NLRP8 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	NLRP8
Entrez Gene ID	102404457
Full Name	NLR family pyrin domain containing 8
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025268318.1	XP_025124103.1	NACHT, LRR and PYD domains-containing protein 8
XM_006041796.1	XP_006041858.1	LOW QUALITY PROTEIN: NACHT, LRR and PYD domains-containing protein 8

Pathways from BioSystems
Gene Ontology

The following NLRP8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRP8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu01452	XM_006041796.1 Latest version!	Bubalus bubalis NLR family, pyrin domain containing 8 (NLRP8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu52062	XM_025268318.1 Latest version!	Bubalus bubalis NLR family pyrin domain containing 8 (NLRP8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NLRP8 ( XM_006041796.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006041858.1 Bubalus bubalis NLR family, pyrin domain containing 8 (NLRP8), mRNA.

Price & Availability↑

CloneID	OBu01452
Clone ID Related Accession (Same CDS sequence)	XM_006041796.1
Accession Version	XM_006041796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	LOW QUALITY PROTEIN: NACHT, LRR and PYD domains-containing protein 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005782096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGACA TGAACTTGAA CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
TTTGGTGTCT CCCCTCCCTT GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC 
GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAGAGGTT CAAGCAGCTT 
TTGGTGGAGG AGAACCCCAG ACCCGGCTGC ACCCCGCTCA CCTGGGACCA GCTGAAGTCA 
GCCCGCTGCG GGGAGCTGGT TCATCTGCTG ACCGAGTACT TCCCGGGACA ACGTGCCTGG 
GAGGTGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACACAG 
AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG 
GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG 
AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACAT GGACTTCTTT 
TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC 
CAAGGAAGAC AGCCTAAGAC CGTGGTCCTT CAGGGAGACG CCGGCGTCGG GAAAACCACC 
ATAGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG 
CTCGCTTTCT ACCTCCACTG CCAGGATATG GACCGGCGGG AGGAGCAGAG CTTCTCCGAG 
CTGATCGCCT GCAAGTTGTC CGGGTCTCTG GCTCTCGCGT CTAGGATCCT TTCCCACCCA 
GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA 
CAGGAGGACC TGAGAGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT 
CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG 
TGGAAGACCG TGAGCCCCTT TCTGAACAGT CCCTCTCTGA TCACCCTGAT GGGCTTCAGT 
GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA 
GCCTTGCGTT TCGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC 
TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG 
GCATTCCCCA ACGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACCAGG 
GTGAGGCATG TCATCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT 
GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA CGGACCTCAA CCTGGCCAGA 
CTGGACGAAA GGGACATCGA GGTGTTTCTT GGGGTGAGGG TCCTCCGGAG GGCGGTGGCC 
GGTGGGGACC TCGTCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC TGCCCTGCTA 
TTCGTCCTCT GCTTCCCACA GAGGCTCAGG AATTTCCAAG TGTTGGACCA CTTCCGCATC 
TTACAGCTGC TTGCTCATCC TGGGAGAAGG AAAAACCACC TGGCCGGGAT GGCGCTGTTC 
