spice1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following spice1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spice1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa24739 NM_001128000.1
Latest version!
Xenopus tropicalis spindle and centriole associated protein 1 (spice1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa32203 XM_012956516.1
Latest version!
Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa32204 XM_012956517.1
Latest version!
Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa32202 XM_012956515.3
Latest version!
Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa32205 XM_012956518.3
Latest version!
Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa32205 XM_031895712.1
Latest version!
Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa32205 XM_031895713.1
Latest version!
Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa42460 XM_018091099.1
Latest version!
Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OXa32202 XM_012956515.2 Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa32205 XM_012956518.2 Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa24739
    Clone ID Related Accession (Same CDS sequence) NM_001128000.1
    Accession Version NM_001128000.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spindle and centriole-associated protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166242.1. ##Evidence-Data-START## Transcript exon combination :: BC166242.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTTATT TAAGAGCCGG CAGGACTTCA ACGAATGTTT CACTCGCAAA GAAGCCATCG 
    AAGGGCAAAA AGAAACCGCA GGCCAGAAAG GAATGGGATA GTACAGTCAG TGATCTCACG
    GTGCTCCGTG CCACAAATGA AGAGCTGGAG CACCGGCGGG AGATACACAG ATCCAAGAAC
    CAGTGGCTGG CTCGTTGGGA ACTCCAGAAC AAAGCGACAA ATAAAAAGTC GGAGAAAGAA
    AATGGAGGAT TTTCAGCTCT TTTGGAAAAT TCACGCCTAG CTCTAATGAA GGAGATCCTT
    TCTGATCAGT ATTTGATGAA TGATGTATTG GAGAGGTCAG ACCGAGCCCT TGCTGTAGTG
    AAGGACCTGT TTGGAGATGC TCCTCGCAGA CACACAGGAT TTCCCAGTGT GACTATGGCT
    CCTTCCTGTG ATCTGGACAC ATCCCGAGCA CCCATAGTGC AGAAGAAGGA TCCCCCGACC
    CAGCTTTCTA TTCTGAGCGA GTCAATCATG GATTCCCAGG CTATCAATGA AGTGGACAAA
    TCACTGAGTC CGGCTGAATA CAGCGACAGT GAAGCTGAGG TTACTATTAG CACGCAACCC
    AACAGAAAGG CAGAGAGGGG ACAACAGTTG CTGAGTGGGG AGGGACCAAA GAATGAGGCC
    TTTATAACCC CTTGCAAATC AGGAGGTGAC CCATCGCAAA CACACTGTGC TCTAAACGCC
    ACCACGGCAG TCAACAGAGT GAAAGTGCGC AGAACAGAGG AAGAGTCGCC AAAGCCGGAG
    GAATCAGAAT CTATTATAGG CCGCGTTCTA AATCCCCAGG GGAAGGCAAA TAAGAGAATC
    TCATCAAAAG GAAAGAAAAC CCGTAGCGCA ACCCCCTCTC ACACCAAGGA TCTGAGTGCT
    TGCAGGGAAT CCCAGCACGA CCTGACAGCC AGCAACCAGT CCAGCCTGGG CCTCCTGAAC
    TCTATGATCC AGGAAGTAGA GCAGGATCTG GCCGAGTACG AGAGGCAGAC GGGACGGGAA
    GTGATATCCG CACCCCCAGC TCATGGTCTG ACAGGATTCA CTCTCTCTCT TGTCAGCTCG
    TTGAAACGAG TGGTGTCGTA CCTGAAGGAG AGTGACCTGC TGCTGCAGCG AGAGGTGAAG
    GAGAGGCAGC ACCTGCAGGG GGAGCTAGTG GAACAGAGGC TGATGCTCGA TGCTCTGACT
    GCAGAAATTC TCACCCTGAA AGAAGGAAAC AACATCCATG AGAATCACTT ACAGACCAAA
    CAGCGCCCAG AAGTAGGAGG GGACAAGATG GCTGCCATCC CCCAAGAAAT GGAAGAGCTT
    CCTGTAGTCA GTGAAGAACC TTGCAGAACC TCAGCTGAGT GCAACCTTTC CAAGGTCAAT
    CAATTCCTGG ATTCGCAGGA TGTGCCAGAT ACAGATGATA AACACGGCAG ACTTGGGGTG
    TCCAAGGAAG AATGTGGCCC CTGTTTGTAC CCCCAGGGCA GAGCTGCGGA GGAGCCCAGA
    TTAGCCAGCT CTGTACCCTC ACGTATGTTC CAGCAAGCCG TCTTGTTATC CCCTCCCAGG
    CAGAAAACTG TCAGTGAACT CAGCAGCCAA TCAGCAGTTC CCAAGAGTTA CAAACATAGT
    AACCGCCCAG CCCAGGGGGG CCAACAAGTC AGCCATCATC CTACTGGATC TACAGAAAAG
    GAACCCCCCA GCCAAGGACT AGAAATGGGT CAAAACCAAA TAAATCGCCT TCAAAACGAG
    GACCTTGTTT CCCATATGCA GCAGCTGGCG CTTCAGAATG CAGCACTTAA GGCTCAGCTT
    GGGCAAATAC ACTTTCCTCC AGCAGGAAAT GCCCCAGAAG GGGGCAAATC TGTGCCAGCC
    GAAGAGAACC GTAATCCAGA ACCTGCTACG GATCCACCGC AGAACAAAGC AGCTCAGGTC
    CCAGGGAGCC TGGAGATGCG CATCGCAGAG TTAAACAGGC AGAGTGCAGA AGCGCGGAAC
    AAGCTCCTAA AACTTATTGA GCAGCAGAAG CAGAGCATTG TGGTTTCTCC CACATTATCT
    CCCATAACCC CCCAGGACAG GCGCACAGAT TCCTCCCTGG ATACGACCCC CCCTTCCTCC
    TGCAGGACCT CGGGCACAAG GTTCTCCAGT GCATCTAACA AGAGCACCTC TTCCATCAAC
    TCGTCTTTTG GGAGTGTGAG ATCGGCCTCT GCTGGGCGGA GGTCCCAGGC TGACAGTGAG
    AGGGGCGAGG GCTGGTTTGC TCTTTCTGCC CACGTCTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121472.1
    CDS74..2335
    Translation

    Target ORF information:

    RefSeq Version NM_001128000.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spindle and centriole associated protein 1 (spice1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001128000.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTTATT TAAGAGCCGG CAGGACTTCA ACGAATGTTT CACTCGCAAA GAAGCCATCG 
    AAGGGCAAAA AGAAACCGCA GGCCAGAAAG GAATGGGATA GTACAGTCAG TGATCTCACG
    GTGCTCCGTG CCACAAATGA AGAGCTGGAG CACCGGCGGG AGATACACAG ATCCAAGAAC
    CAGTGGCTGG CTCGTTGGGA ACTCCAGAAC AAAGCGACAA ATAAAAAGTC GGAGAAAGAA
    AATGGAGGAT TTTCAGCTCT TTTGGAAAAT TCACGCCTAG CTCTAATGAA GGAGATCCTT
    TCTGATCAGT ATTTGATGAA TGATGTATTG GAGAGGTCAG ACCGAGCCCT TGCTGTAGTG
    AAGGACCTGT TTGGAGATGC TCCTCGCAGA CACACAGGAT TTCCCAGTGT GACTATGGCT
    CCTTCCTGTG ATCTGGACAC ATCCCGAGCA CCCATAGTGC AGAAGAAGGA TCCCCCGACC
    CAGCTTTCTA TTCTGAGCGA GTCAATCATG GATTCCCAGG CTATCAATGA AGTGGACAAA
    TCACTGAGTC CGGCTGAATA CAGCGACAGT GAAGCTGAGG TTACTATTAG CACGCAACCC
    AACAGAAAGG CAGAGAGGGG ACAACAGTTG CTGAGTGGGG AGGGACCAAA GAATGAGGCC
    TTTATAACCC CTTGCAAATC AGGAGGTGAC CCATCGCAAA CACACTGTGC TCTAAACGCC
    ACCACGGCAG TCAACAGAGT GAAAGTGCGC AGAACAGAGG AAGAGTCGCC AAAGCCGGAG
    GAATCAGAAT CTATTATAGG CCGCGTTCTA AATCCCCAGG GGAAGGCAAA TAAGAGAATC
    TCATCAAAAG GAAAGAAAAC CCGTAGCGCA ACCCCCTCTC ACACCAAGGA TCTGAGTGCT
    TGCAGGGAAT CCCAGCACGA CCTGACAGCC AGCAACCAGT CCAGCCTGGG CCTCCTGAAC
    TCTATGATCC AGGAAGTAGA GCAGGATCTG GCCGAGTACG AGAGGCAGAC GGGACGGGAA
    GTGATATCCG CACCCCCAGC TCATGGTCTG ACAGGATTCA CTCTCTCTCT TGTCAGCTCG
    TTGAAACGAG TGGTGTCGTA CCTGAAGGAG AGTGACCTGC TGCTGCAGCG AGAGGTGAAG
    GAGAGGCAGC ACCTGCAGGG GGAGCTAGTG GAACAGAGGC TGATGCTCGA TGCTCTGACT
    GCAGAAATTC TCACCCTGAA AGAAGGAAAC AACATCCATG AGAATCACTT ACAGACCAAA
    CAGCGCCCAG AAGTAGGAGG GGACAAGATG GCTGCCATCC CCCAAGAAAT GGAAGAGCTT
    CCTGTAGTCA GTGAAGAACC TTGCAGAACC TCAGCTGAGT GCAACCTTTC CAAGGTCAAT
    CAATTCCTGG ATTCGCAGGA TGTGCCAGAT ACAGATGATA AACACGGCAG ACTTGGGGTG
    TCCAAGGAAG AATGTGGCCC CTGTTTGTAC CCCCAGGGCA GAGCTGCGGA GGAGCCCAGA
    TTAGCCAGCT CTGTACCCTC ACGTATGTTC CAGCAAGCCG TCTTGTTATC CCCTCCCAGG
    CAGAAAACTG TCAGTGAACT CAGCAGCCAA TCAGCAGTTC CCAAGAGTTA CAAACATAGT
    AACCGCCCAG CCCAGGGGGG CCAACAAGTC AGCCATCATC CTACTGGATC TACAGAAAAG
    GAACCCCCCA GCCAAGGACT AGAAATGGGT CAAAACCAAA TAAATCGCCT TCAAAACGAG
    GACCTTGTTT CCCATATGCA GCAGCTGGCG CTTCAGAATG CAGCACTTAA GGCTCAGCTT
    GGGCAAATAC ACTTTCCTCC AGCAGGAAAT GCCCCAGAAG GGGGCAAATC TGTGCCAGCC
    GAAGAGAACC GTAATCCAGA ACCTGCTACG GATCCACCGC AGAACAAAGC AGCTCAGGTC
    CCAGGGAGCC TGGAGATGCG CATCGCAGAG TTAAACAGGC AGAGTGCAGA AGCGCGGAAC
    AAGCTCCTAA AACTTATTGA GCAGCAGAAG CAGAGCATTG TGGTTTCTCC CACATTATCT
    CCCATAACCC CCCAGGACAG GCGCACAGAT TCCTCCCTGG ATACGACCCC CCCTTCCTCC
    TGCAGGACCT CGGGCACAAG GTTCTCCAGT GCATCTAACA AGAGCACCTC TTCCATCAAC
    TCGTCTTTTG GGAGTGTGAG ATCGGCCTCT GCTGGGCGGA GGTCCCAGGC TGACAGTGAG
    AGGGGCGAGG GCTGGTTTGC TCTTTCTGCC CACGTCTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32202
    Clone ID Related Accession (Same CDS sequence) XM_012956515.3 , XM_012956515.2
    Accession Version XM_012956515.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spindle and centriole-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956515.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGATCACT CTTCAGGTTC ACTTTCAATG TCTTATTTAA GAGCCGGCAG GACTTCAACG 
    AATGTTTCAC TCACAAAGAA GCCATCGAAG GGCAAAAAGA AACCGCAGGC CAGAAAGGAA
    TGGGATAGTA CAGTCAGTGA TCTCACGGTG CTCCGTGCCA CAAATGAAGA GCTGGAGCAC
    CGGCGGGAGA TACACAGATC CAAGAACCAG TGGCTGGCTC GTTGGGAACT CCAGAACAAA
    GCGACAAATA AAAAGTCGGA GAAAGAAAAT GGAGGATATT CAGCTCTTTT GGAAAATTCA
    CGCCTAGCTC TAATGAAGGA GATCCTTTCT GATCAGTATT TGATGAATGA TGTATTGGAG
    AGGTCAGACC GAGCCCTTGC TGTAGTGAAG GACCTGTTTG GAGATGCTCC TCGCAGACAC
    ACAGGATTTC CCAGTGTGAC TATGGCTCCT TCCTGTGATC TGGACACATC CCGAGCACCC
    ATAGTGCAGA AGAAGGATCC CCCAACCCAG CTTTCTATTC TGAGCGAGTC AATCATGGAT
    TCCCAGGCTA TCAATGAAGT GGACAAATCA CTGAGTCCGG CTGAATACAG CGACAGTGAA
    GCTGAGGTTA CTATTAGCAC GCAACCCAAC AGAAAGGCAG AGAGGGGACA ACAGTTGCTG
    AGTGGGGAGG GACCAAAGAA TGAGGCCTTT ATAACCCCTT GCAAATCAGG AGGTGACCCA
    TCGCAAACAC ACTGTGCTCT AAACGCCACC ACGGCAGTCA ACAGAGTGAA AGTGCGCAGA
    ACAGAGGAAG AGTCGCCAAA GCCGGAGGAA TCAGAATCTA TTATAGGCCG CGTTCTAAAT
    CCCCAGGGGA AGGCAAATAA GAGAATCTCA TCAAAAGGAA AGAAAACCCG TAGCGCAACC
    CCCTCTCACA CCAAGGATCT GAGTGCTTGC AGGGAATCCC AGCACGAACT GACAGCCAGC
    AACCAGTCCA GCCTGGGCCT CCTGAACTCT ATGATCCAGG AAGTAGAGCA GGATCTGGCC
    GAGTATGAGA GGCAGACAGG ACGGGAAGTG ATATCCGCAC CCCCAGCTCA TGGTCTGACA
    GGATTCACTC TCTCTCTTGT CAGCTCGTTG AAACGAGTGG TGTCGTACCT GAAGGAGAGT
    GACCTGCTGC TGCAGCGAGA GGTGAAGGAG AGGCAGCACC TGCAGGGGGA GCTAGTGGAA
    CAGAGGCTGA TGCTCGATGC TCTGACTGCA GAAATTCTCA CCCTGAAAGA AGGAAACAAC
    ATCCATGAGA ATCACTTACA GACCAAACAG CGCCCAGAAG TAGGAGGGGA CAAGATGGCT
    GCCATCCCCC AAGAAATGGA AGAGCTTCCT GTAGTCAGTG AAGAACCTTG CAGAACCTCA
    GCTGAGTGCA ACCTTTCCAA GGTCAATCAA TTCCTGGATT CGCAGGATGG GCCAGATACA
    GATGATAAAC ACGGCAGACT TGGGGTGTCC AAGGAAGAAT GTGGCCCCTG TTTGTACCCC
    CAGGGCAGAG CTGCGGAGGA GCCCAGATTA GCCAGCTCTG TACCCTCACG TATGTTCCAG
    CAAGCTGTCT TGTTATCCCC TCCCAGGCAG AAAACTGTCA GTGAACTCAG CAGCCAATCA
    GCAGTTCCCA AGAGTTACAA ACATAGTAAC CGCCCAGCCC AGGGGGGCCA ACAAGTCAGC
    CATCATCCTA CTGCATCTAC AGAAAAGGAA CCCCCCAGCC AAGGACTAGA AATGGGTCAA
    AACCAAATAA ATCGCCTTCA AAACGAGGAC CTTGTTTCCC ATATGCAGCA GCTGGCGCTT
    CAGAATGCAG CACTTAAGGC TCAGCTTGGG CAAATACACT TTCCTCCAGC AGGAAATGCC
    CCAGAAGGGG GCAAATCTGT GCCAGCCGAA GAGAACCGTA ATCCAGAACC TGCTACGGAT
    CCACCGCAGA ACAAAGCAGC TCAGGTCCCA GGGAGCCTGG AGATGCGCAT CGCAGAGTTA
    AACAGGCAGA GTGCAGAAGC GCGGAACAAG CTCCTAAAAC TTATTGAGCA GCAGAAGCAG
    AGCATTGTGG TTTCTCCCAC ATTATCTCCC ATAACCCCCC AGGACAGGCG CACAGATTCC
    TCCCTGGATA CGACCCCCCC TTCCTCCTGC AGGACCTCGG GCACAAGGTT CTCCAGTGCA
    TCTAACAAGA GCACCTCTTC CATCAACTCG TCTTTTGGGA GTGTGAGATC GGCCTCTGCT
    GGGCGGAGGT CCCAGGTACG GTATAAACCT GTATTGCTGA GCTGTAGTAC GGTGTCTGCA
    CTGGGCCAAA ATCAAGCAGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811969.1
    CDS381..2684
    Translation

