LOC427439 cDNA ORF clone, Gallus gallus(chicken)

The following LOC427439 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC427439 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa47845 XM_015277524.2
Latest version!
Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa20949 XM_004937195.3
Latest version!
Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OGa20946 XM_425012.6
Latest version!
Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa20946 XM_015277519.2
Latest version!
Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa20946 XM_015277520.2
Latest version!
Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa20947 XM_004937193.3
Latest version!
Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa20948 XM_015277521.2
Latest version!
Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
View Old Accession Versions >>
OGa20946 XM_425012.5 Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa20946 XM_015277519.1 Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa47845 XM_015277524.1 Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa20949 XM_004937195.2 Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OGa20947 XM_004937193.2 Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa20946 XM_015277520.1 Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa20948 XM_015277521.1 Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa47845
    Clone ID Related Accession (Same CDS sequence) XM_015277524.1 , XM_015277524.2
    Accession Version XM_015277524.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product nipped-B-like protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG TAGCATAGAT CAGTCAGTGC TAAAGGAGTT GCCTCCTGAG
    CTCCTGGCAG AAATTGAATC CACCATGCCT CTTTGTGAAC GCGTGAAAAT GAACAAGCGC
    AAACGTAGCA CAGTTAATGA GAAACCAAAA TATGCTGAAA TCAGTTCTGA TGAAGACAAT
    GACAGTGAAG AAGCTTTTGA ATCTTCTCGA AAAAGACATA AAAAGGATAG AGATGAAGCT
    TGGGAGTACG AAGAGCATGA CAGAAGAAGT TCTGGTGACC ACAGGAGGAG TGGTCATTAT
    CATGAAGGAA GAAGGACTTC TGGTAGTGGC CGTTACCGCA ATCGCAGTCC CGATGACTCT
    GATCTGGATG ATTATTCTCC TCCTCCTAGC CTCAGTGAGG TGGCAAGAAA AATGAAGAAA
    AAAGAAAAGC AAAAGAAGAG GAGAGCTTAC GAGCCTAAAC TAACACCTGA AGAAATGATG
    GATTCTTCAA CCTTCAAAAG ATTTATTGCT TCAATAGAGA ATATTCTGGA AAATCTGGAA
    GACATGGATT TTACAACCTT GGGTGATGAT GATGAGATTC CACAGGAATT GCTTTTGGGA
    AAACATCAGC TTAATGAGTT GGGTAGTGAA TCTGCAAAAA TCAAGGCAAT GGGTATTATG
    GACAAGCTCT CAACAGATAA AATAGTAAAG GTCCTGAACA TTTTGGAGAA GAATATTCAA
    GACGGATCAA AGCTTTCTAC ATTACTTAAC CACAACAATG ACACTGAAGA TGAGGAGAGA
    TTATGGAGAG ATCTCATAAT GGAGAGAGTA ACAAAATCTG CAGATGCTTG TCTTACTGCT
    ATCAATATTA TGACATCACC AAATATGCCT AAAGCTGTTT ATATTGAGGA CGTAATAGAA
    AGAGTTATTC AGTACACAAA ATTTCATTTG CAGAACACTC TCTATCCTCA GTATGACCCT
    GTGTATAGAG TGGATCCTCT TGGTGGTGGT CTTTTAAGTT CAAAAGCAAA ACGTGCCAAG
    TGTTCCACCC ACAAACAAAG AGTTATAGTG ATGTTGTATA ACAAAGTTTG TGATATTGTC
    AGCAGTTTGT CAGAGTTGCT AGAGATACAA CTTCTTACTG ATACAACTAT TCTTCAGGTA
    TCGTCTATGG GGATAACACC TTTCTTTGTA GAAAATGTCA GTGAACTACA GCTATGTGCC
    ATCAAATTAG TGACTGCAGT CTTCTCCAGG TATGAAAAAC ATAGACAGCT AATACTAGAA
    GAAATTTTTA CTTCCCTTGC AAGACTACCA ACTAGCAAGA GGAGCCTGAG AAACTTCAGG
    TTGAATAGCA GTGACACAGA TGGAGAGCCT ATGTATATTC AGATGGTAAC AGCACTAGTT
    CTGCAGCTTA TTCAATGTGT CGTGCACTTA CCATCAGCAG AAAAAGATTC TAATTCAGAT
    GAGGAATTGA ACAAAAAAGT GGATCAAGAT GTGCTTATTA CTAACTCATA TGAAACAGCT
    ATGAGAACAG CACAAAATTT TCTTTCTATT TTTCTTAAGA AATGTGGTAG TAAACAAGGA
    GAAGAAGATT ACAGGCCTCT CTTTGAGAAC TTCATTCAAG ATCTTCTTTC CACAGTCAAT
    AAACCAGAAT GGCCAGCTGC AGAGTTGCTG CTTAGCTTAC TAGGAAGGTT ATTGGTTCAC
    CAGTTCAGTA ATAAATCAAC AGAAATGGCT TTAAGAGTTG CGTCACTTGA CTATCTTGGA
    ACCGTAGCTG CAAGACTGAG GAAAGATGCA GTCACTAGTA AAATGGATCA AGGATCTATA
    GCTAGAATCC TCAAACAGGC CTCAGGAGGA GAAGATGATA TTCAACAGCT ACAGAAGGCT
    TTATTGGATT ATCTGGATGA GAACACTGAA ACTGATGCAT CGTTAGTGTT TTCTCGGAAG
    TTTTATATAG CTCAGTGGTT TCGGGATACA ACTATGGAGA CAGAGAAGGC AATTAAATCT
    CAGAAGGATG AGGATTCATC TGAAGGAACT CATCATGCAA AAGATGTTGA GACCACAGGA
    CAAATCATGC ACAGAGCAGA AAGCCGCAAG AAGTTTCTCC GCAGCATCAT TAAAACTGCT
    CCATCTCAGT TCAGTACATT AAAGATTTAT TCTGACACTG TGGACTATGA AGATGCATGT
    TTGATTGTTC GCTACTTGGC CTCTATGAGG CCGTTTGCCC AGAGCTTCGA TATTTATTTA
    ACACAGATTC TGCGTGTACT TGGTGAAAAT GCAATTGCAG TTCGAACAAA AGCCATGAAG
    TGTTTGTCTG AGGTTGTTGC TGTAGATCCC AGTATTCTAG CCAGGCCGGA TATGCAGCGA
    GGTGTTCATG GACGGTTAAT GGATAACTCC ACCAGTGTAC GGGAAGCAGC TGTGGAGCTG
    CTTGGCCGAT TTGTGCTCTG TCGTCCTCAG CTTGCTGAGC AGTATTATGA CATGCTGATT
    GAAAGAATAT TGGATACTGG TATCAGTGTC AGAAAAAGAG TCATAAAGAT ACTTAGGGAT
    ATCTGCATTG AACAACCAAC GTTTCCCAAA ATTACAGAGA TGTGTGTGAA AATGATTCGG
    AGAGTCAATG ATGAAGAAGG CATTAAGAAA TTAGTAAATG AGACATTCCA GAAGCTGTGG
    TTTACTCCAA CTCCACACCA TGACAAAGAA GCAATGACTA GGAAAATACT GAACATCACT
    GATGTGGTGG CAGCTTGTAG GGATACAGGG TATGATTGGT TTGAGCAGCT TCTTCAAAAT
    CTATTGAAGT CAGAAGAGGA TGCTTCATAT AAACCTGTGA AGAAGGCTTG TACTCAACTT
    GTTGACAATT TGGTTGAGCA CATCCTTAAA TATGAGGAGT CTTTATCAGA CTCTGACAAC
    AAAGGAGTGA ATTCTGGTCG CTTGGTTGCT TGTATAACCA CTCTGTTCTT ATTCAGCAAA
    ATCAGGCCCC AGTTAATGGT CAAACATGCC ATGACCATGC AGCCATATCT GGCAACTAAA
    TGTAGTACTC AGAATGATTT CATGGTGATC TGCAATGTTG CAAAAATCTT GGAGCTTGTA
    GTGCCGTTAA TGGAACACCC AAGTGAGACC TTCCTTGCCA CTATTGAGGA AGACCTCATG
    AAACTCATCA TCAAATATGG CATGACAGTT GTTCAACATT GTGTGAGCTG CCTTGGGTCT
    GTTGTGAACA AGGTCACCCA GAATTACAAG TTTGTGTGGG CCTGTTTTAA TAGATACTAT
    GGTGCACTTT CAAAATTAAA AAGTCAACAC CAAGAAGACC CCAACAGTAC AATACTTACT
    GCCAATAAAC CAGCACTCCT TAGATCACTT TTCACTGTTG GAGCACTGTG TCGGCATTTT
    GATTTTGACC ATGAAGATTT TAAGGGCAAC AGTAAGGTTA ACATTAAGGA TAAAGTACTT
    GAACTGCTGA TGTATTTTAC AAAGCATTCA GACGAAGAAG TACAGACAAA AGCCATTATT
    GGCCTGGGAT TTGCATTTAT ACAACACCCA AGCTTAATGT TTGAACAGGA AGTAAAGACT
    CTGTACAACA GCATTCTTTC AGATAAGAAC TGTTCAGTAA ACCTAAAAAT CCAGGTGTTA
    AAAAACCTCC AGACCTACTT GCAGGAGGAG GACACACGTA TGCAGCAGGC AGACAGAGAC
    TGGAAAAAAG TAGCAAAACA GGAAGACCTG AAAGAAATGG GTGATATTTC CTCAGGGATG
    AGTAGTTCCA TCATGCAGCT GTATCTCAAA CAGGTCCTTG AGGCTTTCTT TCATACCCAG
    TCCAATGTAC GCCACTTTGC TCTAAATGTC ATTGCACTGA CGTTAAACCA GGGTCTCATC
    CATCCTGTTC AGTGTGTGCC ATACTTAATT GCTATGGGCA CAGATCCAGA GCCTTCTATG
    CGAAACAAAG CTGACCAGCA GTTGGTGGAA ATAGACAAAA AGTATGCTGG TTTCATTCAC
    ATGAAAGCAG TGGCTGGTAT GAAGATGTCT TACCAGGTAC AACAGGCAAT CAATACCTGC
    CCAAAGGATC CTGTAAGAGG CTTTAGACAC GATGAATCAT CCAGTGCATT GTGTTCACAC
    CTATATTCTA TGATCCGAGG GAACCGTCAA CACAGACGAG CATTTCTAAT TTCTTTACTC
    AATCTCTTCG ATGATACTGC AAAAACAGAG GTGAATATGC TCTTGTACAT AGCAGACAAT
    CTAGCCTGTT TTCCTTATCA AACGCAGGAA GAGCCACTGT TTATAATGCA TCATATAGAC
    ATTACACTAT CAGTTTCTGG TAGCAACCTA CTACAGTCAT TTAAAGAGTC AATGGTGAAA
    GACAAAAAAA AGGAAAGAAA GCCTTCATCA GATGAGGAGA CTGAGAGTGA CAGTAATAGT
    GGCAGTGAGA GCGAAAGTGA AGAGGAAGCT TCTAAGCCTC GGAGGTTACG AAAACGAGTA
    GATTCTGACT CAGATTCTGA TGAAGAAAAT GATATAAATG CTGTGATGAA ATGTTTACCA
    GAAAACTCAG CTCCTTTAAT TGAATTTGCA AATGTGTCTC AAGGCATATT ATTACTTTTG
    ATGCTGAAAC AGCATTTAAA GAACCTTTGC GGATTCTCTG ATAGTAAAAT TCAGAAATAT
    TCTCCATCTG AGTCTGCAAA AGTTTATGAT AAAGCGATAA ACAGGAAGAC AGGAGTGCAT
    TTCCATCCAA AACAAACTCT GGATTTTCTA CGGAGTGATA TGGCTAATTC CAAGATCACT
    GAAGAAGTGA AGAGGAGTAT AGTAAAACAG TACTTAGATT TCAAGCTCCT TATGGAACAT
    TTGGATCCTG ATGAAGAAGA AGAAGATGGA GAGGTGTCAG CTAGCACAAA TGCTCGGAAC
    AAAGCAATTA CCTCGCTGCT TGGAGGAGGC AGCCCTAAAA ACAATGCAGC TGAGACAGAG
    GACGATGAAA GTGATGGGGA GGATAGAGGA GGAGGCACTT CAGGGTCATT GAGAAGGTCA
    AAACGAAATT CAGACTCTAC GGAGTTGGCT TCACAGATGA ATGAAAGTGT TGATGTCATG
    GATGTCATCG CTATTTGCTG TCCAAAGTAC AAAGATCGAC CACAAATTGC AAGAGTAGTA
    CAGAAAACCA GCAATGGCTT CAGTGTTCAG TGGATGGCAG GCTCCTACAG TGGCTCCTGG
    ACTGAGGCTA AGCGCCGTGA TGGCCGCAAA CTGGTGCCTT GGGTAGACAC TATTAAAGAG
    TCAGACATTA TTTACAAAAA AATTGCTCTA ACGAGTGCTA ATAAGCTGAC TAATAAAGTT
    GTTCAGACTT TACGATCGCT GTATGCCGCC AAGGATGGAA CTTCGAGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133010.1
    CDS3239..9409
    Translation

    Target ORF information:

    RefSeq Version XM_015277524.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG TAGCATAGAT CAGTCAGTGC TAAAGGAGTT GCCTCCTGAG
    CTCCTGGCAG AAATTGAATC CACCATGCCT CTTTGTGAAC GCGTGAAAAT GAACAAGCGC
    AAACGTAGCA CAGTTAATGA GAAACCAAAA TATGCTGAAA TCAGTTCTGA TGAAGACAAT
    GACAGTGAAG AAGCTTTTGA ATCTTCTCGA AAAAGACATA AAAAGGATAG AGATGAAGCT
    TGGGAGTACG AAGAGCATGA CAGAAGAAGT TCTGGTGACC ACAGGAGGAG TGGTCATTAT
    CATGAAGGAA GAAGGACTTC TGGTAGTGGC CGTTACCGCA ATCGCAGTCC CGATGACTCT
    GATCTGGATG ATTATTCTCC TCCTCCTAGC CTCAGTGAGG TGGCAAGAAA AATGAAGAAA
    AAAGAAAAGC AAAAGAAGAG GAGAGCTTAC GAGCCTAAAC TAACACCTGA AGAAATGATG
    GATTCTTCAA CCTTCAAAAG ATTTATTGCT TCAATAGAGA ATATTCTGGA AAATCTGGAA
    GACATGGATT TTACAACCTT GGGTGATGAT GATGAGATTC CACAGGAATT GCTTTTGGGA
    AAACATCAGC TTAATGAGTT GGGTAGTGAA TCTGCAAAAA TCAAGGCAAT GGGTATTATG
    GACAAGCTCT CAACAGATAA AATAGTAAAG GTCCTGAACA TTTTGGAGAA GAATATTCAA
    GACGGATCAA AGCTTTCTAC ATTACTTAAC CACAACAATG ACACTGAAGA TGAGGAGAGA
    TTATGGAGAG ATCTCATAAT GGAGAGAGTA ACAAAATCTG CAGATGCTTG TCTTACTGCT
    ATCAATATTA TGACATCACC AAATATGCCT AAAGCTGTTT ATATTGAGGA CGTAATAGAA
    AGAGTTATTC AGTACACAAA ATTTCATTTG CAGAACACTC TCTATCCTCA GTATGACCCT
    GTGTATAGAG TGGATCCTCT TGGTGGTGGT CTTTTAAGTT CAAAAGCAAA ACGTGCCAAG
    TGTTCCACCC ACAAACAAAG AGTTATAGTG ATGTTGTATA ACAAAGTTTG TGATATTGTC
    AGCAGTTTGT CAGAGTTGCT AGAGATACAA CTTCTTACTG ATACAACTAT TCTTCAGGTA
    TCGTCTATGG GGATAACACC TTTCTTTGTA GAAAATGTCA GTGAACTACA GCTATGTGCC
    ATCAAATTAG TGACTGCAGT CTTCTCCAGG TATGAAAAAC ATAGACAGCT AATACTAGAA
    GAAATTTTTA CTTCCCTTGC AAGACTACCA ACTAGCAAGA GGAGCCTGAG AAACTTCAGG
    TTGAATAGCA GTGACACAGA TGGAGAGCCT ATGTATATTC AGATGGTAAC AGCACTAGTT
    CTGCAGCTTA TTCAATGTGT CGTGCACTTA CCATCAGCAG AAAAAGATTC TAATTCAGAT
    GAGGAATTGA ACAAAAAAGT GGATCAAGAT GTGCTTATTA CTAACTCATA TGAAACAGCT
    ATGAGAACAG CACAAAATTT TCTTTCTATT TTTCTTAAGA AATGTGGTAG TAAACAAGGA
    GAAGAAGATT ACAGGCCTCT CTTTGAGAAC TTCATTCAAG ATCTTCTTTC CACAGTCAAT
    AAACCAGAAT GGCCAGCTGC AGAGTTGCTG CTTAGCTTAC TAGGAAGGTT ATTGGTTCAC
    CAGTTCAGTA ATAAATCAAC AGAAATGGCT TTAAGAGTTG CGTCACTTGA CTATCTTGGA
    ACCGTAGCTG CAAGACTGAG GAAAGATGCA GTCACTAGTA AAATGGATCA AGGATCTATA
    GCTAGAATCC TCAAACAGGC CTCAGGAGGA GAAGATGATA TTCAACAGCT ACAGAAGGCT
    TTATTGGATT ATCTGGATGA GAACACTGAA ACTGATGCAT CGTTAGTGTT TTCTCGGAAG
    TTTTATATAG CTCAGTGGTT TCGGGATACA ACTATGGAGA CAGAGAAGGC AATTAAATCT
    CAGAAGGATG AGGATTCATC TGAAGGAACT CATCATGCAA AAGATGTTGA GACCACAGGA
    CAAATCATGC ACAGAGCAGA AAGCCGCAAG AAGTTTCTCC GCAGCATCAT TAAAACTGCT
    CCATCTCAGT TCAGTACATT AAAGATTTAT TCTGACACTG TGGACTATGA AGATGCATGT
    TTGATTGTTC GCTACTTGGC CTCTATGAGG CCGTTTGCCC AGAGCTTCGA TATTTATTTA
    ACACAGATTC TGCGTGTACT TGGTGAAAAT GCAATTGCAG TTCGAACAAA AGCCATGAAG
    TGTTTGTCTG AGGTTGTTGC TGTAGATCCC AGTATTCTAG CCAGGCCGGA TATGCAGCGA
    GGTGTTCATG GACGGTTAAT GGATAACTCC ACCAGTGTAC GGGAAGCAGC TGTGGAGCTG
    CTTGGCCGAT TTGTGCTCTG TCGTCCTCAG CTTGCTGAGC AGTATTATGA CATGCTGATT
    GAAAGAATAT TGGATACTGG TATCAGTGTC AGAAAAAGAG TCATAAAGAT ACTTAGGGAT
    ATCTGCATTG AACAACCAAC GTTTCCCAAA ATTACAGAGA TGTGTGTGAA AATGATTCGG
    AGAGTCAATG ATGAAGAAGG CATTAAGAAA TTAGTAAATG AGACATTCCA GAAGCTGTGG
    TTTACTCCAA CTCCACACCA TGACAAAGAA GCAATGACTA GGAAAATACT GAACATCACT
    GATGTGGTGG CAGCTTGTAG GGATACAGGG TATGATTGGT TTGAGCAGCT TCTTCAAAAT
    CTATTGAAGT CAGAAGAGGA TGCTTCATAT AAACCTGTGA AGAAGGCTTG TACTCAACTT
    GTTGACAATT TGGTTGAGCA CATCCTTAAA TATGAGGAGT CTTTATCAGA CTCTGACAAC
    AAAGGAGTGA ATTCTGGTCG CTTGGTTGCT TGTATAACCA CTCTGTTCTT ATTCAGCAAA
    ATCAGGCCCC AGTTAATGGT CAAACATGCC ATGACCATGC AGCCATATCT GGCAACTAAA
    TGTAGTACTC AGAATGATTT CATGGTGATC TGCAATGTTG CAAAAATCTT GGAGCTTGTA
    GTGCCGTTAA TGGAACACCC AAGTGAGACC TTCCTTGCCA CTATTGAGGA AGACCTCATG
    AAACTCATCA TCAAATATGG CATGACAGTT GTTCAACATT GTGTGAGCTG CCTTGGGTCT
    GTTGTGAACA AGGTCACCCA GAATTACAAG TTTGTGTGGG CCTGTTTTAA TAGATACTAT
    GGTGCACTTT CAAAATTAAA AAGTCAACAC CAAGAAGACC CCAACAGTAC AATACTTACT
    GCCAATAAAC CAGCACTCCT TAGATCACTT TTCACTGTTG GAGCACTGTG TCGGCATTTT
    GATTTTGACC ATGAAGATTT TAAGGGCAAC AGTAAGGTTA ACATTAAGGA TAAAGTACTT
    GAACTGCTGA TGTATTTTAC AAAGCATTCA GACGAAGAAG TACAGACAAA AGCCATTATT
    GGCCTGGGAT TTGCATTTAT ACAACACCCA AGCTTAATGT TTGAACAGGA AGTAAAGACT
    CTGTACAACA GCATTCTTTC AGATAAGAAC TGTTCAGTAA ACCTAAAAAT CCAGGTGTTA
    AAAAACCTCC AGACCTACTT GCAGGAGGAG GACACACGTA TGCAGCAGGC AGACAGAGAC
    TGGAAAAAAG TAGCAAAACA GGAAGACCTG AAAGAAATGG GTGATATTTC CTCAGGGATG
    AGTAGTTCCA TCATGCAGCT GTATCTCAAA CAGGTCCTTG AGGCTTTCTT TCATACCCAG
    TCCAATGTAC GCCACTTTGC TCTAAATGTC ATTGCACTGA CGTTAAACCA GGGTCTCATC
    CATCCTGTTC AGTGTGTGCC ATACTTAATT GCTATGGGCA CAGATCCAGA GCCTTCTATG
    CGAAACAAAG CTGACCAGCA GTTGGTGGAA ATAGACAAAA AGTATGCTGG TTTCATTCAC
    ATGAAAGCAG TGGCTGGTAT GAAGATGTCT TACCAGGTAC AACAGGCAAT CAATACCTGC
    CCAAAGGATC CTGTAAGAGG CTTTAGACAC GATGAATCAT CCAGTGCATT GTGTTCACAC
    CTATATTCTA TGATCCGAGG GAACCGTCAA CACAGACGAG CATTTCTAAT TTCTTTACTC
    AATCTCTTCG ATGATACTGC AAAAACAGAG GTGAATATGC TCTTGTACAT AGCAGACAAT
    CTAGCCTGTT TTCCTTATCA AACGCAGGAA GAGCCACTGT TTATAATGCA TCATATAGAC
    ATTACACTAT CAGTTTCTGG TAGCAACCTA CTACAGTCAT TTAAAGAGTC AATGGTGAAA
    GACAAAAAAA AGGAAAGAAA GCCTTCATCA GATGAGGAGA CTGAGAGTGA CAGTAATAGT
    GGCAGTGAGA GCGAAAGTGA AGAGGAAGCT TCTAAGCCTC GGAGGTTACG AAAACGAGTA
    GATTCTGACT CAGATTCTGA TGAAGAAAAT GATATAAATG CTGTGATGAA ATGTTTACCA
    GAAAACTCAG CTCCTTTAAT TGAATTTGCA AATGTGTCTC AAGGCATATT ATTACTTTTG
    ATGCTGAAAC AGCATTTAAA GAACCTTTGC GGATTCTCTG ATAGTAAAAT TCAGAAATAT
    TCTCCATCTG AGTCTGCAAA AGTTTATGAT AAAGCGATAA ACAGGAAGAC AGGAGTGCAT
    TTCCATCCAA AACAAACTCT GGATTTTCTA CGGAGTGATA TGGCTAATTC CAAGATCACT
    GAAGAAGTGA AGAGGAGTAT AGTAAAACAG TACTTAGATT TCAAGCTCCT TATGGAACAT
    TTGGATCCTG ATGAAGAAGA AGAAGATGGA GAGGTGTCAG CTAGCACAAA TGCTCGGAAC
    AAAGCAATTA CCTCGCTGCT TGGAGGAGGC AGCCCTAAAA ACAATGCAGC TGAGACAGAG
    GACGATGAAA GTGATGGGGA GGATAGAGGA GGAGGCACTT CAGGGTCATT GAGAAGGTCA
    AAACGAAATT CAGACTCTAC GGAGTTGGCT TCACAGATGA ATGAAAGTGT TGATGTCATG
    GATGTCATCG CTATTTGCTG TCCAAAGTAC AAAGATCGAC CACAAATTGC AAGAGTAGTA
    CAGAAAACCA GCAATGGCTT CAGTGTTCAG TGGATGGCAG GCTCCTACAG TGGCTCCTGG
    ACTGAGGCTA AGCGCCGTGA TGGCCGCAAA CTGGTGCCTT GGGTAGACAC TATTAAAGAG
    TCAGACATTA TTTACAAAAA AATTGCTCTA ACGAGTGCTA ATAAGCTGAC TAATAAAGTT
    GTTCAGACTT TACGATCGCT GTATGCCGCC AAGGATGGAA CTTCGAGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa47845
    Clone ID Related Accession (Same CDS sequence) XM_015277524.1 , XM_015277524.2
    Accession Version XM_015277524.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product nipped-B-like protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277524.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG TAGCATAGAT CAGTCAGTGC TAAAGGAGTT GCCTCCTGAG
    CTCCTGGCAG AAATTGAATC CACCATGCCT CTTTGTGAAC GCGTGAAAAT GAACAAGCGC
    AAACGTAGCA CAGTTAATGA GAAACCAAAA TATGCTGAAA TCAGTTCTGA TGAAGACAAT
    GACAGTGAAG AAGCTTTTGA ATCTTCTCGA AAAAGACATA AAAAGGATAG AGATGAAGCT
    TGGGAGTACG AAGAGCATGA CAGAAGAAGT TCTGGTGACC ACAGGAGGAG TGGTCATTAT
    CATGAAGGAA GAAGGACTTC TGGTAGTGGC CGTTACCGCA ATCGCAGTCC CGATGACTCT
    GATCTGGATG ATTATTCTCC TCCTCCTAGC CTCAGTGAGG TGGCAAGAAA AATGAAGAAA
    AAAGAAAAGC AAAAGAAGAG GAGAGCTTAC GAGCCTAAAC TAACACCTGA AGAAATGATG
    GATTCTTCAA CCTTCAAAAG ATTTATTGCT TCAATAGAGA ATATTCTGGA AAATCTGGAA
    GACATGGATT TTACAACCTT GGGTGATGAT GATGAGATTC CACAGGAATT GCTTTTGGGA
    AAACATCAGC TTAATGAGTT GGGTAGTGAA TCTGCAAAAA TCAAGGCAAT GGGTATTATG
    GACAAGCTCT CAACAGATAA AATAGTAAAG GTCCTGAACA TTTTGGAGAA GAATATTCAA
    GACGGATCAA AGCTTTCTAC ATTACTTAAC CACAACAATG ACACTGAAGA TGAGGAGAGA
    TTATGGAGAG ATCTCATAAT GGAGAGAGTA ACAAAATCTG CAGATGCTTG TCTTACTGCT
    ATCAATATTA TGACATCACC AAATATGCCT AAAGCTGTTT ATATTGAGGA CGTAATAGAA
    AGAGTTATTC AGTACACAAA ATTTCATTTG CAGAACACTC TCTATCCTCA GTATGACCCT
    GTGTATAGAG TGGATCCTCT TGGTGGTGGT CTTTTAAGTT CAAAAGCAAA ACGTGCCAAG
    TGTTCCACCC ACAAACAAAG AGTTATAGTG ATGTTGTATA ACAAAGTTTG TGATATTGTC
    AGCAGTTTGT CAGAGTTGCT AGAGATACAA CTTCTTACTG ATACAACTAT TCTTCAGGTA
    TCGTCTATGG GGATAACACC TTTCTTTGTA GAAAATGTCA GTGAACTACA GCTATGTGCC
    ATCAAATTAG TGACTGCAGT CTTCTCCAGG TATGAAAAAC ATAGACAGCT AATACTAGAA
    GAAATTTTTA CTTCCCTTGC AAGACTACCA ACTAGCAAGA GGAGCCTGAG AAACTTCAGG
    TTGAATAGCA GTGACACAGA TGGAGAGCCT ATGTATATTC AGATGGTAAC AGCACTAGTT
    CTGCAGCTTA TTCAATGTGT CGTGCACTTA CCATCAGCAG AAAAAGATTC TAATTCAGAT
    GAGGAATTGA ACAAAAAAGT GGATCAAGAT GTGCTTATTA CTAACTCATA TGAAACAGCT
    ATGAGAACAG CACAAAATTT TCTTTCTATT TTTCTTAAGA AATGTGGTAG TAAACAAGGA
    GAAGAAGATT ACAGGCCTCT CTTTGAGAAC TTCATTCAAG ATCTTCTTTC CACAGTCAAT
    AAACCAGAAT GGCCAGCTGC AGAGTTGCTG CTTAGCTTAC TAGGAAGGTT ATTGGTTCAC
    CAGTTCAGTA ATAAATCAAC AGAAATGGCT TTAAGAGTTG CGTCACTTGA CTATCTTGGA
    ACCGTAGCTG CAAGACTGAG GAAAGATGCA GTCACTAGTA AAATGGATCA AGGATCTATA
    GCTAGAATCC TCAAACAGGC CTCAGGAGGA GAAGATGATA TTCAACAGCT ACAGAAGGCT
    TTATTGGATT ATCTGGATGA GAACACTGAA ACTGATGCAT CGTTAGTGTT TTCTCGGAAG
    TTTTATATAG CTCAGTGGTT TCGGGATACA ACTATGGAGA CAGAGAAGGC AATTAAATCT
    CAGAAGGATG AGGATTCATC TGAAGGAACT CATCATGCAA AAGATGTTGA GACCACAGGA
    CAAATCATGC ACAGAGCAGA AAGCCGCAAG AAGTTTCTCC GCAGCATCAT TAAAACTGCT
    CCATCTCAGT TCAGTACATT AAAGATTTAT TCTGACACTG TGGACTATGA AGATGCATGT
    TTGATTGTTC GCTACTTGGC CTCTATGAGG CCGTTTGCCC AGAGCTTCGA TATTTATTTA
    ACACAGATTC TGCGTGTACT TGGTGAAAAT GCAATTGCAG TTCGAACAAA AGCCATGAAG
    TGTTTGTCTG AGGTTGTTGC TGTAGATCCC AGTATTCTAG CCAGGCCGGA TATGCAGCGA
    GGTGTTCATG GACGGTTAAT GGATAACTCC ACCAGTGTAC GGGAAGCAGC TGTGGAGCTG
    CTTGGCCGAT TTGTGCTCTG TCGTCCTCAG CTTGCTGAGC AGTATTATGA CATGCTGATT
    GAAAGAATAT TGGATACTGG TATCAGTGTC AGAAAAAGAG TCATAAAGAT ACTTAGGGAT
    ATCTGCATTG AACAACCAAC GTTTCCCAAA ATTACAGAGA TGTGTGTGAA AATGATTCGG
    AGAGTCAATG ATGAAGAAGG CATTAAGAAA TTAGTAAATG AGACATTCCA GAAGCTGTGG
    TTTACTCCAA CTCCACACCA TGACAAAGAA GCAATGACTA GGAAAATACT GAACATCACT
    GATGTGGTGG CAGCTTGTAG GGATACAGGG TATGATTGGT TTGAGCAGCT TCTTCAAAAT
    CTATTGAAGT CAGAAGAGGA TGCTTCATAT AAACCTGTGA AGAAGGCTTG TACTCAACTT
    GTTGACAATT TGGTTGAGCA CATCCTTAAA TATGAGGAGT CTTTATCAGA CTCTGACAAC
    AAAGGAGTGA ATTCTGGTCG CTTGGTTGCT TGTATAACCA CTCTGTTCTT ATTCAGCAAA
    ATCAGGCCCC AGTTAATGGT CAAACATGCC ATGACCATGC AGCCATATCT GGCAACTAAA
    TGTAGTACTC AGAATGATTT CATGGTGATC TGCAATGTTG CAAAAATCTT GGAGCTTGTA
    GTGCCGTTAA TGGAACACCC AAGTGAGACC TTCCTTGCCA CTATTGAGGA AGACCTCATG
    AAACTCATCA TCAAATATGG CATGACAGTT GTTCAACATT GTGTGAGCTG CCTTGGGTCT
    GTTGTGAACA AGGTCACCCA GAATTACAAG TTTGTGTGGG CCTGTTTTAA TAGATACTAT
    GGTGCACTTT CAAAATTAAA AAGTCAACAC CAAGAAGACC CCAACAGTAC AATACTTACT
    GCCAATAAAC CAGCACTCCT TAGATCACTT TTCACTGTTG GAGCACTGTG TCGGCATTTT
    GATTTTGACC ATGAAGATTT TAAGGGCAAC AGTAAGGTTA ACATTAAGGA TAAAGTACTT
    GAACTGCTGA TGTATTTTAC AAAGCATTCA GACGAAGAAG TACAGACAAA AGCCATTATT
    GGCCTGGGAT TTGCATTTAT ACAACACCCA AGCTTAATGT TTGAACAGGA AGTAAAGACT
    CTGTACAACA GCATTCTTTC AGATAAGAAC TGTTCAGTAA ACCTAAAAAT CCAGGTGTTA
    AAAAACCTCC AGACCTACTT GCAGGAGGAG GACACACGTA TGCAGCAGGC AGACAGAGAC
    TGGAAAAAAG TAGCAAAACA GGAAGACCTG AAAGAAATGG GTGATATTTC CTCAGGGATG
    AGTAGTTCCA TCATGCAGCT GTATCTCAAA CAGGTCCTTG AGGCTTTCTT TCATACCCAG
    TCCAATGTAC GCCACTTTGC TCTAAATGTC ATTGCACTGA CGTTAAACCA GGGTCTCATC
    CATCCTGTTC AGTGTGTGCC ATACTTAATT GCTATGGGCA CAGATCCAGA GCCTTCTATG
    CGAAACAAAG CTGACCAGCA GTTGGTGGAA ATAGACAAAA AGTATGCTGG TTTCATTCAC
    ATGAAAGCAG TGGCTGGTAT GAAGATGTCT TACCAGGTAC AACAGGCAAT CAATACCTGC
    CCAAAGGATC CTGTAAGAGG CTTTAGACAC GATGAATCAT CCAGTGCATT GTGTTCACAC
    CTATATTCTA TGATCCGAGG GAACCGTCAA CACAGACGAG CATTTCTAAT TTCTTTACTC
    AATCTCTTCG ATGATACTGC AAAAACAGAG GTGAATATGC TCTTGTACAT AGCAGACAAT
    CTAGCCTGTT TTCCTTATCA AACGCAGGAA GAGCCACTGT TTATAATGCA TCATATAGAC
    ATTACACTAT CAGTTTCTGG TAGCAACCTA CTACAGTCAT TTAAAGAGTC AATGGTGAAA
    GACAAAAAAA AGGAAAGAAA GCCTTCATCA GATGAGGAGA CTGAGAGTGA CAGTAATAGT
    GGCAGTGAGA GCGAAAGTGA AGAGGAAGCT TCTAAGCCTC GGAGGTTACG AAAACGAGTA
    GATTCTGACT CAGATTCTGA TGAAGAAAAT GATATAAATG CTGTGATGAA ATGTTTACCA
    GAAAACTCAG CTCCTTTAAT TGAATTTGCA AATGTGTCTC AAGGCATATT ATTACTTTTG
    ATGCTGAAAC AGCATTTAAA GAACCTTTGC GGATTCTCTG ATAGTAAAAT TCAGAAATAT
    TCTCCATCTG AGTCTGCAAA AGTTTATGAT AAAGCGATAA ACAGGAAGAC AGGAGTGCAT
    TTCCATCCAA AACAAACTCT GGATTTTCTA CGGAGTGATA TGGCTAATTC CAAGATCACT
    GAAGAAGTGA AGAGGAGTAT AGTAAAACAG TACTTAGATT TCAAGCTCCT TATGGAACAT
    TTGGATCCTG ATGAAGAAGA AGAAGATGGA GAGGTGTCAG CTAGCACAAA TGCTCGGAAC
    AAAGCAATTA CCTCGCTGCT TGGAGGAGGC AGCCCTAAAA ACAATGCAGC TGAGACAGAG
    GACGATGAAA GTGATGGGGA GGATAGAGGA GGAGGCACTT CAGGGTCATT GAGAAGGTCA
    AAACGAAATT CAGACTCTAC GGAGTTGGCT TCACAGATGA ATGAAAGTGT TGATGTCATG
    GATGTCATCG CTATTTGCTG TCCAAAGTAC AAAGATCGAC CACAAATTGC AAGAGTAGTA
    CAGAAAACCA GCAATGGCTT CAGTGTTCAG TGGATGGCAG GCTCCTACAG TGGCTCCTGG
    ACTGAGGCTA AGCGCCGTGA TGGCCGCAAA CTGGTGCCTT GGGTAGACAC TATTAAAGAG
    TCAGACATTA TTTACAAAAA AATTGCTCTA ACGAGTGCTA ATAAGCTGAC TAATAAAGTT
    GTTCAGACTT TACGATCGCT GTATGCCGCC AAGGATGGAA CTTCGAGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133010.1
    CDS3224..9394
    Translation

    Target ORF information:

    RefSeq Version XM_015277524.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277524.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG TAGCATAGAT CAGTCAGTGC TAAAGGAGTT GCCTCCTGAG
    CTCCTGGCAG AAATTGAATC CACCATGCCT CTTTGTGAAC GCGTGAAAAT GAACAAGCGC
    AAACGTAGCA CAGTTAATGA GAAACCAAAA TATGCTGAAA TCAGTTCTGA TGAAGACAAT
    GACAGTGAAG AAGCTTTTGA ATCTTCTCGA AAAAGACATA AAAAGGATAG AGATGAAGCT
    TGGGAGTACG AAGAGCATGA CAGAAGAAGT TCTGGTGACC ACAGGAGGAG TGGTCATTAT
    CATGAAGGAA GAAGGACTTC TGGTAGTGGC CGTTACCGCA ATCGCAGTCC CGATGACTCT
    GATCTGGATG ATTATTCTCC TCCTCCTAGC CTCAGTGAGG TGGCAAGAAA AATGAAGAAA
    AAAGAAAAGC AAAAGAAGAG GAGAGCTTAC GAGCCTAAAC TAACACCTGA AGAAATGATG
    GATTCTTCAA CCTTCAAAAG ATTTATTGCT TCAATAGAGA ATATTCTGGA AAATCTGGAA
    GACATGGATT TTACAACCTT GGGTGATGAT GATGAGATTC CACAGGAATT GCTTTTGGGA
    AAACATCAGC TTAATGAGTT GGGTAGTGAA TCTGCAAAAA TCAAGGCAAT GGGTATTATG
    GACAAGCTCT CAACAGATAA AATAGTAAAG GTCCTGAACA TTTTGGAGAA GAATATTCAA
    GACGGATCAA AGCTTTCTAC ATTACTTAAC CACAACAATG ACACTGAAGA TGAGGAGAGA
    TTATGGAGAG ATCTCATAAT GGAGAGAGTA ACAAAATCTG CAGATGCTTG TCTTACTGCT
    ATCAATATTA TGACATCACC AAATATGCCT AAAGCTGTTT ATATTGAGGA CGTAATAGAA
    AGAGTTATTC AGTACACAAA ATTTCATTTG CAGAACACTC TCTATCCTCA GTATGACCCT
    GTGTATAGAG TGGATCCTCT TGGTGGTGGT CTTTTAAGTT CAAAAGCAAA ACGTGCCAAG
    TGTTCCACCC ACAAACAAAG AGTTATAGTG ATGTTGTATA ACAAAGTTTG TGATATTGTC
    AGCAGTTTGT CAGAGTTGCT AGAGATACAA CTTCTTACTG ATACAACTAT TCTTCAGGTA
    TCGTCTATGG GGATAACACC TTTCTTTGTA GAAAATGTCA GTGAACTACA GCTATGTGCC
    ATCAAATTAG TGACTGCAGT CTTCTCCAGG TATGAAAAAC ATAGACAGCT AATACTAGAA
    GAAATTTTTA CTTCCCTTGC AAGACTACCA ACTAGCAAGA GGAGCCTGAG AAACTTCAGG
    TTGAATAGCA GTGACACAGA TGGAGAGCCT ATGTATATTC AGATGGTAAC AGCACTAGTT
    CTGCAGCTTA TTCAATGTGT CGTGCACTTA CCATCAGCAG AAAAAGATTC TAATTCAGAT
    GAGGAATTGA ACAAAAAAGT GGATCAAGAT GTGCTTATTA CTAACTCATA TGAAACAGCT
    ATGAGAACAG CACAAAATTT TCTTTCTATT TTTCTTAAGA AATGTGGTAG TAAACAAGGA
    GAAGAAGATT ACAGGCCTCT CTTTGAGAAC TTCATTCAAG ATCTTCTTTC CACAGTCAAT
    AAACCAGAAT GGCCAGCTGC AGAGTTGCTG CTTAGCTTAC TAGGAAGGTT ATTGGTTCAC
    CAGTTCAGTA ATAAATCAAC AGAAATGGCT TTAAGAGTTG CGTCACTTGA CTATCTTGGA
    ACCGTAGCTG CAAGACTGAG GAAAGATGCA GTCACTAGTA AAATGGATCA AGGATCTATA
    GCTAGAATCC TCAAACAGGC CTCAGGAGGA GAAGATGATA TTCAACAGCT ACAGAAGGCT
    TTATTGGATT ATCTGGATGA GAACACTGAA ACTGATGCAT CGTTAGTGTT TTCTCGGAAG
    TTTTATATAG CTCAGTGGTT TCGGGATACA ACTATGGAGA CAGAGAAGGC AATTAAATCT
    CAGAAGGATG AGGATTCATC TGAAGGAACT CATCATGCAA AAGATGTTGA GACCACAGGA
    CAAATCATGC ACAGAGCAGA AAGCCGCAAG AAGTTTCTCC GCAGCATCAT TAAAACTGCT
    CCATCTCAGT TCAGTACATT AAAGATTTAT TCTGACACTG TGGACTATGA AGATGCATGT
    TTGATTGTTC GCTACTTGGC CTCTATGAGG CCGTTTGCCC AGAGCTTCGA TATTTATTTA
    ACACAGATTC TGCGTGTACT TGGTGAAAAT GCAATTGCAG TTCGAACAAA AGCCATGAAG
    TGTTTGTCTG AGGTTGTTGC TGTAGATCCC AGTATTCTAG CCAGGCCGGA TATGCAGCGA
    GGTGTTCATG GACGGTTAAT GGATAACTCC ACCAGTGTAC GGGAAGCAGC TGTGGAGCTG
    CTTGGCCGAT TTGTGCTCTG TCGTCCTCAG CTTGCTGAGC AGTATTATGA CATGCTGATT
    GAAAGAATAT TGGATACTGG TATCAGTGTC AGAAAAAGAG TCATAAAGAT ACTTAGGGAT
    ATCTGCATTG AACAACCAAC GTTTCCCAAA ATTACAGAGA TGTGTGTGAA AATGATTCGG
    AGAGTCAATG ATGAAGAAGG CATTAAGAAA TTAGTAAATG AGACATTCCA GAAGCTGTGG
    TTTACTCCAA CTCCACACCA TGACAAAGAA GCAATGACTA GGAAAATACT GAACATCACT
    GATGTGGTGG CAGCTTGTAG GGATACAGGG TATGATTGGT TTGAGCAGCT TCTTCAAAAT
    CTATTGAAGT CAGAAGAGGA TGCTTCATAT AAACCTGTGA AGAAGGCTTG TACTCAACTT
    GTTGACAATT TGGTTGAGCA CATCCTTAAA TATGAGGAGT CTTTATCAGA CTCTGACAAC
    AAAGGAGTGA ATTCTGGTCG CTTGGTTGCT TGTATAACCA CTCTGTTCTT ATTCAGCAAA
    ATCAGGCCCC AGTTAATGGT CAAACATGCC ATGACCATGC AGCCATATCT GGCAACTAAA
    TGTAGTACTC AGAATGATTT CATGGTGATC TGCAATGTTG CAAAAATCTT GGAGCTTGTA
    GTGCCGTTAA TGGAACACCC AAGTGAGACC TTCCTTGCCA CTATTGAGGA AGACCTCATG
    AAACTCATCA TCAAATATGG CATGACAGTT GTTCAACATT GTGTGAGCTG CCTTGGGTCT
    GTTGTGAACA AGGTCACCCA GAATTACAAG TTTGTGTGGG CCTGTTTTAA TAGATACTAT
    GGTGCACTTT CAAAATTAAA AAGTCAACAC CAAGAAGACC CCAACAGTAC AATACTTACT
    GCCAATAAAC CAGCACTCCT TAGATCACTT TTCACTGTTG GAGCACTGTG TCGGCATTTT
    GATTTTGACC ATGAAGATTT TAAGGGCAAC AGTAAGGTTA ACATTAAGGA TAAAGTACTT
    GAACTGCTGA TGTATTTTAC AAAGCATTCA GACGAAGAAG TACAGACAAA AGCCATTATT
    GGCCTGGGAT TTGCATTTAT ACAACACCCA AGCTTAATGT TTGAACAGGA AGTAAAGACT
    CTGTACAACA GCATTCTTTC AGATAAGAAC TGTTCAGTAA ACCTAAAAAT CCAGGTGTTA
    AAAAACCTCC AGACCTACTT GCAGGAGGAG GACACACGTA TGCAGCAGGC AGACAGAGAC
    TGGAAAAAAG TAGCAAAACA GGAAGACCTG AAAGAAATGG GTGATATTTC CTCAGGGATG
    AGTAGTTCCA TCATGCAGCT GTATCTCAAA CAGGTCCTTG AGGCTTTCTT TCATACCCAG
    TCCAATGTAC GCCACTTTGC TCTAAATGTC ATTGCACTGA CGTTAAACCA GGGTCTCATC
    CATCCTGTTC AGTGTGTGCC ATACTTAATT GCTATGGGCA CAGATCCAGA GCCTTCTATG
    CGAAACAAAG CTGACCAGCA GTTGGTGGAA ATAGACAAAA AGTATGCTGG TTTCATTCAC
    ATGAAAGCAG TGGCTGGTAT GAAGATGTCT TACCAGGTAC AACAGGCAAT CAATACCTGC
    CCAAAGGATC CTGTAAGAGG CTTTAGACAC GATGAATCAT CCAGTGCATT GTGTTCACAC
    CTATATTCTA TGATCCGAGG GAACCGTCAA CACAGACGAG CATTTCTAAT TTCTTTACTC
    AATCTCTTCG ATGATACTGC AAAAACAGAG GTGAATATGC TCTTGTACAT AGCAGACAAT
    CTAGCCTGTT TTCCTTATCA AACGCAGGAA GAGCCACTGT TTATAATGCA TCATATAGAC
    ATTACACTAT CAGTTTCTGG TAGCAACCTA CTACAGTCAT TTAAAGAGTC AATGGTGAAA
    GACAAAAAAA AGGAAAGAAA GCCTTCATCA GATGAGGAGA CTGAGAGTGA CAGTAATAGT
    GGCAGTGAGA GCGAAAGTGA AGAGGAAGCT TCTAAGCCTC GGAGGTTACG AAAACGAGTA
    GATTCTGACT CAGATTCTGA TGAAGAAAAT GATATAAATG CTGTGATGAA ATGTTTACCA
    GAAAACTCAG CTCCTTTAAT TGAATTTGCA AATGTGTCTC AAGGCATATT ATTACTTTTG
    ATGCTGAAAC AGCATTTAAA GAACCTTTGC GGATTCTCTG ATAGTAAAAT TCAGAAATAT
    TCTCCATCTG AGTCTGCAAA AGTTTATGAT AAAGCGATAA ACAGGAAGAC AGGAGTGCAT
    TTCCATCCAA AACAAACTCT GGATTTTCTA CGGAGTGATA TGGCTAATTC CAAGATCACT
    GAAGAAGTGA AGAGGAGTAT AGTAAAACAG TACTTAGATT TCAAGCTCCT TATGGAACAT
    TTGGATCCTG ATGAAGAAGA AGAAGATGGA GAGGTGTCAG CTAGCACAAA TGCTCGGAAC
    AAAGCAATTA CCTCGCTGCT TGGAGGAGGC AGCCCTAAAA ACAATGCAGC TGAGACAGAG
    GACGATGAAA GTGATGGGGA GGATAGAGGA GGAGGCACTT CAGGGTCATT GAGAAGGTCA
    AAACGAAATT CAGACTCTAC GGAGTTGGCT TCACAGATGA ATGAAAGTGT TGATGTCATG
    GATGTCATCG CTATTTGCTG TCCAAAGTAC AAAGATCGAC CACAAATTGC AAGAGTAGTA
    CAGAAAACCA GCAATGGCTT CAGTGTTCAG TGGATGGCAG GCTCCTACAG TGGCTCCTGG
    ACTGAGGCTA AGCGCCGTGA TGGCCGCAAA CTGGTGCCTT GGGTAGACAC TATTAAAGAG
    TCAGACATTA TTTACAAAAA AATTGCTCTA ACGAGTGCTA ATAAGCTGAC TAATAAAGTT
    GTTCAGACTT TACGATCGCT GTATGCCGCC AAGGATGGAA CTTCGAGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa20949
    Clone ID Related Accession (Same CDS sequence) XM_004937195.2 , XM_004937195.3
    Accession Version XM_004937195.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6804bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product nipped-B-like protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004937195.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGGTAGCATA
    GATCAGTCAG TGCTAAAGGA GTTGCCTCCT GAGCTCCTGG CAGAAATTGA ATCCACCATG
    CCTCTTTGTG AACGCGTGAA AATGAACAAG CGCAAACGTA GCACAGTTAA TGAGAAACCA
    AAATATGCTG AAATCAGTTC TGATGAAGAC AATGACAGTG AAGAAGCTTT TGAATCTTCT
    CGAAAAAGAC ATAAAAAGGA TAGAGATGAA GCTTGGGAGT ACGAAGAGCA TGACAGAAGA
    AGTTCTGGTG ACCACAGGAG GAGTGGTCAT TATCATGAAG GAAGAAGGAC TTCTGGTAGT
    GGCCGTTACC GCAATCGCAG TCCCGATGAC TCTGATCTGG ATGATTATTC TCCTCCTCCT
    AGCCTCAGTG AGGTGGCAAG AAAAATGAAG AAAAAAGAAA AGCAAAAGAA GAGGAGAGCT
    TACGAGCCTA AACTAACACC TGAAGAAATG ATGGATTCTT CAACCTTCAA AAGATTTATT
    GCTTCAATAG AGAATATTCT GGAAAATCTG GAAGACATGG ATTTTACAAC CTTGGGTGAT
    GATGATGAGA TTCCACAGGA ATTGCTTTTG GGAAAACATC AGCTTAATGA GTTGGGTAGT
    GAATCTGCAA AAATCAAGGC AATGGGTATT ATGGACAAGC TCTCAACAGA TAAAATAGTA
    AAGGTCCTGA ACATTTTGGA GAAGAATATT CAAGACGGAT CAAAGCTTTC TACATTACTT
    AACCACAACA ATGACACTGA AGATGAGGAG AGATTATGGA GAGATCTCAT AATGGAGAGA
    GTAACAAAAT CTGCAGATGC TTGTCTTACT GCTATCAATA TTATGACATC ACCAAATATG
    CCTAAAGCTG TTTATATTGA GGACGTAATA GAAAGAGTTA TTCAGTACAC AAAATTTCAT
    TTGCAGAACA CTCTCTATCC TCAGTATGAC CCTGTGTATA GAGTGGATCC TCTTGGTGGT
    GGTCTTTTAA GTTCAAAAGC AAAACGTGCC AAGTGTTCCA CCCACAAACA AAGAGTTATA
    GTGATGTTGT ATAACAAAGT TTGTGATATT GTCAGCAGTT TGTCAGAGTT GCTAGAGATA
    CAACTTCTTA CTGATACAAC TATTCTTCAG GTATCGTCTA TGGGGATAAC ACCTTTCTTT
    GTAGAAAATG TCAGTGAACT ACAGCTATGT GCCATCAAAT TAGTGACTGC AGTCTTCTCC
    AGGTATGAAA AACATAGACA GCTAATACTA GAAGAAATTT TTACTTCCCT TGCAAGACTA
    CCAACTAGCA AGAGGAGCCT GAGAAACTTC AGGTTGAATA GCAGTGACAC AGATGGAGAG
    CCTATGTATA TTCAGATGGT AACAGCACTA GTTCTGCAGC TTATTCAATG TGTCGTGCAC
    TTACCATCAG CAGAAAAAGA TTCTAATTCA GATGAGGAAT TGAACAAAAA AGTGGATCAA
    GATGTGCTTA TTACTAACTC ATATGAAACA GCTATGAGAA CAGCACAAAA TTTTCTTTCT
    ATTTTTCTTA AGAAATGTGG TAGTAAACAA GGAGAAGAAG ATTACAGGCC TCTCTTTGAG
    AACTTCATTC AAGATCTTCT TTCCACAGTC AATAAACCAG AATGGCCAGC TGCAGAGTTG
    CTGCTTAGCT TACTAGGAAG GTTATTGGTT CACCAGTTCA GTAATAAATC AACAGAAATG
    GCTTTAAGAG TTGCGTCACT TGACTATCTT GGAACCGTAG CTGCAAGACT GAGGAAAGAT
    GCAGTCACTA GTAAAATGGA TCAAGGATCT ATAGCTAGAA TCCTCAAACA GGCCTCAGGA
    GGAGAAGATG ATATTCAACA GCTACAGAAG GCTTTATTGG ATTATCTGGA TGAGAACACT
    GAAACTGATG CATCGTTAGT GTTTTCTCGG AAGTTTTATA TAGCTCAGTG GTTTCGGGAT
    ACAACTATGG AGACAGAGAA GGCAATTAAA TCTCAGAAGG ATGAGGATTC ATCTGAAGGA
    ACTCATCATG CAAAAGATGT TGAGACCACA GGACAAATCA TGCACAGAGC AGAAAGCCGC
    AAGAAGTTTC TCCGCAGCAT CATTAAAACT GCTCCATCTC AGTTCAGTAC ATTAAAGATT
    TATTCTGACA CTGTGGACTA TGAAGATGCA TGTTTGATTG TTCGCTACTT GGCCTCTATG
    AGGCCGTTTG CCCAGAGCTT CGATATTTAT TTAACACAGA TTCTGCGTGT ACTTGGTGAA
    AATGCAATTG CAGTTCGAAC AAAAGCCATG AAGTGTTTGT CTGAGGTTGT TGCTGTAGAT
    CCCAGTATTC TAGCCAGGCC GGATATGCAG CGAGGTGTTC ATGGACGGTT AATGGATAAC
    TCCACCAGTG TACGGGAAGC AGCTGTGGAG CTGCTTGGCC GATTTGTGCT CTGTCGTCCT
    CAGCTTGCTG AGCAGTATTA TGACATGCTG ATTGAAAGAA TATTGGATAC TGGTATCAGT
    GTCAGAAAAA GAGTCATAAA GATACTTAGG GATATCTGCA TTGAACAACC AACGTTTCCC
    AAAATTACAG AGATGTGTGT GAAAATGATT CGGAGAGTCA ATGATGAAGA AGGCATTAAG
    AAATTAGTAA ATGAGACATT CCAGAAGCTG TGGTTTACTC CAACTCCACA CCATGACAAA
    GAAGCAATGA CTAGGAAAAT ACTGAACATC ACTGATGTGG TGGCAGCTTG TAGGGATACA
    GGGTATGATT GGTTTGAGCA GCTTCTTCAA AATCTATTGA AGTCAGAAGA GGATGCTTCA
    TATAAACCTG TGAAGAAGGC TTGTACTCAA CTTGTTGACA ATTTGGTTGA GCACATCCTT
    AAATATGAGG AGTCTTTATC AGACTCTGAC AACAAAGGAG TGAATTCTGG TCGCTTGGTT
    GCTTGTATAA CCACTCTGTT CTTATTCAGC AAAATCAGGC CCCAGTTAAT GGTCAAACAT
    GCCATGACCA TGCAGCCATA TCTGGCAACT AAATGTAGTA CTCAGAATGA TTTCATGGTG
    ATCTGCAATG TTGCAAAAAT CTTGGAGCTT GTAGTGCCGT TAATGGAACA CCCAAGTGAG
    ACCTTCCTTG CCACTATTGA GGAAGACCTC ATGAAACTCA TCATCAAATA TGGCATGACA
    GTTGTTCAAC ATTGTGTGAG CTGCCTTGGG TCTGTTGTGA ACAAGGTCAC CCAGAATTAC
    AAGTTTGTGT GGGCCTGTTT TAATAGATAC TATGGTGCAC TTTCAAAATT AAAAAGTCAA
    CACCAAGAAG ACCCCAACAG TACAATACTT ACTGCCAATA AACCAGCACT CCTTAGATCA
    CTTTTCACTG TTGGAGCACT GTGTCGGCAT TTTGATTTTG ACCATGAAGA TTTTAAGGGC
    AACAGTAAGG TTAACATTAA GGATAAAGTA CTTGAACTGC TGATGTATTT TACAAAGCAT
    TCAGACGAAG AAGTACAGAC AAAAGCCATT ATTGGCCTGG GATTTGCATT TATACAACAC
    CCAAGCTTAA TGTTTGAACA GGAAGTAAAG ACTCTGTACA ACAGCATTCT TTCAGATAAG
    AACTGTTCAG TAAACCTAAA AATCCAGGTG TTAAAAAACC TCCAGACCTA CTTGCAGGAG
    GAGGACACAC GTATGCAGCA GGCAGACAGA GACTGGAAAA AAGTAGCAAA ACAGGAAGAC
    CTGAAAGAAA TGGGTGATAT TTCCTCAGGG ATGAGTAGTT CCATCATGCA GCTGTATCTC
    AAACAGGTCC TTGAGGCTTT CTTTCATACC CAGTCCAATG TACGCCACTT TGCTCTAAAT
    GTCATTGCAC TGACGTTAAA CCAGGGTCTC ATCCATCCTG TTCAGTGTGT GCCATACTTA
    ATTGCTATGG GCACAGATCC AGAGCCTTCT ATGCGAAACA AAGCTGACCA GCAGTTGGTG
    GAAATAGACA AAAAGTATGC TGGTTTCATT CACATGAAAG CAGTGGCTGG TATGAAGATG
    TCTTACCAGG TACAACAGGC AATCAATACC TGCCCAAAGG ATCCTGTAAG AGGCTTTAGA
    CACGATGAAT CATCCAGTGC ATTGTGTTCA CACCTATATT CTATGATCCG AGGGAACCGT
    CAACACAGAC GAGCATTTCT AATTTCTTTA CTCAATCTCT TCGATGATAC TGCAAAAACA
    GAGGTGAATA TGCTCTTGTA CATAGCAGAC AATCTAGCCT GTTTTCCTTA TCAAACGCAG
    GAAGAGCCAC TGTTTATAAT GCATCATATA GACATTACAC TATCAGTTTC TGGTAGCAAC
    CTACTACAGT CATTTAAAGA GTCAATGGTG AAAGACAAAA AAAAGGAAAG AAAGCCTTCA
    TCAGATGAGG AGACTGAGAG TGACAGTAAT AGTGGCAGTG AGAGCGAAAG TGAAGAGGAA
    GCTTCTAAGC CTCGGAGGTT ACGAAAACGA GTAGATTCTG ACTCAGATTC TGATGAAGAA
    AATGATATAA ATGCTGTGAT GAAATGTTTA CCAGAAAACT CAGCTCCTTT AATTGAATTT
    GCAAATGTGT CTCAAGGCAT ATTATTACTT TTGATGCTGA AACAGCATTT AAAGAACCTT
    TGCGGATTCT CTGATAGTAA AATTCAGAAA TATTCTCCAT CTGAGTCTGC AAAAGTTTAT
    GATAAAGCGA TAAACAGGAA GACAGGAGTG CATTTCCATC CAAAACAAAC TCTGGATTTT
    CTACGGAGTG ATATGGCTAA TTCCAAGATC ACTGAAGAAG TGAAGAGGAG TATAGTAAAA
    CAGTACTTAG ATTTCAAGCT CCTTATGGAA CATTTGGATC CTGATGAAGA AGAAGAAGAT
    GGAGAGGTGT CAGCTAGCAC AAATGCTCGG AACAAAGCAA TTACCTCGCT GCTTGGAGGA
    GGCAGCCCTA AAAACAATGC AGCTGAGACA GAGGACGATG AAAGTGATGG GGAGGATAGA
    GGAGGAGGCA CTTCAGGGTC ATTGAGAAGG TCAAAACGAA ATTCAGACTC TACGGAGTTG
    GCTTCACAGA TGAATGAAAG TGTTGATGTC ATGGATGTCA TCGCTATTTG CTGTCCAAAG
    TACAAAGATC GACCACAAAT TGCAAGAGTA GTACAGAAAA CCAGCAATGG CTTCAGTGTT
    CAGTGGATGG CAGGCTCCTA CAGTGGCTCC TGGACTGAGG CTAAGCGCCG TGATGGCCGC
    AAACTGGTGC CTTGGGTAGA CACTATTAAA GAGTCAGACA TTATTTACAA AAAAATTGCT
    CTAACGAGTG CTAATAAGCT GACTAATAAA GTTGTTCAGA CTTTACGATC GCTGTATGCC
    GCCAAGGATG GAACTTCGAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004937252.1
    CDS3243..10046
    Translation

