rgs12a gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009303085.2	XP_009301360.1	regulator of G-protein signaling 12 isoform X2
XM_005172219.3	XP_005172276.1	regulator of G-protein signaling 12 isoform X5
XM_694005.7	XP_699097.4	regulator of G-protein signaling 12 isoform X1
XM_009303087.2	XP_009301362.1	regulator of G-protein signaling 12 isoform X4
XM_694005.8	XP_699097.4	regulator of G-protein signaling 12 isoform X1
XM_009303086.2	XP_009301361.1	regulator of G-protein signaling 12 isoform X3
XM_009303085.3	XP_009301360.1	regulator of G-protein signaling 12 isoform X2
XM_009303086.3	XP_009301361.1	regulator of G-protein signaling 12 isoform X3
XM_009303087.3	XP_009301362.1	regulator of G-protein signaling 12 isoform X4
XM_005172219.4	XP_005172276.1	regulator of G-protein signaling 12 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa12810	XM_005172219.4 Latest version!	Danio rerio regulator of G protein signaling 12a (rgs12a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa45981	XM_009303087.3 Latest version!	Danio rerio regulator of G protein signaling 12a (rgs12a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa12809	XM_694005.8 Latest version!	Danio rerio regulator of G protein signaling 12a (rgs12a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa45979	XM_009303085.3 Latest version!	Danio rerio regulator of G protein signaling 12a (rgs12a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa45980	XM_009303086.3 Latest version!	Danio rerio regulator of G protein signaling 12a (rgs12a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa12809	XM_694005.7	Danio rerio regulator of G-protein signaling 12a (rgs12a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa45979	XM_009303085.2	Danio rerio regulator of G-protein signaling 12a (rgs12a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa45980	XM_009303086.2	Danio rerio regulator of G-protein signaling 12a (rgs12a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa45981	XM_009303087.2	Danio rerio regulator of G-protein signaling 12a (rgs12a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa12810	XM_005172219.3	Danio rerio regulator of G-protein signaling 12a (rgs12a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

rgs12a ( XM_005172219.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172276.1 Danio rerio regulator of G-protein signaling 12a (rgs12a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa12810
Clone ID Related Accession (Same CDS sequence)	XM_005172219.3 , XM_005172219.4
Accession Version	XM_005172219.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	regulator of G-protein signaling 12 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172219.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAA CTTTAACTCT TCGCTCTTCT TCGGCTGCAT CAGATGGAGA GCGCAGTGGT 
GGTGGTGTCC AGCGTCACGA CTGCAGCAGC GTGAGCAGTT TGGACAGTAA CGGTAGTCTG 
CCCAGCGTCA CCAACCACTG TCGTTTCACA GAGCGCCGCG TTGCATTATG GGCAGTCAGC 
TTCGAGCGAT TGCTTCAGGA TCCTGTGGGT GTCCGCTATT TTTCTGAGTT TCTAAAGAAG 
GAGTTTAGCG AGGAAAACAT CTTATTCTGG CAGGCCTGTG AATGCTTCAG CCAAGTTCCA 
GAACATGACA AAAAGCAGCT GTCCCAGAAG GCCAAAGAGA TCTACAACCG TTTCCTCTCA 
AGTAAAGCCA CAATGCCAGT GAACATCGAC AGTCAAGCTC AACTTGCGGA CGATGTCCTG 
ACAGCTCCAC AGCCTAACAT GTTCAAACAG CCACAGCTAC AGATCTTCAA CCTGATGAAG 
ATGGACAGTT ATGCGCGCTT CTTGAAGTCC ACTCTCTATC AGGAGTGTAT GCTGGCAGAG 
GTGGAGGGTC GACCTCTGCC TGACCCCTGT CCCATCCCGT GCAGCCCTGC CCCATCCAAA 
CACAGCTTCA CCTCTGACCG CTCCACATTC TCCACTCCTA AAAAGGAAAA CAGGAAGCCC 
AAAACAGGCA GGTCAAGCGA TGATCTCAGA GAGAAACACT CTGATAAGAA AAACGGCTTC 
TTTTCCTGGT ACAGATATCC AAGTATTGGG AAAGGACAAA AGAAAAGAGA GGCCCCAGAT 
TTACCCTACC ACTGTAATGG GCGGAGAGAG TCTCAAGGTT CTCTGTCTTC TGGAGCGAGT 
CAAGAGCTCA ACACTTCCTG TTCAGGAGGA AAGAATGAGC AGTGTGAGCT AAGAGGTTCG 
AGTTGTGCTT TGGATAAGGA GCGAGAGCGC TGCAGCAGGA CATGTACAGT AACACTGCCC 
GACGGCTCCA GCTGCTCTAT TCCTCTCCGA CAGGGAGCCT CCATCAGACA AGTGCTGCTA 
GAGCTCTGCC AAAAACAACA CATCAACCTG GCTGCTGTCG ACCTCTTTCT CACCGGTGGA 
GAGAAGCCTT TGGTCTTGGA TCAAGATAGT ATTACTCTCA GCTCCAGGGA ACTTCGTCTT 
GAGAAGCGCA CTTTGTTCAG ATTGGACCTG GTACCCATTA ACAGATCGGT TGGGTTGAAA 
GCGAAGCCCA CAAAACCTGT GACTGAGGTT CTACGGCCTG TGGTTGCCAA GTATGGACTC 
CAGCTTGGCG ATTTGGTGGC CAGAATAAGT GGCGAGACAG AGGTACTCGA TCTTGGAGTG 
CCCATATCAA ACCTTGATGG CCTGCGAGTT GTCTTGGAGA GGGCAGATCC AGCCAAAGCA 
GAAAAGTCTA AAACACTCAA CTCCAAAACA CCAGCTGCCA AGAAAGGGTT TCCAAGAGAG 
AGAGACGATA ACTCGACAGT GAAACCATTG AAAGCAGATG TTGAAGACAA GCATCCCCGC 
ACAGCTGCGT CTGAGAGGAA GAAAGGCAAA AAGAATCATA TAGATGAAAC TGAAGAGTTT 
TTCGAGTTGT TGAGTCGTGC TCAGAGCAGT CGTGTTGACG ACCAGAGAGG CCTGCTGAGA 
AAAGAAGATC TTGTCCTTCC CGACTTCCTT CGAGTAAACC CTGATCCCAA ACCTCAATCT 
CCTCCGTCCT GTTCAACCCC GACCTCTCAT AAACCCGGCC ACACCACTAC CACCTCCACA 
AACCCCCATC CCCCTAAACC CAGCCCCTGT AACGGACACC AAGCCTCAGC CCCGTTCAGC 
GCGCCACTCT CCCCCATCCT GCGTTCTGCT GGTGCCCGCG AGCAGGAGGA GGAGGAAGGC 
CTAGGAGATT TGACTCTGGT GGCGGAGGGT GACATCAGCA GCCCAAACTC CACCCTGCTC 
CCGCCTCCAC CGCCCGTATC ACCACCGCCT TATGATGGTA GTCTAGCAGA GGCCAACTTT 
ACTCCTCCAC CGGCTGTTCA CGGCACAGGT ACAGAAGAAG AAAACACGAG CACTTCAGCA 
GTAAACAGCT CAACAGCACA GCCGGACTTG CGTGTGCGGG ACAAGGGTGT CACTCTGGAG 
GACAGTTTTG AAGGATACGC AGCTGAACTT CGCCAGTGCC AGAACAAAAT GAGAAACGGC 
GTTTATCCCT CCACAGAAGC CCCCCGTCCC CTCAGCGGTG TGCTGGAAGT CAACGAGAGC 
GACACTGTGC AATACAAAGC AACGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172276.1
CDS	362..2614
Translation	MEPTLTLRSSSAASDGERSGGGVQRHDCSSVSSLDSNGSLPSVTNHCRFTERRVALWAVSFERLLQDPVGVRYFSEFLKKEFSEENILFWQACECFSQVPEHDKKQLSQKAKEIYNRFLSSKATMPVNIDSQAQLADDVLTAPQPNMFKQPQLQIFNLMKMDSYARFLKSTLYQECMLAEVEGRPLPDPCPIPCSPAPSKHSFTSDRSTFSTPKKENRKPKTGRSSDDLREKHSDKKNGFFSWYRYPSIGKGQKKREAPDLPYHCNGRRESQGSLSSGASQELNTSCSGGKNEQCELRGSSCALDKERERCSRTCTVTLPDGSSCSIPLRQGASIRQVLLELCQKQHINLAAVDLFLTGGEKPLVLDQDSITLSSRELRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLQLGDLVARISGETEVLDLGVPISNLDGLRVVLERADPAKAEKSKTLNSKTPAAKKGFPRERDDNSTVKPLKADVEDKHPRTAASERKKGKKNHIDETEEFFELLSRAQSSRVDDQRGLLRKEDLVLPDFLRVNPDPKPQSPPSCSTPTSHKPGHTTTTSTNPHPPKPSPCNGHQASAPFSAPLSPILRSAGAREQEEEEGLGDLTLVAEGDISSPNSTLLPPPPPVSPPPYDGSLAEANFTPPPAVHGTGTEEENTSTSAVNSSTAQPDLRVRDKGVTLEDSFEGYAAELRQCQNKMRNGVYPSTEAPRPLSGVLEVNESDTVQYKATFV

Target ORF information:

RefSeq Version	XM_005172219.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio regulator of G-protein signaling 12a (rgs12a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172219.3

ORF Insert Sequence:

ATGGAGCCAA CTTTAACTCT TCGCTCTTCT TCGGCTGCAT CAGATGGAGA GCGCAGTGGT 
GGTGGTGTCC AGCGTCACGA CTGCAGCAGC GTGAGCAGTT TGGACAGTAA CGGTAGTCTG 
CCCAGCGTCA CCAACCACTG TCGTTTCACA GAGCGCCGCG TTGCATTATG GGCAGTCAGC 
TTCGAGCGAT TGCTTCAGGA TCCTGTGGGT GTCCGCTATT TTTCTGAGTT TCTAAAGAAG 
GAGTTTAGCG AGGAAAACAT CTTATTCTGG CAGGCCTGTG AATGCTTCAG CCAAGTTCCA 
GAACATGACA AAAAGCAGCT GTCCCAGAAG GCCAAAGAGA TCTACAACCG TTTCCTCTCA 
AGTAAAGCCA CAATGCCAGT GAACATCGAC AGTCAAGCTC AACTTGCGGA CGATGTCCTG 
ACAGCTCCAC AGCCTAACAT GTTCAAACAG CCACAGCTAC AGATCTTCAA CCTGATGAAG 
ATGGACAGTT ATGCGCGCTT CTTGAAGTCC ACTCTCTATC AGGAGTGTAT GCTGGCAGAG 
GTGGAGGGTC GACCTCTGCC TGACCCCTGT CCCATCCCGT GCAGCCCTGC CCCATCCAAA 
CACAGCTTCA CCTCTGACCG CTCCACATTC TCCACTCCTA AAAAGGAAAA CAGGAAGCCC 
AAAACAGGCA GGTCAAGCGA TGATCTCAGA GAGAAACACT CTGATAAGAA AAACGGCTTC 
TTTTCCTGGT ACAGATATCC AAGTATTGGG AAAGGACAAA AGAAAAGAGA GGCCCCAGAT 
TTACCCTACC ACTGTAATGG GCGGAGAGAG TCTCAAGGTT CTCTGTCTTC TGGAGCGAGT 
CAAGAGCTCA ACACTTCCTG TTCAGGAGGA AAGAATGAGC AGTGTGAGCT AAGAGGTTCG 
AGTTGTGCTT TGGATAAGGA GCGAGAGCGC TGCAGCAGGA CATGTACAGT AACACTGCCC 
GACGGCTCCA GCTGCTCTAT TCCTCTCCGA CAGGGAGCCT CCATCAGACA AGTGCTGCTA 
GAGCTCTGCC AAAAACAACA CATCAACCTG GCTGCTGTCG ACCTCTTTCT CACCGGTGGA 
GAGAAGCCTT TGGTCTTGGA TCAAGATAGT ATTACTCTCA GCTCCAGGGA ACTTCGTCTT 
GAGAAGCGCA CTTTGTTCAG ATTGGACCTG GTACCCATTA ACAGATCGGT TGGGTTGAAA 
GCGAAGCCCA CAAAACCTGT GACTGAGGTT CTACGGCCTG TGGTTGCCAA GTATGGACTC 
CAGCTTGGCG ATTTGGTGGC CAGAATAAGT GGCGAGACAG AGGTACTCGA TCTTGGAGTG 
CCCATATCAA ACCTTGATGG CCTGCGAGTT GTCTTGGAGA GGGCAGATCC AGCCAAAGCA 
GAAAAGTCTA AAACACTCAA CTCCAAAACA CCAGCTGCCA AGAAAGGGTT TCCAAGAGAG 
AGAGACGATA ACTCGACAGT GAAACCATTG AAAGCAGATG TTGAAGACAA GCATCCCCGC 
ACAGCTGCGT CTGAGAGGAA GAAAGGCAAA AAGAATCATA TAGATGAAAC TGAAGAGTTT 
TTCGAGTTGT TGAGTCGTGC TCAGAGCAGT CGTGTTGACG ACCAGAGAGG CCTGCTGAGA 
AAAGAAGATC TTGTCCTTCC CGACTTCCTT CGAGTAAACC CTGATCCCAA ACCTCAATCT 
CCTCCGTCCT GTTCAACCCC GACCTCTCAT AAACCCGGCC ACACCACTAC CACCTCCACA 
AACCCCCATC CCCCTAAACC CAGCCCCTGT AACGGACACC AAGCCTCAGC CCCGTTCAGC 
GCGCCACTCT CCCCCATCCT GCGTTCTGCT GGTGCCCGCG AGCAGGAGGA GGAGGAAGGC 
CTAGGAGATT TGACTCTGGT GGCGGAGGGT GACATCAGCA GCCCAAACTC CACCCTGCTC 
CCGCCTCCAC CGCCCGTATC ACCACCGCCT TATGATGGTA GTCTAGCAGA GGCCAACTTT 
ACTCCTCCAC CGGCTGTTCA CGGCACAGGT ACAGAAGAAG AAAACACGAG CACTTCAGCA 
GTAAACAGCT CAACAGCACA GCCGGACTTG CGTGTGCGGG ACAAGGGTGT CACTCTGGAG 
GACAGTTTTG AAGGATACGC AGCTGAACTT CGCCAGTGCC AGAACAAAAT GAGAAACGGC 
GTTTATCCCT CCACAGAAGC CCCCCGTCCC CTCAGCGGTG TGCTGGAAGT CAACGAGAGC 
GACACTGTGC AATACAAAGC AACGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rgs12a ( XM_005172219.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172276.1 Danio rerio regulator of G protein signaling 12a (rgs12a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa12810
Clone ID Related Accession (Same CDS sequence)	XM_005172219.3 , XM_005172219.4
Accession Version	XM_005172219.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	regulator of G-protein signaling 12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172219.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAA CTTTAACTCT TCGCTCTTCT TCGGCTGCAT CAGATGGAGA GCGCAGTGGT 
GGTGGTGTCC AGCGTCACGA CTGCAGCAGC GTGAGCAGTT TGGACAGTAA CGGTAGTCTG 
CCCAGCGTCA CCAACCACTG TCGTTTCACA GAGCGCCGCG TTGCATTATG GGCAGTCAGC 
TTCGAGCGAT TGCTTCAGGA TCCTGTGGGT GTCCGCTATT TTTCTGAGTT TCTAAAGAAG 
GAGTTTAGCG AGGAAAACAT CTTATTCTGG CAGGCCTGTG AATGCTTCAG CCAAGTTCCA 
GAACATGACA AAAAGCAGCT GTCCCAGAAG GCCAAAGAGA TCTACAACCG TTTCCTCTCA 
AGTAAAGCCA CAATGCCAGT GAACATCGAC AGTCAAGCTC AACTTGCGGA CGATGTCCTG 
ACAGCTCCAC AGCCTAACAT GTTCAAACAG CCACAGCTAC AGATCTTCAA CCTGATGAAG 
ATGGACAGTT ATGCGCGCTT CTTGAAGTCC ACTCTCTATC AGGAGTGTAT GCTGGCAGAG 
GTGGAGGGTC GACCTCTGCC TGACCCCTGT CCCATCCCGT GCAGCCCTGC CCCATCCAAA 
CACAGCTTCA CCTCTGACCG CTCCACATTC TCCACTCCTA AAAAGGAAAA CAGGAAGCCC 
AAAACAGGCA GGTCAAGCGA TGATCTCAGA GAGAAACACT CTGATAAGAA AAACGGCTTC 
TTTTCCTGGT ACAGATATCC AAGTATTGGG AAAGGACAAA AGAAAAGAGA GGCCCCAGAT 
TTACCCTACC ACTGTAATGG GCGGAGAGAG TCTCAAGGTT CTCTGTCTTC TGGAGCGAGT 
CAAGAGCTCA ACACTTCCTG TTCAGGAGGA AAGAATGAGC AGTGTGAGCT AAGAGGTTCG 
AGTTGTGCTT TGGATAAGGA GCGAGAGCGC TGCAGCAGGA CATGTACAGT AACACTGCCC 
GACGGCTCCA GCTGCTCTAT TCCTCTCCGA CAGGGAGCCT CCATCAGACA AGTGCTGCTA 
GAGCTCTGCC AAAAACAACA CATCAACCTG GCTGCTGTCG ACCTCTTTCT CACCGGTGGA 
GAGAAGCCTT TGGTCTTGGA TCAAGATAGT ATTACTCTCA GCTCCAGGGA ACTTCGTCTT 
GAGAAGCGCA CTTTGTTCAG ATTGGACCTG GTACCCATTA ACAGATCGGT TGGGTTGAAA 
GCGAAGCCCA CAAAACCTGT GACTGAGGTT CTACGGCCTG TGGTTGCCAA GTATGGACTC 
CAGCTTGGCG ATTTGGTGGC CAGAATAAGT GGCGAGACAG AGGTACTCGA TCTTGGAGTG 
CCCATATCAA ACCTTGATGG CCTGCGAGTT GTCTTGGAGA GGGCAGATCC AGCCAAAGCA 
GAAAAGTCTA AAACACTCAA CTCCAAAACA CCAGCTGCCA AGAAAGGGTT TCCAAGAGAG 
AGAGACGATA ACTCGACAGT GAAACCATTG AAAGCAGATG TTGAAGACAA GCATCCCCGC 
ACAGCTGCGT CTGAGAGGAA GAAAGGCAAA AAGAATCATA TAGATGAAAC TGAAGAGTTT 
TTCGAGTTGT TGAGTCGTGC TCAGAGCAGT CGTGTTGACG ACCAGAGAGG CCTGCTGAGA 
AAAGAAGATC TTGTCCTTCC CGACTTCCTT CGAGTAAACC CTGATCCCAA ACCTCAATCT 
CCTCCGTCCT GTTCAACCCC GACCTCTCAT AAACCCGGCC ACACCACTAC CACCTCCACA 
AACCCCCATC CCCCTAAACC CAGCCCCTGT AACGGACACC AAGCCTCAGC CCCGTTCAGC 
GCGCCACTCT CCCCCATCCT GCGTTCTGCT GGTGCCCGCG AGCAGGAGGA GGAGGAAGGC 
CTAGGAGATT TGACTCTGGT GGCGGAGGGT GACATCAGCA GCCCAAACTC CACCCTGCTC 
CCGCCTCCAC CGCCCGTATC ACCACCGCCT TATGATGGTA GTCTAGCAGA GGCCAACTTT 
ACTCCTCCAC CGGCTGTTCA CGGCACAGGT ACAGAAGAAG AAAACACGAG CACTTCAGCA 
GTAAACAGCT CAACAGCACA GCCGGACTTG CGTGTGCGGG ACAAGGGTGT CACTCTGGAG 
GACAGTTTTG AAGGATACGC AGCTGAACTT CGCCAGTGCC AGAACAAAAT GAGAAACGGC 
GTTTATCCCT CCACAGAAGC CCCCCGTCCC CTCAGCGGTG TGCTGGAAGT CAACGAGAGC 
GACACTGTGC AATACAAAGC AACGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172276.1
CDS	518..2770
Translation	MEPTLTLRSSSAASDGERSGGGVQRHDCSSVSSLDSNGSLPSVTNHCRFTERRVALWAVSFERLLQDPVGVRYFSEFLKKEFSEENILFWQACECFSQVPEHDKKQLSQKAKEIYNRFLSSKATMPVNIDSQAQLADDVLTAPQPNMFKQPQLQIFNLMKMDSYARFLKSTLYQECMLAEVEGRPLPDPCPIPCSPAPSKHSFTSDRSTFSTPKKENRKPKTGRSSDDLREKHSDKKNGFFSWYRYPSIGKGQKKREAPDLPYHCNGRRESQGSLSSGASQELNTSCSGGKNEQCELRGSSCALDKERERCSRTCTVTLPDGSSCSIPLRQGASIRQVLLELCQKQHINLAAVDLFLTGGEKPLVLDQDSITLSSRELRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLQLGDLVARISGETEVLDLGVPISNLDGLRVVLERADPAKAEKSKTLNSKTPAAKKGFPRERDDNSTVKPLKADVEDKHPRTAASERKKGKKNHIDETEEFFELLSRAQSSRVDDQRGLLRKEDLVLPDFLRVNPDPKPQSPPSCSTPTSHKPGHTTTTSTNPHPPKPSPCNGHQASAPFSAPLSPILRSAGAREQEEEEGLGDLTLVAEGDISSPNSTLLPPPPPVSPPPYDGSLAEANFTPPPAVHGTGTEEENTSTSAVNSSTAQPDLRVRDKGVTLEDSFEGYAAELRQCQNKMRNGVYPSTEAPRPLSGVLEVNESDTVQYKATFV

Target ORF information:

RefSeq Version	XM_005172219.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio regulator of G protein signaling 12a (rgs12a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172219.4

ORF Insert Sequence:

