Appl1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Appl1
Entrez Gene ID	72993
Full Name	adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Synonyms	2900057D21Rik,7330406P05Rik,AI585782,AW209077,BB022931,C88264,DIP13
General protein information	Preferred Names adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 Names DCC-interacting protein 13-alpha DIP13 alpha adapter protein containing PH domain, PTB domain and leucine zipper motif 1 adaptor protein APPL like dip13-alpha
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_145221.2	NP_660256.1	DCC-interacting protein 13-alpha
XM_017316214.2	XP_017171703.1	DCC-interacting protein 13-alpha isoform X2
XM_017316214.1	XP_017171703.1	DCC-interacting protein 13-alpha isoform X2
XM_006519608.1	XP_006519671.1	DCC-interacting protein 13-alpha isoform X1
XM_006519608.2	XP_006519671.1	DCC-interacting protein 13-alpha isoform X1
XM_006519609.3	XP_006519672.1	DCC-interacting protein 13-alpha isoform X3
XM_030248050.1	XP_030103910.1	DCC-interacting protein 13-alpha isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	APPL1	XP_001173707.1
Canis lupus familiaris (dog)	APPL1	XP_849075.1
Gallus gallus (chicken)	APPL1	XP_414344.4
Bos taurus (cattle)	APPL1	NP_001092473.1
Mus musculus (house mouse)	Appl1	NP_660256.1
Homo sapiens (human)	APPL1	NP_036228.1
Rattus norvegicus (Norway rat)	Appl1	XP_006252792.1
Danio rerio (zebrafish)	appl1	NP_001121546.1
Xenopus tropicalis (tropical clawed frog)	appl1	XP_004914231.1

Gene Ontology
Bibliography

Related articles in PubMed

APPL1 prevents pancreatic beta cell death and inflammation by dampening NF?B activation in a mouse model of type 1 diabetes.
Jiang X, Zhou Y, Wu KK, Chen Z, Xu A, Cheng KK
Diabetologia60(3)464-474(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

APPL1 enhances glucose uptake by modulating the activation and localization of PKCzeta, as well as its functional interaction with both PP2A and myosin IIa.
Title: APPL1 promotes glucose uptake in response to mechanical stretch via the PKC?-non-muscle myosin IIa pathway in C2C12 myotubes.

APPL1-SirT1-STAT3 pathway mediated adiponectin signaling in primary hepatocytes.
Title: APPL1-mediated activation of STAT3 contributes to inhibitory effect of adiponectin on hepatic gluconeogenesis.

Here we establish potential roles for APPL1 and 2 signaling adaptors as regulators of LPS/TLR4-induced signaling, transcription, and cytokine secretion.
Title: Distinct Roles for APPL1 and APPL2 in Regulating Toll-like Receptor 4 Signaling in Macrophages.

Appl1/2-null mouse embryonic fibroblasts exhibited defects in HGF-induced Akt activation, migration, and invasion.
Title: Appl1 and Appl2 are Expendable for Mouse Development But Are Essential for HGF-Induced Akt Activation and Migration in Mouse Embryonic Fibroblasts.

In experimental high glucose condition APPL1 acts as a protective factor against podocytes injury through regulating AMPK signaling.
Title: APPL1 acts as a protective factor against podocytes injury in high glucose environment.

The following Appl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Appl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu50393	XM_006519609.3 Latest version!	Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu11409	NM_145221.2 Latest version!	Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu50392	XM_006519608.2 Latest version!	Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu50393	XM_030248050.1 Latest version!	Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu78869	XM_017316214.2 Latest version!	Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu78869	XM_017316214.1	Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OMu50392	XM_006519608.1	Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Appl1 ( XM_006519609.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519672.1 Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu50393
Clone ID Related Accession (Same CDS sequence)	XM_006519609.3 , XM_030248050.1
Accession Version	XM_006519609.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DCC-interacting protein 13-alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519609.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCATT ATTTTTGTGC ACTAAATACT CTTCAGTACA AGAAGAAAAT AGCTTTGTTA 
GAACCTCTAC TGGGATACAT GCAGGCTCAG ATAAGTTTCT TTAAGATGGG TTCTGAAAAT 
CTCAATGGAC AACTGGAAGA ATTCTTAGCT AACATTGGAA CAAGTGTACA GAATGTTCGA 
AGAGAAATGG ATGGTGATGT AGAGACCATG CAGCAGACAA TAGAAGACTT GGAGGTAGCC 
AGTGACCCTT TATATCTGCC TGACCCGGAT CCCACCAAGT TTCCCATCAA TCGAAATTTA 
ACCCGAAAGG CTGGATACCT AAATGCTAGG AATAAAACAG GCTTGGTGTC ATCTACCTGG 
GATAGACAGT TTTACTTCAC GCAGGGTGGA AACTTAATGA GTCAAGCCCG TGGAGATGTG 
GCAGGAGGCC TAGCCATGGA CATAGACAAC TGTTCAGTGA TGGCTGTGGA CTGTGAAGAC 
AGACGATACT GTTTTCAGAT CACTTCTTTT GATGGAAAAA AATCTTCAAT ATTGCAAGCA 
GAGAGTAAAA AAGACCATGA AGAGTGGATC TGTACAATAA ACAACATATC TAAACAAATA 
TATTTGAGTG AAAATCCAGA GGAAACTGCT GCACGAGTGA ATCAGTCTGC TCTAGAAGCT 
GTCACTCCTT CCCCATCTTT CCAGCAGAGA CACGAGAGCC TGCGTCCAGG AGGACAATCT 
CGGCCACCAA CAGCTCGAAC CAGCAGTTCA GGATCCTTGG GATCAGAGTC TACAAATTTG 
GCTGCCCTCT CTCTAGATTC TCTTGTTGCT CCGGATACAC CAATACAGTT TGATATAATT 
TCTCCTGTGT GTGAAGATCA GCCTGGCCAG GCAAAAGCCT TTGGTCAAGG AGGCAGGCGT 
ACAAATCCAT TTGGAGAATC TGGAGGAAGC ACAAAATCAG AAACAGAAGA CTCTATTCTT 
CATCAGTTAT TTATTGTCCG ATTCCTTGGT TCTATGGAGG TAAAGTCAGA TGACCATCCA 
GATGTTGTCT ATGAAACAAT GCGCCAGATC TTAGCTGCTC GGGCCATCCA CAACATCTTT 
CGTATGACAG AATCACATTT ATTAGTCACT TGTGACTGCT TGAAGTTAAT TGATCCGCAG 
ACCCAAGTTA CAAGGCTCAC ATTTCCATTG CCGTGTGTAG TTCTGTATGC TACGCACCAG 
GAAAATAAAC GACTTTTTGG ATTTGTTCTC CGGACATCAG GCGGAAGAAG TGAGAGTAAT 
TTGTCATCAG TCTGCTATAT ATTTGAATCA AACAATGAGG GAGAAAAGAT TTGTGATTCT 
GTTGGACTGG CAAAACAGAT AGCTTTGCAT GCTGAACTGG ATCGTAGGGC ATCAGAAAAA 
CAAAAAGAAA TAGAGAGAGT AAAAGAGAAA CAGCAGAAAG AGCTCAGCAA ACAGAAACAG 
ATTGAGAAGG ACTTGGAAGA ACAGAGTCGG TTGATAGCTG CCTCCAGTAG ACCAAACCAG 
GCTGGTAGTG AGGGCCAGCT TGTTCTTAGC AGTAGCCAAT CAGAGGAGAG TGATTTGGGA 
GAAGAAGGGA AAAAAAGAGA GTCGGAAGCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519672.1
CDS	231..1823
Translation	MMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNGQLEEFLANIGTSVQNVRREMDGDVETMQQTIEDLEVASDPLYLPDPDPTKFPINRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPSFQQRHESLRPGGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQAGSEGQLVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_006519609.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519609.3

ORF Insert Sequence:

ATGATGCATT ATTTTTGTGC ACTAAATACT CTTCAGTACA AGAAGAAAAT AGCTTTGTTA 
GAACCTCTAC TGGGATACAT GCAGGCTCAG ATAAGTTTCT TTAAGATGGG TTCTGAAAAT 
CTCAATGGAC AACTGGAAGA ATTCTTAGCT AACATTGGAA CAAGTGTACA GAATGTTCGA 
AGAGAAATGG ATGGTGATGT AGAGACCATG CAGCAGACAA TAGAAGACTT GGAGGTAGCC 
AGTGACCCTT TATATCTGCC TGACCCGGAT CCCACCAAGT TTCCCATCAA TCGAAATTTA 
ACCCGAAAGG CTGGATACCT AAATGCTAGG AATAAAACAG GCTTGGTGTC ATCTACCTGG 
GATAGACAGT TTTACTTCAC GCAGGGTGGA AACTTAATGA GTCAAGCCCG TGGAGATGTG 
GCAGGAGGCC TAGCCATGGA CATAGACAAC TGTTCAGTGA TGGCTGTGGA CTGTGAAGAC 
AGACGATACT GTTTTCAGAT CACTTCTTTT GATGGAAAAA AATCTTCAAT ATTGCAAGCA 
GAGAGTAAAA AAGACCATGA AGAGTGGATC TGTACAATAA ACAACATATC TAAACAAATA 
TATTTGAGTG AAAATCCAGA GGAAACTGCT GCACGAGTGA ATCAGTCTGC TCTAGAAGCT 
GTCACTCCTT CCCCATCTTT CCAGCAGAGA CACGAGAGCC TGCGTCCAGG AGGACAATCT 
CGGCCACCAA CAGCTCGAAC CAGCAGTTCA GGATCCTTGG GATCAGAGTC TACAAATTTG 
GCTGCCCTCT CTCTAGATTC TCTTGTTGCT CCGGATACAC CAATACAGTT TGATATAATT 
TCTCCTGTGT GTGAAGATCA GCCTGGCCAG GCAAAAGCCT TTGGTCAAGG AGGCAGGCGT 
ACAAATCCAT TTGGAGAATC TGGAGGAAGC ACAAAATCAG AAACAGAAGA CTCTATTCTT 
CATCAGTTAT TTATTGTCCG ATTCCTTGGT TCTATGGAGG TAAAGTCAGA TGACCATCCA 
GATGTTGTCT ATGAAACAAT GCGCCAGATC TTAGCTGCTC GGGCCATCCA CAACATCTTT 
CGTATGACAG AATCACATTT ATTAGTCACT TGTGACTGCT TGAAGTTAAT TGATCCGCAG 
ACCCAAGTTA CAAGGCTCAC ATTTCCATTG CCGTGTGTAG TTCTGTATGC TACGCACCAG 
GAAAATAAAC GACTTTTTGG ATTTGTTCTC CGGACATCAG GCGGAAGAAG TGAGAGTAAT 
TTGTCATCAG TCTGCTATAT ATTTGAATCA AACAATGAGG GAGAAAAGAT TTGTGATTCT 
GTTGGACTGG CAAAACAGAT AGCTTTGCAT GCTGAACTGG ATCGTAGGGC ATCAGAAAAA 
CAAAAAGAAA TAGAGAGAGT AAAAGAGAAA CAGCAGAAAG AGCTCAGCAA ACAGAAACAG 
ATTGAGAAGG ACTTGGAAGA ACAGAGTCGG TTGATAGCTG CCTCCAGTAG ACCAAACCAG 
GCTGGTAGTG AGGGCCAGCT TGTTCTTAGC AGTAGCCAAT CAGAGGAGAG TGATTTGGGA 
GAAGAAGGGA AAAAAAGAGA GTCGGAAGCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Appl1 ( XM_017316214.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171703.1 Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu78869
Clone ID Related Accession (Same CDS sequence)	XM_017316214.2 , XM_017316214.1
Accession Version	XM_017316214.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DCC-interacting protein 13-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTCC CCATTTCCCA GTTTAAAGAA AGAGATCTGA AAGAAATATT GACACTAAAG 
GAGGTATTTC AAATTGCTAG TAATGATCAT GATGCCGCAA TTAATAGGTA CAGTCGGTTG 
TCAAAAAAGA GAGAAAATGA CAAGGTAAAA TATGAAGTTA CAGAGGATGT ATATACTTCC 
AGGAAAAAAC AACACCAGAC CATGATGCAT TATTTTTGTG CACTAAATAC TCTTCAGTAC 
AAGAAGAAAA TAGCTTTGTT AGAACCTCTA CTGGGATACA TGCAGGCTCA GATAAGTTTC 
TTTAAGATGG GTTCTGAAAA TCTCAATGGA CAACTGGAAG AATTCTTAGC TAACATTGGA 
ACAAGTGTAC AGAATGTTCG AAGAGAAATG GATGGTGATG TAGAGACCAT GCAGCAGACA 
ATAGAAGACT TGGAGGTAGC CAGTGACCCT TTATATCTGC CTGACCCGGA TCCCACCAAG 
TTTCCCATCA ATCGAAATTT AACCCGAAAG GCTGGATACC TAAATGCTAG GAATAAAACA 
GGCTTGGTGT CATCTACCTG GGATAGACAG TTTTACTTCA CGCAGGGTGG AAACTTAATG 
AGTCAAGCCC GTGGAGATGT GGCAGGAGGC CTAGCCATGG ACATAGACAA CTGTTCAGTG 
ATGGCTGTGG ACTGTGAAGA CAGACGATAC TGTTTTCAGA TCACTTCTTT TGATGGAAAA 
AAATCTTCAA TATTGCAAGC AGAGAGTAAA AAAGACCATG AAGAGTGGAT CTGTACAATA 
AACAACATAT CTAAACAAAT ATATTTGAGT GAAAATCCAG AGGAAACTGC TGCACGAGTG 
AATCAGTCTG CTCTAGAAGC TGTCACTCCT TCCCCATCTT TCCAGCAGAG ACACGAGAGC 
CTGCGTCCAG GAGGACAATC TCGGCCACCA ACAGCTCGAA CCAGCAGTTC AGGATCCTTG 
GGATCAGAGT CTACAAATTT GGCTGCCCTC TCTCTAGATT CTCTTGTTGC TCCGGATACA 
CCAATACAGT TTGATATAAT TTCTCCTGTG TGTGAAGATC AGCCTGGCCA GGCAAAAGCC 
TTTGGTCAAG GAGGCAGGCG TACAAATCCA TTTGGAGAAT CTGGAGGAAG CACAAAATCA 
GAAACAGAAG ACTCTATTCT TCATCAGTTA TTTATTGTCC GATTCCTTGG TTCTATGGAG 
GTAAAGTCAG ATGACCATCC AGATGTTGTC TATGAAACAA TGCGCCAGAT CTTAGCTGCT 
CGGGCCATCC ACAACATCTT TCGTATGACA GAATCACATT TATTAGTCAC TTGTGACTGC 
TTGAAGTTAA TTGATCCGCA GACCCAAGTT ACAAGGCTCA CATTTCCATT GCCGTGTGTA 
GTTCTGTATG CTACGCACCA GGAAAATAAA CGACTTTTTG GATTTGTTCT CCGGACATCA 
GGCGGAAGAA GTGAGAGTAA TTTGTCATCA GTCTGCTATA TATTTGAATC AAACAATGAG 
GGAGAAAAGA TTTGTGATTC TGTTGGACTG GCAAAACAGA TAGCTTTGCA TGCTGAACTG 
GATCGTAGGG CATCAGAAAA ACAAAAAGAA ATAGAGAGAG TAAAAGAGAA ACAGCAGAAA 
GAGCTCAGCA AACAGAAACA GATTGAGAAG GACTTGGAAG AACAGAGTCG GTTGATAGCT 
GCCTCCAGTA GACCAAACCA GGCTGGTAGT GAGGGCCAGC TTGTTCTTAG CAGTAGCCAA 
TCAGAGGAGA GTGATTTGGG AGAAGAAGGG AAAAAAAGAG AGTCGGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171703.1
CDS	395..2188
Translation	MMFPISQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNGQLEEFLANIGTSVQNVRREMDGDVETMQQTIEDLEVASDPLYLPDPDPTKFPINRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPSFQQRHESLRPGGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQAGSEGQLVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_017316214.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316214.1

ORF Insert Sequence:

ATGATGTTCC CCATTTCCCA GTTTAAAGAA AGAGATCTGA AAGAAATATT GACACTAAAG 
GAGGTATTTC AAATTGCTAG TAATGATCAT GATGCCGCAA TTAATAGGTA CAGTCGGTTG 
TCAAAAAAGA GAGAAAATGA CAAGGTAAAA TATGAAGTTA CAGAGGATGT ATATACTTCC 
AGGAAAAAAC AACACCAGAC CATGATGCAT TATTTTTGTG CACTAAATAC TCTTCAGTAC 
AAGAAGAAAA TAGCTTTGTT AGAACCTCTA CTGGGATACA TGCAGGCTCA GATAAGTTTC 
TTTAAGATGG GTTCTGAAAA TCTCAATGGA CAACTGGAAG AATTCTTAGC TAACATTGGA 
ACAAGTGTAC AGAATGTTCG AAGAGAAATG GATGGTGATG TAGAGACCAT GCAGCAGACA 
ATAGAAGACT TGGAGGTAGC CAGTGACCCT TTATATCTGC CTGACCCGGA TCCCACCAAG 
TTTCCCATCA ATCGAAATTT AACCCGAAAG GCTGGATACC TAAATGCTAG GAATAAAACA 
GGCTTGGTGT CATCTACCTG GGATAGACAG TTTTACTTCA CGCAGGGTGG AAACTTAATG 
AGTCAAGCCC GTGGAGATGT GGCAGGAGGC CTAGCCATGG ACATAGACAA CTGTTCAGTG 
ATGGCTGTGG ACTGTGAAGA CAGACGATAC TGTTTTCAGA TCACTTCTTT TGATGGAAAA 
AAATCTTCAA TATTGCAAGC AGAGAGTAAA AAAGACCATG AAGAGTGGAT CTGTACAATA 
AACAACATAT CTAAACAAAT ATATTTGAGT GAAAATCCAG AGGAAACTGC TGCACGAGTG 
AATCAGTCTG CTCTAGAAGC TGTCACTCCT TCCCCATCTT TCCAGCAGAG ACACGAGAGC 
CTGCGTCCAG GAGGACAATC TCGGCCACCA ACAGCTCGAA CCAGCAGTTC AGGATCCTTG 
GGATCAGAGT CTACAAATTT GGCTGCCCTC TCTCTAGATT CTCTTGTTGC TCCGGATACA 
CCAATACAGT TTGATATAAT TTCTCCTGTG TGTGAAGATC AGCCTGGCCA GGCAAAAGCC 
TTTGGTCAAG GAGGCAGGCG TACAAATCCA TTTGGAGAAT CTGGAGGAAG CACAAAATCA 
GAAACAGAAG ACTCTATTCT TCATCAGTTA TTTATTGTCC GATTCCTTGG TTCTATGGAG 
GTAAAGTCAG ATGACCATCC AGATGTTGTC TATGAAACAA TGCGCCAGAT CTTAGCTGCT 
CGGGCCATCC ACAACATCTT TCGTATGACA GAATCACATT TATTAGTCAC TTGTGACTGC 
TTGAAGTTAA TTGATCCGCA GACCCAAGTT ACAAGGCTCA CATTTCCATT GCCGTGTGTA 
GTTCTGTATG CTACGCACCA GGAAAATAAA CGACTTTTTG GATTTGTTCT CCGGACATCA 
GGCGGAAGAA GTGAGAGTAA TTTGTCATCA GTCTGCTATA TATTTGAATC AAACAATGAG 
GGAGAAAAGA TTTGTGATTC TGTTGGACTG GCAAAACAGA TAGCTTTGCA TGCTGAACTG 
GATCGTAGGG CATCAGAAAA ACAAAAAGAA ATAGAGAGAG TAAAAGAGAA ACAGCAGAAA 
GAGCTCAGCA AACAGAAACA GATTGAGAAG GACTTGGAAG AACAGAGTCG GTTGATAGCT 
GCCTCCAGTA GACCAAACCA GGCTGGTAGT GAGGGCCAGC TTGTTCTTAG CAGTAGCCAA 
TCAGAGGAGA GTGATTTGGG AGAAGAAGGG AAAAAAAGAG AGTCGGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Appl1 ( XM_006519608.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519671.1 Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu50392
Clone ID Related Accession (Same CDS sequence)	XM_006519608.1 , XM_006519608.2
Accession Version	XM_006519608.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DCC-interacting protein 13-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGA TCGACAAGCT GCCCATTGAA GAGACCCTGG AGGACAGTCC GCAGACAAGG 
TCTTTACTTG GTGTATTTGA AGAAGATGCT ACTGCTATTT CAAATTATAT GAACCAATTG 
TATCAAGCTA TGCATCGGAT TTATGATGCA CAGAATGAAT TGAGTGCAGC AACACACCTG 
ACTTCGAAAC TTTTGAAAGA ATATGAAAAA CAGCTTAGTT CTTGTCATGC AGTACTTTCA 
ACTCAACTTG CTGATGCCAT GATGTTCCCC ATTTCCCAGT TTAAAGAAAG AGATCTGAAA 
GAAATATTGA CACTAAAGGA GGTATTTCAA ATTGCTAGTA ATGATCATGA TGCCGCAATT 
AATAGGTACA GTCGGTTGTC AAAAAAGAGA GAAAATGACA AGGTAAAATA TGAAGTTACA 
GAGGATGTAT ATACTTCCAG GAAAAAACAA CACCAGACCA TGATGCATTA TTTTTGTGCA 
CTAAATACTC TTCAGTACAA GAAGAAAATA GCTTTGTTAG AACCTCTACT GGGATACATG 
CAGGCTCAGA TAAGTTTCTT TAAGATGGGT TCTGAAAATC TCAATGGACA ACTGGAAGAA 
TTCTTAGCTA ACATTGGAAC AAGTGTACAG AATGTTCGAA GAGAAATGGA TGGTGATGTA 
GAGACCATGC AGCAGACAAT AGAAGACTTG GAGGTAGCCA GTGACCCTTT ATATCTGCCT 
GACCCGGATC CCACCAAGTT TCCCATCAAT CGAAATTTAA CCCGAAAGGC TGGATACCTA 
AATGCTAGGA ATAAAACAGG CTTGGTGTCA TCTACCTGGG ATAGACAGTT TTACTTCACG 
CAGGGTGGAA ACTTAATGAG TCAAGCCCGT GGAGATGTGG CAGGAGGCCT AGCCATGGAC 
ATAGACAACT GTTCAGTGAT GGCTGTGGAC TGTGAAGACA GACGATACTG TTTTCAGATC 
ACTTCTTTTG ATGGAAAAAA ATCTTCAATA TTGCAAGCAG AGAGTAAAAA AGACCATGAA 
GAGTGGATCT GTACAATAAA CAACATATCT AAACAAATAT ATTTGAGTGA AAATCCAGAG 
GAAACTGCTG CACGAGTGAA TCAGTCTGCT CTAGAAGCTG TCACTCCTTC CCCATCTTTC 
CAGCAGAGAC ACGAGAGCCT GCGTCCAGGA GGACAATCTC GGCCACCAAC AGCTCGAACC 
AGCAGTTCAG GATCCTTGGG ATCAGAGTCT ACAAATTTGG CTGCCCTCTC TCTAGATTCT 
CTTGTTGCTC CGGATACACC AATACAGTTT GATATAATTT CTCCTGTGTG TGAAGATCAG 
CCTGGCCAGG CAAAAGCCTT TGGTCAAGGA GGCAGGCGTA CAAATCCATT TGGAGAATCT 
GGAGGAAGCA CAAAATCAGA AACAGAAGAC TCTATTCTTC ATCAGTTATT TATTGTCCGA 
TTCCTTGGTT CTATGGAGGT AAAGTCAGAT GACCATCCAG ATGTTGTCTA TGAAACAATG 
CGCCAGATCT TAGCTGCTCG GGCCATCCAC AACATCTTTC GTATGACAGA ATCACATTTA 
TTAGTCACTT GTGACTGCTT GAAGTTAATT GATCCGCAGA CCCAAGTTAC AAGGCTCACA 
TTTCCATTGC CGTGTGTAGT TCTGTATGCT ACGCACCAGG AAAATAAACG ACTTTTTGGA 
TTTGTTCTCC GGACATCAGG CGGAAGAAGT GAGAGTAATT TGTCATCAGT CTGCTATATA 
TTTGAATCAA ACAATGAGGG AGAAAAGATT TGTGATTCTG TTGGACTGGC AAAACAGATA 
GCTTTGCATG CTGAACTGGA TCGTAGGGCA TCAGAAAAAC AAAAAGAAAT AGAGAGAGTA 
AAAGAGAAAC AGCAGAAAGA GCTCAGCAAA CAGAAACAGA TTGAGAAGGA CTTGGAAGAA 
CAGAGTCGGT TGATAGCTGC CTCCAGTAGA CCAAACCAGG CTGGTAGTGA GGGCCAGCTT 
GTTCTTAGCA GTAGCCAATC AGAGGAGAGT GATTTGGGAG AAGAAGGGAA AAAAAGAGAG 
TCGGAAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519671.1
CDS	752..2803
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMHRIYDAQNELSAATHLTSKLLKEYEKQLSSCHAVLSTQLADAMMFPISQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNGQLEEFLANIGTSVQNVRREMDGDVETMQQTIEDLEVASDPLYLPDPDPTKFPINRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPSFQQRHESLRPGGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQAGSEGQLVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_006519608.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519608.1

