stxbp5b cDNA ORF clone, Danio rerio(zebrafish)

The following stxbp5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the stxbp5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa145843 XM_021473077.1
Latest version!
Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $104.30
$149.00
ODa22216 XM_005158604.4
Latest version!
Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa46210 XM_009292821.3
Latest version!
Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa46211 XM_009292822.3
Latest version!
Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa46212 XM_009292823.3
Latest version!
Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa46213 XM_009292824.3
Latest version!
Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa46214 XM_009292825.3
Latest version!
Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa46213 XM_009292824.2 Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa46212 XM_009292823.2 Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa46211 XM_009292822.2 Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa22216 XM_005158604.3 Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa46210 XM_009292821.2 Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa46214 XM_009292825.2 Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa145843
    Clone ID Related Accession (Same CDS sequence) XM_021473077.1
    Accession Version XM_021473077.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product syntaxin-binding protein 5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394976.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTGTATCC TTTTCATATG
    AAGATCTGGC AACTTAACCA GCCCAACTTA CTTGGTGAGG AAAGTGTCAA ATGTGCTACT
    GTTGTTTGTG TATATTTAAA CAAAGGCAAT CCTCCTACCC TTCTGTGTTG GAAGCCTTGT
    TGCACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021328752.1
    CDS334..762
    Translation

    Target ORF information:

    RefSeq Version XM_021473077.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473077.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTGTATCC TTTTCATATG
    AAGATCTGGC AACTTAACCA GCCCAACTTA CTTGGTGAGG AAAGTGTCAA ATGTGCTACT
    GTTGTTTGTG TATATTTAAA CAAAGGCAAT CCTCCTACCC TTCTGTGTTG GAAGCCTTGT
    TGCACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22216
    Clone ID Related Accession (Same CDS sequence) XM_005158604.4 , XM_005158604.3
    Accession Version XM_005158604.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product syntaxin-binding protein 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) and transcript sequence (GFIL01021662.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158604.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 214 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGGTCTT TGTGATGGTA ATGATGCATC AGCTGCCCCA
    GAAGAAAGAT GCAAATCACC AACCTCAGAT GCCAGAGACA ACTCATTCAG CCGCTCGCGC
    AGCTCCAGTG TGACCAGCAT TGACAGAGAG TCACGGGAGG TCATTTCCTC CTTTTACTTT
    TGCGACTCTC TGTCCAAGAA GAGCGAGACG CTGGCAGTAC CCAGTCTGTG GGTGGGCACC
    TCACTGGGCA GCATGTTGGC CATCGCCCTC ACCGTTCCCT CCACACCAGA GCAGAGGATG
    CAACAGCCTG TGGGCGTTTC TTTCTGTGGT TCAGTTGTGC GCTTGAAGGG AGCTATTCTG
    CGTATGGCCC AGCTGGATGC CAGCGGGACT GTCCTTCCTC ATATTTATGA GCCCTGGTAT
    GAGCCCAACG CCCCTGAAGA GGAGAGAGAG CGGCCACGGA GACGCAGACC CACGTCCCCT
    CCGTCATCTC AGGAGAACCA GGACTGTCAG TACGCCGTGG TCTGCTCTGA GAAACAGGCC
    AAGGTGGTGG CGCTGCCCTC CCAACACTGC ATCTTTGAAC ACAACATCAC TGAAGCATCC
    TTTGTGCTTA GAGCCGATGT GGTTACGATG ACGGCCGGGG TTGCCATCGC CTGTTTCTGT
    GCCAACGGCC ACATCATGAC CCTCAGTTTG CCCAGCCTGC GGCCATTGCT GGATGTGAAC
    TACCTTCCTC TGACGGACAT GAGGATAGCC AGAACCTTCT GTTTCTCAAA CCAAGGCCAA
    GCCCTGTACC TCACATCGCC CACTGAGATC CAAAGAATCA CCTATAGCCA GGAAACCTGT
    GAAAACCTGC AGGAGATGCT TGGAGAGATG TTTACTCTAG TGGAGACACC AGAAGCTCCA
    AACAGAGGCT TCTTCAAGGG CTTGTTTGGA GGCGGCGCTC AGTCCTTGGA CAGAGAGGAT
    CTGTTTGGAG AGGTGGCTGC TGGGAAGGCC TCACGCAGCC TAGCCCAGCA CATCCCGGGT
    CCTGGTGGTA TGGAGGGAAT GAAGGGGGCG GCGTCAGGTG TGGTGGGAGA GCTGGCGCGG
    GCGAGGATAG CGCTGGATGA GAGAGGACAG AAACTGGGAG AGCTGGAGGA GAGGACGGCA
    GCCATGATGG CCAGCGCAGA CTCCTTCTCC AAACATGCGC ATGATATGAT GCTGAAATAC
    AAGGACAAGA AATGGTACCA GCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158661.1
    CDS319..3645
    Translation

    Target ORF information:

    RefSeq Version XM_005158604.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158604.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGGTCTT TGTGATGGTA ATGATGCATC AGCTGCCCCA
    GAAGAAAGAT GCAAATCACC AACCTCAGAT GCCAGAGACA ACTCATTCAG CCGCTCGCGC
    AGCTCCAGTG TGACCAGCAT TGACAGAGAG TCACGGGAGG TCATTTCCTC CTTTTACTTT
    TGCGACTCTC TGTCCAAGAA GAGCGAGACG CTGGCAGTAC CCAGTCTGTG GGTGGGCACC
    TCACTGGGCA GCATGTTGGC CATCGCCCTC ACCGTTCCCT CCACACCAGA GCAGAGGATG
    CAACAGCCTG TGGGCGTTTC TTTCTGTGGT TCAGTTGTGC GCTTGAAGGG AGCTATTCTG
    CGTATGGCCC AGCTGGATGC CAGCGGGACT GTCCTTCCTC ATATTTATGA GCCCTGGTAT
    GAGCCCAACG CCCCTGAAGA GGAGAGAGAG CGGCCACGGA GACGCAGACC CACGTCCCCT
    CCGTCATCTC AGGAGAACCA GGACTGTCAG TACGCCGTGG TCTGCTCTGA GAAACAGGCC
    AAGGTGGTGG CGCTGCCCTC CCAACACTGC ATCTTTGAAC ACAACATCAC TGAAGCATCC
    TTTGTGCTTA GAGCCGATGT GGTTACGATG ACGGCCGGGG TTGCCATCGC CTGTTTCTGT
    GCCAACGGCC ACATCATGAC CCTCAGTTTG CCCAGCCTGC GGCCATTGCT GGATGTGAAC
    TACCTTCCTC TGACGGACAT GAGGATAGCC AGAACCTTCT GTTTCTCAAA CCAAGGCCAA
    GCCCTGTACC TCACATCGCC CACTGAGATC CAAAGAATCA CCTATAGCCA GGAAACCTGT
    GAAAACCTGC AGGAGATGCT TGGAGAGATG TTTACTCTAG TGGAGACACC AGAAGCTCCA
    AACAGAGGCT TCTTCAAGGG CTTGTTTGGA GGCGGCGCTC AGTCCTTGGA CAGAGAGGAT
    CTGTTTGGAG AGGTGGCTGC TGGGAAGGCC TCACGCAGCC TAGCCCAGCA CATCCCGGGT
    CCTGGTGGTA TGGAGGGAAT GAAGGGGGCG GCGTCAGGTG TGGTGGGAGA GCTGGCGCGG
    GCGAGGATAG CGCTGGATGA GAGAGGACAG AAACTGGGAG AGCTGGAGGA GAGGACGGCA
    GCCATGATGG CCAGCGCAGA CTCCTTCTCC AAACATGCGC ATGATATGAT GCTGAAATAC
    AAGGACAAGA AATGGTACCA GCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22216
    Clone ID Related Accession (Same CDS sequence) XM_005158604.4 , XM_005158604.3
    Accession Version XM_005158604.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product syntaxin-binding protein 5 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158604.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGGTCTT TGTGATGGTA ATGATGCATC AGCTGCCCCA
    GAAGAAAGAT GCAAATCACC AACCTCAGAT GCCAGAGACA ACTCATTCAG CCGCTCGCGC
    AGCTCCAGTG TGACCAGCAT TGACAGAGAG TCACGGGAGG TCATTTCCTC CTTTTACTTT
    TGCGACTCTC TGTCCAAGAA GAGCGAGACG CTGGCAGTAC CCAGTCTGTG GGTGGGCACC
    TCACTGGGCA GCATGTTGGC CATCGCCCTC ACCGTTCCCT CCACACCAGA GCAGAGGATG
    CAACAGCCTG TGGGCGTTTC TTTCTGTGGT TCAGTTGTGC GCTTGAAGGG AGCTATTCTG
    CGTATGGCCC AGCTGGATGC CAGCGGGACT GTCCTTCCTC ATATTTATGA GCCCTGGTAT
    GAGCCCAACG CCCCTGAAGA GGAGAGAGAG CGGCCACGGA GACGCAGACC CACGTCCCCT
    CCGTCATCTC AGGAGAACCA GGACTGTCAG TACGCCGTGG TCTGCTCTGA GAAACAGGCC
    AAGGTGGTGG CGCTGCCCTC CCAACACTGC ATCTTTGAAC ACAACATCAC TGAAGCATCC
    TTTGTGCTTA GAGCCGATGT GGTTACGATG ACGGCCGGGG TTGCCATCGC CTGTTTCTGT
    GCCAACGGCC ACATCATGAC CCTCAGTTTG CCCAGCCTGC GGCCATTGCT GGATGTGAAC
    TACCTTCCTC TGACGGACAT GAGGATAGCC AGAACCTTCT GTTTCTCAAA CCAAGGCCAA
    GCCCTGTACC TCACATCGCC CACTGAGATC CAAAGAATCA CCTATAGCCA GGAAACCTGT
    GAAAACCTGC AGGAGATGCT TGGAGAGATG TTTACTCTAG TGGAGACACC AGAAGCTCCA
    AACAGAGGCT TCTTCAAGGG CTTGTTTGGA GGCGGCGCTC AGTCCTTGGA CAGAGAGGAT
    CTGTTTGGAG AGGTGGCTGC TGGGAAGGCC TCACGCAGCC TAGCCCAGCA CATCCCGGGT
    CCTGGTGGTA TGGAGGGAAT GAAGGGGGCG GCGTCAGGTG TGGTGGGAGA GCTGGCGCGG
    GCGAGGATAG CGCTGGATGA GAGAGGACAG AAACTGGGAG AGCTGGAGGA GAGGACGGCA
    GCCATGATGG CCAGCGCAGA CTCCTTCTCC AAACATGCGC ATGATATGAT GCTGAAATAC
    AAGGACAAGA AATGGTACCA GCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158661.1
    CDS276..3602
    Translation

