npnt cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	npnt
Entrez Gene ID	563273
Full Name	nephronectin
Synonyms	POEM,si:ch211-202n8.2
General protein information	Preferred Names nephronectin Names nephronectin nephronectin variant 1 nephronectin variant 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009294216.2	XP_009292491.1	nephronectin isoform X1
XM_005160098.4	XP_005160155.1	nephronectin isoform X2
XM_005160098.3	XP_005160155.1	nephronectin isoform X2
XM_009294219.3	XP_009292494.1	nephronectin isoform X3
XM_009294219.2	XP_009292494.1	nephronectin isoform X3
NM_001145580.1	NP_001139052.1	nephronectin precursor
XM_009294216.3	XP_009292491.1	nephronectin isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NPNT	XP_420498.3
Pan troglodytes (chimpanzee)	NPNT	XP_517382.3
	NPNT	XP_001084904.2
Bos taurus (cattle)	NPNT	XP_005207735.1
Homo sapiens (human)	NPNT	NP_001171619.1
Canis lupus familiaris (dog)	NPNT	XP_005639300.1
Danio rerio (zebrafish)	npnt	NP_001139052.1
Xenopus tropicalis (tropical clawed frog)	npnt	XP_004911145.1
Mus musculus (house mouse)	Npnt	NP_001274030.1

Gene Ontology
Bibliography

Related articles in PubMed

Podocytes regulate the glomerular basement membrane protein nephronectin by means of?miR-378a-3p in glomerular diseases.
M?ller-Deile J, Dannenberg J, Schroder P, Lin MH, Miner JH, Chen R, Br?sen JH, Thum T, Nystr?m J, Staggs LB, Haller H, Fiedler J, Lorenzen JM, Schiffer M
Kidney international92(4)836-849(2017 Oct)

Pharyngeal morphogenesis requires fras1-itga8-dependent epithelial-mesenchymal interaction.
Talbot JC, Nichols JT, Yan YL, Leonard IF, BreMiller RA, Amacher SL, Postlethwait JH, Kimmel CB
Developmental biology416(1)136-148(2016 08)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Gene responses in the central nervous system of zebrafish embryos exposed to the neurotoxicant methyl mercury.
Ho NY, Yang L, Legradi J, Armant O, Takamiya M, Rastegar S, Str?hle U
Environmental science & technology47(7)3316-25(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Morpholino-mediated knockdown of Npnt prevents proper heart valve leaflet formation and trabeculation and results in greater than 85% lethality at 7 days post-fertilization.
Title: Nephronectin regulates atrioventricular canal differentiation via Bmp4-Has2 signaling in zebrafish.

The following npnt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the npnt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31792	NM_001145580.1 Latest version!	Danio rerio nephronectin a (npnta), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa06176	XM_005160098.4 Latest version!	Danio rerio nephronectin (npnt), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa44023	XM_009294216.3 Latest version!	Danio rerio nephronectin (npnt), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa44024	XM_009294219.3 Latest version!	Danio rerio nephronectin (npnt), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa06176	XM_005160098.3	Danio rerio nephronectin (npnt), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa44024	XM_009294219.2	Danio rerio nephronectin (npnt), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa44023	XM_009294216.2	Danio rerio nephronectin (npnt), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

npnt ( NM_001145580.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001139052.1 Danio rerio nephronectin a (npnta), mRNA.

Price & Availability↑

CloneID	ODa31792
Clone ID Related Accession (Same CDS sequence)	NM_001145580.1
Accession Version	NM_001145580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	nephronectin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX537118.12. On Mar 5, 2009 this sequence version replaced XM_686637.3. ##Evidence-Data-START## Transcript exon combination :: GFIL01028484.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168448, SAMEA4476739 [ECO:0000348] ##Evidence-Data-END##

