IFIH1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	IFIH1
Entrez Gene ID	102410252
Full Name	interferon induced with helicase C domain 1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006073992.1	XP_006074054.1	interferon-induced helicase C domain-containing protein 1
XM_006073992.2	XP_006074054.1	interferon-induced helicase C domain-containing protein 1

Pathways from BioSystems
Gene Ontology

The following IFIH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IFIH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu27970	XM_006073992.2 Latest version!	Bubalus bubalis interferon induced with helicase C domain 1 (IFIH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OBu27970	XM_006073992.1	Bubalus bubalis interferon induced with helicase C domain 1 (IFIH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IFIH1 ( XM_006073992.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006074054.1 Bubalus bubalis interferon induced with helicase C domain 1 (IFIH1), mRNA.

Price & Availability↑

CloneID	OBu27970
Clone ID Related Accession (Same CDS sequence)	XM_006073992.1 , XM_006073992.2
Accession Version	XM_006073992.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	interferon-induced helicase C domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785567.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCGG ACGGGAGTTT CGCAGACAAG AATTTCTGCT ATCTCATCTC GTGTTTCAGG 
GCCAGAGTGA AAAGGTACAT CCAGGTGGAA CCAGTATTGG ACTACCTGAC TTTTCTATCT 
CCAGAAGTGA AAGAGCATAT TCAGAGGACG GCCGCCACCA CTGGGGACAT TCAGGCAGCT 
GATCTGCTTC TGGACACCTT GGAGAGGGGG AACTGGCCGC TCGGCTGGGC TAGGATGTTC 
GTAGAGGCCC TCCGGCAAGC AGGCAACCCC TTAGCCGCCC GCTACGTGAA CCCAGAGCTC 
ACGGACTTGC CTTCTCCATC GTTTGAGAAC GCTCACGATG AATGTCTCCA ACTGCTGAAC 
CTTCTTCAGC CCACACTGGT GGACAAGCTT CTGGTTGCAG ATGTCTTGGA TAAATGTGTG 
GAGGAAAAAC TGTTGACAAT TGAAGACAGA AATCGGGTTT CTGCTGCAGA AAATAATGGA 
AATGAAGCAG GGGTAAGAGA GCTACTGAAA AGGATTGTGC AGAAAGAAAA CTGGTTTTCT 
ACATTTCTGA CTATTCTGAG GCAGACGGGA AATGATGAGC TTGCCCGGGA GTTAACAGGC 
ACTGATTGCT GTGAAGGCAA TACAGAGTCT GAGAATTTAT CACAAGAAGA TGGTCTCGAA 
GTCAAAGAGC CACTCCTTTT AGCCACAGAT CAACCAAATC TGGAGGTCTT GGACATAGAG 
AATAGTTCAC TGGAATCGTC TTTTGCAGAT TCTTCTATAG TTTCAGAATC AGACACAAGT 
TTGGCAGAAG GAAGTGTCAG CTGCTTAGAT GAAAGTCTTG GACATAACAG CAACATGGGC 
AGTGATTCAG GCACCATGGG AAGTGATTCA GATGATGAGA ATGTGGCTCA AAGAGCATCC 
CCCGAGCCAG AGCTGAATCT CAGGCCTTAC CAGCTGGAAG TGGCCCAGCC TGCCCTGGAG 
