daglb cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	daglb
Entrez Gene ID	111579272
Full Name	diacylglycerol lipase beta
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023286494.1	XP_023142262.1	sn1-specific diacylglycerol lipase beta

Pathways from BioSystems
Gene Ontology

The following daglb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the daglb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm90651	XM_023286494.1 Latest version!	Amphiprion ocellaris diacylglycerol lipase beta (daglb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

daglb ( XM_023286494.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023142262.1 Amphiprion ocellaris diacylglycerol lipase beta (daglb), mRNA.

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CloneID	OAm90651
Clone ID Related Accession (Same CDS sequence)	XM_023286494.1
Accession Version	XM_023286494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	sn1-specific diacylglycerol lipase beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019373266.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAA TGGTGGTGTT TGGTCGGCGA TGGGGGATAG CCAGTGATGA TCTTGTGTTC 
CCCGGATCCT TTGAACTGTT CATCCGTGTG CTTTGGTGGA TTGGCACCAT GATCCTGTTC 
ACTTACCACA AGGGTCATTT TGATTGTAAT GAGAGAGGAG TTCTTCACAG CTACCTGGTT 
GGACTGCTGG TTGTGCTAGC GCTCATCATC CTGTCACTGT GTGCTATTGT CTACGTCAGT 
GCACAAGGTA CCATTACAAA TCCTGGTCCA CGGCGCTCTA TGCCTGCACT GGTGTACCTG 
CGAGCTCTGC TGTACCTCCC TGAGCTGGTC TGGGCTTGTC TAGGGGCTGT TTGGGTGTCT 
GATGACAGCA AAGGCTGTGA TCCCGCCACA GTGGGCGCTG TCATTGCAGC AGTTGTTGCC 
AGCTGGATCA TTCTGCTGTT CACGGGGATA GGTGTTGTGT TTGTCTTTGA TCCACTGGGG 
GACCCTCGTC CTCAACCTCC TACCATGGAG CCACTGGGAG TACGAGACCT ACAGAGCAGC 
GAGGGCTCCC AGTTCCTGTC CACTGCTCGC TCCCTGGCCG TTAAAGTCTG GGAGAGCAGG 
CTTCGACTCC TGTGCTGCTG TCTGCCACAG GATGAAAGCC ACCGGGCAGC GTTCTCCAGC 
ATCGCACAGC TCTTTAGCAG CTTCTTCTCT GACACAGACC TGGTTCCCAG TGACATAGCA 
GCCGGTTTGG CTCTGCTGCA CCAGGAGCAA GACAAGATGG AGAAAAGCAG AGATCCAGAA 
GTCATCGTCA GTCACAGCCC TTCCTCTCCA ATCGGAGAAG ATTTGGAGAC AGAGTTGGAG 
AAAGCTGCCC ATTGTATGCA GTTTGCTGCA GCAGCTTATG GTTGGCCACT CTACATTTAC 