ATGGAGCCAA CTTTAACTCT TCGCTCTTCT TCGGCTGCAT CAGATGGAGA GCGCAGTGGT 
GGTGGTGTCC AGCGTCACGA CTGCAGCAGC GTGAGCAGTT TGGACAGTAA CGGTAGTCTG 
CCCAGCGTCA CCAACCACTG TCGTTTCACA GAGCGCCGCG TTGCATTATG GGCAGTCAGC 
TTCGAGCGAT TGCTTCAGGA TCCTGTGGGT GTCCGCTATT TTTCTGAGTT TCTAAAGAAG 
GAGTTTAGCG AGGAAAACAT CTTATTCTGG CAGGCCTGTG AATGCTTCAG CCAAGTTCCA 
GAACATGACA AAAAGCAGCT GTCCCAGAAG GCCAAAGAGA TCTACAACCG TTTCCTCTCA 
AGTAAAGCCA CAATGCCAGT GAACATCGAC AGTCAAGCTC AACTTGCGGA CGATGTCCTG 
ACAGCTCCAC AGCCTAACAT GTTCAAACAG CCACAGCTAC AGATCTTCAA CCTGATGAAG 
ATGGACAGTT ATGCGCGCTT CTTGAAGTCC ACTCTCTATC AGGAGTGTAT GCTGGCAGAG 
GTGGAGGGTC GACCTCTGCC TGACCCCTGT CCCATCCCGT GCAGCCCTGC CCCATCCAAA 
CACAGCTTCA CCTCTGACCG CTCCACATTC TCCACTCCTA AAAAGGAAAA CAGGAAGCCC 
AAAACAGGCA GGTCAAGCGA TGATCTCAGA GAGAAACACT CTGATAAGAA AAACGGCTTC 
TTTTCCTGGT ACAGATATCC AAGTATTGGG AAAGGACAAA AGAAAAGAGA GGCCCCAGAT 
TTACCCTACC ACTGTAATGG GCGGAGAGAG TCTCAAGGTT CTCTGTCTTC TGGAGCGAGT 
CAAGAGCTCA ACACTTCCTG TTCAGGAGGA AAGAATGAGC AGTGTGAGCT AAGAGGTTCG 
AGTTGTGCTT TGGATAAGGA GCGAGAGCGC TGCAGCAGGA CATGTACAGT AACACTGCCC 
GACGGCTCCA GCTGCTCTAT TCCTCTCCGA CAGGGAGCCT CCATCAGACA AGTGCTGCTA 
GAGCTCTGCC AAAAACAACA CATCAACCTG GCTGCTGTCG ACCTCTTTCT CACCGGTGGA 
GAGAAGCCTT TGGTCTTGGA TCAAGATAGT ATTACTCTCA GCTCCAGGGA ACTTCGTCTT 
GAGAAGCGCA CTTTGTTCAG ATTGGACCTG GTACCCATTA ACAGATCGGT TGGGTTGAAA 
GCGAAGCCCA CAAAACCTGT GACTGAGGTT CTACGGCCTG TGGTTGCCAA GTATGGACTC 
CAGCTTGGCG ATTTGGTGGC CAGAATAAGT GGCGAGACAG AGGTACTCGA TCTTGGAGTG 
CCCATATCAA ACCTTGATGG CCTGCGAGTT GTCTTGGAGA GGGCAGATCC AGCCAAAGCA 
GAAAAGTCTA AAACACTCAA CTCCAAAACA CCAGCTGCCA AGAAAGGGTT TCCAAGAGAG 
AGAGACGATA ACTCGACAGT GAAACCATTG AAAGCAGATG TTGAAGACAA GCATCCCCGC 
ACAGCTGCGT CTGAGAGGAA GAAAGGCAAA AAGAATCATA TAGATGAAAC TGAAGAGTTT 
TTCGAGTTGT TGAGTCGTGC TCAGAGCAGT CGTGTTGACG ACCAGAGAGG CCTGCTGAGA 
AAAGAAGATC TTGTCCTTCC CGACTTCCTT CGAGTAAACC CTGATCCCAA ACCTCAATCT 
CCTCCGTCCT GTTCAACCCC GACCTCTCAT AAACCCGGCC ACACCACTAC CACCTCCACA 
AACCCCCATC CCCCTAAACC CAGCCCCTGT AACGGACACC AAGCCTCAGC CCCGTTCAGC 
GCGCCACTCT CCCCCATCCT GCGTTCTGCT GGTGCCCGCG AGCAGGAGGA GGAGGAAGGC 
CTAGGAGATT TGACTCTGGT GGCGGAGGGT GACATCAGCA GCCCAAACTC CACCCTGCTC 
CCGCCTCCAC CGCCCGTATC ACCACCGCCT TATGATGGTA GTCTAGCAGA GGCCAACTTT 
ACTCCTCCAC CGGCTGTTCA CGGCACAGGT ACAGAAGAAG AAAACACGAG CACTTCAGCA 
GTAAACAGCT CAACAGCACA GCCGGACTTG CGTGTGCGGG ACAAGGGTGT CACTCTGGAG 
GACAGTTTTG AAGGATACGC AGCTGAACTT CGCCAGTGCC AGAACAAAAT GAGAAACGGC 
GTTTATCCCT CCACAGAAGC CCCCCGTCCC CTCAGCGGTG TGCTGGAAGT CAACGAGAGC 
GACACTGTGC AATACAAAGC AACGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rgs12a ( XM_009303087.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301362.1 Danio rerio regulator of G-protein signaling 12a (rgs12a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa45981
Clone ID Related Accession (Same CDS sequence)	XM_009303087.2 , XM_009303087.3
Accession Version	XM_009303087.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	regulator of G-protein signaling 12 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303087.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCAA AATCAAAGAC TAAGAGAAGA AGCCTCTTGA CTATAAGTCT CATGAGGAGA 
CTTTCCTTCA GAAAGCGGCC CCCGAATCTG TCGGCTGCAT CAGATGGAGA GCGCAGTGGT 
GGTGGTGTCC AGCGTCACGA CTGCAGCAGC GTGAGCAGTT TGGACAGTAA CGGTAGTCTG 
CCCAGCGTCA CCAACCACTG TCGTTTCACA GAGCGCCGCG TTGCATTATG GGCAGTCAGC 
TTCGAGCGAT TGCTTCAGGA TCCTGTGGGT GTCCGCTATT TTTCTGAGTT TCTAAAGAAG 
GAGTTTAGCG AGGAAAACAT CTTATTCTGG CAGGCCTGTG AATGCTTCAG CCAAGTTCCA 
GAACATGACA AAAAGCAGCT GTCCCAGAAG GCCAAAGAGA TCTACAACCG TTTCCTCTCA 
AGTAAAGCCA CAATGCCAGT GAACATCGAC AGTCAAGCTC AACTTGCGGA CGATGTCCTG 
ACAGCTCCAC AGCCTAACAT GTTCAAACAG CCACAGCTAC AGATCTTCAA CCTGATGAAG 
ATGGACAGTT ATGCGCGCTT CTTGAAGTCC ACTCTCTATC AGGAGTGTAT GCTGGCAGAG 
GTGGAGGGTC GACCTCTGCC TGACCCCTGT CCCATCCCGT GCAGCCCTGC CCCATCCAAA 
CACAGCTTCA CCTCTGACCG CTCCACATTC TCCACTCCTA AAAAGGAAAA CAGGAAGCCC 
AAAACAGGCA GGTCAAGCGA TGATCTCAGA GAGAAACACT CTGATAAGAA AAACGGCTTC 
TTTTCCTGGT ACAGATATCC AAGTATTGGG AAAGGACAAA AGAAAAGAGA GGCCCCAGAT 
TTACCCTACC ACTGTAATGG GCGGAGAGAG TCTCAAGGTT CTCTGTCTTC TGGAGCGAGT 
CAAGAGCTCA ACACTTCCTG TTCAGGAGGA AAGAATGAGC AGTGTGAGCT AAGAGGTTCG 
AGTTGTGCTT TGGATAAGGA GCGAGAGCGC TGCAGCAGGA CATGTACAGT AACACTGCCC 
GACGGCTCCA GCTGCTCTAT TCCTCTCCGA CAGGGAGCCT CCATCAGACA AGTGCTGCTA 
GAGCTCTGCC AAAAACAACA CATCAACCTG GCTGCTGTCG ACCTCTTTCT CACCGGTGGA 
GAGAAGCCTT TGGTCTTGGA TCAAGATAGT ATTACTCTCA GCTCCAGGGA ACTTCGTCTT 
GAGAAGCGCA CTTTGTTCAG ATTGGACCTG GTACCCATTA ACAGATCGGT TGGGTTGAAA 
GCGAAGCCCA CAAAACCTGT GACTGAGGTT CTACGGCCTG TGGTTGCCAA GTATGGACTC 
CAGCTTGGCG ATTTGGTGGC CAGAATAAGT GGCGAGACAG AGGTACTCGA TCTTGGAGTG 
CCCATATCAA ACCTTGATGG CCTGCGAGTT GTCTTGGAGA GGGCAGATCC AGCCAAAGCA 
GAAAAGTCTA AAACACTCAA CTCCAAAACA CCAGCTGCCA AGAAAGGGTT TCCAAGAGAG 
AGAGACGATA ACTCGACAGT GAAACCATTG AAAGCAGATG TTGAAGACAA GCATCCCCGC 
ACAGCTGCGT CTGAGAGGAA GAAAGGCAAA AAGAATCATA TAGATGAAAC TGAAGAGTTT 
TTCGAGTTGT TGAGTCGTGC TCAGAGCAGT CGTGTTGACG ACCAGAGAGG CCTGCTGAGA 
AAAGAAGATC TTGTCCTTCC CGACTTCCTT CGAGTAAACC CTGATCCCAA ACCTCAATCT 
CCTCCGTCCT GTTCAACCCC GACCTCTCAT AAACCCGGCC ACACCACTAC CACCTCCACA 
AACCCCCATC CCCCTAAACC CAGCCCCTGT AACGGACACC AAGCCTCAGC CCCGTTCAGC 
GCGCCACTCT CCCCCATCCT GCGTTCTGCT GGTGCCCGCG AGCAGGAGGA GGAGGAAGGC 
CTAGGAGATT TGACTCTGGT GGCGGAGGGT GACATCAGCA GCCCAAACTC CACCCTGCTC 
CCGCCTCCAC CGCCCGTATC ACCACCGCCT TATGATGGTA GTCTAGCAGA GGCCAACTTT 
ACTCCTCCAC CGGCTGTTCA CGGCACAGGT ACAGAAGAAG AAAACACGAG CACTTCAGCA 
GTAAACAGCT CAACAGCACA GCCGGACTTG CGTGTGCGGG ACAAGGGTGT CACTCTGGAG 
GACAGTTTTG AAGGATACGC AGCTGAACTT CGCCAGTGCC AGAACAAAAT GAGAAACGGC 
GTTTATCCCT CCACAGAAGC CCCCCGTCCC CTCAGCGGTG TGCTGGAAGT CAACGAGAGC 
GACACTGTGC AATACAAAGC AACGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301362.1
CDS	26..2338
Translation	MNPKSKTKRRSLLTISLMRRLSFRKRPPNLSAASDGERSGGGVQRHDCSSVSSLDSNGSLPSVTNHCRFTERRVALWAVSFERLLQDPVGVRYFSEFLKKEFSEENILFWQACECFSQVPEHDKKQLSQKAKEIYNRFLSSKATMPVNIDSQAQLADDVLTAPQPNMFKQPQLQIFNLMKMDSYARFLKSTLYQECMLAEVEGRPLPDPCPIPCSPAPSKHSFTSDRSTFSTPKKENRKPKTGRSSDDLREKHSDKKNGFFSWYRYPSIGKGQKKREAPDLPYHCNGRRESQGSLSSGASQELNTSCSGGKNEQCELRGSSCALDKERERCSRTCTVTLPDGSSCSIPLRQGASIRQVLLELCQKQHINLAAVDLFLTGGEKPLVLDQDSITLSSRELRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLQLGDLVARISGETEVLDLGVPISNLDGLRVVLERADPAKAEKSKTLNSKTPAAKKGFPRERDDNSTVKPLKADVEDKHPRTAASERKKGKKNHIDETEEFFELLSRAQSSRVDDQRGLLRKEDLVLPDFLRVNPDPKPQSPPSCSTPTSHKPGHTTTTSTNPHPPKPSPCNGHQASAPFSAPLSPILRSAGAREQEEEEGLGDLTLVAEGDISSPNSTLLPPPPPVSPPPYDGSLAEANFTPPPAVHGTGTEEENTSTSAVNSSTAQPDLRVRDKGVTLEDSFEGYAAELRQCQNKMRNGVYPSTEAPRPLSGVLEVNESDTVQYKATFV

Target ORF information:

RefSeq Version	XM_009303087.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio regulator of G-protein signaling 12a (rgs12a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303087.2

ORF Insert Sequence:

ATGAATCCAA AATCAAAGAC TAAGAGAAGA AGCCTCTTGA CTATAAGTCT CATGAGGAGA 
CTTTCCTTCA GAAAGCGGCC CCCGAATCTG TCGGCTGCAT CAGATGGAGA GCGCAGTGGT 
GGTGGTGTCC AGCGTCACGA CTGCAGCAGC GTGAGCAGTT TGGACAGTAA CGGTAGTCTG 
CCCAGCGTCA CCAACCACTG TCGTTTCACA GAGCGCCGCG TTGCATTATG GGCAGTCAGC 
TTCGAGCGAT TGCTTCAGGA TCCTGTGGGT GTCCGCTATT TTTCTGAGTT TCTAAAGAAG 
GAGTTTAGCG AGGAAAACAT CTTATTCTGG CAGGCCTGTG AATGCTTCAG CCAAGTTCCA 
GAACATGACA AAAAGCAGCT GTCCCAGAAG GCCAAAGAGA TCTACAACCG TTTCCTCTCA 
AGTAAAGCCA CAATGCCAGT GAACATCGAC AGTCAAGCTC AACTTGCGGA CGATGTCCTG 
ACAGCTCCAC AGCCTAACAT GTTCAAACAG CCACAGCTAC AGATCTTCAA CCTGATGAAG 
ATGGACAGTT ATGCGCGCTT CTTGAAGTCC ACTCTCTATC AGGAGTGTAT GCTGGCAGAG 
GTGGAGGGTC GACCTCTGCC TGACCCCTGT CCCATCCCGT GCAGCCCTGC CCCATCCAAA 
CACAGCTTCA CCTCTGACCG CTCCACATTC TCCACTCCTA AAAAGGAAAA CAGGAAGCCC 
AAAACAGGCA GGTCAAGCGA TGATCTCAGA GAGAAACACT CTGATAAGAA AAACGGCTTC 
TTTTCCTGGT ACAGATATCC AAGTATTGGG AAAGGACAAA AGAAAAGAGA GGCCCCAGAT 
TTACCCTACC ACTGTAATGG GCGGAGAGAG TCTCAAGGTT CTCTGTCTTC TGGAGCGAGT 
CAAGAGCTCA ACACTTCCTG TTCAGGAGGA AAGAATGAGC AGTGTGAGCT AAGAGGTTCG 
AGTTGTGCTT TGGATAAGGA GCGAGAGCGC TGCAGCAGGA CATGTACAGT AACACTGCCC 
GACGGCTCCA GCTGCTCTAT TCCTCTCCGA CAGGGAGCCT CCATCAGACA AGTGCTGCTA 
GAGCTCTGCC AAAAACAACA CATCAACCTG GCTGCTGTCG ACCTCTTTCT CACCGGTGGA 
GAGAAGCCTT TGGTCTTGGA TCAAGATAGT ATTACTCTCA GCTCCAGGGA ACTTCGTCTT 
GAGAAGCGCA CTTTGTTCAG ATTGGACCTG GTACCCATTA ACAGATCGGT TGGGTTGAAA 
GCGAAGCCCA CAAAACCTGT GACTGAGGTT CTACGGCCTG TGGTTGCCAA GTATGGACTC 
CAGCTTGGCG ATTTGGTGGC CAGAATAAGT GGCGAGACAG AGGTACTCGA TCTTGGAGTG 
CCCATATCAA ACCTTGATGG CCTGCGAGTT GTCTTGGAGA GGGCAGATCC AGCCAAAGCA 
GAAAAGTCTA AAACACTCAA CTCCAAAACA CCAGCTGCCA AGAAAGGGTT TCCAAGAGAG 
AGAGACGATA ACTCGACAGT GAAACCATTG AAAGCAGATG TTGAAGACAA GCATCCCCGC 
ACAGCTGCGT CTGAGAGGAA GAAAGGCAAA AAGAATCATA TAGATGAAAC TGAAGAGTTT 
TTCGAGTTGT TGAGTCGTGC TCAGAGCAGT CGTGTTGACG ACCAGAGAGG CCTGCTGAGA 
AAAGAAGATC TTGTCCTTCC CGACTTCCTT CGAGTAAACC CTGATCCCAA ACCTCAATCT 
CCTCCGTCCT GTTCAACCCC GACCTCTCAT AAACCCGGCC ACACCACTAC CACCTCCACA 
AACCCCCATC CCCCTAAACC CAGCCCCTGT AACGGACACC AAGCCTCAGC CCCGTTCAGC 
GCGCCACTCT CCCCCATCCT GCGTTCTGCT GGTGCCCGCG AGCAGGAGGA GGAGGAAGGC 
CTAGGAGATT TGACTCTGGT GGCGGAGGGT GACATCAGCA GCCCAAACTC CACCCTGCTC 
CCGCCTCCAC CGCCCGTATC ACCACCGCCT TATGATGGTA GTCTAGCAGA GGCCAACTTT 
ACTCCTCCAC CGGCTGTTCA CGGCACAGGT ACAGAAGAAG AAAACACGAG CACTTCAGCA 
GTAAACAGCT CAACAGCACA GCCGGACTTG CGTGTGCGGG ACAAGGGTGT CACTCTGGAG 
GACAGTTTTG AAGGATACGC AGCTGAACTT CGCCAGTGCC AGAACAAAAT GAGAAACGGC 
GTTTATCCCT CCACAGAAGC CCCCCGTCCC CTCAGCGGTG TGCTGGAAGT CAACGAGAGC 
GACACTGTGC AATACAAAGC AACGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rgs12a ( XM_009303087.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301362.1 Danio rerio regulator of G protein signaling 12a (rgs12a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa45981
Clone ID Related Accession (Same CDS sequence)	XM_009303087.2 , XM_009303087.3
Accession Version	XM_009303087.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	regulator of G-protein signaling 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303087.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCAA AATCAAAGAC TAAGAGAAGA AGCCTCTTGA CTATAAGTCT CATGAGGAGA 
CTTTCCTTCA GAAAGCGGCC CCCGAATCTG TCGGCTGCAT CAGATGGAGA GCGCAGTGGT 
GGTGGTGTCC AGCGTCACGA CTGCAGCAGC GTGAGCAGTT TGGACAGTAA CGGTAGTCTG 
CCCAGCGTCA CCAACCACTG TCGTTTCACA GAGCGCCGCG TTGCATTATG GGCAGTCAGC 
TTCGAGCGAT TGCTTCAGGA TCCTGTGGGT GTCCGCTATT TTTCTGAGTT TCTAAAGAAG 
GAGTTTAGCG AGGAAAACAT CTTATTCTGG CAGGCCTGTG AATGCTTCAG CCAAGTTCCA 
GAACATGACA AAAAGCAGCT GTCCCAGAAG GCCAAAGAGA TCTACAACCG TTTCCTCTCA 
AGTAAAGCCA CAATGCCAGT GAACATCGAC AGTCAAGCTC AACTTGCGGA CGATGTCCTG 
ACAGCTCCAC AGCCTAACAT GTTCAAACAG CCACAGCTAC AGATCTTCAA CCTGATGAAG 
ATGGACAGTT ATGCGCGCTT CTTGAAGTCC ACTCTCTATC AGGAGTGTAT GCTGGCAGAG 
GTGGAGGGTC GACCTCTGCC TGACCCCTGT CCCATCCCGT GCAGCCCTGC CCCATCCAAA 
CACAGCTTCA CCTCTGACCG CTCCACATTC TCCACTCCTA AAAAGGAAAA CAGGAAGCCC 
AAAACAGGCA GGTCAAGCGA TGATCTCAGA GAGAAACACT CTGATAAGAA AAACGGCTTC 
TTTTCCTGGT ACAGATATCC AAGTATTGGG AAAGGACAAA AGAAAAGAGA GGCCCCAGAT 
TTACCCTACC ACTGTAATGG GCGGAGAGAG TCTCAAGGTT CTCTGTCTTC TGGAGCGAGT 
CAAGAGCTCA ACACTTCCTG TTCAGGAGGA AAGAATGAGC AGTGTGAGCT AAGAGGTTCG 
AGTTGTGCTT TGGATAAGGA GCGAGAGCGC TGCAGCAGGA CATGTACAGT AACACTGCCC 
GACGGCTCCA GCTGCTCTAT TCCTCTCCGA CAGGGAGCCT CCATCAGACA AGTGCTGCTA 
GAGCTCTGCC AAAAACAACA CATCAACCTG GCTGCTGTCG ACCTCTTTCT CACCGGTGGA 
GAGAAGCCTT TGGTCTTGGA TCAAGATAGT ATTACTCTCA GCTCCAGGGA ACTTCGTCTT 
GAGAAGCGCA CTTTGTTCAG ATTGGACCTG GTACCCATTA ACAGATCGGT TGGGTTGAAA 
GCGAAGCCCA CAAAACCTGT GACTGAGGTT CTACGGCCTG TGGTTGCCAA GTATGGACTC 
CAGCTTGGCG ATTTGGTGGC CAGAATAAGT GGCGAGACAG AGGTACTCGA TCTTGGAGTG 
CCCATATCAA ACCTTGATGG CCTGCGAGTT GTCTTGGAGA GGGCAGATCC AGCCAAAGCA 
GAAAAGTCTA AAACACTCAA CTCCAAAACA CCAGCTGCCA AGAAAGGGTT TCCAAGAGAG 
AGAGACGATA ACTCGACAGT GAAACCATTG AAAGCAGATG TTGAAGACAA GCATCCCCGC 
ACAGCTGCGT CTGAGAGGAA GAAAGGCAAA AAGAATCATA TAGATGAAAC TGAAGAGTTT 
TTCGAGTTGT TGAGTCGTGC TCAGAGCAGT CGTGTTGACG ACCAGAGAGG CCTGCTGAGA 
AAAGAAGATC TTGTCCTTCC CGACTTCCTT CGAGTAAACC CTGATCCCAA ACCTCAATCT 
CCTCCGTCCT GTTCAACCCC GACCTCTCAT AAACCCGGCC ACACCACTAC CACCTCCACA 
AACCCCCATC CCCCTAAACC CAGCCCCTGT AACGGACACC AAGCCTCAGC CCCGTTCAGC 
GCGCCACTCT CCCCCATCCT GCGTTCTGCT GGTGCCCGCG AGCAGGAGGA GGAGGAAGGC 
CTAGGAGATT TGACTCTGGT GGCGGAGGGT GACATCAGCA GCCCAAACTC CACCCTGCTC 
CCGCCTCCAC CGCCCGTATC ACCACCGCCT TATGATGGTA GTCTAGCAGA GGCCAACTTT 
ACTCCTCCAC CGGCTGTTCA CGGCACAGGT ACAGAAGAAG AAAACACGAG CACTTCAGCA 
GTAAACAGCT CAACAGCACA GCCGGACTTG CGTGTGCGGG ACAAGGGTGT CACTCTGGAG 
GACAGTTTTG AAGGATACGC AGCTGAACTT CGCCAGTGCC AGAACAAAAT GAGAAACGGC 
GTTTATCCCT CCACAGAAGC CCCCCGTCCC CTCAGCGGTG TGCTGGAAGT CAACGAGAGC 
GACACTGTGC AATACAAAGC AACGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301362.1
CDS	7..2319
Translation	MNPKSKTKRRSLLTISLMRRLSFRKRPPNLSAASDGERSGGGVQRHDCSSVSSLDSNGSLPSVTNHCRFTERRVALWAVSFERLLQDPVGVRYFSEFLKKEFSEENILFWQACECFSQVPEHDKKQLSQKAKEIYNRFLSSKATMPVNIDSQAQLADDVLTAPQPNMFKQPQLQIFNLMKMDSYARFLKSTLYQECMLAEVEGRPLPDPCPIPCSPAPSKHSFTSDRSTFSTPKKENRKPKTGRSSDDLREKHSDKKNGFFSWYRYPSIGKGQKKREAPDLPYHCNGRRESQGSLSSGASQELNTSCSGGKNEQCELRGSSCALDKERERCSRTCTVTLPDGSSCSIPLRQGASIRQVLLELCQKQHINLAAVDLFLTGGEKPLVLDQDSITLSSRELRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLQLGDLVARISGETEVLDLGVPISNLDGLRVVLERADPAKAEKSKTLNSKTPAAKKGFPRERDDNSTVKPLKADVEDKHPRTAASERKKGKKNHIDETEEFFELLSRAQSSRVDDQRGLLRKEDLVLPDFLRVNPDPKPQSPPSCSTPTSHKPGHTTTTSTNPHPPKPSPCNGHQASAPFSAPLSPILRSAGAREQEEEEGLGDLTLVAEGDISSPNSTLLPPPPPVSPPPYDGSLAEANFTPPPAVHGTGTEEENTSTSAVNSSTAQPDLRVRDKGVTLEDSFEGYAAELRQCQNKMRNGVYPSTEAPRPLSGVLEVNESDTVQYKATFV

