LOC100694742 cDNA ORF clone, Oreochromis niloticus(Nile tilapia)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100694742
Entrez Gene ID	100694742
Full Name	probable E3 ubiquitin-protein ligase HERC4
Gene Type	protein-coding
Organism	Oreochromis niloticus(Nile tilapia)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019360785.2	XP_019216330.1	probable E3 ubiquitin-protein ligase HERC4
XM_019360785.1	XP_019216330.1	probable E3 ubiquitin-protein ligase HERC4

Pathways from BioSystems
Gene Ontology

The following LOC100694742 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100694742 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOr66900	XM_019360785.2 Latest version!	Oreochromis niloticus probable E3 ubiquitin-protein ligase HERC4 (LOC100694742), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
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OOr66900	XM_019360785.1	Oreochromis niloticus probable E3 ubiquitin-protein ligase HERC4 (LOC100694742), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100694742 ( XM_019360785.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019216330.1 Oreochromis niloticus probable E3 ubiquitin-protein ligase HERC4 (LOC100694742), mRNA.

Price & Availability↑

CloneID	OOr66900
Clone ID Related Accession (Same CDS sequence)	XM_019360785.2 , XM_019360785.1
Accession Version	XM_019360785.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	probable E3 ubiquitin-protein ligase HERC4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031972.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_019360785.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCCT GGGGAGAGGA CTGTCAGCGC GGGTTTTACG TGAAAGACGG CTCCGGTACC 
GACAGCACGA CCACGCATGA TGGAGTTCAT TATTTAAACA TCAGCCATCA CATCGCGGAT 
CTGTCGGCAG GTCGAAACGT ACTGGCTTTC GTTAAAAGTA ACGGAAACGC GTTCATTATC 
CGCACAAACG AGAGCAAGGA TGGCAGAAGA GCGAGAGGCA GGCAGAAGTT TGTGAAACAC 
AAAGAGAAGA TAGAGGCTGT GAGTTGTGGC GATGATGTGG TCGCACTGCT GTCTGAGAGC 
GGCAAAGTCC TCTGTGTGGA CACGAGACAC CCACCCTTCA CTCCCAGCCC ACTGGAAGCT 
TTTTCTAACA AACAGGTTTC TCAGGTTGCT TGTGGGAGTC AGCACTCAGT TGCGCTAACC 
AAAGATGGTC AGCTGTATAC GTGGGGTCAG GACTGCAGGG GCCAGCTGGG TCTGGGTACG 
AGAGAGTCTG TCTGCAGATC ACCTCAGCAT GTCCCATCAC TGTCAGCGAT CCCCCTGATC 
CAGGTCGCTG CAGGAGGAGA CCAGAGTTTC GCCCTCTCTG TCTCTGGAGG TGTTTTCAGC 
TGGGGGAGAA ATGACTGTGG ACAGCTCGGG CTGGGAGACA CAAAAGACAG ACACACACCA 
GCTCCTGTTC AGTGTTTGAA TATGAAGAAA GCTCAGCGCA TTTCCTGTGG ACAGGACCAC 
ACGGCCATTT TGACAAAGCA TGGTGCAGTG TTTACATTTG GCTCCGGTCA ACACGGACAG 
