ubr3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ubr3
Entrez Gene ID	100498471
Full Name	ubiquitin protein ligase E3 component n-recognin 3
Synonyms	znf650
General protein information	Preferred Names ubiquitin protein ligase E3 component n-recognin 3 Names E3 ubiquitin-protein ligase UBR3 ubiquitin protein ligase E3 component n-recognin 3 (putative)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012971082.2	XP_012826536.1	E3 ubiquitin-protein ligase UBR3 isoform X1
XM_012971082.3	XP_012826536.1	E3 ubiquitin-protein ligase UBR3 isoform X1
XM_012971085.2	XP_012826539.1	E3 ubiquitin-protein ligase UBR3 isoform X4
XM_012971083.2	XP_012826537.1	E3 ubiquitin-protein ligase UBR3 isoform X2
XM_012971084.2	XP_012826538.1	E3 ubiquitin-protein ligase UBR3 isoform X3
XM_012971085.3	XP_012826539.1	E3 ubiquitin-protein ligase UBR3 isoform X4
XM_012971083.3	XP_012826537.1	E3 ubiquitin-protein ligase UBR3 isoform X2
XM_012971084.3	XP_012826538.1	E3 ubiquitin-protein ligase UBR3 isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	UBR3	XP_005202447.1
Mus musculus (house mouse)	Ubr3	NP_808451.2
Drosophila melanogaster (fruit fly)	CG42593	NP_001259318.1
	UBR3	XP_001099162.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000384	XP_310723.5
Homo sapiens (human)	UBR3	NP_742067.3
Pan troglodytes (chimpanzee)	UBR3	XP_003309461.1
Canis lupus familiaris (dog)	UBR3	XP_005640460.1
Rattus norvegicus (Norway rat)	Ubr3	XP_006234401.1
Gallus gallus (chicken)	ZNF650	XP_004942807.1
Danio rerio (zebrafish)	ubr3	XP_001919629.4
Xenopus tropicalis (tropical clawed frog)	ubr3	XP_002934668.2

Gene Ontology

The following ubr3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ubr3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa15906	XM_012971082.3 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa37227	XM_012971083.3 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa37228	XM_012971084.3 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa37229	XM_012971085.3 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OXa15906	XM_012971082.2	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa37229	XM_012971085.2	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa37227	XM_012971083.2	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa37228	XM_012971084.2	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ubr3 ( XM_012971082.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826536.1 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X1, mRNA.

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CloneID	OXa15906
Clone ID Related Accession (Same CDS sequence)	XM_012971082.2 , XM_012971082.3
Accession Version	XM_012971082.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971082.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CGGAGACGCC GATAAGCCGG GATAAAGAAG CCACAGCCGC TTTGCTGAAG 
AGCAAACTGA GCCGGCCGGA CCAGAGCCCG GGGTTGGAGC GGCTGAACGG ATTGCTGGAC 
CGGGTTCTGT GCGTAGGAGG GCCGGCCGGG GAGGCCCTGG AGTGGTGCAA GTGCCTGCTG 
GCCGGGGGGG ACAAGTTCGA GGACTTCTGT CAGGCTGTGC GCTCCTATGA CAATGCCACC 
CTGTGCGGCC TGGTCTGGAC GGCGAACTTC GTGGCCTATC GGTGCCGGAC GTGCGGGATA 
TCCCCATGCA TGTCCCTGTG CGCAGAGTGC TTCCACCAGG GCGAACATGC CGGCCATGAC 
TTTAACATGT TCCGCAGCCA GGCCGGGGGG GCTTGTGACT GTGGGGACAG CAATGTCATG 
AGGGAGAGCG GGTTTTGTAA AAGACATAGA GTGAAGCTGA GCTCCTGTGT TCCCTCTGTG 
CCCCAAGACT TGTTGGTGAT GTCTGAATTG GTTATACCTC GTTTTATCTT TTATCTCATT 
CAGTATCTGA GAGATGGGTA TAGTGAACCA GGAGCTGAAG CATCGCCGGA AAAAGATCTA 
CAGAAAACCC TTCAGATGCT GGATCCTCAG ATCTCCTTCT TGGAAGATTT AACCAAGATG 
GGTGGAGCTA TGAGGTCTGT TCTCACCCAG GTCCTGACCA ACCAGCAGCT TTATAAAGAC 
CTTTCTTCTG GTCCTGGAGA GAGTACATGC TCTAAGAGCC ATGAAAAGTA TCTCATAGCT 
CTGAAGGAAT CTGGTTTGTC AATTCCAGAA GAGAATGTTT CCAGTGGGGC ACAGGAGCAG 
TCACCTGGCA CAAGTACACT AGCAGTTAAA GAGGTTGCAG GTGCAGAAGG TGCCCAAGGG 
CAGCTAGAAT CTTCAGATGA GGAGGACCAG GATGGCAGGC AGTGTGTTGG GAAAAGAAAA 
AGAGTGAAGC TAAGCAGTAA CACCAAAGAT CAATCTATCA TGGATATGTT GAAACACAAA 
TGTTTTCTAG AAGAACTGTT ATTTTGGACA ATAAAGTATG AATTTCCACA GAAAATGGTC 
ACTTTCTTAT TGAATATGTT ACCAGATCAA GATTATAAGA TTGCATTTAC GAAAACATTT 
GTCCAGCATT ATGCTTTCAT TATGAAGACG TTGAAGAAAA GCCATGAATC GGACACTATG 
TCCAATCGAA TTGTGCACAT CAGTGTCCAG CTGTTCAGCA ATGAGGAGCT GGCGCGTCAA 
GTCACAGAAG AGTGCCAACT TCTGGACATT ATGGTCACTG TGCTACTGTA CATGATGGAA 
AGTTGCCTTA TTAAAAGTGA ACTGCAAGAT GAAGAAAACA GTTTACATGT GGTGGTGAAT 
TGTGGAGAAG CTTTACTGAA AAACAATACC TACTGGCCCC TTGTTAGTGA TTTTATTAAT 
ATCCTCTCTC ACCAAAGTGT AGCTAAGAAA TTCCTAGAGG ATCACGGGCT ATTAGTTACT 
TGGATGAACT TTGTATCATT CTTTCAAGGT ATGAATTTAA ACAAAAGAGA GCTCACCGAA 
CATGTAGAAT TTGAATCCCA GACATACTAT GCTGCATTTG CTGCTGAACT TGAAGCCTGT 
GCCCAGCCCA TGTGGGGACT TTTATCGCAC TGCAAAGTTC CAGATACACA AGAATACACA 
CGAAATGTTG TTCGATATTG CCTTGAAGCT CTTCAGGATT GGTTTGATGC TATCAATTTT 
GTAGATGAGC CTGCTCCAAA CCAGGTCACA TTTCACTTGC CTCTGCATCG ATACTATGCT 
ATGTTTTTAA GCAAGGCTAT AAAATGCCAA GAAATAGATT TAGATTCTAT TTTGCCTGAC 
CAGGAAATGC TGATGAAACT AATGGTTCAT CCACTACTAA TTCAGGCAAG TTTGTCTGAA 
ATACACAGCA ACATGTGGGT TCGGAATGGT CTCCAGATTA AAGGACAGGC TATGACATAT 
GTGCAGTCAC ATTTTTGCAA TTCCATGATC GACCCAGATA TCTATCTTCT ACAGGTCTGT 
GCTTCAAAAC TTGACCCAGA CTACTTTATT TCATCAGTCT TTGAAAGATT TAAAGTTGTA 
GATCTATTAA CGATGGCATC TCAGCACCAA AACACAGTGC TCGACGCAGA TCATGAACGG 
TCAATGCTTG AGGGAGCTTT AACATTTCTC GTCATTCTTT TAAGCCTCCG CTTGCATTTA 
GGAATGACAG ATGATGAGAT TCTCCGCGCA GAGATGATAT CCCAGCTCTG TATGAATGAC 
CGCACACACA GCTCACTCCT TGACCTTATT CCAGAAAGCC CAAACCCAAA AAGTGGTATT 
ATGCCAGGGA GTTGCAGCTT TGAATCTGTG TTGTCTGCAG TTGCTGATTT TAAAGCTCCC 
GTGTTTGAGC CTGGAGGCTC AATGCAACAA GGAATGTATA CTCCCAAGGG TGACATCTGG 
GATCAGGAAT TTGACCCCGT AATGGTGATT CTGAGGACAG TTTACAGGAG AGATGTACAG 
TCTGCCATGG ACAGATACAC AGCATTTTTA AAGCAGTCTG GGAGATTTTC TGGAAACCCA 
TGGCCGCCTT ACAAGAAAAG GAGCCCTCTG CATCACAGCT ATAAAGGCCT TATGAGGCTA 
TTGCACTGCA AAACATTGCA CATTGTGCTG TTTACACTGC TTTATAAGAT TCTGATGGAT 
CATCAGAATA TGTCAGAACA CGTTCTTTGT ATGGTTTTGT ATCTCATAGA GCTTGGGCTA 
GAAAATTCTC CCGAGGACGA GTCATGTGAA GAGAATTTTG CTGGGCCAGA TAGATGCCAC 
GACAGCTGGT TTCCTGGAAG TAACCTACTG TGCAACATGC GCCACTTTAT TAACTACGTC 
AGAGTGCGAG TACCAGAGAC TGCTCCAGAG GTGAGGAGGG AGCCCCCAGC AAGCACTAGC 
ACTGACGGAC CATCCTCGGC ACAGAATGCA AGACGGCCAT CTCTGCTCCA GAGGGAGAAT 
TCAGGTACAG CTCAGGTTTT CAGCTTGGTA GCAGAACGTC GGAAAAAGTT CCAGGAGATA 
ATTAACCGCA ATAACAGTGA AGCCAATCAA GTTGTGCGCC CCAAATCTTC AGCTCTGAAG 
TGGACGGCAC CCGGTGCAAC TCCTCAGTTA ACGACAGTCA TATTAGAGGT GAAAGAGAGC 
ATGCTATCCC TGCTTATTAA ACTGCACAAG AAGCTCTCGG GAAAGCAGGA TTCCTATTGC 
CCCCCCTGGT TGGATGATCC AGAATGCCTT GTCCAGCCGG ACTTACCTGG GTATTGTCAT 
GGCGATGGTG TAACCGCTGT AGAACGAATT TTACTGAAAG CAGCATCACA AAGTCGTCTG 
AACAGACAGA GTATTGATGA GATATGCAAG AAAGCTCCTC CACCCGTGCC ACCCAAAAAA 
TCTAGTCCTG CTGACAAGAA GACTTTGGGT AAAGAAGAAA GACGGCAGAA AGCGAGGGAG 
AGGCAACAGA AATTGCTTGC GGAATTCGCC TCAAGGCAAA AAAGCTTCAT GGAAACAGCG 
ATGGATGTTG AATCACCTGA AAGTGATGTG GCAATGGAAA TAGCTGGAGC TGAACAGCAC 
GTGGCAGAAC CAATATATGA TTGTGTTATA TGTGGACAGA GTGGCCCATC TACAGAAGAT 
CGCCCTCTAG GGCTTGTTGT TCTGCTTCAG GCATCTTCTG TTTTGGGGCA TCGGCGTAAG 
AGCACAGAGC CAAAGAAACT ACCAACCACA GAGGAGGAGC AGATATACCA AGAGGGCACA 
TGTGCAGCAG CTAATGATGT CAAACTTGCA ATATTACAGA GATATTTCAA AGATAGCTCC 
TGTCTTCAGT CAGTATCAGT TGGTTGGGAA GGCGGCGTCT ATGTTCAGAC CTGTGGCCAT 
ACTCTGCATA TTGACTGCCA TAAATCTTAT ATGGAATCCT TACGGAATGA CCAGGTTTTA 
CAAGGCTTTT CTGTAGACAA AGGGGAATTT ACATGCCCGC TGTGCAGGCA GTTTGCAAAC 
AGCGTCCTTC CATGCTTCCC GGGAGACAGT ACAGAGAGGG GGTCGTGGCA CAGCCATGCA 
GCCAGCAGCA AAAGTCTGCA GGAGCTCATC AAAGAGGTGG AGGATCTTCA GGAGCAGCTG 
GGTACTTTCC CAGTAAGCAT CAGTTCAGAA ACAAATTTAA GCAAGGAAAT GGAGGCCGTT 
ATGAAGGATA TTAAAAGCAC TACGCAGAAG AAATACACAG ACTATAGCAA GACTCAAGGT 
TCCCCAGACA ACGACTTTCT CTTCATGTAT TCAGTGGCAA GAACTAATTT GGAACTGGAG 
CTTATACATT GTGGAGACAA TCTTTGCTCT GGAGGGGCCA GTGCTGCCGG GAAAAGATCA 
TGTCTGAATC AGCTTTTTCA TGTATTAGCT ATGCACATGC GATTATACAG CATTGATCCC 
GAATACAACC CCTGGAAAAA GTTAACCCAA GTCATACAGA AAAAACCTTC CCTCGGAGAC 
GATGAGGAGA GAGAAGAGGT TCCGATACTT TTCCGAGATG TCACCTCTCT TTTGCTCATT 
CAGGTTCTAA CAATGCCCCA GCCATTGTGC AAAGAGCATT TTGTTTGCAT TGTGAAAGTC 
CTCTACACGT TGCTATACAC TCAAGCGATC ACATCCCTCT CGGTAAAACT CCAGGCTGAA 
GATCGCAAGG TTTGGAGGGA ATCTGGATCC AGCAAGAAGA GTTCATATTC TTCTGGAAAA 
CCTTGGGAAG CTTTGCTTGG GTTTGTGATA AGTGAACTAT CAAGAGGGAG CTTATACCAA 
GATGAAGAGA CTGACAGTAT AAACATGAGT GACTTCACAG CTTGGTACGA AGACGCCATT 
GAGCATTATT TACAGCAGTT TTGCTTACCT TTCCTCAGGA TTACAAGCTT TCTCCAGCAT 
CACCTTTTCG GATTAGATTT GCCAAGTTGC CAGGAAGAAG ATGAATTTAC TGTCCTTTCC 
AACTGTTTGG GACTTTTGCC ATCTTTCAGT GAAGCATCGC ACCAGTTTGT CAGTGCCTCC 
TTACTTGACT GGCCAGTGTC CGCATTTGAT TTAATATCTC AGTGGTGTTC AGAAGTCAAT 
TTGTTTTCAG ACAAGCACCC AGACCAAGCT AACGTTTTGC TTATTCAAGA TACCAAGTGG 
GACTTACCAC GCCTCCTTCA GTTGCCAGAA AACTACAATA CAATATTTCA ATATTATCAT 
AGAAAGTGCT GCAGTGTCTG TACAAAGGTT CCCAAGGATC CTGCACTTTG CCTAGTTTGT 
GGCACATTTG TTTGCTTAAA GGGCCTCTGC TGCAAACAAC AGAGTTACTG TGAATGTGTA 
TTGCACTCCC AAAACTGTGG AGCCGGCACT GGAATTTTCC TCTTGATCAA CGCTTCTGTC 
ATCATTATTA TTCGAGGCCA TCGCTTCTGC CTTTGGGGCT CTGTATATCT CGATGCACAT 
GGGGAGGAAG ACAGAGACCT TAGGCGCGGA AAGCCCCTGT ACATCTGTAA GGAAAGGTAC 
AGCATGCTGG AGCAGCAATG GGTCTCGCAC ACGTTTGATC ACATCAATAA GAGATGGGGG 
CCGCATTACA ATGGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826536.1
CDS	171..5771
Translation	MMAAETPISRDKEATAALLKSKLSRPDQSPGLERLNGLLDRVLCVGGPAGEALEWCKCLLAGGDKFEDFCQAVRSYDNATLCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGEHAGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHRVKLSSCVPSVPQDLLVMSELVIPRFIFYLIQYLRDGYSEPGAEASPEKDLQKTLQMLDPQISFLEDLTKMGGAMRSVLTQVLTNQQLYKDLSSGPGESTCSKSHEKYLIALKESGLSIPEENVSSGAQEQSPGTSTLAVKEVAGAEGAQGQLESSDEEDQDGRQCVGKRKRVKLSSNTKDQSIMDMLKHKCFLEELLFWTIKYEFPQKMVTFLLNMLPDQDYKIAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKKFLEDHGLLVTWMNFVSFFQGMNLNKRELTEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVPDTQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAIKCQEIDLDSILPDQEMLMKLMVHPLLIQASLSEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASKLDPDYFISSVFERFKVVDLLTMASQHQNTVLDADHERSMLEGALTFLVILLSLRLHLGMTDDEILRAEMISQLCMNDRTHSSLLDLIPESPNPKSGIMPGSCSFESVLSAVADFKAPVFEPGGSMQQGMYTPKGDIWDQEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGRFSGNPWPPYKKRSPLHHSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNMSEHVLCMVLYLIELGLENSPEDESCEENFAGPDRCHDSWFPGSNLLCNMRHFINYVRVRVPETAPEVRREPPASTSTDGPSSAQNARRPSLLQRENSGTAQVFSLVAERRKKFQEIINRNNSEANQVVRPKSSALKWTAPGATPQLTTVILEVKESMLSLLIKLHKKLSGKQDSYCPPWLDDPECLVQPDLPGYCHGDGVTAVERILLKAASQSRLNRQSIDEICKKAPPPVPPKKSSPADKKTLGKEERRQKARERQQKLLAEFASRQKSFMETAMDVESPESDVAMEIAGAEQHVAEPIYDCVICGQSGPSTEDRPLGLVVLLQASSVLGHRRKSTEPKKLPTTEEEQIYQEGTCAAANDVKLAILQRYFKDSSCLQSVSVGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCFPGDSTERGSWHSHAASSKSLQELIKEVEDLQEQLGTFPVSISSETNLSKEMEAVMKDIKSTTQKKYTDYSKTQGSPDNDFLFMYSVARTNLELELIHCGDNLCSGGASAAGKRSCLNQLFHVLAMHMRLYSIDPEYNPWKKLTQVIQKKPSLGDDEEREEVPILFRDVTSLLLIQVLTMPQPLCKEHFVCIVKVLYTLLYTQAITSLSVKLQAEDRKVWRESGSSKKSSYSSGKPWEALLGFVISELSRGSLYQDEETDSINMSDFTAWYEDAIEHYLQQFCLPFLRITSFLQHHLFGLDLPSCQEEDEFTVLSNCLGLLPSFSEASHQFVSASLLDWPVSAFDLISQWCSEVNLFSDKHPDQANVLLIQDTKWDLPRLLQLPENYNTIFQYYHRKCCSVCTKVPKDPALCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYSMLEQQWVSHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_012971082.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971082.3

ORF Insert Sequence:

ATGATGGCGG CGGAGACGCC GATAAGCCGG GATAAAGAAG CCACAGCCGC TTTGCTGAAG 
AGCAAACTGA GCCGGCCGGA CCAGAGCCCG GGGTTGGAGC GGCTGAACGG ATTGCTGGAC 
CGGGTTCTGT GCGTAGGAGG GCCGGCCGGG GAGGCCCTGG AGTGGTGCAA GTGCCTGCTG 
GCCGGGGGGG ACAAGTTCGA GGACTTCTGT CAGGCTGTGC GCTCCTATGA CAATGCCACC 
CTGTGCGGCC TGGTCTGGAC GGCGAACTTC GTGGCCTATC GGTGCCGGAC GTGCGGGATA 
TCCCCATGCA TGTCCCTGTG CGCAGAGTGC TTCCACCAGG GCGAACATGC CGGCCATGAC 
TTTAACATGT TCCGCAGCCA GGCCGGGGGG GCTTGTGACT GTGGGGACAG CAATGTCATG 
AGGGAGAGCG GGTTTTGTAA AAGACATAGA GTGAAGCTGA GCTCCTGTGT TCCCTCTGTG 
CCCCAAGACT TGTTGGTGAT GTCTGAATTG GTTATACCTC GTTTTATCTT TTATCTCATT 
CAGTATCTGA GAGATGGGTA TAGTGAACCA GGAGCTGAAG CATCGCCGGA AAAAGATCTA 
CAGAAAACCC TTCAGATGCT GGATCCTCAG ATCTCCTTCT TGGAAGATTT AACCAAGATG 
GGTGGAGCTA TGAGGTCTGT TCTCACCCAG GTCCTGACCA ACCAGCAGCT TTATAAAGAC 
CTTTCTTCTG GTCCTGGAGA GAGTACATGC TCTAAGAGCC ATGAAAAGTA TCTCATAGCT 
CTGAAGGAAT CTGGTTTGTC AATTCCAGAA GAGAATGTTT CCAGTGGGGC ACAGGAGCAG 
TCACCTGGCA CAAGTACACT AGCAGTTAAA GAGGTTGCAG GTGCAGAAGG TGCCCAAGGG 
CAGCTAGAAT CTTCAGATGA GGAGGACCAG GATGGCAGGC AGTGTGTTGG GAAAAGAAAA 
AGAGTGAAGC TAAGCAGTAA CACCAAAGAT CAATCTATCA TGGATATGTT GAAACACAAA 
TGTTTTCTAG AAGAACTGTT ATTTTGGACA ATAAAGTATG AATTTCCACA GAAAATGGTC 
ACTTTCTTAT TGAATATGTT ACCAGATCAA GATTATAAGA TTGCATTTAC GAAAACATTT 
GTCCAGCATT ATGCTTTCAT TATGAAGACG TTGAAGAAAA GCCATGAATC GGACACTATG 
TCCAATCGAA TTGTGCACAT CAGTGTCCAG CTGTTCAGCA ATGAGGAGCT GGCGCGTCAA 
GTCACAGAAG AGTGCCAACT TCTGGACATT ATGGTCACTG TGCTACTGTA CATGATGGAA 
AGTTGCCTTA TTAAAAGTGA ACTGCAAGAT GAAGAAAACA GTTTACATGT GGTGGTGAAT 
TGTGGAGAAG CTTTACTGAA AAACAATACC TACTGGCCCC TTGTTAGTGA TTTTATTAAT 
ATCCTCTCTC ACCAAAGTGT AGCTAAGAAA TTCCTAGAGG ATCACGGGCT ATTAGTTACT 
TGGATGAACT TTGTATCATT CTTTCAAGGT ATGAATTTAA ACAAAAGAGA GCTCACCGAA 
CATGTAGAAT TTGAATCCCA GACATACTAT GCTGCATTTG CTGCTGAACT TGAAGCCTGT 
GCCCAGCCCA TGTGGGGACT TTTATCGCAC TGCAAAGTTC CAGATACACA AGAATACACA 
CGAAATGTTG TTCGATATTG CCTTGAAGCT CTTCAGGATT GGTTTGATGC TATCAATTTT 
GTAGATGAGC CTGCTCCAAA CCAGGTCACA TTTCACTTGC CTCTGCATCG ATACTATGCT 
ATGTTTTTAA GCAAGGCTAT AAAATGCCAA GAAATAGATT TAGATTCTAT TTTGCCTGAC 
CAGGAAATGC TGATGAAACT AATGGTTCAT CCACTACTAA TTCAGGCAAG TTTGTCTGAA 
ATACACAGCA ACATGTGGGT TCGGAATGGT CTCCAGATTA AAGGACAGGC TATGACATAT 
GTGCAGTCAC ATTTTTGCAA TTCCATGATC GACCCAGATA TCTATCTTCT ACAGGTCTGT 
GCTTCAAAAC TTGACCCAGA CTACTTTATT TCATCAGTCT TTGAAAGATT TAAAGTTGTA 
GATCTATTAA CGATGGCATC TCAGCACCAA AACACAGTGC TCGACGCAGA TCATGAACGG 
TCAATGCTTG AGGGAGCTTT AACATTTCTC GTCATTCTTT TAAGCCTCCG CTTGCATTTA 
GGAATGACAG ATGATGAGAT TCTCCGCGCA GAGATGATAT CCCAGCTCTG TATGAATGAC 
CGCACACACA GCTCACTCCT TGACCTTATT CCAGAAAGCC CAAACCCAAA AAGTGGTATT 
ATGCCAGGGA GTTGCAGCTT TGAATCTGTG TTGTCTGCAG TTGCTGATTT TAAAGCTCCC 
GTGTTTGAGC CTGGAGGCTC AATGCAACAA GGAATGTATA CTCCCAAGGG TGACATCTGG 
GATCAGGAAT TTGACCCCGT AATGGTGATT CTGAGGACAG TTTACAGGAG AGATGTACAG 
TCTGCCATGG ACAGATACAC AGCATTTTTA AAGCAGTCTG GGAGATTTTC TGGAAACCCA 
TGGCCGCCTT ACAAGAAAAG GAGCCCTCTG CATCACAGCT ATAAAGGCCT TATGAGGCTA 
TTGCACTGCA AAACATTGCA CATTGTGCTG TTTACACTGC TTTATAAGAT TCTGATGGAT 
CATCAGAATA TGTCAGAACA CGTTCTTTGT ATGGTTTTGT ATCTCATAGA GCTTGGGCTA 
GAAAATTCTC CCGAGGACGA GTCATGTGAA GAGAATTTTG CTGGGCCAGA TAGATGCCAC 
GACAGCTGGT TTCCTGGAAG TAACCTACTG TGCAACATGC GCCACTTTAT TAACTACGTC 
AGAGTGCGAG TACCAGAGAC TGCTCCAGAG GTGAGGAGGG AGCCCCCAGC AAGCACTAGC 
ACTGACGGAC CATCCTCGGC ACAGAATGCA AGACGGCCAT CTCTGCTCCA GAGGGAGAAT 
TCAGGTACAG CTCAGGTTTT CAGCTTGGTA GCAGAACGTC GGAAAAAGTT CCAGGAGATA 
ATTAACCGCA ATAACAGTGA AGCCAATCAA GTTGTGCGCC CCAAATCTTC AGCTCTGAAG 
TGGACGGCAC CCGGTGCAAC TCCTCAGTTA ACGACAGTCA TATTAGAGGT GAAAGAGAGC 
ATGCTATCCC TGCTTATTAA ACTGCACAAG AAGCTCTCGG GAAAGCAGGA TTCCTATTGC 
CCCCCCTGGT TGGATGATCC AGAATGCCTT GTCCAGCCGG ACTTACCTGG GTATTGTCAT 
GGCGATGGTG TAACCGCTGT AGAACGAATT TTACTGAAAG CAGCATCACA AAGTCGTCTG 
AACAGACAGA GTATTGATGA GATATGCAAG AAAGCTCCTC CACCCGTGCC ACCCAAAAAA 
TCTAGTCCTG CTGACAAGAA GACTTTGGGT AAAGAAGAAA GACGGCAGAA AGCGAGGGAG 
AGGCAACAGA AATTGCTTGC GGAATTCGCC TCAAGGCAAA AAAGCTTCAT GGAAACAGCG 
ATGGATGTTG AATCACCTGA AAGTGATGTG GCAATGGAAA TAGCTGGAGC TGAACAGCAC 
GTGGCAGAAC CAATATATGA TTGTGTTATA TGTGGACAGA GTGGCCCATC TACAGAAGAT 
CGCCCTCTAG GGCTTGTTGT TCTGCTTCAG GCATCTTCTG TTTTGGGGCA TCGGCGTAAG 
AGCACAGAGC CAAAGAAACT ACCAACCACA GAGGAGGAGC AGATATACCA AGAGGGCACA 
TGTGCAGCAG CTAATGATGT CAAACTTGCA ATATTACAGA GATATTTCAA AGATAGCTCC 
TGTCTTCAGT CAGTATCAGT TGGTTGGGAA GGCGGCGTCT ATGTTCAGAC CTGTGGCCAT 
ACTCTGCATA TTGACTGCCA TAAATCTTAT ATGGAATCCT TACGGAATGA CCAGGTTTTA 
CAAGGCTTTT CTGTAGACAA AGGGGAATTT ACATGCCCGC TGTGCAGGCA GTTTGCAAAC 
AGCGTCCTTC CATGCTTCCC GGGAGACAGT ACAGAGAGGG GGTCGTGGCA CAGCCATGCA 
GCCAGCAGCA AAAGTCTGCA GGAGCTCATC AAAGAGGTGG AGGATCTTCA GGAGCAGCTG 
GGTACTTTCC CAGTAAGCAT CAGTTCAGAA ACAAATTTAA GCAAGGAAAT GGAGGCCGTT 
ATGAAGGATA TTAAAAGCAC TACGCAGAAG AAATACACAG ACTATAGCAA GACTCAAGGT 
TCCCCAGACA ACGACTTTCT CTTCATGTAT TCAGTGGCAA GAACTAATTT GGAACTGGAG 
CTTATACATT GTGGAGACAA TCTTTGCTCT GGAGGGGCCA GTGCTGCCGG GAAAAGATCA 
TGTCTGAATC AGCTTTTTCA TGTATTAGCT ATGCACATGC GATTATACAG CATTGATCCC 
GAATACAACC CCTGGAAAAA GTTAACCCAA GTCATACAGA AAAAACCTTC CCTCGGAGAC 
GATGAGGAGA GAGAAGAGGT TCCGATACTT TTCCGAGATG TCACCTCTCT TTTGCTCATT 
CAGGTTCTAA CAATGCCCCA GCCATTGTGC AAAGAGCATT TTGTTTGCAT TGTGAAAGTC 
CTCTACACGT TGCTATACAC TCAAGCGATC ACATCCCTCT CGGTAAAACT CCAGGCTGAA 
GATCGCAAGG TTTGGAGGGA ATCTGGATCC AGCAAGAAGA GTTCATATTC TTCTGGAAAA 
CCTTGGGAAG CTTTGCTTGG GTTTGTGATA AGTGAACTAT CAAGAGGGAG CTTATACCAA 
GATGAAGAGA CTGACAGTAT AAACATGAGT GACTTCACAG CTTGGTACGA AGACGCCATT 
GAGCATTATT TACAGCAGTT TTGCTTACCT TTCCTCAGGA TTACAAGCTT TCTCCAGCAT 
CACCTTTTCG GATTAGATTT GCCAAGTTGC CAGGAAGAAG ATGAATTTAC TGTCCTTTCC 
AACTGTTTGG GACTTTTGCC ATCTTTCAGT GAAGCATCGC ACCAGTTTGT CAGTGCCTCC 
TTACTTGACT GGCCAGTGTC CGCATTTGAT TTAATATCTC AGTGGTGTTC AGAAGTCAAT 
TTGTTTTCAG ACAAGCACCC AGACCAAGCT AACGTTTTGC TTATTCAAGA TACCAAGTGG 
GACTTACCAC GCCTCCTTCA GTTGCCAGAA AACTACAATA CAATATTTCA ATATTATCAT 
AGAAAGTGCT GCAGTGTCTG TACAAAGGTT CCCAAGGATC CTGCACTTTG CCTAGTTTGT 
GGCACATTTG TTTGCTTAAA GGGCCTCTGC TGCAAACAAC AGAGTTACTG TGAATGTGTA 
TTGCACTCCC AAAACTGTGG AGCCGGCACT GGAATTTTCC TCTTGATCAA CGCTTCTGTC 
ATCATTATTA TTCGAGGCCA TCGCTTCTGC CTTTGGGGCT CTGTATATCT CGATGCACAT 
GGGGAGGAAG ACAGAGACCT TAGGCGCGGA AAGCCCCTGT ACATCTGTAA GGAAAGGTAC 
AGCATGCTGG AGCAGCAATG GGTCTCGCAC ACGTTTGATC ACATCAATAA GAGATGGGGG 
CCGCATTACA ATGGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr3 ( XM_012971083.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826537.1 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X2, mRNA.

