HSPA5 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSPA5
Entrez Gene ID	396487
Full Name	heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Synonyms	GRP78
General protein information	Preferred Names heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) Names 78 kDa glucose-regulated protein GRP-78 biP heat shock 70 kDa protein 5 immunoglobulin heavy chain-binding protein
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205491.1	NP_990822.1	endoplasmic reticulum chaperone BiP precursor

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	hsp-4	NP_495536.1
Schizosaccharomyces pombe (fission yeast)	bip1	NP_593245.1
Arabidopsis thaliana (thale cress)	BIP1	NP_198206.1
Homo sapiens (human)	HSPA5	NP_005338.1
Rattus norvegicus (Norway rat)	Hspa5	NP_037215.1
Danio rerio (zebrafish)	hspa5	NP_998223.1
Neurospora crassa	NCU03982	XP_956567.1
Xenopus tropicalis (tropical clawed frog)	LOC100492570	XP_002941690.1
	HSPA5	XP_001099110.1
Canis lupus familiaris (dog)	HSPA5	XP_863385.2
Bos taurus (cattle)	HSPA5	NP_001068616.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004192	XP_313085.3
Eremothecium gossypii	AGOS_ACR038W	NP_983441.1
Caenorhabditis elegans (roundworm)	hsp-3	NP_509019.1
Saccharomyces cerevisiae (baker's yeast)	KAR2	NP_012500.3
Kluyveromyces lactis	KLLA0D09559g	XP_453488.1
Arabidopsis thaliana (thale cress)	BIP2	NP_851119.1
Pan troglodytes (chimpanzee)	HSPA5	XP_520257.2
Mus musculus (house mouse)	Hspa5	NP_001156906.1
Gallus gallus (chicken)	HSPA5	NP_990822.1
Drosophila melanogaster (fruit fly)	Hsc70-3	NP_727564.1
Magnaporthe oryzae (rice blast fungus)	MGG_02503	XP_003709308.1
Oryza sativa (rice)	Os02g0115900	NP_001045675.1

Gene Ontology
Bibliography

Related articles in PubMed

Membrane-associated GRP78 helps subgroup J avian leucosis virus enter cells.
Wang L, Mei M, Qin A, Ye J, Qian K, Shao H
Veterinary research47(1)92(2016 09)

GRP78 is required for cell proliferation and protection from apoptosis in chicken embryo fibroblast cells.
Jeon M, Choi H, Lee SI, Kim JS, Park M, Kim K, Lee S, Byun SJ
Poultry science95(5)1129-36(2016 May)

Association analysis between SNPs in the 5'-flanking region of the chicken GRP78 gene, thermotolerance parameters, and tissue mRNA expression.
Kong LN, Zhang DX, Ji CL, Zhang XQ, Luo QB
Genetics and molecular research : GMR14(2)6110-23(2015 Jun)

Avian reovirus S1133-induced apoptosis is associated with Bip/GRP79-mediated Bim translocation to the endoplasmic reticulum.
Lin PY, Liu HJ, Chang CD, Chen YC, Chang CI, Shih WL
Apoptosis : an international journal on programmed cell death20(4)481-90(2015 Apr)

Conserved BK channel-protein interactions reveal signals relevant to cell death and survival.
Sokolowski B, Orchard S, Harvey M, Sridhar S, Sakai Y
PloS one6(12)e28532(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

helps subgroup J avian leucosis virus enter cells
Title: Membrane-associated GRP78 helps subgroup J avian leucosis virus enter cells.

These findings have important implications for the maintenance of chicken fibroblast cells through the inhibition of apoptosis and may lead to the development of new treatments for avian disease.
Title: GRP78 is required for cell proliferation and protection from apoptosis in chicken embryo fibroblast cells.

Data indicate that mRNA expression level of GRP78 gene increased gradually under heat stress then decreased after a certain time suggesting that GRP78 mRNA expression is time- and tissue-dependent.
Title: Association analysis between SNPs in the 5'-flanking region of the chicken GRP78 gene, thermotolerance parameters, and tissue mRNA expression.