CTGTTTGGCC TGTTGAATGA CACGTGCGCC CTTGCCGTGG AGCAGCTGTT TGGGTGCAAG 
GTGTCCCTGG GGAACAAGAG GAAACTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC 
CCCCCAACCC CACACCACGG GCTCCCCCAG CTGTTCTACT GTCTGCACGA GATCCGGGAG 
GAGGTCTTCG TGGGTCAGAT CCTCCACGAC CACCGTAAGT CTCTGCTGGT CATCAGCAAG 
ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA 
GAACTGACCA TCGCCTGGGC CGTCGCCAAA GCCACCAGCC TCTTGCCAGG CCCTCTTCCT 
TCCCCCCAAC CAGAAGGCAG TGAGAGGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG 
TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACGGAG 
GCCGTGGAGA CTCTTGCCGC AGCACTGAGG CATCCCCACT GTAACCTTCG GAAACTGATC 
TTTAAGCGTG TGGGCTCTCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC 
CAGTACCTGA GACACTTGGA AATACAAGAC ACGGAAGTGG GATGCCAAGT GATAGACGCT 
CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC 
CCATTCGACC CCACCAACGG AGCTGAGCTC ACCAGGAGTC TCAAGAGGAA CATCCATCTG 
AAGACCCTGA TGCTGAGACG TGTCTCCCTG GAGAGGGGCG AGCAGTGTCC CCCCGTGCTG 
GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGGA 
AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC 
GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGGGACATTT TCCGTCTCCT 
CTGCAGAGGC TGGTGCTGAG GAATTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA 
TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TTGGCAGTAA CAGCCTGAGG 
GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT 
CTCGAGCTGG AGGAGTGCCA GTTCACTTCC GTCTGCTGCC CAGCCCTGAC CTCCGTGCTC 
CTCCACAACC GGACCCTGCG GCACCTGGAC CTCAGCGGAA ACGGCGTCGG GCTCCGGGGC 
GCCAAGCTCC TGCAGGACGC CGCCCTGAAG AGGGTGCTCC GCCCCGAGGT CGTCCTGTAC 
GTCCCGCAGC TTCCGGTGCG GCAGCCACAG GCCTGGGAGG GAAGGCGCCT TGTCGGGAGG 
GCGACCCTGG GCCCGAGGAA GAAGAAACAA AGCAATGGAG TGGACATGAC GAGAAGACTG 
GAGGGCCCAG TGGTGAACAA GGATGTCCTG AAGATGGTCC AGGACTGGAC AGATGAGTTC 
TGTGATCAGG CCTCAGGGAT GGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006041858.1
CDS	1..3267
Translation	MGDMNLNSDPSSCPSSPLSPFGVSPPLLESSSSSTLPFRNGVMPFMLSVSAEHLQRFKQLLVEENPRPGCTPLTWDQLKSARCGELVHLLTEYFPGQRAWEVARDIFAKMNQTELCLQTQRELNEILPNLEPEALSLRKRELTLEEDESDKIREYKARVMSEHSTLWDRTSWPGNNMDFFYQEPCREDTLLQCLLLPRKPQGRQPKTVVLQGDAGVGKTTIAKKVMQQWAENKFYAHKGWLAFYLHCQDMDRREEQSFSELIACKLSGSLALASRILSHPEHLLLLLDGFEELTLTLIDRQEDLREDWSQKMPGSVLLTSLLSKRMLPDATIIVFLRFSLWKTVSPFLNSPSLITLMGFSAPERSRYFRSYFKNRRDADAALRFAMGNAVLFSMCRVPVICWLVCSCLKQQMERGSSLPRAFPNATAVFIHYLSSLLPTRVRHVIGETPQEQLKRLCSLAVEGMWKNQWVFSETDLNLARLDERDIEVFLGVRVLRRAVAGGDLVAFAHPSFQEFFAALLFVLCFPQRLRNFQVLDHFRILQLLAHPGRRKNHLAGMALFLFGLLNDTCALAVEQLFGCKVSLGNKRKLLKVATMPPDGDPPTPHHGLPQLFYCLHEIREEVFVGQILHDHRKSLLVISKIRDLQVSAFCLKFCRQLRELELTIAWAVAKATSLLPGPLPSPQPEGSERRSLWWQDFCSVFKTHESLEVLTVRDSVMDTEAVETLAAALRHPHCNLRKLIFKRVGSLVVSEGIIRVLVENQYLRHLEIQDTEVGCQVIDALCNTLKHPWCFLQFLRLEGCPFDPTNGAELTRSLKRNIHLKTLMLRRVSLERGEQCPPVLAPQLERLSLENCDLTPLSCGSLAFSLVSNQSLTHLSLAENALQDDGVKQLWNILGHFPSPLQRLVLRNCALTSECCQDIASALDKNKTLRSLDLGSNSLRDSGVVLLCQPLLNPDSGLQVLELEECQFTSVCCPALTSVLLHNRTLRHLDLSGNGVGLRGAKLLQDAALKRVLRPEVVLYVPQLPVRQPQAWEGRRLVGRATLGPRKKKQSNGVDMTRRLEGPVVNKDVLKMVQDWTDEFCDQASGMV

Target ORF information:

RefSeq Version	XM_006041796.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family, pyrin domain containing 8 (NLRP8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006041796.1

ORF Insert Sequence:

ATGGGTGACA TGAACTTGAA CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
TTTGGTGTCT CCCCTCCCTT GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC 
GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAGAGGTT CAAGCAGCTT 
TTGGTGGAGG AGAACCCCAG ACCCGGCTGC ACCCCGCTCA CCTGGGACCA GCTGAAGTCA 
GCCCGCTGCG GGGAGCTGGT TCATCTGCTG ACCGAGTACT TCCCGGGACA ACGTGCCTGG 
GAGGTGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACACAG 
AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG 
GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG 
AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACAT GGACTTCTTT 
TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC 
CAAGGAAGAC AGCCTAAGAC CGTGGTCCTT CAGGGAGACG CCGGCGTCGG GAAAACCACC 
ATAGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG 
CTCGCTTTCT ACCTCCACTG CCAGGATATG GACCGGCGGG AGGAGCAGAG CTTCTCCGAG 
CTGATCGCCT GCAAGTTGTC CGGGTCTCTG GCTCTCGCGT CTAGGATCCT TTCCCACCCA 
GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA 
CAGGAGGACC TGAGAGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT 
CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG 
TGGAAGACCG TGAGCCCCTT TCTGAACAGT CCCTCTCTGA TCACCCTGAT GGGCTTCAGT 
GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA 
GCCTTGCGTT TCGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC 
TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG 
GCATTCCCCA ACGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACCAGG 
GTGAGGCATG TCATCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT 
GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA CGGACCTCAA CCTGGCCAGA 
CTGGACGAAA GGGACATCGA GGTGTTTCTT GGGGTGAGGG TCCTCCGGAG GGCGGTGGCC 
GGTGGGGACC TCGTCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC TGCCCTGCTA 
TTCGTCCTCT GCTTCCCACA GAGGCTCAGG AATTTCCAAG TGTTGGACCA CTTCCGCATC 
TTACAGCTGC TTGCTCATCC TGGGAGAAGG AAAAACCACC TGGCCGGGAT GGCGCTGTTC 
CTGTTTGGCC TGTTGAATGA CACGTGCGCC CTTGCCGTGG AGCAGCTGTT TGGGTGCAAG 
GTGTCCCTGG GGAACAAGAG GAAACTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC 
CCCCCAACCC CACACCACGG GCTCCCCCAG CTGTTCTACT GTCTGCACGA GATCCGGGAG 
GAGGTCTTCG TGGGTCAGAT CCTCCACGAC CACCGTAAGT CTCTGCTGGT CATCAGCAAG 
ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA 
GAACTGACCA TCGCCTGGGC CGTCGCCAAA GCCACCAGCC TCTTGCCAGG CCCTCTTCCT 
TCCCCCCAAC CAGAAGGCAG TGAGAGGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG 
TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACGGAG 
GCCGTGGAGA CTCTTGCCGC AGCACTGAGG CATCCCCACT GTAACCTTCG GAAACTGATC 
TTTAAGCGTG TGGGCTCTCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC 
CAGTACCTGA GACACTTGGA AATACAAGAC ACGGAAGTGG GATGCCAAGT GATAGACGCT 
CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC 
CCATTCGACC CCACCAACGG AGCTGAGCTC ACCAGGAGTC TCAAGAGGAA CATCCATCTG 
AAGACCCTGA TGCTGAGACG TGTCTCCCTG GAGAGGGGCG AGCAGTGTCC CCCCGTGCTG 
GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGGA 
AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC 
GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGGGACATTT TCCGTCTCCT 
CTGCAGAGGC TGGTGCTGAG GAATTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA 
TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TTGGCAGTAA CAGCCTGAGG 
GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT 
CTCGAGCTGG AGGAGTGCCA GTTCACTTCC GTCTGCTGCC CAGCCCTGAC CTCCGTGCTC 
CTCCACAACC GGACCCTGCG GCACCTGGAC CTCAGCGGAA ACGGCGTCGG GCTCCGGGGC 
GCCAAGCTCC TGCAGGACGC CGCCCTGAAG AGGGTGCTCC GCCCCGAGGT CGTCCTGTAC 
GTCCCGCAGC TTCCGGTGCG GCAGCCACAG GCCTGGGAGG GAAGGCGCCT TGTCGGGAGG 
GCGACCCTGG GCCCGAGGAA GAAGAAACAA AGCAATGGAG TGGACATGAC GAGAAGACTG 
GAGGGCCCAG TGGTGAACAA GGATGTCCTG AAGATGGTCC AGGACTGGAC AGATGAGTTC 
TGTGATCAGG CCTCAGGGAT GGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP8 ( XM_025268318.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025124103.1 Bubalus bubalis NLR family pyrin domain containing 8 (NLRP8), mRNA.