CTCGGCCACA ACTCACTACG CAACGAACTG CGTCCTCGAC TTGTTGCAGA GCTCTGGGGG 
GCAAAGGTCA CCAAGATTAC ATGTGGACGG AATCACACTT TAGCGCTGAC CGAATCCAAG 
CAGGTCTACT CCTTTGGTTG TGAGAATCAA GGGCAGCTGG GACATCGAGA GGAGAGTAAT 
CCATCTGTGC CGCTGCCTGT TCGACTGCCA CAGGGCACCA ATGGGCGCAA AATCAGAAAC 
ATCTTCGCAG GAGAAAACTG TTCATTTGCA ACATGTTCAT CTGATGAGGG CATTCACGAG 
GGTTCAAACA CAGACTGTGG CATTGCATCA CAACACTGTC TTGATAACAT GGTTGACAAA 
TGGATCACTG AATGTGATTT AAAGTCATGG AAAAAGATTA AACAGGAAAT CTTGGAGGCA 
TTTTCCTCTG CATCCTGTCT GAATAAAAGT TTCCTTGAAC TAAGCGGTGA TAAACATTTC 
CAAACTTCAC CAAAGTACCC TGGCTTGAAC ATGAAACATG CACGGCGTGC TTTCACAAAA 
CTGGCAAAGA AGGACAATGT TCTTGCAGAG ATCGAGGCTG CAGTGCTACA CCTGCTTCCT 
TCCCTCGATC AGAAACCAGT GGGAGTGGAG GGGTTGAGAA TCTACCTTCT TCTCAATGAG 
CTTCTGCACA CAGTCCTGAA ACACACACGG CAACAGAGAA TAAAGCTCGC TGTGGCGGTA 
GCCAATGCAG TAACAAGGTT GTCGAATGAA AGCCTCCAGA TTATAGGCGA CTGGTGGTCC 
TCTCTGTCGC ACTCCACCAT GATTCGACAC ATTAACGTGT GGAAGCAGGC CCTCTCAGAG 
ATCCTGTCCT TTGTGCCTGT TCCCCGCAAC TCCGGGGTCA GAAATCTGCT GCTAGTCCTC 
AAATACATGT ACAACGCCAA CAGCAGGGTT GTGGAATCTC GAAGAATACC AGAGAGCAGT 
TTTTATTTAT TGTTGGATGA AGCATTTCTT GGTGAGGATC TGGACCACTG GCATTTACGT 
TCACAAAACG GGTATGTTAA AGCTGAGCCA CTTCTCCTTT GTGACTTCCC AATTGTAATG 
GACCTGCAAT CAAAAAAATG GGTTTTTGAC TCTAATACTG TATACACGCA GATGTTAATG 
CAGAGGAGTT ACTACTTGGA GAATTTCTTT GACTATTGGG GAATTTTTGA TGATTATGAT 
GAAGGTGTGT TTTTACTGGA CCTGAGGCGA GCAACCATTT TGGAAGACAC CTTTGAACAA 
CTGGCGGATG CTTGTGACAC AGACTACAAG AAGCCACTTA GGGTCTTCTT TGATGAAAAT 
CCTATGATCG ATGACGTCTA CAGGAAAGAC TTTTTTTATG AAGTCTTTCA TGACTTGATC 
TCAGCTGAAT CTGGGATGTT CATGTTCAAT GACTCTGAAA CACTGGCATG GTTCTCCTCA 
AAAGTACCTC AGGAAGACCA GCGTTTCTTC CTGTTTGGAG TTCTGTGTGG ATTGGCCTTG 
TACAACCAGT GCATCGTCCA CTTACCCTTC CCACTGGTGC TGTTCAAGAA GCTGCTTGGT 
GTAAGGCCTT CACTGGAGGA TTTGATAGAA TTCAACCCAA GTGTTGGAGA GAGTCTGCAG 
TACATCCTGG AGGACTACAA AGATGATGAC TTGGAAAACC TGGACATGTA TTTTTCGATC 
AACTGGGATG GAAAGGAGAT TGACCTTGAT CCTGAAAGTC CTGAAAAGTT GGTGACAAGT 
CAAAATAAGA AGGAGTTTGT GGATGCCTAT GTGAATCATG CCTTCAACAC ATCAGTGGAG 
AATGTGTTTC AGGAGTTCAA GAGAGGCTTC TTCCTGGTGT GTGAGCGCGA TTTGGTGAAA 
CTCTTCAGGC CAAAGGAGCT GCAGGAAGTG ATGGTGGGCA AAGCCTTCAG TGACTGGGAA 
AAGCTGAAAC AGAACACACA TTATGAAGGT GAATACAGCG CAGACCATCC CACCATACAG 
ATGTTTTGGG AGGTTTTTGA TGAACTGACT GAGAACCAGA AGAAAGCTTT CCTTTGGTTT 
GTGACAGGGT TTGACCGGGT GCCAATTCTC GGTATGGACA AAATCAAGAT GCAAGTCAAA 
GTTAAAGAAG TCAAGGACCT CTCCTATGAC CAGTACTATC CTGAGACACA CACATGCTTC 
TCGACCTTGG AGCTGCCGTT ATACTCAACT AAGGAGATCA TGCAAACCAA ACTCACAGAG 