    Target ORF information:

    RefSeq Version XM_004937195.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004937195.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGGTAGCATA
    GATCAGTCAG TGCTAAAGGA GTTGCCTCCT GAGCTCCTGG CAGAAATTGA ATCCACCATG
    CCTCTTTGTG AACGCGTGAA AATGAACAAG CGCAAACGTA GCACAGTTAA TGAGAAACCA
    AAATATGCTG AAATCAGTTC TGATGAAGAC AATGACAGTG AAGAAGCTTT TGAATCTTCT
    CGAAAAAGAC ATAAAAAGGA TAGAGATGAA GCTTGGGAGT ACGAAGAGCA TGACAGAAGA
    AGTTCTGGTG ACCACAGGAG GAGTGGTCAT TATCATGAAG GAAGAAGGAC TTCTGGTAGT
    GGCCGTTACC GCAATCGCAG TCCCGATGAC TCTGATCTGG ATGATTATTC TCCTCCTCCT
    AGCCTCAGTG AGGTGGCAAG AAAAATGAAG AAAAAAGAAA AGCAAAAGAA GAGGAGAGCT
    TACGAGCCTA AACTAACACC TGAAGAAATG ATGGATTCTT CAACCTTCAA AAGATTTATT
    GCTTCAATAG AGAATATTCT GGAAAATCTG GAAGACATGG ATTTTACAAC CTTGGGTGAT
    GATGATGAGA TTCCACAGGA ATTGCTTTTG GGAAAACATC AGCTTAATGA GTTGGGTAGT
    GAATCTGCAA AAATCAAGGC AATGGGTATT ATGGACAAGC TCTCAACAGA TAAAATAGTA
    AAGGTCCTGA ACATTTTGGA GAAGAATATT CAAGACGGAT CAAAGCTTTC TACATTACTT
    AACCACAACA ATGACACTGA AGATGAGGAG AGATTATGGA GAGATCTCAT AATGGAGAGA
    GTAACAAAAT CTGCAGATGC TTGTCTTACT GCTATCAATA TTATGACATC ACCAAATATG
    CCTAAAGCTG TTTATATTGA GGACGTAATA GAAAGAGTTA TTCAGTACAC AAAATTTCAT
    TTGCAGAACA CTCTCTATCC TCAGTATGAC CCTGTGTATA GAGTGGATCC TCTTGGTGGT
    GGTCTTTTAA GTTCAAAAGC AAAACGTGCC AAGTGTTCCA CCCACAAACA AAGAGTTATA
    GTGATGTTGT ATAACAAAGT TTGTGATATT GTCAGCAGTT TGTCAGAGTT GCTAGAGATA
    CAACTTCTTA CTGATACAAC TATTCTTCAG GTATCGTCTA TGGGGATAAC ACCTTTCTTT
    GTAGAAAATG TCAGTGAACT ACAGCTATGT GCCATCAAAT TAGTGACTGC AGTCTTCTCC
    AGGTATGAAA AACATAGACA GCTAATACTA GAAGAAATTT TTACTTCCCT TGCAAGACTA
    CCAACTAGCA AGAGGAGCCT GAGAAACTTC AGGTTGAATA GCAGTGACAC AGATGGAGAG
    CCTATGTATA TTCAGATGGT AACAGCACTA GTTCTGCAGC TTATTCAATG TGTCGTGCAC
    TTACCATCAG CAGAAAAAGA TTCTAATTCA GATGAGGAAT TGAACAAAAA AGTGGATCAA
    GATGTGCTTA TTACTAACTC ATATGAAACA GCTATGAGAA CAGCACAAAA TTTTCTTTCT
    ATTTTTCTTA AGAAATGTGG TAGTAAACAA GGAGAAGAAG ATTACAGGCC TCTCTTTGAG
    AACTTCATTC AAGATCTTCT TTCCACAGTC AATAAACCAG AATGGCCAGC TGCAGAGTTG
    CTGCTTAGCT TACTAGGAAG GTTATTGGTT CACCAGTTCA GTAATAAATC AACAGAAATG
    GCTTTAAGAG TTGCGTCACT TGACTATCTT GGAACCGTAG CTGCAAGACT GAGGAAAGAT
    GCAGTCACTA GTAAAATGGA TCAAGGATCT ATAGCTAGAA TCCTCAAACA GGCCTCAGGA
    GGAGAAGATG ATATTCAACA GCTACAGAAG GCTTTATTGG ATTATCTGGA TGAGAACACT
    GAAACTGATG CATCGTTAGT GTTTTCTCGG AAGTTTTATA TAGCTCAGTG GTTTCGGGAT
    ACAACTATGG AGACAGAGAA GGCAATTAAA TCTCAGAAGG ATGAGGATTC ATCTGAAGGA
    ACTCATCATG CAAAAGATGT TGAGACCACA GGACAAATCA TGCACAGAGC AGAAAGCCGC
    AAGAAGTTTC TCCGCAGCAT CATTAAAACT GCTCCATCTC AGTTCAGTAC ATTAAAGATT
    TATTCTGACA CTGTGGACTA TGAAGATGCA TGTTTGATTG TTCGCTACTT GGCCTCTATG
    AGGCCGTTTG CCCAGAGCTT CGATATTTAT TTAACACAGA TTCTGCGTGT ACTTGGTGAA
    AATGCAATTG CAGTTCGAAC AAAAGCCATG AAGTGTTTGT CTGAGGTTGT TGCTGTAGAT
    CCCAGTATTC TAGCCAGGCC GGATATGCAG CGAGGTGTTC ATGGACGGTT AATGGATAAC
    TCCACCAGTG TACGGGAAGC AGCTGTGGAG CTGCTTGGCC GATTTGTGCT CTGTCGTCCT
    CAGCTTGCTG AGCAGTATTA TGACATGCTG ATTGAAAGAA TATTGGATAC TGGTATCAGT
    GTCAGAAAAA GAGTCATAAA GATACTTAGG GATATCTGCA TTGAACAACC AACGTTTCCC
    AAAATTACAG AGATGTGTGT GAAAATGATT CGGAGAGTCA ATGATGAAGA AGGCATTAAG
    AAATTAGTAA ATGAGACATT CCAGAAGCTG TGGTTTACTC CAACTCCACA CCATGACAAA
    GAAGCAATGA CTAGGAAAAT ACTGAACATC ACTGATGTGG TGGCAGCTTG TAGGGATACA
    GGGTATGATT GGTTTGAGCA GCTTCTTCAA AATCTATTGA AGTCAGAAGA GGATGCTTCA
    TATAAACCTG TGAAGAAGGC TTGTACTCAA CTTGTTGACA ATTTGGTTGA GCACATCCTT
    AAATATGAGG AGTCTTTATC AGACTCTGAC AACAAAGGAG TGAATTCTGG TCGCTTGGTT
    GCTTGTATAA CCACTCTGTT CTTATTCAGC AAAATCAGGC CCCAGTTAAT GGTCAAACAT
    GCCATGACCA TGCAGCCATA TCTGGCAACT AAATGTAGTA CTCAGAATGA TTTCATGGTG
    ATCTGCAATG TTGCAAAAAT CTTGGAGCTT GTAGTGCCGT TAATGGAACA CCCAAGTGAG
    ACCTTCCTTG CCACTATTGA GGAAGACCTC ATGAAACTCA TCATCAAATA TGGCATGACA
    GTTGTTCAAC ATTGTGTGAG CTGCCTTGGG TCTGTTGTGA ACAAGGTCAC CCAGAATTAC
    AAGTTTGTGT GGGCCTGTTT TAATAGATAC TATGGTGCAC TTTCAAAATT AAAAAGTCAA
    CACCAAGAAG ACCCCAACAG TACAATACTT ACTGCCAATA AACCAGCACT CCTTAGATCA
    CTTTTCACTG TTGGAGCACT GTGTCGGCAT TTTGATTTTG ACCATGAAGA TTTTAAGGGC
    AACAGTAAGG TTAACATTAA GGATAAAGTA CTTGAACTGC TGATGTATTT TACAAAGCAT
    TCAGACGAAG AAGTACAGAC AAAAGCCATT ATTGGCCTGG GATTTGCATT TATACAACAC
    CCAAGCTTAA TGTTTGAACA GGAAGTAAAG ACTCTGTACA ACAGCATTCT TTCAGATAAG
    AACTGTTCAG TAAACCTAAA AATCCAGGTG TTAAAAAACC TCCAGACCTA CTTGCAGGAG
    GAGGACACAC GTATGCAGCA GGCAGACAGA GACTGGAAAA AAGTAGCAAA ACAGGAAGAC
    CTGAAAGAAA TGGGTGATAT TTCCTCAGGG ATGAGTAGTT CCATCATGCA GCTGTATCTC
    AAACAGGTCC TTGAGGCTTT CTTTCATACC CAGTCCAATG TACGCCACTT TGCTCTAAAT
    GTCATTGCAC TGACGTTAAA CCAGGGTCTC ATCCATCCTG TTCAGTGTGT GCCATACTTA
    ATTGCTATGG GCACAGATCC AGAGCCTTCT ATGCGAAACA AAGCTGACCA GCAGTTGGTG
    GAAATAGACA AAAAGTATGC TGGTTTCATT CACATGAAAG CAGTGGCTGG TATGAAGATG
    TCTTACCAGG TACAACAGGC AATCAATACC TGCCCAAAGG ATCCTGTAAG AGGCTTTAGA
    CACGATGAAT CATCCAGTGC ATTGTGTTCA CACCTATATT CTATGATCCG AGGGAACCGT
    CAACACAGAC GAGCATTTCT AATTTCTTTA CTCAATCTCT TCGATGATAC TGCAAAAACA
    GAGGTGAATA TGCTCTTGTA CATAGCAGAC AATCTAGCCT GTTTTCCTTA TCAAACGCAG
    GAAGAGCCAC TGTTTATAAT GCATCATATA GACATTACAC TATCAGTTTC TGGTAGCAAC
    CTACTACAGT CATTTAAAGA GTCAATGGTG AAAGACAAAA AAAAGGAAAG AAAGCCTTCA
    TCAGATGAGG AGACTGAGAG TGACAGTAAT AGTGGCAGTG AGAGCGAAAG TGAAGAGGAA
    GCTTCTAAGC CTCGGAGGTT ACGAAAACGA GTAGATTCTG ACTCAGATTC TGATGAAGAA
    AATGATATAA ATGCTGTGAT GAAATGTTTA CCAGAAAACT CAGCTCCTTT AATTGAATTT
    GCAAATGTGT CTCAAGGCAT ATTATTACTT TTGATGCTGA AACAGCATTT AAAGAACCTT
    TGCGGATTCT CTGATAGTAA AATTCAGAAA TATTCTCCAT CTGAGTCTGC AAAAGTTTAT
    GATAAAGCGA TAAACAGGAA GACAGGAGTG CATTTCCATC CAAAACAAAC TCTGGATTTT
    CTACGGAGTG ATATGGCTAA TTCCAAGATC ACTGAAGAAG TGAAGAGGAG TATAGTAAAA
    CAGTACTTAG ATTTCAAGCT CCTTATGGAA CATTTGGATC CTGATGAAGA AGAAGAAGAT
    GGAGAGGTGT CAGCTAGCAC AAATGCTCGG AACAAAGCAA TTACCTCGCT GCTTGGAGGA
    GGCAGCCCTA AAAACAATGC AGCTGAGACA GAGGACGATG AAAGTGATGG GGAGGATAGA
    GGAGGAGGCA CTTCAGGGTC ATTGAGAAGG TCAAAACGAA ATTCAGACTC TACGGAGTTG
    GCTTCACAGA TGAATGAAAG TGTTGATGTC ATGGATGTCA TCGCTATTTG CTGTCCAAAG
    TACAAAGATC GACCACAAAT TGCAAGAGTA GTACAGAAAA CCAGCAATGG CTTCAGTGTT
    CAGTGGATGG CAGGCTCCTA CAGTGGCTCC TGGACTGAGG CTAAGCGCCG TGATGGCCGC
    AAACTGGTGC CTTGGGTAGA CACTATTAAA GAGTCAGACA TTATTTACAA AAAAATTGCT
    CTAACGAGTG CTAATAAGCT GACTAATAAA GTTGTTCAGA CTTTACGATC GCTGTATGCC
    GCCAAGGATG GAACTTCGAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa20949
    Clone ID Related Accession (Same CDS sequence) XM_004937195.2 , XM_004937195.3
    Accession Version XM_004937195.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6804bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product nipped-B-like protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004937195.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGGTAGCATA
    GATCAGTCAG TGCTAAAGGA GTTGCCTCCT GAGCTCCTGG CAGAAATTGA ATCCACCATG
    CCTCTTTGTG AACGCGTGAA AATGAACAAG CGCAAACGTA GCACAGTTAA TGAGAAACCA
    AAATATGCTG AAATCAGTTC TGATGAAGAC AATGACAGTG AAGAAGCTTT TGAATCTTCT
    CGAAAAAGAC ATAAAAAGGA TAGAGATGAA GCTTGGGAGT ACGAAGAGCA TGACAGAAGA
    AGTTCTGGTG ACCACAGGAG GAGTGGTCAT TATCATGAAG GAAGAAGGAC TTCTGGTAGT
    GGCCGTTACC GCAATCGCAG TCCCGATGAC TCTGATCTGG ATGATTATTC TCCTCCTCCT
    AGCCTCAGTG AGGTGGCAAG AAAAATGAAG AAAAAAGAAA AGCAAAAGAA GAGGAGAGCT
    TACGAGCCTA AACTAACACC TGAAGAAATG ATGGATTCTT CAACCTTCAA AAGATTTATT
    GCTTCAATAG AGAATATTCT GGAAAATCTG GAAGACATGG ATTTTACAAC CTTGGGTGAT
    GATGATGAGA TTCCACAGGA ATTGCTTTTG GGAAAACATC AGCTTAATGA GTTGGGTAGT
    GAATCTGCAA AAATCAAGGC AATGGGTATT ATGGACAAGC TCTCAACAGA TAAAATAGTA
    AAGGTCCTGA ACATTTTGGA GAAGAATATT CAAGACGGAT CAAAGCTTTC TACATTACTT
    AACCACAACA ATGACACTGA AGATGAGGAG AGATTATGGA GAGATCTCAT AATGGAGAGA
    GTAACAAAAT CTGCAGATGC TTGTCTTACT GCTATCAATA TTATGACATC ACCAAATATG
    CCTAAAGCTG TTTATATTGA GGACGTAATA GAAAGAGTTA TTCAGTACAC AAAATTTCAT
    TTGCAGAACA CTCTCTATCC TCAGTATGAC CCTGTGTATA GAGTGGATCC TCTTGGTGGT
    GGTCTTTTAA GTTCAAAAGC AAAACGTGCC AAGTGTTCCA CCCACAAACA AAGAGTTATA
    GTGATGTTGT ATAACAAAGT TTGTGATATT GTCAGCAGTT TGTCAGAGTT GCTAGAGATA
    CAACTTCTTA CTGATACAAC TATTCTTCAG GTATCGTCTA TGGGGATAAC ACCTTTCTTT
    GTAGAAAATG TCAGTGAACT ACAGCTATGT GCCATCAAAT TAGTGACTGC AGTCTTCTCC
    AGGTATGAAA AACATAGACA GCTAATACTA GAAGAAATTT TTACTTCCCT TGCAAGACTA
    CCAACTAGCA AGAGGAGCCT GAGAAACTTC AGGTTGAATA GCAGTGACAC AGATGGAGAG
    CCTATGTATA TTCAGATGGT AACAGCACTA GTTCTGCAGC TTATTCAATG TGTCGTGCAC
    TTACCATCAG CAGAAAAAGA TTCTAATTCA GATGAGGAAT TGAACAAAAA AGTGGATCAA
    GATGTGCTTA TTACTAACTC ATATGAAACA GCTATGAGAA CAGCACAAAA TTTTCTTTCT
    ATTTTTCTTA AGAAATGTGG TAGTAAACAA GGAGAAGAAG ATTACAGGCC TCTCTTTGAG
    AACTTCATTC AAGATCTTCT TTCCACAGTC AATAAACCAG AATGGCCAGC TGCAGAGTTG
    CTGCTTAGCT TACTAGGAAG GTTATTGGTT CACCAGTTCA GTAATAAATC AACAGAAATG
    GCTTTAAGAG TTGCGTCACT TGACTATCTT GGAACCGTAG CTGCAAGACT GAGGAAAGAT
    GCAGTCACTA GTAAAATGGA TCAAGGATCT ATAGCTAGAA TCCTCAAACA GGCCTCAGGA
    GGAGAAGATG ATATTCAACA GCTACAGAAG GCTTTATTGG ATTATCTGGA TGAGAACACT
    GAAACTGATG CATCGTTAGT GTTTTCTCGG AAGTTTTATA TAGCTCAGTG GTTTCGGGAT
    ACAACTATGG AGACAGAGAA GGCAATTAAA TCTCAGAAGG ATGAGGATTC ATCTGAAGGA
    ACTCATCATG CAAAAGATGT TGAGACCACA GGACAAATCA TGCACAGAGC AGAAAGCCGC
    AAGAAGTTTC TCCGCAGCAT CATTAAAACT GCTCCATCTC AGTTCAGTAC ATTAAAGATT
    TATTCTGACA CTGTGGACTA TGAAGATGCA TGTTTGATTG TTCGCTACTT GGCCTCTATG
    AGGCCGTTTG CCCAGAGCTT CGATATTTAT TTAACACAGA TTCTGCGTGT ACTTGGTGAA
    AATGCAATTG CAGTTCGAAC AAAAGCCATG AAGTGTTTGT CTGAGGTTGT TGCTGTAGAT
    CCCAGTATTC TAGCCAGGCC GGATATGCAG CGAGGTGTTC ATGGACGGTT AATGGATAAC
    TCCACCAGTG TACGGGAAGC AGCTGTGGAG CTGCTTGGCC GATTTGTGCT CTGTCGTCCT
    CAGCTTGCTG AGCAGTATTA TGACATGCTG ATTGAAAGAA TATTGGATAC TGGTATCAGT
    GTCAGAAAAA GAGTCATAAA GATACTTAGG GATATCTGCA TTGAACAACC AACGTTTCCC
    AAAATTACAG AGATGTGTGT GAAAATGATT CGGAGAGTCA ATGATGAAGA AGGCATTAAG
    AAATTAGTAA ATGAGACATT CCAGAAGCTG TGGTTTACTC CAACTCCACA CCATGACAAA
    GAAGCAATGA CTAGGAAAAT ACTGAACATC ACTGATGTGG TGGCAGCTTG TAGGGATACA
    GGGTATGATT GGTTTGAGCA GCTTCTTCAA AATCTATTGA AGTCAGAAGA GGATGCTTCA
    TATAAACCTG TGAAGAAGGC TTGTACTCAA CTTGTTGACA ATTTGGTTGA GCACATCCTT
    AAATATGAGG AGTCTTTATC AGACTCTGAC AACAAAGGAG TGAATTCTGG TCGCTTGGTT
    GCTTGTATAA CCACTCTGTT CTTATTCAGC AAAATCAGGC CCCAGTTAAT GGTCAAACAT
    GCCATGACCA TGCAGCCATA TCTGGCAACT AAATGTAGTA CTCAGAATGA TTTCATGGTG
    ATCTGCAATG TTGCAAAAAT CTTGGAGCTT GTAGTGCCGT TAATGGAACA CCCAAGTGAG
    ACCTTCCTTG CCACTATTGA GGAAGACCTC ATGAAACTCA TCATCAAATA TGGCATGACA
    GTTGTTCAAC ATTGTGTGAG CTGCCTTGGG TCTGTTGTGA ACAAGGTCAC CCAGAATTAC
    AAGTTTGTGT GGGCCTGTTT TAATAGATAC TATGGTGCAC TTTCAAAATT AAAAAGTCAA
    CACCAAGAAG ACCCCAACAG TACAATACTT ACTGCCAATA AACCAGCACT CCTTAGATCA
    CTTTTCACTG TTGGAGCACT GTGTCGGCAT TTTGATTTTG ACCATGAAGA TTTTAAGGGC
    AACAGTAAGG TTAACATTAA GGATAAAGTA CTTGAACTGC TGATGTATTT TACAAAGCAT
    TCAGACGAAG AAGTACAGAC AAAAGCCATT ATTGGCCTGG GATTTGCATT TATACAACAC
    CCAAGCTTAA TGTTTGAACA GGAAGTAAAG ACTCTGTACA ACAGCATTCT TTCAGATAAG
    AACTGTTCAG TAAACCTAAA AATCCAGGTG TTAAAAAACC TCCAGACCTA CTTGCAGGAG
    GAGGACACAC GTATGCAGCA GGCAGACAGA GACTGGAAAA AAGTAGCAAA ACAGGAAGAC
    CTGAAAGAAA TGGGTGATAT TTCCTCAGGG ATGAGTAGTT CCATCATGCA GCTGTATCTC
    AAACAGGTCC TTGAGGCTTT CTTTCATACC CAGTCCAATG TACGCCACTT TGCTCTAAAT
    GTCATTGCAC TGACGTTAAA CCAGGGTCTC ATCCATCCTG TTCAGTGTGT GCCATACTTA
    ATTGCTATGG GCACAGATCC AGAGCCTTCT ATGCGAAACA AAGCTGACCA GCAGTTGGTG
    GAAATAGACA AAAAGTATGC TGGTTTCATT CACATGAAAG CAGTGGCTGG TATGAAGATG
    TCTTACCAGG TACAACAGGC AATCAATACC TGCCCAAAGG ATCCTGTAAG AGGCTTTAGA
    CACGATGAAT CATCCAGTGC ATTGTGTTCA CACCTATATT CTATGATCCG AGGGAACCGT
    CAACACAGAC GAGCATTTCT AATTTCTTTA CTCAATCTCT TCGATGATAC TGCAAAAACA
    GAGGTGAATA TGCTCTTGTA CATAGCAGAC AATCTAGCCT GTTTTCCTTA TCAAACGCAG
    GAAGAGCCAC TGTTTATAAT GCATCATATA GACATTACAC TATCAGTTTC TGGTAGCAAC
    CTACTACAGT CATTTAAAGA GTCAATGGTG AAAGACAAAA AAAAGGAAAG AAAGCCTTCA
    TCAGATGAGG AGACTGAGAG TGACAGTAAT AGTGGCAGTG AGAGCGAAAG TGAAGAGGAA
    GCTTCTAAGC CTCGGAGGTT ACGAAAACGA GTAGATTCTG ACTCAGATTC TGATGAAGAA
    AATGATATAA ATGCTGTGAT GAAATGTTTA CCAGAAAACT CAGCTCCTTT AATTGAATTT
    GCAAATGTGT CTCAAGGCAT ATTATTACTT TTGATGCTGA AACAGCATTT AAAGAACCTT
    TGCGGATTCT CTGATAGTAA AATTCAGAAA TATTCTCCAT CTGAGTCTGC AAAAGTTTAT
    GATAAAGCGA TAAACAGGAA GACAGGAGTG CATTTCCATC CAAAACAAAC TCTGGATTTT
    CTACGGAGTG ATATGGCTAA TTCCAAGATC ACTGAAGAAG TGAAGAGGAG TATAGTAAAA
    CAGTACTTAG ATTTCAAGCT CCTTATGGAA CATTTGGATC CTGATGAAGA AGAAGAAGAT
    GGAGAGGTGT CAGCTAGCAC AAATGCTCGG AACAAAGCAA TTACCTCGCT GCTTGGAGGA
    GGCAGCCCTA AAAACAATGC AGCTGAGACA GAGGACGATG AAAGTGATGG GGAGGATAGA
    GGAGGAGGCA CTTCAGGGTC ATTGAGAAGG TCAAAACGAA ATTCAGACTC TACGGAGTTG
    GCTTCACAGA TGAATGAAAG TGTTGATGTC ATGGATGTCA TCGCTATTTG CTGTCCAAAG
    TACAAAGATC GACCACAAAT TGCAAGAGTA GTACAGAAAA CCAGCAATGG CTTCAGTGTT
    CAGTGGATGG CAGGCTCCTA CAGTGGCTCC TGGACTGAGG CTAAGCGCCG TGATGGCCGC
    AAACTGGTGC CTTGGGTAGA CACTATTAAA GAGTCAGACA TTATTTACAA AAAAATTGCT
    CTAACGAGTG CTAATAAGCT GACTAATAAA GTTGTTCAGA CTTTACGATC GCTGTATGCC
    GCCAAGGATG GAACTTCGAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004937252.1
    CDS3233..10036
    Translation

    Target ORF information:

    RefSeq Version XM_004937195.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004937195.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGGTAGCATA
    GATCAGTCAG TGCTAAAGGA GTTGCCTCCT GAGCTCCTGG CAGAAATTGA ATCCACCATG
    CCTCTTTGTG AACGCGTGAA AATGAACAAG CGCAAACGTA GCACAGTTAA TGAGAAACCA
    AAATATGCTG AAATCAGTTC TGATGAAGAC AATGACAGTG AAGAAGCTTT TGAATCTTCT
    CGAAAAAGAC ATAAAAAGGA TAGAGATGAA GCTTGGGAGT ACGAAGAGCA TGACAGAAGA
    AGTTCTGGTG ACCACAGGAG GAGTGGTCAT TATCATGAAG GAAGAAGGAC TTCTGGTAGT
    GGCCGTTACC GCAATCGCAG TCCCGATGAC TCTGATCTGG ATGATTATTC TCCTCCTCCT
    AGCCTCAGTG AGGTGGCAAG AAAAATGAAG AAAAAAGAAA AGCAAAAGAA GAGGAGAGCT
    TACGAGCCTA AACTAACACC TGAAGAAATG ATGGATTCTT CAACCTTCAA AAGATTTATT
    GCTTCAATAG AGAATATTCT GGAAAATCTG GAAGACATGG ATTTTACAAC CTTGGGTGAT
    GATGATGAGA TTCCACAGGA ATTGCTTTTG GGAAAACATC AGCTTAATGA GTTGGGTAGT
    GAATCTGCAA AAATCAAGGC AATGGGTATT ATGGACAAGC TCTCAACAGA TAAAATAGTA
    AAGGTCCTGA ACATTTTGGA GAAGAATATT CAAGACGGAT CAAAGCTTTC TACATTACTT
    AACCACAACA ATGACACTGA AGATGAGGAG AGATTATGGA GAGATCTCAT AATGGAGAGA
    GTAACAAAAT CTGCAGATGC TTGTCTTACT GCTATCAATA TTATGACATC ACCAAATATG
    CCTAAAGCTG TTTATATTGA GGACGTAATA GAAAGAGTTA TTCAGTACAC AAAATTTCAT
    TTGCAGAACA CTCTCTATCC TCAGTATGAC CCTGTGTATA GAGTGGATCC TCTTGGTGGT
    GGTCTTTTAA GTTCAAAAGC AAAACGTGCC AAGTGTTCCA CCCACAAACA AAGAGTTATA
    GTGATGTTGT ATAACAAAGT TTGTGATATT GTCAGCAGTT TGTCAGAGTT GCTAGAGATA
    CAACTTCTTA CTGATACAAC TATTCTTCAG GTATCGTCTA TGGGGATAAC ACCTTTCTTT
    GTAGAAAATG TCAGTGAACT ACAGCTATGT GCCATCAAAT TAGTGACTGC AGTCTTCTCC
    AGGTATGAAA AACATAGACA GCTAATACTA GAAGAAATTT TTACTTCCCT TGCAAGACTA
    CCAACTAGCA AGAGGAGCCT GAGAAACTTC AGGTTGAATA GCAGTGACAC AGATGGAGAG
    CCTATGTATA TTCAGATGGT AACAGCACTA GTTCTGCAGC TTATTCAATG TGTCGTGCAC
    TTACCATCAG CAGAAAAAGA TTCTAATTCA GATGAGGAAT TGAACAAAAA AGTGGATCAA
    GATGTGCTTA TTACTAACTC ATATGAAACA GCTATGAGAA CAGCACAAAA TTTTCTTTCT
    ATTTTTCTTA AGAAATGTGG TAGTAAACAA GGAGAAGAAG ATTACAGGCC TCTCTTTGAG
    AACTTCATTC AAGATCTTCT TTCCACAGTC AATAAACCAG AATGGCCAGC TGCAGAGTTG
    CTGCTTAGCT TACTAGGAAG GTTATTGGTT CACCAGTTCA GTAATAAATC AACAGAAATG
    GCTTTAAGAG TTGCGTCACT TGACTATCTT GGAACCGTAG CTGCAAGACT GAGGAAAGAT
    GCAGTCACTA GTAAAATGGA TCAAGGATCT ATAGCTAGAA TCCTCAAACA GGCCTCAGGA
    GGAGAAGATG ATATTCAACA GCTACAGAAG GCTTTATTGG ATTATCTGGA TGAGAACACT
    GAAACTGATG CATCGTTAGT GTTTTCTCGG AAGTTTTATA TAGCTCAGTG GTTTCGGGAT
    ACAACTATGG AGACAGAGAA GGCAATTAAA TCTCAGAAGG ATGAGGATTC ATCTGAAGGA
    ACTCATCATG CAAAAGATGT TGAGACCACA GGACAAATCA TGCACAGAGC AGAAAGCCGC
    AAGAAGTTTC TCCGCAGCAT CATTAAAACT GCTCCATCTC AGTTCAGTAC ATTAAAGATT
    TATTCTGACA CTGTGGACTA TGAAGATGCA TGTTTGATTG TTCGCTACTT GGCCTCTATG
    AGGCCGTTTG CCCAGAGCTT CGATATTTAT TTAACACAGA TTCTGCGTGT ACTTGGTGAA
    AATGCAATTG CAGTTCGAAC AAAAGCCATG AAGTGTTTGT CTGAGGTTGT TGCTGTAGAT
    CCCAGTATTC TAGCCAGGCC GGATATGCAG CGAGGTGTTC ATGGACGGTT AATGGATAAC
    TCCACCAGTG TACGGGAAGC AGCTGTGGAG CTGCTTGGCC GATTTGTGCT CTGTCGTCCT
    CAGCTTGCTG AGCAGTATTA TGACATGCTG ATTGAAAGAA TATTGGATAC TGGTATCAGT
    GTCAGAAAAA GAGTCATAAA GATACTTAGG GATATCTGCA TTGAACAACC AACGTTTCCC
    AAAATTACAG AGATGTGTGT GAAAATGATT CGGAGAGTCA ATGATGAAGA AGGCATTAAG
    AAATTAGTAA ATGAGACATT CCAGAAGCTG TGGTTTACTC CAACTCCACA CCATGACAAA
    GAAGCAATGA CTAGGAAAAT ACTGAACATC ACTGATGTGG TGGCAGCTTG TAGGGATACA
    GGGTATGATT GGTTTGAGCA GCTTCTTCAA AATCTATTGA AGTCAGAAGA GGATGCTTCA
    TATAAACCTG TGAAGAAGGC TTGTACTCAA CTTGTTGACA ATTTGGTTGA GCACATCCTT
    AAATATGAGG AGTCTTTATC AGACTCTGAC AACAAAGGAG TGAATTCTGG TCGCTTGGTT
    GCTTGTATAA CCACTCTGTT CTTATTCAGC AAAATCAGGC CCCAGTTAAT GGTCAAACAT
    GCCATGACCA TGCAGCCATA TCTGGCAACT AAATGTAGTA CTCAGAATGA TTTCATGGTG
    ATCTGCAATG TTGCAAAAAT CTTGGAGCTT GTAGTGCCGT TAATGGAACA CCCAAGTGAG
    ACCTTCCTTG CCACTATTGA GGAAGACCTC ATGAAACTCA TCATCAAATA TGGCATGACA
    GTTGTTCAAC ATTGTGTGAG CTGCCTTGGG TCTGTTGTGA ACAAGGTCAC CCAGAATTAC
    AAGTTTGTGT GGGCCTGTTT TAATAGATAC TATGGTGCAC TTTCAAAATT AAAAAGTCAA
    CACCAAGAAG ACCCCAACAG TACAATACTT ACTGCCAATA AACCAGCACT CCTTAGATCA
    CTTTTCACTG TTGGAGCACT GTGTCGGCAT TTTGATTTTG ACCATGAAGA TTTTAAGGGC
    AACAGTAAGG TTAACATTAA GGATAAAGTA CTTGAACTGC TGATGTATTT TACAAAGCAT
    TCAGACGAAG AAGTACAGAC AAAAGCCATT ATTGGCCTGG GATTTGCATT TATACAACAC
    CCAAGCTTAA TGTTTGAACA GGAAGTAAAG ACTCTGTACA ACAGCATTCT TTCAGATAAG
    AACTGTTCAG TAAACCTAAA AATCCAGGTG TTAAAAAACC TCCAGACCTA CTTGCAGGAG
    GAGGACACAC GTATGCAGCA GGCAGACAGA GACTGGAAAA AAGTAGCAAA ACAGGAAGAC
    CTGAAAGAAA TGGGTGATAT TTCCTCAGGG ATGAGTAGTT CCATCATGCA GCTGTATCTC
    AAACAGGTCC TTGAGGCTTT CTTTCATACC CAGTCCAATG TACGCCACTT TGCTCTAAAT
    GTCATTGCAC TGACGTTAAA CCAGGGTCTC ATCCATCCTG TTCAGTGTGT GCCATACTTA
    ATTGCTATGG GCACAGATCC AGAGCCTTCT ATGCGAAACA AAGCTGACCA GCAGTTGGTG
    GAAATAGACA AAAAGTATGC TGGTTTCATT CACATGAAAG CAGTGGCTGG TATGAAGATG
    TCTTACCAGG TACAACAGGC AATCAATACC TGCCCAAAGG ATCCTGTAAG AGGCTTTAGA
    CACGATGAAT CATCCAGTGC ATTGTGTTCA CACCTATATT CTATGATCCG AGGGAACCGT
    CAACACAGAC GAGCATTTCT AATTTCTTTA CTCAATCTCT TCGATGATAC TGCAAAAACA
    GAGGTGAATA TGCTCTTGTA CATAGCAGAC AATCTAGCCT GTTTTCCTTA TCAAACGCAG
    GAAGAGCCAC TGTTTATAAT GCATCATATA GACATTACAC TATCAGTTTC TGGTAGCAAC
    CTACTACAGT CATTTAAAGA GTCAATGGTG AAAGACAAAA AAAAGGAAAG AAAGCCTTCA
    TCAGATGAGG AGACTGAGAG TGACAGTAAT AGTGGCAGTG AGAGCGAAAG TGAAGAGGAA
    GCTTCTAAGC CTCGGAGGTT ACGAAAACGA GTAGATTCTG ACTCAGATTC TGATGAAGAA
    AATGATATAA ATGCTGTGAT GAAATGTTTA CCAGAAAACT CAGCTCCTTT AATTGAATTT
    GCAAATGTGT CTCAAGGCAT ATTATTACTT TTGATGCTGA AACAGCATTT AAAGAACCTT
    TGCGGATTCT CTGATAGTAA AATTCAGAAA TATTCTCCAT CTGAGTCTGC AAAAGTTTAT
    GATAAAGCGA TAAACAGGAA GACAGGAGTG CATTTCCATC CAAAACAAAC TCTGGATTTT
    CTACGGAGTG ATATGGCTAA TTCCAAGATC ACTGAAGAAG TGAAGAGGAG TATAGTAAAA
    CAGTACTTAG ATTTCAAGCT CCTTATGGAA CATTTGGATC CTGATGAAGA AGAAGAAGAT
    GGAGAGGTGT CAGCTAGCAC AAATGCTCGG AACAAAGCAA TTACCTCGCT GCTTGGAGGA
    GGCAGCCCTA AAAACAATGC AGCTGAGACA GAGGACGATG AAAGTGATGG GGAGGATAGA
    GGAGGAGGCA CTTCAGGGTC ATTGAGAAGG TCAAAACGAA ATTCAGACTC TACGGAGTTG
    GCTTCACAGA TGAATGAAAG TGTTGATGTC ATGGATGTCA TCGCTATTTG CTGTCCAAAG
    TACAAAGATC GACCACAAAT TGCAAGAGTA GTACAGAAAA CCAGCAATGG CTTCAGTGTT
    CAGTGGATGG CAGGCTCCTA CAGTGGCTCC TGGACTGAGG CTAAGCGCCG TGATGGCCGC
    AAACTGGTGC CTTGGGTAGA CACTATTAAA GAGTCAGACA TTATTTACAA AAAAATTGCT
    CTAACGAGTG CTAATAAGCT GACTAATAAA GTTGTTCAGA CTTTACGATC GCTGTATGCC
    GCCAAGGATG GAACTTCGAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa20946
    Clone ID Related Accession (Same CDS sequence) XM_425012.5 , XM_425012.6 , XM_015277519.2 , XM_015277519.1 , XM_015277520.1 , XM_015277520.2
    Accession Version XM_425012.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8352bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product nipped-B-like protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_425012.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGATAAGCCT
    CTGAAGAAGC GAAAACAAGA AAACAAACCA CAGGAGGCTG GGGGTGCTGC TGGAGGAAAT
    AGGCCAGCTT CTCAGGAGTC AGGTTCTGCA GGAAATGGGG CAAGGCCAGC ACTGATGGTT
    AGTATTGATC TTCAACAAGC AGGAAGAGCG GACTCTCAGG CAACAATAAC TCAGGAATTT
    GAGGCTATCA AAAAACCTGA AGAGATTAAG CAGTATAATG ATGGATTTGT GTGTATTCCC
    CAAGAAGAGA CTGTTGGAGT TCTTAAACTT AAACCTGAAA ACCACCCTGA GATTTTTAGG
    AAAAAGTCAG ACTTTGAGGT TCAAAAGACA GATATCCAGC AAAATGAAAA CAGACAAATG
    GAGTTTCAGC AAAATGAGAG CAGAAGGTTG GAAAGTAAAT ACAATGACAA TAAACAGGTA
    GAAGCTAAAT ATGACATCAA GCATGACAGT AGACAAATGG AGATGAAACA AAATGAGAAC
    AGACAGGCAG AGTCAAAACA AAATGAGAGC AGACAAATGG AGATGAAACA AAATGAGGGA
    AAACAAAATA ATGGCAAACA AGAAATAAGA CAGAGACCTG AAACACCAAA GCAGAAGAGC
    GAAGGCAGGC CTGAAACACC CAAGCAGAAG AGTGAAGGCA GGCCTGAAAC ACCAAAACAT
    AGGCATGATA ACAGGAGGGA CTCCAGTAAA CCATTGTCAG AAAAGAAAAT TGAATTATCT
    AGGCATAAAC CTGATGTGAA ATCTGATACT TCAAAGGTAA AATCTGATAG TAGATCAGAT
    CCCGTGAAGC AAAGGCCTGA TGGGCGTTCA GTTTCTGATA CTCAGAGGCG TGACCACGAT
    AGCCTTAAAC AGAGATCTGA GGACAGGGAA TCAGAAAGAT ACAAAGGAGA TCCATCCAAG
    ATTAGAAGGA CTGAATTTTT GAGATCCTCA AACAGAAATG AACATGATTC TAAGTATGGT
    ATTAAATCTG ATGGTTCAAA ACCTGAAAAA TTTGAAAGGA AACACAGACA TGACTCTGGT
    GAATCAAGGG AAAGATTTTC TTCTGGGGAT CAGAAGTCAG ATCAAAAGTC AAGACCTGAC
    AGCCCTCGAG TTAAACAGGA TGGCAAAGGA GATTCTAGCA AATCAAGACC GGATAAACCT
    GGTTTTAGAT CACCCAACAG CAAGGATGAA CGAAGGACAG ACAGTAGTAA GAGTAAAGTA
    GAGAGTAATA AAGCACATCC TGACAATAAA GCAGAGTTTC CCAGTTATCT GCTGGGGGCA
    AGATCTGGTG CACTGAAACA TTTTGTCATT CCAAAAATCA AACGTGACAA AGATGGCAAT
    GTCACTCAGG AGTCAAGGAA AACTGAACTT AAAGGTGAAC AGAAGGACAA AGTAGAGAAG
    ATTGGGCTAG TTGAAGACCT AAATAAAGGA GCTAAGCCTG TAGTTGTCCT TCAGAAGCTT
    TCTTTGGACG ATGTACAAAA ATTTATTAAG GATAGAGAAG ATAAATCAAG GGGCTCTTGT
    TTTAAACCTA ACAAGAACAA GTCGTCCAAA TCTAATAAAG GTAGCATAGA TCAGTCAGTG
    CTAAAGGAGT TGCCTCCTGA GCTCCTGGCA GAAATTGAAT CCACCATGCC TCTTTGTGAA
    CGCGTGAAAA TGAACAAGCG CAAACGTAGC ACAGTTAATG AGAAACCAAA ATATGCTGAA
    ATCAGTTCTG ATGAAGACAA TGACAGTGAA GAAGCTTTTG AATCTTCTCG AAAAAGACAT
    AAAAAGGATA GAGATGAAGC TTGGGAGTAC GAAGAGCATG ACAGAAGAAG TTCTGGTGAC
    CACAGGAGGA GTGGTCATTA TCATGAAGGA AGAAGGACTT CTGGTAGTGG CCGTTACCGC
    AATCGCAGTC CCGATGACTC TGATCTGGAT GATTATTCTC CTCCTCCTAG CCTCAGTGAG
    GTGGCAAGAA AAATGAAGAA AAAAGAAAAG CAAAAGAAGA GGAGAGCTTA CGAGCCTAAA
    CTAACACCTG AAGAAATGAT GGATTCTTCA ACCTTCAAAA GATTTATTGC TTCAATAGAG
    AATATTCTGG AAAATCTGGA AGACATGGAT TTTACAACCT TGGGTGATGA TGATGAGATT
    CCACAGGAAT TGCTTTTGGG AAAACATCAG CTTAATGAGT TGGGTAGTGA ATCTGCAAAA
    ATCAAGGCAA TGGGTATTAT GGACAAGCTC TCAACAGATA AAATAGTAAA GGTCCTGAAC
    ATTTTGGAGA AGAATATTCA AGACGGATCA AAGCTTTCTA CATTACTTAA CCACAACAAT
    GACACTGAAG ATGAGGAGAG ATTATGGAGA GATCTCATAA TGGAGAGAGT AACAAAATCT
    GCAGATGCTT GTCTTACTGC TATCAATATT ATGACATCAC CAAATATGCC TAAAGCTGTT
    TATATTGAGG ACGTAATAGA AAGAGTTATT CAGTACACAA AATTTCATTT GCAGAACACT
    CTCTATCCTC AGTATGACCC TGTGTATAGA GTGGATCCTC TTGGTGGTGG TCTTTTAAGT
    TCAAAAGCAA AACGTGCCAA GTGTTCCACC CACAAACAAA GAGTTATAGT GATGTTGTAT
    AACAAAGTTT GTGATATTGT CAGCAGTTTG TCAGAGTTGC TAGAGATACA ACTTCTTACT
    GATACAACTA TTCTTCAGGT ATCGTCTATG GGGATAACAC CTTTCTTTGT AGAAAATGTC
    AGTGAACTAC AGCTATGTGC CATCAAATTA GTGACTGCAG TCTTCTCCAG GTATGAAAAA
    CATAGACAGC TAATACTAGA AGAAATTTTT ACTTCCCTTG CAAGACTACC AACTAGCAAG
    AGGAGCCTGA GAAACTTCAG GTTGAATAGC AGTGACACAG ATGGAGAGCC TATGTATATT
    CAGATGGTAA CAGCACTAGT TCTGCAGCTT ATTCAATGTG TCGTGCACTT ACCATCAGCA
    GAAAAAGATT CTAATTCAGA TGAGGAATTG AACAAAAAAG TGGATCAAGA TGTGCTTATT
    ACTAACTCAT ATGAAACAGC TATGAGAACA GCACAAAATT TTCTTTCTAT TTTTCTTAAG
    AAATGTGGTA GTAAACAAGG AGAAGAAGAT TACAGGCCTC TCTTTGAGAA CTTCATTCAA
    GATCTTCTTT CCACAGTCAA TAAACCAGAA TGGCCAGCTG CAGAGTTGCT GCTTAGCTTA
    CTAGGAAGGT TATTGGTTCA CCAGTTCAGT AATAAATCAA CAGAAATGGC TTTAAGAGTT
    GCGTCACTTG ACTATCTTGG AACCGTAGCT GCAAGACTGA GGAAAGATGC AGTCACTAGT
    AAAATGGATC AAGGATCTAT AGCTAGAATC CTCAAACAGG CCTCAGGAGG AGAAGATGAT
    ATTCAACAGC TACAGAAGGC TTTATTGGAT TATCTGGATG AGAACACTGA AACTGATGCA
    TCGTTAGTGT TTTCTCGGAA GTTTTATATA GCTCAGTGGT TTCGGGATAC AACTATGGAG
    ACAGAGAAGG CAATTAAATC TCAGAAGGAT GAGGATTCAT CTGAAGGAAC TCATCATGCA
    AAAGATGTTG AGACCACAGG ACAAATCATG CACAGAGCAG AAAGCCGCAA GAAGTTTCTC
    CGCAGCATCA TTAAAACTGC TCCATCTCAG TTCAGTACAT TAAAGATTTA TTCTGACACT
    GTGGACTATG AAGATGCATG TTTGATTGTT CGCTACTTGG CCTCTATGAG GCCGTTTGCC
    CAGAGCTTCG ATATTTATTT AACACAGATT CTGCGTGTAC TTGGTGAAAA TGCAATTGCA
    GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGATCC CAGTATTCTA
    GCCAGGCCGG ATATGCAGCG AGGTGTTCAT GGACGGTTAA TGGATAACTC CACCAGTGTA
    CGGGAAGCAG CTGTGGAGCT GCTTGGCCGA TTTGTGCTCT GTCGTCCTCA GCTTGCTGAG
    CAGTATTATG ACATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGAAAAAGA
    GTCATAAAGA TACTTAGGGA TATCTGCATT GAACAACCAA CGTTTCCCAA AATTACAGAG
    ATGTGTGTGA AAATGATTCG GAGAGTCAAT GATGAAGAAG GCATTAAGAA ATTAGTAAAT
    GAGACATTCC AGAAGCTGTG GTTTACTCCA ACTCCACACC ATGACAAAGA AGCAATGACT
    AGGAAAATAC TGAACATCAC TGATGTGGTG GCAGCTTGTA GGGATACAGG GTATGATTGG
    TTTGAGCAGC TTCTTCAAAA TCTATTGAAG TCAGAAGAGG ATGCTTCATA TAAACCTGTG
    AAGAAGGCTT GTACTCAACT TGTTGACAAT TTGGTTGAGC ACATCCTTAA ATATGAGGAG
    TCTTTATCAG ACTCTGACAA CAAAGGAGTG AATTCTGGTC GCTTGGTTGC TTGTATAACC
    ACTCTGTTCT TATTCAGCAA AATCAGGCCC CAGTTAATGG TCAAACATGC CATGACCATG
    CAGCCATATC TGGCAACTAA ATGTAGTACT CAGAATGATT TCATGGTGAT CTGCAATGTT
    GCAAAAATCT TGGAGCTTGT AGTGCCGTTA ATGGAACACC CAAGTGAGAC CTTCCTTGCC
    ACTATTGAGG AAGACCTCAT GAAACTCATC ATCAAATATG GCATGACAGT TGTTCAACAT
    TGTGTGAGCT GCCTTGGGTC TGTTGTGAAC AAGGTCACCC AGAATTACAA GTTTGTGTGG
    GCCTGTTTTA ATAGATACTA TGGTGCACTT TCAAAATTAA AAAGTCAACA CCAAGAAGAC
    CCCAACAGTA CAATACTTAC TGCCAATAAA CCAGCACTCC TTAGATCACT TTTCACTGTT
    GGAGCACTGT GTCGGCATTT TGATTTTGAC CATGAAGATT TTAAGGGCAA CAGTAAGGTT
    AACATTAAGG ATAAAGTACT TGAACTGCTG ATGTATTTTA CAAAGCATTC AGACGAAGAA
    GTACAGACAA AAGCCATTAT TGGCCTGGGA TTTGCATTTA TACAACACCC AAGCTTAATG
    TTTGAACAGG AAGTAAAGAC TCTGTACAAC AGCATTCTTT CAGATAAGAA CTGTTCAGTA
    AACCTAAAAA TCCAGGTGTT AAAAAACCTC CAGACCTACT TGCAGGAGGA GGACACACGT
    ATGCAGCAGG CAGACAGAGA CTGGAAAAAA GTAGCAAAAC AGGAAGACCT GAAAGAAATG
    GGTGATATTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TGTATCTCAA ACAGGTCCTT
    GAGGCTTTCT TTCATACCCA GTCCAATGTA CGCCACTTTG CTCTAAATGT CATTGCACTG
    ACGTTAAACC AGGGTCTCAT CCATCCTGTT CAGTGTGTGC CATACTTAAT TGCTATGGGC
    ACAGATCCAG AGCCTTCTAT GCGAAACAAA GCTGACCAGC AGTTGGTGGA AATAGACAAA
    AAGTATGCTG GTTTCATTCA CATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
    CAACAGGCAA TCAATACCTG CCCAAAGGAT CCTGTAAGAG GCTTTAGACA CGATGAATCA
    TCCAGTGCAT TGTGTTCACA CCTATATTCT ATGATCCGAG GGAACCGTCA ACACAGACGA
    GCATTTCTAA TTTCTTTACT CAATCTCTTC GATGATACTG CAAAAACAGA GGTGAATATG
    CTCTTGTACA TAGCAGACAA TCTAGCCTGT TTTCCTTATC AAACGCAGGA AGAGCCACTG
    TTTATAATGC ATCATATAGA CATTACACTA TCAGTTTCTG GTAGCAACCT ACTACAGTCA
    TTTAAAGAGT CAATGGTGAA AGACAAAAAA AAGGAAAGAA AGCCTTCATC AGATGAGGAG
    ACTGAGAGTG ACAGTAATAG TGGCAGTGAG AGCGAAAGTG AAGAGGAAGC TTCTAAGCCT
    CGGAGGTTAC GAAAACGAGT AGATTCTGAC TCAGATTCTG ATGAAGAAAA TGATATAAAT
    GCTGTGATGA AATGTTTACC AGAAAACTCA GCTCCTTTAA TTGAATTTGC AAATGTGTCT
    CAAGGCATAT TATTACTTTT GATGCTGAAA CAGCATTTAA AGAACCTTTG CGGATTCTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCT GAGTCTGCAA AAGTTTATGA TAAAGCGATA
    AACAGGAAGA CAGGAGTGCA TTTCCATCCA AAACAAACTC TGGATTTTCT ACGGAGTGAT
    ATGGCTAATT CCAAGATCAC TGAAGAAGTG AAGAGGAGTA TAGTAAAACA GTACTTAGAT
    TTCAAGCTCC TTATGGAACA TTTGGATCCT GATGAAGAAG AAGAAGATGG AGAGGTGTCA
    GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AACAATGCAG CTGAGACAGA GGACGATGAA AGTGATGGGG AGGATAGAGG AGGAGGCACT
    TCAGGGTCAT TGAGAAGGTC AAAACGAAAT TCAGACTCTA CGGAGTTGGC TTCACAGATG
    AATGAAAGTG TTGATGTCAT GGATGTCATC GCTATTTGCT GTCCAAAGTA CAAAGATCGA
    CCACAAATTG CAAGAGTAGT ACAGAAAACC AGCAATGGCT TCAGTGTTCA GTGGATGGCA
    GGCTCCTACA GTGGCTCCTG GACTGAGGCT AAGCGCCGTG ATGGCCGCAA ACTGGTGCCT
    TGGGTAGACA CTATTAAAGA GTCAGACATT ATTTACAAAA AAATTGCTCT AACGAGTGCT
    AATAAGCTGA CTAATAAAGT TGTTCAGACT TTACGATCGC TGTATGCCGC CAAGGATGGA
    ACTTCGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_425012.3
    CDS524..8875
    Translation