    Target ORF information:

    RefSeq Version XM_005158604.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158604.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGGTCTT TGTGATGGTA ATGATGCATC AGCTGCCCCA
    GAAGAAAGAT GCAAATCACC AACCTCAGAT GCCAGAGACA ACTCATTCAG CCGCTCGCGC
    AGCTCCAGTG TGACCAGCAT TGACAGAGAG TCACGGGAGG TCATTTCCTC CTTTTACTTT
    TGCGACTCTC TGTCCAAGAA GAGCGAGACG CTGGCAGTAC CCAGTCTGTG GGTGGGCACC
    TCACTGGGCA GCATGTTGGC CATCGCCCTC ACCGTTCCCT CCACACCAGA GCAGAGGATG
    CAACAGCCTG TGGGCGTTTC TTTCTGTGGT TCAGTTGTGC GCTTGAAGGG AGCTATTCTG
    CGTATGGCCC AGCTGGATGC CAGCGGGACT GTCCTTCCTC ATATTTATGA GCCCTGGTAT
    GAGCCCAACG CCCCTGAAGA GGAGAGAGAG CGGCCACGGA GACGCAGACC CACGTCCCCT
    CCGTCATCTC AGGAGAACCA GGACTGTCAG TACGCCGTGG TCTGCTCTGA GAAACAGGCC
    AAGGTGGTGG CGCTGCCCTC CCAACACTGC ATCTTTGAAC ACAACATCAC TGAAGCATCC
    TTTGTGCTTA GAGCCGATGT GGTTACGATG ACGGCCGGGG TTGCCATCGC CTGTTTCTGT
    GCCAACGGCC ACATCATGAC CCTCAGTTTG CCCAGCCTGC GGCCATTGCT GGATGTGAAC
    TACCTTCCTC TGACGGACAT GAGGATAGCC AGAACCTTCT GTTTCTCAAA CCAAGGCCAA
    GCCCTGTACC TCACATCGCC CACTGAGATC CAAAGAATCA CCTATAGCCA GGAAACCTGT
    GAAAACCTGC AGGAGATGCT TGGAGAGATG TTTACTCTAG TGGAGACACC AGAAGCTCCA
    AACAGAGGCT TCTTCAAGGG CTTGTTTGGA GGCGGCGCTC AGTCCTTGGA CAGAGAGGAT
    CTGTTTGGAG AGGTGGCTGC TGGGAAGGCC TCACGCAGCC TAGCCCAGCA CATCCCGGGT
    CCTGGTGGTA TGGAGGGAAT GAAGGGGGCG GCGTCAGGTG TGGTGGGAGA GCTGGCGCGG
    GCGAGGATAG CGCTGGATGA GAGAGGACAG AAACTGGGAG AGCTGGAGGA GAGGACGGCA
    GCCATGATGG CCAGCGCAGA CTCCTTCTCC AAACATGCGC ATGATATGAT GCTGAAATAC
    AAGGACAAGA AATGGTACCA GCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46210
    Clone ID Related Accession (Same CDS sequence) XM_009292821.3 , XM_009292821.2
    Accession Version XM_009292821.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product syntaxin-binding protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) and transcript sequence (GFIL01021662.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292821.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 214 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGACACT CTGCATAAGA AGTCTTGCAT GTCCGGCCTC
    TCTAACTTTT ATTCTGAATC AGTGAAAAAG CTTCGTTCTT CTTACCTCAG TCTTTGTGAT
    GGTAATGATG CATCAGCTGC CCCAGAAGAA AGATGCAAAT CACCAACCTC AGGCTCCGGT
    TCACCGTGCA ATTCTGATGA AGACAGCAAA CAAAAGTTCA TTGAGAGGGT GAAGACCAAA
    AGCAAGCGGA TATCCAAGAC TGTTGCCAAT GACTTTGCTA AGATATCCCG GAAAATTAAC
    ATGCTTGCTG ATCAGAAAGA TGATGCCAGA GACAACTCAT TCAGCCGCTC GCGCAGCTCC
    AGTGTGACCA GCATTGACAG AGAGTCACGG GAGGTCATTT CCTCCTTTTA CTTTTGCGAC
    TCTCTGTCCA AGAAGAGCGA GACGCTGGCA GTACCCAGTC TGTGGGTGGG CACCTCACTG
    GGCAGCATGT TGGCCATCGC CCTCACCGTT CCCTCCACAC CAGAGCAGAG GATGCAACAG
    CCTGTGGGCG TTTCTTTCTG TGGTTCAGTT GTGCGCTTGA AGGGAGCTAT TCTGCGTATG
    GCCCAGCTGG ATGCCAGCGG GACTGTCCTT CCTCATATTT ATGAGCCCTG GTATGAGCCC
    AACGCCCCTG AAGAGGAGAG AGAGCGGCCA CGGAGACGCA GACCCACGTC CCCTCCGTCA
    TCTCAGGAGA ACCAGGACTG TCAGTACGCC GTGGTCTGCT CTGAGAAACA GGCCAAGGTG
    GTGGCGCTGC CCTCCCAACA CTGCATCTTT GAACACAACA TCACTGAAGC ATCCTTTGTG
    CTTAGAGCCG ATGTGGTTAC GATGACGGCC GGGGTTGCCA TCGCCTGTTT CTGTGCCAAC
    GGCCACATCA TGACCCTCAG TTTGCCCAGC CTGCGGCCAT TGCTGGATGT GAACTACCTT
    CCTCTGACGG ACATGAGGAT AGCCAGAACC TTCTGTTTCT CAAACCAAGG CCAAGCCCTG
    TACCTCACAT CGCCCACTGA GATCCAAAGA ATCACCTATA GCCAGGAAAC CTGTGAAAAC
    CTGCAGGAGA TGCTTGGAGA GATGTTTACT CTAGTGGAGA CACCAGAAGC TCCAAACAGA
    GGCTTCTTCA AGGGCTTGTT TGGAGGCGGC GCTCAGTCCT TGGACAGAGA GGATCTGTTT
    GGAGAGGTGG CTGCTGGGAA GGCCTCACGC AGCCTAGCCC AGCACATCCC GGGTCCTGGT
    GGTATGGAGG GAATGAAGGG GGCGGCGTCA GGTGTGGTGG GAGAGCTGGC GCGGGCGAGG
    ATAGCGCTGG ATGAGAGAGG ACAGAAACTG GGAGAGCTGG AGGAGAGGAC GGCAGCCATG
    ATGGCCAGCG CAGACTCCTT CTCCAAACAT GCGCATGATA TGATGCTGAA ATACAAGGAC
    AAGAAATGGT ACCAGCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291096.1
    CDS321..3881
    Translation

    Target ORF information:

    RefSeq Version XM_009292821.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292821.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGACACT CTGCATAAGA AGTCTTGCAT GTCCGGCCTC
    TCTAACTTTT ATTCTGAATC AGTGAAAAAG CTTCGTTCTT CTTACCTCAG TCTTTGTGAT
    GGTAATGATG CATCAGCTGC CCCAGAAGAA AGATGCAAAT CACCAACCTC AGGCTCCGGT
    TCACCGTGCA ATTCTGATGA AGACAGCAAA CAAAAGTTCA TTGAGAGGGT GAAGACCAAA
    AGCAAGCGGA TATCCAAGAC TGTTGCCAAT GACTTTGCTA AGATATCCCG GAAAATTAAC
    ATGCTTGCTG ATCAGAAAGA TGATGCCAGA GACAACTCAT TCAGCCGCTC GCGCAGCTCC
    AGTGTGACCA GCATTGACAG AGAGTCACGG GAGGTCATTT CCTCCTTTTA CTTTTGCGAC
    TCTCTGTCCA AGAAGAGCGA GACGCTGGCA GTACCCAGTC TGTGGGTGGG CACCTCACTG
    GGCAGCATGT TGGCCATCGC CCTCACCGTT CCCTCCACAC CAGAGCAGAG GATGCAACAG
    CCTGTGGGCG TTTCTTTCTG TGGTTCAGTT GTGCGCTTGA AGGGAGCTAT TCTGCGTATG
    GCCCAGCTGG ATGCCAGCGG GACTGTCCTT CCTCATATTT ATGAGCCCTG GTATGAGCCC
    AACGCCCCTG AAGAGGAGAG AGAGCGGCCA CGGAGACGCA GACCCACGTC CCCTCCGTCA
    TCTCAGGAGA ACCAGGACTG TCAGTACGCC GTGGTCTGCT CTGAGAAACA GGCCAAGGTG
    GTGGCGCTGC CCTCCCAACA CTGCATCTTT GAACACAACA TCACTGAAGC ATCCTTTGTG
    CTTAGAGCCG ATGTGGTTAC GATGACGGCC GGGGTTGCCA TCGCCTGTTT CTGTGCCAAC
    GGCCACATCA TGACCCTCAG TTTGCCCAGC CTGCGGCCAT TGCTGGATGT GAACTACCTT
    CCTCTGACGG ACATGAGGAT AGCCAGAACC TTCTGTTTCT CAAACCAAGG CCAAGCCCTG
    TACCTCACAT CGCCCACTGA GATCCAAAGA ATCACCTATA GCCAGGAAAC CTGTGAAAAC
    CTGCAGGAGA TGCTTGGAGA GATGTTTACT CTAGTGGAGA CACCAGAAGC TCCAAACAGA
    GGCTTCTTCA AGGGCTTGTT TGGAGGCGGC GCTCAGTCCT TGGACAGAGA GGATCTGTTT
    GGAGAGGTGG CTGCTGGGAA GGCCTCACGC AGCCTAGCCC AGCACATCCC GGGTCCTGGT
    GGTATGGAGG GAATGAAGGG GGCGGCGTCA GGTGTGGTGG GAGAGCTGGC GCGGGCGAGG
    ATAGCGCTGG ATGAGAGAGG ACAGAAACTG GGAGAGCTGG AGGAGAGGAC GGCAGCCATG
    ATGGCCAGCG CAGACTCCTT CTCCAAACAT GCGCATGATA TGATGCTGAA ATACAAGGAC
    AAGAAATGGT ACCAGCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46210
    Clone ID Related Accession (Same CDS sequence) XM_009292821.3 , XM_009292821.2
    Accession Version XM_009292821.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product syntaxin-binding protein 5 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292821.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGACACT CTGCATAAGA AGTCTTGCAT GTCCGGCCTC
    TCTAACTTTT ATTCTGAATC AGTGAAAAAG CTTCGTTCTT CTTACCTCAG TCTTTGTGAT
    GGTAATGATG CATCAGCTGC CCCAGAAGAA AGATGCAAAT CACCAACCTC AGGCTCCGGT
    TCACCGTGCA ATTCTGATGA AGACAGCAAA CAAAAGTTCA TTGAGAGGGT GAAGACCAAA
    AGCAAGCGGA TATCCAAGAC TGTTGCCAAT GACTTTGCTA AGATATCCCG GAAAATTAAC
    ATGCTTGCTG ATCAGAAAGA TGATGCCAGA GACAACTCAT TCAGCCGCTC GCGCAGCTCC
    AGTGTGACCA GCATTGACAG AGAGTCACGG GAGGTCATTT CCTCCTTTTA CTTTTGCGAC
    TCTCTGTCCA AGAAGAGCGA GACGCTGGCA GTACCCAGTC TGTGGGTGGG CACCTCACTG
    GGCAGCATGT TGGCCATCGC CCTCACCGTT CCCTCCACAC CAGAGCAGAG GATGCAACAG
    CCTGTGGGCG TTTCTTTCTG TGGTTCAGTT GTGCGCTTGA AGGGAGCTAT TCTGCGTATG
    GCCCAGCTGG ATGCCAGCGG GACTGTCCTT CCTCATATTT ATGAGCCCTG GTATGAGCCC
    AACGCCCCTG AAGAGGAGAG AGAGCGGCCA CGGAGACGCA GACCCACGTC CCCTCCGTCA
    TCTCAGGAGA ACCAGGACTG TCAGTACGCC GTGGTCTGCT CTGAGAAACA GGCCAAGGTG
    GTGGCGCTGC CCTCCCAACA CTGCATCTTT GAACACAACA TCACTGAAGC ATCCTTTGTG
    CTTAGAGCCG ATGTGGTTAC GATGACGGCC GGGGTTGCCA TCGCCTGTTT CTGTGCCAAC
    GGCCACATCA TGACCCTCAG TTTGCCCAGC CTGCGGCCAT TGCTGGATGT GAACTACCTT
    CCTCTGACGG ACATGAGGAT AGCCAGAACC TTCTGTTTCT CAAACCAAGG CCAAGCCCTG
    TACCTCACAT CGCCCACTGA GATCCAAAGA ATCACCTATA GCCAGGAAAC CTGTGAAAAC
    CTGCAGGAGA TGCTTGGAGA GATGTTTACT CTAGTGGAGA CACCAGAAGC TCCAAACAGA
    GGCTTCTTCA AGGGCTTGTT TGGAGGCGGC GCTCAGTCCT TGGACAGAGA GGATCTGTTT
    GGAGAGGTGG CTGCTGGGAA GGCCTCACGC AGCCTAGCCC AGCACATCCC GGGTCCTGGT
    GGTATGGAGG GAATGAAGGG GGCGGCGTCA GGTGTGGTGG GAGAGCTGGC GCGGGCGAGG
    ATAGCGCTGG ATGAGAGAGG ACAGAAACTG GGAGAGCTGG AGGAGAGGAC GGCAGCCATG
    ATGGCCAGCG CAGACTCCTT CTCCAAACAT GCGCATGATA TGATGCTGAA ATACAAGGAC
    AAGAAATGGT ACCAGCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291096.1
    CDS276..3836
    Translation

