ppcs cDNA ORF clone, Fundulus heteroclitus(mummichog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ppcs
Entrez Gene ID	105933684
Full Name	phosphopantothenoylcysteine synthetase
Gene Type	protein-coding
Organism	Fundulus heteroclitus(mummichog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012873328.1	XP_012728782.1	phosphopantothenate--cysteine ligase
XM_021321912.1	XP_021177587.1	phosphopantothenate--cysteine ligase

Pathways from BioSystems
Gene Ontology

The following ppcs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppcs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFu118186	XM_012873328.1 Latest version!	Fundulus heteroclitus phosphopantothenoylcysteine synthetase (ppcs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OFu140663	XM_021321912.1 Latest version!	Fundulus heteroclitus phosphopantothenoylcysteine synthetase (ppcs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppcs ( XM_012873328.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012728782.1 Fundulus heteroclitus phosphopantothenoylcysteine synthetase (ppcs), mRNA.

Price & Availability↑

CloneID	OFu118186
Clone ID Related Accession (Same CDS sequence)	XM_012873328.1
Accession Version	XM_012873328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	phosphopantothenate--cysteine ligase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012234382.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGC CCCGAACATC GTCCATTGAC GGAAAGCTGG CTGAGGAGTT TGCCGTTCCC 
GCCCACGTGG AGGAGGTGAG GGAGAAGATG GCCGCTTTCG CCTGCCGCCA CGCCGAAGCA 
GGCCGCAGGG TGGTTCTCAT CACCTCAGGA GGCACCAAAG TCCCTCTGGA GTCGCGCACC 
GTCCGCTTCC TGGATAACTT CAGCAGCGGC AGGCGGGGAG CCTCCTCGGC GGAGTACTTT 
ATTGACTCGG GCTACGCCGT CATCTTCCTG CACCGGCACC GCTCTCTCTA CCCGTACACG 
CGCATGTTCT TAACCATCAA CATGCTGGAC GCCCTGCAGT TCAGGGGCGG CGAAGGAGCC 
GGCGAGGTGG TGGTCAACCA GCAGGTGCTC CCGGGCATCG CCGGGGTCCT GAAGAGGTAC 
AACCAGGTGA AAGACGGCGG GCTTCTTCTA CCCATCGAGT TCAACACGCT TTCAGAGTAT 
CTGCATTTAC TGAAGGCAGC GGCGCAGGCG CTCAGCGCTA TAGGATCAAA GGCCATGTTC 
TACTTGGCTG CAGCCGTGTC AGATTTCTAC ATACCGGCGT CAGAGATGCC TGAACACAAA 
ATCCAGTCCT CCAACGGACC TCTTCAGCTC AGCATGAAGA TGGTTCCTAA GCTGCTGTCG 
CCGCTGGTTA AGGACTGGGC GCCTCAGGCG TTCGTCACAT CCTTCAAGCT GGAGACCGAC 
TCGGCCATCC TACTGGACAG GGCCCGGCAG GCTCTGGCCG CTTACAGACA CCAGGCGGTG 
GTGGCCAACG TGCTGGACTC CCGGCGGGGC TACGTGGTGG TGGTGACCCC GGACACCCAG 
GCAGAGCTGG TCCTCACCGA GGACGAGGCG GAGAAGGACG TGGAGATCGA GGAGAGGATA 
GTGAGCAACC TGACGTCGGC GCACAGCAAG TTCATAAGTC AACAAGCCGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012728782.1
CDS	8..961
Translation	MAEPRTSSIDGKLAEEFAVPAHVEEVREKMAAFACRHAEAGRRVVLITSGGTKVPLESRTVRFLDNFSSGRRGASSAEYFIDSGYAVIFLHRHRSLYPYTRMFLTINMLDALQFRGGEGAGEVVVNQQVLPGIAGVLKRYNQVKDGGLLLPIEFNTLSEYLHLLKAAAQALSAIGSKAMFYLAAAVSDFYIPASEMPEHKIQSSNGPLQLSMKMVPKLLSPLVKDWAPQAFVTSFKLETDSAILLDRARQALAAYRHQAVVANVLDSRRGYVVVVTPDTQAELVLTEDEAEKDVEIEERIVSNLTSAHSKFISQQAG

Target ORF information:

RefSeq Version	XM_012873328.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus phosphopantothenoylcysteine synthetase (ppcs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012873328.1

ORF Insert Sequence:

ATGGCTGAGC CCCGAACATC GTCCATTGAC GGAAAGCTGG CTGAGGAGTT TGCCGTTCCC 
GCCCACGTGG AGGAGGTGAG GGAGAAGATG GCCGCTTTCG CCTGCCGCCA CGCCGAAGCA 
GGCCGCAGGG TGGTTCTCAT CACCTCAGGA GGCACCAAAG TCCCTCTGGA GTCGCGCACC 
GTCCGCTTCC TGGATAACTT CAGCAGCGGC AGGCGGGGAG CCTCCTCGGC GGAGTACTTT 
ATTGACTCGG GCTACGCCGT CATCTTCCTG CACCGGCACC GCTCTCTCTA CCCGTACACG 
CGCATGTTCT TAACCATCAA CATGCTGGAC GCCCTGCAGT TCAGGGGCGG CGAAGGAGCC 
GGCGAGGTGG TGGTCAACCA GCAGGTGCTC CCGGGCATCG CCGGGGTCCT GAAGAGGTAC 
AACCAGGTGA AAGACGGCGG GCTTCTTCTA CCCATCGAGT TCAACACGCT TTCAGAGTAT 
CTGCATTTAC TGAAGGCAGC GGCGCAGGCG CTCAGCGCTA TAGGATCAAA GGCCATGTTC 
TACTTGGCTG CAGCCGTGTC AGATTTCTAC ATACCGGCGT CAGAGATGCC TGAACACAAA 
ATCCAGTCCT CCAACGGACC TCTTCAGCTC AGCATGAAGA TGGTTCCTAA GCTGCTGTCG 
CCGCTGGTTA AGGACTGGGC GCCTCAGGCG TTCGTCACAT CCTTCAAGCT GGAGACCGAC 
TCGGCCATCC TACTGGACAG GGCCCGGCAG GCTCTGGCCG CTTACAGACA CCAGGCGGTG 
GTGGCCAACG TGCTGGACTC CCGGCGGGGC TACGTGGTGG TGGTGACCCC GGACACCCAG 
GCAGAGCTGG TCCTCACCGA GGACGAGGCG GAGAAGGACG TGGAGATCGA GGAGAGGATA 
GTGAGCAACC TGACGTCGGC GCACAGCAAG TTCATAAGTC AACAAGCCGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppcs ( XM_021321912.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021177587.1 Fundulus heteroclitus phosphopantothenoylcysteine synthetase (ppcs), mRNA.

Price & Availability↑

CloneID	OFu140663
Clone ID Related Accession (Same CDS sequence)	XM_021321912.1
Accession Version	XM_021321912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	phosphopantothenate--cysteine ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012234382.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCATA GTATGCCTTT AAAGCTAAAA TGCTGCTGTG ACACAAGCAC CAAATCACCC 
CTCTTGATGC TGCCTCTTCT CTTTTATCAG ACCATGGCTG AGCCCCGAAC ATCGTCCATT 
GACGGAAAGC TGGCTGAGGA GTTTGCCGTT CCCGCCCACG TGGAGGAGGT GAGGGAGAAG 
ATGGCCGCTT TCGCCTGCCG CCACGCCGAA GCAGGCCGCA GGGTGGTTCT CATCACCTCA 
GGAGGCACCA AAGTCCCTCT GGAGTCGCGC ACCGTCCGCT TCCTGGATAA CTTCAGCAGC 
GGCAGGCGGG GAGCCTCCTC GGCGGAGTAC TTTATTGACT CGGGCTACGC CGTCATCTTC 
CTGCACCGGC ACCGCTCTCT CTACCCGTAC ACGCGCATGT TCTTAACCAT CAACATGCTG 
GACGCCCTGC AGTTCAGGGG CGGCGAAGGA GCCGGCGAGG TGGTGGTCAA CCAGCAGGTG 
CTCCCGGGCA TCGCCGGGGT CCTGAAGAGG TACAACCAGG TGAAAGACGG CGGGCTTCTT 
CTACCCATCG AGTTCAACAC GCTTTCAGAG TATCTGCATT TACTGAAGGC AGCGGCGCAG 
GCGCTCAGCG CTATAGGATC AAAGGCCATG TTCTACTTGG CTGCAGCCGT GTCAGATTTC 
TACATACCGG CGTCAGAGAT GCCTGAACAC AAAATCCAGT CCTCCAACGG ACCTCTTCAG 
CTCAGCATGA AGATGGTTCC TAAGCTGCTG TCGCCGCTGG TTAAGGACTG GGCGCCTCAG 
GCGTTCGTCA CATCCTTCAA GCTGGAGACC GACTCGGCCA TCCTACTGGA CAGGGCCCGG 
CAGGCTCTGG CCGCTTACAG ACACCAGGCG GTGGTGGCCA ACGTGCTGGA CTCCCGGCGG 
GGCTACGTGG TGGTGGTGAC CCCGGACACC CAGGCAGAGC TGGTCCTCAC CGAGGACGAG 
GCGGAGAAGG ACGTGGAGAT CGAGGAGAGG ATAGTGAGCA ACCTGACGTC GGCGCACAGC 
AAGTTCATAA GTCAACAAGC CGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021177587.1
CDS	3..1049
Translation	MLHSMPLKLKCCCDTSTKSPLLMLPLLFYQTMAEPRTSSIDGKLAEEFAVPAHVEEVREKMAAFACRHAEAGRRVVLITSGGTKVPLESRTVRFLDNFSSGRRGASSAEYFIDSGYAVIFLHRHRSLYPYTRMFLTINMLDALQFRGGEGAGEVVVNQQVLPGIAGVLKRYNQVKDGGLLLPIEFNTLSEYLHLLKAAAQALSAIGSKAMFYLAAAVSDFYIPASEMPEHKIQSSNGPLQLSMKMVPKLLSPLVKDWAPQAFVTSFKLETDSAILLDRARQALAAYRHQAVVANVLDSRRGYVVVVTPDTQAELVLTEDEAEKDVEIEERIVSNLTSAHSKFISQQAG

