rapgefl1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	rapgefl1
Entrez Gene ID	100145761
Full Name	Rap guanine nucleotide exchange factor like 1
General protein information	Preferred Names Rap guanine nucleotide exchange factor like 1 Names rap guanine nucleotide exchange factor-like 1 Rap guanine nucleotide exchange factor (GEF)-like 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018089428.2	XP_017944917.1	rap guanine nucleotide exchange factor-like 1 isoform X2
XM_018089428.1	XP_017944917.1	rap guanine nucleotide exchange factor-like 1 isoform X2
NM_001127132.1	NP_001120604.1	rap guanine nucleotide exchange factor-like 1
XM_031893985.1	XP_031749845.1	rap guanine nucleotide exchange factor-like 1 isoform X1
XM_012971530.3	XP_012826984.1	rap guanine nucleotide exchange factor-like 1 isoform X1
XM_031893986.1	XP_031749846.1	rap guanine nucleotide exchange factor-like 1 isoform X2
XM_012971530.2	XP_012826984.1	rap guanine nucleotide exchange factor-like 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RAPGEFL1	NP_057423.1
	RAPGEFL1	XP_001096654.1
Xenopus tropicalis (tropical clawed frog)	rapgefl1	NP_001120604.1
Canis lupus familiaris (dog)	RAPGEFL1	XP_548135.3
Pan troglodytes (chimpanzee)	RAPGEFL1	XP_001170594.3
Bos taurus (cattle)	RAPGEFL1	NP_001192336.1
Mus musculus (house mouse)	Rapgefl1	NP_001074394.1
Rattus norvegicus (Norway rat)	Rapgefl1	XP_006247549.1
Danio rerio (zebrafish)	LOC100149497	XP_001921020.3

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rapgefl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rapgefl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa21221	NM_001127132.1 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa32038	XM_012971530.2 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa32039	XM_018089428.1 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa49965	XM_031893985.1 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa49965	XM_012971530.3 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa49966	XM_018089428.2 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa49966	XM_031893986.1 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rapgefl1 ( NM_001127132.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001120604.1 Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), mRNA.

Price & Availability↑

CloneID	OXa21221
Clone ID Related Accession (Same CDS sequence)	NM_001127132.1
Accession Version	NM_001127132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor-like 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC161641.1. ##Evidence-Data-START## Transcript exon combination :: BC161641.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGAAACCTC TGGAAAAATT CTTGAAAAAG CAAAGCTCTC ATCTTTCTGG ACCCCGACAC 
CTACACAGAA GGCAGAGCGT CTCCAAAATC CTGCCTTCTT TCCTGAGGGA TACTCCGGGG 
GACTCCTCTG GGGGAGAAAG GTGTGAGGAA GATCCATCAG GTGGGCCGCC GCCATCCCAG 
GACTGCCTGG AACTACCAGA TGGACTTCGG TCCCCTCTTT CTTTTTCTTC AGATGAACTA 
TCCCCCTCAG AACCCCTCAC ACCTCCGCCA GGATCTGGGG GCTGGACCCT TTCCCCTCCT 
GGCCCTCTGC TTGCCCCTGA TACCCCTGAG GCTCTACTCG GGAGGGTCCT GGAGCAGCTA 
CTGAGTGCGC CGAGGCTAAA TGAAGCAGCA GAATTGGCCC TGGACGACTT TATCCTCACG 
CATACGCTAT TTATGCCGAC AGAGGAGCTC CTCCTCCAAT TACAGCAGTA CTTCTGCGGC 
TGCAGCCGAT ATACTACTCC GGCGTGGGAA GGGAGCGACC CTCTTCAGAG GAAGCAGGCT 
GTACTCTCAT CTCTTTTACA CCTCTTGGAT ATTTACAAGG ACGCTCTCCA GGAGGAGGAG 
AGGACATTCC AGCTAATCAA GGATTTCTAT GTCTTGGTGA TGAGGGATGC CCCGAATTTC 
CCACAGCTGG AGGGGAGCGT CATCCGATTG CACAGGCTTG TGGAAAGCGC TGAGCTCAGG 
CTTACAGATG GCAGTGCCAC TCAATGCTCA AAGCAAGTCA AACCTCTCTT CCGCCATTTC 
CGAAGAATCG ACTCCTGCCT GCAGCCACGG GTGGCATTCA GGGGCTCTGA CGAGATTTTT 
TGCAGGGTCT ACATGCCTGA TCATTCGTAC GTTACAATTA GGAGCCGACT TTCTGCTTCT 
GTCTCTGATA TCCTGACCTC TGTTTCTGAG AAACTGCAAT ATTCAGAAGA ACAGGGGCAG 
AGAGAGGAAC CCCTGATCCT GGTGGCTGTG ACAAGTGCTG GAGACAAGGT TCTGCTGAAG 
CCAGATGATG GCTGTATCTT TACCACTCTG GGGATTAATA GTCACTTGTT CGCCTGCAAT 
CGGGAGGAGC TGCGCTCTTT GGTTCCCCTT CCAGAGGAAG TTCAACTGCC TCCCGAAGAC 
TCCCATATTC ACAGGATTGA GGCAGAAGAC TTGGCCAATC AACTGAGTGC TTTCCACTGG 
GAACTTTTCA GCTGTGTGCA TGAGCTGGAA TTTATAGACT ATGTGTTCCA CGTGGAGCGA 
GGACGCAGGG AGACGGCGAA TCTGGAACTT TTGTTGCAGA GGTGCAGTGA GGTGCAGCAC 
TGGGTCAGTA CTCAGACTCT GCTCTGCGAG GGGCCAGCAC GCCGCACACA ACTTCTCAAG 
AAATTCATCA AGACAGCTGC TATCTGTAAA CAAAACCAGG ACCTGCTGTC ATTCTTTGCA 
ATCGTGATGG GCTTGGATAA TGGAGCTGTG AGCCGCCTTC GGGTGACATG GGAAAAATTG 
CCAGGAAAAT GTAAGAACTT GTTCCGAAAG TTTGAGAATT TGACGGATCC GTGCCGGAAC 
CACAAATCCT ACCGTGAGAT GGTGTCTCGT ATGCGCCCTC CTGTAATTCC ATTTATACCC 
CTTATTCTGA AAGATCTCAC TTTTTTACAC GAGGGCAGCA AAACCTTCAT TGACGGCCTT 
GTGAATGTAG AAAAAATGCA CGCCATTGCT GAAAAAGTGA GGACTGTGCG AAAATACCGG 
AGTAACCAGC TACGGCTGGA TACCGATGTC TCCCCGACTC ATCTGCAAAA TAAAGCCTAC 
GTGCGCCAGT TCCAAGTTAT CGACAACCAG AACCTGCTGT TTGAGTTGTC CTGCAAACTG 
GAGCCCAATA CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120604.1
CDS	219..2096
Translation	MKPLEKFLKKQSSHLSGPRHLHRRQSVSKILPSFLRDTPGDSSGGERCEEDPSGGPPPSQDCLELPDGLRSPLSFSSDELSPSEPLTPPPGSGGWTLSPPGPLLAPDTPEALLGRVLEQLLSAPRLNEAAELALDDFILTHTLFMPTEELLLQLQQYFCGCSRYTTPAWEGSDPLQRKQAVLSSLLHLLDIYKDALQEEERTFQLIKDFYVLVMRDAPNFPQLEGSVIRLHRLVESAELRLTDGSATQCSKQVKPLFRHFRRIDSCLQPRVAFRGSDEIFCRVYMPDHSYVTIRSRLSASVSDILTSVSEKLQYSEEQGQREEPLILVAVTSAGDKVLLKPDDGCIFTTLGINSHLFACNREELRSLVPLPEEVQLPPEDSHIHRIEAEDLANQLSAFHWELFSCVHELEFIDYVFHVERGRRETANLELLLQRCSEVQHWVSTQTLLCEGPARRTQLLKKFIKTAAICKQNQDLLSFFAIVMGLDNGAVSRLRVTWEKLPGKCKNLFRKFENLTDPCRNHKSYREMVSRMRPPVIPFIPLILKDLTFLHEGSKTFIDGLVNVEKMHAIAEKVRTVRKYRSNQLRLDTDVSPTHLQNKAYVRQFQVIDNQNLLFELSCKLEPNTQ

Target ORF information:

RefSeq Version	NM_001127132.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127132.1

ORF Insert Sequence:

ATGAAACCTC TGGAAAAATT CTTGAAAAAG CAAAGCTCTC ATCTTTCTGG ACCCCGACAC 
CTACACAGAA GGCAGAGCGT CTCCAAAATC CTGCCTTCTT TCCTGAGGGA TACTCCGGGG 
GACTCCTCTG GGGGAGAAAG GTGTGAGGAA GATCCATCAG GTGGGCCGCC GCCATCCCAG 
GACTGCCTGG AACTACCAGA TGGACTTCGG TCCCCTCTTT CTTTTTCTTC AGATGAACTA 
TCCCCCTCAG AACCCCTCAC ACCTCCGCCA GGATCTGGGG GCTGGACCCT TTCCCCTCCT 
GGCCCTCTGC TTGCCCCTGA TACCCCTGAG GCTCTACTCG GGAGGGTCCT GGAGCAGCTA 
CTGAGTGCGC CGAGGCTAAA TGAAGCAGCA GAATTGGCCC TGGACGACTT TATCCTCACG 
CATACGCTAT TTATGCCGAC AGAGGAGCTC CTCCTCCAAT TACAGCAGTA CTTCTGCGGC 
TGCAGCCGAT ATACTACTCC GGCGTGGGAA GGGAGCGACC CTCTTCAGAG GAAGCAGGCT 
GTACTCTCAT CTCTTTTACA CCTCTTGGAT ATTTACAAGG ACGCTCTCCA GGAGGAGGAG 
AGGACATTCC AGCTAATCAA GGATTTCTAT GTCTTGGTGA TGAGGGATGC CCCGAATTTC 
CCACAGCTGG AGGGGAGCGT CATCCGATTG CACAGGCTTG TGGAAAGCGC TGAGCTCAGG 
CTTACAGATG GCAGTGCCAC TCAATGCTCA AAGCAAGTCA AACCTCTCTT CCGCCATTTC 
CGAAGAATCG ACTCCTGCCT GCAGCCACGG GTGGCATTCA GGGGCTCTGA CGAGATTTTT 
TGCAGGGTCT ACATGCCTGA TCATTCGTAC GTTACAATTA GGAGCCGACT TTCTGCTTCT 
GTCTCTGATA TCCTGACCTC TGTTTCTGAG AAACTGCAAT ATTCAGAAGA ACAGGGGCAG 
AGAGAGGAAC CCCTGATCCT GGTGGCTGTG ACAAGTGCTG GAGACAAGGT TCTGCTGAAG 
CCAGATGATG GCTGTATCTT TACCACTCTG GGGATTAATA GTCACTTGTT CGCCTGCAAT 
CGGGAGGAGC TGCGCTCTTT GGTTCCCCTT CCAGAGGAAG TTCAACTGCC TCCCGAAGAC 
TCCCATATTC ACAGGATTGA GGCAGAAGAC TTGGCCAATC AACTGAGTGC TTTCCACTGG 
GAACTTTTCA GCTGTGTGCA TGAGCTGGAA TTTATAGACT ATGTGTTCCA CGTGGAGCGA 
GGACGCAGGG AGACGGCGAA TCTGGAACTT TTGTTGCAGA GGTGCAGTGA GGTGCAGCAC 
TGGGTCAGTA CTCAGACTCT GCTCTGCGAG GGGCCAGCAC GCCGCACACA ACTTCTCAAG 
AAATTCATCA AGACAGCTGC TATCTGTAAA CAAAACCAGG ACCTGCTGTC ATTCTTTGCA 
ATCGTGATGG GCTTGGATAA TGGAGCTGTG AGCCGCCTTC GGGTGACATG GGAAAAATTG 
CCAGGAAAAT GTAAGAACTT GTTCCGAAAG TTTGAGAATT TGACGGATCC GTGCCGGAAC 
CACAAATCCT ACCGTGAGAT GGTGTCTCGT ATGCGCCCTC CTGTAATTCC ATTTATACCC 
CTTATTCTGA AAGATCTCAC TTTTTTACAC GAGGGCAGCA AAACCTTCAT TGACGGCCTT 
GTGAATGTAG AAAAAATGCA CGCCATTGCT GAAAAAGTGA GGACTGTGCG AAAATACCGG 
AGTAACCAGC TACGGCTGGA TACCGATGTC TCCCCGACTC ATCTGCAAAA TAAAGCCTAC 
GTGCGCCAGT TCCAAGTTAT CGACAACCAG AACCTGCTGT TTGAGTTGTC CTGCAAACTG 
GAGCCCAATA CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgefl1 ( XM_012971530.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826984.1 Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa32038
Clone ID Related Accession (Same CDS sequence)	XM_012971530.2
Accession Version	XM_012971530.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor-like 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683386.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971530.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCTC TGGAAAAATT CTTGAAAAAG CAAAGCTCTC ATCTTTCTGG ACCCCGACAC 
CTACACAGAA GGCAGAGCGT CTCCAAAATC CTGCCTTCTT TCCTGAGGGA TACTCCGGGG 
GACTCCTCTG GGGGAGAAAG GTGTGAGGAA GATCCATCAG GTGGGCCGCC GCCATCCCAG 
GACTGCCTGG AACTACCAGA TGGACTTCGG TCCCCTCTTT CTTTTTCTTC AGATGAACTA 
TCCCCCTCAG AACCCCTCAC ACCTCCGCCA GGATCTGGGG GCTGGACCCT TTCCCCTCCT 
GGCCCTCTGC TTGCCCCTGA TACCCCTGAG GCTCTACTCG GGAGGGTCCT GGAGCAGCTA 
CTGAGTGCGC CGAGGCTAAA TGAAGCAGCA GAATTGGCCC TGGACGACTT TATCCTCACG 
CATACGCTAT TTATGCCGAC AGAGGAGCTC CTCCTCCAAT TACAGCAGTA CTTCTGCGGC 
TGCAGCCGAT ATACTACTCC GGCGTGGGAA GGGAGCGACC CTCTTCAGAG GAAGCAGGCT 
GTACTCTCAT CTCTTTTACA CCTCTTGGAT ATTTACAAGG ACGCTCTCCA GGAGGAGGAG 
AGGACATTCC AGCTAATCAA GGATTTCTAT GTCTTGGTGA TGAGGGATGC CCCGAATTTC 
CCGCAGCTGG AGGGGAGCGT CATCCGATTG CACAGGCTTG TGGAAAGCGC TGAGCTCAGG 
CTTACAGATG GCAGTGCCAC TCAATGCTCA AAGCAAGTCA AACCTCTCTT CCGCCATTTC 
CGAAGAATCG ACTCCTGCCT GCAGCCACGG GTGGCATTCA GGGGCTCTGA CGAGATTTTT 
TGCAGGGTCT ACATGCCTGA TCATTCGTAC GTTACAATTA GGAGCCGACT TTCTGCTTCT 
GTCTCTGATA TCCTGACCTC TGTTTCTGAG AAACTGCAAT ATTCAGAAGA ACAGGGGCAG 
AGAGAGGAAC CCCTGATCCT GGTGGCTGTG ACAAGTGCTG GAGACAAGGT TCTGCTGAAG 
CCAGATGATG GCTGTATCTT TACCACTCTG GGGATTAATA GTCACTTGTT CGCCTGCAAT 
CGGGAGGAGC TGCGCTCTTT GGTTCCCCTT CCAGAGGAAG TTCAACTGCC TCCCGAAGAC 
TCCCATATTC ACAGGATTGA GGCAGAAGAC TTGGCCAATC AACTGAGTGC TTTCCACTGG 
GAACTTTTCA GCTGTGTGCA TGAGCTGGAA TTTATAGACT ATGTGTTCCA CGTGGAGCGA 
GGACGCAGGG AGACGGCGAA TCTGGAACTT TTGTTGCAGA GGTGCAGTGA GGTGCAGCAC 
TGGGTCAGTA CTCAGACTCT GCTCTGCGAG GGGCCAGCAC GCCGCACACA ACTTCTCAAG 
AAATTCATCA AGACAGCTGC TATCTGTAAA CAAAACCAGG ACCTGCTGTC ATTCTTTGCA 
ATCGTGATGG GCTTGGATAA TGGAGCTGTG AGCCGCCTTC GGGTGACATG GGAAGGTACT 
ATAGACAGGG CAAGAAATGC TCGCTGTCAG AAATTGCCAG GAAAATGTAA GAACTTGTTC 
CGAAAGTTTG AGAATTTGAC GGATCCGTGC CGGAACCACA AATCCTACCG TGAGATGGTG 
TCTCGTATGC GCCCTCCTGT AATTCCATTT ATACCCCTTA TTCTGAAAGA TCTCACTTTT 
TTACACGAGG GCAGCAAAAC CTTCATTGAC GGCCTTGTGA ATGTAGAAAA AATGCACGCC 
ATTGCTGAAA AAGTGAGGAC TGTGCGAAAA TACCGGAGTA ACCAGCTACG GCTGGATACC 
GATGTCTCCC CGACTCATCT GCAAAATAAA GCCTACGTGC GCCAGTTCCA AGTTATCGAC 
AACCAGAACC TGCTGTTTGA GTTGTCCTGC AAACTGGAGC CCAATACGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826984.1
CDS	748..2661
Translation	MKPLEKFLKKQSSHLSGPRHLHRRQSVSKILPSFLRDTPGDSSGGERCEEDPSGGPPPSQDCLELPDGLRSPLSFSSDELSPSEPLTPPPGSGGWTLSPPGPLLAPDTPEALLGRVLEQLLSAPRLNEAAELALDDFILTHTLFMPTEELLLQLQQYFCGCSRYTTPAWEGSDPLQRKQAVLSSLLHLLDIYKDALQEEERTFQLIKDFYVLVMRDAPNFPQLEGSVIRLHRLVESAELRLTDGSATQCSKQVKPLFRHFRRIDSCLQPRVAFRGSDEIFCRVYMPDHSYVTIRSRLSASVSDILTSVSEKLQYSEEQGQREEPLILVAVTSAGDKVLLKPDDGCIFTTLGINSHLFACNREELRSLVPLPEEVQLPPEDSHIHRIEAEDLANQLSAFHWELFSCVHELEFIDYVFHVERGRRETANLELLLQRCSEVQHWVSTQTLLCEGPARRTQLLKKFIKTAAICKQNQDLLSFFAIVMGLDNGAVSRLRVTWEGTIDRARNARCQKLPGKCKNLFRKFENLTDPCRNHKSYREMVSRMRPPVIPFIPLILKDLTFLHEGSKTFIDGLVNVEKMHAIAEKVRTVRKYRSNQLRLDTDVSPTHLQNKAYVRQFQVIDNQNLLFELSCKLEPNTQ

Target ORF information:

RefSeq Version	XM_012971530.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971530.2

ORF Insert Sequence:

ATGAAACCTC TGGAAAAATT CTTGAAAAAG CAAAGCTCTC ATCTTTCTGG ACCCCGACAC 
CTACACAGAA GGCAGAGCGT CTCCAAAATC CTGCCTTCTT TCCTGAGGGA TACTCCGGGG 
GACTCCTCTG GGGGAGAAAG GTGTGAGGAA GATCCATCAG GTGGGCCGCC GCCATCCCAG 
GACTGCCTGG AACTACCAGA TGGACTTCGG TCCCCTCTTT CTTTTTCTTC AGATGAACTA 
TCCCCCTCAG AACCCCTCAC ACCTCCGCCA GGATCTGGGG GCTGGACCCT TTCCCCTCCT 
GGCCCTCTGC TTGCCCCTGA TACCCCTGAG GCTCTACTCG GGAGGGTCCT GGAGCAGCTA 
CTGAGTGCGC CGAGGCTAAA TGAAGCAGCA GAATTGGCCC TGGACGACTT TATCCTCACG 
CATACGCTAT TTATGCCGAC AGAGGAGCTC CTCCTCCAAT TACAGCAGTA CTTCTGCGGC 
TGCAGCCGAT ATACTACTCC GGCGTGGGAA GGGAGCGACC CTCTTCAGAG GAAGCAGGCT 
GTACTCTCAT CTCTTTTACA CCTCTTGGAT ATTTACAAGG ACGCTCTCCA GGAGGAGGAG 
AGGACATTCC AGCTAATCAA GGATTTCTAT GTCTTGGTGA TGAGGGATGC CCCGAATTTC 
CCGCAGCTGG AGGGGAGCGT CATCCGATTG CACAGGCTTG TGGAAAGCGC TGAGCTCAGG 
CTTACAGATG GCAGTGCCAC TCAATGCTCA AAGCAAGTCA AACCTCTCTT CCGCCATTTC 
CGAAGAATCG ACTCCTGCCT GCAGCCACGG GTGGCATTCA GGGGCTCTGA CGAGATTTTT 
TGCAGGGTCT ACATGCCTGA TCATTCGTAC GTTACAATTA GGAGCCGACT TTCTGCTTCT 
GTCTCTGATA TCCTGACCTC TGTTTCTGAG AAACTGCAAT ATTCAGAAGA ACAGGGGCAG 
AGAGAGGAAC CCCTGATCCT GGTGGCTGTG ACAAGTGCTG GAGACAAGGT TCTGCTGAAG 
CCAGATGATG GCTGTATCTT TACCACTCTG GGGATTAATA GTCACTTGTT CGCCTGCAAT 
CGGGAGGAGC TGCGCTCTTT GGTTCCCCTT CCAGAGGAAG TTCAACTGCC TCCCGAAGAC 
TCCCATATTC ACAGGATTGA GGCAGAAGAC TTGGCCAATC AACTGAGTGC TTTCCACTGG 
GAACTTTTCA GCTGTGTGCA TGAGCTGGAA TTTATAGACT ATGTGTTCCA CGTGGAGCGA 
GGACGCAGGG AGACGGCGAA TCTGGAACTT TTGTTGCAGA GGTGCAGTGA GGTGCAGCAC 
TGGGTCAGTA CTCAGACTCT GCTCTGCGAG GGGCCAGCAC GCCGCACACA ACTTCTCAAG 
AAATTCATCA AGACAGCTGC TATCTGTAAA CAAAACCAGG ACCTGCTGTC ATTCTTTGCA 
ATCGTGATGG GCTTGGATAA TGGAGCTGTG AGCCGCCTTC GGGTGACATG GGAAGGTACT 
ATAGACAGGG CAAGAAATGC TCGCTGTCAG AAATTGCCAG GAAAATGTAA GAACTTGTTC 
CGAAAGTTTG AGAATTTGAC GGATCCGTGC CGGAACCACA AATCCTACCG TGAGATGGTG 
TCTCGTATGC GCCCTCCTGT AATTCCATTT ATACCCCTTA TTCTGAAAGA TCTCACTTTT 
TTACACGAGG GCAGCAAAAC CTTCATTGAC GGCCTTGTGA ATGTAGAAAA AATGCACGCC 
ATTGCTGAAA AAGTGAGGAC TGTGCGAAAA TACCGGAGTA ACCAGCTACG GCTGGATACC 
GATGTCTCCC CGACTCATCT GCAAAATAAA GCCTACGTGC GCCAGTTCCA AGTTATCGAC 
AACCAGAACC TGCTGTTTGA GTTGTCCTGC AAACTGGAGC CCAATACGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgefl1 ( XM_018089428.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944917.1 Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa32039
Clone ID Related Accession (Same CDS sequence)	XM_018089428.1
Accession Version	XM_018089428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor-like 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683386.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCTC TGGAAAAATT CTTGAAAAAG CAAAGCTCTC ATCTTTCTGG ACCCCGACAC 
CTACACAGAA GGCAGAGCGT CTCCAAAATC CTGCCTTCTT TCCTGAGGGA TACTCCGGGG 
GACTCCTCTG GGGGAGAAAG GTGTGAGGAA GATCCATCAG GTGGGCCGCC GCCATCCCAG 
GACTGCCTGG AACTACCAGA TGGACTTCGG TCCCCTCTTT CTTTTTCTTC AGATGAACTA 
TCCCCCTCAG AACCCCTCAC ACCTCCGCCA GGATCTGGGG GCTGGACCCT TTCCCCTCCT 
GGCCCTCTGC TTGCCCCTGA TACCCCTGAG GCTCTACTCG GGAGGGTCCT GGAGCAGCTA 
CTGAGTGCGC CGAGGCTAAA TGAAGCAGCA GAATTGGCCC TGGACGACTT TATCCTCACG 
CATACGCTAT TTATGCCGAC AGAGGAGCTC CTCCTCCAAT TACAGCAGTA CTTCTGCGGC 
TGCAGCCGAT ATACTACTCC GGCGTGGGAA GGGAGCGACC CTCTTCAGAG GAAGCAGGCT 
GTACTCTCAT CTCTTTTACA CCTCTTGGAT ATTTACAAGG ACGCTCTCCA GGAGGAGGAG 
AGGACATTCC AGCTAATCAA GGATTTCTAT GTCTTGGTGA TGAGGGATGC CCCGAATTTC 
CCGCAGCTGG AGGGGAGCGT CATCCGATTG CACAGGCTTG TGGAAAGCGC TGAGCTCAGG 
CTTACAGATG GCAGTGCCAC TCAATGCTCA AAGCAAGTCA AACCTCTCTT CCGCCATTTC 
CGAAGAATCG ACTCCTGCCT GCAGCCACGG GTGGCATTCA GGGGCTCTGA CGAGATTTTT 
TGCAGGGTCT ACATGCCTGA TCATTCGTAC GTTACAATTA GGAGCCGACT TTCTGCTTCT 
GTCTCTGATA TCCTGACCTC TGTTTCTGAG AAACTGCAAT ATTCAGAAGA ACAGGGGCAG 
AGAGAGGAAC CCCTGATCCT GGTGGCTGTG ACAAGTGCTG GAGACAAGGT TCTGCTGAAG 
CCAGATGATG GCTGTATCTT TACCACTCTG GGGATTAATA GTCACTTGTT CGCCTGCAAT 
CGGGAGGAGC TGCGCTCTTT GGTTCCCCTT CCAGAGGAAG TTCAACTGCC TCCCGAAGAC 
TCCCATATTC ACAGGATTGA GGCAGAAGAC TTGGCCAATC AACTGAGTGC TTTCCACTGG 
GAACTTTTCA GCTGTGTGCA TGAGCTGGAA TTTATAGACT ATGTGTTCCA CGTGGAGCGA 
GGACGCAGGG AGACGGCGAA TCTGGAACTT TTGTTGCAGA GGTGCAGTGA GGTGCAGCAC 
TGGGTCAGTA CTCAGACTCT GCTCTGCGAG GGGCCAGCAC GCCGCACACA ACTTCTCAAG 
AAATTCATCA AGACAGCTGC TATCTGTAAA CAAAACCAGG ACCTGCTGTC ATTCTTTGCA 
ATCGTGATGG GCTTGGATAA TGGAGCTGTG AGCCGCCTTC GGGTGACATG GGAAAAATTG 
CCAGGAAAAT GTAAGAACTT GTTCCGAAAG TTTGAGAATT TGACGGATCC GTGCCGGAAC 
CACAAATCCT ACCGTGAGAT GGTGTCTCGT ATGCGCCCTC CTGTAATTCC ATTTATACCC 
CTTATTCTGA AAGATCTCAC TTTTTTACAC GAGGGCAGCA AAACCTTCAT TGACGGCCTT 
GTGAATGTAG AAAAAATGCA CGCCATTGCT GAAAAAGTGA GGACTGTGCG AAAATACCGG 
AGTAACCAGC TACGGCTGGA TACCGATGTC TCCCCGACTC ATCTGCAAAA TAAAGCCTAC 
GTGCGCCAGT TCCAAGTTAT CGACAACCAG AACCTGCTGT TTGAGTTGTC CTGCAAACTG 
GAGCCCAATA CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944917.1
CDS	1674..3551
Translation	MKPLEKFLKKQSSHLSGPRHLHRRQSVSKILPSFLRDTPGDSSGGERCEEDPSGGPPPSQDCLELPDGLRSPLSFSSDELSPSEPLTPPPGSGGWTLSPPGPLLAPDTPEALLGRVLEQLLSAPRLNEAAELALDDFILTHTLFMPTEELLLQLQQYFCGCSRYTTPAWEGSDPLQRKQAVLSSLLHLLDIYKDALQEEERTFQLIKDFYVLVMRDAPNFPQLEGSVIRLHRLVESAELRLTDGSATQCSKQVKPLFRHFRRIDSCLQPRVAFRGSDEIFCRVYMPDHSYVTIRSRLSASVSDILTSVSEKLQYSEEQGQREEPLILVAVTSAGDKVLLKPDDGCIFTTLGINSHLFACNREELRSLVPLPEEVQLPPEDSHIHRIEAEDLANQLSAFHWELFSCVHELEFIDYVFHVERGRRETANLELLLQRCSEVQHWVSTQTLLCEGPARRTQLLKKFIKTAAICKQNQDLLSFFAIVMGLDNGAVSRLRVTWEKLPGKCKNLFRKFENLTDPCRNHKSYREMVSRMRPPVIPFIPLILKDLTFLHEGSKTFIDGLVNVEKMHAIAEKVRTVRKYRSNQLRLDTDVSPTHLQNKAYVRQFQVIDNQNLLFELSCKLEPNTQ