GCCCTGAGCA ACAATAAGAG GATCCATAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019216330.1
CDS	74..3106
Translation	MFSWGEDCQRGFYVKDGSGTDSTTTHDGVHYLNISHHIADLSAGRNVLAFVKSNGNAFIIRTNESKDGRRARGRQKFVKHKEKIEAVSCGDDVVALLSESGKVLCVDTRHPPFTPSPLEAFSNKQVSQVACGSQHSVALTKDGQLYTWGQDCRGQLGLGTRESVCRSPQHVPSLSAIPLIQVAAGGDQSFALSVSGGVFSWGRNDCGQLGLGDTKDRHTPAPVQCLNMKKAQRISCGQDHTAILTKHGAVFTFGSGQHGQLGHNSLRNELRPRLVAELWGAKVTKITCGRNHTLALTESKQVYSFGCENQGQLGHREESNPSVPLPVRLPQGTNGRKIRNIFAGENCSFATCSSDEGIHEGSNTDCGIASQHCLDNMVDKWITECDLKSWKKIKQEILEAFSSASCLNKSFLELSGDKHFQTSPKYPGLNMKHARRAFTKLAKKDNVLAEIEAAVLHLLPSLDQKPVGVEGLRIYLLLNELLHTVLKHTRQQRIKLAVAVANAVTRLSNESLQIIGDWWSSLSHSTMIRHINVWKQALSEILSFVPVPRNSGVRNLLLVLKYMYNANSRVVESRRIPESSFYLLLDEAFLGEDLDHWHLRSQNGYVKAEPLLLCDFPIVMDLQSKKWVFDSNTVYTQMLMQRSYYLENFFDYWGIFDDYDEGVFLLDLRRATILEDTFEQLADACDTDYKKPLRVFFDENPMIDDVYRKDFFYEVFHDLISAESGMFMFNDSETLAWFSSKVPQEDQRFFLFGVLCGLALYNQCIVHLPFPLVLFKKLLGVRPSLEDLIEFNPSVGESLQYILEDYKDDDLENLDMYFSINWDGKEIDLDPESPEKLVTSQNKKEFVDAYVNHAFNTSVENVFQEFKRGFFLVCERDLVKLFRPKELQEVMVGKAFSDWEKLKQNTHYEGEYSADHPTIQMFWEVFDELTENQKKAFLWFVTGFDRVPILGMDKIKMQVKVKEVKDLSYDQYYPETHTCFSTLELPLYSTKEIMQTKLTEALSNNKRIHK

Target ORF information:

RefSeq Version	XM_019360785.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus probable E3 ubiquitin-protein ligase HERC4 (LOC100694742), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019360785.2

ORF Insert Sequence:

ATGTTTTCCT GGGGAGAGGA CTGTCAGCGC GGGTTTTACG TGAAAGACGG CTCCGGTACC 
GACAGCACGA CCACGCATGA TGGAGTTCAT TATTTAAACA TCAGCCATCA CATCGCGGAT 
CTGTCGGCAG GTCGAAACGT ACTGGCTTTC GTTAAAAGTA ACGGAAACGC GTTCATTATC 
CGCACAAACG AGAGCAAGGA TGGCAGAAGA GCGAGAGGCA GGCAGAAGTT TGTGAAACAC 
AAAGAGAAGA TAGAGGCTGT GAGTTGTGGC GATGATGTGG TCGCACTGCT GTCTGAGAGC 
GGCAAAGTCC TCTGTGTGGA CACGAGACAC CCACCCTTCA CTCCCAGCCC ACTGGAAGCT 
TTTTCTAACA AACAGGTTTC TCAGGTTGCT TGTGGGAGTC AGCACTCAGT TGCGCTAACC 
AAAGATGGTC AGCTGTATAC GTGGGGTCAG GACTGCAGGG GCCAGCTGGG TCTGGGTACG 
AGAGAGTCTG TCTGCAGATC ACCTCAGCAT GTCCCATCAC TGTCAGCGAT CCCCCTGATC 
CAGGTCGCTG CAGGAGGAGA CCAGAGTTTC GCCCTCTCTG TCTCTGGAGG TGTTTTCAGC 
TGGGGGAGAA ATGACTGTGG ACAGCTCGGG CTGGGAGACA CAAAAGACAG ACACACACCA 
GCTCCTGTTC AGTGTTTGAA TATGAAGAAA GCTCAGCGCA TTTCCTGTGG ACAGGACCAC 
ACGGCCATTT TGACAAAGCA TGGTGCAGTG TTTACATTTG GCTCCGGTCA ACACGGACAG 
CTCGGCCACA ACTCACTACG CAACGAACTG CGTCCTCGAC TTGTTGCAGA GCTCTGGGGG 
GCAAAGGTCA CCAAGATTAC ATGTGGACGG AATCACACTT TAGCGCTGAC CGAATCCAAG 
CAGGTCTACT CCTTTGGTTG TGAGAATCAA GGGCAGCTGG GACATCGAGA GGAGAGTAAT 
CCATCTGTGC CGCTGCCTGT TCGACTGCCA CAGGGCACCA ATGGGCGCAA AATCAGAAAC 