GGGAAGAACA TCATTATATG CCTCCCTACA GGGAGTGGGA AAACCAGAGT GGCCGTTTAC 
ATTGCCAAGG ATCACTTGGA CAAGAAAAAA GCATCTGAAC CTGGAAAAGT TATGGTTCTC 
GTCAATAAGG TACCGTTAGT TGAACAGCTC TTCCGCAAAG AGTTCAAACC ATTTTTGAAG 
AAATGGTATC ATGTTACTAG ATTAAGTGGT GATACCCAAT TGAAAATAAC ATTTCCAGAA 
GTTGTCAAGT CTCATGATGT TATTATCAGT ACAGCTCAAA TCCTTGAAAA CTCCCTTTTA 
AACTCAGAAG AAGGTGAAGA TGATGGTATA GAGTTGTCAG ACTTTTCTCT CATCATTATT 
GATGAATGCC ATCACACCAA CAAAGAAGCG GTCTATAACA ACATCATGAG GCGTTATTTG 
AAACAGAAGT TGAAAAACAA TAAACTTAAG AAAGAAAACA AACCAGTGAT TCCCTTACCT 
CAGATAGTGG GACTAACAGC CTCACCAGGT GTTGGAGGAG CCAAAAAGCA AGCCAAAGCA 
GAAGAACACA TTTTAAAAAT ATGTGCCAAT CTTGATGCAG TTACCATTAA AACCGTGAAA 
CAAAATATTA ATCTACTTAA AGAGCAAATA AAGGAGCCAT GCAAAAAGTT TGTAATTGCG 
GATGATACCA AAAAAGATCC ATTTAAAGAT AAGCTGCTAG AAATAATGAC AAAGATTCAA 
ACATTTTGCC AAATTAATCC AATGTCAGAT TTTGGAACTC AACCCTATGA ACAATGGCTC 
ATTCAAATGG AAAAAAAAGC TGCAAAAGAA GGAAATCGCA AAGATCGTGT TTGTGCAGAA 
CATCTGAGGA AGTACAATGA GGCCCTACAA ATTAATGATA CAATCCGTAT GATTGATGCA 
TATAATCACC TTGAAACTTT CTACAATGAC GAGAAAGAGA AGAAGTTTGC GGTCCTCCTA 
GGAGATGACA GTGATGAGAG CGGGGACAAT GGTGATGATG ATGCGGGTGA TGGAAAAGCA 
CCTTTGAAGC TGCACGAGAC AGATGACTTT CTCATATCTC TATTTTTGGG AAATAAGAAA 
AAGTTGAAAA AACTAGCCCA AAACCCAGAA CATGAAAATG AAAAGCTCAT CAAATTGAGA 
AACACCATAA TGGAACAATA TTCAAGGACT GAAGGATCAG CACGAGGAAT AATTTTCACA 
AAAACACGAC AGAGTGCATA TGCCCTTTCC CAGTGGATTA TTGAAAACGA AAAATTTTCA 
GAAGTAGGAG TCAAAGCCCA CCATCTTATT GGCGCTGGAC ACAGCAGCGA GTTCAAGCCC 
ATGACACAGA ATGAACAAAA AGAAGTCATT AGTAAATTTC GCACGGGAAA AATAAATCTG 
CTTATCGCTA CCACAGTGGC AGAAGAAGGT CTGGATATTA AAGAATGTAA TATCGTTATC 
CGTTATGGTC TCGTCACAAA TGAAATAGCC ATGGTCCAGG CCCGTGGTCG AGCCAGAGCT 
GATGAGAGCA CCTATGTCCT CGTTGCCCAA AGCGGCTCAG GGGTTGTTGA ACGTGAGACA 
GTTAATGATT TCCGAGAGAA AATGATGTAT AAAGCTATAG ACCGTGTTCA AAATATGAAA 
CCAGAGGAGT ATGCTCATAA GATTTTGGAA TTACAGATGC AAAGTATAAT GGAAAAGAAA 
ATGAAAACCA AGAGAAGTAT TGCAAAGCAG TTCAAGGACA AGCCATCATT AATAAATTTT 
CTGTGTAAAA ACTGTGGTGT GCCAGCCTGC TCTGGAGAAG ACATCCATGT AATTGAGAAG 
ATGCACCACG TCAATATGAC ACCAGAGTTC AAGAAACTGT ACTTGGTGAG AGGAAACAAA 
GCACTGCAAA CAATGTGTGT TGATTATCAA ACAAATGGCG AGATAATCTG CAATAAATGT 
GGCCAAGCTT GGGGAACGAT GATGGTGCAC AAAGGCTTAG ATTTGCCTTG TCTCAAAATA 
AAGAATTTTG TAGTGGTTTT CCAAAATAAT TTACCAAAGA AACAATACAA AAAGTGGGTA 
GAATTACCAA TCACATTTCC TGATCTTAAC TATTCGGAAT ATTGTTTGTT TAGTGATGAG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006074054.1
CDS	134..3199