Target ORF information:

RefSeq Version	XM_018089428.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089428.1

ORF Insert Sequence:

ATGAAACCTC TGGAAAAATT CTTGAAAAAG CAAAGCTCTC ATCTTTCTGG ACCCCGACAC 
CTACACAGAA GGCAGAGCGT CTCCAAAATC CTGCCTTCTT TCCTGAGGGA TACTCCGGGG 
GACTCCTCTG GGGGAGAAAG GTGTGAGGAA GATCCATCAG GTGGGCCGCC GCCATCCCAG 
GACTGCCTGG AACTACCAGA TGGACTTCGG TCCCCTCTTT CTTTTTCTTC AGATGAACTA 
TCCCCCTCAG AACCCCTCAC ACCTCCGCCA GGATCTGGGG GCTGGACCCT TTCCCCTCCT 
GGCCCTCTGC TTGCCCCTGA TACCCCTGAG GCTCTACTCG GGAGGGTCCT GGAGCAGCTA 
CTGAGTGCGC CGAGGCTAAA TGAAGCAGCA GAATTGGCCC TGGACGACTT TATCCTCACG 
CATACGCTAT TTATGCCGAC AGAGGAGCTC CTCCTCCAAT TACAGCAGTA CTTCTGCGGC 
TGCAGCCGAT ATACTACTCC GGCGTGGGAA GGGAGCGACC CTCTTCAGAG GAAGCAGGCT 
GTACTCTCAT CTCTTTTACA CCTCTTGGAT ATTTACAAGG ACGCTCTCCA GGAGGAGGAG 
AGGACATTCC AGCTAATCAA GGATTTCTAT GTCTTGGTGA TGAGGGATGC CCCGAATTTC 
CCGCAGCTGG AGGGGAGCGT CATCCGATTG CACAGGCTTG TGGAAAGCGC TGAGCTCAGG 
CTTACAGATG GCAGTGCCAC TCAATGCTCA AAGCAAGTCA AACCTCTCTT CCGCCATTTC 
CGAAGAATCG ACTCCTGCCT GCAGCCACGG GTGGCATTCA GGGGCTCTGA CGAGATTTTT 
TGCAGGGTCT ACATGCCTGA TCATTCGTAC GTTACAATTA GGAGCCGACT TTCTGCTTCT 
GTCTCTGATA TCCTGACCTC TGTTTCTGAG AAACTGCAAT ATTCAGAAGA ACAGGGGCAG 
AGAGAGGAAC CCCTGATCCT GGTGGCTGTG ACAAGTGCTG GAGACAAGGT TCTGCTGAAG 
CCAGATGATG GCTGTATCTT TACCACTCTG GGGATTAATA GTCACTTGTT CGCCTGCAAT 
CGGGAGGAGC TGCGCTCTTT GGTTCCCCTT CCAGAGGAAG TTCAACTGCC TCCCGAAGAC 
TCCCATATTC ACAGGATTGA GGCAGAAGAC TTGGCCAATC AACTGAGTGC TTTCCACTGG 
GAACTTTTCA GCTGTGTGCA TGAGCTGGAA TTTATAGACT ATGTGTTCCA CGTGGAGCGA 
GGACGCAGGG AGACGGCGAA TCTGGAACTT TTGTTGCAGA GGTGCAGTGA GGTGCAGCAC 
TGGGTCAGTA CTCAGACTCT GCTCTGCGAG GGGCCAGCAC GCCGCACACA ACTTCTCAAG 
AAATTCATCA AGACAGCTGC TATCTGTAAA CAAAACCAGG ACCTGCTGTC ATTCTTTGCA 
ATCGTGATGG GCTTGGATAA TGGAGCTGTG AGCCGCCTTC GGGTGACATG GGAAAAATTG 
CCAGGAAAAT GTAAGAACTT GTTCCGAAAG TTTGAGAATT TGACGGATCC GTGCCGGAAC 
CACAAATCCT ACCGTGAGAT GGTGTCTCGT ATGCGCCCTC CTGTAATTCC ATTTATACCC 
CTTATTCTGA AAGATCTCAC TTTTTTACAC GAGGGCAGCA AAACCTTCAT TGACGGCCTT 
GTGAATGTAG AAAAAATGCA CGCCATTGCT GAAAAAGTGA GGACTGTGCG AAAATACCGG 
AGTAACCAGC TACGGCTGGA TACCGATGTC TCCCCGACTC ATCTGCAAAA TAAAGCCTAC 
GTGCGCCAGT TCCAAGTTAT CGACAACCAG AACCTGCTGT TTGAGTTGTC CTGCAAACTG 
GAGCCCAATA CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgefl1 ( XM_031893985.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031749845.1 Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa49965
Clone ID Related Accession (Same CDS sequence)	XM_031893985.1 , XM_012971530.3
Accession Version	XM_031893985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor-like 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCTC TGGAAAAATT CTTGAAAAAG CAAAGCTCTC ATCTTTCTGG ACCCCGACAC 
CTACACAGAA GGCAGAGCGT CTCCAAAATC CTGCCTTCTT TCCTGAGGGA TACTCCGGGG 
GACTCCTCTG GGGGAGAAAG GTGTGAGGAA GATCCATCAG GTGGGCCGCC GCCATCCCAG 
GACTGCCTGG AACTACCAGA TGGACTTCGG TCCCCTCTTT CTTTTTCTTC AGATGAACTA 
TCCCCCTCAG AACCCCTCAC ACCTCCGCCA GGATCTGGGG GCTGGACCCT TTCTCCTCCT 
GGCCCTCTGC TTGCCCCTGA TACCCCTGAG GCTCTACTCG GGAGGGTCCT GGAGCAGCTA 
CTGAGTGCGC CGAGGCTAAA TGAAGCAGCA GAATTGGCCC TGGACGACTT TATCCTCACG 
CATACGCTAT TTATGCCGAC AGAGGAGCTC CTCCTCCAAT TACAGCAGTA CTTCTGCGGC 
TGCAGCCGAT ATACTACTCC GGCGTGGGAA GGGAGCGACC CTCTTCAGAG GAAGCAGGCT 
GTACTCTCAT CTCTTTTACA CCTCTTGGAT ATTTACAAGG ACGCTCTCCA GGAGGAGGAG 
AGGACATTCC AGCTAATCAA GGATTTCTAT GTCTTGGTGA TGAGGGATGC CCCGAATTTC 
CCACAGCTGG AGGGGAGCGT CATCCGATTG CACAGGCTTG TGGAAAGCGC TGAGCTCAGG 
CTTACAGATG GCAGTGCCAC TCAATGCTCA AAGCAAGTCA AACCTCTCTT CCGCCATTTC 
CGAAGAATCG ACTCCTGCCT GCAGCCACGG GTGGCATTCA GGGGCTCTGA CGAGATTTTT 
TGCAGGGTCT ACATGCCTGA TCATTCGTAC GTTACAATTA GGAGCCGACT TTCTGCTTCT 
GTCTCTGATA TCCTGACCTC TGTTTCTGAG AAACTGCAAT ATTCAGAAGA ACAGGGGCAG 
AGAGAGGAAC CCCTGATCCT GGTGGCTGTG ACAAGTGCTG GAGACAAGGT TCTGCTGAAG 
CCAGATGATG GCTGTATCTT TACCACTCTG GGGATTAATA GTCACTTGTT CGCCTGCAAT 
CGGGAGGAGC TGCGCTCTTT GGTTCCCCTT CCAGAGGAAG TTCAACTGCC TCCCGAAGAC 
TCCCATATTC ACAGGATTGA GGCAGAAGAC TTGGCCAATC AACTGAGTGC TTTCCACTGG 
GAACTTTTCA GCTGTGTGCA TGAGCTGGAA TTTATAGACT ATGTGTTCCA CGTGGAGCGA 
GGACGCAGGG AGACGGCGAA TCTGGAACTT TTGTTGCAGA GGTGCAGTGA GGTGCAGCAC 
TGGGTCAGTA CTCAGACTCT GCTCTGCGAG GGGCCAGCAC GCCGCACACA ACTTCTCAAG 
AAATTCATCA AGACAGCTGC TATCTGTAAA CAAAACCAGG ACCTGCTGTC ATTCTTTGCA 
ATCGTGATGG GCTTGGATAA TGGAGCTGTG AGCCGCCTTC GGGTGACATG GGAAGGTACT 
ATAGACAGGG CAAGAAATGC TCGCTGTCAG AAATTGCCAG GAAAATGTAA GAACTTGTTC 
CGAAAGTTTG AGAATTTGAC GGATCCGTGC CGGAACCACA AATCCTACCG TGAGATGGTG 
TCTCGTATGC GCCCTCCTGT AATTCCATTT ATACCCCTTA TTCTGAAAGA TCTCACTTTT 
TTACACGAGG GCAGCAAAAC CTTCATTGAC GGCCTTGTGA ATGTAGAAAA AATGCACGCC 
ATTGCTGAAA AAGTGAGGAC TGTGCGAAAA TACCGGAGTA ACCAGCTACG GCTGGATACC 
GATGTCTCCC CGACTCATCT GCAAAATAAA GCCTACGTGC GCCAGTTCCA AGTTATCGAC 
AACCAGAACC TGCTGTTTGA GTTGTCCTGC AAACTGGAGC CCAATACGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031749845.1
CDS	1499..3412
Translation	MKPLEKFLKKQSSHLSGPRHLHRRQSVSKILPSFLRDTPGDSSGGERCEEDPSGGPPPSQDCLELPDGLRSPLSFSSDELSPSEPLTPPPGSGGWTLSPPGPLLAPDTPEALLGRVLEQLLSAPRLNEAAELALDDFILTHTLFMPTEELLLQLQQYFCGCSRYTTPAWEGSDPLQRKQAVLSSLLHLLDIYKDALQEEERTFQLIKDFYVLVMRDAPNFPQLEGSVIRLHRLVESAELRLTDGSATQCSKQVKPLFRHFRRIDSCLQPRVAFRGSDEIFCRVYMPDHSYVTIRSRLSASVSDILTSVSEKLQYSEEQGQREEPLILVAVTSAGDKVLLKPDDGCIFTTLGINSHLFACNREELRSLVPLPEEVQLPPEDSHIHRIEAEDLANQLSAFHWELFSCVHELEFIDYVFHVERGRRETANLELLLQRCSEVQHWVSTQTLLCEGPARRTQLLKKFIKTAAICKQNQDLLSFFAIVMGLDNGAVSRLRVTWEGTIDRARNARCQKLPGKCKNLFRKFENLTDPCRNHKSYREMVSRMRPPVIPFIPLILKDLTFLHEGSKTFIDGLVNVEKMHAIAEKVRTVRKYRSNQLRLDTDVSPTHLQNKAYVRQFQVIDNQNLLFELSCKLEPNTQ