ATGTGGATCA TAAAGTTCAT GTTGATGTGG ACCTGCTGGA TCGCAGTGGA CGCGGACTTC 
GATGGCAGGT GGTCCCGACA GATGTCCTCA TCCAATGGTC TGTGTCGGTA CGGCGGCCGC 
ATTGACTGCT GCTGGGGCTG GACGAGAGTC TCCTGGGGCC AGTGCCAGCC CCTCTACGTC 
TTAACCCGCA GAGTAATCGG GATAAGGTGT CAGCCCCAAG CGCTATGTCA GCACGGATGC 
AAGCACGGAG AATGCGTGGG ACCCAACAAA TGCAAGTGCC ATCCAGGTTA TACAGGAAAA 
ACATGCAACC AAGACCTGAA CGAGTGTGGC CTGAAGCCTC GTCCTTGTAA GCACCGCTGC 
ATGAACACAT TCGGGAGCTT CAAGTGTTAC TGTCTGAATG GCTTCATGCT GCTGCCGGAT 
GGGAGCTGCG CCAATGCCCG AACTTGCAGC ATGGCGAACT GCCAGTATGG CTGTGAGGTA 
ATGAAAGGAG AGGTTCGCTG CCAGTGCCCG TCTCCAGGTC TACAGCTGGC TCCAGATGGC 
AGGACCTGTG TGGATGTGGA TGAATGTGCC GCAGGTCTTG CTGTTTGTCC CCGTTTCCGG 
AAGTGCATCA ACACATTTGG GAGTTACATC TGCAAGTGCC ATGACGGCTT TGACCTTCAG 
TACGTCAATG GGAAATATCA GTGTACAGAT GTCAATGAAT GCTCTTTGGG TCAGCACCAG 
TGTGGTCCAT ACGCCACCTG TTATAACACA CCTGGTTCAT ACAAGTGCAA GTGTAAAGAA 
GACTACAGAG GCGTGGGCTA CGACTGCAAA CCCATCCCAA AGGTGGTAAT TGACCCTCCA 
CGACCTGGAA AGACCACACC GAGCAGCAAT AACAACAAAG GCGGCAACAA AATTCCAGGA 
TCAGACCAAA AGAGGACCAC CACAACAGTA AGACCTCCAG TAACGGCCAA ACGCATCTCA 
CCTACAATCA CCACCACCAC CACTACCACC AAACCTCCTC CAACCAAAAA AATCACCCCT 
CCAGCAAGAG TCCCGGTGAC CACCACCCGT AAGCCATTCA TCCCAACAAG GAAGCCCCCT 
GTGCTGGTGA CAACCAAACC TAAGGTCCCG ACTCACCAGC ACACCACCAC AAAAGTCCCA 
GTGGTTACAG CAGTGGTTCC TTTTATCCCA ACACGCAGAC CTTTCACCAT CCCGTTTGTG 
ACACCAATTG ATAACAGCAT CAAAGACATC ACCCAGAAAC AAAGAGGCGA TGTTCACATA 
CCACGAAATC ATGGGAAGAA CAATGTTCTT GGTATCGATT TAGACATTGA ACTGGGCAAC 
ACCGAAGAGG AGCTGAAGGA TGACCCAGAG TCTGGCTATC TGAGCTGCTC ATTTGATCAC 
GGTTTGTGTG GATGGATCCA GCGCAGAGAG GGAGACCTTC ATTGGGAGAC ATCGGAAGAT 
CCTTCGGGTG GGAGGTACCT AACCATCTCT GAGGGCGGGG AGAAGCGTGG TGGGCGTGGT 
GCTCAGCTGA TCCTCCCCCT GAAAACCCCC TGGAACGAGG GGAACCTGTG CTTGGCCTTC 
AGACACAACA TGGCAGGTCA CCATGTGGGA ATGCTGCAGG TGTTCGTACA GAAAGGTCGG 
CAGCACAGTC CCGCTGTCTG GGGACGAACA GGAGGAAACG GCTGGAGGTC CACACAGATC 
ACCCTTTGGG GCAATGGGCT AGAAAGTGTG ATCGTGAAGG GCGAGCGGCG GAGAGGCCGC 
AAGGGCGAAA TTGCTCTGGA TGACATGAGT CTCAAACGAG GTTCCTGTCA GGAAGAGCAC 
AATCTGAGGA GACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139052.1
CDS	201..2018
Translation	MWIIKFMLMWTCWIAVDADFDGRWSRQMSSSNGLCRYGGRIDCCWGWTRVSWGQCQPLYVLTRRVIGIRCQPQALCQHGCKHGECVGPNKCKCHPGYTGKTCNQDLNECGLKPRPCKHRCMNTFGSFKCYCLNGFMLLPDGSCANARTCSMANCQYGCEVMKGEVRCQCPSPGLQLAPDGRTCVDVDECAAGLAVCPRFRKCINTFGSYICKCHDGFDLQYVNGKYQCTDVNECSLGQHQCGPYATCYNTPGSYKCKCKEDYRGVGYDCKPIPKVVIDPPRPGKTTPSSNNNKGGNKIPGSDQKRTTTTVRPPVTAKRISPTITTTTTTTKPPPTKKITPPARVPVTTTRKPFIPTRKPPVLVTTKPKVPTHQHTTTKVPVVTAVVPFIPTRRPFTIPFVTPIDNSIKDITQKQRGDVHIPRNHGKNNVLGIDLDIELGNTEEELKDDPESGYLSCSFDHGLCGWIQRREGDLHWETSEDPSGGRYLTISEGGEKRGGRGAQLILPLKTPWNEGNLCLAFRHNMAGHHVGMLQVFVQKGRQHSPAVWGRTGGNGWRSTQITLWGNGLESVIVKGERRRGRKGEIALDDMSLKRGSCQEEHNLRRL

