appl1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	appl1
Entrez Gene ID	571540
Full Name	adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
General protein information	Preferred Names adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 Names DCC-interacting protein 13-alpha
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001128074.1	NP_001121546.1	DCC-interacting protein 13-alpha
XM_021479657.1	XP_021335332.1	DCC-interacting protein 13-alpha isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	APPL1	XP_001173707.1
Canis lupus familiaris (dog)	APPL1	XP_849075.1
Gallus gallus (chicken)	APPL1	XP_414344.4
Bos taurus (cattle)	APPL1	NP_001092473.1
Mus musculus (house mouse)	Appl1	NP_660256.1
Homo sapiens (human)	APPL1	NP_036228.1
Rattus norvegicus (Norway rat)	Appl1	XP_006252792.1
Danio rerio (zebrafish)	appl1	NP_001121546.1
Xenopus tropicalis (tropical clawed frog)	appl1	XP_004914231.1

Gene Ontology
Bibliography

Related articles in PubMed

Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish.
Kok FO, Shin M, Ni CW, Gupta A, Grosse AS, van Impel A, Kirchmaier BC, Peterson-Maduro J, Kourkoulis G, Male I, DeSantis DF, Sheppard-Tindell S, Ebarasi L, Betsholtz C, Schulte-Merker S, Wolfe SA, Lawson ND
Developmental cell32(1)97-108(2015 Jan)

Altering PI3K-Akt signalling in zebrafish embryos affects PTEN phosphorylation and gastrulation.
Finkielsztein A, Kelly GM
Biology of the cell101(11)661-78, 4 p following 678(2009 Aug)

The endosomal protein Appl1 mediates Akt substrate specificity and cell survival in vertebrate development.
Schenck A, Goto-Silva L, Collinet C, Rhinn M, Giner A, Habermann B, Brand M, Zerial M
Cell133(3)486-97(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study reports that Appl1 regulates the activity of Akt and, importantly, its downstream signaling specificity from an endosomal compartment, with profound implications for development.
Title: The endosomal protein Appl1 mediates Akt substrate specificity and cell survival in vertebrate development.

The following appl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the appl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa04720	NM_001128074.1 Latest version!	Danio rerio adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (appl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa147909	XM_021479657.1 Latest version!	Danio rerio adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (appl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

appl1 ( NM_001128074.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001121546.1 Danio rerio adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (appl1), mRNA.