Target ORF information:

RefSeq Version	XM_021321912.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus phosphopantothenoylcysteine synthetase (ppcs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021321912.1

ORF Insert Sequence:

ATGTTGCATA GTATGCCTTT AAAGCTAAAA TGCTGCTGTG ACACAAGCAC CAAATCACCC 
CTCTTGATGC TGCCTCTTCT CTTTTATCAG ACCATGGCTG AGCCCCGAAC ATCGTCCATT 
GACGGAAAGC TGGCTGAGGA GTTTGCCGTT CCCGCCCACG TGGAGGAGGT GAGGGAGAAG 
ATGGCCGCTT TCGCCTGCCG CCACGCCGAA GCAGGCCGCA GGGTGGTTCT CATCACCTCA 
GGAGGCACCA AAGTCCCTCT GGAGTCGCGC ACCGTCCGCT TCCTGGATAA CTTCAGCAGC 
GGCAGGCGGG GAGCCTCCTC GGCGGAGTAC TTTATTGACT CGGGCTACGC CGTCATCTTC 
CTGCACCGGC ACCGCTCTCT CTACCCGTAC ACGCGCATGT TCTTAACCAT CAACATGCTG 
GACGCCCTGC AGTTCAGGGG CGGCGAAGGA GCCGGCGAGG TGGTGGTCAA CCAGCAGGTG 
CTCCCGGGCA TCGCCGGGGT CCTGAAGAGG TACAACCAGG TGAAAGACGG CGGGCTTCTT 
CTACCCATCG AGTTCAACAC GCTTTCAGAG TATCTGCATT TACTGAAGGC AGCGGCGCAG 
GCGCTCAGCG CTATAGGATC AAAGGCCATG TTCTACTTGG CTGCAGCCGT GTCAGATTTC 
TACATACCGG CGTCAGAGAT GCCTGAACAC AAAATCCAGT CCTCCAACGG ACCTCTTCAG 
CTCAGCATGA AGATGGTTCC TAAGCTGCTG TCGCCGCTGG TTAAGGACTG GGCGCCTCAG 
GCGTTCGTCA CATCCTTCAA GCTGGAGACC GACTCGGCCA TCCTACTGGA CAGGGCCCGG 
CAGGCTCTGG CCGCTTACAG ACACCAGGCG GTGGTGGCCA ACGTGCTGGA CTCCCGGCGG 
GGCTACGTGG TGGTGGTGAC CCCGGACACC CAGGCAGAGC TGGTCCTCAC CGAGGACGAG 
GCGGAGAAGG ACGTGGAGAT CGAGGAGAGG ATAGTGAGCA ACCTGACGTC GGCGCACAGC 
AAGTTCATAA GTCAACAAGC CGGGTGA