TCCAACCCTC TGACTGGACC CTGCAAACTC AGTGGAGACT GCTGTAGGAG CCGGGCAGCT 
GAGTATGAGA TTGTTGGAGG AGACCACCTT GGCTGCCATT TCTCCTCCAT CCTGCAGAGC 
ACTGGTTTGC AGTACAGAGA CTTCATACAT GTCAGCTTCC ACAACCAGAT CTATGAGATC 
CCCTTCTATG TGGCTCTGGA TCATAAGAGG GAGGCTGTTC TGGTGGCTGT CAGAGGAACA 
TTGTCACTGA AGGACGTCCT GACAGACCTG TCAGCTGAAT GTGAGAACCT GCCGATAGAA 
GGAGTGTCTG GAGCCTGCTA CGCTCACAAG GGCATCTGTC AGGCAGCGAG TTACATCTAC 
AAGAAGCTGG TCAACGACGG CATCCTGAAC CAGGCATTCT CCATTGCACC GGAGTATAAA 
CTGGTCATCA CTGGTCACAG TCTGGGCGCT GGCGCAGCTT CAGTGCTGGC TATCCTTTTA 
CGCAATTCCT TCCCCACCCT GCAGTGCTAC GCCTTCTCTC CACCAGGGGG ACTCCTGAGT 
AAAGCCTTAG CTGATTACTC CAAGGACTTT GTGATCTCAG TGGTGCTGGG AAAAGACCTG 
GTTCCCAGAC TGAGCATTCC CAACATGGAG GACCTGAAGA GGAGGATACT TAAAATAGTT 
TCTAACTGCA ATAAGCCCAA ATATCGCATC CTGCTGCAGG GATGCTGGTA CGAGCTGTTT 
GGAGGAGACC CAGATGACTT CCCCACCGAG ATGGACAACA GGAGGGAGGA GGAGCTTGGC 
CAGCCGCTGC TGGGGGAGGA GTCACTGATG ATTCGCCACT CATCATCCTA TCAGAGCCTT 
GCGTCCGATG ACTCGCCTGC CCATACAGCT ACACACTTAC CGCTCTTCCT GCCTGGACGC 
GTTCTGCATA TCACAGAAGA CGGGCCTTCA CGGAGATCCT GTTTTTCTCA GGTCCGTTAC 
CGTGCCGACT GGTCCAATGA AATGGCGTTC AGGAGTATTT TGATCAGCCC CAGGATGCTG 
GCCGATCACA TGCCTGACGC TGTGCTCCGA GCACTCAACA GTCTCACCAG CGACCGGCCC 
TTCTCCCTCT GCCCTTCGTC TTTAAACAAC AGCCAGCACA ATACTATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023142262.1
CDS	645..2675
Translation	MPGMVVFGRRWGIASDDLVFPGSFELFIRVLWWIGTMILFTYHKGHFDCNERGVLHSYLVGLLVVLALIILSLCAIVYVSAQGTITNPGPRRSMPALVYLRALLYLPELVWACLGAVWVSDDSKGCDPATVGAVIAAVVASWIILLFTGIGVVFVFDPLGDPRPQPPTMEPLGVRDLQSSEGSQFLSTARSLAVKVWESRLRLLCCCLPQDESHRAAFSSIAQLFSSFFSDTDLVPSDIAAGLALLHQEQDKMEKSRDPEVIVSHSPSSPIGEDLETELEKAAHCMQFAAAAYGWPLYIYSNPLTGPCKLSGDCCRSRAAEYEIVGGDHLGCHFSSILQSTGLQYRDFIHVSFHNQIYEIPFYVALDHKREAVLVAVRGTLSLKDVLTDLSAECENLPIEGVSGACYAHKGICQAASYIYKKLVNDGILNQAFSIAPEYKLVITGHSLGAGAASVLAILLRNSFPTLQCYAFSPPGGLLSKALADYSKDFVISVVLGKDLVPRLSIPNMEDLKRRILKIVSNCNKPKYRILLQGCWYELFGGDPDDFPTEMDNRREEELGQPLLGEESLMIRHSSSYQSLASDDSPAHTATHLPLFLPGRVLHITEDGPSRRSCFSQVRYRADWSNEMAFRSILISPRMLADHMPDAVLRALNSLTSDRPFSLCPSSLNNSQHNTI

