nelfcd cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nelfcd
Entrez Gene ID	111579741
Full Name	negative elongation factor complex member C/D
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023287137.1	XP_023142905.1	negative elongation factor C/D

Pathways from BioSystems
Gene Ontology

The following nelfcd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nelfcd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm91165	XM_023287137.1 Latest version!	Amphiprion ocellaris negative elongation factor complex member C/D (nelfcd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nelfcd ( XM_023287137.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023142905.1 Amphiprion ocellaris negative elongation factor complex member C/D (nelfcd), mRNA.

Price & Availability↑

CloneID	OAm91165
Clone ID Related Accession (Same CDS sequence)	XM_023287137.1
Accession Version	XM_023287137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	negative elongation factor C/D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019373313.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AATATTACAG CGGCAGCGGA GACTGGGAGG GCACGGAGGG AAGCCTGGAG 
GATGGTTATG GTGAAGGAGA AAATGATGGG AAAATCCAAG AAGATTGTCT GCAGAAATTC 
TCCAGTAGGG ATTACATCAT GGAGCCTGCC ATCTTCAACA CCCTCAAAAC GTATTTCCAA 
GCAGGTGGCT CTCCAGAACA TGTCATCCAG CTTCTCTCTG AAAACTACTC TGCTGTGGCT 
CAGACTGTCA ATCTGCTGGC TGAGTGGCTC ATCCAGATGG GTGTAGAGCC AGCTCAGGTC 
CAAGAGCGCG TAGAAAATCA CCTCAAAAGT CTCCTGATTA AGCACTTTGA CCCCCAGAAA 
GCTGATTCCA TCTTCACTGT TGAGGGAGAG ACTCCTGCTT GGCTCGAGCA GATGATAGCA 
CACACAACAT GGAGAGATCT TTTCTATAAG CTGGCAGAGG CTCACCCCGA CTGCCTGATG 
CTCAACTTCA CTGTAAAGCT CATCTCAGAT GCGGGTTACC AGGGCGAAAT CACCAGTGTG 
TCTACAGCAT GTCAGCAGCT GGAGGTTTTC TCTCGGGTGT TGAGGACGTC TTTGGCCACA 
CTGCTGGACG GAGGAGAAGA AAACCTGGAG AAGAACCTGC CAGAGTTTGC TAAGATGGTG 
TGTCACGGCG AGCACACCTA CCTCTTTGCT CAGGCCATGA TGTCCATCCT TGCACAGGAG 
GATCAGGGAG GATCAGCTGT TCGTAGAATT GGTCAGGAGG TGCAAAAGTC TGCGCATGAG 
AGAGGTCATG ATGCCAGTCA GATAACTCTG GCGCTCGGTA CGGCAGCAGC TTATCCTCGA 
GCCTGCCAGG CGCTGGGAGC CATGTTGTCC AAAGGGGCCC TGAATCCTGC TGATATTACA 
GTTCTGTTCA AAATGTTCAG CAGCATGGAC CCTCCTCCTG TGGAGCTGAT CAGAGTGCCT 
GCCTTCCTAG ACCTGTTCAT GCAGTCACTT TTTAAGCCTG GATCAAAGAT CAACCAAGAC 
CATAAACACA AATACATCCA CATCTTGGCC TATGCTGCCA GTGTGGTTGA GACATGGAAA 
AAGAACAAGC GAGTCAACAT CAATAAAGAC GAGCTGAAGT CCACCTCCAA GGCCATAGAG 
ACTGTGCACA ACCTGTGCTG CAATGAGAAC AAAGGAGCTA CAGAGCTGGT GGCTGAACTC 
GGCACTTTGT ACCAGTGCAT CAGGTTTCCA GTAGTTGCTA TGGGAGTTTT GAAGTGGGTG 
GACTGGACAG TGTCTGAGCC GCGGTACTTC CAGCTCCAGA CAGATCACAC TCCAGTCCAC 
TTAGCTTTAC TGGATGAGAT CTGCACGTGC CATCAGCTGC TGCACCCGCA GGTCCTCCAG 
CTGCTCATCA AGCTCTTTGA GACGGAGCAC TCTCAGCTGG ACGTCATGGA GCAGTTGGAG 
CTGAAGAAGA CTCTGCTGGA CAGAATGGTT CATCTGCTGA GTCGCGGCTA CGTCCTGCCT 
GTCGTCGGTT ACATTCGCAA GTGTCTAGAA AAACTCAACA CAGACATCTC CCTCATCAGA 
TATTTTGTCA CTGAGGTGCT GGACGTGATT GCGCCTCCGT ACACGTCAGA CTTTGTTCAG 
CTCTTCTTGC CCATCCTGGA GAACGACAGC ATTGCAGGAA CAATCCGCAC AGAGGGAGAG 
CATGATCCTG TTGCTGAGTT CATTGCTCAT TGCAAGTCAA ACTTCATTAT GATTAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023142905.1
CDS	158..1897
Translation	MDEEYYSGSGDWEGTEGSLEDGYGEGENDGKIQEDCLQKFSSRDYIMEPAIFNTLKTYFQAGGSPEHVIQLLSENYSAVAQTVNLLAEWLIQMGVEPAQVQERVENHLKSLLIKHFDPQKADSIFTVEGETPAWLEQMIAHTTWRDLFYKLAEAHPDCLMLNFTVKLISDAGYQGEITSVSTACQQLEVFSRVLRTSLATLLDGGEENLEKNLPEFAKMVCHGEHTYLFAQAMMSILAQEDQGGSAVRRIGQEVQKSAHERGHDASQITLALGTAAAYPRACQALGAMLSKGALNPADITVLFKMFSSMDPPPVELIRVPAFLDLFMQSLFKPGSKINQDHKHKYIHILAYAASVVETWKKNKRVNINKDELKSTSKAIETVHNLCCNENKGATELVAELGTLYQCIRFPVVAMGVLKWVDWTVSEPRYFQLQTDHTPVHLALLDEICTCHQLLHPQVLQLLIKLFETEHSQLDVMEQLELKKTLLDRMVHLLSRGYVLPVVGYIRKCLEKLNTDISLIRYFVTEVLDVIAPPYTSDFVQLFLPILENDSIAGTIRTEGEHDPVAEFIAHCKSNFIMIN