Target ORF information:

RefSeq Version	NM_001145580.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nephronectin a (npnta), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145580.1

ORF Insert Sequence:

ATGTGGATCA TAAAGTTCAT GTTGATGTGG ACCTGCTGGA TCGCAGTGGA CGCGGACTTC 
GATGGCAGGT GGTCCCGACA GATGTCCTCA TCCAATGGTC TGTGTCGGTA CGGCGGCCGC 
ATTGACTGCT GCTGGGGCTG GACGAGAGTC TCCTGGGGCC AGTGCCAGCC CCTCTACGTC 
TTAACCCGCA GAGTAATCGG GATAAGGTGT CAGCCCCAAG CGCTATGTCA GCACGGATGC 
AAGCACGGAG AATGCGTGGG ACCCAACAAA TGCAAGTGCC ATCCAGGTTA TACAGGAAAA 
ACATGCAACC AAGACCTGAA CGAGTGTGGC CTGAAGCCTC GTCCTTGTAA GCACCGCTGC 
ATGAACACAT TCGGGAGCTT CAAGTGTTAC TGTCTGAATG GCTTCATGCT GCTGCCGGAT 
GGGAGCTGCG CCAATGCCCG AACTTGCAGC ATGGCGAACT GCCAGTATGG CTGTGAGGTA 
ATGAAAGGAG AGGTTCGCTG CCAGTGCCCG TCTCCAGGTC TACAGCTGGC TCCAGATGGC 
AGGACCTGTG TGGATGTGGA TGAATGTGCC GCAGGTCTTG CTGTTTGTCC CCGTTTCCGG 
AAGTGCATCA ACACATTTGG GAGTTACATC TGCAAGTGCC ATGACGGCTT TGACCTTCAG 
TACGTCAATG GGAAATATCA GTGTACAGAT GTCAATGAAT GCTCTTTGGG TCAGCACCAG 
TGTGGTCCAT ACGCCACCTG TTATAACACA CCTGGTTCAT ACAAGTGCAA GTGTAAAGAA 
GACTACAGAG GCGTGGGCTA CGACTGCAAA CCCATCCCAA AGGTGGTAAT TGACCCTCCA 
CGACCTGGAA AGACCACACC GAGCAGCAAT AACAACAAAG GCGGCAACAA AATTCCAGGA 
TCAGACCAAA AGAGGACCAC CACAACAGTA AGACCTCCAG TAACGGCCAA ACGCATCTCA 
CCTACAATCA CCACCACCAC CACTACCACC AAACCTCCTC CAACCAAAAA AATCACCCCT 
CCAGCAAGAG TCCCGGTGAC CACCACCCGT AAGCCATTCA TCCCAACAAG GAAGCCCCCT 
GTGCTGGTGA CAACCAAACC TAAGGTCCCG ACTCACCAGC ACACCACCAC AAAAGTCCCA 
GTGGTTACAG CAGTGGTTCC TTTTATCCCA ACACGCAGAC CTTTCACCAT CCCGTTTGTG 
ACACCAATTG ATAACAGCAT CAAAGACATC ACCCAGAAAC AAAGAGGCGA TGTTCACATA 
CCACGAAATC ATGGGAAGAA CAATGTTCTT GGTATCGATT TAGACATTGA ACTGGGCAAC 
ACCGAAGAGG AGCTGAAGGA TGACCCAGAG TCTGGCTATC TGAGCTGCTC ATTTGATCAC 
GGTTTGTGTG GATGGATCCA GCGCAGAGAG GGAGACCTTC ATTGGGAGAC ATCGGAAGAT 
CCTTCGGGTG GGAGGTACCT AACCATCTCT GAGGGCGGGG AGAAGCGTGG TGGGCGTGGT 
GCTCAGCTGA TCCTCCCCCT GAAAACCCCC TGGAACGAGG GGAACCTGTG CTTGGCCTTC 
AGACACAACA TGGCAGGTCA CCATGTGGGA ATGCTGCAGG TGTTCGTACA GAAAGGTCGG 
CAGCACAGTC CCGCTGTCTG GGGACGAACA GGAGGAAACG GCTGGAGGTC CACACAGATC 
ACCCTTTGGG GCAATGGGCT AGAAAGTGTG ATCGTGAAGG GCGAGCGGCG GAGAGGCCGC 
AAGGGCGAAA TTGCTCTGGA TGACATGAGT CTCAAACGAG GTTCCTGTCA GGAAGAGCAC 
AATCTGAGGA GACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npnt ( XM_005160098.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160155.1 Danio rerio nephronectin (npnt), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa06176
Clone ID Related Accession (Same CDS sequence)	XM_005160098.4 , XM_005160098.3
Accession Version	XM_005160098.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nephronectin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160098.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATCA TAAAGTTCAT GTTGATGTGG ACCTGCTGGA TCGCAGTGGA CGCGGACTTC 