    Target ORF information:

    RefSeq Version XM_012956515.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956515.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGATCACT CTTCAGGTTC ACTTTCAATG TCTTATTTAA GAGCCGGCAG GACTTCAACG 
    AATGTTTCAC TCACAAAGAA GCCATCGAAG GGCAAAAAGA AACCGCAGGC CAGAAAGGAA
    TGGGATAGTA CAGTCAGTGA TCTCACGGTG CTCCGTGCCA CAAATGAAGA GCTGGAGCAC
    CGGCGGGAGA TACACAGATC CAAGAACCAG TGGCTGGCTC GTTGGGAACT CCAGAACAAA
    GCGACAAATA AAAAGTCGGA GAAAGAAAAT GGAGGATATT CAGCTCTTTT GGAAAATTCA
    CGCCTAGCTC TAATGAAGGA GATCCTTTCT GATCAGTATT TGATGAATGA TGTATTGGAG
    AGGTCAGACC GAGCCCTTGC TGTAGTGAAG GACCTGTTTG GAGATGCTCC TCGCAGACAC
    ACAGGATTTC CCAGTGTGAC TATGGCTCCT TCCTGTGATC TGGACACATC CCGAGCACCC
    ATAGTGCAGA AGAAGGATCC CCCAACCCAG CTTTCTATTC TGAGCGAGTC AATCATGGAT
    TCCCAGGCTA TCAATGAAGT GGACAAATCA CTGAGTCCGG CTGAATACAG CGACAGTGAA
    GCTGAGGTTA CTATTAGCAC GCAACCCAAC AGAAAGGCAG AGAGGGGACA ACAGTTGCTG
    AGTGGGGAGG GACCAAAGAA TGAGGCCTTT ATAACCCCTT GCAAATCAGG AGGTGACCCA
    TCGCAAACAC ACTGTGCTCT AAACGCCACC ACGGCAGTCA ACAGAGTGAA AGTGCGCAGA
    ACAGAGGAAG AGTCGCCAAA GCCGGAGGAA TCAGAATCTA TTATAGGCCG CGTTCTAAAT
    CCCCAGGGGA AGGCAAATAA GAGAATCTCA TCAAAAGGAA AGAAAACCCG TAGCGCAACC
    CCCTCTCACA CCAAGGATCT GAGTGCTTGC AGGGAATCCC AGCACGAACT GACAGCCAGC
    AACCAGTCCA GCCTGGGCCT CCTGAACTCT ATGATCCAGG AAGTAGAGCA GGATCTGGCC
    GAGTATGAGA GGCAGACAGG ACGGGAAGTG ATATCCGCAC CCCCAGCTCA TGGTCTGACA
    GGATTCACTC TCTCTCTTGT CAGCTCGTTG AAACGAGTGG TGTCGTACCT GAAGGAGAGT
    GACCTGCTGC TGCAGCGAGA GGTGAAGGAG AGGCAGCACC TGCAGGGGGA GCTAGTGGAA
    CAGAGGCTGA TGCTCGATGC TCTGACTGCA GAAATTCTCA CCCTGAAAGA AGGAAACAAC
    ATCCATGAGA ATCACTTACA GACCAAACAG CGCCCAGAAG TAGGAGGGGA CAAGATGGCT
    GCCATCCCCC AAGAAATGGA AGAGCTTCCT GTAGTCAGTG AAGAACCTTG CAGAACCTCA
    GCTGAGTGCA ACCTTTCCAA GGTCAATCAA TTCCTGGATT CGCAGGATGG GCCAGATACA
    GATGATAAAC ACGGCAGACT TGGGGTGTCC AAGGAAGAAT GTGGCCCCTG TTTGTACCCC
    CAGGGCAGAG CTGCGGAGGA GCCCAGATTA GCCAGCTCTG TACCCTCACG TATGTTCCAG
    CAAGCTGTCT TGTTATCCCC TCCCAGGCAG AAAACTGTCA GTGAACTCAG CAGCCAATCA
    GCAGTTCCCA AGAGTTACAA ACATAGTAAC CGCCCAGCCC AGGGGGGCCA ACAAGTCAGC
    CATCATCCTA CTGCATCTAC AGAAAAGGAA CCCCCCAGCC AAGGACTAGA AATGGGTCAA
    AACCAAATAA ATCGCCTTCA AAACGAGGAC CTTGTTTCCC ATATGCAGCA GCTGGCGCTT
    CAGAATGCAG CACTTAAGGC TCAGCTTGGG CAAATACACT TTCCTCCAGC AGGAAATGCC
    CCAGAAGGGG GCAAATCTGT GCCAGCCGAA GAGAACCGTA ATCCAGAACC TGCTACGGAT
    CCACCGCAGA ACAAAGCAGC TCAGGTCCCA GGGAGCCTGG AGATGCGCAT CGCAGAGTTA
    AACAGGCAGA GTGCAGAAGC GCGGAACAAG CTCCTAAAAC TTATTGAGCA GCAGAAGCAG
    AGCATTGTGG TTTCTCCCAC ATTATCTCCC ATAACCCCCC AGGACAGGCG CACAGATTCC
    TCCCTGGATA CGACCCCCCC TTCCTCCTGC AGGACCTCGG GCACAAGGTT CTCCAGTGCA
    TCTAACAAGA GCACCTCTTC CATCAACTCG TCTTTTGGGA GTGTGAGATC GGCCTCTGCT
    GGGCGGAGGT CCCAGGTACG GTATAAACCT GTATTGCTGA GCTGTAGTAC GGTGTCTGCA
    CTGGGCCAAA ATCAAGCAGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32203
    Clone ID Related Accession (Same CDS sequence) XM_012956516.1
    Accession Version XM_012956516.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spindle and centriole-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGATCACT CTTCAGGTTC ACTTTCAATG TCTTATTTAA GAGCCGGCAG GACTTCAACG 
    AATGTTTCAC TCACAAAGAA GCCATCGAAG GGCAAAAAGA AACCGCAGGC CAGAAAGGAA
    TGGGATAGTA CAGTCAGTGA TCTCACGGTG CTCCGTGCCA CAAATGAAGA GCTGGAGCAC
    CGGCGGGAGA TACACAGATC CAAGAACCAG TGGCTGGCTC GTTGGGAACT CCAGAACAAA
    GCGACAAATA AAAAGTCGGA GAAAGAAAAT GGAGGATATT CAGCTCTTTT GGAAAATTCA
    CGCCTAGCTC TAATGAAGGA GATCCTTTCT GATCAGTATT TGATGAATGA TGTATTGGAG
    AGGTCAGACC GAGCCCTTGC TGTAGTGAAG GACCTGTTTG GAGATGCTCC TCGCAGACAC
    ACAGGATTTC CCAGTGTGAC TATGGCTCCT TCCTGTGATC TGGACACATC CCGAGCACCC
    ATAGTGCAGA AGAAGGATCC CCCAACCCAG CTTTCTATTC TGAGCGAGTC AATCATGGAT
    TCCCAGGCTA TCAATGAAGT GGACAAATCA CTGAGTCCGG CTGAATACAG CGACAGTGAA
    GCTGAGGTTA CTATTAGCAC GCAACCCAAC AGAAAGGCAG AGAGGGGACA ACAGTTGCTG
    AGTGGGGAGG GACCAAAGAA TGAGGCCTTT ATAACCCCTT GCAAATCAGG AGGTGACCCA
    TCGCAAACAC ACTGTGCTCT AAACGCCACC ACGGCAGTCA ACAGAGTGAA AGTGCGCAGA
    ACAGAGGAAG AGTCGCCAAA GCCGGAGGAA TCAGAATCTA TTATAGGCCG CGTTCTAAAT
    CCCCAGGGGA AGGCAAATAA GAGAATCTCA TCAAAAGGAA AGAAAACCCG TAGCGCAACC
    CCCTCTCACA CCAAGGATCT GAGTGCTTGC AGGGAATCCC AGCACGAACT GACAGCCAGC
    AACCAGTCCA GCCTGGGCCT CCTGAACTCT ATGATCCAGG AAGTAGAGCA GGATCTGGCC
    GAGTATGAGA GGCAGACAGG ACGGGAAGTG ATATCCGCAC CCCCAGCTCA TGGTCTGACA
    GGATTCACTC TCTCTCTTGT CAGCTCGTTG AAACGAGTGG TGTCGTACCT GAAGGAGAGT
    GACCTGCTGC TGCAGCGAGA GGTGAAGGAG AGGCAGCACC TGCAGGGGGA GCTAGTGGAA
    CAGAGGCTGA TGCTCGATGC TCTGACTGCA GAAATTCTCA CCCTGAAAGA AGGAAACAAC
    ATCCATGAGA ATCACTTACA GACCAAACAG CGCCCAGAAG TAGGAGGGGA CAAGATGGCT
    GCCATCCCCC AAGAAATGGA AGAGCTTCCT GTAGTCAGTG AAGAACCTTG CAGAACCTCA
    GCTGAGTGCA ACCTTTCCAA GGTCAATCAA TTCCTGGATT CGCAGGATGG GCCAGATACA
    GATGATAAAC ACGGCAGACT TGGGGTGTCC AAGGAAGAAT GTGGCCCCTG TTTGTACCCC
    CAGGGCAGAG CTGCGGAGGA GCCCAGATTA GCCAGCTCTG TACCCTCACG TATGTTCCAG
    CAAGCTGTCT TGTTATCCCC TCCCAGGCAG AAAACTGTCA GTGAACTCAG CAGCCAATCA
    GCAGTTCCCA AGAGTTACAA ACATAGTAAC CGCCCAGCCC AGGGGGGCCA ACAAGTCAGC
    CATCATCCTA CTGCATCTAC AGAAAAGGAA CCCCCCAGCC AAGGACTAGA AATGGGTCAA
    AACCAAATAA ATCGCCTTCA AAACGAGGAC CTTGTTTCCC ATATGCAGCA GCTGGCGCTT
    CAGAATGCAG CACTTAAGGC TCAGCTTGGG CAAATACACT TTCCTCCAGC AGGAAATGCC
    CCAGAAGGGG GCAAATCTGT GCCAGCCGAA GAGAACCGTA ATCCAGAACC TGCTACGGAT
    CCACCGCAGA ACAAAGCAGC TCAGGTCCCA GGGAGCCTGG AGATGCGCAT CGCAGAGTTA
    AACAGGCAGA GTGCAGAAGC GCGGAACAAG CTCCTAAAAC TTATTGAGCA GCAGAAGCAG
    AGCATTGTGG TTTCTCCCAC ATTATCTCCC ATAACCCCCC AGGACAGGCG CACAGATTCC
    TCCCTGGATA CGACCCCCCC TTCCTCCTGC AGGACCTCGG GCACAAGGTT CTCCAGTGCA
    TCTAACAAGA GCACCTCTTC CATCAACTCG TCTTTTGGGA GTGTGAGATC GGCCTCTGCT
    GGGCGGAGGT CCCAGGCTGA CAGTGAGAGG GGCGAGGGCT GGTTTGCTCT TTCTGCCCAC
    GTCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811970.1
    CDS381..2669
    Translation

