tcof1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following tcof1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tcof1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa54354 XM_012958674.3
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa54355 XM_031898716.1
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa54356 XM_031898717.1
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa54357 XM_031898718.1
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa54358 XM_031898719.1
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa54359 XM_031898720.1
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa54360 XM_031898721.1
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa54361 XM_031898722.1
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa54362 XM_031898723.1
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa21469 NM_001142109.1
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OXa32615 XM_012958673.2
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OXa32616 XM_012958674.2
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OXa32617 XM_012958675.2
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OXa32618 XM_012958677.2
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OXa32619 XM_012958678.2
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OXa32620 XM_012958679.2
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OXa32621 XM_012958680.2
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OXa32622 XM_012958681.2
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OXa43053 XM_018091967.1
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OXa43054 XM_018091968.1
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OXa43055 XM_018091969.1
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OXa43056 XM_018091971.1
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OXa43057 XM_018091972.1
Latest version!
Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa54354
    Clone ID Related Accession (Same CDS sequence) XM_012958674.3
    Accession Version XM_012958674.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958674.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCGTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC CTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGC AGAAGACTGC GGCACCTCCC
    CAGGCATCCC AGAAAAAGGG CAAATCGCCT CTCTTGGCCA GTACAGGGAA TGCTGCAACA
    CCAGCCAAGA CACCAGTGGT TGCACAGCCC CTAACAGCTC AGAGCTCTGA GAGCAGCAAC
    TCGTCAGACA GTGAGGAGGA GCCTGATGCT AAGAAAATAA ATGCATCCTT GAACCCTACA
    ACATCAGCGA GCCAAGCTTT TGCCACTATG AAGCCAACTG CCAAACAGGA CTGTGACAGT
    AGTGACACAA GTGAAGAAGC CGAGGGCAAA TCCAGGAGTC TGGTGGGGCA ACCATCGGTT
    ACCATTATCT CGGAGACCAG TGATACCACT GATATTGATG AGGTAGTGTC TCCAACTCCT
    GCTCCAATCA AGGCAACCAA AGGAAAGGCT GTACCCATAT TGGCAAAGAC TGTCACACCA
    TCCAGCCTAA AAGCTACCAA AAGCAAAGCC AGCAGTGCAG CTAAAACTGC CAAAGCAGTG
    CAGCAAATAG CTGTAGCCAA CTCTGACAGC AGTGATTCTT TAGATAGCGA TGAGGATCCT
    GGAGTAAAGC AGACACCATC TGTACCATTA AAGAAAGTAA AGTCAGGGAA AGGAGCTAAA
    CTGACTGCCG TGTGTGTCAG TGGTGGAAAT CAGAAACTGC CCAGTTCGTT ACCTGTGCCC
    TCTTTACAAA AGCCCAGTGT ATCTGACGTT TCTGAGCCCA GCGACTCGTC CGATAGTGAC
    ATCGATACAG GAGCCCAGTC TCTTGTAGCA CCAGTACATG CGGTGCCCAC CCCACAGAAA
    CGGAAGAGGA ATCCAAAAGT CAAAGCTCAA GTGACGCAAC CTCCAGCTGC TTCAGTGAAA
    CACAAAAAAA AGTCTAAAGC AGAGCAACTG GCAGCATCTG CACAGCCAGC ACCAGAGCCA
    GTGATCGACT CTGAAGAGGA GACCATAATG GCTCTCCTTT CGGGTCAGAG CCCAAAGAAG
    GGGAAGCAGA AACTATCCAA ACTGGGACTG AAAGCAGCTC CTTCAGCCAC TCCCCTTCCT
    GTACAAAAGA ATGGCAGTGT GTCGGACTCA TCCGATGACA GCGATATCGG AACTGCAACA
    TTAAATATAG TTACACCTCC AGCGTTGGAG CTCAGGCCGC CGACCGCTGA CAAAGAAAAG
    AAAAGTGCAA AGAAGAGGAA GAGCTCCTCA GAAGATGGAC CCAAAAAGAA AGCTAAGAAA
    GCAAAGAAGG AAGGAAAGAA AGAAAAGAAG AAATCAAAAG CAGAAGGAGG AGAGGCTGGA
    GCCAGCACCC CTAAAGCAAA GTCCTCCAAA AAAGATAAAA AGAAGCTAGA GAAATCAGAG
    AAAAAGAAAT CAAGCAAAAA AAAAAAGGAA AAGAAAAAGA AGGATGGGGA AAAAAAGGAG
    GCAAAGAAGA CCAAGAAGGG TTCAGCAACT GGCGTCTCCC CTAAACCAAA GAAAAAGAAG
    AAAAGTAAAG CGGATAAAGT TGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814128.2
    CDS159..4565
    Translation

    Target ORF information:

    RefSeq Version XM_012958674.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958674.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCGTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC CTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGC AGAAGACTGC GGCACCTCCC
    CAGGCATCCC AGAAAAAGGG CAAATCGCCT CTCTTGGCCA GTACAGGGAA TGCTGCAACA
    CCAGCCAAGA CACCAGTGGT TGCACAGCCC CTAACAGCTC AGAGCTCTGA GAGCAGCAAC
    TCGTCAGACA GTGAGGAGGA GCCTGATGCT AAGAAAATAA ATGCATCCTT GAACCCTACA
    ACATCAGCGA GCCAAGCTTT TGCCACTATG AAGCCAACTG CCAAACAGGA CTGTGACAGT
    AGTGACACAA GTGAAGAAGC CGAGGGCAAA TCCAGGAGTC TGGTGGGGCA ACCATCGGTT
    ACCATTATCT CGGAGACCAG TGATACCACT GATATTGATG AGGTAGTGTC TCCAACTCCT
    GCTCCAATCA AGGCAACCAA AGGAAAGGCT GTACCCATAT TGGCAAAGAC TGTCACACCA
    TCCAGCCTAA AAGCTACCAA AAGCAAAGCC AGCAGTGCAG CTAAAACTGC CAAAGCAGTG
    CAGCAAATAG CTGTAGCCAA CTCTGACAGC AGTGATTCTT TAGATAGCGA TGAGGATCCT
    GGAGTAAAGC AGACACCATC TGTACCATTA AAGAAAGTAA AGTCAGGGAA AGGAGCTAAA
    CTGACTGCCG TGTGTGTCAG TGGTGGAAAT CAGAAACTGC CCAGTTCGTT ACCTGTGCCC
    TCTTTACAAA AGCCCAGTGT ATCTGACGTT TCTGAGCCCA GCGACTCGTC CGATAGTGAC
    ATCGATACAG GAGCCCAGTC TCTTGTAGCA CCAGTACATG CGGTGCCCAC CCCACAGAAA
    CGGAAGAGGA ATCCAAAAGT CAAAGCTCAA GTGACGCAAC CTCCAGCTGC TTCAGTGAAA
    CACAAAAAAA AGTCTAAAGC AGAGCAACTG GCAGCATCTG CACAGCCAGC ACCAGAGCCA
    GTGATCGACT CTGAAGAGGA GACCATAATG GCTCTCCTTT CGGGTCAGAG CCCAAAGAAG
    GGGAAGCAGA AACTATCCAA ACTGGGACTG AAAGCAGCTC CTTCAGCCAC TCCCCTTCCT
    GTACAAAAGA ATGGCAGTGT GTCGGACTCA TCCGATGACA GCGATATCGG AACTGCAACA
    TTAAATATAG TTACACCTCC AGCGTTGGAG CTCAGGCCGC CGACCGCTGA CAAAGAAAAG
    AAAAGTGCAA AGAAGAGGAA GAGCTCCTCA GAAGATGGAC CCAAAAAGAA AGCTAAGAAA
    GCAAAGAAGG AAGGAAAGAA AGAAAAGAAG AAATCAAAAG CAGAAGGAGG AGAGGCTGGA
    GCCAGCACCC CTAAAGCAAA GTCCTCCAAA AAAGATAAAA AGAAGCTAGA GAAATCAGAG
    AAAAAGAAAT CAAGCAAAAA AAAAAAGGAA AAGAAAAAGA AGGATGGGGA AAAAAAGGAG
    GCAAAGAAGA CCAAGAAGGG TTCAGCAACT GGCGTCTCCC CTAAACCAAA GAAAAAGAAG
    AAAAGTAAAG CGGATAAAGT TGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54355
    Clone ID Related Accession (Same CDS sequence) XM_031898716.1
    Accession Version XM_031898716.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4404bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCGTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC CTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGA AGACTGCGGC ACCTCCCCAG
    GCATCCCAGA AAAAGGGCAA ATCGCCTCTC TTGGCCAGTA CAGGGAATGC TGCAACACCA
    GCCAAGACAC CAGTGGTTGC ACAGCCCCTA ACAGCTCAGA GCTCTGAGAG CAGCAACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAATAAATG CATCCTTGAA CCCTACAACA
    TCAGCGAGCC AAGCTTTTGC CACTATGAAG CCAACTGCCA AACAGGACTG TGACAGTAGT
    GACACAAGTG AAGAAGCCGA GGGCAAATCC AGGAGTCTGG TGGGGCAACC ATCGGTTACC
    ATTATCTCGG AGACCAGTGA TACCACTGAT ATTGATGAGG TAGTGTCTCC AACTCCTGCT
    CCAATCAAGG CAACCAAAGG AAAGGCTGTA CCCATATTGG CAAAGACTGT CACACCATCC
    AGCCTAAAAG CTACCAAAAG CAAAGCCAGC AGTGCAGCTA AAACTGCCAA AGCAGTGCAG
    CAAATAGCTG TAGCCAACTC TGACAGCAGT GATTCTTTAG ATAGCGATGA GGATCCTGGA
    GTAAAGCAGA CACCATCTGT ACCATTAAAG AAAGTAAAGT CAGGGAAAGG AGCTAAACTG
    ACTGCCGTGT GTGTCAGTGG TGGAAATCAG AAACTGCCCA GTTCGTTACC TGTGCCCTCT
    TTACAAAAGC CCAGTGTATC TGACGTTTCT GAGCCCAGCG ACTCGTCCGA TAGTGACATC
    GATACAGGAG CCCAGTCTCT TGTAGCACCA GTACATGCGG TGCCCACCCC ACAGAAACGG
    AAGAGGAATC CAAAAGTCAA AGCTCAAGTG ACGCAACCTC CAGCTGCTTC AGTGAAACAC
    AAAAAAAAGT CTAAAGCAGA GCAACTGGCA GCATCTGCAC AGCCAGCACC AGAGCCAGTG
    ATCGACTCTG AAGAGGAGAC CATAATGGCT CTCCTTTCGG GTCAGAGCCC AAAGAAGGGG
    AAGCAGAAAC TATCCAAACT GGGACTGAAA GCAGCTCCTT CAGCCACTCC CCTTCCTGTA
    CAAAAGAATG GCAGTGTGTC GGACTCATCC GATGACAGCG ATATCGGAAC TGCAACATTA
    AATATAGTTA CACCTCCAGC GTTGGAGCTC AGGCCGCCGA CCGCTGACAA AGAAAAGAAA
    AGTGCAAAGA AGAGGAAGAG CTCCTCAGAA GATGGACCCA AAAAGAAAGC TAAGAAAGCA
    AAGAAGGAAG GAAAGAAAGA AAAGAAGAAA TCAAAAGCAG AAGGAGGAGA GGCTGGAGCC
    AGCACCCCTA AAGCAAAGTC CTCCAAAAAA GATAAAAAGA AGCTAGAGAA ATCAGAGAAA
    AAGAAATCAA GCAAAAAAAA AAAGGAAAAG AAAAAGAAGG ATGGGGAAAA AAAGGAGGCA
    AAGAAGACCA AGAAGGGTTC AGCAACTGGC GTCTCCCCTA AACCAAAGAA AAAGAAGAAA
    AGTAAAGCGG ATAAAGTTGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754576.1
    CDS159..4562
    Translation

    Target ORF information:

    RefSeq Version XM_031898716.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898716.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCGTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC CTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGA AGACTGCGGC ACCTCCCCAG
    GCATCCCAGA AAAAGGGCAA ATCGCCTCTC TTGGCCAGTA CAGGGAATGC TGCAACACCA
    GCCAAGACAC CAGTGGTTGC ACAGCCCCTA ACAGCTCAGA GCTCTGAGAG CAGCAACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAATAAATG CATCCTTGAA CCCTACAACA
    TCAGCGAGCC AAGCTTTTGC CACTATGAAG CCAACTGCCA AACAGGACTG TGACAGTAGT
    GACACAAGTG AAGAAGCCGA GGGCAAATCC AGGAGTCTGG TGGGGCAACC ATCGGTTACC
    ATTATCTCGG AGACCAGTGA TACCACTGAT ATTGATGAGG TAGTGTCTCC AACTCCTGCT
    CCAATCAAGG CAACCAAAGG AAAGGCTGTA CCCATATTGG CAAAGACTGT CACACCATCC
    AGCCTAAAAG CTACCAAAAG CAAAGCCAGC AGTGCAGCTA AAACTGCCAA AGCAGTGCAG
    CAAATAGCTG TAGCCAACTC TGACAGCAGT GATTCTTTAG ATAGCGATGA GGATCCTGGA
    GTAAAGCAGA CACCATCTGT ACCATTAAAG AAAGTAAAGT CAGGGAAAGG AGCTAAACTG
    ACTGCCGTGT GTGTCAGTGG TGGAAATCAG AAACTGCCCA GTTCGTTACC TGTGCCCTCT
    TTACAAAAGC CCAGTGTATC TGACGTTTCT GAGCCCAGCG ACTCGTCCGA TAGTGACATC
    GATACAGGAG CCCAGTCTCT TGTAGCACCA GTACATGCGG TGCCCACCCC ACAGAAACGG
    AAGAGGAATC CAAAAGTCAA AGCTCAAGTG ACGCAACCTC CAGCTGCTTC AGTGAAACAC
    AAAAAAAAGT CTAAAGCAGA GCAACTGGCA GCATCTGCAC AGCCAGCACC AGAGCCAGTG
    ATCGACTCTG AAGAGGAGAC CATAATGGCT CTCCTTTCGG GTCAGAGCCC AAAGAAGGGG
    AAGCAGAAAC TATCCAAACT GGGACTGAAA GCAGCTCCTT CAGCCACTCC CCTTCCTGTA
    CAAAAGAATG GCAGTGTGTC GGACTCATCC GATGACAGCG ATATCGGAAC TGCAACATTA
    AATATAGTTA CACCTCCAGC GTTGGAGCTC AGGCCGCCGA CCGCTGACAA AGAAAAGAAA
    AGTGCAAAGA AGAGGAAGAG CTCCTCAGAA GATGGACCCA AAAAGAAAGC TAAGAAAGCA
    AAGAAGGAAG GAAAGAAAGA AAAGAAGAAA TCAAAAGCAG AAGGAGGAGA GGCTGGAGCC
    AGCACCCCTA AAGCAAAGTC CTCCAAAAAA GATAAAAAGA AGCTAGAGAA ATCAGAGAAA
    AAGAAATCAA GCAAAAAAAA AAAGGAAAAG AAAAAGAAGG ATGGGGAAAA AAAGGAGGCA
    AAGAAGACCA AGAAGGGTTC AGCAACTGGC GTCTCCCCTA AACCAAAGAA AAAGAAGAAA
    AGTAAAGCGG ATAAAGTTGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54356
    Clone ID Related Accession (Same CDS sequence) XM_031898717.1
    Accession Version XM_031898717.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCGTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC CTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGC AGAAGACTGC GGCACCTCCC
    CAGGCATCCC AGAAAAAGGG CAAATCGCCT CTCTTGGCCA GTACAGGGAA TGCTGCAACA
    CCAGCCAAGA CACCAGTGGT TGCACAGCCC CTAACAGCTC AGAGCTCTGA GAGCAGCAAC
    TCGTCAGACA GTGAGGAGGA GCCTGATGCT AAGGTAGTGT CTCCAACTCC TGCTCCAATC
    AAGGCAACCA AAGGAAAGGC TGTACCCATA TTGGCAAAGA CTGTCACACC ATCCAGCCTA
    AAAGCTACCA AAAGCAAAGC CAGCAGTGCA GCTAAAACTG CCAAAGCAGT GCAGCAAATA
    GCTGTAGCCA ACTCTGACAG CAGTGATTCT TTAGATAGCG ATGAGGATCC TGGAGTAAAG
    CAGACACCAT CTGTACCATT AAAGAAAGTA AAGTCAGGGA AAGGAGCTAA ACTGACTGCC
    GTGTGTGTCA GTGGTGGAAA TCAGAAACTG CCCAGTTCGT TACCTGTGCC CTCTTTACAA
    AAGCCCAGTG TATCTGACGT TTCTGAGCCC AGCGACTCGT CCGATAGTGA CATCGATACA
    GGAGCCCAGT CTCTTGTAGC ACCAGTACAT GCGGTGCCCA CCCCACAGAA ACGGAAGAGG
    AATCCAAAAG TCAAAGCTCA AGTGACGCAA CCTCCAGCTG CTTCAGTGAA ACACAAAAAA
    AAGTCTAAAG CAGAGCAACT GGCAGCATCT GCACAGCCAG CACCAGAGCC AGTGATCGAC
    TCTGAAGAGG AGACCATAAT GGCTCTCCTT TCGGGTCAGA GCCCAAAGAA GGGGAAGCAG
    AAACTATCCA AACTGGGACT GAAAGCAGCT CCTTCAGCCA CTCCCCTTCC TGTACAAAAG
    AATGGCAGTG TGTCGGACTC ATCCGATGAC AGCGATATCG GAACTGCAAC ATTAAATATA
    GTTACACCTC CAGCGTTGGA GCTCAGGCCG CCGACCGCTG ACAAAGAAAA GAAAAGTGCA
    AAGAAGAGGA AGAGCTCCTC AGAAGATGGA CCCAAAAAGA AAGCTAAGAA AGCAAAGAAG
    GAAGGAAAGA AAGAAAAGAA GAAATCAAAA GCAGAAGGAG GAGAGGCTGG AGCCAGCACC
    CCTAAAGCAA AGTCCTCCAA AAAAGATAAA AAGAAGCTAG AGAAATCAGA GAAAAAGAAA
    TCAAGCAAAA AAAAAAAGGA AAAGAAAAAG AAGGATGGGG AAAAAAAGGA GGCAAAGAAG
    ACCAAGAAGG GTTCAGCAAC TGGCGTCTCC CCTAAACCAA AGAAAAAGAA GAAAAGTAAA
    GCGGATAAAG TTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754577.1
    CDS159..4376
    Translation

    Target ORF information:

    RefSeq Version XM_031898717.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898717.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCGTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC CTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGC AGAAGACTGC GGCACCTCCC
    CAGGCATCCC AGAAAAAGGG CAAATCGCCT CTCTTGGCCA GTACAGGGAA TGCTGCAACA
    CCAGCCAAGA CACCAGTGGT TGCACAGCCC CTAACAGCTC AGAGCTCTGA GAGCAGCAAC
    TCGTCAGACA GTGAGGAGGA GCCTGATGCT AAGGTAGTGT CTCCAACTCC TGCTCCAATC
    AAGGCAACCA AAGGAAAGGC TGTACCCATA TTGGCAAAGA CTGTCACACC ATCCAGCCTA
    AAAGCTACCA AAAGCAAAGC CAGCAGTGCA GCTAAAACTG CCAAAGCAGT GCAGCAAATA
    GCTGTAGCCA ACTCTGACAG CAGTGATTCT TTAGATAGCG ATGAGGATCC TGGAGTAAAG
    CAGACACCAT CTGTACCATT AAAGAAAGTA AAGTCAGGGA AAGGAGCTAA ACTGACTGCC
    GTGTGTGTCA GTGGTGGAAA TCAGAAACTG CCCAGTTCGT TACCTGTGCC CTCTTTACAA
    AAGCCCAGTG TATCTGACGT TTCTGAGCCC AGCGACTCGT CCGATAGTGA CATCGATACA
    GGAGCCCAGT CTCTTGTAGC ACCAGTACAT GCGGTGCCCA CCCCACAGAA ACGGAAGAGG
    AATCCAAAAG TCAAAGCTCA AGTGACGCAA CCTCCAGCTG CTTCAGTGAA ACACAAAAAA
    AAGTCTAAAG CAGAGCAACT GGCAGCATCT GCACAGCCAG CACCAGAGCC AGTGATCGAC
    TCTGAAGAGG AGACCATAAT GGCTCTCCTT TCGGGTCAGA GCCCAAAGAA GGGGAAGCAG
    AAACTATCCA AACTGGGACT GAAAGCAGCT CCTTCAGCCA CTCCCCTTCC TGTACAAAAG
    AATGGCAGTG TGTCGGACTC ATCCGATGAC AGCGATATCG GAACTGCAAC ATTAAATATA
    GTTACACCTC CAGCGTTGGA GCTCAGGCCG CCGACCGCTG ACAAAGAAAA GAAAAGTGCA
    AAGAAGAGGA AGAGCTCCTC AGAAGATGGA CCCAAAAAGA AAGCTAAGAA AGCAAAGAAG
    GAAGGAAAGA AAGAAAAGAA GAAATCAAAA GCAGAAGGAG GAGAGGCTGG AGCCAGCACC
    CCTAAAGCAA AGTCCTCCAA AAAAGATAAA AAGAAGCTAG AGAAATCAGA GAAAAAGAAA
    TCAAGCAAAA AAAAAAAGGA AAAGAAAAAG AAGGATGGGG AAAAAAAGGA GGCAAAGAAG
    ACCAAGAAGG GTTCAGCAAC TGGCGTCTCC CCTAAACCAA AGAAAAAGAA GAAAAGTAAA
    GCGGATAAAG TTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54357
    Clone ID Related Accession (Same CDS sequence) XM_031898718.1
    Accession Version XM_031898718.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA CACATACAGT TTTGGCTCAT GTTCATGCAC CACCAAGGAC ACCTCTGCTA
    TCTCCTTCCA AAACTGCAAG GGCTGCTGTG CCAGCAAAAG CTGCTGTTGT CACTAAACCA
    GTAGATATCG ATGAGTCGGA GAGCAGTGAT TCATCGGACA GTGAAATCAT AGTTACCCAG
    GCATCCCAGA AAAAGCCCAG TGATCCTACA GCCGCTAAGC CACTAGTGAC AAAAACACCC
    AACTTGGCCC AACCTGGAAA GGTGGCAACG CCAACAAAGA CACCAGTGAG TGCAAAGCCC
    CTAACAGCTC AGAGCTCTGA GAGCAGCGAC TCGTCAGACA GTGAGGAGGA GCTTAATGCT
    AAGAATACAG CAGCACCTCT CCAGGCATCC CAGAAAAAGA CTATTGTTCC TCCCACATCA
    ACAAGGCCAC CAGGGACAAA AACACCCCTC TTGGCCAAAC CCGGAACATT GACAACACCA
    GCCAAGGCAT CTGTGGTTGC TCAGAGCTCT GAGAGCAGCG ACTCGTCAGA CAGTGAGTTG
    GAGCCTGATG CTAAGAAAAC AGCAGCGCCT CTCCAAGGAT CCCAAAAAAA TATACCTGCC
    ACACCTTCTA CAGCCACTAG GCCACCAGGG ACAAAAACAC CCCTCTTGGC CAAACCTGGG
    AAACTGGCAA CACCAACCAA GGCATCTGTG GTTGCTAAGC CACTGGCAGC TCAAAGCTCT
    GAGAGCAGTG ACTCGTCCGA CAGTGAGGAG GAGCCTGATA CTAAGAAGAC AGCCGTGCCT
    CTCCAGGGAT CTCAGAAAAA AACACCTGTC ACACCTTCCA CAGCCGCTAG ACCACCAGGG
    ACAAAAACAC CCCTCTTGGC CAAACCCGGA ACATTGGCAA CACCAGCCAA AGCATCTGTA
    GTTCCTCAGA GCTCTGAGAG CAGTGACTCG TCAGACAGTG AGTTGGAGCC TGATGCTAAG
    AAAACAGCAG CGCCTCTCCA AGGATCCCAA AAAAATATAC CTGCCACACC TTCTACAGCC
    ACTAGGCCAC CAGGGACAAA AACACCCCTC TTGGCCAAAC CTGGGAAACT GGCAACACCA
    ACCAAGGCAT CTGTGGTTGC TCAGCCACTG GCAGCTCAAA GCTCTGAGAG CAGCGACTCG
    TCCGACAGTG AGGAGGAGCC TGATACTAAG AAGACAGCCG TGCCTCTCCA GGGATCTCAG
    AAAAAAACAC CTGTCACACC TTCCACAGCC GCTAGACCAC CAGGGACAAA AACACCCCTC
    TTGGCCAAAC CCGGAACATT GGCAACACCA GCCAAGGCAT CTGTAGTTGC TCAGAGCTCT
    GAGAGCAGTG ACTCGTCAGA CAGTGAGTTG GAGCCTGATG CTAAGAAAAC AGCAGCGTCT
    CTCCAAGGAT CCCAGAAAAA TACACCAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGGCCAAAC CTGGAAATTT GGCAACACCA GCCAAGGCAT CTATGGTTGC TCAGCCACCG
    GCAGCTCAGA GCTCTGAGAG CAGCGACTTG TCAGACAGTG AGGAGGAACC TAATGCTAAG
    AAAACAGCAG CGCCTCTCCA GGGATCCCAG AAAAAGACAC CTGCCACAGC CGCTAGGCCA
    CCAGAGACAA AAGCACCCCT CTTGGCCAAA CCTGGAAAAC TGGCAACACC CACCAAGGCA
    TCTGTGGTTG CACAGCCACT GGCAGCTCAG AGCTCTGAGA GCAGCGACTC GTCAGACAGT
    GAGGAGGAGC CTGATGCTAA GAAAACAGCA GCGCCTCTCC AGGGATCCCA GAAAAAGACA
    CCTGCCACAC CTTCCACAGC CACTAGGCCA CCAGGGACAA AAACACCCCT CTTGACTAAA
    CCTGGAAAAC TTGCAACACC AACCAAGACA CCAGTGATTT CTTTGCCGTT AGCAGAAAGT
    AGTGACTCGT CAGAAAGTGA AGACGAGCCA GCAGTCAAGA ATGCACAACT ATCTGTCGTG
    GCGTCCAAGA ACAATAGCAA AACGACTCTC TTGGCCAAGA CAGGGAAGTT GGCTGCTCCA
    GCCAAGACGC CAGCGGTTAC ACAGCCGCCA GTAGCCGAGA GCTCTGAGAG CAGCAACTCA
    TCTGATAGTG AGGAGGAGCT TAACAATAAG CAGAAGACTG CGGCACCTCC CCAGGCATCC
    CAGAAAAAGG GCAAATCGCC TCTCTTGGCC AGTACAGGGA ATGCTGCAAC ACCAGCCAAG
    ACACCAGTGG TTGCACAGCC CCTAACAGCT CAGAGCTCTG AGAGCAGCAA CTCGTCAGAC
    AGTGAGGAGG AGCCTGATGC TAAGAAAATA AATGCATCCT TGAACCCTAC AACATCAGCG
    AGCCAAGCTT TTGCCACTAT GAAGCCAACT GCCAAACAGG ACTGTGACAG TAGTGACACA
    AGTGAAGAAG CCGAGGGCAA ATCCAGGAGT CTGGTGGGGC AACCATCGGT TACCATTATC
    TCGGAGACCA GTGATACCAC TGATATTGAT GAGGTAGTGT CTCCAACTCC TGCTCCAATC
    AAGGCAACCA AAGGAAAGGC TGTACCCATA TTGGCAAAGA CTGTCACACC ATCCAGCCTA
    AAAGCTACCA AAAGCAAAGC CAGCAGTGCA GCTAAAACTG CCAAAGCAGT GCAGCAAATA
    GCTGTAGCCA ACTCTGACAG CAGTGATTCT TTAGATAGCG ATGAGGATCC TGGAGTAAAG
    CAGACACCAT CTGTACCATT AAAGAAAGTA AAGTCAGGGA AAGGAGCTAA ACTGACTGCC
    GTGTGTGTCA GTGGTGGAAA TCAGAAACTG CCCAGTTCGT TACCTGTGCC CTCTTTACAA
    AAGCCCAGTG TATCTGACGT TTCTGAGCCC AGCGACTCGT CCGATAGTGA CATCGATACA
    GGAGCCCAGT CTCTTGTAGC ACCAGTACAT GCGGTGCCCA CCCCACAGAA ACGGAAGAGG
    AATCCAAAAG TCAAAGCTCA AGTGACGCAA CCTCCAGCTG CTTCAGTGAA ACACAAAAAA
    AAGTCTAAAG CAGAGCAACT GGCAGCATCT GCACAGCCAG CACCAGAGCC AGTGATCGAC
    TCTGAAGAGG AGACCATAAT GGCTCTCCTT TCGGGTCAGA GCCCAAAGAA GGGGAAGCAG
    AAACTATCCA AACTGGGACT GAAAGCAGCT CCTTCAGCCA CTCCCCTTCC TGTACAAAAG
    AATGGCAGTG TGTCGGACTC ATCCGATGAC AGCGATATCG GAACTGCAAC ATTAAATATA
    GTTACACCTC CAGCGTTGGA GCTCAGGCCG CCGACCGCTG ACAAAGAAAA GAAAAGTGCA
    AAGAAGAGGA AGAGCTCCTC AGAAGATGGA CCCAAAAAGA AAGCTAAGAA AGCAAAGAAG
    GAAGGAAAGA AAGAAAAGAA GAAATCAAAA GCAGAAGGAG GAGAGGCTGG AGCCAGCACC
    CCTAAAGCAA AGTCCTCCAA AAAAGATAAA AAGAAGCTAG AGAAATCAGA GAAAAAGAAA
    TCAAGCAAAA AAAAAAAGGA AAAGAAAAAG AAGGATGGGG AAAAAAAGGA GGCAAAGAAG
    ACCAAGAAGG GTTCAGCAAC TGGCGTCTCC CCTAAACCAA AGAAAAAGAA GAAAAGTAAA
    GCGGATAAAG TTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754578.1
    CDS159..4376
    Translation

