DARS cDNA ORF clone, Pygoscelis adeliae(Adelie penguin)

Gene
Clones
gRNAs

Summary

Gene Symbol	DARS
Entrez Gene ID	103912629
Full Name	aspartyl-tRNA synthetase
Gene Type	protein-coding
Organism	Pygoscelis adeliae(Adelie penguin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009318935.1	XP_009317210.1	aspartate--tRNA ligase, cytoplasmic

Pathways from BioSystems
Gene Ontology

The following DARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPy111081	XM_009318935.1 Latest version!	Pygoscelis adeliae aspartyl-tRNA synthetase (DARS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DARS ( XM_009318935.1 ) cDNA ORF clone, Pygoscelis adeliae(Adelie penguin) -> XP_009317210.1 Pygoscelis adeliae aspartyl-tRNA synthetase (DARS), mRNA.

Price & Availability↑

CloneID	OPy111081
Clone ID Related Accession (Same CDS sequence)	XM_009318935.1
Accession Version	XM_009318935.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-25
Organism	Pygoscelis adeliae(Adelie penguin)
Product	aspartate--tRNA ligase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008824309.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pygoscelis adeliae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATACAAT CCCAAGAGAA ACCAGATAGA GTTTTGATTC GAATAAAAGA CTTGACAGTG 
GAGAAGGCTG ATGAAGTGGT TTGGGTTCGT GGCAGAATTC ATACAAGCAG AGCTAAAGGG 
AAGCAGTGTT TCTTAGTCCT GCGTCAGCAG CAGTTTAATA TCCAGGCTCT TGTGGCTGTG 
GGACAGCATG CAAGCAAGCA GATGGTAAAG TTTGCTGCCA ACATCAACAA AGAGAGCATT 
GTGGATGTAG AAGGCGTTGT GAGGAAAGCA CATCAGAAAA TCGGAGGTTG TACTCAGCAA 
GATGTTGAGC TTCACATTCA AAGGATCTAT GTGATCAGTT TAGCTGAACC CCGACTGCCC 
TTACAGCTGG ATGATGCTGT TCGGCCAGAA GTGGAAGGAG AGGAGGATGG AAGAGCTACT 
GTAAACCAAG ATACAAGACT GGACAACAGA GTCATTGATC TGAGGACCTC CACTAGTCAG 
GCAATCTTCT GCCTTCAGTC TGGTATTTGT CAGCTTTTCC GAGAAACTCT TATTCGCAAG 
GGATTTGTGG AAATTCAGAC CCCCAAAATC ATTTCAGCTG CCAGTGAAGG AGGAGCCAAT 
GTGTTTACTG TATCATACTT CAAAAGCAGT GCCTACCTGG CTCAGTCCCC ACAGCTGTAC 
AAGCAGATGT GTATATGTGC TGACTTTGAA AAGGTTTTTT GTGTTGGGCC AGTATTTAGA 
GCTGAGGACT CCAACACACA CAGACACCTG ACTGAGTTTG TTGGTCTGGA TATTGAAATG 
GCTTTTAACT ATCACTATCA TGAAGTGGTA GATGAGATTG CAGATACCTT GGTGCAAATA 
TTCAAGGGAC TTCAGGAAAG ATTCCAAACT GAAATTCGGA CAGTGAACAA ACAGTTCCCA 
TGTGAGCCGT TTAAGTTTTT GGAGCCAACT CTGAGGCTGG AATACCGTGA AGCTGTGGCC 
ATGCTTAGAG AGGCTGGTGT TGAGATGGGT GATGAAGAAG ATCTGAGCAC ACCTAATGAA 
AAGCTCCTGG GACGCCTGGT AAAGGAGAAG TATGACACAG ACTTCTATAT TCTTGACAAA 
TATCCTCTTG CTGTGAGGCC TTTTTATACA ATGCCAGACC CAGTAAATCC CAAAAACTCT 
AACTCTTACG ATATGTTCAT GAGAGGGGAA GAGATCTTGT CTGGAGCTCA GAGAATTCAC 
GATCCTCAGC TGCTGACAGA AAGAGCCCAG CATCACGGCA TCGATTTGGA GAAAATAAAG 
GCTTATATTG ATTCCTTTCG TTTTGGTGCA CCTCCACACG CAGGTGGTGG AATAGGGCTG 
GAAAGAGTAA CCATGCTGTA CCTAGGGCTG CATAATGTTC GTCAGACCTC CATGTTCCCA 
CGGGATCCCA AACGACTGAC ACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009317210.1
CDS	32..1438
Translation	MIQSQEKPDRVLIRIKDLTVEKADEVVWVRGRIHTSRAKGKQCFLVLRQQQFNIQALVAVGQHASKQMVKFAANINKESIVDVEGVVRKAHQKIGGCTQQDVELHIQRIYVISLAEPRLPLQLDDAVRPEVEGEEDGRATVNQDTRLDNRVIDLRTSTSQAIFCLQSGICQLFRETLIRKGFVEIQTPKIISAASEGGANVFTVSYFKSSAYLAQSPQLYKQMCICADFEKVFCVGPVFRAEDSNTHRHLTEFVGLDIEMAFNYHYHEVVDEIADTLVQIFKGLQERFQTEIRTVNKQFPCEPFKFLEPTLRLEYREAVAMLREAGVEMGDEEDLSTPNEKLLGRLVKEKYDTDFYILDKYPLAVRPFYTMPDPVNPKNSNSYDMFMRGEEILSGAQRIHDPQLLTERAQHHGIDLEKIKAYIDSFRFGAPPHAGGGIGLERVTMLYLGLHNVRQTSMFPRDPKRLTP