    Target ORF information:

    RefSeq Version XM_009292821.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292821.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGACACT CTGCATAAGA AGTCTTGCAT GTCCGGCCTC
    TCTAACTTTT ATTCTGAATC AGTGAAAAAG CTTCGTTCTT CTTACCTCAG TCTTTGTGAT
    GGTAATGATG CATCAGCTGC CCCAGAAGAA AGATGCAAAT CACCAACCTC AGGCTCCGGT
    TCACCGTGCA ATTCTGATGA AGACAGCAAA CAAAAGTTCA TTGAGAGGGT GAAGACCAAA
    AGCAAGCGGA TATCCAAGAC TGTTGCCAAT GACTTTGCTA AGATATCCCG GAAAATTAAC
    ATGCTTGCTG ATCAGAAAGA TGATGCCAGA GACAACTCAT TCAGCCGCTC GCGCAGCTCC
    AGTGTGACCA GCATTGACAG AGAGTCACGG GAGGTCATTT CCTCCTTTTA CTTTTGCGAC
    TCTCTGTCCA AGAAGAGCGA GACGCTGGCA GTACCCAGTC TGTGGGTGGG CACCTCACTG
    GGCAGCATGT TGGCCATCGC CCTCACCGTT CCCTCCACAC CAGAGCAGAG GATGCAACAG
    CCTGTGGGCG TTTCTTTCTG TGGTTCAGTT GTGCGCTTGA AGGGAGCTAT TCTGCGTATG
    GCCCAGCTGG ATGCCAGCGG GACTGTCCTT CCTCATATTT ATGAGCCCTG GTATGAGCCC
    AACGCCCCTG AAGAGGAGAG AGAGCGGCCA CGGAGACGCA GACCCACGTC CCCTCCGTCA
    TCTCAGGAGA ACCAGGACTG TCAGTACGCC GTGGTCTGCT CTGAGAAACA GGCCAAGGTG
    GTGGCGCTGC CCTCCCAACA CTGCATCTTT GAACACAACA TCACTGAAGC ATCCTTTGTG
    CTTAGAGCCG ATGTGGTTAC GATGACGGCC GGGGTTGCCA TCGCCTGTTT CTGTGCCAAC
    GGCCACATCA TGACCCTCAG TTTGCCCAGC CTGCGGCCAT TGCTGGATGT GAACTACCTT
    CCTCTGACGG ACATGAGGAT AGCCAGAACC TTCTGTTTCT CAAACCAAGG CCAAGCCCTG
    TACCTCACAT CGCCCACTGA GATCCAAAGA ATCACCTATA GCCAGGAAAC CTGTGAAAAC
    CTGCAGGAGA TGCTTGGAGA GATGTTTACT CTAGTGGAGA CACCAGAAGC TCCAAACAGA
    GGCTTCTTCA AGGGCTTGTT TGGAGGCGGC GCTCAGTCCT TGGACAGAGA GGATCTGTTT
    GGAGAGGTGG CTGCTGGGAA GGCCTCACGC AGCCTAGCCC AGCACATCCC GGGTCCTGGT
    GGTATGGAGG GAATGAAGGG GGCGGCGTCA GGTGTGGTGG GAGAGCTGGC GCGGGCGAGG
    ATAGCGCTGG ATGAGAGAGG ACAGAAACTG GGAGAGCTGG AGGAGAGGAC GGCAGCCATG
    ATGGCCAGCG CAGACTCCTT CTCCAAACAT GCGCATGATA TGATGCTGAA ATACAAGGAC
    AAGAAATGGT ACCAGCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46211
    Clone ID Related Accession (Same CDS sequence) XM_009292822.3 , XM_009292822.2
    Accession Version XM_009292822.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product syntaxin-binding protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) and transcript sequence (GFIL01021662.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292822.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 214 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGGTCTT TGTGATGGTA ATGATGCATC AGCTGCCCCA
    GAAGAAAGAT GCAAATCACC AACCTCAGGC TCCGGTTCAC CGTGCAATTC TGATGAAGAC
    AGCAAACAAA AGTTCATTGA GAGGGTGAAG ACCAAAAGCA AGCGGATATC CAAGACTGTT
    GCCAATGACT TTGCTAAGAT ATCCCGGAAA ATTAACATGC TTGCTGATCA GAAAGATGAT
    GCCAGAGACA ACTCATTCAG CCGCTCGCGC AGCTCCAGTG TGACCAGCAT TGACAGAGAG
    TCACGGGAGG TCATTTCCTC CTTTTACTTT TGCGACTCTC TGTCCAAGAA GAGCGAGACG
    CTGGCAGTAC CCAGTCTGTG GGTGGGCACC TCACTGGGCA GCATGTTGGC CATCGCCCTC
    ACCGTTCCCT CCACACCAGA GCAGAGGATG CAACAGCCTG TGGGCGTTTC TTTCTGTGGT
    TCAGTTGTGC GCTTGAAGGG AGCTATTCTG CGTATGGCCC AGCTGGATGC CAGCGGGACT
    GTCCTTCCTC ATATTTATGA GCCCTGGTAT GAGCCCAACG CCCCTGAAGA GGAGAGAGAG
    CGGCCACGGA GACGCAGACC CACGTCCCCT CCGTCATCTC AGGAGAACCA GGACTGTCAG
    TACGCCGTGG TCTGCTCTGA GAAACAGGCC AAGGTGGTGG CGCTGCCCTC CCAACACTGC
    ATCTTTGAAC ACAACATCAC TGAAGCATCC TTTGTGCTTA GAGCCGATGT GGTTACGATG
    ACGGCCGGGG TTGCCATCGC CTGTTTCTGT GCCAACGGCC ACATCATGAC CCTCAGTTTG
    CCCAGCCTGC GGCCATTGCT GGATGTGAAC TACCTTCCTC TGACGGACAT GAGGATAGCC
    AGAACCTTCT GTTTCTCAAA CCAAGGCCAA GCCCTGTACC TCACATCGCC CACTGAGATC
    CAAAGAATCA CCTATAGCCA GGAAACCTGT GAAAACCTGC AGGAGATGCT TGGAGAGATG
    TTTACTCTAG TGGAGACACC AGAAGCTCCA AACAGAGGCT TCTTCAAGGG CTTGTTTGGA
    GGCGGCGCTC AGTCCTTGGA CAGAGAGGAT CTGTTTGGAG AGGTGGCTGC TGGGAAGGCC
    TCACGCAGCC TAGCCCAGCA CATCCCGGGT CCTGGTGGTA TGGAGGGAAT GAAGGGGGCG
    GCGTCAGGTG TGGTGGGAGA GCTGGCGCGG GCGAGGATAG CGCTGGATGA GAGAGGACAG
    AAACTGGGAG AGCTGGAGGA GAGGACGGCA GCCATGATGG CCAGCGCAGA CTCCTTCTCC
    AAACATGCGC ATGATATGAT GCTGAAATAC AAGGACAAGA AATGGTACCA GCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291097.1
    CDS320..3796
    Translation

    Target ORF information:

    RefSeq Version XM_009292822.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292822.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGGTCTT TGTGATGGTA ATGATGCATC AGCTGCCCCA
    GAAGAAAGAT GCAAATCACC AACCTCAGGC TCCGGTTCAC CGTGCAATTC TGATGAAGAC
    AGCAAACAAA AGTTCATTGA GAGGGTGAAG ACCAAAAGCA AGCGGATATC CAAGACTGTT
    GCCAATGACT TTGCTAAGAT ATCCCGGAAA ATTAACATGC TTGCTGATCA GAAAGATGAT
    GCCAGAGACA ACTCATTCAG CCGCTCGCGC AGCTCCAGTG TGACCAGCAT TGACAGAGAG
    TCACGGGAGG TCATTTCCTC CTTTTACTTT TGCGACTCTC TGTCCAAGAA GAGCGAGACG
    CTGGCAGTAC CCAGTCTGTG GGTGGGCACC TCACTGGGCA GCATGTTGGC CATCGCCCTC
    ACCGTTCCCT CCACACCAGA GCAGAGGATG CAACAGCCTG TGGGCGTTTC TTTCTGTGGT
    TCAGTTGTGC GCTTGAAGGG AGCTATTCTG CGTATGGCCC AGCTGGATGC CAGCGGGACT
    GTCCTTCCTC ATATTTATGA GCCCTGGTAT GAGCCCAACG CCCCTGAAGA GGAGAGAGAG
    CGGCCACGGA GACGCAGACC CACGTCCCCT CCGTCATCTC AGGAGAACCA GGACTGTCAG
    TACGCCGTGG TCTGCTCTGA GAAACAGGCC AAGGTGGTGG CGCTGCCCTC CCAACACTGC
    ATCTTTGAAC ACAACATCAC TGAAGCATCC TTTGTGCTTA GAGCCGATGT GGTTACGATG
    ACGGCCGGGG TTGCCATCGC CTGTTTCTGT GCCAACGGCC ACATCATGAC CCTCAGTTTG
    CCCAGCCTGC GGCCATTGCT GGATGTGAAC TACCTTCCTC TGACGGACAT GAGGATAGCC
    AGAACCTTCT GTTTCTCAAA CCAAGGCCAA GCCCTGTACC TCACATCGCC CACTGAGATC
    CAAAGAATCA CCTATAGCCA GGAAACCTGT GAAAACCTGC AGGAGATGCT TGGAGAGATG
    TTTACTCTAG TGGAGACACC AGAAGCTCCA AACAGAGGCT TCTTCAAGGG CTTGTTTGGA
    GGCGGCGCTC AGTCCTTGGA CAGAGAGGAT CTGTTTGGAG AGGTGGCTGC TGGGAAGGCC
    TCACGCAGCC TAGCCCAGCA CATCCCGGGT CCTGGTGGTA TGGAGGGAAT GAAGGGGGCG
    GCGTCAGGTG TGGTGGGAGA GCTGGCGCGG GCGAGGATAG CGCTGGATGA GAGAGGACAG
    AAACTGGGAG AGCTGGAGGA GAGGACGGCA GCCATGATGG CCAGCGCAGA CTCCTTCTCC
    AAACATGCGC ATGATATGAT GCTGAAATAC AAGGACAAGA AATGGTACCA GCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46211
    Clone ID Related Accession (Same CDS sequence) XM_009292822.3 , XM_009292822.2
    Accession Version XM_009292822.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product syntaxin-binding protein 5 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292822.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGGTCTT TGTGATGGTA ATGATGCATC AGCTGCCCCA
    GAAGAAAGAT GCAAATCACC AACCTCAGGC TCCGGTTCAC CGTGCAATTC TGATGAAGAC
    AGCAAACAAA AGTTCATTGA GAGGGTGAAG ACCAAAAGCA AGCGGATATC CAAGACTGTT
    GCCAATGACT TTGCTAAGAT ATCCCGGAAA ATTAACATGC TTGCTGATCA GAAAGATGAT
    GCCAGAGACA ACTCATTCAG CCGCTCGCGC AGCTCCAGTG TGACCAGCAT TGACAGAGAG
    TCACGGGAGG TCATTTCCTC CTTTTACTTT TGCGACTCTC TGTCCAAGAA GAGCGAGACG
    CTGGCAGTAC CCAGTCTGTG GGTGGGCACC TCACTGGGCA GCATGTTGGC CATCGCCCTC
    ACCGTTCCCT CCACACCAGA GCAGAGGATG CAACAGCCTG TGGGCGTTTC TTTCTGTGGT
    TCAGTTGTGC GCTTGAAGGG AGCTATTCTG CGTATGGCCC AGCTGGATGC CAGCGGGACT
    GTCCTTCCTC ATATTTATGA GCCCTGGTAT GAGCCCAACG CCCCTGAAGA GGAGAGAGAG
    CGGCCACGGA GACGCAGACC CACGTCCCCT CCGTCATCTC AGGAGAACCA GGACTGTCAG
    TACGCCGTGG TCTGCTCTGA GAAACAGGCC AAGGTGGTGG CGCTGCCCTC CCAACACTGC
    ATCTTTGAAC ACAACATCAC TGAAGCATCC TTTGTGCTTA GAGCCGATGT GGTTACGATG
    ACGGCCGGGG TTGCCATCGC CTGTTTCTGT GCCAACGGCC ACATCATGAC CCTCAGTTTG
    CCCAGCCTGC GGCCATTGCT GGATGTGAAC TACCTTCCTC TGACGGACAT GAGGATAGCC
    AGAACCTTCT GTTTCTCAAA CCAAGGCCAA GCCCTGTACC TCACATCGCC CACTGAGATC
    CAAAGAATCA CCTATAGCCA GGAAACCTGT GAAAACCTGC AGGAGATGCT TGGAGAGATG
    TTTACTCTAG TGGAGACACC AGAAGCTCCA AACAGAGGCT TCTTCAAGGG CTTGTTTGGA
    GGCGGCGCTC AGTCCTTGGA CAGAGAGGAT CTGTTTGGAG AGGTGGCTGC TGGGAAGGCC
    TCACGCAGCC TAGCCCAGCA CATCCCGGGT CCTGGTGGTA TGGAGGGAAT GAAGGGGGCG
    GCGTCAGGTG TGGTGGGAGA GCTGGCGCGG GCGAGGATAG CGCTGGATGA GAGAGGACAG
    AAACTGGGAG AGCTGGAGGA GAGGACGGCA GCCATGATGG CCAGCGCAGA CTCCTTCTCC
    AAACATGCGC ATGATATGAT GCTGAAATAC AAGGACAAGA AATGGTACCA GCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291097.1
    CDS276..3752
    Translation

    Target ORF information:

    RefSeq Version XM_009292822.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292822.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGGTCTT TGTGATGGTA ATGATGCATC AGCTGCCCCA
    GAAGAAAGAT GCAAATCACC AACCTCAGGC TCCGGTTCAC CGTGCAATTC TGATGAAGAC
    AGCAAACAAA AGTTCATTGA GAGGGTGAAG ACCAAAAGCA AGCGGATATC CAAGACTGTT
    GCCAATGACT TTGCTAAGAT ATCCCGGAAA ATTAACATGC TTGCTGATCA GAAAGATGAT
    GCCAGAGACA ACTCATTCAG CCGCTCGCGC AGCTCCAGTG TGACCAGCAT TGACAGAGAG
    TCACGGGAGG TCATTTCCTC CTTTTACTTT TGCGACTCTC TGTCCAAGAA GAGCGAGACG
    CTGGCAGTAC CCAGTCTGTG GGTGGGCACC TCACTGGGCA GCATGTTGGC CATCGCCCTC
    ACCGTTCCCT CCACACCAGA GCAGAGGATG CAACAGCCTG TGGGCGTTTC TTTCTGTGGT
    TCAGTTGTGC GCTTGAAGGG AGCTATTCTG CGTATGGCCC AGCTGGATGC CAGCGGGACT
    GTCCTTCCTC ATATTTATGA GCCCTGGTAT GAGCCCAACG CCCCTGAAGA GGAGAGAGAG
    CGGCCACGGA GACGCAGACC CACGTCCCCT CCGTCATCTC AGGAGAACCA GGACTGTCAG
    TACGCCGTGG TCTGCTCTGA GAAACAGGCC AAGGTGGTGG CGCTGCCCTC CCAACACTGC
    ATCTTTGAAC ACAACATCAC TGAAGCATCC TTTGTGCTTA GAGCCGATGT GGTTACGATG
    ACGGCCGGGG TTGCCATCGC CTGTTTCTGT GCCAACGGCC ACATCATGAC CCTCAGTTTG
    CCCAGCCTGC GGCCATTGCT GGATGTGAAC TACCTTCCTC TGACGGACAT GAGGATAGCC
    AGAACCTTCT GTTTCTCAAA CCAAGGCCAA GCCCTGTACC TCACATCGCC CACTGAGATC
    CAAAGAATCA CCTATAGCCA GGAAACCTGT GAAAACCTGC AGGAGATGCT TGGAGAGATG
    TTTACTCTAG TGGAGACACC AGAAGCTCCA AACAGAGGCT TCTTCAAGGG CTTGTTTGGA
    GGCGGCGCTC AGTCCTTGGA CAGAGAGGAT CTGTTTGGAG AGGTGGCTGC TGGGAAGGCC
    TCACGCAGCC TAGCCCAGCA CATCCCGGGT CCTGGTGGTA TGGAGGGAAT GAAGGGGGCG
    GCGTCAGGTG TGGTGGGAGA GCTGGCGCGG GCGAGGATAG CGCTGGATGA GAGAGGACAG
    AAACTGGGAG AGCTGGAGGA GAGGACGGCA GCCATGATGG CCAGCGCAGA CTCCTTCTCC
    AAACATGCGC ATGATATGAT GCTGAAATAC AAGGACAAGA AATGGTACCA GCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46212
    Clone ID Related Accession (Same CDS sequence) XM_009292823.2 , XM_009292823.3
    Accession Version XM_009292823.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product syntaxin-binding protein 5 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292823.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGACACT CTGCATAAGA AGTCTTGCAT GTCCGGCCTC
    TCTAACTTTT ATTCTGAATC AGTGAAAAAG CTTCGTTCTT CTTACCTCAG TCTTTGTGAT
    GGTAATGATG CATCAGCTGC CCCAGAAGAA AGATGCAAAT CACCAACCTC AGCTAAGATA
    TCCCGGAAAA TTAACATGCT TGCTGATCAG AAAGATGATG CCAGAGACAA CTCATTCAGC
    CGCTCGCGCA GCTCCAGTGT GACCAGCATT GACAGAGAGT CACGGGAGGT CATTTCCTCC
    TTTTACTTTT GCGACTCTCT GTCCAAGAAG AGCGAGACGC TGGCAGTACC CAGTCTGTGG
    GTGGGCACCT CACTGGGCAG CATGTTGGCC ATCGCCCTCA CCGTTCCCTC CACACCAGAG
    CAGAGGATGC AACAGCCTGT GGGCGTTTCT TTCTGTGGTT CAGTTGTGCG CTTGAAGGGA
    GCTATTCTGC GTATGGCCCA GCTGGATGCC AGCGGGACTG TCCTTCCTCA TATTTATGAG
    CCCTGGTATG AGCCCAACGC CCCTGAAGAG GAGAGAGAGC GGCCACGGAG ACGCAGACCC
    ACGTCCCCTC CGTCATCTCA GGAGAACCAG GACTGTCAGT ACGCCGTGGT CTGCTCTGAG
    AAACAGGCCA AGGTGGTGGC GCTGCCCTCC CAACACTGCA TCTTTGAACA CAACATCACT
    GAAGCATCCT TTGTGCTTAG AGCCGATGTG GTTACGATGA CGGCCGGGGT TGCCATCGCC
    TGTTTCTGTG CCAACGGCCA CATCATGACC CTCAGTTTGC CCAGCCTGCG GCCATTGCTG
    GATGTGAACT ACCTTCCTCT GACGGACATG AGGATAGCCA GAACCTTCTG TTTCTCAAAC
    CAAGGCCAAG CCCTGTACCT CACATCGCCC ACTGAGATCC AAAGAATCAC CTATAGCCAG
    GAAACCTGTG AAAACCTGCA GGAGATGCTT GGAGAGATGT TTACTCTAGT GGAGACACCA
    GAAGCTCCAA ACAGAGGCTT CTTCAAGGGC TTGTTTGGAG GCGGCGCTCA GTCCTTGGAC
    AGAGAGGATC TGTTTGGAGA GGTGGCTGCT GGGAAGGCCT CACGCAGCCT AGCCCAGCAC
    ATCCCGGGTC CTGGTGGTAT GGAGGGAATG AAGGGGGCGG CGTCAGGTGT GGTGGGAGAG
    CTGGCGCGGG CGAGGATAGC GCTGGATGAG AGAGGACAGA AACTGGGAGA GCTGGAGGAG
    AGGACGGCAG CCATGATGGC CAGCGCAGAC TCCTTCTCCA AACATGCGCA TGATATGATG
    CTGAAATACA AGGACAAGAA ATGGTACCAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291098.1
    CDS276..3731
    Translation