    Target ORF information:

    RefSeq Version XM_012956516.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956516.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGATCACT CTTCAGGTTC ACTTTCAATG TCTTATTTAA GAGCCGGCAG GACTTCAACG 
    AATGTTTCAC TCACAAAGAA GCCATCGAAG GGCAAAAAGA AACCGCAGGC CAGAAAGGAA
    TGGGATAGTA CAGTCAGTGA TCTCACGGTG CTCCGTGCCA CAAATGAAGA GCTGGAGCAC
    CGGCGGGAGA TACACAGATC CAAGAACCAG TGGCTGGCTC GTTGGGAACT CCAGAACAAA
    GCGACAAATA AAAAGTCGGA GAAAGAAAAT GGAGGATATT CAGCTCTTTT GGAAAATTCA
    CGCCTAGCTC TAATGAAGGA GATCCTTTCT GATCAGTATT TGATGAATGA TGTATTGGAG
    AGGTCAGACC GAGCCCTTGC TGTAGTGAAG GACCTGTTTG GAGATGCTCC TCGCAGACAC
    ACAGGATTTC CCAGTGTGAC TATGGCTCCT TCCTGTGATC TGGACACATC CCGAGCACCC
    ATAGTGCAGA AGAAGGATCC CCCAACCCAG CTTTCTATTC TGAGCGAGTC AATCATGGAT
    TCCCAGGCTA TCAATGAAGT GGACAAATCA CTGAGTCCGG CTGAATACAG CGACAGTGAA
    GCTGAGGTTA CTATTAGCAC GCAACCCAAC AGAAAGGCAG AGAGGGGACA ACAGTTGCTG
    AGTGGGGAGG GACCAAAGAA TGAGGCCTTT ATAACCCCTT GCAAATCAGG AGGTGACCCA
    TCGCAAACAC ACTGTGCTCT AAACGCCACC ACGGCAGTCA ACAGAGTGAA AGTGCGCAGA
    ACAGAGGAAG AGTCGCCAAA GCCGGAGGAA TCAGAATCTA TTATAGGCCG CGTTCTAAAT
    CCCCAGGGGA AGGCAAATAA GAGAATCTCA TCAAAAGGAA AGAAAACCCG TAGCGCAACC
    CCCTCTCACA CCAAGGATCT GAGTGCTTGC AGGGAATCCC AGCACGAACT GACAGCCAGC
    AACCAGTCCA GCCTGGGCCT CCTGAACTCT ATGATCCAGG AAGTAGAGCA GGATCTGGCC
    GAGTATGAGA GGCAGACAGG ACGGGAAGTG ATATCCGCAC CCCCAGCTCA TGGTCTGACA
    GGATTCACTC TCTCTCTTGT CAGCTCGTTG AAACGAGTGG TGTCGTACCT GAAGGAGAGT
    GACCTGCTGC TGCAGCGAGA GGTGAAGGAG AGGCAGCACC TGCAGGGGGA GCTAGTGGAA
    CAGAGGCTGA TGCTCGATGC TCTGACTGCA GAAATTCTCA CCCTGAAAGA AGGAAACAAC
    ATCCATGAGA ATCACTTACA GACCAAACAG CGCCCAGAAG TAGGAGGGGA CAAGATGGCT
    GCCATCCCCC AAGAAATGGA AGAGCTTCCT GTAGTCAGTG AAGAACCTTG CAGAACCTCA
    GCTGAGTGCA ACCTTTCCAA GGTCAATCAA TTCCTGGATT CGCAGGATGG GCCAGATACA
    GATGATAAAC ACGGCAGACT TGGGGTGTCC AAGGAAGAAT GTGGCCCCTG TTTGTACCCC
    CAGGGCAGAG CTGCGGAGGA GCCCAGATTA GCCAGCTCTG TACCCTCACG TATGTTCCAG
    CAAGCTGTCT TGTTATCCCC TCCCAGGCAG AAAACTGTCA GTGAACTCAG CAGCCAATCA
    GCAGTTCCCA AGAGTTACAA ACATAGTAAC CGCCCAGCCC AGGGGGGCCA ACAAGTCAGC
    CATCATCCTA CTGCATCTAC AGAAAAGGAA CCCCCCAGCC AAGGACTAGA AATGGGTCAA
    AACCAAATAA ATCGCCTTCA AAACGAGGAC CTTGTTTCCC ATATGCAGCA GCTGGCGCTT
    CAGAATGCAG CACTTAAGGC TCAGCTTGGG CAAATACACT TTCCTCCAGC AGGAAATGCC
    CCAGAAGGGG GCAAATCTGT GCCAGCCGAA GAGAACCGTA ATCCAGAACC TGCTACGGAT
    CCACCGCAGA ACAAAGCAGC TCAGGTCCCA GGGAGCCTGG AGATGCGCAT CGCAGAGTTA
    AACAGGCAGA GTGCAGAAGC GCGGAACAAG CTCCTAAAAC TTATTGAGCA GCAGAAGCAG
    AGCATTGTGG TTTCTCCCAC ATTATCTCCC ATAACCCCCC AGGACAGGCG CACAGATTCC
    TCCCTGGATA CGACCCCCCC TTCCTCCTGC AGGACCTCGG GCACAAGGTT CTCCAGTGCA
    TCTAACAAGA GCACCTCTTC CATCAACTCG TCTTTTGGGA GTGTGAGATC GGCCTCTGCT
    GGGCGGAGGT CCCAGGCTGA CAGTGAGAGG GGCGAGGGCT GGTTTGCTCT TTCTGCCCAC
    GTCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32204
    Clone ID Related Accession (Same CDS sequence) XM_012956517.1
    Accession Version XM_012956517.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spindle and centriole-associated protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGATCACT CTTCAGGTTC ACTTTCAATG TCTTATTTAA GAGCCGGCAG GACTTCAACG 
    AATGTTTCAC TCACAAAGAA GCCATCGAAG GGCAAAAAGA AACCGCAGGC CAGAAAGGAA
    TGGGATAGTA CAGTCAGTGA TCTCACGGTG CTCCGTGCCA CAAATGAAGA GCTGGAGCAC
    CGGCGGGAGA TACACAGATC CAAGAACCAG TGGCTGGCTC GTTGGGAACT CCAGAACAAA
    GCGACAAATA AAAAGTCGGA GAAAGAAAAT GGAGGATATT CAGCTCTTTT GGAAAATTCA
    CGCCTAGCTC TAATGAAGGA GATCCTTTCT GATCAGTATT TGATGAATGA TGTATTGGAG
    AGGTCAGACC GAGCCCTTGC TGTAGTGAAG GACCTGTTTG GAGATGCTCC TCGCAGACAC
    ACAGGATTTC CCAGTGTGAC TATGGCTCCT TCCTGTGATC TGGACACATC CCGAGCACCC
    ATAGTGCAGA AGAAGGATCC CCCAACCCAG CTTTCTATTC TGAGCGAGTC AATCATGGAT
    TCCCAGGCTA TCAATGAAGT GGACAAATCA CTGAGTCCGG CTGAATACAG CGACAGTGAA
    GCTGAGGTTA CTATTAGCAC GCAACCCAAC AGAAAGGCAG AGAGGGGACA ACAGTTGCTG
    AGTGGGGAGG GACCAAAGAA TGAGGCCTTT ATAACCCCTT GCAAATCAGG AGGTGACCCA
    TCGCAAACAC ACTGTGCTCT AAACGCCACC ACGGCAGTCA ACAGAGTGAA AGTGCGCAGA
    ACAGAGGAAG AGTCGCCAAA GCCGGAGGAA TCAGAATCTA TTATAGGCCG CGTTCTAAAT
    CCCCAGGGGA AGGCAAATAA GAGAATCTCA TCAAAAGGAA AGAAAACCCG TAGCGCAACC
    CCCTCTCACA CCAAGGATCT GAGTGCTTGC AGGGAATCCC AGCACGAACT GACAGCCAGC
    AACCAGTCCA GCCTGGGCCT CCTGAACTCT ATGATCCAGG AAGTAGAGCA GGATCTGGCC
    GAGTATGAGA GGCAGACAGG ACGGGAAGTG ATATCCGCAC CCCCAGCTCA TGGTCTGACA
    GGATTCACTC TCTCTCTTGT CAGCTCGTTG AAACGAGTGG TGTCGTACCT GAAGGAGAGT
    GACCTGCTGC TGCAGCGAGA GGTGAAGGAG AGGCAGCACC TGCAGGGGGA GCTAGTGGAA
    CAGAGGCTGA TGCTCGATGC TCTGACTGCA GAAATTCTCA CCCTGAAAGA AGGAAACAAC
    ATCCATGAGA ATCACTTACA GACCAAACAG CGCCCAGAAG TAGGAGGGGA CAAGATGGCT
    GCCATCCCCC AAGAAATGGA AGAGCTTCCT GTAGTCAGTG AAGAACCTTG CAGAACCTCA
    GCTGAGTGCA ACCTTTCCAA GGTCAATCAA TTCCTGGATT CGCAGGATGG GCCAGATACA
    GATGATAAAC ACGGCAGACT TGGGGTGTCC AAGGAAGAAT GTGGCCCCTG TTTGTACCCC
    CAGGGCAGAG CTGCGGAGGA GCCCAGATTA GCCAGCTCTG TACCCTCACG TATGTTCCAG
    CAAGCTGTCT TGTTATCCCC TCCCAGGCAG AAAACTGTCA GTGAACTCAG CAGCCAATCA
    GCAGTTCCCA AGAGTTACAA ACATAGTAAC CGCCCAGCCC AGGGGGGCCA ACAAGTCAGC
    CATCATCCTA CTGCATCTAC AGAAAAGGAA CCCCCCAGCC AAGGACTAGA AATGGGTCAA
    AACCAAATAA ATCGCCTTCA AAACGAGGAC CTTGTTTCCC ATATGCAGCA GCTGGCGCTT
    CAGAATGCAG CACTTAAGGC TCAGCTTGGG CAAATACACT TTCCTCCAGC AGGAAATGCC
    CCAGAAGGGG GCAAATCTGT GCCAGCCGAA GAGAACCGTA ATCCAGAACC TGCTACGGAT
    CCACCGAACA AAGCAGCTCA GGTCCCAGGG AGCCTGGAGA TGCGCATCGC AGAGTTAAAC
    AGGCAGAGTG CAGAAGCGCG GAACAAGCTC CTAAAACTTA TTGAGCAGCA GAAGCAGAGC
    ATTGTGGTTT CTCCCACATT ATCTCCCATA ACCCCCCAGG ACAGGCGCAC AGATTCCTCC
    CTGGATACGA CCCCCCCTTC CTCCTGCAGG ACCTCGGGCA CAAGGTTCTC CAGTGCATCT
    AACAAGAGCA CCTCTTCCAT CAACTCGTCT TTTGGGAGTG TGAGATCGGC CTCTGCTGGG
    CGGAGGTCCC AGGCTGACAG TGAGAGGGGC GAGGGCTGGT TTGCTCTTTC TGCCCACGTC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811971.1
    CDS381..2666
    Translation

    Target ORF information:

    RefSeq Version XM_012956517.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956517.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGATCACT CTTCAGGTTC ACTTTCAATG TCTTATTTAA GAGCCGGCAG GACTTCAACG 
    AATGTTTCAC TCACAAAGAA GCCATCGAAG GGCAAAAAGA AACCGCAGGC CAGAAAGGAA
    TGGGATAGTA CAGTCAGTGA TCTCACGGTG CTCCGTGCCA CAAATGAAGA GCTGGAGCAC
    CGGCGGGAGA TACACAGATC CAAGAACCAG TGGCTGGCTC GTTGGGAACT CCAGAACAAA
    GCGACAAATA AAAAGTCGGA GAAAGAAAAT GGAGGATATT CAGCTCTTTT GGAAAATTCA
    CGCCTAGCTC TAATGAAGGA GATCCTTTCT GATCAGTATT TGATGAATGA TGTATTGGAG
    AGGTCAGACC GAGCCCTTGC TGTAGTGAAG GACCTGTTTG GAGATGCTCC TCGCAGACAC
    ACAGGATTTC CCAGTGTGAC TATGGCTCCT TCCTGTGATC TGGACACATC CCGAGCACCC
    ATAGTGCAGA AGAAGGATCC CCCAACCCAG CTTTCTATTC TGAGCGAGTC AATCATGGAT
    TCCCAGGCTA TCAATGAAGT GGACAAATCA CTGAGTCCGG CTGAATACAG CGACAGTGAA
    GCTGAGGTTA CTATTAGCAC GCAACCCAAC AGAAAGGCAG AGAGGGGACA ACAGTTGCTG
    AGTGGGGAGG GACCAAAGAA TGAGGCCTTT ATAACCCCTT GCAAATCAGG AGGTGACCCA
    TCGCAAACAC ACTGTGCTCT AAACGCCACC ACGGCAGTCA ACAGAGTGAA AGTGCGCAGA
    ACAGAGGAAG AGTCGCCAAA GCCGGAGGAA TCAGAATCTA TTATAGGCCG CGTTCTAAAT
    CCCCAGGGGA AGGCAAATAA GAGAATCTCA TCAAAAGGAA AGAAAACCCG TAGCGCAACC
    CCCTCTCACA CCAAGGATCT GAGTGCTTGC AGGGAATCCC AGCACGAACT GACAGCCAGC
    AACCAGTCCA GCCTGGGCCT CCTGAACTCT ATGATCCAGG AAGTAGAGCA GGATCTGGCC
    GAGTATGAGA GGCAGACAGG ACGGGAAGTG ATATCCGCAC CCCCAGCTCA TGGTCTGACA
    GGATTCACTC TCTCTCTTGT CAGCTCGTTG AAACGAGTGG TGTCGTACCT GAAGGAGAGT
    GACCTGCTGC TGCAGCGAGA GGTGAAGGAG AGGCAGCACC TGCAGGGGGA GCTAGTGGAA
    CAGAGGCTGA TGCTCGATGC TCTGACTGCA GAAATTCTCA CCCTGAAAGA AGGAAACAAC
    ATCCATGAGA ATCACTTACA GACCAAACAG CGCCCAGAAG TAGGAGGGGA CAAGATGGCT
    GCCATCCCCC AAGAAATGGA AGAGCTTCCT GTAGTCAGTG AAGAACCTTG CAGAACCTCA
    GCTGAGTGCA ACCTTTCCAA GGTCAATCAA TTCCTGGATT CGCAGGATGG GCCAGATACA
    GATGATAAAC ACGGCAGACT TGGGGTGTCC AAGGAAGAAT GTGGCCCCTG TTTGTACCCC
    CAGGGCAGAG CTGCGGAGGA GCCCAGATTA GCCAGCTCTG TACCCTCACG TATGTTCCAG
    CAAGCTGTCT TGTTATCCCC TCCCAGGCAG AAAACTGTCA GTGAACTCAG CAGCCAATCA
    GCAGTTCCCA AGAGTTACAA ACATAGTAAC CGCCCAGCCC AGGGGGGCCA ACAAGTCAGC
    CATCATCCTA CTGCATCTAC AGAAAAGGAA CCCCCCAGCC AAGGACTAGA AATGGGTCAA
    AACCAAATAA ATCGCCTTCA AAACGAGGAC CTTGTTTCCC ATATGCAGCA GCTGGCGCTT
    CAGAATGCAG CACTTAAGGC TCAGCTTGGG CAAATACACT TTCCTCCAGC AGGAAATGCC
    CCAGAAGGGG GCAAATCTGT GCCAGCCGAA GAGAACCGTA ATCCAGAACC TGCTACGGAT
    CCACCGAACA AAGCAGCTCA GGTCCCAGGG AGCCTGGAGA TGCGCATCGC AGAGTTAAAC
    AGGCAGAGTG CAGAAGCGCG GAACAAGCTC CTAAAACTTA TTGAGCAGCA GAAGCAGAGC
    ATTGTGGTTT CTCCCACATT ATCTCCCATA ACCCCCCAGG ACAGGCGCAC AGATTCCTCC
    CTGGATACGA CCCCCCCTTC CTCCTGCAGG ACCTCGGGCA CAAGGTTCTC CAGTGCATCT
    AACAAGAGCA CCTCTTCCAT CAACTCGTCT TTTGGGAGTG TGAGATCGGC CTCTGCTGGG
    CGGAGGTCCC AGGCTGACAG TGAGAGGGGC GAGGGCTGGT TTGCTCTTTC TGCCCACGTC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32205
    Clone ID Related Accession (Same CDS sequence) XM_012956518.2 , XM_031895713.1 , XM_031895712.1 , XM_012956518.3
    Accession Version XM_012956518.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spindle and centriole-associated protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTTATT TAAGAGCCGG CAGGACTTCA ACGAATGTTT CACTCACAAA GAAGCCATCG 
    AAGGGCAAAA AGAAACCGCA GGCCAGAAAG GAATGGGATA GTACAGTCAG TGATCTCACG
    GTGCTCCGTG CCACAAATGA AGAGCTGGAG CACCGGCGGG AGATACACAG ATCCAAGAAC
    CAGTGGCTGG CTCGTTGGGA ACTCCAGAAC AAAGCGACAA ATAAAAAGTC GGAGAAAGAA
    AATGGAGGAT ATTCAGCTCT TTTGGAAAAT TCACGCCTAG CTCTAATGAA GGAGATCCTT
    TCTGATCAGT ATTTGATGAA TGATGTATTG GAGAGGTCAG ACCGAGCCCT TGCTGTAGTG
    AAGGACCTGT TTGGAGATGC TCCTCGCAGA CACACAGGAT TTCCCAGTGT GACTATGGCT
    CCTTCCTGTG ATCTGGACAC ATCCCGAGCA CCCATAGTGC AGAAGAAGGA TCCCCCAACC
    CAGCTTTCTA TTCTGAGCGA GTCAATCATG GATTCCCAGG CTATCAATGA AGTGGACAAA
    TCACTGAGTC CGGCTGAATA CAGCGACAGT GAAGCTGAGG TTACTATTAG CACGCAACCC
    AACAGAAAGG CAGAGAGGGG ACAACAGTTG CTGAGTGGGG AGGGACCAAA GAATGAGGCC
    TTTATAACCC CTTGCAAATC AGGAGGTGAC CCATCGCAAA CACACTGTGC TCTAAACGCC
    ACCACGGCAG TCAACAGAGT GAAAGTGCGC AGAACAGAGG AAGAGTCGCC AAAGCCGGAG
    GAATCAGAAT CTATTATAGG CCGCGTTCTA AATCCCCAGG GGAAGGCAAA TAAGAGAATC
    TCATCAAAAG GAAAGAAAAC CCGTAGCGCA ACCCCCTCTC ACACCAAGGA TCTGAGTGCT
    TGCAGGGAAT CCCAGCACGA ACTGACAGCC AGCAACCAGT CCAGCCTGGG CCTCCTGAAC
    TCTATGATCC AGGAAGTAGA GCAGGATCTG GCCGAGTATG AGAGGCAGAC AGGACGGGAA
    GTGATATCCG CACCCCCAGC TCATGGTCTG ACAGGATTCA CTCTCTCTCT TGTCAGCTCG
    TTGAAACGAG TGGTGTCGTA CCTGAAGGAG AGTGACCTGC TGCTGCAGCG AGAGGTGAAG
    GAGAGGCAGC ACCTGCAGGG GGAGCTAGTG GAACAGAGGC TGATGCTCGA TGCTCTGACT
    GCAGAAATTC TCACCCTGAA AGAAGGAAAC AACATCCATG AGAATCACTT ACAGACCAAA
    CAGCGCCCAG AAGTAGGAGG GGACAAGATG GCTGCCATCC CCCAAGAAAT GGAAGAGCTT
    CCTGTAGTCA GTGAAGAACC TTGCAGAACC TCAGCTGAGT GCAACCTTTC CAAGGTCAAT
    CAATTCCTGG ATTCGCAGGA TGGGCCAGAT ACAGATGATA AACACGGCAG ACTTGGGGTG
    TCCAAGGAAG AATGTGGCCC CTGTTTGTAC CCCCAGGGCA GAGCTGCGGA GGAGCCCAGA
    TTAGCCAGCT CTGTACCCTC ACGTATGTTC CAGCAAGCTG TCTTGTTATC CCCTCCCAGG
    CAGAAAACTG TCAGTGAACT CAGCAGCCAA TCAGCAGTTC CCAAGAGTTA CAAACATAGT
    AACCGCCCAG CCCAGGGGGG CCAACAAGTC AGCCATCATC CTACTGCATC TACAGAAAAG
    GAACCCCCCA GCCAAGGACT AGAAATGGGT CAAAACCAAA TAAATCGCCT TCAAAACGAG
    GACCTTGTTT CCCATATGCA GCAGCTGGCG CTTCAGAATG CAGCACTTAA GGCTCAGCTT
    GGGCAAATAC ACTTTCCTCC AGCAGGAAAT GCCCCAGAAG GGGGCAAATC TGTGCCAGCC
    GAAGAGAACC GTAATCCAGA ACCTGCTACG GATCCACCGC AGAACAAAGC AGCTCAGGTC
    CCAGGGAGCC TGGAGATGCG CATCGCAGAG TTAAACAGGC AGAGTGCAGA AGCGCGGAAC
    AAGCTCCTAA AACTTATTGA GCAGCAGAAG CAGAGCATTG TGGTTTCTCC CACATTATCT
    CCCATAACCC CCCAGGACAG GCGCACAGAT TCCTCCCTGG ATACGACCCC CCCTTCCTCC
    TGCAGGACCT CGGGCACAAG GTTCTCCAGT GCATCTAACA AGAGCACCTC TTCCATCAAC
    TCGTCTTTTG GGAGTGTGAG ATCGGCCTCT GCTGGGCGGA GGTCCCAGGT ACGGTATAAA
    CCTGTATTGC TGAGCTGTAG TACGGTGTCT GCACTGGGCC AAAATCAAGC AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811972.1
    CDS369..2645
    Translation

    Target ORF information:

    RefSeq Version XM_012956518.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956518.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTTATT TAAGAGCCGG CAGGACTTCA ACGAATGTTT CACTCACAAA GAAGCCATCG 
    AAGGGCAAAA AGAAACCGCA GGCCAGAAAG GAATGGGATA GTACAGTCAG TGATCTCACG
    GTGCTCCGTG CCACAAATGA AGAGCTGGAG CACCGGCGGG AGATACACAG ATCCAAGAAC
    CAGTGGCTGG CTCGTTGGGA ACTCCAGAAC AAAGCGACAA ATAAAAAGTC GGAGAAAGAA
    AATGGAGGAT ATTCAGCTCT TTTGGAAAAT TCACGCCTAG CTCTAATGAA GGAGATCCTT
    TCTGATCAGT ATTTGATGAA TGATGTATTG GAGAGGTCAG ACCGAGCCCT TGCTGTAGTG
    AAGGACCTGT TTGGAGATGC TCCTCGCAGA CACACAGGAT TTCCCAGTGT GACTATGGCT
    CCTTCCTGTG ATCTGGACAC ATCCCGAGCA CCCATAGTGC AGAAGAAGGA TCCCCCAACC
    CAGCTTTCTA TTCTGAGCGA GTCAATCATG GATTCCCAGG CTATCAATGA AGTGGACAAA
    TCACTGAGTC CGGCTGAATA CAGCGACAGT GAAGCTGAGG TTACTATTAG CACGCAACCC
    AACAGAAAGG CAGAGAGGGG ACAACAGTTG CTGAGTGGGG AGGGACCAAA GAATGAGGCC
    TTTATAACCC CTTGCAAATC AGGAGGTGAC CCATCGCAAA CACACTGTGC TCTAAACGCC
    ACCACGGCAG TCAACAGAGT GAAAGTGCGC AGAACAGAGG AAGAGTCGCC AAAGCCGGAG
    GAATCAGAAT CTATTATAGG CCGCGTTCTA AATCCCCAGG GGAAGGCAAA TAAGAGAATC
    TCATCAAAAG GAAAGAAAAC CCGTAGCGCA ACCCCCTCTC ACACCAAGGA TCTGAGTGCT
    TGCAGGGAAT CCCAGCACGA ACTGACAGCC AGCAACCAGT CCAGCCTGGG CCTCCTGAAC
    TCTATGATCC AGGAAGTAGA GCAGGATCTG GCCGAGTATG AGAGGCAGAC AGGACGGGAA
    GTGATATCCG CACCCCCAGC TCATGGTCTG ACAGGATTCA CTCTCTCTCT TGTCAGCTCG
    TTGAAACGAG TGGTGTCGTA CCTGAAGGAG AGTGACCTGC TGCTGCAGCG AGAGGTGAAG
    GAGAGGCAGC ACCTGCAGGG GGAGCTAGTG GAACAGAGGC TGATGCTCGA TGCTCTGACT
    GCAGAAATTC TCACCCTGAA AGAAGGAAAC AACATCCATG AGAATCACTT ACAGACCAAA
    CAGCGCCCAG AAGTAGGAGG GGACAAGATG GCTGCCATCC CCCAAGAAAT GGAAGAGCTT
    CCTGTAGTCA GTGAAGAACC TTGCAGAACC TCAGCTGAGT GCAACCTTTC CAAGGTCAAT
    CAATTCCTGG ATTCGCAGGA TGGGCCAGAT ACAGATGATA AACACGGCAG ACTTGGGGTG
    TCCAAGGAAG AATGTGGCCC CTGTTTGTAC CCCCAGGGCA GAGCTGCGGA GGAGCCCAGA
    TTAGCCAGCT CTGTACCCTC ACGTATGTTC CAGCAAGCTG TCTTGTTATC CCCTCCCAGG
    CAGAAAACTG TCAGTGAACT CAGCAGCCAA TCAGCAGTTC CCAAGAGTTA CAAACATAGT
    AACCGCCCAG CCCAGGGGGG CCAACAAGTC AGCCATCATC CTACTGCATC TACAGAAAAG
    GAACCCCCCA GCCAAGGACT AGAAATGGGT CAAAACCAAA TAAATCGCCT TCAAAACGAG
    GACCTTGTTT CCCATATGCA GCAGCTGGCG CTTCAGAATG CAGCACTTAA GGCTCAGCTT
    GGGCAAATAC ACTTTCCTCC AGCAGGAAAT GCCCCAGAAG GGGGCAAATC TGTGCCAGCC
    GAAGAGAACC GTAATCCAGA ACCTGCTACG GATCCACCGC AGAACAAAGC AGCTCAGGTC
    CCAGGGAGCC TGGAGATGCG CATCGCAGAG TTAAACAGGC AGAGTGCAGA AGCGCGGAAC
    AAGCTCCTAA AACTTATTGA GCAGCAGAAG CAGAGCATTG TGGTTTCTCC CACATTATCT
    CCCATAACCC CCCAGGACAG GCGCACAGAT TCCTCCCTGG ATACGACCCC CCCTTCCTCC
    TGCAGGACCT CGGGCACAAG GTTCTCCAGT GCATCTAACA AGAGCACCTC TTCCATCAAC
    TCGTCTTTTG GGAGTGTGAG ATCGGCCTCT GCTGGGCGGA GGTCCCAGGT ACGGTATAAA
    CCTGTATTGC TGAGCTGTAG TACGGTGTCT GCACTGGGCC AAAATCAAGC AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32202
    Clone ID Related Accession (Same CDS sequence) XM_012956515.3 , XM_012956515.2
    Accession Version XM_012956515.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spindle and centriole-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956515.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGATCACT CTTCAGGTTC ACTTTCAATG TCTTATTTAA GAGCCGGCAG GACTTCAACG 
    AATGTTTCAC TCACAAAGAA GCCATCGAAG GGCAAAAAGA AACCGCAGGC CAGAAAGGAA
    TGGGATAGTA CAGTCAGTGA TCTCACGGTG CTCCGTGCCA CAAATGAAGA GCTGGAGCAC
    CGGCGGGAGA TACACAGATC CAAGAACCAG TGGCTGGCTC GTTGGGAACT CCAGAACAAA
    GCGACAAATA AAAAGTCGGA GAAAGAAAAT GGAGGATATT CAGCTCTTTT GGAAAATTCA
    CGCCTAGCTC TAATGAAGGA GATCCTTTCT GATCAGTATT TGATGAATGA TGTATTGGAG
    AGGTCAGACC GAGCCCTTGC TGTAGTGAAG GACCTGTTTG GAGATGCTCC TCGCAGACAC
    ACAGGATTTC CCAGTGTGAC TATGGCTCCT TCCTGTGATC TGGACACATC CCGAGCACCC
    ATAGTGCAGA AGAAGGATCC CCCAACCCAG CTTTCTATTC TGAGCGAGTC AATCATGGAT
    TCCCAGGCTA TCAATGAAGT GGACAAATCA CTGAGTCCGG CTGAATACAG CGACAGTGAA
    GCTGAGGTTA CTATTAGCAC GCAACCCAAC AGAAAGGCAG AGAGGGGACA ACAGTTGCTG
    AGTGGGGAGG GACCAAAGAA TGAGGCCTTT ATAACCCCTT GCAAATCAGG AGGTGACCCA
    TCGCAAACAC ACTGTGCTCT AAACGCCACC ACGGCAGTCA ACAGAGTGAA AGTGCGCAGA
    ACAGAGGAAG AGTCGCCAAA GCCGGAGGAA TCAGAATCTA TTATAGGCCG CGTTCTAAAT
    CCCCAGGGGA AGGCAAATAA GAGAATCTCA TCAAAAGGAA AGAAAACCCG TAGCGCAACC
    CCCTCTCACA CCAAGGATCT GAGTGCTTGC AGGGAATCCC AGCACGAACT GACAGCCAGC
    AACCAGTCCA GCCTGGGCCT CCTGAACTCT ATGATCCAGG AAGTAGAGCA GGATCTGGCC
    GAGTATGAGA GGCAGACAGG ACGGGAAGTG ATATCCGCAC CCCCAGCTCA TGGTCTGACA
    GGATTCACTC TCTCTCTTGT CAGCTCGTTG AAACGAGTGG TGTCGTACCT GAAGGAGAGT
    GACCTGCTGC TGCAGCGAGA GGTGAAGGAG AGGCAGCACC TGCAGGGGGA GCTAGTGGAA
    CAGAGGCTGA TGCTCGATGC TCTGACTGCA GAAATTCTCA CCCTGAAAGA AGGAAACAAC
    ATCCATGAGA ATCACTTACA GACCAAACAG CGCCCAGAAG TAGGAGGGGA CAAGATGGCT
    GCCATCCCCC AAGAAATGGA AGAGCTTCCT GTAGTCAGTG AAGAACCTTG CAGAACCTCA
    GCTGAGTGCA ACCTTTCCAA GGTCAATCAA TTCCTGGATT CGCAGGATGG GCCAGATACA
    GATGATAAAC ACGGCAGACT TGGGGTGTCC AAGGAAGAAT GTGGCCCCTG TTTGTACCCC
    CAGGGCAGAG CTGCGGAGGA GCCCAGATTA GCCAGCTCTG TACCCTCACG TATGTTCCAG
    CAAGCTGTCT TGTTATCCCC TCCCAGGCAG AAAACTGTCA GTGAACTCAG CAGCCAATCA
    GCAGTTCCCA AGAGTTACAA ACATAGTAAC CGCCCAGCCC AGGGGGGCCA ACAAGTCAGC
    CATCATCCTA CTGCATCTAC AGAAAAGGAA CCCCCCAGCC AAGGACTAGA AATGGGTCAA
    AACCAAATAA ATCGCCTTCA AAACGAGGAC CTTGTTTCCC ATATGCAGCA GCTGGCGCTT
    CAGAATGCAG CACTTAAGGC TCAGCTTGGG CAAATACACT TTCCTCCAGC AGGAAATGCC
    CCAGAAGGGG GCAAATCTGT GCCAGCCGAA GAGAACCGTA ATCCAGAACC TGCTACGGAT
    CCACCGCAGA ACAAAGCAGC TCAGGTCCCA GGGAGCCTGG AGATGCGCAT CGCAGAGTTA
    AACAGGCAGA GTGCAGAAGC GCGGAACAAG CTCCTAAAAC TTATTGAGCA GCAGAAGCAG
    AGCATTGTGG TTTCTCCCAC ATTATCTCCC ATAACCCCCC AGGACAGGCG CACAGATTCC
    TCCCTGGATA CGACCCCCCC TTCCTCCTGC AGGACCTCGG GCACAAGGTT CTCCAGTGCA
    TCTAACAAGA GCACCTCTTC CATCAACTCG TCTTTTGGGA GTGTGAGATC GGCCTCTGCT
    GGGCGGAGGT CCCAGGTACG GTATAAACCT GTATTGCTGA GCTGTAGTAC GGTGTCTGCA
    CTGGGCCAAA ATCAAGCAGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811969.1
    CDS381..2684
    Translation

    Target ORF information:

    RefSeq Version XM_012956515.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956515.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGATCACT CTTCAGGTTC ACTTTCAATG TCTTATTTAA GAGCCGGCAG GACTTCAACG 
    AATGTTTCAC TCACAAAGAA GCCATCGAAG GGCAAAAAGA AACCGCAGGC CAGAAAGGAA
    TGGGATAGTA CAGTCAGTGA TCTCACGGTG CTCCGTGCCA CAAATGAAGA GCTGGAGCAC
    CGGCGGGAGA TACACAGATC CAAGAACCAG TGGCTGGCTC GTTGGGAACT CCAGAACAAA
    GCGACAAATA AAAAGTCGGA GAAAGAAAAT GGAGGATATT CAGCTCTTTT GGAAAATTCA
    CGCCTAGCTC TAATGAAGGA GATCCTTTCT GATCAGTATT TGATGAATGA TGTATTGGAG
    AGGTCAGACC GAGCCCTTGC TGTAGTGAAG GACCTGTTTG GAGATGCTCC TCGCAGACAC
    ACAGGATTTC CCAGTGTGAC TATGGCTCCT TCCTGTGATC TGGACACATC CCGAGCACCC
    ATAGTGCAGA AGAAGGATCC CCCAACCCAG CTTTCTATTC TGAGCGAGTC AATCATGGAT
    TCCCAGGCTA TCAATGAAGT GGACAAATCA CTGAGTCCGG CTGAATACAG CGACAGTGAA
    GCTGAGGTTA CTATTAGCAC GCAACCCAAC AGAAAGGCAG AGAGGGGACA ACAGTTGCTG
    AGTGGGGAGG GACCAAAGAA TGAGGCCTTT ATAACCCCTT GCAAATCAGG AGGTGACCCA
    TCGCAAACAC ACTGTGCTCT AAACGCCACC ACGGCAGTCA ACAGAGTGAA AGTGCGCAGA
    ACAGAGGAAG AGTCGCCAAA GCCGGAGGAA TCAGAATCTA TTATAGGCCG CGTTCTAAAT
    CCCCAGGGGA AGGCAAATAA GAGAATCTCA TCAAAAGGAA AGAAAACCCG TAGCGCAACC
    CCCTCTCACA CCAAGGATCT GAGTGCTTGC AGGGAATCCC AGCACGAACT GACAGCCAGC
    AACCAGTCCA GCCTGGGCCT CCTGAACTCT ATGATCCAGG AAGTAGAGCA GGATCTGGCC
    GAGTATGAGA GGCAGACAGG ACGGGAAGTG ATATCCGCAC CCCCAGCTCA TGGTCTGACA
    GGATTCACTC TCTCTCTTGT CAGCTCGTTG AAACGAGTGG TGTCGTACCT GAAGGAGAGT
    GACCTGCTGC TGCAGCGAGA GGTGAAGGAG AGGCAGCACC TGCAGGGGGA GCTAGTGGAA
    CAGAGGCTGA TGCTCGATGC TCTGACTGCA GAAATTCTCA CCCTGAAAGA AGGAAACAAC
    ATCCATGAGA ATCACTTACA GACCAAACAG CGCCCAGAAG TAGGAGGGGA CAAGATGGCT
    GCCATCCCCC AAGAAATGGA AGAGCTTCCT GTAGTCAGTG AAGAACCTTG CAGAACCTCA
    GCTGAGTGCA ACCTTTCCAA GGTCAATCAA TTCCTGGATT CGCAGGATGG GCCAGATACA
    GATGATAAAC ACGGCAGACT TGGGGTGTCC AAGGAAGAAT GTGGCCCCTG TTTGTACCCC
    CAGGGCAGAG CTGCGGAGGA GCCCAGATTA GCCAGCTCTG TACCCTCACG TATGTTCCAG
    CAAGCTGTCT TGTTATCCCC TCCCAGGCAG AAAACTGTCA GTGAACTCAG CAGCCAATCA
    GCAGTTCCCA AGAGTTACAA ACATAGTAAC CGCCCAGCCC AGGGGGGCCA ACAAGTCAGC
    CATCATCCTA CTGCATCTAC AGAAAAGGAA CCCCCCAGCC AAGGACTAGA AATGGGTCAA
    AACCAAATAA ATCGCCTTCA AAACGAGGAC CTTGTTTCCC ATATGCAGCA GCTGGCGCTT
    CAGAATGCAG CACTTAAGGC TCAGCTTGGG CAAATACACT TTCCTCCAGC AGGAAATGCC
    CCAGAAGGGG GCAAATCTGT GCCAGCCGAA GAGAACCGTA ATCCAGAACC TGCTACGGAT
    CCACCGCAGA ACAAAGCAGC TCAGGTCCCA GGGAGCCTGG AGATGCGCAT CGCAGAGTTA
    AACAGGCAGA GTGCAGAAGC GCGGAACAAG CTCCTAAAAC TTATTGAGCA GCAGAAGCAG
    AGCATTGTGG TTTCTCCCAC ATTATCTCCC ATAACCCCCC AGGACAGGCG CACAGATTCC
    TCCCTGGATA CGACCCCCCC TTCCTCCTGC AGGACCTCGG GCACAAGGTT CTCCAGTGCA
    TCTAACAAGA GCACCTCTTC CATCAACTCG TCTTTTGGGA GTGTGAGATC GGCCTCTGCT
    GGGCGGAGGT CCCAGGTACG GTATAAACCT GTATTGCTGA GCTGTAGTAC GGTGTCTGCA
    CTGGGCCAAA ATCAAGCAGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32205
    Clone ID Related Accession (Same CDS sequence) XM_012956518.2 , XM_031895713.1 , XM_031895712.1 , XM_012956518.3
    Accession Version XM_031895713.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spindle and centriole-associated protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTTATT TAAGAGCCGG CAGGACTTCA ACGAATGTTT CACTCACAAA GAAGCCATCG 
    AAGGGCAAAA AGAAACCGCA GGCCAGAAAG GAATGGGATA GTACAGTCAG TGATCTCACG
    GTGCTCCGTG CCACAAATGA AGAGCTGGAG CACCGGCGGG AGATACACAG ATCCAAGAAC
    CAGTGGCTGG CTCGTTGGGA ACTCCAGAAC AAAGCGACAA ATAAAAAGTC GGAGAAAGAA
    AATGGAGGAT ATTCAGCTCT TTTGGAAAAT TCACGCCTAG CTCTAATGAA GGAGATCCTT
    TCTGATCAGT ATTTGATGAA TGATGTATTG GAGAGGTCAG ACCGAGCCCT TGCTGTAGTG
    AAGGACCTGT TTGGAGATGC TCCTCGCAGA CACACAGGAT TTCCCAGTGT GACTATGGCT
    CCTTCCTGTG ATCTGGACAC ATCCCGAGCA CCCATAGTGC AGAAGAAGGA TCCCCCAACC
    CAGCTTTCTA TTCTGAGCGA GTCAATCATG GATTCCCAGG CTATCAATGA AGTGGACAAA
    TCACTGAGTC CGGCTGAATA CAGCGACAGT GAAGCTGAGG TTACTATTAG CACGCAACCC
    AACAGAAAGG CAGAGAGGGG ACAACAGTTG CTGAGTGGGG AGGGACCAAA GAATGAGGCC
    TTTATAACCC CTTGCAAATC AGGAGGTGAC CCATCGCAAA CACACTGTGC TCTAAACGCC
    ACCACGGCAG TCAACAGAGT GAAAGTGCGC AGAACAGAGG AAGAGTCGCC AAAGCCGGAG
    GAATCAGAAT CTATTATAGG CCGCGTTCTA AATCCCCAGG GGAAGGCAAA TAAGAGAATC
    TCATCAAAAG GAAAGAAAAC CCGTAGCGCA ACCCCCTCTC ACACCAAGGA TCTGAGTGCT
    TGCAGGGAAT CCCAGCACGA ACTGACAGCC AGCAACCAGT CCAGCCTGGG CCTCCTGAAC
    TCTATGATCC AGGAAGTAGA GCAGGATCTG GCCGAGTATG AGAGGCAGAC AGGACGGGAA
    GTGATATCCG CACCCCCAGC TCATGGTCTG ACAGGATTCA CTCTCTCTCT TGTCAGCTCG
    TTGAAACGAG TGGTGTCGTA CCTGAAGGAG AGTGACCTGC TGCTGCAGCG AGAGGTGAAG
    GAGAGGCAGC ACCTGCAGGG GGAGCTAGTG GAACAGAGGC TGATGCTCGA TGCTCTGACT
    GCAGAAATTC TCACCCTGAA AGAAGGAAAC AACATCCATG AGAATCACTT ACAGACCAAA
    CAGCGCCCAG AAGTAGGAGG GGACAAGATG GCTGCCATCC CCCAAGAAAT GGAAGAGCTT
    CCTGTAGTCA GTGAAGAACC TTGCAGAACC TCAGCTGAGT GCAACCTTTC CAAGGTCAAT
    CAATTCCTGG ATTCGCAGGA TGGGCCAGAT ACAGATGATA AACACGGCAG ACTTGGGGTG
    TCCAAGGAAG AATGTGGCCC CTGTTTGTAC CCCCAGGGCA GAGCTGCGGA GGAGCCCAGA
    TTAGCCAGCT CTGTACCCTC ACGTATGTTC CAGCAAGCTG TCTTGTTATC CCCTCCCAGG
    CAGAAAACTG TCAGTGAACT CAGCAGCCAA TCAGCAGTTC CCAAGAGTTA CAAACATAGT
    AACCGCCCAG CCCAGGGGGG CCAACAAGTC AGCCATCATC CTACTGCATC TACAGAAAAG
    GAACCCCCCA GCCAAGGACT AGAAATGGGT CAAAACCAAA TAAATCGCCT TCAAAACGAG
    GACCTTGTTT CCCATATGCA GCAGCTGGCG CTTCAGAATG CAGCACTTAA GGCTCAGCTT
    GGGCAAATAC ACTTTCCTCC AGCAGGAAAT GCCCCAGAAG GGGGCAAATC TGTGCCAGCC
    GAAGAGAACC GTAATCCAGA ACCTGCTACG GATCCACCGC AGAACAAAGC AGCTCAGGTC
    CCAGGGAGCC TGGAGATGCG CATCGCAGAG TTAAACAGGC AGAGTGCAGA AGCGCGGAAC
    AAGCTCCTAA AACTTATTGA GCAGCAGAAG CAGAGCATTG TGGTTTCTCC CACATTATCT
    CCCATAACCC CCCAGGACAG GCGCACAGAT TCCTCCCTGG ATACGACCCC CCCTTCCTCC
    TGCAGGACCT CGGGCACAAG GTTCTCCAGT GCATCTAACA AGAGCACCTC TTCCATCAAC
    TCGTCTTTTG GGAGTGTGAG ATCGGCCTCT GCTGGGCGGA GGTCCCAGGT ACGGTATAAA
    CCTGTATTGC TGAGCTGTAG TACGGTGTCT GCACTGGGCC AAAATCAAGC AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751573.1
    CDS110..2386
    Translation