ATCTTCGCAG GAGAAAACTG TTCATTTGCA ACATGTTCAT CTGATGAGGG CATTCACGAG 
GGTTCAAACA CAGACTGTGG CATTGCATCA CAACACTGTC TTGATAACAT GGTTGACAAA 
TGGATCACTG AATGTGATTT AAAGTCATGG AAAAAGATTA AACAGGAAAT CTTGGAGGCA 
TTTTCCTCTG CATCCTGTCT GAATAAAAGT TTCCTTGAAC TAAGCGGTGA TAAACATTTC 
CAAACTTCAC CAAAGTACCC TGGCTTGAAC ATGAAACATG CACGGCGTGC TTTCACAAAA 
CTGGCAAAGA AGGACAATGT TCTTGCAGAG ATCGAGGCTG CAGTGCTACA CCTGCTTCCT 
TCCCTCGATC AGAAACCAGT GGGAGTGGAG GGGTTGAGAA TCTACCTTCT TCTCAATGAG 
CTTCTGCACA CAGTCCTGAA ACACACACGG CAACAGAGAA TAAAGCTCGC TGTGGCGGTA 
GCCAATGCAG TAACAAGGTT GTCGAATGAA AGCCTCCAGA TTATAGGCGA CTGGTGGTCC 
TCTCTGTCGC ACTCCACCAT GATTCGACAC ATTAACGTGT GGAAGCAGGC CCTCTCAGAG 
ATCCTGTCCT TTGTGCCTGT TCCCCGCAAC TCCGGGGTCA GAAATCTGCT GCTAGTCCTC 
AAATACATGT ACAACGCCAA CAGCAGGGTT GTGGAATCTC GAAGAATACC AGAGAGCAGT 
TTTTATTTAT TGTTGGATGA AGCATTTCTT GGTGAGGATC TGGACCACTG GCATTTACGT 
TCACAAAACG GGTATGTTAA AGCTGAGCCA CTTCTCCTTT GTGACTTCCC AATTGTAATG 
GACCTGCAAT CAAAAAAATG GGTTTTTGAC TCTAATACTG TATACACGCA GATGTTAATG 
CAGAGGAGTT ACTACTTGGA GAATTTCTTT GACTATTGGG GAATTTTTGA TGATTATGAT 
GAAGGTGTGT TTTTACTGGA CCTGAGGCGA GCAACCATTT TGGAAGACAC CTTTGAACAA 
CTGGCGGATG CTTGTGACAC AGACTACAAG AAGCCACTTA GGGTCTTCTT TGATGAAAAT 
CCTATGATCG ATGACGTCTA CAGGAAAGAC TTTTTTTATG AAGTCTTTCA TGACTTGATC 
TCAGCTGAAT CTGGGATGTT CATGTTCAAT GACTCTGAAA CACTGGCATG GTTCTCCTCA 
AAAGTACCTC AGGAAGACCA GCGTTTCTTC CTGTTTGGAG TTCTGTGTGG ATTGGCCTTG 
TACAACCAGT GCATCGTCCA CTTACCCTTC CCACTGGTGC TGTTCAAGAA GCTGCTTGGT 
GTAAGGCCTT CACTGGAGGA TTTGATAGAA TTCAACCCAA GTGTTGGAGA GAGTCTGCAG 
TACATCCTGG AGGACTACAA AGATGATGAC TTGGAAAACC TGGACATGTA TTTTTCGATC 
AACTGGGATG GAAAGGAGAT TGACCTTGAT CCTGAAAGTC CTGAAAAGTT GGTGACAAGT 
CAAAATAAGA AGGAGTTTGT GGATGCCTAT GTGAATCATG CCTTCAACAC ATCAGTGGAG 
AATGTGTTTC AGGAGTTCAA GAGAGGCTTC TTCCTGGTGT GTGAGCGCGA TTTGGTGAAA 
CTCTTCAGGC CAAAGGAGCT GCAGGAAGTG ATGGTGGGCA AAGCCTTCAG TGACTGGGAA 
AAGCTGAAAC AGAACACACA TTATGAAGGT GAATACAGCG CAGACCATCC CACCATACAG 
ATGTTTTGGG AGGTTTTTGA TGAACTGACT GAGAACCAGA AGAAAGCTTT CCTTTGGTTT 
GTGACAGGGT TTGACCGGGT GCCAATTCTC GGTATGGACA AAATCAAGAT GCAAGTCAAA 
GTTAAAGAAG TCAAGGACCT CTCCTATGAC CAGTACTATC CTGAGACACA CACATGCTTC 
TCGACCTTGG AGCTGCCGTT ATACTCAACT AAGGAGATCA TGCAAACCAA ACTCACAGAG 
GCCCTGAGCA ACAATAAGAG GATCCATAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100694742 ( XM_019360785.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019216330.1 Oreochromis niloticus probable E3 ubiquitin-protein ligase HERC4 (LOC100694742), mRNA.