    Target ORF information:

    RefSeq Version XM_009292823.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292823.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGACACT CTGCATAAGA AGTCTTGCAT GTCCGGCCTC
    TCTAACTTTT ATTCTGAATC AGTGAAAAAG CTTCGTTCTT CTTACCTCAG TCTTTGTGAT
    GGTAATGATG CATCAGCTGC CCCAGAAGAA AGATGCAAAT CACCAACCTC AGCTAAGATA
    TCCCGGAAAA TTAACATGCT TGCTGATCAG AAAGATGATG CCAGAGACAA CTCATTCAGC
    CGCTCGCGCA GCTCCAGTGT GACCAGCATT GACAGAGAGT CACGGGAGGT CATTTCCTCC
    TTTTACTTTT GCGACTCTCT GTCCAAGAAG AGCGAGACGC TGGCAGTACC CAGTCTGTGG
    GTGGGCACCT CACTGGGCAG CATGTTGGCC ATCGCCCTCA CCGTTCCCTC CACACCAGAG
    CAGAGGATGC AACAGCCTGT GGGCGTTTCT TTCTGTGGTT CAGTTGTGCG CTTGAAGGGA
    GCTATTCTGC GTATGGCCCA GCTGGATGCC AGCGGGACTG TCCTTCCTCA TATTTATGAG
    CCCTGGTATG AGCCCAACGC CCCTGAAGAG GAGAGAGAGC GGCCACGGAG ACGCAGACCC
    ACGTCCCCTC CGTCATCTCA GGAGAACCAG GACTGTCAGT ACGCCGTGGT CTGCTCTGAG
    AAACAGGCCA AGGTGGTGGC GCTGCCCTCC CAACACTGCA TCTTTGAACA CAACATCACT
    GAAGCATCCT TTGTGCTTAG AGCCGATGTG GTTACGATGA CGGCCGGGGT TGCCATCGCC
    TGTTTCTGTG CCAACGGCCA CATCATGACC CTCAGTTTGC CCAGCCTGCG GCCATTGCTG
    GATGTGAACT ACCTTCCTCT GACGGACATG AGGATAGCCA GAACCTTCTG TTTCTCAAAC
    CAAGGCCAAG CCCTGTACCT CACATCGCCC ACTGAGATCC AAAGAATCAC CTATAGCCAG
    GAAACCTGTG AAAACCTGCA GGAGATGCTT GGAGAGATGT TTACTCTAGT GGAGACACCA
    GAAGCTCCAA ACAGAGGCTT CTTCAAGGGC TTGTTTGGAG GCGGCGCTCA GTCCTTGGAC
    AGAGAGGATC TGTTTGGAGA GGTGGCTGCT GGGAAGGCCT CACGCAGCCT AGCCCAGCAC
    ATCCCGGGTC CTGGTGGTAT GGAGGGAATG AAGGGGGCGG CGTCAGGTGT GGTGGGAGAG
    CTGGCGCGGG CGAGGATAGC GCTGGATGAG AGAGGACAGA AACTGGGAGA GCTGGAGGAG
    AGGACGGCAG CCATGATGGC CAGCGCAGAC TCCTTCTCCA AACATGCGCA TGATATGATG
    CTGAAATACA AGGACAAGAA ATGGTACCAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46212
    Clone ID Related Accession (Same CDS sequence) XM_009292823.2 , XM_009292823.3
    Accession Version XM_009292823.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product syntaxin-binding protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) and transcript sequence (GFIL01021662.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292823.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 214 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGACACT CTGCATAAGA AGTCTTGCAT GTCCGGCCTC
    TCTAACTTTT ATTCTGAATC AGTGAAAAAG CTTCGTTCTT CTTACCTCAG TCTTTGTGAT
    GGTAATGATG CATCAGCTGC CCCAGAAGAA AGATGCAAAT CACCAACCTC AGCTAAGATA
    TCCCGGAAAA TTAACATGCT TGCTGATCAG AAAGATGATG CCAGAGACAA CTCATTCAGC
    CGCTCGCGCA GCTCCAGTGT GACCAGCATT GACAGAGAGT CACGGGAGGT CATTTCCTCC
    TTTTACTTTT GCGACTCTCT GTCCAAGAAG AGCGAGACGC TGGCAGTACC CAGTCTGTGG
    GTGGGCACCT CACTGGGCAG CATGTTGGCC ATCGCCCTCA CCGTTCCCTC CACACCAGAG
    CAGAGGATGC AACAGCCTGT GGGCGTTTCT TTCTGTGGTT CAGTTGTGCG CTTGAAGGGA
    GCTATTCTGC GTATGGCCCA GCTGGATGCC AGCGGGACTG TCCTTCCTCA TATTTATGAG
    CCCTGGTATG AGCCCAACGC CCCTGAAGAG GAGAGAGAGC GGCCACGGAG ACGCAGACCC
    ACGTCCCCTC CGTCATCTCA GGAGAACCAG GACTGTCAGT ACGCCGTGGT CTGCTCTGAG
    AAACAGGCCA AGGTGGTGGC GCTGCCCTCC CAACACTGCA TCTTTGAACA CAACATCACT
    GAAGCATCCT TTGTGCTTAG AGCCGATGTG GTTACGATGA CGGCCGGGGT TGCCATCGCC
    TGTTTCTGTG CCAACGGCCA CATCATGACC CTCAGTTTGC CCAGCCTGCG GCCATTGCTG
    GATGTGAACT ACCTTCCTCT GACGGACATG AGGATAGCCA GAACCTTCTG TTTCTCAAAC
    CAAGGCCAAG CCCTGTACCT CACATCGCCC ACTGAGATCC AAAGAATCAC CTATAGCCAG
    GAAACCTGTG AAAACCTGCA GGAGATGCTT GGAGAGATGT TTACTCTAGT GGAGACACCA
    GAAGCTCCAA ACAGAGGCTT CTTCAAGGGC TTGTTTGGAG GCGGCGCTCA GTCCTTGGAC
    AGAGAGGATC TGTTTGGAGA GGTGGCTGCT GGGAAGGCCT CACGCAGCCT AGCCCAGCAC
    ATCCCGGGTC CTGGTGGTAT GGAGGGAATG AAGGGGGCGG CGTCAGGTGT GGTGGGAGAG
    CTGGCGCGGG CGAGGATAGC GCTGGATGAG AGAGGACAGA AACTGGGAGA GCTGGAGGAG
    AGGACGGCAG CCATGATGGC CAGCGCAGAC TCCTTCTCCA AACATGCGCA TGATATGATG
    CTGAAATACA AGGACAAGAA ATGGTACCAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291098.1
    CDS320..3775
    Translation

    Target ORF information:

    RefSeq Version XM_009292823.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292823.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGACACT CTGCATAAGA AGTCTTGCAT GTCCGGCCTC
    TCTAACTTTT ATTCTGAATC AGTGAAAAAG CTTCGTTCTT CTTACCTCAG TCTTTGTGAT
    GGTAATGATG CATCAGCTGC CCCAGAAGAA AGATGCAAAT CACCAACCTC AGCTAAGATA
    TCCCGGAAAA TTAACATGCT TGCTGATCAG AAAGATGATG CCAGAGACAA CTCATTCAGC
    CGCTCGCGCA GCTCCAGTGT GACCAGCATT GACAGAGAGT CACGGGAGGT CATTTCCTCC
    TTTTACTTTT GCGACTCTCT GTCCAAGAAG AGCGAGACGC TGGCAGTACC CAGTCTGTGG
    GTGGGCACCT CACTGGGCAG CATGTTGGCC ATCGCCCTCA CCGTTCCCTC CACACCAGAG
    CAGAGGATGC AACAGCCTGT GGGCGTTTCT TTCTGTGGTT CAGTTGTGCG CTTGAAGGGA
    GCTATTCTGC GTATGGCCCA GCTGGATGCC AGCGGGACTG TCCTTCCTCA TATTTATGAG
    CCCTGGTATG AGCCCAACGC CCCTGAAGAG GAGAGAGAGC GGCCACGGAG ACGCAGACCC
    ACGTCCCCTC CGTCATCTCA GGAGAACCAG GACTGTCAGT ACGCCGTGGT CTGCTCTGAG
    AAACAGGCCA AGGTGGTGGC GCTGCCCTCC CAACACTGCA TCTTTGAACA CAACATCACT
    GAAGCATCCT TTGTGCTTAG AGCCGATGTG GTTACGATGA CGGCCGGGGT TGCCATCGCC
    TGTTTCTGTG CCAACGGCCA CATCATGACC CTCAGTTTGC CCAGCCTGCG GCCATTGCTG
    GATGTGAACT ACCTTCCTCT GACGGACATG AGGATAGCCA GAACCTTCTG TTTCTCAAAC
    CAAGGCCAAG CCCTGTACCT CACATCGCCC ACTGAGATCC AAAGAATCAC CTATAGCCAG
    GAAACCTGTG AAAACCTGCA GGAGATGCTT GGAGAGATGT TTACTCTAGT GGAGACACCA
    GAAGCTCCAA ACAGAGGCTT CTTCAAGGGC TTGTTTGGAG GCGGCGCTCA GTCCTTGGAC
    AGAGAGGATC TGTTTGGAGA GGTGGCTGCT GGGAAGGCCT CACGCAGCCT AGCCCAGCAC
    ATCCCGGGTC CTGGTGGTAT GGAGGGAATG AAGGGGGCGG CGTCAGGTGT GGTGGGAGAG
    CTGGCGCGGG CGAGGATAGC GCTGGATGAG AGAGGACAGA AACTGGGAGA GCTGGAGGAG
    AGGACGGCAG CCATGATGGC CAGCGCAGAC TCCTTCTCCA AACATGCGCA TGATATGATG
    CTGAAATACA AGGACAAGAA ATGGTACCAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46213
    Clone ID Related Accession (Same CDS sequence) XM_009292824.3 , XM_009292824.2
    Accession Version XM_009292824.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product syntaxin-binding protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) and transcript sequence (GFIL01021662.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292824.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 214 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGACACT CTGCATAAGA AGTCTTGCAT GTCCGGCCTC
    TCTAACTTTT ATTCTGAATC AGTGAAAAAG CTTCGTTCTT CTTACCTCAG TCTTTGTGAT
    GGTAATGATG CATCAGCTGC CCCAGAAGAA AGATGCAAAT CACCAACCTC AGATGCCAGA
    GACAACTCAT TCAGCCGCTC GCGCAGCTCC AGTGTGACCA GCATTGACAG AGAGTCACGG
    GAGGTCATTT CCTCCTTTTA CTTTTGCGAC TCTCTGTCCA AGAAGAGCGA GACGCTGGCA
    GTACCCAGTC TGTGGGTGGG CACCTCACTG GGCAGCATGT TGGCCATCGC CCTCACCGTT
    CCCTCCACAC CAGAGCAGAG GATGCAACAG CCTGTGGGCG TTTCTTTCTG TGGTTCAGTT
    GTGCGCTTGA AGGGAGCTAT TCTGCGTATG GCCCAGCTGG ATGCCAGCGG GACTGTCCTT
    CCTCATATTT ATGAGCCCTG GTATGAGCCC AACGCCCCTG AAGAGGAGAG AGAGCGGCCA
    CGGAGACGCA GACCCACGTC CCCTCCGTCA TCTCAGGAGA ACCAGGACTG TCAGTACGCC
    GTGGTCTGCT CTGAGAAACA GGCCAAGGTG GTGGCGCTGC CCTCCCAACA CTGCATCTTT
    GAACACAACA TCACTGAAGC ATCCTTTGTG CTTAGAGCCG ATGTGGTTAC GATGACGGCC
    GGGGTTGCCA TCGCCTGTTT CTGTGCCAAC GGCCACATCA TGACCCTCAG TTTGCCCAGC
    CTGCGGCCAT TGCTGGATGT GAACTACCTT CCTCTGACGG ACATGAGGAT AGCCAGAACC
    TTCTGTTTCT CAAACCAAGG CCAAGCCCTG TACCTCACAT CGCCCACTGA GATCCAAAGA
    ATCACCTATA GCCAGGAAAC CTGTGAAAAC CTGCAGGAGA TGCTTGGAGA GATGTTTACT
    CTAGTGGAGA CACCAGAAGC TCCAAACAGA GGCTTCTTCA AGGGCTTGTT TGGAGGCGGC
    GCTCAGTCCT TGGACAGAGA GGATCTGTTT GGAGAGGTGG CTGCTGGGAA GGCCTCACGC
    AGCCTAGCCC AGCACATCCC GGGTCCTGGT GGTATGGAGG GAATGAAGGG GGCGGCGTCA
    GGTGTGGTGG GAGAGCTGGC GCGGGCGAGG ATAGCGCTGG ATGAGAGAGG ACAGAAACTG
    GGAGAGCTGG AGGAGAGGAC GGCAGCCATG ATGGCCAGCG CAGACTCCTT CTCCAAACAT
    GCGCATGATA TGATGCTGAA ATACAAGGAC AAGAAATGGT ACCAGCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291099.1
    CDS320..3730
    Translation

    Target ORF information:

    RefSeq Version XM_009292824.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292824.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGACACT CTGCATAAGA AGTCTTGCAT GTCCGGCCTC
    TCTAACTTTT ATTCTGAATC AGTGAAAAAG CTTCGTTCTT CTTACCTCAG TCTTTGTGAT
    GGTAATGATG CATCAGCTGC CCCAGAAGAA AGATGCAAAT CACCAACCTC AGATGCCAGA
    GACAACTCAT TCAGCCGCTC GCGCAGCTCC AGTGTGACCA GCATTGACAG AGAGTCACGG
    GAGGTCATTT CCTCCTTTTA CTTTTGCGAC TCTCTGTCCA AGAAGAGCGA GACGCTGGCA
    GTACCCAGTC TGTGGGTGGG CACCTCACTG GGCAGCATGT TGGCCATCGC CCTCACCGTT
    CCCTCCACAC CAGAGCAGAG GATGCAACAG CCTGTGGGCG TTTCTTTCTG TGGTTCAGTT
    GTGCGCTTGA AGGGAGCTAT TCTGCGTATG GCCCAGCTGG ATGCCAGCGG GACTGTCCTT
    CCTCATATTT ATGAGCCCTG GTATGAGCCC AACGCCCCTG AAGAGGAGAG AGAGCGGCCA
    CGGAGACGCA GACCCACGTC CCCTCCGTCA TCTCAGGAGA ACCAGGACTG TCAGTACGCC
    GTGGTCTGCT CTGAGAAACA GGCCAAGGTG GTGGCGCTGC CCTCCCAACA CTGCATCTTT
    GAACACAACA TCACTGAAGC ATCCTTTGTG CTTAGAGCCG ATGTGGTTAC GATGACGGCC
    GGGGTTGCCA TCGCCTGTTT CTGTGCCAAC GGCCACATCA TGACCCTCAG TTTGCCCAGC
    CTGCGGCCAT TGCTGGATGT GAACTACCTT CCTCTGACGG ACATGAGGAT AGCCAGAACC
    TTCTGTTTCT CAAACCAAGG CCAAGCCCTG TACCTCACAT CGCCCACTGA GATCCAAAGA
    ATCACCTATA GCCAGGAAAC CTGTGAAAAC CTGCAGGAGA TGCTTGGAGA GATGTTTACT
    CTAGTGGAGA CACCAGAAGC TCCAAACAGA GGCTTCTTCA AGGGCTTGTT TGGAGGCGGC
    GCTCAGTCCT TGGACAGAGA GGATCTGTTT GGAGAGGTGG CTGCTGGGAA GGCCTCACGC
    AGCCTAGCCC AGCACATCCC GGGTCCTGGT GGTATGGAGG GAATGAAGGG GGCGGCGTCA
    GGTGTGGTGG GAGAGCTGGC GCGGGCGAGG ATAGCGCTGG ATGAGAGAGG ACAGAAACTG
    GGAGAGCTGG AGGAGAGGAC GGCAGCCATG ATGGCCAGCG CAGACTCCTT CTCCAAACAT
    GCGCATGATA TGATGCTGAA ATACAAGGAC AAGAAATGGT ACCAGCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46213
    Clone ID Related Accession (Same CDS sequence) XM_009292824.3 , XM_009292824.2
    Accession Version XM_009292824.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product syntaxin-binding protein 5 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292824.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGACACT CTGCATAAGA AGTCTTGCAT GTCCGGCCTC
    TCTAACTTTT ATTCTGAATC AGTGAAAAAG CTTCGTTCTT CTTACCTCAG TCTTTGTGAT
    GGTAATGATG CATCAGCTGC CCCAGAAGAA AGATGCAAAT CACCAACCTC AGATGCCAGA
    GACAACTCAT TCAGCCGCTC GCGCAGCTCC AGTGTGACCA GCATTGACAG AGAGTCACGG
    GAGGTCATTT CCTCCTTTTA CTTTTGCGAC TCTCTGTCCA AGAAGAGCGA GACGCTGGCA
    GTACCCAGTC TGTGGGTGGG CACCTCACTG GGCAGCATGT TGGCCATCGC CCTCACCGTT
    CCCTCCACAC CAGAGCAGAG GATGCAACAG CCTGTGGGCG TTTCTTTCTG TGGTTCAGTT
    GTGCGCTTGA AGGGAGCTAT TCTGCGTATG GCCCAGCTGG ATGCCAGCGG GACTGTCCTT
    CCTCATATTT ATGAGCCCTG GTATGAGCCC AACGCCCCTG AAGAGGAGAG AGAGCGGCCA
    CGGAGACGCA GACCCACGTC CCCTCCGTCA TCTCAGGAGA ACCAGGACTG TCAGTACGCC
    GTGGTCTGCT CTGAGAAACA GGCCAAGGTG GTGGCGCTGC CCTCCCAACA CTGCATCTTT
    GAACACAACA TCACTGAAGC ATCCTTTGTG CTTAGAGCCG ATGTGGTTAC GATGACGGCC
    GGGGTTGCCA TCGCCTGTTT CTGTGCCAAC GGCCACATCA TGACCCTCAG TTTGCCCAGC
    CTGCGGCCAT TGCTGGATGT GAACTACCTT CCTCTGACGG ACATGAGGAT AGCCAGAACC
    TTCTGTTTCT CAAACCAAGG CCAAGCCCTG TACCTCACAT CGCCCACTGA GATCCAAAGA
    ATCACCTATA GCCAGGAAAC CTGTGAAAAC CTGCAGGAGA TGCTTGGAGA GATGTTTACT
    CTAGTGGAGA CACCAGAAGC TCCAAACAGA GGCTTCTTCA AGGGCTTGTT TGGAGGCGGC
    GCTCAGTCCT TGGACAGAGA GGATCTGTTT GGAGAGGTGG CTGCTGGGAA GGCCTCACGC
    AGCCTAGCCC AGCACATCCC GGGTCCTGGT GGTATGGAGG GAATGAAGGG GGCGGCGTCA
    GGTGTGGTGG GAGAGCTGGC GCGGGCGAGG ATAGCGCTGG ATGAGAGAGG ACAGAAACTG
    GGAGAGCTGG AGGAGAGGAC GGCAGCCATG ATGGCCAGCG CAGACTCCTT CTCCAAACAT
    GCGCATGATA TGATGCTGAA ATACAAGGAC AAGAAATGGT ACCAGCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291099.1
    CDS276..3686
    Translation

