTGA TGAACATGAA 
AAAAGATCAA AGCGAGTGTC ATCTTTAAAT AGTTCTACTC ATAAATCAAG TGACAATAAA 
GTAGAAGAGA CCCTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT CCCTATTTCT 
GAAGAGACTA TAAAACTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC AATTCAAGTT 
AAGACTTTTG GTCCTGTATA CGAAGGAAAA GATTTAATTG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATTCCCTTG ATTGAAAGAC TCCAAAGAAA TCAAGAGACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CTACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATGTAACT AGGAAACTCA GTGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGG 
ACCCCAGGTC GTATCAAAGA CCATCTGCAG AGCGGCCGCT TAGATCTTTC TAAACTGCGC 
CACGTTGTGC TTGATGAAGT GGATCAAATG TTAGATTTAG GTTTTGCTGA ACAAGTTGAA 
GACATTCTCC ATGAGTCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTGCTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATATATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG GAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GCCACTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATCTTC TGTGAGACCA AAAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT CGCTCAGTCA 
CAAAGAGAAA TTACACTGAA AGGCTTCAGA GAGGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAGGTTGACC TGGTGATTCA GAGTTCACCT 
CCGCAGGATG TTGAGTCCTA CATTCATCGC TCTGGACGCA CAGGTCGAGC TGGCCGGACA 
GGGATCTGCA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AATTACGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTTTGGCT TCTGTTTCTT ATGCTGCTGT CGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAGGGGGCAG TGGATGCGCT GGCTGCAGCG 
TTAGCCCACA TCTCTGGCGC GTCAAGTTTT GAACCACGAT CTTTGATCAC CTCCGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCCA GAAGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCTGTGTCCC AGATTACCAG AATGTGCCTC 
CTAAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCCACAA CTGAATCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCAGA CTGGATACTC TCAGTGCCAG CCAAGTTGCC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGTTG GTCAAGTGGC 
CGGTTGGGCC GGTCAGGCCG GTCAGGTGGT CGATCCGGTG GCCGGTCAGG TAGACAGAGT 
CGGCAGGGGA GTCGGACAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAGCGGAAT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818897.1
CDS	1484..3502
Translation	MKEKLNGDTEEGCDRLSDEFSKSHKPRRKDLSNGDIDEHEKRSKRVSSLNSSTHKSSDNKVEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDVTRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDILHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESPEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRLGRSGRSGGRSGGRSGRQSRQGSRTGSRQDGRRRSGNRNRSRSGGHKRNFD

Target ORF information:

RefSeq Version	XM_010820595.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DExD-box helicase 50 (DDX50), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820595.3

ORF Insert Sequence:

ATGAAAGAGA AGCTAAACGG TGACACCGAG GAAGGATGTG ATAGACTTTC AGATGAATTT 
TCTAAATCTC ATAAGCCAAG AAGAAAAGAT CTATCAAATG GAGATATTGA TGAACATGAA 
AAAAGATCAA AGCGAGTGTC ATCTTTAAAT AGTTCTACTC ATAAATCAAG TGACAATAAA 
GTAGAAGAGA CCCTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT CCCTATTTCT 
GAAGAGACTA TAAAACTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC AATTCAAGTT 
AAGACTTTTG GTCCTGTATA CGAAGGAAAA GATTTAATTG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATTCCCTTG ATTGAAAGAC TCCAAAGAAA TCAAGAGACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CTACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATGTAACT AGGAAACTCA GTGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGG 
ACCCCAGGTC GTATCAAAGA CCATCTGCAG AGCGGCCGCT TAGATCTTTC TAAACTGCGC 
CACGTTGTGC TTGATGAAGT GGATCAAATG TTAGATTTAG GTTTTGCTGA ACAAGTTGAA 
GACATTCTCC ATGAGTCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTGCTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATATATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG GAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GCCACTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATCTTC TGTGAGACCA AAAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT CGCTCAGTCA 
CAAAGAGAAA TTACACTGAA AGGCTTCAGA GAGGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAGGTTGACC TGGTGATTCA GAGTTCACCT 
CCGCAGGATG TTGAGTCCTA CATTCATCGC TCTGGACGCA CAGGTCGAGC TGGCCGGACA 
GGGATCTGCA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AATTACGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTTTGGCT TCTGTTTCTT ATGCTGCTGT CGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAGGGGGCAG TGGATGCGCT GGCTGCAGCG 
TTAGCCCACA TCTCTGGCGC GTCAAGTTTT GAACCACGAT CTTTGATCAC CTCCGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCCA GAAGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCTGTGTCCC AGATTACCAG AATGTGCCTC 
CTAAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCCACAA CTGAATCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCAGA CTGGATACTC TCAGTGCCAG CCAAGTTGCC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGTTG GTCAAGTGGC 
CGGTTGGGCC GGTCAGGCCG GTCAGGTGGT CGATCCGGTG GCCGGTCAGG TAGACAGAGT 
CGGCAGGGGA GTCGGACAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAGCGGAAT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX50 ( XM_005226430.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226487.1 Bos taurus DExD-box helicase 50 (DDX50), transcript variant X1, mRNA.