    Target ORF information:

    RefSeq Version XM_031898718.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898718.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA CACATACAGT TTTGGCTCAT GTTCATGCAC CACCAAGGAC ACCTCTGCTA
    TCTCCTTCCA AAACTGCAAG GGCTGCTGTG CCAGCAAAAG CTGCTGTTGT CACTAAACCA
    GTAGATATCG ATGAGTCGGA GAGCAGTGAT TCATCGGACA GTGAAATCAT AGTTACCCAG
    GCATCCCAGA AAAAGCCCAG TGATCCTACA GCCGCTAAGC CACTAGTGAC AAAAACACCC
    AACTTGGCCC AACCTGGAAA GGTGGCAACG CCAACAAAGA CACCAGTGAG TGCAAAGCCC
    CTAACAGCTC AGAGCTCTGA GAGCAGCGAC TCGTCAGACA GTGAGGAGGA GCTTAATGCT
    AAGAATACAG CAGCACCTCT CCAGGCATCC CAGAAAAAGA CTATTGTTCC TCCCACATCA
    ACAAGGCCAC CAGGGACAAA AACACCCCTC TTGGCCAAAC CCGGAACATT GACAACACCA
    GCCAAGGCAT CTGTGGTTGC TCAGAGCTCT GAGAGCAGCG ACTCGTCAGA CAGTGAGTTG
    GAGCCTGATG CTAAGAAAAC AGCAGCGCCT CTCCAAGGAT CCCAAAAAAA TATACCTGCC
    ACACCTTCTA CAGCCACTAG GCCACCAGGG ACAAAAACAC CCCTCTTGGC CAAACCTGGG
    AAACTGGCAA CACCAACCAA GGCATCTGTG GTTGCTAAGC CACTGGCAGC TCAAAGCTCT
    GAGAGCAGTG ACTCGTCCGA CAGTGAGGAG GAGCCTGATA CTAAGAAGAC AGCCGTGCCT
    CTCCAGGGAT CTCAGAAAAA AACACCTGTC ACACCTTCCA CAGCCGCTAG ACCACCAGGG
    ACAAAAACAC CCCTCTTGGC CAAACCCGGA ACATTGGCAA CACCAGCCAA AGCATCTGTA
    GTTCCTCAGA GCTCTGAGAG CAGTGACTCG TCAGACAGTG AGTTGGAGCC TGATGCTAAG
    AAAACAGCAG CGCCTCTCCA AGGATCCCAA AAAAATATAC CTGCCACACC TTCTACAGCC
    ACTAGGCCAC CAGGGACAAA AACACCCCTC TTGGCCAAAC CTGGGAAACT GGCAACACCA
    ACCAAGGCAT CTGTGGTTGC TCAGCCACTG GCAGCTCAAA GCTCTGAGAG CAGCGACTCG
    TCCGACAGTG AGGAGGAGCC TGATACTAAG AAGACAGCCG TGCCTCTCCA GGGATCTCAG
    AAAAAAACAC CTGTCACACC TTCCACAGCC GCTAGACCAC CAGGGACAAA AACACCCCTC
    TTGGCCAAAC CCGGAACATT GGCAACACCA GCCAAGGCAT CTGTAGTTGC TCAGAGCTCT
    GAGAGCAGTG ACTCGTCAGA CAGTGAGTTG GAGCCTGATG CTAAGAAAAC AGCAGCGTCT
    CTCCAAGGAT CCCAGAAAAA TACACCAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGGCCAAAC CTGGAAATTT GGCAACACCA GCCAAGGCAT CTATGGTTGC TCAGCCACCG
    GCAGCTCAGA GCTCTGAGAG CAGCGACTTG TCAGACAGTG AGGAGGAACC TAATGCTAAG
    AAAACAGCAG CGCCTCTCCA GGGATCCCAG AAAAAGACAC CTGCCACAGC CGCTAGGCCA
    CCAGAGACAA AAGCACCCCT CTTGGCCAAA CCTGGAAAAC TGGCAACACC CACCAAGGCA
    TCTGTGGTTG CACAGCCACT GGCAGCTCAG AGCTCTGAGA GCAGCGACTC GTCAGACAGT
    GAGGAGGAGC CTGATGCTAA GAAAACAGCA GCGCCTCTCC AGGGATCCCA GAAAAAGACA
    CCTGCCACAC CTTCCACAGC CACTAGGCCA CCAGGGACAA AAACACCCCT CTTGACTAAA
    CCTGGAAAAC TTGCAACACC AACCAAGACA CCAGTGATTT CTTTGCCGTT AGCAGAAAGT
    AGTGACTCGT CAGAAAGTGA AGACGAGCCA GCAGTCAAGA ATGCACAACT ATCTGTCGTG
    GCGTCCAAGA ACAATAGCAA AACGACTCTC TTGGCCAAGA CAGGGAAGTT GGCTGCTCCA
    GCCAAGACGC CAGCGGTTAC ACAGCCGCCA GTAGCCGAGA GCTCTGAGAG CAGCAACTCA
    TCTGATAGTG AGGAGGAGCT TAACAATAAG CAGAAGACTG CGGCACCTCC CCAGGCATCC
    CAGAAAAAGG GCAAATCGCC TCTCTTGGCC AGTACAGGGA ATGCTGCAAC ACCAGCCAAG
    ACACCAGTGG TTGCACAGCC CCTAACAGCT CAGAGCTCTG AGAGCAGCAA CTCGTCAGAC
    AGTGAGGAGG AGCCTGATGC TAAGAAAATA AATGCATCCT TGAACCCTAC AACATCAGCG
    AGCCAAGCTT TTGCCACTAT GAAGCCAACT GCCAAACAGG ACTGTGACAG TAGTGACACA
    AGTGAAGAAG CCGAGGGCAA ATCCAGGAGT CTGGTGGGGC AACCATCGGT TACCATTATC
    TCGGAGACCA GTGATACCAC TGATATTGAT GAGGTAGTGT CTCCAACTCC TGCTCCAATC
    AAGGCAACCA AAGGAAAGGC TGTACCCATA TTGGCAAAGA CTGTCACACC ATCCAGCCTA
    AAAGCTACCA AAAGCAAAGC CAGCAGTGCA GCTAAAACTG CCAAAGCAGT GCAGCAAATA
    GCTGTAGCCA ACTCTGACAG CAGTGATTCT TTAGATAGCG ATGAGGATCC TGGAGTAAAG
    CAGACACCAT CTGTACCATT AAAGAAAGTA AAGTCAGGGA AAGGAGCTAA ACTGACTGCC
    GTGTGTGTCA GTGGTGGAAA TCAGAAACTG CCCAGTTCGT TACCTGTGCC CTCTTTACAA
    AAGCCCAGTG TATCTGACGT TTCTGAGCCC AGCGACTCGT CCGATAGTGA CATCGATACA
    GGAGCCCAGT CTCTTGTAGC ACCAGTACAT GCGGTGCCCA CCCCACAGAA ACGGAAGAGG
    AATCCAAAAG TCAAAGCTCA AGTGACGCAA CCTCCAGCTG CTTCAGTGAA ACACAAAAAA
    AAGTCTAAAG CAGAGCAACT GGCAGCATCT GCACAGCCAG CACCAGAGCC AGTGATCGAC
    TCTGAAGAGG AGACCATAAT GGCTCTCCTT TCGGGTCAGA GCCCAAAGAA GGGGAAGCAG
    AAACTATCCA AACTGGGACT GAAAGCAGCT CCTTCAGCCA CTCCCCTTCC TGTACAAAAG
    AATGGCAGTG TGTCGGACTC ATCCGATGAC AGCGATATCG GAACTGCAAC ATTAAATATA
    GTTACACCTC CAGCGTTGGA GCTCAGGCCG CCGACCGCTG ACAAAGAAAA GAAAAGTGCA
    AAGAAGAGGA AGAGCTCCTC AGAAGATGGA CCCAAAAAGA AAGCTAAGAA AGCAAAGAAG
    GAAGGAAAGA AAGAAAAGAA GAAATCAAAA GCAGAAGGAG GAGAGGCTGG AGCCAGCACC
    CCTAAAGCAA AGTCCTCCAA AAAAGATAAA AAGAAGCTAG AGAAATCAGA GAAAAAGAAA
    TCAAGCAAAA AAAAAAAGGA AAAGAAAAAG AAGGATGGGG AAAAAAAGGA GGCAAAGAAG
    ACCAAGAAGG GTTCAGCAAC TGGCGTCTCC CCTAAACCAA AGAAAAAGAA GAAAAGTAAA
    GCGGATAAAG TTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54358
    Clone ID Related Accession (Same CDS sequence) XM_031898719.1
    Accession Version XM_031898719.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCGTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC CTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGA AGACTGCGGC ACCTCCCCAG
    GCATCCCAGA AAAAGGGCAA ATCGCCTCTC TTGGCCAGTA CAGGGAATGC TGCAACACCA
    GCCAAGACAC CAGTGGTTGC ACAGCCCCTA ACAGCTCAGA GCTCTGAGAG CAGCAACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG GTAGTGTCTC CAACTCCTGC TCCAATCAAG
    GCAACCAAAG GAAAGGCTGT ACCCATATTG GCAAAGACTG TCACACCATC CAGCCTAAAA
    GCTACCAAAA GCAAAGCCAG CAGTGCAGCT AAAACTGCCA AAGCAGTGCA GCAAATAGCT
    GTAGCCAACT CTGACAGCAG TGATTCTTTA GATAGCGATG AGGATCCTGG AGTAAAGCAG
    ACACCATCTG TACCATTAAA GAAAGTAAAG TCAGGGAAAG GAGCTAAACT GACTGCCGTG
    TGTGTCAGTG GTGGAAATCA GAAACTGCCC AGTTCGTTAC CTGTGCCCTC TTTACAAAAG
    CCCAGTGTAT CTGACGTTTC TGAGCCCAGC GACTCGTCCG ATAGTGACAT CGATACAGGA
    GCCCAGTCTC TTGTAGCACC AGTACATGCG GTGCCCACCC CACAGAAACG GAAGAGGAAT
    CCAAAAGTCA AAGCTCAAGT GACGCAACCT CCAGCTGCTT CAGTGAAACA CAAAAAAAAG
    TCTAAAGCAG AGCAACTGGC AGCATCTGCA CAGCCAGCAC CAGAGCCAGT GATCGACTCT
    GAAGAGGAGA CCATAATGGC TCTCCTTTCG GGTCAGAGCC CAAAGAAGGG GAAGCAGAAA
    CTATCCAAAC TGGGACTGAA AGCAGCTCCT TCAGCCACTC CCCTTCCTGT ACAAAAGAAT
    GGCAGTGTGT CGGACTCATC CGATGACAGC GATATCGGAA CTGCAACATT AAATATAGTT
    ACACCTCCAG CGTTGGAGCT CAGGCCGCCG ACCGCTGACA AAGAAAAGAA AAGTGCAAAG
    AAGAGGAAGA GCTCCTCAGA AGATGGACCC AAAAAGAAAG CTAAGAAAGC AAAGAAGGAA
    GGAAAGAAAG AAAAGAAGAA ATCAAAAGCA GAAGGAGGAG AGGCTGGAGC CAGCACCCCT
    AAAGCAAAGT CCTCCAAAAA AGATAAAAAG AAGCTAGAGA AATCAGAGAA AAAGAAATCA
    AGCAAAAAAA AAAAGGAAAA GAAAAAGAAG GATGGGGAAA AAAAGGAGGC AAAGAAGACC
    AAGAAGGGTT CAGCAACTGG CGTCTCCCCT AAACCAAAGA AAAAGAAGAA AAGTAAAGCG
    GATAAAGTTG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754579.1
    CDS159..4373
    Translation

    Target ORF information:

    RefSeq Version XM_031898719.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898719.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCGTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC CTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGA AGACTGCGGC ACCTCCCCAG
    GCATCCCAGA AAAAGGGCAA ATCGCCTCTC TTGGCCAGTA CAGGGAATGC TGCAACACCA
    GCCAAGACAC CAGTGGTTGC ACAGCCCCTA ACAGCTCAGA GCTCTGAGAG CAGCAACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG GTAGTGTCTC CAACTCCTGC TCCAATCAAG
    GCAACCAAAG GAAAGGCTGT ACCCATATTG GCAAAGACTG TCACACCATC CAGCCTAAAA
    GCTACCAAAA GCAAAGCCAG CAGTGCAGCT AAAACTGCCA AAGCAGTGCA GCAAATAGCT
    GTAGCCAACT CTGACAGCAG TGATTCTTTA GATAGCGATG AGGATCCTGG AGTAAAGCAG
    ACACCATCTG TACCATTAAA GAAAGTAAAG TCAGGGAAAG GAGCTAAACT GACTGCCGTG
    TGTGTCAGTG GTGGAAATCA GAAACTGCCC AGTTCGTTAC CTGTGCCCTC TTTACAAAAG
    CCCAGTGTAT CTGACGTTTC TGAGCCCAGC GACTCGTCCG ATAGTGACAT CGATACAGGA
    GCCCAGTCTC TTGTAGCACC AGTACATGCG GTGCCCACCC CACAGAAACG GAAGAGGAAT
    CCAAAAGTCA AAGCTCAAGT GACGCAACCT CCAGCTGCTT CAGTGAAACA CAAAAAAAAG
    TCTAAAGCAG AGCAACTGGC AGCATCTGCA CAGCCAGCAC CAGAGCCAGT GATCGACTCT
    GAAGAGGAGA CCATAATGGC TCTCCTTTCG GGTCAGAGCC CAAAGAAGGG GAAGCAGAAA
    CTATCCAAAC TGGGACTGAA AGCAGCTCCT TCAGCCACTC CCCTTCCTGT ACAAAAGAAT
    GGCAGTGTGT CGGACTCATC CGATGACAGC GATATCGGAA CTGCAACATT AAATATAGTT
    ACACCTCCAG CGTTGGAGCT CAGGCCGCCG ACCGCTGACA AAGAAAAGAA AAGTGCAAAG
    AAGAGGAAGA GCTCCTCAGA AGATGGACCC AAAAAGAAAG CTAAGAAAGC AAAGAAGGAA
    GGAAAGAAAG AAAAGAAGAA ATCAAAAGCA GAAGGAGGAG AGGCTGGAGC CAGCACCCCT
    AAAGCAAAGT CCTCCAAAAA AGATAAAAAG AAGCTAGAGA AATCAGAGAA AAAGAAATCA
    AGCAAAAAAA AAAAGGAAAA GAAAAAGAAG GATGGGGAAA AAAAGGAGGC AAAGAAGACC
    AAGAAGGGTT CAGCAACTGG CGTCTCCCCT AAACCAAAGA AAAAGAAGAA AAGTAAAGCG
    GATAAAGTTG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54359
    Clone ID Related Accession (Same CDS sequence) XM_031898720.1
    Accession Version XM_031898720.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCGCCTC TCCAGGGATC CCAGAAAAAG ACACCTGCCA CAGCCGCTAG GCCACCAGAG
    ACAAAAGCAC CCCTCTTGGC CAAACCTGGA AAACTGGCAA CACCCACCAA GGCATCTGTG
    GTTGCACAGC CACTGGCAGC TCAGAGCTCT GAGAGCAGCG ACTCGTCAGA CAGTGAGGAG
    GAGCCTGATG CTAAGAAAAC AGCAGCGCCT CTCCAGGGAT CCCAGAAAAA GACACCTGCC
    ACACCTTCCA CAGCCACTAG GCCACCAGGG ACAAAAACAC CCCTCTTGAC TAAACCTGGA
    AAACTTGCAA CACCAACCAA GACACCAGTG ATTTCTTTGC CGTTAGCAGA AAGTAGTGAC
    TCGTCAGAAA GTGAAGACGA GCCAGCAGTC AAGAATGCAC AACTATCTGT CGTGGCGTCC
    AAGAACAATA GCAAAACGAC TCTCTTGGCC AAGACAGGGA AGTTGGCTGC TCCAGCCAAG
    ACGCCAGCGG TTACACAGCC GCCAGTAGCC GAGAGCTCTG AGAGCAGCAA CTCATCTGAT
    AGTGAGGAGG AGCTTAACAA TAAGCAGAAG ACTGCGGCAC CTCCCCAGGC ATCCCAGAAA
    AAGGGCAAAT CGCCTCTCTT GGCCAGTACA GGGAATGCTG CAACACCAGC CAAGACACCA
    GTGGTTGCAC AGCCCCTAAC AGCTCAGAGC TCTGAGAGCA GCAACTCGTC AGACAGTGAG
    GAGGAGCCTG ATGCTAAGAA AATAAATGCA TCCTTGAACC CTACAACATC AGCGAGCCAA
    GCTTTTGCCA CTATGAAGCC AACTGCCAAA CAGGACTGTG ACAGTAGTGA CACAAGTGAA
    GAAGCCGAGG GCAAATCCAG GAGTCTGGTG GGGCAACCAT CGGTTACCAT TATCTCGGAG
    ACCAGTGATA CCACTGATAT TGATGAGGTA GTGTCTCCAA CTCCTGCTCC AATCAAGGCA
    ACCAAAGGAA AGGCTGTACC CATATTGGCA AAGACTGTCA CACCATCCAG CCTAAAAGCT
    ACCAAAAGCA AAGCCAGCAG TGCAGCTAAA ACTGCCAAAG CAGTGCAGCA AATAGCTGTA
    GCCAACTCTG ACAGCAGTGA TTCTTTAGAT AGCGATGAGG ATCCTGGAGT AAAGCAGACA
    CCATCTGTAC CATTAAAGAA AGTAAAGTCA GGGAAAGGAG CTAAACTGAC TGCCGTGTGT
    GTCAGTGGTG GAAATCAGAA ACTGCCCAGT TCGTTACCTG TGCCCTCTTT ACAAAAGCCC
    AGTGTATCTG ACGTTTCTGA GCCCAGCGAC TCGTCCGATA GTGACATCGA TACAGGAGCC
    CAGTCTCTTG TAGCACCAGT ACATGCGGTG CCCACCCCAC AGAAACGGAA GAGGAATCCA
    AAAGTCAAAG CTCAAGTGAC GCAACCTCCA GCTGCTTCAG TGAAACACAA AAAAAAGTCT
    AAAGCAGAGC AACTGGCAGC ATCTGCACAG CCAGCACCAG AGCCAGTGAT CGACTCTGAA
    GAGGAGACCA TAATGGCTCT CCTTTCGGGT CAGAGCCCAA AGAAGGGGAA GCAGAAACTA
    TCCAAACTGG GACTGAAAGC AGCTCCTTCA GCCACTCCCC TTCCTGTACA AAAGAATGGC
    AGTGTGTCGG ACTCATCCGA TGACAGCGAT ATCGGAACTG CAACATTAAA TATAGTTACA
    CCTCCAGCGT TGGAGCTCAG GCCGCCGACC GCTGACAAAG AAAAGAAAAG TGCAAAGAAG
    AGGAAGAGCT CCTCAGAAGA TGGACCCAAA AAGAAAGCTA AGAAAGCAAA GAAGGAAGGA
    AAGAAAGAAA AGAAGAAATC AAAAGCAGAA GGAGGAGAGG CTGGAGCCAG CACCCCTAAA
    GCAAAGTCCT CCAAAAAAGA TAAAAAGAAG CTAGAGAAAT CAGAGAAAAA GAAATCAAGC
    AAAAAAAAAA AGGAAAAGAA AAAGAAGGAT GGGGAAAAAA AGGAGGCAAA GAAGACCAAG
    AAGGGTTCAG CAACTGGCGT CTCCCCTAAA CCAAAGAAAA AGAAGAAAAG TAAAGCGGAT
    AAAGTTGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754580.1
    CDS159..4370
    Translation

    Target ORF information:

    RefSeq Version XM_031898720.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898720.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCGCCTC TCCAGGGATC CCAGAAAAAG ACACCTGCCA CAGCCGCTAG GCCACCAGAG
    ACAAAAGCAC CCCTCTTGGC CAAACCTGGA AAACTGGCAA CACCCACCAA GGCATCTGTG
    GTTGCACAGC CACTGGCAGC TCAGAGCTCT GAGAGCAGCG ACTCGTCAGA CAGTGAGGAG
    GAGCCTGATG CTAAGAAAAC AGCAGCGCCT CTCCAGGGAT CCCAGAAAAA GACACCTGCC
    ACACCTTCCA CAGCCACTAG GCCACCAGGG ACAAAAACAC CCCTCTTGAC TAAACCTGGA
    AAACTTGCAA CACCAACCAA GACACCAGTG ATTTCTTTGC CGTTAGCAGA AAGTAGTGAC
    TCGTCAGAAA GTGAAGACGA GCCAGCAGTC AAGAATGCAC AACTATCTGT CGTGGCGTCC
    AAGAACAATA GCAAAACGAC TCTCTTGGCC AAGACAGGGA AGTTGGCTGC TCCAGCCAAG
    ACGCCAGCGG TTACACAGCC GCCAGTAGCC GAGAGCTCTG AGAGCAGCAA CTCATCTGAT
    AGTGAGGAGG AGCTTAACAA TAAGCAGAAG ACTGCGGCAC CTCCCCAGGC ATCCCAGAAA
    AAGGGCAAAT CGCCTCTCTT GGCCAGTACA GGGAATGCTG CAACACCAGC CAAGACACCA
    GTGGTTGCAC AGCCCCTAAC AGCTCAGAGC TCTGAGAGCA GCAACTCGTC AGACAGTGAG
    GAGGAGCCTG ATGCTAAGAA AATAAATGCA TCCTTGAACC CTACAACATC AGCGAGCCAA
    GCTTTTGCCA CTATGAAGCC AACTGCCAAA CAGGACTGTG ACAGTAGTGA CACAAGTGAA
    GAAGCCGAGG GCAAATCCAG GAGTCTGGTG GGGCAACCAT CGGTTACCAT TATCTCGGAG
    ACCAGTGATA CCACTGATAT TGATGAGGTA GTGTCTCCAA CTCCTGCTCC AATCAAGGCA
    ACCAAAGGAA AGGCTGTACC CATATTGGCA AAGACTGTCA CACCATCCAG CCTAAAAGCT
    ACCAAAAGCA AAGCCAGCAG TGCAGCTAAA ACTGCCAAAG CAGTGCAGCA AATAGCTGTA
    GCCAACTCTG ACAGCAGTGA TTCTTTAGAT AGCGATGAGG ATCCTGGAGT AAAGCAGACA
    CCATCTGTAC CATTAAAGAA AGTAAAGTCA GGGAAAGGAG CTAAACTGAC TGCCGTGTGT
    GTCAGTGGTG GAAATCAGAA ACTGCCCAGT TCGTTACCTG TGCCCTCTTT ACAAAAGCCC
    AGTGTATCTG ACGTTTCTGA GCCCAGCGAC TCGTCCGATA GTGACATCGA TACAGGAGCC
    CAGTCTCTTG TAGCACCAGT ACATGCGGTG CCCACCCCAC AGAAACGGAA GAGGAATCCA
    AAAGTCAAAG CTCAAGTGAC GCAACCTCCA GCTGCTTCAG TGAAACACAA AAAAAAGTCT
    AAAGCAGAGC AACTGGCAGC ATCTGCACAG CCAGCACCAG AGCCAGTGAT CGACTCTGAA
    GAGGAGACCA TAATGGCTCT CCTTTCGGGT CAGAGCCCAA AGAAGGGGAA GCAGAAACTA
    TCCAAACTGG GACTGAAAGC AGCTCCTTCA GCCACTCCCC TTCCTGTACA AAAGAATGGC
    AGTGTGTCGG ACTCATCCGA TGACAGCGAT ATCGGAACTG CAACATTAAA TATAGTTACA
    CCTCCAGCGT TGGAGCTCAG GCCGCCGACC GCTGACAAAG AAAAGAAAAG TGCAAAGAAG
    AGGAAGAGCT CCTCAGAAGA TGGACCCAAA AAGAAAGCTA AGAAAGCAAA GAAGGAAGGA
    AAGAAAGAAA AGAAGAAATC AAAAGCAGAA GGAGGAGAGG CTGGAGCCAG CACCCCTAAA
    GCAAAGTCCT CCAAAAAAGA TAAAAAGAAG CTAGAGAAAT CAGAGAAAAA GAAATCAAGC
    AAAAAAAAAA AGGAAAAGAA AAAGAAGGAT GGGGAAAAAA AGGAGGCAAA GAAGACCAAG
    AAGGGTTCAG CAACTGGCGT CTCCCCTAAA CCAAAGAAAA AGAAGAAAAG TAAAGCGGAT
    AAAGTTGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54360
    Clone ID Related Accession (Same CDS sequence) XM_031898721.1
    Accession Version XM_031898721.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCGTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACACCT
    TCCACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGACTAAACC TGGAAAACTT
    GCAACACCAA CCAAGACACC AGTGATTTCT TTGCCGTTAG CAGAAAGTAG TGACTCGTCA
    GAAAGTGAAG ACGAGCCAGC AGTCAAGAAT GCACAACTAT CTGTCGTGGC GTCCAAGAAC
    AATAGCAAAA CGACTCTCTT GGCCAAGACA GGGAAGTTGG CTGCTCCAGC CAAGACGCCA
    GCGGTTACAC AGCCGCCAGT AGCCGAGAGC TCTGAGAGCA GCAACTCATC TGATAGTGAG
    GAGGAGCTTA ACAATAAGCA GAAGACTGCG GCACCTCCCC AGGCATCCCA GAAAAAGGGC
    AAATCGCCTC TCTTGGCCAG TACAGGGAAT GCTGCAACAC CAGCCAAGAC ACCAGTGGTT
    GCACAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCAACT CGTCAGACAG TGAGGAGGAG
    CCTGATGCTA AGAAAATAAA TGCATCCTTG AACCCTACAA CATCAGCGAG CCAAGCTTTT
    GCCACTATGA AGCCAACTGC CAAACAGGAC TGTGACAGTA GTGACACAAG TGAAGAAGCC
    GAGGGCAAAT CCAGGAGTCT GGTGGGGCAA CCATCGGTTA CCATTATCTC GGAGACCAGT
    GATACCACTG ATATTGATGA GGTAGTGTCT CCAACTCCTG CTCCAATCAA GGCAACCAAA
    GGAAAGGCTG TACCCATATT GGCAAAGACT GTCACACCAT CCAGCCTAAA AGCTACCAAA
    AGCAAAGCCA GCAGTGCAGC TAAAACTGCC AAAGCAGTGC AGCAAATAGC TGTAGCCAAC
    TCTGACAGCA GTGATTCTTT AGATAGCGAT GAGGATCCTG GAGTAAAGCA GACACCATCT
    GTACCATTAA AGAAAGTAAA GTCAGGGAAA GGAGCTAAAC TGACTGCCGT GTGTGTCAGT
    GGTGGAAATC AGAAACTGCC CAGTTCGTTA CCTGTGCCCT CTTTACAAAA GCCCAGTGTA
    TCTGACGTTT CTGAGCCCAG CGACTCGTCC GATAGTGACA TCGATACAGG AGCCCAGTCT
    CTTGTAGCAC CAGTACATGC GGTGCCCACC CCACAGAAAC GGAAGAGGAA TCCAAAAGTC
    AAAGCTCAAG TGACGCAACC TCCAGCTGCT TCAGTGAAAC ACAAAAAAAA GTCTAAAGCA
    GAGCAACTGG CAGCATCTGC ACAGCCAGCA CCAGAGCCAG TGATCGACTC TGAAGAGGAG
    ACCATAATGG CTCTCCTTTC GGGTCAGAGC CCAAAGAAGG GGAAGCAGAA ACTATCCAAA
    CTGGGACTGA AAGCAGCTCC TTCAGCCACT CCCCTTCCTG TACAAAAGAA TGGCAGTGTG
    TCGGACTCAT CCGATGACAG CGATATCGGA ACTGCAACAT TAAATATAGT TACACCTCCA
    GCGTTGGAGC TCAGGCCGCC GACCGCTGAC AAAGAAAAGA AAAGTGCAAA GAAGAGGAAG
    AGCTCCTCAG AAGATGGACC CAAAAAGAAA GCTAAGAAAG CAAAGAAGGA AGGAAAGAAA
    GAAAAGAAGA AATCAAAAGC AGAAGGAGGA GAGGCTGGAG CCAGCACCCC TAAAGCAAAG
    TCCTCCAAAA AAGATAAAAA GAAGCTAGAG AAATCAGAGA AAAAGAAATC AAGCAAAAAA
    AAAAAGGAAA AGAAAAAGAA GGATGGGGAA AAAAAGGAGG CAAAGAAGAC CAAGAAGGGT
    TCAGCAACTG GCGTCTCCCC TAAACCAAAG AAAAAGAAGA AAAGTAAAGC GGATAAAGTT
    GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754581.1
    CDS159..4364
    Translation

    Target ORF information:

    RefSeq Version XM_031898721.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898721.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCGTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACACCT
    TCCACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGACTAAACC TGGAAAACTT
    GCAACACCAA CCAAGACACC AGTGATTTCT TTGCCGTTAG CAGAAAGTAG TGACTCGTCA
    GAAAGTGAAG ACGAGCCAGC AGTCAAGAAT GCACAACTAT CTGTCGTGGC GTCCAAGAAC
    AATAGCAAAA CGACTCTCTT GGCCAAGACA GGGAAGTTGG CTGCTCCAGC CAAGACGCCA
    GCGGTTACAC AGCCGCCAGT AGCCGAGAGC TCTGAGAGCA GCAACTCATC TGATAGTGAG
    GAGGAGCTTA ACAATAAGCA GAAGACTGCG GCACCTCCCC AGGCATCCCA GAAAAAGGGC
    AAATCGCCTC TCTTGGCCAG TACAGGGAAT GCTGCAACAC CAGCCAAGAC ACCAGTGGTT
    GCACAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCAACT CGTCAGACAG TGAGGAGGAG
    CCTGATGCTA AGAAAATAAA TGCATCCTTG AACCCTACAA CATCAGCGAG CCAAGCTTTT
    GCCACTATGA AGCCAACTGC CAAACAGGAC TGTGACAGTA GTGACACAAG TGAAGAAGCC
    GAGGGCAAAT CCAGGAGTCT GGTGGGGCAA CCATCGGTTA CCATTATCTC GGAGACCAGT
    GATACCACTG ATATTGATGA GGTAGTGTCT CCAACTCCTG CTCCAATCAA GGCAACCAAA
    GGAAAGGCTG TACCCATATT GGCAAAGACT GTCACACCAT CCAGCCTAAA AGCTACCAAA
    AGCAAAGCCA GCAGTGCAGC TAAAACTGCC AAAGCAGTGC AGCAAATAGC TGTAGCCAAC
    TCTGACAGCA GTGATTCTTT AGATAGCGAT GAGGATCCTG GAGTAAAGCA GACACCATCT
    GTACCATTAA AGAAAGTAAA GTCAGGGAAA GGAGCTAAAC TGACTGCCGT GTGTGTCAGT
    GGTGGAAATC AGAAACTGCC CAGTTCGTTA CCTGTGCCCT CTTTACAAAA GCCCAGTGTA
    TCTGACGTTT CTGAGCCCAG CGACTCGTCC GATAGTGACA TCGATACAGG AGCCCAGTCT
    CTTGTAGCAC CAGTACATGC GGTGCCCACC CCACAGAAAC GGAAGAGGAA TCCAAAAGTC
    AAAGCTCAAG TGACGCAACC TCCAGCTGCT TCAGTGAAAC ACAAAAAAAA GTCTAAAGCA
    GAGCAACTGG CAGCATCTGC ACAGCCAGCA CCAGAGCCAG TGATCGACTC TGAAGAGGAG
    ACCATAATGG CTCTCCTTTC GGGTCAGAGC CCAAAGAAGG GGAAGCAGAA ACTATCCAAA
    CTGGGACTGA AAGCAGCTCC TTCAGCCACT CCCCTTCCTG TACAAAAGAA TGGCAGTGTG
    TCGGACTCAT CCGATGACAG CGATATCGGA ACTGCAACAT TAAATATAGT TACACCTCCA
    GCGTTGGAGC TCAGGCCGCC GACCGCTGAC AAAGAAAAGA AAAGTGCAAA GAAGAGGAAG
    AGCTCCTCAG AAGATGGACC CAAAAAGAAA GCTAAGAAAG CAAAGAAGGA AGGAAAGAAA
    GAAAAGAAGA AATCAAAAGC AGAAGGAGGA GAGGCTGGAG CCAGCACCCC TAAAGCAAAG
    TCCTCCAAAA AAGATAAAAA GAAGCTAGAG AAATCAGAGA AAAAGAAATC AAGCAAAAAA
    AAAAAGGAAA AGAAAAAGAA GGATGGGGAA AAAAAGGAGG CAAAGAAGAC CAAGAAGGGT
    TCAGCAACTG GCGTCTCCCC TAAACCAAAG AAAAAGAAGA AAAGTAAAGC GGATAAAGTT
    GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54361
    Clone ID Related Accession (Same CDS sequence) XM_031898722.1
    Accession Version XM_031898722.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCGTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAATAAATG CATCCTTGAA CCCTACAACA
    TCAGCGAGCC AAGCTTTTGC CACTATGAAG CCAACTGCCA AACAGGACTG TGACAGTAGT
    GACACAAGTG AAGAAGCCGA GGGCAAATCC AGGAGTCTGG TGGGGCAACC ATCGGTTACC
    ATTATCTCGG AGACCAGTGA TACCACTGAT ATTGATGAGG TAGTGTCTCC AACTCCTGCT
    CCAATCAAGG CAACCAAAGG AAAGGCTGTA CCCATATTGG CAAAGACTGT CACACCATCC
    AGCCTAAAAG CTACCAAAAG CAAAGCCAGC AGTGCAGCTA AAACTGCCAA AGCAGTGCAG
    CAAATAGCTG TAGCCAACTC TGACAGCAGT GATTCTTTAG ATAGCGATGA GGATCCTGGA
    GTAAAGCAGA CACCATCTGT ACCATTAAAG AAAGTAAAGT CAGGGAAAGG AGCTAAACTG
    ACTGCCGTGT GTGTCAGTGG TGGAAATCAG AAACTGCCCA GTTCGTTACC TGTGCCCTCT
    TTACAAAAGC CCAGTGTATC TGACGTTTCT GAGCCCAGCG ACTCGTCCGA TAGTGACATC
    GATACAGGAG CCCAGTCTCT TGTAGCACCA GTACATGCGG TGCCCACCCC ACAGAAACGG
    AAGAGGAATC CAAAAGTCAA AGCTCAAGTG ACGCAACCTC CAGCTGCTTC AGTGAAACAC
    AAAAAAAAGT CTAAAGCAGA GCAACTGGCA GCATCTGCAC AGCCAGCACC AGAGCCAGTG
    ATCGACTCTG AAGAGGAGAC CATAATGGCT CTCCTTTCGG GTCAGAGCCC AAAGAAGGGG
    AAGCAGAAAC TATCCAAACT GGGACTGAAA GCAGCTCCTT CAGCCACTCC CCTTCCTGTA
    CAAAAGAATG GCAGTGTGTC GGACTCATCC GATGACAGCG ATATCGGAAC TGCAACATTA
    AATATAGTTA CACCTCCAGC GTTGGAGCTC AGGCCGCCGA CCGCTGACAA AGAAAAGAAA
    AGTGCAAAGA AGAGGAAGAG CTCCTCAGAA GATGGACCCA AAAAGAAAGC TAAGAAAGCA
    AAGAAGGAAG GAAAGAAAGA AAAGAAGAAA TCAAAAGCAG AAGGAGGAGA GGCTGGAGCC
    AGCACCCCTA AAGCAAAGTC CTCCAAAAAA GATAAAAAGA AGCTAGAGAA ATCAGAGAAA
    AAGAAATCAA GCAAAAAAAA AAAGGAAAAG AAAAAGAAGG ATGGGGAAAA AAAGGAGGCA
    AAGAAGACCA AGAAGGGTTC AGCAACTGGC GTCTCCCCTA AACCAAAGAA AAAGAAGAAA
    AGTAAAGCGG ATAAAGTTGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754582.1
    CDS159..4022
    Translation

    Target ORF information:

    RefSeq Version XM_031898722.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898722.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCGTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAATAAATG CATCCTTGAA CCCTACAACA
    TCAGCGAGCC AAGCTTTTGC CACTATGAAG CCAACTGCCA AACAGGACTG TGACAGTAGT
    GACACAAGTG AAGAAGCCGA GGGCAAATCC AGGAGTCTGG TGGGGCAACC ATCGGTTACC
    ATTATCTCGG AGACCAGTGA TACCACTGAT ATTGATGAGG TAGTGTCTCC AACTCCTGCT
    CCAATCAAGG CAACCAAAGG AAAGGCTGTA CCCATATTGG CAAAGACTGT CACACCATCC
    AGCCTAAAAG CTACCAAAAG CAAAGCCAGC AGTGCAGCTA AAACTGCCAA AGCAGTGCAG
    CAAATAGCTG TAGCCAACTC TGACAGCAGT GATTCTTTAG ATAGCGATGA GGATCCTGGA
    GTAAAGCAGA CACCATCTGT ACCATTAAAG AAAGTAAAGT CAGGGAAAGG AGCTAAACTG
    ACTGCCGTGT GTGTCAGTGG TGGAAATCAG AAACTGCCCA GTTCGTTACC TGTGCCCTCT
    TTACAAAAGC CCAGTGTATC TGACGTTTCT GAGCCCAGCG ACTCGTCCGA TAGTGACATC
    GATACAGGAG CCCAGTCTCT TGTAGCACCA GTACATGCGG TGCCCACCCC ACAGAAACGG
    AAGAGGAATC CAAAAGTCAA AGCTCAAGTG ACGCAACCTC CAGCTGCTTC AGTGAAACAC
    AAAAAAAAGT CTAAAGCAGA GCAACTGGCA GCATCTGCAC AGCCAGCACC AGAGCCAGTG
    ATCGACTCTG AAGAGGAGAC CATAATGGCT CTCCTTTCGG GTCAGAGCCC AAAGAAGGGG
    AAGCAGAAAC TATCCAAACT GGGACTGAAA GCAGCTCCTT CAGCCACTCC CCTTCCTGTA
    CAAAAGAATG GCAGTGTGTC GGACTCATCC GATGACAGCG ATATCGGAAC TGCAACATTA
    AATATAGTTA CACCTCCAGC GTTGGAGCTC AGGCCGCCGA CCGCTGACAA AGAAAAGAAA
    AGTGCAAAGA AGAGGAAGAG CTCCTCAGAA GATGGACCCA AAAAGAAAGC TAAGAAAGCA
    AAGAAGGAAG GAAAGAAAGA AAAGAAGAAA TCAAAAGCAG AAGGAGGAGA GGCTGGAGCC
    AGCACCCCTA AAGCAAAGTC CTCCAAAAAA GATAAAAAGA AGCTAGAGAA ATCAGAGAAA
    AAGAAATCAA GCAAAAAAAA AAAGGAAAAG AAAAAGAAGG ATGGGGAAAA AAAGGAGGCA
    AAGAAGACCA AGAAGGGTTC AGCAACTGGC GTCTCCCCTA AACCAAAGAA AAAGAAGAAA
    AGTAAAGCGG ATAAAGTTGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54362
    Clone ID Related Accession (Same CDS sequence) XM_031898723.1
    Accession Version XM_031898723.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGTCTC TCCAAGGATC CCAGAAAAAT
    ACACCAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGAAATTTG
    GCAACACCAG CCAAGGCATC TATGGTTGCT CAGCCACCGG CAGCTCAGAG CTCTGAGAGC
    AGCGACTTGT CAGACAGTGA GGAGGAACCT AATGCTAAGA AAACAGCAGC GCCTCTCCAG
    GGATCCCAGA AAAAGACACC TGCCACAGCC GCTAGGCCAC CAGAGACAAA AGCACCCCTC
    TTGGCCAAAC CTGGAAAACT GGCAACACCC ACCAAGGCAT CTGTGGTTGC ACAGCCACTG
    GCAGCTCAGA GCTCTGAGAG CAGCGACTCG TCAGACAGTG AGGAGGAGCC TGATGCTAAG
    AAAACAGCAG CGCCTCTCCA GGGATCCCAG AAAAAGACAC CTGCCACACC TTCCACAGCC
    ACTAGGCCAC CAGGGACAAA AACACCCCTC TTGACTAAAC CTGGAAAACT TGCAACACCA
    ACCAAGACAC CAGTGATTTC TTTGCCGTTA GCAGAAAGTA GTGACTCGTC AGAAAGTGAA
    GACGAGCCAG CAGTCAAGAA TGCACAACTA TCTGTCGTGG CGTCCAAGAA CAATAGCAAA
    ACGACTCTCT TGGCCAAGAC AGGGAAGTTG GCTGCTCCAG CCAAGACGCC AGCGGTTACA
    CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC AGCAACTCAT CTGATAGTGA GGAGGAGCTT
    AACAATAAGC AGAAGACTGC GGCACCTCCC CAGGCATCCC AGAAAAAGGG CAAATCGCCT
    CTCTTGGCCA GTACAGGGAA TGCTGCAACA CCAGCCAAGA CACCAGTGGT TGCACAGCCC
    CTAACAGCTC AGAGCTCTGA GAGCAGCAAC TCGTCAGACA GTGAGGAGGA GCCTGATGCT
    AAGAAAATAA ATGCATCCTT GAACCCTACA ACATCAGCGA GCCAAGCTTT TGCCACTATG
    AAGCCAACTG CCAAACAGGA CTGTGACAGT AGTGACACAA GTGAAGAAGC CGAGGGCAAA
    TCCAGGAGTC TGGTGGGGCA ACCATCGGTT ACCATTATCT CGGAGACCAG TGATACCACT
    GATATTGATG AGGTAGTGTC TCCAACTCCT GCTCCAATCA AGGCAACCAA AGGAAAGGCT
    GTACCCATAT TGGCAAAGAC TGTCACACCA TCCAGCCTAA AAGCTACCAA AAGCAAAGCC
    AGCAGTGCAG CTAAAACTGC CAAAGCAGTG CAGCAAATAG CTGTAGCCAA CTCTGACAGC
    AGTGATTCTT TAGATAGCGA TGAGGATCCT GGAGTAAAGC AGACACCATC TGTACCATTA
    AAGAAAGTAA AGTCAGGGAA AGGAGCTAAA CTGACTGCCG TGTGTGTCAG TGGTGGAAAT
    CAGAAACTGC CCAGTTCGTT ACCTGTGCCC TCTTTACAAA AGCCCAGTGT ATCTGACGTT
    TCTGAGCCCA GCGACTCGTC CGATAGTGAC ATCGATACAG GAGCCCAGTC TCTTGTAGCA
    CCAGTACATG CGGTGCCCAC CCCACAGAAA CGGAAGAGGA ATCCAAAAGT CAAAGCTCAA
    GTGACGCAAC CTCCAGCTGC TTCAGTGAAA CACAAAAAAA AGTCTAAAGC AGAGCAACTG
    GCAGCATCTG CACAGCCAGC ACCAGAGCCA GTGATCGACT CTGAAGAGGA GACCATAATG
    GCTCTCCTTT CGGGTCAGAG CCCAAAGAAG GGGAAGCAGA AACTATCCAA ACTGGGACTG
    AAAGCAGCTC CTTCAGCCAC TCCCCTTCCT GTACAAAAGA ATGGCAGTGT GTCGGACTCA
    TCCGATGACA GCGATATCGG AACTGCAACA TTAAATATAG TTACACCTCC AGCGTTGGAG
    CTCAGGCCGC CGACCGCTGA CAAAGAAAAG AAAAGTGCAA AGAAGAGGAA GAGCTCCTCA
    GAAGATGGAC CCAAAAAGAA AGCTAAGAAA GCAAAGAAGG AAGGAAAGAA AGAAAAGAAG
    AAATCAAAAG CAGAAGGAGG AGAGGCTGGA GCCAGCACCC CTAAAGCAAA GTCCTCCAAA
    AAAGATAAAA AGAAGCTAGA GAAATCAGAG AAAAAGAAAT CAAGCAAAAA AAAAAAGGAA
    AAGAAAAAGA AGGATGGGGA AAAAAAGGAG GCAAAGAAGA CCAAGAAGGG TTCAGCAACT
    GGCGTCTCCC CTAAACCAAA GAAAAAGAAG AAAAGTAAAG CGGATAAAGT TGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754583.1
    CDS160..3756
    Translation

    Target ORF information:

    RefSeq Version XM_031898723.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898723.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGTCTC TCCAAGGATC CCAGAAAAAT
    ACACCAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGAAATTTG
    GCAACACCAG CCAAGGCATC TATGGTTGCT CAGCCACCGG CAGCTCAGAG CTCTGAGAGC
    AGCGACTTGT CAGACAGTGA GGAGGAACCT AATGCTAAGA AAACAGCAGC GCCTCTCCAG
    GGATCCCAGA AAAAGACACC TGCCACAGCC GCTAGGCCAC CAGAGACAAA AGCACCCCTC
    TTGGCCAAAC CTGGAAAACT GGCAACACCC ACCAAGGCAT CTGTGGTTGC ACAGCCACTG
    GCAGCTCAGA GCTCTGAGAG CAGCGACTCG TCAGACAGTG AGGAGGAGCC TGATGCTAAG
    AAAACAGCAG CGCCTCTCCA GGGATCCCAG AAAAAGACAC CTGCCACACC TTCCACAGCC
    ACTAGGCCAC CAGGGACAAA AACACCCCTC TTGACTAAAC CTGGAAAACT TGCAACACCA
    ACCAAGACAC CAGTGATTTC TTTGCCGTTA GCAGAAAGTA GTGACTCGTC AGAAAGTGAA
    GACGAGCCAG CAGTCAAGAA TGCACAACTA TCTGTCGTGG CGTCCAAGAA CAATAGCAAA
    ACGACTCTCT TGGCCAAGAC AGGGAAGTTG GCTGCTCCAG CCAAGACGCC AGCGGTTACA
    CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC AGCAACTCAT CTGATAGTGA GGAGGAGCTT
    AACAATAAGC AGAAGACTGC GGCACCTCCC CAGGCATCCC AGAAAAAGGG CAAATCGCCT
    CTCTTGGCCA GTACAGGGAA TGCTGCAACA CCAGCCAAGA CACCAGTGGT TGCACAGCCC
    CTAACAGCTC AGAGCTCTGA GAGCAGCAAC TCGTCAGACA GTGAGGAGGA GCCTGATGCT
    AAGAAAATAA ATGCATCCTT GAACCCTACA ACATCAGCGA GCCAAGCTTT TGCCACTATG
    AAGCCAACTG CCAAACAGGA CTGTGACAGT AGTGACACAA GTGAAGAAGC CGAGGGCAAA
    TCCAGGAGTC TGGTGGGGCA ACCATCGGTT ACCATTATCT CGGAGACCAG TGATACCACT
    GATATTGATG AGGTAGTGTC TCCAACTCCT GCTCCAATCA AGGCAACCAA AGGAAAGGCT
    GTACCCATAT TGGCAAAGAC TGTCACACCA TCCAGCCTAA AAGCTACCAA AAGCAAAGCC
    AGCAGTGCAG CTAAAACTGC CAAAGCAGTG CAGCAAATAG CTGTAGCCAA CTCTGACAGC
    AGTGATTCTT TAGATAGCGA TGAGGATCCT GGAGTAAAGC AGACACCATC TGTACCATTA
    AAGAAAGTAA AGTCAGGGAA AGGAGCTAAA CTGACTGCCG TGTGTGTCAG TGGTGGAAAT
    CAGAAACTGC CCAGTTCGTT ACCTGTGCCC TCTTTACAAA AGCCCAGTGT ATCTGACGTT
    TCTGAGCCCA GCGACTCGTC CGATAGTGAC ATCGATACAG GAGCCCAGTC TCTTGTAGCA
    CCAGTACATG CGGTGCCCAC CCCACAGAAA CGGAAGAGGA ATCCAAAAGT CAAAGCTCAA
    GTGACGCAAC CTCCAGCTGC TTCAGTGAAA CACAAAAAAA AGTCTAAAGC AGAGCAACTG
    GCAGCATCTG CACAGCCAGC ACCAGAGCCA GTGATCGACT CTGAAGAGGA GACCATAATG
    GCTCTCCTTT CGGGTCAGAG CCCAAAGAAG GGGAAGCAGA AACTATCCAA ACTGGGACTG
    AAAGCAGCTC CTTCAGCCAC TCCCCTTCCT GTACAAAAGA ATGGCAGTGT GTCGGACTCA
    TCCGATGACA GCGATATCGG AACTGCAACA TTAAATATAG TTACACCTCC AGCGTTGGAG
    CTCAGGCCGC CGACCGCTGA CAAAGAAAAG AAAAGTGCAA AGAAGAGGAA GAGCTCCTCA
    GAAGATGGAC CCAAAAAGAA AGCTAAGAAA GCAAAGAAGG AAGGAAAGAA AGAAAAGAAG
    AAATCAAAAG CAGAAGGAGG AGAGGCTGGA GCCAGCACCC CTAAAGCAAA GTCCTCCAAA
    AAAGATAAAA AGAAGCTAGA GAAATCAGAG AAAAAGAAAT CAAGCAAAAA AAAAAAGGAA
    AAGAAAAAGA AGGATGGGGA AAAAAAGGAG GCAAAGAAGA CCAAGAAGGG TTCAGCAACT
    GGCGTCTCCC CTAAACCAAA GAAAAAGAAG AAAAGTAAAG CGGATAAAGT TGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa21469
    Clone ID Related Accession (Same CDS sequence) NM_001142109.1
    Accession Version NM_001142109.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2976bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-10-28
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC168088.1. ##Evidence-Data-START## Transcript exon combination :: BC168088.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1025683, SAMEA1025684 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA TTTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    GCAACACCAG CCAAGGCATC TGTAGTTGCT CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCTTCTC TCCAAGGATC CCAGAAAAAT
    ACACCAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGAAATTTG
    GCAACACCAG CCAAGGCATC TATGGTTGCT CAGCCACCGG CAGCTCAGAG CTCTGAGAGC
    AGCGACTTGT CAGACAGTGA GGAGGAACCT AATGCTAAGA AAACAGCAGC GCCTCTCCAG
    GGATCCCAGA AAAAGACACC TGCCACAGCC GCTAGGCCAC CAGAGACAAA AGCACCCCTC
    TTGGCCAAAC CTGGAAAACT GGCAACACCC ACCAAGGCAT CTGTGGTTGC ACAGCCACTG
    GCAGCTCAGA GCTCTGAGAG CAGCGACTCG TCAGACAGTG AGGAGGAGCC TGATGCTAAG
    AAAACAGCAG CGCCTCTCCA GGGATCCCAG AAAAAGACAC TTGCCACACC TTCCACAGCC
    ACTAGGCCAC CAGGGACAAA AACACCCCTC TTGACTAAAC CTGGAAAACT TGCAACACCA
    ACCAAGACAC CAGTGATTTC TTTGCCGTTA GCAGAAAGTA GTGACTCGTC AGAAAGTGAA
    GACGAGCCAG CAGTCAAGAA TGCACAACTA TCTGTCGTGG CGTCCAAGAA CAATAGCAAA
    ACGACTCTCT TGGCCAAGAC AGGGAAGTTG GCTGCTCCAG CCAAGACGCC AGCGGTTACA
    CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC AGCAACTCAT CTGATAGTGA GGAGGAGCTT
    AACAATAAGA AGACTGCGGC ACCTCCCCAG GCATCCCAGA AAAAGGGCAA ATCGCCTCTC
    TTGGCCAGTA CAGGGAATGC TGCAACACCA GCCAAGACAC CAGTGGTTGC ACAGCCCCTA
    ACAGCTCAGA GCTCTGAGAG CAGCAACTCG TCAGACAGTG AGGAGGAGCC TGATGCTAAG
    GTAGTGTCTC CAACTCCTGC TCCAATCAAG GCAACCAAAG GAAAGGCTGT ACCCATATTG
    GCAAAGACTG TCACACCATC CAGCCAGCAC CCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001135581.1
    CDS115..3090
    Translation