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CloneID	OXa37227
Clone ID Related Accession (Same CDS sequence)	XM_012971083.2 , XM_012971083.3
Accession Version	XM_012971083.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971083.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CGGAGACGCC GATAAGCCGG GATAAAGAAG CCACAGCCGC TTTGCTGAAG 
AGCAAACTGA GCCGGCCGGA CCAGAGCCCG GGGTTGGAGC GGCTGAACGG ATTGCTGGAC 
CGGGTTCTGT GCGTAGGAGG GCCGGCCGGG GAGGCCCTGG AGTGGTGCAA GTGCCTGCTG 
GCCGGGGGGG ACAAGTTCGA GGACTTCTGT CAGGCTGTGC GCTCCTATGA CAATGCCACC 
CTGTGCGGCC TGGTCTGGAC GGCGAACTTC GTGGCCTATC GGTGCCGGAC GTGCGGGATA 
TCCCCATGCA TGTCCCTGTG CGCAGAGTGC TTCCACCAGG GCGAACATGC CGGCCATGAC 
TTTAACATGT TCCGCAGCCA GGCCGGGGGG GCTTGTGACT GTGGGGACAG CAATGTCATG 
AGGGAGAGCG GGTTTTGTAA AAGACATAGA GTGAAGCTGA GCTCCTGTGT TCCCTCTGTG 
CCCCAAGACT TGTTGGTGAT GTCTGAATTG GTTATACCTC GTTTTATCTT TTATCTCATT 
CAGTATCTGA GAGATGGGTA TAGTGAACCA GGAGCTGAAG CATCGCCGGA AAAAGATCTA 
CAGAAAACCC TTCAGATGCT GGATCCTCAG ATCTCCTTCT TGGAAGATTT AACCAAGATG 
GGTGGAGCTA TGAGGTCTGT TCTCACCCAG GTCCTGACCA ACCAGCAGCT TTATAAAGAC 
CTTTCTTCTG GTCCTGGAGA GAGTACATGC TCTAAGAGCC ATGAAAAGTA TCTCATAGCT 
CTGAAGGAAT CTGGTTTGTC AATTCCAGAA GAGAATGTTT CCAGTGGGGC ACAGGAGCAG 
TCACCTGGCA CAAGTACACT AGCAGTTAAA GAGGTTGCAG GTGCAGAAGG TGCCCAAGGG 
CAGCTAGAAT CTTCAGATGA GGAGGACCAG GATGGCAGGC AGTGTGTTGG GAAAAGAAAA 
AGAGTGAAGC TAAGCAGTAA CACCAAAGAT CAATCTATCA TGGATATGTT GAAACACAAA 
TGTTTTCTAG AAGAACTGTT ATTTTGGACA ATAAAGTATG AATTTCCACA GAAAATGGTC 
ACTTTCTTAT TGAATATGTT ACCAGATCAA GATTATAAGA TTGCATTTAC GAAAACATTT 
GTCCAGCATT ATGCTTTCAT TATGAAGACG TTGAAGAAAA GCCATGAATC GGACACTATG 
TCCAATCGAA TTGTGCACAT CAGTGTCCAG CTGTTCAGCA ATGAGGAGCT GGCGCGTCAA 
GTCACAGAAG AGTGCCAACT TCTGGACATT ATGGTCACTG TGCTACTGTA CATGATGGAA 
AGTTGCCTTA TTAAAAGTGA ACTGCAAGAT GAAGAAAACA GTTTACATGT GGTGGTGAAT 
TGTGGAGAAG CTTTACTGAA AAACAATACC TACTGGCCCC TTGTTAGTGA TTTTATTAAT 
ATCCTCTCTC ACCAAAGTGT AGCTAAGAAA TTCCTAGAGG ATCACGGGCT ATTAGTTACT 
TGGATGAACT TTGTATCATT CTTTCAAGGT ATGAATTTAA ACAAAAGAGA GCTCACCGAA 
CATGTAGAAT TTGAATCCCA GACATACTAT GCTGCATTTG CTGCTGAACT TGAAGCCTGT 
GCCCAGCCCA TGTGGGGACT TTTATCGCAC TGCAAAGTTC CAGATACACA AGAATACACA 
CGAAATGTTG TTCGATATTG CCTTGAAGCT CTTCAGGATT GGTTTGATGC TATCAATTTT 
GTAGATGAGC CTGCTCCAAA CCAGGTCACA TTTCACTTGC CTCTGCATCG ATACTATGCT 
ATGTTTTTAA GCAAGGCTAT AAAATGCCAA GAAATAGATT TAGATTCTAT TTTGCCTGAC 
CAGGAAATGC TGATGAAACT AATGGTTCAT CCACTACTAA TTCAGGCAAG TTTGTCTGAA 
ATACACAGCA ACATGTGGGT TCGGAATGGT CTCCAGATTA AAGGACAGGC TATGACATAT 
GTGCAGTCAC ATTTTTGCAA TTCCATGATC GACCCAGATA TCTATCTTCT ACAGGTCTGT 
GCTTCAAAAC TTGACCCAGA CTACTTTATT TCATCAGTCT TTGAAAGATT TAAAGTTGTA 
GATCTATTAA CGATGGCATC TCAGCACCAA AACACAGTGC TCGACGCAGA TCATGAACGG 
TCAATGCTTG AGGGAGCTTT AACATTTCTC GTCATTCTTT TAAGCCTCCG CTTGCATTTA 
GGAATGACAG ATGATGAGAT TCTCCGCGCA GAGATGATAT CCCAGCTCTG TATGAATGAC 
CGCACACACA GCTCACTCCT TGACCTTATT CCAGAAAGCC CAAACCCAAA AAGTGGTATT 
ATGCCAGGGA GTTGCAGCTT TGAATCTGTG TTGTCTGCAG TTGCTGATTT TAAAGCTCCC 
GTGTTTGAGC CTGGAGGCTC AATGCAACAA GGAATGTATA CTCCCAAGGG TGACATCTGG 
GATCAGGAAT TTGACCCCGT AATGGTGATT CTGAGGACAG TTTACAGGAG AGATGTACAG 
TCTGCCATGG ACAGATACAC AGCATTTTTA AAGCAGTCTG GGAGATTTTC TGGAAACCCA 
TGGCCGCCTT ACAAGAAAAG GAGCCCTCTG CATCACAGCT ATAAAGGCCT TATGAGGCTA 
TTGCACTGCA AAACATTGCA CATTGTGCTG TTTACACTGC TTTATAAGAT TCTGATGGAT 
CATCAGAATA TGTCAGAACA CGTTCTTTGT ATGGTTTTGT ATCTCATAGA GCTTGGGCTA 
GAAAATTCTC CCGAGGACGA GTCATGTGAA GAGAATTTTG CTGGGCCAGA TAGATGCCAC 
GACAGCTGGT TTCCTGGAAG TAACCTACTG TGCAACATGC GCCACTTTAT TAACTACGTC 
AGAGTGCGAG TACCAGAGAC TGCTCCAGAG GTGAGGAGGG AGCCCCCAGC AAGCACTAGC 
ACTGACGGAC CATCCTCGGC ACAGAATGCA AGACGGCCAT CTCTGCTCCA GAGGGAGAAT 
TCAGGTACAG CTCAGGTTTT CAGCTTGGTA GCAGAACGTC GGAAAAAGTT CCAGGAGATA 
ATTAACCGCA ATAACAGTGA AGCCAATCAA GTTGTGCGCC CCAAATCTTC AGCTCTGAAG 
TGGACGGCAC CCGGTGCAAC TCCTCAGTTA ACGACAGTCA TATTAGAGGT GAAAGAGAGC 
ATGCTATCCC TGCTTATTAA ACTGCACAAG AAGCTCTCGG GAAAGCAGGA TTCCTATTGC 
CCCCCCTGGT TGGATGATCC AGAATGCCTT GTCCAGCCGG ACTTACCTGG GTATTGTCAT 
GGCGATGGTG TAACCGCTGT AGAACGAATT TTACTGAAAG CAGCATCACA AAGTCGTCTG 
AACAGACAGA GTATTGATGA GATATGCAAG AAAGCTCCTC CACCCGTGCC ACCCAAAAAA 
TCTAGTCCTG CTGACAAGAA GACTTTGGGT AAAGAAGAAA GACGGCAGAA AGCGAGGGAG 
AGGCAACAGA AATTGCTTGC GGAATTCGCC TCAAGGCAAA AAAGCTTCAT GGAAACAGCG 
ATGGATGTTG AATCACCTGA AAGTGATGTG GCAATGGAAA TAGCTGGAGC TGAACAGCAC 
GTGGCAGAAC CAATATATGA TTGTGTTATA TGTGGACAGA GTGGCCCATC TACAGAAGAT 
CGCCCTCTAG GGCTTGTTGT TCTGCTTCAG GCATCTTCTG TTTTGGGGCA TCGGCGTAAG 
AGCACAGAGC CAAAGAAACT ACCAACCACA GAGGAGGAGC AGATATACCA AGAGGGCACA 
TGTGCAGCAG CTAATGATGT CAAACTTGCA ATATTACAGA GATATTTCAA AGATAGCTCC 
TGTCTTCAGT CAGTATCAGT TGGTTGGGAA GGCGGCGTCT ATGTTCAGAC CTGTGGCCAT 
ACTCTGCATA TTGACTGCCA TAAATCTTAT ATGGAATCCT TACGGAATGA CCAGGTTTTA 
CAAGGCTTTT CTGTAGACAA AGGGGAATTT ACATGCCCGC TGTGCAGGCA GTTTGCAAAC 
AGCGTCCTTC CATGCTTCCC GGGAGACAGT ACAGAGAGGG GGTCGTGGCA CAGCCATGCA 
GCCAGCAGCA AAAGTCTGCA GGAGCTCATC AAAGAGGTGG AGGATCTTCA GGAGCAGCTG 
GGTACTTTCC CATCAGAAAC AAATTTAAGC AAGGAAATGG AGGCCGTTAT GAAGGATATT 
AAAAGCACTA CGCAGAAGAA ATACACAGAC TATAGCAAGA CTCAAGGTTC CCCAGACAAC 
GACTTTCTCT TCATGTATTC AGTGGCAAGA ACTAATTTGG AACTGGAGCT TATACATTGT 
GGAGACAATC TTTGCTCTGG AGGGGCCAGT GCTGCCGGGA AAAGATCATG TCTGAATCAG 
CTTTTTCATG TATTAGCTAT GCACATGCGA TTATACAGCA TTGATCCCGA ATACAACCCC 
TGGAAAAAGT TAACCCAAGT CATACAGAAA AAACCTTCCC TCGGAGACGA TGAGGAGAGA 
GAAGAGGTTC CGATACTTTT CCGAGATGTC ACCTCTCTTT TGCTCATTCA GGTTCTAACA 
ATGCCCCAGC CATTGTGCAA AGAGCATTTT GTTTGCATTG TGAAAGTCCT CTACACGTTG 
CTATACACTC AAGCGATCAC ATCCCTCTCG GTAAAACTCC AGGCTGAAGA TCGCAAGGTT 
TGGAGGGAAT CTGGATCCAG CAAGAAGAGT TCATATTCTT CTGGAAAACC TTGGGAAGCT 
TTGCTTGGGT TTGTGATAAG TGAACTATCA AGAGGGAGCT TATACCAAGA TGAAGAGACT 
GACAGTATAA ACATGAGTGA CTTCACAGCT TGGTACGAAG ACGCCATTGA GCATTATTTA 
CAGCAGTTTT GCTTACCTTT CCTCAGGATT ACAAGCTTTC TCCAGCATCA CCTTTTCGGA 
TTAGATTTGC CAAGTTGCCA GGAAGAAGAT GAATTTACTG TCCTTTCCAA CTGTTTGGGA 
CTTTTGCCAT CTTTCAGTGA AGCATCGCAC CAGTTTGTCA GTGCCTCCTT ACTTGACTGG 
CCAGTGTCCG CATTTGATTT AATATCTCAG TGGTGTTCAG AAGTCAATTT GTTTTCAGAC 
AAGCACCCAG ACCAAGCTAA CGTTTTGCTT ATTCAAGATA CCAAGTGGGA CTTACCACGC 
CTCCTTCAGT TGCCAGAAAA CTACAATACA ATATTTCAAT ATTATCATAG AAAGTGCTGC 
AGTGTCTGTA CAAAGGTTCC CAAGGATCCT GCACTTTGCC TAGTTTGTGG CACATTTGTT 
TGCTTAAAGG GCCTCTGCTG CAAACAACAG AGTTACTGTG AATGTGTATT GCACTCCCAA 
AACTGTGGAG CCGGCACTGG AATTTTCCTC TTGATCAACG CTTCTGTCAT CATTATTATT 
CGAGGCCATC GCTTCTGCCT TTGGGGCTCT GTATATCTCG ATGCACATGG GGAGGAAGAC 
AGAGACCTTA GGCGCGGAAA GCCCCTGTAC ATCTGTAAGG AAAGGTACAG CATGCTGGAG 
CAGCAATGGG TCTCGCACAC GTTTGATCAC ATCAATAAGA GATGGGGGCC GCATTACAAT 
GGCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826537.1
CDS	171..5759
Translation	MMAAETPISRDKEATAALLKSKLSRPDQSPGLERLNGLLDRVLCVGGPAGEALEWCKCLLAGGDKFEDFCQAVRSYDNATLCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGEHAGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHRVKLSSCVPSVPQDLLVMSELVIPRFIFYLIQYLRDGYSEPGAEASPEKDLQKTLQMLDPQISFLEDLTKMGGAMRSVLTQVLTNQQLYKDLSSGPGESTCSKSHEKYLIALKESGLSIPEENVSSGAQEQSPGTSTLAVKEVAGAEGAQGQLESSDEEDQDGRQCVGKRKRVKLSSNTKDQSIMDMLKHKCFLEELLFWTIKYEFPQKMVTFLLNMLPDQDYKIAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKKFLEDHGLLVTWMNFVSFFQGMNLNKRELTEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVPDTQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAIKCQEIDLDSILPDQEMLMKLMVHPLLIQASLSEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASKLDPDYFISSVFERFKVVDLLTMASQHQNTVLDADHERSMLEGALTFLVILLSLRLHLGMTDDEILRAEMISQLCMNDRTHSSLLDLIPESPNPKSGIMPGSCSFESVLSAVADFKAPVFEPGGSMQQGMYTPKGDIWDQEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGRFSGNPWPPYKKRSPLHHSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNMSEHVLCMVLYLIELGLENSPEDESCEENFAGPDRCHDSWFPGSNLLCNMRHFINYVRVRVPETAPEVRREPPASTSTDGPSSAQNARRPSLLQRENSGTAQVFSLVAERRKKFQEIINRNNSEANQVVRPKSSALKWTAPGATPQLTTVILEVKESMLSLLIKLHKKLSGKQDSYCPPWLDDPECLVQPDLPGYCHGDGVTAVERILLKAASQSRLNRQSIDEICKKAPPPVPPKKSSPADKKTLGKEERRQKARERQQKLLAEFASRQKSFMETAMDVESPESDVAMEIAGAEQHVAEPIYDCVICGQSGPSTEDRPLGLVVLLQASSVLGHRRKSTEPKKLPTTEEEQIYQEGTCAAANDVKLAILQRYFKDSSCLQSVSVGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCFPGDSTERGSWHSHAASSKSLQELIKEVEDLQEQLGTFPSETNLSKEMEAVMKDIKSTTQKKYTDYSKTQGSPDNDFLFMYSVARTNLELELIHCGDNLCSGGASAAGKRSCLNQLFHVLAMHMRLYSIDPEYNPWKKLTQVIQKKPSLGDDEEREEVPILFRDVTSLLLIQVLTMPQPLCKEHFVCIVKVLYTLLYTQAITSLSVKLQAEDRKVWRESGSSKKSSYSSGKPWEALLGFVISELSRGSLYQDEETDSINMSDFTAWYEDAIEHYLQQFCLPFLRITSFLQHHLFGLDLPSCQEEDEFTVLSNCLGLLPSFSEASHQFVSASLLDWPVSAFDLISQWCSEVNLFSDKHPDQANVLLIQDTKWDLPRLLQLPENYNTIFQYYHRKCCSVCTKVPKDPALCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYSMLEQQWVSHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_012971083.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971083.3

ORF Insert Sequence:

ATGATGGCGG CGGAGACGCC GATAAGCCGG GATAAAGAAG CCACAGCCGC TTTGCTGAAG 
AGCAAACTGA GCCGGCCGGA CCAGAGCCCG GGGTTGGAGC GGCTGAACGG ATTGCTGGAC 
CGGGTTCTGT GCGTAGGAGG GCCGGCCGGG GAGGCCCTGG AGTGGTGCAA GTGCCTGCTG 
GCCGGGGGGG ACAAGTTCGA GGACTTCTGT CAGGCTGTGC GCTCCTATGA CAATGCCACC 
CTGTGCGGCC TGGTCTGGAC GGCGAACTTC GTGGCCTATC GGTGCCGGAC GTGCGGGATA 
TCCCCATGCA TGTCCCTGTG CGCAGAGTGC TTCCACCAGG GCGAACATGC CGGCCATGAC 
TTTAACATGT TCCGCAGCCA GGCCGGGGGG GCTTGTGACT GTGGGGACAG CAATGTCATG 
AGGGAGAGCG GGTTTTGTAA AAGACATAGA GTGAAGCTGA GCTCCTGTGT TCCCTCTGTG 
CCCCAAGACT TGTTGGTGAT GTCTGAATTG GTTATACCTC GTTTTATCTT TTATCTCATT 
CAGTATCTGA GAGATGGGTA TAGTGAACCA GGAGCTGAAG CATCGCCGGA AAAAGATCTA 
CAGAAAACCC TTCAGATGCT GGATCCTCAG ATCTCCTTCT TGGAAGATTT AACCAAGATG 
GGTGGAGCTA TGAGGTCTGT TCTCACCCAG GTCCTGACCA ACCAGCAGCT TTATAAAGAC 
CTTTCTTCTG GTCCTGGAGA GAGTACATGC TCTAAGAGCC ATGAAAAGTA TCTCATAGCT 
CTGAAGGAAT CTGGTTTGTC AATTCCAGAA GAGAATGTTT CCAGTGGGGC ACAGGAGCAG 
TCACCTGGCA CAAGTACACT AGCAGTTAAA GAGGTTGCAG GTGCAGAAGG TGCCCAAGGG 
CAGCTAGAAT CTTCAGATGA GGAGGACCAG GATGGCAGGC AGTGTGTTGG GAAAAGAAAA 
AGAGTGAAGC TAAGCAGTAA CACCAAAGAT CAATCTATCA TGGATATGTT GAAACACAAA 
TGTTTTCTAG AAGAACTGTT ATTTTGGACA ATAAAGTATG AATTTCCACA GAAAATGGTC 
ACTTTCTTAT TGAATATGTT ACCAGATCAA GATTATAAGA TTGCATTTAC GAAAACATTT 
GTCCAGCATT ATGCTTTCAT TATGAAGACG TTGAAGAAAA GCCATGAATC GGACACTATG 
TCCAATCGAA TTGTGCACAT CAGTGTCCAG CTGTTCAGCA ATGAGGAGCT GGCGCGTCAA 
GTCACAGAAG AGTGCCAACT TCTGGACATT ATGGTCACTG TGCTACTGTA CATGATGGAA 
AGTTGCCTTA TTAAAAGTGA ACTGCAAGAT GAAGAAAACA GTTTACATGT GGTGGTGAAT 
TGTGGAGAAG CTTTACTGAA AAACAATACC TACTGGCCCC TTGTTAGTGA TTTTATTAAT 
ATCCTCTCTC ACCAAAGTGT AGCTAAGAAA TTCCTAGAGG ATCACGGGCT ATTAGTTACT 
TGGATGAACT TTGTATCATT CTTTCAAGGT ATGAATTTAA ACAAAAGAGA GCTCACCGAA 
CATGTAGAAT TTGAATCCCA GACATACTAT GCTGCATTTG CTGCTGAACT TGAAGCCTGT 
GCCCAGCCCA TGTGGGGACT TTTATCGCAC TGCAAAGTTC CAGATACACA AGAATACACA 
CGAAATGTTG TTCGATATTG CCTTGAAGCT CTTCAGGATT GGTTTGATGC TATCAATTTT 
GTAGATGAGC CTGCTCCAAA CCAGGTCACA TTTCACTTGC CTCTGCATCG ATACTATGCT 
ATGTTTTTAA GCAAGGCTAT AAAATGCCAA GAAATAGATT TAGATTCTAT TTTGCCTGAC 
CAGGAAATGC TGATGAAACT AATGGTTCAT CCACTACTAA TTCAGGCAAG TTTGTCTGAA 
ATACACAGCA ACATGTGGGT TCGGAATGGT CTCCAGATTA AAGGACAGGC TATGACATAT 
GTGCAGTCAC ATTTTTGCAA TTCCATGATC GACCCAGATA TCTATCTTCT ACAGGTCTGT 
GCTTCAAAAC TTGACCCAGA CTACTTTATT TCATCAGTCT TTGAAAGATT TAAAGTTGTA 
GATCTATTAA CGATGGCATC TCAGCACCAA AACACAGTGC TCGACGCAGA TCATGAACGG 
TCAATGCTTG AGGGAGCTTT AACATTTCTC GTCATTCTTT TAAGCCTCCG CTTGCATTTA 
GGAATGACAG ATGATGAGAT TCTCCGCGCA GAGATGATAT CCCAGCTCTG TATGAATGAC 
CGCACACACA GCTCACTCCT TGACCTTATT CCAGAAAGCC CAAACCCAAA AAGTGGTATT 
ATGCCAGGGA GTTGCAGCTT TGAATCTGTG TTGTCTGCAG TTGCTGATTT TAAAGCTCCC 
GTGTTTGAGC CTGGAGGCTC AATGCAACAA GGAATGTATA CTCCCAAGGG TGACATCTGG 
GATCAGGAAT TTGACCCCGT AATGGTGATT CTGAGGACAG TTTACAGGAG AGATGTACAG 
TCTGCCATGG ACAGATACAC AGCATTTTTA AAGCAGTCTG GGAGATTTTC TGGAAACCCA 
TGGCCGCCTT ACAAGAAAAG GAGCCCTCTG CATCACAGCT ATAAAGGCCT TATGAGGCTA 
TTGCACTGCA AAACATTGCA CATTGTGCTG TTTACACTGC TTTATAAGAT TCTGATGGAT 
CATCAGAATA TGTCAGAACA CGTTCTTTGT ATGGTTTTGT ATCTCATAGA GCTTGGGCTA 
GAAAATTCTC CCGAGGACGA GTCATGTGAA GAGAATTTTG CTGGGCCAGA TAGATGCCAC 
GACAGCTGGT TTCCTGGAAG TAACCTACTG TGCAACATGC GCCACTTTAT TAACTACGTC 
AGAGTGCGAG TACCAGAGAC TGCTCCAGAG GTGAGGAGGG AGCCCCCAGC AAGCACTAGC 
ACTGACGGAC CATCCTCGGC ACAGAATGCA AGACGGCCAT CTCTGCTCCA GAGGGAGAAT 
TCAGGTACAG CTCAGGTTTT CAGCTTGGTA GCAGAACGTC GGAAAAAGTT CCAGGAGATA 
ATTAACCGCA ATAACAGTGA AGCCAATCAA GTTGTGCGCC CCAAATCTTC AGCTCTGAAG 
TGGACGGCAC CCGGTGCAAC TCCTCAGTTA ACGACAGTCA TATTAGAGGT GAAAGAGAGC 
ATGCTATCCC TGCTTATTAA ACTGCACAAG AAGCTCTCGG GAAAGCAGGA TTCCTATTGC 
CCCCCCTGGT TGGATGATCC AGAATGCCTT GTCCAGCCGG ACTTACCTGG GTATTGTCAT 
GGCGATGGTG TAACCGCTGT AGAACGAATT TTACTGAAAG CAGCATCACA AAGTCGTCTG 
AACAGACAGA GTATTGATGA GATATGCAAG AAAGCTCCTC CACCCGTGCC ACCCAAAAAA 
TCTAGTCCTG CTGACAAGAA GACTTTGGGT AAAGAAGAAA GACGGCAGAA AGCGAGGGAG 
AGGCAACAGA AATTGCTTGC GGAATTCGCC TCAAGGCAAA AAAGCTTCAT GGAAACAGCG 
ATGGATGTTG AATCACCTGA AAGTGATGTG GCAATGGAAA TAGCTGGAGC TGAACAGCAC 
GTGGCAGAAC CAATATATGA TTGTGTTATA TGTGGACAGA GTGGCCCATC TACAGAAGAT 
CGCCCTCTAG GGCTTGTTGT TCTGCTTCAG GCATCTTCTG TTTTGGGGCA TCGGCGTAAG 
AGCACAGAGC CAAAGAAACT ACCAACCACA GAGGAGGAGC AGATATACCA AGAGGGCACA 
TGTGCAGCAG CTAATGATGT CAAACTTGCA ATATTACAGA GATATTTCAA AGATAGCTCC 
TGTCTTCAGT CAGTATCAGT TGGTTGGGAA GGCGGCGTCT ATGTTCAGAC CTGTGGCCAT 
ACTCTGCATA TTGACTGCCA TAAATCTTAT ATGGAATCCT TACGGAATGA CCAGGTTTTA 
CAAGGCTTTT CTGTAGACAA AGGGGAATTT ACATGCCCGC TGTGCAGGCA GTTTGCAAAC 
AGCGTCCTTC CATGCTTCCC GGGAGACAGT ACAGAGAGGG GGTCGTGGCA CAGCCATGCA 
GCCAGCAGCA AAAGTCTGCA GGAGCTCATC AAAGAGGTGG AGGATCTTCA GGAGCAGCTG 
GGTACTTTCC CATCAGAAAC AAATTTAAGC AAGGAAATGG AGGCCGTTAT GAAGGATATT 
AAAAGCACTA CGCAGAAGAA ATACACAGAC TATAGCAAGA CTCAAGGTTC CCCAGACAAC 
GACTTTCTCT TCATGTATTC AGTGGCAAGA ACTAATTTGG AACTGGAGCT TATACATTGT 
GGAGACAATC TTTGCTCTGG AGGGGCCAGT GCTGCCGGGA AAAGATCATG TCTGAATCAG 
CTTTTTCATG TATTAGCTAT GCACATGCGA TTATACAGCA TTGATCCCGA ATACAACCCC 
TGGAAAAAGT TAACCCAAGT CATACAGAAA AAACCTTCCC TCGGAGACGA TGAGGAGAGA 
GAAGAGGTTC CGATACTTTT CCGAGATGTC ACCTCTCTTT TGCTCATTCA GGTTCTAACA 
ATGCCCCAGC CATTGTGCAA AGAGCATTTT GTTTGCATTG TGAAAGTCCT CTACACGTTG 
CTATACACTC AAGCGATCAC ATCCCTCTCG GTAAAACTCC AGGCTGAAGA TCGCAAGGTT 
TGGAGGGAAT CTGGATCCAG CAAGAAGAGT TCATATTCTT CTGGAAAACC TTGGGAAGCT 
TTGCTTGGGT TTGTGATAAG TGAACTATCA AGAGGGAGCT TATACCAAGA TGAAGAGACT 
GACAGTATAA ACATGAGTGA CTTCACAGCT TGGTACGAAG ACGCCATTGA GCATTATTTA 
CAGCAGTTTT GCTTACCTTT CCTCAGGATT ACAAGCTTTC TCCAGCATCA CCTTTTCGGA 
TTAGATTTGC CAAGTTGCCA GGAAGAAGAT GAATTTACTG TCCTTTCCAA CTGTTTGGGA 
CTTTTGCCAT CTTTCAGTGA AGCATCGCAC CAGTTTGTCA GTGCCTCCTT ACTTGACTGG 
CCAGTGTCCG CATTTGATTT AATATCTCAG TGGTGTTCAG AAGTCAATTT GTTTTCAGAC 
AAGCACCCAG ACCAAGCTAA CGTTTTGCTT ATTCAAGATA CCAAGTGGGA CTTACCACGC 
CTCCTTCAGT TGCCAGAAAA CTACAATACA ATATTTCAAT ATTATCATAG AAAGTGCTGC 
AGTGTCTGTA CAAAGGTTCC CAAGGATCCT GCACTTTGCC TAGTTTGTGG CACATTTGTT 
TGCTTAAAGG GCCTCTGCTG CAAACAACAG AGTTACTGTG AATGTGTATT GCACTCCCAA 
AACTGTGGAG CCGGCACTGG AATTTTCCTC TTGATCAACG CTTCTGTCAT CATTATTATT 
CGAGGCCATC GCTTCTGCCT TTGGGGCTCT GTATATCTCG ATGCACATGG GGAGGAAGAC 
AGAGACCTTA GGCGCGGAAA GCCCCTGTAC ATCTGTAAGG AAAGGTACAG CATGCTGGAG 
CAGCAATGGG TCTCGCACAC GTTTGATCAC ATCAATAAGA GATGGGGGCC GCATTACAAT 
GGCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr3 ( XM_012971084.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826538.1 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X3, mRNA.