Target ORF information:

RefSeq Version	XM_031893985.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031893985.1

ORF Insert Sequence:

ATGAAACCTC TGGAAAAATT CTTGAAAAAG CAAAGCTCTC ATCTTTCTGG ACCCCGACAC 
CTACACAGAA GGCAGAGCGT CTCCAAAATC CTGCCTTCTT TCCTGAGGGA TACTCCGGGG 
GACTCCTCTG GGGGAGAAAG GTGTGAGGAA GATCCATCAG GTGGGCCGCC GCCATCCCAG 
GACTGCCTGG AACTACCAGA TGGACTTCGG TCCCCTCTTT CTTTTTCTTC AGATGAACTA 
TCCCCCTCAG AACCCCTCAC ACCTCCGCCA GGATCTGGGG GCTGGACCCT TTCTCCTCCT 
GGCCCTCTGC TTGCCCCTGA TACCCCTGAG GCTCTACTCG GGAGGGTCCT GGAGCAGCTA 
CTGAGTGCGC CGAGGCTAAA TGAAGCAGCA GAATTGGCCC TGGACGACTT TATCCTCACG 
CATACGCTAT TTATGCCGAC AGAGGAGCTC CTCCTCCAAT TACAGCAGTA CTTCTGCGGC 
TGCAGCCGAT ATACTACTCC GGCGTGGGAA GGGAGCGACC CTCTTCAGAG GAAGCAGGCT 
GTACTCTCAT CTCTTTTACA CCTCTTGGAT ATTTACAAGG ACGCTCTCCA GGAGGAGGAG 
AGGACATTCC AGCTAATCAA GGATTTCTAT GTCTTGGTGA TGAGGGATGC CCCGAATTTC 
CCACAGCTGG AGGGGAGCGT CATCCGATTG CACAGGCTTG TGGAAAGCGC TGAGCTCAGG 
CTTACAGATG GCAGTGCCAC TCAATGCTCA AAGCAAGTCA AACCTCTCTT CCGCCATTTC 
CGAAGAATCG ACTCCTGCCT GCAGCCACGG GTGGCATTCA GGGGCTCTGA CGAGATTTTT 
TGCAGGGTCT ACATGCCTGA TCATTCGTAC GTTACAATTA GGAGCCGACT TTCTGCTTCT 
GTCTCTGATA TCCTGACCTC TGTTTCTGAG AAACTGCAAT ATTCAGAAGA ACAGGGGCAG 
AGAGAGGAAC CCCTGATCCT GGTGGCTGTG ACAAGTGCTG GAGACAAGGT TCTGCTGAAG 
CCAGATGATG GCTGTATCTT TACCACTCTG GGGATTAATA GTCACTTGTT CGCCTGCAAT 
CGGGAGGAGC TGCGCTCTTT GGTTCCCCTT CCAGAGGAAG TTCAACTGCC TCCCGAAGAC 
TCCCATATTC ACAGGATTGA GGCAGAAGAC TTGGCCAATC AACTGAGTGC TTTCCACTGG 
GAACTTTTCA GCTGTGTGCA TGAGCTGGAA TTTATAGACT ATGTGTTCCA CGTGGAGCGA 
GGACGCAGGG AGACGGCGAA TCTGGAACTT TTGTTGCAGA GGTGCAGTGA GGTGCAGCAC 
TGGGTCAGTA CTCAGACTCT GCTCTGCGAG GGGCCAGCAC GCCGCACACA ACTTCTCAAG 
AAATTCATCA AGACAGCTGC TATCTGTAAA CAAAACCAGG ACCTGCTGTC ATTCTTTGCA 
ATCGTGATGG GCTTGGATAA TGGAGCTGTG AGCCGCCTTC GGGTGACATG GGAAGGTACT 
ATAGACAGGG CAAGAAATGC TCGCTGTCAG AAATTGCCAG GAAAATGTAA GAACTTGTTC 
CGAAAGTTTG AGAATTTGAC GGATCCGTGC CGGAACCACA AATCCTACCG TGAGATGGTG 
TCTCGTATGC GCCCTCCTGT AATTCCATTT ATACCCCTTA TTCTGAAAGA TCTCACTTTT 
TTACACGAGG GCAGCAAAAC CTTCATTGAC GGCCTTGTGA ATGTAGAAAA AATGCACGCC 
ATTGCTGAAA AAGTGAGGAC TGTGCGAAAA TACCGGAGTA ACCAGCTACG GCTGGATACC 
GATGTCTCCC CGACTCATCT GCAAAATAAA GCCTACGTGC GCCAGTTCCA AGTTATCGAC 
AACCAGAACC TGCTGTTTGA GTTGTCCTGC AAACTGGAGC CCAATACGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgefl1 ( XM_012971530.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826984.1 Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa49965
Clone ID Related Accession (Same CDS sequence)	XM_031893985.1 , XM_012971530.3
Accession Version	XM_012971530.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor-like 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971530.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCTC TGGAAAAATT CTTGAAAAAG CAAAGCTCTC ATCTTTCTGG ACCCCGACAC 
CTACACAGAA GGCAGAGCGT CTCCAAAATC CTGCCTTCTT TCCTGAGGGA TACTCCGGGG 
GACTCCTCTG GGGGAGAAAG GTGTGAGGAA GATCCATCAG GTGGGCCGCC GCCATCCCAG 
GACTGCCTGG AACTACCAGA TGGACTTCGG TCCCCTCTTT CTTTTTCTTC AGATGAACTA 
TCCCCCTCAG AACCCCTCAC ACCTCCGCCA GGATCTGGGG GCTGGACCCT TTCTCCTCCT 
GGCCCTCTGC TTGCCCCTGA TACCCCTGAG GCTCTACTCG GGAGGGTCCT GGAGCAGCTA 
CTGAGTGCGC CGAGGCTAAA TGAAGCAGCA GAATTGGCCC TGGACGACTT TATCCTCACG 
CATACGCTAT TTATGCCGAC AGAGGAGCTC CTCCTCCAAT TACAGCAGTA CTTCTGCGGC 
TGCAGCCGAT ATACTACTCC GGCGTGGGAA GGGAGCGACC CTCTTCAGAG GAAGCAGGCT 
GTACTCTCAT CTCTTTTACA CCTCTTGGAT ATTTACAAGG ACGCTCTCCA GGAGGAGGAG 
AGGACATTCC AGCTAATCAA GGATTTCTAT GTCTTGGTGA TGAGGGATGC CCCGAATTTC 
CCACAGCTGG AGGGGAGCGT CATCCGATTG CACAGGCTTG TGGAAAGCGC TGAGCTCAGG 
CTTACAGATG GCAGTGCCAC TCAATGCTCA AAGCAAGTCA AACCTCTCTT CCGCCATTTC 
CGAAGAATCG ACTCCTGCCT GCAGCCACGG GTGGCATTCA GGGGCTCTGA CGAGATTTTT 
TGCAGGGTCT ACATGCCTGA TCATTCGTAC GTTACAATTA GGAGCCGACT TTCTGCTTCT 
GTCTCTGATA TCCTGACCTC TGTTTCTGAG AAACTGCAAT ATTCAGAAGA ACAGGGGCAG 
AGAGAGGAAC CCCTGATCCT GGTGGCTGTG ACAAGTGCTG GAGACAAGGT TCTGCTGAAG 
CCAGATGATG GCTGTATCTT TACCACTCTG GGGATTAATA GTCACTTGTT CGCCTGCAAT 
CGGGAGGAGC TGCGCTCTTT GGTTCCCCTT CCAGAGGAAG TTCAACTGCC TCCCGAAGAC 
TCCCATATTC ACAGGATTGA GGCAGAAGAC TTGGCCAATC AACTGAGTGC TTTCCACTGG 
GAACTTTTCA GCTGTGTGCA TGAGCTGGAA TTTATAGACT ATGTGTTCCA CGTGGAGCGA 
GGACGCAGGG AGACGGCGAA TCTGGAACTT TTGTTGCAGA GGTGCAGTGA GGTGCAGCAC 
TGGGTCAGTA CTCAGACTCT GCTCTGCGAG GGGCCAGCAC GCCGCACACA ACTTCTCAAG 
AAATTCATCA AGACAGCTGC TATCTGTAAA CAAAACCAGG ACCTGCTGTC ATTCTTTGCA 
ATCGTGATGG GCTTGGATAA TGGAGCTGTG AGCCGCCTTC GGGTGACATG GGAAGGTACT 
ATAGACAGGG CAAGAAATGC TCGCTGTCAG AAATTGCCAG GAAAATGTAA GAACTTGTTC 
CGAAAGTTTG AGAATTTGAC GGATCCGTGC CGGAACCACA AATCCTACCG TGAGATGGTG 
TCTCGTATGC GCCCTCCTGT AATTCCATTT ATACCCCTTA TTCTGAAAGA TCTCACTTTT 
TTACACGAGG GCAGCAAAAC CTTCATTGAC GGCCTTGTGA ATGTAGAAAA AATGCACGCC 
ATTGCTGAAA AAGTGAGGAC TGTGCGAAAA TACCGGAGTA ACCAGCTACG GCTGGATACC 
GATGTCTCCC CGACTCATCT GCAAAATAAA GCCTACGTGC GCCAGTTCCA AGTTATCGAC 
AACCAGAACC TGCTGTTTGA GTTGTCCTGC AAACTGGAGC CCAATACGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826984.1
CDS	629..2542
Translation	MKPLEKFLKKQSSHLSGPRHLHRRQSVSKILPSFLRDTPGDSSGGERCEEDPSGGPPPSQDCLELPDGLRSPLSFSSDELSPSEPLTPPPGSGGWTLSPPGPLLAPDTPEALLGRVLEQLLSAPRLNEAAELALDDFILTHTLFMPTEELLLQLQQYFCGCSRYTTPAWEGSDPLQRKQAVLSSLLHLLDIYKDALQEEERTFQLIKDFYVLVMRDAPNFPQLEGSVIRLHRLVESAELRLTDGSATQCSKQVKPLFRHFRRIDSCLQPRVAFRGSDEIFCRVYMPDHSYVTIRSRLSASVSDILTSVSEKLQYSEEQGQREEPLILVAVTSAGDKVLLKPDDGCIFTTLGINSHLFACNREELRSLVPLPEEVQLPPEDSHIHRIEAEDLANQLSAFHWELFSCVHELEFIDYVFHVERGRRETANLELLLQRCSEVQHWVSTQTLLCEGPARRTQLLKKFIKTAAICKQNQDLLSFFAIVMGLDNGAVSRLRVTWEGTIDRARNARCQKLPGKCKNLFRKFENLTDPCRNHKSYREMVSRMRPPVIPFIPLILKDLTFLHEGSKTFIDGLVNVEKMHAIAEKVRTVRKYRSNQLRLDTDVSPTHLQNKAYVRQFQVIDNQNLLFELSCKLEPNTQ