Price & Availability↑

CloneID	OOr66900
Clone ID Related Accession (Same CDS sequence)	XM_019360785.2 , XM_019360785.1
Accession Version	XM_019360785.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	probable E3 ubiquitin-protein ligase HERC4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031972.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCCT GGGGAGAGGA CTGTCAGCGC GGGTTTTACG TGAAAGACGG CTCCGGTACC 
GACAGCACGA CCACGCATGA TGGAGTTCAT TATTTAAACA TCAGCCATCA CATCGCGGAT 
CTGTCGGCAG GTCGAAACGT ACTGGCTTTC GTTAAAAGTA ACGGAAACGC GTTCATTATC 
CGCACAAACG AGAGCAAGGA TGGCAGAAGA GCGAGAGGCA GGCAGAAGTT TGTGAAACAC 
AAAGAGAAGA TAGAGGCTGT GAGTTGTGGC GATGATGTGG TCGCACTGCT GTCTGAGAGC 
GGCAAAGTCC TCTGTGTGGA CACGAGACAC CCACCCTTCA CTCCCAGCCC ACTGGAAGCT 
TTTTCTAACA AACAGGTTTC TCAGGTTGCT TGTGGGAGTC AGCACTCAGT TGCGCTAACC 
AAAGATGGTC AGCTGTATAC GTGGGGTCAG GACTGCAGGG GCCAGCTGGG TCTGGGTACG 
AGAGAGTCTG TCTGCAGATC ACCTCAGCAT GTCCCATCAC TGTCAGCGAT CCCCCTGATC 
CAGGTCGCTG CAGGAGGAGA CCAGAGTTTC GCCCTCTCTG TCTCTGGAGG TGTTTTCAGC 
TGGGGGAGAA ATGACTGTGG ACAGCTCGGG CTGGGAGACA CAAAAGACAG ACACACACCA 
GCTCCTGTTC AGTGTTTGAA TATGAAGAAA GCTCAGCGCA TTTCCTGTGG ACAGGACCAC 
ACGGCCATTT TGACAAAGCA TGGTGCAGTG TTTACATTTG GCTCCGGTCA ACACGGACAG 
CTCGGCCACA ACTCACTACG CAACGAACTG CGTCCTCGAC TTGTTGCAGA GCTCTGGGGG 
GCAAAGGTCA CCAAGATTAC ATGTGGACGG AATCACACTT TAGCGCTGAC CGAATCCAAG 
CAGGTCTACT CCTTTGGTTG TGAGAATCAA GGGCAGCTGG GACATCGAGA GGAGAGTAAT 
CCATCTGTGC CGCTGCCTGT TCGACTGCCA CAGGGCACCA ATGGGCGCAA AATCAGAAAC 
ATCTTCGCAG GAGAAAACTG TTCATTTGCA ACATGTTCAT CTGATGAGGG CATTCACGAG 
GGTTCAAACA CAGACTGTGG CATTGCATCA CAACACTGTC TTGATAACAT GGTTGACAAA 
TGGATCACTG AATGTGATTT AAAGTCATGG AAAAAGATTA AACAGGAAAT CTTGGAGGCA 
TTTTCCTCTG CATCCTGTCT GAATAAAAGT TTCCTTGAAC TAAGCGGTGA TAAACATTTC 
CAAACTTCAC CAAAGTACCC TGGCTTGAAC ATGAAACATG CACGGCGTGC TTTCACAAAA 
CTGGCAAAGA AGGACAATGT TCTTGCAGAG ATCGAGGCTG CAGTGCTACA CCTGCTTCCT 
TCCCTCGATC AGAAACCAGT GGGAGTGGAG GGGTTGAGAA TCTACCTTCT TCTCAATGAG 
CTTCTGCACA CAGTCCTGAA ACACACACGG CAACAGAGAA TAAAGCTCGC TGTGGCGGTA 
GCCAATGCAG TAACAAGGTT GTCGAATGAA AGCCTCCAGA TTATAGGCGA CTGGTGGTCC 