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CloneID	OXa37228
Clone ID Related Accession (Same CDS sequence)	XM_012971084.2 , XM_012971084.3
Accession Version	XM_012971084.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971084.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CGGAGACGCC GATAAGCCGG GATAAAGAAG CCACAGCCGC TTTGCTGAAG 
AGCAAACTGA GCCGGCCGGA CCAGAGCCCG GGGTTGGAGC GGCTGAACGG ATTGCTGGAC 
CGGGTTCTGT GCGTAGGAGG GCCGGCCGGG GAGGCCCTGG AGTGGTGCAA GTGCCTGCTG 
GCCGGGGGGG ACAAGTTCGA GGACTTCTGT CAGGCTGTGC GCTCCTATGA CAATGCCACC 
CTGTGCGGCC TGGTCTGGAC GGCGAACTTC GTGGCCTATC GGTGCCGGAC GTGCGGGATA 
TCCCCATGCA TGTCCCTGTG CGCAGAGTGC TTCCACCAGG GCGAACATGC CGGCCATGAC 
TTTAACATGT TCCGCAGCCA GGCCGGGGGG GCTTGTGACT GTGGGGACAG CAATGTCATG 
AGGGAGAGCG GGTTTTGTAA AAGACATAGA GTGAAGCTGA GCTCCTGTGT TCCCTCTGTG 
CCCCAAGACT TGTTGGTGAT GTCTGAATTG GTTATACCTC GTTTTATCTT TTATCTCATT 
CAGTATCTGA GAGATGGGTA TAGTGAACCA GGAGCTGAAG CATCGCCGGA AAAAGATCTA 
CAGAAAACCC TTCAGATGCT GGATCCTCAG ATCTCCTTCT TGGAAGATTT AACCAAGATG 
GGTGGAGCTA TGAGGTCTGT TCTCACCCAG GTCCTGACCA ACCAGCAGCT TTATAAAGAC 
CTTTCTTCTG GTCCTGGAGA GAGTACATGC TCTAAGAGCC ATGAAAAGTA TCTCATAGCT 
CTGAAGGAAT CTGGTTTGTC AATTCCAGAA GAGAATGTTT CCAGTGGGGC ACAGGAGCAG 
TCACCTGGCA CAAGTACACT AGCAGTTAAA GAGGTTGCAG GTGCAGAAGG TGCCCAAGGG 
CAGCTAGAAT CTTCAGATGA GGAGGACCAG GATGGCAGGC AGTGTGTTGG GAAAAGAAAA 
AGAGTGAAGC TAAGCAGTAA CACCAAAGAT CAATCTATCA TGGATATGTT GAAACACAAA 
TGTTTTCTAG AAGAACTGTT ATTTTGGACA ATAAAGTATG AATTTCCACA GAAAATGGTC 
ACTTTCTTAT TGAATATGTT ACCAGATCAA GATTATAAGA TTGCATTTAC GAAAACATTT 
GTCCAGCATT ATGCTTTCAT TATGAAGACG TTGAAGAAAA GCCATGAATC GGACACTATG 
TCCAATCGAA TTGTGCACAT CAGTGTCCAG CTGTTCAGCA ATGAGGAGCT GGCGCGTCAA 
GTCACAGAAG AGTGCCAACT TCTGGACATT ATGGTCACTG TGCTACTGTA CATGATGGAA 
AGTTGCCTTA TTAAAAGTGA ACTGCAAGAT GAAGAAAACA GTTTACATGT GGTGGTGAAT 
TGTGGAGAAG CTTTACTGAA AAACAATACC TACTGGCCCC TTGTTAGTGA TTTTATTAAT 
ATCCTCTCTC ACCAAAGTGT AGCTAAGAAA TTCCTAGAGG ATCACGGGCT ATTAGTTACT 
TGGATGAACT TTGTATCATT CTTTCAAGGT ATGAATTTAA ACAAAAGAGA GCTCACCGAA 
CATGTAGAAT TTGAATCCCA GACATACTAT GCTGCATTTG CTGCTGAACT TGAAGCCTGT 
GCCCAGCCCA TGTGGGGACT TTTATCGCAC TGCAAAGTTC CAGATACACA AGAATACACA 
CGAAATGTTG TTCGATATTG CCTTGAAGCT CTTCAGGATT GGTTTGATGC TATCAATTTT 
GTAGATGAGC CTGCTCCAAA CCAGGTCACA TTTCACTTGC CTCTGCATCG ATACTATGCT 
ATGTTTTTAA GCAAGGCTAT AAAATGCCAA GAAATAGATT TAGATTCTAT TTTGCCTGAC 
CAGGAAATGC TGATGAAACT AATGGTTCAT CCACTACTAA TTCAGGCAAG TTTGTCTGAA 
ATACACAGCA ACATGTGGGT TCGGAATGGT CTCCAGATTA AAGGACAGGC TATGACATAT 
GTGCAGTCAC ATTTTTGCAA TTCCATGATC GACCCAGATA TCTATCTTCT ACAGGTCTGT 
GCTTCAAAAC TTGACCCAGA CTACTTTATT TCATCAGTCT TTGAAAGATT TAAAGTTGTA 
GATCTATTAA CGATGGCATC TCAGCACCAA AACACAGTGC TCGACGCAGA TCATGAACGG 
TCAATGCTTG AGGGAGCTTT AACATTTCTC GTCATTCTTT TAAGCCTCCG CTTGCATTTA 
GGAATGACAG ATGATGAGAT TCTCCGCGCA GAGATGATAT CCCAGCTCTG TATGAATGAC 
CGCACACACA GCTCACTCCT TGACCTTATT CCAGAAAGCC CAAACCCAAA AAGTGGTATT 
ATGCCAGGGA GTTGCAGCTT TGAATCTGTG TTGTCTGCAG TTGCTGATTT TAAAGCTCCC 
GTGTTTGAGC CTGGAGGCTC AATGCAACAA GGAATGTATA CTCCCAAGGG TGACATCTGG 
GATCAGGAAT TTGACCCCGT AATGGTGATT CTGAGGACAG TTTACAGGAG AGATGTACAG 
TCTGCCATGG ACAGATACAC AGCATTTTTA AAGCAGTCTG GGAGATTTTC TGGAAACCCA 
TGGCCGCCTT ACAAGAAAAG GAGCCCTCTG CATCACAGCT ATAAAGGCCT TATGAGGCTA 
TTGCACTGCA AAACATTGCA CATTGTGCTG TTTACACTGC TTTATAAGAT TCTGATGGAT 
CATCAGAATA TGTCAGAACA CGTTCTTTGT ATGGTTTTGT ATCTCATAGA GCTTGGGCTA 
GAAAATTCTC CCGAGGACGA GTCATGTGAA GAGAATTTTG CTGGGCCAGA TAGATGCCAC 
GACAGCTGGT TTCCTGGAAG TAACCTACTG TGCAACATGC GCCACTTTAT TAACTACGTC 
AGAGTGCGAG TACCAGAGAC TGCTCCAGAG GTGAGGAGGG AGCCCCCAGC AAGCACTAGC 
ACTGACGGAC CATCCTCGGC ACAGAATTCA GGTACAGCTC AGGTTTTCAG CTTGGTAGCA 
GAACGTCGGA AAAAGTTCCA GGAGATAATT AACCGCAATA ACAGTGAAGC CAATCAAGTT 
GTGCGCCCCA AATCTTCAGC TCTGAAGTGG ACGGCACCCG GTGCAACTCC TCAGTTAACG 
ACAGTCATAT TAGAGGTGAA AGAGAGCATG CTATCCCTGC TTATTAAACT GCACAAGAAG 
CTCTCGGGAA AGCAGGATTC CTATTGCCCC CCCTGGTTGG ATGATCCAGA ATGCCTTGTC 
CAGCCGGACT TACCTGGGTA TTGTCATGGC GATGGTGTAA CCGCTGTAGA ACGAATTTTA 
CTGAAAGCAG CATCACAAAG TCGTCTGAAC AGACAGAGTA TTGATGAGAT ATGCAAGAAA 
GCTCCTCCAC CCGTGCCACC CAAAAAATCT AGTCCTGCTG ACAAGAAGAC TTTGGGTAAA 
GAAGAAAGAC GGCAGAAAGC GAGGGAGAGG CAACAGAAAT TGCTTGCGGA ATTCGCCTCA 
AGGCAAAAAA GCTTCATGGA AACAGCGATG GATGTTGAAT CACCTGAAAG TGATGTGGCA 
ATGGAAATAG CTGGAGCTGA ACAGCACGTG GCAGAACCAA TATATGATTG TGTTATATGT 
GGACAGAGTG GCCCATCTAC AGAAGATCGC CCTCTAGGGC TTGTTGTTCT GCTTCAGGCA 
TCTTCTGTTT TGGGGCATCG GCGTAAGAGC ACAGAGCCAA AGAAACTACC AACCACAGAG 
GAGGAGCAGA TATACCAAGA GGGCACATGT GCAGCAGCTA ATGATGTCAA ACTTGCAATA 
TTACAGAGAT ATTTCAAAGA TAGCTCCTGT CTTCAGTCAG TATCAGTTGG TTGGGAAGGC 
GGCGTCTATG TTCAGACCTG TGGCCATACT CTGCATATTG ACTGCCATAA ATCTTATATG 
GAATCCTTAC GGAATGACCA GGTTTTACAA GGCTTTTCTG TAGACAAAGG GGAATTTACA 
TGCCCGCTGT GCAGGCAGTT TGCAAACAGC GTCCTTCCAT GCTTCCCGGG AGACAGTACA 
GAGAGGGGGT CGTGGCACAG CCATGCAGCC AGCAGCAAAA GTCTGCAGGA GCTCATCAAA 
GAGGTGGAGG ATCTTCAGGA GCAGCTGGGT ACTTTCCCAG TAAGCATCAG TTCAGAAACA 
AATTTAAGCA AGGAAATGGA GGCCGTTATG AAGGATATTA AAAGCACTAC GCAGAAGAAA 
TACACAGACT ATAGCAAGAC TCAAGGTTCC CCAGACAACG ACTTTCTCTT CATGTATTCA 
GTGGCAAGAA CTAATTTGGA ACTGGAGCTT ATACATTGTG GAGACAATCT TTGCTCTGGA 
GGGGCCAGTG CTGCCGGGAA AAGATCATGT CTGAATCAGC TTTTTCATGT ATTAGCTATG 
CACATGCGAT TATACAGCAT TGATCCCGAA TACAACCCCT GGAAAAAGTT AACCCAAGTC 
ATACAGAAAA AACCTTCCCT CGGAGACGAT GAGGAGAGAG AAGAGGTTCC GATACTTTTC 
CGAGATGTCA CCTCTCTTTT GCTCATTCAG GTTCTAACAA TGCCCCAGCC ATTGTGCAAA 
GAGCATTTTG TTTGCATTGT GAAAGTCCTC TACACGTTGC TATACACTCA AGCGATCACA 
TCCCTCTCGG TAAAACTCCA GGCTGAAGAT CGCAAGGTTT GGAGGGAATC TGGATCCAGC 
AAGAAGAGTT CATATTCTTC TGGAAAACCT TGGGAAGCTT TGCTTGGGTT TGTGATAAGT 
GAACTATCAA GAGGGAGCTT ATACCAAGAT GAAGAGACTG ACAGTATAAA CATGAGTGAC 
TTCACAGCTT GGTACGAAGA CGCCATTGAG CATTATTTAC AGCAGTTTTG CTTACCTTTC 
CTCAGGATTA CAAGCTTTCT CCAGCATCAC CTTTTCGGAT TAGATTTGCC AAGTTGCCAG 
GAAGAAGATG AATTTACTGT CCTTTCCAAC TGTTTGGGAC TTTTGCCATC TTTCAGTGAA 
GCATCGCACC AGTTTGTCAG TGCCTCCTTA CTTGACTGGC CAGTGTCCGC ATTTGATTTA 
ATATCTCAGT GGTGTTCAGA AGTCAATTTG TTTTCAGACA AGCACCCAGA CCAAGCTAAC 
GTTTTGCTTA TTCAAGATAC CAAGTGGGAC TTACCACGCC TCCTTCAGTT GCCAGAAAAC 
TACAATACAA TATTTCAATA TTATCATAGA AAGTGCTGCA GTGTCTGTAC AAAGGTTCCC 
AAGGATCCTG CACTTTGCCT AGTTTGTGGC ACATTTGTTT GCTTAAAGGG CCTCTGCTGC 
AAACAACAGA GTTACTGTGA ATGTGTATTG CACTCCCAAA ACTGTGGAGC CGGCACTGGA 
ATTTTCCTCT TGATCAACGC TTCTGTCATC ATTATTATTC GAGGCCATCG CTTCTGCCTT 
TGGGGCTCTG TATATCTCGA TGCACATGGG GAGGAAGACA GAGACCTTAG GCGCGGAAAG 
CCCCTGTACA TCTGTAAGGA AAGGTACAGC ATGCTGGAGC AGCAATGGGT CTCGCACACG 
TTTGATCACA TCAATAAGAG ATGGGGGCCG CATTACAATG GCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826538.1
CDS	171..5738
Translation	MMAAETPISRDKEATAALLKSKLSRPDQSPGLERLNGLLDRVLCVGGPAGEALEWCKCLLAGGDKFEDFCQAVRSYDNATLCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGEHAGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHRVKLSSCVPSVPQDLLVMSELVIPRFIFYLIQYLRDGYSEPGAEASPEKDLQKTLQMLDPQISFLEDLTKMGGAMRSVLTQVLTNQQLYKDLSSGPGESTCSKSHEKYLIALKESGLSIPEENVSSGAQEQSPGTSTLAVKEVAGAEGAQGQLESSDEEDQDGRQCVGKRKRVKLSSNTKDQSIMDMLKHKCFLEELLFWTIKYEFPQKMVTFLLNMLPDQDYKIAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKKFLEDHGLLVTWMNFVSFFQGMNLNKRELTEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVPDTQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAIKCQEIDLDSILPDQEMLMKLMVHPLLIQASLSEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASKLDPDYFISSVFERFKVVDLLTMASQHQNTVLDADHERSMLEGALTFLVILLSLRLHLGMTDDEILRAEMISQLCMNDRTHSSLLDLIPESPNPKSGIMPGSCSFESVLSAVADFKAPVFEPGGSMQQGMYTPKGDIWDQEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGRFSGNPWPPYKKRSPLHHSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNMSEHVLCMVLYLIELGLENSPEDESCEENFAGPDRCHDSWFPGSNLLCNMRHFINYVRVRVPETAPEVRREPPASTSTDGPSSAQNSGTAQVFSLVAERRKKFQEIINRNNSEANQVVRPKSSALKWTAPGATPQLTTVILEVKESMLSLLIKLHKKLSGKQDSYCPPWLDDPECLVQPDLPGYCHGDGVTAVERILLKAASQSRLNRQSIDEICKKAPPPVPPKKSSPADKKTLGKEERRQKARERQQKLLAEFASRQKSFMETAMDVESPESDVAMEIAGAEQHVAEPIYDCVICGQSGPSTEDRPLGLVVLLQASSVLGHRRKSTEPKKLPTTEEEQIYQEGTCAAANDVKLAILQRYFKDSSCLQSVSVGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCFPGDSTERGSWHSHAASSKSLQELIKEVEDLQEQLGTFPVSISSETNLSKEMEAVMKDIKSTTQKKYTDYSKTQGSPDNDFLFMYSVARTNLELELIHCGDNLCSGGASAAGKRSCLNQLFHVLAMHMRLYSIDPEYNPWKKLTQVIQKKPSLGDDEEREEVPILFRDVTSLLLIQVLTMPQPLCKEHFVCIVKVLYTLLYTQAITSLSVKLQAEDRKVWRESGSSKKSSYSSGKPWEALLGFVISELSRGSLYQDEETDSINMSDFTAWYEDAIEHYLQQFCLPFLRITSFLQHHLFGLDLPSCQEEDEFTVLSNCLGLLPSFSEASHQFVSASLLDWPVSAFDLISQWCSEVNLFSDKHPDQANVLLIQDTKWDLPRLLQLPENYNTIFQYYHRKCCSVCTKVPKDPALCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYSMLEQQWVSHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_012971084.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971084.3

ORF Insert Sequence:

ATGATGGCGG CGGAGACGCC GATAAGCCGG GATAAAGAAG CCACAGCCGC TTTGCTGAAG 
AGCAAACTGA GCCGGCCGGA CCAGAGCCCG GGGTTGGAGC GGCTGAACGG ATTGCTGGAC 
CGGGTTCTGT GCGTAGGAGG GCCGGCCGGG GAGGCCCTGG AGTGGTGCAA GTGCCTGCTG 
GCCGGGGGGG ACAAGTTCGA GGACTTCTGT CAGGCTGTGC GCTCCTATGA CAATGCCACC 
CTGTGCGGCC TGGTCTGGAC GGCGAACTTC GTGGCCTATC GGTGCCGGAC GTGCGGGATA 
TCCCCATGCA TGTCCCTGTG CGCAGAGTGC TTCCACCAGG GCGAACATGC CGGCCATGAC 
TTTAACATGT TCCGCAGCCA GGCCGGGGGG GCTTGTGACT GTGGGGACAG CAATGTCATG 
AGGGAGAGCG GGTTTTGTAA AAGACATAGA GTGAAGCTGA GCTCCTGTGT TCCCTCTGTG 
CCCCAAGACT TGTTGGTGAT GTCTGAATTG GTTATACCTC GTTTTATCTT TTATCTCATT 
CAGTATCTGA GAGATGGGTA TAGTGAACCA GGAGCTGAAG CATCGCCGGA AAAAGATCTA 
CAGAAAACCC TTCAGATGCT GGATCCTCAG ATCTCCTTCT TGGAAGATTT AACCAAGATG 
GGTGGAGCTA TGAGGTCTGT TCTCACCCAG GTCCTGACCA ACCAGCAGCT TTATAAAGAC 
CTTTCTTCTG GTCCTGGAGA GAGTACATGC TCTAAGAGCC ATGAAAAGTA TCTCATAGCT 
CTGAAGGAAT CTGGTTTGTC AATTCCAGAA GAGAATGTTT CCAGTGGGGC ACAGGAGCAG 
TCACCTGGCA CAAGTACACT AGCAGTTAAA GAGGTTGCAG GTGCAGAAGG TGCCCAAGGG 
CAGCTAGAAT CTTCAGATGA GGAGGACCAG GATGGCAGGC AGTGTGTTGG GAAAAGAAAA 
AGAGTGAAGC TAAGCAGTAA CACCAAAGAT CAATCTATCA TGGATATGTT GAAACACAAA 
TGTTTTCTAG AAGAACTGTT ATTTTGGACA ATAAAGTATG AATTTCCACA GAAAATGGTC 
ACTTTCTTAT TGAATATGTT ACCAGATCAA GATTATAAGA TTGCATTTAC GAAAACATTT 
GTCCAGCATT ATGCTTTCAT TATGAAGACG TTGAAGAAAA GCCATGAATC GGACACTATG 
TCCAATCGAA TTGTGCACAT CAGTGTCCAG CTGTTCAGCA ATGAGGAGCT GGCGCGTCAA 
GTCACAGAAG AGTGCCAACT TCTGGACATT ATGGTCACTG TGCTACTGTA CATGATGGAA 
AGTTGCCTTA TTAAAAGTGA ACTGCAAGAT GAAGAAAACA GTTTACATGT GGTGGTGAAT 
TGTGGAGAAG CTTTACTGAA AAACAATACC TACTGGCCCC TTGTTAGTGA TTTTATTAAT 
ATCCTCTCTC ACCAAAGTGT AGCTAAGAAA TTCCTAGAGG ATCACGGGCT ATTAGTTACT 
TGGATGAACT TTGTATCATT CTTTCAAGGT ATGAATTTAA ACAAAAGAGA GCTCACCGAA 
CATGTAGAAT TTGAATCCCA GACATACTAT GCTGCATTTG CTGCTGAACT TGAAGCCTGT 
GCCCAGCCCA TGTGGGGACT TTTATCGCAC TGCAAAGTTC CAGATACACA AGAATACACA 
CGAAATGTTG TTCGATATTG CCTTGAAGCT CTTCAGGATT GGTTTGATGC TATCAATTTT 
GTAGATGAGC CTGCTCCAAA CCAGGTCACA TTTCACTTGC CTCTGCATCG ATACTATGCT 
ATGTTTTTAA GCAAGGCTAT AAAATGCCAA GAAATAGATT TAGATTCTAT TTTGCCTGAC 
CAGGAAATGC TGATGAAACT AATGGTTCAT CCACTACTAA TTCAGGCAAG TTTGTCTGAA 
ATACACAGCA ACATGTGGGT TCGGAATGGT CTCCAGATTA AAGGACAGGC TATGACATAT 
GTGCAGTCAC ATTTTTGCAA TTCCATGATC GACCCAGATA TCTATCTTCT ACAGGTCTGT 
GCTTCAAAAC TTGACCCAGA CTACTTTATT TCATCAGTCT TTGAAAGATT TAAAGTTGTA 
GATCTATTAA CGATGGCATC TCAGCACCAA AACACAGTGC TCGACGCAGA TCATGAACGG 
TCAATGCTTG AGGGAGCTTT AACATTTCTC GTCATTCTTT TAAGCCTCCG CTTGCATTTA 
GGAATGACAG ATGATGAGAT TCTCCGCGCA GAGATGATAT CCCAGCTCTG TATGAATGAC 
CGCACACACA GCTCACTCCT TGACCTTATT CCAGAAAGCC CAAACCCAAA AAGTGGTATT 
ATGCCAGGGA GTTGCAGCTT TGAATCTGTG TTGTCTGCAG TTGCTGATTT TAAAGCTCCC 
GTGTTTGAGC CTGGAGGCTC AATGCAACAA GGAATGTATA CTCCCAAGGG TGACATCTGG 
GATCAGGAAT TTGACCCCGT AATGGTGATT CTGAGGACAG TTTACAGGAG AGATGTACAG 
TCTGCCATGG ACAGATACAC AGCATTTTTA AAGCAGTCTG GGAGATTTTC TGGAAACCCA 
TGGCCGCCTT ACAAGAAAAG GAGCCCTCTG CATCACAGCT ATAAAGGCCT TATGAGGCTA 
TTGCACTGCA AAACATTGCA CATTGTGCTG TTTACACTGC TTTATAAGAT TCTGATGGAT 
CATCAGAATA TGTCAGAACA CGTTCTTTGT ATGGTTTTGT ATCTCATAGA GCTTGGGCTA 
GAAAATTCTC CCGAGGACGA GTCATGTGAA GAGAATTTTG CTGGGCCAGA TAGATGCCAC 
GACAGCTGGT TTCCTGGAAG TAACCTACTG TGCAACATGC GCCACTTTAT TAACTACGTC 
AGAGTGCGAG TACCAGAGAC TGCTCCAGAG GTGAGGAGGG AGCCCCCAGC AAGCACTAGC 
ACTGACGGAC CATCCTCGGC ACAGAATTCA GGTACAGCTC AGGTTTTCAG CTTGGTAGCA 
GAACGTCGGA AAAAGTTCCA GGAGATAATT AACCGCAATA ACAGTGAAGC CAATCAAGTT 
GTGCGCCCCA AATCTTCAGC TCTGAAGTGG ACGGCACCCG GTGCAACTCC TCAGTTAACG 
ACAGTCATAT TAGAGGTGAA AGAGAGCATG CTATCCCTGC TTATTAAACT GCACAAGAAG 
CTCTCGGGAA AGCAGGATTC CTATTGCCCC CCCTGGTTGG ATGATCCAGA ATGCCTTGTC 
CAGCCGGACT TACCTGGGTA TTGTCATGGC GATGGTGTAA CCGCTGTAGA ACGAATTTTA 
CTGAAAGCAG CATCACAAAG TCGTCTGAAC AGACAGAGTA TTGATGAGAT ATGCAAGAAA 
GCTCCTCCAC CCGTGCCACC CAAAAAATCT AGTCCTGCTG ACAAGAAGAC TTTGGGTAAA 
GAAGAAAGAC GGCAGAAAGC GAGGGAGAGG CAACAGAAAT TGCTTGCGGA ATTCGCCTCA 
AGGCAAAAAA GCTTCATGGA AACAGCGATG GATGTTGAAT CACCTGAAAG TGATGTGGCA 
ATGGAAATAG CTGGAGCTGA ACAGCACGTG GCAGAACCAA TATATGATTG TGTTATATGT 
GGACAGAGTG GCCCATCTAC AGAAGATCGC CCTCTAGGGC TTGTTGTTCT GCTTCAGGCA 
TCTTCTGTTT TGGGGCATCG GCGTAAGAGC ACAGAGCCAA AGAAACTACC AACCACAGAG 
GAGGAGCAGA TATACCAAGA GGGCACATGT GCAGCAGCTA ATGATGTCAA ACTTGCAATA 
TTACAGAGAT ATTTCAAAGA TAGCTCCTGT CTTCAGTCAG TATCAGTTGG TTGGGAAGGC 
GGCGTCTATG TTCAGACCTG TGGCCATACT CTGCATATTG ACTGCCATAA ATCTTATATG 
GAATCCTTAC GGAATGACCA GGTTTTACAA GGCTTTTCTG TAGACAAAGG GGAATTTACA 
TGCCCGCTGT GCAGGCAGTT TGCAAACAGC GTCCTTCCAT GCTTCCCGGG AGACAGTACA 
GAGAGGGGGT CGTGGCACAG CCATGCAGCC AGCAGCAAAA GTCTGCAGGA GCTCATCAAA 
GAGGTGGAGG ATCTTCAGGA GCAGCTGGGT ACTTTCCCAG TAAGCATCAG TTCAGAAACA 
AATTTAAGCA AGGAAATGGA GGCCGTTATG AAGGATATTA AAAGCACTAC GCAGAAGAAA 
TACACAGACT ATAGCAAGAC TCAAGGTTCC CCAGACAACG ACTTTCTCTT CATGTATTCA 
GTGGCAAGAA CTAATTTGGA ACTGGAGCTT ATACATTGTG GAGACAATCT TTGCTCTGGA 
GGGGCCAGTG CTGCCGGGAA AAGATCATGT CTGAATCAGC TTTTTCATGT ATTAGCTATG 
CACATGCGAT TATACAGCAT TGATCCCGAA TACAACCCCT GGAAAAAGTT AACCCAAGTC 
ATACAGAAAA AACCTTCCCT CGGAGACGAT GAGGAGAGAG AAGAGGTTCC GATACTTTTC 
CGAGATGTCA CCTCTCTTTT GCTCATTCAG GTTCTAACAA TGCCCCAGCC ATTGTGCAAA 
GAGCATTTTG TTTGCATTGT GAAAGTCCTC TACACGTTGC TATACACTCA AGCGATCACA 
TCCCTCTCGG TAAAACTCCA GGCTGAAGAT CGCAAGGTTT GGAGGGAATC TGGATCCAGC 
AAGAAGAGTT CATATTCTTC TGGAAAACCT TGGGAAGCTT TGCTTGGGTT TGTGATAAGT 
GAACTATCAA GAGGGAGCTT ATACCAAGAT GAAGAGACTG ACAGTATAAA CATGAGTGAC 
TTCACAGCTT GGTACGAAGA CGCCATTGAG CATTATTTAC AGCAGTTTTG CTTACCTTTC 
CTCAGGATTA CAAGCTTTCT CCAGCATCAC CTTTTCGGAT TAGATTTGCC AAGTTGCCAG 
GAAGAAGATG AATTTACTGT CCTTTCCAAC TGTTTGGGAC TTTTGCCATC TTTCAGTGAA 
GCATCGCACC AGTTTGTCAG TGCCTCCTTA CTTGACTGGC CAGTGTCCGC ATTTGATTTA 
ATATCTCAGT GGTGTTCAGA AGTCAATTTG TTTTCAGACA AGCACCCAGA CCAAGCTAAC 
GTTTTGCTTA TTCAAGATAC CAAGTGGGAC TTACCACGCC TCCTTCAGTT GCCAGAAAAC 
TACAATACAA TATTTCAATA TTATCATAGA AAGTGCTGCA GTGTCTGTAC AAAGGTTCCC 
AAGGATCCTG CACTTTGCCT AGTTTGTGGC ACATTTGTTT GCTTAAAGGG CCTCTGCTGC 
AAACAACAGA GTTACTGTGA ATGTGTATTG CACTCCCAAA ACTGTGGAGC CGGCACTGGA 
ATTTTCCTCT TGATCAACGC TTCTGTCATC ATTATTATTC GAGGCCATCG CTTCTGCCTT 
TGGGGCTCTG TATATCTCGA TGCACATGGG GAGGAAGACA GAGACCTTAG GCGCGGAAAG 
CCCCTGTACA TCTGTAAGGA AAGGTACAGC ATGCTGGAGC AGCAATGGGT CTCGCACACG 
TTTGATCACA TCAATAAGAG ATGGGGGCCG CATTACAATG GCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr3 ( XM_012971085.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826539.1 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa37229
Clone ID Related Accession (Same CDS sequence)	XM_012971085.2 , XM_012971085.3
Accession Version	XM_012971085.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971085.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CGGAGACGCC GATAAGCCGG GATAAAGAAG CCACAGCCGC TTTGCTGAAG 
AGCAAACTGA GCCGGCCGGA CCAGAGCCCG GGGTTGGAGC GGCTGAACGG ATTGCTGGAC 
CGGGTTCTGT GCGTAGGAGG GCCGGCCGGG GAGGCCCTGG AGTGGTGCAA GTGCCTGCTG 
GCCGGGGGGG ACAAGTTCGA GGACTTCTGT CAGGCTGTGC GCTCCTATGA CAATGCCACC 
CTGTGCGGCC TGGTCTGGAC GGCGAACTTC GTGGCCTATC GGTGCCGGAC GTGCGGGATA 
TCCCCATGCA TGTCCCTGTG CGCAGAGTGC TTCCACCAGG GCGAACATGC CGGCCATGAC 
TTTAACATGT TCCGCAGCCA GGCCGGGGGG GCTTGTGACT GTGGGGACAG CAATGTCATG 
AGGGAGAGCG GGTTTTGTAA AAGACATAGA GTGAAGCTGA GCTCCTGTGT TCCCTCTGTG 
CCCCAAGACT TGTTGGTGAT GTCTGAATTG GTTATACCTC GTTTTATCTT TTATCTCATT 
CAGTATCTGA GAGATGGGTA TAGTGAACCA GGAGCTGAAG CATCGCCGGA AAAAGATCTA 
CAGAAAACCC TTCAGATGCT GGATCCTCAG ATCTCCTTCT TGGAAGATTT AACCAAGATG 
GGTGGAGCTA TGAGGTCTGT TCTCACCCAG GTCCTGACCA ACCAGCAGCT TTATAAAGAC 
CTTTCTTCTG GTCCTGGAGA GAGTACATGC TCTAAGAGCC ATGAAAAGTA TCTCATAGCT 
CTGAAGGAAT CTGGTTTGTC AATTCCAGAA GAGAATGTTT CCAGTGGGGC ACAGGAGCAG 
TCACCTGGCA CAAGTACACT AGCAGTTAAA GAGGTTGCAG GTGCAGAAGG TGCCCAAGGG 
CAGCTAGAAT CTTCAGATGA GGAGGACCAG GATGGCAGGC AGTGTGTTGG GAAAAGAAAA 
AGAGTGAAGC TAAGCAGTAA CACCAAAGAT CAATCTATCA TGGATATGTT GAAACACAAA 
TGTTTTCTAG AAGAACTGTT ATTTTGGACA ATAAAGTATG AATTTCCACA GAAAATGGTC 
ACTTTCTTAT TGAATATGTT ACCAGATCAA GATTATAAGA TTGCATTTAC GAAAACATTT 
GTCCAGCATT ATGCTTTCAT TATGAAGACG TTGAAGAAAA GCCATGAATC GGACACTATG 
TCCAATCGAA TTGTGCACAT CAGTGTCCAG CTGTTCAGCA ATGAGGAGCT GGCGCGTCAA 
GTCACAGAAG AGTGCCAACT TCTGGACATT ATGGTCACTG TGCTACTGTA CATGATGGAA 
AGTTGCCTTA TTAAAAGTGA ACTGCAAGAT GAAGAAAACA GTTTACATGT GGTGGTGAAT 
TGTGGAGAAG CTTTACTGAA AAACAATACC TACTGGCCCC TTGTTAGTGA TTTTATTAAT 
ATCCTCTCTC ACCAAAGTGT AGCTAAGAAA TTCCTAGAGG ATCACGGGCT ATTAGTTACT 
TGGATGAACT TTGTATCATT CTTTCAAGGT ATGAATTTAA ACAAAAGAGA GCTCACCGAA 
CATGTAGAAT TTGAATCCCA GACATACTAT GCTGCATTTG CTGCTGAACT TGAAGCCTGT 
GCCCAGCCCA TGTGGGGACT TTTATCGCAC TGCAAAGTTC CAGATACACA AGAATACACA 
CGAAATGTTG TTCGATATTG CCTTGAAGCT CTTCAGGATT GGTTTGATGC TATCAATTTT 
GTAGATGAGC CTGCTCCAAA CCAGGTCACA TTTCACTTGC CTCTGCATCG ATACTATGCT 
ATGTTTTTAA GCAAGGCTAT AAAATGCCAA GAAATAGATT TAGATTCTAT TTTGCCTGAC 
CAGGAAATGC TGATGAAACT AATGGTTCAT CCACTACTAA TTCAGGCAAG TTTGTCTGAA 
ATACACAGCA ACATGTGGGT TCGGAATGGT CTCCAGATTA AAGGACAGGC TATGACATAT 
GTGCAGTCAC ATTTTTGCAA TTCCATGATC GACCCAGATA TCTATCTTCT ACAGGTCTGT 
GCTTCAAAAC TTGACCCAGA CTACTTTATT TCATCAGTCT TTGAAAGATT TAAAGTTGTA 
GATCTATTAA CGATGGCATC TCAGCACCAA AACACAGTGC TCGACGCAGA TCATGAACGG 
TCAATGCTTG AGGGAGCTTT AACATTTCTC GTCATTCTTT TAAGCCTCCG CTTGCATTTA 
GGAATGACAG ATGATGAGAT TCTCCGCGCA GAGATGATAT CCCAGCTCTG TATGAATGAC 
CGCACACACA GCTCACTCCT TGACCTTATT CCAGAAAGCC CAAACCCAAA AAGTGGTATT 
ATGCCAGGGA GTTGCAGCTT TGAATCTGTG TTGTCTGCAG TTGCTGATTT TAAAGCTCCC 
GTGTTTGAGC CTGGAGGCTC AATGCAACAA GGAATGTATA CTCCCAAGGG TGACATCTGG 
GATCAGGAAT TTGACCCCGT AATGGTGATT CTGAGGACAG TTTACAGGAG AGATGTACAG 
TCTGCCATGG ACAGATACAC AGCATTTTTA AAGCAGTCTG GGAGATTTTC TGGAAACCCA 
TGGCCGCCTT ACAAGAAAAG GAGCCCTCTG CATCACAGCT ATAAAGGCCT TATGAGGCTA 
TTGCACTGCA AAACATTGCA CATTGTGCTG TTTACACTGC TTTATAAGAT TCTGATGGAT 
CATCAGAATA TGTCAGAACA CGTTCTTTGT ATGGTTTTGT ATCTCATAGA GCTTGGGCTA 
GAAAATTCTC CCGAGGACGA GTCATGTGAA GAGAATTTTG CTGGGCCAGA TAGATGCCAC 
GACAGCTGGT TTCCTGGAAG TAACCTACTG TGCAACATGC GCCACTTTAT TAACTACGTC 
AGAGTGCGAG TACCAGAGAC TGCTCCAGAG GTGAGGAGGG AGCCCCCAGC AAGCACTAGC 
ACTGACGGAC CATCCTCGGC ACAGAATTCA GGTACAGCTC AGGTTTTCAG CTTGGTAGCA 
GAACGTCGGA AAAAGTTCCA GGAGATAATT AACCGCAATA ACAGTGAAGC CAATCAAGTT 
GTGCGCCCCA AATCTTCAGC TCTGAAGTGG ACGGCACCCG GTGCAACTCC TCAGTTAACG 
ACAGTCATAT TAGAGGTGAA AGAGAGCATG CTATCCCTGC TTATTAAACT GCACAAGAAG 
CTCTCGGGAA AGCAGGATTC CTATTGCCCC CCCTGGTTGG ATGATCCAGA ATGCCTTGTC 
CAGCCGGACT TACCTGGGTA TTGTCATGGC GATGGTGTAA CCGCTGTAGA ACGAATTTTA 
CTGAAAGCAG CATCACAAAG TCGTCTGAAC AGACAGAGTA TTGATGAGAT ATGCAAGAAA 
GCTCCTCCAC CCGTGCCACC CAAAAAATCT AGTCCTGCTG ACAAGAAGAC TTTGGGTAAA 
GAAGAAAGAC GGCAGAAAGC GAGGGAGAGG CAACAGAAAT TGCTTGCGGA ATTCGCCTCA 
AGGCAAAAAA GCTTCATGGA AACAGCGATG GATGTTGAAT CACCTGAAAG TGATGTGGCA 
ATGGAAATAG CTGGAGCTGA ACAGCACGTG GCAGAACCAA TATATGATTG TGTTATATGT 
GGACAGAGTG GCCCATCTAC AGAAGATCGC CCTCTAGGGC TTGTTGTTCT GCTTCAGGCA 
TCTTCTGTTT TGGGGCATCG GCGTAAGAGC ACAGAGCCAA AGAAACTACC AACCACAGAG 
GAGGAGCAGA TATACCAAGA GGGCACATGT GCAGCAGCTA ATGATGTCAA ACTTGCAATA 
TTACAGAGAT ATTTCAAAGA TAGCTCCTGT CTTCAGTCAG TATCAGTTGG TTGGGAAGGC 
GGCGTCTATG TTCAGACCTG TGGCCATACT CTGCATATTG ACTGCCATAA ATCTTATATG 
GAATCCTTAC GGAATGACCA GGTTTTACAA GGCTTTTCTG TAGACAAAGG GGAATTTACA 
TGCCCGCTGT GCAGGCAGTT TGCAAACAGC GTCCTTCCAT GCTTCCCGGG AGACAGTACA 
GAGAGGGGGT CGTGGCACAG CCATGCAGCC AGCAGCAAAA GTCTGCAGGA GCTCATCAAA 
GAGGTGGAGG ATCTTCAGGA GCAGCTGGGT ACTTTCCCAT CAGAAACAAA TTTAAGCAAG 
GAAATGGAGG CCGTTATGAA GGATATTAAA AGCACTACGC AGAAGAAATA CACAGACTAT 
AGCAAGACTC AAGGTTCCCC AGACAACGAC TTTCTCTTCA TGTATTCAGT GGCAAGAACT 
AATTTGGAAC TGGAGCTTAT ACATTGTGGA GACAATCTTT GCTCTGGAGG GGCCAGTGCT 
GCCGGGAAAA GATCATGTCT GAATCAGCTT TTTCATGTAT TAGCTATGCA CATGCGATTA 
TACAGCATTG ATCCCGAATA CAACCCCTGG AAAAAGTTAA CCCAAGTCAT ACAGAAAAAA 
CCTTCCCTCG GAGACGATGA GGAGAGAGAA GAGGTTCCGA TACTTTTCCG AGATGTCACC 
TCTCTTTTGC TCATTCAGGT TCTAACAATG CCCCAGCCAT TGTGCAAAGA GCATTTTGTT 
TGCATTGTGA AAGTCCTCTA CACGTTGCTA TACACTCAAG CGATCACATC CCTCTCGGTA 
AAACTCCAGG CTGAAGATCG CAAGGTTTGG AGGGAATCTG GATCCAGCAA GAAGAGTTCA 
TATTCTTCTG GAAAACCTTG GGAAGCTTTG CTTGGGTTTG TGATAAGTGA ACTATCAAGA 
GGGAGCTTAT ACCAAGATGA AGAGACTGAC AGTATAAACA TGAGTGACTT CACAGCTTGG 
TACGAAGACG CCATTGAGCA TTATTTACAG CAGTTTTGCT TACCTTTCCT CAGGATTACA 
AGCTTTCTCC AGCATCACCT TTTCGGATTA GATTTGCCAA GTTGCCAGGA AGAAGATGAA 
TTTACTGTCC TTTCCAACTG TTTGGGACTT TTGCCATCTT TCAGTGAAGC ATCGCACCAG 
TTTGTCAGTG CCTCCTTACT TGACTGGCCA GTGTCCGCAT TTGATTTAAT ATCTCAGTGG 
TGTTCAGAAG TCAATTTGTT TTCAGACAAG CACCCAGACC AAGCTAACGT TTTGCTTATT 
CAAGATACCA AGTGGGACTT ACCACGCCTC CTTCAGTTGC CAGAAAACTA CAATACAATA 
TTTCAATATT ATCATAGAAA GTGCTGCAGT GTCTGTACAA AGGTTCCCAA GGATCCTGCA 
CTTTGCCTAG TTTGTGGCAC ATTTGTTTGC TTAAAGGGCC TCTGCTGCAA ACAACAGAGT 
TACTGTGAAT GTGTATTGCA CTCCCAAAAC TGTGGAGCCG GCACTGGAAT TTTCCTCTTG 
ATCAACGCTT CTGTCATCAT TATTATTCGA GGCCATCGCT TCTGCCTTTG GGGCTCTGTA 
TATCTCGATG CACATGGGGA GGAAGACAGA GACCTTAGGC GCGGAAAGCC CCTGTACATC 
TGTAAGGAAA GGTACAGCAT GCTGGAGCAG CAATGGGTCT CGCACACGTT TGATCACATC 
AATAAGAGAT GGGGGCCGCA TTACAATGGC TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826539.1
CDS	171..5726
Translation	MMAAETPISRDKEATAALLKSKLSRPDQSPGLERLNGLLDRVLCVGGPAGEALEWCKCLLAGGDKFEDFCQAVRSYDNATLCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGEHAGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHRVKLSSCVPSVPQDLLVMSELVIPRFIFYLIQYLRDGYSEPGAEASPEKDLQKTLQMLDPQISFLEDLTKMGGAMRSVLTQVLTNQQLYKDLSSGPGESTCSKSHEKYLIALKESGLSIPEENVSSGAQEQSPGTSTLAVKEVAGAEGAQGQLESSDEEDQDGRQCVGKRKRVKLSSNTKDQSIMDMLKHKCFLEELLFWTIKYEFPQKMVTFLLNMLPDQDYKIAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKKFLEDHGLLVTWMNFVSFFQGMNLNKRELTEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVPDTQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAIKCQEIDLDSILPDQEMLMKLMVHPLLIQASLSEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASKLDPDYFISSVFERFKVVDLLTMASQHQNTVLDADHERSMLEGALTFLVILLSLRLHLGMTDDEILRAEMISQLCMNDRTHSSLLDLIPESPNPKSGIMPGSCSFESVLSAVADFKAPVFEPGGSMQQGMYTPKGDIWDQEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGRFSGNPWPPYKKRSPLHHSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNMSEHVLCMVLYLIELGLENSPEDESCEENFAGPDRCHDSWFPGSNLLCNMRHFINYVRVRVPETAPEVRREPPASTSTDGPSSAQNSGTAQVFSLVAERRKKFQEIINRNNSEANQVVRPKSSALKWTAPGATPQLTTVILEVKESMLSLLIKLHKKLSGKQDSYCPPWLDDPECLVQPDLPGYCHGDGVTAVERILLKAASQSRLNRQSIDEICKKAPPPVPPKKSSPADKKTLGKEERRQKARERQQKLLAEFASRQKSFMETAMDVESPESDVAMEIAGAEQHVAEPIYDCVICGQSGPSTEDRPLGLVVLLQASSVLGHRRKSTEPKKLPTTEEEQIYQEGTCAAANDVKLAILQRYFKDSSCLQSVSVGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCFPGDSTERGSWHSHAASSKSLQELIKEVEDLQEQLGTFPSETNLSKEMEAVMKDIKSTTQKKYTDYSKTQGSPDNDFLFMYSVARTNLELELIHCGDNLCSGGASAAGKRSCLNQLFHVLAMHMRLYSIDPEYNPWKKLTQVIQKKPSLGDDEEREEVPILFRDVTSLLLIQVLTMPQPLCKEHFVCIVKVLYTLLYTQAITSLSVKLQAEDRKVWRESGSSKKSSYSSGKPWEALLGFVISELSRGSLYQDEETDSINMSDFTAWYEDAIEHYLQQFCLPFLRITSFLQHHLFGLDLPSCQEEDEFTVLSNCLGLLPSFSEASHQFVSASLLDWPVSAFDLISQWCSEVNLFSDKHPDQANVLLIQDTKWDLPRLLQLPENYNTIFQYYHRKCCSVCTKVPKDPALCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYSMLEQQWVSHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_012971085.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971085.3

ORF Insert Sequence:

ATGATGGCGG CGGAGACGCC GATAAGCCGG GATAAAGAAG CCACAGCCGC TTTGCTGAAG 
AGCAAACTGA GCCGGCCGGA CCAGAGCCCG GGGTTGGAGC GGCTGAACGG ATTGCTGGAC 
CGGGTTCTGT GCGTAGGAGG GCCGGCCGGG GAGGCCCTGG AGTGGTGCAA GTGCCTGCTG 
GCCGGGGGGG ACAAGTTCGA GGACTTCTGT CAGGCTGTGC GCTCCTATGA CAATGCCACC 
CTGTGCGGCC TGGTCTGGAC GGCGAACTTC GTGGCCTATC GGTGCCGGAC GTGCGGGATA 
TCCCCATGCA TGTCCCTGTG CGCAGAGTGC TTCCACCAGG GCGAACATGC CGGCCATGAC 
TTTAACATGT TCCGCAGCCA GGCCGGGGGG GCTTGTGACT GTGGGGACAG CAATGTCATG 
AGGGAGAGCG GGTTTTGTAA AAGACATAGA GTGAAGCTGA GCTCCTGTGT TCCCTCTGTG 
CCCCAAGACT TGTTGGTGAT GTCTGAATTG GTTATACCTC GTTTTATCTT TTATCTCATT 
CAGTATCTGA GAGATGGGTA TAGTGAACCA GGAGCTGAAG CATCGCCGGA AAAAGATCTA 
CAGAAAACCC TTCAGATGCT GGATCCTCAG ATCTCCTTCT TGGAAGATTT AACCAAGATG 
GGTGGAGCTA TGAGGTCTGT TCTCACCCAG GTCCTGACCA ACCAGCAGCT TTATAAAGAC 
CTTTCTTCTG GTCCTGGAGA GAGTACATGC TCTAAGAGCC ATGAAAAGTA TCTCATAGCT 
CTGAAGGAAT CTGGTTTGTC AATTCCAGAA GAGAATGTTT CCAGTGGGGC ACAGGAGCAG 
TCACCTGGCA CAAGTACACT AGCAGTTAAA GAGGTTGCAG GTGCAGAAGG TGCCCAAGGG 
CAGCTAGAAT CTTCAGATGA GGAGGACCAG GATGGCAGGC AGTGTGTTGG GAAAAGAAAA 
AGAGTGAAGC TAAGCAGTAA CACCAAAGAT CAATCTATCA TGGATATGTT GAAACACAAA 
TGTTTTCTAG AAGAACTGTT ATTTTGGACA ATAAAGTATG AATTTCCACA GAAAATGGTC 
ACTTTCTTAT TGAATATGTT ACCAGATCAA GATTATAAGA TTGCATTTAC GAAAACATTT 
GTCCAGCATT ATGCTTTCAT TATGAAGACG TTGAAGAAAA GCCATGAATC GGACACTATG 
TCCAATCGAA TTGTGCACAT CAGTGTCCAG CTGTTCAGCA ATGAGGAGCT GGCGCGTCAA 
GTCACAGAAG AGTGCCAACT TCTGGACATT ATGGTCACTG TGCTACTGTA CATGATGGAA 
AGTTGCCTTA TTAAAAGTGA ACTGCAAGAT GAAGAAAACA GTTTACATGT GGTGGTGAAT 
TGTGGAGAAG CTTTACTGAA AAACAATACC TACTGGCCCC TTGTTAGTGA TTTTATTAAT 
ATCCTCTCTC ACCAAAGTGT AGCTAAGAAA TTCCTAGAGG ATCACGGGCT ATTAGTTACT 
TGGATGAACT TTGTATCATT CTTTCAAGGT ATGAATTTAA ACAAAAGAGA GCTCACCGAA 
CATGTAGAAT TTGAATCCCA GACATACTAT GCTGCATTTG CTGCTGAACT TGAAGCCTGT 
GCCCAGCCCA TGTGGGGACT TTTATCGCAC TGCAAAGTTC CAGATACACA AGAATACACA 
CGAAATGTTG TTCGATATTG CCTTGAAGCT CTTCAGGATT GGTTTGATGC TATCAATTTT 
GTAGATGAGC CTGCTCCAAA CCAGGTCACA TTTCACTTGC CTCTGCATCG ATACTATGCT 
ATGTTTTTAA GCAAGGCTAT AAAATGCCAA GAAATAGATT TAGATTCTAT TTTGCCTGAC 
CAGGAAATGC TGATGAAACT AATGGTTCAT CCACTACTAA TTCAGGCAAG TTTGTCTGAA 
ATACACAGCA ACATGTGGGT TCGGAATGGT CTCCAGATTA AAGGACAGGC TATGACATAT 
GTGCAGTCAC ATTTTTGCAA TTCCATGATC GACCCAGATA TCTATCTTCT ACAGGTCTGT 
GCTTCAAAAC TTGACCCAGA CTACTTTATT TCATCAGTCT TTGAAAGATT TAAAGTTGTA 
GATCTATTAA CGATGGCATC TCAGCACCAA AACACAGTGC TCGACGCAGA TCATGAACGG 
TCAATGCTTG AGGGAGCTTT AACATTTCTC GTCATTCTTT TAAGCCTCCG CTTGCATTTA 
GGAATGACAG ATGATGAGAT TCTCCGCGCA GAGATGATAT CCCAGCTCTG TATGAATGAC 
CGCACACACA GCTCACTCCT TGACCTTATT CCAGAAAGCC CAAACCCAAA AAGTGGTATT 
ATGCCAGGGA GTTGCAGCTT TGAATCTGTG TTGTCTGCAG TTGCTGATTT TAAAGCTCCC 
GTGTTTGAGC CTGGAGGCTC AATGCAACAA GGAATGTATA CTCCCAAGGG TGACATCTGG 
GATCAGGAAT TTGACCCCGT AATGGTGATT CTGAGGACAG TTTACAGGAG AGATGTACAG 
TCTGCCATGG ACAGATACAC AGCATTTTTA AAGCAGTCTG GGAGATTTTC TGGAAACCCA 
TGGCCGCCTT ACAAGAAAAG GAGCCCTCTG CATCACAGCT ATAAAGGCCT TATGAGGCTA 
TTGCACTGCA AAACATTGCA CATTGTGCTG TTTACACTGC TTTATAAGAT TCTGATGGAT 
CATCAGAATA TGTCAGAACA CGTTCTTTGT ATGGTTTTGT ATCTCATAGA GCTTGGGCTA 
GAAAATTCTC CCGAGGACGA GTCATGTGAA GAGAATTTTG CTGGGCCAGA TAGATGCCAC 
GACAGCTGGT TTCCTGGAAG TAACCTACTG TGCAACATGC GCCACTTTAT TAACTACGTC 
AGAGTGCGAG TACCAGAGAC TGCTCCAGAG GTGAGGAGGG AGCCCCCAGC AAGCACTAGC 
ACTGACGGAC CATCCTCGGC ACAGAATTCA GGTACAGCTC AGGTTTTCAG CTTGGTAGCA 
GAACGTCGGA AAAAGTTCCA GGAGATAATT AACCGCAATA ACAGTGAAGC CAATCAAGTT 
GTGCGCCCCA AATCTTCAGC TCTGAAGTGG ACGGCACCCG GTGCAACTCC TCAGTTAACG 
ACAGTCATAT TAGAGGTGAA AGAGAGCATG CTATCCCTGC TTATTAAACT GCACAAGAAG 
CTCTCGGGAA AGCAGGATTC CTATTGCCCC CCCTGGTTGG ATGATCCAGA ATGCCTTGTC 
CAGCCGGACT TACCTGGGTA TTGTCATGGC GATGGTGTAA CCGCTGTAGA ACGAATTTTA 
CTGAAAGCAG CATCACAAAG TCGTCTGAAC AGACAGAGTA TTGATGAGAT ATGCAAGAAA 
GCTCCTCCAC CCGTGCCACC CAAAAAATCT AGTCCTGCTG ACAAGAAGAC TTTGGGTAAA 
GAAGAAAGAC GGCAGAAAGC GAGGGAGAGG CAACAGAAAT TGCTTGCGGA ATTCGCCTCA 
AGGCAAAAAA GCTTCATGGA AACAGCGATG GATGTTGAAT CACCTGAAAG TGATGTGGCA 
ATGGAAATAG CTGGAGCTGA ACAGCACGTG GCAGAACCAA TATATGATTG TGTTATATGT 
GGACAGAGTG GCCCATCTAC AGAAGATCGC CCTCTAGGGC TTGTTGTTCT GCTTCAGGCA 
TCTTCTGTTT TGGGGCATCG GCGTAAGAGC ACAGAGCCAA AGAAACTACC AACCACAGAG 
GAGGAGCAGA TATACCAAGA GGGCACATGT GCAGCAGCTA ATGATGTCAA ACTTGCAATA 
TTACAGAGAT ATTTCAAAGA TAGCTCCTGT CTTCAGTCAG TATCAGTTGG TTGGGAAGGC 
GGCGTCTATG TTCAGACCTG TGGCCATACT CTGCATATTG ACTGCCATAA ATCTTATATG 
GAATCCTTAC GGAATGACCA GGTTTTACAA GGCTTTTCTG TAGACAAAGG GGAATTTACA 
TGCCCGCTGT GCAGGCAGTT TGCAAACAGC GTCCTTCCAT GCTTCCCGGG AGACAGTACA 
GAGAGGGGGT CGTGGCACAG CCATGCAGCC AGCAGCAAAA GTCTGCAGGA GCTCATCAAA 
GAGGTGGAGG ATCTTCAGGA GCAGCTGGGT ACTTTCCCAT CAGAAACAAA TTTAAGCAAG 
GAAATGGAGG CCGTTATGAA GGATATTAAA AGCACTACGC AGAAGAAATA CACAGACTAT 
AGCAAGACTC AAGGTTCCCC AGACAACGAC TTTCTCTTCA TGTATTCAGT GGCAAGAACT 
AATTTGGAAC TGGAGCTTAT ACATTGTGGA GACAATCTTT GCTCTGGAGG GGCCAGTGCT 
GCCGGGAAAA GATCATGTCT GAATCAGCTT TTTCATGTAT TAGCTATGCA CATGCGATTA 
TACAGCATTG ATCCCGAATA CAACCCCTGG AAAAAGTTAA CCCAAGTCAT ACAGAAAAAA 
CCTTCCCTCG GAGACGATGA GGAGAGAGAA GAGGTTCCGA TACTTTTCCG AGATGTCACC 
TCTCTTTTGC TCATTCAGGT TCTAACAATG CCCCAGCCAT TGTGCAAAGA GCATTTTGTT 
TGCATTGTGA AAGTCCTCTA CACGTTGCTA TACACTCAAG CGATCACATC CCTCTCGGTA 
AAACTCCAGG CTGAAGATCG CAAGGTTTGG AGGGAATCTG GATCCAGCAA GAAGAGTTCA 
TATTCTTCTG GAAAACCTTG GGAAGCTTTG CTTGGGTTTG TGATAAGTGA ACTATCAAGA 
GGGAGCTTAT ACCAAGATGA AGAGACTGAC AGTATAAACA TGAGTGACTT CACAGCTTGG 
TACGAAGACG CCATTGAGCA TTATTTACAG CAGTTTTGCT TACCTTTCCT CAGGATTACA 
AGCTTTCTCC AGCATCACCT TTTCGGATTA GATTTGCCAA GTTGCCAGGA AGAAGATGAA 
TTTACTGTCC TTTCCAACTG TTTGGGACTT TTGCCATCTT TCAGTGAAGC ATCGCACCAG 
TTTGTCAGTG CCTCCTTACT TGACTGGCCA GTGTCCGCAT TTGATTTAAT ATCTCAGTGG 
TGTTCAGAAG TCAATTTGTT TTCAGACAAG CACCCAGACC AAGCTAACGT TTTGCTTATT 
CAAGATACCA AGTGGGACTT ACCACGCCTC CTTCAGTTGC CAGAAAACTA CAATACAATA 
TTTCAATATT ATCATAGAAA GTGCTGCAGT GTCTGTACAA AGGTTCCCAA GGATCCTGCA 
CTTTGCCTAG TTTGTGGCAC ATTTGTTTGC TTAAAGGGCC TCTGCTGCAA ACAACAGAGT 
TACTGTGAAT GTGTATTGCA CTCCCAAAAC TGTGGAGCCG GCACTGGAAT TTTCCTCTTG 
ATCAACGCTT CTGTCATCAT TATTATTCGA GGCCATCGCT TCTGCCTTTG GGGCTCTGTA 
TATCTCGATG CACATGGGGA GGAAGACAGA GACCTTAGGC GCGGAAAGCC CCTGTACATC 
TGTAAGGAAA GGTACAGCAT GCTGGAGCAG CAATGGGTCT CGCACACGTT TGATCACATC 
AATAAGAGAT GGGGGCCGCA TTACAATGGC TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr3 ( XM_012971082.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826536.1 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa15906
Clone ID Related Accession (Same CDS sequence)	XM_012971082.2 , XM_012971082.3
Accession Version	XM_012971082.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CGGAGACGCC GATAAGCCGG GATAAAGAAG CCACAGCCGC TTTGCTGAAG 
AGCAAACTGA GCCGGCCGGA CCAGAGCCCG GGGTTGGAGC GGCTGAACGG ATTGCTGGAC 
CGGGTTCTGT GCGTAGGAGG GCCGGCCGGG GAGGCCCTGG AGTGGTGCAA GTGCCTGCTG 
GCCGGGGGGG ACAAGTTCGA GGACTTCTGT CAGGCTGTGC GCTCCTATGA CAATGCCACC 
CTGTGCGGCC TGGTCTGGAC GGCGAACTTC GTGGCCTATC GGTGCCGGAC GTGCGGGATA 
TCCCCATGCA TGTCCCTGTG CGCAGAGTGC TTCCACCAGG GCGAACATGC CGGCCATGAC 
TTTAACATGT TCCGCAGCCA GGCCGGGGGG GCTTGTGACT GTGGGGACAG CAATGTCATG 
AGGGAGAGCG GGTTTTGTAA AAGACATAGA GTGAAGCTGA GCTCCTGTGT TCCCTCTGTG 
CCCCAAGACT TGTTGGTGAT GTCTGAATTG GTTATACCTC GTTTTATCTT TTATCTCATT 
CAGTATCTGA GAGATGGGTA TAGTGAACCA GGAGCTGAAG CATCGCCGGA AAAAGATCTA 
CAGAAAACCC TTCAGATGCT GGATCCTCAG ATCTCCTTCT TGGAAGATTT AACCAAGATG 
GGTGGAGCTA TGAGGTCTGT TCTCACCCAG GTCCTGACCA ACCAGCAGCT TTATAAAGAC 
CTTTCTTCTG GTCCTGGAGA GAGTACATGC TCTAAGAGCC ATGAAAAGTA TCTCATAGCT 
CTGAAGGAAT CTGGTTTGTC AATTCCAGAA GAGAATGTTT CCAGTGGGGC ACAGGAGCAG 
TCACCTGGCA CAAGTACACT AGCAGTTAAA GAGGTTGCAG GTGCAGAAGG TGCCCAAGGG 
CAGCTAGAAT CTTCAGATGA GGAGGACCAG GATGGCAGGC AGTGTGTTGG GAAAAGAAAA 
AGAGTGAAGC TAAGCAGTAA CACCAAAGAT CAATCTATCA TGGATATGTT GAAACACAAA 
TGTTTTCTAG AAGAACTGTT ATTTTGGACA ATAAAGTATG AATTTCCACA GAAAATGGTC 
ACTTTCTTAT TGAATATGTT ACCAGATCAA GATTATAAGA TTGCATTTAC GAAAACATTT 
GTCCAGCATT ATGCTTTCAT TATGAAGACG TTGAAGAAAA GCCATGAATC GGACACTATG 
TCCAATCGAA TTGTGCACAT CAGTGTCCAG CTGTTCAGCA ATGAGGAGCT GGCGCGTCAA 
GTCACAGAAG AGTGCCAACT TCTGGACATT ATGGTCACTG TGCTACTGTA CATGATGGAA 
AGTTGCCTTA TTAAAAGTGA ACTGCAAGAT GAAGAAAACA GTTTACATGT GGTGGTGAAT 
TGTGGAGAAG CTTTACTGAA AAACAATACC TACTGGCCCC TTGTTAGTGA TTTTATTAAT 
ATCCTCTCTC ACCAAAGTGT AGCTAAGAAA TTCCTAGAGG ATCACGGGCT ATTAGTTACT 
TGGATGAACT TTGTATCATT CTTTCAAGGT ATGAATTTAA ACAAAAGAGA GCTCACCGAA 
CATGTAGAAT TTGAATCCCA GACATACTAT GCTGCATTTG CTGCTGAACT TGAAGCCTGT 
GCCCAGCCCA TGTGGGGACT TTTATCGCAC TGCAAAGTTC CAGATACACA AGAATACACA 
CGAAATGTTG TTCGATATTG CCTTGAAGCT CTTCAGGATT GGTTTGATGC TATCAATTTT 
GTAGATGAGC CTGCTCCAAA CCAGGTCACA TTTCACTTGC CTCTGCATCG ATACTATGCT 
ATGTTTTTAA GCAAGGCTAT AAAATGCCAA GAAATAGATT TAGATTCTAT TTTGCCTGAC 
CAGGAAATGC TGATGAAACT AATGGTTCAT CCACTACTAA TTCAGGCAAG TTTGTCTGAA 
ATACACAGCA ACATGTGGGT TCGGAATGGT CTCCAGATTA AAGGACAGGC TATGACATAT 
GTGCAGTCAC ATTTTTGCAA TTCCATGATC GACCCAGATA TCTATCTTCT ACAGGTCTGT 
GCTTCAAAAC TTGACCCAGA CTACTTTATT TCATCAGTCT TTGAAAGATT TAAAGTTGTA 
GATCTATTAA CGATGGCATC TCAGCACCAA AACACAGTGC TCGACGCAGA TCATGAACGG 
TCAATGCTTG AGGGAGCTTT AACATTTCTC GTCATTCTTT TAAGCCTCCG CTTGCATTTA 
GGAATGACAG ATGATGAGAT TCTCCGCGCA GAGATGATAT CCCAGCTCTG TATGAATGAC 
CGCACACACA GCTCACTCCT TGACCTTATT CCAGAAAGCC CAAACCCAAA AAGTGGTATT 
ATGCCAGGGA GTTGCAGCTT TGAATCTGTG TTGTCTGCAG TTGCTGATTT TAAAGCTCCC 
GTGTTTGAGC CTGGAGGCTC AATGCAACAA GGAATGTATA CTCCCAAGGG TGACATCTGG 
GATCAGGAAT TTGACCCCGT AATGGTGATT CTGAGGACAG TTTACAGGAG AGATGTACAG 
TCTGCCATGG ACAGATACAC AGCATTTTTA AAGCAGTCTG GGAGATTTTC TGGAAACCCA 
TGGCCGCCTT ACAAGAAAAG GAGCCCTCTG CATCACAGCT ATAAAGGCCT TATGAGGCTA 
TTGCACTGCA AAACATTGCA CATTGTGCTG TTTACACTGC TTTATAAGAT TCTGATGGAT 
CATCAGAATA TGTCAGAACA CGTTCTTTGT ATGGTTTTGT ATCTCATAGA GCTTGGGCTA 
GAAAATTCTC CCGAGGACGA GTCATGTGAA GAGAATTTTG CTGGGCCAGA TAGATGCCAC 
GACAGCTGGT TTCCTGGAAG TAACCTACTG TGCAACATGC GCCACTTTAT TAACTACGTC 
AGAGTGCGAG TACCAGAGAC TGCTCCAGAG GTGAGGAGGG AGCCCCCAGC AAGCACTAGC 
ACTGACGGAC CATCCTCGGC ACAGAATGCA AGACGGCCAT CTCTGCTCCA GAGGGAGAAT 
TCAGGTACAG CTCAGGTTTT CAGCTTGGTA GCAGAACGTC GGAAAAAGTT CCAGGAGATA 
ATTAACCGCA ATAACAGTGA AGCCAATCAA GTTGTGCGCC CCAAATCTTC AGCTCTGAAG 
TGGACGGCAC CCGGTGCAAC TCCTCAGTTA ACGACAGTCA TATTAGAGGT GAAAGAGAGC 
ATGCTATCCC TGCTTATTAA ACTGCACAAG AAGCTCTCGG GAAAGCAGGA TTCCTATTGC 
CCCCCCTGGT TGGATGATCC AGAATGCCTT GTCCAGCCGG ACTTACCTGG GTATTGTCAT 
GGCGATGGTG TAACCGCTGT AGAACGAATT TTACTGAAAG CAGCATCACA AAGTCGTCTG 
AACAGACAGA GTATTGATGA GATATGCAAG AAAGCTCCTC CACCCGTGCC ACCCAAAAAA 
TCTAGTCCTG CTGACAAGAA GACTTTGGGT AAAGAAGAAA GACGGCAGAA AGCGAGGGAG 
AGGCAACAGA AATTGCTTGC GGAATTCGCC TCAAGGCAAA AAAGCTTCAT GGAAACAGCG 
ATGGATGTTG AATCACCTGA AAGTGATGTG GCAATGGAAA TAGCTGGAGC TGAACAGCAC 
GTGGCAGAAC CAATATATGA TTGTGTTATA TGTGGACAGA GTGGCCCATC TACAGAAGAT 
CGCCCTCTAG GGCTTGTTGT TCTGCTTCAG GCATCTTCTG TTTTGGGGCA TCGGCGTAAG 
AGCACAGAGC CAAAGAAACT ACCAACCACA GAGGAGGAGC AGATATACCA AGAGGGCACA 
TGTGCAGCAG CTAATGATGT CAAACTTGCA ATATTACAGA GATATTTCAA AGATAGCTCC 
TGTCTTCAGT CAGTATCAGT TGGTTGGGAA GGCGGCGTCT ATGTTCAGAC CTGTGGCCAT 
ACTCTGCATA TTGACTGCCA TAAATCTTAT ATGGAATCCT TACGGAATGA CCAGGTTTTA 
CAAGGCTTTT CTGTAGACAA AGGGGAATTT ACATGCCCGC TGTGCAGGCA GTTTGCAAAC 
AGCGTCCTTC CATGCTTCCC GGGAGACAGT ACAGAGAGGG GGTCGTGGCA CAGCCATGCA 
GCCAGCAGCA AAAGTCTGCA GGAGCTCATC AAAGAGGTGG AGGATCTTCA GGAGCAGCTG 
GGTACTTTCC CAGTAAGCAT CAGTTCAGAA ACAAATTTAA GCAAGGAAAT GGAGGCCGTT 
ATGAAGGATA TTAAAAGCAC TACGCAGAAG AAATACACAG ACTATAGCAA GACTCAAGGT 
TCCCCAGACA ACGACTTTCT CTTCATGTAT TCAGTGGCAA GAACTAATTT GGAACTGGAG 
CTTATACATT GTGGAGACAA TCTTTGCTCT GGAGGGGCCA GTGCTGCCGG GAAAAGATCA 
TGTCTGAATC AGCTTTTTCA TGTATTAGCT ATGCACATGC GATTATACAG CATTGATCCC 
GAATACAACC CCTGGAAAAA GTTAACCCAA GTCATACAGA AAAAACCTTC CCTCGGAGAC 
GATGAGGAGA GAGAAGAGGT TCCGATACTT TTCCGAGATG TCACCTCTCT TTTGCTCATT 
CAGGTTCTAA CAATGCCCCA GCCATTGTGC AAAGAGCATT TTGTTTGCAT TGTGAAAGTC 
CTCTACACGT TGCTATACAC TCAAGCGATC ACATCCCTCT CGGTAAAACT CCAGGCTGAA 
GATCGCAAGG TTTGGAGGGA ATCTGGATCC AGCAAGAAGA GTTCATATTC TTCTGGAAAA 
CCTTGGGAAG CTTTGCTTGG GTTTGTGATA AGTGAACTAT CAAGAGGGAG CTTATACCAA 
GATGAAGAGA CTGACAGTAT AAACATGAGT GACTTCACAG CTTGGTACGA AGACGCCATT 
GAGCATTATT TACAGCAGTT TTGCTTACCT TTCCTCAGGA TTACAAGCTT TCTCCAGCAT 
CACCTTTTCG GATTAGATTT GCCAAGTTGC CAGGAAGAAG ATGAATTTAC TGTCCTTTCC 
AACTGTTTGG GACTTTTGCC ATCTTTCAGT GAAGCATCGC ACCAGTTTGT CAGTGCCTCC 
TTACTTGACT GGCCAGTGTC CGCATTTGAT TTAATATCTC AGTGGTGTTC AGAAGTCAAT 
TTGTTTTCAG ACAAGCACCC AGACCAAGCT AACGTTTTGC TTATTCAAGA TACCAAGTGG 
GACTTACCAC GCCTCCTTCA GTTGCCAGAA AACTACAATA CAATATTTCA ATATTATCAT 
AGAAAGTGCT GCAGTGTCTG TACAAAGGTT CCCAAGGATC CTGCACTTTG CCTAGTTTGT 
GGCACATTTG TTTGCTTAAA GGGCCTCTGC TGCAAACAAC AGAGTTACTG TGAATGTGTA 
TTGCACTCCC AAAACTGTGG AGCCGGCACT GGAATTTTCC TCTTGATCAA CGCTTCTGTC 
ATCATTATTA TTCGAGGCCA TCGCTTCTGC CTTTGGGGCT CTGTATATCT CGATGCACAT 
GGGGAGGAAG ACAGAGACCT TAGGCGCGGA AAGCCCCTGT ACATCTGTAA GGAAAGGTAC 
AGCATGCTGG AGCAGCAATG GGTCTCGCAC ACGTTTGATC ACATCAATAA GAGATGGGGG 
CCGCATTACA ATGGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826536.1
CDS	197..5797
Translation	MMAAETPISRDKEATAALLKSKLSRPDQSPGLERLNGLLDRVLCVGGPAGEALEWCKCLLAGGDKFEDFCQAVRSYDNATLCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGEHAGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHRVKLSSCVPSVPQDLLVMSELVIPRFIFYLIQYLRDGYSEPGAEASPEKDLQKTLQMLDPQISFLEDLTKMGGAMRSVLTQVLTNQQLYKDLSSGPGESTCSKSHEKYLIALKESGLSIPEENVSSGAQEQSPGTSTLAVKEVAGAEGAQGQLESSDEEDQDGRQCVGKRKRVKLSSNTKDQSIMDMLKHKCFLEELLFWTIKYEFPQKMVTFLLNMLPDQDYKIAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKKFLEDHGLLVTWMNFVSFFQGMNLNKRELTEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVPDTQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAIKCQEIDLDSILPDQEMLMKLMVHPLLIQASLSEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASKLDPDYFISSVFERFKVVDLLTMASQHQNTVLDADHERSMLEGALTFLVILLSLRLHLGMTDDEILRAEMISQLCMNDRTHSSLLDLIPESPNPKSGIMPGSCSFESVLSAVADFKAPVFEPGGSMQQGMYTPKGDIWDQEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGRFSGNPWPPYKKRSPLHHSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNMSEHVLCMVLYLIELGLENSPEDESCEENFAGPDRCHDSWFPGSNLLCNMRHFINYVRVRVPETAPEVRREPPASTSTDGPSSAQNARRPSLLQRENSGTAQVFSLVAERRKKFQEIINRNNSEANQVVRPKSSALKWTAPGATPQLTTVILEVKESMLSLLIKLHKKLSGKQDSYCPPWLDDPECLVQPDLPGYCHGDGVTAVERILLKAASQSRLNRQSIDEICKKAPPPVPPKKSSPADKKTLGKEERRQKARERQQKLLAEFASRQKSFMETAMDVESPESDVAMEIAGAEQHVAEPIYDCVICGQSGPSTEDRPLGLVVLLQASSVLGHRRKSTEPKKLPTTEEEQIYQEGTCAAANDVKLAILQRYFKDSSCLQSVSVGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCFPGDSTERGSWHSHAASSKSLQELIKEVEDLQEQLGTFPVSISSETNLSKEMEAVMKDIKSTTQKKYTDYSKTQGSPDNDFLFMYSVARTNLELELIHCGDNLCSGGASAAGKRSCLNQLFHVLAMHMRLYSIDPEYNPWKKLTQVIQKKPSLGDDEEREEVPILFRDVTSLLLIQVLTMPQPLCKEHFVCIVKVLYTLLYTQAITSLSVKLQAEDRKVWRESGSSKKSSYSSGKPWEALLGFVISELSRGSLYQDEETDSINMSDFTAWYEDAIEHYLQQFCLPFLRITSFLQHHLFGLDLPSCQEEDEFTVLSNCLGLLPSFSEASHQFVSASLLDWPVSAFDLISQWCSEVNLFSDKHPDQANVLLIQDTKWDLPRLLQLPENYNTIFQYYHRKCCSVCTKVPKDPALCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYSMLEQQWVSHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_012971082.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971082.2

ORF Insert Sequence:

ATGATGGCGG CGGAGACGCC GATAAGCCGG GATAAAGAAG CCACAGCCGC TTTGCTGAAG 
AGCAAACTGA GCCGGCCGGA CCAGAGCCCG GGGTTGGAGC GGCTGAACGG ATTGCTGGAC 
CGGGTTCTGT GCGTAGGAGG GCCGGCCGGG GAGGCCCTGG AGTGGTGCAA GTGCCTGCTG 
GCCGGGGGGG ACAAGTTCGA GGACTTCTGT CAGGCTGTGC GCTCCTATGA CAATGCCACC 
CTGTGCGGCC TGGTCTGGAC GGCGAACTTC GTGGCCTATC GGTGCCGGAC GTGCGGGATA 
TCCCCATGCA TGTCCCTGTG CGCAGAGTGC TTCCACCAGG GCGAACATGC CGGCCATGAC 
TTTAACATGT TCCGCAGCCA GGCCGGGGGG GCTTGTGACT GTGGGGACAG CAATGTCATG 
AGGGAGAGCG GGTTTTGTAA AAGACATAGA GTGAAGCTGA GCTCCTGTGT TCCCTCTGTG 
CCCCAAGACT TGTTGGTGAT GTCTGAATTG GTTATACCTC GTTTTATCTT TTATCTCATT 
CAGTATCTGA GAGATGGGTA TAGTGAACCA GGAGCTGAAG CATCGCCGGA AAAAGATCTA 
CAGAAAACCC TTCAGATGCT GGATCCTCAG ATCTCCTTCT TGGAAGATTT AACCAAGATG 
GGTGGAGCTA TGAGGTCTGT TCTCACCCAG GTCCTGACCA ACCAGCAGCT TTATAAAGAC 
CTTTCTTCTG GTCCTGGAGA GAGTACATGC TCTAAGAGCC ATGAAAAGTA TCTCATAGCT 
CTGAAGGAAT CTGGTTTGTC AATTCCAGAA GAGAATGTTT CCAGTGGGGC ACAGGAGCAG 
TCACCTGGCA CAAGTACACT AGCAGTTAAA GAGGTTGCAG GTGCAGAAGG TGCCCAAGGG 
CAGCTAGAAT CTTCAGATGA GGAGGACCAG GATGGCAGGC AGTGTGTTGG GAAAAGAAAA 
AGAGTGAAGC TAAGCAGTAA CACCAAAGAT CAATCTATCA TGGATATGTT GAAACACAAA 
TGTTTTCTAG AAGAACTGTT ATTTTGGACA ATAAAGTATG AATTTCCACA GAAAATGGTC 
ACTTTCTTAT TGAATATGTT ACCAGATCAA GATTATAAGA TTGCATTTAC GAAAACATTT 
GTCCAGCATT ATGCTTTCAT TATGAAGACG TTGAAGAAAA GCCATGAATC GGACACTATG 
TCCAATCGAA TTGTGCACAT CAGTGTCCAG CTGTTCAGCA ATGAGGAGCT GGCGCGTCAA 
GTCACAGAAG AGTGCCAACT TCTGGACATT ATGGTCACTG TGCTACTGTA CATGATGGAA 
AGTTGCCTTA TTAAAAGTGA ACTGCAAGAT GAAGAAAACA GTTTACATGT GGTGGTGAAT 
TGTGGAGAAG CTTTACTGAA AAACAATACC TACTGGCCCC TTGTTAGTGA TTTTATTAAT 
ATCCTCTCTC ACCAAAGTGT AGCTAAGAAA TTCCTAGAGG ATCACGGGCT ATTAGTTACT 
TGGATGAACT TTGTATCATT CTTTCAAGGT ATGAATTTAA ACAAAAGAGA GCTCACCGAA 
CATGTAGAAT TTGAATCCCA GACATACTAT GCTGCATTTG CTGCTGAACT TGAAGCCTGT 
GCCCAGCCCA TGTGGGGACT TTTATCGCAC TGCAAAGTTC CAGATACACA AGAATACACA 
CGAAATGTTG TTCGATATTG CCTTGAAGCT CTTCAGGATT GGTTTGATGC TATCAATTTT 
GTAGATGAGC CTGCTCCAAA CCAGGTCACA TTTCACTTGC CTCTGCATCG ATACTATGCT 
ATGTTTTTAA GCAAGGCTAT AAAATGCCAA GAAATAGATT TAGATTCTAT TTTGCCTGAC 
CAGGAAATGC TGATGAAACT AATGGTTCAT CCACTACTAA TTCAGGCAAG TTTGTCTGAA 
ATACACAGCA ACATGTGGGT TCGGAATGGT CTCCAGATTA AAGGACAGGC TATGACATAT 
GTGCAGTCAC ATTTTTGCAA TTCCATGATC GACCCAGATA TCTATCTTCT ACAGGTCTGT 
GCTTCAAAAC TTGACCCAGA CTACTTTATT TCATCAGTCT TTGAAAGATT TAAAGTTGTA 
GATCTATTAA CGATGGCATC TCAGCACCAA AACACAGTGC TCGACGCAGA TCATGAACGG 
TCAATGCTTG AGGGAGCTTT AACATTTCTC GTCATTCTTT TAAGCCTCCG CTTGCATTTA 
GGAATGACAG ATGATGAGAT TCTCCGCGCA GAGATGATAT CCCAGCTCTG TATGAATGAC 
CGCACACACA GCTCACTCCT TGACCTTATT CCAGAAAGCC CAAACCCAAA AAGTGGTATT 
ATGCCAGGGA GTTGCAGCTT TGAATCTGTG TTGTCTGCAG TTGCTGATTT TAAAGCTCCC 
GTGTTTGAGC CTGGAGGCTC AATGCAACAA GGAATGTATA CTCCCAAGGG TGACATCTGG 
GATCAGGAAT TTGACCCCGT AATGGTGATT CTGAGGACAG TTTACAGGAG AGATGTACAG 
TCTGCCATGG ACAGATACAC AGCATTTTTA AAGCAGTCTG GGAGATTTTC TGGAAACCCA 
TGGCCGCCTT ACAAGAAAAG GAGCCCTCTG CATCACAGCT ATAAAGGCCT TATGAGGCTA 
TTGCACTGCA AAACATTGCA CATTGTGCTG TTTACACTGC TTTATAAGAT TCTGATGGAT 
CATCAGAATA TGTCAGAACA CGTTCTTTGT ATGGTTTTGT ATCTCATAGA GCTTGGGCTA 
GAAAATTCTC CCGAGGACGA GTCATGTGAA GAGAATTTTG CTGGGCCAGA TAGATGCCAC 
GACAGCTGGT TTCCTGGAAG TAACCTACTG TGCAACATGC GCCACTTTAT TAACTACGTC 
AGAGTGCGAG TACCAGAGAC TGCTCCAGAG GTGAGGAGGG AGCCCCCAGC AAGCACTAGC 
ACTGACGGAC CATCCTCGGC ACAGAATGCA AGACGGCCAT CTCTGCTCCA GAGGGAGAAT 
TCAGGTACAG CTCAGGTTTT CAGCTTGGTA GCAGAACGTC GGAAAAAGTT CCAGGAGATA 
ATTAACCGCA ATAACAGTGA AGCCAATCAA GTTGTGCGCC CCAAATCTTC AGCTCTGAAG 
TGGACGGCAC CCGGTGCAAC TCCTCAGTTA ACGACAGTCA TATTAGAGGT GAAAGAGAGC 
ATGCTATCCC TGCTTATTAA ACTGCACAAG AAGCTCTCGG GAAAGCAGGA TTCCTATTGC 
CCCCCCTGGT TGGATGATCC AGAATGCCTT GTCCAGCCGG ACTTACCTGG GTATTGTCAT 
GGCGATGGTG TAACCGCTGT AGAACGAATT TTACTGAAAG CAGCATCACA AAGTCGTCTG 
AACAGACAGA GTATTGATGA GATATGCAAG AAAGCTCCTC CACCCGTGCC ACCCAAAAAA 
TCTAGTCCTG CTGACAAGAA GACTTTGGGT AAAGAAGAAA GACGGCAGAA AGCGAGGGAG 
AGGCAACAGA AATTGCTTGC GGAATTCGCC TCAAGGCAAA AAAGCTTCAT GGAAACAGCG 
ATGGATGTTG AATCACCTGA AAGTGATGTG GCAATGGAAA TAGCTGGAGC TGAACAGCAC 
GTGGCAGAAC CAATATATGA TTGTGTTATA TGTGGACAGA GTGGCCCATC TACAGAAGAT 
CGCCCTCTAG GGCTTGTTGT TCTGCTTCAG GCATCTTCTG TTTTGGGGCA TCGGCGTAAG 
AGCACAGAGC CAAAGAAACT ACCAACCACA GAGGAGGAGC AGATATACCA AGAGGGCACA 
TGTGCAGCAG CTAATGATGT CAAACTTGCA ATATTACAGA GATATTTCAA AGATAGCTCC 
TGTCTTCAGT CAGTATCAGT TGGTTGGGAA GGCGGCGTCT ATGTTCAGAC CTGTGGCCAT 
ACTCTGCATA TTGACTGCCA TAAATCTTAT ATGGAATCCT TACGGAATGA CCAGGTTTTA 
CAAGGCTTTT CTGTAGACAA AGGGGAATTT ACATGCCCGC TGTGCAGGCA GTTTGCAAAC 
AGCGTCCTTC CATGCTTCCC GGGAGACAGT ACAGAGAGGG GGTCGTGGCA CAGCCATGCA 
GCCAGCAGCA AAAGTCTGCA GGAGCTCATC AAAGAGGTGG AGGATCTTCA GGAGCAGCTG 
GGTACTTTCC CAGTAAGCAT CAGTTCAGAA ACAAATTTAA GCAAGGAAAT GGAGGCCGTT 
ATGAAGGATA TTAAAAGCAC TACGCAGAAG AAATACACAG ACTATAGCAA GACTCAAGGT 
TCCCCAGACA ACGACTTTCT CTTCATGTAT TCAGTGGCAA GAACTAATTT GGAACTGGAG 
CTTATACATT GTGGAGACAA TCTTTGCTCT GGAGGGGCCA GTGCTGCCGG GAAAAGATCA 
TGTCTGAATC AGCTTTTTCA TGTATTAGCT ATGCACATGC GATTATACAG CATTGATCCC 
GAATACAACC CCTGGAAAAA GTTAACCCAA GTCATACAGA AAAAACCTTC CCTCGGAGAC 
GATGAGGAGA GAGAAGAGGT TCCGATACTT TTCCGAGATG TCACCTCTCT TTTGCTCATT 
CAGGTTCTAA CAATGCCCCA GCCATTGTGC AAAGAGCATT TTGTTTGCAT TGTGAAAGTC 
CTCTACACGT TGCTATACAC TCAAGCGATC ACATCCCTCT CGGTAAAACT CCAGGCTGAA 
GATCGCAAGG TTTGGAGGGA ATCTGGATCC AGCAAGAAGA GTTCATATTC TTCTGGAAAA 
CCTTGGGAAG CTTTGCTTGG GTTTGTGATA AGTGAACTAT CAAGAGGGAG CTTATACCAA 
GATGAAGAGA CTGACAGTAT AAACATGAGT GACTTCACAG CTTGGTACGA AGACGCCATT 
GAGCATTATT TACAGCAGTT TTGCTTACCT TTCCTCAGGA TTACAAGCTT TCTCCAGCAT 
CACCTTTTCG GATTAGATTT GCCAAGTTGC CAGGAAGAAG ATGAATTTAC TGTCCTTTCC 
AACTGTTTGG GACTTTTGCC ATCTTTCAGT GAAGCATCGC ACCAGTTTGT CAGTGCCTCC 
TTACTTGACT GGCCAGTGTC CGCATTTGAT TTAATATCTC AGTGGTGTTC AGAAGTCAAT 
TTGTTTTCAG ACAAGCACCC AGACCAAGCT AACGTTTTGC TTATTCAAGA TACCAAGTGG 
GACTTACCAC GCCTCCTTCA GTTGCCAGAA AACTACAATA CAATATTTCA ATATTATCAT 
AGAAAGTGCT GCAGTGTCTG TACAAAGGTT CCCAAGGATC CTGCACTTTG CCTAGTTTGT 
GGCACATTTG TTTGCTTAAA GGGCCTCTGC TGCAAACAAC AGAGTTACTG TGAATGTGTA 
TTGCACTCCC AAAACTGTGG AGCCGGCACT GGAATTTTCC TCTTGATCAA CGCTTCTGTC 
ATCATTATTA TTCGAGGCCA TCGCTTCTGC CTTTGGGGCT CTGTATATCT CGATGCACAT 
GGGGAGGAAG ACAGAGACCT TAGGCGCGGA AAGCCCCTGT ACATCTGTAA GGAAAGGTAC 
AGCATGCTGG AGCAGCAATG GGTCTCGCAC ACGTTTGATC ACATCAATAA GAGATGGGGG 
CCGCATTACA ATGGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr3 ( XM_012971083.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826537.1 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X2, mRNA.