Target ORF information:

RefSeq Version	XM_023287137.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris negative elongation factor complex member C/D (nelfcd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023287137.1

ORF Insert Sequence:

ATGGACGAGG AATATTACAG CGGCAGCGGA GACTGGGAGG GCACGGAGGG AAGCCTGGAG 
GATGGTTATG GTGAAGGAGA AAATGATGGG AAAATCCAAG AAGATTGTCT GCAGAAATTC 
TCCAGTAGGG ATTACATCAT GGAGCCTGCC ATCTTCAACA CCCTCAAAAC GTATTTCCAA 
GCAGGTGGCT CTCCAGAACA TGTCATCCAG CTTCTCTCTG AAAACTACTC TGCTGTGGCT 
CAGACTGTCA ATCTGCTGGC TGAGTGGCTC ATCCAGATGG GTGTAGAGCC AGCTCAGGTC 
CAAGAGCGCG TAGAAAATCA CCTCAAAAGT CTCCTGATTA AGCACTTTGA CCCCCAGAAA 
GCTGATTCCA TCTTCACTGT TGAGGGAGAG ACTCCTGCTT GGCTCGAGCA GATGATAGCA 
CACACAACAT GGAGAGATCT TTTCTATAAG CTGGCAGAGG CTCACCCCGA CTGCCTGATG 
CTCAACTTCA CTGTAAAGCT CATCTCAGAT GCGGGTTACC AGGGCGAAAT CACCAGTGTG 
TCTACAGCAT GTCAGCAGCT GGAGGTTTTC TCTCGGGTGT TGAGGACGTC TTTGGCCACA 
CTGCTGGACG GAGGAGAAGA AAACCTGGAG AAGAACCTGC CAGAGTTTGC TAAGATGGTG 
TGTCACGGCG AGCACACCTA CCTCTTTGCT CAGGCCATGA TGTCCATCCT TGCACAGGAG 
GATCAGGGAG GATCAGCTGT TCGTAGAATT GGTCAGGAGG TGCAAAAGTC TGCGCATGAG 
AGAGGTCATG ATGCCAGTCA GATAACTCTG GCGCTCGGTA CGGCAGCAGC TTATCCTCGA 
GCCTGCCAGG CGCTGGGAGC CATGTTGTCC AAAGGGGCCC TGAATCCTGC TGATATTACA 
GTTCTGTTCA AAATGTTCAG CAGCATGGAC CCTCCTCCTG TGGAGCTGAT CAGAGTGCCT 
GCCTTCCTAG ACCTGTTCAT GCAGTCACTT TTTAAGCCTG GATCAAAGAT CAACCAAGAC 
CATAAACACA AATACATCCA CATCTTGGCC TATGCTGCCA GTGTGGTTGA GACATGGAAA 
AAGAACAAGC GAGTCAACAT CAATAAAGAC GAGCTGAAGT CCACCTCCAA GGCCATAGAG 
ACTGTGCACA ACCTGTGCTG CAATGAGAAC AAAGGAGCTA CAGAGCTGGT GGCTGAACTC 
GGCACTTTGT ACCAGTGCAT CAGGTTTCCA GTAGTTGCTA TGGGAGTTTT GAAGTGGGTG 
GACTGGACAG TGTCTGAGCC GCGGTACTTC CAGCTCCAGA CAGATCACAC TCCAGTCCAC 
TTAGCTTTAC TGGATGAGAT CTGCACGTGC CATCAGCTGC TGCACCCGCA GGTCCTCCAG 
CTGCTCATCA AGCTCTTTGA GACGGAGCAC TCTCAGCTGG ACGTCATGGA GCAGTTGGAG 
CTGAAGAAGA CTCTGCTGGA CAGAATGGTT CATCTGCTGA GTCGCGGCTA CGTCCTGCCT 
GTCGTCGGTT ACATTCGCAA GTGTCTAGAA AAACTCAACA CAGACATCTC CCTCATCAGA 
TATTTTGTCA CTGAGGTGCT GGACGTGATT GCGCCTCCGT ACACGTCAGA CTTTGTTCAG 
CTCTTCTTGC CCATCCTGGA GAACGACAGC ATTGCAGGAA CAATCCGCAC AGAGGGAGAG 
CATGATCCTG TTGCTGAGTT CATTGCTCAT TGCAAGTCAA ACTTCATTAT GATTAATTGA