GATGGCAGGT GGTCCCGACA GATGTCCTCA TCCAATGGTC TGTGTCGGTA CGGCGGCCGC 
ATTGACTGCT GCTGGGGCTG GACGAGAGTC TCCTGGGGCC AGTGCCAGCC GCTATGTCAG 
CACGGATGCA AGCACGGAGA ATGCGTGGGA CCCAACAAAT GCAAGTGCCA TCCAGGTTAT 
ACAGGAAAAA CATGCAACCA AGACGAGCGG GGCTTTGTTA GCCCTCCATT ATGGCACAGT 
CACGCTCCTG TTTTTGTTCC AATGGACCAC CAGCCGATCG CGGCGCCTTC AGAGGACCTG 
AACGAGTGTG GCCTGAAGCC TCGTCCTTGT AAGCACCGCT GCATGAACAC ATTCGGGAGC 
TTCAAGTGTT ACTGTCTGAA TGGCTTCATG CTGCTGCCGG ATGGGAGCTG CGCCAATGCC 
CGAACTTGCA GCATGGCGAA CTGCCAGTAT GGCTGTGAGG TAATGAAAGG AGAGGTTCGC 
TGCCAGTGCC CGTCTCCAGG TCTACAGCTG GCTCCAGATG GCAGGACCTG TGTGGATGTG 
GATGAATGTG CCGCAGGTCT TGCTGTTTGT CCCCGTTTCC GGAAGTGCAT CAACACATTT 
GGGAGTTACA TCTGCAAGTG CCATGACGGC TTTGACCTTC AGTACGTCAA TGGGAAATAT 
CAGTGTACAG ATGTCAATGA ATGCTCTTTG GGTCAGCACC AGTGTGGTCC ATACGCCACC 
TGTTATAACA CACCTGGTTC ATACAAGTGC AAGTGTAAAG AAGACTACAG AGGCGTGGGC 
TACGACTGCA AACCCATCCC AAAGGTGGTA ATTGACCCTC CACGACCTGG AAAGACCACA 
CCGAGCAGCA ATAACAACAA AGGCGGCAAC AAAATTCCAG GATCAGACCA AAAGAGGACC 
ACCACAACAG TAAGACCTCC AGTAACGGCC AAACGCATCT CACCTACAAT CACCACCACC 
ACCACTACCA CCAAACCTCC TCCAACCAAA AAAATCACCC CTCCAGCAAG AGTCCCGGTG 
ACCACCACCC GTAAGCCATT CATCCCAACA AGGAAGCCCC CTGTGCTGGT GACAACCAAA 
CCTAAGGTCC CGACTCACCA GCACACCACC ACAAAAGTCC CAGTGGTTAC AGCAGTGGTT 
CCTTTTATCC CAACACGCAG ACCTTTCACC ATCCCGTTTG TGACACCAAT TGATAACAGC 
ATCAAAGACA TCACCCAGAA ACAAAGAGGC GATGTTCACA TACCACGAAA TCATGGGAAG 
AACAATGTTC TTGGTATCGA TTTAGACATT GAACTGGGCA ACACCGAAGA GGAGCTGAAG 
GATGACCCAG AGTCTGGCTA TCTGAGCTGC TCATTTGATC ACGGTTTGTG TGGATGGATC 
CAGCGCAGAG AGGGAGACCT TCATTGGGAG ACATCGGAAG ATCCTTCGGG TGGGAGGTAC 
CTAACCATCT CTGAGGGCGG GGAGAAGCGT GGTGGGCGTG GTGCTCAGCT GATCCTCCCC 
CTGAAAACCC CCTGGAACGA GGGGAACCTG TGCTTGGCCT TCAGACACAA CATGGCAGGT 
CACCATGTGG GAATGCTGCA GGTGTTCGTA CAGAAAGGTC GGCAGCACAG TCCCGCTGTC 
TGGGGACGAA CAGGAGGAAA CGGCTGGAGG TCCACACAGA TCACCCTTTG GGGCAATGGG 
CTAGAAAGTG TGATCGTGAA GGGCGAGCGG CGGAGAGGCC GCAAGGGCGA AATTGCTCTG 
GATGACATGA GTCTCAAACG AGGTTCCTGT CAGGAAGAGC ACAATCTGAG GAGACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160155.1
CDS	227..2086
Translation	MWIIKFMLMWTCWIAVDADFDGRWSRQMSSSNGLCRYGGRIDCCWGWTRVSWGQCQPLCQHGCKHGECVGPNKCKCHPGYTGKTCNQDERGFVSPPLWHSHAPVFVPMDHQPIAAPSEDLNECGLKPRPCKHRCMNTFGSFKCYCLNGFMLLPDGSCANARTCSMANCQYGCEVMKGEVRCQCPSPGLQLAPDGRTCVDVDECAAGLAVCPRFRKCINTFGSYICKCHDGFDLQYVNGKYQCTDVNECSLGQHQCGPYATCYNTPGSYKCKCKEDYRGVGYDCKPIPKVVIDPPRPGKTTPSSNNNKGGNKIPGSDQKRTTTTVRPPVTAKRISPTITTTTTTTKPPPTKKITPPARVPVTTTRKPFIPTRKPPVLVTTKPKVPTHQHTTTKVPVVTAVVPFIPTRRPFTIPFVTPIDNSIKDITQKQRGDVHIPRNHGKNNVLGIDLDIELGNTEEELKDDPESGYLSCSFDHGLCGWIQRREGDLHWETSEDPSGGRYLTISEGGEKRGGRGAQLILPLKTPWNEGNLCLAFRHNMAGHHVGMLQVFVQKGRQHSPAVWGRTGGNGWRSTQITLWGNGLESVIVKGERRRGRKGEIALDDMSLKRGSCQEEHNLRRL