BiP/GRP78 played critical roles and works upstream of JNK-Bim in response to the ARV S1133-mediated apoptosis process.
Title: Avian reovirus S1133-induced apoptosis is associated with Bip/GRP79-mediated Bim translocation to the endoplasmic reticulum.

The following HSPA5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSPA5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa28069	NM_205491.1 Latest version!	Gallus gallus heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSPA5 ( NM_205491.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990822.1 Gallus gallus heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5), mRNA.

Price & Availability↑

CloneID	OGa28069
Clone ID Related Accession (Same CDS sequence)	NM_205491.1
Accession Version	NM_205491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Gallus gallus(chicken)
Product	endoplasmic reticulum chaperone BiP precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M27260.1. ##Evidence-Data-START## Transcript exon combination :: M27260.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGGCACC TCCTGTTGGC GCTGCTGCTG CTGGGCGGCG CGCGGGCGGA CGATGAGGAG 
AAAAAGGAGG ACGTGGGCAC GGTGGTGGGC ATCGACCTCG GTACCACCTA TTCTTGTGTG 
GGTGTATTCA AGAACGGCCG CGTGGAAATC ATTGCCAATG ACCAGGGGAA CCGCATCACG 
CCATCCTACG TGGCGTTCAC GCCTGAGGGG GAGCGCCTGA TTGGGGATGC AGCCAAGAAC 
CAGCTGACAT CCAACCCTGA GAACACTGTG TTTGATGCCA AGCGGCTCAT AGGCCGCACC 
TGGAATGACC CCTCTGTACA GCAGGACATC AAGTATCTGC CCTTCAAGGT TGTTGAAAAG 
AAGGCCAAGC CACATATTCA GGTCGATGTT GGTGGTGGAC AAACAAAAAC ATTTGCTCCT 
GAAGAAATTT CTGCCATGGT CCTGACAAAG ATGAAAGAAA CTGCAGAGGC TTATCTGGGC 
AAGAAAGTTA CTCATGCTGT TGTTACTGTG CCAGCCTACT TCAATGATGC TCAGCGTCAG 
GCCACAAAAG ATGCTGGTAC CATTGCTGGG TTGAACGTGA TGCGCATTAT TAATGAGCCA 
ACTGCTGCTG CAATTGCATA CGGATTGGAC AAGAGAGAGG GTGAAAAGAA CATCCTTGTA 
TTTGACCTGG GTGGTGGAAC TTTTGATGTC TCCCTCCTGA CAATTGACAA CGGAGTCTTT 
GAAGTTGTGG CTACAAATGG TGACACACAC CTGGGTGGAG AAGACTTTGA CCAGCGTGTT 
ATGGAGCACT TCATCAAACT CTACAAGAAG AAAACAGGAA AAGATGTCAG GAAGGATAAC 
AGAGCTGTAC AGAAACTAAG ACGGGAAGTA GAGAAAGCGA AGCGGGCCCT GTCATCCCAG 
CACCAAGCTA GAATTGAAAT AGAATCCTTT TTTGAAGGAG AGGATTTCTC TGAGACGCTT 
ACTCGTGCCA AATTTGAAGA ACTGAATATG GATCTGTTCC GTTCTACGAT GAAGCCTGTT 
CAGAAAGTTC TGGAAGACTC TGACCTAAAG AAGTCTGATA TTGATGAGAT TGTTCTTGTT 
GGTGGTTCTA CTCGCATCCC CAAAATACAA CAACTTGTTA AAGAGTTCTT CAATGGGAAA 
GAGCCTTCTC GTGGCATTAA TCCAGATGAA GCTGTAGCCT ATGGTGCAGC TGTTCAGGCT 
GGTGTTCTCT CTGGGGACCA AGACACAGGT GACCTGGTGT TGCTTGATGT GTGTCCTCTG 
ACACTTGGCA TTGAGACAGT TGGAGGTGTA ATGACTAAAC TGATCCCAAG AAATACTGTT 
GTTCCTACCA AGAAGTCTCA GATCTTTTCC ACAGCTTCTG ACAATCAGCC CACTGTGACC 
ATTAAGGTCT ATGAAGGTGA ACGTCCCCTC ACCAAGGACA ATCATCTTCT GGGAACTTTT 
GATCTGACTG GAATCCCTCC TGCTCCTCGT GGTGTCCCAC AGATTGAAGT TACCTTTGAA 
ATAGATGTGA ATGGAATCCT TCGTGTCACA GCTGAAGACA AAGGCACTGG GAACAAAAAC 
AAGATCACAA TTACAAATGA TCAGAATCGG CTAACACCAG AGGAGATTGA GAGGATGGTT 
AATGATGCTG AGAAGTTTGC TGAGGAAGAC AAAAAGCTTA AAGAACGCAT TGATGCCCGG 
AATGAGCTGG AGAGCTATGC GTATTCTTTG AAGAATCAAA TTGGGGACAA AGAGAAGCTG 
GGTGGCAAGC TGTCATCTGA AGACAAAGAA ACAATAGAAA AGGCAGTAGA GGAAAAGATA 
GAGTGGCTTG AAAGCCATCA GGATGCAGAC ATTGAAGACT TCAAATCAAA GAAGAAGGAG 
CTGGAGGAAG TTGTTCAACC AATTGTTAGC AAGCTCTATG GAAGTGCAGG CCCCCCTCCC 
ACTGGTGAAG AAGAAGCAGC AGAGAAGGAT GAGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990822.1
CDS	44..2002
Translation	MRHLLLALLLLGGARADDEEKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKYLPFKVVEKKAKPHIQVDVGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDARNELESYAYSLKNQIGDKEKLGGKLSSEDKETIEKAVEEKIEWLESHQDADIEDFKSKKKELEEVVQPIVSKLYGSAGPPPTGEEEAAEKDEL