    Target ORF information:

    RefSeq Version XM_009292824.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292824.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGACACT CTGCATAAGA AGTCTTGCAT GTCCGGCCTC
    TCTAACTTTT ATTCTGAATC AGTGAAAAAG CTTCGTTCTT CTTACCTCAG TCTTTGTGAT
    GGTAATGATG CATCAGCTGC CCCAGAAGAA AGATGCAAAT CACCAACCTC AGATGCCAGA
    GACAACTCAT TCAGCCGCTC GCGCAGCTCC AGTGTGACCA GCATTGACAG AGAGTCACGG
    GAGGTCATTT CCTCCTTTTA CTTTTGCGAC TCTCTGTCCA AGAAGAGCGA GACGCTGGCA
    GTACCCAGTC TGTGGGTGGG CACCTCACTG GGCAGCATGT TGGCCATCGC CCTCACCGTT
    CCCTCCACAC CAGAGCAGAG GATGCAACAG CCTGTGGGCG TTTCTTTCTG TGGTTCAGTT
    GTGCGCTTGA AGGGAGCTAT TCTGCGTATG GCCCAGCTGG ATGCCAGCGG GACTGTCCTT
    CCTCATATTT ATGAGCCCTG GTATGAGCCC AACGCCCCTG AAGAGGAGAG AGAGCGGCCA
    CGGAGACGCA GACCCACGTC CCCTCCGTCA TCTCAGGAGA ACCAGGACTG TCAGTACGCC
    GTGGTCTGCT CTGAGAAACA GGCCAAGGTG GTGGCGCTGC CCTCCCAACA CTGCATCTTT
    GAACACAACA TCACTGAAGC ATCCTTTGTG CTTAGAGCCG ATGTGGTTAC GATGACGGCC
    GGGGTTGCCA TCGCCTGTTT CTGTGCCAAC GGCCACATCA TGACCCTCAG TTTGCCCAGC
    CTGCGGCCAT TGCTGGATGT GAACTACCTT CCTCTGACGG ACATGAGGAT AGCCAGAACC
    TTCTGTTTCT CAAACCAAGG CCAAGCCCTG TACCTCACAT CGCCCACTGA GATCCAAAGA
    ATCACCTATA GCCAGGAAAC CTGTGAAAAC CTGCAGGAGA TGCTTGGAGA GATGTTTACT
    CTAGTGGAGA CACCAGAAGC TCCAAACAGA GGCTTCTTCA AGGGCTTGTT TGGAGGCGGC
    GCTCAGTCCT TGGACAGAGA GGATCTGTTT GGAGAGGTGG CTGCTGGGAA GGCCTCACGC
    AGCCTAGCCC AGCACATCCC GGGTCCTGGT GGTATGGAGG GAATGAAGGG GGCGGCGTCA
    GGTGTGGTGG GAGAGCTGGC GCGGGCGAGG ATAGCGCTGG ATGAGAGAGG ACAGAAACTG
    GGAGAGCTGG AGGAGAGGAC GGCAGCCATG ATGGCCAGCG CAGACTCCTT CTCCAAACAT
    GCGCATGATA TGATGCTGAA ATACAAGGAC AAGAAATGGT ACCAGCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46214
    Clone ID Related Accession (Same CDS sequence) XM_009292825.2 , XM_009292825.3
    Accession Version XM_009292825.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product syntaxin-binding protein 5 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292825.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGGTCTT TGTGATGGTA ATGATGCATC AGCTGCCCCA
    GAAGAAAGAT GCAAATCACC AACCTCAGCT AAGATATCCC GGAAAATTAA CATGCTTGCT
    GATCAGAAAG ATGATGCCAG AGACAACTCA TTCAGCCGCT CGCGCAGCTC CAGTGTGACC
    AGCATTGACA GAGAGTCACG GGAGGTCATT TCCTCCTTTT ACTTTTGCGA CTCTCTGTCC
    AAGAAGAGCG AGACGCTGGC AGTACCCAGT CTGTGGGTGG GCACCTCACT GGGCAGCATG
    TTGGCCATCG CCCTCACCGT TCCCTCCACA CCAGAGCAGA GGATGCAACA GCCTGTGGGC
    GTTTCTTTCT GTGGTTCAGT TGTGCGCTTG AAGGGAGCTA TTCTGCGTAT GGCCCAGCTG
    GATGCCAGCG GGACTGTCCT TCCTCATATT TATGAGCCCT GGTATGAGCC CAACGCCCCT
    GAAGAGGAGA GAGAGCGGCC ACGGAGACGC AGACCCACGT CCCCTCCGTC ATCTCAGGAG
    AACCAGGACT GTCAGTACGC CGTGGTCTGC TCTGAGAAAC AGGCCAAGGT GGTGGCGCTG
    CCCTCCCAAC ACTGCATCTT TGAACACAAC ATCACTGAAG CATCCTTTGT GCTTAGAGCC
    GATGTGGTTA CGATGACGGC CGGGGTTGCC ATCGCCTGTT TCTGTGCCAA CGGCCACATC
    ATGACCCTCA GTTTGCCCAG CCTGCGGCCA TTGCTGGATG TGAACTACCT TCCTCTGACG
    GACATGAGGA TAGCCAGAAC CTTCTGTTTC TCAAACCAAG GCCAAGCCCT GTACCTCACA
    TCGCCCACTG AGATCCAAAG AATCACCTAT AGCCAGGAAA CCTGTGAAAA CCTGCAGGAG
    ATGCTTGGAG AGATGTTTAC TCTAGTGGAG ACACCAGAAG CTCCAAACAG AGGCTTCTTC
    AAGGGCTTGT TTGGAGGCGG CGCTCAGTCC TTGGACAGAG AGGATCTGTT TGGAGAGGTG
    GCTGCTGGGA AGGCCTCACG CAGCCTAGCC CAGCACATCC CGGGTCCTGG TGGTATGGAG
    GGAATGAAGG GGGCGGCGTC AGGTGTGGTG GGAGAGCTGG CGCGGGCGAG GATAGCGCTG
    GATGAGAGAG GACAGAAACT GGGAGAGCTG GAGGAGAGGA CGGCAGCCAT GATGGCCAGC
    GCAGACTCCT TCTCCAAACA TGCGCATGAT ATGATGCTGA AATACAAGGA CAAGAAATGG
    TACCAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291100.1
    CDS276..3647
    Translation

    Target ORF information:

    RefSeq Version XM_009292825.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292825.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGGTCTT TGTGATGGTA ATGATGCATC AGCTGCCCCA
    GAAGAAAGAT GCAAATCACC AACCTCAGCT AAGATATCCC GGAAAATTAA CATGCTTGCT
    GATCAGAAAG ATGATGCCAG AGACAACTCA TTCAGCCGCT CGCGCAGCTC CAGTGTGACC
    AGCATTGACA GAGAGTCACG GGAGGTCATT TCCTCCTTTT ACTTTTGCGA CTCTCTGTCC
    AAGAAGAGCG AGACGCTGGC AGTACCCAGT CTGTGGGTGG GCACCTCACT GGGCAGCATG
    TTGGCCATCG CCCTCACCGT TCCCTCCACA CCAGAGCAGA GGATGCAACA GCCTGTGGGC
    GTTTCTTTCT GTGGTTCAGT TGTGCGCTTG AAGGGAGCTA TTCTGCGTAT GGCCCAGCTG
    GATGCCAGCG GGACTGTCCT TCCTCATATT TATGAGCCCT GGTATGAGCC CAACGCCCCT
    GAAGAGGAGA GAGAGCGGCC ACGGAGACGC AGACCCACGT CCCCTCCGTC ATCTCAGGAG
    AACCAGGACT GTCAGTACGC CGTGGTCTGC TCTGAGAAAC AGGCCAAGGT GGTGGCGCTG
    CCCTCCCAAC ACTGCATCTT TGAACACAAC ATCACTGAAG CATCCTTTGT GCTTAGAGCC
    GATGTGGTTA CGATGACGGC CGGGGTTGCC ATCGCCTGTT TCTGTGCCAA CGGCCACATC
    ATGACCCTCA GTTTGCCCAG CCTGCGGCCA TTGCTGGATG TGAACTACCT TCCTCTGACG
    GACATGAGGA TAGCCAGAAC CTTCTGTTTC TCAAACCAAG GCCAAGCCCT GTACCTCACA
    TCGCCCACTG AGATCCAAAG AATCACCTAT AGCCAGGAAA CCTGTGAAAA CCTGCAGGAG
    ATGCTTGGAG AGATGTTTAC TCTAGTGGAG ACACCAGAAG CTCCAAACAG AGGCTTCTTC
    AAGGGCTTGT TTGGAGGCGG CGCTCAGTCC TTGGACAGAG AGGATCTGTT TGGAGAGGTG
    GCTGCTGGGA AGGCCTCACG CAGCCTAGCC CAGCACATCC CGGGTCCTGG TGGTATGGAG
    GGAATGAAGG GGGCGGCGTC AGGTGTGGTG GGAGAGCTGG CGCGGGCGAG GATAGCGCTG
    GATGAGAGAG GACAGAAACT GGGAGAGCTG GAGGAGAGGA CGGCAGCCAT GATGGCCAGC
    GCAGACTCCT TCTCCAAACA TGCGCATGAT ATGATGCTGA AATACAAGGA CAAGAAATGG
    TACCAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46214
    Clone ID Related Accession (Same CDS sequence) XM_009292825.2 , XM_009292825.3
    Accession Version XM_009292825.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product syntaxin-binding protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) and transcript sequence (GFIL01021662.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292825.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 214 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGGTCTT TGTGATGGTA ATGATGCATC AGCTGCCCCA
    GAAGAAAGAT GCAAATCACC AACCTCAGCT AAGATATCCC GGAAAATTAA CATGCTTGCT
    GATCAGAAAG ATGATGCCAG AGACAACTCA TTCAGCCGCT CGCGCAGCTC CAGTGTGACC
    AGCATTGACA GAGAGTCACG GGAGGTCATT TCCTCCTTTT ACTTTTGCGA CTCTCTGTCC
    AAGAAGAGCG AGACGCTGGC AGTACCCAGT CTGTGGGTGG GCACCTCACT GGGCAGCATG
    TTGGCCATCG CCCTCACCGT TCCCTCCACA CCAGAGCAGA GGATGCAACA GCCTGTGGGC
    GTTTCTTTCT GTGGTTCAGT TGTGCGCTTG AAGGGAGCTA TTCTGCGTAT GGCCCAGCTG
    GATGCCAGCG GGACTGTCCT TCCTCATATT TATGAGCCCT GGTATGAGCC CAACGCCCCT
    GAAGAGGAGA GAGAGCGGCC ACGGAGACGC AGACCCACGT CCCCTCCGTC ATCTCAGGAG
    AACCAGGACT GTCAGTACGC CGTGGTCTGC TCTGAGAAAC AGGCCAAGGT GGTGGCGCTG
    CCCTCCCAAC ACTGCATCTT TGAACACAAC ATCACTGAAG CATCCTTTGT GCTTAGAGCC
    GATGTGGTTA CGATGACGGC CGGGGTTGCC ATCGCCTGTT TCTGTGCCAA CGGCCACATC
    ATGACCCTCA GTTTGCCCAG CCTGCGGCCA TTGCTGGATG TGAACTACCT TCCTCTGACG
    GACATGAGGA TAGCCAGAAC CTTCTGTTTC TCAAACCAAG GCCAAGCCCT GTACCTCACA
    TCGCCCACTG AGATCCAAAG AATCACCTAT AGCCAGGAAA CCTGTGAAAA CCTGCAGGAG
    ATGCTTGGAG AGATGTTTAC TCTAGTGGAG ACACCAGAAG CTCCAAACAG AGGCTTCTTC
    AAGGGCTTGT TTGGAGGCGG CGCTCAGTCC TTGGACAGAG AGGATCTGTT TGGAGAGGTG
    GCTGCTGGGA AGGCCTCACG CAGCCTAGCC CAGCACATCC CGGGTCCTGG TGGTATGGAG
    GGAATGAAGG GGGCGGCGTC AGGTGTGGTG GGAGAGCTGG CGCGGGCGAG GATAGCGCTG
    GATGAGAGAG GACAGAAACT GGGAGAGCTG GAGGAGAGGA CGGCAGCCAT GATGGCCAGC
    GCAGACTCCT TCTCCAAACA TGCGCATGAT ATGATGCTGA AATACAAGGA CAAGAAATGG
    TACCAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291100.1
    CDS319..3690
    Translation