Target ORF information:

RefSeq Version	XM_005160098.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nephronectin (npnt), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160098.4

ORF Insert Sequence:

ATGTGGATCA TAAAGTTCAT GTTGATGTGG ACCTGCTGGA TCGCAGTGGA CGCGGACTTC 
GATGGCAGGT GGTCCCGACA GATGTCCTCA TCCAATGGTC TGTGTCGGTA CGGCGGCCGC 
ATTGACTGCT GCTGGGGCTG GACGAGAGTC TCCTGGGGCC AGTGCCAGCC GCTATGTCAG 
CACGGATGCA AGCACGGAGA ATGCGTGGGA CCCAACAAAT GCAAGTGCCA TCCAGGTTAT 
ACAGGAAAAA CATGCAACCA AGACGAGCGG GGCTTTGTTA GCCCTCCATT ATGGCACAGT 
CACGCTCCTG TTTTTGTTCC AATGGACCAC CAGCCGATCG CGGCGCCTTC AGAGGACCTG 
AACGAGTGTG GCCTGAAGCC TCGTCCTTGT AAGCACCGCT GCATGAACAC ATTCGGGAGC 
TTCAAGTGTT ACTGTCTGAA TGGCTTCATG CTGCTGCCGG ATGGGAGCTG CGCCAATGCC 
CGAACTTGCA GCATGGCGAA CTGCCAGTAT GGCTGTGAGG TAATGAAAGG AGAGGTTCGC 
TGCCAGTGCC CGTCTCCAGG TCTACAGCTG GCTCCAGATG GCAGGACCTG TGTGGATGTG 
GATGAATGTG CCGCAGGTCT TGCTGTTTGT CCCCGTTTCC GGAAGTGCAT CAACACATTT 
GGGAGTTACA TCTGCAAGTG CCATGACGGC TTTGACCTTC AGTACGTCAA TGGGAAATAT 
CAGTGTACAG ATGTCAATGA ATGCTCTTTG GGTCAGCACC AGTGTGGTCC ATACGCCACC 
TGTTATAACA CACCTGGTTC ATACAAGTGC AAGTGTAAAG AAGACTACAG AGGCGTGGGC 
TACGACTGCA AACCCATCCC AAAGGTGGTA ATTGACCCTC CACGACCTGG AAAGACCACA 
CCGAGCAGCA ATAACAACAA AGGCGGCAAC AAAATTCCAG GATCAGACCA AAAGAGGACC 
ACCACAACAG TAAGACCTCC AGTAACGGCC AAACGCATCT CACCTACAAT CACCACCACC 
ACCACTACCA CCAAACCTCC TCCAACCAAA AAAATCACCC CTCCAGCAAG AGTCCCGGTG 
ACCACCACCC GTAAGCCATT CATCCCAACA AGGAAGCCCC CTGTGCTGGT GACAACCAAA 
CCTAAGGTCC CGACTCACCA GCACACCACC ACAAAAGTCC CAGTGGTTAC AGCAGTGGTT 
CCTTTTATCC CAACACGCAG ACCTTTCACC ATCCCGTTTG TGACACCAAT TGATAACAGC 
ATCAAAGACA TCACCCAGAA ACAAAGAGGC GATGTTCACA TACCACGAAA TCATGGGAAG 
AACAATGTTC TTGGTATCGA TTTAGACATT GAACTGGGCA ACACCGAAGA GGAGCTGAAG 
GATGACCCAG AGTCTGGCTA TCTGAGCTGC TCATTTGATC ACGGTTTGTG TGGATGGATC 
CAGCGCAGAG AGGGAGACCT TCATTGGGAG ACATCGGAAG ATCCTTCGGG TGGGAGGTAC 