ORF Insert Sequence:

ATGCCGGGGA TCGACAAGCT GCCCATTGAA GAGACCCTGG AGGACAGTCC GCAGACAAGG 
TCTTTACTTG GTGTATTTGA AGAAGATGCT ACTGCTATTT CAAATTATAT GAACCAATTG 
TATCAAGCTA TGCATCGGAT TTATGATGCA CAGAATGAAT TGAGTGCAGC AACACACCTG 
ACTTCGAAAC TTTTGAAAGA ATATGAAAAA CAGCTTAGTT CTTGTCATGC AGTACTTTCA 
ACTCAACTTG CTGATGCCAT GATGTTCCCC ATTTCCCAGT TTAAAGAAAG AGATCTGAAA 
GAAATATTGA CACTAAAGGA GGTATTTCAA ATTGCTAGTA ATGATCATGA TGCCGCAATT 
AATAGGTACA GTCGGTTGTC AAAAAAGAGA GAAAATGACA AGGTAAAATA TGAAGTTACA 
GAGGATGTAT ATACTTCCAG GAAAAAACAA CACCAGACCA TGATGCATTA TTTTTGTGCA 
CTAAATACTC TTCAGTACAA GAAGAAAATA GCTTTGTTAG AACCTCTACT GGGATACATG 
CAGGCTCAGA TAAGTTTCTT TAAGATGGGT TCTGAAAATC TCAATGGACA ACTGGAAGAA 
TTCTTAGCTA ACATTGGAAC AAGTGTACAG AATGTTCGAA GAGAAATGGA TGGTGATGTA 
GAGACCATGC AGCAGACAAT AGAAGACTTG GAGGTAGCCA GTGACCCTTT ATATCTGCCT 
GACCCGGATC CCACCAAGTT TCCCATCAAT CGAAATTTAA CCCGAAAGGC TGGATACCTA 
AATGCTAGGA ATAAAACAGG CTTGGTGTCA TCTACCTGGG ATAGACAGTT TTACTTCACG 
CAGGGTGGAA ACTTAATGAG TCAAGCCCGT GGAGATGTGG CAGGAGGCCT AGCCATGGAC 
ATAGACAACT GTTCAGTGAT GGCTGTGGAC TGTGAAGACA GACGATACTG TTTTCAGATC 
ACTTCTTTTG ATGGAAAAAA ATCTTCAATA TTGCAAGCAG AGAGTAAAAA AGACCATGAA 
GAGTGGATCT GTACAATAAA CAACATATCT AAACAAATAT ATTTGAGTGA AAATCCAGAG 
GAAACTGCTG CACGAGTGAA TCAGTCTGCT CTAGAAGCTG TCACTCCTTC CCCATCTTTC 
CAGCAGAGAC ACGAGAGCCT GCGTCCAGGA GGACAATCTC GGCCACCAAC AGCTCGAACC 
AGCAGTTCAG GATCCTTGGG ATCAGAGTCT ACAAATTTGG CTGCCCTCTC TCTAGATTCT 
CTTGTTGCTC CGGATACACC AATACAGTTT GATATAATTT CTCCTGTGTG TGAAGATCAG 
CCTGGCCAGG CAAAAGCCTT TGGTCAAGGA GGCAGGCGTA CAAATCCATT TGGAGAATCT 
GGAGGAAGCA CAAAATCAGA AACAGAAGAC TCTATTCTTC ATCAGTTATT TATTGTCCGA 
TTCCTTGGTT CTATGGAGGT AAAGTCAGAT GACCATCCAG ATGTTGTCTA TGAAACAATG 
CGCCAGATCT TAGCTGCTCG GGCCATCCAC AACATCTTTC GTATGACAGA ATCACATTTA 
TTAGTCACTT GTGACTGCTT GAAGTTAATT GATCCGCAGA CCCAAGTTAC AAGGCTCACA 
TTTCCATTGC CGTGTGTAGT TCTGTATGCT ACGCACCAGG AAAATAAACG ACTTTTTGGA 
TTTGTTCTCC GGACATCAGG CGGAAGAAGT GAGAGTAATT TGTCATCAGT CTGCTATATA 
TTTGAATCAA ACAATGAGGG AGAAAAGATT TGTGATTCTG TTGGACTGGC AAAACAGATA 
GCTTTGCATG CTGAACTGGA TCGTAGGGCA TCAGAAAAAC AAAAAGAAAT AGAGAGAGTA 
AAAGAGAAAC AGCAGAAAGA GCTCAGCAAA CAGAAACAGA TTGAGAAGGA CTTGGAAGAA 
CAGAGTCGGT TGATAGCTGC CTCCAGTAGA CCAAACCAGG CTGGTAGTGA GGGCCAGCTT 
GTTCTTAGCA GTAGCCAATC AGAGGAGAGT GATTTGGGAG AAGAAGGGAA AAAAAGAGAG 
TCGGAAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Appl1 ( NM_145221.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_660256.1 Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), mRNA.

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CloneID	OMu11409
Clone ID Related Accession (Same CDS sequence)	NM_145221.2
Accession Version	NM_145221.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Mus musculus(house mouse)
Product	DCC-interacting protein 13-alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU514763.1, AK033566.1, AK173169.1 and AK037695.1. On or before May 4, 2006 this sequence version replaced XM_975639.1, NM_145221.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.62488.1, AK030445.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGGGA TCGACAAGCT GCCCATTGAA GAGACCCTGG AGGACAGTCC GCAGACAAGG 
TCTTTACTTG GTGTATTTGA AGAAGATGCT ACTGCTATTT CAAATTATAT GAACCAATTG 
TATCAAGCTA TGCATCGGAT TTATGATGCA CAGAATGAAT TGAGTGCAGC AACACACCTG 
ACTTCGAAAC TTTTGAAAGA ATATGAAAAA CAGCGTTTTC CTTTGGGGGG TGATGATGAA 
GTTATGAGCT CTACTTTGCA GCAGTTTTCC AAGGTCATAG ATGAGCTTAG TTCTTGTCAT 
GCAGTACTTT CAACTCAACT TGCTGATGCC ATGATGTTCC CCATTTCCCA GTTTAAAGAA 
AGAGATCTGA AAGAAATATT GACACTAAAG GAGGTATTTC AAATTGCTAG TAATGATCAT 
GATGCCGCAA TTAATAGGTA CAGTCGGTTG TCAAAAAAGA GAGAAAATGA CAAGGTAAAA 
TATGAAGTTA CAGAGGATGT ATATACTTCC AGGAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CACTAAATAC TCTTCAGTAC AAGAAGAAAA TAGCTTTGTT AGAACCTCTA 
CTGGGATACA TGCAGGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTCAATGGA 
CAACTGGAAG AATTCTTAGC TAACATTGGA ACAAGTGTAC AGAATGTTCG AAGAGAAATG 
GATGGTGATG TAGAGACCAT GCAGCAGACA ATAGAAGACT TGGAGGTAGC CAGTGACCCT 
TTATATCTGC CTGACCCGGA TCCCACCAAG TTTCCCATCA ATCGAAATTT AACCCGAAAG 
GCTGGATACC TAAATGCTAG GAATAAAACA GGCTTGGTGT CATCTACCTG GGATAGACAG 
TTTTACTTCA CGCAGGGTGG AAACTTAATG AGTCAAGCCC GTGGAGATGT GGCAGGAGGC 
CTAGCCATGG ACATAGACAA CTGTTCAGTG ATGGCTGTGG ACTGTGAAGA CAGACGATAC 
TGTTTTCAGA TCACTTCTTT TGATGGAAAA AAATCTTCAA TATTGCAAGC AGAGAGTAAA 
AAAGACCATG AAGAGTGGAT CTGTACAATA AACAACATAT CTAAACAAAT ATATTTGAGT 
GAAAATCCAG AGGAAACTGC TGCACGAGTG AATCAGTCTG CTCTAGAAGC TGTCACTCCT 
TCCCCATCTT TCCAGCAGAG ACACGAGAGC CTGCGTCCAG GAGGACAATC TCGGCCACCA 
ACAGCTCGAA CCAGCAGTTC AGGATCCTTG GGATCAGAGT CTACAAATTT GGCTGCCCTC 
TCTCTAGATT CTCTTGTTGC TCCGGATACA CCAATACAGT TTGATATAAT TTCTCCTGTG 
TGTGAAGATC AGCCTGGCCA GGCAAAAGCC TTTGGTCAAG GAGGCAGGCG TACAAATCCA 
TTTGGAGAAT CTGGAGGAAG CACAAAATCA GAAACAGAAG ACTCTATTCT TCATCAGTTA 
TTTATTGTCC GATTCCTTGG TTCTATGGAG GTAAAGTCAG ATGACCATCC AGATGTTGTC 
TATGAAACAA TGCGCCAGAT CTTAGCTGCT CGGGCCATCC ACAACATCTT TCGTATGACA 
GAATCACATT TATTAGTCAC TTGTGACTGC TTGAAGTTAA TTGATCCGCA GACCCAAGTT 
ACAAGGCTCA CATTTCCATT GCCGTGTGTA GTTCTGTATG CTACGCACCA GGAAAATAAA 
CGACTTTTTG GATTTGTTCT CCGGACATCA GGCGGAAGAA GTGAGAGTAA TTTGTCATCA 
GTCTGCTATA TATTTGAATC AAACAATGAG GGAGAAAAGA TTTGTGATTC TGTTGGACTG 
GCAAAACAGA TAGCTTTGCA TGCTGAACTG GATCGTAGGG CATCAGAAAA ACAAAAAGAA 
ATAGAGAGAG TAAAAGAGAA ACAGCAGAAA GAGCTCAGCA AACAGAAACA GATTGAGAAG 
GACTTGGAAG AACAGAGTCG GTTGATAGCT GCCTCCAGTA GACCAAACCA GGCTGGTAGT 
GAGGGCCAGC TTGTTCTTAG CAGTAGCCAA TCAGAGGAGA GTGATTTGGG AGAAGAAGGG 
AAAAAAAGAG AGTCGGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660256.1
CDS	98..2221
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMHRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPISQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNGQLEEFLANIGTSVQNVRREMDGDVETMQQTIEDLEVASDPLYLPDPDPTKFPINRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPSFQQRHESLRPGGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQAGSEGQLVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	NM_145221.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145221.2