Price & Availability↑

CloneID	OBu52062
Clone ID Related Accession (Same CDS sequence)	XM_025268318.1
Accession Version	XM_025268318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037562.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 15% of CDS bases ##RefSeq-Attributes-END##

ATGGCTCTAG AGGTTTACCG CTGGACGCAG CATCTTTGGG CTGAGGACAG GCGTTTTGCC 
CTCTACGAGT CCGGGGGAAC TCCCCAAACC TGTAATTTTT TAAAGTTGCT TGTTCTGCTG 
AATCGAAGAG CTGGTCCCCT CTGCCCGCCG GTGCTGCGGT TCCCAACCCT GAGTGACCAT 
GGAAATGGAG TCATGTCTTT CATGCTTTCT GTAAGCGCGG AGCATCTACA GAGGTTCAAG 
CAGCTTTTGG TGGAGGAGAA CCCCAGACCC GGCTGCACCC CGCTCACCTG GGACCAGCTG 
AAGTCAGCCC GCTGCGGGGA GCTGGTTCAT CTGCTGACCG AGTACTTCCC GGGACAACGT 
GCCTGGGAGG TGGCTCGTGA CATCTTCGCC AAGATGAACC AGACAGAGCT GTGTCTTCAG 
ACACAGAGGG AGCTGAACGA AATTTTACCT AACTTGGAAC CAGAGGCCTT GAGCCTGAGA 
AAGAGGGAGC TGACTTTGGA GGAAGATGAG TCTGATAAAA TTCGGGAGTA CAAGGCACGT 
GTGATGAGTG AGCACTCAAC CCTGTGGGAC AGGACATCTT GGCCTGGGAA CAACATGGAC 
TTCTTTTACC AAGAGCCCTG CAGAGAAGAC ACACTCCTGC AGTGTCTTCT TCTCCCCAGA 
AAGCCCCAAG GAAGACAGCC TAAGACCGTG GTCCTTCAGG GAGACGCCGG CGTCGGGAAA 
ACCACCATAG CCAAAAAAGT GATGCAGCAG TGGGCAGAGA ACAAGTTCTA CGCCCACAAG 
GGCTGGTTCG CTTTCTACCT CCACTGCCAG GATATGGACC AGCCAGAGGA GCAGAGCTTC 
TCCGAGCTGA TCGCCTGCAA GTTGTCCGGG TCTCTGGCTC TCGCGTCTAG GATACTTTCC 
CACCCAGAAC ACCTCCTCCT CCTGCTGGAT GGCTTCGAGG AACTAACCTT GACCCTCATA 
GACAGACAGG AGGACCTGAG AGAAGACTGG AGCCAGAAGA TGCCAGGCTC CGTCCTCCTG 
ACCAGTCTGT TGAGCAAAAG GATGCTCCCT GATGCCACGA TCATCGTCTT CCTGAGGTTC 
AGCTTGTGGA AGACCGTGAG CCCCTTTCTG AACAGTCCCT CTCTGATCAC CCTGATGGGC 
TTCAGTGCGC CTGAGAGATC CAGGTACTTC AGGTCGTACT TCAAAAACAG GCGCGATGCC 
GACGCAGCCT TGCATTTCGC CATGGGTAAC GCGGTCCTCT TCTCCATGTG CCGAGTCCCC 
GTCATCTGCT GGCTGGTCTG CTCTTGTCTG AAGCAACAGA TGGAGAGAGG AAGCAGCCTG 
CCGCGGGCAT TCCCCAACGC CACGGCCGTG TTCATCCACT ACCTCTCCAG CCTGTTGCCA 
ACCAGGGTGA GGCATGTCGT CGGCGAGACC CCCCAGGAGC AGTTGAAACG CCTGTGCTCC 