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CloneID	OXa37227
Clone ID Related Accession (Same CDS sequence)	XM_012971083.2 , XM_012971083.3
Accession Version	XM_012971083.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971083.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CGGAGACGCC GATAAGCCGG GATAAAGAAG CCACAGCCGC TTTGCTGAAG 
AGCAAACTGA GCCGGCCGGA CCAGAGCCCG GGGTTGGAGC GGCTGAACGG ATTGCTGGAC 
CGGGTTCTGT GCGTAGGAGG GCCGGCCGGG GAGGCCCTGG AGTGGTGCAA GTGCCTGCTG 
GCCGGGGGGG ACAAGTTCGA GGACTTCTGT CAGGCTGTGC GCTCCTATGA CAATGCCACC 
CTGTGCGGCC TGGTCTGGAC GGCGAACTTC GTGGCCTATC GGTGCCGGAC GTGCGGGATA 
TCCCCATGCA TGTCCCTGTG CGCAGAGTGC TTCCACCAGG GCGAACATGC CGGCCATGAC 
TTTAACATGT TCCGCAGCCA GGCCGGGGGG GCTTGTGACT GTGGGGACAG CAATGTCATG 
AGGGAGAGCG GGTTTTGTAA AAGACATAGA GTGAAGCTGA GCTCCTGTGT TCCCTCTGTG 
CCCCAAGACT TGTTGGTGAT GTCTGAATTG GTTATACCTC GTTTTATCTT TTATCTCATT 
CAGTATCTGA GAGATGGGTA TAGTGAACCA GGAGCTGAAG CATCGCCGGA AAAAGATCTA 
CAGAAAACCC TTCAGATGCT GGATCCTCAG ATCTCCTTCT TGGAAGATTT AACCAAGATG 
GGTGGAGCTA TGAGGTCTGT TCTCACCCAG GTCCTGACCA ACCAGCAGCT TTATAAAGAC 
CTTTCTTCTG GTCCTGGAGA GAGTACATGC TCTAAGAGCC ATGAAAAGTA TCTCATAGCT 
CTGAAGGAAT CTGGTTTGTC AATTCCAGAA GAGAATGTTT CCAGTGGGGC ACAGGAGCAG 
TCACCTGGCA CAAGTACACT AGCAGTTAAA GAGGTTGCAG GTGCAGAAGG TGCCCAAGGG 
CAGCTAGAAT CTTCAGATGA GGAGGACCAG GATGGCAGGC AGTGTGTTGG GAAAAGAAAA 
AGAGTGAAGC TAAGCAGTAA CACCAAAGAT CAATCTATCA TGGATATGTT GAAACACAAA 
TGTTTTCTAG AAGAACTGTT ATTTTGGACA ATAAAGTATG AATTTCCACA GAAAATGGTC 
ACTTTCTTAT TGAATATGTT ACCAGATCAA GATTATAAGA TTGCATTTAC GAAAACATTT 
GTCCAGCATT ATGCTTTCAT TATGAAGACG TTGAAGAAAA GCCATGAATC GGACACTATG 
TCCAATCGAA TTGTGCACAT CAGTGTCCAG CTGTTCAGCA ATGAGGAGCT GGCGCGTCAA 
GTCACAGAAG AGTGCCAACT TCTGGACATT ATGGTCACTG TGCTACTGTA CATGATGGAA 
AGTTGCCTTA TTAAAAGTGA ACTGCAAGAT GAAGAAAACA GTTTACATGT GGTGGTGAAT 
TGTGGAGAAG CTTTACTGAA AAACAATACC TACTGGCCCC TTGTTAGTGA TTTTATTAAT 
ATCCTCTCTC ACCAAAGTGT AGCTAAGAAA TTCCTAGAGG ATCACGGGCT ATTAGTTACT 
TGGATGAACT TTGTATCATT CTTTCAAGGT ATGAATTTAA ACAAAAGAGA GCTCACCGAA 
CATGTAGAAT TTGAATCCCA GACATACTAT GCTGCATTTG CTGCTGAACT TGAAGCCTGT 
GCCCAGCCCA TGTGGGGACT TTTATCGCAC TGCAAAGTTC CAGATACACA AGAATACACA 
CGAAATGTTG TTCGATATTG CCTTGAAGCT CTTCAGGATT GGTTTGATGC TATCAATTTT 
GTAGATGAGC CTGCTCCAAA CCAGGTCACA TTTCACTTGC CTCTGCATCG ATACTATGCT 
ATGTTTTTAA GCAAGGCTAT AAAATGCCAA GAAATAGATT TAGATTCTAT TTTGCCTGAC 
CAGGAAATGC TGATGAAACT AATGGTTCAT CCACTACTAA TTCAGGCAAG TTTGTCTGAA 
ATACACAGCA ACATGTGGGT TCGGAATGGT CTCCAGATTA AAGGACAGGC TATGACATAT 
GTGCAGTCAC ATTTTTGCAA TTCCATGATC GACCCAGATA TCTATCTTCT ACAGGTCTGT 
GCTTCAAAAC TTGACCCAGA CTACTTTATT TCATCAGTCT TTGAAAGATT TAAAGTTGTA 
GATCTATTAA CGATGGCATC TCAGCACCAA AACACAGTGC TCGACGCAGA TCATGAACGG 
TCAATGCTTG AGGGAGCTTT AACATTTCTC GTCATTCTTT TAAGCCTCCG CTTGCATTTA 
GGAATGACAG ATGATGAGAT TCTCCGCGCA GAGATGATAT CCCAGCTCTG TATGAATGAC 
CGCACACACA GCTCACTCCT TGACCTTATT CCAGAAAGCC CAAACCCAAA AAGTGGTATT 
ATGCCAGGGA GTTGCAGCTT TGAATCTGTG TTGTCTGCAG TTGCTGATTT TAAAGCTCCC 
GTGTTTGAGC CTGGAGGCTC AATGCAACAA GGAATGTATA CTCCCAAGGG TGACATCTGG 
GATCAGGAAT TTGACCCCGT AATGGTGATT CTGAGGACAG TTTACAGGAG AGATGTACAG 
TCTGCCATGG ACAGATACAC AGCATTTTTA AAGCAGTCTG GGAGATTTTC TGGAAACCCA 
TGGCCGCCTT ACAAGAAAAG GAGCCCTCTG CATCACAGCT ATAAAGGCCT TATGAGGCTA 
TTGCACTGCA AAACATTGCA CATTGTGCTG TTTACACTGC TTTATAAGAT TCTGATGGAT 
CATCAGAATA TGTCAGAACA CGTTCTTTGT ATGGTTTTGT ATCTCATAGA GCTTGGGCTA 
GAAAATTCTC CCGAGGACGA GTCATGTGAA GAGAATTTTG CTGGGCCAGA TAGATGCCAC 
GACAGCTGGT TTCCTGGAAG TAACCTACTG TGCAACATGC GCCACTTTAT TAACTACGTC 
AGAGTGCGAG TACCAGAGAC TGCTCCAGAG GTGAGGAGGG AGCCCCCAGC AAGCACTAGC 
ACTGACGGAC CATCCTCGGC ACAGAATGCA AGACGGCCAT CTCTGCTCCA GAGGGAGAAT 
TCAGGTACAG CTCAGGTTTT CAGCTTGGTA GCAGAACGTC GGAAAAAGTT CCAGGAGATA 
ATTAACCGCA ATAACAGTGA AGCCAATCAA GTTGTGCGCC CCAAATCTTC AGCTCTGAAG 
TGGACGGCAC CCGGTGCAAC TCCTCAGTTA ACGACAGTCA TATTAGAGGT GAAAGAGAGC 
ATGCTATCCC TGCTTATTAA ACTGCACAAG AAGCTCTCGG GAAAGCAGGA TTCCTATTGC 
CCCCCCTGGT TGGATGATCC AGAATGCCTT GTCCAGCCGG ACTTACCTGG GTATTGTCAT 
GGCGATGGTG TAACCGCTGT AGAACGAATT TTACTGAAAG CAGCATCACA AAGTCGTCTG 
AACAGACAGA GTATTGATGA GATATGCAAG AAAGCTCCTC CACCCGTGCC ACCCAAAAAA 
TCTAGTCCTG CTGACAAGAA GACTTTGGGT AAAGAAGAAA GACGGCAGAA AGCGAGGGAG 
AGGCAACAGA AATTGCTTGC GGAATTCGCC TCAAGGCAAA AAAGCTTCAT GGAAACAGCG 
ATGGATGTTG AATCACCTGA AAGTGATGTG GCAATGGAAA TAGCTGGAGC TGAACAGCAC 
GTGGCAGAAC CAATATATGA TTGTGTTATA TGTGGACAGA GTGGCCCATC TACAGAAGAT 
CGCCCTCTAG GGCTTGTTGT TCTGCTTCAG GCATCTTCTG TTTTGGGGCA TCGGCGTAAG 
AGCACAGAGC CAAAGAAACT ACCAACCACA GAGGAGGAGC AGATATACCA AGAGGGCACA 
TGTGCAGCAG CTAATGATGT CAAACTTGCA ATATTACAGA GATATTTCAA AGATAGCTCC 
TGTCTTCAGT CAGTATCAGT TGGTTGGGAA GGCGGCGTCT ATGTTCAGAC CTGTGGCCAT 
ACTCTGCATA TTGACTGCCA TAAATCTTAT ATGGAATCCT TACGGAATGA CCAGGTTTTA 
CAAGGCTTTT CTGTAGACAA AGGGGAATTT ACATGCCCGC TGTGCAGGCA GTTTGCAAAC 
AGCGTCCTTC CATGCTTCCC GGGAGACAGT ACAGAGAGGG GGTCGTGGCA CAGCCATGCA 
GCCAGCAGCA AAAGTCTGCA GGAGCTCATC AAAGAGGTGG AGGATCTTCA GGAGCAGCTG 
GGTACTTTCC CATCAGAAAC AAATTTAAGC AAGGAAATGG AGGCCGTTAT GAAGGATATT 
AAAAGCACTA CGCAGAAGAA ATACACAGAC TATAGCAAGA CTCAAGGTTC CCCAGACAAC 
GACTTTCTCT TCATGTATTC AGTGGCAAGA ACTAATTTGG AACTGGAGCT TATACATTGT 
GGAGACAATC TTTGCTCTGG AGGGGCCAGT GCTGCCGGGA AAAGATCATG TCTGAATCAG 
CTTTTTCATG TATTAGCTAT GCACATGCGA TTATACAGCA TTGATCCCGA ATACAACCCC 
TGGAAAAAGT TAACCCAAGT CATACAGAAA AAACCTTCCC TCGGAGACGA TGAGGAGAGA 
GAAGAGGTTC CGATACTTTT CCGAGATGTC ACCTCTCTTT TGCTCATTCA GGTTCTAACA 
ATGCCCCAGC CATTGTGCAA AGAGCATTTT GTTTGCATTG TGAAAGTCCT CTACACGTTG 
CTATACACTC AAGCGATCAC ATCCCTCTCG GTAAAACTCC AGGCTGAAGA TCGCAAGGTT 
TGGAGGGAAT CTGGATCCAG CAAGAAGAGT TCATATTCTT CTGGAAAACC TTGGGAAGCT 
TTGCTTGGGT TTGTGATAAG TGAACTATCA AGAGGGAGCT TATACCAAGA TGAAGAGACT 
GACAGTATAA ACATGAGTGA CTTCACAGCT TGGTACGAAG ACGCCATTGA GCATTATTTA 
CAGCAGTTTT GCTTACCTTT CCTCAGGATT ACAAGCTTTC TCCAGCATCA CCTTTTCGGA 
TTAGATTTGC CAAGTTGCCA GGAAGAAGAT GAATTTACTG TCCTTTCCAA CTGTTTGGGA 
CTTTTGCCAT CTTTCAGTGA AGCATCGCAC CAGTTTGTCA GTGCCTCCTT ACTTGACTGG 
CCAGTGTCCG CATTTGATTT AATATCTCAG TGGTGTTCAG AAGTCAATTT GTTTTCAGAC 
AAGCACCCAG ACCAAGCTAA CGTTTTGCTT ATTCAAGATA CCAAGTGGGA CTTACCACGC 
CTCCTTCAGT TGCCAGAAAA CTACAATACA ATATTTCAAT ATTATCATAG AAAGTGCTGC 
AGTGTCTGTA CAAAGGTTCC CAAGGATCCT GCACTTTGCC TAGTTTGTGG CACATTTGTT 
TGCTTAAAGG GCCTCTGCTG CAAACAACAG AGTTACTGTG AATGTGTATT GCACTCCCAA 
AACTGTGGAG CCGGCACTGG AATTTTCCTC TTGATCAACG CTTCTGTCAT CATTATTATT 
CGAGGCCATC GCTTCTGCCT TTGGGGCTCT GTATATCTCG ATGCACATGG GGAGGAAGAC 
AGAGACCTTA GGCGCGGAAA GCCCCTGTAC ATCTGTAAGG AAAGGTACAG CATGCTGGAG 
CAGCAATGGG TCTCGCACAC GTTTGATCAC ATCAATAAGA GATGGGGGCC GCATTACAAT 
GGCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826537.1
CDS	197..5785
Translation	MMAAETPISRDKEATAALLKSKLSRPDQSPGLERLNGLLDRVLCVGGPAGEALEWCKCLLAGGDKFEDFCQAVRSYDNATLCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGEHAGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHRVKLSSCVPSVPQDLLVMSELVIPRFIFYLIQYLRDGYSEPGAEASPEKDLQKTLQMLDPQISFLEDLTKMGGAMRSVLTQVLTNQQLYKDLSSGPGESTCSKSHEKYLIALKESGLSIPEENVSSGAQEQSPGTSTLAVKEVAGAEGAQGQLESSDEEDQDGRQCVGKRKRVKLSSNTKDQSIMDMLKHKCFLEELLFWTIKYEFPQKMVTFLLNMLPDQDYKIAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKKFLEDHGLLVTWMNFVSFFQGMNLNKRELTEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVPDTQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAIKCQEIDLDSILPDQEMLMKLMVHPLLIQASLSEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASKLDPDYFISSVFERFKVVDLLTMASQHQNTVLDADHERSMLEGALTFLVILLSLRLHLGMTDDEILRAEMISQLCMNDRTHSSLLDLIPESPNPKSGIMPGSCSFESVLSAVADFKAPVFEPGGSMQQGMYTPKGDIWDQEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGRFSGNPWPPYKKRSPLHHSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNMSEHVLCMVLYLIELGLENSPEDESCEENFAGPDRCHDSWFPGSNLLCNMRHFINYVRVRVPETAPEVRREPPASTSTDGPSSAQNARRPSLLQRENSGTAQVFSLVAERRKKFQEIINRNNSEANQVVRPKSSALKWTAPGATPQLTTVILEVKESMLSLLIKLHKKLSGKQDSYCPPWLDDPECLVQPDLPGYCHGDGVTAVERILLKAASQSRLNRQSIDEICKKAPPPVPPKKSSPADKKTLGKEERRQKARERQQKLLAEFASRQKSFMETAMDVESPESDVAMEIAGAEQHVAEPIYDCVICGQSGPSTEDRPLGLVVLLQASSVLGHRRKSTEPKKLPTTEEEQIYQEGTCAAANDVKLAILQRYFKDSSCLQSVSVGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCFPGDSTERGSWHSHAASSKSLQELIKEVEDLQEQLGTFPSETNLSKEMEAVMKDIKSTTQKKYTDYSKTQGSPDNDFLFMYSVARTNLELELIHCGDNLCSGGASAAGKRSCLNQLFHVLAMHMRLYSIDPEYNPWKKLTQVIQKKPSLGDDEEREEVPILFRDVTSLLLIQVLTMPQPLCKEHFVCIVKVLYTLLYTQAITSLSVKLQAEDRKVWRESGSSKKSSYSSGKPWEALLGFVISELSRGSLYQDEETDSINMSDFTAWYEDAIEHYLQQFCLPFLRITSFLQHHLFGLDLPSCQEEDEFTVLSNCLGLLPSFSEASHQFVSASLLDWPVSAFDLISQWCSEVNLFSDKHPDQANVLLIQDTKWDLPRLLQLPENYNTIFQYYHRKCCSVCTKVPKDPALCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYSMLEQQWVSHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_012971083.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971083.2

ORF Insert Sequence:

ATGATGGCGG CGGAGACGCC GATAAGCCGG GATAAAGAAG CCACAGCCGC TTTGCTGAAG 
AGCAAACTGA GCCGGCCGGA CCAGAGCCCG GGGTTGGAGC GGCTGAACGG ATTGCTGGAC 
CGGGTTCTGT GCGTAGGAGG GCCGGCCGGG GAGGCCCTGG AGTGGTGCAA GTGCCTGCTG 
GCCGGGGGGG ACAAGTTCGA GGACTTCTGT CAGGCTGTGC GCTCCTATGA CAATGCCACC 
CTGTGCGGCC TGGTCTGGAC GGCGAACTTC GTGGCCTATC GGTGCCGGAC GTGCGGGATA 
TCCCCATGCA TGTCCCTGTG CGCAGAGTGC TTCCACCAGG GCGAACATGC CGGCCATGAC 
TTTAACATGT TCCGCAGCCA GGCCGGGGGG GCTTGTGACT GTGGGGACAG CAATGTCATG 
AGGGAGAGCG GGTTTTGTAA AAGACATAGA GTGAAGCTGA GCTCCTGTGT TCCCTCTGTG 
CCCCAAGACT TGTTGGTGAT GTCTGAATTG GTTATACCTC GTTTTATCTT TTATCTCATT 
CAGTATCTGA GAGATGGGTA TAGTGAACCA GGAGCTGAAG CATCGCCGGA AAAAGATCTA 
CAGAAAACCC TTCAGATGCT GGATCCTCAG ATCTCCTTCT TGGAAGATTT AACCAAGATG 
GGTGGAGCTA TGAGGTCTGT TCTCACCCAG GTCCTGACCA ACCAGCAGCT TTATAAAGAC 
CTTTCTTCTG GTCCTGGAGA GAGTACATGC TCTAAGAGCC ATGAAAAGTA TCTCATAGCT 
CTGAAGGAAT CTGGTTTGTC AATTCCAGAA GAGAATGTTT CCAGTGGGGC ACAGGAGCAG 
TCACCTGGCA CAAGTACACT AGCAGTTAAA GAGGTTGCAG GTGCAGAAGG TGCCCAAGGG 
CAGCTAGAAT CTTCAGATGA GGAGGACCAG GATGGCAGGC AGTGTGTTGG GAAAAGAAAA 
AGAGTGAAGC TAAGCAGTAA CACCAAAGAT CAATCTATCA TGGATATGTT GAAACACAAA 
TGTTTTCTAG AAGAACTGTT ATTTTGGACA ATAAAGTATG AATTTCCACA GAAAATGGTC 
ACTTTCTTAT TGAATATGTT ACCAGATCAA GATTATAAGA TTGCATTTAC GAAAACATTT 
GTCCAGCATT ATGCTTTCAT TATGAAGACG TTGAAGAAAA GCCATGAATC GGACACTATG 
TCCAATCGAA TTGTGCACAT CAGTGTCCAG CTGTTCAGCA ATGAGGAGCT GGCGCGTCAA 
GTCACAGAAG AGTGCCAACT TCTGGACATT ATGGTCACTG TGCTACTGTA CATGATGGAA 
AGTTGCCTTA TTAAAAGTGA ACTGCAAGAT GAAGAAAACA GTTTACATGT GGTGGTGAAT 
TGTGGAGAAG CTTTACTGAA AAACAATACC TACTGGCCCC TTGTTAGTGA TTTTATTAAT 
ATCCTCTCTC ACCAAAGTGT AGCTAAGAAA TTCCTAGAGG ATCACGGGCT ATTAGTTACT 
TGGATGAACT TTGTATCATT CTTTCAAGGT ATGAATTTAA ACAAAAGAGA GCTCACCGAA 
CATGTAGAAT TTGAATCCCA GACATACTAT GCTGCATTTG CTGCTGAACT TGAAGCCTGT 
GCCCAGCCCA TGTGGGGACT TTTATCGCAC TGCAAAGTTC CAGATACACA AGAATACACA 
CGAAATGTTG TTCGATATTG CCTTGAAGCT CTTCAGGATT GGTTTGATGC TATCAATTTT 
GTAGATGAGC CTGCTCCAAA CCAGGTCACA TTTCACTTGC CTCTGCATCG ATACTATGCT 
ATGTTTTTAA GCAAGGCTAT AAAATGCCAA GAAATAGATT TAGATTCTAT TTTGCCTGAC 
CAGGAAATGC TGATGAAACT AATGGTTCAT CCACTACTAA TTCAGGCAAG TTTGTCTGAA 
ATACACAGCA ACATGTGGGT TCGGAATGGT CTCCAGATTA AAGGACAGGC TATGACATAT 
GTGCAGTCAC ATTTTTGCAA TTCCATGATC GACCCAGATA TCTATCTTCT ACAGGTCTGT 
GCTTCAAAAC TTGACCCAGA CTACTTTATT TCATCAGTCT TTGAAAGATT TAAAGTTGTA 
GATCTATTAA CGATGGCATC TCAGCACCAA AACACAGTGC TCGACGCAGA TCATGAACGG 
TCAATGCTTG AGGGAGCTTT AACATTTCTC GTCATTCTTT TAAGCCTCCG CTTGCATTTA 
GGAATGACAG ATGATGAGAT TCTCCGCGCA GAGATGATAT CCCAGCTCTG TATGAATGAC 
CGCACACACA GCTCACTCCT TGACCTTATT CCAGAAAGCC CAAACCCAAA AAGTGGTATT 
ATGCCAGGGA GTTGCAGCTT TGAATCTGTG TTGTCTGCAG TTGCTGATTT TAAAGCTCCC 
GTGTTTGAGC CTGGAGGCTC AATGCAACAA GGAATGTATA CTCCCAAGGG TGACATCTGG 
GATCAGGAAT TTGACCCCGT AATGGTGATT CTGAGGACAG TTTACAGGAG AGATGTACAG 
TCTGCCATGG ACAGATACAC AGCATTTTTA AAGCAGTCTG GGAGATTTTC TGGAAACCCA 
TGGCCGCCTT ACAAGAAAAG GAGCCCTCTG CATCACAGCT ATAAAGGCCT TATGAGGCTA 
TTGCACTGCA AAACATTGCA CATTGTGCTG TTTACACTGC TTTATAAGAT TCTGATGGAT 
CATCAGAATA TGTCAGAACA CGTTCTTTGT ATGGTTTTGT ATCTCATAGA GCTTGGGCTA 
GAAAATTCTC CCGAGGACGA GTCATGTGAA GAGAATTTTG CTGGGCCAGA TAGATGCCAC 
GACAGCTGGT TTCCTGGAAG TAACCTACTG TGCAACATGC GCCACTTTAT TAACTACGTC 
AGAGTGCGAG TACCAGAGAC TGCTCCAGAG GTGAGGAGGG AGCCCCCAGC AAGCACTAGC 
ACTGACGGAC CATCCTCGGC ACAGAATGCA AGACGGCCAT CTCTGCTCCA GAGGGAGAAT 
TCAGGTACAG CTCAGGTTTT CAGCTTGGTA GCAGAACGTC GGAAAAAGTT CCAGGAGATA 
ATTAACCGCA ATAACAGTGA AGCCAATCAA GTTGTGCGCC CCAAATCTTC AGCTCTGAAG 
TGGACGGCAC CCGGTGCAAC TCCTCAGTTA ACGACAGTCA TATTAGAGGT GAAAGAGAGC 
ATGCTATCCC TGCTTATTAA ACTGCACAAG AAGCTCTCGG GAAAGCAGGA TTCCTATTGC 
CCCCCCTGGT TGGATGATCC AGAATGCCTT GTCCAGCCGG ACTTACCTGG GTATTGTCAT 
GGCGATGGTG TAACCGCTGT AGAACGAATT TTACTGAAAG CAGCATCACA AAGTCGTCTG 
AACAGACAGA GTATTGATGA GATATGCAAG AAAGCTCCTC CACCCGTGCC ACCCAAAAAA 
TCTAGTCCTG CTGACAAGAA GACTTTGGGT AAAGAAGAAA GACGGCAGAA AGCGAGGGAG 
AGGCAACAGA AATTGCTTGC GGAATTCGCC TCAAGGCAAA AAAGCTTCAT GGAAACAGCG 
ATGGATGTTG AATCACCTGA AAGTGATGTG GCAATGGAAA TAGCTGGAGC TGAACAGCAC 
GTGGCAGAAC CAATATATGA TTGTGTTATA TGTGGACAGA GTGGCCCATC TACAGAAGAT 
CGCCCTCTAG GGCTTGTTGT TCTGCTTCAG GCATCTTCTG TTTTGGGGCA TCGGCGTAAG 
AGCACAGAGC CAAAGAAACT ACCAACCACA GAGGAGGAGC AGATATACCA AGAGGGCACA 
TGTGCAGCAG CTAATGATGT CAAACTTGCA ATATTACAGA GATATTTCAA AGATAGCTCC 
TGTCTTCAGT CAGTATCAGT TGGTTGGGAA GGCGGCGTCT ATGTTCAGAC CTGTGGCCAT 
ACTCTGCATA TTGACTGCCA TAAATCTTAT ATGGAATCCT TACGGAATGA CCAGGTTTTA 
CAAGGCTTTT CTGTAGACAA AGGGGAATTT ACATGCCCGC TGTGCAGGCA GTTTGCAAAC 
AGCGTCCTTC CATGCTTCCC GGGAGACAGT ACAGAGAGGG GGTCGTGGCA CAGCCATGCA 
GCCAGCAGCA AAAGTCTGCA GGAGCTCATC AAAGAGGTGG AGGATCTTCA GGAGCAGCTG 
GGTACTTTCC CATCAGAAAC AAATTTAAGC AAGGAAATGG AGGCCGTTAT GAAGGATATT 
AAAAGCACTA CGCAGAAGAA ATACACAGAC TATAGCAAGA CTCAAGGTTC CCCAGACAAC 
GACTTTCTCT TCATGTATTC AGTGGCAAGA ACTAATTTGG AACTGGAGCT TATACATTGT 
GGAGACAATC TTTGCTCTGG AGGGGCCAGT GCTGCCGGGA AAAGATCATG TCTGAATCAG 
CTTTTTCATG TATTAGCTAT GCACATGCGA TTATACAGCA TTGATCCCGA ATACAACCCC 
TGGAAAAAGT TAACCCAAGT CATACAGAAA AAACCTTCCC TCGGAGACGA TGAGGAGAGA 
GAAGAGGTTC CGATACTTTT CCGAGATGTC ACCTCTCTTT TGCTCATTCA GGTTCTAACA 
ATGCCCCAGC CATTGTGCAA AGAGCATTTT GTTTGCATTG TGAAAGTCCT CTACACGTTG 
CTATACACTC AAGCGATCAC ATCCCTCTCG GTAAAACTCC AGGCTGAAGA TCGCAAGGTT 
TGGAGGGAAT CTGGATCCAG CAAGAAGAGT TCATATTCTT CTGGAAAACC TTGGGAAGCT 
TTGCTTGGGT TTGTGATAAG TGAACTATCA AGAGGGAGCT TATACCAAGA TGAAGAGACT 
GACAGTATAA ACATGAGTGA CTTCACAGCT TGGTACGAAG ACGCCATTGA GCATTATTTA 
CAGCAGTTTT GCTTACCTTT CCTCAGGATT ACAAGCTTTC TCCAGCATCA CCTTTTCGGA 
TTAGATTTGC CAAGTTGCCA GGAAGAAGAT GAATTTACTG TCCTTTCCAA CTGTTTGGGA 
CTTTTGCCAT CTTTCAGTGA AGCATCGCAC CAGTTTGTCA GTGCCTCCTT ACTTGACTGG 
CCAGTGTCCG CATTTGATTT AATATCTCAG TGGTGTTCAG AAGTCAATTT GTTTTCAGAC 
AAGCACCCAG ACCAAGCTAA CGTTTTGCTT ATTCAAGATA CCAAGTGGGA CTTACCACGC 
CTCCTTCAGT TGCCAGAAAA CTACAATACA ATATTTCAAT ATTATCATAG AAAGTGCTGC 
AGTGTCTGTA CAAAGGTTCC CAAGGATCCT GCACTTTGCC TAGTTTGTGG CACATTTGTT 
TGCTTAAAGG GCCTCTGCTG CAAACAACAG AGTTACTGTG AATGTGTATT GCACTCCCAA 
AACTGTGGAG CCGGCACTGG AATTTTCCTC TTGATCAACG CTTCTGTCAT CATTATTATT 
CGAGGCCATC GCTTCTGCCT TTGGGGCTCT GTATATCTCG ATGCACATGG GGAGGAAGAC 
AGAGACCTTA GGCGCGGAAA GCCCCTGTAC ATCTGTAAGG AAAGGTACAG CATGCTGGAG 
CAGCAATGGG TCTCGCACAC GTTTGATCAC ATCAATAAGA GATGGGGGCC GCATTACAAT 
GGCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr3 ( XM_012971084.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826538.1 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X3, mRNA.