ORF Insert Sequence:

ATGCCGGGGA TCGACAAGCT GCCCATTGAA GAGACCCTGG AGGACAGTCC GCAGACAAGG 
TCTTTACTTG GTGTATTTGA AGAAGATGCT ACTGCTATTT CAAATTATAT GAACCAATTG 
TATCAAGCTA TGCATCGGAT TTATGATGCA CAGAATGAAT TGAGTGCAGC AACACACCTG 
ACTTCGAAAC TTTTGAAAGA ATATGAAAAA CAGCGTTTTC CTTTGGGGGG TGATGATGAA 
GTTATGAGCT CTACTTTGCA GCAGTTTTCC AAGGTCATAG ATGAGCTTAG TTCTTGTCAT 
GCAGTACTTT CAACTCAACT TGCTGATGCC ATGATGTTCC CCATTTCCCA GTTTAAAGAA 
AGAGATCTGA AAGAAATATT GACACTAAAG GAGGTATTTC AAATTGCTAG TAATGATCAT 
GATGCCGCAA TTAATAGGTA CAGTCGGTTG TCAAAAAAGA GAGAAAATGA CAAGGTAAAA 
TATGAAGTTA CAGAGGATGT ATATACTTCC AGGAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CACTAAATAC TCTTCAGTAC AAGAAGAAAA TAGCTTTGTT AGAACCTCTA 
CTGGGATACA TGCAGGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTCAATGGA 
CAACTGGAAG AATTCTTAGC TAACATTGGA ACAAGTGTAC AGAATGTTCG AAGAGAAATG 
GATGGTGATG TAGAGACCAT GCAGCAGACA ATAGAAGACT TGGAGGTAGC CAGTGACCCT 
TTATATCTGC CTGACCCGGA TCCCACCAAG TTTCCCATCA ATCGAAATTT AACCCGAAAG 
GCTGGATACC TAAATGCTAG GAATAAAACA GGCTTGGTGT CATCTACCTG GGATAGACAG 
TTTTACTTCA CGCAGGGTGG AAACTTAATG AGTCAAGCCC GTGGAGATGT GGCAGGAGGC 
CTAGCCATGG ACATAGACAA CTGTTCAGTG ATGGCTGTGG ACTGTGAAGA CAGACGATAC 
TGTTTTCAGA TCACTTCTTT TGATGGAAAA AAATCTTCAA TATTGCAAGC AGAGAGTAAA 
AAAGACCATG AAGAGTGGAT CTGTACAATA AACAACATAT CTAAACAAAT ATATTTGAGT 
GAAAATCCAG AGGAAACTGC TGCACGAGTG AATCAGTCTG CTCTAGAAGC TGTCACTCCT 
TCCCCATCTT TCCAGCAGAG ACACGAGAGC CTGCGTCCAG GAGGACAATC TCGGCCACCA 
ACAGCTCGAA CCAGCAGTTC AGGATCCTTG GGATCAGAGT CTACAAATTT GGCTGCCCTC 
TCTCTAGATT CTCTTGTTGC TCCGGATACA CCAATACAGT TTGATATAAT TTCTCCTGTG 
TGTGAAGATC AGCCTGGCCA GGCAAAAGCC TTTGGTCAAG GAGGCAGGCG TACAAATCCA 
TTTGGAGAAT CTGGAGGAAG CACAAAATCA GAAACAGAAG ACTCTATTCT TCATCAGTTA 
TTTATTGTCC GATTCCTTGG TTCTATGGAG GTAAAGTCAG ATGACCATCC AGATGTTGTC 
TATGAAACAA TGCGCCAGAT CTTAGCTGCT CGGGCCATCC ACAACATCTT TCGTATGACA 
GAATCACATT TATTAGTCAC TTGTGACTGC TTGAAGTTAA TTGATCCGCA GACCCAAGTT 
ACAAGGCTCA CATTTCCATT GCCGTGTGTA GTTCTGTATG CTACGCACCA GGAAAATAAA 
CGACTTTTTG GATTTGTTCT CCGGACATCA GGCGGAAGAA GTGAGAGTAA TTTGTCATCA 
GTCTGCTATA TATTTGAATC AAACAATGAG GGAGAAAAGA TTTGTGATTC TGTTGGACTG 
GCAAAACAGA TAGCTTTGCA TGCTGAACTG GATCGTAGGG CATCAGAAAA ACAAAAAGAA 
ATAGAGAGAG TAAAAGAGAA ACAGCAGAAA GAGCTCAGCA AACAGAAACA GATTGAGAAG 
GACTTGGAAG AACAGAGTCG GTTGATAGCT GCCTCCAGTA GACCAAACCA GGCTGGTAGT 
GAGGGCCAGC TTGTTCTTAG CAGTAGCCAA TCAGAGGAGA GTGATTTGGG AGAAGAAGGG 
AAAAAAAGAG AGTCGGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Appl1 ( XM_006519608.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519671.1 Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu50392
Clone ID Related Accession (Same CDS sequence)	XM_006519608.1 , XM_006519608.2
Accession Version	XM_006519608.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DCC-interacting protein 13-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006519608.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGA TCGACAAGCT GCCCATTGAA GAGACCCTGG AGGACAGTCC GCAGACAAGG 
TCTTTACTTG GTGTATTTGA AGAAGATGCT ACTGCTATTT CAAATTATAT GAACCAATTG 
TATCAAGCTA TGCATCGGAT TTATGATGCA CAGAATGAAT TGAGTGCAGC AACACACCTG 
ACTTCGAAAC TTTTGAAAGA ATATGAAAAA CAGCTTAGTT CTTGTCATGC AGTACTTTCA 
ACTCAACTTG CTGATGCCAT GATGTTCCCC ATTTCCCAGT TTAAAGAAAG AGATCTGAAA 
GAAATATTGA CACTAAAGGA GGTATTTCAA ATTGCTAGTA ATGATCATGA TGCCGCAATT 
AATAGGTACA GTCGGTTGTC AAAAAAGAGA GAAAATGACA AGGTAAAATA TGAAGTTACA 
GAGGATGTAT ATACTTCCAG GAAAAAACAA CACCAGACCA TGATGCATTA TTTTTGTGCA 
CTAAATACTC TTCAGTACAA GAAGAAAATA GCTTTGTTAG AACCTCTACT GGGATACATG 
CAGGCTCAGA TAAGTTTCTT TAAGATGGGT TCTGAAAATC TCAATGGACA ACTGGAAGAA 
TTCTTAGCTA ACATTGGAAC AAGTGTACAG AATGTTCGAA GAGAAATGGA TGGTGATGTA 
GAGACCATGC AGCAGACAAT AGAAGACTTG GAGGTAGCCA GTGACCCTTT ATATCTGCCT 
GACCCGGATC CCACCAAGTT TCCCATCAAT CGAAATTTAA CCCGAAAGGC TGGATACCTA 
AATGCTAGGA ATAAAACAGG CTTGGTGTCA TCTACCTGGG ATAGACAGTT TTACTTCACG 
CAGGGTGGAA ACTTAATGAG TCAAGCCCGT GGAGATGTGG CAGGAGGCCT AGCCATGGAC 
ATAGACAACT GTTCAGTGAT GGCTGTGGAC TGTGAAGACA GACGATACTG TTTTCAGATC 
ACTTCTTTTG ATGGAAAAAA ATCTTCAATA TTGCAAGCAG AGAGTAAAAA AGACCATGAA 
GAGTGGATCT GTACAATAAA CAACATATCT AAACAAATAT ATTTGAGTGA AAATCCAGAG 
GAAACTGCTG CACGAGTGAA TCAGTCTGCT CTAGAAGCTG TCACTCCTTC CCCATCTTTC 
CAGCAGAGAC ACGAGAGCCT GCGTCCAGGA GGACAATCTC GGCCACCAAC AGCTCGAACC 
AGCAGTTCAG GATCCTTGGG ATCAGAGTCT ACAAATTTGG CTGCCCTCTC TCTAGATTCT 
CTTGTTGCTC CGGATACACC AATACAGTTT GATATAATTT CTCCTGTGTG TGAAGATCAG 
CCTGGCCAGG CAAAAGCCTT TGGTCAAGGA GGCAGGCGTA CAAATCCATT TGGAGAATCT 
GGAGGAAGCA CAAAATCAGA AACAGAAGAC TCTATTCTTC ATCAGTTATT TATTGTCCGA 
TTCCTTGGTT CTATGGAGGT AAAGTCAGAT GACCATCCAG ATGTTGTCTA TGAAACAATG 
CGCCAGATCT TAGCTGCTCG GGCCATCCAC AACATCTTTC GTATGACAGA ATCACATTTA 
TTAGTCACTT GTGACTGCTT GAAGTTAATT GATCCGCAGA CCCAAGTTAC AAGGCTCACA 
TTTCCATTGC CGTGTGTAGT TCTGTATGCT ACGCACCAGG AAAATAAACG ACTTTTTGGA 
TTTGTTCTCC GGACATCAGG CGGAAGAAGT GAGAGTAATT TGTCATCAGT CTGCTATATA 
TTTGAATCAA ACAATGAGGG AGAAAAGATT TGTGATTCTG TTGGACTGGC AAAACAGATA 
GCTTTGCATG CTGAACTGGA TCGTAGGGCA TCAGAAAAAC AAAAAGAAAT AGAGAGAGTA 
AAAGAGAAAC AGCAGAAAGA GCTCAGCAAA CAGAAACAGA TTGAGAAGGA CTTGGAAGAA 
CAGAGTCGGT TGATAGCTGC CTCCAGTAGA CCAAACCAGG CTGGTAGTGA GGGCCAGCTT 
GTTCTTAGCA GTAGCCAATC AGAGGAGAGT GATTTGGGAG AAGAAGGGAA AAAAAGAGAG 
TCGGAAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519671.1
CDS	134..2185
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMHRIYDAQNELSAATHLTSKLLKEYEKQLSSCHAVLSTQLADAMMFPISQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNGQLEEFLANIGTSVQNVRREMDGDVETMQQTIEDLEVASDPLYLPDPDPTKFPINRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPSFQQRHESLRPGGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQAGSEGQLVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_006519608.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519608.2