TCTCTGTCGC ACTCCACCAT GATTCGACAC ATTAACGTGT GGAAGCAGGC CCTCTCAGAG 
ATCCTGTCCT TTGTGCCTGT TCCCCGCAAC TCCGGGGTCA GAAATCTGCT GCTAGTCCTC 
AAATACATGT ACAACGCCAA CAGCAGGGTT GTGGAATCTC GAAGAATACC AGAGAGCAGT 
TTTTATTTAT TGTTGGATGA AGCATTTCTT GGTGAGGATC TGGACCACTG GCATTTACGT 
TCACAAAACG GGTATGTTAA AGCTGAGCCA CTTCTCCTTT GTGACTTCCC AATTGTAATG 
GACCTGCAAT CAAAAAAATG GGTTTTTGAC TCTAATACTG TATACACGCA GATGTTAATG 
CAGAGGAGTT ACTACTTGGA GAATTTCTTT GACTATTGGG GAATTTTTGA TGATTATGAT 
GAAGGTGTGT TTTTACTGGA CCTGAGGCGA GCAACCATTT TGGAAGACAC CTTTGAACAA 
CTGGCGGATG CTTGTGACAC AGACTACAAG AAGCCACTTA GGGTCTTCTT TGATGAAAAT 
CCTATGATCG ATGACGTCTA CAGGAAAGAC TTTTTTTATG AAGTCTTTCA TGACTTGATC 
TCAGCTGAAT CTGGGATGTT CATGTTCAAT GACTCTGAAA CACTGGCATG GTTCTCCTCA 
AAAGTACCTC AGGAAGACCA GCGTTTCTTC CTGTTTGGAG TTCTGTGTGG ATTGGCCTTG 
TACAACCAGT GCATCGTCCA CTTACCCTTC CCACTGGTGC TGTTCAAGAA GCTGCTTGGT 
GTAAGGCCTT CACTGGAGGA TTTGATAGAA TTCAACCCAA GTGTTGGAGA GAGTCTGCAG 
TACATCCTGG AGGACTACAA AGATGATGAC TTGGAAAACC TGGACATGTA TTTTTCGATC 
AACTGGGATG GAAAGGAGAT TGACCTTGAT CCTGAAAGTC CTGAAAAGTT GGTGACAAGT 
CAAAATAAGA AGGAGTTTGT GGATGCCTAT GTGAATCATG CCTTCAACAC ATCAGTGGAG 
AATGTGTTTC AGGAGTTCAA GAGAGGCTTC TTCCTGGTGT GTGAGCGCGA TTTGGTGAAA 
CTCTTCAGGC CAAAGGAGCT GCAGGAAGTG ATGGTGGGCA AAGCCTTCAG TGACTGGGAA 
AAGCTGAAAC AGAACACACA TTATGAAGGT GAATACAGCG CAGACCATCC CACCATACAG 
ATGTTTTGGG AGGTTTTTGA TGAACTGACT GAGAACCAGA AGAAAGCTTT CCTTTGGTTT 
GTGACAGGGT TTGACCGGGT GCCAATTCTC GGTATGGACA AAATCAAGAT GCAAGTCAAA 
GTTAAAGAAG TCAAGGACCT CTCCTATGAC CAGTACTATC CTGAGACACA CACATGCTTC 
TCGACCTTGG AGCTGCCGTT ATACTCAACT AAGGAGATCA TGCAAACCAA ACTCACAGAG 
GCCCTGAGCA ACAATAAGAG GATCCATAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019216330.1
CDS	84..3116
Translation	MFSWGEDCQRGFYVKDGSGTDSTTTHDGVHYLNISHHIADLSAGRNVLAFVKSNGNAFIIRTNESKDGRRARGRQKFVKHKEKIEAVSCGDDVVALLSESGKVLCVDTRHPPFTPSPLEAFSNKQVSQVACGSQHSVALTKDGQLYTWGQDCRGQLGLGTRESVCRSPQHVPSLSAIPLIQVAAGGDQSFALSVSGGVFSWGRNDCGQLGLGDTKDRHTPAPVQCLNMKKAQRISCGQDHTAILTKHGAVFTFGSGQHGQLGHNSLRNELRPRLVAELWGAKVTKITCGRNHTLALTESKQVYSFGCENQGQLGHREESNPSVPLPVRLPQGTNGRKIRNIFAGENCSFATCSSDEGIHEGSNTDCGIASQHCLDNMVDKWITECDLKSWKKIKQEILEAFSSASCLNKSFLELSGDKHFQTSPKYPGLNMKHARRAFTKLAKKDNVLAEIEAAVLHLLPSLDQKPVGVEGLRIYLLLNELLHTVLKHTRQQRIKLAVAVANAVTRLSNESLQIIGDWWSSLSHSTMIRHINVWKQALSEILSFVPVPRNSGVRNLLLVLKYMYNANSRVVESRRIPESSFYLLLDEAFLGEDLDHWHLRSQNGYVKAEPLLLCDFPIVMDLQSKKWVFDSNTVYTQMLMQRSYYLENFFDYWGIFDDYDEGVFLLDLRRATILEDTFEQLADACDTDYKKPLRVFFDENPMIDDVYRKDFFYEVFHDLISAESGMFMFNDSETLAWFSSKVPQEDQRFFLFGVLCGLALYNQCIVHLPFPLVLFKKLLGVRPSLEDLIEFNPSVGESLQYILEDYKDDDLENLDMYFSINWDGKEIDLDPESPEKLVTSQNKKEFVDAYVNHAFNTSVENVFQEFKRGFFLVCERDLVKLFRPKELQEVMVGKAFSDWEKLKQNTHYEGEYSADHPTIQMFWEVFDELTENQKKAFLWFVTGFDRVPILGMDKIKMQVKVKEVKDLSYDQYYPETHTCFSTLELPLYSTKEIMQTKLTEALSNNKRIHK

Target ORF information:

RefSeq Version	XM_019360785.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus probable E3 ubiquitin-protein ligase HERC4 (LOC100694742), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019360785.1

ORF Insert Sequence:

ATGTTTTCCT GGGGAGAGGA CTGTCAGCGC GGGTTTTACG TGAAAGACGG CTCCGGTACC 
GACAGCACGA CCACGCATGA TGGAGTTCAT TATTTAAACA TCAGCCATCA CATCGCGGAT 
CTGTCGGCAG GTCGAAACGT ACTGGCTTTC GTTAAAAGTA ACGGAAACGC GTTCATTATC 
CGCACAAACG AGAGCAAGGA TGGCAGAAGA GCGAGAGGCA GGCAGAAGTT TGTGAAACAC 
AAAGAGAAGA TAGAGGCTGT GAGTTGTGGC GATGATGTGG TCGCACTGCT GTCTGAGAGC 
GGCAAAGTCC TCTGTGTGGA CACGAGACAC CCACCCTTCA CTCCCAGCCC ACTGGAAGCT 
TTTTCTAACA AACAGGTTTC TCAGGTTGCT TGTGGGAGTC AGCACTCAGT TGCGCTAACC 
AAAGATGGTC AGCTGTATAC GTGGGGTCAG GACTGCAGGG GCCAGCTGGG TCTGGGTACG 
AGAGAGTCTG TCTGCAGATC ACCTCAGCAT GTCCCATCAC TGTCAGCGAT CCCCCTGATC 
CAGGTCGCTG CAGGAGGAGA CCAGAGTTTC GCCCTCTCTG TCTCTGGAGG TGTTTTCAGC 
TGGGGGAGAA ATGACTGTGG ACAGCTCGGG CTGGGAGACA CAAAAGACAG ACACACACCA 
GCTCCTGTTC AGTGTTTGAA TATGAAGAAA GCTCAGCGCA TTTCCTGTGG ACAGGACCAC 
ACGGCCATTT TGACAAAGCA TGGTGCAGTG TTTACATTTG GCTCCGGTCA ACACGGACAG 
CTCGGCCACA ACTCACTACG CAACGAACTG CGTCCTCGAC TTGTTGCAGA GCTCTGGGGG 
GCAAAGGTCA CCAAGATTAC ATGTGGACGG AATCACACTT TAGCGCTGAC CGAATCCAAG 
CAGGTCTACT CCTTTGGTTG TGAGAATCAA GGGCAGCTGG GACATCGAGA GGAGAGTAAT 
CCATCTGTGC CGCTGCCTGT TCGACTGCCA CAGGGCACCA ATGGGCGCAA AATCAGAAAC 
ATCTTCGCAG GAGAAAACTG TTCATTTGCA ACATGTTCAT CTGATGAGGG CATTCACGAG 
GGTTCAAACA CAGACTGTGG CATTGCATCA CAACACTGTC TTGATAACAT GGTTGACAAA 