Target ORF information:

RefSeq Version	XM_012971530.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971530.3

ORF Insert Sequence:

ATGAAACCTC TGGAAAAATT CTTGAAAAAG CAAAGCTCTC ATCTTTCTGG ACCCCGACAC 
CTACACAGAA GGCAGAGCGT CTCCAAAATC CTGCCTTCTT TCCTGAGGGA TACTCCGGGG 
GACTCCTCTG GGGGAGAAAG GTGTGAGGAA GATCCATCAG GTGGGCCGCC GCCATCCCAG 
GACTGCCTGG AACTACCAGA TGGACTTCGG TCCCCTCTTT CTTTTTCTTC AGATGAACTA 
TCCCCCTCAG AACCCCTCAC ACCTCCGCCA GGATCTGGGG GCTGGACCCT TTCTCCTCCT 
GGCCCTCTGC TTGCCCCTGA TACCCCTGAG GCTCTACTCG GGAGGGTCCT GGAGCAGCTA 
CTGAGTGCGC CGAGGCTAAA TGAAGCAGCA GAATTGGCCC TGGACGACTT TATCCTCACG 
CATACGCTAT TTATGCCGAC AGAGGAGCTC CTCCTCCAAT TACAGCAGTA CTTCTGCGGC 
TGCAGCCGAT ATACTACTCC GGCGTGGGAA GGGAGCGACC CTCTTCAGAG GAAGCAGGCT 
GTACTCTCAT CTCTTTTACA CCTCTTGGAT ATTTACAAGG ACGCTCTCCA GGAGGAGGAG 
AGGACATTCC AGCTAATCAA GGATTTCTAT GTCTTGGTGA TGAGGGATGC CCCGAATTTC 
CCACAGCTGG AGGGGAGCGT CATCCGATTG CACAGGCTTG TGGAAAGCGC TGAGCTCAGG 
CTTACAGATG GCAGTGCCAC TCAATGCTCA AAGCAAGTCA AACCTCTCTT CCGCCATTTC 
CGAAGAATCG ACTCCTGCCT GCAGCCACGG GTGGCATTCA GGGGCTCTGA CGAGATTTTT 
TGCAGGGTCT ACATGCCTGA TCATTCGTAC GTTACAATTA GGAGCCGACT TTCTGCTTCT 
GTCTCTGATA TCCTGACCTC TGTTTCTGAG AAACTGCAAT ATTCAGAAGA ACAGGGGCAG 
AGAGAGGAAC CCCTGATCCT GGTGGCTGTG ACAAGTGCTG GAGACAAGGT TCTGCTGAAG 
CCAGATGATG GCTGTATCTT TACCACTCTG GGGATTAATA GTCACTTGTT CGCCTGCAAT 
CGGGAGGAGC TGCGCTCTTT GGTTCCCCTT CCAGAGGAAG TTCAACTGCC TCCCGAAGAC 
TCCCATATTC ACAGGATTGA GGCAGAAGAC TTGGCCAATC AACTGAGTGC TTTCCACTGG 
GAACTTTTCA GCTGTGTGCA TGAGCTGGAA TTTATAGACT ATGTGTTCCA CGTGGAGCGA 
GGACGCAGGG AGACGGCGAA TCTGGAACTT TTGTTGCAGA GGTGCAGTGA GGTGCAGCAC 
TGGGTCAGTA CTCAGACTCT GCTCTGCGAG GGGCCAGCAC GCCGCACACA ACTTCTCAAG 
AAATTCATCA AGACAGCTGC TATCTGTAAA CAAAACCAGG ACCTGCTGTC ATTCTTTGCA 
ATCGTGATGG GCTTGGATAA TGGAGCTGTG AGCCGCCTTC GGGTGACATG GGAAGGTACT 
ATAGACAGGG CAAGAAATGC TCGCTGTCAG AAATTGCCAG GAAAATGTAA GAACTTGTTC 
CGAAAGTTTG AGAATTTGAC GGATCCGTGC CGGAACCACA AATCCTACCG TGAGATGGTG 
TCTCGTATGC GCCCTCCTGT AATTCCATTT ATACCCCTTA TTCTGAAAGA TCTCACTTTT 
TTACACGAGG GCAGCAAAAC CTTCATTGAC GGCCTTGTGA ATGTAGAAAA AATGCACGCC 
ATTGCTGAAA AAGTGAGGAC TGTGCGAAAA TACCGGAGTA ACCAGCTACG GCTGGATACC 
GATGTCTCCC CGACTCATCT GCAAAATAAA GCCTACGTGC GCCAGTTCCA AGTTATCGAC 
AACCAGAACC TGCTGTTTGA GTTGTCCTGC AAACTGGAGC CCAATACGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgefl1 ( XM_018089428.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944917.1 Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X3, mRNA.