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CloneID	OXa37228
Clone ID Related Accession (Same CDS sequence)	XM_012971084.2 , XM_012971084.3
Accession Version	XM_012971084.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971084.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CGGAGACGCC GATAAGCCGG GATAAAGAAG CCACAGCCGC TTTGCTGAAG 
AGCAAACTGA GCCGGCCGGA CCAGAGCCCG GGGTTGGAGC GGCTGAACGG ATTGCTGGAC 
CGGGTTCTGT GCGTAGGAGG GCCGGCCGGG GAGGCCCTGG AGTGGTGCAA GTGCCTGCTG 
GCCGGGGGGG ACAAGTTCGA GGACTTCTGT CAGGCTGTGC GCTCCTATGA CAATGCCACC 
CTGTGCGGCC TGGTCTGGAC GGCGAACTTC GTGGCCTATC GGTGCCGGAC GTGCGGGATA 
TCCCCATGCA TGTCCCTGTG CGCAGAGTGC TTCCACCAGG GCGAACATGC CGGCCATGAC 
TTTAACATGT TCCGCAGCCA GGCCGGGGGG GCTTGTGACT GTGGGGACAG CAATGTCATG 
AGGGAGAGCG GGTTTTGTAA AAGACATAGA GTGAAGCTGA GCTCCTGTGT TCCCTCTGTG 
CCCCAAGACT TGTTGGTGAT GTCTGAATTG GTTATACCTC GTTTTATCTT TTATCTCATT 
CAGTATCTGA GAGATGGGTA TAGTGAACCA GGAGCTGAAG CATCGCCGGA AAAAGATCTA 
CAGAAAACCC TTCAGATGCT GGATCCTCAG ATCTCCTTCT TGGAAGATTT AACCAAGATG 
GGTGGAGCTA TGAGGTCTGT TCTCACCCAG GTCCTGACCA ACCAGCAGCT TTATAAAGAC 
CTTTCTTCTG GTCCTGGAGA GAGTACATGC TCTAAGAGCC ATGAAAAGTA TCTCATAGCT 
CTGAAGGAAT CTGGTTTGTC AATTCCAGAA GAGAATGTTT CCAGTGGGGC ACAGGAGCAG 
TCACCTGGCA CAAGTACACT AGCAGTTAAA GAGGTTGCAG GTGCAGAAGG TGCCCAAGGG 
CAGCTAGAAT CTTCAGATGA GGAGGACCAG GATGGCAGGC AGTGTGTTGG GAAAAGAAAA 
AGAGTGAAGC TAAGCAGTAA CACCAAAGAT CAATCTATCA TGGATATGTT GAAACACAAA 
TGTTTTCTAG AAGAACTGTT ATTTTGGACA ATAAAGTATG AATTTCCACA GAAAATGGTC 
ACTTTCTTAT TGAATATGTT ACCAGATCAA GATTATAAGA TTGCATTTAC GAAAACATTT 
GTCCAGCATT ATGCTTTCAT TATGAAGACG TTGAAGAAAA GCCATGAATC GGACACTATG 
TCCAATCGAA TTGTGCACAT CAGTGTCCAG CTGTTCAGCA ATGAGGAGCT GGCGCGTCAA 
GTCACAGAAG AGTGCCAACT TCTGGACATT ATGGTCACTG TGCTACTGTA CATGATGGAA 
AGTTGCCTTA TTAAAAGTGA ACTGCAAGAT GAAGAAAACA GTTTACATGT GGTGGTGAAT 
TGTGGAGAAG CTTTACTGAA AAACAATACC TACTGGCCCC TTGTTAGTGA TTTTATTAAT 
ATCCTCTCTC ACCAAAGTGT AGCTAAGAAA TTCCTAGAGG ATCACGGGCT ATTAGTTACT 
TGGATGAACT TTGTATCATT CTTTCAAGGT ATGAATTTAA ACAAAAGAGA GCTCACCGAA 
CATGTAGAAT TTGAATCCCA GACATACTAT GCTGCATTTG CTGCTGAACT TGAAGCCTGT 
GCCCAGCCCA TGTGGGGACT TTTATCGCAC TGCAAAGTTC CAGATACACA AGAATACACA 
CGAAATGTTG TTCGATATTG CCTTGAAGCT CTTCAGGATT GGTTTGATGC TATCAATTTT 
GTAGATGAGC CTGCTCCAAA CCAGGTCACA TTTCACTTGC CTCTGCATCG ATACTATGCT 
ATGTTTTTAA GCAAGGCTAT AAAATGCCAA GAAATAGATT TAGATTCTAT TTTGCCTGAC 
CAGGAAATGC TGATGAAACT AATGGTTCAT CCACTACTAA TTCAGGCAAG TTTGTCTGAA 
ATACACAGCA ACATGTGGGT TCGGAATGGT CTCCAGATTA AAGGACAGGC TATGACATAT 
GTGCAGTCAC ATTTTTGCAA TTCCATGATC GACCCAGATA TCTATCTTCT ACAGGTCTGT 
GCTTCAAAAC TTGACCCAGA CTACTTTATT TCATCAGTCT TTGAAAGATT TAAAGTTGTA 
GATCTATTAA CGATGGCATC TCAGCACCAA AACACAGTGC TCGACGCAGA TCATGAACGG 
TCAATGCTTG AGGGAGCTTT AACATTTCTC GTCATTCTTT TAAGCCTCCG CTTGCATTTA 
GGAATGACAG ATGATGAGAT TCTCCGCGCA GAGATGATAT CCCAGCTCTG TATGAATGAC 
CGCACACACA GCTCACTCCT TGACCTTATT CCAGAAAGCC CAAACCCAAA AAGTGGTATT 
ATGCCAGGGA GTTGCAGCTT TGAATCTGTG TTGTCTGCAG TTGCTGATTT TAAAGCTCCC 
GTGTTTGAGC CTGGAGGCTC AATGCAACAA GGAATGTATA CTCCCAAGGG TGACATCTGG 
GATCAGGAAT TTGACCCCGT AATGGTGATT CTGAGGACAG TTTACAGGAG AGATGTACAG 
TCTGCCATGG ACAGATACAC AGCATTTTTA AAGCAGTCTG GGAGATTTTC TGGAAACCCA 
TGGCCGCCTT ACAAGAAAAG GAGCCCTCTG CATCACAGCT ATAAAGGCCT TATGAGGCTA 
TTGCACTGCA AAACATTGCA CATTGTGCTG TTTACACTGC TTTATAAGAT TCTGATGGAT 
CATCAGAATA TGTCAGAACA CGTTCTTTGT ATGGTTTTGT ATCTCATAGA GCTTGGGCTA 
GAAAATTCTC CCGAGGACGA GTCATGTGAA GAGAATTTTG CTGGGCCAGA TAGATGCCAC 
GACAGCTGGT TTCCTGGAAG TAACCTACTG TGCAACATGC GCCACTTTAT TAACTACGTC 
AGAGTGCGAG TACCAGAGAC TGCTCCAGAG GTGAGGAGGG AGCCCCCAGC AAGCACTAGC 
ACTGACGGAC CATCCTCGGC ACAGAATTCA GGTACAGCTC AGGTTTTCAG CTTGGTAGCA 
GAACGTCGGA AAAAGTTCCA GGAGATAATT AACCGCAATA ACAGTGAAGC CAATCAAGTT 
GTGCGCCCCA AATCTTCAGC TCTGAAGTGG ACGGCACCCG GTGCAACTCC TCAGTTAACG 
ACAGTCATAT TAGAGGTGAA AGAGAGCATG CTATCCCTGC TTATTAAACT GCACAAGAAG 
CTCTCGGGAA AGCAGGATTC CTATTGCCCC CCCTGGTTGG ATGATCCAGA ATGCCTTGTC 
CAGCCGGACT TACCTGGGTA TTGTCATGGC GATGGTGTAA CCGCTGTAGA ACGAATTTTA 
CTGAAAGCAG CATCACAAAG TCGTCTGAAC AGACAGAGTA TTGATGAGAT ATGCAAGAAA 
GCTCCTCCAC CCGTGCCACC CAAAAAATCT AGTCCTGCTG ACAAGAAGAC TTTGGGTAAA 
GAAGAAAGAC GGCAGAAAGC GAGGGAGAGG CAACAGAAAT TGCTTGCGGA ATTCGCCTCA 
AGGCAAAAAA GCTTCATGGA AACAGCGATG GATGTTGAAT CACCTGAAAG TGATGTGGCA 
ATGGAAATAG CTGGAGCTGA ACAGCACGTG GCAGAACCAA TATATGATTG TGTTATATGT 
GGACAGAGTG GCCCATCTAC AGAAGATCGC CCTCTAGGGC TTGTTGTTCT GCTTCAGGCA 
TCTTCTGTTT TGGGGCATCG GCGTAAGAGC ACAGAGCCAA AGAAACTACC AACCACAGAG 
GAGGAGCAGA TATACCAAGA GGGCACATGT GCAGCAGCTA ATGATGTCAA ACTTGCAATA 
TTACAGAGAT ATTTCAAAGA TAGCTCCTGT CTTCAGTCAG TATCAGTTGG TTGGGAAGGC 
GGCGTCTATG TTCAGACCTG TGGCCATACT CTGCATATTG ACTGCCATAA ATCTTATATG 
GAATCCTTAC GGAATGACCA GGTTTTACAA GGCTTTTCTG TAGACAAAGG GGAATTTACA 
TGCCCGCTGT GCAGGCAGTT TGCAAACAGC GTCCTTCCAT GCTTCCCGGG AGACAGTACA 
GAGAGGGGGT CGTGGCACAG CCATGCAGCC AGCAGCAAAA GTCTGCAGGA GCTCATCAAA 
GAGGTGGAGG ATCTTCAGGA GCAGCTGGGT ACTTTCCCAG TAAGCATCAG TTCAGAAACA 
AATTTAAGCA AGGAAATGGA GGCCGTTATG AAGGATATTA AAAGCACTAC GCAGAAGAAA 
TACACAGACT ATAGCAAGAC TCAAGGTTCC CCAGACAACG ACTTTCTCTT CATGTATTCA 
GTGGCAAGAA CTAATTTGGA ACTGGAGCTT ATACATTGTG GAGACAATCT TTGCTCTGGA 
GGGGCCAGTG CTGCCGGGAA AAGATCATGT CTGAATCAGC TTTTTCATGT ATTAGCTATG 
CACATGCGAT TATACAGCAT TGATCCCGAA TACAACCCCT GGAAAAAGTT AACCCAAGTC 
ATACAGAAAA AACCTTCCCT CGGAGACGAT GAGGAGAGAG AAGAGGTTCC GATACTTTTC 
CGAGATGTCA CCTCTCTTTT GCTCATTCAG GTTCTAACAA TGCCCCAGCC ATTGTGCAAA 
GAGCATTTTG TTTGCATTGT GAAAGTCCTC TACACGTTGC TATACACTCA AGCGATCACA 
TCCCTCTCGG TAAAACTCCA GGCTGAAGAT CGCAAGGTTT GGAGGGAATC TGGATCCAGC 
AAGAAGAGTT CATATTCTTC TGGAAAACCT TGGGAAGCTT TGCTTGGGTT TGTGATAAGT 
GAACTATCAA GAGGGAGCTT ATACCAAGAT GAAGAGACTG ACAGTATAAA CATGAGTGAC 
TTCACAGCTT GGTACGAAGA CGCCATTGAG CATTATTTAC AGCAGTTTTG CTTACCTTTC 
CTCAGGATTA CAAGCTTTCT CCAGCATCAC CTTTTCGGAT TAGATTTGCC AAGTTGCCAG 
GAAGAAGATG AATTTACTGT CCTTTCCAAC TGTTTGGGAC TTTTGCCATC TTTCAGTGAA 
GCATCGCACC AGTTTGTCAG TGCCTCCTTA CTTGACTGGC CAGTGTCCGC ATTTGATTTA 
ATATCTCAGT GGTGTTCAGA AGTCAATTTG TTTTCAGACA AGCACCCAGA CCAAGCTAAC 
GTTTTGCTTA TTCAAGATAC CAAGTGGGAC TTACCACGCC TCCTTCAGTT GCCAGAAAAC 
TACAATACAA TATTTCAATA TTATCATAGA AAGTGCTGCA GTGTCTGTAC AAAGGTTCCC 
AAGGATCCTG CACTTTGCCT AGTTTGTGGC ACATTTGTTT GCTTAAAGGG CCTCTGCTGC 
AAACAACAGA GTTACTGTGA ATGTGTATTG CACTCCCAAA ACTGTGGAGC CGGCACTGGA 
ATTTTCCTCT TGATCAACGC TTCTGTCATC ATTATTATTC GAGGCCATCG CTTCTGCCTT 
TGGGGCTCTG TATATCTCGA TGCACATGGG GAGGAAGACA GAGACCTTAG GCGCGGAAAG 
CCCCTGTACA TCTGTAAGGA AAGGTACAGC ATGCTGGAGC AGCAATGGGT CTCGCACACG 
TTTGATCACA TCAATAAGAG ATGGGGGCCG CATTACAATG GCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826538.1
CDS	196..5763
Translation	MMAAETPISRDKEATAALLKSKLSRPDQSPGLERLNGLLDRVLCVGGPAGEALEWCKCLLAGGDKFEDFCQAVRSYDNATLCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGEHAGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHRVKLSSCVPSVPQDLLVMSELVIPRFIFYLIQYLRDGYSEPGAEASPEKDLQKTLQMLDPQISFLEDLTKMGGAMRSVLTQVLTNQQLYKDLSSGPGESTCSKSHEKYLIALKESGLSIPEENVSSGAQEQSPGTSTLAVKEVAGAEGAQGQLESSDEEDQDGRQCVGKRKRVKLSSNTKDQSIMDMLKHKCFLEELLFWTIKYEFPQKMVTFLLNMLPDQDYKIAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKKFLEDHGLLVTWMNFVSFFQGMNLNKRELTEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVPDTQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAIKCQEIDLDSILPDQEMLMKLMVHPLLIQASLSEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASKLDPDYFISSVFERFKVVDLLTMASQHQNTVLDADHERSMLEGALTFLVILLSLRLHLGMTDDEILRAEMISQLCMNDRTHSSLLDLIPESPNPKSGIMPGSCSFESVLSAVADFKAPVFEPGGSMQQGMYTPKGDIWDQEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGRFSGNPWPPYKKRSPLHHSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNMSEHVLCMVLYLIELGLENSPEDESCEENFAGPDRCHDSWFPGSNLLCNMRHFINYVRVRVPETAPEVRREPPASTSTDGPSSAQNSGTAQVFSLVAERRKKFQEIINRNNSEANQVVRPKSSALKWTAPGATPQLTTVILEVKESMLSLLIKLHKKLSGKQDSYCPPWLDDPECLVQPDLPGYCHGDGVTAVERILLKAASQSRLNRQSIDEICKKAPPPVPPKKSSPADKKTLGKEERRQKARERQQKLLAEFASRQKSFMETAMDVESPESDVAMEIAGAEQHVAEPIYDCVICGQSGPSTEDRPLGLVVLLQASSVLGHRRKSTEPKKLPTTEEEQIYQEGTCAAANDVKLAILQRYFKDSSCLQSVSVGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCFPGDSTERGSWHSHAASSKSLQELIKEVEDLQEQLGTFPVSISSETNLSKEMEAVMKDIKSTTQKKYTDYSKTQGSPDNDFLFMYSVARTNLELELIHCGDNLCSGGASAAGKRSCLNQLFHVLAMHMRLYSIDPEYNPWKKLTQVIQKKPSLGDDEEREEVPILFRDVTSLLLIQVLTMPQPLCKEHFVCIVKVLYTLLYTQAITSLSVKLQAEDRKVWRESGSSKKSSYSSGKPWEALLGFVISELSRGSLYQDEETDSINMSDFTAWYEDAIEHYLQQFCLPFLRITSFLQHHLFGLDLPSCQEEDEFTVLSNCLGLLPSFSEASHQFVSASLLDWPVSAFDLISQWCSEVNLFSDKHPDQANVLLIQDTKWDLPRLLQLPENYNTIFQYYHRKCCSVCTKVPKDPALCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYSMLEQQWVSHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_012971084.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971084.2

ORF Insert Sequence:

ATGATGGCGG CGGAGACGCC GATAAGCCGG GATAAAGAAG CCACAGCCGC TTTGCTGAAG 
AGCAAACTGA GCCGGCCGGA CCAGAGCCCG GGGTTGGAGC GGCTGAACGG ATTGCTGGAC 
CGGGTTCTGT GCGTAGGAGG GCCGGCCGGG GAGGCCCTGG AGTGGTGCAA GTGCCTGCTG 
GCCGGGGGGG ACAAGTTCGA GGACTTCTGT CAGGCTGTGC GCTCCTATGA CAATGCCACC 
CTGTGCGGCC TGGTCTGGAC GGCGAACTTC GTGGCCTATC GGTGCCGGAC GTGCGGGATA 
TCCCCATGCA TGTCCCTGTG CGCAGAGTGC TTCCACCAGG GCGAACATGC CGGCCATGAC 
TTTAACATGT TCCGCAGCCA GGCCGGGGGG GCTTGTGACT GTGGGGACAG CAATGTCATG 
AGGGAGAGCG GGTTTTGTAA AAGACATAGA GTGAAGCTGA GCTCCTGTGT TCCCTCTGTG 
CCCCAAGACT TGTTGGTGAT GTCTGAATTG GTTATACCTC GTTTTATCTT TTATCTCATT 
CAGTATCTGA GAGATGGGTA TAGTGAACCA GGAGCTGAAG CATCGCCGGA AAAAGATCTA 
CAGAAAACCC TTCAGATGCT GGATCCTCAG ATCTCCTTCT TGGAAGATTT AACCAAGATG 
GGTGGAGCTA TGAGGTCTGT TCTCACCCAG GTCCTGACCA ACCAGCAGCT TTATAAAGAC 
CTTTCTTCTG GTCCTGGAGA GAGTACATGC TCTAAGAGCC ATGAAAAGTA TCTCATAGCT 
CTGAAGGAAT CTGGTTTGTC AATTCCAGAA GAGAATGTTT CCAGTGGGGC ACAGGAGCAG 
TCACCTGGCA CAAGTACACT AGCAGTTAAA GAGGTTGCAG GTGCAGAAGG TGCCCAAGGG 
CAGCTAGAAT CTTCAGATGA GGAGGACCAG GATGGCAGGC AGTGTGTTGG GAAAAGAAAA 
AGAGTGAAGC TAAGCAGTAA CACCAAAGAT CAATCTATCA TGGATATGTT GAAACACAAA 
TGTTTTCTAG AAGAACTGTT ATTTTGGACA ATAAAGTATG AATTTCCACA GAAAATGGTC 
ACTTTCTTAT TGAATATGTT ACCAGATCAA GATTATAAGA TTGCATTTAC GAAAACATTT 
GTCCAGCATT ATGCTTTCAT TATGAAGACG TTGAAGAAAA GCCATGAATC GGACACTATG 
TCCAATCGAA TTGTGCACAT CAGTGTCCAG CTGTTCAGCA ATGAGGAGCT GGCGCGTCAA 
GTCACAGAAG AGTGCCAACT TCTGGACATT ATGGTCACTG TGCTACTGTA CATGATGGAA 
AGTTGCCTTA TTAAAAGTGA ACTGCAAGAT GAAGAAAACA GTTTACATGT GGTGGTGAAT 
TGTGGAGAAG CTTTACTGAA AAACAATACC TACTGGCCCC TTGTTAGTGA TTTTATTAAT 
ATCCTCTCTC ACCAAAGTGT AGCTAAGAAA TTCCTAGAGG ATCACGGGCT ATTAGTTACT 
TGGATGAACT TTGTATCATT CTTTCAAGGT ATGAATTTAA ACAAAAGAGA GCTCACCGAA 
CATGTAGAAT TTGAATCCCA GACATACTAT GCTGCATTTG CTGCTGAACT TGAAGCCTGT 
GCCCAGCCCA TGTGGGGACT TTTATCGCAC TGCAAAGTTC CAGATACACA AGAATACACA 
CGAAATGTTG TTCGATATTG CCTTGAAGCT CTTCAGGATT GGTTTGATGC TATCAATTTT 
GTAGATGAGC CTGCTCCAAA CCAGGTCACA TTTCACTTGC CTCTGCATCG ATACTATGCT 
ATGTTTTTAA GCAAGGCTAT AAAATGCCAA GAAATAGATT TAGATTCTAT TTTGCCTGAC 
CAGGAAATGC TGATGAAACT AATGGTTCAT CCACTACTAA TTCAGGCAAG TTTGTCTGAA 
ATACACAGCA ACATGTGGGT TCGGAATGGT CTCCAGATTA AAGGACAGGC TATGACATAT 
GTGCAGTCAC ATTTTTGCAA TTCCATGATC GACCCAGATA TCTATCTTCT ACAGGTCTGT 
GCTTCAAAAC TTGACCCAGA CTACTTTATT TCATCAGTCT TTGAAAGATT TAAAGTTGTA 
GATCTATTAA CGATGGCATC TCAGCACCAA AACACAGTGC TCGACGCAGA TCATGAACGG 
TCAATGCTTG AGGGAGCTTT AACATTTCTC GTCATTCTTT TAAGCCTCCG CTTGCATTTA 
GGAATGACAG ATGATGAGAT TCTCCGCGCA GAGATGATAT CCCAGCTCTG TATGAATGAC 
CGCACACACA GCTCACTCCT TGACCTTATT CCAGAAAGCC CAAACCCAAA AAGTGGTATT 
ATGCCAGGGA GTTGCAGCTT TGAATCTGTG TTGTCTGCAG TTGCTGATTT TAAAGCTCCC 
GTGTTTGAGC CTGGAGGCTC AATGCAACAA GGAATGTATA CTCCCAAGGG TGACATCTGG 
GATCAGGAAT TTGACCCCGT AATGGTGATT CTGAGGACAG TTTACAGGAG AGATGTACAG 
TCTGCCATGG ACAGATACAC AGCATTTTTA AAGCAGTCTG GGAGATTTTC TGGAAACCCA 
TGGCCGCCTT ACAAGAAAAG GAGCCCTCTG CATCACAGCT ATAAAGGCCT TATGAGGCTA 
TTGCACTGCA AAACATTGCA CATTGTGCTG TTTACACTGC TTTATAAGAT TCTGATGGAT 
CATCAGAATA TGTCAGAACA CGTTCTTTGT ATGGTTTTGT ATCTCATAGA GCTTGGGCTA 
GAAAATTCTC CCGAGGACGA GTCATGTGAA GAGAATTTTG CTGGGCCAGA TAGATGCCAC 
GACAGCTGGT TTCCTGGAAG TAACCTACTG TGCAACATGC GCCACTTTAT TAACTACGTC 
AGAGTGCGAG TACCAGAGAC TGCTCCAGAG GTGAGGAGGG AGCCCCCAGC AAGCACTAGC 
ACTGACGGAC CATCCTCGGC ACAGAATTCA GGTACAGCTC AGGTTTTCAG CTTGGTAGCA 
GAACGTCGGA AAAAGTTCCA GGAGATAATT AACCGCAATA ACAGTGAAGC CAATCAAGTT 
GTGCGCCCCA AATCTTCAGC TCTGAAGTGG ACGGCACCCG GTGCAACTCC TCAGTTAACG 
ACAGTCATAT TAGAGGTGAA AGAGAGCATG CTATCCCTGC TTATTAAACT GCACAAGAAG 
CTCTCGGGAA AGCAGGATTC CTATTGCCCC CCCTGGTTGG ATGATCCAGA ATGCCTTGTC 
CAGCCGGACT TACCTGGGTA TTGTCATGGC GATGGTGTAA CCGCTGTAGA ACGAATTTTA 
CTGAAAGCAG CATCACAAAG TCGTCTGAAC AGACAGAGTA TTGATGAGAT ATGCAAGAAA 
GCTCCTCCAC CCGTGCCACC CAAAAAATCT AGTCCTGCTG ACAAGAAGAC TTTGGGTAAA 
GAAGAAAGAC GGCAGAAAGC GAGGGAGAGG CAACAGAAAT TGCTTGCGGA ATTCGCCTCA 
AGGCAAAAAA GCTTCATGGA AACAGCGATG GATGTTGAAT CACCTGAAAG TGATGTGGCA 
ATGGAAATAG CTGGAGCTGA ACAGCACGTG GCAGAACCAA TATATGATTG TGTTATATGT 
GGACAGAGTG GCCCATCTAC AGAAGATCGC CCTCTAGGGC TTGTTGTTCT GCTTCAGGCA 
TCTTCTGTTT TGGGGCATCG GCGTAAGAGC ACAGAGCCAA AGAAACTACC AACCACAGAG 
GAGGAGCAGA TATACCAAGA GGGCACATGT GCAGCAGCTA ATGATGTCAA ACTTGCAATA 
TTACAGAGAT ATTTCAAAGA TAGCTCCTGT CTTCAGTCAG TATCAGTTGG TTGGGAAGGC 
GGCGTCTATG TTCAGACCTG TGGCCATACT CTGCATATTG ACTGCCATAA ATCTTATATG 
GAATCCTTAC GGAATGACCA GGTTTTACAA GGCTTTTCTG TAGACAAAGG GGAATTTACA 
TGCCCGCTGT GCAGGCAGTT TGCAAACAGC GTCCTTCCAT GCTTCCCGGG AGACAGTACA 
GAGAGGGGGT CGTGGCACAG CCATGCAGCC AGCAGCAAAA GTCTGCAGGA GCTCATCAAA 
GAGGTGGAGG ATCTTCAGGA GCAGCTGGGT ACTTTCCCAG TAAGCATCAG TTCAGAAACA 
AATTTAAGCA AGGAAATGGA GGCCGTTATG AAGGATATTA AAAGCACTAC GCAGAAGAAA 
TACACAGACT ATAGCAAGAC TCAAGGTTCC CCAGACAACG ACTTTCTCTT CATGTATTCA 
GTGGCAAGAA CTAATTTGGA ACTGGAGCTT ATACATTGTG GAGACAATCT TTGCTCTGGA 
GGGGCCAGTG CTGCCGGGAA AAGATCATGT CTGAATCAGC TTTTTCATGT ATTAGCTATG 
CACATGCGAT TATACAGCAT TGATCCCGAA TACAACCCCT GGAAAAAGTT AACCCAAGTC 
ATACAGAAAA AACCTTCCCT CGGAGACGAT GAGGAGAGAG AAGAGGTTCC GATACTTTTC 
CGAGATGTCA CCTCTCTTTT GCTCATTCAG GTTCTAACAA TGCCCCAGCC ATTGTGCAAA 
GAGCATTTTG TTTGCATTGT GAAAGTCCTC TACACGTTGC TATACACTCA AGCGATCACA 
TCCCTCTCGG TAAAACTCCA GGCTGAAGAT CGCAAGGTTT GGAGGGAATC TGGATCCAGC 
AAGAAGAGTT CATATTCTTC TGGAAAACCT TGGGAAGCTT TGCTTGGGTT TGTGATAAGT 
GAACTATCAA GAGGGAGCTT ATACCAAGAT GAAGAGACTG ACAGTATAAA CATGAGTGAC 
TTCACAGCTT GGTACGAAGA CGCCATTGAG CATTATTTAC AGCAGTTTTG CTTACCTTTC 
CTCAGGATTA CAAGCTTTCT CCAGCATCAC CTTTTCGGAT TAGATTTGCC AAGTTGCCAG 
GAAGAAGATG AATTTACTGT CCTTTCCAAC TGTTTGGGAC TTTTGCCATC TTTCAGTGAA 
GCATCGCACC AGTTTGTCAG TGCCTCCTTA CTTGACTGGC CAGTGTCCGC ATTTGATTTA 
ATATCTCAGT GGTGTTCAGA AGTCAATTTG TTTTCAGACA AGCACCCAGA CCAAGCTAAC 
GTTTTGCTTA TTCAAGATAC CAAGTGGGAC TTACCACGCC TCCTTCAGTT GCCAGAAAAC 
TACAATACAA TATTTCAATA TTATCATAGA AAGTGCTGCA GTGTCTGTAC AAAGGTTCCC 
AAGGATCCTG CACTTTGCCT AGTTTGTGGC ACATTTGTTT GCTTAAAGGG CCTCTGCTGC 
AAACAACAGA GTTACTGTGA ATGTGTATTG CACTCCCAAA ACTGTGGAGC CGGCACTGGA 
ATTTTCCTCT TGATCAACGC TTCTGTCATC ATTATTATTC GAGGCCATCG CTTCTGCCTT 
TGGGGCTCTG TATATCTCGA TGCACATGGG GAGGAAGACA GAGACCTTAG GCGCGGAAAG 
CCCCTGTACA TCTGTAAGGA AAGGTACAGC ATGCTGGAGC AGCAATGGGT CTCGCACACG 
TTTGATCACA TCAATAAGAG ATGGGGGCCG CATTACAATG GCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr3 ( XM_012971085.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826539.1 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X4, mRNA.