TGGATCACTG AATGTGATTT AAAGTCATGG AAAAAGATTA AACAGGAAAT CTTGGAGGCA 
TTTTCCTCTG CATCCTGTCT GAATAAAAGT TTCCTTGAAC TAAGCGGTGA TAAACATTTC 
CAAACTTCAC CAAAGTACCC TGGCTTGAAC ATGAAACATG CACGGCGTGC TTTCACAAAA 
CTGGCAAAGA AGGACAATGT TCTTGCAGAG ATCGAGGCTG CAGTGCTACA CCTGCTTCCT 
TCCCTCGATC AGAAACCAGT GGGAGTGGAG GGGTTGAGAA TCTACCTTCT TCTCAATGAG 
CTTCTGCACA CAGTCCTGAA ACACACACGG CAACAGAGAA TAAAGCTCGC TGTGGCGGTA 
GCCAATGCAG TAACAAGGTT GTCGAATGAA AGCCTCCAGA TTATAGGCGA CTGGTGGTCC 
TCTCTGTCGC ACTCCACCAT GATTCGACAC ATTAACGTGT GGAAGCAGGC CCTCTCAGAG 
ATCCTGTCCT TTGTGCCTGT TCCCCGCAAC TCCGGGGTCA GAAATCTGCT GCTAGTCCTC 
AAATACATGT ACAACGCCAA CAGCAGGGTT GTGGAATCTC GAAGAATACC AGAGAGCAGT 
TTTTATTTAT TGTTGGATGA AGCATTTCTT GGTGAGGATC TGGACCACTG GCATTTACGT 
TCACAAAACG GGTATGTTAA AGCTGAGCCA CTTCTCCTTT GTGACTTCCC AATTGTAATG 
GACCTGCAAT CAAAAAAATG GGTTTTTGAC TCTAATACTG TATACACGCA GATGTTAATG 
CAGAGGAGTT ACTACTTGGA GAATTTCTTT GACTATTGGG GAATTTTTGA TGATTATGAT 
GAAGGTGTGT TTTTACTGGA CCTGAGGCGA GCAACCATTT TGGAAGACAC CTTTGAACAA 
CTGGCGGATG CTTGTGACAC AGACTACAAG AAGCCACTTA GGGTCTTCTT TGATGAAAAT 
CCTATGATCG ATGACGTCTA CAGGAAAGAC TTTTTTTATG AAGTCTTTCA TGACTTGATC 
TCAGCTGAAT CTGGGATGTT CATGTTCAAT GACTCTGAAA CACTGGCATG GTTCTCCTCA 
AAAGTACCTC AGGAAGACCA GCGTTTCTTC CTGTTTGGAG TTCTGTGTGG ATTGGCCTTG 
TACAACCAGT GCATCGTCCA CTTACCCTTC CCACTGGTGC TGTTCAAGAA GCTGCTTGGT 
GTAAGGCCTT CACTGGAGGA TTTGATAGAA TTCAACCCAA GTGTTGGAGA GAGTCTGCAG 
TACATCCTGG AGGACTACAA AGATGATGAC TTGGAAAACC TGGACATGTA TTTTTCGATC 
AACTGGGATG GAAAGGAGAT TGACCTTGAT CCTGAAAGTC CTGAAAAGTT GGTGACAAGT 
CAAAATAAGA AGGAGTTTGT GGATGCCTAT GTGAATCATG CCTTCAACAC ATCAGTGGAG 
AATGTGTTTC AGGAGTTCAA GAGAGGCTTC TTCCTGGTGT GTGAGCGCGA TTTGGTGAAA 
CTCTTCAGGC CAAAGGAGCT GCAGGAAGTG ATGGTGGGCA AAGCCTTCAG TGACTGGGAA 
AAGCTGAAAC AGAACACACA TTATGAAGGT GAATACAGCG CAGACCATCC CACCATACAG 
ATGTTTTGGG AGGTTTTTGA TGAACTGACT GAGAACCAGA AGAAAGCTTT CCTTTGGTTT 
GTGACAGGGT TTGACCGGGT GCCAATTCTC GGTATGGACA AAATCAAGAT GCAAGTCAAA 
GTTAAAGAAG TCAAGGACCT CTCCTATGAC CAGTACTATC CTGAGACACA CACATGCTTC 
TCGACCTTGG AGCTGCCGTT ATACTCAACT AAGGAGATCA TGCAAACCAA ACTCACAGAG 
GCCCTGAGCA ACAATAAGAG GATCCATAAG TGA