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CloneID	OXa49966
Clone ID Related Accession (Same CDS sequence)	XM_018089428.2 , XM_031893986.1
Accession Version	XM_018089428.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor-like 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018089428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCTC TGGAAAAATT CTTGAAAAAG CAAAGCTCTC ATCTTTCTGG ACCCCGACAC 
CTACACAGAA GGCAGAGCGT CTCCAAAATC CTGCCTTCTT TCCTGAGGGA TACTCCGGGG 
GACTCCTCTG GGGGAGAAAG GTGTGAGGAA GATCCATCAG GTGGGCCGCC GCCATCCCAG 
GACTGCCTGG AACTACCAGA TGGACTTCGG TCCCCTCTTT CTTTTTCTTC AGATGAACTA 
TCCCCCTCAG AACCCCTCAC ACCTCCGCCA GGATCTGGGG GCTGGACCCT TTCTCCTCCT 
GGCCCTCTGC TTGCCCCTGA TACCCCTGAG GCTCTACTCG GGAGGGTCCT GGAGCAGCTA 
CTGAGTGCGC CGAGGCTAAA TGAAGCAGCA GAATTGGCCC TGGACGACTT TATCCTCACG 
CATACGCTAT TTATGCCGAC AGAGGAGCTC CTCCTCCAAT TACAGCAGTA CTTCTGCGGC 
TGCAGCCGAT ATACTACTCC GGCGTGGGAA GGGAGCGACC CTCTTCAGAG GAAGCAGGCT 
GTACTCTCAT CTCTTTTACA CCTCTTGGAT ATTTACAAGG ACGCTCTCCA GGAGGAGGAG 
AGGACATTCC AGCTAATCAA GGATTTCTAT GTCTTGGTGA TGAGGGATGC CCCGAATTTC 
CCACAGCTGG AGGGGAGCGT CATCCGATTG CACAGGCTTG TGGAAAGCGC TGAGCTCAGG 
CTTACAGATG GCAGTGCCAC TCAATGCTCA AAGCAAGTCA AACCTCTCTT CCGCCATTTC 
CGAAGAATCG ACTCCTGCCT GCAGCCACGG GTGGCATTCA GGGGCTCTGA CGAGATTTTT 
TGCAGGGTCT ACATGCCTGA TCATTCGTAC GTTACAATTA GGAGCCGACT TTCTGCTTCT 
GTCTCTGATA TCCTGACCTC TGTTTCTGAG AAACTGCAAT ATTCAGAAGA ACAGGGGCAG 
AGAGAGGAAC CCCTGATCCT GGTGGCTGTG ACAAGTGCTG GAGACAAGGT TCTGCTGAAG 
CCAGATGATG GCTGTATCTT TACCACTCTG GGGATTAATA GTCACTTGTT CGCCTGCAAT 
CGGGAGGAGC TGCGCTCTTT GGTTCCCCTT CCAGAGGAAG TTCAACTGCC TCCCGAAGAC 
TCCCATATTC ACAGGATTGA GGCAGAAGAC TTGGCCAATC AACTGAGTGC TTTCCACTGG 
GAACTTTTCA GCTGTGTGCA TGAGCTGGAA TTTATAGACT ATGTGTTCCA CGTGGAGCGA 
GGACGCAGGG AGACGGCGAA TCTGGAACTT TTGTTGCAGA GGTGCAGTGA GGTGCAGCAC 
TGGGTCAGTA CTCAGACTCT GCTCTGCGAG GGGCCAGCAC GCCGCACACA ACTTCTCAAG 
AAATTCATCA AGACAGCTGC TATCTGTAAA CAAAACCAGG ACCTGCTGTC ATTCTTTGCA 
ATCGTGATGG GCTTGGATAA TGGAGCTGTG AGCCGCCTTC GGGTGACATG GGAAAAATTG 
CCAGGAAAAT GTAAGAACTT GTTCCGAAAG TTTGAGAATT TGACGGATCC GTGCCGGAAC 
CACAAATCCT ACCGTGAGAT GGTGTCTCGT ATGCGCCCTC CTGTAATTCC ATTTATACCC 
CTTATTCTGA AAGATCTCAC TTTTTTACAC GAGGGCAGCA AAACCTTCAT TGACGGCCTT 
GTGAATGTAG AAAAAATGCA CGCCATTGCT GAAAAAGTGA GGACTGTGCG AAAATACCGG 
AGTAACCAGC TACGGCTGGA TACCGATGTC TCCCCGACTC ATCTGCAAAA TAAAGCCTAC 
GTGCGCCAGT TCCAAGTTAT CGACAACCAG AACCTGCTGT TTGAGTTGTC CTGCAAACTG 
GAGCCCAATA CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944917.1
CDS	1498..3375
Translation	MKPLEKFLKKQSSHLSGPRHLHRRQSVSKILPSFLRDTPGDSSGGERCEEDPSGGPPPSQDCLELPDGLRSPLSFSSDELSPSEPLTPPPGSGGWTLSPPGPLLAPDTPEALLGRVLEQLLSAPRLNEAAELALDDFILTHTLFMPTEELLLQLQQYFCGCSRYTTPAWEGSDPLQRKQAVLSSLLHLLDIYKDALQEEERTFQLIKDFYVLVMRDAPNFPQLEGSVIRLHRLVESAELRLTDGSATQCSKQVKPLFRHFRRIDSCLQPRVAFRGSDEIFCRVYMPDHSYVTIRSRLSASVSDILTSVSEKLQYSEEQGQREEPLILVAVTSAGDKVLLKPDDGCIFTTLGINSHLFACNREELRSLVPLPEEVQLPPEDSHIHRIEAEDLANQLSAFHWELFSCVHELEFIDYVFHVERGRRETANLELLLQRCSEVQHWVSTQTLLCEGPARRTQLLKKFIKTAAICKQNQDLLSFFAIVMGLDNGAVSRLRVTWEKLPGKCKNLFRKFENLTDPCRNHKSYREMVSRMRPPVIPFIPLILKDLTFLHEGSKTFIDGLVNVEKMHAIAEKVRTVRKYRSNQLRLDTDVSPTHLQNKAYVRQFQVIDNQNLLFELSCKLEPNTQ

Target ORF information:

RefSeq Version	XM_018089428.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089428.2

ORF Insert Sequence:

ATGAAACCTC TGGAAAAATT CTTGAAAAAG CAAAGCTCTC ATCTTTCTGG ACCCCGACAC 
CTACACAGAA GGCAGAGCGT CTCCAAAATC CTGCCTTCTT TCCTGAGGGA TACTCCGGGG 
GACTCCTCTG GGGGAGAAAG GTGTGAGGAA GATCCATCAG GTGGGCCGCC GCCATCCCAG 
GACTGCCTGG AACTACCAGA TGGACTTCGG TCCCCTCTTT CTTTTTCTTC AGATGAACTA 
TCCCCCTCAG AACCCCTCAC ACCTCCGCCA GGATCTGGGG GCTGGACCCT TTCTCCTCCT 
GGCCCTCTGC TTGCCCCTGA TACCCCTGAG GCTCTACTCG GGAGGGTCCT GGAGCAGCTA 
CTGAGTGCGC CGAGGCTAAA TGAAGCAGCA GAATTGGCCC TGGACGACTT TATCCTCACG 
CATACGCTAT TTATGCCGAC AGAGGAGCTC CTCCTCCAAT TACAGCAGTA CTTCTGCGGC 
TGCAGCCGAT ATACTACTCC GGCGTGGGAA GGGAGCGACC CTCTTCAGAG GAAGCAGGCT 
GTACTCTCAT CTCTTTTACA CCTCTTGGAT ATTTACAAGG ACGCTCTCCA GGAGGAGGAG 
AGGACATTCC AGCTAATCAA GGATTTCTAT GTCTTGGTGA TGAGGGATGC CCCGAATTTC 
CCACAGCTGG AGGGGAGCGT CATCCGATTG CACAGGCTTG TGGAAAGCGC TGAGCTCAGG 
CTTACAGATG GCAGTGCCAC TCAATGCTCA AAGCAAGTCA AACCTCTCTT CCGCCATTTC 
CGAAGAATCG ACTCCTGCCT GCAGCCACGG GTGGCATTCA GGGGCTCTGA CGAGATTTTT 
TGCAGGGTCT ACATGCCTGA TCATTCGTAC GTTACAATTA GGAGCCGACT TTCTGCTTCT 
GTCTCTGATA TCCTGACCTC TGTTTCTGAG AAACTGCAAT ATTCAGAAGA ACAGGGGCAG 
AGAGAGGAAC CCCTGATCCT GGTGGCTGTG ACAAGTGCTG GAGACAAGGT TCTGCTGAAG 
CCAGATGATG GCTGTATCTT TACCACTCTG GGGATTAATA GTCACTTGTT CGCCTGCAAT 
CGGGAGGAGC TGCGCTCTTT GGTTCCCCTT CCAGAGGAAG TTCAACTGCC TCCCGAAGAC 
TCCCATATTC ACAGGATTGA GGCAGAAGAC TTGGCCAATC AACTGAGTGC TTTCCACTGG 
GAACTTTTCA GCTGTGTGCA TGAGCTGGAA TTTATAGACT ATGTGTTCCA CGTGGAGCGA 
GGACGCAGGG AGACGGCGAA TCTGGAACTT TTGTTGCAGA GGTGCAGTGA GGTGCAGCAC 
TGGGTCAGTA CTCAGACTCT GCTCTGCGAG GGGCCAGCAC GCCGCACACA ACTTCTCAAG 
AAATTCATCA AGACAGCTGC TATCTGTAAA CAAAACCAGG ACCTGCTGTC ATTCTTTGCA 
ATCGTGATGG GCTTGGATAA TGGAGCTGTG AGCCGCCTTC GGGTGACATG GGAAAAATTG 
CCAGGAAAAT GTAAGAACTT GTTCCGAAAG TTTGAGAATT TGACGGATCC GTGCCGGAAC 
CACAAATCCT ACCGTGAGAT GGTGTCTCGT ATGCGCCCTC CTGTAATTCC ATTTATACCC 
CTTATTCTGA AAGATCTCAC TTTTTTACAC GAGGGCAGCA AAACCTTCAT TGACGGCCTT 
GTGAATGTAG AAAAAATGCA CGCCATTGCT GAAAAAGTGA GGACTGTGCG AAAATACCGG 
AGTAACCAGC TACGGCTGGA TACCGATGTC TCCCCGACTC ATCTGCAAAA TAAAGCCTAC 
GTGCGCCAGT TCCAAGTTAT CGACAACCAG AACCTGCTGT TTGAGTTGTC CTGCAAACTG 
GAGCCCAATA CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgefl1 ( XM_031893986.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031749846.1 Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa49966
Clone ID Related Accession (Same CDS sequence)	XM_018089428.2 , XM_031893986.1
Accession Version	XM_031893986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor-like 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCTC TGGAAAAATT CTTGAAAAAG CAAAGCTCTC ATCTTTCTGG ACCCCGACAC 
CTACACAGAA GGCAGAGCGT CTCCAAAATC CTGCCTTCTT TCCTGAGGGA TACTCCGGGG 
GACTCCTCTG GGGGAGAAAG GTGTGAGGAA GATCCATCAG GTGGGCCGCC GCCATCCCAG 
GACTGCCTGG AACTACCAGA TGGACTTCGG TCCCCTCTTT CTTTTTCTTC AGATGAACTA 
TCCCCCTCAG AACCCCTCAC ACCTCCGCCA GGATCTGGGG GCTGGACCCT TTCTCCTCCT 
GGCCCTCTGC TTGCCCCTGA TACCCCTGAG GCTCTACTCG GGAGGGTCCT GGAGCAGCTA 
CTGAGTGCGC CGAGGCTAAA TGAAGCAGCA GAATTGGCCC TGGACGACTT TATCCTCACG 
CATACGCTAT TTATGCCGAC AGAGGAGCTC CTCCTCCAAT TACAGCAGTA CTTCTGCGGC 
TGCAGCCGAT ATACTACTCC GGCGTGGGAA GGGAGCGACC CTCTTCAGAG GAAGCAGGCT 
GTACTCTCAT CTCTTTTACA CCTCTTGGAT ATTTACAAGG ACGCTCTCCA GGAGGAGGAG 
AGGACATTCC AGCTAATCAA GGATTTCTAT GTCTTGGTGA TGAGGGATGC CCCGAATTTC 
CCACAGCTGG AGGGGAGCGT CATCCGATTG CACAGGCTTG TGGAAAGCGC TGAGCTCAGG 
CTTACAGATG GCAGTGCCAC TCAATGCTCA AAGCAAGTCA AACCTCTCTT CCGCCATTTC 
CGAAGAATCG ACTCCTGCCT GCAGCCACGG GTGGCATTCA GGGGCTCTGA CGAGATTTTT 
TGCAGGGTCT ACATGCCTGA TCATTCGTAC GTTACAATTA GGAGCCGACT TTCTGCTTCT 
GTCTCTGATA TCCTGACCTC TGTTTCTGAG AAACTGCAAT ATTCAGAAGA ACAGGGGCAG 
AGAGAGGAAC CCCTGATCCT GGTGGCTGTG ACAAGTGCTG GAGACAAGGT TCTGCTGAAG 
CCAGATGATG GCTGTATCTT TACCACTCTG GGGATTAATA GTCACTTGTT CGCCTGCAAT 
CGGGAGGAGC TGCGCTCTTT GGTTCCCCTT CCAGAGGAAG TTCAACTGCC TCCCGAAGAC 
TCCCATATTC ACAGGATTGA GGCAGAAGAC TTGGCCAATC AACTGAGTGC TTTCCACTGG 
GAACTTTTCA GCTGTGTGCA TGAGCTGGAA TTTATAGACT ATGTGTTCCA CGTGGAGCGA 
GGACGCAGGG AGACGGCGAA TCTGGAACTT TTGTTGCAGA GGTGCAGTGA GGTGCAGCAC 
TGGGTCAGTA CTCAGACTCT GCTCTGCGAG GGGCCAGCAC GCCGCACACA ACTTCTCAAG 
AAATTCATCA AGACAGCTGC TATCTGTAAA CAAAACCAGG ACCTGCTGTC ATTCTTTGCA 
ATCGTGATGG GCTTGGATAA TGGAGCTGTG AGCCGCCTTC GGGTGACATG GGAAAAATTG 
CCAGGAAAAT GTAAGAACTT GTTCCGAAAG TTTGAGAATT TGACGGATCC GTGCCGGAAC 
CACAAATCCT ACCGTGAGAT GGTGTCTCGT ATGCGCCCTC CTGTAATTCC ATTTATACCC 
CTTATTCTGA AAGATCTCAC TTTTTTACAC GAGGGCAGCA AAACCTTCAT TGACGGCCTT 
GTGAATGTAG AAAAAATGCA CGCCATTGCT GAAAAAGTGA GGACTGTGCG AAAATACCGG 
AGTAACCAGC TACGGCTGGA TACCGATGTC TCCCCGACTC ATCTGCAAAA TAAAGCCTAC 
GTGCGCCAGT TCCAAGTTAT CGACAACCAG AACCTGCTGT TTGAGTTGTC CTGCAAACTG 
GAGCCCAATA CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031749846.1
CDS	628..2505
Translation	MKPLEKFLKKQSSHLSGPRHLHRRQSVSKILPSFLRDTPGDSSGGERCEEDPSGGPPPSQDCLELPDGLRSPLSFSSDELSPSEPLTPPPGSGGWTLSPPGPLLAPDTPEALLGRVLEQLLSAPRLNEAAELALDDFILTHTLFMPTEELLLQLQQYFCGCSRYTTPAWEGSDPLQRKQAVLSSLLHLLDIYKDALQEEERTFQLIKDFYVLVMRDAPNFPQLEGSVIRLHRLVESAELRLTDGSATQCSKQVKPLFRHFRRIDSCLQPRVAFRGSDEIFCRVYMPDHSYVTIRSRLSASVSDILTSVSEKLQYSEEQGQREEPLILVAVTSAGDKVLLKPDDGCIFTTLGINSHLFACNREELRSLVPLPEEVQLPPEDSHIHRIEAEDLANQLSAFHWELFSCVHELEFIDYVFHVERGRRETANLELLLQRCSEVQHWVSTQTLLCEGPARRTQLLKKFIKTAAICKQNQDLLSFFAIVMGLDNGAVSRLRVTWEKLPGKCKNLFRKFENLTDPCRNHKSYREMVSRMRPPVIPFIPLILKDLTFLHEGSKTFIDGLVNVEKMHAIAEKVRTVRKYRSNQLRLDTDVSPTHLQNKAYVRQFQVIDNQNLLFELSCKLEPNTQ