Target ORF information:

RefSeq Version	XM_009303087.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio regulator of G protein signaling 12a (rgs12a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303087.3

ORF Insert Sequence:

ATGAATCCAA AATCAAAGAC TAAGAGAAGA AGCCTCTTGA CTATAAGTCT CATGAGGAGA 
CTTTCCTTCA GAAAGCGGCC CCCGAATCTG TCGGCTGCAT CAGATGGAGA GCGCAGTGGT 
GGTGGTGTCC AGCGTCACGA CTGCAGCAGC GTGAGCAGTT TGGACAGTAA CGGTAGTCTG 
CCCAGCGTCA CCAACCACTG TCGTTTCACA GAGCGCCGCG TTGCATTATG GGCAGTCAGC 
TTCGAGCGAT TGCTTCAGGA TCCTGTGGGT GTCCGCTATT TTTCTGAGTT TCTAAAGAAG 
GAGTTTAGCG AGGAAAACAT CTTATTCTGG CAGGCCTGTG AATGCTTCAG CCAAGTTCCA 
GAACATGACA AAAAGCAGCT GTCCCAGAAG GCCAAAGAGA TCTACAACCG TTTCCTCTCA 
AGTAAAGCCA CAATGCCAGT GAACATCGAC AGTCAAGCTC AACTTGCGGA CGATGTCCTG 
ACAGCTCCAC AGCCTAACAT GTTCAAACAG CCACAGCTAC AGATCTTCAA CCTGATGAAG 
ATGGACAGTT ATGCGCGCTT CTTGAAGTCC ACTCTCTATC AGGAGTGTAT GCTGGCAGAG 
GTGGAGGGTC GACCTCTGCC TGACCCCTGT CCCATCCCGT GCAGCCCTGC CCCATCCAAA 
CACAGCTTCA CCTCTGACCG CTCCACATTC TCCACTCCTA AAAAGGAAAA CAGGAAGCCC 
AAAACAGGCA GGTCAAGCGA TGATCTCAGA GAGAAACACT CTGATAAGAA AAACGGCTTC 
TTTTCCTGGT ACAGATATCC AAGTATTGGG AAAGGACAAA AGAAAAGAGA GGCCCCAGAT 
TTACCCTACC ACTGTAATGG GCGGAGAGAG TCTCAAGGTT CTCTGTCTTC TGGAGCGAGT 
CAAGAGCTCA ACACTTCCTG TTCAGGAGGA AAGAATGAGC AGTGTGAGCT AAGAGGTTCG 
AGTTGTGCTT TGGATAAGGA GCGAGAGCGC TGCAGCAGGA CATGTACAGT AACACTGCCC 
GACGGCTCCA GCTGCTCTAT TCCTCTCCGA CAGGGAGCCT CCATCAGACA AGTGCTGCTA 
GAGCTCTGCC AAAAACAACA CATCAACCTG GCTGCTGTCG ACCTCTTTCT CACCGGTGGA 
GAGAAGCCTT TGGTCTTGGA TCAAGATAGT ATTACTCTCA GCTCCAGGGA ACTTCGTCTT 
GAGAAGCGCA CTTTGTTCAG ATTGGACCTG GTACCCATTA ACAGATCGGT TGGGTTGAAA 
GCGAAGCCCA CAAAACCTGT GACTGAGGTT CTACGGCCTG TGGTTGCCAA GTATGGACTC 
CAGCTTGGCG ATTTGGTGGC CAGAATAAGT GGCGAGACAG AGGTACTCGA TCTTGGAGTG 
CCCATATCAA ACCTTGATGG CCTGCGAGTT GTCTTGGAGA GGGCAGATCC AGCCAAAGCA 
GAAAAGTCTA AAACACTCAA CTCCAAAACA CCAGCTGCCA AGAAAGGGTT TCCAAGAGAG 
AGAGACGATA ACTCGACAGT GAAACCATTG AAAGCAGATG TTGAAGACAA GCATCCCCGC 
ACAGCTGCGT CTGAGAGGAA GAAAGGCAAA AAGAATCATA TAGATGAAAC TGAAGAGTTT 
TTCGAGTTGT TGAGTCGTGC TCAGAGCAGT CGTGTTGACG ACCAGAGAGG CCTGCTGAGA 
AAAGAAGATC TTGTCCTTCC CGACTTCCTT CGAGTAAACC CTGATCCCAA ACCTCAATCT 
CCTCCGTCCT GTTCAACCCC GACCTCTCAT AAACCCGGCC ACACCACTAC CACCTCCACA 
AACCCCCATC CCCCTAAACC CAGCCCCTGT AACGGACACC AAGCCTCAGC CCCGTTCAGC 
GCGCCACTCT CCCCCATCCT GCGTTCTGCT GGTGCCCGCG AGCAGGAGGA GGAGGAAGGC 
CTAGGAGATT TGACTCTGGT GGCGGAGGGT GACATCAGCA GCCCAAACTC CACCCTGCTC 
CCGCCTCCAC CGCCCGTATC ACCACCGCCT TATGATGGTA GTCTAGCAGA GGCCAACTTT 
ACTCCTCCAC CGGCTGTTCA CGGCACAGGT ACAGAAGAAG AAAACACGAG CACTTCAGCA 
GTAAACAGCT CAACAGCACA GCCGGACTTG CGTGTGCGGG ACAAGGGTGT CACTCTGGAG 
GACAGTTTTG AAGGATACGC AGCTGAACTT CGCCAGTGCC AGAACAAAAT GAGAAACGGC 
GTTTATCCCT CCACAGAAGC CCCCCGTCCC CTCAGCGGTG TGCTGGAAGT CAACGAGAGC 
GACACTGTGC AATACAAAGC AACGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rgs12a ( XM_694005.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_699097.4 Danio rerio regulator of G-protein signaling 12a (rgs12a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa12809
Clone ID Related Accession (Same CDS sequence)	XM_694005.7 , XM_694005.8
Accession Version	XM_694005.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	regulator of G-protein signaling 12 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_694005.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTTT TGGACCGTCC CATCAGCCCG GCGGTCCGCA GGGTGGAAAT GGAGCGTGGC 
AGAGCTGGCT ATGGATTCAC CATTGCCGGT CAAGCACCAT GTGTTCTCAG AGGAATAATA 
AAAGGGAGTC CGGCGCACAG TGTAGGACTA CTACCTGGGG ATCGTGTCTT GCGTGTAAAT 
GACACAGATG TGTCTGAAGC ATCACATGAT GTTGTGGTGA AGTTGATCGG TACTTCATCA 
AAGTCACTGT GTTTGCTGGT CACATCAGGT GATCGACCAC CCATGGCCTG CTGCTCCAGT 
GACGAGGAAC TGGGTTGTCA GAATAAGAGT AAACGTCCGT GGCTCAATCC TGGAATAAAG 
GACCCTTTCC CAGGTAAACT TGATGGCAGG GATTTGAAGG AACCAATTCT TCAGTCGGTT 
GGAAGTTTTG ATACCGTCTT TGAGATCTCA GATCAGGGCA CCATGACCCC ACATCAGGAG 
AAGCAGAAAA AACAAAGACC TTTGTCTGAA CCAGATGTGT CTTACTGGTT GCAGCAGCAG 
CAGAGATCCA AAGGGAATGT CGATATTCTT TCACAGGATG AAACATCTCG AGTAGTGAGT 
GAGGATTCTG TGTTTTCAGA CCTTCAGAGT CAGAATGACC TTGCATCTGA CTCCAGCATC 
CTGAACGTTG CAATGATTGT CGGATACATT AATTCCATGG AACTGGAGCT AGTAATGTCA 
CAAACTGAGG AGGATGCATT GCAGGTCATC CGGCAATGCA TTAGTAAGAT TGGTATTGAG 
CAGAAGACTC ATTCGCTGGT CTTAATGAAG GTTAAATGCA ACTGTGTTCA ACTTTGCGAT 
GACCGAGATG TTGTTCTTGC ATCTTACCCA GCAGAAAACC TGGCTCTTTG CGTAATTTGC 
ACAGAAGACA GCAGGTTCTT TGGCCTTGTC TCGATTGATT CAACCAAAAA TATAGACACC 
GGGAGGCAGA CAAGTACCTT GAAGACATTA TGTCATGTTT TTTTCATAGA CCCAGAGCTT 
TATGAACACA AGGAGCACCA AAGCATCATC GGACACTTTG GGATTCCCTG CACCCCAGAT 
CCAGACACTC AAAGCTGTTT GGAGTTTCCT CAAACCCCCC ACTCCATTTC ACACTTCATA 
TCCGTCCTCT ATGCAGACAT GGGAGATTAC ATTGAAAAAC TAAGACTTAA ACTTGACAGT 
GAGAATCCTG ACGAAGGATC CCAAAATGCA CGGAACAGCG GCAGTGGCAG CGGCAGTGAT 
AGCGGGATTG GAAATGCTTC ACCAGAGGAA AGAGACAGTC TCATTGGTCA GTGGAGCTTC 
ACATCTCCAA ACATTTGCAA TCCACCACCA CCATATTATT CCCAGCACAG GAATAGTACA 
TTCCTCAAAC CAGAGTGCAG ATGCCTTCTT TCTGAGCCAC TAACAGGATC CGCTCAACCT 
GCGACTCCAT CAGGTGTCCC AGCCGGGGCG GACGGTTCAG CGTTTACTCC TGTTCCTCTC 
AGTAAGCAAT CTAACTTGCC TCCAAAACAA AGAGCCGGTC AACGAAGACC TTCCATTGGC 
TTAAAGGCTC GCTGGCAGAA ATCTCGCCCC AACAGCATTG AGTGCTTAGT GGAAGGGGAC 
AACGATGATC CCAAAAGTAA ATGCAGTACC CTTCCACCTG CAATGGAGCA AATCCCTACT 
CTGAGATACA AACCTTTAGA ACATACTCAG AGAATAAACT TCACCCCGCA GCTTGAGCTG 
ACATCCAGGT TCTTCAGCAG TGCAGCTAAG CAGAAGGATG GAGAAAACAA GGGATCTTTA 
TTTTGGGCTC TCACAAGGGG ACGCTCTTCT GTTCGAAGAC CGTCAGGTCC CGCTAAACGA 
CTCAGTCTGG CTCATTCACT TAATGATCTT GAGTCGGCTG CATCAGATGG AGAGCGCAGT 
GGTGGTGGTG TCCAGCGTCA CGACTGCAGC AGCGTGAGCA GTTTGGACAG TAACGGTAGT 
CTGCCCAGCG TCACCAACCA CTGTCGTTTC ACAGAGCGCC GCGTTGCATT ATGGGCAGTC 
AGCTTCGAGC GATTGCTTCA GGATCCTGTG GGTGTCCGCT ATTTTTCTGA GTTTCTAAAG 
AAGGAGTTTA GCGAGGAAAA CATCTTATTC TGGCAGGCCT GTGAATGCTT CAGCCAAGTT 
CCAGAACATG ACAAAAAGCA GCTGTCCCAG AAGGCCAAAG AGATCTACAA CCGTTTCCTC 
TCAAGTAAAG CCACAATGCC AGTGAACATC GACAGTCAAG CTCAACTTGC GGACGATGTC 
CTGACAGCTC CACAGCCTAA CATGTTCAAA CAGCCACAGC TACAGATCTT CAACCTGATG 
AAGATGGACA GTTATGCGCG CTTCTTGAAG TCCACTCTCT ATCAGGAGTG TATGCTGGCA 
GAGGTGGAGG GTCGACCTCT GCCTGACCCC TGTCCCATCC CGTGCAGCCC TGCCCCATCC 
AAACACAGCT TCACCTCTGA CCGCTCCACA TTCTCCACTC CTAAAAAGGA AAACAGGAAG 
CCCAAAACAG GCAGGTCAAG CGATGATCTC AGAGAGAAAC ACTCTGATAA GAAAAACGGC 
TTCTTTTCCT GGTACAGATA TCCAAGTATT GGGAAAGGAC AAAAGAAAAG AGAGGCCCCA 
GATTTACCCT ACCACTGTAA TGGGCGGAGA GAGTCTCAAG GTTCTCTGTC TTCTGGAGCG 
AGTCAAGAGC TCAACACTTC CTGTTCAGGA GGAAAGAATG AGCAGTGTGA GCTAAGAGGT 
TCGAGTTGTG CTTTGGATAA GGAGCGAGAG CGCTGCAGCA GGACATGTAC AGTAACACTG 
CCCGACGGCT CCAGCTGCTC TATTCCTCTC CGACAGGGAG CCTCCATCAG ACAAGTGCTG 
CTAGAGCTCT GCCAAAAACA ACACATCAAC CTGGCTGCTG TCGACCTCTT TCTCACCGGT 
GGAGAGAAGC CTTTGGTCTT GGATCAAGAT AGTATTACTC TCAGCTCCAG GGAACTTCGT 
CTTGAGAAGC GCACTTTGTT CAGATTGGAC CTGGTACCCA TTAACAGATC GGTTGGGTTG 
AAAGCGAAGC CCACAAAACC TGTGACTGAG GTTCTACGGC CTGTGGTTGC CAAGTATGGA 
CTCCAGCTTG GCGATTTGGT GGCCAGAATA AGTGGCGAGA CAGAGGTACT CGATCTTGGA 
GTGCCCATAT CAAACCTTGA TGGCCTGCGA GTTGTCTTGG AGAGGGCAGA TCCAGCCAAA 
GCAGAAAAGT CTAAAACACT CAACTCCAAA ACACCAGCTG CCAAGAAAGG GTTTCCAAGA 
GAGAGAGACG ATAACTCGAC AGTGAAACCA TTGAAAGCAG ATGTTGAAGA CAAGCATCCC 
CGCACAGCTG CGTCTGAGAG GAAGAAAGGC AAAAAGAATC ATATAGATGA AACTGAAGAG 
TTTTTCGAGT TGTTGAGTCG TGCTCAGAGC AGTCGTGTTG ACGACCAGAG AGGCCTGCTG 
AGAAAAGAAG ATCTTGTCCT TCCCGACTTC CTTCGAGTAA ACCCTGATCC CAAACCTCAA 
TCTCCTCCGT CCTGTTCAAC CCCGACCTCT CATAAACCCG GCCACACCAC TACCACCTCC 
ACAAACCCCC ATCCCCCTAA ACCCAGCCCC TGTAACGGAC ACCAAGCCTC AGCCCCGTTC 
AGCGCGCCAC TCTCCCCCAT CCTGCGTTCT GCTGGTGCCC GCGAGCAGGA GGAGGAGGAA 
GGCCTAGGAG ATTTGACTCT GGTGGCGGAG GGTGACATCA GCAGCCCAAA CTCCACCCTG 
CTCCCGCCTC CACCGCCCGT ATCACCACCG CCTTATGATG GTAGTCTAGC AGAGGCCAAC 
TTTACTCCTC CACCGGCTGT TCACGGCACA GGTACAGAAG AAGAAAACAC GAGCACTTCA 
GCAGTAAACA GCTCAACAGC ACAGCCGGAC TTGCGTGTGC GGGACAAGGG TGTCACTCTG 
GAGGACAGTT TTGAAGGATA CGCAGCTGAA CTTCGCCAGT GCCAGAACAA AATGAGAAAC 
GGCGTTTATC CCTCCACAGA AGCCCCCCGT CCCCTCAGCG GTGTGCTGGA AGTCAACGAG 
AGCGACACTG TGCAATACAA AGCAACGTTT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_699097.4
CDS	241..4356
Translation	MRLLDRPISPAVRRVEMERGRAGYGFTIAGQAPCVLRGIIKGSPAHSVGLLPGDRVLRVNDTDVSEASHDVVVKLIGTSSKSLCLLVTSGDRPPMACCSSDEELGCQNKSKRPWLNPGIKDPFPGKLDGRDLKEPILQSVGSFDTVFEISDQGTMTPHQEKQKKQRPLSEPDVSYWLQQQQRSKGNVDILSQDETSRVVSEDSVFSDLQSQNDLASDSSILNVAMIVGYINSMELELVMSQTEEDALQVIRQCISKIGIEQKTHSLVLMKVKCNCVQLCDDRDVVLASYPAENLALCVICTEDSRFFGLVSIDSTKNIDTGRQTSTLKTLCHVFFIDPELYEHKEHQSIIGHFGIPCTPDPDTQSCLEFPQTPHSISHFISVLYADMGDYIEKLRLKLDSENPDEGSQNARNSGSGSGSDSGIGNASPEERDSLIGQWSFTSPNICNPPPPYYSQHRNSTFLKPECRCLLSEPLTGSAQPATPSGVPAGADGSAFTPVPLSKQSNLPPKQRAGQRRPSIGLKARWQKSRPNSIECLVEGDNDDPKSKCSTLPPAMEQIPTLRYKPLEHTQRINFTPQLELTSRFFSSAAKQKDGENKGSLFWALTRGRSSVRRPSGPAKRLSLAHSLNDLESAASDGERSGGGVQRHDCSSVSSLDSNGSLPSVTNHCRFTERRVALWAVSFERLLQDPVGVRYFSEFLKKEFSEENILFWQACECFSQVPEHDKKQLSQKAKEIYNRFLSSKATMPVNIDSQAQLADDVLTAPQPNMFKQPQLQIFNLMKMDSYARFLKSTLYQECMLAEVEGRPLPDPCPIPCSPAPSKHSFTSDRSTFSTPKKENRKPKTGRSSDDLREKHSDKKNGFFSWYRYPSIGKGQKKREAPDLPYHCNGRRESQGSLSSGASQELNTSCSGGKNEQCELRGSSCALDKERERCSRTCTVTLPDGSSCSIPLRQGASIRQVLLELCQKQHINLAAVDLFLTGGEKPLVLDQDSITLSSRELRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLQLGDLVARISGETEVLDLGVPISNLDGLRVVLERADPAKAEKSKTLNSKTPAAKKGFPRERDDNSTVKPLKADVEDKHPRTAASERKKGKKNHIDETEEFFELLSRAQSSRVDDQRGLLRKEDLVLPDFLRVNPDPKPQSPPSCSTPTSHKPGHTTTTSTNPHPPKPSPCNGHQASAPFSAPLSPILRSAGAREQEEEEGLGDLTLVAEGDISSPNSTLLPPPPPVSPPPYDGSLAEANFTPPPAVHGTGTEEENTSTSAVNSSTAQPDLRVRDKGVTLEDSFEGYAAELRQCQNKMRNGVYPSTEAPRPLSGVLEVNESDTVQYKATFV

Target ORF information:

RefSeq Version	XM_694005.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio regulator of G-protein signaling 12a (rgs12a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_694005.7

ORF Insert Sequence:

ATGAGGCTTT TGGACCGTCC CATCAGCCCG GCGGTCCGCA GGGTGGAAAT GGAGCGTGGC 
AGAGCTGGCT ATGGATTCAC CATTGCCGGT CAAGCACCAT GTGTTCTCAG AGGAATAATA 
AAAGGGAGTC CGGCGCACAG TGTAGGACTA CTACCTGGGG ATCGTGTCTT GCGTGTAAAT 
GACACAGATG TGTCTGAAGC ATCACATGAT GTTGTGGTGA AGTTGATCGG TACTTCATCA 
AAGTCACTGT GTTTGCTGGT CACATCAGGT GATCGACCAC CCATGGCCTG CTGCTCCAGT 
GACGAGGAAC TGGGTTGTCA GAATAAGAGT AAACGTCCGT GGCTCAATCC TGGAATAAAG 
GACCCTTTCC CAGGTAAACT TGATGGCAGG GATTTGAAGG AACCAATTCT TCAGTCGGTT 
GGAAGTTTTG ATACCGTCTT TGAGATCTCA GATCAGGGCA CCATGACCCC ACATCAGGAG 
AAGCAGAAAA AACAAAGACC TTTGTCTGAA CCAGATGTGT CTTACTGGTT GCAGCAGCAG 
CAGAGATCCA AAGGGAATGT CGATATTCTT TCACAGGATG AAACATCTCG AGTAGTGAGT 
GAGGATTCTG TGTTTTCAGA CCTTCAGAGT CAGAATGACC TTGCATCTGA CTCCAGCATC 
CTGAACGTTG CAATGATTGT CGGATACATT AATTCCATGG AACTGGAGCT AGTAATGTCA 
CAAACTGAGG AGGATGCATT GCAGGTCATC CGGCAATGCA TTAGTAAGAT TGGTATTGAG 
CAGAAGACTC ATTCGCTGGT CTTAATGAAG GTTAAATGCA ACTGTGTTCA ACTTTGCGAT 
GACCGAGATG TTGTTCTTGC ATCTTACCCA GCAGAAAACC TGGCTCTTTG CGTAATTTGC 
ACAGAAGACA GCAGGTTCTT TGGCCTTGTC TCGATTGATT CAACCAAAAA TATAGACACC 
GGGAGGCAGA CAAGTACCTT GAAGACATTA TGTCATGTTT TTTTCATAGA CCCAGAGCTT 
TATGAACACA AGGAGCACCA AAGCATCATC GGACACTTTG GGATTCCCTG CACCCCAGAT 
CCAGACACTC AAAGCTGTTT GGAGTTTCCT CAAACCCCCC ACTCCATTTC ACACTTCATA 
TCCGTCCTCT ATGCAGACAT GGGAGATTAC ATTGAAAAAC TAAGACTTAA ACTTGACAGT 
GAGAATCCTG ACGAAGGATC CCAAAATGCA CGGAACAGCG GCAGTGGCAG CGGCAGTGAT 
AGCGGGATTG GAAATGCTTC ACCAGAGGAA AGAGACAGTC TCATTGGTCA GTGGAGCTTC 
ACATCTCCAA ACATTTGCAA TCCACCACCA CCATATTATT CCCAGCACAG GAATAGTACA 
TTCCTCAAAC CAGAGTGCAG ATGCCTTCTT TCTGAGCCAC TAACAGGATC CGCTCAACCT 
GCGACTCCAT CAGGTGTCCC AGCCGGGGCG GACGGTTCAG CGTTTACTCC TGTTCCTCTC 
AGTAAGCAAT CTAACTTGCC TCCAAAACAA AGAGCCGGTC AACGAAGACC TTCCATTGGC 
TTAAAGGCTC GCTGGCAGAA ATCTCGCCCC AACAGCATTG AGTGCTTAGT GGAAGGGGAC 
AACGATGATC CCAAAAGTAA ATGCAGTACC CTTCCACCTG CAATGGAGCA AATCCCTACT 
CTGAGATACA AACCTTTAGA ACATACTCAG AGAATAAACT TCACCCCGCA GCTTGAGCTG 
ACATCCAGGT TCTTCAGCAG TGCAGCTAAG CAGAAGGATG GAGAAAACAA GGGATCTTTA 
TTTTGGGCTC TCACAAGGGG ACGCTCTTCT GTTCGAAGAC CGTCAGGTCC CGCTAAACGA 
CTCAGTCTGG CTCATTCACT TAATGATCTT GAGTCGGCTG CATCAGATGG AGAGCGCAGT 
GGTGGTGGTG TCCAGCGTCA CGACTGCAGC AGCGTGAGCA GTTTGGACAG TAACGGTAGT 
CTGCCCAGCG TCACCAACCA CTGTCGTTTC ACAGAGCGCC GCGTTGCATT ATGGGCAGTC 
AGCTTCGAGC GATTGCTTCA GGATCCTGTG GGTGTCCGCT ATTTTTCTGA GTTTCTAAAG 
AAGGAGTTTA GCGAGGAAAA CATCTTATTC TGGCAGGCCT GTGAATGCTT CAGCCAAGTT 
CCAGAACATG ACAAAAAGCA GCTGTCCCAG AAGGCCAAAG AGATCTACAA CCGTTTCCTC 
TCAAGTAAAG CCACAATGCC AGTGAACATC GACAGTCAAG CTCAACTTGC GGACGATGTC 
CTGACAGCTC CACAGCCTAA CATGTTCAAA CAGCCACAGC TACAGATCTT CAACCTGATG 
AAGATGGACA GTTATGCGCG CTTCTTGAAG TCCACTCTCT ATCAGGAGTG TATGCTGGCA 
GAGGTGGAGG GTCGACCTCT GCCTGACCCC TGTCCCATCC CGTGCAGCCC TGCCCCATCC 
AAACACAGCT TCACCTCTGA CCGCTCCACA TTCTCCACTC CTAAAAAGGA AAACAGGAAG 
CCCAAAACAG GCAGGTCAAG CGATGATCTC AGAGAGAAAC ACTCTGATAA GAAAAACGGC 
TTCTTTTCCT GGTACAGATA TCCAAGTATT GGGAAAGGAC AAAAGAAAAG AGAGGCCCCA 
GATTTACCCT ACCACTGTAA TGGGCGGAGA GAGTCTCAAG GTTCTCTGTC TTCTGGAGCG 
AGTCAAGAGC TCAACACTTC CTGTTCAGGA GGAAAGAATG AGCAGTGTGA GCTAAGAGGT 
TCGAGTTGTG CTTTGGATAA GGAGCGAGAG CGCTGCAGCA GGACATGTAC AGTAACACTG 
CCCGACGGCT CCAGCTGCTC TATTCCTCTC CGACAGGGAG CCTCCATCAG ACAAGTGCTG 
CTAGAGCTCT GCCAAAAACA ACACATCAAC CTGGCTGCTG TCGACCTCTT TCTCACCGGT 
GGAGAGAAGC CTTTGGTCTT GGATCAAGAT AGTATTACTC TCAGCTCCAG GGAACTTCGT 
CTTGAGAAGC GCACTTTGTT CAGATTGGAC CTGGTACCCA TTAACAGATC GGTTGGGTTG 
AAAGCGAAGC CCACAAAACC TGTGACTGAG GTTCTACGGC CTGTGGTTGC CAAGTATGGA 
CTCCAGCTTG GCGATTTGGT GGCCAGAATA AGTGGCGAGA CAGAGGTACT CGATCTTGGA 
GTGCCCATAT CAAACCTTGA TGGCCTGCGA GTTGTCTTGG AGAGGGCAGA TCCAGCCAAA 
GCAGAAAAGT CTAAAACACT CAACTCCAAA ACACCAGCTG CCAAGAAAGG GTTTCCAAGA 
GAGAGAGACG ATAACTCGAC AGTGAAACCA TTGAAAGCAG ATGTTGAAGA CAAGCATCCC 
CGCACAGCTG CGTCTGAGAG GAAGAAAGGC AAAAAGAATC ATATAGATGA AACTGAAGAG 
TTTTTCGAGT TGTTGAGTCG TGCTCAGAGC AGTCGTGTTG ACGACCAGAG AGGCCTGCTG 
AGAAAAGAAG ATCTTGTCCT TCCCGACTTC CTTCGAGTAA ACCCTGATCC CAAACCTCAA 
TCTCCTCCGT CCTGTTCAAC CCCGACCTCT CATAAACCCG GCCACACCAC TACCACCTCC 
ACAAACCCCC ATCCCCCTAA ACCCAGCCCC TGTAACGGAC ACCAAGCCTC AGCCCCGTTC 
AGCGCGCCAC TCTCCCCCAT CCTGCGTTCT GCTGGTGCCC GCGAGCAGGA GGAGGAGGAA 
GGCCTAGGAG ATTTGACTCT GGTGGCGGAG GGTGACATCA GCAGCCCAAA CTCCACCCTG 
CTCCCGCCTC CACCGCCCGT ATCACCACCG CCTTATGATG GTAGTCTAGC AGAGGCCAAC 
TTTACTCCTC CACCGGCTGT TCACGGCACA GGTACAGAAG AAGAAAACAC GAGCACTTCA 
GCAGTAAACA GCTCAACAGC ACAGCCGGAC TTGCGTGTGC GGGACAAGGG TGTCACTCTG 
GAGGACAGTT TTGAAGGATA CGCAGCTGAA CTTCGCCAGT GCCAGAACAA AATGAGAAAC 
GGCGTTTATC CCTCCACAGA AGCCCCCCGT CCCCTCAGCG GTGTGCTGGA AGTCAACGAG 
AGCGACACTG TGCAATACAA AGCAACGTTT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rgs12a ( XM_694005.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_699097.4 Danio rerio regulator of G protein signaling 12a (rgs12a), transcript variant X1, mRNA.

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CloneID	ODa12809
Clone ID Related Accession (Same CDS sequence)	XM_694005.7 , XM_694005.8
Accession Version	XM_694005.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	regulator of G-protein signaling 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_694005.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTTT TGGACCGTCC CATCAGCCCG GCGGTCCGCA GGGTGGAAAT GGAGCGTGGC 
AGAGCTGGCT ATGGATTCAC CATTGCCGGT CAAGCACCAT GTGTTCTCAG AGGAATAATA 
AAAGGGAGTC CGGCGCACAG TGTAGGACTA CTACCTGGGG ATCGTGTCTT GCGTGTAAAT 
GACACAGATG TGTCTGAAGC ATCACATGAT GTTGTGGTGA AGTTGATCGG TACTTCATCA 
AAGTCACTGT GTTTGCTGGT CACATCAGGT GATCGACCAC CCATGGCCTG CTGCTCCAGT 
GACGAGGAAC TGGGTTGTCA GAATAAGAGT AAACGTCCGT GGCTCAATCC TGGAATAAAG 
GACCCTTTCC CAGGTAAACT TGATGGCAGG GATTTGAAGG AACCAATTCT TCAGTCGGTT 
GGAAGTTTTG ATACCGTCTT TGAGATCTCA GATCAGGGCA CCATGACCCC ACATCAGGAG 
AAGCAGAAAA AACAAAGACC TTTGTCTGAA CCAGATGTGT CTTACTGGTT GCAGCAGCAG 
CAGAGATCCA AAGGGAATGT CGATATTCTT TCACAGGATG AAACATCTCG AGTAGTGAGT 
GAGGATTCTG TGTTTTCAGA CCTTCAGAGT CAGAATGACC TTGCATCTGA CTCCAGCATC 
CTGAACGTTG CAATGATTGT CGGATACATT AATTCCATGG AACTGGAGCT AGTAATGTCA 
CAAACTGAGG AGGATGCATT GCAGGTCATC CGGCAATGCA TTAGTAAGAT TGGTATTGAG 
CAGAAGACTC ATTCGCTGGT CTTAATGAAG GTTAAATGCA ACTGTGTTCA ACTTTGCGAT 
GACCGAGATG TTGTTCTTGC ATCTTACCCA GCAGAAAACC TGGCTCTTTG CGTAATTTGC 
ACAGAAGACA GCAGGTTCTT TGGCCTTGTC TCGATTGATT CAACCAAAAA TATAGACACC 
GGGAGGCAGA CAAGTACCTT GAAGACATTA TGTCATGTTT TTTTCATAGA CCCAGAGCTT 
TATGAACACA AGGAGCACCA AAGCATCATC GGACACTTTG GGATTCCCTG CACCCCAGAT 
CCAGACACTC AAAGCTGTTT GGAGTTTCCT CAAACCCCCC ACTCCATTTC ACACTTCATA 
TCCGTCCTCT ATGCAGACAT GGGAGATTAC ATTGAAAAAC TAAGACTTAA ACTTGACAGT 
GAGAATCCTG ACGAAGGATC CCAAAATGCA CGGAACAGCG GCAGTGGCAG CGGCAGTGAT 
AGCGGGATTG GAAATGCTTC ACCAGAGGAA AGAGACAGTC TCATTGGTCA GTGGAGCTTC 
ACATCTCCAA ACATTTGCAA TCCACCACCA CCATATTATT CCCAGCACAG GAATAGTACA 
TTCCTCAAAC CAGAGTGCAG ATGCCTTCTT TCTGAGCCAC TAACAGGATC CGCTCAACCT 
GCGACTCCAT CAGGTGTCCC AGCCGGGGCG GACGGTTCAG CGTTTACTCC TGTTCCTCTC 
AGTAAGCAAT CTAACTTGCC TCCAAAACAA AGAGCCGGTC AACGAAGACC TTCCATTGGC 
TTAAAGGCTC GCTGGCAGAA ATCTCGCCCC AACAGCATTG AGTGCTTAGT GGAAGGGGAC 
AACGATGATC CCAAAAGTAA ATGCAGTACC CTTCCACCTG CAATGGAGCA AATCCCTACT 
CTGAGATACA AACCTTTAGA ACATACTCAG AGAATAAACT TCACCCCGCA GCTTGAGCTG 
ACATCCAGGT TCTTCAGCAG TGCAGCTAAG CAGAAGGATG GAGAAAACAA GGGATCTTTA 
TTTTGGGCTC TCACAAGGGG ACGCTCTTCT GTTCGAAGAC CGTCAGGTCC CGCTAAACGA 
CTCAGTCTGG CTCATTCACT TAATGATCTT GAGTCGGCTG CATCAGATGG AGAGCGCAGT 
GGTGGTGGTG TCCAGCGTCA CGACTGCAGC AGCGTGAGCA GTTTGGACAG TAACGGTAGT 
CTGCCCAGCG TCACCAACCA CTGTCGTTTC ACAGAGCGCC GCGTTGCATT ATGGGCAGTC 
AGCTTCGAGC GATTGCTTCA GGATCCTGTG GGTGTCCGCT ATTTTTCTGA GTTTCTAAAG 
AAGGAGTTTA GCGAGGAAAA CATCTTATTC TGGCAGGCCT GTGAATGCTT CAGCCAAGTT 
CCAGAACATG ACAAAAAGCA GCTGTCCCAG AAGGCCAAAG AGATCTACAA CCGTTTCCTC 
TCAAGTAAAG CCACAATGCC AGTGAACATC GACAGTCAAG CTCAACTTGC GGACGATGTC 
CTGACAGCTC CACAGCCTAA CATGTTCAAA CAGCCACAGC TACAGATCTT CAACCTGATG 
AAGATGGACA GTTATGCGCG CTTCTTGAAG TCCACTCTCT ATCAGGAGTG TATGCTGGCA 
GAGGTGGAGG GTCGACCTCT GCCTGACCCC TGTCCCATCC CGTGCAGCCC TGCCCCATCC 
AAACACAGCT TCACCTCTGA CCGCTCCACA TTCTCCACTC CTAAAAAGGA AAACAGGAAG 
CCCAAAACAG GCAGGTCAAG CGATGATCTC AGAGAGAAAC ACTCTGATAA GAAAAACGGC 
TTCTTTTCCT GGTACAGATA TCCAAGTATT GGGAAAGGAC AAAAGAAAAG AGAGGCCCCA 
GATTTACCCT ACCACTGTAA TGGGCGGAGA GAGTCTCAAG GTTCTCTGTC TTCTGGAGCG 
AGTCAAGAGC TCAACACTTC CTGTTCAGGA GGAAAGAATG AGCAGTGTGA GCTAAGAGGT 
TCGAGTTGTG CTTTGGATAA GGAGCGAGAG CGCTGCAGCA GGACATGTAC AGTAACACTG 
CCCGACGGCT CCAGCTGCTC TATTCCTCTC CGACAGGGAG CCTCCATCAG ACAAGTGCTG 
CTAGAGCTCT GCCAAAAACA ACACATCAAC CTGGCTGCTG TCGACCTCTT TCTCACCGGT 
GGAGAGAAGC CTTTGGTCTT GGATCAAGAT AGTATTACTC TCAGCTCCAG GGAACTTCGT 
CTTGAGAAGC GCACTTTGTT CAGATTGGAC CTGGTACCCA TTAACAGATC GGTTGGGTTG 
AAAGCGAAGC CCACAAAACC TGTGACTGAG GTTCTACGGC CTGTGGTTGC CAAGTATGGA 
CTCCAGCTTG GCGATTTGGT GGCCAGAATA AGTGGCGAGA CAGAGGTACT CGATCTTGGA 
GTGCCCATAT CAAACCTTGA TGGCCTGCGA GTTGTCTTGG AGAGGGCAGA TCCAGCCAAA 
GCAGAAAAGT CTAAAACACT CAACTCCAAA ACACCAGCTG CCAAGAAAGG GTTTCCAAGA 
GAGAGAGACG ATAACTCGAC AGTGAAACCA TTGAAAGCAG ATGTTGAAGA CAAGCATCCC 
CGCACAGCTG CGTCTGAGAG GAAGAAAGGC AAAAAGAATC ATATAGATGA AACTGAAGAG 
TTTTTCGAGT TGTTGAGTCG TGCTCAGAGC AGTCGTGTTG ACGACCAGAG AGGCCTGCTG 
AGAAAAGAAG ATCTTGTCCT TCCCGACTTC CTTCGAGTAA ACCCTGATCC CAAACCTCAA 
TCTCCTCCGT CCTGTTCAAC CCCGACCTCT CATAAACCCG GCCACACCAC TACCACCTCC 
ACAAACCCCC ATCCCCCTAA ACCCAGCCCC TGTAACGGAC ACCAAGCCTC AGCCCCGTTC 
AGCGCGCCAC TCTCCCCCAT CCTGCGTTCT GCTGGTGCCC GCGAGCAGGA GGAGGAGGAA 
GGCCTAGGAG ATTTGACTCT GGTGGCGGAG GGTGACATCA GCAGCCCAAA CTCCACCCTG 
CTCCCGCCTC CACCGCCCGT ATCACCACCG CCTTATGATG GTAGTCTAGC AGAGGCCAAC 
TTTACTCCTC CACCGGCTGT TCACGGCACA GGTACAGAAG AAGAAAACAC GAGCACTTCA 
GCAGTAAACA GCTCAACAGC ACAGCCGGAC TTGCGTGTGC GGGACAAGGG TGTCACTCTG 
GAGGACAGTT TTGAAGGATA CGCAGCTGAA CTTCGCCAGT GCCAGAACAA AATGAGAAAC 
GGCGTTTATC CCTCCACAGA AGCCCCCCGT CCCCTCAGCG GTGTGCTGGA AGTCAACGAG 
AGCGACACTG TGCAATACAA AGCAACGTTT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_699097.4
CDS	264..4379
Translation	MRLLDRPISPAVRRVEMERGRAGYGFTIAGQAPCVLRGIIKGSPAHSVGLLPGDRVLRVNDTDVSEASHDVVVKLIGTSSKSLCLLVTSGDRPPMACCSSDEELGCQNKSKRPWLNPGIKDPFPGKLDGRDLKEPILQSVGSFDTVFEISDQGTMTPHQEKQKKQRPLSEPDVSYWLQQQQRSKGNVDILSQDETSRVVSEDSVFSDLQSQNDLASDSSILNVAMIVGYINSMELELVMSQTEEDALQVIRQCISKIGIEQKTHSLVLMKVKCNCVQLCDDRDVVLASYPAENLALCVICTEDSRFFGLVSIDSTKNIDTGRQTSTLKTLCHVFFIDPELYEHKEHQSIIGHFGIPCTPDPDTQSCLEFPQTPHSISHFISVLYADMGDYIEKLRLKLDSENPDEGSQNARNSGSGSGSDSGIGNASPEERDSLIGQWSFTSPNICNPPPPYYSQHRNSTFLKPECRCLLSEPLTGSAQPATPSGVPAGADGSAFTPVPLSKQSNLPPKQRAGQRRPSIGLKARWQKSRPNSIECLVEGDNDDPKSKCSTLPPAMEQIPTLRYKPLEHTQRINFTPQLELTSRFFSSAAKQKDGENKGSLFWALTRGRSSVRRPSGPAKRLSLAHSLNDLESAASDGERSGGGVQRHDCSSVSSLDSNGSLPSVTNHCRFTERRVALWAVSFERLLQDPVGVRYFSEFLKKEFSEENILFWQACECFSQVPEHDKKQLSQKAKEIYNRFLSSKATMPVNIDSQAQLADDVLTAPQPNMFKQPQLQIFNLMKMDSYARFLKSTLYQECMLAEVEGRPLPDPCPIPCSPAPSKHSFTSDRSTFSTPKKENRKPKTGRSSDDLREKHSDKKNGFFSWYRYPSIGKGQKKREAPDLPYHCNGRRESQGSLSSGASQELNTSCSGGKNEQCELRGSSCALDKERERCSRTCTVTLPDGSSCSIPLRQGASIRQVLLELCQKQHINLAAVDLFLTGGEKPLVLDQDSITLSSRELRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLQLGDLVARISGETEVLDLGVPISNLDGLRVVLERADPAKAEKSKTLNSKTPAAKKGFPRERDDNSTVKPLKADVEDKHPRTAASERKKGKKNHIDETEEFFELLSRAQSSRVDDQRGLLRKEDLVLPDFLRVNPDPKPQSPPSCSTPTSHKPGHTTTTSTNPHPPKPSPCNGHQASAPFSAPLSPILRSAGAREQEEEEGLGDLTLVAEGDISSPNSTLLPPPPPVSPPPYDGSLAEANFTPPPAVHGTGTEEENTSTSAVNSSTAQPDLRVRDKGVTLEDSFEGYAAELRQCQNKMRNGVYPSTEAPRPLSGVLEVNESDTVQYKATFV

Target ORF information:

RefSeq Version	XM_694005.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio regulator of G protein signaling 12a (rgs12a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_694005.8

ORF Insert Sequence:

ATGAGGCTTT TGGACCGTCC CATCAGCCCG GCGGTCCGCA GGGTGGAAAT GGAGCGTGGC 
AGAGCTGGCT ATGGATTCAC CATTGCCGGT CAAGCACCAT GTGTTCTCAG AGGAATAATA 
AAAGGGAGTC CGGCGCACAG TGTAGGACTA CTACCTGGGG ATCGTGTCTT GCGTGTAAAT 
GACACAGATG TGTCTGAAGC ATCACATGAT GTTGTGGTGA AGTTGATCGG TACTTCATCA 
AAGTCACTGT GTTTGCTGGT CACATCAGGT GATCGACCAC CCATGGCCTG CTGCTCCAGT 
GACGAGGAAC TGGGTTGTCA GAATAAGAGT AAACGTCCGT GGCTCAATCC TGGAATAAAG 
GACCCTTTCC CAGGTAAACT TGATGGCAGG GATTTGAAGG AACCAATTCT TCAGTCGGTT 
GGAAGTTTTG ATACCGTCTT TGAGATCTCA GATCAGGGCA CCATGACCCC ACATCAGGAG 
AAGCAGAAAA AACAAAGACC TTTGTCTGAA CCAGATGTGT CTTACTGGTT GCAGCAGCAG 
CAGAGATCCA AAGGGAATGT CGATATTCTT TCACAGGATG AAACATCTCG AGTAGTGAGT 
GAGGATTCTG TGTTTTCAGA CCTTCAGAGT CAGAATGACC TTGCATCTGA CTCCAGCATC 
CTGAACGTTG CAATGATTGT CGGATACATT AATTCCATGG AACTGGAGCT AGTAATGTCA 
CAAACTGAGG AGGATGCATT GCAGGTCATC CGGCAATGCA TTAGTAAGAT TGGTATTGAG 
CAGAAGACTC ATTCGCTGGT CTTAATGAAG GTTAAATGCA ACTGTGTTCA ACTTTGCGAT 
GACCGAGATG TTGTTCTTGC ATCTTACCCA GCAGAAAACC TGGCTCTTTG CGTAATTTGC 
ACAGAAGACA GCAGGTTCTT TGGCCTTGTC TCGATTGATT CAACCAAAAA TATAGACACC 
GGGAGGCAGA CAAGTACCTT GAAGACATTA TGTCATGTTT TTTTCATAGA CCCAGAGCTT 
TATGAACACA AGGAGCACCA AAGCATCATC GGACACTTTG GGATTCCCTG CACCCCAGAT 
CCAGACACTC AAAGCTGTTT GGAGTTTCCT CAAACCCCCC ACTCCATTTC ACACTTCATA 
TCCGTCCTCT ATGCAGACAT GGGAGATTAC ATTGAAAAAC TAAGACTTAA ACTTGACAGT 
GAGAATCCTG ACGAAGGATC CCAAAATGCA CGGAACAGCG GCAGTGGCAG CGGCAGTGAT 
AGCGGGATTG GAAATGCTTC ACCAGAGGAA AGAGACAGTC TCATTGGTCA GTGGAGCTTC 
ACATCTCCAA ACATTTGCAA TCCACCACCA CCATATTATT CCCAGCACAG GAATAGTACA 
TTCCTCAAAC CAGAGTGCAG ATGCCTTCTT TCTGAGCCAC TAACAGGATC CGCTCAACCT 
GCGACTCCAT CAGGTGTCCC AGCCGGGGCG GACGGTTCAG CGTTTACTCC TGTTCCTCTC 
AGTAAGCAAT CTAACTTGCC TCCAAAACAA AGAGCCGGTC AACGAAGACC TTCCATTGGC 
TTAAAGGCTC GCTGGCAGAA ATCTCGCCCC AACAGCATTG AGTGCTTAGT GGAAGGGGAC 
AACGATGATC CCAAAAGTAA ATGCAGTACC CTTCCACCTG CAATGGAGCA AATCCCTACT 
CTGAGATACA AACCTTTAGA ACATACTCAG AGAATAAACT TCACCCCGCA GCTTGAGCTG 
ACATCCAGGT TCTTCAGCAG TGCAGCTAAG CAGAAGGATG GAGAAAACAA GGGATCTTTA 
TTTTGGGCTC TCACAAGGGG ACGCTCTTCT GTTCGAAGAC CGTCAGGTCC CGCTAAACGA 
CTCAGTCTGG CTCATTCACT TAATGATCTT GAGTCGGCTG CATCAGATGG AGAGCGCAGT 
GGTGGTGGTG TCCAGCGTCA CGACTGCAGC AGCGTGAGCA GTTTGGACAG TAACGGTAGT 
CTGCCCAGCG TCACCAACCA CTGTCGTTTC ACAGAGCGCC GCGTTGCATT ATGGGCAGTC 
AGCTTCGAGC GATTGCTTCA GGATCCTGTG GGTGTCCGCT ATTTTTCTGA GTTTCTAAAG 
AAGGAGTTTA GCGAGGAAAA CATCTTATTC TGGCAGGCCT GTGAATGCTT CAGCCAAGTT 
CCAGAACATG ACAAAAAGCA GCTGTCCCAG AAGGCCAAAG AGATCTACAA CCGTTTCCTC 
TCAAGTAAAG CCACAATGCC AGTGAACATC GACAGTCAAG CTCAACTTGC GGACGATGTC 
CTGACAGCTC CACAGCCTAA CATGTTCAAA CAGCCACAGC TACAGATCTT CAACCTGATG 
AAGATGGACA GTTATGCGCG CTTCTTGAAG TCCACTCTCT ATCAGGAGTG TATGCTGGCA 
GAGGTGGAGG GTCGACCTCT GCCTGACCCC TGTCCCATCC CGTGCAGCCC TGCCCCATCC 
AAACACAGCT TCACCTCTGA CCGCTCCACA TTCTCCACTC CTAAAAAGGA AAACAGGAAG 
CCCAAAACAG GCAGGTCAAG CGATGATCTC AGAGAGAAAC ACTCTGATAA GAAAAACGGC 
TTCTTTTCCT GGTACAGATA TCCAAGTATT GGGAAAGGAC AAAAGAAAAG AGAGGCCCCA 
GATTTACCCT ACCACTGTAA TGGGCGGAGA GAGTCTCAAG GTTCTCTGTC TTCTGGAGCG 
AGTCAAGAGC TCAACACTTC CTGTTCAGGA GGAAAGAATG AGCAGTGTGA GCTAAGAGGT 
TCGAGTTGTG CTTTGGATAA GGAGCGAGAG CGCTGCAGCA GGACATGTAC AGTAACACTG 
CCCGACGGCT CCAGCTGCTC TATTCCTCTC CGACAGGGAG CCTCCATCAG ACAAGTGCTG 
CTAGAGCTCT GCCAAAAACA ACACATCAAC CTGGCTGCTG TCGACCTCTT TCTCACCGGT 
GGAGAGAAGC CTTTGGTCTT GGATCAAGAT AGTATTACTC TCAGCTCCAG GGAACTTCGT 
CTTGAGAAGC GCACTTTGTT CAGATTGGAC CTGGTACCCA TTAACAGATC GGTTGGGTTG 
AAAGCGAAGC CCACAAAACC TGTGACTGAG GTTCTACGGC CTGTGGTTGC CAAGTATGGA 
CTCCAGCTTG GCGATTTGGT GGCCAGAATA AGTGGCGAGA CAGAGGTACT CGATCTTGGA 
GTGCCCATAT CAAACCTTGA TGGCCTGCGA GTTGTCTTGG AGAGGGCAGA TCCAGCCAAA 
GCAGAAAAGT CTAAAACACT CAACTCCAAA ACACCAGCTG CCAAGAAAGG GTTTCCAAGA 
GAGAGAGACG ATAACTCGAC AGTGAAACCA TTGAAAGCAG ATGTTGAAGA CAAGCATCCC 
CGCACAGCTG CGTCTGAGAG GAAGAAAGGC AAAAAGAATC ATATAGATGA AACTGAAGAG 
TTTTTCGAGT TGTTGAGTCG TGCTCAGAGC AGTCGTGTTG ACGACCAGAG AGGCCTGCTG 
AGAAAAGAAG ATCTTGTCCT TCCCGACTTC CTTCGAGTAA ACCCTGATCC CAAACCTCAA 
TCTCCTCCGT CCTGTTCAAC CCCGACCTCT CATAAACCCG GCCACACCAC TACCACCTCC 
ACAAACCCCC ATCCCCCTAA ACCCAGCCCC TGTAACGGAC ACCAAGCCTC AGCCCCGTTC 
AGCGCGCCAC TCTCCCCCAT CCTGCGTTCT GCTGGTGCCC GCGAGCAGGA GGAGGAGGAA 
GGCCTAGGAG ATTTGACTCT GGTGGCGGAG GGTGACATCA GCAGCCCAAA CTCCACCCTG 
CTCCCGCCTC CACCGCCCGT ATCACCACCG CCTTATGATG GTAGTCTAGC AGAGGCCAAC 
TTTACTCCTC CACCGGCTGT TCACGGCACA GGTACAGAAG AAGAAAACAC GAGCACTTCA 
GCAGTAAACA GCTCAACAGC ACAGCCGGAC TTGCGTGTGC GGGACAAGGG TGTCACTCTG 
GAGGACAGTT TTGAAGGATA CGCAGCTGAA CTTCGCCAGT GCCAGAACAA AATGAGAAAC 
GGCGTTTATC CCTCCACAGA AGCCCCCCGT CCCCTCAGCG GTGTGCTGGA AGTCAACGAG 
AGCGACACTG TGCAATACAA AGCAACGTTT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rgs12a ( XM_009303085.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301360.1 Danio rerio regulator of G-protein signaling 12a (rgs12a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa45979
Clone ID Related Accession (Same CDS sequence)	XM_009303085.2 , XM_009303085.3
Accession Version	XM_009303085.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	regulator of G-protein signaling 12 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303085.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTTT TGGACCGTCC CATCAGCCCG GCGGTCCGCA GGGTGGAAAT GGAGCGTGGC 
AGAGCTGGCT ATGGATTCAC CATTGCCGGT CAAGCACCAT GTGTTCTCAG AGGAATAATA 
AAAGGGAGTC CGGCGCACAG TGTAGGACTA CTACCTGGGG ATCGTGTCTT GCGTGTAAAT 
GACACAGATG TGTCTGAAGC ATCACATGAT GTTGTGGTGA AGTTGATCGG TACTTCATCA 
AAGTCACTGT GTTTGCTGGT CACATCAGGT GATCGACCAC CCATGGCCTG CTGCTCCAGT 
GACGAGGAAC TGGGTTGTCA GAATAAGAGT AAACGTCCGT GGCTCAATCC TGGAATAAAG 
GACCCTTTCC CAGGTAAACT TGATGGCAGG GATTTGAAGG AACCAATTCT TCAGTCGGTT 
GGAAGTTTTG ATACCGTCTT TGAGATCTCA GATCAGGGCA CCATGACCCC ACATCAGGAG 
AAGCAGAAAA AACAAAGACC TTTGTCTGAA CCAGATGTGT CTTACTGGTT GCAGCAGCAG 
CAGAGATCCA AAGGGAATGT CGATATTCTT TCACAGGATG AAACATCTCG AGTAGTGAGT 
GAGGATTCTG TGTTTTCAGA CCTTCAGAGT CAGAATGACC TTGCATCTGA CTCCAGCATC 
CTGAACGTTG CAATGATTGT CGGATACATT AATTCCATGG AACTGGAGCT AGTAATGTCA 
CAAACTGAGG AGGATGCATT GCAGGTCATC CGGCAATGCA TTAGTAAGAT TGGTATTGAG 
CAGAAGACTC ATTCGCTGGT CTTAATGAAG GTTAAATGCA ACTGTGTTCA ACTTTGCGAT 
GACCGAGATG TTGTTCTTGC ATCTTACCCA GCAGAAAACC TGGCTCTTTG CGTAATTTGC 
ACAGAAGACA GCAGGTTCTT TGGCCTTGTC TCGATTGATT CAACCAAAAA TATAGACACC 
GGGAGGCAGA CAAGTACCTT GAAGACATTA TGTCATGTTT TTTTCATAGA CCCAGAGCTT 
TATGAACACA AGGAGCACCA AAGCATCATC GGACACTTTG GGATTCCCTG CACCCCAGAT 
CCAGACACTC AAAGCTGTTT GGAGTTTCCT CAAACCCCCC ACTCCATTTC ACACTTCATA 
TCCGTCCTCT ATGCAGACAT GGGAGATTAC ATTGAAAAAC TAAGACTTAA ACTTGACAGT 
GAGAATCCTG ACGAAGGATC CCAAAATGCA CGGAACAGCG GCAGTGGCAG CGGCAGTGAT 
AGCGGGATTG GAAATGCTTC ACCAGAGGAA AGAGACAGTC TCATTGGTCA GTGGAGCTTC 
ACATCTCCAA ACATTTGCAA TCCACCACCA CCATATTATT CCCAGCACAG GAATAGTACA 
TTCCTCAAAC CAGAGTGCAG ATGCCTTCTT TCTGAGCCAC TAACAGGATC CGCTCAACCT 
GCGACTCCAT CAGGTGTCCC AGCCGGGGCG GACGGTTCAG CGTTTACTCC TGTTCCTCTC 
AGTAAGCAAT CTAACTTGCC TCCAAAACAA AGAGCCGGTC AACGAAGACC TTCCATTGGC 
TTAAAGGCTC GCTGGCAGAA ATCTCGCCCC AACAGCATTG AGTGCTTAGT GGAAGGGGAC 
AACGATGATC CCAAAAGTAA ATGCAGTACC CTTCCACCTG CAATGGAGCA AATCCCTACT 
CTGAGATACA AACCTTTAGA ACATACTCAG AGAATAAACT TCACCCCGCA GCTTGAGCTG 
ACATCCAGGT TCTTCAGCAG TGCAGCTAAG CAGAAGGATG GAGAAAACAA GGGATCTTTA 
TTTTGGGCTC TCACAAGGGG ACGCTCTTCT GTTCGAAGAC CGTCAGGTCC CGCTAAACGA 
CTCAGTCTGG CTCATTCACT TAATGATCTT GAGTCGGCTG CATCAGATGG AGAGCGCAGT 
GGTGGTGGTG TCCAGCGTCA CGACTGCAGC AGCGTGAGCA GTTTGGACAG TAACGGTAGT 
CTGCCCAGCG TCACCAACCA CTGTCGTTTC ACAGAGCGCC GCGTTGCATT ATGGGCAGTC 
AGCTTCGAGC GATTGCTTCA GGATCCTGTG GGTGTCCGCT ATTTTTCTGA GTTTCTAAAG 
AAGGAGTTTA GCGAGGAAAA CATCTTATTC TGGCAGGCCT GTGAATGCTT CAGCCAAGTT 
CCAGAACATG ACAAAAAGCA GCTGTCCCAG AAGGCCAAAG AGATCTACAA CCGTTTCCTC 
TCAAGTAAAG CCACAATGCC AGTGAACATC GACAGTCAAG CTCAACTTGC GGACGATGTC 
CTGACAGCTC CACAGCCTAA CATGTTCAAA CAGCCACAGC TACAGATCTT CAACCTGATG 
AAGATGGACA GTTATGCGCG CTTCTTGAAG TCCACTCTCT ATCAGGAGTG TATGCTGGCA 
GAGGTGGAGG GTCGACCTCT GCCTGACCCC TGTCCCATCC CGTGCAGCCC TGCCCCATCC 
AAACACAGCT TCACCTCTGA CCGCTCCACA TTCTCCACTC CTAAAAAGGA AAACAGGAAG 
CCCAAAACAG GCAGGTCAAG CGATGATCTC AGAGAGAAAC ACTCTGATAA GAAAAACGGC 
TTCTTTTCCT GGTACAGATA TCCAAGTATT GGGAAAGGAC AAAAGAAAAG AGAGGCCCCA 
GATTTACCCT ACCACTGTAA TGGGCGGAGA GAGTCTCAAG GTTCTCTGTC TTCTGGAGCG 
AGTCAAGAGC TCAACACTTC CTGTTCAGGA GGAAAGAATG AGCAGTGTGA GCTAAGAGGT 
TCGAGTTGTG CTTTGGATAA GGAGCGAGAG CGCTGCAGCA GGACATGTAC AGTAACACTG 
CCCGACGGCT CCAGCTGCTC TATTCCTCTC CGACAGGGAG CCTCCATCAG ACAAGTGCTG 
CTAGAGCTCT GCCAAAAACA ACACATCAAC CTGGCTGCTG TCGACCTCTT TCTCACCGGT 
GGAGAGAAGC CTTTGGTCTT GGATCAAGAT AGTATTACTC TCAGCTCCAG GGAACTTCGT 
CTTGAGAAGC GCACTTTGTT CAGATTGGAC CTGGTACCCA TTAACAGATC GGTTGGGTTG 
AAAGCGAAGC CCACAAAACC TGTGACTGAG GTTCTACGGC CTGTGGTTGC CAAGTATGGA 
CTCCAGCTTG GCGATTTGGT GGCCAGAATA AGTGGCGAGA CAGAGGTACT CGATCTTGGA 
GTGCCCATAT CAAACCTTGA TGGCCTGCGA GTTGTCTTGG AGAGGGCAGA TCCAGCCAAA 
GAAAAGTCTA AAACACTCAA CTCCAAAACA CCAGCTGCCA AGAAAGGGTT TCCAAGAGAG 
AGAGACGATA ACTCGACAGT GAAACCATTG AAAGCAGATG TTGAAGACAA GCATCCCCGC 
ACAGCTGCGT CTGAGAGGAA GAAAGGCAAA AAGAATCATA TAGATGAAAC TGAAGAGTTT 
TTCGAGTTGT TGAGTCGTGC TCAGAGCAGT CGTGTTGACG ACCAGAGAGG CCTGCTGAGA 
AAAGAAGATC TTGTCCTTCC CGACTTCCTT CGAGTAAACC CTGATCCCAA ACCTCAATCT 
CCTCCGTCCT GTTCAACCCC GACCTCTCAT AAACCCGGCC ACACCACTAC CACCTCCACA 
AACCCCCATC CCCCTAAACC CAGCCCCTGT AACGGACACC AAGCCTCAGC CCCGTTCAGC 
GCGCCACTCT CCCCCATCCT GCGTTCTGCT GGTGCCCGCG AGCAGGAGGA GGAGGAAGGC 
CTAGGAGATT TGACTCTGGT GGCGGAGGGT GACATCAGCA GCCCAAACTC CACCCTGCTC 
CCGCCTCCAC CGCCCGTATC ACCACCGCCT TATGATGGTA GTCTAGCAGA GGCCAACTTT 
ACTCCTCCAC CGGCTGTTCA CGGCACAGGT ACAGAAGAAG AAAACACGAG CACTTCAGCA 
GTAAACAGCT CAACAGCACA GCCGGACTTG CGTGTGCGGG ACAAGGGTGT CACTCTGGAG 
GACAGTTTTG AAGGATACGC AGCTGAACTT CGCCAGTGCC AGAACAAAAT GAGAAACGGC 
GTTTATCCCT CCACAGAAGC CCCCCGTCCC CTCAGCGGTG TGCTGGAAGT CAACGAGAGC 
GACACTGTGC AATACAAAGC AACGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301360.1
CDS	241..4353
Translation	MRLLDRPISPAVRRVEMERGRAGYGFTIAGQAPCVLRGIIKGSPAHSVGLLPGDRVLRVNDTDVSEASHDVVVKLIGTSSKSLCLLVTSGDRPPMACCSSDEELGCQNKSKRPWLNPGIKDPFPGKLDGRDLKEPILQSVGSFDTVFEISDQGTMTPHQEKQKKQRPLSEPDVSYWLQQQQRSKGNVDILSQDETSRVVSEDSVFSDLQSQNDLASDSSILNVAMIVGYINSMELELVMSQTEEDALQVIRQCISKIGIEQKTHSLVLMKVKCNCVQLCDDRDVVLASYPAENLALCVICTEDSRFFGLVSIDSTKNIDTGRQTSTLKTLCHVFFIDPELYEHKEHQSIIGHFGIPCTPDPDTQSCLEFPQTPHSISHFISVLYADMGDYIEKLRLKLDSENPDEGSQNARNSGSGSGSDSGIGNASPEERDSLIGQWSFTSPNICNPPPPYYSQHRNSTFLKPECRCLLSEPLTGSAQPATPSGVPAGADGSAFTPVPLSKQSNLPPKQRAGQRRPSIGLKARWQKSRPNSIECLVEGDNDDPKSKCSTLPPAMEQIPTLRYKPLEHTQRINFTPQLELTSRFFSSAAKQKDGENKGSLFWALTRGRSSVRRPSGPAKRLSLAHSLNDLESAASDGERSGGGVQRHDCSSVSSLDSNGSLPSVTNHCRFTERRVALWAVSFERLLQDPVGVRYFSEFLKKEFSEENILFWQACECFSQVPEHDKKQLSQKAKEIYNRFLSSKATMPVNIDSQAQLADDVLTAPQPNMFKQPQLQIFNLMKMDSYARFLKSTLYQECMLAEVEGRPLPDPCPIPCSPAPSKHSFTSDRSTFSTPKKENRKPKTGRSSDDLREKHSDKKNGFFSWYRYPSIGKGQKKREAPDLPYHCNGRRESQGSLSSGASQELNTSCSGGKNEQCELRGSSCALDKERERCSRTCTVTLPDGSSCSIPLRQGASIRQVLLELCQKQHINLAAVDLFLTGGEKPLVLDQDSITLSSRELRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLQLGDLVARISGETEVLDLGVPISNLDGLRVVLERADPAKEKSKTLNSKTPAAKKGFPRERDDNSTVKPLKADVEDKHPRTAASERKKGKKNHIDETEEFFELLSRAQSSRVDDQRGLLRKEDLVLPDFLRVNPDPKPQSPPSCSTPTSHKPGHTTTTSTNPHPPKPSPCNGHQASAPFSAPLSPILRSAGAREQEEEEGLGDLTLVAEGDISSPNSTLLPPPPPVSPPPYDGSLAEANFTPPPAVHGTGTEEENTSTSAVNSSTAQPDLRVRDKGVTLEDSFEGYAAELRQCQNKMRNGVYPSTEAPRPLSGVLEVNESDTVQYKATFV

Target ORF information:

RefSeq Version	XM_009303085.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio regulator of G-protein signaling 12a (rgs12a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303085.2

ORF Insert Sequence:

ATGAGGCTTT TGGACCGTCC CATCAGCCCG GCGGTCCGCA GGGTGGAAAT GGAGCGTGGC 
AGAGCTGGCT ATGGATTCAC CATTGCCGGT CAAGCACCAT GTGTTCTCAG AGGAATAATA 
AAAGGGAGTC CGGCGCACAG TGTAGGACTA CTACCTGGGG ATCGTGTCTT GCGTGTAAAT 
GACACAGATG TGTCTGAAGC ATCACATGAT GTTGTGGTGA AGTTGATCGG TACTTCATCA 
AAGTCACTGT GTTTGCTGGT CACATCAGGT GATCGACCAC CCATGGCCTG CTGCTCCAGT 
GACGAGGAAC TGGGTTGTCA GAATAAGAGT AAACGTCCGT GGCTCAATCC TGGAATAAAG 
GACCCTTTCC CAGGTAAACT TGATGGCAGG GATTTGAAGG AACCAATTCT TCAGTCGGTT 
GGAAGTTTTG ATACCGTCTT TGAGATCTCA GATCAGGGCA CCATGACCCC ACATCAGGAG 
AAGCAGAAAA AACAAAGACC TTTGTCTGAA CCAGATGTGT CTTACTGGTT GCAGCAGCAG 
CAGAGATCCA AAGGGAATGT CGATATTCTT TCACAGGATG AAACATCTCG AGTAGTGAGT 
GAGGATTCTG TGTTTTCAGA CCTTCAGAGT CAGAATGACC TTGCATCTGA CTCCAGCATC 
CTGAACGTTG CAATGATTGT CGGATACATT AATTCCATGG AACTGGAGCT AGTAATGTCA 
CAAACTGAGG AGGATGCATT GCAGGTCATC CGGCAATGCA TTAGTAAGAT TGGTATTGAG 
CAGAAGACTC ATTCGCTGGT CTTAATGAAG GTTAAATGCA ACTGTGTTCA ACTTTGCGAT 
GACCGAGATG TTGTTCTTGC ATCTTACCCA GCAGAAAACC TGGCTCTTTG CGTAATTTGC 
ACAGAAGACA GCAGGTTCTT TGGCCTTGTC TCGATTGATT CAACCAAAAA TATAGACACC 
GGGAGGCAGA CAAGTACCTT GAAGACATTA TGTCATGTTT TTTTCATAGA CCCAGAGCTT 
TATGAACACA AGGAGCACCA AAGCATCATC GGACACTTTG GGATTCCCTG CACCCCAGAT 
CCAGACACTC AAAGCTGTTT GGAGTTTCCT CAAACCCCCC ACTCCATTTC ACACTTCATA 
TCCGTCCTCT ATGCAGACAT GGGAGATTAC ATTGAAAAAC TAAGACTTAA ACTTGACAGT 
GAGAATCCTG ACGAAGGATC CCAAAATGCA CGGAACAGCG GCAGTGGCAG CGGCAGTGAT 
AGCGGGATTG GAAATGCTTC ACCAGAGGAA AGAGACAGTC TCATTGGTCA GTGGAGCTTC 
ACATCTCCAA ACATTTGCAA TCCACCACCA CCATATTATT CCCAGCACAG GAATAGTACA 
TTCCTCAAAC CAGAGTGCAG ATGCCTTCTT TCTGAGCCAC TAACAGGATC CGCTCAACCT 
GCGACTCCAT CAGGTGTCCC AGCCGGGGCG GACGGTTCAG CGTTTACTCC TGTTCCTCTC 
AGTAAGCAAT CTAACTTGCC TCCAAAACAA AGAGCCGGTC AACGAAGACC TTCCATTGGC 
TTAAAGGCTC GCTGGCAGAA ATCTCGCCCC AACAGCATTG AGTGCTTAGT GGAAGGGGAC 
AACGATGATC CCAAAAGTAA ATGCAGTACC CTTCCACCTG CAATGGAGCA AATCCCTACT 
CTGAGATACA AACCTTTAGA ACATACTCAG AGAATAAACT TCACCCCGCA GCTTGAGCTG 
ACATCCAGGT TCTTCAGCAG TGCAGCTAAG CAGAAGGATG GAGAAAACAA GGGATCTTTA 
TTTTGGGCTC TCACAAGGGG ACGCTCTTCT GTTCGAAGAC CGTCAGGTCC CGCTAAACGA 
CTCAGTCTGG CTCATTCACT TAATGATCTT GAGTCGGCTG CATCAGATGG AGAGCGCAGT 
GGTGGTGGTG TCCAGCGTCA CGACTGCAGC AGCGTGAGCA GTTTGGACAG TAACGGTAGT 
CTGCCCAGCG TCACCAACCA CTGTCGTTTC ACAGAGCGCC GCGTTGCATT ATGGGCAGTC 
AGCTTCGAGC GATTGCTTCA GGATCCTGTG GGTGTCCGCT ATTTTTCTGA GTTTCTAAAG 
AAGGAGTTTA GCGAGGAAAA CATCTTATTC TGGCAGGCCT GTGAATGCTT CAGCCAAGTT 
CCAGAACATG ACAAAAAGCA GCTGTCCCAG AAGGCCAAAG AGATCTACAA CCGTTTCCTC 
TCAAGTAAAG CCACAATGCC AGTGAACATC GACAGTCAAG CTCAACTTGC GGACGATGTC 
CTGACAGCTC CACAGCCTAA CATGTTCAAA CAGCCACAGC TACAGATCTT CAACCTGATG 
AAGATGGACA GTTATGCGCG CTTCTTGAAG TCCACTCTCT ATCAGGAGTG TATGCTGGCA 
GAGGTGGAGG GTCGACCTCT GCCTGACCCC TGTCCCATCC CGTGCAGCCC TGCCCCATCC 
AAACACAGCT TCACCTCTGA CCGCTCCACA TTCTCCACTC CTAAAAAGGA AAACAGGAAG 
CCCAAAACAG GCAGGTCAAG CGATGATCTC AGAGAGAAAC ACTCTGATAA GAAAAACGGC 
TTCTTTTCCT GGTACAGATA TCCAAGTATT GGGAAAGGAC AAAAGAAAAG AGAGGCCCCA 
GATTTACCCT ACCACTGTAA TGGGCGGAGA GAGTCTCAAG GTTCTCTGTC TTCTGGAGCG 
AGTCAAGAGC TCAACACTTC CTGTTCAGGA GGAAAGAATG AGCAGTGTGA GCTAAGAGGT 
TCGAGTTGTG CTTTGGATAA GGAGCGAGAG CGCTGCAGCA GGACATGTAC AGTAACACTG 
CCCGACGGCT CCAGCTGCTC TATTCCTCTC CGACAGGGAG CCTCCATCAG ACAAGTGCTG 
CTAGAGCTCT GCCAAAAACA ACACATCAAC CTGGCTGCTG TCGACCTCTT TCTCACCGGT 
GGAGAGAAGC CTTTGGTCTT GGATCAAGAT AGTATTACTC TCAGCTCCAG GGAACTTCGT 
CTTGAGAAGC GCACTTTGTT CAGATTGGAC CTGGTACCCA TTAACAGATC GGTTGGGTTG 
AAAGCGAAGC CCACAAAACC TGTGACTGAG GTTCTACGGC CTGTGGTTGC CAAGTATGGA 
CTCCAGCTTG GCGATTTGGT GGCCAGAATA AGTGGCGAGA CAGAGGTACT CGATCTTGGA 
GTGCCCATAT CAAACCTTGA TGGCCTGCGA GTTGTCTTGG AGAGGGCAGA TCCAGCCAAA 
GAAAAGTCTA AAACACTCAA CTCCAAAACA CCAGCTGCCA AGAAAGGGTT TCCAAGAGAG 
AGAGACGATA ACTCGACAGT GAAACCATTG AAAGCAGATG TTGAAGACAA GCATCCCCGC 
ACAGCTGCGT CTGAGAGGAA GAAAGGCAAA AAGAATCATA TAGATGAAAC TGAAGAGTTT 
TTCGAGTTGT TGAGTCGTGC TCAGAGCAGT CGTGTTGACG ACCAGAGAGG CCTGCTGAGA 
AAAGAAGATC TTGTCCTTCC CGACTTCCTT CGAGTAAACC CTGATCCCAA ACCTCAATCT 
CCTCCGTCCT GTTCAACCCC GACCTCTCAT AAACCCGGCC ACACCACTAC CACCTCCACA 
AACCCCCATC CCCCTAAACC CAGCCCCTGT AACGGACACC AAGCCTCAGC CCCGTTCAGC 
GCGCCACTCT CCCCCATCCT GCGTTCTGCT GGTGCCCGCG AGCAGGAGGA GGAGGAAGGC 
CTAGGAGATT TGACTCTGGT GGCGGAGGGT GACATCAGCA GCCCAAACTC CACCCTGCTC 
CCGCCTCCAC CGCCCGTATC ACCACCGCCT TATGATGGTA GTCTAGCAGA GGCCAACTTT 
ACTCCTCCAC CGGCTGTTCA CGGCACAGGT ACAGAAGAAG AAAACACGAG CACTTCAGCA 
GTAAACAGCT CAACAGCACA GCCGGACTTG CGTGTGCGGG ACAAGGGTGT CACTCTGGAG 
GACAGTTTTG AAGGATACGC AGCTGAACTT CGCCAGTGCC AGAACAAAAT GAGAAACGGC 
GTTTATCCCT CCACAGAAGC CCCCCGTCCC CTCAGCGGTG TGCTGGAAGT CAACGAGAGC 
GACACTGTGC AATACAAAGC AACGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rgs12a ( XM_009303085.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301360.1 Danio rerio regulator of G protein signaling 12a (rgs12a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa45979
Clone ID Related Accession (Same CDS sequence)	XM_009303085.2 , XM_009303085.3
Accession Version	XM_009303085.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	regulator of G-protein signaling 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303085.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTTT TGGACCGTCC CATCAGCCCG GCGGTCCGCA GGGTGGAAAT GGAGCGTGGC 
AGAGCTGGCT ATGGATTCAC CATTGCCGGT CAAGCACCAT GTGTTCTCAG AGGAATAATA 
AAAGGGAGTC CGGCGCACAG TGTAGGACTA CTACCTGGGG ATCGTGTCTT GCGTGTAAAT 
GACACAGATG TGTCTGAAGC ATCACATGAT GTTGTGGTGA AGTTGATCGG TACTTCATCA 
AAGTCACTGT GTTTGCTGGT CACATCAGGT GATCGACCAC CCATGGCCTG CTGCTCCAGT 
GACGAGGAAC TGGGTTGTCA GAATAAGAGT AAACGTCCGT GGCTCAATCC TGGAATAAAG 
GACCCTTTCC CAGGTAAACT TGATGGCAGG GATTTGAAGG AACCAATTCT TCAGTCGGTT 
GGAAGTTTTG ATACCGTCTT TGAGATCTCA GATCAGGGCA CCATGACCCC ACATCAGGAG 
AAGCAGAAAA AACAAAGACC TTTGTCTGAA CCAGATGTGT CTTACTGGTT GCAGCAGCAG 
CAGAGATCCA AAGGGAATGT CGATATTCTT TCACAGGATG AAACATCTCG AGTAGTGAGT 
GAGGATTCTG TGTTTTCAGA CCTTCAGAGT CAGAATGACC TTGCATCTGA CTCCAGCATC 
CTGAACGTTG CAATGATTGT CGGATACATT AATTCCATGG AACTGGAGCT AGTAATGTCA 
CAAACTGAGG AGGATGCATT GCAGGTCATC CGGCAATGCA TTAGTAAGAT TGGTATTGAG 
CAGAAGACTC ATTCGCTGGT CTTAATGAAG GTTAAATGCA ACTGTGTTCA ACTTTGCGAT 
GACCGAGATG TTGTTCTTGC ATCTTACCCA GCAGAAAACC TGGCTCTTTG CGTAATTTGC 
ACAGAAGACA GCAGGTTCTT TGGCCTTGTC TCGATTGATT CAACCAAAAA TATAGACACC 
GGGAGGCAGA CAAGTACCTT GAAGACATTA TGTCATGTTT TTTTCATAGA CCCAGAGCTT 
TATGAACACA AGGAGCACCA AAGCATCATC GGACACTTTG GGATTCCCTG CACCCCAGAT 
CCAGACACTC AAAGCTGTTT GGAGTTTCCT CAAACCCCCC ACTCCATTTC ACACTTCATA 
TCCGTCCTCT ATGCAGACAT GGGAGATTAC ATTGAAAAAC TAAGACTTAA ACTTGACAGT 
GAGAATCCTG ACGAAGGATC CCAAAATGCA CGGAACAGCG GCAGTGGCAG CGGCAGTGAT 
AGCGGGATTG GAAATGCTTC ACCAGAGGAA AGAGACAGTC TCATTGGTCA GTGGAGCTTC 
ACATCTCCAA ACATTTGCAA TCCACCACCA CCATATTATT CCCAGCACAG GAATAGTACA 
TTCCTCAAAC CAGAGTGCAG ATGCCTTCTT TCTGAGCCAC TAACAGGATC CGCTCAACCT 
GCGACTCCAT CAGGTGTCCC AGCCGGGGCG GACGGTTCAG CGTTTACTCC TGTTCCTCTC 
AGTAAGCAAT CTAACTTGCC TCCAAAACAA AGAGCCGGTC AACGAAGACC TTCCATTGGC 
TTAAAGGCTC GCTGGCAGAA ATCTCGCCCC AACAGCATTG AGTGCTTAGT GGAAGGGGAC 
AACGATGATC CCAAAAGTAA ATGCAGTACC CTTCCACCTG CAATGGAGCA AATCCCTACT 
CTGAGATACA AACCTTTAGA ACATACTCAG AGAATAAACT TCACCCCGCA GCTTGAGCTG 
ACATCCAGGT TCTTCAGCAG TGCAGCTAAG CAGAAGGATG GAGAAAACAA GGGATCTTTA 
TTTTGGGCTC TCACAAGGGG ACGCTCTTCT GTTCGAAGAC CGTCAGGTCC CGCTAAACGA 
CTCAGTCTGG CTCATTCACT TAATGATCTT GAGTCGGCTG CATCAGATGG AGAGCGCAGT 
GGTGGTGGTG TCCAGCGTCA CGACTGCAGC AGCGTGAGCA GTTTGGACAG TAACGGTAGT 
CTGCCCAGCG TCACCAACCA CTGTCGTTTC ACAGAGCGCC GCGTTGCATT ATGGGCAGTC 
AGCTTCGAGC GATTGCTTCA GGATCCTGTG GGTGTCCGCT ATTTTTCTGA GTTTCTAAAG 
AAGGAGTTTA GCGAGGAAAA CATCTTATTC TGGCAGGCCT GTGAATGCTT CAGCCAAGTT 
CCAGAACATG ACAAAAAGCA GCTGTCCCAG AAGGCCAAAG AGATCTACAA CCGTTTCCTC 
TCAAGTAAAG CCACAATGCC AGTGAACATC GACAGTCAAG CTCAACTTGC GGACGATGTC 
CTGACAGCTC CACAGCCTAA CATGTTCAAA CAGCCACAGC TACAGATCTT CAACCTGATG 
AAGATGGACA GTTATGCGCG CTTCTTGAAG TCCACTCTCT ATCAGGAGTG TATGCTGGCA 
GAGGTGGAGG GTCGACCTCT GCCTGACCCC TGTCCCATCC CGTGCAGCCC TGCCCCATCC 
AAACACAGCT TCACCTCTGA CCGCTCCACA TTCTCCACTC CTAAAAAGGA AAACAGGAAG 
CCCAAAACAG GCAGGTCAAG CGATGATCTC AGAGAGAAAC ACTCTGATAA GAAAAACGGC 
TTCTTTTCCT GGTACAGATA TCCAAGTATT GGGAAAGGAC AAAAGAAAAG AGAGGCCCCA 
GATTTACCCT ACCACTGTAA TGGGCGGAGA GAGTCTCAAG GTTCTCTGTC TTCTGGAGCG 
AGTCAAGAGC TCAACACTTC CTGTTCAGGA GGAAAGAATG AGCAGTGTGA GCTAAGAGGT 
TCGAGTTGTG CTTTGGATAA GGAGCGAGAG CGCTGCAGCA GGACATGTAC AGTAACACTG 
CCCGACGGCT CCAGCTGCTC TATTCCTCTC CGACAGGGAG CCTCCATCAG ACAAGTGCTG 
CTAGAGCTCT GCCAAAAACA ACACATCAAC CTGGCTGCTG TCGACCTCTT TCTCACCGGT 
GGAGAGAAGC CTTTGGTCTT GGATCAAGAT AGTATTACTC TCAGCTCCAG GGAACTTCGT 
CTTGAGAAGC GCACTTTGTT CAGATTGGAC CTGGTACCCA TTAACAGATC GGTTGGGTTG 
AAAGCGAAGC CCACAAAACC TGTGACTGAG GTTCTACGGC CTGTGGTTGC CAAGTATGGA 
CTCCAGCTTG GCGATTTGGT GGCCAGAATA AGTGGCGAGA CAGAGGTACT CGATCTTGGA 
GTGCCCATAT CAAACCTTGA TGGCCTGCGA GTTGTCTTGG AGAGGGCAGA TCCAGCCAAA 
GAAAAGTCTA AAACACTCAA CTCCAAAACA CCAGCTGCCA AGAAAGGGTT TCCAAGAGAG 
AGAGACGATA ACTCGACAGT GAAACCATTG AAAGCAGATG TTGAAGACAA GCATCCCCGC 
ACAGCTGCGT CTGAGAGGAA GAAAGGCAAA AAGAATCATA TAGATGAAAC TGAAGAGTTT 
TTCGAGTTGT TGAGTCGTGC TCAGAGCAGT CGTGTTGACG ACCAGAGAGG CCTGCTGAGA 
AAAGAAGATC TTGTCCTTCC CGACTTCCTT CGAGTAAACC CTGATCCCAA ACCTCAATCT 
CCTCCGTCCT GTTCAACCCC GACCTCTCAT AAACCCGGCC ACACCACTAC CACCTCCACA 
AACCCCCATC CCCCTAAACC CAGCCCCTGT AACGGACACC AAGCCTCAGC CCCGTTCAGC 
GCGCCACTCT CCCCCATCCT GCGTTCTGCT GGTGCCCGCG AGCAGGAGGA GGAGGAAGGC 
CTAGGAGATT TGACTCTGGT GGCGGAGGGT GACATCAGCA GCCCAAACTC CACCCTGCTC 
CCGCCTCCAC CGCCCGTATC ACCACCGCCT TATGATGGTA GTCTAGCAGA GGCCAACTTT 
ACTCCTCCAC CGGCTGTTCA CGGCACAGGT ACAGAAGAAG AAAACACGAG CACTTCAGCA 
GTAAACAGCT CAACAGCACA GCCGGACTTG CGTGTGCGGG ACAAGGGTGT CACTCTGGAG 
GACAGTTTTG AAGGATACGC AGCTGAACTT CGCCAGTGCC AGAACAAAAT GAGAAACGGC 
GTTTATCCCT CCACAGAAGC CCCCCGTCCC CTCAGCGGTG TGCTGGAAGT CAACGAGAGC 
GACACTGTGC AATACAAAGC AACGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301360.1
CDS	263..4375
Translation	MRLLDRPISPAVRRVEMERGRAGYGFTIAGQAPCVLRGIIKGSPAHSVGLLPGDRVLRVNDTDVSEASHDVVVKLIGTSSKSLCLLVTSGDRPPMACCSSDEELGCQNKSKRPWLNPGIKDPFPGKLDGRDLKEPILQSVGSFDTVFEISDQGTMTPHQEKQKKQRPLSEPDVSYWLQQQQRSKGNVDILSQDETSRVVSEDSVFSDLQSQNDLASDSSILNVAMIVGYINSMELELVMSQTEEDALQVIRQCISKIGIEQKTHSLVLMKVKCNCVQLCDDRDVVLASYPAENLALCVICTEDSRFFGLVSIDSTKNIDTGRQTSTLKTLCHVFFIDPELYEHKEHQSIIGHFGIPCTPDPDTQSCLEFPQTPHSISHFISVLYADMGDYIEKLRLKLDSENPDEGSQNARNSGSGSGSDSGIGNASPEERDSLIGQWSFTSPNICNPPPPYYSQHRNSTFLKPECRCLLSEPLTGSAQPATPSGVPAGADGSAFTPVPLSKQSNLPPKQRAGQRRPSIGLKARWQKSRPNSIECLVEGDNDDPKSKCSTLPPAMEQIPTLRYKPLEHTQRINFTPQLELTSRFFSSAAKQKDGENKGSLFWALTRGRSSVRRPSGPAKRLSLAHSLNDLESAASDGERSGGGVQRHDCSSVSSLDSNGSLPSVTNHCRFTERRVALWAVSFERLLQDPVGVRYFSEFLKKEFSEENILFWQACECFSQVPEHDKKQLSQKAKEIYNRFLSSKATMPVNIDSQAQLADDVLTAPQPNMFKQPQLQIFNLMKMDSYARFLKSTLYQECMLAEVEGRPLPDPCPIPCSPAPSKHSFTSDRSTFSTPKKENRKPKTGRSSDDLREKHSDKKNGFFSWYRYPSIGKGQKKREAPDLPYHCNGRRESQGSLSSGASQELNTSCSGGKNEQCELRGSSCALDKERERCSRTCTVTLPDGSSCSIPLRQGASIRQVLLELCQKQHINLAAVDLFLTGGEKPLVLDQDSITLSSRELRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLQLGDLVARISGETEVLDLGVPISNLDGLRVVLERADPAKEKSKTLNSKTPAAKKGFPRERDDNSTVKPLKADVEDKHPRTAASERKKGKKNHIDETEEFFELLSRAQSSRVDDQRGLLRKEDLVLPDFLRVNPDPKPQSPPSCSTPTSHKPGHTTTTSTNPHPPKPSPCNGHQASAPFSAPLSPILRSAGAREQEEEEGLGDLTLVAEGDISSPNSTLLPPPPPVSPPPYDGSLAEANFTPPPAVHGTGTEEENTSTSAVNSSTAQPDLRVRDKGVTLEDSFEGYAAELRQCQNKMRNGVYPSTEAPRPLSGVLEVNESDTVQYKATFV

Target ORF information:

RefSeq Version	XM_009303085.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio regulator of G protein signaling 12a (rgs12a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303085.3

ORF Insert Sequence:

ATGAGGCTTT TGGACCGTCC CATCAGCCCG GCGGTCCGCA GGGTGGAAAT GGAGCGTGGC 
AGAGCTGGCT ATGGATTCAC CATTGCCGGT CAAGCACCAT GTGTTCTCAG AGGAATAATA 
AAAGGGAGTC CGGCGCACAG TGTAGGACTA CTACCTGGGG ATCGTGTCTT GCGTGTAAAT 
GACACAGATG TGTCTGAAGC ATCACATGAT GTTGTGGTGA AGTTGATCGG TACTTCATCA 
AAGTCACTGT GTTTGCTGGT CACATCAGGT GATCGACCAC CCATGGCCTG CTGCTCCAGT 
GACGAGGAAC TGGGTTGTCA GAATAAGAGT AAACGTCCGT GGCTCAATCC TGGAATAAAG 
GACCCTTTCC CAGGTAAACT TGATGGCAGG GATTTGAAGG AACCAATTCT TCAGTCGGTT 
GGAAGTTTTG ATACCGTCTT TGAGATCTCA GATCAGGGCA CCATGACCCC ACATCAGGAG 
AAGCAGAAAA AACAAAGACC TTTGTCTGAA CCAGATGTGT CTTACTGGTT GCAGCAGCAG 
CAGAGATCCA AAGGGAATGT CGATATTCTT TCACAGGATG AAACATCTCG AGTAGTGAGT 
GAGGATTCTG TGTTTTCAGA CCTTCAGAGT CAGAATGACC TTGCATCTGA CTCCAGCATC 
CTGAACGTTG CAATGATTGT CGGATACATT AATTCCATGG AACTGGAGCT AGTAATGTCA 
CAAACTGAGG AGGATGCATT GCAGGTCATC CGGCAATGCA TTAGTAAGAT TGGTATTGAG 
CAGAAGACTC ATTCGCTGGT CTTAATGAAG GTTAAATGCA ACTGTGTTCA ACTTTGCGAT 
GACCGAGATG TTGTTCTTGC ATCTTACCCA GCAGAAAACC TGGCTCTTTG CGTAATTTGC 
ACAGAAGACA GCAGGTTCTT TGGCCTTGTC TCGATTGATT CAACCAAAAA TATAGACACC 
GGGAGGCAGA CAAGTACCTT GAAGACATTA TGTCATGTTT TTTTCATAGA CCCAGAGCTT 
TATGAACACA AGGAGCACCA AAGCATCATC GGACACTTTG GGATTCCCTG CACCCCAGAT 
CCAGACACTC AAAGCTGTTT GGAGTTTCCT CAAACCCCCC ACTCCATTTC ACACTTCATA 
TCCGTCCTCT ATGCAGACAT GGGAGATTAC ATTGAAAAAC TAAGACTTAA ACTTGACAGT 
GAGAATCCTG ACGAAGGATC CCAAAATGCA CGGAACAGCG GCAGTGGCAG CGGCAGTGAT 
AGCGGGATTG GAAATGCTTC ACCAGAGGAA AGAGACAGTC TCATTGGTCA GTGGAGCTTC 
ACATCTCCAA ACATTTGCAA TCCACCACCA CCATATTATT CCCAGCACAG GAATAGTACA 
TTCCTCAAAC CAGAGTGCAG ATGCCTTCTT TCTGAGCCAC TAACAGGATC CGCTCAACCT 
GCGACTCCAT CAGGTGTCCC AGCCGGGGCG GACGGTTCAG CGTTTACTCC TGTTCCTCTC 
AGTAAGCAAT CTAACTTGCC TCCAAAACAA AGAGCCGGTC AACGAAGACC TTCCATTGGC 
TTAAAGGCTC GCTGGCAGAA ATCTCGCCCC AACAGCATTG AGTGCTTAGT GGAAGGGGAC 
AACGATGATC CCAAAAGTAA ATGCAGTACC CTTCCACCTG CAATGGAGCA AATCCCTACT 
CTGAGATACA AACCTTTAGA ACATACTCAG AGAATAAACT TCACCCCGCA GCTTGAGCTG 
ACATCCAGGT TCTTCAGCAG TGCAGCTAAG CAGAAGGATG GAGAAAACAA GGGATCTTTA 
TTTTGGGCTC TCACAAGGGG ACGCTCTTCT GTTCGAAGAC CGTCAGGTCC CGCTAAACGA 
CTCAGTCTGG CTCATTCACT TAATGATCTT GAGTCGGCTG CATCAGATGG AGAGCGCAGT 
GGTGGTGGTG TCCAGCGTCA CGACTGCAGC AGCGTGAGCA GTTTGGACAG TAACGGTAGT 
CTGCCCAGCG TCACCAACCA CTGTCGTTTC ACAGAGCGCC GCGTTGCATT ATGGGCAGTC 
AGCTTCGAGC GATTGCTTCA GGATCCTGTG GGTGTCCGCT ATTTTTCTGA GTTTCTAAAG 
AAGGAGTTTA GCGAGGAAAA CATCTTATTC TGGCAGGCCT GTGAATGCTT CAGCCAAGTT 
CCAGAACATG ACAAAAAGCA GCTGTCCCAG AAGGCCAAAG AGATCTACAA CCGTTTCCTC 
TCAAGTAAAG CCACAATGCC AGTGAACATC GACAGTCAAG CTCAACTTGC GGACGATGTC 
CTGACAGCTC CACAGCCTAA CATGTTCAAA CAGCCACAGC TACAGATCTT CAACCTGATG 
AAGATGGACA GTTATGCGCG CTTCTTGAAG TCCACTCTCT ATCAGGAGTG TATGCTGGCA 
GAGGTGGAGG GTCGACCTCT GCCTGACCCC TGTCCCATCC CGTGCAGCCC TGCCCCATCC 
AAACACAGCT TCACCTCTGA CCGCTCCACA TTCTCCACTC CTAAAAAGGA AAACAGGAAG 
CCCAAAACAG GCAGGTCAAG CGATGATCTC AGAGAGAAAC ACTCTGATAA GAAAAACGGC 
TTCTTTTCCT GGTACAGATA TCCAAGTATT GGGAAAGGAC AAAAGAAAAG AGAGGCCCCA 
GATTTACCCT ACCACTGTAA TGGGCGGAGA GAGTCTCAAG GTTCTCTGTC TTCTGGAGCG 
AGTCAAGAGC TCAACACTTC CTGTTCAGGA GGAAAGAATG AGCAGTGTGA GCTAAGAGGT 
TCGAGTTGTG CTTTGGATAA GGAGCGAGAG CGCTGCAGCA GGACATGTAC AGTAACACTG 
CCCGACGGCT CCAGCTGCTC TATTCCTCTC CGACAGGGAG CCTCCATCAG ACAAGTGCTG 
CTAGAGCTCT GCCAAAAACA ACACATCAAC CTGGCTGCTG TCGACCTCTT TCTCACCGGT 
GGAGAGAAGC CTTTGGTCTT GGATCAAGAT AGTATTACTC TCAGCTCCAG GGAACTTCGT 
CTTGAGAAGC GCACTTTGTT CAGATTGGAC CTGGTACCCA TTAACAGATC GGTTGGGTTG 
AAAGCGAAGC CCACAAAACC TGTGACTGAG GTTCTACGGC CTGTGGTTGC CAAGTATGGA 
CTCCAGCTTG GCGATTTGGT GGCCAGAATA AGTGGCGAGA CAGAGGTACT CGATCTTGGA 
GTGCCCATAT CAAACCTTGA TGGCCTGCGA GTTGTCTTGG AGAGGGCAGA TCCAGCCAAA 
GAAAAGTCTA AAACACTCAA CTCCAAAACA CCAGCTGCCA AGAAAGGGTT TCCAAGAGAG 
AGAGACGATA ACTCGACAGT GAAACCATTG AAAGCAGATG TTGAAGACAA GCATCCCCGC 
ACAGCTGCGT CTGAGAGGAA GAAAGGCAAA AAGAATCATA TAGATGAAAC TGAAGAGTTT 
TTCGAGTTGT TGAGTCGTGC TCAGAGCAGT CGTGTTGACG ACCAGAGAGG CCTGCTGAGA 
AAAGAAGATC TTGTCCTTCC CGACTTCCTT CGAGTAAACC CTGATCCCAA ACCTCAATCT 
CCTCCGTCCT GTTCAACCCC GACCTCTCAT AAACCCGGCC ACACCACTAC CACCTCCACA 
AACCCCCATC CCCCTAAACC CAGCCCCTGT AACGGACACC AAGCCTCAGC CCCGTTCAGC 
GCGCCACTCT CCCCCATCCT GCGTTCTGCT GGTGCCCGCG AGCAGGAGGA GGAGGAAGGC 
CTAGGAGATT TGACTCTGGT GGCGGAGGGT GACATCAGCA GCCCAAACTC CACCCTGCTC 
CCGCCTCCAC CGCCCGTATC ACCACCGCCT TATGATGGTA GTCTAGCAGA GGCCAACTTT 
ACTCCTCCAC CGGCTGTTCA CGGCACAGGT ACAGAAGAAG AAAACACGAG CACTTCAGCA 
GTAAACAGCT CAACAGCACA GCCGGACTTG CGTGTGCGGG ACAAGGGTGT CACTCTGGAG 
GACAGTTTTG AAGGATACGC AGCTGAACTT CGCCAGTGCC AGAACAAAAT GAGAAACGGC 
GTTTATCCCT CCACAGAAGC CCCCCGTCCC CTCAGCGGTG TGCTGGAAGT CAACGAGAGC 
GACACTGTGC AATACAAAGC AACGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rgs12a ( XM_009303086.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301361.1 Danio rerio regulator of G-protein signaling 12a (rgs12a), transcript variant X3, mRNA.