    Target ORF information:

    RefSeq Version NM_001142109.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001142109.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA TTTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    GCAACACCAG CCAAGGCATC TGTAGTTGCT CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCTTCTC TCCAAGGATC CCAGAAAAAT
    ACACCAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGAAATTTG
    GCAACACCAG CCAAGGCATC TATGGTTGCT CAGCCACCGG CAGCTCAGAG CTCTGAGAGC
    AGCGACTTGT CAGACAGTGA GGAGGAACCT AATGCTAAGA AAACAGCAGC GCCTCTCCAG
    GGATCCCAGA AAAAGACACC TGCCACAGCC GCTAGGCCAC CAGAGACAAA AGCACCCCTC
    TTGGCCAAAC CTGGAAAACT GGCAACACCC ACCAAGGCAT CTGTGGTTGC ACAGCCACTG
    GCAGCTCAGA GCTCTGAGAG CAGCGACTCG TCAGACAGTG AGGAGGAGCC TGATGCTAAG
    AAAACAGCAG CGCCTCTCCA GGGATCCCAG AAAAAGACAC TTGCCACACC TTCCACAGCC
    ACTAGGCCAC CAGGGACAAA AACACCCCTC TTGACTAAAC CTGGAAAACT TGCAACACCA
    ACCAAGACAC CAGTGATTTC TTTGCCGTTA GCAGAAAGTA GTGACTCGTC AGAAAGTGAA
    GACGAGCCAG CAGTCAAGAA TGCACAACTA TCTGTCGTGG CGTCCAAGAA CAATAGCAAA
    ACGACTCTCT TGGCCAAGAC AGGGAAGTTG GCTGCTCCAG CCAAGACGCC AGCGGTTACA
    CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC AGCAACTCAT CTGATAGTGA GGAGGAGCTT
    AACAATAAGA AGACTGCGGC ACCTCCCCAG GCATCCCAGA AAAAGGGCAA ATCGCCTCTC
    TTGGCCAGTA CAGGGAATGC TGCAACACCA GCCAAGACAC CAGTGGTTGC ACAGCCCCTA
    ACAGCTCAGA GCTCTGAGAG CAGCAACTCG TCAGACAGTG AGGAGGAGCC TGATGCTAAG
    GTAGTGTCTC CAACTCCTGC TCCAATCAAG GCAACCAAAG GAAAGGCTGT ACCCATATTG
    GCAAAGACTG TCACACCATC CAGCCAGCAC CCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32615
    Clone ID Related Accession (Same CDS sequence) XM_012958673.2
    Accession Version XM_012958673.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC TTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGC AGAAGACTGC GGCACCTCCC
    CAGGCATCCC AGAAAAAGGG CAAATCGCCT CTCTTGGCCA GTACAGGGAA TGCTGCAACA
    CCAGCCAAGA CACCAGTGGT TGCACAGCCC CTAACAGCTC AGAGCTCTGA GAGCAGCAAC
    TCGTCAGACA GTGAGGAGGA GCCTGATGCT AAGAAAATAA ATGCATCCTT GAACCCTACA
    ACATCAGCGA GCCAAGCTTT TGCCACTATG AAGCCAACTG CCAAACAGGA CTGTGACAGT
    AGTGACACAA GTGAAGAAGC CGAGGGCAAA TCCAGGAGTC TGGTGGGGCA ACCATCGGTT
    ACCATTATCT CGGAGACCAG TGATACCACT GATATTGATG AGGTAGTGTC TCCAACTCCT
    GCTCCAATCA AGGCAACCAA AGGAAAGGCT GTACCCATAT TGGCAAAGAC TGTCACACCA
    TCCAGCCTAA AAGCTACCAA AAGCAAAGCC AGCAGTGCAG CTAAAACTGC CAAAGCAGTG
    CAGCAAATAG CTGTAGCCAA CTCTGACAGC AGTGATTCTT TAGATAGCGA TGAGGATCCT
    GGAGTAAAGC AGACACCATC TGTACCATTA AAGAAAGTAA AGTCAGGGAA AGGAGCTAAA
    CTGACTGCCG TGTGTGTCAG TGGTGGAAAT CAGAAACTGC CCAGTTCGTT ACCTGTGCCC
    TCTTTACAAA AGCCCAGTGT ATCTGACGTT TCTGAGCCCA GCGACTCGTC CGATAGTGAC
    ATCGATACAG GAGCCCAGTC TCTTGTAGCA CCAGTACATG CGGTGCCCAC CCCACAGAAA
    CGGAAGAGGA ATCCAAAAGT CAAAGCTCAA GTGACGCAAC CTCCAGCTGC TTCAGTGAAA
    CACAAAAAAA AGTCTAAAGC AGAGCAACTG GCAGCATCTG CACAGCCAGC ACCAGAGCCA
    GTGATCGACT CTGAAGAGGA GACCATAATG GCTCTCCTTT CGGGTCAGAG CCCAAAGAAG
    GGGAAGCAGA AACTATCCAA ACTGGGACTG AAAGCAGCTC CTTCAGCCAC TCCCCTTCCT
    GTACAAAAGA ATGGCAGTGT GTCGGACTCA TCCGATGACA GCGATATCGG AACTGCAACA
    TTAAATATAG TTACACCTCC AGCGTTGGAG CTCAGGCCGC CGACCGCTGA CAAAGAAAAG
    AAAAGTGCAA AGAAGAGGAA GAGCTCCTCA GAAGATGGAC CCAAAAAGAA AGCTAAGAAA
    GCAAAGAAGG AAGGAAAGAA AGAAAAGAAG AAATCAAAAG CAGAAGGAGG AGAGGCTGGA
    GCCAGCACCC CTAAAGCAAA GTCCTCCAAA AAAGATAAAA AGAAGCTAGA GAAATCAGAG
    AAAAAGAAAT CAAGCAAAAA AAAAAAAAGG AAAAGAAAAA GAAGGATGGG GAAAAAAAGG
    AGGCAAAGAA GACCAAGAAG GGTTCAGCAA CTGGCGTCTC CCCTAAACCA AAGAAAAAGA
    AGAAAAGTAA AGCGGATAAA GTTGACTGAG GAACACAAGG AAGGAGGGCC TGATATCTAC
    CCGCCAGGTA CAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814127.1
    CDS162..4619
    Translation

    Target ORF information:

    RefSeq Version XM_012958673.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958673.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC TTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGC AGAAGACTGC GGCACCTCCC
    CAGGCATCCC AGAAAAAGGG CAAATCGCCT CTCTTGGCCA GTACAGGGAA TGCTGCAACA
    CCAGCCAAGA CACCAGTGGT TGCACAGCCC CTAACAGCTC AGAGCTCTGA GAGCAGCAAC
    TCGTCAGACA GTGAGGAGGA GCCTGATGCT AAGAAAATAA ATGCATCCTT GAACCCTACA
    ACATCAGCGA GCCAAGCTTT TGCCACTATG AAGCCAACTG CCAAACAGGA CTGTGACAGT
    AGTGACACAA GTGAAGAAGC CGAGGGCAAA TCCAGGAGTC TGGTGGGGCA ACCATCGGTT
    ACCATTATCT CGGAGACCAG TGATACCACT GATATTGATG AGGTAGTGTC TCCAACTCCT
    GCTCCAATCA AGGCAACCAA AGGAAAGGCT GTACCCATAT TGGCAAAGAC TGTCACACCA
    TCCAGCCTAA AAGCTACCAA AAGCAAAGCC AGCAGTGCAG CTAAAACTGC CAAAGCAGTG
    CAGCAAATAG CTGTAGCCAA CTCTGACAGC AGTGATTCTT TAGATAGCGA TGAGGATCCT
    GGAGTAAAGC AGACACCATC TGTACCATTA AAGAAAGTAA AGTCAGGGAA AGGAGCTAAA
    CTGACTGCCG TGTGTGTCAG TGGTGGAAAT CAGAAACTGC CCAGTTCGTT ACCTGTGCCC
    TCTTTACAAA AGCCCAGTGT ATCTGACGTT TCTGAGCCCA GCGACTCGTC CGATAGTGAC
    ATCGATACAG GAGCCCAGTC TCTTGTAGCA CCAGTACATG CGGTGCCCAC CCCACAGAAA
    CGGAAGAGGA ATCCAAAAGT CAAAGCTCAA GTGACGCAAC CTCCAGCTGC TTCAGTGAAA
    CACAAAAAAA AGTCTAAAGC AGAGCAACTG GCAGCATCTG CACAGCCAGC ACCAGAGCCA
    GTGATCGACT CTGAAGAGGA GACCATAATG GCTCTCCTTT CGGGTCAGAG CCCAAAGAAG
    GGGAAGCAGA AACTATCCAA ACTGGGACTG AAAGCAGCTC CTTCAGCCAC TCCCCTTCCT
    GTACAAAAGA ATGGCAGTGT GTCGGACTCA TCCGATGACA GCGATATCGG AACTGCAACA
    TTAAATATAG TTACACCTCC AGCGTTGGAG CTCAGGCCGC CGACCGCTGA CAAAGAAAAG
    AAAAGTGCAA AGAAGAGGAA GAGCTCCTCA GAAGATGGAC CCAAAAAGAA AGCTAAGAAA
    GCAAAGAAGG AAGGAAAGAA AGAAAAGAAG AAATCAAAAG CAGAAGGAGG AGAGGCTGGA
    GCCAGCACCC CTAAAGCAAA GTCCTCCAAA AAAGATAAAA AGAAGCTAGA GAAATCAGAG
    AAAAAGAAAT CAAGCAAAAA AAAAAAAAGG AAAAGAAAAA GAAGGATGGG GAAAAAAAGG
    AGGCAAAGAA GACCAAGAAG GGTTCAGCAA CTGGCGTCTC CCCTAAACCA AAGAAAAAGA
    AGAAAAGTAA AGCGGATAAA GTTGACTGAG GAACACAAGG AAGGAGGGCC TGATATCTAC
    CCGCCAGGTA CAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32616
    Clone ID Related Accession (Same CDS sequence) XM_012958674.2
    Accession Version XM_012958674.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958674.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC TTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGC AGAAGACTGC GGCACCTCCC
    CAGGCATCCC AGAAAAAGGG CAAATCGCCT CTCTTGGCCA GTACAGGGAA TGCTGCAACA
    CCAGCCAAGA CACCAGTGGT TGCACAGCCC CTAACAGCTC AGAGCTCTGA GAGCAGCAAC
    TCGTCAGACA GTGAGGAGGA GCCTGATGCT AAGAAAATAA ATGCATCCTT GAACCCTACA
    ACATCAGCGA GCCAAGCTTT TGCCACTATG AAGCCAACTG CCAAACAGGA CTGTGACAGT
    AGTGACACAA GTGAAGAAGC CGAGGGCAAA TCCAGGAGTC TGGTGGGGCA ACCATCGGTT
    ACCATTATCT CGGAGACCAG TGATACCACT GATATTGATG AGGTAGTGTC TCCAACTCCT
    GCTCCAATCA AGGCAACCAA AGGAAAGGCT GTACCCATAT TGGCAAAGAC TGTCACACCA
    TCCAGCCTAA AAGCTACCAA AAGCAAAGCC AGCAGTGCAG CTAAAACTGC CAAAGCAGTG
    CAGCAAATAG CTGTAGCCAA CTCTGACAGC AGTGATTCTT TAGATAGCGA TGAGGATCCT
    GGAGTAAAGC AGACACCATC TGTACCATTA AAGAAAGTAA AGTCAGGGAA AGGAGCTAAA
    CTGACTGCCG TGTGTGTCAG TGGTGGAAAT CAGAAACTGC CCAGTTCGTT ACCTGTGCCC
    TCTTTACAAA AGCCCAGTGT ATCTGACGTT TCTGAGCCCA GCGACTCGTC CGATAGTGAC
    ATCGATACAG GAGCCCAGTC TCTTGTAGCA CCAGTACATG CGGTGCCCAC CCCACAGAAA
    CGGAAGAGGA ATCCAAAAGT CAAAGCTCAA GTGACGCAAC CTCCAGCTGC TTCAGTGAAA
    CACAAAAAAA AGTCTAAAGC AGAGCAACTG GCAGCATCTG CACAGCCAGC ACCAGAGCCA
    GTGATCGACT CTGAAGAGGA GACCATAATG GCTCTCCTTT CGGGTCAGAG CCCAAAGAAG
    GGGAAGCAGA AACTATCCAA ACTGGGACTG AAAGCAGCTC CTTCAGCCAC TCCCCTTCCT
    GTACAAAAGA ATGGCAGTGT GTCGGACTCA TCCGATGACA GCGATATCGG AACTGCAACA
    TTAAATATAG TTACACCTCC AGCGTTGGAG CTCAGGCCGC CGACCGCTGA CAAAGAAAAG
    AAAAGTGCAA AGAAGAGGAA GAGCTCCTCA GAAGATGGAC CCAAAAAGAA AGCTAAGAAA
    GCAAAGAAGG AAGGAAAGAA AGAAAAGAAG AAATCAAAAG CAGAAGGAGG AGAGGCTGGA
    GCCAGCACCC CTAAAGCAAA GTCCTCCAAA AAAGATAAAA AGAAGCTAGA GAAATCAGAG
    AAAAAGAAAT CAAGCAAAAA AAAAAAGGAA AAGAAAAAGA AGGATGGGGA AAAAAAGGAG
    GCAAAGAAGA CCAAGAAGGG TTCAGCAACT GGCGTCTCCC CTAAACCAAA GAAAAAGAAG
    AAAAGTAAAG CGGATAAAGT TGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814128.1
    CDS162..4568
    Translation

    Target ORF information:

    RefSeq Version XM_012958674.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958674.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC TTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGC AGAAGACTGC GGCACCTCCC
    CAGGCATCCC AGAAAAAGGG CAAATCGCCT CTCTTGGCCA GTACAGGGAA TGCTGCAACA
    CCAGCCAAGA CACCAGTGGT TGCACAGCCC CTAACAGCTC AGAGCTCTGA GAGCAGCAAC
    TCGTCAGACA GTGAGGAGGA GCCTGATGCT AAGAAAATAA ATGCATCCTT GAACCCTACA
    ACATCAGCGA GCCAAGCTTT TGCCACTATG AAGCCAACTG CCAAACAGGA CTGTGACAGT
    AGTGACACAA GTGAAGAAGC CGAGGGCAAA TCCAGGAGTC TGGTGGGGCA ACCATCGGTT
    ACCATTATCT CGGAGACCAG TGATACCACT GATATTGATG AGGTAGTGTC TCCAACTCCT
    GCTCCAATCA AGGCAACCAA AGGAAAGGCT GTACCCATAT TGGCAAAGAC TGTCACACCA
    TCCAGCCTAA AAGCTACCAA AAGCAAAGCC AGCAGTGCAG CTAAAACTGC CAAAGCAGTG
    CAGCAAATAG CTGTAGCCAA CTCTGACAGC AGTGATTCTT TAGATAGCGA TGAGGATCCT
    GGAGTAAAGC AGACACCATC TGTACCATTA AAGAAAGTAA AGTCAGGGAA AGGAGCTAAA
    CTGACTGCCG TGTGTGTCAG TGGTGGAAAT CAGAAACTGC CCAGTTCGTT ACCTGTGCCC
    TCTTTACAAA AGCCCAGTGT ATCTGACGTT TCTGAGCCCA GCGACTCGTC CGATAGTGAC
    ATCGATACAG GAGCCCAGTC TCTTGTAGCA CCAGTACATG CGGTGCCCAC CCCACAGAAA
    CGGAAGAGGA ATCCAAAAGT CAAAGCTCAA GTGACGCAAC CTCCAGCTGC TTCAGTGAAA
    CACAAAAAAA AGTCTAAAGC AGAGCAACTG GCAGCATCTG CACAGCCAGC ACCAGAGCCA
    GTGATCGACT CTGAAGAGGA GACCATAATG GCTCTCCTTT CGGGTCAGAG CCCAAAGAAG
    GGGAAGCAGA AACTATCCAA ACTGGGACTG AAAGCAGCTC CTTCAGCCAC TCCCCTTCCT
    GTACAAAAGA ATGGCAGTGT GTCGGACTCA TCCGATGACA GCGATATCGG AACTGCAACA
    TTAAATATAG TTACACCTCC AGCGTTGGAG CTCAGGCCGC CGACCGCTGA CAAAGAAAAG
    AAAAGTGCAA AGAAGAGGAA GAGCTCCTCA GAAGATGGAC CCAAAAAGAA AGCTAAGAAA
    GCAAAGAAGG AAGGAAAGAA AGAAAAGAAG AAATCAAAAG CAGAAGGAGG AGAGGCTGGA
    GCCAGCACCC CTAAAGCAAA GTCCTCCAAA AAAGATAAAA AGAAGCTAGA GAAATCAGAG
    AAAAAGAAAT CAAGCAAAAA AAAAAAGGAA AAGAAAAAGA AGGATGGGGA AAAAAAGGAG
    GCAAAGAAGA CCAAGAAGGG TTCAGCAACT GGCGTCTCCC CTAAACCAAA GAAAAAGAAG
    AAAAGTAAAG CGGATAAAGT TGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32617
    Clone ID Related Accession (Same CDS sequence) XM_012958675.2
    Accession Version XM_012958675.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958675.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA CACATACAGT TTTGGCTCAT GTTCATGCAC CACCAAGGAC ACCTCTGCTA
    TCTCCTTCCA AAACTGCAAG GGCTGCTGTG CCAGCAAAAG CTGCTGTTGT CACTAAACCA
    GTAGATATCG ATGAGTCGGA GAGCAGTGAT TCATCGGACA GTGAAATCAT AGTTACCCAG
    GCATCCCAGA AAAAGCCCAG TGATCCTACA GCCGCTAAGC CACTAGTGAC AAAAACACCC
    AACTTGGCCC AACCTGGAAA GGTGGCAACG CCAACAAAGA CACCAGTGAG TGCAAAGCCC
    CTAACAGCTC AGAGCTCTGA GAGCAGCGAC TCGTCAGACA GTGAGGAGGA GCTTAATGCT
    AAGAATACAG CAGCACCTCT CCAGGCATCC CAGAAAAAGA CTATTGTTCC TCCCACATCA
    ACAAGGCCAC CAGGGACAAA AACACCCCTC TTGGCCAAAC CCGGAACATT GACAACACCA
    GCCAAGGCAT CTGTGGTTGC TCAGAGCTCT GAGAGCAGCG ACTCGTCAGA CAGTGAGTTG
    GAGCCTGATG CTAAGAAAAC AGCAGCGCCT CTCCAAGGAT CCCAAAAAAA TATACCTGCC
    ACACCTTCTA CAGCCACTAG GCCACCAGGG ACAAAAACAC CCCTCTTGGC CAAACCTGGG
    AAACTGGCAA CACCAACCAA GGCATCTGTG GTTGCTAAGC CACTGGCAGC TCAAAGCTCT
    GAGAGCAGTG ACTCGTCCGA CAGTGAGGAG GAGCCTGATA CTAAGAAGAC AGCCGTGCCT
    CTCCAGGGAT CTCAGAAAAA AACACCTGTC ACACCTTCCA CAGCCGCTAG ACCACCAGGG
    ACAAAAACAC CCCTCTTGGC CAAACCCGGA ACATTGGCAA CACCAGCCAA AGCATCTGTA
    GTTCCTCAGA GCTCTGAGAG CAGTGACTCG TCAGACAGTG AGTTGGAGCC TGATGCTAAG
    AAAACAGCAG CGCCTCTCCA AGGATCCCAA AAAAATATAC CTGCCACACC TTCTACAGCC
    ACTAGGCCAC CAGGGACAAA AACACCCCTC TTGGCCAAAC CTGGGAAACT GGCAACACCA
    ACCAAGGCAT CTGTGGTTGC TCAGCCACTG GCAGCTCAAA GCTCTGAGAG CAGCGACTCG
    TCCGACAGTG AGGAGGAGCC TGATACTAAG AAGACAGCCG TGCCTCTCCA GGGATCTCAG
    AAAAAAACAC CTGTCACACC TTCCACAGCC GCTAGACCAC CAGGGACAAA AACACCCCTC
    TTGGCCAAAC CCGGAACATT GGCAACACCA GCCAAGGCAT CTGTAGTTGC TCAGAGCTCT
    GAGAGCAGTG ACTCGTCAGA CAGTGAGTTG GAGCCTGATG CTAAGAAAAC AGCAGCTTCT
    CTCCAAGGAT CCCAGAAAAA TACACCAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGGCCAAAC CTGGAAATTT GGCAACACCA GCCAAGGCAT CTATGGTTGC TCAGCCACCG
    GCAGCTCAGA GCTCTGAGAG CAGCGACTTG TCAGACAGTG AGGAGGAACC TAATGCTAAG
    AAAACAGCAG CGCCTCTCCA GGGATCCCAG AAAAAGACAC CTGCCACAGC CGCTAGGCCA
    CCAGAGACAA AAGCACCCCT CTTGGCCAAA CCTGGAAAAC TGGCAACACC CACCAAGGCA
    TCTGTGGTTG CACAGCCACT GGCAGCTCAG AGCTCTGAGA GCAGCGACTC GTCAGACAGT
    GAGGAGGAGC CTGATGCTAA GAAAACAGCA GCGCCTCTCC AGGGATCCCA GAAAAAGACA
    CTTGCCACAC CTTCCACAGC CACTAGGCCA CCAGGGACAA AAACACCCCT CTTGACTAAA
    CCTGGAAAAC TTGCAACACC AACCAAGACA CCAGTGATTT CTTTGCCGTT AGCAGAAAGT
    AGTGACTCGT CAGAAAGTGA AGACGAGCCA GCAGTCAAGA ATGCACAACT ATCTGTCGTG
    GCGTCCAAGA ACAATAGCAA AACGACTCTC TTGGCCAAGA CAGGGAAGTT GGCTGCTCCA
    GCCAAGACGC CAGCGGTTAC ACAGCCGCCA GTAGCCGAGA GCTCTGAGAG CAGCAACTCA
    TCTGATAGTG AGGAGGAGCT TAACAATAAG CAGAAGACTG CGGCACCTCC CCAGGCATCC
    CAGAAAAAGG GCAAATCGCC TCTCTTGGCC AGTACAGGGA ATGCTGCAAC ACCAGCCAAG
    ACACCAGTGG TTGCACAGCC CCTAACAGCT CAGAGCTCTG AGAGCAGCAA CTCGTCAGAC
    AGTGAGGAGG AGCCTGATGC TAAGAAAATA AATGCATCCT TGAACCCTAC AACATCAGCG
    AGCCAAGCTT TTGCCACTAT GAAGCCAACT GCCAAACAGG ACTGTGACAG TAGTGACACA
    AGTGAAGAAG CCGAGGGCAA ATCCAGGAGT CTGGTGGGGC AACCATCGGT TACCATTATC
    TCGGAGACCA GTGATACCAC TGATATTGAT GAGGTAGTGT CTCCAACTCC TGCTCCAATC
    AAGGCAACCA AAGGAAAGGC TGTACCCATA TTGGCAAAGA CTGTCACACC ATCCAGCCTA
    AAAGCTACCA AAAGCAAAGC CAGCAGTGCA GCTAAAACTG CCAAAGCAGT GCAGCAAATA
    GCTGTAGCCA ACTCTGACAG CAGTGATTCT TTAGATAGCG ATGAGGATCC TGGAGTAAAG
    CAGACACCAT CTGTACCATT AAAGAAAGTA AAGTCAGGGA AAGGAGCTAA ACTGACTGCC
    GTGTGTGTCA GTGGTGGAAA TCAGAAACTG CCCAGTTCGT TACCTGTGCC CTCTTTACAA
    AAGCCCAGTG TATCTGACGT TTCTGAGCCC AGCGACTCGT CCGATAGTGA CATCGATACA
    GGAGCCCAGT CTCTTGTAGC ACCAGTACAT GCGGTGCCCA CCCCACAGAA ACGGAAGAGG
    AATCCAAAAG TCAAAGCTCA AGTGACGCAA CCTCCAGCTG CTTCAGTGAA ACACAAAAAA
    AAGTCTAAAG CAGAGCAACT GGCAGCATCT GCACAGCCAG CACCAGAGCC AGTGATCGAC
    TCTGAAGAGG AGACCATAAT GGCTCTCCTT TCGGGTCAGA GCCCAAAGAA GGGGAAGCAG
    AAACTATCCA AACTGGGACT GAAAGCAGCT CCTTCAGCCA CTCCCCTTCC TGTACAAAAG
    AATGGCAGTG TGTCGGACTC ATCCGATGAC AGCGATATCG GAACTGCAAC ATTAAATATA
    GTTACACCTC CAGCGTTGGA GCTCAGGCCG CCGACCGCTG ACAAAGAAAA GAAAAGTGCA
    AAGAAGAGGA AGAGCTCCTC AGAAGATGGA CCCAAAAAGA AAGCTAAGAA AGCAAAGAAG
    GAAGGAAAGA AAGAAAAGAA GAAATCAAAA GCAGAAGGAG GAGAGGCTGG AGCCAGCACC
    CCTAAAGCAA AGTCCTCCAA AAAAGATAAA AAGAAGCTAG AGAAATCAGA GAAAAAGAAA
    TCAAGCAAAA AAAAAAAAAG GAAAAGAAAA AGAAGGATGG GGAAAAAAAG GAGGCAAAGA
    AGACCAAGAA GGGTTCAGCA ACTGGCGTCT CCCCTAAACC AAAGAAAAAG AAGAAAAGTA
    AAGCGGATAA AGTTGACTGA GGAACACAAG GAAGGAGGGC CTGATATCTA CCCGCCAGGT
    ACAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814129.1
    CDS161..4429
    Translation