ORF Insert Sequence:

ATGCCGGGGA TCGACAAGCT GCCCATTGAA GAGACCCTGG AGGACAGTCC GCAGACAAGG 
TCTTTACTTG GTGTATTTGA AGAAGATGCT ACTGCTATTT CAAATTATAT GAACCAATTG 
TATCAAGCTA TGCATCGGAT TTATGATGCA CAGAATGAAT TGAGTGCAGC AACACACCTG 
ACTTCGAAAC TTTTGAAAGA ATATGAAAAA CAGCTTAGTT CTTGTCATGC AGTACTTTCA 
ACTCAACTTG CTGATGCCAT GATGTTCCCC ATTTCCCAGT TTAAAGAAAG AGATCTGAAA 
GAAATATTGA CACTAAAGGA GGTATTTCAA ATTGCTAGTA ATGATCATGA TGCCGCAATT 
AATAGGTACA GTCGGTTGTC AAAAAAGAGA GAAAATGACA AGGTAAAATA TGAAGTTACA 
GAGGATGTAT ATACTTCCAG GAAAAAACAA CACCAGACCA TGATGCATTA TTTTTGTGCA 
CTAAATACTC TTCAGTACAA GAAGAAAATA GCTTTGTTAG AACCTCTACT GGGATACATG 
CAGGCTCAGA TAAGTTTCTT TAAGATGGGT TCTGAAAATC TCAATGGACA ACTGGAAGAA 
TTCTTAGCTA ACATTGGAAC AAGTGTACAG AATGTTCGAA GAGAAATGGA TGGTGATGTA 
GAGACCATGC AGCAGACAAT AGAAGACTTG GAGGTAGCCA GTGACCCTTT ATATCTGCCT 
GACCCGGATC CCACCAAGTT TCCCATCAAT CGAAATTTAA CCCGAAAGGC TGGATACCTA 
AATGCTAGGA ATAAAACAGG CTTGGTGTCA TCTACCTGGG ATAGACAGTT TTACTTCACG 
CAGGGTGGAA ACTTAATGAG TCAAGCCCGT GGAGATGTGG CAGGAGGCCT AGCCATGGAC 
ATAGACAACT GTTCAGTGAT GGCTGTGGAC TGTGAAGACA GACGATACTG TTTTCAGATC 
ACTTCTTTTG ATGGAAAAAA ATCTTCAATA TTGCAAGCAG AGAGTAAAAA AGACCATGAA 
GAGTGGATCT GTACAATAAA CAACATATCT AAACAAATAT ATTTGAGTGA AAATCCAGAG 
GAAACTGCTG CACGAGTGAA TCAGTCTGCT CTAGAAGCTG TCACTCCTTC CCCATCTTTC 
CAGCAGAGAC ACGAGAGCCT GCGTCCAGGA GGACAATCTC GGCCACCAAC AGCTCGAACC 
AGCAGTTCAG GATCCTTGGG ATCAGAGTCT ACAAATTTGG CTGCCCTCTC TCTAGATTCT 
CTTGTTGCTC CGGATACACC AATACAGTTT GATATAATTT CTCCTGTGTG TGAAGATCAG 
CCTGGCCAGG CAAAAGCCTT TGGTCAAGGA GGCAGGCGTA CAAATCCATT TGGAGAATCT 
GGAGGAAGCA CAAAATCAGA AACAGAAGAC TCTATTCTTC ATCAGTTATT TATTGTCCGA 
TTCCTTGGTT CTATGGAGGT AAAGTCAGAT GACCATCCAG ATGTTGTCTA TGAAACAATG 
CGCCAGATCT TAGCTGCTCG GGCCATCCAC AACATCTTTC GTATGACAGA ATCACATTTA 
TTAGTCACTT GTGACTGCTT GAAGTTAATT GATCCGCAGA CCCAAGTTAC AAGGCTCACA 
TTTCCATTGC CGTGTGTAGT TCTGTATGCT ACGCACCAGG AAAATAAACG ACTTTTTGGA 
TTTGTTCTCC GGACATCAGG CGGAAGAAGT GAGAGTAATT TGTCATCAGT CTGCTATATA 
TTTGAATCAA ACAATGAGGG AGAAAAGATT TGTGATTCTG TTGGACTGGC AAAACAGATA 
GCTTTGCATG CTGAACTGGA TCGTAGGGCA TCAGAAAAAC AAAAAGAAAT AGAGAGAGTA 
AAAGAGAAAC AGCAGAAAGA GCTCAGCAAA CAGAAACAGA TTGAGAAGGA CTTGGAAGAA 
CAGAGTCGGT TGATAGCTGC CTCCAGTAGA CCAAACCAGG CTGGTAGTGA GGGCCAGCTT 
GTTCTTAGCA GTAGCCAATC AGAGGAGAGT GATTTGGGAG AAGAAGGGAA AAAAAGAGAG 
TCGGAAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Appl1 ( XM_030248050.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103910.1 Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X3, mRNA.