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CloneID	ODa45980
Clone ID Related Accession (Same CDS sequence)	XM_009303086.2 , XM_009303086.3
Accession Version	XM_009303086.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	regulator of G-protein signaling 12 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303086.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTTT TGGACCGTCC CATCAGCCCG GCGGTCCGCA GGGTGGAAAT GGAGCGTGGC 
AGAGCTGGCT ATGGATTCAC CATTGCCGGT CAAGCACCAT GTGTTCTCAG AGGAATAATA 
AAAGGGAGTC CGGCGCACAG TGTAGGACTA CTACCTGGGG ATCGTGTCTT GCGTGTAAAT 
GACACAGATG TGTCTGAAGC ATCACATGAT GTTGTGGTGA AGTTGATCGG TACTTCATCA 
AAGTCACTGT GTTTGCTGGT CACATCAGGT GATCGACCAC CCATGGCCTG CTGCTCCAGT 
GACGAGGAAC TGGGTTGTCA GAATAAGAGT AAACGTCCGT GGCTCAATCC TGGAATAAAG 
GACCCTTTCC CAGGTAAACT TGATGGCAGG GATTTGAAGG AACCAATTCT TCAGTCGGTT 
GGAAGTTTTG ATACCGTCTT TGAGATCTCA GATCAGGGCA CCATGACCCC ACATCAGGAG 
AAGCAGAAAA AACAAAGACC TTTGTCTGAA CCAGATGTGT CTTACTGGTT GCAGCAGCAG 
CAGAGATCCA AAGGGAATGT CGATATTCTT TCACAGGATG AAACATCTCG AGTAGTGAGT 
GAGGATTCTG TGTTTTCAGA CCTTCAGAGT CAGAATGACC TTGCATCTGA CTCCAGCATC 
CTGAACGTTG CAATGATTGT CGGATACATT AATTCCATGG AACTGGAGCT AGTAATGTCA 
CAAACTGAGG AGGATGCATT GCAGGTCATC CGGCAATGCA TTAGTAAGAT TGGTATTGAG 
CAGAAGACTC ATTCGCTGGT CTTAATGAAG GTTAAATGCA ACTGTGTTCA ACTTTGCGAT 
GACCGAGATG TTGTTCTTGC ATCTTACCCA GCAGAAAACC TGGCTCTTTG CGTAATTTGC 
ACAGAAGACA GCAGGTTCTT TGGCCTTGTC TCGATTGATT CAACCAAAAA TATAGACACC 
GGGAGGCAGA CAAGTACCTT GAAGACATTA TGTCATGTTT TTTTCATAGA CCCAGAGCTT 
TATGAACACA AGGAGCACCA AAGCATCATC GGACACTTTG GGATTCCCTG CACCCCAGAT 
CCAGACACTC AAAGCTGTTT GGAGTTTCCT CAAACCCCCC ACTCCATTTC ACACTTCATA 
TCCGTCCTCT ATGCAGACAT GGGAGATTAC ATTGAAAAAC TAAGACTTAA ACTTGACAGT 
GAGAATCCTG ACGAAGGATC CCAAAATGCA CGGAACAGCG GCAGTGGCAG CGGCAGTGAT 
AGCGGGATTG GAAATGCTTC ACCAGAGGAA AGAGACAGTC TCATTGGTCA GTGGAGCTTC 
ACATCTCCAA ACATTTGCAA TCCACCACCA CCATATTATT CCCAGCACAG GAATAGTACA 
TTCCTCAAAC CAGAGTGCAG ATGCCTTCTT TCTGAGCCAC TAACAGGATC CGCTCAACCT 
GCGACTCCAT CAGGTGTCCC AGCCGGGGCG GACGGTTCAG CGTTTACTCC TGTTCCTCTC 
AGTAAGCAAT CTAACTTGCC TCCAAAACAA AGAGCCGGTC AACGAAGACC TTCCATTGGC 
TTAAAGGCTC GCTGGCAGAA ATCTCGCCCC AACAGCATTG AGTGCTTAGT GGAAGGGGAC 
AACGATGATC CCAAAAGTAA ATGCAGTACC CTTCCACCTG CAATGGAGCA AATCCCTACT 
CTGAGATACA AACCTTTAGA ACATACTCAG AGAATAAACT TCACCCCGCA GCTTGAGCTG 
ACATCCAGGT TCTTCAGCAG TGCAGCTAAG CAGAAGGATG GAGAAAACAA GGGATCTTTA 
TTTTGGGCTC TCACAAGGGG ACGCTCTTCT GTTCGAAGAC CGTCAGGTCC CGCTAAACGA 
CTCAGTCTGG CTCATTCACT TAATGATCTT GAGTCGGCTG CATCAGATGG AGAGCGCAGT 
GGTGGTGGTG TCCAGCGTCA CGACTGCAGC AGCGTGAGCA GTTTGGACAG TAACGGTAGT 
CTGCCCAGCG TCACCAACCA CTGTCGTTTC ACAGAGCGCC GCGTTGCATT ATGGGCAGTC 
AGCTTCGAGC GATTGCTTCA GGATCCTGTG GGTGTCCGCT ATTTTTCTGA GTTTCTAAAG 
AAGGAGTTTA GCGAGGAAAA CATCTTATTC TGGCAGGCCT GTGAATGCTT CAGCCAAGTT 
CCAGAACATG ACAAAAAGCA GCTGTCCCAG AAGGCCAAAG AGATCTACAA CCGTTTCCTC 
TCAAGTAAAG CCACAATGCC AGTGAACATC GACAGTCAAG CTCAACTTGC GGACGATGTC 
CTGACAGCTC CACAGCCTAA CATGTTCAAA CAGCCACAGC TACAGATCTT CAACCTGATG 
AAGATGGACA GTTATGCGCG CTTCTTGAAG TCCACTCTCT ATCAGGAGTG TATGCTGGCA 
GAGGTGGAGG GTCGACCTCT GCCTGACCCC TGTCCCATCC CGTGCAGCCC TGCCCCATCC 
AAACACAGCT TCACCTCTGA CCGCTCCACA TTCTCCACTC CTAAAAAGGA AAACAGGAAG 
CCCAAAACAG GCAGGTCAAG CGATGATCTC AGAGAGAAAC ACTCTGATAA GAAAAACGGC 
TTCTTTTCCT GGTACAGATA TCCAAGTATT GGGAAAGGAC AAAAGAAAAG AGAGGCCCCA 
GATTTACCCT ACCACTGTAA TGGGCGGAGA GAGTCTCAAG GTTCTCTGTC TTCTGGAGCG 
AGTCAAGAGC TCAACACTTC CTGTTCAGGA GGAAAGAATG AGTGTGAGCT AAGAGGTTCG 
AGTTGTGCTT TGGATAAGGA GCGAGAGCGC TGCAGCAGGA CATGTACAGT AACACTGCCC 
GACGGCTCCA GCTGCTCTAT TCCTCTCCGA CAGGGAGCCT CCATCAGACA AGTGCTGCTA 
GAGCTCTGCC AAAAACAACA CATCAACCTG GCTGCTGTCG ACCTCTTTCT CACCGGTGGA 
GAGAAGCCTT TGGTCTTGGA TCAAGATAGT ATTACTCTCA GCTCCAGGGA ACTTCGTCTT 
GAGAAGCGCA CTTTGTTCAG ATTGGACCTG GTACCCATTA ACAGATCGGT TGGGTTGAAA 
GCGAAGCCCA CAAAACCTGT GACTGAGGTT CTACGGCCTG TGGTTGCCAA GTATGGACTC 
CAGCTTGGCG ATTTGGTGGC CAGAATAAGT GGCGAGACAG AGGTACTCGA TCTTGGAGTG 
CCCATATCAA ACCTTGATGG CCTGCGAGTT GTCTTGGAGA GGGCAGATCC AGCCAAAGCA 
GAAAAGTCTA AAACACTCAA CTCCAAAACA CCAGCTGCCA AGAAAGGGTT TCCAAGAGAG 
AGAGACGATA ACTCGACAGT GAAACCATTG AAAGCAGATG TTGAAGACAA GCATCCCCGC 
ACAGCTGCGT CTGAGAGGAA GAAAGGCAAA AAGAATCATA TAGATGAAAC TGAAGAGTTT 
TTCGAGTTGT TGAGTCGTGC TCAGAGCAGT CGTGTTGACG ACCAGAGAGG CCTGCTGAGA 
AAAGAAGATC TTGTCCTTCC CGACTTCCTT CGAGTAAACC CTGATCCCAA ACCTCAATCT 
CCTCCGTCCT GTTCAACCCC GACCTCTCAT AAACCCGGCC ACACCACTAC CACCTCCACA 
AACCCCCATC CCCCTAAACC CAGCCCCTGT AACGGACACC AAGCCTCAGC CCCGTTCAGC 
GCGCCACTCT CCCCCATCCT GCGTTCTGCT GGTGCCCGCG AGCAGGAGGA GGAGGAAGGC 
CTAGGAGATT TGACTCTGGT GGCGGAGGGT GACATCAGCA GCCCAAACTC CACCCTGCTC 
CCGCCTCCAC CGCCCGTATC ACCACCGCCT TATGATGGTA GTCTAGCAGA GGCCAACTTT 
ACTCCTCCAC CGGCTGTTCA CGGCACAGGT ACAGAAGAAG AAAACACGAG CACTTCAGCA 
GTAAACAGCT CAACAGCACA GCCGGACTTG CGTGTGCGGG ACAAGGGTGT CACTCTGGAG 
GACAGTTTTG AAGGATACGC AGCTGAACTT CGCCAGTGCC AGAACAAAAT GAGAAACGGC 
GTTTATCCCT CCACAGAAGC CCCCCGTCCC CTCAGCGGTG TGCTGGAAGT CAACGAGAGC 
GACACTGTGC AATACAAAGC AACGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301361.1
CDS	241..4353
Translation	MRLLDRPISPAVRRVEMERGRAGYGFTIAGQAPCVLRGIIKGSPAHSVGLLPGDRVLRVNDTDVSEASHDVVVKLIGTSSKSLCLLVTSGDRPPMACCSSDEELGCQNKSKRPWLNPGIKDPFPGKLDGRDLKEPILQSVGSFDTVFEISDQGTMTPHQEKQKKQRPLSEPDVSYWLQQQQRSKGNVDILSQDETSRVVSEDSVFSDLQSQNDLASDSSILNVAMIVGYINSMELELVMSQTEEDALQVIRQCISKIGIEQKTHSLVLMKVKCNCVQLCDDRDVVLASYPAENLALCVICTEDSRFFGLVSIDSTKNIDTGRQTSTLKTLCHVFFIDPELYEHKEHQSIIGHFGIPCTPDPDTQSCLEFPQTPHSISHFISVLYADMGDYIEKLRLKLDSENPDEGSQNARNSGSGSGSDSGIGNASPEERDSLIGQWSFTSPNICNPPPPYYSQHRNSTFLKPECRCLLSEPLTGSAQPATPSGVPAGADGSAFTPVPLSKQSNLPPKQRAGQRRPSIGLKARWQKSRPNSIECLVEGDNDDPKSKCSTLPPAMEQIPTLRYKPLEHTQRINFTPQLELTSRFFSSAAKQKDGENKGSLFWALTRGRSSVRRPSGPAKRLSLAHSLNDLESAASDGERSGGGVQRHDCSSVSSLDSNGSLPSVTNHCRFTERRVALWAVSFERLLQDPVGVRYFSEFLKKEFSEENILFWQACECFSQVPEHDKKQLSQKAKEIYNRFLSSKATMPVNIDSQAQLADDVLTAPQPNMFKQPQLQIFNLMKMDSYARFLKSTLYQECMLAEVEGRPLPDPCPIPCSPAPSKHSFTSDRSTFSTPKKENRKPKTGRSSDDLREKHSDKKNGFFSWYRYPSIGKGQKKREAPDLPYHCNGRRESQGSLSSGASQELNTSCSGGKNECELRGSSCALDKERERCSRTCTVTLPDGSSCSIPLRQGASIRQVLLELCQKQHINLAAVDLFLTGGEKPLVLDQDSITLSSRELRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLQLGDLVARISGETEVLDLGVPISNLDGLRVVLERADPAKAEKSKTLNSKTPAAKKGFPRERDDNSTVKPLKADVEDKHPRTAASERKKGKKNHIDETEEFFELLSRAQSSRVDDQRGLLRKEDLVLPDFLRVNPDPKPQSPPSCSTPTSHKPGHTTTTSTNPHPPKPSPCNGHQASAPFSAPLSPILRSAGAREQEEEEGLGDLTLVAEGDISSPNSTLLPPPPPVSPPPYDGSLAEANFTPPPAVHGTGTEEENTSTSAVNSSTAQPDLRVRDKGVTLEDSFEGYAAELRQCQNKMRNGVYPSTEAPRPLSGVLEVNESDTVQYKATFV

Target ORF information:

RefSeq Version	XM_009303086.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio regulator of G-protein signaling 12a (rgs12a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303086.2

ORF Insert Sequence:

ATGAGGCTTT TGGACCGTCC CATCAGCCCG GCGGTCCGCA GGGTGGAAAT GGAGCGTGGC 
AGAGCTGGCT ATGGATTCAC CATTGCCGGT CAAGCACCAT GTGTTCTCAG AGGAATAATA 
AAAGGGAGTC CGGCGCACAG TGTAGGACTA CTACCTGGGG ATCGTGTCTT GCGTGTAAAT 
GACACAGATG TGTCTGAAGC ATCACATGAT GTTGTGGTGA AGTTGATCGG TACTTCATCA 
AAGTCACTGT GTTTGCTGGT CACATCAGGT GATCGACCAC CCATGGCCTG CTGCTCCAGT 
GACGAGGAAC TGGGTTGTCA GAATAAGAGT AAACGTCCGT GGCTCAATCC TGGAATAAAG 
GACCCTTTCC CAGGTAAACT TGATGGCAGG GATTTGAAGG AACCAATTCT TCAGTCGGTT 
GGAAGTTTTG ATACCGTCTT TGAGATCTCA GATCAGGGCA CCATGACCCC ACATCAGGAG 
AAGCAGAAAA AACAAAGACC TTTGTCTGAA CCAGATGTGT CTTACTGGTT GCAGCAGCAG 
CAGAGATCCA AAGGGAATGT CGATATTCTT TCACAGGATG AAACATCTCG AGTAGTGAGT 
GAGGATTCTG TGTTTTCAGA CCTTCAGAGT CAGAATGACC TTGCATCTGA CTCCAGCATC 
CTGAACGTTG CAATGATTGT CGGATACATT AATTCCATGG AACTGGAGCT AGTAATGTCA 
CAAACTGAGG AGGATGCATT GCAGGTCATC CGGCAATGCA TTAGTAAGAT TGGTATTGAG 
CAGAAGACTC ATTCGCTGGT CTTAATGAAG GTTAAATGCA ACTGTGTTCA ACTTTGCGAT 
GACCGAGATG TTGTTCTTGC ATCTTACCCA GCAGAAAACC TGGCTCTTTG CGTAATTTGC 
ACAGAAGACA GCAGGTTCTT TGGCCTTGTC TCGATTGATT CAACCAAAAA TATAGACACC 
GGGAGGCAGA CAAGTACCTT GAAGACATTA TGTCATGTTT TTTTCATAGA CCCAGAGCTT 
TATGAACACA AGGAGCACCA AAGCATCATC GGACACTTTG GGATTCCCTG CACCCCAGAT 
CCAGACACTC AAAGCTGTTT GGAGTTTCCT CAAACCCCCC ACTCCATTTC ACACTTCATA 
TCCGTCCTCT ATGCAGACAT GGGAGATTAC ATTGAAAAAC TAAGACTTAA ACTTGACAGT 
GAGAATCCTG ACGAAGGATC CCAAAATGCA CGGAACAGCG GCAGTGGCAG CGGCAGTGAT 
AGCGGGATTG GAAATGCTTC ACCAGAGGAA AGAGACAGTC TCATTGGTCA GTGGAGCTTC 
ACATCTCCAA ACATTTGCAA TCCACCACCA CCATATTATT CCCAGCACAG GAATAGTACA 
TTCCTCAAAC CAGAGTGCAG ATGCCTTCTT TCTGAGCCAC TAACAGGATC CGCTCAACCT 
GCGACTCCAT CAGGTGTCCC AGCCGGGGCG GACGGTTCAG CGTTTACTCC TGTTCCTCTC 
AGTAAGCAAT CTAACTTGCC TCCAAAACAA AGAGCCGGTC AACGAAGACC TTCCATTGGC 
TTAAAGGCTC GCTGGCAGAA ATCTCGCCCC AACAGCATTG AGTGCTTAGT GGAAGGGGAC 
AACGATGATC CCAAAAGTAA ATGCAGTACC CTTCCACCTG CAATGGAGCA AATCCCTACT 
CTGAGATACA AACCTTTAGA ACATACTCAG AGAATAAACT TCACCCCGCA GCTTGAGCTG 
ACATCCAGGT TCTTCAGCAG TGCAGCTAAG CAGAAGGATG GAGAAAACAA GGGATCTTTA 
TTTTGGGCTC TCACAAGGGG ACGCTCTTCT GTTCGAAGAC CGTCAGGTCC CGCTAAACGA 
CTCAGTCTGG CTCATTCACT TAATGATCTT GAGTCGGCTG CATCAGATGG AGAGCGCAGT 
GGTGGTGGTG TCCAGCGTCA CGACTGCAGC AGCGTGAGCA GTTTGGACAG TAACGGTAGT 
CTGCCCAGCG TCACCAACCA CTGTCGTTTC ACAGAGCGCC GCGTTGCATT ATGGGCAGTC 
AGCTTCGAGC GATTGCTTCA GGATCCTGTG GGTGTCCGCT ATTTTTCTGA GTTTCTAAAG 
AAGGAGTTTA GCGAGGAAAA CATCTTATTC TGGCAGGCCT GTGAATGCTT CAGCCAAGTT 
CCAGAACATG ACAAAAAGCA GCTGTCCCAG AAGGCCAAAG AGATCTACAA CCGTTTCCTC 
TCAAGTAAAG CCACAATGCC AGTGAACATC GACAGTCAAG CTCAACTTGC GGACGATGTC 
CTGACAGCTC CACAGCCTAA CATGTTCAAA CAGCCACAGC TACAGATCTT CAACCTGATG 
AAGATGGACA GTTATGCGCG CTTCTTGAAG TCCACTCTCT ATCAGGAGTG TATGCTGGCA 
GAGGTGGAGG GTCGACCTCT GCCTGACCCC TGTCCCATCC CGTGCAGCCC TGCCCCATCC 
AAACACAGCT TCACCTCTGA CCGCTCCACA TTCTCCACTC CTAAAAAGGA AAACAGGAAG 
CCCAAAACAG GCAGGTCAAG CGATGATCTC AGAGAGAAAC ACTCTGATAA GAAAAACGGC 
TTCTTTTCCT GGTACAGATA TCCAAGTATT GGGAAAGGAC AAAAGAAAAG AGAGGCCCCA 
GATTTACCCT ACCACTGTAA TGGGCGGAGA GAGTCTCAAG GTTCTCTGTC TTCTGGAGCG 
AGTCAAGAGC TCAACACTTC CTGTTCAGGA GGAAAGAATG AGTGTGAGCT AAGAGGTTCG 
AGTTGTGCTT TGGATAAGGA GCGAGAGCGC TGCAGCAGGA CATGTACAGT AACACTGCCC 
GACGGCTCCA GCTGCTCTAT TCCTCTCCGA CAGGGAGCCT CCATCAGACA AGTGCTGCTA 
GAGCTCTGCC AAAAACAACA CATCAACCTG GCTGCTGTCG ACCTCTTTCT CACCGGTGGA 
GAGAAGCCTT TGGTCTTGGA TCAAGATAGT ATTACTCTCA GCTCCAGGGA ACTTCGTCTT 
GAGAAGCGCA CTTTGTTCAG ATTGGACCTG GTACCCATTA ACAGATCGGT TGGGTTGAAA 
GCGAAGCCCA CAAAACCTGT GACTGAGGTT CTACGGCCTG TGGTTGCCAA GTATGGACTC 
CAGCTTGGCG ATTTGGTGGC CAGAATAAGT GGCGAGACAG AGGTACTCGA TCTTGGAGTG 
CCCATATCAA ACCTTGATGG CCTGCGAGTT GTCTTGGAGA GGGCAGATCC AGCCAAAGCA 
GAAAAGTCTA AAACACTCAA CTCCAAAACA CCAGCTGCCA AGAAAGGGTT TCCAAGAGAG 
AGAGACGATA ACTCGACAGT GAAACCATTG AAAGCAGATG TTGAAGACAA GCATCCCCGC 
ACAGCTGCGT CTGAGAGGAA GAAAGGCAAA AAGAATCATA TAGATGAAAC TGAAGAGTTT 
TTCGAGTTGT TGAGTCGTGC TCAGAGCAGT CGTGTTGACG ACCAGAGAGG CCTGCTGAGA 
AAAGAAGATC TTGTCCTTCC CGACTTCCTT CGAGTAAACC CTGATCCCAA ACCTCAATCT 
CCTCCGTCCT GTTCAACCCC GACCTCTCAT AAACCCGGCC ACACCACTAC CACCTCCACA 
AACCCCCATC CCCCTAAACC CAGCCCCTGT AACGGACACC AAGCCTCAGC CCCGTTCAGC 
GCGCCACTCT CCCCCATCCT GCGTTCTGCT GGTGCCCGCG AGCAGGAGGA GGAGGAAGGC 
CTAGGAGATT TGACTCTGGT GGCGGAGGGT GACATCAGCA GCCCAAACTC CACCCTGCTC 
CCGCCTCCAC CGCCCGTATC ACCACCGCCT TATGATGGTA GTCTAGCAGA GGCCAACTTT 
ACTCCTCCAC CGGCTGTTCA CGGCACAGGT ACAGAAGAAG AAAACACGAG CACTTCAGCA 
GTAAACAGCT CAACAGCACA GCCGGACTTG CGTGTGCGGG ACAAGGGTGT CACTCTGGAG 
GACAGTTTTG AAGGATACGC AGCTGAACTT CGCCAGTGCC AGAACAAAAT GAGAAACGGC 
GTTTATCCCT CCACAGAAGC CCCCCGTCCC CTCAGCGGTG TGCTGGAAGT CAACGAGAGC 
GACACTGTGC AATACAAAGC AACGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rgs12a ( XM_009303086.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301361.1 Danio rerio regulator of G protein signaling 12a (rgs12a), transcript variant X3, mRNA.