Translation	MSSDGSFADKNFCYLISCFRARVKRYIQVEPVLDYLTFLSPEVKEHIQRTAATTGDIQAADLLLDTLERGNWPLGWARMFVEALRQAGNPLAARYVNPELTDLPSPSFENAHDECLQLLNLLQPTLVDKLLVADVLDKCVEEKLLTIEDRNRVSAAENNGNEAGVRELLKRIVQKENWFSTFLTILRQTGNDELARELTGTDCCEGNTESENLSQEDGLEVKEPLLLATDQPNLEVLDIENSSLESSFADSSIVSESDTSLAEGSVSCLDESLGHNSNMGSDSGTMGSDSDDENVAQRASPEPELNLRPYQLEVAQPALEGKNIIICLPTGSGKTRVAVYIAKDHLDKKKASEPGKVMVLVNKVPLVEQLFRKEFKPFLKKWYHVTRLSGDTQLKITFPEVVKSHDVIISTAQILENSLLNSEEGEDDGIELSDFSLIIIDECHHTNKEAVYNNIMRRYLKQKLKNNKLKKENKPVIPLPQIVGLTASPGVGGAKKQAKAEEHILKICANLDAVTIKTVKQNINLLKEQIKEPCKKFVIADDTKKDPFKDKLLEIMTKIQTFCQINPMSDFGTQPYEQWLIQMEKKAAKEGNRKDRVCAEHLRKYNEALQINDTIRMIDAYNHLETFYNDEKEKKFAVLLGDDSDESGDNGDDDAGDGKAPLKLHETDDFLISLFLGNKKKLKKLAQNPEHENEKLIKLRNTIMEQYSRTEGSARGIIFTKTRQSAYALSQWIIENEKFSEVGVKAHHLIGAGHSSEFKPMTQNEQKEVISKFRTGKINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVAQSGSGVVERETVNDFREKMMYKAIDRVQNMKPEEYAHKILELQMQSIMEKKMKTKRSIAKQFKDKPSLINFLCKNCGVPACSGEDIHVIEKMHHVNMTPEFKKLYLVRGNKALQTMCVDYQTNGEIICNKCGQAWGTMMVHKGLDLPCLKIKNFVVVFQNNLPKKQYKKWVELPITFPDLNYSEYCLFSDED

Target ORF information:

RefSeq Version	XM_006073992.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis interferon induced with helicase C domain 1 (IFIH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073992.1

ORF Insert Sequence:

ATGTCGTCGG ACGGGAGTTT CGCAGACAAG AATTTCTGCT ATCTCATCTC GTGTTTCAGG 
GCCAGAGTGA AAAGGTACAT CCAGGTGGAA CCAGTATTGG ACTACCTGAC TTTTCTATCT 
CCAGAAGTGA AAGAGCATAT TCAGAGGACG GCCGCCACCA CTGGGGACAT TCAGGCAGCT 
GATCTGCTTC TGGACACCTT GGAGAGGGGG AACTGGCCGC TCGGCTGGGC TAGGATGTTC 
GTAGAGGCCC TCCGGCAAGC AGGCAACCCC TTAGCCGCCC GCTACGTGAA CCCAGAGCTC 
ACGGACTTGC CTTCTCCATC GTTTGAGAAC GCTCACGATG AATGTCTCCA ACTGCTGAAC 
CTTCTTCAGC CCACACTGGT GGACAAGCTT CTGGTTGCAG ATGTCTTGGA TAAATGTGTG 
GAGGAAAAAC TGTTGACAAT TGAAGACAGA AATCGGGTTT CTGCTGCAGA AAATAATGGA 
AATGAAGCAG GGGTAAGAGA GCTACTGAAA AGGATTGTGC AGAAAGAAAA CTGGTTTTCT 
ACATTTCTGA CTATTCTGAG GCAGACGGGA AATGATGAGC TTGCCCGGGA GTTAACAGGC 
ACTGATTGCT GTGAAGGCAA TACAGAGTCT GAGAATTTAT CACAAGAAGA TGGTCTCGAA 
GTCAAAGAGC CACTCCTTTT AGCCACAGAT CAACCAAATC TGGAGGTCTT GGACATAGAG 
AATAGTTCAC TGGAATCGTC TTTTGCAGAT TCTTCTATAG TTTCAGAATC AGACACAAGT 
TTGGCAGAAG GAAGTGTCAG CTGCTTAGAT GAAAGTCTTG GACATAACAG CAACATGGGC 
AGTGATTCAG GCACCATGGG AAGTGATTCA GATGATGAGA ATGTGGCTCA AAGAGCATCC 
CCCGAGCCAG AGCTGAATCT CAGGCCTTAC CAGCTGGAAG TGGCCCAGCC TGCCCTGGAG 
GGGAAGAACA TCATTATATG CCTCCCTACA GGGAGTGGGA AAACCAGAGT GGCCGTTTAC 
ATTGCCAAGG ATCACTTGGA CAAGAAAAAA GCATCTGAAC CTGGAAAAGT TATGGTTCTC 