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CloneID	OBa231618
Clone ID Related Accession (Same CDS sequence)	XM_024986700.1 , XM_010820595.3 , XM_005226430.4
Accession Version	XM_005226430.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ATP-dependent RNA helicase DDX50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226430.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGCTAAACGG TGACACCGAG GAAGGATGTG ATAGACTTTC AGATGAATTT 
TCTAAATCTC ATAAGCCAAG AAGAAAAGAT CTATCAAATG GAGATATTGA TGAACATGAA 
AAAAGATCAA AGCGAGTGTC ATCTTTAAAT AGTTCTACTC ATAAATCAAG TGACAATAAA 
GTAGAAGAGA CCCTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT CCCTATTTCT 
GAAGAGACTA TAAAACTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC AATTCAAGTT 
AAGACTTTTG GTCCTGTATA CGAAGGAAAA GATTTAATTG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATTCCCTTG ATTGAAAGAC TCCAAAGAAA TCAAGAGACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CTACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATGTAACT AGGAAACTCA GTGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGG 
ACCCCAGGTC GTATCAAAGA CCATCTGCAG AGCGGCCGCT TAGATCTTTC TAAACTGCGC 
CACGTTGTGC TTGATGAAGT GGATCAAATG TTAGATTTAG GTTTTGCTGA ACAAGTTGAA 
GACATTCTCC ATGAGTCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTGCTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATATATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG GAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GCCACTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATCTTC TGTGAGACCA AAAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT CGCTCAGTCA 
CAAAGAGAAA TTACACTGAA AGGCTTCAGA GAGGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAGGTTGACC TGGTGATTCA GAGTTCACCT 
CCGCAGGATG TTGAGTCCTA CATTCATCGC TCTGGACGCA CAGGTCGAGC TGGCCGGACA 
GGGATCTGCA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AATTACGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTTTGGCT TCTGTTTCTT ATGCTGCTGT CGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAGGGGGCAG TGGATGCGCT GGCTGCAGCG 
TTAGCCCACA TCTCTGGCGC GTCAAGTTTT GAACCACGAT CTTTGATCAC CTCCGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCCA GAAGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCTGTGTCCC AGATTACCAG AATGTGCCTC 
CTAAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCCACAA CTGAATCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCAGA CTGGATACTC TCAGTGCCAG CCAAGTTGCC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGTTG GTCAAGTGGC 
CGGTTGGGCC GGTCAGGCCG GTCAGGTGGT CGATCCGGTG GCCGGTCAGG TAGACAGAGT 
CGGCAGGGGA GTCGGACAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAGCGGAAT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226487.1
CDS	443..2461
Translation	MKEKLNGDTEEGCDRLSDEFSKSHKPRRKDLSNGDIDEHEKRSKRVSSLNSSTHKSSDNKVEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDVTRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDILHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESPEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRLGRSGRSGGRSGGRSGRQSRQGSRTGSRQDGRRRSGNRNRSRSGGHKRNFD

Target ORF information:

RefSeq Version	XM_005226430.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DExD-box helicase 50 (DDX50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226430.4

ORF Insert Sequence:

ATGAAAGAGA AGCTAAACGG TGACACCGAG GAAGGATGTG ATAGACTTTC AGATGAATTT 
TCTAAATCTC ATAAGCCAAG AAGAAAAGAT CTATCAAATG GAGATATTGA TGAACATGAA 
AAAAGATCAA AGCGAGTGTC ATCTTTAAAT AGTTCTACTC ATAAATCAAG TGACAATAAA 
GTAGAAGAGA CCCTAACACG TGAACAGAAA GAAGGAGCCT TCTCCAATTT CCCTATTTCT 
GAAGAGACTA TAAAACTTCT GAAAGGTCGA GGGGTAACAT ATCTCTTTCC AATTCAAGTT 
AAGACTTTTG GTCCTGTATA CGAAGGAAAA GATTTAATTG CTCAAGCACG GACAGGAACA 
GGAAAGACAT TCTCTTTTGC CATTCCCTTG ATTGAAAGAC TCCAAAGAAA TCAAGAGACA 
ATTAAAAAAA GCCGCTCACC AAAGGTACTT GTTTTGGCTC CTACAAGGGA ACTGGCAAAC 
CAAGTAGCCA AAGACTTCAA AGATGTAACT AGGAAACTCA GTGTGGCGTG TTTTTATGGT 
GGAACATCAT ATCAAAGCCA AATTAATCAT ATTCGAAATG GTATTGACAT CTTGGTTGGG 
ACCCCAGGTC GTATCAAAGA CCATCTGCAG AGCGGCCGCT TAGATCTTTC TAAACTGCGC 
CACGTTGTGC TTGATGAAGT GGATCAAATG TTAGATTTAG GTTTTGCTGA ACAAGTTGAA 
GACATTCTCC ATGAGTCCTA CAAAACTGAT TCTGAAGACA ATCCTCAGAC TTTGCTTTTT 
TCTGCAACTT GCCCACAGTG GGTATACAAA GTTGCAAAAA AATATATGAA ATCCAGATAT 
GAACAGGTTG ACCTTGTTGG GAAAATGACT CAAAAGGCTG CAACTACTGT GGAACATTTG 
GCCATCCAGT GCCACTGGTC TCAGAGGCCA GCAGTTATTG GAGATGTCCT TCAAGTCTAC 
AGTGGGTCTG AAGGGAGGGC TATTATCTTC TGTGAGACCA AAAAGAATGT AACTGAAATG 
GCCATGAATC CACACATAAA ACAGAATGCC CAGTGTTTAC ATGGGGACAT CGCTCAGTCA 
CAAAGAGAAA TTACACTGAA AGGCTTCAGA GAGGGTAGTT TTAAAGTTTT GGTGGCAACC 
AATGTGGCTG CCCGTGGTTT GGACATTCCT GAGGTTGACC TGGTGATTCA GAGTTCACCT 
CCGCAGGATG TTGAGTCCTA CATTCATCGC TCTGGACGCA CAGGTCGAGC TGGCCGGACA 
GGGATCTGCA TATGTTTTTA TCAACCAAGA GAAAGAGGTC AATTACGATA TGTGGAACAA 
AAAGCAGGAA TTACTTTTAA ACGTGTAGGT GTTCCTTCTA CAATGGATTT AGTTAAATCT 
AAAAGCATGG ATGCCATCAG GTCTTTGGCT TCTGTTTCTT ATGCTGCTGT CGATTTTTTC 
CGACCATCAG CTCAGAGACT GATAGAAGAG AAGGGGGCAG TGGATGCGCT GGCTGCAGCG 
TTAGCCCACA TCTCTGGCGC GTCAAGTTTT GAACCACGAT CTTTGATCAC CTCCGATAAG 
GGGTTTGTGA CCATGACTCT GGAAAGCCCA GAAGAAATAC AGGATGTCAG CTGTGCTTGG 
AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCTGTGTCCC AGATTACCAG AATGTGCCTC 
CTAAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCCACAA CTGAATCAGA AAGGTTACAG 
GCAGAGTGGC ATGATTCAGA CTGGATACTC TCAGTGCCAG CCAAGTTGCC TGAAATTGAA 
GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA GGAGTGGTTG GTCAAGTGGC 
CGGTTGGGCC GGTCAGGCCG GTCAGGTGGT CGATCCGGTG GCCGGTCAGG TAGACAGAGT 
CGGCAGGGGA GTCGGACAGG AAGTCGACAA GATGGTAGAA GACGAAGTGG GAATAGAAAT 
CGATCAAGAA GTGGGGGCCA CAAGCGGAAT TTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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DDX50 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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