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CloneID	ODa45980
Clone ID Related Accession (Same CDS sequence)	XM_009303086.2 , XM_009303086.3
Accession Version	XM_009303086.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	regulator of G-protein signaling 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303086.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTTT TGGACCGTCC CATCAGCCCG GCGGTCCGCA GGGTGGAAAT GGAGCGTGGC 
AGAGCTGGCT ATGGATTCAC CATTGCCGGT CAAGCACCAT GTGTTCTCAG AGGAATAATA 
AAAGGGAGTC CGGCGCACAG TGTAGGACTA CTACCTGGGG ATCGTGTCTT GCGTGTAAAT 
GACACAGATG TGTCTGAAGC ATCACATGAT GTTGTGGTGA AGTTGATCGG TACTTCATCA 
AAGTCACTGT GTTTGCTGGT CACATCAGGT GATCGACCAC CCATGGCCTG CTGCTCCAGT 
GACGAGGAAC TGGGTTGTCA GAATAAGAGT AAACGTCCGT GGCTCAATCC TGGAATAAAG 
GACCCTTTCC CAGGTAAACT TGATGGCAGG GATTTGAAGG AACCAATTCT TCAGTCGGTT 
GGAAGTTTTG ATACCGTCTT TGAGATCTCA GATCAGGGCA CCATGACCCC ACATCAGGAG 
AAGCAGAAAA AACAAAGACC TTTGTCTGAA CCAGATGTGT CTTACTGGTT GCAGCAGCAG 
CAGAGATCCA AAGGGAATGT CGATATTCTT TCACAGGATG AAACATCTCG AGTAGTGAGT 
GAGGATTCTG TGTTTTCAGA CCTTCAGAGT CAGAATGACC TTGCATCTGA CTCCAGCATC 
CTGAACGTTG CAATGATTGT CGGATACATT AATTCCATGG AACTGGAGCT AGTAATGTCA 
CAAACTGAGG AGGATGCATT GCAGGTCATC CGGCAATGCA TTAGTAAGAT TGGTATTGAG 
CAGAAGACTC ATTCGCTGGT CTTAATGAAG GTTAAATGCA ACTGTGTTCA ACTTTGCGAT 
GACCGAGATG TTGTTCTTGC ATCTTACCCA GCAGAAAACC TGGCTCTTTG CGTAATTTGC 
ACAGAAGACA GCAGGTTCTT TGGCCTTGTC TCGATTGATT CAACCAAAAA TATAGACACC 
GGGAGGCAGA CAAGTACCTT GAAGACATTA TGTCATGTTT TTTTCATAGA CCCAGAGCTT 
TATGAACACA AGGAGCACCA AAGCATCATC GGACACTTTG GGATTCCCTG CACCCCAGAT 
CCAGACACTC AAAGCTGTTT GGAGTTTCCT CAAACCCCCC ACTCCATTTC ACACTTCATA 
TCCGTCCTCT ATGCAGACAT GGGAGATTAC ATTGAAAAAC TAAGACTTAA ACTTGACAGT 
GAGAATCCTG ACGAAGGATC CCAAAATGCA CGGAACAGCG GCAGTGGCAG CGGCAGTGAT 
AGCGGGATTG GAAATGCTTC ACCAGAGGAA AGAGACAGTC TCATTGGTCA GTGGAGCTTC 
ACATCTCCAA ACATTTGCAA TCCACCACCA CCATATTATT CCCAGCACAG GAATAGTACA 
TTCCTCAAAC CAGAGTGCAG ATGCCTTCTT TCTGAGCCAC TAACAGGATC CGCTCAACCT 
GCGACTCCAT CAGGTGTCCC AGCCGGGGCG GACGGTTCAG CGTTTACTCC TGTTCCTCTC 
AGTAAGCAAT CTAACTTGCC TCCAAAACAA AGAGCCGGTC AACGAAGACC TTCCATTGGC 
TTAAAGGCTC GCTGGCAGAA ATCTCGCCCC AACAGCATTG AGTGCTTAGT GGAAGGGGAC 
AACGATGATC CCAAAAGTAA ATGCAGTACC CTTCCACCTG CAATGGAGCA AATCCCTACT 
CTGAGATACA AACCTTTAGA ACATACTCAG AGAATAAACT TCACCCCGCA GCTTGAGCTG 
ACATCCAGGT TCTTCAGCAG TGCAGCTAAG CAGAAGGATG GAGAAAACAA GGGATCTTTA 
TTTTGGGCTC TCACAAGGGG ACGCTCTTCT GTTCGAAGAC CGTCAGGTCC CGCTAAACGA 
CTCAGTCTGG CTCATTCACT TAATGATCTT GAGTCGGCTG CATCAGATGG AGAGCGCAGT 
GGTGGTGGTG TCCAGCGTCA CGACTGCAGC AGCGTGAGCA GTTTGGACAG TAACGGTAGT 
CTGCCCAGCG TCACCAACCA CTGTCGTTTC ACAGAGCGCC GCGTTGCATT ATGGGCAGTC 
AGCTTCGAGC GATTGCTTCA GGATCCTGTG GGTGTCCGCT ATTTTTCTGA GTTTCTAAAG 
AAGGAGTTTA GCGAGGAAAA CATCTTATTC TGGCAGGCCT GTGAATGCTT CAGCCAAGTT 
CCAGAACATG ACAAAAAGCA GCTGTCCCAG AAGGCCAAAG AGATCTACAA CCGTTTCCTC 
TCAAGTAAAG CCACAATGCC AGTGAACATC GACAGTCAAG CTCAACTTGC GGACGATGTC 
CTGACAGCTC CACAGCCTAA CATGTTCAAA CAGCCACAGC TACAGATCTT CAACCTGATG 
AAGATGGACA GTTATGCGCG CTTCTTGAAG TCCACTCTCT ATCAGGAGTG TATGCTGGCA 
GAGGTGGAGG GTCGACCTCT GCCTGACCCC TGTCCCATCC CGTGCAGCCC TGCCCCATCC 
AAACACAGCT TCACCTCTGA CCGCTCCACA TTCTCCACTC CTAAAAAGGA AAACAGGAAG 
CCCAAAACAG GCAGGTCAAG CGATGATCTC AGAGAGAAAC ACTCTGATAA GAAAAACGGC 
TTCTTTTCCT GGTACAGATA TCCAAGTATT GGGAAAGGAC AAAAGAAAAG AGAGGCCCCA 
GATTTACCCT ACCACTGTAA TGGGCGGAGA GAGTCTCAAG GTTCTCTGTC TTCTGGAGCG 
AGTCAAGAGC TCAACACTTC CTGTTCAGGA GGAAAGAATG AGTGTGAGCT AAGAGGTTCG 
AGTTGTGCTT TGGATAAGGA GCGAGAGCGC TGCAGCAGGA CATGTACAGT AACACTGCCC 
GACGGCTCCA GCTGCTCTAT TCCTCTCCGA CAGGGAGCCT CCATCAGACA AGTGCTGCTA 
GAGCTCTGCC AAAAACAACA CATCAACCTG GCTGCTGTCG ACCTCTTTCT CACCGGTGGA 
GAGAAGCCTT TGGTCTTGGA TCAAGATAGT ATTACTCTCA GCTCCAGGGA ACTTCGTCTT 
GAGAAGCGCA CTTTGTTCAG ATTGGACCTG GTACCCATTA ACAGATCGGT TGGGTTGAAA 
GCGAAGCCCA CAAAACCTGT GACTGAGGTT CTACGGCCTG TGGTTGCCAA GTATGGACTC 
CAGCTTGGCG ATTTGGTGGC CAGAATAAGT GGCGAGACAG AGGTACTCGA TCTTGGAGTG 
CCCATATCAA ACCTTGATGG CCTGCGAGTT GTCTTGGAGA GGGCAGATCC AGCCAAAGCA 
GAAAAGTCTA AAACACTCAA CTCCAAAACA CCAGCTGCCA AGAAAGGGTT TCCAAGAGAG 
AGAGACGATA ACTCGACAGT GAAACCATTG AAAGCAGATG TTGAAGACAA GCATCCCCGC 
ACAGCTGCGT CTGAGAGGAA GAAAGGCAAA AAGAATCATA TAGATGAAAC TGAAGAGTTT 
TTCGAGTTGT TGAGTCGTGC TCAGAGCAGT CGTGTTGACG ACCAGAGAGG CCTGCTGAGA 
AAAGAAGATC TTGTCCTTCC CGACTTCCTT CGAGTAAACC CTGATCCCAA ACCTCAATCT 
CCTCCGTCCT GTTCAACCCC GACCTCTCAT AAACCCGGCC ACACCACTAC CACCTCCACA 
AACCCCCATC CCCCTAAACC CAGCCCCTGT AACGGACACC AAGCCTCAGC CCCGTTCAGC 
GCGCCACTCT CCCCCATCCT GCGTTCTGCT GGTGCCCGCG AGCAGGAGGA GGAGGAAGGC 
CTAGGAGATT TGACTCTGGT GGCGGAGGGT GACATCAGCA GCCCAAACTC CACCCTGCTC 
CCGCCTCCAC CGCCCGTATC ACCACCGCCT TATGATGGTA GTCTAGCAGA GGCCAACTTT 
ACTCCTCCAC CGGCTGTTCA CGGCACAGGT ACAGAAGAAG AAAACACGAG CACTTCAGCA 
GTAAACAGCT CAACAGCACA GCCGGACTTG CGTGTGCGGG ACAAGGGTGT CACTCTGGAG 
GACAGTTTTG AAGGATACGC AGCTGAACTT CGCCAGTGCC AGAACAAAAT GAGAAACGGC 
GTTTATCCCT CCACAGAAGC CCCCCGTCCC CTCAGCGGTG TGCTGGAAGT CAACGAGAGC 
GACACTGTGC AATACAAAGC AACGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301361.1
CDS	265..4377
Translation	MRLLDRPISPAVRRVEMERGRAGYGFTIAGQAPCVLRGIIKGSPAHSVGLLPGDRVLRVNDTDVSEASHDVVVKLIGTSSKSLCLLVTSGDRPPMACCSSDEELGCQNKSKRPWLNPGIKDPFPGKLDGRDLKEPILQSVGSFDTVFEISDQGTMTPHQEKQKKQRPLSEPDVSYWLQQQQRSKGNVDILSQDETSRVVSEDSVFSDLQSQNDLASDSSILNVAMIVGYINSMELELVMSQTEEDALQVIRQCISKIGIEQKTHSLVLMKVKCNCVQLCDDRDVVLASYPAENLALCVICTEDSRFFGLVSIDSTKNIDTGRQTSTLKTLCHVFFIDPELYEHKEHQSIIGHFGIPCTPDPDTQSCLEFPQTPHSISHFISVLYADMGDYIEKLRLKLDSENPDEGSQNARNSGSGSGSDSGIGNASPEERDSLIGQWSFTSPNICNPPPPYYSQHRNSTFLKPECRCLLSEPLTGSAQPATPSGVPAGADGSAFTPVPLSKQSNLPPKQRAGQRRPSIGLKARWQKSRPNSIECLVEGDNDDPKSKCSTLPPAMEQIPTLRYKPLEHTQRINFTPQLELTSRFFSSAAKQKDGENKGSLFWALTRGRSSVRRPSGPAKRLSLAHSLNDLESAASDGERSGGGVQRHDCSSVSSLDSNGSLPSVTNHCRFTERRVALWAVSFERLLQDPVGVRYFSEFLKKEFSEENILFWQACECFSQVPEHDKKQLSQKAKEIYNRFLSSKATMPVNIDSQAQLADDVLTAPQPNMFKQPQLQIFNLMKMDSYARFLKSTLYQECMLAEVEGRPLPDPCPIPCSPAPSKHSFTSDRSTFSTPKKENRKPKTGRSSDDLREKHSDKKNGFFSWYRYPSIGKGQKKREAPDLPYHCNGRRESQGSLSSGASQELNTSCSGGKNECELRGSSCALDKERERCSRTCTVTLPDGSSCSIPLRQGASIRQVLLELCQKQHINLAAVDLFLTGGEKPLVLDQDSITLSSRELRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLQLGDLVARISGETEVLDLGVPISNLDGLRVVLERADPAKAEKSKTLNSKTPAAKKGFPRERDDNSTVKPLKADVEDKHPRTAASERKKGKKNHIDETEEFFELLSRAQSSRVDDQRGLLRKEDLVLPDFLRVNPDPKPQSPPSCSTPTSHKPGHTTTTSTNPHPPKPSPCNGHQASAPFSAPLSPILRSAGAREQEEEEGLGDLTLVAEGDISSPNSTLLPPPPPVSPPPYDGSLAEANFTPPPAVHGTGTEEENTSTSAVNSSTAQPDLRVRDKGVTLEDSFEGYAAELRQCQNKMRNGVYPSTEAPRPLSGVLEVNESDTVQYKATFV

Target ORF information:

RefSeq Version	XM_009303086.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio regulator of G protein signaling 12a (rgs12a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303086.3

ORF Insert Sequence:

ATGAGGCTTT TGGACCGTCC CATCAGCCCG GCGGTCCGCA GGGTGGAAAT GGAGCGTGGC 
AGAGCTGGCT ATGGATTCAC CATTGCCGGT CAAGCACCAT GTGTTCTCAG AGGAATAATA 
AAAGGGAGTC CGGCGCACAG TGTAGGACTA CTACCTGGGG ATCGTGTCTT GCGTGTAAAT 
GACACAGATG TGTCTGAAGC ATCACATGAT GTTGTGGTGA AGTTGATCGG TACTTCATCA 
AAGTCACTGT GTTTGCTGGT CACATCAGGT GATCGACCAC CCATGGCCTG CTGCTCCAGT 
GACGAGGAAC TGGGTTGTCA GAATAAGAGT AAACGTCCGT GGCTCAATCC TGGAATAAAG 
GACCCTTTCC CAGGTAAACT TGATGGCAGG GATTTGAAGG AACCAATTCT TCAGTCGGTT 
GGAAGTTTTG ATACCGTCTT TGAGATCTCA GATCAGGGCA CCATGACCCC ACATCAGGAG 
AAGCAGAAAA AACAAAGACC TTTGTCTGAA CCAGATGTGT CTTACTGGTT GCAGCAGCAG 
CAGAGATCCA AAGGGAATGT CGATATTCTT TCACAGGATG AAACATCTCG AGTAGTGAGT 
GAGGATTCTG TGTTTTCAGA CCTTCAGAGT CAGAATGACC TTGCATCTGA CTCCAGCATC 
CTGAACGTTG CAATGATTGT CGGATACATT AATTCCATGG AACTGGAGCT AGTAATGTCA 
CAAACTGAGG AGGATGCATT GCAGGTCATC CGGCAATGCA TTAGTAAGAT TGGTATTGAG 
CAGAAGACTC ATTCGCTGGT CTTAATGAAG GTTAAATGCA ACTGTGTTCA ACTTTGCGAT 
GACCGAGATG TTGTTCTTGC ATCTTACCCA GCAGAAAACC TGGCTCTTTG CGTAATTTGC 
ACAGAAGACA GCAGGTTCTT TGGCCTTGTC TCGATTGATT CAACCAAAAA TATAGACACC 
GGGAGGCAGA CAAGTACCTT GAAGACATTA TGTCATGTTT TTTTCATAGA CCCAGAGCTT 
TATGAACACA AGGAGCACCA AAGCATCATC GGACACTTTG GGATTCCCTG CACCCCAGAT 
CCAGACACTC AAAGCTGTTT GGAGTTTCCT CAAACCCCCC ACTCCATTTC ACACTTCATA 
TCCGTCCTCT ATGCAGACAT GGGAGATTAC ATTGAAAAAC TAAGACTTAA ACTTGACAGT 
GAGAATCCTG ACGAAGGATC CCAAAATGCA CGGAACAGCG GCAGTGGCAG CGGCAGTGAT 
AGCGGGATTG GAAATGCTTC ACCAGAGGAA AGAGACAGTC TCATTGGTCA GTGGAGCTTC 
ACATCTCCAA ACATTTGCAA TCCACCACCA CCATATTATT CCCAGCACAG GAATAGTACA 
TTCCTCAAAC CAGAGTGCAG ATGCCTTCTT TCTGAGCCAC TAACAGGATC CGCTCAACCT 
GCGACTCCAT CAGGTGTCCC AGCCGGGGCG GACGGTTCAG CGTTTACTCC TGTTCCTCTC 
AGTAAGCAAT CTAACTTGCC TCCAAAACAA AGAGCCGGTC AACGAAGACC TTCCATTGGC 
TTAAAGGCTC GCTGGCAGAA ATCTCGCCCC AACAGCATTG AGTGCTTAGT GGAAGGGGAC 
AACGATGATC CCAAAAGTAA ATGCAGTACC CTTCCACCTG CAATGGAGCA AATCCCTACT 
CTGAGATACA AACCTTTAGA ACATACTCAG AGAATAAACT TCACCCCGCA GCTTGAGCTG 
ACATCCAGGT TCTTCAGCAG TGCAGCTAAG CAGAAGGATG GAGAAAACAA GGGATCTTTA 
TTTTGGGCTC TCACAAGGGG ACGCTCTTCT GTTCGAAGAC CGTCAGGTCC CGCTAAACGA 
CTCAGTCTGG CTCATTCACT TAATGATCTT GAGTCGGCTG CATCAGATGG AGAGCGCAGT 
GGTGGTGGTG TCCAGCGTCA CGACTGCAGC AGCGTGAGCA GTTTGGACAG TAACGGTAGT 
CTGCCCAGCG TCACCAACCA CTGTCGTTTC ACAGAGCGCC GCGTTGCATT ATGGGCAGTC 
AGCTTCGAGC GATTGCTTCA GGATCCTGTG GGTGTCCGCT ATTTTTCTGA GTTTCTAAAG 
AAGGAGTTTA GCGAGGAAAA CATCTTATTC TGGCAGGCCT GTGAATGCTT CAGCCAAGTT 
CCAGAACATG ACAAAAAGCA GCTGTCCCAG AAGGCCAAAG AGATCTACAA CCGTTTCCTC 
TCAAGTAAAG CCACAATGCC AGTGAACATC GACAGTCAAG CTCAACTTGC GGACGATGTC 
CTGACAGCTC CACAGCCTAA CATGTTCAAA CAGCCACAGC TACAGATCTT CAACCTGATG 
AAGATGGACA GTTATGCGCG CTTCTTGAAG TCCACTCTCT ATCAGGAGTG TATGCTGGCA 
GAGGTGGAGG GTCGACCTCT GCCTGACCCC TGTCCCATCC CGTGCAGCCC TGCCCCATCC 
AAACACAGCT TCACCTCTGA CCGCTCCACA TTCTCCACTC CTAAAAAGGA AAACAGGAAG 
CCCAAAACAG GCAGGTCAAG CGATGATCTC AGAGAGAAAC ACTCTGATAA GAAAAACGGC 
TTCTTTTCCT GGTACAGATA TCCAAGTATT GGGAAAGGAC AAAAGAAAAG AGAGGCCCCA 
GATTTACCCT ACCACTGTAA TGGGCGGAGA GAGTCTCAAG GTTCTCTGTC TTCTGGAGCG 
AGTCAAGAGC TCAACACTTC CTGTTCAGGA GGAAAGAATG AGTGTGAGCT AAGAGGTTCG 
AGTTGTGCTT TGGATAAGGA GCGAGAGCGC TGCAGCAGGA CATGTACAGT AACACTGCCC 
GACGGCTCCA GCTGCTCTAT TCCTCTCCGA CAGGGAGCCT CCATCAGACA AGTGCTGCTA 
GAGCTCTGCC AAAAACAACA CATCAACCTG GCTGCTGTCG ACCTCTTTCT CACCGGTGGA 
GAGAAGCCTT TGGTCTTGGA TCAAGATAGT ATTACTCTCA GCTCCAGGGA ACTTCGTCTT 
GAGAAGCGCA CTTTGTTCAG ATTGGACCTG GTACCCATTA ACAGATCGGT TGGGTTGAAA 
GCGAAGCCCA CAAAACCTGT GACTGAGGTT CTACGGCCTG TGGTTGCCAA GTATGGACTC 
CAGCTTGGCG ATTTGGTGGC CAGAATAAGT GGCGAGACAG AGGTACTCGA TCTTGGAGTG 
CCCATATCAA ACCTTGATGG CCTGCGAGTT GTCTTGGAGA GGGCAGATCC AGCCAAAGCA 
GAAAAGTCTA AAACACTCAA CTCCAAAACA CCAGCTGCCA AGAAAGGGTT TCCAAGAGAG 
AGAGACGATA ACTCGACAGT GAAACCATTG AAAGCAGATG TTGAAGACAA GCATCCCCGC 
ACAGCTGCGT CTGAGAGGAA GAAAGGCAAA AAGAATCATA TAGATGAAAC TGAAGAGTTT 
TTCGAGTTGT TGAGTCGTGC TCAGAGCAGT CGTGTTGACG ACCAGAGAGG CCTGCTGAGA 
AAAGAAGATC TTGTCCTTCC CGACTTCCTT CGAGTAAACC CTGATCCCAA ACCTCAATCT 
CCTCCGTCCT GTTCAACCCC GACCTCTCAT AAACCCGGCC ACACCACTAC CACCTCCACA 
AACCCCCATC CCCCTAAACC CAGCCCCTGT AACGGACACC AAGCCTCAGC CCCGTTCAGC 
GCGCCACTCT CCCCCATCCT GCGTTCTGCT GGTGCCCGCG AGCAGGAGGA GGAGGAAGGC 
CTAGGAGATT TGACTCTGGT GGCGGAGGGT GACATCAGCA GCCCAAACTC CACCCTGCTC 
CCGCCTCCAC CGCCCGTATC ACCACCGCCT TATGATGGTA GTCTAGCAGA GGCCAACTTT 
ACTCCTCCAC CGGCTGTTCA CGGCACAGGT ACAGAAGAAG AAAACACGAG CACTTCAGCA 
GTAAACAGCT CAACAGCACA GCCGGACTTG CGTGTGCGGG ACAAGGGTGT CACTCTGGAG 
GACAGTTTTG AAGGATACGC AGCTGAACTT CGCCAGTGCC AGAACAAAAT GAGAAACGGC 
GTTTATCCCT CCACAGAAGC CCCCCGTCCC CTCAGCGGTG TGCTGGAAGT CAACGAGAGC 
GACACTGTGC AATACAAAGC AACGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	rgs12a
Entrez Gene ID	570516
Full Name	regulator of G protein signaling 12a
Synonyms	fc13d03,wu:fc13d03
General protein information	Preferred Names regulator of G protein signaling 12a Names regulator of G-protein signaling 12 uncharacterized protein LOC570516
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

rgs12a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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