GTCAATAAGG TACCGTTAGT TGAACAGCTC TTCCGCAAAG AGTTCAAACC ATTTTTGAAG 
AAATGGTATC ATGTTACTAG ATTAAGTGGT GATACCCAAT TGAAAATAAC ATTTCCAGAA 
GTTGTCAAGT CTCATGATGT TATTATCAGT ACAGCTCAAA TCCTTGAAAA CTCCCTTTTA 
AACTCAGAAG AAGGTGAAGA TGATGGTATA GAGTTGTCAG ACTTTTCTCT CATCATTATT 
GATGAATGCC ATCACACCAA CAAAGAAGCG GTCTATAACA ACATCATGAG GCGTTATTTG 
AAACAGAAGT TGAAAAACAA TAAACTTAAG AAAGAAAACA AACCAGTGAT TCCCTTACCT 
CAGATAGTGG GACTAACAGC CTCACCAGGT GTTGGAGGAG CCAAAAAGCA AGCCAAAGCA 
GAAGAACACA TTTTAAAAAT ATGTGCCAAT CTTGATGCAG TTACCATTAA AACCGTGAAA 
CAAAATATTA ATCTACTTAA AGAGCAAATA AAGGAGCCAT GCAAAAAGTT TGTAATTGCG 
GATGATACCA AAAAAGATCC ATTTAAAGAT AAGCTGCTAG AAATAATGAC AAAGATTCAA 
ACATTTTGCC AAATTAATCC AATGTCAGAT TTTGGAACTC AACCCTATGA ACAATGGCTC 
ATTCAAATGG AAAAAAAAGC TGCAAAAGAA GGAAATCGCA AAGATCGTGT TTGTGCAGAA 
CATCTGAGGA AGTACAATGA GGCCCTACAA ATTAATGATA CAATCCGTAT GATTGATGCA 
TATAATCACC TTGAAACTTT CTACAATGAC GAGAAAGAGA AGAAGTTTGC GGTCCTCCTA 
GGAGATGACA GTGATGAGAG CGGGGACAAT GGTGATGATG ATGCGGGTGA TGGAAAAGCA 
CCTTTGAAGC TGCACGAGAC AGATGACTTT CTCATATCTC TATTTTTGGG AAATAAGAAA 
AAGTTGAAAA AACTAGCCCA AAACCCAGAA CATGAAAATG AAAAGCTCAT CAAATTGAGA 
AACACCATAA TGGAACAATA TTCAAGGACT GAAGGATCAG CACGAGGAAT AATTTTCACA 
AAAACACGAC AGAGTGCATA TGCCCTTTCC CAGTGGATTA TTGAAAACGA AAAATTTTCA 
GAAGTAGGAG TCAAAGCCCA CCATCTTATT GGCGCTGGAC ACAGCAGCGA GTTCAAGCCC 
ATGACACAGA ATGAACAAAA AGAAGTCATT AGTAAATTTC GCACGGGAAA AATAAATCTG 
CTTATCGCTA CCACAGTGGC AGAAGAAGGT CTGGATATTA AAGAATGTAA TATCGTTATC 
CGTTATGGTC TCGTCACAAA TGAAATAGCC ATGGTCCAGG CCCGTGGTCG AGCCAGAGCT 
GATGAGAGCA CCTATGTCCT CGTTGCCCAA AGCGGCTCAG GGGTTGTTGA ACGTGAGACA 
GTTAATGATT TCCGAGAGAA AATGATGTAT AAAGCTATAG ACCGTGTTCA AAATATGAAA 
CCAGAGGAGT ATGCTCATAA GATTTTGGAA TTACAGATGC AAAGTATAAT GGAAAAGAAA 
ATGAAAACCA AGAGAAGTAT TGCAAAGCAG TTCAAGGACA AGCCATCATT AATAAATTTT 
CTGTGTAAAA ACTGTGGTGT GCCAGCCTGC TCTGGAGAAG ACATCCATGT AATTGAGAAG 
ATGCACCACG TCAATATGAC ACCAGAGTTC AAGAAACTGT ACTTGGTGAG AGGAAACAAA 
GCACTGCAAA CAATGTGTGT TGATTATCAA ACAAATGGCG AGATAATCTG CAATAAATGT 
GGCCAAGCTT GGGGAACGAT GATGGTGCAC AAAGGCTTAG ATTTGCCTTG TCTCAAAATA 
AAGAATTTTG TAGTGGTTTT CCAAAATAAT TTACCAAAGA AACAATACAA AAAGTGGGTA 
GAATTACCAA TCACATTTCC TGATCTTAAC TATTCGGAAT ATTGTTTGTT TAGTGATGAG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFIH1 ( XM_006073992.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006074054.1 Bubalus bubalis interferon induced with helicase C domain 1 (IFIH1), mRNA.