    Target ORF information:

    RefSeq Version XM_425012.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_425012.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGATAAGCCT
    CTGAAGAAGC GAAAACAAGA AAACAAACCA CAGGAGGCTG GGGGTGCTGC TGGAGGAAAT
    AGGCCAGCTT CTCAGGAGTC AGGTTCTGCA GGAAATGGGG CAAGGCCAGC ACTGATGGTT
    AGTATTGATC TTCAACAAGC AGGAAGAGCG GACTCTCAGG CAACAATAAC TCAGGAATTT
    GAGGCTATCA AAAAACCTGA AGAGATTAAG CAGTATAATG ATGGATTTGT GTGTATTCCC
    CAAGAAGAGA CTGTTGGAGT TCTTAAACTT AAACCTGAAA ACCACCCTGA GATTTTTAGG
    AAAAAGTCAG ACTTTGAGGT TCAAAAGACA GATATCCAGC AAAATGAAAA CAGACAAATG
    GAGTTTCAGC AAAATGAGAG CAGAAGGTTG GAAAGTAAAT ACAATGACAA TAAACAGGTA
    GAAGCTAAAT ATGACATCAA GCATGACAGT AGACAAATGG AGATGAAACA AAATGAGAAC
    AGACAGGCAG AGTCAAAACA AAATGAGAGC AGACAAATGG AGATGAAACA AAATGAGGGA
    AAACAAAATA ATGGCAAACA AGAAATAAGA CAGAGACCTG AAACACCAAA GCAGAAGAGC
    GAAGGCAGGC CTGAAACACC CAAGCAGAAG AGTGAAGGCA GGCCTGAAAC ACCAAAACAT
    AGGCATGATA ACAGGAGGGA CTCCAGTAAA CCATTGTCAG AAAAGAAAAT TGAATTATCT
    AGGCATAAAC CTGATGTGAA ATCTGATACT TCAAAGGTAA AATCTGATAG TAGATCAGAT
    CCCGTGAAGC AAAGGCCTGA TGGGCGTTCA GTTTCTGATA CTCAGAGGCG TGACCACGAT
    AGCCTTAAAC AGAGATCTGA GGACAGGGAA TCAGAAAGAT ACAAAGGAGA TCCATCCAAG
    ATTAGAAGGA CTGAATTTTT GAGATCCTCA AACAGAAATG AACATGATTC TAAGTATGGT
    ATTAAATCTG ATGGTTCAAA ACCTGAAAAA TTTGAAAGGA AACACAGACA TGACTCTGGT
    GAATCAAGGG AAAGATTTTC TTCTGGGGAT CAGAAGTCAG ATCAAAAGTC AAGACCTGAC
    AGCCCTCGAG TTAAACAGGA TGGCAAAGGA GATTCTAGCA AATCAAGACC GGATAAACCT
    GGTTTTAGAT CACCCAACAG CAAGGATGAA CGAAGGACAG ACAGTAGTAA GAGTAAAGTA
    GAGAGTAATA AAGCACATCC TGACAATAAA GCAGAGTTTC CCAGTTATCT GCTGGGGGCA
    AGATCTGGTG CACTGAAACA TTTTGTCATT CCAAAAATCA AACGTGACAA AGATGGCAAT
    GTCACTCAGG AGTCAAGGAA AACTGAACTT AAAGGTGAAC AGAAGGACAA AGTAGAGAAG
    ATTGGGCTAG TTGAAGACCT AAATAAAGGA GCTAAGCCTG TAGTTGTCCT TCAGAAGCTT
    TCTTTGGACG ATGTACAAAA ATTTATTAAG GATAGAGAAG ATAAATCAAG GGGCTCTTGT
    TTTAAACCTA ACAAGAACAA GTCGTCCAAA TCTAATAAAG GTAGCATAGA TCAGTCAGTG
    CTAAAGGAGT TGCCTCCTGA GCTCCTGGCA GAAATTGAAT CCACCATGCC TCTTTGTGAA
    CGCGTGAAAA TGAACAAGCG CAAACGTAGC ACAGTTAATG AGAAACCAAA ATATGCTGAA
    ATCAGTTCTG ATGAAGACAA TGACAGTGAA GAAGCTTTTG AATCTTCTCG AAAAAGACAT
    AAAAAGGATA GAGATGAAGC TTGGGAGTAC GAAGAGCATG ACAGAAGAAG TTCTGGTGAC
    CACAGGAGGA GTGGTCATTA TCATGAAGGA AGAAGGACTT CTGGTAGTGG CCGTTACCGC
    AATCGCAGTC CCGATGACTC TGATCTGGAT GATTATTCTC CTCCTCCTAG CCTCAGTGAG
    GTGGCAAGAA AAATGAAGAA AAAAGAAAAG CAAAAGAAGA GGAGAGCTTA CGAGCCTAAA
    CTAACACCTG AAGAAATGAT GGATTCTTCA ACCTTCAAAA GATTTATTGC TTCAATAGAG
    AATATTCTGG AAAATCTGGA AGACATGGAT TTTACAACCT TGGGTGATGA TGATGAGATT
    CCACAGGAAT TGCTTTTGGG AAAACATCAG CTTAATGAGT TGGGTAGTGA ATCTGCAAAA
    ATCAAGGCAA TGGGTATTAT GGACAAGCTC TCAACAGATA AAATAGTAAA GGTCCTGAAC
    ATTTTGGAGA AGAATATTCA AGACGGATCA AAGCTTTCTA CATTACTTAA CCACAACAAT
    GACACTGAAG ATGAGGAGAG ATTATGGAGA GATCTCATAA TGGAGAGAGT AACAAAATCT
    GCAGATGCTT GTCTTACTGC TATCAATATT ATGACATCAC CAAATATGCC TAAAGCTGTT
    TATATTGAGG ACGTAATAGA AAGAGTTATT CAGTACACAA AATTTCATTT GCAGAACACT
    CTCTATCCTC AGTATGACCC TGTGTATAGA GTGGATCCTC TTGGTGGTGG TCTTTTAAGT
    TCAAAAGCAA AACGTGCCAA GTGTTCCACC CACAAACAAA GAGTTATAGT GATGTTGTAT
    AACAAAGTTT GTGATATTGT CAGCAGTTTG TCAGAGTTGC TAGAGATACA ACTTCTTACT
    GATACAACTA TTCTTCAGGT ATCGTCTATG GGGATAACAC CTTTCTTTGT AGAAAATGTC
    AGTGAACTAC AGCTATGTGC CATCAAATTA GTGACTGCAG TCTTCTCCAG GTATGAAAAA
    CATAGACAGC TAATACTAGA AGAAATTTTT ACTTCCCTTG CAAGACTACC AACTAGCAAG
    AGGAGCCTGA GAAACTTCAG GTTGAATAGC AGTGACACAG ATGGAGAGCC TATGTATATT
    CAGATGGTAA CAGCACTAGT TCTGCAGCTT ATTCAATGTG TCGTGCACTT ACCATCAGCA
    GAAAAAGATT CTAATTCAGA TGAGGAATTG AACAAAAAAG TGGATCAAGA TGTGCTTATT
    ACTAACTCAT ATGAAACAGC TATGAGAACA GCACAAAATT TTCTTTCTAT TTTTCTTAAG
    AAATGTGGTA GTAAACAAGG AGAAGAAGAT TACAGGCCTC TCTTTGAGAA CTTCATTCAA
    GATCTTCTTT CCACAGTCAA TAAACCAGAA TGGCCAGCTG CAGAGTTGCT GCTTAGCTTA
    CTAGGAAGGT TATTGGTTCA CCAGTTCAGT AATAAATCAA CAGAAATGGC TTTAAGAGTT
    GCGTCACTTG ACTATCTTGG AACCGTAGCT GCAAGACTGA GGAAAGATGC AGTCACTAGT
    AAAATGGATC AAGGATCTAT AGCTAGAATC CTCAAACAGG CCTCAGGAGG AGAAGATGAT
    ATTCAACAGC TACAGAAGGC TTTATTGGAT TATCTGGATG AGAACACTGA AACTGATGCA
    TCGTTAGTGT TTTCTCGGAA GTTTTATATA GCTCAGTGGT TTCGGGATAC AACTATGGAG
    ACAGAGAAGG CAATTAAATC TCAGAAGGAT GAGGATTCAT CTGAAGGAAC TCATCATGCA
    AAAGATGTTG AGACCACAGG ACAAATCATG CACAGAGCAG AAAGCCGCAA GAAGTTTCTC
    CGCAGCATCA TTAAAACTGC TCCATCTCAG TTCAGTACAT TAAAGATTTA TTCTGACACT
    GTGGACTATG AAGATGCATG TTTGATTGTT CGCTACTTGG CCTCTATGAG GCCGTTTGCC
    CAGAGCTTCG ATATTTATTT AACACAGATT CTGCGTGTAC TTGGTGAAAA TGCAATTGCA
    GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGATCC CAGTATTCTA
    GCCAGGCCGG ATATGCAGCG AGGTGTTCAT GGACGGTTAA TGGATAACTC CACCAGTGTA
    CGGGAAGCAG CTGTGGAGCT GCTTGGCCGA TTTGTGCTCT GTCGTCCTCA GCTTGCTGAG
    CAGTATTATG ACATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGAAAAAGA
    GTCATAAAGA TACTTAGGGA TATCTGCATT GAACAACCAA CGTTTCCCAA AATTACAGAG
    ATGTGTGTGA AAATGATTCG GAGAGTCAAT GATGAAGAAG GCATTAAGAA ATTAGTAAAT
    GAGACATTCC AGAAGCTGTG GTTTACTCCA ACTCCACACC ATGACAAAGA AGCAATGACT
    AGGAAAATAC TGAACATCAC TGATGTGGTG GCAGCTTGTA GGGATACAGG GTATGATTGG
    TTTGAGCAGC TTCTTCAAAA TCTATTGAAG TCAGAAGAGG ATGCTTCATA TAAACCTGTG
    AAGAAGGCTT GTACTCAACT TGTTGACAAT TTGGTTGAGC ACATCCTTAA ATATGAGGAG
    TCTTTATCAG ACTCTGACAA CAAAGGAGTG AATTCTGGTC GCTTGGTTGC TTGTATAACC
    ACTCTGTTCT TATTCAGCAA AATCAGGCCC CAGTTAATGG TCAAACATGC CATGACCATG
    CAGCCATATC TGGCAACTAA ATGTAGTACT CAGAATGATT TCATGGTGAT CTGCAATGTT
    GCAAAAATCT TGGAGCTTGT AGTGCCGTTA ATGGAACACC CAAGTGAGAC CTTCCTTGCC
    ACTATTGAGG AAGACCTCAT GAAACTCATC ATCAAATATG GCATGACAGT TGTTCAACAT
    TGTGTGAGCT GCCTTGGGTC TGTTGTGAAC AAGGTCACCC AGAATTACAA GTTTGTGTGG
    GCCTGTTTTA ATAGATACTA TGGTGCACTT TCAAAATTAA AAAGTCAACA CCAAGAAGAC
    CCCAACAGTA CAATACTTAC TGCCAATAAA CCAGCACTCC TTAGATCACT TTTCACTGTT
    GGAGCACTGT GTCGGCATTT TGATTTTGAC CATGAAGATT TTAAGGGCAA CAGTAAGGTT
    AACATTAAGG ATAAAGTACT TGAACTGCTG ATGTATTTTA CAAAGCATTC AGACGAAGAA
    GTACAGACAA AAGCCATTAT TGGCCTGGGA TTTGCATTTA TACAACACCC AAGCTTAATG
    TTTGAACAGG AAGTAAAGAC TCTGTACAAC AGCATTCTTT CAGATAAGAA CTGTTCAGTA
    AACCTAAAAA TCCAGGTGTT AAAAAACCTC CAGACCTACT TGCAGGAGGA GGACACACGT
    ATGCAGCAGG CAGACAGAGA CTGGAAAAAA GTAGCAAAAC AGGAAGACCT GAAAGAAATG
    GGTGATATTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TGTATCTCAA ACAGGTCCTT
    GAGGCTTTCT TTCATACCCA GTCCAATGTA CGCCACTTTG CTCTAAATGT CATTGCACTG
    ACGTTAAACC AGGGTCTCAT CCATCCTGTT CAGTGTGTGC CATACTTAAT TGCTATGGGC
    ACAGATCCAG AGCCTTCTAT GCGAAACAAA GCTGACCAGC AGTTGGTGGA AATAGACAAA
    AAGTATGCTG GTTTCATTCA CATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
    CAACAGGCAA TCAATACCTG CCCAAAGGAT CCTGTAAGAG GCTTTAGACA CGATGAATCA
    TCCAGTGCAT TGTGTTCACA CCTATATTCT ATGATCCGAG GGAACCGTCA ACACAGACGA
    GCATTTCTAA TTTCTTTACT CAATCTCTTC GATGATACTG CAAAAACAGA GGTGAATATG
    CTCTTGTACA TAGCAGACAA TCTAGCCTGT TTTCCTTATC AAACGCAGGA AGAGCCACTG
    TTTATAATGC ATCATATAGA CATTACACTA TCAGTTTCTG GTAGCAACCT ACTACAGTCA
    TTTAAAGAGT CAATGGTGAA AGACAAAAAA AAGGAAAGAA AGCCTTCATC AGATGAGGAG
    ACTGAGAGTG ACAGTAATAG TGGCAGTGAG AGCGAAAGTG AAGAGGAAGC TTCTAAGCCT
    CGGAGGTTAC GAAAACGAGT AGATTCTGAC TCAGATTCTG ATGAAGAAAA TGATATAAAT
    GCTGTGATGA AATGTTTACC AGAAAACTCA GCTCCTTTAA TTGAATTTGC AAATGTGTCT
    CAAGGCATAT TATTACTTTT GATGCTGAAA CAGCATTTAA AGAACCTTTG CGGATTCTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCT GAGTCTGCAA AAGTTTATGA TAAAGCGATA
    AACAGGAAGA CAGGAGTGCA TTTCCATCCA AAACAAACTC TGGATTTTCT ACGGAGTGAT
    ATGGCTAATT CCAAGATCAC TGAAGAAGTG AAGAGGAGTA TAGTAAAACA GTACTTAGAT
    TTCAAGCTCC TTATGGAACA TTTGGATCCT GATGAAGAAG AAGAAGATGG AGAGGTGTCA
    GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AACAATGCAG CTGAGACAGA GGACGATGAA AGTGATGGGG AGGATAGAGG AGGAGGCACT
    TCAGGGTCAT TGAGAAGGTC AAAACGAAAT TCAGACTCTA CGGAGTTGGC TTCACAGATG
    AATGAAAGTG TTGATGTCAT GGATGTCATC GCTATTTGCT GTCCAAAGTA CAAAGATCGA
    CCACAAATTG CAAGAGTAGT ACAGAAAACC AGCAATGGCT TCAGTGTTCA GTGGATGGCA
    GGCTCCTACA GTGGCTCCTG GACTGAGGCT AAGCGCCGTG ATGGCCGCAA ACTGGTGCCT
    TGGGTAGACA CTATTAAAGA GTCAGACATT ATTTACAAAA AAATTGCTCT AACGAGTGCT
    AATAAGCTGA CTAATAAAGT TGTTCAGACT TTACGATCGC TGTATGCCGC CAAGGATGGA
    ACTTCGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa20946
    Clone ID Related Accession (Same CDS sequence) XM_425012.5 , XM_425012.6 , XM_015277519.2 , XM_015277519.1 , XM_015277520.1 , XM_015277520.2
    Accession Version XM_425012.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8352bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product nipped-B-like protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_425012.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGATAAGCCT
    CTGAAGAAGC GAAAACAAGA AAACAAACCA CAGGAGGCTG GGGGTGCTGC TGGAGGAAAT
    AGGCCAGCTT CTCAGGAGTC AGGTTCTGCA GGAAATGGGG CAAGGCCAGC ACTGATGGTT
    AGTATTGATC TTCAACAAGC AGGAAGAGCG GACTCTCAGG CAACAATAAC TCAGGAATTT
    GAGGCTATCA AAAAACCTGA AGAGATTAAG CAGTATAATG ATGGATTTGT GTGTATTCCC
    CAAGAAGAGA CTGTTGGAGT TCTTAAACTT AAACCTGAAA ACCACCCTGA GATTTTTAGG
    AAAAAGTCAG ACTTTGAGGT TCAAAAGACA GATATCCAGC AAAATGAAAA CAGACAAATG
    GAGTTTCAGC AAAATGAGAG CAGAAGGTTG GAAAGTAAAT ACAATGACAA TAAACAGGTA
    GAAGCTAAAT ATGACATCAA GCATGACAGT AGACAAATGG AGATGAAACA AAATGAGAAC
    AGACAGGCAG AGTCAAAACA AAATGAGAGC AGACAAATGG AGATGAAACA AAATGAGGGA
    AAACAAAATA ATGGCAAACA AGAAATAAGA CAGAGACCTG AAACACCAAA GCAGAAGAGC
    GAAGGCAGGC CTGAAACACC CAAGCAGAAG AGTGAAGGCA GGCCTGAAAC ACCAAAACAT
    AGGCATGATA ACAGGAGGGA CTCCAGTAAA CCATTGTCAG AAAAGAAAAT TGAATTATCT
    AGGCATAAAC CTGATGTGAA ATCTGATACT TCAAAGGTAA AATCTGATAG TAGATCAGAT
    CCCGTGAAGC AAAGGCCTGA TGGGCGTTCA GTTTCTGATA CTCAGAGGCG TGACCACGAT
    AGCCTTAAAC AGAGATCTGA GGACAGGGAA TCAGAAAGAT ACAAAGGAGA TCCATCCAAG
    ATTAGAAGGA CTGAATTTTT GAGATCCTCA AACAGAAATG AACATGATTC TAAGTATGGT
    ATTAAATCTG ATGGTTCAAA ACCTGAAAAA TTTGAAAGGA AACACAGACA TGACTCTGGT
    GAATCAAGGG AAAGATTTTC TTCTGGGGAT CAGAAGTCAG ATCAAAAGTC AAGACCTGAC
    AGCCCTCGAG TTAAACAGGA TGGCAAAGGA GATTCTAGCA AATCAAGACC GGATAAACCT
    GGTTTTAGAT CACCCAACAG CAAGGATGAA CGAAGGACAG ACAGTAGTAA GAGTAAAGTA
    GAGAGTAATA AAGCACATCC TGACAATAAA GCAGAGTTTC CCAGTTATCT GCTGGGGGCA
    AGATCTGGTG CACTGAAACA TTTTGTCATT CCAAAAATCA AACGTGACAA AGATGGCAAT
    GTCACTCAGG AGTCAAGGAA AACTGAACTT AAAGGTGAAC AGAAGGACAA AGTAGAGAAG
    ATTGGGCTAG TTGAAGACCT AAATAAAGGA GCTAAGCCTG TAGTTGTCCT TCAGAAGCTT
    TCTTTGGACG ATGTACAAAA ATTTATTAAG GATAGAGAAG ATAAATCAAG GGGCTCTTGT
    TTTAAACCTA ACAAGAACAA GTCGTCCAAA TCTAATAAAG GTAGCATAGA TCAGTCAGTG
    CTAAAGGAGT TGCCTCCTGA GCTCCTGGCA GAAATTGAAT CCACCATGCC TCTTTGTGAA
    CGCGTGAAAA TGAACAAGCG CAAACGTAGC ACAGTTAATG AGAAACCAAA ATATGCTGAA
    ATCAGTTCTG ATGAAGACAA TGACAGTGAA GAAGCTTTTG AATCTTCTCG AAAAAGACAT
    AAAAAGGATA GAGATGAAGC TTGGGAGTAC GAAGAGCATG ACAGAAGAAG TTCTGGTGAC
    CACAGGAGGA GTGGTCATTA TCATGAAGGA AGAAGGACTT CTGGTAGTGG CCGTTACCGC
    AATCGCAGTC CCGATGACTC TGATCTGGAT GATTATTCTC CTCCTCCTAG CCTCAGTGAG
    GTGGCAAGAA AAATGAAGAA AAAAGAAAAG CAAAAGAAGA GGAGAGCTTA CGAGCCTAAA
    CTAACACCTG AAGAAATGAT GGATTCTTCA ACCTTCAAAA GATTTATTGC TTCAATAGAG
    AATATTCTGG AAAATCTGGA AGACATGGAT TTTACAACCT TGGGTGATGA TGATGAGATT
    CCACAGGAAT TGCTTTTGGG AAAACATCAG CTTAATGAGT TGGGTAGTGA ATCTGCAAAA
    ATCAAGGCAA TGGGTATTAT GGACAAGCTC TCAACAGATA AAATAGTAAA GGTCCTGAAC
    ATTTTGGAGA AGAATATTCA AGACGGATCA AAGCTTTCTA CATTACTTAA CCACAACAAT
    GACACTGAAG ATGAGGAGAG ATTATGGAGA GATCTCATAA TGGAGAGAGT AACAAAATCT
    GCAGATGCTT GTCTTACTGC TATCAATATT ATGACATCAC CAAATATGCC TAAAGCTGTT
    TATATTGAGG ACGTAATAGA AAGAGTTATT CAGTACACAA AATTTCATTT GCAGAACACT
    CTCTATCCTC AGTATGACCC TGTGTATAGA GTGGATCCTC TTGGTGGTGG TCTTTTAAGT
    TCAAAAGCAA AACGTGCCAA GTGTTCCACC CACAAACAAA GAGTTATAGT GATGTTGTAT
    AACAAAGTTT GTGATATTGT CAGCAGTTTG TCAGAGTTGC TAGAGATACA ACTTCTTACT
    GATACAACTA TTCTTCAGGT ATCGTCTATG GGGATAACAC CTTTCTTTGT AGAAAATGTC
    AGTGAACTAC AGCTATGTGC CATCAAATTA GTGACTGCAG TCTTCTCCAG GTATGAAAAA
    CATAGACAGC TAATACTAGA AGAAATTTTT ACTTCCCTTG CAAGACTACC AACTAGCAAG
    AGGAGCCTGA GAAACTTCAG GTTGAATAGC AGTGACACAG ATGGAGAGCC TATGTATATT
    CAGATGGTAA CAGCACTAGT TCTGCAGCTT ATTCAATGTG TCGTGCACTT ACCATCAGCA
    GAAAAAGATT CTAATTCAGA TGAGGAATTG AACAAAAAAG TGGATCAAGA TGTGCTTATT
    ACTAACTCAT ATGAAACAGC TATGAGAACA GCACAAAATT TTCTTTCTAT TTTTCTTAAG
    AAATGTGGTA GTAAACAAGG AGAAGAAGAT TACAGGCCTC TCTTTGAGAA CTTCATTCAA
    GATCTTCTTT CCACAGTCAA TAAACCAGAA TGGCCAGCTG CAGAGTTGCT GCTTAGCTTA
    CTAGGAAGGT TATTGGTTCA CCAGTTCAGT AATAAATCAA CAGAAATGGC TTTAAGAGTT
    GCGTCACTTG ACTATCTTGG AACCGTAGCT GCAAGACTGA GGAAAGATGC AGTCACTAGT
    AAAATGGATC AAGGATCTAT AGCTAGAATC CTCAAACAGG CCTCAGGAGG AGAAGATGAT
    ATTCAACAGC TACAGAAGGC TTTATTGGAT TATCTGGATG AGAACACTGA AACTGATGCA
    TCGTTAGTGT TTTCTCGGAA GTTTTATATA GCTCAGTGGT TTCGGGATAC AACTATGGAG
    ACAGAGAAGG CAATTAAATC TCAGAAGGAT GAGGATTCAT CTGAAGGAAC TCATCATGCA
    AAAGATGTTG AGACCACAGG ACAAATCATG CACAGAGCAG AAAGCCGCAA GAAGTTTCTC
    CGCAGCATCA TTAAAACTGC TCCATCTCAG TTCAGTACAT TAAAGATTTA TTCTGACACT
    GTGGACTATG AAGATGCATG TTTGATTGTT CGCTACTTGG CCTCTATGAG GCCGTTTGCC
    CAGAGCTTCG ATATTTATTT AACACAGATT CTGCGTGTAC TTGGTGAAAA TGCAATTGCA
    GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGATCC CAGTATTCTA
    GCCAGGCCGG ATATGCAGCG AGGTGTTCAT GGACGGTTAA TGGATAACTC CACCAGTGTA
    CGGGAAGCAG CTGTGGAGCT GCTTGGCCGA TTTGTGCTCT GTCGTCCTCA GCTTGCTGAG
    CAGTATTATG ACATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGAAAAAGA
    GTCATAAAGA TACTTAGGGA TATCTGCATT GAACAACCAA CGTTTCCCAA AATTACAGAG
    ATGTGTGTGA AAATGATTCG GAGAGTCAAT GATGAAGAAG GCATTAAGAA ATTAGTAAAT
    GAGACATTCC AGAAGCTGTG GTTTACTCCA ACTCCACACC ATGACAAAGA AGCAATGACT
    AGGAAAATAC TGAACATCAC TGATGTGGTG GCAGCTTGTA GGGATACAGG GTATGATTGG
    TTTGAGCAGC TTCTTCAAAA TCTATTGAAG TCAGAAGAGG ATGCTTCATA TAAACCTGTG
    AAGAAGGCTT GTACTCAACT TGTTGACAAT TTGGTTGAGC ACATCCTTAA ATATGAGGAG
    TCTTTATCAG ACTCTGACAA CAAAGGAGTG AATTCTGGTC GCTTGGTTGC TTGTATAACC
    ACTCTGTTCT TATTCAGCAA AATCAGGCCC CAGTTAATGG TCAAACATGC CATGACCATG
    CAGCCATATC TGGCAACTAA ATGTAGTACT CAGAATGATT TCATGGTGAT CTGCAATGTT
    GCAAAAATCT TGGAGCTTGT AGTGCCGTTA ATGGAACACC CAAGTGAGAC CTTCCTTGCC
    ACTATTGAGG AAGACCTCAT GAAACTCATC ATCAAATATG GCATGACAGT TGTTCAACAT
    TGTGTGAGCT GCCTTGGGTC TGTTGTGAAC AAGGTCACCC AGAATTACAA GTTTGTGTGG
    GCCTGTTTTA ATAGATACTA TGGTGCACTT TCAAAATTAA AAAGTCAACA CCAAGAAGAC
    CCCAACAGTA CAATACTTAC TGCCAATAAA CCAGCACTCC TTAGATCACT TTTCACTGTT
    GGAGCACTGT GTCGGCATTT TGATTTTGAC CATGAAGATT TTAAGGGCAA CAGTAAGGTT
    AACATTAAGG ATAAAGTACT TGAACTGCTG ATGTATTTTA CAAAGCATTC AGACGAAGAA
    GTACAGACAA AAGCCATTAT TGGCCTGGGA TTTGCATTTA TACAACACCC AAGCTTAATG
    TTTGAACAGG AAGTAAAGAC TCTGTACAAC AGCATTCTTT CAGATAAGAA CTGTTCAGTA
    AACCTAAAAA TCCAGGTGTT AAAAAACCTC CAGACCTACT TGCAGGAGGA GGACACACGT
    ATGCAGCAGG CAGACAGAGA CTGGAAAAAA GTAGCAAAAC AGGAAGACCT GAAAGAAATG
    GGTGATATTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TGTATCTCAA ACAGGTCCTT
    GAGGCTTTCT TTCATACCCA GTCCAATGTA CGCCACTTTG CTCTAAATGT CATTGCACTG
    ACGTTAAACC AGGGTCTCAT CCATCCTGTT CAGTGTGTGC CATACTTAAT TGCTATGGGC
    ACAGATCCAG AGCCTTCTAT GCGAAACAAA GCTGACCAGC AGTTGGTGGA AATAGACAAA
    AAGTATGCTG GTTTCATTCA CATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
    CAACAGGCAA TCAATACCTG CCCAAAGGAT CCTGTAAGAG GCTTTAGACA CGATGAATCA
    TCCAGTGCAT TGTGTTCACA CCTATATTCT ATGATCCGAG GGAACCGTCA ACACAGACGA
    GCATTTCTAA TTTCTTTACT CAATCTCTTC GATGATACTG CAAAAACAGA GGTGAATATG
    CTCTTGTACA TAGCAGACAA TCTAGCCTGT TTTCCTTATC AAACGCAGGA AGAGCCACTG
    TTTATAATGC ATCATATAGA CATTACACTA TCAGTTTCTG GTAGCAACCT ACTACAGTCA
    TTTAAAGAGT CAATGGTGAA AGACAAAAAA AAGGAAAGAA AGCCTTCATC AGATGAGGAG
    ACTGAGAGTG ACAGTAATAG TGGCAGTGAG AGCGAAAGTG AAGAGGAAGC TTCTAAGCCT
    CGGAGGTTAC GAAAACGAGT AGATTCTGAC TCAGATTCTG ATGAAGAAAA TGATATAAAT
    GCTGTGATGA AATGTTTACC AGAAAACTCA GCTCCTTTAA TTGAATTTGC AAATGTGTCT
    CAAGGCATAT TATTACTTTT GATGCTGAAA CAGCATTTAA AGAACCTTTG CGGATTCTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCT GAGTCTGCAA AAGTTTATGA TAAAGCGATA
    AACAGGAAGA CAGGAGTGCA TTTCCATCCA AAACAAACTC TGGATTTTCT ACGGAGTGAT
    ATGGCTAATT CCAAGATCAC TGAAGAAGTG AAGAGGAGTA TAGTAAAACA GTACTTAGAT
    TTCAAGCTCC TTATGGAACA TTTGGATCCT GATGAAGAAG AAGAAGATGG AGAGGTGTCA
    GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AACAATGCAG CTGAGACAGA GGACGATGAA AGTGATGGGG AGGATAGAGG AGGAGGCACT
    TCAGGGTCAT TGAGAAGGTC AAAACGAAAT TCAGACTCTA CGGAGTTGGC TTCACAGATG
    AATGAAAGTG TTGATGTCAT GGATGTCATC GCTATTTGCT GTCCAAAGTA CAAAGATCGA
    CCACAAATTG CAAGAGTAGT ACAGAAAACC AGCAATGGCT TCAGTGTTCA GTGGATGGCA
    GGCTCCTACA GTGGCTCCTG GACTGAGGCT AAGCGCCGTG ATGGCCGCAA ACTGGTGCCT
    TGGGTAGACA CTATTAAAGA GTCAGACATT ATTTACAAAA AAATTGCTCT AACGAGTGCT
    AATAAGCTGA CTAATAAAGT TGTTCAGACT TTACGATCGC TGTATGCCGC CAAGGATGGA
    ACTTCGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_425012.3
    CDS520..8871
    Translation

    Target ORF information:

    RefSeq Version XM_425012.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_425012.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGATAAGCCT
    CTGAAGAAGC GAAAACAAGA AAACAAACCA CAGGAGGCTG GGGGTGCTGC TGGAGGAAAT
    AGGCCAGCTT CTCAGGAGTC AGGTTCTGCA GGAAATGGGG CAAGGCCAGC ACTGATGGTT
    AGTATTGATC TTCAACAAGC AGGAAGAGCG GACTCTCAGG CAACAATAAC TCAGGAATTT
    GAGGCTATCA AAAAACCTGA AGAGATTAAG CAGTATAATG ATGGATTTGT GTGTATTCCC
    CAAGAAGAGA CTGTTGGAGT TCTTAAACTT AAACCTGAAA ACCACCCTGA GATTTTTAGG
    AAAAAGTCAG ACTTTGAGGT TCAAAAGACA GATATCCAGC AAAATGAAAA CAGACAAATG
    GAGTTTCAGC AAAATGAGAG CAGAAGGTTG GAAAGTAAAT ACAATGACAA TAAACAGGTA
    GAAGCTAAAT ATGACATCAA GCATGACAGT AGACAAATGG AGATGAAACA AAATGAGAAC
    AGACAGGCAG AGTCAAAACA AAATGAGAGC AGACAAATGG AGATGAAACA AAATGAGGGA
    AAACAAAATA ATGGCAAACA AGAAATAAGA CAGAGACCTG AAACACCAAA GCAGAAGAGC
    GAAGGCAGGC CTGAAACACC CAAGCAGAAG AGTGAAGGCA GGCCTGAAAC ACCAAAACAT
    AGGCATGATA ACAGGAGGGA CTCCAGTAAA CCATTGTCAG AAAAGAAAAT TGAATTATCT
    AGGCATAAAC CTGATGTGAA ATCTGATACT TCAAAGGTAA AATCTGATAG TAGATCAGAT
    CCCGTGAAGC AAAGGCCTGA TGGGCGTTCA GTTTCTGATA CTCAGAGGCG TGACCACGAT
    AGCCTTAAAC AGAGATCTGA GGACAGGGAA TCAGAAAGAT ACAAAGGAGA TCCATCCAAG
    ATTAGAAGGA CTGAATTTTT GAGATCCTCA AACAGAAATG AACATGATTC TAAGTATGGT
    ATTAAATCTG ATGGTTCAAA ACCTGAAAAA TTTGAAAGGA AACACAGACA TGACTCTGGT
    GAATCAAGGG AAAGATTTTC TTCTGGGGAT CAGAAGTCAG ATCAAAAGTC AAGACCTGAC
    AGCCCTCGAG TTAAACAGGA TGGCAAAGGA GATTCTAGCA AATCAAGACC GGATAAACCT
    GGTTTTAGAT CACCCAACAG CAAGGATGAA CGAAGGACAG ACAGTAGTAA GAGTAAAGTA
    GAGAGTAATA AAGCACATCC TGACAATAAA GCAGAGTTTC CCAGTTATCT GCTGGGGGCA
    AGATCTGGTG CACTGAAACA TTTTGTCATT CCAAAAATCA AACGTGACAA AGATGGCAAT
    GTCACTCAGG AGTCAAGGAA AACTGAACTT AAAGGTGAAC AGAAGGACAA AGTAGAGAAG
    ATTGGGCTAG TTGAAGACCT AAATAAAGGA GCTAAGCCTG TAGTTGTCCT TCAGAAGCTT
    TCTTTGGACG ATGTACAAAA ATTTATTAAG GATAGAGAAG ATAAATCAAG GGGCTCTTGT
    TTTAAACCTA ACAAGAACAA GTCGTCCAAA TCTAATAAAG GTAGCATAGA TCAGTCAGTG
    CTAAAGGAGT TGCCTCCTGA GCTCCTGGCA GAAATTGAAT CCACCATGCC TCTTTGTGAA
    CGCGTGAAAA TGAACAAGCG CAAACGTAGC ACAGTTAATG AGAAACCAAA ATATGCTGAA
    ATCAGTTCTG ATGAAGACAA TGACAGTGAA GAAGCTTTTG AATCTTCTCG AAAAAGACAT
    AAAAAGGATA GAGATGAAGC TTGGGAGTAC GAAGAGCATG ACAGAAGAAG TTCTGGTGAC
    CACAGGAGGA GTGGTCATTA TCATGAAGGA AGAAGGACTT CTGGTAGTGG CCGTTACCGC
    AATCGCAGTC CCGATGACTC TGATCTGGAT GATTATTCTC CTCCTCCTAG CCTCAGTGAG
    GTGGCAAGAA AAATGAAGAA AAAAGAAAAG CAAAAGAAGA GGAGAGCTTA CGAGCCTAAA
    CTAACACCTG AAGAAATGAT GGATTCTTCA ACCTTCAAAA GATTTATTGC TTCAATAGAG
    AATATTCTGG AAAATCTGGA AGACATGGAT TTTACAACCT TGGGTGATGA TGATGAGATT
    CCACAGGAAT TGCTTTTGGG AAAACATCAG CTTAATGAGT TGGGTAGTGA ATCTGCAAAA
    ATCAAGGCAA TGGGTATTAT GGACAAGCTC TCAACAGATA AAATAGTAAA GGTCCTGAAC
    ATTTTGGAGA AGAATATTCA AGACGGATCA AAGCTTTCTA CATTACTTAA CCACAACAAT
    GACACTGAAG ATGAGGAGAG ATTATGGAGA GATCTCATAA TGGAGAGAGT AACAAAATCT
    GCAGATGCTT GTCTTACTGC TATCAATATT ATGACATCAC CAAATATGCC TAAAGCTGTT
    TATATTGAGG ACGTAATAGA AAGAGTTATT CAGTACACAA AATTTCATTT GCAGAACACT
    CTCTATCCTC AGTATGACCC TGTGTATAGA GTGGATCCTC TTGGTGGTGG TCTTTTAAGT
    TCAAAAGCAA AACGTGCCAA GTGTTCCACC CACAAACAAA GAGTTATAGT GATGTTGTAT
    AACAAAGTTT GTGATATTGT CAGCAGTTTG TCAGAGTTGC TAGAGATACA ACTTCTTACT
    GATACAACTA TTCTTCAGGT ATCGTCTATG GGGATAACAC CTTTCTTTGT AGAAAATGTC
    AGTGAACTAC AGCTATGTGC CATCAAATTA GTGACTGCAG TCTTCTCCAG GTATGAAAAA
    CATAGACAGC TAATACTAGA AGAAATTTTT ACTTCCCTTG CAAGACTACC AACTAGCAAG
    AGGAGCCTGA GAAACTTCAG GTTGAATAGC AGTGACACAG ATGGAGAGCC TATGTATATT
    CAGATGGTAA CAGCACTAGT TCTGCAGCTT ATTCAATGTG TCGTGCACTT ACCATCAGCA
    GAAAAAGATT CTAATTCAGA TGAGGAATTG AACAAAAAAG TGGATCAAGA TGTGCTTATT
    ACTAACTCAT ATGAAACAGC TATGAGAACA GCACAAAATT TTCTTTCTAT TTTTCTTAAG
    AAATGTGGTA GTAAACAAGG AGAAGAAGAT TACAGGCCTC TCTTTGAGAA CTTCATTCAA
    GATCTTCTTT CCACAGTCAA TAAACCAGAA TGGCCAGCTG CAGAGTTGCT GCTTAGCTTA
    CTAGGAAGGT TATTGGTTCA CCAGTTCAGT AATAAATCAA CAGAAATGGC TTTAAGAGTT
    GCGTCACTTG ACTATCTTGG AACCGTAGCT GCAAGACTGA GGAAAGATGC AGTCACTAGT
    AAAATGGATC AAGGATCTAT AGCTAGAATC CTCAAACAGG CCTCAGGAGG AGAAGATGAT
    ATTCAACAGC TACAGAAGGC TTTATTGGAT TATCTGGATG AGAACACTGA AACTGATGCA
    TCGTTAGTGT TTTCTCGGAA GTTTTATATA GCTCAGTGGT TTCGGGATAC AACTATGGAG
    ACAGAGAAGG CAATTAAATC TCAGAAGGAT GAGGATTCAT CTGAAGGAAC TCATCATGCA
    AAAGATGTTG AGACCACAGG ACAAATCATG CACAGAGCAG AAAGCCGCAA GAAGTTTCTC
    CGCAGCATCA TTAAAACTGC TCCATCTCAG TTCAGTACAT TAAAGATTTA TTCTGACACT
    GTGGACTATG AAGATGCATG TTTGATTGTT CGCTACTTGG CCTCTATGAG GCCGTTTGCC
    CAGAGCTTCG ATATTTATTT AACACAGATT CTGCGTGTAC TTGGTGAAAA TGCAATTGCA
    GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGATCC CAGTATTCTA
    GCCAGGCCGG ATATGCAGCG AGGTGTTCAT GGACGGTTAA TGGATAACTC CACCAGTGTA
    CGGGAAGCAG CTGTGGAGCT GCTTGGCCGA TTTGTGCTCT GTCGTCCTCA GCTTGCTGAG
    CAGTATTATG ACATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGAAAAAGA
    GTCATAAAGA TACTTAGGGA TATCTGCATT GAACAACCAA CGTTTCCCAA AATTACAGAG
    ATGTGTGTGA AAATGATTCG GAGAGTCAAT GATGAAGAAG GCATTAAGAA ATTAGTAAAT
    GAGACATTCC AGAAGCTGTG GTTTACTCCA ACTCCACACC ATGACAAAGA AGCAATGACT
    AGGAAAATAC TGAACATCAC TGATGTGGTG GCAGCTTGTA GGGATACAGG GTATGATTGG
    TTTGAGCAGC TTCTTCAAAA TCTATTGAAG TCAGAAGAGG ATGCTTCATA TAAACCTGTG
    AAGAAGGCTT GTACTCAACT TGTTGACAAT TTGGTTGAGC ACATCCTTAA ATATGAGGAG
    TCTTTATCAG ACTCTGACAA CAAAGGAGTG AATTCTGGTC GCTTGGTTGC TTGTATAACC
    ACTCTGTTCT TATTCAGCAA AATCAGGCCC CAGTTAATGG TCAAACATGC CATGACCATG
    CAGCCATATC TGGCAACTAA ATGTAGTACT CAGAATGATT TCATGGTGAT CTGCAATGTT
    GCAAAAATCT TGGAGCTTGT AGTGCCGTTA ATGGAACACC CAAGTGAGAC CTTCCTTGCC
    ACTATTGAGG AAGACCTCAT GAAACTCATC ATCAAATATG GCATGACAGT TGTTCAACAT
    TGTGTGAGCT GCCTTGGGTC TGTTGTGAAC AAGGTCACCC AGAATTACAA GTTTGTGTGG
    GCCTGTTTTA ATAGATACTA TGGTGCACTT TCAAAATTAA AAAGTCAACA CCAAGAAGAC
    CCCAACAGTA CAATACTTAC TGCCAATAAA CCAGCACTCC TTAGATCACT TTTCACTGTT
    GGAGCACTGT GTCGGCATTT TGATTTTGAC CATGAAGATT TTAAGGGCAA CAGTAAGGTT
    AACATTAAGG ATAAAGTACT TGAACTGCTG ATGTATTTTA CAAAGCATTC AGACGAAGAA
    GTACAGACAA AAGCCATTAT TGGCCTGGGA TTTGCATTTA TACAACACCC AAGCTTAATG
    TTTGAACAGG AAGTAAAGAC TCTGTACAAC AGCATTCTTT CAGATAAGAA CTGTTCAGTA
    AACCTAAAAA TCCAGGTGTT AAAAAACCTC CAGACCTACT TGCAGGAGGA GGACACACGT
    ATGCAGCAGG CAGACAGAGA CTGGAAAAAA GTAGCAAAAC AGGAAGACCT GAAAGAAATG
    GGTGATATTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TGTATCTCAA ACAGGTCCTT
    GAGGCTTTCT TTCATACCCA GTCCAATGTA CGCCACTTTG CTCTAAATGT CATTGCACTG
    ACGTTAAACC AGGGTCTCAT CCATCCTGTT CAGTGTGTGC CATACTTAAT TGCTATGGGC
    ACAGATCCAG AGCCTTCTAT GCGAAACAAA GCTGACCAGC AGTTGGTGGA AATAGACAAA
    AAGTATGCTG GTTTCATTCA CATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
    CAACAGGCAA TCAATACCTG CCCAAAGGAT CCTGTAAGAG GCTTTAGACA CGATGAATCA
    TCCAGTGCAT TGTGTTCACA CCTATATTCT ATGATCCGAG GGAACCGTCA ACACAGACGA
    GCATTTCTAA TTTCTTTACT CAATCTCTTC GATGATACTG CAAAAACAGA GGTGAATATG
    CTCTTGTACA TAGCAGACAA TCTAGCCTGT TTTCCTTATC AAACGCAGGA AGAGCCACTG
    TTTATAATGC ATCATATAGA CATTACACTA TCAGTTTCTG GTAGCAACCT ACTACAGTCA
    TTTAAAGAGT CAATGGTGAA AGACAAAAAA AAGGAAAGAA AGCCTTCATC AGATGAGGAG
    ACTGAGAGTG ACAGTAATAG TGGCAGTGAG AGCGAAAGTG AAGAGGAAGC TTCTAAGCCT
    CGGAGGTTAC GAAAACGAGT AGATTCTGAC TCAGATTCTG ATGAAGAAAA TGATATAAAT
    GCTGTGATGA AATGTTTACC AGAAAACTCA GCTCCTTTAA TTGAATTTGC AAATGTGTCT
    CAAGGCATAT TATTACTTTT GATGCTGAAA CAGCATTTAA AGAACCTTTG CGGATTCTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCT GAGTCTGCAA AAGTTTATGA TAAAGCGATA
    AACAGGAAGA CAGGAGTGCA TTTCCATCCA AAACAAACTC TGGATTTTCT ACGGAGTGAT
    ATGGCTAATT CCAAGATCAC TGAAGAAGTG AAGAGGAGTA TAGTAAAACA GTACTTAGAT
    TTCAAGCTCC TTATGGAACA TTTGGATCCT GATGAAGAAG AAGAAGATGG AGAGGTGTCA
    GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AACAATGCAG CTGAGACAGA GGACGATGAA AGTGATGGGG AGGATAGAGG AGGAGGCACT
    TCAGGGTCAT TGAGAAGGTC AAAACGAAAT TCAGACTCTA CGGAGTTGGC TTCACAGATG
    AATGAAAGTG TTGATGTCAT GGATGTCATC GCTATTTGCT GTCCAAAGTA CAAAGATCGA
    CCACAAATTG CAAGAGTAGT ACAGAAAACC AGCAATGGCT TCAGTGTTCA GTGGATGGCA
    GGCTCCTACA GTGGCTCCTG GACTGAGGCT AAGCGCCGTG ATGGCCGCAA ACTGGTGCCT
    TGGGTAGACA CTATTAAAGA GTCAGACATT ATTTACAAAA AAATTGCTCT AACGAGTGCT
    AATAAGCTGA CTAATAAAGT TGTTCAGACT TTACGATCGC TGTATGCCGC CAAGGATGGA
    ACTTCGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa20946
    Clone ID Related Accession (Same CDS sequence) XM_425012.5 , XM_425012.6 , XM_015277519.2 , XM_015277519.1 , XM_015277520.1 , XM_015277520.2
    Accession Version XM_015277519.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8352bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product nipped-B-like protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGATAAGCCT
    CTGAAGAAGC GAAAACAAGA AAACAAACCA CAGGAGGCTG GGGGTGCTGC TGGAGGAAAT
    AGGCCAGCTT CTCAGGAGTC AGGTTCTGCA GGAAATGGGG CAAGGCCAGC ACTGATGGTT
    AGTATTGATC TTCAACAAGC AGGAAGAGCG GACTCTCAGG CAACAATAAC TCAGGAATTT
    GAGGCTATCA AAAAACCTGA AGAGATTAAG CAGTATAATG ATGGATTTGT GTGTATTCCC
    CAAGAAGAGA CTGTTGGAGT TCTTAAACTT AAACCTGAAA ACCACCCTGA GATTTTTAGG
    AAAAAGTCAG ACTTTGAGGT TCAAAAGACA GATATCCAGC AAAATGAAAA CAGACAAATG
    GAGTTTCAGC AAAATGAGAG CAGAAGGTTG GAAAGTAAAT ACAATGACAA TAAACAGGTA
    GAAGCTAAAT ATGACATCAA GCATGACAGT AGACAAATGG AGATGAAACA AAATGAGAAC
    AGACAGGCAG AGTCAAAACA AAATGAGAGC AGACAAATGG AGATGAAACA AAATGAGGGA
    AAACAAAATA ATGGCAAACA AGAAATAAGA CAGAGACCTG AAACACCAAA GCAGAAGAGC
    GAAGGCAGGC CTGAAACACC CAAGCAGAAG AGTGAAGGCA GGCCTGAAAC ACCAAAACAT
    AGGCATGATA ACAGGAGGGA CTCCAGTAAA CCATTGTCAG AAAAGAAAAT TGAATTATCT
    AGGCATAAAC CTGATGTGAA ATCTGATACT TCAAAGGTAA AATCTGATAG TAGATCAGAT
    CCCGTGAAGC AAAGGCCTGA TGGGCGTTCA GTTTCTGATA CTCAGAGGCG TGACCACGAT
    AGCCTTAAAC AGAGATCTGA GGACAGGGAA TCAGAAAGAT ACAAAGGAGA TCCATCCAAG
    ATTAGAAGGA CTGAATTTTT GAGATCCTCA AACAGAAATG AACATGATTC TAAGTATGGT
    ATTAAATCTG ATGGTTCAAA ACCTGAAAAA TTTGAAAGGA AACACAGACA TGACTCTGGT
    GAATCAAGGG AAAGATTTTC TTCTGGGGAT CAGAAGTCAG ATCAAAAGTC AAGACCTGAC
    AGCCCTCGAG TTAAACAGGA TGGCAAAGGA GATTCTAGCA AATCAAGACC GGATAAACCT
    GGTTTTAGAT CACCCAACAG CAAGGATGAA CGAAGGACAG ACAGTAGTAA GAGTAAAGTA
    GAGAGTAATA AAGCACATCC TGACAATAAA GCAGAGTTTC CCAGTTATCT GCTGGGGGCA
    AGATCTGGTG CACTGAAACA TTTTGTCATT CCAAAAATCA AACGTGACAA AGATGGCAAT
    GTCACTCAGG AGTCAAGGAA AACTGAACTT AAAGGTGAAC AGAAGGACAA AGTAGAGAAG
    ATTGGGCTAG TTGAAGACCT AAATAAAGGA GCTAAGCCTG TAGTTGTCCT TCAGAAGCTT
    TCTTTGGACG ATGTACAAAA ATTTATTAAG GATAGAGAAG ATAAATCAAG GGGCTCTTGT
    TTTAAACCTA ACAAGAACAA GTCGTCCAAA TCTAATAAAG GTAGCATAGA TCAGTCAGTG
    CTAAAGGAGT TGCCTCCTGA GCTCCTGGCA GAAATTGAAT CCACCATGCC TCTTTGTGAA
    CGCGTGAAAA TGAACAAGCG CAAACGTAGC ACAGTTAATG AGAAACCAAA ATATGCTGAA
    ATCAGTTCTG ATGAAGACAA TGACAGTGAA GAAGCTTTTG AATCTTCTCG AAAAAGACAT
    AAAAAGGATA GAGATGAAGC TTGGGAGTAC GAAGAGCATG ACAGAAGAAG TTCTGGTGAC
    CACAGGAGGA GTGGTCATTA TCATGAAGGA AGAAGGACTT CTGGTAGTGG CCGTTACCGC
    AATCGCAGTC CCGATGACTC TGATCTGGAT GATTATTCTC CTCCTCCTAG CCTCAGTGAG
    GTGGCAAGAA AAATGAAGAA AAAAGAAAAG CAAAAGAAGA GGAGAGCTTA CGAGCCTAAA
    CTAACACCTG AAGAAATGAT GGATTCTTCA ACCTTCAAAA GATTTATTGC TTCAATAGAG
    AATATTCTGG AAAATCTGGA AGACATGGAT TTTACAACCT TGGGTGATGA TGATGAGATT
    CCACAGGAAT TGCTTTTGGG AAAACATCAG CTTAATGAGT TGGGTAGTGA ATCTGCAAAA
    ATCAAGGCAA TGGGTATTAT GGACAAGCTC TCAACAGATA AAATAGTAAA GGTCCTGAAC
    ATTTTGGAGA AGAATATTCA AGACGGATCA AAGCTTTCTA CATTACTTAA CCACAACAAT
    GACACTGAAG ATGAGGAGAG ATTATGGAGA GATCTCATAA TGGAGAGAGT AACAAAATCT
    GCAGATGCTT GTCTTACTGC TATCAATATT ATGACATCAC CAAATATGCC TAAAGCTGTT
    TATATTGAGG ACGTAATAGA AAGAGTTATT CAGTACACAA AATTTCATTT GCAGAACACT
    CTCTATCCTC AGTATGACCC TGTGTATAGA GTGGATCCTC TTGGTGGTGG TCTTTTAAGT
    TCAAAAGCAA AACGTGCCAA GTGTTCCACC CACAAACAAA GAGTTATAGT GATGTTGTAT
    AACAAAGTTT GTGATATTGT CAGCAGTTTG TCAGAGTTGC TAGAGATACA ACTTCTTACT
    GATACAACTA TTCTTCAGGT ATCGTCTATG GGGATAACAC CTTTCTTTGT AGAAAATGTC
    AGTGAACTAC AGCTATGTGC CATCAAATTA GTGACTGCAG TCTTCTCCAG GTATGAAAAA
    CATAGACAGC TAATACTAGA AGAAATTTTT ACTTCCCTTG CAAGACTACC AACTAGCAAG
    AGGAGCCTGA GAAACTTCAG GTTGAATAGC AGTGACACAG ATGGAGAGCC TATGTATATT
    CAGATGGTAA CAGCACTAGT TCTGCAGCTT ATTCAATGTG TCGTGCACTT ACCATCAGCA
    GAAAAAGATT CTAATTCAGA TGAGGAATTG AACAAAAAAG TGGATCAAGA TGTGCTTATT
    ACTAACTCAT ATGAAACAGC TATGAGAACA GCACAAAATT TTCTTTCTAT TTTTCTTAAG
    AAATGTGGTA GTAAACAAGG AGAAGAAGAT TACAGGCCTC TCTTTGAGAA CTTCATTCAA
    GATCTTCTTT CCACAGTCAA TAAACCAGAA TGGCCAGCTG CAGAGTTGCT GCTTAGCTTA
    CTAGGAAGGT TATTGGTTCA CCAGTTCAGT AATAAATCAA CAGAAATGGC TTTAAGAGTT
    GCGTCACTTG ACTATCTTGG AACCGTAGCT GCAAGACTGA GGAAAGATGC AGTCACTAGT
    AAAATGGATC AAGGATCTAT AGCTAGAATC CTCAAACAGG CCTCAGGAGG AGAAGATGAT
    ATTCAACAGC TACAGAAGGC TTTATTGGAT TATCTGGATG AGAACACTGA AACTGATGCA
    TCGTTAGTGT TTTCTCGGAA GTTTTATATA GCTCAGTGGT TTCGGGATAC AACTATGGAG
    ACAGAGAAGG CAATTAAATC TCAGAAGGAT GAGGATTCAT CTGAAGGAAC TCATCATGCA
    AAAGATGTTG AGACCACAGG ACAAATCATG CACAGAGCAG AAAGCCGCAA GAAGTTTCTC
    CGCAGCATCA TTAAAACTGC TCCATCTCAG TTCAGTACAT TAAAGATTTA TTCTGACACT
    GTGGACTATG AAGATGCATG TTTGATTGTT CGCTACTTGG CCTCTATGAG GCCGTTTGCC
    CAGAGCTTCG ATATTTATTT AACACAGATT CTGCGTGTAC TTGGTGAAAA TGCAATTGCA
    GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGATCC CAGTATTCTA
    GCCAGGCCGG ATATGCAGCG AGGTGTTCAT GGACGGTTAA TGGATAACTC CACCAGTGTA
    CGGGAAGCAG CTGTGGAGCT GCTTGGCCGA TTTGTGCTCT GTCGTCCTCA GCTTGCTGAG
    CAGTATTATG ACATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGAAAAAGA
    GTCATAAAGA TACTTAGGGA TATCTGCATT GAACAACCAA CGTTTCCCAA AATTACAGAG
    ATGTGTGTGA AAATGATTCG GAGAGTCAAT GATGAAGAAG GCATTAAGAA ATTAGTAAAT
    GAGACATTCC AGAAGCTGTG GTTTACTCCA ACTCCACACC ATGACAAAGA AGCAATGACT
    AGGAAAATAC TGAACATCAC TGATGTGGTG GCAGCTTGTA GGGATACAGG GTATGATTGG
    TTTGAGCAGC TTCTTCAAAA TCTATTGAAG TCAGAAGAGG ATGCTTCATA TAAACCTGTG
    AAGAAGGCTT GTACTCAACT TGTTGACAAT TTGGTTGAGC ACATCCTTAA ATATGAGGAG
    TCTTTATCAG ACTCTGACAA CAAAGGAGTG AATTCTGGTC GCTTGGTTGC TTGTATAACC
    ACTCTGTTCT TATTCAGCAA AATCAGGCCC CAGTTAATGG TCAAACATGC CATGACCATG
    CAGCCATATC TGGCAACTAA ATGTAGTACT CAGAATGATT TCATGGTGAT CTGCAATGTT
    GCAAAAATCT TGGAGCTTGT AGTGCCGTTA ATGGAACACC CAAGTGAGAC CTTCCTTGCC
    ACTATTGAGG AAGACCTCAT GAAACTCATC ATCAAATATG GCATGACAGT TGTTCAACAT
    TGTGTGAGCT GCCTTGGGTC TGTTGTGAAC AAGGTCACCC AGAATTACAA GTTTGTGTGG
    GCCTGTTTTA ATAGATACTA TGGTGCACTT TCAAAATTAA AAAGTCAACA CCAAGAAGAC
    CCCAACAGTA CAATACTTAC TGCCAATAAA CCAGCACTCC TTAGATCACT TTTCACTGTT
    GGAGCACTGT GTCGGCATTT TGATTTTGAC CATGAAGATT TTAAGGGCAA CAGTAAGGTT
    AACATTAAGG ATAAAGTACT TGAACTGCTG ATGTATTTTA CAAAGCATTC AGACGAAGAA
    GTACAGACAA AAGCCATTAT TGGCCTGGGA TTTGCATTTA TACAACACCC AAGCTTAATG
    TTTGAACAGG AAGTAAAGAC TCTGTACAAC AGCATTCTTT CAGATAAGAA CTGTTCAGTA
    AACCTAAAAA TCCAGGTGTT AAAAAACCTC CAGACCTACT TGCAGGAGGA GGACACACGT
    ATGCAGCAGG CAGACAGAGA CTGGAAAAAA GTAGCAAAAC AGGAAGACCT GAAAGAAATG
    GGTGATATTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TGTATCTCAA ACAGGTCCTT
    GAGGCTTTCT TTCATACCCA GTCCAATGTA CGCCACTTTG CTCTAAATGT CATTGCACTG
    ACGTTAAACC AGGGTCTCAT CCATCCTGTT CAGTGTGTGC CATACTTAAT TGCTATGGGC
    ACAGATCCAG AGCCTTCTAT GCGAAACAAA GCTGACCAGC AGTTGGTGGA AATAGACAAA
    AAGTATGCTG GTTTCATTCA CATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
    CAACAGGCAA TCAATACCTG CCCAAAGGAT CCTGTAAGAG GCTTTAGACA CGATGAATCA
    TCCAGTGCAT TGTGTTCACA CCTATATTCT ATGATCCGAG GGAACCGTCA ACACAGACGA
    GCATTTCTAA TTTCTTTACT CAATCTCTTC GATGATACTG CAAAAACAGA GGTGAATATG
    CTCTTGTACA TAGCAGACAA TCTAGCCTGT TTTCCTTATC AAACGCAGGA AGAGCCACTG
    TTTATAATGC ATCATATAGA CATTACACTA TCAGTTTCTG GTAGCAACCT ACTACAGTCA
    TTTAAAGAGT CAATGGTGAA AGACAAAAAA AAGGAAAGAA AGCCTTCATC AGATGAGGAG
    ACTGAGAGTG ACAGTAATAG TGGCAGTGAG AGCGAAAGTG AAGAGGAAGC TTCTAAGCCT
    CGGAGGTTAC GAAAACGAGT AGATTCTGAC TCAGATTCTG ATGAAGAAAA TGATATAAAT
    GCTGTGATGA AATGTTTACC AGAAAACTCA GCTCCTTTAA TTGAATTTGC AAATGTGTCT
    CAAGGCATAT TATTACTTTT GATGCTGAAA CAGCATTTAA AGAACCTTTG CGGATTCTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCT GAGTCTGCAA AAGTTTATGA TAAAGCGATA
    AACAGGAAGA CAGGAGTGCA TTTCCATCCA AAACAAACTC TGGATTTTCT ACGGAGTGAT
    ATGGCTAATT CCAAGATCAC TGAAGAAGTG AAGAGGAGTA TAGTAAAACA GTACTTAGAT
    TTCAAGCTCC TTATGGAACA TTTGGATCCT GATGAAGAAG AAGAAGATGG AGAGGTGTCA
    GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AACAATGCAG CTGAGACAGA GGACGATGAA AGTGATGGGG AGGATAGAGG AGGAGGCACT
    TCAGGGTCAT TGAGAAGGTC AAAACGAAAT TCAGACTCTA CGGAGTTGGC TTCACAGATG
    AATGAAAGTG TTGATGTCAT GGATGTCATC GCTATTTGCT GTCCAAAGTA CAAAGATCGA
    CCACAAATTG CAAGAGTAGT ACAGAAAACC AGCAATGGCT TCAGTGTTCA GTGGATGGCA
    GGCTCCTACA GTGGCTCCTG GACTGAGGCT AAGCGCCGTG ATGGCCGCAA ACTGGTGCCT
    TGGGTAGACA CTATTAAAGA GTCAGACATT ATTTACAAAA AAATTGCTCT AACGAGTGCT
    AATAAGCTGA CTAATAAAGT TGTTCAGACT TTACGATCGC TGTATGCCGC CAAGGATGGA
    ACTTCGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133005.1
    CDS3241..11592
    Translation

    Target ORF information:

    RefSeq Version XM_015277519.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277519.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGATAAGCCT
    CTGAAGAAGC GAAAACAAGA AAACAAACCA CAGGAGGCTG GGGGTGCTGC TGGAGGAAAT
    AGGCCAGCTT CTCAGGAGTC AGGTTCTGCA GGAAATGGGG CAAGGCCAGC ACTGATGGTT
    AGTATTGATC TTCAACAAGC AGGAAGAGCG GACTCTCAGG CAACAATAAC TCAGGAATTT
    GAGGCTATCA AAAAACCTGA AGAGATTAAG CAGTATAATG ATGGATTTGT GTGTATTCCC
    CAAGAAGAGA CTGTTGGAGT TCTTAAACTT AAACCTGAAA ACCACCCTGA GATTTTTAGG
    AAAAAGTCAG ACTTTGAGGT TCAAAAGACA GATATCCAGC AAAATGAAAA CAGACAAATG
    GAGTTTCAGC AAAATGAGAG CAGAAGGTTG GAAAGTAAAT ACAATGACAA TAAACAGGTA
    GAAGCTAAAT ATGACATCAA GCATGACAGT AGACAAATGG AGATGAAACA AAATGAGAAC
    AGACAGGCAG AGTCAAAACA AAATGAGAGC AGACAAATGG AGATGAAACA AAATGAGGGA
    AAACAAAATA ATGGCAAACA AGAAATAAGA CAGAGACCTG AAACACCAAA GCAGAAGAGC
    GAAGGCAGGC CTGAAACACC CAAGCAGAAG AGTGAAGGCA GGCCTGAAAC ACCAAAACAT
    AGGCATGATA ACAGGAGGGA CTCCAGTAAA CCATTGTCAG AAAAGAAAAT TGAATTATCT
    AGGCATAAAC CTGATGTGAA ATCTGATACT TCAAAGGTAA AATCTGATAG TAGATCAGAT
    CCCGTGAAGC AAAGGCCTGA TGGGCGTTCA GTTTCTGATA CTCAGAGGCG TGACCACGAT
    AGCCTTAAAC AGAGATCTGA GGACAGGGAA TCAGAAAGAT ACAAAGGAGA TCCATCCAAG
    ATTAGAAGGA CTGAATTTTT GAGATCCTCA AACAGAAATG AACATGATTC TAAGTATGGT
    ATTAAATCTG ATGGTTCAAA ACCTGAAAAA TTTGAAAGGA AACACAGACA TGACTCTGGT
    GAATCAAGGG AAAGATTTTC TTCTGGGGAT CAGAAGTCAG ATCAAAAGTC AAGACCTGAC
    AGCCCTCGAG TTAAACAGGA TGGCAAAGGA GATTCTAGCA AATCAAGACC GGATAAACCT
    GGTTTTAGAT CACCCAACAG CAAGGATGAA CGAAGGACAG ACAGTAGTAA GAGTAAAGTA
    GAGAGTAATA AAGCACATCC TGACAATAAA GCAGAGTTTC CCAGTTATCT GCTGGGGGCA
    AGATCTGGTG CACTGAAACA TTTTGTCATT CCAAAAATCA AACGTGACAA AGATGGCAAT
    GTCACTCAGG AGTCAAGGAA AACTGAACTT AAAGGTGAAC AGAAGGACAA AGTAGAGAAG
    ATTGGGCTAG TTGAAGACCT AAATAAAGGA GCTAAGCCTG TAGTTGTCCT TCAGAAGCTT
    TCTTTGGACG ATGTACAAAA ATTTATTAAG GATAGAGAAG ATAAATCAAG GGGCTCTTGT
    TTTAAACCTA ACAAGAACAA GTCGTCCAAA TCTAATAAAG GTAGCATAGA TCAGTCAGTG
    CTAAAGGAGT TGCCTCCTGA GCTCCTGGCA GAAATTGAAT CCACCATGCC TCTTTGTGAA
    CGCGTGAAAA TGAACAAGCG CAAACGTAGC ACAGTTAATG AGAAACCAAA ATATGCTGAA
    ATCAGTTCTG ATGAAGACAA TGACAGTGAA GAAGCTTTTG AATCTTCTCG AAAAAGACAT
    AAAAAGGATA GAGATGAAGC TTGGGAGTAC GAAGAGCATG ACAGAAGAAG TTCTGGTGAC
    CACAGGAGGA GTGGTCATTA TCATGAAGGA AGAAGGACTT CTGGTAGTGG CCGTTACCGC
    AATCGCAGTC CCGATGACTC TGATCTGGAT GATTATTCTC CTCCTCCTAG CCTCAGTGAG
    GTGGCAAGAA AAATGAAGAA AAAAGAAAAG CAAAAGAAGA GGAGAGCTTA CGAGCCTAAA
    CTAACACCTG AAGAAATGAT GGATTCTTCA ACCTTCAAAA GATTTATTGC TTCAATAGAG
    AATATTCTGG AAAATCTGGA AGACATGGAT TTTACAACCT TGGGTGATGA TGATGAGATT
    CCACAGGAAT TGCTTTTGGG AAAACATCAG CTTAATGAGT TGGGTAGTGA ATCTGCAAAA
    ATCAAGGCAA TGGGTATTAT GGACAAGCTC TCAACAGATA AAATAGTAAA GGTCCTGAAC
    ATTTTGGAGA AGAATATTCA AGACGGATCA AAGCTTTCTA CATTACTTAA CCACAACAAT
    GACACTGAAG ATGAGGAGAG ATTATGGAGA GATCTCATAA TGGAGAGAGT AACAAAATCT
    GCAGATGCTT GTCTTACTGC TATCAATATT ATGACATCAC CAAATATGCC TAAAGCTGTT
    TATATTGAGG ACGTAATAGA AAGAGTTATT CAGTACACAA AATTTCATTT GCAGAACACT
    CTCTATCCTC AGTATGACCC TGTGTATAGA GTGGATCCTC TTGGTGGTGG TCTTTTAAGT
    TCAAAAGCAA AACGTGCCAA GTGTTCCACC CACAAACAAA GAGTTATAGT GATGTTGTAT
    AACAAAGTTT GTGATATTGT CAGCAGTTTG TCAGAGTTGC TAGAGATACA ACTTCTTACT
    GATACAACTA TTCTTCAGGT ATCGTCTATG GGGATAACAC CTTTCTTTGT AGAAAATGTC
    AGTGAACTAC AGCTATGTGC CATCAAATTA GTGACTGCAG TCTTCTCCAG GTATGAAAAA
    CATAGACAGC TAATACTAGA AGAAATTTTT ACTTCCCTTG CAAGACTACC AACTAGCAAG
    AGGAGCCTGA GAAACTTCAG GTTGAATAGC AGTGACACAG ATGGAGAGCC TATGTATATT
    CAGATGGTAA CAGCACTAGT TCTGCAGCTT ATTCAATGTG TCGTGCACTT ACCATCAGCA
    GAAAAAGATT CTAATTCAGA TGAGGAATTG AACAAAAAAG TGGATCAAGA TGTGCTTATT
    ACTAACTCAT ATGAAACAGC TATGAGAACA GCACAAAATT TTCTTTCTAT TTTTCTTAAG
    AAATGTGGTA GTAAACAAGG AGAAGAAGAT TACAGGCCTC TCTTTGAGAA CTTCATTCAA
    GATCTTCTTT CCACAGTCAA TAAACCAGAA TGGCCAGCTG CAGAGTTGCT GCTTAGCTTA
    CTAGGAAGGT TATTGGTTCA CCAGTTCAGT AATAAATCAA CAGAAATGGC TTTAAGAGTT
    GCGTCACTTG ACTATCTTGG AACCGTAGCT GCAAGACTGA GGAAAGATGC AGTCACTAGT
    AAAATGGATC AAGGATCTAT AGCTAGAATC CTCAAACAGG CCTCAGGAGG AGAAGATGAT
    ATTCAACAGC TACAGAAGGC TTTATTGGAT TATCTGGATG AGAACACTGA AACTGATGCA
    TCGTTAGTGT TTTCTCGGAA GTTTTATATA GCTCAGTGGT TTCGGGATAC AACTATGGAG
    ACAGAGAAGG CAATTAAATC TCAGAAGGAT GAGGATTCAT CTGAAGGAAC TCATCATGCA
    AAAGATGTTG AGACCACAGG ACAAATCATG CACAGAGCAG AAAGCCGCAA GAAGTTTCTC
    CGCAGCATCA TTAAAACTGC TCCATCTCAG TTCAGTACAT TAAAGATTTA TTCTGACACT
    GTGGACTATG AAGATGCATG TTTGATTGTT CGCTACTTGG CCTCTATGAG GCCGTTTGCC
    CAGAGCTTCG ATATTTATTT AACACAGATT CTGCGTGTAC TTGGTGAAAA TGCAATTGCA
    GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGATCC CAGTATTCTA
    GCCAGGCCGG ATATGCAGCG AGGTGTTCAT GGACGGTTAA TGGATAACTC CACCAGTGTA
    CGGGAAGCAG CTGTGGAGCT GCTTGGCCGA TTTGTGCTCT GTCGTCCTCA GCTTGCTGAG
    CAGTATTATG ACATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGAAAAAGA
    GTCATAAAGA TACTTAGGGA TATCTGCATT GAACAACCAA CGTTTCCCAA AATTACAGAG
    ATGTGTGTGA AAATGATTCG GAGAGTCAAT GATGAAGAAG GCATTAAGAA ATTAGTAAAT
    GAGACATTCC AGAAGCTGTG GTTTACTCCA ACTCCACACC ATGACAAAGA AGCAATGACT
    AGGAAAATAC TGAACATCAC TGATGTGGTG GCAGCTTGTA GGGATACAGG GTATGATTGG
    TTTGAGCAGC TTCTTCAAAA TCTATTGAAG TCAGAAGAGG ATGCTTCATA TAAACCTGTG
    AAGAAGGCTT GTACTCAACT TGTTGACAAT TTGGTTGAGC ACATCCTTAA ATATGAGGAG
    TCTTTATCAG ACTCTGACAA CAAAGGAGTG AATTCTGGTC GCTTGGTTGC TTGTATAACC
    ACTCTGTTCT TATTCAGCAA AATCAGGCCC CAGTTAATGG TCAAACATGC CATGACCATG
    CAGCCATATC TGGCAACTAA ATGTAGTACT CAGAATGATT TCATGGTGAT CTGCAATGTT
    GCAAAAATCT TGGAGCTTGT AGTGCCGTTA ATGGAACACC CAAGTGAGAC CTTCCTTGCC
    ACTATTGAGG AAGACCTCAT GAAACTCATC ATCAAATATG GCATGACAGT TGTTCAACAT
    TGTGTGAGCT GCCTTGGGTC TGTTGTGAAC AAGGTCACCC AGAATTACAA GTTTGTGTGG
    GCCTGTTTTA ATAGATACTA TGGTGCACTT TCAAAATTAA AAAGTCAACA CCAAGAAGAC
    CCCAACAGTA CAATACTTAC TGCCAATAAA CCAGCACTCC TTAGATCACT TTTCACTGTT
    GGAGCACTGT GTCGGCATTT TGATTTTGAC CATGAAGATT TTAAGGGCAA CAGTAAGGTT
    AACATTAAGG ATAAAGTACT TGAACTGCTG ATGTATTTTA CAAAGCATTC AGACGAAGAA
    GTACAGACAA AAGCCATTAT TGGCCTGGGA TTTGCATTTA TACAACACCC AAGCTTAATG
    TTTGAACAGG AAGTAAAGAC TCTGTACAAC AGCATTCTTT CAGATAAGAA CTGTTCAGTA
    AACCTAAAAA TCCAGGTGTT AAAAAACCTC CAGACCTACT TGCAGGAGGA GGACACACGT
    ATGCAGCAGG CAGACAGAGA CTGGAAAAAA GTAGCAAAAC AGGAAGACCT GAAAGAAATG
    GGTGATATTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TGTATCTCAA ACAGGTCCTT
    GAGGCTTTCT TTCATACCCA GTCCAATGTA CGCCACTTTG CTCTAAATGT CATTGCACTG
    ACGTTAAACC AGGGTCTCAT CCATCCTGTT CAGTGTGTGC CATACTTAAT TGCTATGGGC
    ACAGATCCAG AGCCTTCTAT GCGAAACAAA GCTGACCAGC AGTTGGTGGA AATAGACAAA
    AAGTATGCTG GTTTCATTCA CATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
    CAACAGGCAA TCAATACCTG CCCAAAGGAT CCTGTAAGAG GCTTTAGACA CGATGAATCA
    TCCAGTGCAT TGTGTTCACA CCTATATTCT ATGATCCGAG GGAACCGTCA ACACAGACGA
    GCATTTCTAA TTTCTTTACT CAATCTCTTC GATGATACTG CAAAAACAGA GGTGAATATG
    CTCTTGTACA TAGCAGACAA TCTAGCCTGT TTTCCTTATC AAACGCAGGA AGAGCCACTG
    TTTATAATGC ATCATATAGA CATTACACTA TCAGTTTCTG GTAGCAACCT ACTACAGTCA
    TTTAAAGAGT CAATGGTGAA AGACAAAAAA AAGGAAAGAA AGCCTTCATC AGATGAGGAG
    ACTGAGAGTG ACAGTAATAG TGGCAGTGAG AGCGAAAGTG AAGAGGAAGC TTCTAAGCCT
    CGGAGGTTAC GAAAACGAGT AGATTCTGAC TCAGATTCTG ATGAAGAAAA TGATATAAAT
    GCTGTGATGA AATGTTTACC AGAAAACTCA GCTCCTTTAA TTGAATTTGC AAATGTGTCT
    CAAGGCATAT TATTACTTTT GATGCTGAAA CAGCATTTAA AGAACCTTTG CGGATTCTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCT GAGTCTGCAA AAGTTTATGA TAAAGCGATA
    AACAGGAAGA CAGGAGTGCA TTTCCATCCA AAACAAACTC TGGATTTTCT ACGGAGTGAT
    ATGGCTAATT CCAAGATCAC TGAAGAAGTG AAGAGGAGTA TAGTAAAACA GTACTTAGAT
    TTCAAGCTCC TTATGGAACA TTTGGATCCT GATGAAGAAG AAGAAGATGG AGAGGTGTCA
    GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AACAATGCAG CTGAGACAGA GGACGATGAA AGTGATGGGG AGGATAGAGG AGGAGGCACT
    TCAGGGTCAT TGAGAAGGTC AAAACGAAAT TCAGACTCTA CGGAGTTGGC TTCACAGATG
    AATGAAAGTG TTGATGTCAT GGATGTCATC GCTATTTGCT GTCCAAAGTA CAAAGATCGA
    CCACAAATTG CAAGAGTAGT ACAGAAAACC AGCAATGGCT TCAGTGTTCA GTGGATGGCA
    GGCTCCTACA GTGGCTCCTG GACTGAGGCT AAGCGCCGTG ATGGCCGCAA ACTGGTGCCT
    TGGGTAGACA CTATTAAAGA GTCAGACATT ATTTACAAAA AAATTGCTCT AACGAGTGCT
    AATAAGCTGA CTAATAAAGT TGTTCAGACT TTACGATCGC TGTATGCCGC CAAGGATGGA
    ACTTCGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa20946
    Clone ID Related Accession (Same CDS sequence) XM_425012.5 , XM_425012.6 , XM_015277519.2 , XM_015277519.1 , XM_015277520.1 , XM_015277520.2
    Accession Version XM_015277519.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8352bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product nipped-B-like protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGATAAGCCT
    CTGAAGAAGC GAAAACAAGA AAACAAACCA CAGGAGGCTG GGGGTGCTGC TGGAGGAAAT
    AGGCCAGCTT CTCAGGAGTC AGGTTCTGCA GGAAATGGGG CAAGGCCAGC ACTGATGGTT
    AGTATTGATC TTCAACAAGC AGGAAGAGCG GACTCTCAGG CAACAATAAC TCAGGAATTT
    GAGGCTATCA AAAAACCTGA AGAGATTAAG CAGTATAATG ATGGATTTGT GTGTATTCCC
    CAAGAAGAGA CTGTTGGAGT TCTTAAACTT AAACCTGAAA ACCACCCTGA GATTTTTAGG
    AAAAAGTCAG ACTTTGAGGT TCAAAAGACA GATATCCAGC AAAATGAAAA CAGACAAATG
    GAGTTTCAGC AAAATGAGAG CAGAAGGTTG GAAAGTAAAT ACAATGACAA TAAACAGGTA
    GAAGCTAAAT ATGACATCAA GCATGACAGT AGACAAATGG AGATGAAACA AAATGAGAAC
    AGACAGGCAG AGTCAAAACA AAATGAGAGC AGACAAATGG AGATGAAACA AAATGAGGGA
    AAACAAAATA ATGGCAAACA AGAAATAAGA CAGAGACCTG AAACACCAAA GCAGAAGAGC
    GAAGGCAGGC CTGAAACACC CAAGCAGAAG AGTGAAGGCA GGCCTGAAAC ACCAAAACAT
    AGGCATGATA ACAGGAGGGA CTCCAGTAAA CCATTGTCAG AAAAGAAAAT TGAATTATCT
    AGGCATAAAC CTGATGTGAA ATCTGATACT TCAAAGGTAA AATCTGATAG TAGATCAGAT
    CCCGTGAAGC AAAGGCCTGA TGGGCGTTCA GTTTCTGATA CTCAGAGGCG TGACCACGAT
    AGCCTTAAAC AGAGATCTGA GGACAGGGAA TCAGAAAGAT ACAAAGGAGA TCCATCCAAG
    ATTAGAAGGA CTGAATTTTT GAGATCCTCA AACAGAAATG AACATGATTC TAAGTATGGT
    ATTAAATCTG ATGGTTCAAA ACCTGAAAAA TTTGAAAGGA AACACAGACA TGACTCTGGT
    GAATCAAGGG AAAGATTTTC TTCTGGGGAT CAGAAGTCAG ATCAAAAGTC AAGACCTGAC
    AGCCCTCGAG TTAAACAGGA TGGCAAAGGA GATTCTAGCA AATCAAGACC GGATAAACCT
    GGTTTTAGAT CACCCAACAG CAAGGATGAA CGAAGGACAG ACAGTAGTAA GAGTAAAGTA
    GAGAGTAATA AAGCACATCC TGACAATAAA GCAGAGTTTC CCAGTTATCT GCTGGGGGCA
    AGATCTGGTG CACTGAAACA TTTTGTCATT CCAAAAATCA AACGTGACAA AGATGGCAAT
    GTCACTCAGG AGTCAAGGAA AACTGAACTT AAAGGTGAAC AGAAGGACAA AGTAGAGAAG
    ATTGGGCTAG TTGAAGACCT AAATAAAGGA GCTAAGCCTG TAGTTGTCCT TCAGAAGCTT
    TCTTTGGACG ATGTACAAAA ATTTATTAAG GATAGAGAAG ATAAATCAAG GGGCTCTTGT
    TTTAAACCTA ACAAGAACAA GTCGTCCAAA TCTAATAAAG GTAGCATAGA TCAGTCAGTG
    CTAAAGGAGT TGCCTCCTGA GCTCCTGGCA GAAATTGAAT CCACCATGCC TCTTTGTGAA
    CGCGTGAAAA TGAACAAGCG CAAACGTAGC ACAGTTAATG AGAAACCAAA ATATGCTGAA
    ATCAGTTCTG ATGAAGACAA TGACAGTGAA GAAGCTTTTG AATCTTCTCG AAAAAGACAT
    AAAAAGGATA GAGATGAAGC TTGGGAGTAC GAAGAGCATG ACAGAAGAAG TTCTGGTGAC
    CACAGGAGGA GTGGTCATTA TCATGAAGGA AGAAGGACTT CTGGTAGTGG CCGTTACCGC
    AATCGCAGTC CCGATGACTC TGATCTGGAT GATTATTCTC CTCCTCCTAG CCTCAGTGAG
    GTGGCAAGAA AAATGAAGAA AAAAGAAAAG CAAAAGAAGA GGAGAGCTTA CGAGCCTAAA
    CTAACACCTG AAGAAATGAT GGATTCTTCA ACCTTCAAAA GATTTATTGC TTCAATAGAG
    AATATTCTGG AAAATCTGGA AGACATGGAT TTTACAACCT TGGGTGATGA TGATGAGATT
    CCACAGGAAT TGCTTTTGGG AAAACATCAG CTTAATGAGT TGGGTAGTGA ATCTGCAAAA
    ATCAAGGCAA TGGGTATTAT GGACAAGCTC TCAACAGATA AAATAGTAAA GGTCCTGAAC
    ATTTTGGAGA AGAATATTCA AGACGGATCA AAGCTTTCTA CATTACTTAA CCACAACAAT
    GACACTGAAG ATGAGGAGAG ATTATGGAGA GATCTCATAA TGGAGAGAGT AACAAAATCT
    GCAGATGCTT GTCTTACTGC TATCAATATT ATGACATCAC CAAATATGCC TAAAGCTGTT
    TATATTGAGG ACGTAATAGA AAGAGTTATT CAGTACACAA AATTTCATTT GCAGAACACT
    CTCTATCCTC AGTATGACCC TGTGTATAGA GTGGATCCTC TTGGTGGTGG TCTTTTAAGT
    TCAAAAGCAA AACGTGCCAA GTGTTCCACC CACAAACAAA GAGTTATAGT GATGTTGTAT
    AACAAAGTTT GTGATATTGT CAGCAGTTTG TCAGAGTTGC TAGAGATACA ACTTCTTACT
    GATACAACTA TTCTTCAGGT ATCGTCTATG GGGATAACAC CTTTCTTTGT AGAAAATGTC
    AGTGAACTAC AGCTATGTGC CATCAAATTA GTGACTGCAG TCTTCTCCAG GTATGAAAAA
    CATAGACAGC TAATACTAGA AGAAATTTTT ACTTCCCTTG CAAGACTACC AACTAGCAAG
    AGGAGCCTGA GAAACTTCAG GTTGAATAGC AGTGACACAG ATGGAGAGCC TATGTATATT
    CAGATGGTAA CAGCACTAGT TCTGCAGCTT ATTCAATGTG TCGTGCACTT ACCATCAGCA
    GAAAAAGATT CTAATTCAGA TGAGGAATTG AACAAAAAAG TGGATCAAGA TGTGCTTATT
    ACTAACTCAT ATGAAACAGC TATGAGAACA GCACAAAATT TTCTTTCTAT TTTTCTTAAG
    AAATGTGGTA GTAAACAAGG AGAAGAAGAT TACAGGCCTC TCTTTGAGAA CTTCATTCAA
    GATCTTCTTT CCACAGTCAA TAAACCAGAA TGGCCAGCTG CAGAGTTGCT GCTTAGCTTA
    CTAGGAAGGT TATTGGTTCA CCAGTTCAGT AATAAATCAA CAGAAATGGC TTTAAGAGTT
    GCGTCACTTG ACTATCTTGG AACCGTAGCT GCAAGACTGA GGAAAGATGC AGTCACTAGT
    AAAATGGATC AAGGATCTAT AGCTAGAATC CTCAAACAGG CCTCAGGAGG AGAAGATGAT
    ATTCAACAGC TACAGAAGGC TTTATTGGAT TATCTGGATG AGAACACTGA AACTGATGCA
    TCGTTAGTGT TTTCTCGGAA GTTTTATATA GCTCAGTGGT TTCGGGATAC AACTATGGAG
    ACAGAGAAGG CAATTAAATC TCAGAAGGAT GAGGATTCAT CTGAAGGAAC TCATCATGCA
    AAAGATGTTG AGACCACAGG ACAAATCATG CACAGAGCAG AAAGCCGCAA GAAGTTTCTC
    CGCAGCATCA TTAAAACTGC TCCATCTCAG TTCAGTACAT TAAAGATTTA TTCTGACACT
    GTGGACTATG AAGATGCATG TTTGATTGTT CGCTACTTGG CCTCTATGAG GCCGTTTGCC
    CAGAGCTTCG ATATTTATTT AACACAGATT CTGCGTGTAC TTGGTGAAAA TGCAATTGCA
    GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGATCC CAGTATTCTA
    GCCAGGCCGG ATATGCAGCG AGGTGTTCAT GGACGGTTAA TGGATAACTC CACCAGTGTA
    CGGGAAGCAG CTGTGGAGCT GCTTGGCCGA TTTGTGCTCT GTCGTCCTCA GCTTGCTGAG
    CAGTATTATG ACATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGAAAAAGA
    GTCATAAAGA TACTTAGGGA TATCTGCATT GAACAACCAA CGTTTCCCAA AATTACAGAG
    ATGTGTGTGA AAATGATTCG GAGAGTCAAT GATGAAGAAG GCATTAAGAA ATTAGTAAAT
    GAGACATTCC AGAAGCTGTG GTTTACTCCA ACTCCACACC ATGACAAAGA AGCAATGACT
    AGGAAAATAC TGAACATCAC TGATGTGGTG GCAGCTTGTA GGGATACAGG GTATGATTGG
    TTTGAGCAGC TTCTTCAAAA TCTATTGAAG TCAGAAGAGG ATGCTTCATA TAAACCTGTG
    AAGAAGGCTT GTACTCAACT TGTTGACAAT TTGGTTGAGC ACATCCTTAA ATATGAGGAG
    TCTTTATCAG ACTCTGACAA CAAAGGAGTG AATTCTGGTC GCTTGGTTGC TTGTATAACC
    ACTCTGTTCT TATTCAGCAA AATCAGGCCC CAGTTAATGG TCAAACATGC CATGACCATG
    CAGCCATATC TGGCAACTAA ATGTAGTACT CAGAATGATT TCATGGTGAT CTGCAATGTT
    GCAAAAATCT TGGAGCTTGT AGTGCCGTTA ATGGAACACC CAAGTGAGAC CTTCCTTGCC
    ACTATTGAGG AAGACCTCAT GAAACTCATC ATCAAATATG GCATGACAGT TGTTCAACAT
    TGTGTGAGCT GCCTTGGGTC TGTTGTGAAC AAGGTCACCC AGAATTACAA GTTTGTGTGG
    GCCTGTTTTA ATAGATACTA TGGTGCACTT TCAAAATTAA AAAGTCAACA CCAAGAAGAC
    CCCAACAGTA CAATACTTAC TGCCAATAAA CCAGCACTCC TTAGATCACT TTTCACTGTT
    GGAGCACTGT GTCGGCATTT TGATTTTGAC CATGAAGATT TTAAGGGCAA CAGTAAGGTT
    AACATTAAGG ATAAAGTACT TGAACTGCTG ATGTATTTTA CAAAGCATTC AGACGAAGAA
    GTACAGACAA AAGCCATTAT TGGCCTGGGA TTTGCATTTA TACAACACCC AAGCTTAATG
    TTTGAACAGG AAGTAAAGAC TCTGTACAAC AGCATTCTTT CAGATAAGAA CTGTTCAGTA
    AACCTAAAAA TCCAGGTGTT AAAAAACCTC CAGACCTACT TGCAGGAGGA GGACACACGT
    ATGCAGCAGG CAGACAGAGA CTGGAAAAAA GTAGCAAAAC AGGAAGACCT GAAAGAAATG
    GGTGATATTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TGTATCTCAA ACAGGTCCTT
    GAGGCTTTCT TTCATACCCA GTCCAATGTA CGCCACTTTG CTCTAAATGT CATTGCACTG
    ACGTTAAACC AGGGTCTCAT CCATCCTGTT CAGTGTGTGC CATACTTAAT TGCTATGGGC
    ACAGATCCAG AGCCTTCTAT GCGAAACAAA GCTGACCAGC AGTTGGTGGA AATAGACAAA
    AAGTATGCTG GTTTCATTCA CATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
    CAACAGGCAA TCAATACCTG CCCAAAGGAT CCTGTAAGAG GCTTTAGACA CGATGAATCA
    TCCAGTGCAT TGTGTTCACA CCTATATTCT ATGATCCGAG GGAACCGTCA ACACAGACGA
    GCATTTCTAA TTTCTTTACT CAATCTCTTC GATGATACTG CAAAAACAGA GGTGAATATG
    CTCTTGTACA TAGCAGACAA TCTAGCCTGT TTTCCTTATC AAACGCAGGA AGAGCCACTG
    TTTATAATGC ATCATATAGA CATTACACTA TCAGTTTCTG GTAGCAACCT ACTACAGTCA
    TTTAAAGAGT CAATGGTGAA AGACAAAAAA AAGGAAAGAA AGCCTTCATC AGATGAGGAG
    ACTGAGAGTG ACAGTAATAG TGGCAGTGAG AGCGAAAGTG AAGAGGAAGC TTCTAAGCCT
    CGGAGGTTAC GAAAACGAGT AGATTCTGAC TCAGATTCTG ATGAAGAAAA TGATATAAAT
    GCTGTGATGA AATGTTTACC AGAAAACTCA GCTCCTTTAA TTGAATTTGC AAATGTGTCT
    CAAGGCATAT TATTACTTTT GATGCTGAAA CAGCATTTAA AGAACCTTTG CGGATTCTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCT GAGTCTGCAA AAGTTTATGA TAAAGCGATA
    AACAGGAAGA CAGGAGTGCA TTTCCATCCA AAACAAACTC TGGATTTTCT ACGGAGTGAT
    ATGGCTAATT CCAAGATCAC TGAAGAAGTG AAGAGGAGTA TAGTAAAACA GTACTTAGAT
    TTCAAGCTCC TTATGGAACA TTTGGATCCT GATGAAGAAG AAGAAGATGG AGAGGTGTCA
    GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AACAATGCAG CTGAGACAGA GGACGATGAA AGTGATGGGG AGGATAGAGG AGGAGGCACT
    TCAGGGTCAT TGAGAAGGTC AAAACGAAAT TCAGACTCTA CGGAGTTGGC TTCACAGATG
    AATGAAAGTG TTGATGTCAT GGATGTCATC GCTATTTGCT GTCCAAAGTA CAAAGATCGA
    CCACAAATTG CAAGAGTAGT ACAGAAAACC AGCAATGGCT TCAGTGTTCA GTGGATGGCA
    GGCTCCTACA GTGGCTCCTG GACTGAGGCT AAGCGCCGTG ATGGCCGCAA ACTGGTGCCT
    TGGGTAGACA CTATTAAAGA GTCAGACATT ATTTACAAAA AAATTGCTCT AACGAGTGCT
    AATAAGCTGA CTAATAAAGT TGTTCAGACT TTACGATCGC TGTATGCCGC CAAGGATGGA
    ACTTCGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133005.1
    CDS3249..11600
    Translation