    Target ORF information:

    RefSeq Version XM_031895713.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895713.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTTATT TAAGAGCCGG CAGGACTTCA ACGAATGTTT CACTCACAAA GAAGCCATCG 
    AAGGGCAAAA AGAAACCGCA GGCCAGAAAG GAATGGGATA GTACAGTCAG TGATCTCACG
    GTGCTCCGTG CCACAAATGA AGAGCTGGAG CACCGGCGGG AGATACACAG ATCCAAGAAC
    CAGTGGCTGG CTCGTTGGGA ACTCCAGAAC AAAGCGACAA ATAAAAAGTC GGAGAAAGAA
    AATGGAGGAT ATTCAGCTCT TTTGGAAAAT TCACGCCTAG CTCTAATGAA GGAGATCCTT
    TCTGATCAGT ATTTGATGAA TGATGTATTG GAGAGGTCAG ACCGAGCCCT TGCTGTAGTG
    AAGGACCTGT TTGGAGATGC TCCTCGCAGA CACACAGGAT TTCCCAGTGT GACTATGGCT
    CCTTCCTGTG ATCTGGACAC ATCCCGAGCA CCCATAGTGC AGAAGAAGGA TCCCCCAACC
    CAGCTTTCTA TTCTGAGCGA GTCAATCATG GATTCCCAGG CTATCAATGA AGTGGACAAA
    TCACTGAGTC CGGCTGAATA CAGCGACAGT GAAGCTGAGG TTACTATTAG CACGCAACCC
    AACAGAAAGG CAGAGAGGGG ACAACAGTTG CTGAGTGGGG AGGGACCAAA GAATGAGGCC
    TTTATAACCC CTTGCAAATC AGGAGGTGAC CCATCGCAAA CACACTGTGC TCTAAACGCC
    ACCACGGCAG TCAACAGAGT GAAAGTGCGC AGAACAGAGG AAGAGTCGCC AAAGCCGGAG
    GAATCAGAAT CTATTATAGG CCGCGTTCTA AATCCCCAGG GGAAGGCAAA TAAGAGAATC
    TCATCAAAAG GAAAGAAAAC CCGTAGCGCA ACCCCCTCTC ACACCAAGGA TCTGAGTGCT
    TGCAGGGAAT CCCAGCACGA ACTGACAGCC AGCAACCAGT CCAGCCTGGG CCTCCTGAAC
    TCTATGATCC AGGAAGTAGA GCAGGATCTG GCCGAGTATG AGAGGCAGAC AGGACGGGAA
    GTGATATCCG CACCCCCAGC TCATGGTCTG ACAGGATTCA CTCTCTCTCT TGTCAGCTCG
    TTGAAACGAG TGGTGTCGTA CCTGAAGGAG AGTGACCTGC TGCTGCAGCG AGAGGTGAAG
    GAGAGGCAGC ACCTGCAGGG GGAGCTAGTG GAACAGAGGC TGATGCTCGA TGCTCTGACT
    GCAGAAATTC TCACCCTGAA AGAAGGAAAC AACATCCATG AGAATCACTT ACAGACCAAA
    CAGCGCCCAG AAGTAGGAGG GGACAAGATG GCTGCCATCC CCCAAGAAAT GGAAGAGCTT
    CCTGTAGTCA GTGAAGAACC TTGCAGAACC TCAGCTGAGT GCAACCTTTC CAAGGTCAAT
    CAATTCCTGG ATTCGCAGGA TGGGCCAGAT ACAGATGATA AACACGGCAG ACTTGGGGTG
    TCCAAGGAAG AATGTGGCCC CTGTTTGTAC CCCCAGGGCA GAGCTGCGGA GGAGCCCAGA
    TTAGCCAGCT CTGTACCCTC ACGTATGTTC CAGCAAGCTG TCTTGTTATC CCCTCCCAGG
    CAGAAAACTG TCAGTGAACT CAGCAGCCAA TCAGCAGTTC CCAAGAGTTA CAAACATAGT
    AACCGCCCAG CCCAGGGGGG CCAACAAGTC AGCCATCATC CTACTGCATC TACAGAAAAG
    GAACCCCCCA GCCAAGGACT AGAAATGGGT CAAAACCAAA TAAATCGCCT TCAAAACGAG
    GACCTTGTTT CCCATATGCA GCAGCTGGCG CTTCAGAATG CAGCACTTAA GGCTCAGCTT
    GGGCAAATAC ACTTTCCTCC AGCAGGAAAT GCCCCAGAAG GGGGCAAATC TGTGCCAGCC
    GAAGAGAACC GTAATCCAGA ACCTGCTACG GATCCACCGC AGAACAAAGC AGCTCAGGTC
    CCAGGGAGCC TGGAGATGCG CATCGCAGAG TTAAACAGGC AGAGTGCAGA AGCGCGGAAC
    AAGCTCCTAA AACTTATTGA GCAGCAGAAG CAGAGCATTG TGGTTTCTCC CACATTATCT
    CCCATAACCC CCCAGGACAG GCGCACAGAT TCCTCCCTGG ATACGACCCC CCCTTCCTCC
    TGCAGGACCT CGGGCACAAG GTTCTCCAGT GCATCTAACA AGAGCACCTC TTCCATCAAC
    TCGTCTTTTG GGAGTGTGAG ATCGGCCTCT GCTGGGCGGA GGTCCCAGGT ACGGTATAAA
    CCTGTATTGC TGAGCTGTAG TACGGTGTCT GCACTGGGCC AAAATCAAGC AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32205
    Clone ID Related Accession (Same CDS sequence) XM_012956518.2 , XM_031895713.1 , XM_031895712.1 , XM_012956518.3
    Accession Version XM_031895712.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spindle and centriole-associated protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTTATT TAAGAGCCGG CAGGACTTCA ACGAATGTTT CACTCACAAA GAAGCCATCG 
    AAGGGCAAAA AGAAACCGCA GGCCAGAAAG GAATGGGATA GTACAGTCAG TGATCTCACG
    GTGCTCCGTG CCACAAATGA AGAGCTGGAG CACCGGCGGG AGATACACAG ATCCAAGAAC
    CAGTGGCTGG CTCGTTGGGA ACTCCAGAAC AAAGCGACAA ATAAAAAGTC GGAGAAAGAA
    AATGGAGGAT ATTCAGCTCT TTTGGAAAAT TCACGCCTAG CTCTAATGAA GGAGATCCTT
    TCTGATCAGT ATTTGATGAA TGATGTATTG GAGAGGTCAG ACCGAGCCCT TGCTGTAGTG
    AAGGACCTGT TTGGAGATGC TCCTCGCAGA CACACAGGAT TTCCCAGTGT GACTATGGCT
    CCTTCCTGTG ATCTGGACAC ATCCCGAGCA CCCATAGTGC AGAAGAAGGA TCCCCCAACC
    CAGCTTTCTA TTCTGAGCGA GTCAATCATG GATTCCCAGG CTATCAATGA AGTGGACAAA
    TCACTGAGTC CGGCTGAATA CAGCGACAGT GAAGCTGAGG TTACTATTAG CACGCAACCC
    AACAGAAAGG CAGAGAGGGG ACAACAGTTG CTGAGTGGGG AGGGACCAAA GAATGAGGCC
    TTTATAACCC CTTGCAAATC AGGAGGTGAC CCATCGCAAA CACACTGTGC TCTAAACGCC
    ACCACGGCAG TCAACAGAGT GAAAGTGCGC AGAACAGAGG AAGAGTCGCC AAAGCCGGAG
    GAATCAGAAT CTATTATAGG CCGCGTTCTA AATCCCCAGG GGAAGGCAAA TAAGAGAATC
    TCATCAAAAG GAAAGAAAAC CCGTAGCGCA ACCCCCTCTC ACACCAAGGA TCTGAGTGCT
    TGCAGGGAAT CCCAGCACGA ACTGACAGCC AGCAACCAGT CCAGCCTGGG CCTCCTGAAC
    TCTATGATCC AGGAAGTAGA GCAGGATCTG GCCGAGTATG AGAGGCAGAC AGGACGGGAA
    GTGATATCCG CACCCCCAGC TCATGGTCTG ACAGGATTCA CTCTCTCTCT TGTCAGCTCG
    TTGAAACGAG TGGTGTCGTA CCTGAAGGAG AGTGACCTGC TGCTGCAGCG AGAGGTGAAG
    GAGAGGCAGC ACCTGCAGGG GGAGCTAGTG GAACAGAGGC TGATGCTCGA TGCTCTGACT
    GCAGAAATTC TCACCCTGAA AGAAGGAAAC AACATCCATG AGAATCACTT ACAGACCAAA
    CAGCGCCCAG AAGTAGGAGG GGACAAGATG GCTGCCATCC CCCAAGAAAT GGAAGAGCTT
    CCTGTAGTCA GTGAAGAACC TTGCAGAACC TCAGCTGAGT GCAACCTTTC CAAGGTCAAT
    CAATTCCTGG ATTCGCAGGA TGGGCCAGAT ACAGATGATA AACACGGCAG ACTTGGGGTG
    TCCAAGGAAG AATGTGGCCC CTGTTTGTAC CCCCAGGGCA GAGCTGCGGA GGAGCCCAGA
    TTAGCCAGCT CTGTACCCTC ACGTATGTTC CAGCAAGCTG TCTTGTTATC CCCTCCCAGG
    CAGAAAACTG TCAGTGAACT CAGCAGCCAA TCAGCAGTTC CCAAGAGTTA CAAACATAGT
    AACCGCCCAG CCCAGGGGGG CCAACAAGTC AGCCATCATC CTACTGCATC TACAGAAAAG
    GAACCCCCCA GCCAAGGACT AGAAATGGGT CAAAACCAAA TAAATCGCCT TCAAAACGAG
    GACCTTGTTT CCCATATGCA GCAGCTGGCG CTTCAGAATG CAGCACTTAA GGCTCAGCTT
    GGGCAAATAC ACTTTCCTCC AGCAGGAAAT GCCCCAGAAG GGGGCAAATC TGTGCCAGCC
    GAAGAGAACC GTAATCCAGA ACCTGCTACG GATCCACCGC AGAACAAAGC AGCTCAGGTC
    CCAGGGAGCC TGGAGATGCG CATCGCAGAG TTAAACAGGC AGAGTGCAGA AGCGCGGAAC
    AAGCTCCTAA AACTTATTGA GCAGCAGAAG CAGAGCATTG TGGTTTCTCC CACATTATCT
    CCCATAACCC CCCAGGACAG GCGCACAGAT TCCTCCCTGG ATACGACCCC CCCTTCCTCC
    TGCAGGACCT CGGGCACAAG GTTCTCCAGT GCATCTAACA AGAGCACCTC TTCCATCAAC
    TCGTCTTTTG GGAGTGTGAG ATCGGCCTCT GCTGGGCGGA GGTCCCAGGT ACGGTATAAA
    CCTGTATTGC TGAGCTGTAG TACGGTGTCT GCACTGGGCC AAAATCAAGC AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751572.1
    CDS25..2301
    Translation