TTGGCTGTGG AGGGCATGTG GAAGAACCAG TGGGTGTTCA GTGAGACGGA CCTCAACCTG 
GCCAGACTGG ACGAAAGGGA CATTGAGGTG TTTCTTGGGG TGAGGGTCCT CCAGAGGGCG 
GTGGCCAGCG GGGACCTTGT CGCCTTCGCC CACCCGAGCT TCCAGGAGTT CTTTGCCGCC 
CTGCTATTCG TCCTCTGCTT CCCACAGAGG CTCAGGAATT TCCAAGTGTT GGACCACTTC 
CGCATCTTAC AGCTGCTTGC TCATCCTGGG AGAAGGAAAA ACCACCTGGC CGGGATGGCG 
CTGTTCCTGT TTGGCCTGTT GAATGACACG TGCGCCCTCG CCGTGGAGCA GCTGTTTGGG 
TGCAAGGTGT CCCTGGGGAA CAAGAGGAAA CTGCTGAAGG TCGCCACCAT GCCGCCCGAC 
GGTGACCCCC CGACCCCGCA CCACGGGCTC CCCCAGCTGT TCTACTGTCT GCACGAGATC 
CGGGAGGAGG TCTTCGTGGG TCAGATCCTC CACGACCACC GTAAGTCTCT GCTGGTCATC 
AGCAAGATCA GAGACCTGCA GGTCTCCGCT TTCTGCCTCA AGTTCTGCCG ACAGCTGCGG 
GAGTTAGAAC TGACCGTCGC CTGGGCCGTC GCCAAAGCCA CCAGCCTCTT GCCAGCAGCG 
CAGACGCTCC AGCTGTGCCT CCTGGGCTCA GTTTTCTCGA GCTTAATCGC TACTGATGAG 
CCATTTTTTC TGTTTTTGTT TTTGTTTTTT TCACTCCCAC ACAGACCAGA AGGCAGTGAG 
AGGCGTTCTC TCTGGTGGCA AGACTTCTGC TCTGTGTTTA AGACACACGA GAGTTTGGAA 
GTCCTGACTG TGAGAGACAG TGTGATGGAC ACGGAGGCCA TGGAGACTCT TGCCGCAGCA 
CTGAGGCATC CCCACTGTAA CCTCCGGAAA CTGATCTTTA AGCGTGTGGG CTCTCTCGTG 
GTGAGTGAAG GCATCATCAG AGTTTTGGTG GAAAACCAGT ACCTGAGACA CTTGGAAATA 
CAAGACACGG AAGTGGGATG CCAAGTGATA GACGCTCTCT GCAACACCCT GAAACACCCA 
TGGTGTTTTC TGCAGTTTCT CAGGCTGGAA GGTTGCCCAT TCGACCCCAC CAACGGAGCT 
GAGCTCACCA GGAGTCTCAA GAGGAACATC CATCTGAAGA CCCTGATGCT GAGACGTGTC 
TCCCTGGAGA GGGGCGAGCA GTGTCCCCCC GTGCTGGCGC CCCAGCTGGA GAGGCTGTCG 
CTGTTCCTCT TTGGTGCTGA GCGAAGTGGG AGGAGACGTC TGCCTTTAGA TCGGAAGCCC 
AGATGGAAAC CGTTGAGTTT CAGCAGGAGA CCAGGAAAAA CCCAGTCAAG GGAACAAATT 
AAACTGCATC CCGACTGTGA GTGTCTCACC CACAGGCTGG AGAATTGTGA CCTCACGCCG 
CTTTCCTGCG GAAGTCTTGC CTTTTCTCTC GTGAGTAACC AGAGTCTGAC CCACCTGAGC 
CTGGCAGAAA ACGCCTTGCA GGATGACGGG GTGAAGCAGC TCTGGAACAT CTTGGGACAT 
TTTCCGTCTC CTCTGCAGAG GCTGGTGCTG AGGAATTGCG CCCTGACCTC CGAGTGCTGT 