Price & Availability↑

CloneID	ODa04720
Clone ID Related Accession (Same CDS sequence)	NM_001128074.1
Accession Version	NM_001128074.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-24
Organism	Danio rerio(zebrafish)
Product	DCC-interacting protein 13-alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU053152.1. On Jul 31, 2008 this sequence version replaced XR_029597.2. ##Evidence-Data-START## Transcript exon combination :: EU053152.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCCTGGAA TCGATAAACT ACCGATAGAG GAAACTCTGG AGGACAGTCC GCAGACTCGT 
TCTCTGCTTG GTGTGTTTGA AGAAGATGCA GCAGCGATTT CCAGTTATTT CAGTCAGCTT 
TTCAAAGCCA TGCAGAGGAT CTACGATGCC CAGAATGAAC TGAGCGCTGC CACACATCTG 
ACATCGAAAC TACTGAAAGA CTACGAGAAG CAGCGTTTTC CTCTAGGTGG CGACGACGAG 
GTGATGAGCT CCACTTTGCA GCAGTTTGCT AAAGTTATCG ACGAGCTCAG CTCCTGTCAC 
GCGGTTCTCT CCACGCAACT CGCAGACGCC ATGATGTTCC CCATCACACA GTTTCAGGAG 
AGAGACCTGA GAGAAATTCT CATCCTGAAG GAAGTGTTTC AGCTTGCAAG TGATGATCAC 
GATGCTGCCG TACACAGATA CAGTCGTCTG TCGAAGCGGA AGGAGAATGA GAAGGTGAAG 
AATGAAGTGA TGGAGGATGT TTACACGTCC AGAAAGAAGC AGCACGAGAC CACAATGCAC 
TACTTTGCGT CTCTCAATAT GCTGCAATAT AAGAAGAAGA TCGCTCTGCT GGAGCCGCTA 
CTGGGATATA TGCAGGCGCA GATCAGTTTC TTCAAGCTGG GATCTGAGAA TCTGACGCAG 
CAGTGGGAGG ATTTCCTTAC TAATATTGGC ACCAGCGTGC AGAACGTTCG CAGGGAAATG 
GACAGCGATG CGGAAGCGAT GGAGCAGACG ATTCAGGACT TGAAAGAAGC CAGTGACCCA 
CTCTACATGC CCGACCCAGA CCCCAACAGG ATCCCTGTCA ACCGCAATCT GACACGCAAA 
GCCGGATACC TCAACACTCG CAATAAAACG GGCCTGGTGT CGTCCAGCTG GGAGCGTCAG 
TACTTCTTCA CGCAGGGAGG AAACCTGATG AGCCAGAGCA GAGGAGCCGT GGCTGGAGGA 
CTCGTCATGG ACATCGACAA CTGCTCCGTC ATGGCCGTGG ACTGTGAGGA CCGGCGCTTC 
TGCTTTCAGA TCACTGCCTT CGATGGAAAG AAAGCTGCAG TATTACAGGC GGACAGCAGG 
AAAGACTGTG AGGAGTGGAT TGCAACTATA AACAACATCT CCAAGAGGAT ATACCTCAGT 
GAAAACCCAG AGGAGATCGC AGCCAGAGTG AATCAATCCG CACTGGAGGC CGTGACTCCT 