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CloneID	OMu50393
Clone ID Related Accession (Same CDS sequence)	XM_006519609.3 , XM_030248050.1
Accession Version	XM_030248050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DCC-interacting protein 13-alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCATT ATTTTTGTGC ACTAAATACT CTTCAGTACA AGAAGAAAAT AGCTTTGTTA 
GAACCTCTAC TGGGATACAT GCAGGCTCAG ATAAGTTTCT TTAAGATGGG TTCTGAAAAT 
CTCAATGGAC AACTGGAAGA ATTCTTAGCT AACATTGGAA CAAGTGTACA GAATGTTCGA 
AGAGAAATGG ATGGTGATGT AGAGACCATG CAGCAGACAA TAGAAGACTT GGAGGTAGCC 
AGTGACCCTT TATATCTGCC TGACCCGGAT CCCACCAAGT TTCCCATCAA TCGAAATTTA 
ACCCGAAAGG CTGGATACCT AAATGCTAGG AATAAAACAG GCTTGGTGTC ATCTACCTGG 
GATAGACAGT TTTACTTCAC GCAGGGTGGA AACTTAATGA GTCAAGCCCG TGGAGATGTG 
GCAGGAGGCC TAGCCATGGA CATAGACAAC TGTTCAGTGA TGGCTGTGGA CTGTGAAGAC 
AGACGATACT GTTTTCAGAT CACTTCTTTT GATGGAAAAA AATCTTCAAT ATTGCAAGCA 
GAGAGTAAAA AAGACCATGA AGAGTGGATC TGTACAATAA ACAACATATC TAAACAAATA 
TATTTGAGTG AAAATCCAGA GGAAACTGCT GCACGAGTGA ATCAGTCTGC TCTAGAAGCT 
GTCACTCCTT CCCCATCTTT CCAGCAGAGA CACGAGAGCC TGCGTCCAGG AGGACAATCT 
CGGCCACCAA CAGCTCGAAC CAGCAGTTCA GGATCCTTGG GATCAGAGTC TACAAATTTG 
GCTGCCCTCT CTCTAGATTC TCTTGTTGCT CCGGATACAC CAATACAGTT TGATATAATT 
TCTCCTGTGT GTGAAGATCA GCCTGGCCAG GCAAAAGCCT TTGGTCAAGG AGGCAGGCGT 
ACAAATCCAT TTGGAGAATC TGGAGGAAGC ACAAAATCAG AAACAGAAGA CTCTATTCTT 
CATCAGTTAT TTATTGTCCG ATTCCTTGGT TCTATGGAGG TAAAGTCAGA TGACCATCCA 
GATGTTGTCT ATGAAACAAT GCGCCAGATC TTAGCTGCTC GGGCCATCCA CAACATCTTT 
CGTATGACAG AATCACATTT ATTAGTCACT TGTGACTGCT TGAAGTTAAT TGATCCGCAG 
ACCCAAGTTA CAAGGCTCAC ATTTCCATTG CCGTGTGTAG TTCTGTATGC TACGCACCAG 
GAAAATAAAC GACTTTTTGG ATTTGTTCTC CGGACATCAG GCGGAAGAAG TGAGAGTAAT 
TTGTCATCAG TCTGCTATAT ATTTGAATCA AACAATGAGG GAGAAAAGAT TTGTGATTCT 
GTTGGACTGG CAAAACAGAT AGCTTTGCAT GCTGAACTGG ATCGTAGGGC ATCAGAAAAA 
CAAAAAGAAA TAGAGAGAGT AAAAGAGAAA CAGCAGAAAG AGCTCAGCAA ACAGAAACAG 
ATTGAGAAGG ACTTGGAAGA ACAGAGTCGG TTGATAGCTG CCTCCAGTAG ACCAAACCAG 
GCTGGTAGTG AGGGCCAGCT TGTTCTTAGC AGTAGCCAAT CAGAGGAGAG TGATTTGGGA 
GAAGAAGGGA AAAAAAGAGA GTCGGAAGCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103910.1
CDS	538..2130
Translation	MMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNGQLEEFLANIGTSVQNVRREMDGDVETMQQTIEDLEVASDPLYLPDPDPTKFPINRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPSFQQRHESLRPGGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQAGSEGQLVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_030248050.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248050.1

ORF Insert Sequence:

ATGATGCATT ATTTTTGTGC ACTAAATACT CTTCAGTACA AGAAGAAAAT AGCTTTGTTA 
GAACCTCTAC TGGGATACAT GCAGGCTCAG ATAAGTTTCT TTAAGATGGG TTCTGAAAAT 
CTCAATGGAC AACTGGAAGA ATTCTTAGCT AACATTGGAA CAAGTGTACA GAATGTTCGA 
AGAGAAATGG ATGGTGATGT AGAGACCATG CAGCAGACAA TAGAAGACTT GGAGGTAGCC 
AGTGACCCTT TATATCTGCC TGACCCGGAT CCCACCAAGT TTCCCATCAA TCGAAATTTA 
ACCCGAAAGG CTGGATACCT AAATGCTAGG AATAAAACAG GCTTGGTGTC ATCTACCTGG 
GATAGACAGT TTTACTTCAC GCAGGGTGGA AACTTAATGA GTCAAGCCCG TGGAGATGTG 
GCAGGAGGCC TAGCCATGGA CATAGACAAC TGTTCAGTGA TGGCTGTGGA CTGTGAAGAC 
AGACGATACT GTTTTCAGAT CACTTCTTTT GATGGAAAAA AATCTTCAAT ATTGCAAGCA 
GAGAGTAAAA AAGACCATGA AGAGTGGATC TGTACAATAA ACAACATATC TAAACAAATA 
TATTTGAGTG AAAATCCAGA GGAAACTGCT GCACGAGTGA ATCAGTCTGC TCTAGAAGCT 
GTCACTCCTT CCCCATCTTT CCAGCAGAGA CACGAGAGCC TGCGTCCAGG AGGACAATCT 
CGGCCACCAA CAGCTCGAAC CAGCAGTTCA GGATCCTTGG GATCAGAGTC TACAAATTTG 
GCTGCCCTCT CTCTAGATTC TCTTGTTGCT CCGGATACAC CAATACAGTT TGATATAATT 
TCTCCTGTGT GTGAAGATCA GCCTGGCCAG GCAAAAGCCT TTGGTCAAGG AGGCAGGCGT 
ACAAATCCAT TTGGAGAATC TGGAGGAAGC ACAAAATCAG AAACAGAAGA CTCTATTCTT 
CATCAGTTAT TTATTGTCCG ATTCCTTGGT TCTATGGAGG TAAAGTCAGA TGACCATCCA 
GATGTTGTCT ATGAAACAAT GCGCCAGATC TTAGCTGCTC GGGCCATCCA CAACATCTTT 
CGTATGACAG AATCACATTT ATTAGTCACT TGTGACTGCT TGAAGTTAAT TGATCCGCAG 
ACCCAAGTTA CAAGGCTCAC ATTTCCATTG CCGTGTGTAG TTCTGTATGC TACGCACCAG 
GAAAATAAAC GACTTTTTGG ATTTGTTCTC CGGACATCAG GCGGAAGAAG TGAGAGTAAT 
TTGTCATCAG TCTGCTATAT ATTTGAATCA AACAATGAGG GAGAAAAGAT TTGTGATTCT 
GTTGGACTGG CAAAACAGAT AGCTTTGCAT GCTGAACTGG ATCGTAGGGC ATCAGAAAAA 
CAAAAAGAAA TAGAGAGAGT AAAAGAGAAA CAGCAGAAAG AGCTCAGCAA ACAGAAACAG 
ATTGAGAAGG ACTTGGAAGA ACAGAGTCGG TTGATAGCTG CCTCCAGTAG ACCAAACCAG 
GCTGGTAGTG AGGGCCAGCT TGTTCTTAGC AGTAGCCAAT CAGAGGAGAG TGATTTGGGA 
GAAGAAGGGA AAAAAAGAGA GTCGGAAGCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Appl1 ( XM_017316214.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171703.1 Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X2, mRNA.