    Target ORF information:

    RefSeq Version XM_012958675.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958675.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA CACATACAGT TTTGGCTCAT GTTCATGCAC CACCAAGGAC ACCTCTGCTA
    TCTCCTTCCA AAACTGCAAG GGCTGCTGTG CCAGCAAAAG CTGCTGTTGT CACTAAACCA
    GTAGATATCG ATGAGTCGGA GAGCAGTGAT TCATCGGACA GTGAAATCAT AGTTACCCAG
    GCATCCCAGA AAAAGCCCAG TGATCCTACA GCCGCTAAGC CACTAGTGAC AAAAACACCC
    AACTTGGCCC AACCTGGAAA GGTGGCAACG CCAACAAAGA CACCAGTGAG TGCAAAGCCC
    CTAACAGCTC AGAGCTCTGA GAGCAGCGAC TCGTCAGACA GTGAGGAGGA GCTTAATGCT
    AAGAATACAG CAGCACCTCT CCAGGCATCC CAGAAAAAGA CTATTGTTCC TCCCACATCA
    ACAAGGCCAC CAGGGACAAA AACACCCCTC TTGGCCAAAC CCGGAACATT GACAACACCA
    GCCAAGGCAT CTGTGGTTGC TCAGAGCTCT GAGAGCAGCG ACTCGTCAGA CAGTGAGTTG
    GAGCCTGATG CTAAGAAAAC AGCAGCGCCT CTCCAAGGAT CCCAAAAAAA TATACCTGCC
    ACACCTTCTA CAGCCACTAG GCCACCAGGG ACAAAAACAC CCCTCTTGGC CAAACCTGGG
    AAACTGGCAA CACCAACCAA GGCATCTGTG GTTGCTAAGC CACTGGCAGC TCAAAGCTCT
    GAGAGCAGTG ACTCGTCCGA CAGTGAGGAG GAGCCTGATA CTAAGAAGAC AGCCGTGCCT
    CTCCAGGGAT CTCAGAAAAA AACACCTGTC ACACCTTCCA CAGCCGCTAG ACCACCAGGG
    ACAAAAACAC CCCTCTTGGC CAAACCCGGA ACATTGGCAA CACCAGCCAA AGCATCTGTA
    GTTCCTCAGA GCTCTGAGAG CAGTGACTCG TCAGACAGTG AGTTGGAGCC TGATGCTAAG
    AAAACAGCAG CGCCTCTCCA AGGATCCCAA AAAAATATAC CTGCCACACC TTCTACAGCC
    ACTAGGCCAC CAGGGACAAA AACACCCCTC TTGGCCAAAC CTGGGAAACT GGCAACACCA
    ACCAAGGCAT CTGTGGTTGC TCAGCCACTG GCAGCTCAAA GCTCTGAGAG CAGCGACTCG
    TCCGACAGTG AGGAGGAGCC TGATACTAAG AAGACAGCCG TGCCTCTCCA GGGATCTCAG
    AAAAAAACAC CTGTCACACC TTCCACAGCC GCTAGACCAC CAGGGACAAA AACACCCCTC
    TTGGCCAAAC CCGGAACATT GGCAACACCA GCCAAGGCAT CTGTAGTTGC TCAGAGCTCT
    GAGAGCAGTG ACTCGTCAGA CAGTGAGTTG GAGCCTGATG CTAAGAAAAC AGCAGCTTCT
    CTCCAAGGAT CCCAGAAAAA TACACCAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGGCCAAAC CTGGAAATTT GGCAACACCA GCCAAGGCAT CTATGGTTGC TCAGCCACCG
    GCAGCTCAGA GCTCTGAGAG CAGCGACTTG TCAGACAGTG AGGAGGAACC TAATGCTAAG
    AAAACAGCAG CGCCTCTCCA GGGATCCCAG AAAAAGACAC CTGCCACAGC CGCTAGGCCA
    CCAGAGACAA AAGCACCCCT CTTGGCCAAA CCTGGAAAAC TGGCAACACC CACCAAGGCA
    TCTGTGGTTG CACAGCCACT GGCAGCTCAG AGCTCTGAGA GCAGCGACTC GTCAGACAGT
    GAGGAGGAGC CTGATGCTAA GAAAACAGCA GCGCCTCTCC AGGGATCCCA GAAAAAGACA
    CTTGCCACAC CTTCCACAGC CACTAGGCCA CCAGGGACAA AAACACCCCT CTTGACTAAA
    CCTGGAAAAC TTGCAACACC AACCAAGACA CCAGTGATTT CTTTGCCGTT AGCAGAAAGT
    AGTGACTCGT CAGAAAGTGA AGACGAGCCA GCAGTCAAGA ATGCACAACT ATCTGTCGTG
    GCGTCCAAGA ACAATAGCAA AACGACTCTC TTGGCCAAGA CAGGGAAGTT GGCTGCTCCA
    GCCAAGACGC CAGCGGTTAC ACAGCCGCCA GTAGCCGAGA GCTCTGAGAG CAGCAACTCA
    TCTGATAGTG AGGAGGAGCT TAACAATAAG CAGAAGACTG CGGCACCTCC CCAGGCATCC
    CAGAAAAAGG GCAAATCGCC TCTCTTGGCC AGTACAGGGA ATGCTGCAAC ACCAGCCAAG
    ACACCAGTGG TTGCACAGCC CCTAACAGCT CAGAGCTCTG AGAGCAGCAA CTCGTCAGAC
    AGTGAGGAGG AGCCTGATGC TAAGAAAATA AATGCATCCT TGAACCCTAC AACATCAGCG
    AGCCAAGCTT TTGCCACTAT GAAGCCAACT GCCAAACAGG ACTGTGACAG TAGTGACACA
    AGTGAAGAAG CCGAGGGCAA ATCCAGGAGT CTGGTGGGGC AACCATCGGT TACCATTATC
    TCGGAGACCA GTGATACCAC TGATATTGAT GAGGTAGTGT CTCCAACTCC TGCTCCAATC
    AAGGCAACCA AAGGAAAGGC TGTACCCATA TTGGCAAAGA CTGTCACACC ATCCAGCCTA
    AAAGCTACCA AAAGCAAAGC CAGCAGTGCA GCTAAAACTG CCAAAGCAGT GCAGCAAATA
    GCTGTAGCCA ACTCTGACAG CAGTGATTCT TTAGATAGCG ATGAGGATCC TGGAGTAAAG
    CAGACACCAT CTGTACCATT AAAGAAAGTA AAGTCAGGGA AAGGAGCTAA ACTGACTGCC
    GTGTGTGTCA GTGGTGGAAA TCAGAAACTG CCCAGTTCGT TACCTGTGCC CTCTTTACAA
    AAGCCCAGTG TATCTGACGT TTCTGAGCCC AGCGACTCGT CCGATAGTGA CATCGATACA
    GGAGCCCAGT CTCTTGTAGC ACCAGTACAT GCGGTGCCCA CCCCACAGAA ACGGAAGAGG
    AATCCAAAAG TCAAAGCTCA AGTGACGCAA CCTCCAGCTG CTTCAGTGAA ACACAAAAAA
    AAGTCTAAAG CAGAGCAACT GGCAGCATCT GCACAGCCAG CACCAGAGCC AGTGATCGAC
    TCTGAAGAGG AGACCATAAT GGCTCTCCTT TCGGGTCAGA GCCCAAAGAA GGGGAAGCAG
    AAACTATCCA AACTGGGACT GAAAGCAGCT CCTTCAGCCA CTCCCCTTCC TGTACAAAAG
    AATGGCAGTG TGTCGGACTC ATCCGATGAC AGCGATATCG GAACTGCAAC ATTAAATATA
    GTTACACCTC CAGCGTTGGA GCTCAGGCCG CCGACCGCTG ACAAAGAAAA GAAAAGTGCA
    AAGAAGAGGA AGAGCTCCTC AGAAGATGGA CCCAAAAAGA AAGCTAAGAA AGCAAAGAAG
    GAAGGAAAGA AAGAAAAGAA GAAATCAAAA GCAGAAGGAG GAGAGGCTGG AGCCAGCACC
    CCTAAAGCAA AGTCCTCCAA AAAAGATAAA AAGAAGCTAG AGAAATCAGA GAAAAAGAAA
    TCAAGCAAAA AAAAAAAAAG GAAAAGAAAA AGAAGGATGG GGAAAAAAAG GAGGCAAAGA
    AGACCAAGAA GGGTTCAGCA ACTGGCGTCT CCCCTAAACC AAAGAAAAAG AAGAAAAGTA
    AAGCGGATAA AGTTGACTGA GGAACACAAG GAAGGAGGGC CTGATATCTA CCCGCCAGGT
    ACAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32618
    Clone ID Related Accession (Same CDS sequence) XM_012958677.2
    Accession Version XM_012958677.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958677.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC TTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGC AGAAGACTGC GGCACCTCCC
    CAGGCATCCC AGAAAAAGGG CAAATCGCCT CTCTTGGCCA GTACAGGGAA TGCTGCAACA
    CCAGCCAAGA CACCAGTGGT TGCACAGCCC CTAACAGCTC AGAGCTCTGA GAGCAGCAAC
    TCGTCAGACA GTGAGGAGGA GCCTGATGCT AAGGTAGTGT CTCCAACTCC TGCTCCAATC
    AAGGCAACCA AAGGAAAGGC TGTACCCATA TTGGCAAAGA CTGTCACACC ATCCAGCCTA
    AAAGCTACCA AAAGCAAAGC CAGCAGTGCA GCTAAAACTG CCAAAGCAGT GCAGCAAATA
    GCTGTAGCCA ACTCTGACAG CAGTGATTCT TTAGATAGCG ATGAGGATCC TGGAGTAAAG
    CAGACACCAT CTGTACCATT AAAGAAAGTA AAGTCAGGGA AAGGAGCTAA ACTGACTGCC
    GTGTGTGTCA GTGGTGGAAA TCAGAAACTG CCCAGTTCGT TACCTGTGCC CTCTTTACAA
    AAGCCCAGTG TATCTGACGT TTCTGAGCCC AGCGACTCGT CCGATAGTGA CATCGATACA
    GGAGCCCAGT CTCTTGTAGC ACCAGTACAT GCGGTGCCCA CCCCACAGAA ACGGAAGAGG
    AATCCAAAAG TCAAAGCTCA AGTGACGCAA CCTCCAGCTG CTTCAGTGAA ACACAAAAAA
    AAGTCTAAAG CAGAGCAACT GGCAGCATCT GCACAGCCAG CACCAGAGCC AGTGATCGAC
    TCTGAAGAGG AGACCATAAT GGCTCTCCTT TCGGGTCAGA GCCCAAAGAA GGGGAAGCAG
    AAACTATCCA AACTGGGACT GAAAGCAGCT CCTTCAGCCA CTCCCCTTCC TGTACAAAAG
    AATGGCAGTG TGTCGGACTC ATCCGATGAC AGCGATATCG GAACTGCAAC ATTAAATATA
    GTTACACCTC CAGCGTTGGA GCTCAGGCCG CCGACCGCTG ACAAAGAAAA GAAAAGTGCA
    AAGAAGAGGA AGAGCTCCTC AGAAGATGGA CCCAAAAAGA AAGCTAAGAA AGCAAAGAAG
    GAAGGAAAGA AAGAAAAGAA GAAATCAAAA GCAGAAGGAG GAGAGGCTGG AGCCAGCACC
    CCTAAAGCAA AGTCCTCCAA AAAAGATAAA AAGAAGCTAG AGAAATCAGA GAAAAAGAAA
    TCAAGCAAAA AAAAAAAAAG GAAAAGAAAA AGAAGGATGG GGAAAAAAAG GAGGCAAAGA
    AGACCAAGAA GGGTTCAGCA ACTGGCGTCT CCCCTAAACC AAAGAAAAAG AAGAAAAGTA
    AAGCGGATAA AGTTGACTGA GGAACACAAG GAAGGAGGGC CTGATATCTA CCCGCCAGGT
    ACAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814131.1
    CDS162..4430
    Translation

    Target ORF information:

    RefSeq Version XM_012958677.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958677.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC TTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGC AGAAGACTGC GGCACCTCCC
    CAGGCATCCC AGAAAAAGGG CAAATCGCCT CTCTTGGCCA GTACAGGGAA TGCTGCAACA
    CCAGCCAAGA CACCAGTGGT TGCACAGCCC CTAACAGCTC AGAGCTCTGA GAGCAGCAAC
    TCGTCAGACA GTGAGGAGGA GCCTGATGCT AAGGTAGTGT CTCCAACTCC TGCTCCAATC
    AAGGCAACCA AAGGAAAGGC TGTACCCATA TTGGCAAAGA CTGTCACACC ATCCAGCCTA
    AAAGCTACCA AAAGCAAAGC CAGCAGTGCA GCTAAAACTG CCAAAGCAGT GCAGCAAATA
    GCTGTAGCCA ACTCTGACAG CAGTGATTCT TTAGATAGCG ATGAGGATCC TGGAGTAAAG
    CAGACACCAT CTGTACCATT AAAGAAAGTA AAGTCAGGGA AAGGAGCTAA ACTGACTGCC
    GTGTGTGTCA GTGGTGGAAA TCAGAAACTG CCCAGTTCGT TACCTGTGCC CTCTTTACAA
    AAGCCCAGTG TATCTGACGT TTCTGAGCCC AGCGACTCGT CCGATAGTGA CATCGATACA
    GGAGCCCAGT CTCTTGTAGC ACCAGTACAT GCGGTGCCCA CCCCACAGAA ACGGAAGAGG
    AATCCAAAAG TCAAAGCTCA AGTGACGCAA CCTCCAGCTG CTTCAGTGAA ACACAAAAAA
    AAGTCTAAAG CAGAGCAACT GGCAGCATCT GCACAGCCAG CACCAGAGCC AGTGATCGAC
    TCTGAAGAGG AGACCATAAT GGCTCTCCTT TCGGGTCAGA GCCCAAAGAA GGGGAAGCAG
    AAACTATCCA AACTGGGACT GAAAGCAGCT CCTTCAGCCA CTCCCCTTCC TGTACAAAAG
    AATGGCAGTG TGTCGGACTC ATCCGATGAC AGCGATATCG GAACTGCAAC ATTAAATATA
    GTTACACCTC CAGCGTTGGA GCTCAGGCCG CCGACCGCTG ACAAAGAAAA GAAAAGTGCA
    AAGAAGAGGA AGAGCTCCTC AGAAGATGGA CCCAAAAAGA AAGCTAAGAA AGCAAAGAAG
    GAAGGAAAGA AAGAAAAGAA GAAATCAAAA GCAGAAGGAG GAGAGGCTGG AGCCAGCACC
    CCTAAAGCAA AGTCCTCCAA AAAAGATAAA AAGAAGCTAG AGAAATCAGA GAAAAAGAAA
    TCAAGCAAAA AAAAAAAAAG GAAAAGAAAA AGAAGGATGG GGAAAAAAAG GAGGCAAAGA
    AGACCAAGAA GGGTTCAGCA ACTGGCGTCT CCCCTAAACC AAAGAAAAAG AAGAAAAGTA
    AAGCGGATAA AGTTGACTGA GGAACACAAG GAAGGAGGGC CTGATATCTA CCCGCCAGGT
    ACAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32619
    Clone ID Related Accession (Same CDS sequence) XM_012958678.2
    Accession Version XM_012958678.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4263bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958678.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCGCCTC TCCAGGGATC CCAGAAAAAG ACACCTGCCA CAGCCGCTAG GCCACCAGAG
    ACAAAAGCAC CCCTCTTGGC CAAACCTGGA AAACTGGCAA CACCCACCAA GGCATCTGTG
    GTTGCACAGC CACTGGCAGC TCAGAGCTCT GAGAGCAGCG ACTCGTCAGA CAGTGAGGAG
    GAGCCTGATG CTAAGAAAAC AGCAGCGCCT CTCCAGGGAT CCCAGAAAAA GACACTTGCC
    ACACCTTCCA CAGCCACTAG GCCACCAGGG ACAAAAACAC CCCTCTTGAC TAAACCTGGA
    AAACTTGCAA CACCAACCAA GACACCAGTG ATTTCTTTGC CGTTAGCAGA AAGTAGTGAC
    TCGTCAGAAA GTGAAGACGA GCCAGCAGTC AAGAATGCAC AACTATCTGT CGTGGCGTCC
    AAGAACAATA GCAAAACGAC TCTCTTGGCC AAGACAGGGA AGTTGGCTGC TCCAGCCAAG
    ACGCCAGCGG TTACACAGCC GCCAGTAGCC GAGAGCTCTG AGAGCAGCAA CTCATCTGAT
    AGTGAGGAGG AGCTTAACAA TAAGCAGAAG ACTGCGGCAC CTCCCCAGGC ATCCCAGAAA
    AAGGGCAAAT CGCCTCTCTT GGCCAGTACA GGGAATGCTG CAACACCAGC CAAGACACCA
    GTGGTTGCAC AGCCCCTAAC AGCTCAGAGC TCTGAGAGCA GCAACTCGTC AGACAGTGAG
    GAGGAGCCTG ATGCTAAGAA AATAAATGCA TCCTTGAACC CTACAACATC AGCGAGCCAA
    GCTTTTGCCA CTATGAAGCC AACTGCCAAA CAGGACTGTG ACAGTAGTGA CACAAGTGAA
    GAAGCCGAGG GCAAATCCAG GAGTCTGGTG GGGCAACCAT CGGTTACCAT TATCTCGGAG
    ACCAGTGATA CCACTGATAT TGATGAGGTA GTGTCTCCAA CTCCTGCTCC AATCAAGGCA
    ACCAAAGGAA AGGCTGTACC CATATTGGCA AAGACTGTCA CACCATCCAG CCTAAAAGCT
    ACCAAAAGCA AAGCCAGCAG TGCAGCTAAA ACTGCCAAAG CAGTGCAGCA AATAGCTGTA
    GCCAACTCTG ACAGCAGTGA TTCTTTAGAT AGCGATGAGG ATCCTGGAGT AAAGCAGACA
    CCATCTGTAC CATTAAAGAA AGTAAAGTCA GGGAAAGGAG CTAAACTGAC TGCCGTGTGT
    GTCAGTGGTG GAAATCAGAA ACTGCCCAGT TCGTTACCTG TGCCCTCTTT ACAAAAGCCC
    AGTGTATCTG ACGTTTCTGA GCCCAGCGAC TCGTCCGATA GTGACATCGA TACAGGAGCC
    CAGTCTCTTG TAGCACCAGT ACATGCGGTG CCCACCCCAC AGAAACGGAA GAGGAATCCA
    AAAGTCAAAG CTCAAGTGAC GCAACCTCCA GCTGCTTCAG TGAAACACAA AAAAAAGTCT
    AAAGCAGAGC AACTGGCAGC ATCTGCACAG CCAGCACCAG AGCCAGTGAT CGACTCTGAA
    GAGGAGACCA TAATGGCTCT CCTTTCGGGT CAGAGCCCAA AGAAGGGGAA GCAGAAACTA
    TCCAAACTGG GACTGAAAGC AGCTCCTTCA GCCACTCCCC TTCCTGTACA AAAGAATGGC
    AGTGTGTCGG ACTCATCCGA TGACAGCGAT ATCGGAACTG CAACATTAAA TATAGTTACA
    CCTCCAGCGT TGGAGCTCAG GCCGCCGACC GCTGACAAAG AAAAGAAAAG TGCAAAGAAG
    AGGAAGAGCT CCTCAGAAGA TGGACCCAAA AAGAAAGCTA AGAAAGCAAA GAAGGAAGGA
    AAGAAAGAAA AGAAGAAATC AAAAGCAGAA GGAGGAGAGG CTGGAGCCAG CACCCCTAAA
    GCAAAGTCCT CCAAAAAAGA TAAAAAGAAG CTAGAGAAAT CAGAGAAAAA GAAATCAAGC
    AAAAAAAAAA AAAGGAAAAG AAAAAGAAGG ATGGGGAAAA AAAGGAGGCA AAGAAGACCA
    AGAAGGGTTC AGCAACTGGC GTCTCCCCTA AACCAAAGAA AAAGAAGAAA AGTAAAGCGG
    ATAAAGTTGA CTGAGGAACA CAAGGAAGGA GGGCCTGATA TCTACCCGCC AGGTACAGAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814132.1
    CDS162..4424
    Translation

    Target ORF information:

    RefSeq Version XM_012958678.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958678.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCGCCTC TCCAGGGATC CCAGAAAAAG ACACCTGCCA CAGCCGCTAG GCCACCAGAG
    ACAAAAGCAC CCCTCTTGGC CAAACCTGGA AAACTGGCAA CACCCACCAA GGCATCTGTG
    GTTGCACAGC CACTGGCAGC TCAGAGCTCT GAGAGCAGCG ACTCGTCAGA CAGTGAGGAG
    GAGCCTGATG CTAAGAAAAC AGCAGCGCCT CTCCAGGGAT CCCAGAAAAA GACACTTGCC
    ACACCTTCCA CAGCCACTAG GCCACCAGGG ACAAAAACAC CCCTCTTGAC TAAACCTGGA
    AAACTTGCAA CACCAACCAA GACACCAGTG ATTTCTTTGC CGTTAGCAGA AAGTAGTGAC
    TCGTCAGAAA GTGAAGACGA GCCAGCAGTC AAGAATGCAC AACTATCTGT CGTGGCGTCC
    AAGAACAATA GCAAAACGAC TCTCTTGGCC AAGACAGGGA AGTTGGCTGC TCCAGCCAAG
    ACGCCAGCGG TTACACAGCC GCCAGTAGCC GAGAGCTCTG AGAGCAGCAA CTCATCTGAT
    AGTGAGGAGG AGCTTAACAA TAAGCAGAAG ACTGCGGCAC CTCCCCAGGC ATCCCAGAAA
    AAGGGCAAAT CGCCTCTCTT GGCCAGTACA GGGAATGCTG CAACACCAGC CAAGACACCA
    GTGGTTGCAC AGCCCCTAAC AGCTCAGAGC TCTGAGAGCA GCAACTCGTC AGACAGTGAG
    GAGGAGCCTG ATGCTAAGAA AATAAATGCA TCCTTGAACC CTACAACATC AGCGAGCCAA
    GCTTTTGCCA CTATGAAGCC AACTGCCAAA CAGGACTGTG ACAGTAGTGA CACAAGTGAA
    GAAGCCGAGG GCAAATCCAG GAGTCTGGTG GGGCAACCAT CGGTTACCAT TATCTCGGAG
    ACCAGTGATA CCACTGATAT TGATGAGGTA GTGTCTCCAA CTCCTGCTCC AATCAAGGCA
    ACCAAAGGAA AGGCTGTACC CATATTGGCA AAGACTGTCA CACCATCCAG CCTAAAAGCT
    ACCAAAAGCA AAGCCAGCAG TGCAGCTAAA ACTGCCAAAG CAGTGCAGCA AATAGCTGTA
    GCCAACTCTG ACAGCAGTGA TTCTTTAGAT AGCGATGAGG ATCCTGGAGT AAAGCAGACA
    CCATCTGTAC CATTAAAGAA AGTAAAGTCA GGGAAAGGAG CTAAACTGAC TGCCGTGTGT
    GTCAGTGGTG GAAATCAGAA ACTGCCCAGT TCGTTACCTG TGCCCTCTTT ACAAAAGCCC
    AGTGTATCTG ACGTTTCTGA GCCCAGCGAC TCGTCCGATA GTGACATCGA TACAGGAGCC
    CAGTCTCTTG TAGCACCAGT ACATGCGGTG CCCACCCCAC AGAAACGGAA GAGGAATCCA
    AAAGTCAAAG CTCAAGTGAC GCAACCTCCA GCTGCTTCAG TGAAACACAA AAAAAAGTCT
    AAAGCAGAGC AACTGGCAGC ATCTGCACAG CCAGCACCAG AGCCAGTGAT CGACTCTGAA
    GAGGAGACCA TAATGGCTCT CCTTTCGGGT CAGAGCCCAA AGAAGGGGAA GCAGAAACTA
    TCCAAACTGG GACTGAAAGC AGCTCCTTCA GCCACTCCCC TTCCTGTACA AAAGAATGGC
    AGTGTGTCGG ACTCATCCGA TGACAGCGAT ATCGGAACTG CAACATTAAA TATAGTTACA
    CCTCCAGCGT TGGAGCTCAG GCCGCCGACC GCTGACAAAG AAAAGAAAAG TGCAAAGAAG
    AGGAAGAGCT CCTCAGAAGA TGGACCCAAA AAGAAAGCTA AGAAAGCAAA GAAGGAAGGA
    AAGAAAGAAA AGAAGAAATC AAAAGCAGAA GGAGGAGAGG CTGGAGCCAG CACCCCTAAA
    GCAAAGTCCT CCAAAAAAGA TAAAAAGAAG CTAGAGAAAT CAGAGAAAAA GAAATCAAGC
    AAAAAAAAAA AAAGGAAAAG AAAAAGAAGG ATGGGGAAAA AAAGGAGGCA AAGAAGACCA
    AGAAGGGTTC AGCAACTGGC GTCTCCCCTA AACCAAAGAA AAAGAAGAAA AGTAAAGCGG
    ATAAAGTTGA CTGAGGAACA CAAGGAAGGA GGGCCTGATA TCTACCCGCC AGGTACAGAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32620
    Clone ID Related Accession (Same CDS sequence) XM_012958679.2
    Accession Version XM_012958679.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4257bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958679.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACT TGCCACACCT
    TCCACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGACTAAACC TGGAAAACTT
    GCAACACCAA CCAAGACACC AGTGATTTCT TTGCCGTTAG CAGAAAGTAG TGACTCGTCA
    GAAAGTGAAG ACGAGCCAGC AGTCAAGAAT GCACAACTAT CTGTCGTGGC GTCCAAGAAC
    AATAGCAAAA CGACTCTCTT GGCCAAGACA GGGAAGTTGG CTGCTCCAGC CAAGACGCCA
    GCGGTTACAC AGCCGCCAGT AGCCGAGAGC TCTGAGAGCA GCAACTCATC TGATAGTGAG
    GAGGAGCTTA ACAATAAGCA GAAGACTGCG GCACCTCCCC AGGCATCCCA GAAAAAGGGC
    AAATCGCCTC TCTTGGCCAG TACAGGGAAT GCTGCAACAC CAGCCAAGAC ACCAGTGGTT
    GCACAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCAACT CGTCAGACAG TGAGGAGGAG
    CCTGATGCTA AGAAAATAAA TGCATCCTTG AACCCTACAA CATCAGCGAG CCAAGCTTTT
    GCCACTATGA AGCCAACTGC CAAACAGGAC TGTGACAGTA GTGACACAAG TGAAGAAGCC
    GAGGGCAAAT CCAGGAGTCT GGTGGGGCAA CCATCGGTTA CCATTATCTC GGAGACCAGT
    GATACCACTG ATATTGATGA GGTAGTGTCT CCAACTCCTG CTCCAATCAA GGCAACCAAA
    GGAAAGGCTG TACCCATATT GGCAAAGACT GTCACACCAT CCAGCCTAAA AGCTACCAAA
    AGCAAAGCCA GCAGTGCAGC TAAAACTGCC AAAGCAGTGC AGCAAATAGC TGTAGCCAAC
    TCTGACAGCA GTGATTCTTT AGATAGCGAT GAGGATCCTG GAGTAAAGCA GACACCATCT
    GTACCATTAA AGAAAGTAAA GTCAGGGAAA GGAGCTAAAC TGACTGCCGT GTGTGTCAGT
    GGTGGAAATC AGAAACTGCC CAGTTCGTTA CCTGTGCCCT CTTTACAAAA GCCCAGTGTA
    TCTGACGTTT CTGAGCCCAG CGACTCGTCC GATAGTGACA TCGATACAGG AGCCCAGTCT
    CTTGTAGCAC CAGTACATGC GGTGCCCACC CCACAGAAAC GGAAGAGGAA TCCAAAAGTC
    AAAGCTCAAG TGACGCAACC TCCAGCTGCT TCAGTGAAAC ACAAAAAAAA GTCTAAAGCA
    GAGCAACTGG CAGCATCTGC ACAGCCAGCA CCAGAGCCAG TGATCGACTC TGAAGAGGAG
    ACCATAATGG CTCTCCTTTC GGGTCAGAGC CCAAAGAAGG GGAAGCAGAA ACTATCCAAA
    CTGGGACTGA AAGCAGCTCC TTCAGCCACT CCCCTTCCTG TACAAAAGAA TGGCAGTGTG
    TCGGACTCAT CCGATGACAG CGATATCGGA ACTGCAACAT TAAATATAGT TACACCTCCA
    GCGTTGGAGC TCAGGCCGCC GACCGCTGAC AAAGAAAAGA AAAGTGCAAA GAAGAGGAAG
    AGCTCCTCAG AAGATGGACC CAAAAAGAAA GCTAAGAAAG CAAAGAAGGA AGGAAAGAAA
    GAAAAGAAGA AATCAAAAGC AGAAGGAGGA GAGGCTGGAG CCAGCACCCC TAAAGCAAAG
    TCCTCCAAAA AAGATAAAAA GAAGCTAGAG AAATCAGAGA AAAAGAAATC AAGCAAAAAA
    AAAAAAAGGA AAAGAAAAAG AAGGATGGGG AAAAAAAGGA GGCAAAGAAG ACCAAGAAGG
    GTTCAGCAAC TGGCGTCTCC CCTAAACCAA AGAAAAAGAA GAAAAGTAAA GCGGATAAAG
    TTGACTGAGG AACACAAGGA AGGAGGGCCT GATATCTACC CGCCAGGTAC AGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814133.1
    CDS162..4418
    Translation

    Target ORF information:

    RefSeq Version XM_012958679.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958679.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACT TGCCACACCT
    TCCACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGACTAAACC TGGAAAACTT
    GCAACACCAA CCAAGACACC AGTGATTTCT TTGCCGTTAG CAGAAAGTAG TGACTCGTCA
    GAAAGTGAAG ACGAGCCAGC AGTCAAGAAT GCACAACTAT CTGTCGTGGC GTCCAAGAAC
    AATAGCAAAA CGACTCTCTT GGCCAAGACA GGGAAGTTGG CTGCTCCAGC CAAGACGCCA
    GCGGTTACAC AGCCGCCAGT AGCCGAGAGC TCTGAGAGCA GCAACTCATC TGATAGTGAG
    GAGGAGCTTA ACAATAAGCA GAAGACTGCG GCACCTCCCC AGGCATCCCA GAAAAAGGGC
    AAATCGCCTC TCTTGGCCAG TACAGGGAAT GCTGCAACAC CAGCCAAGAC ACCAGTGGTT
    GCACAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCAACT CGTCAGACAG TGAGGAGGAG
    CCTGATGCTA AGAAAATAAA TGCATCCTTG AACCCTACAA CATCAGCGAG CCAAGCTTTT
    GCCACTATGA AGCCAACTGC CAAACAGGAC TGTGACAGTA GTGACACAAG TGAAGAAGCC
    GAGGGCAAAT CCAGGAGTCT GGTGGGGCAA CCATCGGTTA CCATTATCTC GGAGACCAGT
    GATACCACTG ATATTGATGA GGTAGTGTCT CCAACTCCTG CTCCAATCAA GGCAACCAAA
    GGAAAGGCTG TACCCATATT GGCAAAGACT GTCACACCAT CCAGCCTAAA AGCTACCAAA
    AGCAAAGCCA GCAGTGCAGC TAAAACTGCC AAAGCAGTGC AGCAAATAGC TGTAGCCAAC
    TCTGACAGCA GTGATTCTTT AGATAGCGAT GAGGATCCTG GAGTAAAGCA GACACCATCT
    GTACCATTAA AGAAAGTAAA GTCAGGGAAA GGAGCTAAAC TGACTGCCGT GTGTGTCAGT
    GGTGGAAATC AGAAACTGCC CAGTTCGTTA CCTGTGCCCT CTTTACAAAA GCCCAGTGTA
    TCTGACGTTT CTGAGCCCAG CGACTCGTCC GATAGTGACA TCGATACAGG AGCCCAGTCT
    CTTGTAGCAC CAGTACATGC GGTGCCCACC CCACAGAAAC GGAAGAGGAA TCCAAAAGTC
    AAAGCTCAAG TGACGCAACC TCCAGCTGCT TCAGTGAAAC ACAAAAAAAA GTCTAAAGCA
    GAGCAACTGG CAGCATCTGC ACAGCCAGCA CCAGAGCCAG TGATCGACTC TGAAGAGGAG
    ACCATAATGG CTCTCCTTTC GGGTCAGAGC CCAAAGAAGG GGAAGCAGAA ACTATCCAAA
    CTGGGACTGA AAGCAGCTCC TTCAGCCACT CCCCTTCCTG TACAAAAGAA TGGCAGTGTG
    TCGGACTCAT CCGATGACAG CGATATCGGA ACTGCAACAT TAAATATAGT TACACCTCCA
    GCGTTGGAGC TCAGGCCGCC GACCGCTGAC AAAGAAAAGA AAAGTGCAAA GAAGAGGAAG
    AGCTCCTCAG AAGATGGACC CAAAAAGAAA GCTAAGAAAG CAAAGAAGGA AGGAAAGAAA
    GAAAAGAAGA AATCAAAAGC AGAAGGAGGA GAGGCTGGAG CCAGCACCCC TAAAGCAAAG
    TCCTCCAAAA AAGATAAAAA GAAGCTAGAG AAATCAGAGA AAAAGAAATC AAGCAAAAAA
    AAAAAAAGGA AAAGAAAAAG AAGGATGGGG AAAAAAAGGA GGCAAAGAAG ACCAAGAAGG
    GTTCAGCAAC TGGCGTCTCC CCTAAACCAA AGAAAAAGAA GAAAAGTAAA GCGGATAAAG
    TTGACTGAGG AACACAAGGA AGGAGGGCCT GATATCTACC CGCCAGGTAC AGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32621
    Clone ID Related Accession (Same CDS sequence) XM_012958680.2
    Accession Version XM_012958680.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3915bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958680.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAATAAATG CATCCTTGAA CCCTACAACA
    TCAGCGAGCC AAGCTTTTGC CACTATGAAG CCAACTGCCA AACAGGACTG TGACAGTAGT
    GACACAAGTG AAGAAGCCGA GGGCAAATCC AGGAGTCTGG TGGGGCAACC ATCGGTTACC
    ATTATCTCGG AGACCAGTGA TACCACTGAT ATTGATGAGG TAGTGTCTCC AACTCCTGCT
    CCAATCAAGG CAACCAAAGG AAAGGCTGTA CCCATATTGG CAAAGACTGT CACACCATCC
    AGCCTAAAAG CTACCAAAAG CAAAGCCAGC AGTGCAGCTA AAACTGCCAA AGCAGTGCAG
    CAAATAGCTG TAGCCAACTC TGACAGCAGT GATTCTTTAG ATAGCGATGA GGATCCTGGA
    GTAAAGCAGA CACCATCTGT ACCATTAAAG AAAGTAAAGT CAGGGAAAGG AGCTAAACTG
    ACTGCCGTGT GTGTCAGTGG TGGAAATCAG AAACTGCCCA GTTCGTTACC TGTGCCCTCT
    TTACAAAAGC CCAGTGTATC TGACGTTTCT GAGCCCAGCG ACTCGTCCGA TAGTGACATC
    GATACAGGAG CCCAGTCTCT TGTAGCACCA GTACATGCGG TGCCCACCCC ACAGAAACGG
    AAGAGGAATC CAAAAGTCAA AGCTCAAGTG ACGCAACCTC CAGCTGCTTC AGTGAAACAC
    AAAAAAAAGT CTAAAGCAGA GCAACTGGCA GCATCTGCAC AGCCAGCACC AGAGCCAGTG
    ATCGACTCTG AAGAGGAGAC CATAATGGCT CTCCTTTCGG GTCAGAGCCC AAAGAAGGGG
    AAGCAGAAAC TATCCAAACT GGGACTGAAA GCAGCTCCTT CAGCCACTCC CCTTCCTGTA
    CAAAAGAATG GCAGTGTGTC GGACTCATCC GATGACAGCG ATATCGGAAC TGCAACATTA
    AATATAGTTA CACCTCCAGC GTTGGAGCTC AGGCCGCCGA CCGCTGACAA AGAAAAGAAA
    AGTGCAAAGA AGAGGAAGAG CTCCTCAGAA GATGGACCCA AAAAGAAAGC TAAGAAAGCA
    AAGAAGGAAG GAAAGAAAGA AAAGAAGAAA TCAAAAGCAG AAGGAGGAGA GGCTGGAGCC
    AGCACCCCTA AAGCAAAGTC CTCCAAAAAA GATAAAAAGA AGCTAGAGAA ATCAGAGAAA
    AAGAAATCAA GCAAAAAAAA AAAAAGGAAA AGAAAAAGAA GGATGGGGAA AAAAAGGAGG
    CAAAGAAGAC CAAGAAGGGT TCAGCAACTG GCGTCTCCCC TAAACCAAAG AAAAAGAAGA
    AAAGTAAAGC GGATAAAGTT GACTGAGGAA CACAAGGAAG GAGGGCCTGA TATCTACCCG
    CCAGGTACAG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814134.1
    CDS162..4076
    Translation

    Target ORF information:

    RefSeq Version XM_012958680.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958680.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAATAAATG CATCCTTGAA CCCTACAACA
    TCAGCGAGCC AAGCTTTTGC CACTATGAAG CCAACTGCCA AACAGGACTG TGACAGTAGT
    GACACAAGTG AAGAAGCCGA GGGCAAATCC AGGAGTCTGG TGGGGCAACC ATCGGTTACC
    ATTATCTCGG AGACCAGTGA TACCACTGAT ATTGATGAGG TAGTGTCTCC AACTCCTGCT
    CCAATCAAGG CAACCAAAGG AAAGGCTGTA CCCATATTGG CAAAGACTGT CACACCATCC
    AGCCTAAAAG CTACCAAAAG CAAAGCCAGC AGTGCAGCTA AAACTGCCAA AGCAGTGCAG
    CAAATAGCTG TAGCCAACTC TGACAGCAGT GATTCTTTAG ATAGCGATGA GGATCCTGGA
    GTAAAGCAGA CACCATCTGT ACCATTAAAG AAAGTAAAGT CAGGGAAAGG AGCTAAACTG
    ACTGCCGTGT GTGTCAGTGG TGGAAATCAG AAACTGCCCA GTTCGTTACC TGTGCCCTCT
    TTACAAAAGC CCAGTGTATC TGACGTTTCT GAGCCCAGCG ACTCGTCCGA TAGTGACATC
    GATACAGGAG CCCAGTCTCT TGTAGCACCA GTACATGCGG TGCCCACCCC ACAGAAACGG
    AAGAGGAATC CAAAAGTCAA AGCTCAAGTG ACGCAACCTC CAGCTGCTTC AGTGAAACAC
    AAAAAAAAGT CTAAAGCAGA GCAACTGGCA GCATCTGCAC AGCCAGCACC AGAGCCAGTG
    ATCGACTCTG AAGAGGAGAC CATAATGGCT CTCCTTTCGG GTCAGAGCCC AAAGAAGGGG
    AAGCAGAAAC TATCCAAACT GGGACTGAAA GCAGCTCCTT CAGCCACTCC CCTTCCTGTA
    CAAAAGAATG GCAGTGTGTC GGACTCATCC GATGACAGCG ATATCGGAAC TGCAACATTA
    AATATAGTTA CACCTCCAGC GTTGGAGCTC AGGCCGCCGA CCGCTGACAA AGAAAAGAAA
    AGTGCAAAGA AGAGGAAGAG CTCCTCAGAA GATGGACCCA AAAAGAAAGC TAAGAAAGCA
    AAGAAGGAAG GAAAGAAAGA AAAGAAGAAA TCAAAAGCAG AAGGAGGAGA GGCTGGAGCC
    AGCACCCCTA AAGCAAAGTC CTCCAAAAAA GATAAAAAGA AGCTAGAGAA ATCAGAGAAA
    AAGAAATCAA GCAAAAAAAA AAAAAGGAAA AGAAAAAGAA GGATGGGGAA AAAAAGGAGG
    CAAAGAAGAC CAAGAAGGGT TCAGCAACTG GCGTCTCCCC TAAACCAAAG AAAAAGAAGA
    AAAGTAAAGC GGATAAAGTT GACTGAGGAA CACAAGGAAG GAGGGCCTGA TATCTACCCG
    CCAGGTACAG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32622
    Clone ID Related Accession (Same CDS sequence) XM_012958681.2
    Accession Version XM_012958681.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958681.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCTTCTC TCCAAGGATC CCAGAAAAAT
    ACACCAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGAAATTTG
    GCAACACCAG CCAAGGCATC TATGGTTGCT CAGCCACCGG CAGCTCAGAG CTCTGAGAGC
    AGCGACTTGT CAGACAGTGA GGAGGAACCT AATGCTAAGA AAACAGCAGC GCCTCTCCAG
    GGATCCCAGA AAAAGACACC TGCCACAGCC GCTAGGCCAC CAGAGACAAA AGCACCCCTC
    TTGGCCAAAC CTGGAAAACT GGCAACACCC ACCAAGGCAT CTGTGGTTGC ACAGCCACTG
    GCAGCTCAGA GCTCTGAGAG CAGCGACTCG TCAGACAGTG AGGAGGAGCC TGATGCTAAG
    AAAACAGCAG CGCCTCTCCA GGGATCCCAG AAAAAGACAC TTGCCACACC TTCCACAGCC
    ACTAGGCCAC CAGGGACAAA AACACCCCTC TTGACTAAAC CTGGAAAACT TGCAACACCA
    ACCAAGACAC CAGTGATTTC TTTGCCGTTA GCAGAAAGTA GTGACTCGTC AGAAAGTGAA
    GACGAGCCAG CAGTCAAGAA TGCACAACTA TCTGTCGTGG CGTCCAAGAA CAATAGCAAA
    ACGACTCTCT TGGCCAAGAC AGGGAAGTTG GCTGCTCCAG CCAAGACGCC AGCGGTTACA
    CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC AGCAACTCAT CTGATAGTGA GGAGGAGCTT
    AACAATAAGC AGAAGACTGC GGCACCTCCC CAGGCATCCC AGAAAAAGGG CAAATCGCCT
    CTCTTGGCCA GTACAGGGAA TGCTGCAACA CCAGCCAAGA CACCAGTGGT TGCACAGCCC
    CTAACAGCTC AGAGCTCTGA GAGCAGCAAC TCGTCAGACA GTGAGGAGGA GCCTGATGCT
    AAGAAAATAA ATGCATCCTT GAACCCTACA ACATCAGCGA GCCAAGCTTT TGCCACTATG
    AAGCCAACTG CCAAACAGGA CTGTGACAGT AGTGACACAA GTGAAGAAGC CGAGGGCAAA
    TCCAGGAGTC TGGTGGGGCA ACCATCGGTT ACCATTATCT CGGAGACCAG TGATACCACT
    GATATTGATG AGGTAGTGTC TCCAACTCCT GCTCCAATCA AGGCAACCAA AGGAAAGGCT
    GTACCCATAT TGGCAAAGAC TGTCACACCA TCCAGCCTAA AAGCTACCAA AAGCAAAGCC
    AGCAGTGCAG CTAAAACTGC CAAAGCAGTG CAGCAAATAG CTGTAGCCAA CTCTGACAGC
    AGTGATTCTT TAGATAGCGA TGAGGATCCT GGAGTAAAGC AGACACCATC TGTACCATTA
    AAGAAAGTAA AGTCAGGGAA AGGAGCTAAA CTGACTGCCG TGTGTGTCAG TGGTGGAAAT
    CAGAAACTGC CCAGTTCGTT ACCTGTGCCC TCTTTACAAA AGCCCAGTGT ATCTGACGTT
    TCTGAGCCCA GCGACTCGTC CGATAGTGAC ATCGATACAG GAGCCCAGTC TCTTGTAGCA
    CCAGTACATG CGGTGCCCAC CCCACAGAAA CGGAAGAGGA ATCCAAAAGT CAAAGCTCAA
    GTGACGCAAC CTCCAGCTGC TTCAGTGAAA CACAAAAAAA AGTCTAAAGC AGAGCAACTG
    GCAGCATCTG CACAGCCAGC ACCAGAGCCA GTGATCGACT CTGAAGAGGA GACCATAATG
    GCTCTCCTTT CGGGTCAGAG CCCAAAGAAG GGGAAGCAGA AACTATCCAA ACTGGGACTG
    AAAGCAGCTC CTTCAGCCAC TCCCCTTCCT GTACAAAAGA ATGGCAGTGT GTCGGACTCA
    TCCGATGACA GCGATATCGG AACTGCAACA TTAAATATAG TTACACCTCC AGCGTTGGAG
    CTCAGGCCGC CGACCGCTGA CAAAGAAAAG AAAAGTGCAA AGAAGAGGAA GAGCTCCTCA
    GAAGATGGAC CCAAAAAGAA AGCTAAGAAA GCAAAGAAGG AAGGAAAGAA AGAAAAGAAG
    AAATCAAAAG CAGAAGGAGG AGAGGCTGGA GCCAGCACCC CTAAAGCAAA GTCCTCCAAA
    AAAGATAAAA AGAAGCTAGA GAAATCAGAG AAAAAGAAAT CAAGCAAAAA AAAAAAAAGG
    AAAAGAAAAA GAAGGATGGG GAAAAAAAGG AGGCAAAGAA GACCAAGAAG GGTTCAGCAA
    CTGGCGTCTC CCCTAAACCA AAGAAAAAGA AGAAAAGTAA AGCGGATAAA GTTGACTGAG
    GAACACAAGG AAGGAGGGCC TGATATCTAC CCGCCAGGTA CAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814135.1
    CDS162..3809
    Translation

    Target ORF information:

    RefSeq Version XM_012958681.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958681.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCTTCTC TCCAAGGATC CCAGAAAAAT
    ACACCAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGAAATTTG
    GCAACACCAG CCAAGGCATC TATGGTTGCT CAGCCACCGG CAGCTCAGAG CTCTGAGAGC
    AGCGACTTGT CAGACAGTGA GGAGGAACCT AATGCTAAGA AAACAGCAGC GCCTCTCCAG
    GGATCCCAGA AAAAGACACC TGCCACAGCC GCTAGGCCAC CAGAGACAAA AGCACCCCTC
    TTGGCCAAAC CTGGAAAACT GGCAACACCC ACCAAGGCAT CTGTGGTTGC ACAGCCACTG
    GCAGCTCAGA GCTCTGAGAG CAGCGACTCG TCAGACAGTG AGGAGGAGCC TGATGCTAAG
    AAAACAGCAG CGCCTCTCCA GGGATCCCAG AAAAAGACAC TTGCCACACC TTCCACAGCC
    ACTAGGCCAC CAGGGACAAA AACACCCCTC TTGACTAAAC CTGGAAAACT TGCAACACCA
    ACCAAGACAC CAGTGATTTC TTTGCCGTTA GCAGAAAGTA GTGACTCGTC AGAAAGTGAA
    GACGAGCCAG CAGTCAAGAA TGCACAACTA TCTGTCGTGG CGTCCAAGAA CAATAGCAAA
    ACGACTCTCT TGGCCAAGAC AGGGAAGTTG GCTGCTCCAG CCAAGACGCC AGCGGTTACA
    CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC AGCAACTCAT CTGATAGTGA GGAGGAGCTT
    AACAATAAGC AGAAGACTGC GGCACCTCCC CAGGCATCCC AGAAAAAGGG CAAATCGCCT
    CTCTTGGCCA GTACAGGGAA TGCTGCAACA CCAGCCAAGA CACCAGTGGT TGCACAGCCC
    CTAACAGCTC AGAGCTCTGA GAGCAGCAAC TCGTCAGACA GTGAGGAGGA GCCTGATGCT
    AAGAAAATAA ATGCATCCTT GAACCCTACA ACATCAGCGA GCCAAGCTTT TGCCACTATG
    AAGCCAACTG CCAAACAGGA CTGTGACAGT AGTGACACAA GTGAAGAAGC CGAGGGCAAA
    TCCAGGAGTC TGGTGGGGCA ACCATCGGTT ACCATTATCT CGGAGACCAG TGATACCACT
    GATATTGATG AGGTAGTGTC TCCAACTCCT GCTCCAATCA AGGCAACCAA AGGAAAGGCT
    GTACCCATAT TGGCAAAGAC TGTCACACCA TCCAGCCTAA AAGCTACCAA AAGCAAAGCC
    AGCAGTGCAG CTAAAACTGC CAAAGCAGTG CAGCAAATAG CTGTAGCCAA CTCTGACAGC
    AGTGATTCTT TAGATAGCGA TGAGGATCCT GGAGTAAAGC AGACACCATC TGTACCATTA
    AAGAAAGTAA AGTCAGGGAA AGGAGCTAAA CTGACTGCCG TGTGTGTCAG TGGTGGAAAT
    CAGAAACTGC CCAGTTCGTT ACCTGTGCCC TCTTTACAAA AGCCCAGTGT ATCTGACGTT
    TCTGAGCCCA GCGACTCGTC CGATAGTGAC ATCGATACAG GAGCCCAGTC TCTTGTAGCA
    CCAGTACATG CGGTGCCCAC CCCACAGAAA CGGAAGAGGA ATCCAAAAGT CAAAGCTCAA
    GTGACGCAAC CTCCAGCTGC TTCAGTGAAA CACAAAAAAA AGTCTAAAGC AGAGCAACTG
    GCAGCATCTG CACAGCCAGC ACCAGAGCCA GTGATCGACT CTGAAGAGGA GACCATAATG
    GCTCTCCTTT CGGGTCAGAG CCCAAAGAAG GGGAAGCAGA AACTATCCAA ACTGGGACTG
    AAAGCAGCTC CTTCAGCCAC TCCCCTTCCT GTACAAAAGA ATGGCAGTGT GTCGGACTCA
    TCCGATGACA GCGATATCGG AACTGCAACA TTAAATATAG TTACACCTCC AGCGTTGGAG
    CTCAGGCCGC CGACCGCTGA CAAAGAAAAG AAAAGTGCAA AGAAGAGGAA GAGCTCCTCA
    GAAGATGGAC CCAAAAAGAA AGCTAAGAAA GCAAAGAAGG AAGGAAAGAA AGAAAAGAAG
    AAATCAAAAG CAGAAGGAGG AGAGGCTGGA GCCAGCACCC CTAAAGCAAA GTCCTCCAAA
    AAAGATAAAA AGAAGCTAGA GAAATCAGAG AAAAAGAAAT CAAGCAAAAA AAAAAAAAGG
    AAAAGAAAAA GAAGGATGGG GAAAAAAAGG AGGCAAAGAA GACCAAGAAG GGTTCAGCAA
    CTGGCGTCTC CCCTAAACCA AAGAAAAAGA AGAAAAGTAA AGCGGATAAA GTTGACTGAG
    GAACACAAGG AAGGAGGGCC TGATATCTAC CCGCCAGGTA CAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43053
    Clone ID Related Accession (Same CDS sequence) XM_018091967.1
    Accession Version XM_018091967.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC TTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGA AGACTGCGGC ACCTCCCCAG
    GCATCCCAGA AAAAGGGCAA ATCGCCTCTC TTGGCCAGTA CAGGGAATGC TGCAACACCA
    GCCAAGACAC CAGTGGTTGC ACAGCCCCTA ACAGCTCAGA GCTCTGAGAG CAGCAACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAATAAATG CATCCTTGAA CCCTACAACA
    TCAGCGAGCC AAGCTTTTGC CACTATGAAG CCAACTGCCA AACAGGACTG TGACAGTAGT
    GACACAAGTG AAGAAGCCGA GGGCAAATCC AGGAGTCTGG TGGGGCAACC ATCGGTTACC
    ATTATCTCGG AGACCAGTGA TACCACTGAT ATTGATGAGG TAGTGTCTCC AACTCCTGCT
    CCAATCAAGG CAACCAAAGG AAAGGCTGTA CCCATATTGG CAAAGACTGT CACACCATCC
    AGCCTAAAAG CTACCAAAAG CAAAGCCAGC AGTGCAGCTA AAACTGCCAA AGCAGTGCAG
    CAAATAGCTG TAGCCAACTC TGACAGCAGT GATTCTTTAG ATAGCGATGA GGATCCTGGA
    GTAAAGCAGA CACCATCTGT ACCATTAAAG AAAGTAAAGT CAGGGAAAGG AGCTAAACTG
    ACTGCCGTGT GTGTCAGTGG TGGAAATCAG AAACTGCCCA GTTCGTTACC TGTGCCCTCT
    TTACAAAAGC CCAGTGTATC TGACGTTTCT GAGCCCAGCG ACTCGTCCGA TAGTGACATC
    GATACAGGAG CCCAGTCTCT TGTAGCACCA GTACATGCGG TGCCCACCCC ACAGAAACGG
    AAGAGGAATC CAAAAGTCAA AGCTCAAGTG ACGCAACCTC CAGCTGCTTC AGTGAAACAC
    AAAAAAAAGT CTAAAGCAGA GCAACTGGCA GCATCTGCAC AGCCAGCACC AGAGCCAGTG
    ATCGACTCTG AAGAGGAGAC CATAATGGCT CTCCTTTCGG GTCAGAGCCC AAAGAAGGGG
    AAGCAGAAAC TATCCAAACT GGGACTGAAA GCAGCTCCTT CAGCCACTCC CCTTCCTGTA
    CAAAAGAATG GCAGTGTGTC GGACTCATCC GATGACAGCG ATATCGGAAC TGCAACATTA
    AATATAGTTA CACCTCCAGC GTTGGAGCTC AGGCCGCCGA CCGCTGACAA AGAAAAGAAA
    AGTGCAAAGA AGAGGAAGAG CTCCTCAGAA GATGGACCCA AAAAGAAAGC TAAGAAAGCA
    AAGAAGGAAG GAAAGAAAGA AAAGAAGAAA TCAAAAGCAG AAGGAGGAGA GGCTGGAGCC
    AGCACCCCTA AAGCAAAGTC CTCCAAAAAA GATAAAAAGA AGCTAGAGAA ATCAGAGAAA
    AAGAAATCAA GCAAAAAAAA AAAAAGGAAA AGAAAAAGAA GGATGGGGAA AAAAAGGAGG
    CAAAGAAGAC CAAGAAGGGT TCAGCAACTG GCGTCTCCCC TAAACCAAAG AAAAAGAAGA
    AAAGTAAAGC GGATAAAGTT GACTGAGGAA CACAAGGAAG GAGGGCCTGA TATCTACCCG
    CCAGGTACAG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947456.1
    CDS161..4615
    Translation

    Target ORF information:

    RefSeq Version XM_018091967.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091967.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC TTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGA AGACTGCGGC ACCTCCCCAG
    GCATCCCAGA AAAAGGGCAA ATCGCCTCTC TTGGCCAGTA CAGGGAATGC TGCAACACCA
    GCCAAGACAC CAGTGGTTGC ACAGCCCCTA ACAGCTCAGA GCTCTGAGAG CAGCAACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAATAAATG CATCCTTGAA CCCTACAACA
    TCAGCGAGCC AAGCTTTTGC CACTATGAAG CCAACTGCCA AACAGGACTG TGACAGTAGT
    GACACAAGTG AAGAAGCCGA GGGCAAATCC AGGAGTCTGG TGGGGCAACC ATCGGTTACC
    ATTATCTCGG AGACCAGTGA TACCACTGAT ATTGATGAGG TAGTGTCTCC AACTCCTGCT
    CCAATCAAGG CAACCAAAGG AAAGGCTGTA CCCATATTGG CAAAGACTGT CACACCATCC
    AGCCTAAAAG CTACCAAAAG CAAAGCCAGC AGTGCAGCTA AAACTGCCAA AGCAGTGCAG
    CAAATAGCTG TAGCCAACTC TGACAGCAGT GATTCTTTAG ATAGCGATGA GGATCCTGGA
    GTAAAGCAGA CACCATCTGT ACCATTAAAG AAAGTAAAGT CAGGGAAAGG AGCTAAACTG
    ACTGCCGTGT GTGTCAGTGG TGGAAATCAG AAACTGCCCA GTTCGTTACC TGTGCCCTCT
    TTACAAAAGC CCAGTGTATC TGACGTTTCT GAGCCCAGCG ACTCGTCCGA TAGTGACATC
    GATACAGGAG CCCAGTCTCT TGTAGCACCA GTACATGCGG TGCCCACCCC ACAGAAACGG
    AAGAGGAATC CAAAAGTCAA AGCTCAAGTG ACGCAACCTC CAGCTGCTTC AGTGAAACAC
    AAAAAAAAGT CTAAAGCAGA GCAACTGGCA GCATCTGCAC AGCCAGCACC AGAGCCAGTG
    ATCGACTCTG AAGAGGAGAC CATAATGGCT CTCCTTTCGG GTCAGAGCCC AAAGAAGGGG
    AAGCAGAAAC TATCCAAACT GGGACTGAAA GCAGCTCCTT CAGCCACTCC CCTTCCTGTA
    CAAAAGAATG GCAGTGTGTC GGACTCATCC GATGACAGCG ATATCGGAAC TGCAACATTA
    AATATAGTTA CACCTCCAGC GTTGGAGCTC AGGCCGCCGA CCGCTGACAA AGAAAAGAAA
    AGTGCAAAGA AGAGGAAGAG CTCCTCAGAA GATGGACCCA AAAAGAAAGC TAAGAAAGCA
    AAGAAGGAAG GAAAGAAAGA AAAGAAGAAA TCAAAAGCAG AAGGAGGAGA GGCTGGAGCC
    AGCACCCCTA AAGCAAAGTC CTCCAAAAAA GATAAAAAGA AGCTAGAGAA ATCAGAGAAA
    AAGAAATCAA GCAAAAAAAA AAAAAGGAAA AGAAAAAGAA GGATGGGGAA AAAAAGGAGG
    CAAAGAAGAC CAAGAAGGGT TCAGCAACTG GCGTCTCCCC TAAACCAAAG AAAAAGAAGA
    AAAGTAAAGC GGATAAAGTT GACTGAGGAA CACAAGGAAG GAGGGCCTGA TATCTACCCG
    CCAGGTACAG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43054
    Clone ID Related Accession (Same CDS sequence) XM_018091968.1
    Accession Version XM_018091968.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC TTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGC AGAAGACTGC GGCACCTCCC
    CAGGCATCCC AGAAAAAGGG CAAATCGCCT CTCTTGGCCA GTACAGGGAA TGCTGCAACA
    CCAGCCAAGA CACCAGTGGT TGCACAGCCC CTAACAGCTC AGAGCTCTGA GAGCAGCAAC
    TCGTCAGACA GTGAGGAGGA GCCTGATGCT AAGAAAATAA ATGCATCCTT GAACCCTACA
    ACATCAGCGA GCCAAGCTTT TGCCACTATG AAGCCAACTG CCAAACAGGA CTGTGACAGT
    AGTGACACAA GTGAAGAAGC CGAGGGCAAA TCCAGGAGTC TGGTGGGGCA ACCATCGGTT
    ACCATTATCT CGGAGACCAG TGATACCACT GATATTGATG AGGTAGTGTC TCCAACTCCT
    GCTCCAATCA AGGCAACCAA AGGAAAGGCT GTACCCATAT TGGCAAAGAC TGTCACACCA
    TCCAGCCTAA AAGCTACCAA AAGCAAAGCC AGCAGTGCAG CTAAAACTGC CAAAGCAGTG
    CAGCAAATAG CTGTAGCCAA CTCTGACAGC AGTGATTCTT TAGATAGCGA TGAGGATCCT
    GGAGTAAAGC AGACACCATC TGTACCATTA AAGAAAGTAA AGTCAGGGAA AGGAGCTAAA
    CTGACTGCCG TGTGTGTCAG TGGTGGAAAT CAGAAACTGC CCAGTTCGTT ACCTGTGCCC
    TCTTTACAAA AGCCCAGTGT ATCTGACGTT TCTGAGCCCA GCGACTCGTC CGATAGTGAC
    ATCGATACAG GAGCCCAGTC TCTTGTAGCA CCAGTACATG CGGTGCCCAC CCCACAGAAA
    CGGAAGAGGA ATCCAAAAGT CAAAGCTCAA GTGACGCAAC CTCCAGCTGC TTCAGTGAAA
    CACAAAAAAA AGTCTAAAGC AGAGCAACTG GCAGCATCTG CACAGCCAGC ACCAGAGCCA
    GTGATCGACT CTGAAGAGGA GACCATAATG GCTCTCCTTT CGGGTCAGAG CCCAAAGAAG
    GGGAAGCAGA AACTATCCAA ACTGGGACTG AAAGCAGCTC CTTCAGCCAC TCCCCTTCCT
    GTACAAAAGA ATGGCAGTGT GTCGGACTCA TCCGATGACA GCGATATCGG AACTGCAACA
    TTAAATATAG TTACACCTCC AGCGTTGGAG CTCAGGCCGC CGACCGCTGA CAAAGAAAAG
    AAAAGTGCAA AGAAGAGGAA GAGCTCCTCA GAAGATGGAC CCAAAAAGAA AGCTAAGAAA
    GCAAAGAAGG AAGGAAAGAA AGAAAAGAAG AAATCAAAAG CAGAAGGAGG AGAGGCTGGA
    GCCAGCACCC CTAAAGCAAA GTCCTCCAAA AAAGATAAAA AGAAGCTAGA GAAATCAGAG
    AAAAAGAAAT CAAGCAAAAA AAAAAAGGAA AAGAAAAAGA AGGATGGGGA AAAAAAGGAG
    GCAAAGAAGA CCAAGAAGGG TTCAGCAACT GGCGTCTCCC CTAAACCAAA GAAAAAGGGC
    CTGATATCTA CCCGCCAGGT ACAGAATGAC AGAAGGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947457.1
    CDS162..4583
    Translation

    Target ORF information:

    RefSeq Version XM_018091968.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091968.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC TTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGC AGAAGACTGC GGCACCTCCC
    CAGGCATCCC AGAAAAAGGG CAAATCGCCT CTCTTGGCCA GTACAGGGAA TGCTGCAACA
    CCAGCCAAGA CACCAGTGGT TGCACAGCCC CTAACAGCTC AGAGCTCTGA GAGCAGCAAC
    TCGTCAGACA GTGAGGAGGA GCCTGATGCT AAGAAAATAA ATGCATCCTT GAACCCTACA
    ACATCAGCGA GCCAAGCTTT TGCCACTATG AAGCCAACTG CCAAACAGGA CTGTGACAGT
    AGTGACACAA GTGAAGAAGC CGAGGGCAAA TCCAGGAGTC TGGTGGGGCA ACCATCGGTT
    ACCATTATCT CGGAGACCAG TGATACCACT GATATTGATG AGGTAGTGTC TCCAACTCCT
    GCTCCAATCA AGGCAACCAA AGGAAAGGCT GTACCCATAT TGGCAAAGAC TGTCACACCA
    TCCAGCCTAA AAGCTACCAA AAGCAAAGCC AGCAGTGCAG CTAAAACTGC CAAAGCAGTG
    CAGCAAATAG CTGTAGCCAA CTCTGACAGC AGTGATTCTT TAGATAGCGA TGAGGATCCT
    GGAGTAAAGC AGACACCATC TGTACCATTA AAGAAAGTAA AGTCAGGGAA AGGAGCTAAA
    CTGACTGCCG TGTGTGTCAG TGGTGGAAAT CAGAAACTGC CCAGTTCGTT ACCTGTGCCC
    TCTTTACAAA AGCCCAGTGT ATCTGACGTT TCTGAGCCCA GCGACTCGTC CGATAGTGAC
    ATCGATACAG GAGCCCAGTC TCTTGTAGCA CCAGTACATG CGGTGCCCAC CCCACAGAAA
    CGGAAGAGGA ATCCAAAAGT CAAAGCTCAA GTGACGCAAC CTCCAGCTGC TTCAGTGAAA
    CACAAAAAAA AGTCTAAAGC AGAGCAACTG GCAGCATCTG CACAGCCAGC ACCAGAGCCA
    GTGATCGACT CTGAAGAGGA GACCATAATG GCTCTCCTTT CGGGTCAGAG CCCAAAGAAG
    GGGAAGCAGA AACTATCCAA ACTGGGACTG AAAGCAGCTC CTTCAGCCAC TCCCCTTCCT
    GTACAAAAGA ATGGCAGTGT GTCGGACTCA TCCGATGACA GCGATATCGG AACTGCAACA
    TTAAATATAG TTACACCTCC AGCGTTGGAG CTCAGGCCGC CGACCGCTGA CAAAGAAAAG
    AAAAGTGCAA AGAAGAGGAA GAGCTCCTCA GAAGATGGAC CCAAAAAGAA AGCTAAGAAA
    GCAAAGAAGG AAGGAAAGAA AGAAAAGAAG AAATCAAAAG CAGAAGGAGG AGAGGCTGGA
    GCCAGCACCC CTAAAGCAAA GTCCTCCAAA AAAGATAAAA AGAAGCTAGA GAAATCAGAG
    AAAAAGAAAT CAAGCAAAAA AAAAAAGGAA AAGAAAAAGA AGGATGGGGA AAAAAAGGAG
    GCAAAGAAGA CCAAGAAGGG TTCAGCAACT GGCGTCTCCC CTAAACCAAA GAAAAAGGGC
    CTGATATCTA CCCGCCAGGT ACAGAATGAC AGAAGGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43055
    Clone ID Related Accession (Same CDS sequence) XM_018091969.1
    Accession Version XM_018091969.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC TTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGC AGAAGACTGC GGCACCTCCC
    CAGGCATCCC AGAAAAAGGG CAAATCGCCT CTCTTGGCCA GTACAGGGAA TGCTGCAACA
    CCAGCCAAGA CACCAGTGGT TGCACAGCCC CTAACAGCTC AGAGCTCTGA GAGCAGCAAC
    TCGTCAGACA GTGAGGAGGA GCCTGATGCT AAGAAAATAA ATGCATCCTT GAACCCTACA
    ACATCAGCGA GCCAAGCTTT TGCCACTATG AAGCCAACTG CCAAACAGGA CTGTGACAGT
    AGTGACACAA GTGAAGAAGC CGAGGGCAAA TCCAGGAGTC TGGTGGGGCA ACCATCGGTT
    ACCATTATCT CGGAGACCAG TGATACCACT GATATTGATG AGGTAGTGTC TCCAACTCCT
    GCTCCAATCA AGGCAACCAA AGGAAAGGCT GTACCCATAT TGGCAAAGAC TGTCACACCA
    TCCAGCCTAA AAGCTACCAA AAGCAAAGCC AGCAGTGCAG CTAAAACTGC CAAAGCAGTG
    CAGCAAATAG CTGTAGCCAA CTCTGACAGC AGTGATTCTT TAGATAGCGA TGAGGATCCT
    GGAGTAAAGC AGACACCATC TGTACCATTA AAGAAAGTAA AGTCAGGGAA AGGAGCTAAA
    CTGACTGCCG TGTGTGTCAG TGGTGGAAAT CAGAAACTGC CCAGTTCGTT ACCTGTGCCC
    TCTTTACAAA AGCCCAGTGT ATCTGACGTT TCTGAGCCCA GCGACTCGTC CGATAGTGAC
    ATCGATACAG GAGCCCAGTC TCTTGTAGCA CCAGTACATG CGGTGCCCAC CCCACAGAAA
    CGGAAGAGGA ATCCAAAAGT CAAAGCTCAA GTGACGCAAC CTCCAGCTGC TTCAGTGAAA
    CACAAAAAAA AGTCTAAAGC AGAGCAACTG GCAGCATCTG CACAGCCAGC ACCAGAGCCA
    GTGATCGACT CTGAAGAGGA GACCATAATG GCTCTCCTTT CGGGTCAGAG CCCAAAGAAG
    GGGAAGCAGA AACTATCCAA ACTGGGACTG AAAGCAGCTC CTTCAGCCAC TCCCCTTCCT
    GTACAAAAGA ATGGCAGTGT GTCGGACTCA TCCGATGACA GCGATATCGG AACTGCAACA
    TTAAATATAG TTACACCTCC AGCGTTGGAG CTCAGGCCGC CGACCGCTGA CAAAGAAAAG
    AAAAGTGCAA AGAAGAGGAA GAGCTCCTCA GAAGATGGAC CCAAAAAGAA AGCTAAGAAA
    GCAAAGAAGG AAGGAAAGAA AGAAAAGAAG AAATCAAAAG CAGAAGGAGG AGAGGCTGGA
    GCCAGCACCC CTAAAGCAAA GTCCTCCAAA AAAGATAAAA AGAAGCTAGA GAAATCAGAG
    AAAAAGAAAT CAAGCAAAAA AAAAAAAAGG AAAAGAAAAA GAAGGATGGG GAAAAAAAGG
    AGGCAAAGAA GACCAAGAAG GGTTCAGCAA CTGGCGTCTC CCCTAAACCA AAGAAAAAGG
    GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947458.1
    CDS162..4547
    Translation

    Target ORF information:

    RefSeq Version XM_018091969.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091969.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC TTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGC AGAAGACTGC GGCACCTCCC
    CAGGCATCCC AGAAAAAGGG CAAATCGCCT CTCTTGGCCA GTACAGGGAA TGCTGCAACA
    CCAGCCAAGA CACCAGTGGT TGCACAGCCC CTAACAGCTC AGAGCTCTGA GAGCAGCAAC
    TCGTCAGACA GTGAGGAGGA GCCTGATGCT AAGAAAATAA ATGCATCCTT GAACCCTACA
    ACATCAGCGA GCCAAGCTTT TGCCACTATG AAGCCAACTG CCAAACAGGA CTGTGACAGT
    AGTGACACAA GTGAAGAAGC CGAGGGCAAA TCCAGGAGTC TGGTGGGGCA ACCATCGGTT
    ACCATTATCT CGGAGACCAG TGATACCACT GATATTGATG AGGTAGTGTC TCCAACTCCT
    GCTCCAATCA AGGCAACCAA AGGAAAGGCT GTACCCATAT TGGCAAAGAC TGTCACACCA
    TCCAGCCTAA AAGCTACCAA AAGCAAAGCC AGCAGTGCAG CTAAAACTGC CAAAGCAGTG
    CAGCAAATAG CTGTAGCCAA CTCTGACAGC AGTGATTCTT TAGATAGCGA TGAGGATCCT
    GGAGTAAAGC AGACACCATC TGTACCATTA AAGAAAGTAA AGTCAGGGAA AGGAGCTAAA
    CTGACTGCCG TGTGTGTCAG TGGTGGAAAT CAGAAACTGC CCAGTTCGTT ACCTGTGCCC
    TCTTTACAAA AGCCCAGTGT ATCTGACGTT TCTGAGCCCA GCGACTCGTC CGATAGTGAC
    ATCGATACAG GAGCCCAGTC TCTTGTAGCA CCAGTACATG CGGTGCCCAC CCCACAGAAA
    CGGAAGAGGA ATCCAAAAGT CAAAGCTCAA GTGACGCAAC CTCCAGCTGC TTCAGTGAAA
    CACAAAAAAA AGTCTAAAGC AGAGCAACTG GCAGCATCTG CACAGCCAGC ACCAGAGCCA
    GTGATCGACT CTGAAGAGGA GACCATAATG GCTCTCCTTT CGGGTCAGAG CCCAAAGAAG
    GGGAAGCAGA AACTATCCAA ACTGGGACTG AAAGCAGCTC CTTCAGCCAC TCCCCTTCCT
    GTACAAAAGA ATGGCAGTGT GTCGGACTCA TCCGATGACA GCGATATCGG AACTGCAACA
    TTAAATATAG TTACACCTCC AGCGTTGGAG CTCAGGCCGC CGACCGCTGA CAAAGAAAAG
    AAAAGTGCAA AGAAGAGGAA GAGCTCCTCA GAAGATGGAC CCAAAAAGAA AGCTAAGAAA
    GCAAAGAAGG AAGGAAAGAA AGAAAAGAAG AAATCAAAAG CAGAAGGAGG AGAGGCTGGA
    GCCAGCACCC CTAAAGCAAA GTCCTCCAAA AAAGATAAAA AGAAGCTAGA GAAATCAGAG
    AAAAAGAAAT CAAGCAAAAA AAAAAAAAGG AAAAGAAAAA GAAGGATGGG GAAAAAAAGG
    AGGCAAAGAA GACCAAGAAG GGTTCAGCAA CTGGCGTCTC CCCTAAACCA AAGAAAAAGG
    GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43056
    Clone ID Related Accession (Same CDS sequence) XM_018091971.1
    Accession Version XM_018091971.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC TTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGA AGACTGCGGC ACCTCCCCAG
    GCATCCCAGA AAAAGGGCAA ATCGCCTCTC TTGGCCAGTA CAGGGAATGC TGCAACACCA
    GCCAAGACAC CAGTGGTTGC ACAGCCCCTA ACAGCTCAGA GCTCTGAGAG CAGCAACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG GTAGTGTCTC CAACTCCTGC TCCAATCAAG
    GCAACCAAAG GAAAGGCTGT ACCCATATTG GCAAAGACTG TCACACCATC CAGCCTAAAA
    GCTACCAAAA GCAAAGCCAG CAGTGCAGCT AAAACTGCCA AAGCAGTGCA GCAAATAGCT
    GTAGCCAACT CTGACAGCAG TGATTCTTTA GATAGCGATG AGGATCCTGG AGTAAAGCAG
    ACACCATCTG TACCATTAAA GAAAGTAAAG TCAGGGAAAG GAGCTAAACT GACTGCCGTG
    TGTGTCAGTG GTGGAAATCA GAAACTGCCC AGTTCGTTAC CTGTGCCCTC TTTACAAAAG
    CCCAGTGTAT CTGACGTTTC TGAGCCCAGC GACTCGTCCG ATAGTGACAT CGATACAGGA
    GCCCAGTCTC TTGTAGCACC AGTACATGCG GTGCCCACCC CACAGAAACG GAAGAGGAAT
    CCAAAAGTCA AAGCTCAAGT GACGCAACCT CCAGCTGCTT CAGTGAAACA CAAAAAAAAG
    TCTAAAGCAG AGCAACTGGC AGCATCTGCA CAGCCAGCAC CAGAGCCAGT GATCGACTCT
    GAAGAGGAGA CCATAATGGC TCTCCTTTCG GGTCAGAGCC CAAAGAAGGG GAAGCAGAAA
    CTATCCAAAC TGGGACTGAA AGCAGCTCCT TCAGCCACTC CCCTTCCTGT ACAAAAGAAT
    GGCAGTGTGT CGGACTCATC CGATGACAGC GATATCGGAA CTGCAACATT AAATATAGTT
    ACACCTCCAG CGTTGGAGCT CAGGCCGCCG ACCGCTGACA AAGAAAAGAA AAGTGCAAAG
    AAGAGGAAGA GCTCCTCAGA AGATGGACCC AAAAAGAAAG CTAAGAAAGC AAAGAAGGAA
    GGAAAGAAAG AAAAGAAGAA ATCAAAAGCA GAAGGAGGAG AGGCTGGAGC CAGCACCCCT
    AAAGCAAAGT CCTCCAAAAA AGATAAAAAG AAGCTAGAGA AATCAGAGAA AAAGAAATCA
    AGCAAAAAAA AAAAAAGGAA AAGAAAAAGA AGGATGGGGA AAAAAAGGAG GCAAAGAAGA
    CCAAGAAGGG TTCAGCAACT GGCGTCTCCC CTAAACCAAA GAAAAAGAAG AAAAGTAAAG
    CGGATAAAGT TGACTGAGGA ACACAAGGAA GGAGGGCCTG ATATCTACCC GCCAGGTACA
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947460.1
    CDS161..4426
    Translation

    Target ORF information:

    RefSeq Version XM_018091971.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091971.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA ATGTTGTAGG GAAGAAGCTC ACAGCAAATA ATTTGCCAGC AACACCAGGC
    TCTGTCAAGA AACCTCTGCC TAATGCAGCA CCTAATGGGA AAGTAGCAAG TCAAGCCAAG
    GCCATTGTTA AACCAGATTT AAAGAACATT TCTGAGAGCA GTGATTCTTC AGACTCAGAA
    GAAGAGAATG AAAGTCAGAC ACATACAGTT TTGGCTCATG TTCATGCACC ACCAAGGACA
    CCTCTGCTAT CTCCTTCCAA AACTGCAAGG GCTGCTGTGC CAGCAAAAGC TGCTGTTGTC
    ACTAAACCAG TAGATATCGA TGAGTCGGAG AGCAGTGATT CATCGGACAG TGAAATCATA
    GTTACCCAGG CATCCCAGAA AAAGCCCAGT GATCCTACAG CCGCTAAGCC ACTAGTGACA
    AAAACACCCA ACTTGGCCCA ACCTGGAAAG GTGGCAACGC CAACAAAGAC ACCAGTGAGT
    GCAAAGCCCC TAACAGCTCA GAGCTCTGAG AGCAGCGACT CGTCAGACAG TGAGGAGGAG
    CTTAATGCTA AGAATACAGC AGCACCTCTC CAGGCATCCC AGAAAAAGAC TATTGTTCCT
    CCCACATCAA CAAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC CGGAACATTG
    ACAACACCAG CCAAGGCATC TGTGGTTGCT CAGAGCTCTG AGAGCAGCGA CTCGTCAGAC
    AGTGAGTTGG AGCCTGATGC TAAGAAAACA GCAGCGCCTC TCCAAGGATC CCAAAAAAAT
    ATACCTGCCA CACCTTCTAC AGCCACTAGG CCACCAGGGA CAAAAACACC CCTCTTGGCC
    AAACCTGGGA AACTGGCAAC ACCAACCAAG GCATCTGTGG TTGCTAAGCC ACTGGCAGCT
    CAAAGCTCTG AGAGCAGTGA CTCGTCCGAC AGTGAGGAGG AGCCTGATAC TAAGAAGACA
    GCCGTGCCTC TCCAGGGATC TCAGAAAAAA ACACCTGTCA CACCTTCCAC AGCCGCTAGA
    CCACCAGGGA CAAAAACACC CCTCTTGGCC AAACCCGGAA CATTGGCAAC ACCAGCCAAA
    GCATCTGTAG TTCCTCAGAG CTCTGAGAGC AGTGACTCGT CAGACAGTGA GTTGGAGCCT
    GATGCTAAGA AAACAGCAGC GCCTCTCCAA GGATCCCAAA AAAATATACC TGCCACACCT
    TCTACAGCCA CTAGGCCACC AGGGACAAAA ACACCCCTCT TGGCCAAACC TGGGAAACTG
    GCAACACCAA CCAAGGCATC TGTGGTTGCT CAGCCACTGG CAGCTCAAAG CTCTGAGAGC
    AGCGACTCGT CCGACAGTGA GGAGGAGCCT GATACTAAGA AGACAGCCGT GCCTCTCCAG
    GGATCTCAGA AAAAAACACC TGTCACACCT TCCACAGCCG CTAGACCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC CGGAACATTG GCAACACCAG CCAAGGCATC TGTAGTTGCT
    CAGAGCTCTG AGAGCAGTGA CTCGTCAGAC AGTGAGTTGG AGCCTGATGC TAAGAAAACA
    GCAGCTTCTC TCCAAGGATC CCAGAAAAAT ACACCAGCCA CTAGGCCACC AGGGACAAAA
    ACACCCCTCT TGGCCAAACC TGGAAATTTG GCAACACCAG CCAAGGCATC TATGGTTGCT
    CAGCCACCGG CAGCTCAGAG CTCTGAGAGC AGCGACTTGT CAGACAGTGA GGAGGAACCT
    AATGCTAAGA AAACAGCAGC GCCTCTCCAG GGATCCCAGA AAAAGACACC TGCCACAGCC
    GCTAGGCCAC CAGAGACAAA AGCACCCCTC TTGGCCAAAC CTGGAAAACT GGCAACACCC
    ACCAAGGCAT CTGTGGTTGC ACAGCCACTG GCAGCTCAGA GCTCTGAGAG CAGCGACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG AAAACAGCAG CGCCTCTCCA GGGATCCCAG
    AAAAAGACAC TTGCCACACC TTCCACAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGACTAAAC CTGGAAAACT TGCAACACCA ACCAAGACAC CAGTGATTTC TTTGCCGTTA
    GCAGAAAGTA GTGACTCGTC AGAAAGTGAA GACGAGCCAG CAGTCAAGAA TGCACAACTA
    TCTGTCGTGG CGTCCAAGAA CAATAGCAAA ACGACTCTCT TGGCCAAGAC AGGGAAGTTG
    GCTGCTCCAG CCAAGACGCC AGCGGTTACA CAGCCGCCAG TAGCCGAGAG CTCTGAGAGC
    AGCAACTCAT CTGATAGTGA GGAGGAGCTT AACAATAAGA AGACTGCGGC ACCTCCCCAG
    GCATCCCAGA AAAAGGGCAA ATCGCCTCTC TTGGCCAGTA CAGGGAATGC TGCAACACCA
    GCCAAGACAC CAGTGGTTGC ACAGCCCCTA ACAGCTCAGA GCTCTGAGAG CAGCAACTCG
    TCAGACAGTG AGGAGGAGCC TGATGCTAAG GTAGTGTCTC CAACTCCTGC TCCAATCAAG
    GCAACCAAAG GAAAGGCTGT ACCCATATTG GCAAAGACTG TCACACCATC CAGCCTAAAA
    GCTACCAAAA GCAAAGCCAG CAGTGCAGCT AAAACTGCCA AAGCAGTGCA GCAAATAGCT
    GTAGCCAACT CTGACAGCAG TGATTCTTTA GATAGCGATG AGGATCCTGG AGTAAAGCAG
    ACACCATCTG TACCATTAAA GAAAGTAAAG TCAGGGAAAG GAGCTAAACT GACTGCCGTG
    TGTGTCAGTG GTGGAAATCA GAAACTGCCC AGTTCGTTAC CTGTGCCCTC TTTACAAAAG
    CCCAGTGTAT CTGACGTTTC TGAGCCCAGC GACTCGTCCG ATAGTGACAT CGATACAGGA
    GCCCAGTCTC TTGTAGCACC AGTACATGCG GTGCCCACCC CACAGAAACG GAAGAGGAAT
    CCAAAAGTCA AAGCTCAAGT GACGCAACCT CCAGCTGCTT CAGTGAAACA CAAAAAAAAG
    TCTAAAGCAG AGCAACTGGC AGCATCTGCA CAGCCAGCAC CAGAGCCAGT GATCGACTCT
    GAAGAGGAGA CCATAATGGC TCTCCTTTCG GGTCAGAGCC CAAAGAAGGG GAAGCAGAAA
    CTATCCAAAC TGGGACTGAA AGCAGCTCCT TCAGCCACTC CCCTTCCTGT ACAAAAGAAT
    GGCAGTGTGT CGGACTCATC CGATGACAGC GATATCGGAA CTGCAACATT AAATATAGTT
    ACACCTCCAG CGTTGGAGCT CAGGCCGCCG ACCGCTGACA AAGAAAAGAA AAGTGCAAAG
    AAGAGGAAGA GCTCCTCAGA AGATGGACCC AAAAAGAAAG CTAAGAAAGC AAAGAAGGAA
    GGAAAGAAAG AAAAGAAGAA ATCAAAAGCA GAAGGAGGAG AGGCTGGAGC CAGCACCCCT
    AAAGCAAAGT CCTCCAAAAA AGATAAAAAG AAGCTAGAGA AATCAGAGAA AAAGAAATCA
    AGCAAAAAAA AAAAAAGGAA AAGAAAAAGA AGGATGGGGA AAAAAAGGAG GCAAAGAAGA
    CCAAGAAGGG TTCAGCAACT GGCGTCTCCC CTAAACCAAA GAAAAAGAAG AAAAGTAAAG
    CGGATAAAGT TGACTGAGGA ACACAAGGAA GGAGGGCCTG ATATCTACCC GCCAGGTACA
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43057
    Clone ID Related Accession (Same CDS sequence) XM_018091972.1
    Accession Version XM_018091972.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4233bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product treacle protein isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA CACATACAGT TTTGGCTCAT GTTCATGCAC CACCAAGGAC ACCTCTGCTA
    TCTCCTTCCA AAACTGCAAG GGCTGCTGTG CCAGCAAAAG CTGCTGTTGT CACTAAACCA
    GTAGATATCG ATGAGTCGGA GAGCAGTGAT TCATCGGACA GTGAAATCAT AGTTACCCAG
    GCATCCCAGA AAAAGCCCAG TGATCCTACA GCCGCTAAGC CACTAGTGAC AAAAACACCC
    AACTTGGCCC AACCTGGAAA GGTGGCAACG CCAACAAAGA CACCAGTGAG TGCAAAGCCC
    CTAACAGCTC AGAGCTCTGA GAGCAGCGAC TCGTCAGACA GTGAGGAGGA GCTTAATGCT
    AAGAATACAG CAGCACCTCT CCAGGCATCC CAGAAAAAGA CTATTGTTCC TCCCACATCA
    ACAAGGCCAC CAGGGACAAA AACACCCCTC TTGGCCAAAC CCGGAACATT GACAACACCA
    GCCAAGGCAT CTGTGGTTGC TCAGAGCTCT GAGAGCAGCG ACTCGTCAGA CAGTGAGTTG
    GAGCCTGATG CTAAGAAAAC AGCAGCGCCT CTCCAAGGAT CCCAAAAAAA TATACCTGCC
    ACACCTTCTA CAGCCACTAG GCCACCAGGG ACAAAAACAC CCCTCTTGGC CAAACCTGGG
    AAACTGGCAA CACCAACCAA GGCATCTGTG GTTGCTAAGC CACTGGCAGC TCAAAGCTCT
    GAGAGCAGTG ACTCGTCCGA CAGTGAGGAG GAGCCTGATA CTAAGAAGAC AGCCGTGCCT
    CTCCAGGGAT CTCAGAAAAA AACACCTGTC ACACCTTCCA CAGCCGCTAG ACCACCAGGG
    ACAAAAACAC CCCTCTTGGC CAAACCCGGA ACATTGGCAA CACCAGCCAA AGCATCTGTA
    GTTCCTCAGA GCTCTGAGAG CAGTGACTCG TCAGACAGTG AGTTGGAGCC TGATGCTAAG
    AAAACAGCAG CGCCTCTCCA AGGATCCCAA AAAAATATAC CTGCCACACC TTCTACAGCC
    ACTAGGCCAC CAGGGACAAA AACACCCCTC TTGGCCAAAC CTGGGAAACT GGCAACACCA
    ACCAAGGCAT CTGTGGTTGC TCAGCCACTG GCAGCTCAAA GCTCTGAGAG CAGCGACTCG
    TCCGACAGTG AGGAGGAGCC TGATACTAAG AAGACAGCCG TGCCTCTCCA GGGATCTCAG
    AAAAAAACAC CTGTCACACC TTCCACAGCC GCTAGACCAC CAGGGACAAA AACACCCCTC
    TTGGCCAAAC CCGGAACATT GGCAACACCA GCCAAGGCAT CTGTAGTTGC TCAGAGCTCT
    GAGAGCAGTG ACTCGTCAGA CAGTGAGTTG GAGCCTGATG CTAAGAAAAC AGCAGCTTCT
    CTCCAAGGAT CCCAGAAAAA TACACCAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGGCCAAAC CTGGAAATTT GGCAACACCA GCCAAGGCAT CTATGGTTGC TCAGCCACCG
    GCAGCTCAGA GCTCTGAGAG CAGCGACTTG TCAGACAGTG AGGAGGAACC TAATGCTAAG
    AAAACAGCAG CGCCTCTCCA GGGATCCCAG AAAAAGACAC CTGCCACAGC CGCTAGGCCA
    CCAGAGACAA AAGCACCCCT CTTGGCCAAA CCTGGAAAAC TGGCAACACC CACCAAGGCA
    TCTGTGGTTG CACAGCCACT GGCAGCTCAG AGCTCTGAGA GCAGCGACTC GTCAGACAGT
    GAGGAGGAGC CTGATGCTAA GAAAACAGCA GCGCCTCTCC AGGGATCCCA GAAAAAGACA
    CTTGCCACAC CTTCCACAGC CACTAGGCCA CCAGGGACAA AAACACCCCT CTTGACTAAA
    CCTGGAAAAC TTGCAACACC AACCAAGACA CCAGTGATTT CTTTGCCGTT AGCAGAAAGT
    AGTGACTCGT CAGAAAGTGA AGACGAGCCA GCAGTCAAGA ATGCACAACT ATCTGTCGTG
    GCGTCCAAGA ACAATAGCAA AACGACTCTC TTGGCCAAGA CAGGGAAGTT GGCTGCTCCA
    GCCAAGACGC CAGCGGTTAC ACAGCCGCCA GTAGCCGAGA GCTCTGAGAG CAGCAACTCA
    TCTGATAGTG AGGAGGAGCT TAACAATAAG CAGAAGACTG CGGCACCTCC CCAGGCATCC
    CAGAAAAAGG GCAAATCGCC TCTCTTGGCC AGTACAGGGA ATGCTGCAAC ACCAGCCAAG
    ACACCAGTGG TTGCACAGCC CCTAACAGCT CAGAGCTCTG AGAGCAGCAA CTCGTCAGAC
    AGTGAGGAGG AGCCTGATGC TAAGAAAATA AATGCATCCT TGAACCCTAC AACATCAGCG
    AGCCAAGCTT TTGCCACTAT GAAGCCAACT GCCAAACAGG ACTGTGACAG TAGTGACACA
    AGTGAAGAAG CCGAGGGCAA ATCCAGGAGT CTGGTGGGGC AACCATCGGT TACCATTATC
    TCGGAGACCA GTGATACCAC TGATATTGAT GAGGTAGTGT CTCCAACTCC TGCTCCAATC
    AAGGCAACCA AAGGAAAGGC TGTACCCATA TTGGCAAAGA CTGTCACACC ATCCAGCCTA
    AAAGCTACCA AAAGCAAAGC CAGCAGTGCA GCTAAAACTG CCAAAGCAGT GCAGCAAATA
    GCTGTAGCCA ACTCTGACAG CAGTGATTCT TTAGATAGCG ATGAGGATCC TGGAGTAAAG
    CAGACACCAT CTGTACCATT AAAGAAAGTA AAGTCAGGGA AAGGAGCTAA ACTGACTGCC
    GTGTGTGTCA GTGGTGGAAA TCAGAAACTG CCCAGTTCGT TACCTGTGCC CTCTTTACAA
    AAGCCCAGTG TATCTGACGT TTCTGAGCCC AGCGACTCGT CCGATAGTGA CATCGATACA
    GGAGCCCAGT CTCTTGTAGC ACCAGTACAT GCGGTGCCCA CCCCACAGAA ACGGAAGAGG
    AATCCAAAAG TCAAAGCTCA AGTGACGCAA CCTCCAGCTG CTTCAGTGAA ACACAAAAAA
    AAGTCTAAAG CAGAGCAACT GGCAGCATCT GCACAGCCAG CACCAGAGCC AGTGATCGAC
    TCTGAAGAGG AGACCATAAT GGCTCTCCTT TCGGGTCAGA GCCCAAAGAA GGGGAAGCAG
    AAACTATCCA AACTGGGACT GAAAGCAGCT CCTTCAGCCA CTCCCCTTCC TGTACAAAAG
    AATGGCAGTG TGTCGGACTC ATCCGATGAC AGCGATATCG GAACTGCAAC ATTAAATATA
    GTTACACCTC CAGCGTTGGA GCTCAGGCCG CCGACCGCTG ACAAAGAAAA GAAAAGTGCA
    AAGAAGAGGA AGAGCTCCTC AGAAGATGGA CCCAAAAAGA AAGCTAAGAA AGCAAAGAAG
    GAAGGAAAGA AAGAAAAGAA GAAATCAAAA GCAGAAGGAG GAGAGGCTGG AGCCAGCACC
    CCTAAAGCAA AGTCCTCCAA AAAAGATAAA AAGAAGCTAG AGAAATCAGA GAAAAAGAAA
    TCAAGCAAAA AAAAAAAGGA AAAGAAAAAG AAGGATGGGG AAAAAAAGGA GGCAAAGAAG
    ACCAAGAAGG GTTCAGCAAC TGGCGTCTCC CCTAAACCAA AGAAAAAGGG CCTGATATCT
    ACCCGCCAGG TACAGAATGA CAGAAGGGAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947461.1
    CDS162..4394
    Translation

    Target ORF information:

    RefSeq Version XM_018091972.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Treacher Collins-Franceschetti syndrome 1 (tcof1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091972.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAGGAAG TGGAGGGATC GCTGCTGGCT CTGATTCACC AGCATCTCTT GCAGGCTGGA 
    TATAGTAAGG CAGCCAGAGA GCTGCAGATG CAGAGTGGCA AGAAATTCTC TACCCCGGAA
    GTGTCGCTCC AAGATATTTA CAAAGACTGG ATTAAACACC CAGAAAAAGA CAAGACAAAG
    AAAACAGTCC AAGTAAATAA TCAGGGGGTG AAGGTACGAG TGCCAGATCC AGAGAGCAGC
    ACGGAGAGTT CTGAGGATGA ATTGGTGTTC TATTCCCCAG CTGGTAGTAG AACTCTCTCA
    AATGCAACCC CTCAAGTGCA ACAGACTAAA GAAAAAAACA ATAAAAAGAA ACCAACTGCT
    AAGGAGAAGG CAGCCACCAC TATTTCAAAG ATGTCATCAG GAGTTAATGC CACCAGTGGG
    CAGAACAAAA AGGCTGTTTC AGCCATACCA CAAGCACCAA AGGTCGTGAT GGAGAGTAGC
    AGTTCTTCTG ACAGTGAAGA GGATCAGGCA CCTCAAAAAG CTGCTCCAGT GAAAATATCC
    ACAAAGCCAG TGGCTGAGAG CACTGAAAGC TCATCTGGTT CTGAATCGGA CATGGAGACT
    ACCGGACAGA CACATACAGT TTTGGCTCAT GTTCATGCAC CACCAAGGAC ACCTCTGCTA
    TCTCCTTCCA AAACTGCAAG GGCTGCTGTG CCAGCAAAAG CTGCTGTTGT CACTAAACCA
    GTAGATATCG ATGAGTCGGA GAGCAGTGAT TCATCGGACA GTGAAATCAT AGTTACCCAG
    GCATCCCAGA AAAAGCCCAG TGATCCTACA GCCGCTAAGC CACTAGTGAC AAAAACACCC
    AACTTGGCCC AACCTGGAAA GGTGGCAACG CCAACAAAGA CACCAGTGAG TGCAAAGCCC
    CTAACAGCTC AGAGCTCTGA GAGCAGCGAC TCGTCAGACA GTGAGGAGGA GCTTAATGCT
    AAGAATACAG CAGCACCTCT CCAGGCATCC CAGAAAAAGA CTATTGTTCC TCCCACATCA
    ACAAGGCCAC CAGGGACAAA AACACCCCTC TTGGCCAAAC CCGGAACATT GACAACACCA
    GCCAAGGCAT CTGTGGTTGC TCAGAGCTCT GAGAGCAGCG ACTCGTCAGA CAGTGAGTTG
    GAGCCTGATG CTAAGAAAAC AGCAGCGCCT CTCCAAGGAT CCCAAAAAAA TATACCTGCC
    ACACCTTCTA CAGCCACTAG GCCACCAGGG ACAAAAACAC CCCTCTTGGC CAAACCTGGG
    AAACTGGCAA CACCAACCAA GGCATCTGTG GTTGCTAAGC CACTGGCAGC TCAAAGCTCT
    GAGAGCAGTG ACTCGTCCGA CAGTGAGGAG GAGCCTGATA CTAAGAAGAC AGCCGTGCCT
    CTCCAGGGAT CTCAGAAAAA AACACCTGTC ACACCTTCCA CAGCCGCTAG ACCACCAGGG
    ACAAAAACAC CCCTCTTGGC CAAACCCGGA ACATTGGCAA CACCAGCCAA AGCATCTGTA
    GTTCCTCAGA GCTCTGAGAG CAGTGACTCG TCAGACAGTG AGTTGGAGCC TGATGCTAAG
    AAAACAGCAG CGCCTCTCCA AGGATCCCAA AAAAATATAC CTGCCACACC TTCTACAGCC
    ACTAGGCCAC CAGGGACAAA AACACCCCTC TTGGCCAAAC CTGGGAAACT GGCAACACCA
    ACCAAGGCAT CTGTGGTTGC TCAGCCACTG GCAGCTCAAA GCTCTGAGAG CAGCGACTCG
    TCCGACAGTG AGGAGGAGCC TGATACTAAG AAGACAGCCG TGCCTCTCCA GGGATCTCAG
    AAAAAAACAC CTGTCACACC TTCCACAGCC GCTAGACCAC CAGGGACAAA AACACCCCTC
    TTGGCCAAAC CCGGAACATT GGCAACACCA GCCAAGGCAT CTGTAGTTGC TCAGAGCTCT
    GAGAGCAGTG ACTCGTCAGA CAGTGAGTTG GAGCCTGATG CTAAGAAAAC AGCAGCTTCT
    CTCCAAGGAT CCCAGAAAAA TACACCAGCC ACTAGGCCAC CAGGGACAAA AACACCCCTC
    TTGGCCAAAC CTGGAAATTT GGCAACACCA GCCAAGGCAT CTATGGTTGC TCAGCCACCG
    GCAGCTCAGA GCTCTGAGAG CAGCGACTTG TCAGACAGTG AGGAGGAACC TAATGCTAAG
    AAAACAGCAG CGCCTCTCCA GGGATCCCAG AAAAAGACAC CTGCCACAGC CGCTAGGCCA
    CCAGAGACAA AAGCACCCCT CTTGGCCAAA CCTGGAAAAC TGGCAACACC CACCAAGGCA
    TCTGTGGTTG CACAGCCACT GGCAGCTCAG AGCTCTGAGA GCAGCGACTC GTCAGACAGT
    GAGGAGGAGC CTGATGCTAA GAAAACAGCA GCGCCTCTCC AGGGATCCCA GAAAAAGACA
    CTTGCCACAC CTTCCACAGC CACTAGGCCA CCAGGGACAA AAACACCCCT CTTGACTAAA
    CCTGGAAAAC TTGCAACACC AACCAAGACA CCAGTGATTT CTTTGCCGTT AGCAGAAAGT
    AGTGACTCGT CAGAAAGTGA AGACGAGCCA GCAGTCAAGA ATGCACAACT ATCTGTCGTG
    GCGTCCAAGA ACAATAGCAA AACGACTCTC TTGGCCAAGA CAGGGAAGTT GGCTGCTCCA
    GCCAAGACGC CAGCGGTTAC ACAGCCGCCA GTAGCCGAGA GCTCTGAGAG CAGCAACTCA
    TCTGATAGTG AGGAGGAGCT TAACAATAAG CAGAAGACTG CGGCACCTCC CCAGGCATCC
    CAGAAAAAGG GCAAATCGCC TCTCTTGGCC AGTACAGGGA ATGCTGCAAC ACCAGCCAAG
    ACACCAGTGG TTGCACAGCC CCTAACAGCT CAGAGCTCTG AGAGCAGCAA CTCGTCAGAC
    AGTGAGGAGG AGCCTGATGC TAAGAAAATA AATGCATCCT TGAACCCTAC AACATCAGCG
    AGCCAAGCTT TTGCCACTAT GAAGCCAACT GCCAAACAGG ACTGTGACAG TAGTGACACA
    AGTGAAGAAG CCGAGGGCAA ATCCAGGAGT CTGGTGGGGC AACCATCGGT TACCATTATC
    TCGGAGACCA GTGATACCAC TGATATTGAT GAGGTAGTGT CTCCAACTCC TGCTCCAATC
    AAGGCAACCA AAGGAAAGGC TGTACCCATA TTGGCAAAGA CTGTCACACC ATCCAGCCTA
    AAAGCTACCA AAAGCAAAGC CAGCAGTGCA GCTAAAACTG CCAAAGCAGT GCAGCAAATA
    GCTGTAGCCA ACTCTGACAG CAGTGATTCT TTAGATAGCG ATGAGGATCC TGGAGTAAAG
    CAGACACCAT CTGTACCATT AAAGAAAGTA AAGTCAGGGA AAGGAGCTAA ACTGACTGCC
    GTGTGTGTCA GTGGTGGAAA TCAGAAACTG CCCAGTTCGT TACCTGTGCC CTCTTTACAA
    AAGCCCAGTG TATCTGACGT TTCTGAGCCC AGCGACTCGT CCGATAGTGA CATCGATACA
    GGAGCCCAGT CTCTTGTAGC ACCAGTACAT GCGGTGCCCA CCCCACAGAA ACGGAAGAGG
    AATCCAAAAG TCAAAGCTCA AGTGACGCAA CCTCCAGCTG CTTCAGTGAA ACACAAAAAA
    AAGTCTAAAG CAGAGCAACT GGCAGCATCT GCACAGCCAG CACCAGAGCC AGTGATCGAC
    TCTGAAGAGG AGACCATAAT GGCTCTCCTT TCGGGTCAGA GCCCAAAGAA GGGGAAGCAG
    AAACTATCCA AACTGGGACT GAAAGCAGCT CCTTCAGCCA CTCCCCTTCC TGTACAAAAG
    AATGGCAGTG TGTCGGACTC ATCCGATGAC AGCGATATCG GAACTGCAAC ATTAAATATA
    GTTACACCTC CAGCGTTGGA GCTCAGGCCG CCGACCGCTG ACAAAGAAAA GAAAAGTGCA
    AAGAAGAGGA AGAGCTCCTC AGAAGATGGA CCCAAAAAGA AAGCTAAGAA AGCAAAGAAG
    GAAGGAAAGA AAGAAAAGAA GAAATCAAAA GCAGAAGGAG GAGAGGCTGG AGCCAGCACC
    CCTAAAGCAA AGTCCTCCAA AAAAGATAAA AAGAAGCTAG AGAAATCAGA GAAAAAGAAA
    TCAAGCAAAA AAAAAAAGGA AAAGAAAAAG AAGGATGGGG AAAAAAAGGA GGCAAAGAAG
    ACCAAGAAGG GTTCAGCAAC TGGCGTCTCC CCTAAACCAA AGAAAAAGGG CCTGATATCT
    ACCCGCCAGG TACAGAATGA CAGAAGGGAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.