CAAGATATAG CATCTGCTCT TGATAAAAAT AAAACCCTGA GAAGTCTGGA CCTTGGCAGT 
AACAGCCTGA GGGACAGCGG CGTGGTCCTG CTGTGTCAGC CCCTGTTGAA CCCTGACTCT 
GGCCTCCAGG TTCTCGAGCT GGAGGAGTGC CAGTTCACTT CCGTCTGCTG CCCAGCCCTG 
ACCTCCGTGC TCCTCCACAA CCGGACCCTG CGGCACCTGG ACCTCAGCGG AAACGGCGTC 
GGGCTCCGGG GCGCCAAGCT CCTGCAGGAC GCCGCCCTGA AGAGGGTGCT CCGCCCCGAG 
GTCGTCCTGT ACGTCCCCGC AGCTTCCGGT GCGGCAGCCA CAGGCCTGGG AGGGAAGGCG 
CCTTGTCGGG AGGGCGACCC TGGGCCCGAG GTGCTGCTGG CGGAGGCGGC CACATCCTGC 
ACCCCTTTGC TGGGGGTCTA CAGTTCCCTC ATGGGGCTGC CACACCCCAA CTCCTGCTTC 
TGTTCCTTCC TGAGTTCCCT CGGGGCTCAC CAGCTCACCT TTGGCTGTAC CTGCAACCGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025124103.1
CDS	1..3603
Translation	MALEVYRWTQHLWAEDRRFALYESGGTPQTCNFLKLLVLLNRRAGPLCPPVLRFPTLSDHGNGVMSFMLSVSAEHLQRFKQLLVEENPRPGCTPLTWDQLKSARCGELVHLLTEYFPGQRAWEVARDIFAKMNQTELCLQTQRELNEILPNLEPEALSLRKRELTLEEDESDKIREYKARVMSEHSTLWDRTSWPGNNMDFFYQEPCREDTLLQCLLLPRKPQGRQPKTVVLQGDAGVGKTTIAKKVMQQWAENKFYAHKGWFAFYLHCQDMDQPEEQSFSELIACKLSGSLALASRILSHPEHLLLLLDGFEELTLTLIDRQEDLREDWSQKMPGSVLLTSLLSKRMLPDATIIVFLRFSLWKTVSPFLNSPSLITLMGFSAPERSRYFRSYFKNRRDADAALHFAMGNAVLFSMCRVPVICWLVCSCLKQQMERGSSLPRAFPNATAVFIHYLSSLLPTRVRHVVGETPQEQLKRLCSLAVEGMWKNQWVFSETDLNLARLDERDIEVFLGVRVLQRAVASGDLVAFAHPSFQEFFAALLFVLCFPQRLRNFQVLDHFRILQLLAHPGRRKNHLAGMALFLFGLLNDTCALAVEQLFGCKVSLGNKRKLLKVATMPPDGDPPTPHHGLPQLFYCLHEIREEVFVGQILHDHRKSLLVISKIRDLQVSAFCLKFCRQLRELELTVAWAVAKATSLLPAAQTLQLCLLGSVFSSLIATDEPFFLFLFLFFSLPHRPEGSERRSLWWQDFCSVFKTHESLEVLTVRDSVMDTEAMETLAAALRHPHCNLRKLIFKRVGSLVVSEGIIRVLVENQYLRHLEIQDTEVGCQVIDALCNTLKHPWCFLQFLRLEGCPFDPTNGAELTRSLKRNIHLKTLMLRRVSLERGEQCPPVLAPQLERLSLFLFGAERSGRRRLPLDRKPRWKPLSFSRRPGKTQSREQIKLHPDCECLTHRLENCDLTPLSCGSLAFSLVSNQSLTHLSLAENALQDDGVKQLWNILGHFPSPLQRLVLRNCALTSECCQDIASALDKNKTLRSLDLGSNSLRDSGVVLLCQPLLNPDSGLQVLELEECQFTSVCCPALTSVLLHNRTLRHLDLSGNGVGLRGAKLLQDAALKRVLRPEVVLYVPAASGAAATGLGGKAPCREGDPGPEVLLAEAATSCTPLLGVYSSLMGLPHPNSCFCSFLSSLGAHQLTFGCTCNR