Price & Availability↑

CloneID	OBu27970
Clone ID Related Accession (Same CDS sequence)	XM_006073992.1 , XM_006073992.2
Accession Version	XM_006073992.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	interferon-induced helicase C domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006073992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCGG ACGGGAGTTT CGCAGACAAG AATTTCTGCT ATCTCATCTC GTGTTTCAGG 
GCCAGAGTGA AAAGGTACAT CCAGGTGGAA CCAGTATTGG ACTACCTGAC TTTTCTATCT 
CCAGAAGTGA AAGAGCATAT TCAGAGGACG GCCGCCACCA CTGGGGACAT TCAGGCAGCT 
GATCTGCTTC TGGACACCTT GGAGAGGGGG AACTGGCCGC TCGGCTGGGC TAGGATGTTC 
GTAGAGGCCC TCCGGCAAGC AGGCAACCCC TTAGCCGCCC GCTACGTGAA CCCAGAGCTC 
ACGGACTTGC CTTCTCCATC GTTTGAGAAC GCTCACGATG AATGTCTCCA ACTGCTGAAC 
CTTCTTCAGC CCACACTGGT GGACAAGCTT CTGGTTGCAG ATGTCTTGGA TAAATGTGTG 
GAGGAAAAAC TGTTGACAAT TGAAGACAGA AATCGGGTTT CTGCTGCAGA AAATAATGGA 
AATGAAGCAG GGGTAAGAGA GCTACTGAAA AGGATTGTGC AGAAAGAAAA CTGGTTTTCT 
ACATTTCTGA CTATTCTGAG GCAGACGGGA AATGATGAGC TTGCCCGGGA GTTAACAGGC 
ACTGATTGCT GTGAAGGCAA TACAGAGTCT GAGAATTTAT CACAAGAAGA TGGTCTCGAA 
GTCAAAGAGC CACTCCTTTT AGCCACAGAT CAACCAAATC TGGAGGTCTT GGACATAGAG 
AATAGTTCAC TGGAATCGTC TTTTGCAGAT TCTTCTATAG TTTCAGAATC AGACACAAGT 
TTGGCAGAAG GAAGTGTCAG CTGCTTAGAT GAAAGTCTTG GACATAACAG CAACATGGGC 
AGTGATTCAG GCACCATGGG AAGTGATTCA GATGATGAGA ATGTGGCTCA AAGAGCATCC 
CCCGAGCCAG AGCTGAATCT CAGGCCTTAC CAGCTGGAAG TGGCCCAGCC TGCCCTGGAG 
GGGAAGAACA TCATTATATG CCTCCCTACA GGGAGTGGGA AAACCAGAGT GGCCGTTTAC 
ATTGCCAAGG ATCACTTGGA CAAGAAAAAA GCATCTGAAC CTGGAAAAGT TATGGTTCTC 
GTCAATAAGG TACCGTTAGT TGAACAGCTC TTCCGCAAAG AGTTCAAACC ATTTTTGAAG 
AAATGGTATC ATGTTACTAG ATTAAGTGGT GATACCCAAT TGAAAATAAC ATTTCCAGAA 
GTTGTCAAGT CTCATGATGT TATTATCAGT ACAGCTCAAA TCCTTGAAAA CTCCCTTTTA 
AACTCAGAAG AAGGTGAAGA TGATGGTATA GAGTTGTCAG ACTTTTCTCT CATCATTATT 
GATGAATGCC ATCACACCAA CAAAGAAGCG GTCTATAACA ACATCATGAG GCGTTATTTG 
AAACAGAAGT TGAAAAACAA TAAACTTAAG AAAGAAAACA AACCAGTGAT TCCCTTACCT 
CAGATAGTGG GACTAACAGC CTCACCAGGT GTTGGAGGAG CCAAAAAGCA AGCCAAAGCA 
GAAGAACACA TTTTAAAAAT ATGTGCCAAT CTTGATGCAG TTACCATTAA AACCGTGAAA 
CAAAATATTA ATCTACTTAA AGAGCAAATA AAGGAGCCAT GCAAAAAGTT TGTAATTGCG 