    Target ORF information:

    RefSeq Version XM_015277519.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277519.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGATAAGCCT
    CTGAAGAAGC GAAAACAAGA AAACAAACCA CAGGAGGCTG GGGGTGCTGC TGGAGGAAAT
    AGGCCAGCTT CTCAGGAGTC AGGTTCTGCA GGAAATGGGG CAAGGCCAGC ACTGATGGTT
    AGTATTGATC TTCAACAAGC AGGAAGAGCG GACTCTCAGG CAACAATAAC TCAGGAATTT
    GAGGCTATCA AAAAACCTGA AGAGATTAAG CAGTATAATG ATGGATTTGT GTGTATTCCC
    CAAGAAGAGA CTGTTGGAGT TCTTAAACTT AAACCTGAAA ACCACCCTGA GATTTTTAGG
    AAAAAGTCAG ACTTTGAGGT TCAAAAGACA GATATCCAGC AAAATGAAAA CAGACAAATG
    GAGTTTCAGC AAAATGAGAG CAGAAGGTTG GAAAGTAAAT ACAATGACAA TAAACAGGTA
    GAAGCTAAAT ATGACATCAA GCATGACAGT AGACAAATGG AGATGAAACA AAATGAGAAC
    AGACAGGCAG AGTCAAAACA AAATGAGAGC AGACAAATGG AGATGAAACA AAATGAGGGA
    AAACAAAATA ATGGCAAACA AGAAATAAGA CAGAGACCTG AAACACCAAA GCAGAAGAGC
    GAAGGCAGGC CTGAAACACC CAAGCAGAAG AGTGAAGGCA GGCCTGAAAC ACCAAAACAT
    AGGCATGATA ACAGGAGGGA CTCCAGTAAA CCATTGTCAG AAAAGAAAAT TGAATTATCT
    AGGCATAAAC CTGATGTGAA ATCTGATACT TCAAAGGTAA AATCTGATAG TAGATCAGAT
    CCCGTGAAGC AAAGGCCTGA TGGGCGTTCA GTTTCTGATA CTCAGAGGCG TGACCACGAT
    AGCCTTAAAC AGAGATCTGA GGACAGGGAA TCAGAAAGAT ACAAAGGAGA TCCATCCAAG
    ATTAGAAGGA CTGAATTTTT GAGATCCTCA AACAGAAATG AACATGATTC TAAGTATGGT
    ATTAAATCTG ATGGTTCAAA ACCTGAAAAA TTTGAAAGGA AACACAGACA TGACTCTGGT
    GAATCAAGGG AAAGATTTTC TTCTGGGGAT CAGAAGTCAG ATCAAAAGTC AAGACCTGAC
    AGCCCTCGAG TTAAACAGGA TGGCAAAGGA GATTCTAGCA AATCAAGACC GGATAAACCT
    GGTTTTAGAT CACCCAACAG CAAGGATGAA CGAAGGACAG ACAGTAGTAA GAGTAAAGTA
    GAGAGTAATA AAGCACATCC TGACAATAAA GCAGAGTTTC CCAGTTATCT GCTGGGGGCA
    AGATCTGGTG CACTGAAACA TTTTGTCATT CCAAAAATCA AACGTGACAA AGATGGCAAT
    GTCACTCAGG AGTCAAGGAA AACTGAACTT AAAGGTGAAC AGAAGGACAA AGTAGAGAAG
    ATTGGGCTAG TTGAAGACCT AAATAAAGGA GCTAAGCCTG TAGTTGTCCT TCAGAAGCTT
    TCTTTGGACG ATGTACAAAA ATTTATTAAG GATAGAGAAG ATAAATCAAG GGGCTCTTGT
    TTTAAACCTA ACAAGAACAA GTCGTCCAAA TCTAATAAAG GTAGCATAGA TCAGTCAGTG
    CTAAAGGAGT TGCCTCCTGA GCTCCTGGCA GAAATTGAAT CCACCATGCC TCTTTGTGAA
    CGCGTGAAAA TGAACAAGCG CAAACGTAGC ACAGTTAATG AGAAACCAAA ATATGCTGAA
    ATCAGTTCTG ATGAAGACAA TGACAGTGAA GAAGCTTTTG AATCTTCTCG AAAAAGACAT
    AAAAAGGATA GAGATGAAGC TTGGGAGTAC GAAGAGCATG ACAGAAGAAG TTCTGGTGAC
    CACAGGAGGA GTGGTCATTA TCATGAAGGA AGAAGGACTT CTGGTAGTGG CCGTTACCGC
    AATCGCAGTC CCGATGACTC TGATCTGGAT GATTATTCTC CTCCTCCTAG CCTCAGTGAG
    GTGGCAAGAA AAATGAAGAA AAAAGAAAAG CAAAAGAAGA GGAGAGCTTA CGAGCCTAAA
    CTAACACCTG AAGAAATGAT GGATTCTTCA ACCTTCAAAA GATTTATTGC TTCAATAGAG
    AATATTCTGG AAAATCTGGA AGACATGGAT TTTACAACCT TGGGTGATGA TGATGAGATT
    CCACAGGAAT TGCTTTTGGG AAAACATCAG CTTAATGAGT TGGGTAGTGA ATCTGCAAAA
    ATCAAGGCAA TGGGTATTAT GGACAAGCTC TCAACAGATA AAATAGTAAA GGTCCTGAAC
    ATTTTGGAGA AGAATATTCA AGACGGATCA AAGCTTTCTA CATTACTTAA CCACAACAAT
    GACACTGAAG ATGAGGAGAG ATTATGGAGA GATCTCATAA TGGAGAGAGT AACAAAATCT
    GCAGATGCTT GTCTTACTGC TATCAATATT ATGACATCAC CAAATATGCC TAAAGCTGTT
    TATATTGAGG ACGTAATAGA AAGAGTTATT CAGTACACAA AATTTCATTT GCAGAACACT
    CTCTATCCTC AGTATGACCC TGTGTATAGA GTGGATCCTC TTGGTGGTGG TCTTTTAAGT
    TCAAAAGCAA AACGTGCCAA GTGTTCCACC CACAAACAAA GAGTTATAGT GATGTTGTAT
    AACAAAGTTT GTGATATTGT CAGCAGTTTG TCAGAGTTGC TAGAGATACA ACTTCTTACT
    GATACAACTA TTCTTCAGGT ATCGTCTATG GGGATAACAC CTTTCTTTGT AGAAAATGTC
    AGTGAACTAC AGCTATGTGC CATCAAATTA GTGACTGCAG TCTTCTCCAG GTATGAAAAA
    CATAGACAGC TAATACTAGA AGAAATTTTT ACTTCCCTTG CAAGACTACC AACTAGCAAG
    AGGAGCCTGA GAAACTTCAG GTTGAATAGC AGTGACACAG ATGGAGAGCC TATGTATATT
    CAGATGGTAA CAGCACTAGT TCTGCAGCTT ATTCAATGTG TCGTGCACTT ACCATCAGCA
    GAAAAAGATT CTAATTCAGA TGAGGAATTG AACAAAAAAG TGGATCAAGA TGTGCTTATT
    ACTAACTCAT ATGAAACAGC TATGAGAACA GCACAAAATT TTCTTTCTAT TTTTCTTAAG
    AAATGTGGTA GTAAACAAGG AGAAGAAGAT TACAGGCCTC TCTTTGAGAA CTTCATTCAA
    GATCTTCTTT CCACAGTCAA TAAACCAGAA TGGCCAGCTG CAGAGTTGCT GCTTAGCTTA
    CTAGGAAGGT TATTGGTTCA CCAGTTCAGT AATAAATCAA CAGAAATGGC TTTAAGAGTT
    GCGTCACTTG ACTATCTTGG AACCGTAGCT GCAAGACTGA GGAAAGATGC AGTCACTAGT
    AAAATGGATC AAGGATCTAT AGCTAGAATC CTCAAACAGG CCTCAGGAGG AGAAGATGAT
    ATTCAACAGC TACAGAAGGC TTTATTGGAT TATCTGGATG AGAACACTGA AACTGATGCA
    TCGTTAGTGT TTTCTCGGAA GTTTTATATA GCTCAGTGGT TTCGGGATAC AACTATGGAG
    ACAGAGAAGG CAATTAAATC TCAGAAGGAT GAGGATTCAT CTGAAGGAAC TCATCATGCA
    AAAGATGTTG AGACCACAGG ACAAATCATG CACAGAGCAG AAAGCCGCAA GAAGTTTCTC
    CGCAGCATCA TTAAAACTGC TCCATCTCAG TTCAGTACAT TAAAGATTTA TTCTGACACT
    GTGGACTATG AAGATGCATG TTTGATTGTT CGCTACTTGG CCTCTATGAG GCCGTTTGCC
    CAGAGCTTCG ATATTTATTT AACACAGATT CTGCGTGTAC TTGGTGAAAA TGCAATTGCA
    GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGATCC CAGTATTCTA
    GCCAGGCCGG ATATGCAGCG AGGTGTTCAT GGACGGTTAA TGGATAACTC CACCAGTGTA
    CGGGAAGCAG CTGTGGAGCT GCTTGGCCGA TTTGTGCTCT GTCGTCCTCA GCTTGCTGAG
    CAGTATTATG ACATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGAAAAAGA
    GTCATAAAGA TACTTAGGGA TATCTGCATT GAACAACCAA CGTTTCCCAA AATTACAGAG
    ATGTGTGTGA AAATGATTCG GAGAGTCAAT GATGAAGAAG GCATTAAGAA ATTAGTAAAT
    GAGACATTCC AGAAGCTGTG GTTTACTCCA ACTCCACACC ATGACAAAGA AGCAATGACT
    AGGAAAATAC TGAACATCAC TGATGTGGTG GCAGCTTGTA GGGATACAGG GTATGATTGG
    TTTGAGCAGC TTCTTCAAAA TCTATTGAAG TCAGAAGAGG ATGCTTCATA TAAACCTGTG
    AAGAAGGCTT GTACTCAACT TGTTGACAAT TTGGTTGAGC ACATCCTTAA ATATGAGGAG
    TCTTTATCAG ACTCTGACAA CAAAGGAGTG AATTCTGGTC GCTTGGTTGC TTGTATAACC
    ACTCTGTTCT TATTCAGCAA AATCAGGCCC CAGTTAATGG TCAAACATGC CATGACCATG
    CAGCCATATC TGGCAACTAA ATGTAGTACT CAGAATGATT TCATGGTGAT CTGCAATGTT
    GCAAAAATCT TGGAGCTTGT AGTGCCGTTA ATGGAACACC CAAGTGAGAC CTTCCTTGCC
    ACTATTGAGG AAGACCTCAT GAAACTCATC ATCAAATATG GCATGACAGT TGTTCAACAT
    TGTGTGAGCT GCCTTGGGTC TGTTGTGAAC AAGGTCACCC AGAATTACAA GTTTGTGTGG
    GCCTGTTTTA ATAGATACTA TGGTGCACTT TCAAAATTAA AAAGTCAACA CCAAGAAGAC
    CCCAACAGTA CAATACTTAC TGCCAATAAA CCAGCACTCC TTAGATCACT TTTCACTGTT
    GGAGCACTGT GTCGGCATTT TGATTTTGAC CATGAAGATT TTAAGGGCAA CAGTAAGGTT
    AACATTAAGG ATAAAGTACT TGAACTGCTG ATGTATTTTA CAAAGCATTC AGACGAAGAA
    GTACAGACAA AAGCCATTAT TGGCCTGGGA TTTGCATTTA TACAACACCC AAGCTTAATG
    TTTGAACAGG AAGTAAAGAC TCTGTACAAC AGCATTCTTT CAGATAAGAA CTGTTCAGTA
    AACCTAAAAA TCCAGGTGTT AAAAAACCTC CAGACCTACT TGCAGGAGGA GGACACACGT
    ATGCAGCAGG CAGACAGAGA CTGGAAAAAA GTAGCAAAAC AGGAAGACCT GAAAGAAATG
    GGTGATATTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TGTATCTCAA ACAGGTCCTT
    GAGGCTTTCT TTCATACCCA GTCCAATGTA CGCCACTTTG CTCTAAATGT CATTGCACTG
    ACGTTAAACC AGGGTCTCAT CCATCCTGTT CAGTGTGTGC CATACTTAAT TGCTATGGGC
    ACAGATCCAG AGCCTTCTAT GCGAAACAAA GCTGACCAGC AGTTGGTGGA AATAGACAAA
    AAGTATGCTG GTTTCATTCA CATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
    CAACAGGCAA TCAATACCTG CCCAAAGGAT CCTGTAAGAG GCTTTAGACA CGATGAATCA
    TCCAGTGCAT TGTGTTCACA CCTATATTCT ATGATCCGAG GGAACCGTCA ACACAGACGA
    GCATTTCTAA TTTCTTTACT CAATCTCTTC GATGATACTG CAAAAACAGA GGTGAATATG
    CTCTTGTACA TAGCAGACAA TCTAGCCTGT TTTCCTTATC AAACGCAGGA AGAGCCACTG
    TTTATAATGC ATCATATAGA CATTACACTA TCAGTTTCTG GTAGCAACCT ACTACAGTCA
    TTTAAAGAGT CAATGGTGAA AGACAAAAAA AAGGAAAGAA AGCCTTCATC AGATGAGGAG
    ACTGAGAGTG ACAGTAATAG TGGCAGTGAG AGCGAAAGTG AAGAGGAAGC TTCTAAGCCT
    CGGAGGTTAC GAAAACGAGT AGATTCTGAC TCAGATTCTG ATGAAGAAAA TGATATAAAT
    GCTGTGATGA AATGTTTACC AGAAAACTCA GCTCCTTTAA TTGAATTTGC AAATGTGTCT
    CAAGGCATAT TATTACTTTT GATGCTGAAA CAGCATTTAA AGAACCTTTG CGGATTCTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCT GAGTCTGCAA AAGTTTATGA TAAAGCGATA
    AACAGGAAGA CAGGAGTGCA TTTCCATCCA AAACAAACTC TGGATTTTCT ACGGAGTGAT
    ATGGCTAATT CCAAGATCAC TGAAGAAGTG AAGAGGAGTA TAGTAAAACA GTACTTAGAT
    TTCAAGCTCC TTATGGAACA TTTGGATCCT GATGAAGAAG AAGAAGATGG AGAGGTGTCA
    GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AACAATGCAG CTGAGACAGA GGACGATGAA AGTGATGGGG AGGATAGAGG AGGAGGCACT
    TCAGGGTCAT TGAGAAGGTC AAAACGAAAT TCAGACTCTA CGGAGTTGGC TTCACAGATG
    AATGAAAGTG TTGATGTCAT GGATGTCATC GCTATTTGCT GTCCAAAGTA CAAAGATCGA
    CCACAAATTG CAAGAGTAGT ACAGAAAACC AGCAATGGCT TCAGTGTTCA GTGGATGGCA
    GGCTCCTACA GTGGCTCCTG GACTGAGGCT AAGCGCCGTG ATGGCCGCAA ACTGGTGCCT
    TGGGTAGACA CTATTAAAGA GTCAGACATT ATTTACAAAA AAATTGCTCT AACGAGTGCT
    AATAAGCTGA CTAATAAAGT TGTTCAGACT TTACGATCGC TGTATGCCGC CAAGGATGGA
    ACTTCGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa20946
    Clone ID Related Accession (Same CDS sequence) XM_425012.5 , XM_425012.6 , XM_015277519.2 , XM_015277519.1 , XM_015277520.1 , XM_015277520.2
    Accession Version XM_015277520.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8352bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product nipped-B-like protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGATAAGCCT
    CTGAAGAAGC GAAAACAAGA AAACAAACCA CAGGAGGCTG GGGGTGCTGC TGGAGGAAAT
    AGGCCAGCTT CTCAGGAGTC AGGTTCTGCA GGAAATGGGG CAAGGCCAGC ACTGATGGTT
    AGTATTGATC TTCAACAAGC AGGAAGAGCG GACTCTCAGG CAACAATAAC TCAGGAATTT
    GAGGCTATCA AAAAACCTGA AGAGATTAAG CAGTATAATG ATGGATTTGT GTGTATTCCC
    CAAGAAGAGA CTGTTGGAGT TCTTAAACTT AAACCTGAAA ACCACCCTGA GATTTTTAGG
    AAAAAGTCAG ACTTTGAGGT TCAAAAGACA GATATCCAGC AAAATGAAAA CAGACAAATG
    GAGTTTCAGC AAAATGAGAG CAGAAGGTTG GAAAGTAAAT ACAATGACAA TAAACAGGTA
    GAAGCTAAAT ATGACATCAA GCATGACAGT AGACAAATGG AGATGAAACA AAATGAGAAC
    AGACAGGCAG AGTCAAAACA AAATGAGAGC AGACAAATGG AGATGAAACA AAATGAGGGA
    AAACAAAATA ATGGCAAACA AGAAATAAGA CAGAGACCTG AAACACCAAA GCAGAAGAGC
    GAAGGCAGGC CTGAAACACC CAAGCAGAAG AGTGAAGGCA GGCCTGAAAC ACCAAAACAT
    AGGCATGATA ACAGGAGGGA CTCCAGTAAA CCATTGTCAG AAAAGAAAAT TGAATTATCT
    AGGCATAAAC CTGATGTGAA ATCTGATACT TCAAAGGTAA AATCTGATAG TAGATCAGAT
    CCCGTGAAGC AAAGGCCTGA TGGGCGTTCA GTTTCTGATA CTCAGAGGCG TGACCACGAT
    AGCCTTAAAC AGAGATCTGA GGACAGGGAA TCAGAAAGAT ACAAAGGAGA TCCATCCAAG
    ATTAGAAGGA CTGAATTTTT GAGATCCTCA AACAGAAATG AACATGATTC TAAGTATGGT
    ATTAAATCTG ATGGTTCAAA ACCTGAAAAA TTTGAAAGGA AACACAGACA TGACTCTGGT
    GAATCAAGGG AAAGATTTTC TTCTGGGGAT CAGAAGTCAG ATCAAAAGTC AAGACCTGAC
    AGCCCTCGAG TTAAACAGGA TGGCAAAGGA GATTCTAGCA AATCAAGACC GGATAAACCT
    GGTTTTAGAT CACCCAACAG CAAGGATGAA CGAAGGACAG ACAGTAGTAA GAGTAAAGTA
    GAGAGTAATA AAGCACATCC TGACAATAAA GCAGAGTTTC CCAGTTATCT GCTGGGGGCA
    AGATCTGGTG CACTGAAACA TTTTGTCATT CCAAAAATCA AACGTGACAA AGATGGCAAT
    GTCACTCAGG AGTCAAGGAA AACTGAACTT AAAGGTGAAC AGAAGGACAA AGTAGAGAAG
    ATTGGGCTAG TTGAAGACCT AAATAAAGGA GCTAAGCCTG TAGTTGTCCT TCAGAAGCTT
    TCTTTGGACG ATGTACAAAA ATTTATTAAG GATAGAGAAG ATAAATCAAG GGGCTCTTGT
    TTTAAACCTA ACAAGAACAA GTCGTCCAAA TCTAATAAAG GTAGCATAGA TCAGTCAGTG
    CTAAAGGAGT TGCCTCCTGA GCTCCTGGCA GAAATTGAAT CCACCATGCC TCTTTGTGAA
    CGCGTGAAAA TGAACAAGCG CAAACGTAGC ACAGTTAATG AGAAACCAAA ATATGCTGAA
    ATCAGTTCTG ATGAAGACAA TGACAGTGAA GAAGCTTTTG AATCTTCTCG AAAAAGACAT
    AAAAAGGATA GAGATGAAGC TTGGGAGTAC GAAGAGCATG ACAGAAGAAG TTCTGGTGAC
    CACAGGAGGA GTGGTCATTA TCATGAAGGA AGAAGGACTT CTGGTAGTGG CCGTTACCGC
    AATCGCAGTC CCGATGACTC TGATCTGGAT GATTATTCTC CTCCTCCTAG CCTCAGTGAG
    GTGGCAAGAA AAATGAAGAA AAAAGAAAAG CAAAAGAAGA GGAGAGCTTA CGAGCCTAAA
    CTAACACCTG AAGAAATGAT GGATTCTTCA ACCTTCAAAA GATTTATTGC TTCAATAGAG
    AATATTCTGG AAAATCTGGA AGACATGGAT TTTACAACCT TGGGTGATGA TGATGAGATT
    CCACAGGAAT TGCTTTTGGG AAAACATCAG CTTAATGAGT TGGGTAGTGA ATCTGCAAAA
    ATCAAGGCAA TGGGTATTAT GGACAAGCTC TCAACAGATA AAATAGTAAA GGTCCTGAAC
    ATTTTGGAGA AGAATATTCA AGACGGATCA AAGCTTTCTA CATTACTTAA CCACAACAAT
    GACACTGAAG ATGAGGAGAG ATTATGGAGA GATCTCATAA TGGAGAGAGT AACAAAATCT
    GCAGATGCTT GTCTTACTGC TATCAATATT ATGACATCAC CAAATATGCC TAAAGCTGTT
    TATATTGAGG ACGTAATAGA AAGAGTTATT CAGTACACAA AATTTCATTT GCAGAACACT
    CTCTATCCTC AGTATGACCC TGTGTATAGA GTGGATCCTC TTGGTGGTGG TCTTTTAAGT
    TCAAAAGCAA AACGTGCCAA GTGTTCCACC CACAAACAAA GAGTTATAGT GATGTTGTAT
    AACAAAGTTT GTGATATTGT CAGCAGTTTG TCAGAGTTGC TAGAGATACA ACTTCTTACT
    GATACAACTA TTCTTCAGGT ATCGTCTATG GGGATAACAC CTTTCTTTGT AGAAAATGTC
    AGTGAACTAC AGCTATGTGC CATCAAATTA GTGACTGCAG TCTTCTCCAG GTATGAAAAA
    CATAGACAGC TAATACTAGA AGAAATTTTT ACTTCCCTTG CAAGACTACC AACTAGCAAG
    AGGAGCCTGA GAAACTTCAG GTTGAATAGC AGTGACACAG ATGGAGAGCC TATGTATATT
    CAGATGGTAA CAGCACTAGT TCTGCAGCTT ATTCAATGTG TCGTGCACTT ACCATCAGCA
    GAAAAAGATT CTAATTCAGA TGAGGAATTG AACAAAAAAG TGGATCAAGA TGTGCTTATT
    ACTAACTCAT ATGAAACAGC TATGAGAACA GCACAAAATT TTCTTTCTAT TTTTCTTAAG
    AAATGTGGTA GTAAACAAGG AGAAGAAGAT TACAGGCCTC TCTTTGAGAA CTTCATTCAA
    GATCTTCTTT CCACAGTCAA TAAACCAGAA TGGCCAGCTG CAGAGTTGCT GCTTAGCTTA
    CTAGGAAGGT TATTGGTTCA CCAGTTCAGT AATAAATCAA CAGAAATGGC TTTAAGAGTT
    GCGTCACTTG ACTATCTTGG AACCGTAGCT GCAAGACTGA GGAAAGATGC AGTCACTAGT
    AAAATGGATC AAGGATCTAT AGCTAGAATC CTCAAACAGG CCTCAGGAGG AGAAGATGAT
    ATTCAACAGC TACAGAAGGC TTTATTGGAT TATCTGGATG AGAACACTGA AACTGATGCA
    TCGTTAGTGT TTTCTCGGAA GTTTTATATA GCTCAGTGGT TTCGGGATAC AACTATGGAG
    ACAGAGAAGG CAATTAAATC TCAGAAGGAT GAGGATTCAT CTGAAGGAAC TCATCATGCA
    AAAGATGTTG AGACCACAGG ACAAATCATG CACAGAGCAG AAAGCCGCAA GAAGTTTCTC
    CGCAGCATCA TTAAAACTGC TCCATCTCAG TTCAGTACAT TAAAGATTTA TTCTGACACT
    GTGGACTATG AAGATGCATG TTTGATTGTT CGCTACTTGG CCTCTATGAG GCCGTTTGCC
    CAGAGCTTCG ATATTTATTT AACACAGATT CTGCGTGTAC TTGGTGAAAA TGCAATTGCA
    GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGATCC CAGTATTCTA
    GCCAGGCCGG ATATGCAGCG AGGTGTTCAT GGACGGTTAA TGGATAACTC CACCAGTGTA
    CGGGAAGCAG CTGTGGAGCT GCTTGGCCGA TTTGTGCTCT GTCGTCCTCA GCTTGCTGAG
    CAGTATTATG ACATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGAAAAAGA
    GTCATAAAGA TACTTAGGGA TATCTGCATT GAACAACCAA CGTTTCCCAA AATTACAGAG
    ATGTGTGTGA AAATGATTCG GAGAGTCAAT GATGAAGAAG GCATTAAGAA ATTAGTAAAT
    GAGACATTCC AGAAGCTGTG GTTTACTCCA ACTCCACACC ATGACAAAGA AGCAATGACT
    AGGAAAATAC TGAACATCAC TGATGTGGTG GCAGCTTGTA GGGATACAGG GTATGATTGG
    TTTGAGCAGC TTCTTCAAAA TCTATTGAAG TCAGAAGAGG ATGCTTCATA TAAACCTGTG
    AAGAAGGCTT GTACTCAACT TGTTGACAAT TTGGTTGAGC ACATCCTTAA ATATGAGGAG
    TCTTTATCAG ACTCTGACAA CAAAGGAGTG AATTCTGGTC GCTTGGTTGC TTGTATAACC
    ACTCTGTTCT TATTCAGCAA AATCAGGCCC CAGTTAATGG TCAAACATGC CATGACCATG
    CAGCCATATC TGGCAACTAA ATGTAGTACT CAGAATGATT TCATGGTGAT CTGCAATGTT
    GCAAAAATCT TGGAGCTTGT AGTGCCGTTA ATGGAACACC CAAGTGAGAC CTTCCTTGCC
    ACTATTGAGG AAGACCTCAT GAAACTCATC ATCAAATATG GCATGACAGT TGTTCAACAT
    TGTGTGAGCT GCCTTGGGTC TGTTGTGAAC AAGGTCACCC AGAATTACAA GTTTGTGTGG
    GCCTGTTTTA ATAGATACTA TGGTGCACTT TCAAAATTAA AAAGTCAACA CCAAGAAGAC
    CCCAACAGTA CAATACTTAC TGCCAATAAA CCAGCACTCC TTAGATCACT TTTCACTGTT
    GGAGCACTGT GTCGGCATTT TGATTTTGAC CATGAAGATT TTAAGGGCAA CAGTAAGGTT
    AACATTAAGG ATAAAGTACT TGAACTGCTG ATGTATTTTA CAAAGCATTC AGACGAAGAA
    GTACAGACAA AAGCCATTAT TGGCCTGGGA TTTGCATTTA TACAACACCC AAGCTTAATG
    TTTGAACAGG AAGTAAAGAC TCTGTACAAC AGCATTCTTT CAGATAAGAA CTGTTCAGTA
    AACCTAAAAA TCCAGGTGTT AAAAAACCTC CAGACCTACT TGCAGGAGGA GGACACACGT
    ATGCAGCAGG CAGACAGAGA CTGGAAAAAA GTAGCAAAAC AGGAAGACCT GAAAGAAATG
    GGTGATATTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TGTATCTCAA ACAGGTCCTT
    GAGGCTTTCT TTCATACCCA GTCCAATGTA CGCCACTTTG CTCTAAATGT CATTGCACTG
    ACGTTAAACC AGGGTCTCAT CCATCCTGTT CAGTGTGTGC CATACTTAAT TGCTATGGGC
    ACAGATCCAG AGCCTTCTAT GCGAAACAAA GCTGACCAGC AGTTGGTGGA AATAGACAAA
    AAGTATGCTG GTTTCATTCA CATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
    CAACAGGCAA TCAATACCTG CCCAAAGGAT CCTGTAAGAG GCTTTAGACA CGATGAATCA
    TCCAGTGCAT TGTGTTCACA CCTATATTCT ATGATCCGAG GGAACCGTCA ACACAGACGA
    GCATTTCTAA TTTCTTTACT CAATCTCTTC GATGATACTG CAAAAACAGA GGTGAATATG
    CTCTTGTACA TAGCAGACAA TCTAGCCTGT TTTCCTTATC AAACGCAGGA AGAGCCACTG
    TTTATAATGC ATCATATAGA CATTACACTA TCAGTTTCTG GTAGCAACCT ACTACAGTCA
    TTTAAAGAGT CAATGGTGAA AGACAAAAAA AAGGAAAGAA AGCCTTCATC AGATGAGGAG
    ACTGAGAGTG ACAGTAATAG TGGCAGTGAG AGCGAAAGTG AAGAGGAAGC TTCTAAGCCT
    CGGAGGTTAC GAAAACGAGT AGATTCTGAC TCAGATTCTG ATGAAGAAAA TGATATAAAT
    GCTGTGATGA AATGTTTACC AGAAAACTCA GCTCCTTTAA TTGAATTTGC AAATGTGTCT
    CAAGGCATAT TATTACTTTT GATGCTGAAA CAGCATTTAA AGAACCTTTG CGGATTCTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCT GAGTCTGCAA AAGTTTATGA TAAAGCGATA
    AACAGGAAGA CAGGAGTGCA TTTCCATCCA AAACAAACTC TGGATTTTCT ACGGAGTGAT
    ATGGCTAATT CCAAGATCAC TGAAGAAGTG AAGAGGAGTA TAGTAAAACA GTACTTAGAT
    TTCAAGCTCC TTATGGAACA TTTGGATCCT GATGAAGAAG AAGAAGATGG AGAGGTGTCA
    GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AACAATGCAG CTGAGACAGA GGACGATGAA AGTGATGGGG AGGATAGAGG AGGAGGCACT
    TCAGGGTCAT TGAGAAGGTC AAAACGAAAT TCAGACTCTA CGGAGTTGGC TTCACAGATG
    AATGAAAGTG TTGATGTCAT GGATGTCATC GCTATTTGCT GTCCAAAGTA CAAAGATCGA
    CCACAAATTG CAAGAGTAGT ACAGAAAACC AGCAATGGCT TCAGTGTTCA GTGGATGGCA
    GGCTCCTACA GTGGCTCCTG GACTGAGGCT AAGCGCCGTG ATGGCCGCAA ACTGGTGCCT
    TGGGTAGACA CTATTAAAGA GTCAGACATT ATTTACAAAA AAATTGCTCT AACGAGTGCT
    AATAAGCTGA CTAATAAAGT TGTTCAGACT TTACGATCGC TGTATGCCGC CAAGGATGGA
    ACTTCGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133006.1
    CDS401..8752
    Translation

    Target ORF information:

    RefSeq Version XM_015277520.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277520.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGATAAGCCT
    CTGAAGAAGC GAAAACAAGA AAACAAACCA CAGGAGGCTG GGGGTGCTGC TGGAGGAAAT
    AGGCCAGCTT CTCAGGAGTC AGGTTCTGCA GGAAATGGGG CAAGGCCAGC ACTGATGGTT
    AGTATTGATC TTCAACAAGC AGGAAGAGCG GACTCTCAGG CAACAATAAC TCAGGAATTT
    GAGGCTATCA AAAAACCTGA AGAGATTAAG CAGTATAATG ATGGATTTGT GTGTATTCCC
    CAAGAAGAGA CTGTTGGAGT TCTTAAACTT AAACCTGAAA ACCACCCTGA GATTTTTAGG
    AAAAAGTCAG ACTTTGAGGT TCAAAAGACA GATATCCAGC AAAATGAAAA CAGACAAATG
    GAGTTTCAGC AAAATGAGAG CAGAAGGTTG GAAAGTAAAT ACAATGACAA TAAACAGGTA
    GAAGCTAAAT ATGACATCAA GCATGACAGT AGACAAATGG AGATGAAACA AAATGAGAAC
    AGACAGGCAG AGTCAAAACA AAATGAGAGC AGACAAATGG AGATGAAACA AAATGAGGGA
    AAACAAAATA ATGGCAAACA AGAAATAAGA CAGAGACCTG AAACACCAAA GCAGAAGAGC
    GAAGGCAGGC CTGAAACACC CAAGCAGAAG AGTGAAGGCA GGCCTGAAAC ACCAAAACAT
    AGGCATGATA ACAGGAGGGA CTCCAGTAAA CCATTGTCAG AAAAGAAAAT TGAATTATCT
    AGGCATAAAC CTGATGTGAA ATCTGATACT TCAAAGGTAA AATCTGATAG TAGATCAGAT
    CCCGTGAAGC AAAGGCCTGA TGGGCGTTCA GTTTCTGATA CTCAGAGGCG TGACCACGAT
    AGCCTTAAAC AGAGATCTGA GGACAGGGAA TCAGAAAGAT ACAAAGGAGA TCCATCCAAG
    ATTAGAAGGA CTGAATTTTT GAGATCCTCA AACAGAAATG AACATGATTC TAAGTATGGT
    ATTAAATCTG ATGGTTCAAA ACCTGAAAAA TTTGAAAGGA AACACAGACA TGACTCTGGT
    GAATCAAGGG AAAGATTTTC TTCTGGGGAT CAGAAGTCAG ATCAAAAGTC AAGACCTGAC
    AGCCCTCGAG TTAAACAGGA TGGCAAAGGA GATTCTAGCA AATCAAGACC GGATAAACCT
    GGTTTTAGAT CACCCAACAG CAAGGATGAA CGAAGGACAG ACAGTAGTAA GAGTAAAGTA
    GAGAGTAATA AAGCACATCC TGACAATAAA GCAGAGTTTC CCAGTTATCT GCTGGGGGCA
    AGATCTGGTG CACTGAAACA TTTTGTCATT CCAAAAATCA AACGTGACAA AGATGGCAAT
    GTCACTCAGG AGTCAAGGAA AACTGAACTT AAAGGTGAAC AGAAGGACAA AGTAGAGAAG
    ATTGGGCTAG TTGAAGACCT AAATAAAGGA GCTAAGCCTG TAGTTGTCCT TCAGAAGCTT
    TCTTTGGACG ATGTACAAAA ATTTATTAAG GATAGAGAAG ATAAATCAAG GGGCTCTTGT
    TTTAAACCTA ACAAGAACAA GTCGTCCAAA TCTAATAAAG GTAGCATAGA TCAGTCAGTG
    CTAAAGGAGT TGCCTCCTGA GCTCCTGGCA GAAATTGAAT CCACCATGCC TCTTTGTGAA
    CGCGTGAAAA TGAACAAGCG CAAACGTAGC ACAGTTAATG AGAAACCAAA ATATGCTGAA
    ATCAGTTCTG ATGAAGACAA TGACAGTGAA GAAGCTTTTG AATCTTCTCG AAAAAGACAT
    AAAAAGGATA GAGATGAAGC TTGGGAGTAC GAAGAGCATG ACAGAAGAAG TTCTGGTGAC
    CACAGGAGGA GTGGTCATTA TCATGAAGGA AGAAGGACTT CTGGTAGTGG CCGTTACCGC
    AATCGCAGTC CCGATGACTC TGATCTGGAT GATTATTCTC CTCCTCCTAG CCTCAGTGAG
    GTGGCAAGAA AAATGAAGAA AAAAGAAAAG CAAAAGAAGA GGAGAGCTTA CGAGCCTAAA
    CTAACACCTG AAGAAATGAT GGATTCTTCA ACCTTCAAAA GATTTATTGC TTCAATAGAG
    AATATTCTGG AAAATCTGGA AGACATGGAT TTTACAACCT TGGGTGATGA TGATGAGATT
    CCACAGGAAT TGCTTTTGGG AAAACATCAG CTTAATGAGT TGGGTAGTGA ATCTGCAAAA
    ATCAAGGCAA TGGGTATTAT GGACAAGCTC TCAACAGATA AAATAGTAAA GGTCCTGAAC
    ATTTTGGAGA AGAATATTCA AGACGGATCA AAGCTTTCTA CATTACTTAA CCACAACAAT
    GACACTGAAG ATGAGGAGAG ATTATGGAGA GATCTCATAA TGGAGAGAGT AACAAAATCT
    GCAGATGCTT GTCTTACTGC TATCAATATT ATGACATCAC CAAATATGCC TAAAGCTGTT
    TATATTGAGG ACGTAATAGA AAGAGTTATT CAGTACACAA AATTTCATTT GCAGAACACT
    CTCTATCCTC AGTATGACCC TGTGTATAGA GTGGATCCTC TTGGTGGTGG TCTTTTAAGT
    TCAAAAGCAA AACGTGCCAA GTGTTCCACC CACAAACAAA GAGTTATAGT GATGTTGTAT
    AACAAAGTTT GTGATATTGT CAGCAGTTTG TCAGAGTTGC TAGAGATACA ACTTCTTACT
    GATACAACTA TTCTTCAGGT ATCGTCTATG GGGATAACAC CTTTCTTTGT AGAAAATGTC
    AGTGAACTAC AGCTATGTGC CATCAAATTA GTGACTGCAG TCTTCTCCAG GTATGAAAAA
    CATAGACAGC TAATACTAGA AGAAATTTTT ACTTCCCTTG CAAGACTACC AACTAGCAAG
    AGGAGCCTGA GAAACTTCAG GTTGAATAGC AGTGACACAG ATGGAGAGCC TATGTATATT
    CAGATGGTAA CAGCACTAGT TCTGCAGCTT ATTCAATGTG TCGTGCACTT ACCATCAGCA
    GAAAAAGATT CTAATTCAGA TGAGGAATTG AACAAAAAAG TGGATCAAGA TGTGCTTATT
    ACTAACTCAT ATGAAACAGC TATGAGAACA GCACAAAATT TTCTTTCTAT TTTTCTTAAG
    AAATGTGGTA GTAAACAAGG AGAAGAAGAT TACAGGCCTC TCTTTGAGAA CTTCATTCAA
    GATCTTCTTT CCACAGTCAA TAAACCAGAA TGGCCAGCTG CAGAGTTGCT GCTTAGCTTA
    CTAGGAAGGT TATTGGTTCA CCAGTTCAGT AATAAATCAA CAGAAATGGC TTTAAGAGTT
    GCGTCACTTG ACTATCTTGG AACCGTAGCT GCAAGACTGA GGAAAGATGC AGTCACTAGT
    AAAATGGATC AAGGATCTAT AGCTAGAATC CTCAAACAGG CCTCAGGAGG AGAAGATGAT
    ATTCAACAGC TACAGAAGGC TTTATTGGAT TATCTGGATG AGAACACTGA AACTGATGCA
    TCGTTAGTGT TTTCTCGGAA GTTTTATATA GCTCAGTGGT TTCGGGATAC AACTATGGAG
    ACAGAGAAGG CAATTAAATC TCAGAAGGAT GAGGATTCAT CTGAAGGAAC TCATCATGCA
    AAAGATGTTG AGACCACAGG ACAAATCATG CACAGAGCAG AAAGCCGCAA GAAGTTTCTC
    CGCAGCATCA TTAAAACTGC TCCATCTCAG TTCAGTACAT TAAAGATTTA TTCTGACACT
    GTGGACTATG AAGATGCATG TTTGATTGTT CGCTACTTGG CCTCTATGAG GCCGTTTGCC
    CAGAGCTTCG ATATTTATTT AACACAGATT CTGCGTGTAC TTGGTGAAAA TGCAATTGCA
    GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGATCC CAGTATTCTA
    GCCAGGCCGG ATATGCAGCG AGGTGTTCAT GGACGGTTAA TGGATAACTC CACCAGTGTA
    CGGGAAGCAG CTGTGGAGCT GCTTGGCCGA TTTGTGCTCT GTCGTCCTCA GCTTGCTGAG
    CAGTATTATG ACATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGAAAAAGA
    GTCATAAAGA TACTTAGGGA TATCTGCATT GAACAACCAA CGTTTCCCAA AATTACAGAG
    ATGTGTGTGA AAATGATTCG GAGAGTCAAT GATGAAGAAG GCATTAAGAA ATTAGTAAAT
    GAGACATTCC AGAAGCTGTG GTTTACTCCA ACTCCACACC ATGACAAAGA AGCAATGACT
    AGGAAAATAC TGAACATCAC TGATGTGGTG GCAGCTTGTA GGGATACAGG GTATGATTGG
    TTTGAGCAGC TTCTTCAAAA TCTATTGAAG TCAGAAGAGG ATGCTTCATA TAAACCTGTG
    AAGAAGGCTT GTACTCAACT TGTTGACAAT TTGGTTGAGC ACATCCTTAA ATATGAGGAG
    TCTTTATCAG ACTCTGACAA CAAAGGAGTG AATTCTGGTC GCTTGGTTGC TTGTATAACC
    ACTCTGTTCT TATTCAGCAA AATCAGGCCC CAGTTAATGG TCAAACATGC CATGACCATG
    CAGCCATATC TGGCAACTAA ATGTAGTACT CAGAATGATT TCATGGTGAT CTGCAATGTT
    GCAAAAATCT TGGAGCTTGT AGTGCCGTTA ATGGAACACC CAAGTGAGAC CTTCCTTGCC
    ACTATTGAGG AAGACCTCAT GAAACTCATC ATCAAATATG GCATGACAGT TGTTCAACAT
    TGTGTGAGCT GCCTTGGGTC TGTTGTGAAC AAGGTCACCC AGAATTACAA GTTTGTGTGG
    GCCTGTTTTA ATAGATACTA TGGTGCACTT TCAAAATTAA AAAGTCAACA CCAAGAAGAC
    CCCAACAGTA CAATACTTAC TGCCAATAAA CCAGCACTCC TTAGATCACT TTTCACTGTT
    GGAGCACTGT GTCGGCATTT TGATTTTGAC CATGAAGATT TTAAGGGCAA CAGTAAGGTT
    AACATTAAGG ATAAAGTACT TGAACTGCTG ATGTATTTTA CAAAGCATTC AGACGAAGAA
    GTACAGACAA AAGCCATTAT TGGCCTGGGA TTTGCATTTA TACAACACCC AAGCTTAATG
    TTTGAACAGG AAGTAAAGAC TCTGTACAAC AGCATTCTTT CAGATAAGAA CTGTTCAGTA
    AACCTAAAAA TCCAGGTGTT AAAAAACCTC CAGACCTACT TGCAGGAGGA GGACACACGT
    ATGCAGCAGG CAGACAGAGA CTGGAAAAAA GTAGCAAAAC AGGAAGACCT GAAAGAAATG
    GGTGATATTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TGTATCTCAA ACAGGTCCTT
    GAGGCTTTCT TTCATACCCA GTCCAATGTA CGCCACTTTG CTCTAAATGT CATTGCACTG
    ACGTTAAACC AGGGTCTCAT CCATCCTGTT CAGTGTGTGC CATACTTAAT TGCTATGGGC
    ACAGATCCAG AGCCTTCTAT GCGAAACAAA GCTGACCAGC AGTTGGTGGA AATAGACAAA
    AAGTATGCTG GTTTCATTCA CATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
    CAACAGGCAA TCAATACCTG CCCAAAGGAT CCTGTAAGAG GCTTTAGACA CGATGAATCA
    TCCAGTGCAT TGTGTTCACA CCTATATTCT ATGATCCGAG GGAACCGTCA ACACAGACGA
    GCATTTCTAA TTTCTTTACT CAATCTCTTC GATGATACTG CAAAAACAGA GGTGAATATG
    CTCTTGTACA TAGCAGACAA TCTAGCCTGT TTTCCTTATC AAACGCAGGA AGAGCCACTG
    TTTATAATGC ATCATATAGA CATTACACTA TCAGTTTCTG GTAGCAACCT ACTACAGTCA
    TTTAAAGAGT CAATGGTGAA AGACAAAAAA AAGGAAAGAA AGCCTTCATC AGATGAGGAG
    ACTGAGAGTG ACAGTAATAG TGGCAGTGAG AGCGAAAGTG AAGAGGAAGC TTCTAAGCCT
    CGGAGGTTAC GAAAACGAGT AGATTCTGAC TCAGATTCTG ATGAAGAAAA TGATATAAAT
    GCTGTGATGA AATGTTTACC AGAAAACTCA GCTCCTTTAA TTGAATTTGC AAATGTGTCT
    CAAGGCATAT TATTACTTTT GATGCTGAAA CAGCATTTAA AGAACCTTTG CGGATTCTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCT GAGTCTGCAA AAGTTTATGA TAAAGCGATA
    AACAGGAAGA CAGGAGTGCA TTTCCATCCA AAACAAACTC TGGATTTTCT ACGGAGTGAT
    ATGGCTAATT CCAAGATCAC TGAAGAAGTG AAGAGGAGTA TAGTAAAACA GTACTTAGAT
    TTCAAGCTCC TTATGGAACA TTTGGATCCT GATGAAGAAG AAGAAGATGG AGAGGTGTCA
    GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AACAATGCAG CTGAGACAGA GGACGATGAA AGTGATGGGG AGGATAGAGG AGGAGGCACT
    TCAGGGTCAT TGAGAAGGTC AAAACGAAAT TCAGACTCTA CGGAGTTGGC TTCACAGATG
    AATGAAAGTG TTGATGTCAT GGATGTCATC GCTATTTGCT GTCCAAAGTA CAAAGATCGA
    CCACAAATTG CAAGAGTAGT ACAGAAAACC AGCAATGGCT TCAGTGTTCA GTGGATGGCA
    GGCTCCTACA GTGGCTCCTG GACTGAGGCT AAGCGCCGTG ATGGCCGCAA ACTGGTGCCT
    TGGGTAGACA CTATTAAAGA GTCAGACATT ATTTACAAAA AAATTGCTCT AACGAGTGCT
    AATAAGCTGA CTAATAAAGT TGTTCAGACT TTACGATCGC TGTATGCCGC CAAGGATGGA
    ACTTCGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa20946
    Clone ID Related Accession (Same CDS sequence) XM_425012.5 , XM_425012.6 , XM_015277519.2 , XM_015277519.1 , XM_015277520.1 , XM_015277520.2
    Accession Version XM_015277520.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8352bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product nipped-B-like protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277520.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGATAAGCCT
    CTGAAGAAGC GAAAACAAGA AAACAAACCA CAGGAGGCTG GGGGTGCTGC TGGAGGAAAT
    AGGCCAGCTT CTCAGGAGTC AGGTTCTGCA GGAAATGGGG CAAGGCCAGC ACTGATGGTT
    AGTATTGATC TTCAACAAGC AGGAAGAGCG GACTCTCAGG CAACAATAAC TCAGGAATTT
    GAGGCTATCA AAAAACCTGA AGAGATTAAG CAGTATAATG ATGGATTTGT GTGTATTCCC
    CAAGAAGAGA CTGTTGGAGT TCTTAAACTT AAACCTGAAA ACCACCCTGA GATTTTTAGG
    AAAAAGTCAG ACTTTGAGGT TCAAAAGACA GATATCCAGC AAAATGAAAA CAGACAAATG
    GAGTTTCAGC AAAATGAGAG CAGAAGGTTG GAAAGTAAAT ACAATGACAA TAAACAGGTA
    GAAGCTAAAT ATGACATCAA GCATGACAGT AGACAAATGG AGATGAAACA AAATGAGAAC
    AGACAGGCAG AGTCAAAACA AAATGAGAGC AGACAAATGG AGATGAAACA AAATGAGGGA
    AAACAAAATA ATGGCAAACA AGAAATAAGA CAGAGACCTG AAACACCAAA GCAGAAGAGC
    GAAGGCAGGC CTGAAACACC CAAGCAGAAG AGTGAAGGCA GGCCTGAAAC ACCAAAACAT
    AGGCATGATA ACAGGAGGGA CTCCAGTAAA CCATTGTCAG AAAAGAAAAT TGAATTATCT
    AGGCATAAAC CTGATGTGAA ATCTGATACT TCAAAGGTAA AATCTGATAG TAGATCAGAT
    CCCGTGAAGC AAAGGCCTGA TGGGCGTTCA GTTTCTGATA CTCAGAGGCG TGACCACGAT
    AGCCTTAAAC AGAGATCTGA GGACAGGGAA TCAGAAAGAT ACAAAGGAGA TCCATCCAAG
    ATTAGAAGGA CTGAATTTTT GAGATCCTCA AACAGAAATG AACATGATTC TAAGTATGGT
    ATTAAATCTG ATGGTTCAAA ACCTGAAAAA TTTGAAAGGA AACACAGACA TGACTCTGGT
    GAATCAAGGG AAAGATTTTC TTCTGGGGAT CAGAAGTCAG ATCAAAAGTC AAGACCTGAC
    AGCCCTCGAG TTAAACAGGA TGGCAAAGGA GATTCTAGCA AATCAAGACC GGATAAACCT
    GGTTTTAGAT CACCCAACAG CAAGGATGAA CGAAGGACAG ACAGTAGTAA GAGTAAAGTA
    GAGAGTAATA AAGCACATCC TGACAATAAA GCAGAGTTTC CCAGTTATCT GCTGGGGGCA
    AGATCTGGTG CACTGAAACA TTTTGTCATT CCAAAAATCA AACGTGACAA AGATGGCAAT
    GTCACTCAGG AGTCAAGGAA AACTGAACTT AAAGGTGAAC AGAAGGACAA AGTAGAGAAG
    ATTGGGCTAG TTGAAGACCT AAATAAAGGA GCTAAGCCTG TAGTTGTCCT TCAGAAGCTT
    TCTTTGGACG ATGTACAAAA ATTTATTAAG GATAGAGAAG ATAAATCAAG GGGCTCTTGT
    TTTAAACCTA ACAAGAACAA GTCGTCCAAA TCTAATAAAG GTAGCATAGA TCAGTCAGTG
    CTAAAGGAGT TGCCTCCTGA GCTCCTGGCA GAAATTGAAT CCACCATGCC TCTTTGTGAA
    CGCGTGAAAA TGAACAAGCG CAAACGTAGC ACAGTTAATG AGAAACCAAA ATATGCTGAA
    ATCAGTTCTG ATGAAGACAA TGACAGTGAA GAAGCTTTTG AATCTTCTCG AAAAAGACAT
    AAAAAGGATA GAGATGAAGC TTGGGAGTAC GAAGAGCATG ACAGAAGAAG TTCTGGTGAC
    CACAGGAGGA GTGGTCATTA TCATGAAGGA AGAAGGACTT CTGGTAGTGG CCGTTACCGC
    AATCGCAGTC CCGATGACTC TGATCTGGAT GATTATTCTC CTCCTCCTAG CCTCAGTGAG
    GTGGCAAGAA AAATGAAGAA AAAAGAAAAG CAAAAGAAGA GGAGAGCTTA CGAGCCTAAA
    CTAACACCTG AAGAAATGAT GGATTCTTCA ACCTTCAAAA GATTTATTGC TTCAATAGAG
    AATATTCTGG AAAATCTGGA AGACATGGAT TTTACAACCT TGGGTGATGA TGATGAGATT
    CCACAGGAAT TGCTTTTGGG AAAACATCAG CTTAATGAGT TGGGTAGTGA ATCTGCAAAA
    ATCAAGGCAA TGGGTATTAT GGACAAGCTC TCAACAGATA AAATAGTAAA GGTCCTGAAC
    ATTTTGGAGA AGAATATTCA AGACGGATCA AAGCTTTCTA CATTACTTAA CCACAACAAT
    GACACTGAAG ATGAGGAGAG ATTATGGAGA GATCTCATAA TGGAGAGAGT AACAAAATCT
    GCAGATGCTT GTCTTACTGC TATCAATATT ATGACATCAC CAAATATGCC TAAAGCTGTT
    TATATTGAGG ACGTAATAGA AAGAGTTATT CAGTACACAA AATTTCATTT GCAGAACACT
    CTCTATCCTC AGTATGACCC TGTGTATAGA GTGGATCCTC TTGGTGGTGG TCTTTTAAGT
    TCAAAAGCAA AACGTGCCAA GTGTTCCACC CACAAACAAA GAGTTATAGT GATGTTGTAT
    AACAAAGTTT GTGATATTGT CAGCAGTTTG TCAGAGTTGC TAGAGATACA ACTTCTTACT
    GATACAACTA TTCTTCAGGT ATCGTCTATG GGGATAACAC CTTTCTTTGT AGAAAATGTC
    AGTGAACTAC AGCTATGTGC CATCAAATTA GTGACTGCAG TCTTCTCCAG GTATGAAAAA
    CATAGACAGC TAATACTAGA AGAAATTTTT ACTTCCCTTG CAAGACTACC AACTAGCAAG
    AGGAGCCTGA GAAACTTCAG GTTGAATAGC AGTGACACAG ATGGAGAGCC TATGTATATT
    CAGATGGTAA CAGCACTAGT TCTGCAGCTT ATTCAATGTG TCGTGCACTT ACCATCAGCA
    GAAAAAGATT CTAATTCAGA TGAGGAATTG AACAAAAAAG TGGATCAAGA TGTGCTTATT
    ACTAACTCAT ATGAAACAGC TATGAGAACA GCACAAAATT TTCTTTCTAT TTTTCTTAAG
    AAATGTGGTA GTAAACAAGG AGAAGAAGAT TACAGGCCTC TCTTTGAGAA CTTCATTCAA
    GATCTTCTTT CCACAGTCAA TAAACCAGAA TGGCCAGCTG CAGAGTTGCT GCTTAGCTTA
    CTAGGAAGGT TATTGGTTCA CCAGTTCAGT AATAAATCAA CAGAAATGGC TTTAAGAGTT
    GCGTCACTTG ACTATCTTGG AACCGTAGCT GCAAGACTGA GGAAAGATGC AGTCACTAGT
    AAAATGGATC AAGGATCTAT AGCTAGAATC CTCAAACAGG CCTCAGGAGG AGAAGATGAT
    ATTCAACAGC TACAGAAGGC TTTATTGGAT TATCTGGATG AGAACACTGA AACTGATGCA
    TCGTTAGTGT TTTCTCGGAA GTTTTATATA GCTCAGTGGT TTCGGGATAC AACTATGGAG
    ACAGAGAAGG CAATTAAATC TCAGAAGGAT GAGGATTCAT CTGAAGGAAC TCATCATGCA
    AAAGATGTTG AGACCACAGG ACAAATCATG CACAGAGCAG AAAGCCGCAA GAAGTTTCTC
    CGCAGCATCA TTAAAACTGC TCCATCTCAG TTCAGTACAT TAAAGATTTA TTCTGACACT
    GTGGACTATG AAGATGCATG TTTGATTGTT CGCTACTTGG CCTCTATGAG GCCGTTTGCC
    CAGAGCTTCG ATATTTATTT AACACAGATT CTGCGTGTAC TTGGTGAAAA TGCAATTGCA
    GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGATCC CAGTATTCTA
    GCCAGGCCGG ATATGCAGCG AGGTGTTCAT GGACGGTTAA TGGATAACTC CACCAGTGTA
    CGGGAAGCAG CTGTGGAGCT GCTTGGCCGA TTTGTGCTCT GTCGTCCTCA GCTTGCTGAG
    CAGTATTATG ACATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGAAAAAGA
    GTCATAAAGA TACTTAGGGA TATCTGCATT GAACAACCAA CGTTTCCCAA AATTACAGAG
    ATGTGTGTGA AAATGATTCG GAGAGTCAAT GATGAAGAAG GCATTAAGAA ATTAGTAAAT
    GAGACATTCC AGAAGCTGTG GTTTACTCCA ACTCCACACC ATGACAAAGA AGCAATGACT
    AGGAAAATAC TGAACATCAC TGATGTGGTG GCAGCTTGTA GGGATACAGG GTATGATTGG
    TTTGAGCAGC TTCTTCAAAA TCTATTGAAG TCAGAAGAGG ATGCTTCATA TAAACCTGTG
    AAGAAGGCTT GTACTCAACT TGTTGACAAT TTGGTTGAGC ACATCCTTAA ATATGAGGAG
    TCTTTATCAG ACTCTGACAA CAAAGGAGTG AATTCTGGTC GCTTGGTTGC TTGTATAACC
    ACTCTGTTCT TATTCAGCAA AATCAGGCCC CAGTTAATGG TCAAACATGC CATGACCATG
    CAGCCATATC TGGCAACTAA ATGTAGTACT CAGAATGATT TCATGGTGAT CTGCAATGTT
    GCAAAAATCT TGGAGCTTGT AGTGCCGTTA ATGGAACACC CAAGTGAGAC CTTCCTTGCC
    ACTATTGAGG AAGACCTCAT GAAACTCATC ATCAAATATG GCATGACAGT TGTTCAACAT
    TGTGTGAGCT GCCTTGGGTC TGTTGTGAAC AAGGTCACCC AGAATTACAA GTTTGTGTGG
    GCCTGTTTTA ATAGATACTA TGGTGCACTT TCAAAATTAA AAAGTCAACA CCAAGAAGAC
    CCCAACAGTA CAATACTTAC TGCCAATAAA CCAGCACTCC TTAGATCACT TTTCACTGTT
    GGAGCACTGT GTCGGCATTT TGATTTTGAC CATGAAGATT TTAAGGGCAA CAGTAAGGTT
    AACATTAAGG ATAAAGTACT TGAACTGCTG ATGTATTTTA CAAAGCATTC AGACGAAGAA
    GTACAGACAA AAGCCATTAT TGGCCTGGGA TTTGCATTTA TACAACACCC AAGCTTAATG
    TTTGAACAGG AAGTAAAGAC TCTGTACAAC AGCATTCTTT CAGATAAGAA CTGTTCAGTA
    AACCTAAAAA TCCAGGTGTT AAAAAACCTC CAGACCTACT TGCAGGAGGA GGACACACGT
    ATGCAGCAGG CAGACAGAGA CTGGAAAAAA GTAGCAAAAC AGGAAGACCT GAAAGAAATG
    GGTGATATTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TGTATCTCAA ACAGGTCCTT
    GAGGCTTTCT TTCATACCCA GTCCAATGTA CGCCACTTTG CTCTAAATGT CATTGCACTG
    ACGTTAAACC AGGGTCTCAT CCATCCTGTT CAGTGTGTGC CATACTTAAT TGCTATGGGC
    ACAGATCCAG AGCCTTCTAT GCGAAACAAA GCTGACCAGC AGTTGGTGGA AATAGACAAA
    AAGTATGCTG GTTTCATTCA CATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
    CAACAGGCAA TCAATACCTG CCCAAAGGAT CCTGTAAGAG GCTTTAGACA CGATGAATCA
    TCCAGTGCAT TGTGTTCACA CCTATATTCT ATGATCCGAG GGAACCGTCA ACACAGACGA
    GCATTTCTAA TTTCTTTACT CAATCTCTTC GATGATACTG CAAAAACAGA GGTGAATATG
    CTCTTGTACA TAGCAGACAA TCTAGCCTGT TTTCCTTATC AAACGCAGGA AGAGCCACTG
    TTTATAATGC ATCATATAGA CATTACACTA TCAGTTTCTG GTAGCAACCT ACTACAGTCA
    TTTAAAGAGT CAATGGTGAA AGACAAAAAA AAGGAAAGAA AGCCTTCATC AGATGAGGAG
    ACTGAGAGTG ACAGTAATAG TGGCAGTGAG AGCGAAAGTG AAGAGGAAGC TTCTAAGCCT
    CGGAGGTTAC GAAAACGAGT AGATTCTGAC TCAGATTCTG ATGAAGAAAA TGATATAAAT
    GCTGTGATGA AATGTTTACC AGAAAACTCA GCTCCTTTAA TTGAATTTGC AAATGTGTCT
    CAAGGCATAT TATTACTTTT GATGCTGAAA CAGCATTTAA AGAACCTTTG CGGATTCTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCT GAGTCTGCAA AAGTTTATGA TAAAGCGATA
    AACAGGAAGA CAGGAGTGCA TTTCCATCCA AAACAAACTC TGGATTTTCT ACGGAGTGAT
    ATGGCTAATT CCAAGATCAC TGAAGAAGTG AAGAGGAGTA TAGTAAAACA GTACTTAGAT
    TTCAAGCTCC TTATGGAACA TTTGGATCCT GATGAAGAAG AAGAAGATGG AGAGGTGTCA
    GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AACAATGCAG CTGAGACAGA GGACGATGAA AGTGATGGGG AGGATAGAGG AGGAGGCACT
    TCAGGGTCAT TGAGAAGGTC AAAACGAAAT TCAGACTCTA CGGAGTTGGC TTCACAGATG
    AATGAAAGTG TTGATGTCAT GGATGTCATC GCTATTTGCT GTCCAAAGTA CAAAGATCGA
    CCACAAATTG CAAGAGTAGT ACAGAAAACC AGCAATGGCT TCAGTGTTCA GTGGATGGCA
    GGCTCCTACA GTGGCTCCTG GACTGAGGCT AAGCGCCGTG ATGGCCGCAA ACTGGTGCCT
    TGGGTAGACA CTATTAAAGA GTCAGACATT ATTTACAAAA AAATTGCTCT AACGAGTGCT
    AATAAGCTGA CTAATAAAGT TGTTCAGACT TTACGATCGC TGTATGCCGC CAAGGATGGA
    ACTTCGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133006.1
    CDS397..8748
    Translation