TCACCGTCTT TCCAGCAGAG ACACGAGAGC TTCAGACCAA ACACGCAGGG TAAAGTGAAA 
GCGTCCCGTT CAGGCAGTCA GTGCTCTGTG GGCTCCGAGT CTCCAGCTCT GTCGTCTCTG 
TCTCTGGATT CACTGGTTGC TCCTGATACT CCCATACAGT TCGACATCAT TTCCCCCGTC 
AGCGAGGAGC ACACAAGTCA GACGAAAAGC AGTGCACAGG GCAGGACAAA CCCATTCGGG 
GAATCTGGAG GATCACAATC TGAAGAAAGT GAAGACTCGA TCCTGCATCA GTTGTTCATC 
GTGCGCTTTC TGGGCTCTAT GGAGGTGAAG GCCACAGAGA GCTCAGACGT CATTTATGAG 
ACCATGAGGC AGATCCTGGC CGCTCGAGCC ATTCATAACA TCTTCAGGAT GACCGAATCA 
CACCTGCTGG TCACCTGCGA ATGTCTGAAG CTCATTGACC CACAGACGCA AGTCACCAGA 
TTACGGTTTC CGCTCTCCAG TGTGGTTCTG TGTGCGTCGC ATCAGGAGAA CAAGCGTTTG 
TTTGGTTTTG TGCTGCAGAC AGCAGGAGGA CGAGCAGACG GACAGCCTGT TACCGTCTGC 
TACATCTTTG AGTCAAACAA TGATGGAGAG AAGATCTGTG ACAGTATAGG CCTGGCCAAG 
CAGATCGCCC TCCACTCTGA GATGGACCGC AAAGCCTCAC AGAAACAGAG AGAGCTGGAC 
AAAGCCAAGG AGAAACAGCA GGAAGAACTC CATAAACAAA AACAAATCGA GAAGGATCTG 
GAGGAACAGA GTCGTCTGAT TGCCGCCTCT AGTCGACCAA ACCAACCAGC AGCTGACGGA 
CAGTTCTTGG TCCTAAGCAA CAGCCAATCA GAGGACAGCG ACGTAGGAGA GGAGGGGCTG 
AAAAAGGGTG AATCAGAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121546.1
CDS	43..2166
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDAAAISSYFSQLFKAMQRIYDAQNELSAATHLTSKLLKDYEKQRFPLGGDDEVMSSTLQQFAKVIDELSSCHAVLSTQLADAMMFPITQFQERDLREILILKEVFQLASDDHDAAVHRYSRLSKRKENEKVKNEVMEDVYTSRKKQHETTMHYFASLNMLQYKKKIALLEPLLGYMQAQISFFKLGSENLTQQWEDFLTNIGTSVQNVRREMDSDAEAMEQTIQDLKEASDPLYMPDPDPNRIPVNRNLTRKAGYLNTRNKTGLVSSSWERQYFFTQGGNLMSQSRGAVAGGLVMDIDNCSVMAVDCEDRRFCFQITAFDGKKAAVLQADSRKDCEEWIATINNISKRIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESFRPNTQGKVKASRSGSQCSVGSESPALSSLSLDSLVAPDTPIQFDIISPVSEEHTSQTKSSAQGRTNPFGESGGSQSEESEDSILHQLFIVRFLGSMEVKATESSDVIYETMRQILAARAIHNIFRMTESHLLVTCECLKLIDPQTQVTRLRFPLSSVVLCASHQENKRLFGFVLQTAGGRADGQPVTVCYIFESNNDGEKICDSIGLAKQIALHSEMDRKASQKQRELDKAKEKQQEELHKQKQIEKDLEEQSRLIAASSRPNQPAADGQFLVLSNSQSEDSDVGEEGLKKGESEA