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CloneID	OMu78869
Clone ID Related Accession (Same CDS sequence)	XM_017316214.2 , XM_017316214.1
Accession Version	XM_017316214.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DCC-interacting protein 13-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316214.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTCC CCATTTCCCA GTTTAAAGAA AGAGATCTGA AAGAAATATT GACACTAAAG 
GAGGTATTTC AAATTGCTAG TAATGATCAT GATGCCGCAA TTAATAGGTA CAGTCGGTTG 
TCAAAAAAGA GAGAAAATGA CAAGGTAAAA TATGAAGTTA CAGAGGATGT ATATACTTCC 
AGGAAAAAAC AACACCAGAC CATGATGCAT TATTTTTGTG CACTAAATAC TCTTCAGTAC 
AAGAAGAAAA TAGCTTTGTT AGAACCTCTA CTGGGATACA TGCAGGCTCA GATAAGTTTC 
TTTAAGATGG GTTCTGAAAA TCTCAATGGA CAACTGGAAG AATTCTTAGC TAACATTGGA 
ACAAGTGTAC AGAATGTTCG AAGAGAAATG GATGGTGATG TAGAGACCAT GCAGCAGACA 
ATAGAAGACT TGGAGGTAGC CAGTGACCCT TTATATCTGC CTGACCCGGA TCCCACCAAG 
TTTCCCATCA ATCGAAATTT AACCCGAAAG GCTGGATACC TAAATGCTAG GAATAAAACA 
GGCTTGGTGT CATCTACCTG GGATAGACAG TTTTACTTCA CGCAGGGTGG AAACTTAATG 
AGTCAAGCCC GTGGAGATGT GGCAGGAGGC CTAGCCATGG ACATAGACAA CTGTTCAGTG 
ATGGCTGTGG ACTGTGAAGA CAGACGATAC TGTTTTCAGA TCACTTCTTT TGATGGAAAA 
AAATCTTCAA TATTGCAAGC AGAGAGTAAA AAAGACCATG AAGAGTGGAT CTGTACAATA 
AACAACATAT CTAAACAAAT ATATTTGAGT GAAAATCCAG AGGAAACTGC TGCACGAGTG 
AATCAGTCTG CTCTAGAAGC TGTCACTCCT TCCCCATCTT TCCAGCAGAG ACACGAGAGC 
CTGCGTCCAG GAGGACAATC TCGGCCACCA ACAGCTCGAA CCAGCAGTTC AGGATCCTTG 
GGATCAGAGT CTACAAATTT GGCTGCCCTC TCTCTAGATT CTCTTGTTGC TCCGGATACA 
CCAATACAGT TTGATATAAT TTCTCCTGTG TGTGAAGATC AGCCTGGCCA GGCAAAAGCC 
TTTGGTCAAG GAGGCAGGCG TACAAATCCA TTTGGAGAAT CTGGAGGAAG CACAAAATCA 
GAAACAGAAG ACTCTATTCT TCATCAGTTA TTTATTGTCC GATTCCTTGG TTCTATGGAG 
GTAAAGTCAG ATGACCATCC AGATGTTGTC TATGAAACAA TGCGCCAGAT CTTAGCTGCT 
CGGGCCATCC ACAACATCTT TCGTATGACA GAATCACATT TATTAGTCAC TTGTGACTGC 
TTGAAGTTAA TTGATCCGCA GACCCAAGTT ACAAGGCTCA CATTTCCATT GCCGTGTGTA 
GTTCTGTATG CTACGCACCA GGAAAATAAA CGACTTTTTG GATTTGTTCT CCGGACATCA 
GGCGGAAGAA GTGAGAGTAA TTTGTCATCA GTCTGCTATA TATTTGAATC AAACAATGAG 
GGAGAAAAGA TTTGTGATTC TGTTGGACTG GCAAAACAGA TAGCTTTGCA TGCTGAACTG 
GATCGTAGGG CATCAGAAAA ACAAAAAGAA ATAGAGAGAG TAAAAGAGAA ACAGCAGAAA 
GAGCTCAGCA AACAGAAACA GATTGAGAAG GACTTGGAAG AACAGAGTCG GTTGATAGCT 
GCCTCCAGTA GACCAAACCA GGCTGGTAGT GAGGGCCAGC TTGTTCTTAG CAGTAGCCAA 
TCAGAGGAGA GTGATTTGGG AGAAGAAGGG AAAAAAAGAG AGTCGGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171703.1
CDS	519..2312
Translation	MMFPISQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNGQLEEFLANIGTSVQNVRREMDGDVETMQQTIEDLEVASDPLYLPDPDPTKFPINRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPSFQQRHESLRPGGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQAGSEGQLVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_017316214.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316214.2

ORF Insert Sequence:

ATGATGTTCC CCATTTCCCA GTTTAAAGAA AGAGATCTGA AAGAAATATT GACACTAAAG 
GAGGTATTTC AAATTGCTAG TAATGATCAT GATGCCGCAA TTAATAGGTA CAGTCGGTTG 
TCAAAAAAGA GAGAAAATGA CAAGGTAAAA TATGAAGTTA CAGAGGATGT ATATACTTCC 
AGGAAAAAAC AACACCAGAC CATGATGCAT TATTTTTGTG CACTAAATAC TCTTCAGTAC 
AAGAAGAAAA TAGCTTTGTT AGAACCTCTA CTGGGATACA TGCAGGCTCA GATAAGTTTC 
TTTAAGATGG GTTCTGAAAA TCTCAATGGA CAACTGGAAG AATTCTTAGC TAACATTGGA 
ACAAGTGTAC AGAATGTTCG AAGAGAAATG GATGGTGATG TAGAGACCAT GCAGCAGACA 
ATAGAAGACT TGGAGGTAGC CAGTGACCCT TTATATCTGC CTGACCCGGA TCCCACCAAG 
TTTCCCATCA ATCGAAATTT AACCCGAAAG GCTGGATACC TAAATGCTAG GAATAAAACA 
GGCTTGGTGT CATCTACCTG GGATAGACAG TTTTACTTCA CGCAGGGTGG AAACTTAATG 
AGTCAAGCCC GTGGAGATGT GGCAGGAGGC CTAGCCATGG ACATAGACAA CTGTTCAGTG 
ATGGCTGTGG ACTGTGAAGA CAGACGATAC TGTTTTCAGA TCACTTCTTT TGATGGAAAA 
AAATCTTCAA TATTGCAAGC AGAGAGTAAA AAAGACCATG AAGAGTGGAT CTGTACAATA 
AACAACATAT CTAAACAAAT ATATTTGAGT GAAAATCCAG AGGAAACTGC TGCACGAGTG 
AATCAGTCTG CTCTAGAAGC TGTCACTCCT TCCCCATCTT TCCAGCAGAG ACACGAGAGC 
CTGCGTCCAG GAGGACAATC TCGGCCACCA ACAGCTCGAA CCAGCAGTTC AGGATCCTTG 
GGATCAGAGT CTACAAATTT GGCTGCCCTC TCTCTAGATT CTCTTGTTGC TCCGGATACA 
CCAATACAGT TTGATATAAT TTCTCCTGTG TGTGAAGATC AGCCTGGCCA GGCAAAAGCC 
TTTGGTCAAG GAGGCAGGCG TACAAATCCA TTTGGAGAAT CTGGAGGAAG CACAAAATCA 
GAAACAGAAG ACTCTATTCT TCATCAGTTA TTTATTGTCC GATTCCTTGG TTCTATGGAG 
GTAAAGTCAG ATGACCATCC AGATGTTGTC TATGAAACAA TGCGCCAGAT CTTAGCTGCT 
CGGGCCATCC ACAACATCTT TCGTATGACA GAATCACATT TATTAGTCAC TTGTGACTGC 
TTGAAGTTAA TTGATCCGCA GACCCAAGTT ACAAGGCTCA CATTTCCATT GCCGTGTGTA 
GTTCTGTATG CTACGCACCA GGAAAATAAA CGACTTTTTG GATTTGTTCT CCGGACATCA 
GGCGGAAGAA GTGAGAGTAA TTTGTCATCA GTCTGCTATA TATTTGAATC AAACAATGAG 
GGAGAAAAGA TTTGTGATTC TGTTGGACTG GCAAAACAGA TAGCTTTGCA TGCTGAACTG 
GATCGTAGGG CATCAGAAAA ACAAAAAGAA ATAGAGAGAG TAAAAGAGAA ACAGCAGAAA 
GAGCTCAGCA AACAGAAACA GATTGAGAAG GACTTGGAAG AACAGAGTCG GTTGATAGCT 
GCCTCCAGTA GACCAAACCA GGCTGGTAGT GAGGGCCAGC TTGTTCTTAG CAGTAGCCAA 
TCAGAGGAGA GTGATTTGGG AGAAGAAGGG AAAAAAAGAG AGTCGGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

APPL1 prevents pancreatic beta cell death and inflammation by dampening NF?B activation in a mouse model of type 1 diabetes.
Diabetologia60(3)464-474(2017 Mar)
Jiang X,Zhou Y,Wu KK,Chen Z,Xu A,Cheng KK

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Appl1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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