CTAACCATCT CTGAGGGCGG GGAGAAGCGT GGTGGGCGTG GTGCTCAGCT GATCCTCCCC 
CTGAAAACCC CCTGGAACGA GGGGAACCTG TGCTTGGCCT TCAGACACAA CATGGCAGGT 
CACCATGTGG GAATGCTGCA GGTGTTCGTA CAGAAAGGTC GGCAGCACAG TCCCGCTGTC 
TGGGGACGAA CAGGAGGAAA CGGCTGGAGG TCCACACAGA TCACCCTTTG GGGCAATGGG 
CTAGAAAGTG TGATCGTGAA GGGCGAGCGG CGGAGAGGCC GCAAGGGCGA AATTGCTCTG 
GATGACATGA GTCTCAAACG AGGTTCCTGT CAGGAAGAGC ACAATCTGAG GAGACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npnt ( XM_005160098.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160155.1 Danio rerio nephronectin (npnt), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa06176
Clone ID Related Accession (Same CDS sequence)	XM_005160098.4 , XM_005160098.3
Accession Version	XM_005160098.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nephronectin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160098.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATCA TAAAGTTCAT GTTGATGTGG ACCTGCTGGA TCGCAGTGGA CGCGGACTTC 
GATGGCAGGT GGTCCCGACA GATGTCCTCA TCCAATGGTC TGTGTCGGTA CGGCGGCCGC 
ATTGACTGCT GCTGGGGCTG GACGAGAGTC TCCTGGGGCC AGTGCCAGCC GCTATGTCAG 
CACGGATGCA AGCACGGAGA ATGCGTGGGA CCCAACAAAT GCAAGTGCCA TCCAGGTTAT 
ACAGGAAAAA CATGCAACCA AGACGAGCGG GGCTTTGTTA GCCCTCCATT ATGGCACAGT 
CACGCTCCTG TTTTTGTTCC AATGGACCAC CAGCCGATCG CGGCGCCTTC AGAGGACCTG 
AACGAGTGTG GCCTGAAGCC TCGTCCTTGT AAGCACCGCT GCATGAACAC ATTCGGGAGC 
TTCAAGTGTT ACTGTCTGAA TGGCTTCATG CTGCTGCCGG ATGGGAGCTG CGCCAATGCC 
CGAACTTGCA GCATGGCGAA CTGCCAGTAT GGCTGTGAGG TAATGAAAGG AGAGGTTCGC 
TGCCAGTGCC CGTCTCCAGG TCTACAGCTG GCTCCAGATG GCAGGACCTG TGTGGATGTG 
GATGAATGTG CCGCAGGTCT TGCTGTTTGT CCCCGTTTCC GGAAGTGCAT CAACACATTT 
GGGAGTTACA TCTGCAAGTG CCATGACGGC TTTGACCTTC AGTACGTCAA TGGGAAATAT 
CAGTGTACAG ATGTCAATGA ATGCTCTTTG GGTCAGCACC AGTGTGGTCC ATACGCCACC 
TGTTATAACA CACCTGGTTC ATACAAGTGC AAGTGTAAAG AAGACTACAG AGGCGTGGGC 