    Target ORF information:

    RefSeq Version XM_015277520.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277520.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGATAAGCCT
    CTGAAGAAGC GAAAACAAGA AAACAAACCA CAGGAGGCTG GGGGTGCTGC TGGAGGAAAT
    AGGCCAGCTT CTCAGGAGTC AGGTTCTGCA GGAAATGGGG CAAGGCCAGC ACTGATGGTT
    AGTATTGATC TTCAACAAGC AGGAAGAGCG GACTCTCAGG CAACAATAAC TCAGGAATTT
    GAGGCTATCA AAAAACCTGA AGAGATTAAG CAGTATAATG ATGGATTTGT GTGTATTCCC
    CAAGAAGAGA CTGTTGGAGT TCTTAAACTT AAACCTGAAA ACCACCCTGA GATTTTTAGG
    AAAAAGTCAG ACTTTGAGGT TCAAAAGACA GATATCCAGC AAAATGAAAA CAGACAAATG
    GAGTTTCAGC AAAATGAGAG CAGAAGGTTG GAAAGTAAAT ACAATGACAA TAAACAGGTA
    GAAGCTAAAT ATGACATCAA GCATGACAGT AGACAAATGG AGATGAAACA AAATGAGAAC
    AGACAGGCAG AGTCAAAACA AAATGAGAGC AGACAAATGG AGATGAAACA AAATGAGGGA
    AAACAAAATA ATGGCAAACA AGAAATAAGA CAGAGACCTG AAACACCAAA GCAGAAGAGC
    GAAGGCAGGC CTGAAACACC CAAGCAGAAG AGTGAAGGCA GGCCTGAAAC ACCAAAACAT
    AGGCATGATA ACAGGAGGGA CTCCAGTAAA CCATTGTCAG AAAAGAAAAT TGAATTATCT
    AGGCATAAAC CTGATGTGAA ATCTGATACT TCAAAGGTAA AATCTGATAG TAGATCAGAT
    CCCGTGAAGC AAAGGCCTGA TGGGCGTTCA GTTTCTGATA CTCAGAGGCG TGACCACGAT
    AGCCTTAAAC AGAGATCTGA GGACAGGGAA TCAGAAAGAT ACAAAGGAGA TCCATCCAAG
    ATTAGAAGGA CTGAATTTTT GAGATCCTCA AACAGAAATG AACATGATTC TAAGTATGGT
    ATTAAATCTG ATGGTTCAAA ACCTGAAAAA TTTGAAAGGA AACACAGACA TGACTCTGGT
    GAATCAAGGG AAAGATTTTC TTCTGGGGAT CAGAAGTCAG ATCAAAAGTC AAGACCTGAC
    AGCCCTCGAG TTAAACAGGA TGGCAAAGGA GATTCTAGCA AATCAAGACC GGATAAACCT
    GGTTTTAGAT CACCCAACAG CAAGGATGAA CGAAGGACAG ACAGTAGTAA GAGTAAAGTA
    GAGAGTAATA AAGCACATCC TGACAATAAA GCAGAGTTTC CCAGTTATCT GCTGGGGGCA
    AGATCTGGTG CACTGAAACA TTTTGTCATT CCAAAAATCA AACGTGACAA AGATGGCAAT
    GTCACTCAGG AGTCAAGGAA AACTGAACTT AAAGGTGAAC AGAAGGACAA AGTAGAGAAG
    ATTGGGCTAG TTGAAGACCT AAATAAAGGA GCTAAGCCTG TAGTTGTCCT TCAGAAGCTT
    TCTTTGGACG ATGTACAAAA ATTTATTAAG GATAGAGAAG ATAAATCAAG GGGCTCTTGT
    TTTAAACCTA ACAAGAACAA GTCGTCCAAA TCTAATAAAG GTAGCATAGA TCAGTCAGTG
    CTAAAGGAGT TGCCTCCTGA GCTCCTGGCA GAAATTGAAT CCACCATGCC TCTTTGTGAA
    CGCGTGAAAA TGAACAAGCG CAAACGTAGC ACAGTTAATG AGAAACCAAA ATATGCTGAA
    ATCAGTTCTG ATGAAGACAA TGACAGTGAA GAAGCTTTTG AATCTTCTCG AAAAAGACAT
    AAAAAGGATA GAGATGAAGC TTGGGAGTAC GAAGAGCATG ACAGAAGAAG TTCTGGTGAC
    CACAGGAGGA GTGGTCATTA TCATGAAGGA AGAAGGACTT CTGGTAGTGG CCGTTACCGC
    AATCGCAGTC CCGATGACTC TGATCTGGAT GATTATTCTC CTCCTCCTAG CCTCAGTGAG
    GTGGCAAGAA AAATGAAGAA AAAAGAAAAG CAAAAGAAGA GGAGAGCTTA CGAGCCTAAA
    CTAACACCTG AAGAAATGAT GGATTCTTCA ACCTTCAAAA GATTTATTGC TTCAATAGAG
    AATATTCTGG AAAATCTGGA AGACATGGAT TTTACAACCT TGGGTGATGA TGATGAGATT
    CCACAGGAAT TGCTTTTGGG AAAACATCAG CTTAATGAGT TGGGTAGTGA ATCTGCAAAA
    ATCAAGGCAA TGGGTATTAT GGACAAGCTC TCAACAGATA AAATAGTAAA GGTCCTGAAC
    ATTTTGGAGA AGAATATTCA AGACGGATCA AAGCTTTCTA CATTACTTAA CCACAACAAT
    GACACTGAAG ATGAGGAGAG ATTATGGAGA GATCTCATAA TGGAGAGAGT AACAAAATCT
    GCAGATGCTT GTCTTACTGC TATCAATATT ATGACATCAC CAAATATGCC TAAAGCTGTT
    TATATTGAGG ACGTAATAGA AAGAGTTATT CAGTACACAA AATTTCATTT GCAGAACACT
    CTCTATCCTC AGTATGACCC TGTGTATAGA GTGGATCCTC TTGGTGGTGG TCTTTTAAGT
    TCAAAAGCAA AACGTGCCAA GTGTTCCACC CACAAACAAA GAGTTATAGT GATGTTGTAT
    AACAAAGTTT GTGATATTGT CAGCAGTTTG TCAGAGTTGC TAGAGATACA ACTTCTTACT
    GATACAACTA TTCTTCAGGT ATCGTCTATG GGGATAACAC CTTTCTTTGT AGAAAATGTC
    AGTGAACTAC AGCTATGTGC CATCAAATTA GTGACTGCAG TCTTCTCCAG GTATGAAAAA
    CATAGACAGC TAATACTAGA AGAAATTTTT ACTTCCCTTG CAAGACTACC AACTAGCAAG
    AGGAGCCTGA GAAACTTCAG GTTGAATAGC AGTGACACAG ATGGAGAGCC TATGTATATT
    CAGATGGTAA CAGCACTAGT TCTGCAGCTT ATTCAATGTG TCGTGCACTT ACCATCAGCA
    GAAAAAGATT CTAATTCAGA TGAGGAATTG AACAAAAAAG TGGATCAAGA TGTGCTTATT
    ACTAACTCAT ATGAAACAGC TATGAGAACA GCACAAAATT TTCTTTCTAT TTTTCTTAAG
    AAATGTGGTA GTAAACAAGG AGAAGAAGAT TACAGGCCTC TCTTTGAGAA CTTCATTCAA
    GATCTTCTTT CCACAGTCAA TAAACCAGAA TGGCCAGCTG CAGAGTTGCT GCTTAGCTTA
    CTAGGAAGGT TATTGGTTCA CCAGTTCAGT AATAAATCAA CAGAAATGGC TTTAAGAGTT
    GCGTCACTTG ACTATCTTGG AACCGTAGCT GCAAGACTGA GGAAAGATGC AGTCACTAGT
    AAAATGGATC AAGGATCTAT AGCTAGAATC CTCAAACAGG CCTCAGGAGG AGAAGATGAT
    ATTCAACAGC TACAGAAGGC TTTATTGGAT TATCTGGATG AGAACACTGA AACTGATGCA
    TCGTTAGTGT TTTCTCGGAA GTTTTATATA GCTCAGTGGT TTCGGGATAC AACTATGGAG
    ACAGAGAAGG CAATTAAATC TCAGAAGGAT GAGGATTCAT CTGAAGGAAC TCATCATGCA
    AAAGATGTTG AGACCACAGG ACAAATCATG CACAGAGCAG AAAGCCGCAA GAAGTTTCTC
    CGCAGCATCA TTAAAACTGC TCCATCTCAG TTCAGTACAT TAAAGATTTA TTCTGACACT
    GTGGACTATG AAGATGCATG TTTGATTGTT CGCTACTTGG CCTCTATGAG GCCGTTTGCC
    CAGAGCTTCG ATATTTATTT AACACAGATT CTGCGTGTAC TTGGTGAAAA TGCAATTGCA
    GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGATCC CAGTATTCTA
    GCCAGGCCGG ATATGCAGCG AGGTGTTCAT GGACGGTTAA TGGATAACTC CACCAGTGTA
    CGGGAAGCAG CTGTGGAGCT GCTTGGCCGA TTTGTGCTCT GTCGTCCTCA GCTTGCTGAG
    CAGTATTATG ACATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGAAAAAGA
    GTCATAAAGA TACTTAGGGA TATCTGCATT GAACAACCAA CGTTTCCCAA AATTACAGAG
    ATGTGTGTGA AAATGATTCG GAGAGTCAAT GATGAAGAAG GCATTAAGAA ATTAGTAAAT
    GAGACATTCC AGAAGCTGTG GTTTACTCCA ACTCCACACC ATGACAAAGA AGCAATGACT
    AGGAAAATAC TGAACATCAC TGATGTGGTG GCAGCTTGTA GGGATACAGG GTATGATTGG
    TTTGAGCAGC TTCTTCAAAA TCTATTGAAG TCAGAAGAGG ATGCTTCATA TAAACCTGTG
    AAGAAGGCTT GTACTCAACT TGTTGACAAT TTGGTTGAGC ACATCCTTAA ATATGAGGAG
    TCTTTATCAG ACTCTGACAA CAAAGGAGTG AATTCTGGTC GCTTGGTTGC TTGTATAACC
    ACTCTGTTCT TATTCAGCAA AATCAGGCCC CAGTTAATGG TCAAACATGC CATGACCATG
    CAGCCATATC TGGCAACTAA ATGTAGTACT CAGAATGATT TCATGGTGAT CTGCAATGTT
    GCAAAAATCT TGGAGCTTGT AGTGCCGTTA ATGGAACACC CAAGTGAGAC CTTCCTTGCC
    ACTATTGAGG AAGACCTCAT GAAACTCATC ATCAAATATG GCATGACAGT TGTTCAACAT
    TGTGTGAGCT GCCTTGGGTC TGTTGTGAAC AAGGTCACCC AGAATTACAA GTTTGTGTGG
    GCCTGTTTTA ATAGATACTA TGGTGCACTT TCAAAATTAA AAAGTCAACA CCAAGAAGAC
    CCCAACAGTA CAATACTTAC TGCCAATAAA CCAGCACTCC TTAGATCACT TTTCACTGTT
    GGAGCACTGT GTCGGCATTT TGATTTTGAC CATGAAGATT TTAAGGGCAA CAGTAAGGTT
    AACATTAAGG ATAAAGTACT TGAACTGCTG ATGTATTTTA CAAAGCATTC AGACGAAGAA
    GTACAGACAA AAGCCATTAT TGGCCTGGGA TTTGCATTTA TACAACACCC AAGCTTAATG
    TTTGAACAGG AAGTAAAGAC TCTGTACAAC AGCATTCTTT CAGATAAGAA CTGTTCAGTA
    AACCTAAAAA TCCAGGTGTT AAAAAACCTC CAGACCTACT TGCAGGAGGA GGACACACGT
    ATGCAGCAGG CAGACAGAGA CTGGAAAAAA GTAGCAAAAC AGGAAGACCT GAAAGAAATG
    GGTGATATTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TGTATCTCAA ACAGGTCCTT
    GAGGCTTTCT TTCATACCCA GTCCAATGTA CGCCACTTTG CTCTAAATGT CATTGCACTG
    ACGTTAAACC AGGGTCTCAT CCATCCTGTT CAGTGTGTGC CATACTTAAT TGCTATGGGC
    ACAGATCCAG AGCCTTCTAT GCGAAACAAA GCTGACCAGC AGTTGGTGGA AATAGACAAA
    AAGTATGCTG GTTTCATTCA CATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
    CAACAGGCAA TCAATACCTG CCCAAAGGAT CCTGTAAGAG GCTTTAGACA CGATGAATCA
    TCCAGTGCAT TGTGTTCACA CCTATATTCT ATGATCCGAG GGAACCGTCA ACACAGACGA
    GCATTTCTAA TTTCTTTACT CAATCTCTTC GATGATACTG CAAAAACAGA GGTGAATATG
    CTCTTGTACA TAGCAGACAA TCTAGCCTGT TTTCCTTATC AAACGCAGGA AGAGCCACTG
    TTTATAATGC ATCATATAGA CATTACACTA TCAGTTTCTG GTAGCAACCT ACTACAGTCA
    TTTAAAGAGT CAATGGTGAA AGACAAAAAA AAGGAAAGAA AGCCTTCATC AGATGAGGAG
    ACTGAGAGTG ACAGTAATAG TGGCAGTGAG AGCGAAAGTG AAGAGGAAGC TTCTAAGCCT
    CGGAGGTTAC GAAAACGAGT AGATTCTGAC TCAGATTCTG ATGAAGAAAA TGATATAAAT
    GCTGTGATGA AATGTTTACC AGAAAACTCA GCTCCTTTAA TTGAATTTGC AAATGTGTCT
    CAAGGCATAT TATTACTTTT GATGCTGAAA CAGCATTTAA AGAACCTTTG CGGATTCTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCT GAGTCTGCAA AAGTTTATGA TAAAGCGATA
    AACAGGAAGA CAGGAGTGCA TTTCCATCCA AAACAAACTC TGGATTTTCT ACGGAGTGAT
    ATGGCTAATT CCAAGATCAC TGAAGAAGTG AAGAGGAGTA TAGTAAAACA GTACTTAGAT
    TTCAAGCTCC TTATGGAACA TTTGGATCCT GATGAAGAAG AAGAAGATGG AGAGGTGTCA
    GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AACAATGCAG CTGAGACAGA GGACGATGAA AGTGATGGGG AGGATAGAGG AGGAGGCACT
    TCAGGGTCAT TGAGAAGGTC AAAACGAAAT TCAGACTCTA CGGAGTTGGC TTCACAGATG
    AATGAAAGTG TTGATGTCAT GGATGTCATC GCTATTTGCT GTCCAAAGTA CAAAGATCGA
    CCACAAATTG CAAGAGTAGT ACAGAAAACC AGCAATGGCT TCAGTGTTCA GTGGATGGCA
    GGCTCCTACA GTGGCTCCTG GACTGAGGCT AAGCGCCGTG ATGGCCGCAA ACTGGTGCCT
    TGGGTAGACA CTATTAAAGA GTCAGACATT ATTTACAAAA AAATTGCTCT AACGAGTGCT
    AATAAGCTGA CTAATAAAGT TGTTCAGACT TTACGATCGC TGTATGCCGC CAAGGATGGA
    ACTTCGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa20947
    Clone ID Related Accession (Same CDS sequence) XM_004937193.3 , XM_004937193.2
    Accession Version XM_004937193.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product nipped-B-like protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004937193.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    3721
    3781
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4621
    4681
    4741
    4801
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    4921
    4981
    5041
    5101
    5161
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    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGATAAGCCT
    CTGAAGAAGC GAAAACAAGA AAACAAACCA CAGGAGGCTG GGGGTGCTGC TGGAGGAAAT
    AGGCCAGCTT CTCAGGAGTC AGGTTCTGCA GGAAATGGGG CAAGGCCAGC ACTGATGGTT
    AGTATTGATC TTCAACAAGC AGGAAGAGCG GACTCTCAGG CAACAATAAC TCAGGAATTT
    GAGGCTATCA AAAAACCTGA AGAGATTAAG CAGTATAATG ATGGATTTGT GTGTATTCCC
    CAAGAAGAGA CTGTTGGAGT TCTTAAACTT AAACCTGAAA ACCACCCTGA GATTTTTAGG
    AAAAAGTCAG ACTTTGAGGT TCAAAAGACA GATATCCAGC AAAATGAAAA CAGACAAATG
    GAGTTTCAGC AAAATGAGAG CAGAAGGTTG GAAAGTAAAT ACAATGACAA TAAACAGGTA
    GAAGCTAAAT ATGACATCAA GCATGACAGT AGACAAATGG AGATGAAACA AAATGAGAAC
    AGACAGGCAG AGTCAAAACA AAATGAGAGC AGACAAATGG AGATGAAACA AAATGAGGGA
    AAACAAAATA ATGGCAAACA AGAAATAAGA CAGAGACCTG AAACACCAAA GCAGAAGAGC
    GAAGGCAGGC CTGAAACACC CAAGCAGAAG AGTGAAGGCA GGCCTGAAAC ACCAAAACAT
    AGGCATGATA ACAGGAGGGA CTCCAGTAAA CCATTGTCAG AAAAGAAAAT TGAATTATCT
    AGGCATAAAC CTGATGTGAA ATCTGATACT TCAAAGGTAA AATCTGATAG TAGATCAGAT
    CCCGTGAAGC AAAGGCCTGA TGGGCGTTCA GTTTCTGATA CTCAGAGGCG TGACCACGAT
    AGCCTTAAAC AGAGATCTGA GGACAGGGAA TCAGAAAGAT ACAAAGGAGA TCCATCCAAG
    ATTAGAAGGA CTGAATTTTT GAGATCCTCA AACAGAAATG AACATGATTC TAAGTATGGT
    ATTAAATCTG ATGGTTCAAA ACCTGAAAAA TTTGAAAGGA AACACAGACA TGACTCTGGT
    GAATCAAGGG AAAGATTTTC TTCTGGGGAT CAGAAGTCAG ATCAAAAGTC AAGACCTGAC
    AGCCCTCGAG TTAAACAGGA TGGCAAAGGA GATTCTAGCA AATCAAGACC GGATAAACCT
    GGTTTTAGAT CACCCAACAG CAAGGATGAA CGAAGGACAG ACAGTAGTAA GAGTAAAGTA
    GAGAGTAATA AAGCACATCC TGACAATAAA GCAGAGTTTC CCAGTTATCT GCTGGGGGCA
    AGATCTGGTG CACTGAAACA TTTTGTCATT CCAAAAATCA AACGTGACAA AGATGGCAAT
    GTCACTCAGG AGTCAAGGAA AACTGAACTT AAAGGTGAAC AGAAGGACAA AGTAGAGAAG
    ATTGGGCTAG TTGAAGACCT AAATAAAGGA GCTAAGCCTG TAGTTGTCCT TCAGAAGCTT
    TCTTTGGACG ATGTACAAAA ATTTATTAAG GATAGAGAAG ATAAATCAAG GGGCTCTTGT
    TTTAAACCTA ACAAGAACAA GTCGTCCAAA TCTAATAAAG GTAGCATAGA TCAGTCAGTG
    CTAAAGGAGT TGCCTCCTGA GCTCCTGGCA GAAATTGAAT CCACCATGCC TCTTTGTGAA
    CGCGTGAAAA TGAACAAGCG CAAACGTAGC ACAGTTAATG AGAAACCAAA ATATGCTGAA
    ATCAGTTCTG ATGAAGACAA TGACAGTGAA GAAGCTTTTG AATCTTCTCG AAAAAGACAT
    AAAAAGGATA GAGATGAAGC TTGGGAGTAC GAAGAGCATG ACAGAAGAAG TTCTGGTGAC
    CACAGGAGGA GTGGTCATTA TCATGAAGGA AGAAGGACTT CTGGTAGTGG CCGTTACCGC
    AATCGCAGTC CCGATGACTC TGATCTGGAT GATTATTCTC CTCCTCCTAG CCTCAGTGAG
    GTGGCAAGAA AAATGAAGAA AAAAGAAAAG CAAAAGAAGA GGAGAGCTTA CGAGCCTAAA
    CTAACACCTG AAGAAATGAT GGATTCTTCA ACCTTCAAAA GATTTATTGC TTCAATAGAG
    AATATTCTGG AAAATCTGGA AGACATGGAT TTTACAACCT TGGGTGATGA TGATGAGATT
    CCACAGGAAT TGCTTTTGGG AAAACATCAG CTTAATGAGT TGGGTAGTGA ATCTGCAAAA
    ATCAAGGCAA TGGGTATTAT GGACAAGCTC TCAACAGATA AAATAGTAAA GGTCCTGAAC
    ATTTTGGAGA AGAATATTCA AGACGGATCA AAGCTTTCTA CATTACTTAA CCACAACAAT
    GACACTGAAG ATGAGGAGAG ATTATGGAGA GATCTCATAA TGGAGAGAGT AACAAAATCT
    GCAGATGCTT GTCTTACTGC TATCAATATT ATGACATCAC CAAATATGCC TAAAGCTGTT
    TATATTGAGG ACGTAATAGA AAGAGTTATT CAGTACACAA AATTTCATTT GCAGAACACT
    CTCTATCCTC AGTATGACCC TGTGTATAGA GTGGATCCTC TTGGTGGTGG TCTTTTAAGT
    TCAAAAGCAA AACGTGCCAA GTGTTCCACC CACAAACAAA GAGTTATAGT GATGTTGTAT
    AACAAAGTTT GTGATATTGT CAGCAGTTTG TCAGAGTTGC TAGAGATACA ACTTCTTACT
    GATACAACTA TTCTTCAGGT ATCGTCTATG GGGATAACAC CTTTCTTTGT AGAAAATGTC
    AGTGAACTAC AGCTATGTGC CATCAAATTA GTGACTGCAG TCTTCTCCAG GTATGAAAAA
    CATAGACAGC TAATACTAGA AGAAATTTTT ACTTCCCTTG CAAGACTACC AACTAGCAAG
    AGGAGCCTGA GAAACTTCAG GTTGAATAGC AGTGACACAG ATGGAGAGCC TATGTATATT
    CAGATGGTAA CAGCACTAGT TCTGCAGCTT ATTCAATGTG TCGTGCACTT ACCATCAGCA
    GAAAAAGATT CTAATTCAGA TGAGGAATTG AACAAAAAAG TGGATCAAGA TGTGCTTATT
    ACTAACTCAT ATGAAACAGC TATGAGAACA GCACAAAATT TTCTTTCTAT TTTTCTTAAG
    AAATGTGGTA GTAAACAAGG AGAAGAAGAT TACAGGCCTC TCTTTGAGAA CTTCATTCAA
    GATCTTCTTT CCACAGTCAA TAAACCAGAA TGGCCAGCTG CAGAGTTGCT GCTTAGCTTA
    CTAGGAAGGT TATTGGTTCA CCAGTTCAGT AATAAATCAA CAGAAATGGC TTTAAGAGTT
    GCGTCACTTG ACTATCTTGG AACCGTAGCT GCAAGACTGA GGAAAGATGC AGTCACTAGT
    AAAATGGATC AAGGATCTAT AGCTAGAATC CTCAAACAGG CCTCAGGAGG AGAAGATGAT
    ATTCAACAGC TACAGAAGGC TTTATTGGAT TATCTGGATG AGAACACTGA AACTGATGCA
    TCGTTAGTGT TTTCTCGGAA GTTTTATATA GCTCAGTGGT TTCGGGATAC AACTATGGAG
    ACAGAGAAGG CAATTAAATC TCAGAAGGAT GAGGATTCAT CTGAAGGAAC TCATCATGCA
    AAAGATGTTG AGACCACAGG ACAAATCATG CACAGAGCAG AAAGCCGCAA GAAGTTTCTC
    CGCAGCATCA TTAAAACTGC TCCATCTCAG TTCAGTACAT TAAAGATTTA TTCTGACACT
    GTGGACTATG AAGATGCATG TTTGATTGTT CGCTACTTGG CCTCTATGAG GCCGTTTGCC
    CAGAGCTTCG ATATTTATTT AACACAGATT CTGCGTGTAC TTGGTGAAAA TGCAATTGCA
    GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGATCC CAGTATTCTA
    GCCAGGCCGG ATATGCAGCG AGGTGTTCAT GGACGGTTAA TGGATAACTC CACCAGTGTA
    CGGGAAGCAG CTGTGGAGCT GCTTGGCCGA TTTGTGCTCT GTCGTCCTCA GCTTGCTGAG
    CAGTATTATG ACATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGAAAAAGA
    GTCATAAAGA TACTTAGGGA TATCTGCATT GAACAACCAA CGTTTCCCAA AATTACAGAG
    ATGTGTGTGA AAATGATTCG GAGAGTCAAT GATGAAGAAG GCATTAAGAA ATTAGTAAAT
    GAGACATTCC AGAAGCTGTG GTTTACTCCA ACTCCACACC ATGACAAAGA AGCAATGACT
    AGGAAAATAC TGAACATCAC TGATGTGGTG GCAGCTTGTA GGGATACAGG GTATGATTGG
    TTTGAGCAGC TTCTTCAAAA TCTATTGAAG TCAGAAGAGG ATGCTTCATA TAAACCTGTG
    AAGAAGGCTT GTACTCAACT TGTTGACAAT TTGGTTGAGC ACATCCTTAA ATATGAGGAG
    TCTTTATCAG ACTCTGACAA CAAAGGAGTG AATTCTGGTC GCTTGGTTGC TTGTATAACC
    ACTCTGTTCT TATTCAGCAA AATCAGGCCC CAGTTAATGG TCAAACATGC CATGACCATG
    CAGCCATATC TGGCAACTAA ATGTAGTACT CAGAATGATT TCATGGTGAT CTGCAATGTT
    GCAAAAATCT TGGAGCTTGT AGTGCCGTTA ATGGAACACC CAAGTGAGAC CTTCCTTGCC
    ACTATTGAGG AAGACCTCAT GAAACTCATC ATCAAATATG GCATGACAGT TGTTCAACAT
    TGTGTGAGCT GCCTTGGGTC TGTTGTGAAC AAGGTCACCC AGAATTACAA GTTTGTGTGG
    GCCTGTTTTA ATAGATACTA TGGTGCACTT TCAAAATTAA AAAGTCAACA CCAAGAAGAC
    CCCAACAGTA CAATACTTAC TGCCAATAAA CCAGCACTCC TTAGATCACT TTTCACTGTT
    GGAGCACTGT GTCGGCATTT TGATTTTGAC CATGAAGATT TTAAGGGCAA CAGTAAGGTT
    AACATTAAGG ATAAAGTACT TGAACTGCTG ATGTATTTTA CAAAGCATTC AGACGAAGAA
    GTACAGACAA AAGCCATTAT TGGCCTGGGA TTTGCATTTA TACAACACCC AAGCTTAATG
    TTTGAACAGG AAGTAAAGAC TCTGTACAAC AGCATTCTTT CAGATAAGAA CTGTTCAGTA
    AACCTAAAAA TCCAGGTGTT AAAAAACCTC CAGACCTACT TGCAGGAGGA GGACACACGT
    ATGCAGCAGG CAGACAGAGA CTGGAAAAAA GTAGCAAAAC AGGAAGACCT GAAAGAAATG
    GGTGATATTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TGTATCTCAA ACAGGTCCTT
    GAGGCTTTCT TTCATACCCA GTCCAATGTA CGCCACTTTG CTCTAAATGT CATTGCACTG
    ACGTTAAACC AGGGTCTCAT CCATCCTGTT CAGTGTGTGC CATACTTAAT TGCTATGGGC
    ACAGATCCAG AGCCTTCTAT GCGAAACAAA GCTGACCAGC AGTTGGTGGA AATAGACAAA
    AAGTATGCTG GTTTCATTCA CATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
    CAACAGGCAA TCAATACCTG CCCAAAGGAT CCTGTAAGAG GCTTTAGACA CGATGAATCA
    TCCAGTGCAT TGTGTTCACA CCTATATTCT ATGATCCGAG GGAACCGTCA ACACAGACGA
    GCATTTCTAA TTTCTTTACT CAATCTCTTC GATGATACTG CAAAAACAGA GGTGAATATG
    CTCTTGTACA TAGCAGACAA TCTAGCCTGT TTTCCTTATC AAACGCAGGA AGAGCCACTG
    TTTATAATGC ATCATATAGA CATTACACTA TCAGTTTCTG GTAGCAACCT ACTACAGTCA
    TTTAAAGAGT CAATGGTGAA AGACAAAAAA AAGGAAAGAA AGCCTTCATC AGATGAGGAG
    ACTGAGAGTG ACAGTAATAG TGGCAGTGAG AGCGAAAGTG AAGAGGAAGC TTCTAAGCCT
    CGGAGGTTAC GAAAACGAGT AGATTCTGAC TCAGATTCTG ATGAAGAAAA TGATATAAAT
    GCTGTGATGA AATGTTTACC AGAAAACTCA GCTCCTTTAA TTGAATTTGC AAATGTGTCT
    CAAGGCATAT TATTACTTTT GATGCTGAAA CAGCATTTAA AGAACCTTTG CGGATTCTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCT GAGTCTGCAA AAGTTTATGA TAAAGCGATA
    AACAGGAAGA CAGGAGTGCA TTTCCATCCA AAACAAACTC TGGATTTTCT ACGGAGTGAT
    ATGGCTAATT CCAAGATCAC TGAAGAAGTG AAGAGGAGTA TAGTAAAACA GTACTTAGAT
    TTCAAGCTCC TTATGGAACA TTTGGATCCT GATGAAGAAG AAGAAGATGG AGAGGTGTCA
    GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AACAATGCAG CTGAGACAGA GGACGATGAA AGTGATGGGG AGGATAGAGG AGGAGGCACT
    TCAGGGGTGA GGCGGAGGAG GAGTCAACGT ATTTCGCAGC GTATTACTCA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004937250.1
    CDS3243..11276
    Translation

    Target ORF information:

    RefSeq Version XM_004937193.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004937193.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGATAAGCCT
    CTGAAGAAGC GAAAACAAGA AAACAAACCA CAGGAGGCTG GGGGTGCTGC TGGAGGAAAT
    AGGCCAGCTT CTCAGGAGTC AGGTTCTGCA GGAAATGGGG CAAGGCCAGC ACTGATGGTT
    AGTATTGATC TTCAACAAGC AGGAAGAGCG GACTCTCAGG CAACAATAAC TCAGGAATTT
    GAGGCTATCA AAAAACCTGA AGAGATTAAG CAGTATAATG ATGGATTTGT GTGTATTCCC
    CAAGAAGAGA CTGTTGGAGT TCTTAAACTT AAACCTGAAA ACCACCCTGA GATTTTTAGG
    AAAAAGTCAG ACTTTGAGGT TCAAAAGACA GATATCCAGC AAAATGAAAA CAGACAAATG
    GAGTTTCAGC AAAATGAGAG CAGAAGGTTG GAAAGTAAAT ACAATGACAA TAAACAGGTA
    GAAGCTAAAT ATGACATCAA GCATGACAGT AGACAAATGG AGATGAAACA AAATGAGAAC
    AGACAGGCAG AGTCAAAACA AAATGAGAGC AGACAAATGG AGATGAAACA AAATGAGGGA
    AAACAAAATA ATGGCAAACA AGAAATAAGA CAGAGACCTG AAACACCAAA GCAGAAGAGC
    GAAGGCAGGC CTGAAACACC CAAGCAGAAG AGTGAAGGCA GGCCTGAAAC ACCAAAACAT
    AGGCATGATA ACAGGAGGGA CTCCAGTAAA CCATTGTCAG AAAAGAAAAT TGAATTATCT
    AGGCATAAAC CTGATGTGAA ATCTGATACT TCAAAGGTAA AATCTGATAG TAGATCAGAT
    CCCGTGAAGC AAAGGCCTGA TGGGCGTTCA GTTTCTGATA CTCAGAGGCG TGACCACGAT
    AGCCTTAAAC AGAGATCTGA GGACAGGGAA TCAGAAAGAT ACAAAGGAGA TCCATCCAAG
    ATTAGAAGGA CTGAATTTTT GAGATCCTCA AACAGAAATG AACATGATTC TAAGTATGGT
    ATTAAATCTG ATGGTTCAAA ACCTGAAAAA TTTGAAAGGA AACACAGACA TGACTCTGGT
    GAATCAAGGG AAAGATTTTC TTCTGGGGAT CAGAAGTCAG ATCAAAAGTC AAGACCTGAC
    AGCCCTCGAG TTAAACAGGA TGGCAAAGGA GATTCTAGCA AATCAAGACC GGATAAACCT
    GGTTTTAGAT CACCCAACAG CAAGGATGAA CGAAGGACAG ACAGTAGTAA GAGTAAAGTA
    GAGAGTAATA AAGCACATCC TGACAATAAA GCAGAGTTTC CCAGTTATCT GCTGGGGGCA
    AGATCTGGTG CACTGAAACA TTTTGTCATT CCAAAAATCA AACGTGACAA AGATGGCAAT
    GTCACTCAGG AGTCAAGGAA AACTGAACTT AAAGGTGAAC AGAAGGACAA AGTAGAGAAG
    ATTGGGCTAG TTGAAGACCT AAATAAAGGA GCTAAGCCTG TAGTTGTCCT TCAGAAGCTT
    TCTTTGGACG ATGTACAAAA ATTTATTAAG GATAGAGAAG ATAAATCAAG GGGCTCTTGT
    TTTAAACCTA ACAAGAACAA GTCGTCCAAA TCTAATAAAG GTAGCATAGA TCAGTCAGTG
    CTAAAGGAGT TGCCTCCTGA GCTCCTGGCA GAAATTGAAT CCACCATGCC TCTTTGTGAA
    CGCGTGAAAA TGAACAAGCG CAAACGTAGC ACAGTTAATG AGAAACCAAA ATATGCTGAA
    ATCAGTTCTG ATGAAGACAA TGACAGTGAA GAAGCTTTTG AATCTTCTCG AAAAAGACAT
    AAAAAGGATA GAGATGAAGC TTGGGAGTAC GAAGAGCATG ACAGAAGAAG TTCTGGTGAC
    CACAGGAGGA GTGGTCATTA TCATGAAGGA AGAAGGACTT CTGGTAGTGG CCGTTACCGC
    AATCGCAGTC CCGATGACTC TGATCTGGAT GATTATTCTC CTCCTCCTAG CCTCAGTGAG
    GTGGCAAGAA AAATGAAGAA AAAAGAAAAG CAAAAGAAGA GGAGAGCTTA CGAGCCTAAA
    CTAACACCTG AAGAAATGAT GGATTCTTCA ACCTTCAAAA GATTTATTGC TTCAATAGAG
    AATATTCTGG AAAATCTGGA AGACATGGAT TTTACAACCT TGGGTGATGA TGATGAGATT
    CCACAGGAAT TGCTTTTGGG AAAACATCAG CTTAATGAGT TGGGTAGTGA ATCTGCAAAA
    ATCAAGGCAA TGGGTATTAT GGACAAGCTC TCAACAGATA AAATAGTAAA GGTCCTGAAC
    ATTTTGGAGA AGAATATTCA AGACGGATCA AAGCTTTCTA CATTACTTAA CCACAACAAT
    GACACTGAAG ATGAGGAGAG ATTATGGAGA GATCTCATAA TGGAGAGAGT AACAAAATCT
    GCAGATGCTT GTCTTACTGC TATCAATATT ATGACATCAC CAAATATGCC TAAAGCTGTT
    TATATTGAGG ACGTAATAGA AAGAGTTATT CAGTACACAA AATTTCATTT GCAGAACACT
    CTCTATCCTC AGTATGACCC TGTGTATAGA GTGGATCCTC TTGGTGGTGG TCTTTTAAGT
    TCAAAAGCAA AACGTGCCAA GTGTTCCACC CACAAACAAA GAGTTATAGT GATGTTGTAT
    AACAAAGTTT GTGATATTGT CAGCAGTTTG TCAGAGTTGC TAGAGATACA ACTTCTTACT
    GATACAACTA TTCTTCAGGT ATCGTCTATG GGGATAACAC CTTTCTTTGT AGAAAATGTC
    AGTGAACTAC AGCTATGTGC CATCAAATTA GTGACTGCAG TCTTCTCCAG GTATGAAAAA
    CATAGACAGC TAATACTAGA AGAAATTTTT ACTTCCCTTG CAAGACTACC AACTAGCAAG
    AGGAGCCTGA GAAACTTCAG GTTGAATAGC AGTGACACAG ATGGAGAGCC TATGTATATT
    CAGATGGTAA CAGCACTAGT TCTGCAGCTT ATTCAATGTG TCGTGCACTT ACCATCAGCA
    GAAAAAGATT CTAATTCAGA TGAGGAATTG AACAAAAAAG TGGATCAAGA TGTGCTTATT
    ACTAACTCAT ATGAAACAGC TATGAGAACA GCACAAAATT TTCTTTCTAT TTTTCTTAAG
    AAATGTGGTA GTAAACAAGG AGAAGAAGAT TACAGGCCTC TCTTTGAGAA CTTCATTCAA
    GATCTTCTTT CCACAGTCAA TAAACCAGAA TGGCCAGCTG CAGAGTTGCT GCTTAGCTTA
    CTAGGAAGGT TATTGGTTCA CCAGTTCAGT AATAAATCAA CAGAAATGGC TTTAAGAGTT
    GCGTCACTTG ACTATCTTGG AACCGTAGCT GCAAGACTGA GGAAAGATGC AGTCACTAGT
    AAAATGGATC AAGGATCTAT AGCTAGAATC CTCAAACAGG CCTCAGGAGG AGAAGATGAT
    ATTCAACAGC TACAGAAGGC TTTATTGGAT TATCTGGATG AGAACACTGA AACTGATGCA
    TCGTTAGTGT TTTCTCGGAA GTTTTATATA GCTCAGTGGT TTCGGGATAC AACTATGGAG
    ACAGAGAAGG CAATTAAATC TCAGAAGGAT GAGGATTCAT CTGAAGGAAC TCATCATGCA
    AAAGATGTTG AGACCACAGG ACAAATCATG CACAGAGCAG AAAGCCGCAA GAAGTTTCTC
    CGCAGCATCA TTAAAACTGC TCCATCTCAG TTCAGTACAT TAAAGATTTA TTCTGACACT
    GTGGACTATG AAGATGCATG TTTGATTGTT CGCTACTTGG CCTCTATGAG GCCGTTTGCC
    CAGAGCTTCG ATATTTATTT AACACAGATT CTGCGTGTAC TTGGTGAAAA TGCAATTGCA
    GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGATCC CAGTATTCTA
    GCCAGGCCGG ATATGCAGCG AGGTGTTCAT GGACGGTTAA TGGATAACTC CACCAGTGTA
    CGGGAAGCAG CTGTGGAGCT GCTTGGCCGA TTTGTGCTCT GTCGTCCTCA GCTTGCTGAG
    CAGTATTATG ACATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGAAAAAGA
    GTCATAAAGA TACTTAGGGA TATCTGCATT GAACAACCAA CGTTTCCCAA AATTACAGAG
    ATGTGTGTGA AAATGATTCG GAGAGTCAAT GATGAAGAAG GCATTAAGAA ATTAGTAAAT
    GAGACATTCC AGAAGCTGTG GTTTACTCCA ACTCCACACC ATGACAAAGA AGCAATGACT
    AGGAAAATAC TGAACATCAC TGATGTGGTG GCAGCTTGTA GGGATACAGG GTATGATTGG
    TTTGAGCAGC TTCTTCAAAA TCTATTGAAG TCAGAAGAGG ATGCTTCATA TAAACCTGTG
    AAGAAGGCTT GTACTCAACT TGTTGACAAT TTGGTTGAGC ACATCCTTAA ATATGAGGAG
    TCTTTATCAG ACTCTGACAA CAAAGGAGTG AATTCTGGTC GCTTGGTTGC TTGTATAACC
    ACTCTGTTCT TATTCAGCAA AATCAGGCCC CAGTTAATGG TCAAACATGC CATGACCATG
    CAGCCATATC TGGCAACTAA ATGTAGTACT CAGAATGATT TCATGGTGAT CTGCAATGTT
    GCAAAAATCT TGGAGCTTGT AGTGCCGTTA ATGGAACACC CAAGTGAGAC CTTCCTTGCC
    ACTATTGAGG AAGACCTCAT GAAACTCATC ATCAAATATG GCATGACAGT TGTTCAACAT
    TGTGTGAGCT GCCTTGGGTC TGTTGTGAAC AAGGTCACCC AGAATTACAA GTTTGTGTGG
    GCCTGTTTTA ATAGATACTA TGGTGCACTT TCAAAATTAA AAAGTCAACA CCAAGAAGAC
    CCCAACAGTA CAATACTTAC TGCCAATAAA CCAGCACTCC TTAGATCACT TTTCACTGTT
    GGAGCACTGT GTCGGCATTT TGATTTTGAC CATGAAGATT TTAAGGGCAA CAGTAAGGTT
    AACATTAAGG ATAAAGTACT TGAACTGCTG ATGTATTTTA CAAAGCATTC AGACGAAGAA
    GTACAGACAA AAGCCATTAT TGGCCTGGGA TTTGCATTTA TACAACACCC AAGCTTAATG
    TTTGAACAGG AAGTAAAGAC TCTGTACAAC AGCATTCTTT CAGATAAGAA CTGTTCAGTA
    AACCTAAAAA TCCAGGTGTT AAAAAACCTC CAGACCTACT TGCAGGAGGA GGACACACGT
    ATGCAGCAGG CAGACAGAGA CTGGAAAAAA GTAGCAAAAC AGGAAGACCT GAAAGAAATG
    GGTGATATTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TGTATCTCAA ACAGGTCCTT
    GAGGCTTTCT TTCATACCCA GTCCAATGTA CGCCACTTTG CTCTAAATGT CATTGCACTG
    ACGTTAAACC AGGGTCTCAT CCATCCTGTT CAGTGTGTGC CATACTTAAT TGCTATGGGC
    ACAGATCCAG AGCCTTCTAT GCGAAACAAA GCTGACCAGC AGTTGGTGGA AATAGACAAA
    AAGTATGCTG GTTTCATTCA CATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
    CAACAGGCAA TCAATACCTG CCCAAAGGAT CCTGTAAGAG GCTTTAGACA CGATGAATCA
    TCCAGTGCAT TGTGTTCACA CCTATATTCT ATGATCCGAG GGAACCGTCA ACACAGACGA
    GCATTTCTAA TTTCTTTACT CAATCTCTTC GATGATACTG CAAAAACAGA GGTGAATATG
    CTCTTGTACA TAGCAGACAA TCTAGCCTGT TTTCCTTATC AAACGCAGGA AGAGCCACTG
    TTTATAATGC ATCATATAGA CATTACACTA TCAGTTTCTG GTAGCAACCT ACTACAGTCA
    TTTAAAGAGT CAATGGTGAA AGACAAAAAA AAGGAAAGAA AGCCTTCATC AGATGAGGAG
    ACTGAGAGTG ACAGTAATAG TGGCAGTGAG AGCGAAAGTG AAGAGGAAGC TTCTAAGCCT
    CGGAGGTTAC GAAAACGAGT AGATTCTGAC TCAGATTCTG ATGAAGAAAA TGATATAAAT
    GCTGTGATGA AATGTTTACC AGAAAACTCA GCTCCTTTAA TTGAATTTGC AAATGTGTCT
    CAAGGCATAT TATTACTTTT GATGCTGAAA CAGCATTTAA AGAACCTTTG CGGATTCTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCT GAGTCTGCAA AAGTTTATGA TAAAGCGATA
    AACAGGAAGA CAGGAGTGCA TTTCCATCCA AAACAAACTC TGGATTTTCT ACGGAGTGAT
    ATGGCTAATT CCAAGATCAC TGAAGAAGTG AAGAGGAGTA TAGTAAAACA GTACTTAGAT
    TTCAAGCTCC TTATGGAACA TTTGGATCCT GATGAAGAAG AAGAAGATGG AGAGGTGTCA
    GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AACAATGCAG CTGAGACAGA GGACGATGAA AGTGATGGGG AGGATAGAGG AGGAGGCACT
    TCAGGGGTGA GGCGGAGGAG GAGTCAACGT ATTTCGCAGC GTATTACTCA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa20947
    Clone ID Related Accession (Same CDS sequence) XM_004937193.3 , XM_004937193.2
    Accession Version XM_004937193.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product nipped-B-like protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004937193.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGATAAGCCT
    CTGAAGAAGC GAAAACAAGA AAACAAACCA CAGGAGGCTG GGGGTGCTGC TGGAGGAAAT
    AGGCCAGCTT CTCAGGAGTC AGGTTCTGCA GGAAATGGGG CAAGGCCAGC ACTGATGGTT
    AGTATTGATC TTCAACAAGC AGGAAGAGCG GACTCTCAGG CAACAATAAC TCAGGAATTT
    GAGGCTATCA AAAAACCTGA AGAGATTAAG CAGTATAATG ATGGATTTGT GTGTATTCCC
    CAAGAAGAGA CTGTTGGAGT TCTTAAACTT AAACCTGAAA ACCACCCTGA GATTTTTAGG
    AAAAAGTCAG ACTTTGAGGT TCAAAAGACA GATATCCAGC AAAATGAAAA CAGACAAATG
    GAGTTTCAGC AAAATGAGAG CAGAAGGTTG GAAAGTAAAT ACAATGACAA TAAACAGGTA
    GAAGCTAAAT ATGACATCAA GCATGACAGT AGACAAATGG AGATGAAACA AAATGAGAAC
    AGACAGGCAG AGTCAAAACA AAATGAGAGC AGACAAATGG AGATGAAACA AAATGAGGGA
    AAACAAAATA ATGGCAAACA AGAAATAAGA CAGAGACCTG AAACACCAAA GCAGAAGAGC
    GAAGGCAGGC CTGAAACACC CAAGCAGAAG AGTGAAGGCA GGCCTGAAAC ACCAAAACAT
    AGGCATGATA ACAGGAGGGA CTCCAGTAAA CCATTGTCAG AAAAGAAAAT TGAATTATCT
    AGGCATAAAC CTGATGTGAA ATCTGATACT TCAAAGGTAA AATCTGATAG TAGATCAGAT
    CCCGTGAAGC AAAGGCCTGA TGGGCGTTCA GTTTCTGATA CTCAGAGGCG TGACCACGAT
    AGCCTTAAAC AGAGATCTGA GGACAGGGAA TCAGAAAGAT ACAAAGGAGA TCCATCCAAG
    ATTAGAAGGA CTGAATTTTT GAGATCCTCA AACAGAAATG AACATGATTC TAAGTATGGT
    ATTAAATCTG ATGGTTCAAA ACCTGAAAAA TTTGAAAGGA AACACAGACA TGACTCTGGT
    GAATCAAGGG AAAGATTTTC TTCTGGGGAT CAGAAGTCAG ATCAAAAGTC AAGACCTGAC
    AGCCCTCGAG TTAAACAGGA TGGCAAAGGA GATTCTAGCA AATCAAGACC GGATAAACCT
    GGTTTTAGAT CACCCAACAG CAAGGATGAA CGAAGGACAG ACAGTAGTAA GAGTAAAGTA
    GAGAGTAATA AAGCACATCC TGACAATAAA GCAGAGTTTC CCAGTTATCT GCTGGGGGCA
    AGATCTGGTG CACTGAAACA TTTTGTCATT CCAAAAATCA AACGTGACAA AGATGGCAAT
    GTCACTCAGG AGTCAAGGAA AACTGAACTT AAAGGTGAAC AGAAGGACAA AGTAGAGAAG
    ATTGGGCTAG TTGAAGACCT AAATAAAGGA GCTAAGCCTG TAGTTGTCCT TCAGAAGCTT
    TCTTTGGACG ATGTACAAAA ATTTATTAAG GATAGAGAAG ATAAATCAAG GGGCTCTTGT
    TTTAAACCTA ACAAGAACAA GTCGTCCAAA TCTAATAAAG GTAGCATAGA TCAGTCAGTG
    CTAAAGGAGT TGCCTCCTGA GCTCCTGGCA GAAATTGAAT CCACCATGCC TCTTTGTGAA
    CGCGTGAAAA TGAACAAGCG CAAACGTAGC ACAGTTAATG AGAAACCAAA ATATGCTGAA
    ATCAGTTCTG ATGAAGACAA TGACAGTGAA GAAGCTTTTG AATCTTCTCG AAAAAGACAT
    AAAAAGGATA GAGATGAAGC TTGGGAGTAC GAAGAGCATG ACAGAAGAAG TTCTGGTGAC
    CACAGGAGGA GTGGTCATTA TCATGAAGGA AGAAGGACTT CTGGTAGTGG CCGTTACCGC
    AATCGCAGTC CCGATGACTC TGATCTGGAT GATTATTCTC CTCCTCCTAG CCTCAGTGAG
    GTGGCAAGAA AAATGAAGAA AAAAGAAAAG CAAAAGAAGA GGAGAGCTTA CGAGCCTAAA
    CTAACACCTG AAGAAATGAT GGATTCTTCA ACCTTCAAAA GATTTATTGC TTCAATAGAG
    AATATTCTGG AAAATCTGGA AGACATGGAT TTTACAACCT TGGGTGATGA TGATGAGATT
    CCACAGGAAT TGCTTTTGGG AAAACATCAG CTTAATGAGT TGGGTAGTGA ATCTGCAAAA
    ATCAAGGCAA TGGGTATTAT GGACAAGCTC TCAACAGATA AAATAGTAAA GGTCCTGAAC
    ATTTTGGAGA AGAATATTCA AGACGGATCA AAGCTTTCTA CATTACTTAA CCACAACAAT
    GACACTGAAG ATGAGGAGAG ATTATGGAGA GATCTCATAA TGGAGAGAGT AACAAAATCT
    GCAGATGCTT GTCTTACTGC TATCAATATT ATGACATCAC CAAATATGCC TAAAGCTGTT
    TATATTGAGG ACGTAATAGA AAGAGTTATT CAGTACACAA AATTTCATTT GCAGAACACT
    CTCTATCCTC AGTATGACCC TGTGTATAGA GTGGATCCTC TTGGTGGTGG TCTTTTAAGT
    TCAAAAGCAA AACGTGCCAA GTGTTCCACC CACAAACAAA GAGTTATAGT GATGTTGTAT
    AACAAAGTTT GTGATATTGT CAGCAGTTTG TCAGAGTTGC TAGAGATACA ACTTCTTACT
    GATACAACTA TTCTTCAGGT ATCGTCTATG GGGATAACAC CTTTCTTTGT AGAAAATGTC
    AGTGAACTAC AGCTATGTGC CATCAAATTA GTGACTGCAG TCTTCTCCAG GTATGAAAAA
    CATAGACAGC TAATACTAGA AGAAATTTTT ACTTCCCTTG CAAGACTACC AACTAGCAAG
    AGGAGCCTGA GAAACTTCAG GTTGAATAGC AGTGACACAG ATGGAGAGCC TATGTATATT
    CAGATGGTAA CAGCACTAGT TCTGCAGCTT ATTCAATGTG TCGTGCACTT ACCATCAGCA
    GAAAAAGATT CTAATTCAGA TGAGGAATTG AACAAAAAAG TGGATCAAGA TGTGCTTATT
    ACTAACTCAT ATGAAACAGC TATGAGAACA GCACAAAATT TTCTTTCTAT TTTTCTTAAG
    AAATGTGGTA GTAAACAAGG AGAAGAAGAT TACAGGCCTC TCTTTGAGAA CTTCATTCAA
    GATCTTCTTT CCACAGTCAA TAAACCAGAA TGGCCAGCTG CAGAGTTGCT GCTTAGCTTA
    CTAGGAAGGT TATTGGTTCA CCAGTTCAGT AATAAATCAA CAGAAATGGC TTTAAGAGTT
    GCGTCACTTG ACTATCTTGG AACCGTAGCT GCAAGACTGA GGAAAGATGC AGTCACTAGT
    AAAATGGATC AAGGATCTAT AGCTAGAATC CTCAAACAGG CCTCAGGAGG AGAAGATGAT
    ATTCAACAGC TACAGAAGGC TTTATTGGAT TATCTGGATG AGAACACTGA AACTGATGCA
    TCGTTAGTGT TTTCTCGGAA GTTTTATATA GCTCAGTGGT TTCGGGATAC AACTATGGAG
    ACAGAGAAGG CAATTAAATC TCAGAAGGAT GAGGATTCAT CTGAAGGAAC TCATCATGCA
    AAAGATGTTG AGACCACAGG ACAAATCATG CACAGAGCAG AAAGCCGCAA GAAGTTTCTC
    CGCAGCATCA TTAAAACTGC TCCATCTCAG TTCAGTACAT TAAAGATTTA TTCTGACACT
    GTGGACTATG AAGATGCATG TTTGATTGTT CGCTACTTGG CCTCTATGAG GCCGTTTGCC
    CAGAGCTTCG ATATTTATTT AACACAGATT CTGCGTGTAC TTGGTGAAAA TGCAATTGCA
    GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGATCC CAGTATTCTA
    GCCAGGCCGG ATATGCAGCG AGGTGTTCAT GGACGGTTAA TGGATAACTC CACCAGTGTA
    CGGGAAGCAG CTGTGGAGCT GCTTGGCCGA TTTGTGCTCT GTCGTCCTCA GCTTGCTGAG
    CAGTATTATG ACATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGAAAAAGA
    GTCATAAAGA TACTTAGGGA TATCTGCATT GAACAACCAA CGTTTCCCAA AATTACAGAG
    ATGTGTGTGA AAATGATTCG GAGAGTCAAT GATGAAGAAG GCATTAAGAA ATTAGTAAAT
    GAGACATTCC AGAAGCTGTG GTTTACTCCA ACTCCACACC ATGACAAAGA AGCAATGACT
    AGGAAAATAC TGAACATCAC TGATGTGGTG GCAGCTTGTA GGGATACAGG GTATGATTGG
    TTTGAGCAGC TTCTTCAAAA TCTATTGAAG TCAGAAGAGG ATGCTTCATA TAAACCTGTG
    AAGAAGGCTT GTACTCAACT TGTTGACAAT TTGGTTGAGC ACATCCTTAA ATATGAGGAG
    TCTTTATCAG ACTCTGACAA CAAAGGAGTG AATTCTGGTC GCTTGGTTGC TTGTATAACC
    ACTCTGTTCT TATTCAGCAA AATCAGGCCC CAGTTAATGG TCAAACATGC CATGACCATG
    CAGCCATATC TGGCAACTAA ATGTAGTACT CAGAATGATT TCATGGTGAT CTGCAATGTT
    GCAAAAATCT TGGAGCTTGT AGTGCCGTTA ATGGAACACC CAAGTGAGAC CTTCCTTGCC
    ACTATTGAGG AAGACCTCAT GAAACTCATC ATCAAATATG GCATGACAGT TGTTCAACAT
    TGTGTGAGCT GCCTTGGGTC TGTTGTGAAC AAGGTCACCC AGAATTACAA GTTTGTGTGG
    GCCTGTTTTA ATAGATACTA TGGTGCACTT TCAAAATTAA AAAGTCAACA CCAAGAAGAC
    CCCAACAGTA CAATACTTAC TGCCAATAAA CCAGCACTCC TTAGATCACT TTTCACTGTT
    GGAGCACTGT GTCGGCATTT TGATTTTGAC CATGAAGATT TTAAGGGCAA CAGTAAGGTT
    AACATTAAGG ATAAAGTACT TGAACTGCTG ATGTATTTTA CAAAGCATTC AGACGAAGAA
    GTACAGACAA AAGCCATTAT TGGCCTGGGA TTTGCATTTA TACAACACCC AAGCTTAATG
    TTTGAACAGG AAGTAAAGAC TCTGTACAAC AGCATTCTTT CAGATAAGAA CTGTTCAGTA
    AACCTAAAAA TCCAGGTGTT AAAAAACCTC CAGACCTACT TGCAGGAGGA GGACACACGT
    ATGCAGCAGG CAGACAGAGA CTGGAAAAAA GTAGCAAAAC AGGAAGACCT GAAAGAAATG
    GGTGATATTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TGTATCTCAA ACAGGTCCTT
    GAGGCTTTCT TTCATACCCA GTCCAATGTA CGCCACTTTG CTCTAAATGT CATTGCACTG
    ACGTTAAACC AGGGTCTCAT CCATCCTGTT CAGTGTGTGC CATACTTAAT TGCTATGGGC
    ACAGATCCAG AGCCTTCTAT GCGAAACAAA GCTGACCAGC AGTTGGTGGA AATAGACAAA
    AAGTATGCTG GTTTCATTCA CATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
    CAACAGGCAA TCAATACCTG CCCAAAGGAT CCTGTAAGAG GCTTTAGACA CGATGAATCA
    TCCAGTGCAT TGTGTTCACA CCTATATTCT ATGATCCGAG GGAACCGTCA ACACAGACGA
    GCATTTCTAA TTTCTTTACT CAATCTCTTC GATGATACTG CAAAAACAGA GGTGAATATG
    CTCTTGTACA TAGCAGACAA TCTAGCCTGT TTTCCTTATC AAACGCAGGA AGAGCCACTG
    TTTATAATGC ATCATATAGA CATTACACTA TCAGTTTCTG GTAGCAACCT ACTACAGTCA
    TTTAAAGAGT CAATGGTGAA AGACAAAAAA AAGGAAAGAA AGCCTTCATC AGATGAGGAG
    ACTGAGAGTG ACAGTAATAG TGGCAGTGAG AGCGAAAGTG AAGAGGAAGC TTCTAAGCCT
    CGGAGGTTAC GAAAACGAGT AGATTCTGAC TCAGATTCTG ATGAAGAAAA TGATATAAAT
    GCTGTGATGA AATGTTTACC AGAAAACTCA GCTCCTTTAA TTGAATTTGC AAATGTGTCT
    CAAGGCATAT TATTACTTTT GATGCTGAAA CAGCATTTAA AGAACCTTTG CGGATTCTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCT GAGTCTGCAA AAGTTTATGA TAAAGCGATA
    AACAGGAAGA CAGGAGTGCA TTTCCATCCA AAACAAACTC TGGATTTTCT ACGGAGTGAT
    ATGGCTAATT CCAAGATCAC TGAAGAAGTG AAGAGGAGTA TAGTAAAACA GTACTTAGAT
    TTCAAGCTCC TTATGGAACA TTTGGATCCT GATGAAGAAG AAGAAGATGG AGAGGTGTCA
    GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AACAATGCAG CTGAGACAGA GGACGATGAA AGTGATGGGG AGGATAGAGG AGGAGGCACT
    TCAGGGGTGA GGCGGAGGAG GAGTCAACGT ATTTCGCAGC GTATTACTCA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004937250.1
    CDS3252..11285
    Translation

    Target ORF information:

    RefSeq Version XM_004937193.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004937193.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGG ATCAAGACCA CCTTTAATCC TACAATCTCA GCCTCCACCT
    TATTCATCAC CTAGAGATGT TCCCCCAGAC ATATTGTTAG ATTCTCCAGA AAGAAAGCAA
    AAGAAACAAA AGAAAATGAA GTTAGGCAAG GATGAAAAAG ACCAGACTGA AAAAGCTGCA
    ATGTATGATA TCATTAGTTC TCCTTCCAAG GACTCCACTA AGCTTACATT AAGACTCTCT
    CGTGTAAGAT CTTCGGATTT GGACCAGCAG GATGATATGC TTTCTGGTTT GGAAAACAGC
    AGTGTGTCAG AGGGTGATAT TCCTTTTAAT GTGCAGTACC CAGGACAGAC TTCTAAAACA
    CCCGTTACTC CACAGGATGT TAACCGCCCA CTAAACGCTG CTCAGTGTTT GCCGCAGCAT
    GAACAGACAG CTTTCCTTCA GGCACAACAA GTGCCTGTTT TACAACAGAA CACTTCAGTT
    GCTGCAAAAC AACCTCAAAC TCCTGTGGTA CAGACACAGC AACAGGTTTC GCAACAAGGA
    ACTATAACGT CATATGATGA AGTAGAATTG GATGCATTGG CTGAAATTGA GCGGATAGAA
    CGTGAATCAG CTATTGAAAG GGAGCGATTT TCAAAAGAAG TGCAAGATAA AGATAAGCCT
    CTGAAGAAGC GAAAACAAGA AAACAAACCA CAGGAGGCTG GGGGTGCTGC TGGAGGAAAT
    AGGCCAGCTT CTCAGGAGTC AGGTTCTGCA GGAAATGGGG CAAGGCCAGC ACTGATGGTT
    AGTATTGATC TTCAACAAGC AGGAAGAGCG GACTCTCAGG CAACAATAAC TCAGGAATTT
    GAGGCTATCA AAAAACCTGA AGAGATTAAG CAGTATAATG ATGGATTTGT GTGTATTCCC
    CAAGAAGAGA CTGTTGGAGT TCTTAAACTT AAACCTGAAA ACCACCCTGA GATTTTTAGG
    AAAAAGTCAG ACTTTGAGGT TCAAAAGACA GATATCCAGC AAAATGAAAA CAGACAAATG
    GAGTTTCAGC AAAATGAGAG CAGAAGGTTG GAAAGTAAAT ACAATGACAA TAAACAGGTA
    GAAGCTAAAT ATGACATCAA GCATGACAGT AGACAAATGG AGATGAAACA AAATGAGAAC
    AGACAGGCAG AGTCAAAACA AAATGAGAGC AGACAAATGG AGATGAAACA AAATGAGGGA
    AAACAAAATA ATGGCAAACA AGAAATAAGA CAGAGACCTG AAACACCAAA GCAGAAGAGC
    GAAGGCAGGC CTGAAACACC CAAGCAGAAG AGTGAAGGCA GGCCTGAAAC ACCAAAACAT
    AGGCATGATA ACAGGAGGGA CTCCAGTAAA CCATTGTCAG AAAAGAAAAT TGAATTATCT
    AGGCATAAAC CTGATGTGAA ATCTGATACT TCAAAGGTAA AATCTGATAG TAGATCAGAT
    CCCGTGAAGC AAAGGCCTGA TGGGCGTTCA GTTTCTGATA CTCAGAGGCG TGACCACGAT
    AGCCTTAAAC AGAGATCTGA GGACAGGGAA TCAGAAAGAT ACAAAGGAGA TCCATCCAAG
    ATTAGAAGGA CTGAATTTTT GAGATCCTCA AACAGAAATG AACATGATTC TAAGTATGGT
    ATTAAATCTG ATGGTTCAAA ACCTGAAAAA TTTGAAAGGA AACACAGACA TGACTCTGGT
    GAATCAAGGG AAAGATTTTC TTCTGGGGAT CAGAAGTCAG ATCAAAAGTC AAGACCTGAC
    AGCCCTCGAG TTAAACAGGA TGGCAAAGGA GATTCTAGCA AATCAAGACC GGATAAACCT
    GGTTTTAGAT CACCCAACAG CAAGGATGAA CGAAGGACAG ACAGTAGTAA GAGTAAAGTA
    GAGAGTAATA AAGCACATCC TGACAATAAA GCAGAGTTTC CCAGTTATCT GCTGGGGGCA
    AGATCTGGTG CACTGAAACA TTTTGTCATT CCAAAAATCA AACGTGACAA AGATGGCAAT
    GTCACTCAGG AGTCAAGGAA AACTGAACTT AAAGGTGAAC AGAAGGACAA AGTAGAGAAG
    ATTGGGCTAG TTGAAGACCT AAATAAAGGA GCTAAGCCTG TAGTTGTCCT TCAGAAGCTT
    TCTTTGGACG ATGTACAAAA ATTTATTAAG GATAGAGAAG ATAAATCAAG GGGCTCTTGT
    TTTAAACCTA ACAAGAACAA GTCGTCCAAA TCTAATAAAG GTAGCATAGA TCAGTCAGTG
    CTAAAGGAGT TGCCTCCTGA GCTCCTGGCA GAAATTGAAT CCACCATGCC TCTTTGTGAA
    CGCGTGAAAA TGAACAAGCG CAAACGTAGC ACAGTTAATG AGAAACCAAA ATATGCTGAA
    ATCAGTTCTG ATGAAGACAA TGACAGTGAA GAAGCTTTTG AATCTTCTCG AAAAAGACAT
    AAAAAGGATA GAGATGAAGC TTGGGAGTAC GAAGAGCATG ACAGAAGAAG TTCTGGTGAC
    CACAGGAGGA GTGGTCATTA TCATGAAGGA AGAAGGACTT CTGGTAGTGG CCGTTACCGC
    AATCGCAGTC CCGATGACTC TGATCTGGAT GATTATTCTC CTCCTCCTAG CCTCAGTGAG
    GTGGCAAGAA AAATGAAGAA AAAAGAAAAG CAAAAGAAGA GGAGAGCTTA CGAGCCTAAA
    CTAACACCTG AAGAAATGAT GGATTCTTCA ACCTTCAAAA GATTTATTGC TTCAATAGAG
    AATATTCTGG AAAATCTGGA AGACATGGAT TTTACAACCT TGGGTGATGA TGATGAGATT
    CCACAGGAAT TGCTTTTGGG AAAACATCAG CTTAATGAGT TGGGTAGTGA ATCTGCAAAA
    ATCAAGGCAA TGGGTATTAT GGACAAGCTC TCAACAGATA AAATAGTAAA GGTCCTGAAC
    ATTTTGGAGA AGAATATTCA AGACGGATCA AAGCTTTCTA CATTACTTAA CCACAACAAT
    GACACTGAAG ATGAGGAGAG ATTATGGAGA GATCTCATAA TGGAGAGAGT AACAAAATCT
    GCAGATGCTT GTCTTACTGC TATCAATATT ATGACATCAC CAAATATGCC TAAAGCTGTT
    TATATTGAGG ACGTAATAGA AAGAGTTATT CAGTACACAA AATTTCATTT GCAGAACACT
    CTCTATCCTC AGTATGACCC TGTGTATAGA GTGGATCCTC TTGGTGGTGG TCTTTTAAGT
    TCAAAAGCAA AACGTGCCAA GTGTTCCACC CACAAACAAA GAGTTATAGT GATGTTGTAT
    AACAAAGTTT GTGATATTGT CAGCAGTTTG TCAGAGTTGC TAGAGATACA ACTTCTTACT
    GATACAACTA TTCTTCAGGT ATCGTCTATG GGGATAACAC CTTTCTTTGT AGAAAATGTC
    AGTGAACTAC AGCTATGTGC CATCAAATTA GTGACTGCAG TCTTCTCCAG GTATGAAAAA
    CATAGACAGC TAATACTAGA AGAAATTTTT ACTTCCCTTG CAAGACTACC AACTAGCAAG
    AGGAGCCTGA GAAACTTCAG GTTGAATAGC AGTGACACAG ATGGAGAGCC TATGTATATT
    CAGATGGTAA CAGCACTAGT TCTGCAGCTT ATTCAATGTG TCGTGCACTT ACCATCAGCA
    GAAAAAGATT CTAATTCAGA TGAGGAATTG AACAAAAAAG TGGATCAAGA TGTGCTTATT
    ACTAACTCAT ATGAAACAGC TATGAGAACA GCACAAAATT TTCTTTCTAT TTTTCTTAAG
    AAATGTGGTA GTAAACAAGG AGAAGAAGAT TACAGGCCTC TCTTTGAGAA CTTCATTCAA
    GATCTTCTTT CCACAGTCAA TAAACCAGAA TGGCCAGCTG CAGAGTTGCT GCTTAGCTTA
    CTAGGAAGGT TATTGGTTCA CCAGTTCAGT AATAAATCAA CAGAAATGGC TTTAAGAGTT
    GCGTCACTTG ACTATCTTGG AACCGTAGCT GCAAGACTGA GGAAAGATGC AGTCACTAGT
    AAAATGGATC AAGGATCTAT AGCTAGAATC CTCAAACAGG CCTCAGGAGG AGAAGATGAT
    ATTCAACAGC TACAGAAGGC TTTATTGGAT TATCTGGATG AGAACACTGA AACTGATGCA
    TCGTTAGTGT TTTCTCGGAA GTTTTATATA GCTCAGTGGT TTCGGGATAC AACTATGGAG
    ACAGAGAAGG CAATTAAATC TCAGAAGGAT GAGGATTCAT CTGAAGGAAC TCATCATGCA
    AAAGATGTTG AGACCACAGG ACAAATCATG CACAGAGCAG AAAGCCGCAA GAAGTTTCTC
    CGCAGCATCA TTAAAACTGC TCCATCTCAG TTCAGTACAT TAAAGATTTA TTCTGACACT
    GTGGACTATG AAGATGCATG TTTGATTGTT CGCTACTTGG CCTCTATGAG GCCGTTTGCC
    CAGAGCTTCG ATATTTATTT AACACAGATT CTGCGTGTAC TTGGTGAAAA TGCAATTGCA
    GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGATCC CAGTATTCTA
    GCCAGGCCGG ATATGCAGCG AGGTGTTCAT GGACGGTTAA TGGATAACTC CACCAGTGTA
    CGGGAAGCAG CTGTGGAGCT GCTTGGCCGA TTTGTGCTCT GTCGTCCTCA GCTTGCTGAG
    CAGTATTATG ACATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGAAAAAGA
    GTCATAAAGA TACTTAGGGA TATCTGCATT GAACAACCAA CGTTTCCCAA AATTACAGAG
    ATGTGTGTGA AAATGATTCG GAGAGTCAAT GATGAAGAAG GCATTAAGAA ATTAGTAAAT
    GAGACATTCC AGAAGCTGTG GTTTACTCCA ACTCCACACC ATGACAAAGA AGCAATGACT
    AGGAAAATAC TGAACATCAC TGATGTGGTG GCAGCTTGTA GGGATACAGG GTATGATTGG
    TTTGAGCAGC TTCTTCAAAA TCTATTGAAG TCAGAAGAGG ATGCTTCATA TAAACCTGTG
    AAGAAGGCTT GTACTCAACT TGTTGACAAT TTGGTTGAGC ACATCCTTAA ATATGAGGAG
    TCTTTATCAG ACTCTGACAA CAAAGGAGTG AATTCTGGTC GCTTGGTTGC TTGTATAACC
    ACTCTGTTCT TATTCAGCAA AATCAGGCCC CAGTTAATGG TCAAACATGC CATGACCATG
    CAGCCATATC TGGCAACTAA ATGTAGTACT CAGAATGATT TCATGGTGAT CTGCAATGTT
    GCAAAAATCT TGGAGCTTGT AGTGCCGTTA ATGGAACACC CAAGTGAGAC CTTCCTTGCC
    ACTATTGAGG AAGACCTCAT GAAACTCATC ATCAAATATG GCATGACAGT TGTTCAACAT
    TGTGTGAGCT GCCTTGGGTC TGTTGTGAAC AAGGTCACCC AGAATTACAA GTTTGTGTGG
    GCCTGTTTTA ATAGATACTA TGGTGCACTT TCAAAATTAA AAAGTCAACA CCAAGAAGAC
    CCCAACAGTA CAATACTTAC TGCCAATAAA CCAGCACTCC TTAGATCACT TTTCACTGTT
    GGAGCACTGT GTCGGCATTT TGATTTTGAC CATGAAGATT TTAAGGGCAA CAGTAAGGTT
    AACATTAAGG ATAAAGTACT TGAACTGCTG ATGTATTTTA CAAAGCATTC AGACGAAGAA
    GTACAGACAA AAGCCATTAT TGGCCTGGGA TTTGCATTTA TACAACACCC AAGCTTAATG
    TTTGAACAGG AAGTAAAGAC TCTGTACAAC AGCATTCTTT CAGATAAGAA CTGTTCAGTA
    AACCTAAAAA TCCAGGTGTT AAAAAACCTC CAGACCTACT TGCAGGAGGA GGACACACGT
    ATGCAGCAGG CAGACAGAGA CTGGAAAAAA GTAGCAAAAC AGGAAGACCT GAAAGAAATG
    GGTGATATTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TGTATCTCAA ACAGGTCCTT
    GAGGCTTTCT TTCATACCCA GTCCAATGTA CGCCACTTTG CTCTAAATGT CATTGCACTG
    ACGTTAAACC AGGGTCTCAT CCATCCTGTT CAGTGTGTGC CATACTTAAT TGCTATGGGC
    ACAGATCCAG AGCCTTCTAT GCGAAACAAA GCTGACCAGC AGTTGGTGGA AATAGACAAA
    AAGTATGCTG GTTTCATTCA CATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
    CAACAGGCAA TCAATACCTG CCCAAAGGAT CCTGTAAGAG GCTTTAGACA CGATGAATCA
    TCCAGTGCAT TGTGTTCACA CCTATATTCT ATGATCCGAG GGAACCGTCA ACACAGACGA
    GCATTTCTAA TTTCTTTACT CAATCTCTTC GATGATACTG CAAAAACAGA GGTGAATATG
    CTCTTGTACA TAGCAGACAA TCTAGCCTGT TTTCCTTATC AAACGCAGGA AGAGCCACTG
    TTTATAATGC ATCATATAGA CATTACACTA TCAGTTTCTG GTAGCAACCT ACTACAGTCA
    TTTAAAGAGT CAATGGTGAA AGACAAAAAA AAGGAAAGAA AGCCTTCATC AGATGAGGAG
    ACTGAGAGTG ACAGTAATAG TGGCAGTGAG AGCGAAAGTG AAGAGGAAGC TTCTAAGCCT
    CGGAGGTTAC GAAAACGAGT AGATTCTGAC TCAGATTCTG ATGAAGAAAA TGATATAAAT
    GCTGTGATGA AATGTTTACC AGAAAACTCA GCTCCTTTAA TTGAATTTGC AAATGTGTCT
    CAAGGCATAT TATTACTTTT GATGCTGAAA CAGCATTTAA AGAACCTTTG CGGATTCTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCT GAGTCTGCAA AAGTTTATGA TAAAGCGATA
    AACAGGAAGA CAGGAGTGCA TTTCCATCCA AAACAAACTC TGGATTTTCT ACGGAGTGAT
    ATGGCTAATT CCAAGATCAC TGAAGAAGTG AAGAGGAGTA TAGTAAAACA GTACTTAGAT
    TTCAAGCTCC TTATGGAACA TTTGGATCCT GATGAAGAAG AAGAAGATGG AGAGGTGTCA
    GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AACAATGCAG CTGAGACAGA GGACGATGAA AGTGATGGGG AGGATAGAGG AGGAGGCACT
    TCAGGGGTGA GGCGGAGGAG GAGTCAACGT ATTTCGCAGC GTATTACTCA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa20948
    Clone ID Related Accession (Same CDS sequence) XM_015277521.1 , XM_015277521.2
    Accession Version XM_015277521.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7719bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product nipped-B-like protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGA TAAGCCTCTG AAGAAGCGAA AACAAGAAAA CAAACCACAG
    GAGGCTGGGG GTGCTGCTGG AGGAAATAGG CCAGCTTCTC AGGAGTCAGG TTCTGCAGGA
    AATGGGGCAA GGCCAGCACT GATGGTTAGT ATTGATCTTC AACAAGCAGG AAGAGCGGAC
    TCTCAGGCAA CAATAACTCA GGAATTTGAG GCTATCAAAA AACCTGAAGA GATTAAGCAG
    TATAATGATG GATTTGTGTG TATTCCCCAA GAAGAGACTG TTGGAGTTCT TAAACTTAAA
    CCTGAAAACC ACCCTGAGAT TTTTAGGAAA AAGTCAGACT TTGAGGTTCA AAAGACAGAT
    ATCCAGCAAA ATGAAAACAG ACAAATGGAG TTTCAGCAAA ATGAGAGCAG AAGGTTGGAA
    AGTAAATACA ATGACAATAA ACAGGTAGAA GCTAAATATG ACATCAAGCA TGACAGTAGA
    CAAATGGAGA TGAAACAAAA TGAGAACAGA CAGGCAGAGT CAAAACAAAA TGAGAGCAGA
    CAAATGGAGA TGAAACAAAA TGAGGGAAAA CAAAATAATG GCAAACAAGA AATAAGACAG
    AGACCTGAAA CACCAAAGCA GAAGAGCGAA GGCAGGCCTG AAACACCCAA GCAGAAGAGT
    GAAGGCAGGC CTGAAACACC AAAACATAGG CATGATAACA GGAGGGACTC CAGTAAACCA
    TTGTCAGAAA AGAAAATTGA ATTATCTAGG CATAAACCTG ATGTGAAATC TGATACTTCA
    AAGGTAAAAT CTGATAGTAG ATCAGATCCC GTGAAGCAAA GGCCTGATGG GCGTTCAGTT
    TCTGATACTC AGAGGCGTGA CCACGATAGC CTTAAACAGA GATCTGAGGA CAGGGAATCA
    GAAAGATACA AAGGAGATCC ATCCAAGATT AGAAGGACTG AATTTTTGAG ATCCTCAAAC
    AGAAATGAAC ATGATTCTAA GTATGGTATT AAATCTGATG GTTCAAAACC TGAAAAATTT
    GAAAGGAAAC ACAGACATGA CTCTGGTGAA TCAAGGGAAA GATTTTCTTC TGGGGATCAG
    AAGTCAGATC AAAAGTCAAG ACCTGACAGC CCTCGAGTTA AACAGGATGG CAAAGGAGAT
    TCTAGCAAAT CAAGACCGGA TAAACCTGGT TTTAGATCAC CCAACAGCAA GGATGAACGA
    AGGACAGACA GTAGTAAGAG TAAAGTAGAG AGTAATAAAG CACATCCTGA CAATAAAGCA
    GAGTTTCCCA GTTATCTGCT GGGGGCAAGA TCTGGTGCAC TGAAACATTT TGTCATTCCA
    AAAATCAAAC GTGACAAAGA TGGCAATGTC ACTCAGGAGT CAAGGAAAAC TGAACTTAAA
    GGTGAACAGA AGGACAAAGT AGAGAAGATT GGGCTAGTTG AAGACCTAAA TAAAGGAGCT
    AAGCCTGTAG TTGTCCTTCA GAAGCTTTCT TTGGACGATG TACAAAAATT TATTAAGGAT
    AGAGAAGATA AATCAAGGGG CTCTTGTTTT AAACCTAACA AGAACAAGTC GTCCAAATCT
    AATAAAGGTA GCATAGATCA GTCAGTGCTA AAGGAGTTGC CTCCTGAGCT CCTGGCAGAA
    ATTGAATCCA CCATGCCTCT TTGTGAACGC GTGAAAATGA ACAAGCGCAA ACGTAGCACA
    GTTAATGAGA AACCAAAATA TGCTGAAATC AGTTCTGATG AAGACAATGA CAGTGAAGAA
    GCTTTTGAAT CTTCTCGAAA AAGACATAAA AAGGATAGAG ATGAAGCTTG GGAGTACGAA
    GAGCATGACA GAAGAAGTTC TGGTGACCAC AGGAGGAGTG GTCATTATCA TGAAGGAAGA
    AGGACTTCTG GTAGTGGCCG TTACCGCAAT CGCAGTCCCG ATGACTCTGA TCTGGATGAT
    TATTCTCCTC CTCCTAGCCT CAGTGAGGTG GCAAGAAAAA TGAAGAAAAA AGAAAAGCAA
    AAGAAGAGGA GAGCTTACGA GCCTAAACTA ACACCTGAAG AAATGATGGA TTCTTCAACC
    TTCAAAAGAT TTATTGCTTC AATAGAGAAT ATTCTGGAAA ATCTGGAAGA CATGGATTTT
    ACAACCTTGG GTGATGATGA TGAGATTCCA CAGGAATTGC TTTTGGGAAA ACATCAGCTT
    AATGAGTTGG GTAGTGAATC TGCAAAAATC AAGGCAATGG GTATTATGGA CAAGCTCTCA
    ACAGATAAAA TAGTAAAGGT CCTGAACATT TTGGAGAAGA ATATTCAAGA CGGATCAAAG
    CTTTCTACAT TACTTAACCA CAACAATGAC ACTGAAGATG AGGAGAGATT ATGGAGAGAT
    CTCATAATGG AGAGAGTAAC AAAATCTGCA GATGCTTGTC TTACTGCTAT CAATATTATG
    ACATCACCAA ATATGCCTAA AGCTGTTTAT ATTGAGGACG TAATAGAAAG AGTTATTCAG
    TACACAAAAT TTCATTTGCA GAACACTCTC TATCCTCAGT ATGACCCTGT GTATAGAGTG
    GATCCTCTTG GTGGTGGTCT TTTAAGTTCA AAAGCAAAAC GTGCCAAGTG TTCCACCCAC
    AAACAAAGAG TTATAGTGAT GTTGTATAAC AAAGTTTGTG ATATTGTCAG CAGTTTGTCA
    GAGTTGCTAG AGATACAACT TCTTACTGAT ACAACTATTC TTCAGGTATC GTCTATGGGG
    ATAACACCTT TCTTTGTAGA AAATGTCAGT GAACTACAGC TATGTGCCAT CAAATTAGTG
    ACTGCAGTCT TCTCCAGGTA TGAAAAACAT AGACAGCTAA TACTAGAAGA AATTTTTACT
    TCCCTTGCAA GACTACCAAC TAGCAAGAGG AGCCTGAGAA ACTTCAGGTT GAATAGCAGT
    GACACAGATG GAGAGCCTAT GTATATTCAG ATGGTAACAG CACTAGTTCT GCAGCTTATT
    CAATGTGTCG TGCACTTACC ATCAGCAGAA AAAGATTCTA ATTCAGATGA GGAATTGAAC
    AAAAAAGTGG ATCAAGATGT GCTTATTACT AACTCATATG AAACAGCTAT GAGAACAGCA
    CAAAATTTTC TTTCTATTTT TCTTAAGAAA TGTGGTAGTA AACAAGGAGA AGAAGATTAC
    AGGCCTCTCT TTGAGAACTT CATTCAAGAT CTTCTTTCCA CAGTCAATAA ACCAGAATGG
    CCAGCTGCAG AGTTGCTGCT TAGCTTACTA GGAAGGTTAT TGGTTCACCA GTTCAGTAAT
    AAATCAACAG AAATGGCTTT AAGAGTTGCG TCACTTGACT ATCTTGGAAC CGTAGCTGCA
    AGACTGAGGA AAGATGCAGT CACTAGTAAA ATGGATCAAG GATCTATAGC TAGAATCCTC
    AAACAGGCCT CAGGAGGAGA AGATGATATT CAACAGCTAC AGAAGGCTTT ATTGGATTAT
    CTGGATGAGA ACACTGAAAC TGATGCATCG TTAGTGTTTT CTCGGAAGTT TTATATAGCT
    CAGTGGTTTC GGGATACAAC TATGGAGACA GAGAAGGCAA TTAAATCTCA GAAGGATGAG
    GATTCATCTG AAGGAACTCA TCATGCAAAA GATGTTGAGA CCACAGGACA AATCATGCAC
    AGAGCAGAAA GCCGCAAGAA GTTTCTCCGC AGCATCATTA AAACTGCTCC ATCTCAGTTC
    AGTACATTAA AGATTTATTC TGACACTGTG GACTATGAAG ATGCATGTTT GATTGTTCGC
    TACTTGGCCT CTATGAGGCC GTTTGCCCAG AGCTTCGATA TTTATTTAAC ACAGATTCTG
    CGTGTACTTG GTGAAAATGC AATTGCAGTT CGAACAAAAG CCATGAAGTG TTTGTCTGAG
    GTTGTTGCTG TAGATCCCAG TATTCTAGCC AGGCCGGATA TGCAGCGAGG TGTTCATGGA
    CGGTTAATGG ATAACTCCAC CAGTGTACGG GAAGCAGCTG TGGAGCTGCT TGGCCGATTT
    GTGCTCTGTC GTCCTCAGCT TGCTGAGCAG TATTATGACA TGCTGATTGA AAGAATATTG
    GATACTGGTA TCAGTGTCAG AAAAAGAGTC ATAAAGATAC TTAGGGATAT CTGCATTGAA
    CAACCAACGT TTCCCAAAAT TACAGAGATG TGTGTGAAAA TGATTCGGAG AGTCAATGAT
    GAAGAAGGCA TTAAGAAATT AGTAAATGAG ACATTCCAGA AGCTGTGGTT TACTCCAACT
    CCACACCATG ACAAAGAAGC AATGACTAGG AAAATACTGA ACATCACTGA TGTGGTGGCA
    GCTTGTAGGG ATACAGGGTA TGATTGGTTT GAGCAGCTTC TTCAAAATCT ATTGAAGTCA
    GAAGAGGATG CTTCATATAA ACCTGTGAAG AAGGCTTGTA CTCAACTTGT TGACAATTTG
    GTTGAGCACA TCCTTAAATA TGAGGAGTCT TTATCAGACT CTGACAACAA AGGAGTGAAT
    TCTGGTCGCT TGGTTGCTTG TATAACCACT CTGTTCTTAT TCAGCAAAAT CAGGCCCCAG
    TTAATGGTCA AACATGCCAT GACCATGCAG CCATATCTGG CAACTAAATG TAGTACTCAG
    AATGATTTCA TGGTGATCTG CAATGTTGCA AAAATCTTGG AGCTTGTAGT GCCGTTAATG
    GAACACCCAA GTGAGACCTT CCTTGCCACT ATTGAGGAAG ACCTCATGAA ACTCATCATC
    AAATATGGCA TGACAGTTGT TCAACATTGT GTGAGCTGCC TTGGGTCTGT TGTGAACAAG
    GTCACCCAGA ATTACAAGTT TGTGTGGGCC TGTTTTAATA GATACTATGG TGCACTTTCA
    AAATTAAAAA GTCAACACCA AGAAGACCCC AACAGTACAA TACTTACTGC CAATAAACCA
    GCACTCCTTA GATCACTTTT CACTGTTGGA GCACTGTGTC GGCATTTTGA TTTTGACCAT
    GAAGATTTTA AGGGCAACAG TAAGGTTAAC ATTAAGGATA AAGTACTTGA ACTGCTGATG
    TATTTTACAA AGCATTCAGA CGAAGAAGTA CAGACAAAAG CCATTATTGG CCTGGGATTT
    GCATTTATAC AACACCCAAG CTTAATGTTT GAACAGGAAG TAAAGACTCT GTACAACAGC
    ATTCTTTCAG ATAAGAACTG TTCAGTAAAC CTAAAAATCC AGGTGTTAAA AAACCTCCAG
    ACCTACTTGC AGGAGGAGGA CACACGTATG CAGCAGGCAG ACAGAGACTG GAAAAAAGTA
    GCAAAACAGG AAGACCTGAA AGAAATGGGT GATATTTCCT CAGGGATGAG TAGTTCCATC
    ATGCAGCTGT ATCTCAAACA GGTCCTTGAG GCTTTCTTTC ATACCCAGTC CAATGTACGC
    CACTTTGCTC TAAATGTCAT TGCACTGACG TTAAACCAGG GTCTCATCCA TCCTGTTCAG
    TGTGTGCCAT ACTTAATTGC TATGGGCACA GATCCAGAGC CTTCTATGCG AAACAAAGCT
    GACCAGCAGT TGGTGGAAAT AGACAAAAAG TATGCTGGTT TCATTCACAT GAAAGCAGTG
    GCTGGTATGA AGATGTCTTA CCAGGTACAA CAGGCAATCA ATACCTGCCC AAAGGATCCT
    GTAAGAGGCT TTAGACACGA TGAATCATCC AGTGCATTGT GTTCACACCT ATATTCTATG
    ATCCGAGGGA ACCGTCAACA CAGACGAGCA TTTCTAATTT CTTTACTCAA TCTCTTCGAT
    GATACTGCAA AAACAGAGGT GAATATGCTC TTGTACATAG CAGACAATCT AGCCTGTTTT
    CCTTATCAAA CGCAGGAAGA GCCACTGTTT ATAATGCATC ATATAGACAT TACACTATCA
    GTTTCTGGTA GCAACCTACT ACAGTCATTT AAAGAGTCAA TGGTGAAAGA CAAAAAAAAG
    GAAAGAAAGC CTTCATCAGA TGAGGAGACT GAGAGTGACA GTAATAGTGG CAGTGAGAGC
    GAAAGTGAAG AGGAAGCTTC TAAGCCTCGG AGGTTACGAA AACGAGTAGA TTCTGACTCA
    GATTCTGATG AAGAAAATGA TATAAATGCT GTGATGAAAT GTTTACCAGA AAACTCAGCT
    CCTTTAATTG AATTTGCAAA TGTGTCTCAA GGCATATTAT TACTTTTGAT GCTGAAACAG
    CATTTAAAGA ACCTTTGCGG ATTCTCTGAT AGTAAAATTC AGAAATATTC TCCATCTGAG
    TCTGCAAAAG TTTATGATAA AGCGATAAAC AGGAAGACAG GAGTGCATTT CCATCCAAAA
    CAAACTCTGG ATTTTCTACG GAGTGATATG GCTAATTCCA AGATCACTGA AGAAGTGAAG
    AGGAGTATAG TAAAACAGTA CTTAGATTTC AAGCTCCTTA TGGAACATTT GGATCCTGAT
    GAAGAAGAAG AAGATGGAGA GGTGTCAGCT AGCACAAATG CTCGGAACAA AGCAATTACC
    TCGCTGCTTG GAGGAGGCAG CCCTAAAAAC AATGCAGCTG AGACAGAGGA CGATGAAAGT
    GATGGGGAGG ATAGAGGAGG AGGCACTTCA GGGTCATTGA GAAGGTCAAA ACGAAATTCA
    GACTCTACGG AGTTGGCTTC ACAGATGAAT GAAAGTGTTG ATGTCATGGA TGTCATCGCT
    ATTTGCTGTC CAAAGTACAA AGATCGACCA CAAATTGCAA GAGTAGTACA GAAAACCAGC
    AATGGCTTCA GTGTTCAGTG GATGGCAGGC TCCTACAGTG GCTCCTGGAC TGAGGCTAAG
    CGCCGTGATG GCCGCAAACT GGTGCCTTGG GTAGACACTA TTAAAGAGTC AGACATTATT
    TACAAAAAAA TTGCTCTAAC GAGTGCTAAT AAGCTGACTA ATAAAGTTGT TCAGACTTTA
    CGATCGCTGT ATGCCGCCAA GGATGGAACT TCGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133007.1
    CDS3247..10965
    Translation