Target ORF information:

RefSeq Version	XM_025268318.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family pyrin domain containing 8 (NLRP8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025268318.1

ORF Insert Sequence:

ATGGCTCTAG AGGTTTACCG CTGGACGCAG CATCTTTGGG CTGAGGACAG GCGTTTTGCC 
CTCTACGAGT CCGGGGGAAC TCCCCAAACC TGTAATTTTT TAAAGTTGCT TGTTCTGCTG 
AATCGAAGAG CTGGTCCCCT CTGCCCGCCG GTGCTGCGGT TCCCAACCCT GAGTGACCAT 
GGAAATGGAG TCATGTCTTT CATGCTTTCT GTAAGCGCGG AGCATCTACA GAGGTTCAAG 
CAGCTTTTGG TGGAGGAGAA CCCCAGACCC GGCTGCACCC CGCTCACCTG GGACCAGCTG 
AAGTCAGCCC GCTGCGGGGA GCTGGTTCAT CTGCTGACCG AGTACTTCCC GGGACAACGT 
GCCTGGGAGG TGGCTCGTGA CATCTTCGCC AAGATGAACC AGACAGAGCT GTGTCTTCAG 
ACACAGAGGG AGCTGAACGA AATTTTACCT AACTTGGAAC CAGAGGCCTT GAGCCTGAGA 
AAGAGGGAGC TGACTTTGGA GGAAGATGAG TCTGATAAAA TTCGGGAGTA CAAGGCACGT 
GTGATGAGTG AGCACTCAAC CCTGTGGGAC AGGACATCTT GGCCTGGGAA CAACATGGAC 
TTCTTTTACC AAGAGCCCTG CAGAGAAGAC ACACTCCTGC AGTGTCTTCT TCTCCCCAGA 
AAGCCCCAAG GAAGACAGCC TAAGACCGTG GTCCTTCAGG GAGACGCCGG CGTCGGGAAA 
ACCACCATAG CCAAAAAAGT GATGCAGCAG TGGGCAGAGA ACAAGTTCTA CGCCCACAAG 
GGCTGGTTCG CTTTCTACCT CCACTGCCAG GATATGGACC AGCCAGAGGA GCAGAGCTTC 
TCCGAGCTGA TCGCCTGCAA GTTGTCCGGG TCTCTGGCTC TCGCGTCTAG GATACTTTCC 
CACCCAGAAC ACCTCCTCCT CCTGCTGGAT GGCTTCGAGG AACTAACCTT GACCCTCATA 
GACAGACAGG AGGACCTGAG AGAAGACTGG AGCCAGAAGA TGCCAGGCTC CGTCCTCCTG 
ACCAGTCTGT TGAGCAAAAG GATGCTCCCT GATGCCACGA TCATCGTCTT CCTGAGGTTC 
AGCTTGTGGA AGACCGTGAG CCCCTTTCTG AACAGTCCCT CTCTGATCAC CCTGATGGGC 
TTCAGTGCGC CTGAGAGATC CAGGTACTTC AGGTCGTACT TCAAAAACAG GCGCGATGCC 
GACGCAGCCT TGCATTTCGC CATGGGTAAC GCGGTCCTCT TCTCCATGTG CCGAGTCCCC 
GTCATCTGCT GGCTGGTCTG CTCTTGTCTG AAGCAACAGA TGGAGAGAGG AAGCAGCCTG 
CCGCGGGCAT TCCCCAACGC CACGGCCGTG TTCATCCACT ACCTCTCCAG CCTGTTGCCA 
ACCAGGGTGA GGCATGTCGT CGGCGAGACC CCCCAGGAGC AGTTGAAACG CCTGTGCTCC 
TTGGCTGTGG AGGGCATGTG GAAGAACCAG TGGGTGTTCA GTGAGACGGA CCTCAACCTG 
GCCAGACTGG ACGAAAGGGA CATTGAGGTG TTTCTTGGGG TGAGGGTCCT CCAGAGGGCG 
GTGGCCAGCG GGGACCTTGT CGCCTTCGCC CACCCGAGCT TCCAGGAGTT CTTTGCCGCC 
CTGCTATTCG TCCTCTGCTT CCCACAGAGG CTCAGGAATT TCCAAGTGTT GGACCACTTC 