TACGACTGCA AACCCATCCC AAAGGTGGTA ATTGACCCTC CACGACCTGG AAAGACCACA 
CCGAGCAGCA ATAACAACAA AGGCGGCAAC AAAATTCCAG GATCAGACCA AAAGAGGACC 
ACCACAACAG TAAGACCTCC AGTAACGGCC AAACGCATCT CACCTACAAT CACCACCACC 
ACCACTACCA CCAAACCTCC TCCAACCAAA AAAATCACCC CTCCAGCAAG AGTCCCGGTG 
ACCACCACCC GTAAGCCATT CATCCCAACA AGGAAGCCCC CTGTGCTGGT GACAACCAAA 
CCTAAGGTCC CGACTCACCA GCACACCACC ACAAAAGTCC CAGTGGTTAC AGCAGTGGTT 
CCTTTTATCC CAACACGCAG ACCTTTCACC ATCCCGTTTG TGACACCAAT TGATAACAGC 
ATCAAAGACA TCACCCAGAA ACAAAGAGGC GATGTTCACA TACCACGAAA TCATGGGAAG 
AACAATGTTC TTGGTATCGA TTTAGACATT GAACTGGGCA ACACCGAAGA GGAGCTGAAG 
GATGACCCAG AGTCTGGCTA TCTGAGCTGC TCATTTGATC ACGGTTTGTG TGGATGGATC 
CAGCGCAGAG AGGGAGACCT TCATTGGGAG ACATCGGAAG ATCCTTCGGG TGGGAGGTAC 
CTAACCATCT CTGAGGGCGG GGAGAAGCGT GGTGGGCGTG GTGCTCAGCT GATCCTCCCC 
CTGAAAACCC CCTGGAACGA GGGGAACCTG TGCTTGGCCT TCAGACACAA CATGGCAGGT 
CACCATGTGG GAATGCTGCA GGTGTTCGTA CAGAAAGGTC GGCAGCACAG TCCCGCTGTC 
TGGGGACGAA CAGGAGGAAA CGGCTGGAGG TCCACACAGA TCACCCTTTG GGGCAATGGG 
CTAGAAAGTG TGATCGTGAA GGGCGAGCGG CGGAGAGGCC GCAAGGGCGA AATTGCTCTG 
GATGACATGA GTCTCAAACG AGGTTCCTGT CAGGAAGAGC ACAATCTGAG GAGACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160155.1
CDS	241..2100
Translation	MWIIKFMLMWTCWIAVDADFDGRWSRQMSSSNGLCRYGGRIDCCWGWTRVSWGQCQPLCQHGCKHGECVGPNKCKCHPGYTGKTCNQDERGFVSPPLWHSHAPVFVPMDHQPIAAPSEDLNECGLKPRPCKHRCMNTFGSFKCYCLNGFMLLPDGSCANARTCSMANCQYGCEVMKGEVRCQCPSPGLQLAPDGRTCVDVDECAAGLAVCPRFRKCINTFGSYICKCHDGFDLQYVNGKYQCTDVNECSLGQHQCGPYATCYNTPGSYKCKCKEDYRGVGYDCKPIPKVVIDPPRPGKTTPSSNNNKGGNKIPGSDQKRTTTTVRPPVTAKRISPTITTTTTTTKPPPTKKITPPARVPVTTTRKPFIPTRKPPVLVTTKPKVPTHQHTTTKVPVVTAVVPFIPTRRPFTIPFVTPIDNSIKDITQKQRGDVHIPRNHGKNNVLGIDLDIELGNTEEELKDDPESGYLSCSFDHGLCGWIQRREGDLHWETSEDPSGGRYLTISEGGEKRGGRGAQLILPLKTPWNEGNLCLAFRHNMAGHHVGMLQVFVQKGRQHSPAVWGRTGGNGWRSTQITLWGNGLESVIVKGERRRGRKGEIALDDMSLKRGSCQEEHNLRRL