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CloneID	OXa37229
Clone ID Related Accession (Same CDS sequence)	XM_012971085.2 , XM_012971085.3
Accession Version	XM_012971085.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971085.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CGGAGACGCC GATAAGCCGG GATAAAGAAG CCACAGCCGC TTTGCTGAAG 
AGCAAACTGA GCCGGCCGGA CCAGAGCCCG GGGTTGGAGC GGCTGAACGG ATTGCTGGAC 
CGGGTTCTGT GCGTAGGAGG GCCGGCCGGG GAGGCCCTGG AGTGGTGCAA GTGCCTGCTG 
GCCGGGGGGG ACAAGTTCGA GGACTTCTGT CAGGCTGTGC GCTCCTATGA CAATGCCACC 
CTGTGCGGCC TGGTCTGGAC GGCGAACTTC GTGGCCTATC GGTGCCGGAC GTGCGGGATA 
TCCCCATGCA TGTCCCTGTG CGCAGAGTGC TTCCACCAGG GCGAACATGC CGGCCATGAC 
TTTAACATGT TCCGCAGCCA GGCCGGGGGG GCTTGTGACT GTGGGGACAG CAATGTCATG 
AGGGAGAGCG GGTTTTGTAA AAGACATAGA GTGAAGCTGA GCTCCTGTGT TCCCTCTGTG 
CCCCAAGACT TGTTGGTGAT GTCTGAATTG GTTATACCTC GTTTTATCTT TTATCTCATT 
CAGTATCTGA GAGATGGGTA TAGTGAACCA GGAGCTGAAG CATCGCCGGA AAAAGATCTA 
CAGAAAACCC TTCAGATGCT GGATCCTCAG ATCTCCTTCT TGGAAGATTT AACCAAGATG 
GGTGGAGCTA TGAGGTCTGT TCTCACCCAG GTCCTGACCA ACCAGCAGCT TTATAAAGAC 
CTTTCTTCTG GTCCTGGAGA GAGTACATGC TCTAAGAGCC ATGAAAAGTA TCTCATAGCT 
CTGAAGGAAT CTGGTTTGTC AATTCCAGAA GAGAATGTTT CCAGTGGGGC ACAGGAGCAG 
TCACCTGGCA CAAGTACACT AGCAGTTAAA GAGGTTGCAG GTGCAGAAGG TGCCCAAGGG 
CAGCTAGAAT CTTCAGATGA GGAGGACCAG GATGGCAGGC AGTGTGTTGG GAAAAGAAAA 
AGAGTGAAGC TAAGCAGTAA CACCAAAGAT CAATCTATCA TGGATATGTT GAAACACAAA 
TGTTTTCTAG AAGAACTGTT ATTTTGGACA ATAAAGTATG AATTTCCACA GAAAATGGTC 
ACTTTCTTAT TGAATATGTT ACCAGATCAA GATTATAAGA TTGCATTTAC GAAAACATTT 
GTCCAGCATT ATGCTTTCAT TATGAAGACG TTGAAGAAAA GCCATGAATC GGACACTATG 
TCCAATCGAA TTGTGCACAT CAGTGTCCAG CTGTTCAGCA ATGAGGAGCT GGCGCGTCAA 
GTCACAGAAG AGTGCCAACT TCTGGACATT ATGGTCACTG TGCTACTGTA CATGATGGAA 
AGTTGCCTTA TTAAAAGTGA ACTGCAAGAT GAAGAAAACA GTTTACATGT GGTGGTGAAT 
TGTGGAGAAG CTTTACTGAA AAACAATACC TACTGGCCCC TTGTTAGTGA TTTTATTAAT 
ATCCTCTCTC ACCAAAGTGT AGCTAAGAAA TTCCTAGAGG ATCACGGGCT ATTAGTTACT 
TGGATGAACT TTGTATCATT CTTTCAAGGT ATGAATTTAA ACAAAAGAGA GCTCACCGAA 
CATGTAGAAT TTGAATCCCA GACATACTAT GCTGCATTTG CTGCTGAACT TGAAGCCTGT 
GCCCAGCCCA TGTGGGGACT TTTATCGCAC TGCAAAGTTC CAGATACACA AGAATACACA 
CGAAATGTTG TTCGATATTG CCTTGAAGCT CTTCAGGATT GGTTTGATGC TATCAATTTT 
GTAGATGAGC CTGCTCCAAA CCAGGTCACA TTTCACTTGC CTCTGCATCG ATACTATGCT 
ATGTTTTTAA GCAAGGCTAT AAAATGCCAA GAAATAGATT TAGATTCTAT TTTGCCTGAC 
CAGGAAATGC TGATGAAACT AATGGTTCAT CCACTACTAA TTCAGGCAAG TTTGTCTGAA 
ATACACAGCA ACATGTGGGT TCGGAATGGT CTCCAGATTA AAGGACAGGC TATGACATAT 
GTGCAGTCAC ATTTTTGCAA TTCCATGATC GACCCAGATA TCTATCTTCT ACAGGTCTGT 
GCTTCAAAAC TTGACCCAGA CTACTTTATT TCATCAGTCT TTGAAAGATT TAAAGTTGTA 
GATCTATTAA CGATGGCATC TCAGCACCAA AACACAGTGC TCGACGCAGA TCATGAACGG 
TCAATGCTTG AGGGAGCTTT AACATTTCTC GTCATTCTTT TAAGCCTCCG CTTGCATTTA 
GGAATGACAG ATGATGAGAT TCTCCGCGCA GAGATGATAT CCCAGCTCTG TATGAATGAC 
CGCACACACA GCTCACTCCT TGACCTTATT CCAGAAAGCC CAAACCCAAA AAGTGGTATT 
ATGCCAGGGA GTTGCAGCTT TGAATCTGTG TTGTCTGCAG TTGCTGATTT TAAAGCTCCC 
GTGTTTGAGC CTGGAGGCTC AATGCAACAA GGAATGTATA CTCCCAAGGG TGACATCTGG 
GATCAGGAAT TTGACCCCGT AATGGTGATT CTGAGGACAG TTTACAGGAG AGATGTACAG 
TCTGCCATGG ACAGATACAC AGCATTTTTA AAGCAGTCTG GGAGATTTTC TGGAAACCCA 
TGGCCGCCTT ACAAGAAAAG GAGCCCTCTG CATCACAGCT ATAAAGGCCT TATGAGGCTA 
TTGCACTGCA AAACATTGCA CATTGTGCTG TTTACACTGC TTTATAAGAT TCTGATGGAT 
CATCAGAATA TGTCAGAACA CGTTCTTTGT ATGGTTTTGT ATCTCATAGA GCTTGGGCTA 
GAAAATTCTC CCGAGGACGA GTCATGTGAA GAGAATTTTG CTGGGCCAGA TAGATGCCAC 
GACAGCTGGT TTCCTGGAAG TAACCTACTG TGCAACATGC GCCACTTTAT TAACTACGTC 
AGAGTGCGAG TACCAGAGAC TGCTCCAGAG GTGAGGAGGG AGCCCCCAGC AAGCACTAGC 
ACTGACGGAC CATCCTCGGC ACAGAATTCA GGTACAGCTC AGGTTTTCAG CTTGGTAGCA 
GAACGTCGGA AAAAGTTCCA GGAGATAATT AACCGCAATA ACAGTGAAGC CAATCAAGTT 
GTGCGCCCCA AATCTTCAGC TCTGAAGTGG ACGGCACCCG GTGCAACTCC TCAGTTAACG 
ACAGTCATAT TAGAGGTGAA AGAGAGCATG CTATCCCTGC TTATTAAACT GCACAAGAAG 
CTCTCGGGAA AGCAGGATTC CTATTGCCCC CCCTGGTTGG ATGATCCAGA ATGCCTTGTC 
CAGCCGGACT TACCTGGGTA TTGTCATGGC GATGGTGTAA CCGCTGTAGA ACGAATTTTA 
CTGAAAGCAG CATCACAAAG TCGTCTGAAC AGACAGAGTA TTGATGAGAT ATGCAAGAAA 
GCTCCTCCAC CCGTGCCACC CAAAAAATCT AGTCCTGCTG ACAAGAAGAC TTTGGGTAAA 
GAAGAAAGAC GGCAGAAAGC GAGGGAGAGG CAACAGAAAT TGCTTGCGGA ATTCGCCTCA 
AGGCAAAAAA GCTTCATGGA AACAGCGATG GATGTTGAAT CACCTGAAAG TGATGTGGCA 
ATGGAAATAG CTGGAGCTGA ACAGCACGTG GCAGAACCAA TATATGATTG TGTTATATGT 
GGACAGAGTG GCCCATCTAC AGAAGATCGC CCTCTAGGGC TTGTTGTTCT GCTTCAGGCA 
TCTTCTGTTT TGGGGCATCG GCGTAAGAGC ACAGAGCCAA AGAAACTACC AACCACAGAG 
GAGGAGCAGA TATACCAAGA GGGCACATGT GCAGCAGCTA ATGATGTCAA ACTTGCAATA 
TTACAGAGAT ATTTCAAAGA TAGCTCCTGT CTTCAGTCAG TATCAGTTGG TTGGGAAGGC 
GGCGTCTATG TTCAGACCTG TGGCCATACT CTGCATATTG ACTGCCATAA ATCTTATATG 
GAATCCTTAC GGAATGACCA GGTTTTACAA GGCTTTTCTG TAGACAAAGG GGAATTTACA 
TGCCCGCTGT GCAGGCAGTT TGCAAACAGC GTCCTTCCAT GCTTCCCGGG AGACAGTACA 
GAGAGGGGGT CGTGGCACAG CCATGCAGCC AGCAGCAAAA GTCTGCAGGA GCTCATCAAA 
GAGGTGGAGG ATCTTCAGGA GCAGCTGGGT ACTTTCCCAT CAGAAACAAA TTTAAGCAAG 
GAAATGGAGG CCGTTATGAA GGATATTAAA AGCACTACGC AGAAGAAATA CACAGACTAT 
AGCAAGACTC AAGGTTCCCC AGACAACGAC TTTCTCTTCA TGTATTCAGT GGCAAGAACT 
AATTTGGAAC TGGAGCTTAT ACATTGTGGA GACAATCTTT GCTCTGGAGG GGCCAGTGCT 
GCCGGGAAAA GATCATGTCT GAATCAGCTT TTTCATGTAT TAGCTATGCA CATGCGATTA 
TACAGCATTG ATCCCGAATA CAACCCCTGG AAAAAGTTAA CCCAAGTCAT ACAGAAAAAA 
CCTTCCCTCG GAGACGATGA GGAGAGAGAA GAGGTTCCGA TACTTTTCCG AGATGTCACC 
TCTCTTTTGC TCATTCAGGT TCTAACAATG CCCCAGCCAT TGTGCAAAGA GCATTTTGTT 
TGCATTGTGA AAGTCCTCTA CACGTTGCTA TACACTCAAG CGATCACATC CCTCTCGGTA 
AAACTCCAGG CTGAAGATCG CAAGGTTTGG AGGGAATCTG GATCCAGCAA GAAGAGTTCA 
TATTCTTCTG GAAAACCTTG GGAAGCTTTG CTTGGGTTTG TGATAAGTGA ACTATCAAGA 
GGGAGCTTAT ACCAAGATGA AGAGACTGAC AGTATAAACA TGAGTGACTT CACAGCTTGG 
TACGAAGACG CCATTGAGCA TTATTTACAG CAGTTTTGCT TACCTTTCCT CAGGATTACA 
AGCTTTCTCC AGCATCACCT TTTCGGATTA GATTTGCCAA GTTGCCAGGA AGAAGATGAA 
TTTACTGTCC TTTCCAACTG TTTGGGACTT TTGCCATCTT TCAGTGAAGC ATCGCACCAG 
TTTGTCAGTG CCTCCTTACT TGACTGGCCA GTGTCCGCAT TTGATTTAAT ATCTCAGTGG 
TGTTCAGAAG TCAATTTGTT TTCAGACAAG CACCCAGACC AAGCTAACGT TTTGCTTATT 
CAAGATACCA AGTGGGACTT ACCACGCCTC CTTCAGTTGC CAGAAAACTA CAATACAATA 
TTTCAATATT ATCATAGAAA GTGCTGCAGT GTCTGTACAA AGGTTCCCAA GGATCCTGCA 
CTTTGCCTAG TTTGTGGCAC ATTTGTTTGC TTAAAGGGCC TCTGCTGCAA ACAACAGAGT 
TACTGTGAAT GTGTATTGCA CTCCCAAAAC TGTGGAGCCG GCACTGGAAT TTTCCTCTTG 
ATCAACGCTT CTGTCATCAT TATTATTCGA GGCCATCGCT TCTGCCTTTG GGGCTCTGTA 
TATCTCGATG CACATGGGGA GGAAGACAGA GACCTTAGGC GCGGAAAGCC CCTGTACATC 
TGTAAGGAAA GGTACAGCAT GCTGGAGCAG CAATGGGTCT CGCACACGTT TGATCACATC 
AATAAGAGAT GGGGGCCGCA TTACAATGGC TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826539.1
CDS	197..5752
Translation	MMAAETPISRDKEATAALLKSKLSRPDQSPGLERLNGLLDRVLCVGGPAGEALEWCKCLLAGGDKFEDFCQAVRSYDNATLCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGEHAGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHRVKLSSCVPSVPQDLLVMSELVIPRFIFYLIQYLRDGYSEPGAEASPEKDLQKTLQMLDPQISFLEDLTKMGGAMRSVLTQVLTNQQLYKDLSSGPGESTCSKSHEKYLIALKESGLSIPEENVSSGAQEQSPGTSTLAVKEVAGAEGAQGQLESSDEEDQDGRQCVGKRKRVKLSSNTKDQSIMDMLKHKCFLEELLFWTIKYEFPQKMVTFLLNMLPDQDYKIAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKKFLEDHGLLVTWMNFVSFFQGMNLNKRELTEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVPDTQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAIKCQEIDLDSILPDQEMLMKLMVHPLLIQASLSEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASKLDPDYFISSVFERFKVVDLLTMASQHQNTVLDADHERSMLEGALTFLVILLSLRLHLGMTDDEILRAEMISQLCMNDRTHSSLLDLIPESPNPKSGIMPGSCSFESVLSAVADFKAPVFEPGGSMQQGMYTPKGDIWDQEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGRFSGNPWPPYKKRSPLHHSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNMSEHVLCMVLYLIELGLENSPEDESCEENFAGPDRCHDSWFPGSNLLCNMRHFINYVRVRVPETAPEVRREPPASTSTDGPSSAQNSGTAQVFSLVAERRKKFQEIINRNNSEANQVVRPKSSALKWTAPGATPQLTTVILEVKESMLSLLIKLHKKLSGKQDSYCPPWLDDPECLVQPDLPGYCHGDGVTAVERILLKAASQSRLNRQSIDEICKKAPPPVPPKKSSPADKKTLGKEERRQKARERQQKLLAEFASRQKSFMETAMDVESPESDVAMEIAGAEQHVAEPIYDCVICGQSGPSTEDRPLGLVVLLQASSVLGHRRKSTEPKKLPTTEEEQIYQEGTCAAANDVKLAILQRYFKDSSCLQSVSVGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCFPGDSTERGSWHSHAASSKSLQELIKEVEDLQEQLGTFPSETNLSKEMEAVMKDIKSTTQKKYTDYSKTQGSPDNDFLFMYSVARTNLELELIHCGDNLCSGGASAAGKRSCLNQLFHVLAMHMRLYSIDPEYNPWKKLTQVIQKKPSLGDDEEREEVPILFRDVTSLLLIQVLTMPQPLCKEHFVCIVKVLYTLLYTQAITSLSVKLQAEDRKVWRESGSSKKSSYSSGKPWEALLGFVISELSRGSLYQDEETDSINMSDFTAWYEDAIEHYLQQFCLPFLRITSFLQHHLFGLDLPSCQEEDEFTVLSNCLGLLPSFSEASHQFVSASLLDWPVSAFDLISQWCSEVNLFSDKHPDQANVLLIQDTKWDLPRLLQLPENYNTIFQYYHRKCCSVCTKVPKDPALCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYSMLEQQWVSHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_012971085.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971085.2

ORF Insert Sequence:

ATGATGGCGG CGGAGACGCC GATAAGCCGG GATAAAGAAG CCACAGCCGC TTTGCTGAAG 
AGCAAACTGA GCCGGCCGGA CCAGAGCCCG GGGTTGGAGC GGCTGAACGG ATTGCTGGAC 
CGGGTTCTGT GCGTAGGAGG GCCGGCCGGG GAGGCCCTGG AGTGGTGCAA GTGCCTGCTG 
GCCGGGGGGG ACAAGTTCGA GGACTTCTGT CAGGCTGTGC GCTCCTATGA CAATGCCACC 
CTGTGCGGCC TGGTCTGGAC GGCGAACTTC GTGGCCTATC GGTGCCGGAC GTGCGGGATA 
TCCCCATGCA TGTCCCTGTG CGCAGAGTGC TTCCACCAGG GCGAACATGC CGGCCATGAC 
TTTAACATGT TCCGCAGCCA GGCCGGGGGG GCTTGTGACT GTGGGGACAG CAATGTCATG 
AGGGAGAGCG GGTTTTGTAA AAGACATAGA GTGAAGCTGA GCTCCTGTGT TCCCTCTGTG 
CCCCAAGACT TGTTGGTGAT GTCTGAATTG GTTATACCTC GTTTTATCTT TTATCTCATT 
CAGTATCTGA GAGATGGGTA TAGTGAACCA GGAGCTGAAG CATCGCCGGA AAAAGATCTA 
CAGAAAACCC TTCAGATGCT GGATCCTCAG ATCTCCTTCT TGGAAGATTT AACCAAGATG 
GGTGGAGCTA TGAGGTCTGT TCTCACCCAG GTCCTGACCA ACCAGCAGCT TTATAAAGAC 
CTTTCTTCTG GTCCTGGAGA GAGTACATGC TCTAAGAGCC ATGAAAAGTA TCTCATAGCT 
CTGAAGGAAT CTGGTTTGTC AATTCCAGAA GAGAATGTTT CCAGTGGGGC ACAGGAGCAG 
TCACCTGGCA CAAGTACACT AGCAGTTAAA GAGGTTGCAG GTGCAGAAGG TGCCCAAGGG 
CAGCTAGAAT CTTCAGATGA GGAGGACCAG GATGGCAGGC AGTGTGTTGG GAAAAGAAAA 
AGAGTGAAGC TAAGCAGTAA CACCAAAGAT CAATCTATCA TGGATATGTT GAAACACAAA 
TGTTTTCTAG AAGAACTGTT ATTTTGGACA ATAAAGTATG AATTTCCACA GAAAATGGTC 
ACTTTCTTAT TGAATATGTT ACCAGATCAA GATTATAAGA TTGCATTTAC GAAAACATTT 
GTCCAGCATT ATGCTTTCAT TATGAAGACG TTGAAGAAAA GCCATGAATC GGACACTATG 
TCCAATCGAA TTGTGCACAT CAGTGTCCAG CTGTTCAGCA ATGAGGAGCT GGCGCGTCAA 
GTCACAGAAG AGTGCCAACT TCTGGACATT ATGGTCACTG TGCTACTGTA CATGATGGAA 
AGTTGCCTTA TTAAAAGTGA ACTGCAAGAT GAAGAAAACA GTTTACATGT GGTGGTGAAT 
TGTGGAGAAG CTTTACTGAA AAACAATACC TACTGGCCCC TTGTTAGTGA TTTTATTAAT 
ATCCTCTCTC ACCAAAGTGT AGCTAAGAAA TTCCTAGAGG ATCACGGGCT ATTAGTTACT 
TGGATGAACT TTGTATCATT CTTTCAAGGT ATGAATTTAA ACAAAAGAGA GCTCACCGAA 
CATGTAGAAT TTGAATCCCA GACATACTAT GCTGCATTTG CTGCTGAACT TGAAGCCTGT 
GCCCAGCCCA TGTGGGGACT TTTATCGCAC TGCAAAGTTC CAGATACACA AGAATACACA 
CGAAATGTTG TTCGATATTG CCTTGAAGCT CTTCAGGATT GGTTTGATGC TATCAATTTT 
GTAGATGAGC CTGCTCCAAA CCAGGTCACA TTTCACTTGC CTCTGCATCG ATACTATGCT 
ATGTTTTTAA GCAAGGCTAT AAAATGCCAA GAAATAGATT TAGATTCTAT TTTGCCTGAC 
CAGGAAATGC TGATGAAACT AATGGTTCAT CCACTACTAA TTCAGGCAAG TTTGTCTGAA 
ATACACAGCA ACATGTGGGT TCGGAATGGT CTCCAGATTA AAGGACAGGC TATGACATAT 
GTGCAGTCAC ATTTTTGCAA TTCCATGATC GACCCAGATA TCTATCTTCT ACAGGTCTGT 
GCTTCAAAAC TTGACCCAGA CTACTTTATT TCATCAGTCT TTGAAAGATT TAAAGTTGTA 
GATCTATTAA CGATGGCATC TCAGCACCAA AACACAGTGC TCGACGCAGA TCATGAACGG 
TCAATGCTTG AGGGAGCTTT AACATTTCTC GTCATTCTTT TAAGCCTCCG CTTGCATTTA 
GGAATGACAG ATGATGAGAT TCTCCGCGCA GAGATGATAT CCCAGCTCTG TATGAATGAC 
CGCACACACA GCTCACTCCT TGACCTTATT CCAGAAAGCC CAAACCCAAA AAGTGGTATT 
ATGCCAGGGA GTTGCAGCTT TGAATCTGTG TTGTCTGCAG TTGCTGATTT TAAAGCTCCC 
GTGTTTGAGC CTGGAGGCTC AATGCAACAA GGAATGTATA CTCCCAAGGG TGACATCTGG 
GATCAGGAAT TTGACCCCGT AATGGTGATT CTGAGGACAG TTTACAGGAG AGATGTACAG 
TCTGCCATGG ACAGATACAC AGCATTTTTA AAGCAGTCTG GGAGATTTTC TGGAAACCCA 
TGGCCGCCTT ACAAGAAAAG GAGCCCTCTG CATCACAGCT ATAAAGGCCT TATGAGGCTA 
TTGCACTGCA AAACATTGCA CATTGTGCTG TTTACACTGC TTTATAAGAT TCTGATGGAT 
CATCAGAATA TGTCAGAACA CGTTCTTTGT ATGGTTTTGT ATCTCATAGA GCTTGGGCTA 
GAAAATTCTC CCGAGGACGA GTCATGTGAA GAGAATTTTG CTGGGCCAGA TAGATGCCAC 
GACAGCTGGT TTCCTGGAAG TAACCTACTG TGCAACATGC GCCACTTTAT TAACTACGTC 
AGAGTGCGAG TACCAGAGAC TGCTCCAGAG GTGAGGAGGG AGCCCCCAGC AAGCACTAGC 
ACTGACGGAC CATCCTCGGC ACAGAATTCA GGTACAGCTC AGGTTTTCAG CTTGGTAGCA 
GAACGTCGGA AAAAGTTCCA GGAGATAATT AACCGCAATA ACAGTGAAGC CAATCAAGTT 
GTGCGCCCCA AATCTTCAGC TCTGAAGTGG ACGGCACCCG GTGCAACTCC TCAGTTAACG 
ACAGTCATAT TAGAGGTGAA AGAGAGCATG CTATCCCTGC TTATTAAACT GCACAAGAAG 
CTCTCGGGAA AGCAGGATTC CTATTGCCCC CCCTGGTTGG ATGATCCAGA ATGCCTTGTC 
CAGCCGGACT TACCTGGGTA TTGTCATGGC GATGGTGTAA CCGCTGTAGA ACGAATTTTA 
CTGAAAGCAG CATCACAAAG TCGTCTGAAC AGACAGAGTA TTGATGAGAT ATGCAAGAAA 
GCTCCTCCAC CCGTGCCACC CAAAAAATCT AGTCCTGCTG ACAAGAAGAC TTTGGGTAAA 
GAAGAAAGAC GGCAGAAAGC GAGGGAGAGG CAACAGAAAT TGCTTGCGGA ATTCGCCTCA 
AGGCAAAAAA GCTTCATGGA AACAGCGATG GATGTTGAAT CACCTGAAAG TGATGTGGCA 
ATGGAAATAG CTGGAGCTGA ACAGCACGTG GCAGAACCAA TATATGATTG TGTTATATGT 
GGACAGAGTG GCCCATCTAC AGAAGATCGC CCTCTAGGGC TTGTTGTTCT GCTTCAGGCA 
TCTTCTGTTT TGGGGCATCG GCGTAAGAGC ACAGAGCCAA AGAAACTACC AACCACAGAG 
GAGGAGCAGA TATACCAAGA GGGCACATGT GCAGCAGCTA ATGATGTCAA ACTTGCAATA 
TTACAGAGAT ATTTCAAAGA TAGCTCCTGT CTTCAGTCAG TATCAGTTGG TTGGGAAGGC 
GGCGTCTATG TTCAGACCTG TGGCCATACT CTGCATATTG ACTGCCATAA ATCTTATATG 
GAATCCTTAC GGAATGACCA GGTTTTACAA GGCTTTTCTG TAGACAAAGG GGAATTTACA 
TGCCCGCTGT GCAGGCAGTT TGCAAACAGC GTCCTTCCAT GCTTCCCGGG AGACAGTACA 
GAGAGGGGGT CGTGGCACAG CCATGCAGCC AGCAGCAAAA GTCTGCAGGA GCTCATCAAA 
GAGGTGGAGG ATCTTCAGGA GCAGCTGGGT ACTTTCCCAT CAGAAACAAA TTTAAGCAAG 
GAAATGGAGG CCGTTATGAA GGATATTAAA AGCACTACGC AGAAGAAATA CACAGACTAT 
AGCAAGACTC AAGGTTCCCC AGACAACGAC TTTCTCTTCA TGTATTCAGT GGCAAGAACT 
AATTTGGAAC TGGAGCTTAT ACATTGTGGA GACAATCTTT GCTCTGGAGG GGCCAGTGCT 
GCCGGGAAAA GATCATGTCT GAATCAGCTT TTTCATGTAT TAGCTATGCA CATGCGATTA 
TACAGCATTG ATCCCGAATA CAACCCCTGG AAAAAGTTAA CCCAAGTCAT ACAGAAAAAA 
CCTTCCCTCG GAGACGATGA GGAGAGAGAA GAGGTTCCGA TACTTTTCCG AGATGTCACC 
TCTCTTTTGC TCATTCAGGT TCTAACAATG CCCCAGCCAT TGTGCAAAGA GCATTTTGTT 
TGCATTGTGA AAGTCCTCTA CACGTTGCTA TACACTCAAG CGATCACATC CCTCTCGGTA 
AAACTCCAGG CTGAAGATCG CAAGGTTTGG AGGGAATCTG GATCCAGCAA GAAGAGTTCA 
TATTCTTCTG GAAAACCTTG GGAAGCTTTG CTTGGGTTTG TGATAAGTGA ACTATCAAGA 
GGGAGCTTAT ACCAAGATGA AGAGACTGAC AGTATAAACA TGAGTGACTT CACAGCTTGG 
TACGAAGACG CCATTGAGCA TTATTTACAG CAGTTTTGCT TACCTTTCCT CAGGATTACA 
AGCTTTCTCC AGCATCACCT TTTCGGATTA GATTTGCCAA GTTGCCAGGA AGAAGATGAA 
TTTACTGTCC TTTCCAACTG TTTGGGACTT TTGCCATCTT TCAGTGAAGC ATCGCACCAG 
TTTGTCAGTG CCTCCTTACT TGACTGGCCA GTGTCCGCAT TTGATTTAAT ATCTCAGTGG 
TGTTCAGAAG TCAATTTGTT TTCAGACAAG CACCCAGACC AAGCTAACGT TTTGCTTATT 
CAAGATACCA AGTGGGACTT ACCACGCCTC CTTCAGTTGC CAGAAAACTA CAATACAATA 
TTTCAATATT ATCATAGAAA GTGCTGCAGT GTCTGTACAA AGGTTCCCAA GGATCCTGCA 
CTTTGCCTAG TTTGTGGCAC ATTTGTTTGC TTAAAGGGCC TCTGCTGCAA ACAACAGAGT 
TACTGTGAAT GTGTATTGCA CTCCCAAAAC TGTGGAGCCG GCACTGGAAT TTTCCTCTTG 
ATCAACGCTT CTGTCATCAT TATTATTCGA GGCCATCGCT TCTGCCTTTG GGGCTCTGTA 
TATCTCGATG CACATGGGGA GGAAGACAGA GACCTTAGGC GCGGAAAGCC CCTGTACATC 
TGTAAGGAAA GGTACAGCAT GCTGGAGCAG CAATGGGTCT CGCACACGTT TGATCACATC 
AATAAGAGAT GGGGGCCGCA TTACAATGGC TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ubr3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

サポート

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