GATGATACCA AAAAAGATCC ATTTAAAGAT AAGCTGCTAG AAATAATGAC AAAGATTCAA 
ACATTTTGCC AAATTAATCC AATGTCAGAT TTTGGAACTC AACCCTATGA ACAATGGCTC 
ATTCAAATGG AAAAAAAAGC TGCAAAAGAA GGAAATCGCA AAGATCGTGT TTGTGCAGAA 
CATCTGAGGA AGTACAATGA GGCCCTACAA ATTAATGATA CAATCCGTAT GATTGATGCA 
TATAATCACC TTGAAACTTT CTACAATGAC GAGAAAGAGA AGAAGTTTGC GGTCCTCCTA 
GGAGATGACA GTGATGAGAG CGGGGACAAT GGTGATGATG ATGCGGGTGA TGGAAAAGCA 
CCTTTGAAGC TGCACGAGAC AGATGACTTT CTCATATCTC TATTTTTGGG AAATAAGAAA 
AAGTTGAAAA AACTAGCCCA AAACCCAGAA CATGAAAATG AAAAGCTCAT CAAATTGAGA 
AACACCATAA TGGAACAATA TTCAAGGACT GAAGGATCAG CACGAGGAAT AATTTTCACA 
AAAACACGAC AGAGTGCATA TGCCCTTTCC CAGTGGATTA TTGAAAACGA AAAATTTTCA 
GAAGTAGGAG TCAAAGCCCA CCATCTTATT GGCGCTGGAC ACAGCAGCGA GTTCAAGCCC 
ATGACACAGA ATGAACAAAA AGAAGTCATT AGTAAATTTC GCACGGGAAA AATAAATCTG 
CTTATCGCTA CCACAGTGGC AGAAGAAGGT CTGGATATTA AAGAATGTAA TATCGTTATC 
CGTTATGGTC TCGTCACAAA TGAAATAGCC ATGGTCCAGG CCCGTGGTCG AGCCAGAGCT 
GATGAGAGCA CCTATGTCCT CGTTGCCCAA AGCGGCTCAG GGGTTGTTGA ACGTGAGACA 
GTTAATGATT TCCGAGAGAA AATGATGTAT AAAGCTATAG ACCGTGTTCA AAATATGAAA 
CCAGAGGAGT ATGCTCATAA GATTTTGGAA TTACAGATGC AAAGTATAAT GGAAAAGAAA 
ATGAAAACCA AGAGAAGTAT TGCAAAGCAG TTCAAGGACA AGCCATCATT AATAAATTTT 
CTGTGTAAAA ACTGTGGTGT GCCAGCCTGC TCTGGAGAAG ACATCCATGT AATTGAGAAG 
ATGCACCACG TCAATATGAC ACCAGAGTTC AAGAAACTGT ACTTGGTGAG AGGAAACAAA 
GCACTGCAAA CAATGTGTGT TGATTATCAA ACAAATGGCG AGATAATCTG CAATAAATGT 
GGCCAAGCTT GGGGAACGAT GATGGTGCAC AAAGGCTTAG ATTTGCCTTG TCTCAAAATA 
AAGAATTTTG TAGTGGTTTT CCAAAATAAT TTACCAAAGA AACAATACAA AAAGTGGGTA 
GAATTACCAA TCACATTTCC TGATCTTAAC TATTCGGAAT ATTGTTTGTT TAGTGATGAG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006074054.1
CDS	182..3247
Translation	MSSDGSFADKNFCYLISCFRARVKRYIQVEPVLDYLTFLSPEVKEHIQRTAATTGDIQAADLLLDTLERGNWPLGWARMFVEALRQAGNPLAARYVNPELTDLPSPSFENAHDECLQLLNLLQPTLVDKLLVADVLDKCVEEKLLTIEDRNRVSAAENNGNEAGVRELLKRIVQKENWFSTFLTILRQTGNDELARELTGTDCCEGNTESENLSQEDGLEVKEPLLLATDQPNLEVLDIENSSLESSFADSSIVSESDTSLAEGSVSCLDESLGHNSNMGSDSGTMGSDSDDENVAQRASPEPELNLRPYQLEVAQPALEGKNIIICLPTGSGKTRVAVYIAKDHLDKKKASEPGKVMVLVNKVPLVEQLFRKEFKPFLKKWYHVTRLSGDTQLKITFPEVVKSHDVIISTAQILENSLLNSEEGEDDGIELSDFSLIIIDECHHTNKEAVYNNIMRRYLKQKLKNNKLKKENKPVIPLPQIVGLTASPGVGGAKKQAKAEEHILKICANLDAVTIKTVKQNINLLKEQIKEPCKKFVIADDTKKDPFKDKLLEIMTKIQTFCQINPMSDFGTQPYEQWLIQMEKKAAKEGNRKDRVCAEHLRKYNEALQINDTIRMIDAYNHLETFYNDEKEKKFAVLLGDDSDESGDNGDDDAGDGKAPLKLHETDDFLISLFLGNKKKLKKLAQNPEHENEKLIKLRNTIMEQYSRTEGSARGIIFTKTRQSAYALSQWIIENEKFSEVGVKAHHLIGAGHSSEFKPMTQNEQKEVISKFRTGKINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVAQSGSGVVERETVNDFREKMMYKAIDRVQNMKPEEYAHKILELQMQSIMEKKMKTKRSIAKQFKDKPSLINFLCKNCGVPACSGEDIHVIEKMHHVNMTPEFKKLYLVRGNKALQTMCVDYQTNGEIICNKCGQAWGTMMVHKGLDLPCLKIKNFVVVFQNNLPKKQYKKWVELPITFPDLNYSEYCLFSDED

Target ORF information:

RefSeq Version	XM_006073992.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis interferon induced with helicase C domain 1 (IFIH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073992.2

ORF Insert Sequence:

ATGTCGTCGG ACGGGAGTTT CGCAGACAAG AATTTCTGCT ATCTCATCTC GTGTTTCAGG 
GCCAGAGTGA AAAGGTACAT CCAGGTGGAA CCAGTATTGG ACTACCTGAC TTTTCTATCT 
CCAGAAGTGA AAGAGCATAT TCAGAGGACG GCCGCCACCA CTGGGGACAT TCAGGCAGCT 
GATCTGCTTC TGGACACCTT GGAGAGGGGG AACTGGCCGC TCGGCTGGGC TAGGATGTTC 
GTAGAGGCCC TCCGGCAAGC AGGCAACCCC TTAGCCGCCC GCTACGTGAA CCCAGAGCTC 
ACGGACTTGC CTTCTCCATC GTTTGAGAAC GCTCACGATG AATGTCTCCA ACTGCTGAAC 
CTTCTTCAGC CCACACTGGT GGACAAGCTT CTGGTTGCAG ATGTCTTGGA TAAATGTGTG 
GAGGAAAAAC TGTTGACAAT TGAAGACAGA AATCGGGTTT CTGCTGCAGA AAATAATGGA 
AATGAAGCAG GGGTAAGAGA GCTACTGAAA AGGATTGTGC AGAAAGAAAA CTGGTTTTCT 
ACATTTCTGA CTATTCTGAG GCAGACGGGA AATGATGAGC TTGCCCGGGA GTTAACAGGC 
ACTGATTGCT GTGAAGGCAA TACAGAGTCT GAGAATTTAT CACAAGAAGA TGGTCTCGAA 
GTCAAAGAGC CACTCCTTTT AGCCACAGAT CAACCAAATC TGGAGGTCTT GGACATAGAG 
AATAGTTCAC TGGAATCGTC TTTTGCAGAT TCTTCTATAG TTTCAGAATC AGACACAAGT 
TTGGCAGAAG GAAGTGTCAG CTGCTTAGAT GAAAGTCTTG GACATAACAG CAACATGGGC 
AGTGATTCAG GCACCATGGG AAGTGATTCA GATGATGAGA ATGTGGCTCA AAGAGCATCC 
CCCGAGCCAG AGCTGAATCT CAGGCCTTAC CAGCTGGAAG TGGCCCAGCC TGCCCTGGAG 
GGGAAGAACA TCATTATATG CCTCCCTACA GGGAGTGGGA AAACCAGAGT GGCCGTTTAC 
ATTGCCAAGG ATCACTTGGA CAAGAAAAAA GCATCTGAAC CTGGAAAAGT TATGGTTCTC 
GTCAATAAGG TACCGTTAGT TGAACAGCTC TTCCGCAAAG AGTTCAAACC ATTTTTGAAG 