Target ORF information:

RefSeq Version	XM_023286494.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris diacylglycerol lipase beta (daglb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023286494.1

ORF Insert Sequence:

ATGCCTGGAA TGGTGGTGTT TGGTCGGCGA TGGGGGATAG CCAGTGATGA TCTTGTGTTC 
CCCGGATCCT TTGAACTGTT CATCCGTGTG CTTTGGTGGA TTGGCACCAT GATCCTGTTC 
ACTTACCACA AGGGTCATTT TGATTGTAAT GAGAGAGGAG TTCTTCACAG CTACCTGGTT 
GGACTGCTGG TTGTGCTAGC GCTCATCATC CTGTCACTGT GTGCTATTGT CTACGTCAGT 
GCACAAGGTA CCATTACAAA TCCTGGTCCA CGGCGCTCTA TGCCTGCACT GGTGTACCTG 
CGAGCTCTGC TGTACCTCCC TGAGCTGGTC TGGGCTTGTC TAGGGGCTGT TTGGGTGTCT 
GATGACAGCA AAGGCTGTGA TCCCGCCACA GTGGGCGCTG TCATTGCAGC AGTTGTTGCC 
AGCTGGATCA TTCTGCTGTT CACGGGGATA GGTGTTGTGT TTGTCTTTGA TCCACTGGGG 
GACCCTCGTC CTCAACCTCC TACCATGGAG CCACTGGGAG TACGAGACCT ACAGAGCAGC 
GAGGGCTCCC AGTTCCTGTC CACTGCTCGC TCCCTGGCCG TTAAAGTCTG GGAGAGCAGG 
CTTCGACTCC TGTGCTGCTG TCTGCCACAG GATGAAAGCC ACCGGGCAGC GTTCTCCAGC 
ATCGCACAGC TCTTTAGCAG CTTCTTCTCT GACACAGACC TGGTTCCCAG TGACATAGCA 
GCCGGTTTGG CTCTGCTGCA CCAGGAGCAA GACAAGATGG AGAAAAGCAG AGATCCAGAA 
GTCATCGTCA GTCACAGCCC TTCCTCTCCA ATCGGAGAAG ATTTGGAGAC AGAGTTGGAG 
AAAGCTGCCC ATTGTATGCA GTTTGCTGCA GCAGCTTATG GTTGGCCACT CTACATTTAC 
TCCAACCCTC TGACTGGACC CTGCAAACTC AGTGGAGACT GCTGTAGGAG CCGGGCAGCT 
GAGTATGAGA TTGTTGGAGG AGACCACCTT GGCTGCCATT TCTCCTCCAT CCTGCAGAGC 
ACTGGTTTGC AGTACAGAGA CTTCATACAT GTCAGCTTCC ACAACCAGAT CTATGAGATC 
CCCTTCTATG TGGCTCTGGA TCATAAGAGG GAGGCTGTTC TGGTGGCTGT CAGAGGAACA 
TTGTCACTGA AGGACGTCCT GACAGACCTG TCAGCTGAAT GTGAGAACCT GCCGATAGAA 
GGAGTGTCTG GAGCCTGCTA CGCTCACAAG GGCATCTGTC AGGCAGCGAG TTACATCTAC 
AAGAAGCTGG TCAACGACGG CATCCTGAAC CAGGCATTCT CCATTGCACC GGAGTATAAA 
CTGGTCATCA CTGGTCACAG TCTGGGCGCT GGCGCAGCTT CAGTGCTGGC TATCCTTTTA 
CGCAATTCCT TCCCCACCCT GCAGTGCTAC GCCTTCTCTC CACCAGGGGG ACTCCTGAGT 
AAAGCCTTAG CTGATTACTC CAAGGACTTT GTGATCTCAG TGGTGCTGGG AAAAGACCTG 
GTTCCCAGAC TGAGCATTCC CAACATGGAG GACCTGAAGA GGAGGATACT TAAAATAGTT 
TCTAACTGCA ATAAGCCCAA ATATCGCATC CTGCTGCAGG GATGCTGGTA CGAGCTGTTT 
GGAGGAGACC CAGATGACTT CCCCACCGAG ATGGACAACA GGAGGGAGGA GGAGCTTGGC 
CAGCCGCTGC TGGGGGAGGA GTCACTGATG ATTCGCCACT CATCATCCTA TCAGAGCCTT 
GCGTCCGATG ACTCGCCTGC CCATACAGCT ACACACTTAC CGCTCTTCCT GCCTGGACGC 
GTTCTGCATA TCACAGAAGA CGGGCCTTCA CGGAGATCCT GTTTTTCTCA GGTCCGTTAC 
CGTGCCGACT GGTCCAATGA AATGGCGTTC AGGAGTATTT TGATCAGCCC CAGGATGCTG 
GCCGATCACA TGCCTGACGC TGTGCTCCGA GCACTCAACA GTCTCACCAG CGACCGGCCC 
TTCTCCCTCT GCCCTTCGTC TTTAAACAAC AGCCAGCACA ATACTATCTG A