Target ORF information:

RefSeq Version	NM_001128074.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (appl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128074.1

ORF Insert Sequence:

ATGCCTGGAA TCGATAAACT ACCGATAGAG GAAACTCTGG AGGACAGTCC GCAGACTCGT 
TCTCTGCTTG GTGTGTTTGA AGAAGATGCA GCAGCGATTT CCAGTTATTT CAGTCAGCTT 
TTCAAAGCCA TGCAGAGGAT CTACGATGCC CAGAATGAAC TGAGCGCTGC CACACATCTG 
ACATCGAAAC TACTGAAAGA CTACGAGAAG CAGCGTTTTC CTCTAGGTGG CGACGACGAG 
GTGATGAGCT CCACTTTGCA GCAGTTTGCT AAAGTTATCG ACGAGCTCAG CTCCTGTCAC 
GCGGTTCTCT CCACGCAACT CGCAGACGCC ATGATGTTCC CCATCACACA GTTTCAGGAG 
AGAGACCTGA GAGAAATTCT CATCCTGAAG GAAGTGTTTC AGCTTGCAAG TGATGATCAC 
GATGCTGCCG TACACAGATA CAGTCGTCTG TCGAAGCGGA AGGAGAATGA GAAGGTGAAG 
AATGAAGTGA TGGAGGATGT TTACACGTCC AGAAAGAAGC AGCACGAGAC CACAATGCAC 
TACTTTGCGT CTCTCAATAT GCTGCAATAT AAGAAGAAGA TCGCTCTGCT GGAGCCGCTA 
CTGGGATATA TGCAGGCGCA GATCAGTTTC TTCAAGCTGG GATCTGAGAA TCTGACGCAG 
CAGTGGGAGG ATTTCCTTAC TAATATTGGC ACCAGCGTGC AGAACGTTCG CAGGGAAATG 
GACAGCGATG CGGAAGCGAT GGAGCAGACG ATTCAGGACT TGAAAGAAGC CAGTGACCCA 
CTCTACATGC CCGACCCAGA CCCCAACAGG ATCCCTGTCA ACCGCAATCT GACACGCAAA 
GCCGGATACC TCAACACTCG CAATAAAACG GGCCTGGTGT CGTCCAGCTG GGAGCGTCAG 
TACTTCTTCA CGCAGGGAGG AAACCTGATG AGCCAGAGCA GAGGAGCCGT GGCTGGAGGA 
CTCGTCATGG ACATCGACAA CTGCTCCGTC ATGGCCGTGG ACTGTGAGGA CCGGCGCTTC 
TGCTTTCAGA TCACTGCCTT CGATGGAAAG AAAGCTGCAG TATTACAGGC GGACAGCAGG 
AAAGACTGTG AGGAGTGGAT TGCAACTATA AACAACATCT CCAAGAGGAT ATACCTCAGT 
GAAAACCCAG AGGAGATCGC AGCCAGAGTG AATCAATCCG CACTGGAGGC CGTGACTCCT 
TCACCGTCTT TCCAGCAGAG ACACGAGAGC TTCAGACCAA ACACGCAGGG TAAAGTGAAA 
GCGTCCCGTT CAGGCAGTCA GTGCTCTGTG GGCTCCGAGT CTCCAGCTCT GTCGTCTCTG 
TCTCTGGATT CACTGGTTGC TCCTGATACT CCCATACAGT TCGACATCAT TTCCCCCGTC 
AGCGAGGAGC ACACAAGTCA GACGAAAAGC AGTGCACAGG GCAGGACAAA CCCATTCGGG 
GAATCTGGAG GATCACAATC TGAAGAAAGT GAAGACTCGA TCCTGCATCA GTTGTTCATC 
GTGCGCTTTC TGGGCTCTAT GGAGGTGAAG GCCACAGAGA GCTCAGACGT CATTTATGAG 
ACCATGAGGC AGATCCTGGC CGCTCGAGCC ATTCATAACA TCTTCAGGAT GACCGAATCA 
CACCTGCTGG TCACCTGCGA ATGTCTGAAG CTCATTGACC CACAGACGCA AGTCACCAGA 
TTACGGTTTC CGCTCTCCAG TGTGGTTCTG TGTGCGTCGC ATCAGGAGAA CAAGCGTTTG 
TTTGGTTTTG TGCTGCAGAC AGCAGGAGGA CGAGCAGACG GACAGCCTGT TACCGTCTGC 
TACATCTTTG AGTCAAACAA TGATGGAGAG AAGATCTGTG ACAGTATAGG CCTGGCCAAG 
CAGATCGCCC TCCACTCTGA GATGGACCGC AAAGCCTCAC AGAAACAGAG AGAGCTGGAC 
AAAGCCAAGG AGAAACAGCA GGAAGAACTC CATAAACAAA AACAAATCGA GAAGGATCTG 
GAGGAACAGA GTCGTCTGAT TGCCGCCTCT AGTCGACCAA ACCAACCAGC AGCTGACGGA 
CAGTTCTTGG TCCTAAGCAA CAGCCAATCA GAGGACAGCG ACGTAGGAGA GGAGGGGCTG 
AAAAAGGGTG AATCAGAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