    Target ORF information:

    RefSeq Version XM_015277521.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277521.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGA TAAGCCTCTG AAGAAGCGAA AACAAGAAAA CAAACCACAG
    GAGGCTGGGG GTGCTGCTGG AGGAAATAGG CCAGCTTCTC AGGAGTCAGG TTCTGCAGGA
    AATGGGGCAA GGCCAGCACT GATGGTTAGT ATTGATCTTC AACAAGCAGG AAGAGCGGAC
    TCTCAGGCAA CAATAACTCA GGAATTTGAG GCTATCAAAA AACCTGAAGA GATTAAGCAG
    TATAATGATG GATTTGTGTG TATTCCCCAA GAAGAGACTG TTGGAGTTCT TAAACTTAAA
    CCTGAAAACC ACCCTGAGAT TTTTAGGAAA AAGTCAGACT TTGAGGTTCA AAAGACAGAT
    ATCCAGCAAA ATGAAAACAG ACAAATGGAG TTTCAGCAAA ATGAGAGCAG AAGGTTGGAA
    AGTAAATACA ATGACAATAA ACAGGTAGAA GCTAAATATG ACATCAAGCA TGACAGTAGA
    CAAATGGAGA TGAAACAAAA TGAGAACAGA CAGGCAGAGT CAAAACAAAA TGAGAGCAGA
    CAAATGGAGA TGAAACAAAA TGAGGGAAAA CAAAATAATG GCAAACAAGA AATAAGACAG
    AGACCTGAAA CACCAAAGCA GAAGAGCGAA GGCAGGCCTG AAACACCCAA GCAGAAGAGT
    GAAGGCAGGC CTGAAACACC AAAACATAGG CATGATAACA GGAGGGACTC CAGTAAACCA
    TTGTCAGAAA AGAAAATTGA ATTATCTAGG CATAAACCTG ATGTGAAATC TGATACTTCA
    AAGGTAAAAT CTGATAGTAG ATCAGATCCC GTGAAGCAAA GGCCTGATGG GCGTTCAGTT
    TCTGATACTC AGAGGCGTGA CCACGATAGC CTTAAACAGA GATCTGAGGA CAGGGAATCA
    GAAAGATACA AAGGAGATCC ATCCAAGATT AGAAGGACTG AATTTTTGAG ATCCTCAAAC
    AGAAATGAAC ATGATTCTAA GTATGGTATT AAATCTGATG GTTCAAAACC TGAAAAATTT
    GAAAGGAAAC ACAGACATGA CTCTGGTGAA TCAAGGGAAA GATTTTCTTC TGGGGATCAG
    AAGTCAGATC AAAAGTCAAG ACCTGACAGC CCTCGAGTTA AACAGGATGG CAAAGGAGAT
    TCTAGCAAAT CAAGACCGGA TAAACCTGGT TTTAGATCAC CCAACAGCAA GGATGAACGA
    AGGACAGACA GTAGTAAGAG TAAAGTAGAG AGTAATAAAG CACATCCTGA CAATAAAGCA
    GAGTTTCCCA GTTATCTGCT GGGGGCAAGA TCTGGTGCAC TGAAACATTT TGTCATTCCA
    AAAATCAAAC GTGACAAAGA TGGCAATGTC ACTCAGGAGT CAAGGAAAAC TGAACTTAAA
    GGTGAACAGA AGGACAAAGT AGAGAAGATT GGGCTAGTTG AAGACCTAAA TAAAGGAGCT
    AAGCCTGTAG TTGTCCTTCA GAAGCTTTCT TTGGACGATG TACAAAAATT TATTAAGGAT
    AGAGAAGATA AATCAAGGGG CTCTTGTTTT AAACCTAACA AGAACAAGTC GTCCAAATCT
    AATAAAGGTA GCATAGATCA GTCAGTGCTA AAGGAGTTGC CTCCTGAGCT CCTGGCAGAA
    ATTGAATCCA CCATGCCTCT TTGTGAACGC GTGAAAATGA ACAAGCGCAA ACGTAGCACA
    GTTAATGAGA AACCAAAATA TGCTGAAATC AGTTCTGATG AAGACAATGA CAGTGAAGAA
    GCTTTTGAAT CTTCTCGAAA AAGACATAAA AAGGATAGAG ATGAAGCTTG GGAGTACGAA
    GAGCATGACA GAAGAAGTTC TGGTGACCAC AGGAGGAGTG GTCATTATCA TGAAGGAAGA
    AGGACTTCTG GTAGTGGCCG TTACCGCAAT CGCAGTCCCG ATGACTCTGA TCTGGATGAT
    TATTCTCCTC CTCCTAGCCT CAGTGAGGTG GCAAGAAAAA TGAAGAAAAA AGAAAAGCAA
    AAGAAGAGGA GAGCTTACGA GCCTAAACTA ACACCTGAAG AAATGATGGA TTCTTCAACC
    TTCAAAAGAT TTATTGCTTC AATAGAGAAT ATTCTGGAAA ATCTGGAAGA CATGGATTTT
    ACAACCTTGG GTGATGATGA TGAGATTCCA CAGGAATTGC TTTTGGGAAA ACATCAGCTT
    AATGAGTTGG GTAGTGAATC TGCAAAAATC AAGGCAATGG GTATTATGGA CAAGCTCTCA
    ACAGATAAAA TAGTAAAGGT CCTGAACATT TTGGAGAAGA ATATTCAAGA CGGATCAAAG
    CTTTCTACAT TACTTAACCA CAACAATGAC ACTGAAGATG AGGAGAGATT ATGGAGAGAT
    CTCATAATGG AGAGAGTAAC AAAATCTGCA GATGCTTGTC TTACTGCTAT CAATATTATG
    ACATCACCAA ATATGCCTAA AGCTGTTTAT ATTGAGGACG TAATAGAAAG AGTTATTCAG
    TACACAAAAT TTCATTTGCA GAACACTCTC TATCCTCAGT ATGACCCTGT GTATAGAGTG
    GATCCTCTTG GTGGTGGTCT TTTAAGTTCA AAAGCAAAAC GTGCCAAGTG TTCCACCCAC
    AAACAAAGAG TTATAGTGAT GTTGTATAAC AAAGTTTGTG ATATTGTCAG CAGTTTGTCA
    GAGTTGCTAG AGATACAACT TCTTACTGAT ACAACTATTC TTCAGGTATC GTCTATGGGG
    ATAACACCTT TCTTTGTAGA AAATGTCAGT GAACTACAGC TATGTGCCAT CAAATTAGTG
    ACTGCAGTCT TCTCCAGGTA TGAAAAACAT AGACAGCTAA TACTAGAAGA AATTTTTACT
    TCCCTTGCAA GACTACCAAC TAGCAAGAGG AGCCTGAGAA ACTTCAGGTT GAATAGCAGT
    GACACAGATG GAGAGCCTAT GTATATTCAG ATGGTAACAG CACTAGTTCT GCAGCTTATT
    CAATGTGTCG TGCACTTACC ATCAGCAGAA AAAGATTCTA ATTCAGATGA GGAATTGAAC
    AAAAAAGTGG ATCAAGATGT GCTTATTACT AACTCATATG AAACAGCTAT GAGAACAGCA
    CAAAATTTTC TTTCTATTTT TCTTAAGAAA TGTGGTAGTA AACAAGGAGA AGAAGATTAC
    AGGCCTCTCT TTGAGAACTT CATTCAAGAT CTTCTTTCCA CAGTCAATAA ACCAGAATGG
    CCAGCTGCAG AGTTGCTGCT TAGCTTACTA GGAAGGTTAT TGGTTCACCA GTTCAGTAAT
    AAATCAACAG AAATGGCTTT AAGAGTTGCG TCACTTGACT ATCTTGGAAC CGTAGCTGCA
    AGACTGAGGA AAGATGCAGT CACTAGTAAA ATGGATCAAG GATCTATAGC TAGAATCCTC
    AAACAGGCCT CAGGAGGAGA AGATGATATT CAACAGCTAC AGAAGGCTTT ATTGGATTAT
    CTGGATGAGA ACACTGAAAC TGATGCATCG TTAGTGTTTT CTCGGAAGTT TTATATAGCT
    CAGTGGTTTC GGGATACAAC TATGGAGACA GAGAAGGCAA TTAAATCTCA GAAGGATGAG
    GATTCATCTG AAGGAACTCA TCATGCAAAA GATGTTGAGA CCACAGGACA AATCATGCAC
    AGAGCAGAAA GCCGCAAGAA GTTTCTCCGC AGCATCATTA AAACTGCTCC ATCTCAGTTC
    AGTACATTAA AGATTTATTC TGACACTGTG GACTATGAAG ATGCATGTTT GATTGTTCGC
    TACTTGGCCT CTATGAGGCC GTTTGCCCAG AGCTTCGATA TTTATTTAAC ACAGATTCTG
    CGTGTACTTG GTGAAAATGC AATTGCAGTT CGAACAAAAG CCATGAAGTG TTTGTCTGAG
    GTTGTTGCTG TAGATCCCAG TATTCTAGCC AGGCCGGATA TGCAGCGAGG TGTTCATGGA
    CGGTTAATGG ATAACTCCAC CAGTGTACGG GAAGCAGCTG TGGAGCTGCT TGGCCGATTT
    GTGCTCTGTC GTCCTCAGCT TGCTGAGCAG TATTATGACA TGCTGATTGA AAGAATATTG
    GATACTGGTA TCAGTGTCAG AAAAAGAGTC ATAAAGATAC TTAGGGATAT CTGCATTGAA
    CAACCAACGT TTCCCAAAAT TACAGAGATG TGTGTGAAAA TGATTCGGAG AGTCAATGAT
    GAAGAAGGCA TTAAGAAATT AGTAAATGAG ACATTCCAGA AGCTGTGGTT TACTCCAACT
    CCACACCATG ACAAAGAAGC AATGACTAGG AAAATACTGA ACATCACTGA TGTGGTGGCA
    GCTTGTAGGG ATACAGGGTA TGATTGGTTT GAGCAGCTTC TTCAAAATCT ATTGAAGTCA
    GAAGAGGATG CTTCATATAA ACCTGTGAAG AAGGCTTGTA CTCAACTTGT TGACAATTTG
    GTTGAGCACA TCCTTAAATA TGAGGAGTCT TTATCAGACT CTGACAACAA AGGAGTGAAT
    TCTGGTCGCT TGGTTGCTTG TATAACCACT CTGTTCTTAT TCAGCAAAAT CAGGCCCCAG
    TTAATGGTCA AACATGCCAT GACCATGCAG CCATATCTGG CAACTAAATG TAGTACTCAG
    AATGATTTCA TGGTGATCTG CAATGTTGCA AAAATCTTGG AGCTTGTAGT GCCGTTAATG
    GAACACCCAA GTGAGACCTT CCTTGCCACT ATTGAGGAAG ACCTCATGAA ACTCATCATC
    AAATATGGCA TGACAGTTGT TCAACATTGT GTGAGCTGCC TTGGGTCTGT TGTGAACAAG
    GTCACCCAGA ATTACAAGTT TGTGTGGGCC TGTTTTAATA GATACTATGG TGCACTTTCA
    AAATTAAAAA GTCAACACCA AGAAGACCCC AACAGTACAA TACTTACTGC CAATAAACCA
    GCACTCCTTA GATCACTTTT CACTGTTGGA GCACTGTGTC GGCATTTTGA TTTTGACCAT
    GAAGATTTTA AGGGCAACAG TAAGGTTAAC ATTAAGGATA AAGTACTTGA ACTGCTGATG
    TATTTTACAA AGCATTCAGA CGAAGAAGTA CAGACAAAAG CCATTATTGG CCTGGGATTT
    GCATTTATAC AACACCCAAG CTTAATGTTT GAACAGGAAG TAAAGACTCT GTACAACAGC
    ATTCTTTCAG ATAAGAACTG TTCAGTAAAC CTAAAAATCC AGGTGTTAAA AAACCTCCAG
    ACCTACTTGC AGGAGGAGGA CACACGTATG CAGCAGGCAG ACAGAGACTG GAAAAAAGTA
    GCAAAACAGG AAGACCTGAA AGAAATGGGT GATATTTCCT CAGGGATGAG TAGTTCCATC
    ATGCAGCTGT ATCTCAAACA GGTCCTTGAG GCTTTCTTTC ATACCCAGTC CAATGTACGC
    CACTTTGCTC TAAATGTCAT TGCACTGACG TTAAACCAGG GTCTCATCCA TCCTGTTCAG
    TGTGTGCCAT ACTTAATTGC TATGGGCACA GATCCAGAGC CTTCTATGCG AAACAAAGCT
    GACCAGCAGT TGGTGGAAAT AGACAAAAAG TATGCTGGTT TCATTCACAT GAAAGCAGTG
    GCTGGTATGA AGATGTCTTA CCAGGTACAA CAGGCAATCA ATACCTGCCC AAAGGATCCT
    GTAAGAGGCT TTAGACACGA TGAATCATCC AGTGCATTGT GTTCACACCT ATATTCTATG
    ATCCGAGGGA ACCGTCAACA CAGACGAGCA TTTCTAATTT CTTTACTCAA TCTCTTCGAT
    GATACTGCAA AAACAGAGGT GAATATGCTC TTGTACATAG CAGACAATCT AGCCTGTTTT
    CCTTATCAAA CGCAGGAAGA GCCACTGTTT ATAATGCATC ATATAGACAT TACACTATCA
    GTTTCTGGTA GCAACCTACT ACAGTCATTT AAAGAGTCAA TGGTGAAAGA CAAAAAAAAG
    GAAAGAAAGC CTTCATCAGA TGAGGAGACT GAGAGTGACA GTAATAGTGG CAGTGAGAGC
    GAAAGTGAAG AGGAAGCTTC TAAGCCTCGG AGGTTACGAA AACGAGTAGA TTCTGACTCA
    GATTCTGATG AAGAAAATGA TATAAATGCT GTGATGAAAT GTTTACCAGA AAACTCAGCT
    CCTTTAATTG AATTTGCAAA TGTGTCTCAA GGCATATTAT TACTTTTGAT GCTGAAACAG
    CATTTAAAGA ACCTTTGCGG ATTCTCTGAT AGTAAAATTC AGAAATATTC TCCATCTGAG
    TCTGCAAAAG TTTATGATAA AGCGATAAAC AGGAAGACAG GAGTGCATTT CCATCCAAAA
    CAAACTCTGG ATTTTCTACG GAGTGATATG GCTAATTCCA AGATCACTGA AGAAGTGAAG
    AGGAGTATAG TAAAACAGTA CTTAGATTTC AAGCTCCTTA TGGAACATTT GGATCCTGAT
    GAAGAAGAAG AAGATGGAGA GGTGTCAGCT AGCACAAATG CTCGGAACAA AGCAATTACC
    TCGCTGCTTG GAGGAGGCAG CCCTAAAAAC AATGCAGCTG AGACAGAGGA CGATGAAAGT
    GATGGGGAGG ATAGAGGAGG AGGCACTTCA GGGTCATTGA GAAGGTCAAA ACGAAATTCA
    GACTCTACGG AGTTGGCTTC ACAGATGAAT GAAAGTGTTG ATGTCATGGA TGTCATCGCT
    ATTTGCTGTC CAAAGTACAA AGATCGACCA CAAATTGCAA GAGTAGTACA GAAAACCAGC
    AATGGCTTCA GTGTTCAGTG GATGGCAGGC TCCTACAGTG GCTCCTGGAC TGAGGCTAAG
    CGCCGTGATG GCCGCAAACT GGTGCCTTGG GTAGACACTA TTAAAGAGTC AGACATTATT
    TACAAAAAAA TTGCTCTAAC GAGTGCTAAT AAGCTGACTA ATAAAGTTGT TCAGACTTTA
    CGATCGCTGT ATGCCGCCAA GGATGGAACT TCGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa20948
    Clone ID Related Accession (Same CDS sequence) XM_015277521.1 , XM_015277521.2
    Accession Version XM_015277521.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7719bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product nipped-B-like protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277521.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGA TAAGCCTCTG AAGAAGCGAA AACAAGAAAA CAAACCACAG
    GAGGCTGGGG GTGCTGCTGG AGGAAATAGG CCAGCTTCTC AGGAGTCAGG TTCTGCAGGA
    AATGGGGCAA GGCCAGCACT GATGGTTAGT ATTGATCTTC AACAAGCAGG AAGAGCGGAC
    TCTCAGGCAA CAATAACTCA GGAATTTGAG GCTATCAAAA AACCTGAAGA GATTAAGCAG
    TATAATGATG GATTTGTGTG TATTCCCCAA GAAGAGACTG TTGGAGTTCT TAAACTTAAA
    CCTGAAAACC ACCCTGAGAT TTTTAGGAAA AAGTCAGACT TTGAGGTTCA AAAGACAGAT
    ATCCAGCAAA ATGAAAACAG ACAAATGGAG TTTCAGCAAA ATGAGAGCAG AAGGTTGGAA
    AGTAAATACA ATGACAATAA ACAGGTAGAA GCTAAATATG ACATCAAGCA TGACAGTAGA
    CAAATGGAGA TGAAACAAAA TGAGAACAGA CAGGCAGAGT CAAAACAAAA TGAGAGCAGA
    CAAATGGAGA TGAAACAAAA TGAGGGAAAA CAAAATAATG GCAAACAAGA AATAAGACAG
    AGACCTGAAA CACCAAAGCA GAAGAGCGAA GGCAGGCCTG AAACACCCAA GCAGAAGAGT
    GAAGGCAGGC CTGAAACACC AAAACATAGG CATGATAACA GGAGGGACTC CAGTAAACCA
    TTGTCAGAAA AGAAAATTGA ATTATCTAGG CATAAACCTG ATGTGAAATC TGATACTTCA
    AAGGTAAAAT CTGATAGTAG ATCAGATCCC GTGAAGCAAA GGCCTGATGG GCGTTCAGTT
    TCTGATACTC AGAGGCGTGA CCACGATAGC CTTAAACAGA GATCTGAGGA CAGGGAATCA
    GAAAGATACA AAGGAGATCC ATCCAAGATT AGAAGGACTG AATTTTTGAG ATCCTCAAAC
    AGAAATGAAC ATGATTCTAA GTATGGTATT AAATCTGATG GTTCAAAACC TGAAAAATTT
    GAAAGGAAAC ACAGACATGA CTCTGGTGAA TCAAGGGAAA GATTTTCTTC TGGGGATCAG
    AAGTCAGATC AAAAGTCAAG ACCTGACAGC CCTCGAGTTA AACAGGATGG CAAAGGAGAT
    TCTAGCAAAT CAAGACCGGA TAAACCTGGT TTTAGATCAC CCAACAGCAA GGATGAACGA
    AGGACAGACA GTAGTAAGAG TAAAGTAGAG AGTAATAAAG CACATCCTGA CAATAAAGCA
    GAGTTTCCCA GTTATCTGCT GGGGGCAAGA TCTGGTGCAC TGAAACATTT TGTCATTCCA
    AAAATCAAAC GTGACAAAGA TGGCAATGTC ACTCAGGAGT CAAGGAAAAC TGAACTTAAA
    GGTGAACAGA AGGACAAAGT AGAGAAGATT GGGCTAGTTG AAGACCTAAA TAAAGGAGCT
    AAGCCTGTAG TTGTCCTTCA GAAGCTTTCT TTGGACGATG TACAAAAATT TATTAAGGAT
    AGAGAAGATA AATCAAGGGG CTCTTGTTTT AAACCTAACA AGAACAAGTC GTCCAAATCT
    AATAAAGGTA GCATAGATCA GTCAGTGCTA AAGGAGTTGC CTCCTGAGCT CCTGGCAGAA
    ATTGAATCCA CCATGCCTCT TTGTGAACGC GTGAAAATGA ACAAGCGCAA ACGTAGCACA
    GTTAATGAGA AACCAAAATA TGCTGAAATC AGTTCTGATG AAGACAATGA CAGTGAAGAA
    GCTTTTGAAT CTTCTCGAAA AAGACATAAA AAGGATAGAG ATGAAGCTTG GGAGTACGAA
    GAGCATGACA GAAGAAGTTC TGGTGACCAC AGGAGGAGTG GTCATTATCA TGAAGGAAGA
    AGGACTTCTG GTAGTGGCCG TTACCGCAAT CGCAGTCCCG ATGACTCTGA TCTGGATGAT
    TATTCTCCTC CTCCTAGCCT CAGTGAGGTG GCAAGAAAAA TGAAGAAAAA AGAAAAGCAA
    AAGAAGAGGA GAGCTTACGA GCCTAAACTA ACACCTGAAG AAATGATGGA TTCTTCAACC
    TTCAAAAGAT TTATTGCTTC AATAGAGAAT ATTCTGGAAA ATCTGGAAGA CATGGATTTT
    ACAACCTTGG GTGATGATGA TGAGATTCCA CAGGAATTGC TTTTGGGAAA ACATCAGCTT
    AATGAGTTGG GTAGTGAATC TGCAAAAATC AAGGCAATGG GTATTATGGA CAAGCTCTCA
    ACAGATAAAA TAGTAAAGGT CCTGAACATT TTGGAGAAGA ATATTCAAGA CGGATCAAAG
    CTTTCTACAT TACTTAACCA CAACAATGAC ACTGAAGATG AGGAGAGATT ATGGAGAGAT
    CTCATAATGG AGAGAGTAAC AAAATCTGCA GATGCTTGTC TTACTGCTAT CAATATTATG
    ACATCACCAA ATATGCCTAA AGCTGTTTAT ATTGAGGACG TAATAGAAAG AGTTATTCAG
    TACACAAAAT TTCATTTGCA GAACACTCTC TATCCTCAGT ATGACCCTGT GTATAGAGTG
    GATCCTCTTG GTGGTGGTCT TTTAAGTTCA AAAGCAAAAC GTGCCAAGTG TTCCACCCAC
    AAACAAAGAG TTATAGTGAT GTTGTATAAC AAAGTTTGTG ATATTGTCAG CAGTTTGTCA
    GAGTTGCTAG AGATACAACT TCTTACTGAT ACAACTATTC TTCAGGTATC GTCTATGGGG
    ATAACACCTT TCTTTGTAGA AAATGTCAGT GAACTACAGC TATGTGCCAT CAAATTAGTG
    ACTGCAGTCT TCTCCAGGTA TGAAAAACAT AGACAGCTAA TACTAGAAGA AATTTTTACT
    TCCCTTGCAA GACTACCAAC TAGCAAGAGG AGCCTGAGAA ACTTCAGGTT GAATAGCAGT
    GACACAGATG GAGAGCCTAT GTATATTCAG ATGGTAACAG CACTAGTTCT GCAGCTTATT
    CAATGTGTCG TGCACTTACC ATCAGCAGAA AAAGATTCTA ATTCAGATGA GGAATTGAAC
    AAAAAAGTGG ATCAAGATGT GCTTATTACT AACTCATATG AAACAGCTAT GAGAACAGCA
    CAAAATTTTC TTTCTATTTT TCTTAAGAAA TGTGGTAGTA AACAAGGAGA AGAAGATTAC
    AGGCCTCTCT TTGAGAACTT CATTCAAGAT CTTCTTTCCA CAGTCAATAA ACCAGAATGG
    CCAGCTGCAG AGTTGCTGCT TAGCTTACTA GGAAGGTTAT TGGTTCACCA GTTCAGTAAT
    AAATCAACAG AAATGGCTTT AAGAGTTGCG TCACTTGACT ATCTTGGAAC CGTAGCTGCA
    AGACTGAGGA AAGATGCAGT CACTAGTAAA ATGGATCAAG GATCTATAGC TAGAATCCTC
    AAACAGGCCT CAGGAGGAGA AGATGATATT CAACAGCTAC AGAAGGCTTT ATTGGATTAT
    CTGGATGAGA ACACTGAAAC TGATGCATCG TTAGTGTTTT CTCGGAAGTT TTATATAGCT
    CAGTGGTTTC GGGATACAAC TATGGAGACA GAGAAGGCAA TTAAATCTCA GAAGGATGAG
    GATTCATCTG AAGGAACTCA TCATGCAAAA GATGTTGAGA CCACAGGACA AATCATGCAC
    AGAGCAGAAA GCCGCAAGAA GTTTCTCCGC AGCATCATTA AAACTGCTCC ATCTCAGTTC
    AGTACATTAA AGATTTATTC TGACACTGTG GACTATGAAG ATGCATGTTT GATTGTTCGC
    TACTTGGCCT CTATGAGGCC GTTTGCCCAG AGCTTCGATA TTTATTTAAC ACAGATTCTG
    CGTGTACTTG GTGAAAATGC AATTGCAGTT CGAACAAAAG CCATGAAGTG TTTGTCTGAG
    GTTGTTGCTG TAGATCCCAG TATTCTAGCC AGGCCGGATA TGCAGCGAGG TGTTCATGGA
    CGGTTAATGG ATAACTCCAC CAGTGTACGG GAAGCAGCTG TGGAGCTGCT TGGCCGATTT
    GTGCTCTGTC GTCCTCAGCT TGCTGAGCAG TATTATGACA TGCTGATTGA AAGAATATTG
    GATACTGGTA TCAGTGTCAG AAAAAGAGTC ATAAAGATAC TTAGGGATAT CTGCATTGAA
    CAACCAACGT TTCCCAAAAT TACAGAGATG TGTGTGAAAA TGATTCGGAG AGTCAATGAT
    GAAGAAGGCA TTAAGAAATT AGTAAATGAG ACATTCCAGA AGCTGTGGTT TACTCCAACT
    CCACACCATG ACAAAGAAGC AATGACTAGG AAAATACTGA ACATCACTGA TGTGGTGGCA
    GCTTGTAGGG ATACAGGGTA TGATTGGTTT GAGCAGCTTC TTCAAAATCT ATTGAAGTCA
    GAAGAGGATG CTTCATATAA ACCTGTGAAG AAGGCTTGTA CTCAACTTGT TGACAATTTG
    GTTGAGCACA TCCTTAAATA TGAGGAGTCT TTATCAGACT CTGACAACAA AGGAGTGAAT
    TCTGGTCGCT TGGTTGCTTG TATAACCACT CTGTTCTTAT TCAGCAAAAT CAGGCCCCAG
    TTAATGGTCA AACATGCCAT GACCATGCAG CCATATCTGG CAACTAAATG TAGTACTCAG
    AATGATTTCA TGGTGATCTG CAATGTTGCA AAAATCTTGG AGCTTGTAGT GCCGTTAATG
    GAACACCCAA GTGAGACCTT CCTTGCCACT ATTGAGGAAG ACCTCATGAA ACTCATCATC
    AAATATGGCA TGACAGTTGT TCAACATTGT GTGAGCTGCC TTGGGTCTGT TGTGAACAAG
    GTCACCCAGA ATTACAAGTT TGTGTGGGCC TGTTTTAATA GATACTATGG TGCACTTTCA
    AAATTAAAAA GTCAACACCA AGAAGACCCC AACAGTACAA TACTTACTGC CAATAAACCA
    GCACTCCTTA GATCACTTTT CACTGTTGGA GCACTGTGTC GGCATTTTGA TTTTGACCAT
    GAAGATTTTA AGGGCAACAG TAAGGTTAAC ATTAAGGATA AAGTACTTGA ACTGCTGATG
    TATTTTACAA AGCATTCAGA CGAAGAAGTA CAGACAAAAG CCATTATTGG CCTGGGATTT
    GCATTTATAC AACACCCAAG CTTAATGTTT GAACAGGAAG TAAAGACTCT GTACAACAGC
    ATTCTTTCAG ATAAGAACTG TTCAGTAAAC CTAAAAATCC AGGTGTTAAA AAACCTCCAG
    ACCTACTTGC AGGAGGAGGA CACACGTATG CAGCAGGCAG ACAGAGACTG GAAAAAAGTA
    GCAAAACAGG AAGACCTGAA AGAAATGGGT GATATTTCCT CAGGGATGAG TAGTTCCATC
    ATGCAGCTGT ATCTCAAACA GGTCCTTGAG GCTTTCTTTC ATACCCAGTC CAATGTACGC
    CACTTTGCTC TAAATGTCAT TGCACTGACG TTAAACCAGG GTCTCATCCA TCCTGTTCAG
    TGTGTGCCAT ACTTAATTGC TATGGGCACA GATCCAGAGC CTTCTATGCG AAACAAAGCT
    GACCAGCAGT TGGTGGAAAT AGACAAAAAG TATGCTGGTT TCATTCACAT GAAAGCAGTG
    GCTGGTATGA AGATGTCTTA CCAGGTACAA CAGGCAATCA ATACCTGCCC AAAGGATCCT
    GTAAGAGGCT TTAGACACGA TGAATCATCC AGTGCATTGT GTTCACACCT ATATTCTATG
    ATCCGAGGGA ACCGTCAACA CAGACGAGCA TTTCTAATTT CTTTACTCAA TCTCTTCGAT
    GATACTGCAA AAACAGAGGT GAATATGCTC TTGTACATAG CAGACAATCT AGCCTGTTTT
    CCTTATCAAA CGCAGGAAGA GCCACTGTTT ATAATGCATC ATATAGACAT TACACTATCA
    GTTTCTGGTA GCAACCTACT ACAGTCATTT AAAGAGTCAA TGGTGAAAGA CAAAAAAAAG
    GAAAGAAAGC CTTCATCAGA TGAGGAGACT GAGAGTGACA GTAATAGTGG CAGTGAGAGC
    GAAAGTGAAG AGGAAGCTTC TAAGCCTCGG AGGTTACGAA AACGAGTAGA TTCTGACTCA
    GATTCTGATG AAGAAAATGA TATAAATGCT GTGATGAAAT GTTTACCAGA AAACTCAGCT
    CCTTTAATTG AATTTGCAAA TGTGTCTCAA GGCATATTAT TACTTTTGAT GCTGAAACAG
    CATTTAAAGA ACCTTTGCGG ATTCTCTGAT AGTAAAATTC AGAAATATTC TCCATCTGAG
    TCTGCAAAAG TTTATGATAA AGCGATAAAC AGGAAGACAG GAGTGCATTT CCATCCAAAA
    CAAACTCTGG ATTTTCTACG GAGTGATATG GCTAATTCCA AGATCACTGA AGAAGTGAAG
    AGGAGTATAG TAAAACAGTA CTTAGATTTC AAGCTCCTTA TGGAACATTT GGATCCTGAT
    GAAGAAGAAG AAGATGGAGA GGTGTCAGCT AGCACAAATG CTCGGAACAA AGCAATTACC
    TCGCTGCTTG GAGGAGGCAG CCCTAAAAAC AATGCAGCTG AGACAGAGGA CGATGAAAGT
    GATGGGGAGG ATAGAGGAGG AGGCACTTCA GGGTCATTGA GAAGGTCAAA ACGAAATTCA
    GACTCTACGG AGTTGGCTTC ACAGATGAAT GAAAGTGTTG ATGTCATGGA TGTCATCGCT
    ATTTGCTGTC CAAAGTACAA AGATCGACCA CAAATTGCAA GAGTAGTACA GAAAACCAGC
    AATGGCTTCA GTGTTCAGTG GATGGCAGGC TCCTACAGTG GCTCCTGGAC TGAGGCTAAG
    CGCCGTGATG GCCGCAAACT GGTGCCTTGG GTAGACACTA TTAAAGAGTC AGACATTATT
    TACAAAAAAA TTGCTCTAAC GAGTGCTAAT AAGCTGACTA ATAAAGTTGT TCAGACTTTA
    CGATCGCTGT ATGCCGCCAA GGATGGAACT TCGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133007.1
    CDS3239..10957
    Translation

    Target ORF information:

    RefSeq Version XM_015277521.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Nipped-B homolog-like (LOC427439), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277521.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CAGGGATTGC TAGTCTTACA 
    GACCTTTTGA ACCAGCTGCC TCTTCCATCA CCTTTACCTG CTACAACAAC AAAAAGCCTG
    CTCTTCAATG GACGAATAGC TGAAGAAGTG AACTGCCTTC TGGCTTGCAG GGATGAAAAT
    CTCGTTTCAC AGCTCATCCA CAGTCTCAAT CAGGTGTCAA CAGATCACAT AGAATTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAGGGA GATACACCAG TTTTGCTGCA AGCTATCCTG
    GCGAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAAA ACAGATATGG GGTACAAAGC
    GGGATGATGA TTCCACAATT TAATATTTCT CAGAATTCTA TGCGTGGTAG TCCTGCATCT
    TCCAATTATC AACAAACCAC TATTTCACAT AGCCCTTCCA GCCGGTTTGT GCCACCACAG
    ACAAGCTCTG GTAACAGATT TTTGGCACAA CAGAACAGTC CAGTGCCTAG TCCATATGCT
    CCTCAAAGCC CTGCAGGATA CATGCCATAT TCACATCCTC CAAGTTACAC ACCGCATCCT
    CAGCTGCAGC AAGCTTGTCC CATTGTCACA GCTGGGATGA GGAATCTCCA TGAAAATAAA
    GTTTCAAGTC AGTTGTCTGG AAATTCAGCT AATCATCATG CTGATAATTC TAGACATGGA
    TCAAATGAAG ACTACCTACA GATGGTGCAC AGATTAAGTA GCGATGATGG CGATCCTTCA
    ATAAGAAATG CTGCATCTTT TTCCTTGAGG TCACCACAGT CAGTCTGTTC TCCAGCTGGA
    AGTGATGGAA CCCCTAAAGA TAAGCCTCTG AAGAAGCGAA AACAAGAAAA CAAACCACAG
    GAGGCTGGGG GTGCTGCTGG AGGAAATAGG CCAGCTTCTC AGGAGTCAGG TTCTGCAGGA
    AATGGGGCAA GGCCAGCACT GATGGTTAGT ATTGATCTTC AACAAGCAGG AAGAGCGGAC
    TCTCAGGCAA CAATAACTCA GGAATTTGAG GCTATCAAAA AACCTGAAGA GATTAAGCAG
    TATAATGATG GATTTGTGTG TATTCCCCAA GAAGAGACTG TTGGAGTTCT TAAACTTAAA
    CCTGAAAACC ACCCTGAGAT TTTTAGGAAA AAGTCAGACT TTGAGGTTCA AAAGACAGAT
    ATCCAGCAAA ATGAAAACAG ACAAATGGAG TTTCAGCAAA ATGAGAGCAG AAGGTTGGAA
    AGTAAATACA ATGACAATAA ACAGGTAGAA GCTAAATATG ACATCAAGCA TGACAGTAGA
    CAAATGGAGA TGAAACAAAA TGAGAACAGA CAGGCAGAGT CAAAACAAAA TGAGAGCAGA
    CAAATGGAGA TGAAACAAAA TGAGGGAAAA CAAAATAATG GCAAACAAGA AATAAGACAG
    AGACCTGAAA CACCAAAGCA GAAGAGCGAA GGCAGGCCTG AAACACCCAA GCAGAAGAGT
    GAAGGCAGGC CTGAAACACC AAAACATAGG CATGATAACA GGAGGGACTC CAGTAAACCA
    TTGTCAGAAA AGAAAATTGA ATTATCTAGG CATAAACCTG ATGTGAAATC TGATACTTCA
    AAGGTAAAAT CTGATAGTAG ATCAGATCCC GTGAAGCAAA GGCCTGATGG GCGTTCAGTT
    TCTGATACTC AGAGGCGTGA CCACGATAGC CTTAAACAGA GATCTGAGGA CAGGGAATCA
    GAAAGATACA AAGGAGATCC ATCCAAGATT AGAAGGACTG AATTTTTGAG ATCCTCAAAC
    AGAAATGAAC ATGATTCTAA GTATGGTATT AAATCTGATG GTTCAAAACC TGAAAAATTT
    GAAAGGAAAC ACAGACATGA CTCTGGTGAA TCAAGGGAAA GATTTTCTTC TGGGGATCAG
    AAGTCAGATC AAAAGTCAAG ACCTGACAGC CCTCGAGTTA AACAGGATGG CAAAGGAGAT
    TCTAGCAAAT CAAGACCGGA TAAACCTGGT TTTAGATCAC CCAACAGCAA GGATGAACGA
    AGGACAGACA GTAGTAAGAG TAAAGTAGAG AGTAATAAAG CACATCCTGA CAATAAAGCA
    GAGTTTCCCA GTTATCTGCT GGGGGCAAGA TCTGGTGCAC TGAAACATTT TGTCATTCCA
    AAAATCAAAC GTGACAAAGA TGGCAATGTC ACTCAGGAGT CAAGGAAAAC TGAACTTAAA
    GGTGAACAGA AGGACAAAGT AGAGAAGATT GGGCTAGTTG AAGACCTAAA TAAAGGAGCT
    AAGCCTGTAG TTGTCCTTCA GAAGCTTTCT TTGGACGATG TACAAAAATT TATTAAGGAT
    AGAGAAGATA AATCAAGGGG CTCTTGTTTT AAACCTAACA AGAACAAGTC GTCCAAATCT
    AATAAAGGTA GCATAGATCA GTCAGTGCTA AAGGAGTTGC CTCCTGAGCT CCTGGCAGAA
    ATTGAATCCA CCATGCCTCT TTGTGAACGC GTGAAAATGA ACAAGCGCAA ACGTAGCACA
    GTTAATGAGA AACCAAAATA TGCTGAAATC AGTTCTGATG AAGACAATGA CAGTGAAGAA
    GCTTTTGAAT CTTCTCGAAA AAGACATAAA AAGGATAGAG ATGAAGCTTG GGAGTACGAA
    GAGCATGACA GAAGAAGTTC TGGTGACCAC AGGAGGAGTG GTCATTATCA TGAAGGAAGA
    AGGACTTCTG GTAGTGGCCG TTACCGCAAT CGCAGTCCCG ATGACTCTGA TCTGGATGAT
    TATTCTCCTC CTCCTAGCCT CAGTGAGGTG GCAAGAAAAA TGAAGAAAAA AGAAAAGCAA
    AAGAAGAGGA GAGCTTACGA GCCTAAACTA ACACCTGAAG AAATGATGGA TTCTTCAACC
    TTCAAAAGAT TTATTGCTTC AATAGAGAAT ATTCTGGAAA ATCTGGAAGA CATGGATTTT
    ACAACCTTGG GTGATGATGA TGAGATTCCA CAGGAATTGC TTTTGGGAAA ACATCAGCTT
    AATGAGTTGG GTAGTGAATC TGCAAAAATC AAGGCAATGG GTATTATGGA CAAGCTCTCA
    ACAGATAAAA TAGTAAAGGT CCTGAACATT TTGGAGAAGA ATATTCAAGA CGGATCAAAG
    CTTTCTACAT TACTTAACCA CAACAATGAC ACTGAAGATG AGGAGAGATT ATGGAGAGAT
    CTCATAATGG AGAGAGTAAC AAAATCTGCA GATGCTTGTC TTACTGCTAT CAATATTATG
    ACATCACCAA ATATGCCTAA AGCTGTTTAT ATTGAGGACG TAATAGAAAG AGTTATTCAG
    TACACAAAAT TTCATTTGCA GAACACTCTC TATCCTCAGT ATGACCCTGT GTATAGAGTG
    GATCCTCTTG GTGGTGGTCT TTTAAGTTCA AAAGCAAAAC GTGCCAAGTG TTCCACCCAC
    AAACAAAGAG TTATAGTGAT GTTGTATAAC AAAGTTTGTG ATATTGTCAG CAGTTTGTCA
    GAGTTGCTAG AGATACAACT TCTTACTGAT ACAACTATTC TTCAGGTATC GTCTATGGGG
    ATAACACCTT TCTTTGTAGA AAATGTCAGT GAACTACAGC TATGTGCCAT CAAATTAGTG
    ACTGCAGTCT TCTCCAGGTA TGAAAAACAT AGACAGCTAA TACTAGAAGA AATTTTTACT
    TCCCTTGCAA GACTACCAAC TAGCAAGAGG AGCCTGAGAA ACTTCAGGTT GAATAGCAGT
    GACACAGATG GAGAGCCTAT GTATATTCAG ATGGTAACAG CACTAGTTCT GCAGCTTATT
    CAATGTGTCG TGCACTTACC ATCAGCAGAA AAAGATTCTA ATTCAGATGA GGAATTGAAC
    AAAAAAGTGG ATCAAGATGT GCTTATTACT AACTCATATG AAACAGCTAT GAGAACAGCA
    CAAAATTTTC TTTCTATTTT TCTTAAGAAA TGTGGTAGTA AACAAGGAGA AGAAGATTAC
    AGGCCTCTCT TTGAGAACTT CATTCAAGAT CTTCTTTCCA CAGTCAATAA ACCAGAATGG
    CCAGCTGCAG AGTTGCTGCT TAGCTTACTA GGAAGGTTAT TGGTTCACCA GTTCAGTAAT
    AAATCAACAG AAATGGCTTT AAGAGTTGCG TCACTTGACT ATCTTGGAAC CGTAGCTGCA
    AGACTGAGGA AAGATGCAGT CACTAGTAAA ATGGATCAAG GATCTATAGC TAGAATCCTC
    AAACAGGCCT CAGGAGGAGA AGATGATATT CAACAGCTAC AGAAGGCTTT ATTGGATTAT
    CTGGATGAGA ACACTGAAAC TGATGCATCG TTAGTGTTTT CTCGGAAGTT TTATATAGCT
    CAGTGGTTTC GGGATACAAC TATGGAGACA GAGAAGGCAA TTAAATCTCA GAAGGATGAG
    GATTCATCTG AAGGAACTCA TCATGCAAAA GATGTTGAGA CCACAGGACA AATCATGCAC
    AGAGCAGAAA GCCGCAAGAA GTTTCTCCGC AGCATCATTA AAACTGCTCC ATCTCAGTTC
    AGTACATTAA AGATTTATTC TGACACTGTG GACTATGAAG ATGCATGTTT GATTGTTCGC
    TACTTGGCCT CTATGAGGCC GTTTGCCCAG AGCTTCGATA TTTATTTAAC ACAGATTCTG
    CGTGTACTTG GTGAAAATGC AATTGCAGTT CGAACAAAAG CCATGAAGTG TTTGTCTGAG
    GTTGTTGCTG TAGATCCCAG TATTCTAGCC AGGCCGGATA TGCAGCGAGG TGTTCATGGA
    CGGTTAATGG ATAACTCCAC CAGTGTACGG GAAGCAGCTG TGGAGCTGCT TGGCCGATTT
    GTGCTCTGTC GTCCTCAGCT TGCTGAGCAG TATTATGACA TGCTGATTGA AAGAATATTG
    GATACTGGTA TCAGTGTCAG AAAAAGAGTC ATAAAGATAC TTAGGGATAT CTGCATTGAA
    CAACCAACGT TTCCCAAAAT TACAGAGATG TGTGTGAAAA TGATTCGGAG AGTCAATGAT
    GAAGAAGGCA TTAAGAAATT AGTAAATGAG ACATTCCAGA AGCTGTGGTT TACTCCAACT
    CCACACCATG ACAAAGAAGC AATGACTAGG AAAATACTGA ACATCACTGA TGTGGTGGCA
    GCTTGTAGGG ATACAGGGTA TGATTGGTTT GAGCAGCTTC TTCAAAATCT ATTGAAGTCA
    GAAGAGGATG CTTCATATAA ACCTGTGAAG AAGGCTTGTA CTCAACTTGT TGACAATTTG
    GTTGAGCACA TCCTTAAATA TGAGGAGTCT TTATCAGACT CTGACAACAA AGGAGTGAAT
    TCTGGTCGCT TGGTTGCTTG TATAACCACT CTGTTCTTAT TCAGCAAAAT CAGGCCCCAG
    TTAATGGTCA AACATGCCAT GACCATGCAG CCATATCTGG CAACTAAATG TAGTACTCAG
    AATGATTTCA TGGTGATCTG CAATGTTGCA AAAATCTTGG AGCTTGTAGT GCCGTTAATG
    GAACACCCAA GTGAGACCTT CCTTGCCACT ATTGAGGAAG ACCTCATGAA ACTCATCATC
    AAATATGGCA TGACAGTTGT TCAACATTGT GTGAGCTGCC TTGGGTCTGT TGTGAACAAG
    GTCACCCAGA ATTACAAGTT TGTGTGGGCC TGTTTTAATA GATACTATGG TGCACTTTCA
    AAATTAAAAA GTCAACACCA AGAAGACCCC AACAGTACAA TACTTACTGC CAATAAACCA
    GCACTCCTTA GATCACTTTT CACTGTTGGA GCACTGTGTC GGCATTTTGA TTTTGACCAT
    GAAGATTTTA AGGGCAACAG TAAGGTTAAC ATTAAGGATA AAGTACTTGA ACTGCTGATG
    TATTTTACAA AGCATTCAGA CGAAGAAGTA CAGACAAAAG CCATTATTGG CCTGGGATTT
    GCATTTATAC AACACCCAAG CTTAATGTTT GAACAGGAAG TAAAGACTCT GTACAACAGC
    ATTCTTTCAG ATAAGAACTG TTCAGTAAAC CTAAAAATCC AGGTGTTAAA AAACCTCCAG
    ACCTACTTGC AGGAGGAGGA CACACGTATG CAGCAGGCAG ACAGAGACTG GAAAAAAGTA
    GCAAAACAGG AAGACCTGAA AGAAATGGGT GATATTTCCT CAGGGATGAG TAGTTCCATC
    ATGCAGCTGT ATCTCAAACA GGTCCTTGAG GCTTTCTTTC ATACCCAGTC CAATGTACGC
    CACTTTGCTC TAAATGTCAT TGCACTGACG TTAAACCAGG GTCTCATCCA TCCTGTTCAG
    TGTGTGCCAT ACTTAATTGC TATGGGCACA GATCCAGAGC CTTCTATGCG AAACAAAGCT
    GACCAGCAGT TGGTGGAAAT AGACAAAAAG TATGCTGGTT TCATTCACAT GAAAGCAGTG
    GCTGGTATGA AGATGTCTTA CCAGGTACAA CAGGCAATCA ATACCTGCCC AAAGGATCCT
    GTAAGAGGCT TTAGACACGA TGAATCATCC AGTGCATTGT GTTCACACCT ATATTCTATG
    ATCCGAGGGA ACCGTCAACA CAGACGAGCA TTTCTAATTT CTTTACTCAA TCTCTTCGAT
    GATACTGCAA AAACAGAGGT GAATATGCTC TTGTACATAG CAGACAATCT AGCCTGTTTT
    CCTTATCAAA CGCAGGAAGA GCCACTGTTT ATAATGCATC ATATAGACAT TACACTATCA
    GTTTCTGGTA GCAACCTACT ACAGTCATTT AAAGAGTCAA TGGTGAAAGA CAAAAAAAAG
    GAAAGAAAGC CTTCATCAGA TGAGGAGACT GAGAGTGACA GTAATAGTGG CAGTGAGAGC
    GAAAGTGAAG AGGAAGCTTC TAAGCCTCGG AGGTTACGAA AACGAGTAGA TTCTGACTCA
    GATTCTGATG AAGAAAATGA TATAAATGCT GTGATGAAAT GTTTACCAGA AAACTCAGCT
    CCTTTAATTG AATTTGCAAA TGTGTCTCAA GGCATATTAT TACTTTTGAT GCTGAAACAG
    CATTTAAAGA ACCTTTGCGG ATTCTCTGAT AGTAAAATTC AGAAATATTC TCCATCTGAG
    TCTGCAAAAG TTTATGATAA AGCGATAAAC AGGAAGACAG GAGTGCATTT CCATCCAAAA
    CAAACTCTGG ATTTTCTACG GAGTGATATG GCTAATTCCA AGATCACTGA AGAAGTGAAG
    AGGAGTATAG TAAAACAGTA CTTAGATTTC AAGCTCCTTA TGGAACATTT GGATCCTGAT
    GAAGAAGAAG AAGATGGAGA GGTGTCAGCT AGCACAAATG CTCGGAACAA AGCAATTACC
    TCGCTGCTTG GAGGAGGCAG CCCTAAAAAC AATGCAGCTG AGACAGAGGA CGATGAAAGT
    GATGGGGAGG ATAGAGGAGG AGGCACTTCA GGGTCATTGA GAAGGTCAAA ACGAAATTCA
    GACTCTACGG AGTTGGCTTC ACAGATGAAT GAAAGTGTTG ATGTCATGGA TGTCATCGCT
    ATTTGCTGTC CAAAGTACAA AGATCGACCA CAAATTGCAA GAGTAGTACA GAAAACCAGC
    AATGGCTTCA GTGTTCAGTG GATGGCAGGC TCCTACAGTG GCTCCTGGAC TGAGGCTAAG
    CGCCGTGATG GCCGCAAACT GGTGCCTTGG GTAGACACTA TTAAAGAGTC AGACATTATT
    TACAAAAAAA TTGCTCTAAC GAGTGCTAAT AAGCTGACTA ATAAAGTTGT TCAGACTTTA
    CGATCGCTGT ATGCCGCCAA GGATGGAACT TCGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.