Target ORF information:

RefSeq Version	XM_031893986.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor like 1 (rapgefl1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031893986.1

ORF Insert Sequence:

ATGAAACCTC TGGAAAAATT CTTGAAAAAG CAAAGCTCTC ATCTTTCTGG ACCCCGACAC 
CTACACAGAA GGCAGAGCGT CTCCAAAATC CTGCCTTCTT TCCTGAGGGA TACTCCGGGG 
GACTCCTCTG GGGGAGAAAG GTGTGAGGAA GATCCATCAG GTGGGCCGCC GCCATCCCAG 
GACTGCCTGG AACTACCAGA TGGACTTCGG TCCCCTCTTT CTTTTTCTTC AGATGAACTA 
TCCCCCTCAG AACCCCTCAC ACCTCCGCCA GGATCTGGGG GCTGGACCCT TTCTCCTCCT 
GGCCCTCTGC TTGCCCCTGA TACCCCTGAG GCTCTACTCG GGAGGGTCCT GGAGCAGCTA 
CTGAGTGCGC CGAGGCTAAA TGAAGCAGCA GAATTGGCCC TGGACGACTT TATCCTCACG 
CATACGCTAT TTATGCCGAC AGAGGAGCTC CTCCTCCAAT TACAGCAGTA CTTCTGCGGC 
TGCAGCCGAT ATACTACTCC GGCGTGGGAA GGGAGCGACC CTCTTCAGAG GAAGCAGGCT 
GTACTCTCAT CTCTTTTACA CCTCTTGGAT ATTTACAAGG ACGCTCTCCA GGAGGAGGAG 
AGGACATTCC AGCTAATCAA GGATTTCTAT GTCTTGGTGA TGAGGGATGC CCCGAATTTC 
CCACAGCTGG AGGGGAGCGT CATCCGATTG CACAGGCTTG TGGAAAGCGC TGAGCTCAGG 
CTTACAGATG GCAGTGCCAC TCAATGCTCA AAGCAAGTCA AACCTCTCTT CCGCCATTTC 
CGAAGAATCG ACTCCTGCCT GCAGCCACGG GTGGCATTCA GGGGCTCTGA CGAGATTTTT 
TGCAGGGTCT ACATGCCTGA TCATTCGTAC GTTACAATTA GGAGCCGACT TTCTGCTTCT 
GTCTCTGATA TCCTGACCTC TGTTTCTGAG AAACTGCAAT ATTCAGAAGA ACAGGGGCAG 
AGAGAGGAAC CCCTGATCCT GGTGGCTGTG ACAAGTGCTG GAGACAAGGT TCTGCTGAAG 
CCAGATGATG GCTGTATCTT TACCACTCTG GGGATTAATA GTCACTTGTT CGCCTGCAAT 
CGGGAGGAGC TGCGCTCTTT GGTTCCCCTT CCAGAGGAAG TTCAACTGCC TCCCGAAGAC 
TCCCATATTC ACAGGATTGA GGCAGAAGAC TTGGCCAATC AACTGAGTGC TTTCCACTGG 
GAACTTTTCA GCTGTGTGCA TGAGCTGGAA TTTATAGACT ATGTGTTCCA CGTGGAGCGA 
GGACGCAGGG AGACGGCGAA TCTGGAACTT TTGTTGCAGA GGTGCAGTGA GGTGCAGCAC 
TGGGTCAGTA CTCAGACTCT GCTCTGCGAG GGGCCAGCAC GCCGCACACA ACTTCTCAAG 
AAATTCATCA AGACAGCTGC TATCTGTAAA CAAAACCAGG ACCTGCTGTC ATTCTTTGCA 
ATCGTGATGG GCTTGGATAA TGGAGCTGTG AGCCGCCTTC GGGTGACATG GGAAAAATTG 
CCAGGAAAAT GTAAGAACTT GTTCCGAAAG TTTGAGAATT TGACGGATCC GTGCCGGAAC 
CACAAATCCT ACCGTGAGAT GGTGTCTCGT ATGCGCCCTC CTGTAATTCC ATTTATACCC 
CTTATTCTGA AAGATCTCAC TTTTTTACAC GAGGGCAGCA AAACCTTCAT TGACGGCCTT 
GTGAATGTAG AAAAAATGCA CGCCATTGCT GAAAAAGTGA GGACTGTGCG AAAATACCGG 
AGTAACCAGC TACGGCTGGA TACCGATGTC TCCCCGACTC ATCTGCAAAA TAAAGCCTAC 
GTGCGCCAGT TCCAAGTTAT CGACAACCAG AACCTGCTGT TTGAGTTGTC CTGCAAACTG 
GAGCCCAATA CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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rapgefl1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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