appl1 ( XM_021479657.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335332.1 Danio rerio adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (appl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa147909
Clone ID Related Accession (Same CDS sequence)	XM_021479657.1
Accession Version	XM_021479657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DCC-interacting protein 13-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAA TCGATAAACT ACCGATAGAG GAAACTCTGG AGGACAGTCC GCAGACTCGT 
TCTCTGCTGG GTGTGTTTGA AGAAGATGCA GCAGCGATTT CCAGTTATTT CAATCAGCTT 
TTCAAAGCCA TGCAGAGGAT CTACGACGCC CAGAATGAAC TGAGCGCTGC CACACATCTG 
ACATCGAAAC TACTGAAAGA CTACGAGAAG CAGCGTTTTC CTTTAGGTGG CGACGACGAG 
GTGATGAGCT CCACTTTGCA GCAGTTTGCT AAAGTTATCG ACGAGCTCAG CTCCTGTCAC 
GCGGTTCTCT CCACGCAACT CGCAGATGCC ATGATGTTCC CCATCACACA GTTTCAGGAG 
AGAGACCTGA GAGAAATTCT CATCCTGAAG GAAGTGTTTC AGCTCGCAAG TGATGATCAC 
GATGCTGCCG TACACAGATA CAGTCGTCTG TCGAAGCGGA AGGAGAATGA GAAGGTGAAG 
AATGAAGTGA TGGAGGATGT TTACACGTCC AGAAAGAAGC AGCACGAGAC CACAATGCAC 
TACTTTGCGT CTCTCAATAT GCTGCAATAT AAGAAGAAGA TCGCTCTGCT GGAGCCGCTA 
CTGGGATATA TGCAGGCGCA GATCAGTTTC TTCAAACTGG GATCTGAGAA TCTGACGCAG 
CAGTGGGAGG ATTTCCTTAC TAATATTGGC ACCAGCGTGC AGAACGTTCG CAGGGAAATG 
GACAGCGATG CGGAAGCGAT GGAGCAGACG ATTCAGGACT TGAAAGAAGC CAGTGACCCA 
CTCTACATGC CCGACCCAGA CCCCAACAGG ATCCCTGTCA ACCGCAATCT GACACGCAAA 
GCCGGATACC TCAACACTCG CAATAAAACG GGCCTGGTGT CGTCCAGCTG GGAGCGTCAG 
TACTTCTTCA CGCAGGGAGG AAACCTGATG AGCCAGAGCA GAGGAGCCGT GGCTGGAGGA 
CTCGTCATGG ACATCGACAA CTGCTCCGTC ATGGCCGTGG ACTGTGAGGA CCGGCGCTTC 
TGCTTTCAGA TCACTGCCTT CGATGGAAAG AAAGCTGCAG TATTACAGGC GGACAGCAGG 
AAAGACTGTG AGGAGTGGAT TGCAACAATA AACAACATCT CCAAGAGGAT ATACCTCAGT 
GAAAACCCAG AGGAGATCGC AGCCAGAGTG AATCAATCCG CACTGGAGGC AGTGACTCCT 
TCACCGTCTT TCCAGCAGAG ACACGAGAGC TTCAGACCAA ACACGCAGGG TAAAGTGAAA 