Target ORF information:

RefSeq Version	NM_205491.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205491.1

ORF Insert Sequence:

ATGAGGCACC TCCTGTTGGC GCTGCTGCTG CTGGGCGGCG CGCGGGCGGA CGATGAGGAG 
AAAAAGGAGG ACGTGGGCAC GGTGGTGGGC ATCGACCTCG GTACCACCTA TTCTTGTGTG 
GGTGTATTCA AGAACGGCCG CGTGGAAATC ATTGCCAATG ACCAGGGGAA CCGCATCACG 
CCATCCTACG TGGCGTTCAC GCCTGAGGGG GAGCGCCTGA TTGGGGATGC AGCCAAGAAC 
CAGCTGACAT CCAACCCTGA GAACACTGTG TTTGATGCCA AGCGGCTCAT AGGCCGCACC 
TGGAATGACC CCTCTGTACA GCAGGACATC AAGTATCTGC CCTTCAAGGT TGTTGAAAAG 
AAGGCCAAGC CACATATTCA GGTCGATGTT GGTGGTGGAC AAACAAAAAC ATTTGCTCCT 
GAAGAAATTT CTGCCATGGT CCTGACAAAG ATGAAAGAAA CTGCAGAGGC TTATCTGGGC 
AAGAAAGTTA CTCATGCTGT TGTTACTGTG CCAGCCTACT TCAATGATGC TCAGCGTCAG 
GCCACAAAAG ATGCTGGTAC CATTGCTGGG TTGAACGTGA TGCGCATTAT TAATGAGCCA 
ACTGCTGCTG CAATTGCATA CGGATTGGAC AAGAGAGAGG GTGAAAAGAA CATCCTTGTA 
TTTGACCTGG GTGGTGGAAC TTTTGATGTC TCCCTCCTGA CAATTGACAA CGGAGTCTTT 
GAAGTTGTGG CTACAAATGG TGACACACAC CTGGGTGGAG AAGACTTTGA CCAGCGTGTT 
ATGGAGCACT TCATCAAACT CTACAAGAAG AAAACAGGAA AAGATGTCAG GAAGGATAAC 
AGAGCTGTAC AGAAACTAAG ACGGGAAGTA GAGAAAGCGA AGCGGGCCCT GTCATCCCAG 
CACCAAGCTA GAATTGAAAT AGAATCCTTT TTTGAAGGAG AGGATTTCTC TGAGACGCTT 
ACTCGTGCCA AATTTGAAGA ACTGAATATG GATCTGTTCC GTTCTACGAT GAAGCCTGTT 
CAGAAAGTTC TGGAAGACTC TGACCTAAAG AAGTCTGATA TTGATGAGAT TGTTCTTGTT 
GGTGGTTCTA CTCGCATCCC CAAAATACAA CAACTTGTTA AAGAGTTCTT CAATGGGAAA 
GAGCCTTCTC GTGGCATTAA TCCAGATGAA GCTGTAGCCT ATGGTGCAGC TGTTCAGGCT 
GGTGTTCTCT CTGGGGACCA AGACACAGGT GACCTGGTGT TGCTTGATGT GTGTCCTCTG 
ACACTTGGCA TTGAGACAGT TGGAGGTGTA ATGACTAAAC TGATCCCAAG AAATACTGTT 
GTTCCTACCA AGAAGTCTCA GATCTTTTCC ACAGCTTCTG ACAATCAGCC CACTGTGACC 
ATTAAGGTCT ATGAAGGTGA ACGTCCCCTC ACCAAGGACA ATCATCTTCT GGGAACTTTT 
GATCTGACTG GAATCCCTCC TGCTCCTCGT GGTGTCCCAC AGATTGAAGT TACCTTTGAA 