AAATGGTATC ATGTTACTAG ATTAAGTGGT GATACCCAAT TGAAAATAAC ATTTCCAGAA 
GTTGTCAAGT CTCATGATGT TATTATCAGT ACAGCTCAAA TCCTTGAAAA CTCCCTTTTA 
AACTCAGAAG AAGGTGAAGA TGATGGTATA GAGTTGTCAG ACTTTTCTCT CATCATTATT 
GATGAATGCC ATCACACCAA CAAAGAAGCG GTCTATAACA ACATCATGAG GCGTTATTTG 
AAACAGAAGT TGAAAAACAA TAAACTTAAG AAAGAAAACA AACCAGTGAT TCCCTTACCT 
CAGATAGTGG GACTAACAGC CTCACCAGGT GTTGGAGGAG CCAAAAAGCA AGCCAAAGCA 
GAAGAACACA TTTTAAAAAT ATGTGCCAAT CTTGATGCAG TTACCATTAA AACCGTGAAA 
CAAAATATTA ATCTACTTAA AGAGCAAATA AAGGAGCCAT GCAAAAAGTT TGTAATTGCG 
GATGATACCA AAAAAGATCC ATTTAAAGAT AAGCTGCTAG AAATAATGAC AAAGATTCAA 
ACATTTTGCC AAATTAATCC AATGTCAGAT TTTGGAACTC AACCCTATGA ACAATGGCTC 
ATTCAAATGG AAAAAAAAGC TGCAAAAGAA GGAAATCGCA AAGATCGTGT TTGTGCAGAA 
CATCTGAGGA AGTACAATGA GGCCCTACAA ATTAATGATA CAATCCGTAT GATTGATGCA 
TATAATCACC TTGAAACTTT CTACAATGAC GAGAAAGAGA AGAAGTTTGC GGTCCTCCTA 
GGAGATGACA GTGATGAGAG CGGGGACAAT GGTGATGATG ATGCGGGTGA TGGAAAAGCA 
CCTTTGAAGC TGCACGAGAC AGATGACTTT CTCATATCTC TATTTTTGGG AAATAAGAAA 
AAGTTGAAAA AACTAGCCCA AAACCCAGAA CATGAAAATG AAAAGCTCAT CAAATTGAGA 
AACACCATAA TGGAACAATA TTCAAGGACT GAAGGATCAG CACGAGGAAT AATTTTCACA 
AAAACACGAC AGAGTGCATA TGCCCTTTCC CAGTGGATTA TTGAAAACGA AAAATTTTCA 
GAAGTAGGAG TCAAAGCCCA CCATCTTATT GGCGCTGGAC ACAGCAGCGA GTTCAAGCCC 
ATGACACAGA ATGAACAAAA AGAAGTCATT AGTAAATTTC GCACGGGAAA AATAAATCTG 
CTTATCGCTA CCACAGTGGC AGAAGAAGGT CTGGATATTA AAGAATGTAA TATCGTTATC 
CGTTATGGTC TCGTCACAAA TGAAATAGCC ATGGTCCAGG CCCGTGGTCG AGCCAGAGCT 
GATGAGAGCA CCTATGTCCT CGTTGCCCAA AGCGGCTCAG GGGTTGTTGA ACGTGAGACA 
GTTAATGATT TCCGAGAGAA AATGATGTAT AAAGCTATAG ACCGTGTTCA AAATATGAAA 
CCAGAGGAGT ATGCTCATAA GATTTTGGAA TTACAGATGC AAAGTATAAT GGAAAAGAAA 
ATGAAAACCA AGAGAAGTAT TGCAAAGCAG TTCAAGGACA AGCCATCATT AATAAATTTT 
CTGTGTAAAA ACTGTGGTGT GCCAGCCTGC TCTGGAGAAG ACATCCATGT AATTGAGAAG 
ATGCACCACG TCAATATGAC ACCAGAGTTC AAGAAACTGT ACTTGGTGAG AGGAAACAAA 
GCACTGCAAA CAATGTGTGT TGATTATCAA ACAAATGGCG AGATAATCTG CAATAAATGT 
GGCCAAGCTT GGGGAACGAT GATGGTGCAC AAAGGCTTAG ATTTGCCTTG TCTCAAAATA 
AAGAATTTTG TAGTGGTTTT CCAAAATAAT TTACCAAAGA AACAATACAA AAAGTGGGTA 
GAATTACCAA TCACATTTCC TGATCTTAAC TATTCGGAAT ATTGTTTGTT TAGTGATGAG 
GACTGA