GCGTCCCGTT CAGGCAGTCA GTGCTCTGTG GGCTCCGAGT CTCCAGCTCT GTCATCTCTG 
TCTCTGGATT CACTGGTTGC TCCTGATACT CCCATACAAT TCGACATCAT TTCCCCCGTC 
AGCGAGGAGC ACACAAGTCA GACGAAAAGC AGTGCACAGG GCAGAAGGAC AAACCCATTC 
GGGGAATCTG GAGGATCACA ATCTGAAGAA AGTGAAGACT CGATCCTGCA TCAGTTGTTC 
ATCGTGCGCT TTCTGGGCTC TATGGAGGTG AAGGCCACAG AGAGCTCAGA CGTCATTTAT 
GAGACCATGA GGCAGATCCT GGCCGCTCGA GCCATTCATA ACATCTTCAG GATGACCGAA 
TCACACCTGC TGGTCACCTG CGAATGTCTG AAGCTCATTG ACCCACAGAC GCAAGTCACC 
AGATTACGGT TTCCGCTCTC CAGTGTGGTT CTGTGTGCGT CGCATCAGGA GAACAAGCGT 
TTGTTTGGTT TTGTGCTGCA GACAGCAGGA GGACGAGCAG ACGGACAGCC TGTTACCGTC 
TGCTACATCT TTGAGTCAAA CAATGATGGA GAGAAGATCT GTGACAGTAT AGGCCTGGCC 
AAGCAGATCG CCCTCCACTC TGAGATGGAC CGCAAAGCCT CACAGAAACA GAGAGAGCTG 
GACAAAGCCA AGGAGAAACA GCAGGAAGAA CTCCATAAAC AAAAACAAAT CGAGAAGGAT 
CTGGAGGAAC AGAGTCGTCT GATTGCCGCC TCTAGTCGAC CAAACCAACC AGCAGCTGAC 
GGACAGTTCT TGGTCCTAAG CAACAGCCAA TCAGAGGACA GCGACGTAGG AGAGGAGGGG 
CTGAAAAAGG GTGAATCAGA AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335332.1
CDS	98..2224
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDAAAISSYFNQLFKAMQRIYDAQNELSAATHLTSKLLKDYEKQRFPLGGDDEVMSSTLQQFAKVIDELSSCHAVLSTQLADAMMFPITQFQERDLREILILKEVFQLASDDHDAAVHRYSRLSKRKENEKVKNEVMEDVYTSRKKQHETTMHYFASLNMLQYKKKIALLEPLLGYMQAQISFFKLGSENLTQQWEDFLTNIGTSVQNVRREMDSDAEAMEQTIQDLKEASDPLYMPDPDPNRIPVNRNLTRKAGYLNTRNKTGLVSSSWERQYFFTQGGNLMSQSRGAVAGGLVMDIDNCSVMAVDCEDRRFCFQITAFDGKKAAVLQADSRKDCEEWIATINNISKRIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESFRPNTQGKVKASRSGSQCSVGSESPALSSLSLDSLVAPDTPIQFDIISPVSEEHTSQTKSSAQGRRTNPFGESGGSQSEESEDSILHQLFIVRFLGSMEVKATESSDVIYETMRQILAARAIHNIFRMTESHLLVTCECLKLIDPQTQVTRLRFPLSSVVLCASHQENKRLFGFVLQTAGGRADGQPVTVCYIFESNNDGEKICDSIGLAKQIALHSEMDRKASQKQRELDKAKEKQQEELHKQKQIEKDLEEQSRLIAASSRPNQPAADGQFLVLSNSQSEDSDVGEEGLKKGESEA