    Target ORF information:

    RefSeq Version XM_031895712.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895712.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTTATT TAAGAGCCGG CAGGACTTCA ACGAATGTTT CACTCACAAA GAAGCCATCG 
    AAGGGCAAAA AGAAACCGCA GGCCAGAAAG GAATGGGATA GTACAGTCAG TGATCTCACG
    GTGCTCCGTG CCACAAATGA AGAGCTGGAG CACCGGCGGG AGATACACAG ATCCAAGAAC
    CAGTGGCTGG CTCGTTGGGA ACTCCAGAAC AAAGCGACAA ATAAAAAGTC GGAGAAAGAA
    AATGGAGGAT ATTCAGCTCT TTTGGAAAAT TCACGCCTAG CTCTAATGAA GGAGATCCTT
    TCTGATCAGT ATTTGATGAA TGATGTATTG GAGAGGTCAG ACCGAGCCCT TGCTGTAGTG
    AAGGACCTGT TTGGAGATGC TCCTCGCAGA CACACAGGAT TTCCCAGTGT GACTATGGCT
    CCTTCCTGTG ATCTGGACAC ATCCCGAGCA CCCATAGTGC AGAAGAAGGA TCCCCCAACC
    CAGCTTTCTA TTCTGAGCGA GTCAATCATG GATTCCCAGG CTATCAATGA AGTGGACAAA
    TCACTGAGTC CGGCTGAATA CAGCGACAGT GAAGCTGAGG TTACTATTAG CACGCAACCC
    AACAGAAAGG CAGAGAGGGG ACAACAGTTG CTGAGTGGGG AGGGACCAAA GAATGAGGCC
    TTTATAACCC CTTGCAAATC AGGAGGTGAC CCATCGCAAA CACACTGTGC TCTAAACGCC
    ACCACGGCAG TCAACAGAGT GAAAGTGCGC AGAACAGAGG AAGAGTCGCC AAAGCCGGAG
    GAATCAGAAT CTATTATAGG CCGCGTTCTA AATCCCCAGG GGAAGGCAAA TAAGAGAATC
    TCATCAAAAG GAAAGAAAAC CCGTAGCGCA ACCCCCTCTC ACACCAAGGA TCTGAGTGCT
    TGCAGGGAAT CCCAGCACGA ACTGACAGCC AGCAACCAGT CCAGCCTGGG CCTCCTGAAC
    TCTATGATCC AGGAAGTAGA GCAGGATCTG GCCGAGTATG AGAGGCAGAC AGGACGGGAA
    GTGATATCCG CACCCCCAGC TCATGGTCTG ACAGGATTCA CTCTCTCTCT TGTCAGCTCG
    TTGAAACGAG TGGTGTCGTA CCTGAAGGAG AGTGACCTGC TGCTGCAGCG AGAGGTGAAG
    GAGAGGCAGC ACCTGCAGGG GGAGCTAGTG GAACAGAGGC TGATGCTCGA TGCTCTGACT
    GCAGAAATTC TCACCCTGAA AGAAGGAAAC AACATCCATG AGAATCACTT ACAGACCAAA
    CAGCGCCCAG AAGTAGGAGG GGACAAGATG GCTGCCATCC CCCAAGAAAT GGAAGAGCTT
    CCTGTAGTCA GTGAAGAACC TTGCAGAACC TCAGCTGAGT GCAACCTTTC CAAGGTCAAT
    CAATTCCTGG ATTCGCAGGA TGGGCCAGAT ACAGATGATA AACACGGCAG ACTTGGGGTG
    TCCAAGGAAG AATGTGGCCC CTGTTTGTAC CCCCAGGGCA GAGCTGCGGA GGAGCCCAGA
    TTAGCCAGCT CTGTACCCTC ACGTATGTTC CAGCAAGCTG TCTTGTTATC CCCTCCCAGG
    CAGAAAACTG TCAGTGAACT CAGCAGCCAA TCAGCAGTTC CCAAGAGTTA CAAACATAGT
    AACCGCCCAG CCCAGGGGGG CCAACAAGTC AGCCATCATC CTACTGCATC TACAGAAAAG
    GAACCCCCCA GCCAAGGACT AGAAATGGGT CAAAACCAAA TAAATCGCCT TCAAAACGAG
    GACCTTGTTT CCCATATGCA GCAGCTGGCG CTTCAGAATG CAGCACTTAA GGCTCAGCTT
    GGGCAAATAC ACTTTCCTCC AGCAGGAAAT GCCCCAGAAG GGGGCAAATC TGTGCCAGCC
    GAAGAGAACC GTAATCCAGA ACCTGCTACG GATCCACCGC AGAACAAAGC AGCTCAGGTC
    CCAGGGAGCC TGGAGATGCG CATCGCAGAG TTAAACAGGC AGAGTGCAGA AGCGCGGAAC
    AAGCTCCTAA AACTTATTGA GCAGCAGAAG CAGAGCATTG TGGTTTCTCC CACATTATCT
    CCCATAACCC CCCAGGACAG GCGCACAGAT TCCTCCCTGG ATACGACCCC CCCTTCCTCC
    TGCAGGACCT CGGGCACAAG GTTCTCCAGT GCATCTAACA AGAGCACCTC TTCCATCAAC
    TCGTCTTTTG GGAGTGTGAG ATCGGCCTCT GCTGGGCGGA GGTCCCAGGT ACGGTATAAA
    CCTGTATTGC TGAGCTGTAG TACGGTGTCT GCACTGGGCC AAAATCAAGC AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32205
    Clone ID Related Accession (Same CDS sequence) XM_012956518.2 , XM_031895713.1 , XM_031895712.1 , XM_012956518.3
    Accession Version XM_012956518.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spindle and centriole-associated protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956518.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTTATT TAAGAGCCGG CAGGACTTCA ACGAATGTTT CACTCACAAA GAAGCCATCG 
    AAGGGCAAAA AGAAACCGCA GGCCAGAAAG GAATGGGATA GTACAGTCAG TGATCTCACG
    GTGCTCCGTG CCACAAATGA AGAGCTGGAG CACCGGCGGG AGATACACAG ATCCAAGAAC
    CAGTGGCTGG CTCGTTGGGA ACTCCAGAAC AAAGCGACAA ATAAAAAGTC GGAGAAAGAA
    AATGGAGGAT ATTCAGCTCT TTTGGAAAAT TCACGCCTAG CTCTAATGAA GGAGATCCTT
    TCTGATCAGT ATTTGATGAA TGATGTATTG GAGAGGTCAG ACCGAGCCCT TGCTGTAGTG
    AAGGACCTGT TTGGAGATGC TCCTCGCAGA CACACAGGAT TTCCCAGTGT GACTATGGCT
    CCTTCCTGTG ATCTGGACAC ATCCCGAGCA CCCATAGTGC AGAAGAAGGA TCCCCCAACC
    CAGCTTTCTA TTCTGAGCGA GTCAATCATG GATTCCCAGG CTATCAATGA AGTGGACAAA
    TCACTGAGTC CGGCTGAATA CAGCGACAGT GAAGCTGAGG TTACTATTAG CACGCAACCC
    AACAGAAAGG CAGAGAGGGG ACAACAGTTG CTGAGTGGGG AGGGACCAAA GAATGAGGCC
    TTTATAACCC CTTGCAAATC AGGAGGTGAC CCATCGCAAA CACACTGTGC TCTAAACGCC
    ACCACGGCAG TCAACAGAGT GAAAGTGCGC AGAACAGAGG AAGAGTCGCC AAAGCCGGAG
    GAATCAGAAT CTATTATAGG CCGCGTTCTA AATCCCCAGG GGAAGGCAAA TAAGAGAATC
    TCATCAAAAG GAAAGAAAAC CCGTAGCGCA ACCCCCTCTC ACACCAAGGA TCTGAGTGCT
    TGCAGGGAAT CCCAGCACGA ACTGACAGCC AGCAACCAGT CCAGCCTGGG CCTCCTGAAC
    TCTATGATCC AGGAAGTAGA GCAGGATCTG GCCGAGTATG AGAGGCAGAC AGGACGGGAA
    GTGATATCCG CACCCCCAGC TCATGGTCTG ACAGGATTCA CTCTCTCTCT TGTCAGCTCG
    TTGAAACGAG TGGTGTCGTA CCTGAAGGAG AGTGACCTGC TGCTGCAGCG AGAGGTGAAG
    GAGAGGCAGC ACCTGCAGGG GGAGCTAGTG GAACAGAGGC TGATGCTCGA TGCTCTGACT
    GCAGAAATTC TCACCCTGAA AGAAGGAAAC AACATCCATG AGAATCACTT ACAGACCAAA
    CAGCGCCCAG AAGTAGGAGG GGACAAGATG GCTGCCATCC CCCAAGAAAT GGAAGAGCTT
    CCTGTAGTCA GTGAAGAACC TTGCAGAACC TCAGCTGAGT GCAACCTTTC CAAGGTCAAT
    CAATTCCTGG ATTCGCAGGA TGGGCCAGAT ACAGATGATA AACACGGCAG ACTTGGGGTG
    TCCAAGGAAG AATGTGGCCC CTGTTTGTAC CCCCAGGGCA GAGCTGCGGA GGAGCCCAGA
    TTAGCCAGCT CTGTACCCTC ACGTATGTTC CAGCAAGCTG TCTTGTTATC CCCTCCCAGG
    CAGAAAACTG TCAGTGAACT CAGCAGCCAA TCAGCAGTTC CCAAGAGTTA CAAACATAGT
    AACCGCCCAG CCCAGGGGGG CCAACAAGTC AGCCATCATC CTACTGCATC TACAGAAAAG
    GAACCCCCCA GCCAAGGACT AGAAATGGGT CAAAACCAAA TAAATCGCCT TCAAAACGAG
    GACCTTGTTT CCCATATGCA GCAGCTGGCG CTTCAGAATG CAGCACTTAA GGCTCAGCTT
    GGGCAAATAC ACTTTCCTCC AGCAGGAAAT GCCCCAGAAG GGGGCAAATC TGTGCCAGCC
    GAAGAGAACC GTAATCCAGA ACCTGCTACG GATCCACCGC AGAACAAAGC AGCTCAGGTC
    CCAGGGAGCC TGGAGATGCG CATCGCAGAG TTAAACAGGC AGAGTGCAGA AGCGCGGAAC
    AAGCTCCTAA AACTTATTGA GCAGCAGAAG CAGAGCATTG TGGTTTCTCC CACATTATCT
    CCCATAACCC CCCAGGACAG GCGCACAGAT TCCTCCCTGG ATACGACCCC CCCTTCCTCC
    TGCAGGACCT CGGGCACAAG GTTCTCCAGT GCATCTAACA AGAGCACCTC TTCCATCAAC
    TCGTCTTTTG GGAGTGTGAG ATCGGCCTCT GCTGGGCGGA GGTCCCAGGT ACGGTATAAA
    CCTGTATTGC TGAGCTGTAG TACGGTGTCT GCACTGGGCC AAAATCAAGC AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811972.1
    CDS369..2645
    Translation

    Target ORF information:

    RefSeq Version XM_012956518.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956518.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTTATT TAAGAGCCGG CAGGACTTCA ACGAATGTTT CACTCACAAA GAAGCCATCG 
    AAGGGCAAAA AGAAACCGCA GGCCAGAAAG GAATGGGATA GTACAGTCAG TGATCTCACG
    GTGCTCCGTG CCACAAATGA AGAGCTGGAG CACCGGCGGG AGATACACAG ATCCAAGAAC
    CAGTGGCTGG CTCGTTGGGA ACTCCAGAAC AAAGCGACAA ATAAAAAGTC GGAGAAAGAA
    AATGGAGGAT ATTCAGCTCT TTTGGAAAAT TCACGCCTAG CTCTAATGAA GGAGATCCTT
    TCTGATCAGT ATTTGATGAA TGATGTATTG GAGAGGTCAG ACCGAGCCCT TGCTGTAGTG
    AAGGACCTGT TTGGAGATGC TCCTCGCAGA CACACAGGAT TTCCCAGTGT GACTATGGCT
    CCTTCCTGTG ATCTGGACAC ATCCCGAGCA CCCATAGTGC AGAAGAAGGA TCCCCCAACC
    CAGCTTTCTA TTCTGAGCGA GTCAATCATG GATTCCCAGG CTATCAATGA AGTGGACAAA
    TCACTGAGTC CGGCTGAATA CAGCGACAGT GAAGCTGAGG TTACTATTAG CACGCAACCC
    AACAGAAAGG CAGAGAGGGG ACAACAGTTG CTGAGTGGGG AGGGACCAAA GAATGAGGCC
    TTTATAACCC CTTGCAAATC AGGAGGTGAC CCATCGCAAA CACACTGTGC TCTAAACGCC
    ACCACGGCAG TCAACAGAGT GAAAGTGCGC AGAACAGAGG AAGAGTCGCC AAAGCCGGAG
    GAATCAGAAT CTATTATAGG CCGCGTTCTA AATCCCCAGG GGAAGGCAAA TAAGAGAATC
    TCATCAAAAG GAAAGAAAAC CCGTAGCGCA ACCCCCTCTC ACACCAAGGA TCTGAGTGCT
    TGCAGGGAAT CCCAGCACGA ACTGACAGCC AGCAACCAGT CCAGCCTGGG CCTCCTGAAC
    TCTATGATCC AGGAAGTAGA GCAGGATCTG GCCGAGTATG AGAGGCAGAC AGGACGGGAA
    GTGATATCCG CACCCCCAGC TCATGGTCTG ACAGGATTCA CTCTCTCTCT TGTCAGCTCG
    TTGAAACGAG TGGTGTCGTA CCTGAAGGAG AGTGACCTGC TGCTGCAGCG AGAGGTGAAG
    GAGAGGCAGC ACCTGCAGGG GGAGCTAGTG GAACAGAGGC TGATGCTCGA TGCTCTGACT
    GCAGAAATTC TCACCCTGAA AGAAGGAAAC AACATCCATG AGAATCACTT ACAGACCAAA
    CAGCGCCCAG AAGTAGGAGG GGACAAGATG GCTGCCATCC CCCAAGAAAT GGAAGAGCTT
    CCTGTAGTCA GTGAAGAACC TTGCAGAACC TCAGCTGAGT GCAACCTTTC CAAGGTCAAT
    CAATTCCTGG ATTCGCAGGA TGGGCCAGAT ACAGATGATA AACACGGCAG ACTTGGGGTG
    TCCAAGGAAG AATGTGGCCC CTGTTTGTAC CCCCAGGGCA GAGCTGCGGA GGAGCCCAGA
    TTAGCCAGCT CTGTACCCTC ACGTATGTTC CAGCAAGCTG TCTTGTTATC CCCTCCCAGG
    CAGAAAACTG TCAGTGAACT CAGCAGCCAA TCAGCAGTTC CCAAGAGTTA CAAACATAGT
    AACCGCCCAG CCCAGGGGGG CCAACAAGTC AGCCATCATC CTACTGCATC TACAGAAAAG
    GAACCCCCCA GCCAAGGACT AGAAATGGGT CAAAACCAAA TAAATCGCCT TCAAAACGAG
    GACCTTGTTT CCCATATGCA GCAGCTGGCG CTTCAGAATG CAGCACTTAA GGCTCAGCTT
    GGGCAAATAC ACTTTCCTCC AGCAGGAAAT GCCCCAGAAG GGGGCAAATC TGTGCCAGCC
    GAAGAGAACC GTAATCCAGA ACCTGCTACG GATCCACCGC AGAACAAAGC AGCTCAGGTC
    CCAGGGAGCC TGGAGATGCG CATCGCAGAG TTAAACAGGC AGAGTGCAGA AGCGCGGAAC
    AAGCTCCTAA AACTTATTGA GCAGCAGAAG CAGAGCATTG TGGTTTCTCC CACATTATCT
    CCCATAACCC CCCAGGACAG GCGCACAGAT TCCTCCCTGG ATACGACCCC CCCTTCCTCC
    TGCAGGACCT CGGGCACAAG GTTCTCCAGT GCATCTAACA AGAGCACCTC TTCCATCAAC
    TCGTCTTTTG GGAGTGTGAG ATCGGCCTCT GCTGGGCGGA GGTCCCAGGT ACGGTATAAA
    CCTGTATTGC TGAGCTGTAG TACGGTGTCT GCACTGGGCC AAAATCAAGC AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42460
    Clone ID Related Accession (Same CDS sequence) XM_018091099.1
    Accession Version XM_018091099.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spindle and centriole-associated protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGATCACT CTTCAGGTTC ACTTTCAATG TCTTATTTAA GAGCCGGCAG GACTTCAACG 
    AATGTTTCAC TCACAAAGAA GCCATCGAAG GGCAAAAAGA AACCGCAGGC CAGAAAGGAA
    TGGGATAGTA CAGTCAGTGA TCTCACGGTG CTCCGTGCCA CAAATGAAGA GCTGGAGCAC
    CGGCGGGAGA TACACAGATC CAAGAACCAG TGGCTGGCTC GTTGGGAACT CCAGAACAAA
    GCGACAAATA AAAAGTCGGA GAAAGAAAAT GGAGGATATT CAGCTCTTTT GGAAAATTCA
    CGCCTAGCTC TAATGAAGGA GATCCTTTCT GATCAGTATT TGATGAATGA TGTATTGGAG
    AGGTCAGACC GAGCCCTTGC TGTAGTGAAG GACCTGTTTG GAGATGCTCC TCGCAGACAC
    ACAGGATTTC CCAGTGTGAC TATGGCTCCT TCCTGTGATC TGGACACATC CCGAGCACCC
    ATAGTGCAGA AGAAGGATCC CCCAACCCAG CTTTCTATTC TGAGCGAGTC AATCATGGAT
    TCCCAGGCTA TCAATGAAGT GGACAAATCA CTGAGTCCGG CTGAATACAG CGACAGTGAA
    GCTGAGGTTA CTATTAGCAC GCAACCCAAC AGAAAGGCAG AGAGGGGACA ACAGTTGCTG
    AGTGGGGAGG GACCAAAGAA TGAGGCCTTT ATAACCCCTT GCAAATCAGG AGGTGACCCA
    TCGCAAACAC ACTGTGCTCT AAACGCCACC ACGGCAGTCA ACAGAGTGAA AGTGCGCAGA
    ACAGAGGAAG AGTCGCCAAA GCCGGAGGAA TCAGAATCTA TTATAGGCCG CGTTCTAAAT
    CCCCAGGGGA AGGCAAATAA GAGAATCTCA TCAAAAGGAA AGAAAACCCG TAGCGCAACC
    CCCTCTCACA CCAAGGATCT GAGTGCTTGC AGGGAATCCC AGCACGAACT GACAGCCAGC
    AACCAGTCCA GCCTGGGCCT CCTGAACTCT ATGATCCAGG AAGTAGAGCA GGATCTGGCC
    GAGTATGAGA GGCAGACAGG ACGGGAAGTG ATATCCGCAC CCCCAGCTCA TGGTCTGACA
    GGATTCACTC TCTCTCTTGT CAGCTCGTTG AAACGAGTGG TGTCGTACCT GAAGGAGAGT
    GACCTGCTGC TGCAGCGAGA GGTGAAGGAG AGGCAGCACC TGCAGGGGGA GCTAGTGGAA
    CAGAGGCTGA TGCTCGATGC TCTGACTGCA GAAATTCTCA CCCTGAAAGA AGGAAACAAC
    ATCCATGAGA ATCACTTACA GACCAAACAG CGCCCAGAAG TAGGAGGGGA CAAGATGGCT
    GCCATCCCCC AAGAAATGGA AGAGCTTCCT GTAGTCAGTG AAGAACCTTG CAGAACCTCA
    GCTGAGTGCA ACCTTTCCAA GGTCAATCAA TTCCTGGATT CGCAGGATGG GCCAGATACA
    GATGATAAAC ACGGCAGACT TGGGGTGTCC AAGGAAGAAT GTGGCCCCTG TTTGTACCCC
    CAGGGCAGAG CTGCGGAGGA GCCCAGATTA GCCAGCTCTG TACCCTCACG TATGTTCCAG
    CAAGCTGTCT TGTTATCCCC TCCCAGGCAG AAAACTGTCA GTGAACTCAG CAGCCAATCA
    GCAGTTCCCA AGAGTTACAA ACATAGTAAC CGCCCAGCCC AGGGGGGCCA ACAAGTCAGC
    CATCATCCTA CTGCATCTAC AGAAAAGGAA CCCCCCAGCC AAGGACTAGA AATGGGTCAA
    AACCAAATAA ATCGCCTTCA AAACGAGGAC CTTGTTTCCC ATATGCAGCA GCTGGCGCTT
    CAGAATGCAG CACTTAAGGC TCAGCTTGGG CAAATACACT TTCCTCCAGC AGGAAATGCC
    CCAGAAGGGG GCAAATCTGT GCCAGCCGAA GAGAACCGTA ATCCAGAACC TGCTACGGAT
    CCACCGATTC CTCCCTGGAT ACGACCCCCC CTTCCTCCTG CAGGACCTCG GGCACAAGGT
    TCTCCAGTGC ATCTAACAAG AGCACCTCTT CCATCAACTC GTCTTTTGGG AGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946588.1
    CDS381..2417
    Translation

    Target ORF information:

    RefSeq Version XM_018091099.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spindle and centriole associated protein 1 (spice1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091099.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGATCACT CTTCAGGTTC ACTTTCAATG TCTTATTTAA GAGCCGGCAG GACTTCAACG 
    AATGTTTCAC TCACAAAGAA GCCATCGAAG GGCAAAAAGA AACCGCAGGC CAGAAAGGAA
    TGGGATAGTA CAGTCAGTGA TCTCACGGTG CTCCGTGCCA CAAATGAAGA GCTGGAGCAC
    CGGCGGGAGA TACACAGATC CAAGAACCAG TGGCTGGCTC GTTGGGAACT CCAGAACAAA
    GCGACAAATA AAAAGTCGGA GAAAGAAAAT GGAGGATATT CAGCTCTTTT GGAAAATTCA
    CGCCTAGCTC TAATGAAGGA GATCCTTTCT GATCAGTATT TGATGAATGA TGTATTGGAG
    AGGTCAGACC GAGCCCTTGC TGTAGTGAAG GACCTGTTTG GAGATGCTCC TCGCAGACAC
    ACAGGATTTC CCAGTGTGAC TATGGCTCCT TCCTGTGATC TGGACACATC CCGAGCACCC
    ATAGTGCAGA AGAAGGATCC CCCAACCCAG CTTTCTATTC TGAGCGAGTC AATCATGGAT
    TCCCAGGCTA TCAATGAAGT GGACAAATCA CTGAGTCCGG CTGAATACAG CGACAGTGAA
    GCTGAGGTTA CTATTAGCAC GCAACCCAAC AGAAAGGCAG AGAGGGGACA ACAGTTGCTG
    AGTGGGGAGG GACCAAAGAA TGAGGCCTTT ATAACCCCTT GCAAATCAGG AGGTGACCCA
    TCGCAAACAC ACTGTGCTCT AAACGCCACC ACGGCAGTCA ACAGAGTGAA AGTGCGCAGA
    ACAGAGGAAG AGTCGCCAAA GCCGGAGGAA TCAGAATCTA TTATAGGCCG CGTTCTAAAT
    CCCCAGGGGA AGGCAAATAA GAGAATCTCA TCAAAAGGAA AGAAAACCCG TAGCGCAACC
    CCCTCTCACA CCAAGGATCT GAGTGCTTGC AGGGAATCCC AGCACGAACT GACAGCCAGC
    AACCAGTCCA GCCTGGGCCT CCTGAACTCT ATGATCCAGG AAGTAGAGCA GGATCTGGCC
    GAGTATGAGA GGCAGACAGG ACGGGAAGTG ATATCCGCAC CCCCAGCTCA TGGTCTGACA
    GGATTCACTC TCTCTCTTGT CAGCTCGTTG AAACGAGTGG TGTCGTACCT GAAGGAGAGT
    GACCTGCTGC TGCAGCGAGA GGTGAAGGAG AGGCAGCACC TGCAGGGGGA GCTAGTGGAA
    CAGAGGCTGA TGCTCGATGC TCTGACTGCA GAAATTCTCA CCCTGAAAGA AGGAAACAAC
    ATCCATGAGA ATCACTTACA GACCAAACAG CGCCCAGAAG TAGGAGGGGA CAAGATGGCT
    GCCATCCCCC AAGAAATGGA AGAGCTTCCT GTAGTCAGTG AAGAACCTTG CAGAACCTCA
    GCTGAGTGCA ACCTTTCCAA GGTCAATCAA TTCCTGGATT CGCAGGATGG GCCAGATACA
    GATGATAAAC ACGGCAGACT TGGGGTGTCC AAGGAAGAAT GTGGCCCCTG TTTGTACCCC
    CAGGGCAGAG CTGCGGAGGA GCCCAGATTA GCCAGCTCTG TACCCTCACG TATGTTCCAG
    CAAGCTGTCT TGTTATCCCC TCCCAGGCAG AAAACTGTCA GTGAACTCAG CAGCCAATCA
    GCAGTTCCCA AGAGTTACAA ACATAGTAAC CGCCCAGCCC AGGGGGGCCA ACAAGTCAGC
    CATCATCCTA CTGCATCTAC AGAAAAGGAA CCCCCCAGCC AAGGACTAGA AATGGGTCAA
    AACCAAATAA ATCGCCTTCA AAACGAGGAC CTTGTTTCCC ATATGCAGCA GCTGGCGCTT
    CAGAATGCAG CACTTAAGGC TCAGCTTGGG CAAATACACT TTCCTCCAGC AGGAAATGCC
    CCAGAAGGGG GCAAATCTGT GCCAGCCGAA GAGAACCGTA ATCCAGAACC TGCTACGGAT
    CCACCGATTC CTCCCTGGAT ACGACCCCCC CTTCCTCCTG CAGGACCTCG GGCACAAGGT
    TCTCCAGTGC ATCTAACAAG AGCACCTCTT CCATCAACTC GTCTTTTGGG AGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.