CGCATCTTAC AGCTGCTTGC TCATCCTGGG AGAAGGAAAA ACCACCTGGC CGGGATGGCG 
CTGTTCCTGT TTGGCCTGTT GAATGACACG TGCGCCCTCG CCGTGGAGCA GCTGTTTGGG 
TGCAAGGTGT CCCTGGGGAA CAAGAGGAAA CTGCTGAAGG TCGCCACCAT GCCGCCCGAC 
GGTGACCCCC CGACCCCGCA CCACGGGCTC CCCCAGCTGT TCTACTGTCT GCACGAGATC 
CGGGAGGAGG TCTTCGTGGG TCAGATCCTC CACGACCACC GTAAGTCTCT GCTGGTCATC 
AGCAAGATCA GAGACCTGCA GGTCTCCGCT TTCTGCCTCA AGTTCTGCCG ACAGCTGCGG 
GAGTTAGAAC TGACCGTCGC CTGGGCCGTC GCCAAAGCCA CCAGCCTCTT GCCAGCAGCG 
CAGACGCTCC AGCTGTGCCT CCTGGGCTCA GTTTTCTCGA GCTTAATCGC TACTGATGAG 
CCATTTTTTC TGTTTTTGTT TTTGTTTTTT TCACTCCCAC ACAGACCAGA AGGCAGTGAG 
AGGCGTTCTC TCTGGTGGCA AGACTTCTGC TCTGTGTTTA AGACACACGA GAGTTTGGAA 
GTCCTGACTG TGAGAGACAG TGTGATGGAC ACGGAGGCCA TGGAGACTCT TGCCGCAGCA 
CTGAGGCATC CCCACTGTAA CCTCCGGAAA CTGATCTTTA AGCGTGTGGG CTCTCTCGTG 
GTGAGTGAAG GCATCATCAG AGTTTTGGTG GAAAACCAGT ACCTGAGACA CTTGGAAATA 
CAAGACACGG AAGTGGGATG CCAAGTGATA GACGCTCTCT GCAACACCCT GAAACACCCA 
TGGTGTTTTC TGCAGTTTCT CAGGCTGGAA GGTTGCCCAT TCGACCCCAC CAACGGAGCT 
GAGCTCACCA GGAGTCTCAA GAGGAACATC CATCTGAAGA CCCTGATGCT GAGACGTGTC 
TCCCTGGAGA GGGGCGAGCA GTGTCCCCCC GTGCTGGCGC CCCAGCTGGA GAGGCTGTCG 
CTGTTCCTCT TTGGTGCTGA GCGAAGTGGG AGGAGACGTC TGCCTTTAGA TCGGAAGCCC 
AGATGGAAAC CGTTGAGTTT CAGCAGGAGA CCAGGAAAAA CCCAGTCAAG GGAACAAATT 
AAACTGCATC CCGACTGTGA GTGTCTCACC CACAGGCTGG AGAATTGTGA CCTCACGCCG 
CTTTCCTGCG GAAGTCTTGC CTTTTCTCTC GTGAGTAACC AGAGTCTGAC CCACCTGAGC 
CTGGCAGAAA ACGCCTTGCA GGATGACGGG GTGAAGCAGC TCTGGAACAT CTTGGGACAT 
TTTCCGTCTC CTCTGCAGAG GCTGGTGCTG AGGAATTGCG CCCTGACCTC CGAGTGCTGT 
CAAGATATAG CATCTGCTCT TGATAAAAAT AAAACCCTGA GAAGTCTGGA CCTTGGCAGT 
AACAGCCTGA GGGACAGCGG CGTGGTCCTG CTGTGTCAGC CCCTGTTGAA CCCTGACTCT 
GGCCTCCAGG TTCTCGAGCT GGAGGAGTGC CAGTTCACTT CCGTCTGCTG CCCAGCCCTG 
ACCTCCGTGC TCCTCCACAA CCGGACCCTG CGGCACCTGG ACCTCAGCGG AAACGGCGTC 
GGGCTCCGGG GCGCCAAGCT CCTGCAGGAC GCCGCCCTGA AGAGGGTGCT CCGCCCCGAG 
GTCGTCCTGT ACGTCCCCGC AGCTTCCGGT GCGGCAGCCA CAGGCCTGGG AGGGAAGGCG 
CCTTGTCGGG AGGGCGACCC TGGGCCCGAG GTGCTGCTGG CGGAGGCGGC CACATCCTGC 
ACCCCTTTGC TGGGGGTCTA CAGTTCCCTC ATGGGGCTGC CACACCCCAA CTCCTGCTTC 
TGTTCCTTCC TGAGTTCCCT CGGGGCTCAC CAGCTCACCT TTGGCTGTAC CTGCAACCGC 
TGA