Target ORF information:

RefSeq Version	XM_021479657.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (appl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479657.1

ORF Insert Sequence:

ATGCCTGGAA TCGATAAACT ACCGATAGAG GAAACTCTGG AGGACAGTCC GCAGACTCGT 
TCTCTGCTGG GTGTGTTTGA AGAAGATGCA GCAGCGATTT CCAGTTATTT CAATCAGCTT 
TTCAAAGCCA TGCAGAGGAT CTACGACGCC CAGAATGAAC TGAGCGCTGC CACACATCTG 
ACATCGAAAC TACTGAAAGA CTACGAGAAG CAGCGTTTTC CTTTAGGTGG CGACGACGAG 
GTGATGAGCT CCACTTTGCA GCAGTTTGCT AAAGTTATCG ACGAGCTCAG CTCCTGTCAC 
GCGGTTCTCT CCACGCAACT CGCAGATGCC ATGATGTTCC CCATCACACA GTTTCAGGAG 
AGAGACCTGA GAGAAATTCT CATCCTGAAG GAAGTGTTTC AGCTCGCAAG TGATGATCAC 
GATGCTGCCG TACACAGATA CAGTCGTCTG TCGAAGCGGA AGGAGAATGA GAAGGTGAAG 
AATGAAGTGA TGGAGGATGT TTACACGTCC AGAAAGAAGC AGCACGAGAC CACAATGCAC 
TACTTTGCGT CTCTCAATAT GCTGCAATAT AAGAAGAAGA TCGCTCTGCT GGAGCCGCTA 
CTGGGATATA TGCAGGCGCA GATCAGTTTC TTCAAACTGG GATCTGAGAA TCTGACGCAG 
CAGTGGGAGG ATTTCCTTAC TAATATTGGC ACCAGCGTGC AGAACGTTCG CAGGGAAATG 
GACAGCGATG CGGAAGCGAT GGAGCAGACG ATTCAGGACT TGAAAGAAGC CAGTGACCCA 
CTCTACATGC CCGACCCAGA CCCCAACAGG ATCCCTGTCA ACCGCAATCT GACACGCAAA 
GCCGGATACC TCAACACTCG CAATAAAACG GGCCTGGTGT CGTCCAGCTG GGAGCGTCAG 
TACTTCTTCA CGCAGGGAGG AAACCTGATG AGCCAGAGCA GAGGAGCCGT GGCTGGAGGA 
CTCGTCATGG ACATCGACAA CTGCTCCGTC ATGGCCGTGG ACTGTGAGGA CCGGCGCTTC 
TGCTTTCAGA TCACTGCCTT CGATGGAAAG AAAGCTGCAG TATTACAGGC GGACAGCAGG 
AAAGACTGTG AGGAGTGGAT TGCAACAATA AACAACATCT CCAAGAGGAT ATACCTCAGT 
GAAAACCCAG AGGAGATCGC AGCCAGAGTG AATCAATCCG CACTGGAGGC AGTGACTCCT 
TCACCGTCTT TCCAGCAGAG ACACGAGAGC TTCAGACCAA ACACGCAGGG TAAAGTGAAA 
GCGTCCCGTT CAGGCAGTCA GTGCTCTGTG GGCTCCGAGT CTCCAGCTCT GTCATCTCTG 
TCTCTGGATT CACTGGTTGC TCCTGATACT CCCATACAAT TCGACATCAT TTCCCCCGTC 
AGCGAGGAGC ACACAAGTCA GACGAAAAGC AGTGCACAGG GCAGAAGGAC AAACCCATTC 
GGGGAATCTG GAGGATCACA ATCTGAAGAA AGTGAAGACT CGATCCTGCA TCAGTTGTTC 
ATCGTGCGCT TTCTGGGCTC TATGGAGGTG AAGGCCACAG AGAGCTCAGA CGTCATTTAT 
GAGACCATGA GGCAGATCCT GGCCGCTCGA GCCATTCATA ACATCTTCAG GATGACCGAA 
TCACACCTGC TGGTCACCTG CGAATGTCTG AAGCTCATTG ACCCACAGAC GCAAGTCACC 
AGATTACGGT TTCCGCTCTC CAGTGTGGTT CTGTGTGCGT CGCATCAGGA GAACAAGCGT 
TTGTTTGGTT TTGTGCTGCA GACAGCAGGA GGACGAGCAG ACGGACAGCC TGTTACCGTC 
TGCTACATCT TTGAGTCAAA CAATGATGGA GAGAAGATCT GTGACAGTAT AGGCCTGGCC 
AAGCAGATCG CCCTCCACTC TGAGATGGAC CGCAAAGCCT CACAGAAACA GAGAGAGCTG 
GACAAAGCCA AGGAGAAACA GCAGGAAGAA CTCCATAAAC AAAAACAAAT CGAGAAGGAT 
CTGGAGGAAC AGAGTCGTCT GATTGCCGCC TCTAGTCGAC CAAACCAACC AGCAGCTGAC 
GGACAGTTCT TGGTCCTAAG CAACAGCCAA TCAGAGGACA GCGACGTAGG AGAGGAGGGG 
CTGAAAAAGG GTGAATCAGA AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish.
Developmental cell32(1)97-108(2015 Jan)
Kok FO,Shin M,Ni CW,Gupta A,Grosse AS,van Impel A,Kirchmaier BC,Peterson-Maduro J,Kourkoulis G,Male I,DeSantis DF,Sheppard-Tindell S,Ebarasi L,Betsholtz C,Schulte-Merker S,Wolfe SA,Lawson ND

Altering PI3K-Akt signalling in zebrafish embryos affects PTEN phosphorylation and gastrulation.
Biology of the cell101(11)661-78, 4 p following 678(2009 Aug)
Finkielsztein A,Kelly GM

The endosomal protein Appl1 mediates Akt substrate specificity and cell survival in vertebrate development.
Cell133(3)486-97(2008 May)
Schenck A,Goto-Silva L,Collinet C,Rhinn M,Giner A,Habermann B,Brand M,Zerial M

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

appl1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報