ATAGATGTGA ATGGAATCCT TCGTGTCACA GCTGAAGACA AAGGCACTGG GAACAAAAAC 
AAGATCACAA TTACAAATGA TCAGAATCGG CTAACACCAG AGGAGATTGA GAGGATGGTT 
AATGATGCTG AGAAGTTTGC TGAGGAAGAC AAAAAGCTTA AAGAACGCAT TGATGCCCGG 
AATGAGCTGG AGAGCTATGC GTATTCTTTG AAGAATCAAA TTGGGGACAA AGAGAAGCTG 
GGTGGCAAGC TGTCATCTGA AGACAAAGAA ACAATAGAAA AGGCAGTAGA GGAAAAGATA 
GAGTGGCTTG AAAGCCATCA GGATGCAGAC ATTGAAGACT TCAAATCAAA GAAGAAGGAG 
CTGGAGGAAG TTGTTCAACC AATTGTTAGC AAGCTCTATG GAAGTGCAGG CCCCCCTCCC 
ACTGGTGAAG AAGAAGCAGC AGAGAAGGAT GAGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Membrane-associated GRP78 helps subgroup J avian leucosis virus enter cells.
Veterinary research47(1)92(2016 09)
Wang L,Mei M,Qin A,Ye J,Qian K,Shao H

GRP78 is required for cell proliferation and protection from apoptosis in chicken embryo fibroblast cells.
Poultry science95(5)1129-36(2016 May)
Jeon M,Choi H,Lee SI,Kim JS,Park M,Kim K,Lee S,Byun SJ

Association analysis between SNPs in the 5'-flanking region of the chicken GRP78 gene, thermotolerance parameters, and tissue mRNA expression.
Genetics and molecular research : GMR14(2)6110-23(2015 Jun)
Kong LN,Zhang DX,Ji CL,Zhang XQ,Luo QB

Avian reovirus S1133-induced apoptosis is associated with Bip/GRP79-mediated Bim translocation to the endoplasmic reticulum.
Apoptosis : an international journal on programmed cell death20(4)481-90(2015 Apr)
Lin PY,Liu HJ,Chang CD,Chen YC,Chang CI,Shih WL

Conserved BK channel-protein interactions reveal signals relevant to cell death and survival.
PloS one6(12)e28532(2011)
Sokolowski B,Orchard S,Harvey M,Sridhar S,Sakai Y

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HSPA5 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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