    Target ORF information:

    RefSeq Version XM_009292825.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio syntaxin binding protein 5b (tomosyn) (stxbp5b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292825.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCACATCT CTGCGTTCGT TTCGCATTCG CTGGAAAACA ACATGAAGAA GTTTAATATC 
    AGGAAGGTGT TGGACGGATT GACCGCGGCG TCTTCTTCCT CCTCCTCTGC CGCCGCCGCG
    TCCCCTCAGT CGGGGAATGA CATGATTCAG GAGACCCTGC AGTCCGAGCA TTTCCAGCTC
    TGCAAGACAG TGCGGCATGG CTTTCCCTAT CAACCCTCCT CCATGGCTTT CGACCCTGTG
    CAGAAAATCC TGGCTATTGG CACACAGAGT GGAGCCCTGA GACTATTTGG CCGAGCAGGA
    GTTGAGTGTT ACTGTCAGCA TGAGAGCGGA TCCGCTGTCA TCCAGCTGGA GTTTCTTGTC
    AATGTAGGGG CGCTGGTTAG TGCTCTGGCA GATGACAGTA TTCATTTGTG GAATTTGAGG
    CAAAAGCTTC CAGCCGTTCT CCATTCTCTT AAATTTAATC GGGAGAGGAT CACGTTTTGC
    CATTTGCCTT TCCAGAGTAA ATGGCTGTAT GTGGGCACGG AAAGAGGGAA CATTCATATC
    GTCAACGTGG AGTCCTTCAC CCTCTCAGGC TATGTGATCA TGTGGAACAA AGCCATTGAA
    CTTTCATCAA AGACTCACCC TGGACCAGTA GTTCACATCA GCGATAACCC CATGGATGAA
    GGAAAACTTT TAATTGGTTT TGAATGTGGG ATAGTTGTGC TGTGGGACTT GAAATCGAAG
    AAGGCAGACT ACCGCTACAA CTATGATGAG GCGATCCACT CTGTGGCTTG GCATCACGAG
    GGGAAACAGT TTGTGTGCAG TCACTCGGAT GGCACACTTA CCACATGGAA TGTGCGCGCT
    CCTGCCAAGC CTGCCCAGAT CATTACACCG CATGGTAAAC AGCCAAAGGA TGGGAAGAAG
    CCGGAGCCTT GCAAACCCAT CTTAAAAGTG GAGTACAAAA CCACCAGAGC AGGGGACCCA
    TTCATGATAC TGTGTGGAGG TTTGTCATAT GATACAGTGG GTAGGCGAGC CTGTCTGACT
    GTGATGCATG GGAAAAGCAC TGCAGTGCTG GAGATGGACT ACCCCATAGT GGATTTCCTT
    ACGCTGTGTG AAACCCCATA TCCAAACGAT TTCCAGGAGC CTTATGCAGT TGTTGTCCTA
    CTGGAGAAGG ATTTGGTTGT TATTGACCTT GCACAAATTG GGTATCCCAT CTTTGAGAAC
    CCGTACCCTC TATCCATCCA CGAGTCTCCG GTGACCTGCT GCGAATATTT TGCAGACTGT
    CCAGCCGAAG CAATCCCTGC ACTTTACTCT GTTGGCTCCA GACAGAAAAG ACAGGGCTTC
    AGTAAGAAGG AATGGCCCAT AAGTGCAGGT AACTGGGGCC AGGGAACACT GAGTTTCTCA
    GAGATTATCA TCACTGGGCA TGCTGATGGA TCGATCAAGT TTTGGGATGC CTCAGCTTTA
    ATGCTTCAAG TGTTGTATAA GCTGAAAACA GCGAAGGTGT TTGACAAGCC CCGGAGTAAA
    GAGGAGAAAT CCAGCACTGA TATTGTGGAT GAGGATCCAT TCGCCATCCA GATGCTGTCC
    TGGTGTCCGG AGAGCCGTGT GCTGTGTGTG GCTGGAGTAT CGGCTCATGT TATAGTCTAC
    AGGTTCAGCA AACAGGAAGT CACCACCGAA GTGGTTCAGC TGTTGGAGGT GCGTATGCAG
    TGTGAGCTGA ATGAAGTGGA ATCTCCAGAG GGTGGAGGAG ACCAGGCGTC GGCTGTTTCC
    ACCCCAGGAA CTGCACCCAG CCCTCAGACC AGCCTGCCTC AGTCTCACCC CTCCACAAGC
    AGCAACAACT CCTGCGACGG CCTTCGGGAT AACGTGCCCT GCCTCAAGGT CAGGAGTTCC
    CCTCTCAAAC AGTTTCCCGG TTATCATGTG GAGCTGCTCA TTCAGCTGGT GTGGGTCAGC
    GGGGAGCCAC CACAGCAGAT CACCAGCCTG GCCATCAACT CTGCGTATGG CCTAGTCGCT
    TTTGGCAATG GAAACGGCCT TGCAGTTGTT GATTACATTC AGAAAACTCT CCTTCTGAAC
    GTGACCACCG CCGAACTGTA CGGCTCTACA GATCCTCACC ACAGACAACC ACGCTCACCC
    CGCAAAACCA GACAGCCCTC AGCAGGTCTT TGTGATGGTA ATGATGCATC AGCTGCCCCA
    GAAGAAAGAT GCAAATCACC AACCTCAGCT AAGATATCCC GGAAAATTAA CATGCTTGCT
    GATCAGAAAG ATGATGCCAG AGACAACTCA TTCAGCCGCT CGCGCAGCTC CAGTGTGACC
    AGCATTGACA GAGAGTCACG GGAGGTCATT TCCTCCTTTT ACTTTTGCGA CTCTCTGTCC
    AAGAAGAGCG AGACGCTGGC AGTACCCAGT CTGTGGGTGG GCACCTCACT GGGCAGCATG
    TTGGCCATCG CCCTCACCGT TCCCTCCACA CCAGAGCAGA GGATGCAACA GCCTGTGGGC
    GTTTCTTTCT GTGGTTCAGT TGTGCGCTTG AAGGGAGCTA TTCTGCGTAT GGCCCAGCTG
    GATGCCAGCG GGACTGTCCT TCCTCATATT TATGAGCCCT GGTATGAGCC CAACGCCCCT
    GAAGAGGAGA GAGAGCGGCC ACGGAGACGC AGACCCACGT CCCCTCCGTC ATCTCAGGAG
    AACCAGGACT GTCAGTACGC CGTGGTCTGC TCTGAGAAAC AGGCCAAGGT GGTGGCGCTG
    CCCTCCCAAC ACTGCATCTT TGAACACAAC ATCACTGAAG CATCCTTTGT GCTTAGAGCC
    GATGTGGTTA CGATGACGGC CGGGGTTGCC ATCGCCTGTT TCTGTGCCAA CGGCCACATC
    ATGACCCTCA GTTTGCCCAG CCTGCGGCCA TTGCTGGATG TGAACTACCT TCCTCTGACG
    GACATGAGGA TAGCCAGAAC CTTCTGTTTC TCAAACCAAG GCCAAGCCCT GTACCTCACA
    TCGCCCACTG AGATCCAAAG AATCACCTAT AGCCAGGAAA CCTGTGAAAA CCTGCAGGAG
    ATGCTTGGAG AGATGTTTAC TCTAGTGGAG ACACCAGAAG CTCCAAACAG AGGCTTCTTC
    AAGGGCTTGT TTGGAGGCGG CGCTCAGTCC TTGGACAGAG AGGATCTGTT TGGAGAGGTG
    GCTGCTGGGA AGGCCTCACG CAGCCTAGCC CAGCACATCC CGGGTCCTGG TGGTATGGAG
    GGAATGAAGG GGGCGGCGTC AGGTGTGGTG GGAGAGCTGG CGCGGGCGAG GATAGCGCTG
    GATGAGAGAG GACAGAAACT GGGAGAGCTG GAGGAGAGGA CGGCAGCCAT GATGGCCAGC
    GCAGACTCCT TCTCCAAACA TGCGCATGAT ATGATGCTGA AATACAAGGA CAAGAAATGG
    TACCAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.