Target ORF information:

RefSeq Version	XM_009318935.1
Organism	Pygoscelis adeliae(Adelie penguin)
Definition	Pygoscelis adeliae aspartyl-tRNA synthetase (DARS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009318935.1

ORF Insert Sequence:

ATGATACAAT CCCAAGAGAA ACCAGATAGA GTTTTGATTC GAATAAAAGA CTTGACAGTG 
GAGAAGGCTG ATGAAGTGGT TTGGGTTCGT GGCAGAATTC ATACAAGCAG AGCTAAAGGG 
AAGCAGTGTT TCTTAGTCCT GCGTCAGCAG CAGTTTAATA TCCAGGCTCT TGTGGCTGTG 
GGACAGCATG CAAGCAAGCA GATGGTAAAG TTTGCTGCCA ACATCAACAA AGAGAGCATT 
GTGGATGTAG AAGGCGTTGT GAGGAAAGCA CATCAGAAAA TCGGAGGTTG TACTCAGCAA 
GATGTTGAGC TTCACATTCA AAGGATCTAT GTGATCAGTT TAGCTGAACC CCGACTGCCC 
TTACAGCTGG ATGATGCTGT TCGGCCAGAA GTGGAAGGAG AGGAGGATGG AAGAGCTACT 
GTAAACCAAG ATACAAGACT GGACAACAGA GTCATTGATC TGAGGACCTC CACTAGTCAG 
GCAATCTTCT GCCTTCAGTC TGGTATTTGT CAGCTTTTCC GAGAAACTCT TATTCGCAAG 
GGATTTGTGG AAATTCAGAC CCCCAAAATC ATTTCAGCTG CCAGTGAAGG AGGAGCCAAT 
GTGTTTACTG TATCATACTT CAAAAGCAGT GCCTACCTGG CTCAGTCCCC ACAGCTGTAC 
AAGCAGATGT GTATATGTGC TGACTTTGAA AAGGTTTTTT GTGTTGGGCC AGTATTTAGA 
GCTGAGGACT CCAACACACA CAGACACCTG ACTGAGTTTG TTGGTCTGGA TATTGAAATG 
GCTTTTAACT ATCACTATCA TGAAGTGGTA GATGAGATTG CAGATACCTT GGTGCAAATA 
TTCAAGGGAC TTCAGGAAAG ATTCCAAACT GAAATTCGGA CAGTGAACAA ACAGTTCCCA 
TGTGAGCCGT TTAAGTTTTT GGAGCCAACT CTGAGGCTGG AATACCGTGA AGCTGTGGCC 
ATGCTTAGAG AGGCTGGTGT TGAGATGGGT GATGAAGAAG ATCTGAGCAC ACCTAATGAA 
AAGCTCCTGG GACGCCTGGT AAAGGAGAAG TATGACACAG ACTTCTATAT TCTTGACAAA 
TATCCTCTTG CTGTGAGGCC TTTTTATACA ATGCCAGACC CAGTAAATCC CAAAAACTCT 
AACTCTTACG ATATGTTCAT GAGAGGGGAA GAGATCTTGT CTGGAGCTCA GAGAATTCAC 
GATCCTCAGC TGCTGACAGA AAGAGCCCAG CATCACGGCA TCGATTTGGA GAAAATAAAG 
GCTTATATTG ATTCCTTTCG TTTTGGTGCA CCTCCACACG CAGGTGGTGG AATAGGGCTG 
GAAAGAGTAA CCATGCTGTA CCTAGGGCTG CATAATGTTC GTCAGACCTC CATGTTCCCA 
CGGGATCCCA AACGACTGAC ACCCTAA