Target ORF information:

RefSeq Version	XM_005160098.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nephronectin (npnt), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160098.3

ORF Insert Sequence:

ATGTGGATCA TAAAGTTCAT GTTGATGTGG ACCTGCTGGA TCGCAGTGGA CGCGGACTTC 
GATGGCAGGT GGTCCCGACA GATGTCCTCA TCCAATGGTC TGTGTCGGTA CGGCGGCCGC 
ATTGACTGCT GCTGGGGCTG GACGAGAGTC TCCTGGGGCC AGTGCCAGCC GCTATGTCAG 
CACGGATGCA AGCACGGAGA ATGCGTGGGA CCCAACAAAT GCAAGTGCCA TCCAGGTTAT 
ACAGGAAAAA CATGCAACCA AGACGAGCGG GGCTTTGTTA GCCCTCCATT ATGGCACAGT 
CACGCTCCTG TTTTTGTTCC AATGGACCAC CAGCCGATCG CGGCGCCTTC AGAGGACCTG 
AACGAGTGTG GCCTGAAGCC TCGTCCTTGT AAGCACCGCT GCATGAACAC ATTCGGGAGC 
TTCAAGTGTT ACTGTCTGAA TGGCTTCATG CTGCTGCCGG ATGGGAGCTG CGCCAATGCC 
CGAACTTGCA GCATGGCGAA CTGCCAGTAT GGCTGTGAGG TAATGAAAGG AGAGGTTCGC 
TGCCAGTGCC CGTCTCCAGG TCTACAGCTG GCTCCAGATG GCAGGACCTG TGTGGATGTG 
GATGAATGTG CCGCAGGTCT TGCTGTTTGT CCCCGTTTCC GGAAGTGCAT CAACACATTT 
GGGAGTTACA TCTGCAAGTG CCATGACGGC TTTGACCTTC AGTACGTCAA TGGGAAATAT 
CAGTGTACAG ATGTCAATGA ATGCTCTTTG GGTCAGCACC AGTGTGGTCC ATACGCCACC 
TGTTATAACA CACCTGGTTC ATACAAGTGC AAGTGTAAAG AAGACTACAG AGGCGTGGGC 
TACGACTGCA AACCCATCCC AAAGGTGGTA ATTGACCCTC CACGACCTGG AAAGACCACA 
CCGAGCAGCA ATAACAACAA AGGCGGCAAC AAAATTCCAG GATCAGACCA AAAGAGGACC 
ACCACAACAG TAAGACCTCC AGTAACGGCC AAACGCATCT CACCTACAAT CACCACCACC 
ACCACTACCA CCAAACCTCC TCCAACCAAA AAAATCACCC CTCCAGCAAG AGTCCCGGTG 
ACCACCACCC GTAAGCCATT CATCCCAACA AGGAAGCCCC CTGTGCTGGT GACAACCAAA 
CCTAAGGTCC CGACTCACCA GCACACCACC ACAAAAGTCC CAGTGGTTAC AGCAGTGGTT 
CCTTTTATCC CAACACGCAG ACCTTTCACC ATCCCGTTTG TGACACCAAT TGATAACAGC 
ATCAAAGACA TCACCCAGAA ACAAAGAGGC GATGTTCACA TACCACGAAA TCATGGGAAG 
AACAATGTTC TTGGTATCGA TTTAGACATT GAACTGGGCA ACACCGAAGA GGAGCTGAAG 
GATGACCCAG AGTCTGGCTA TCTGAGCTGC TCATTTGATC ACGGTTTGTG TGGATGGATC 
CAGCGCAGAG AGGGAGACCT TCATTGGGAG ACATCGGAAG ATCCTTCGGG TGGGAGGTAC 
CTAACCATCT CTGAGGGCGG GGAGAAGCGT GGTGGGCGTG GTGCTCAGCT GATCCTCCCC 
CTGAAAACCC CCTGGAACGA GGGGAACCTG TGCTTGGCCT TCAGACACAA CATGGCAGGT 
CACCATGTGG GAATGCTGCA GGTGTTCGTA CAGAAAGGTC GGCAGCACAG TCCCGCTGTC 
TGGGGACGAA CAGGAGGAAA CGGCTGGAGG TCCACACAGA TCACCCTTTG GGGCAATGGG 
CTAGAAAGTG TGATCGTGAA GGGCGAGCGG CGGAGAGGCC GCAAGGGCGA AATTGCTCTG 
GATGACATGA GTCTCAAACG AGGTTCCTGT CAGGAAGAGC ACAATCTGAG GAGACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npnt ( XM_009294216.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292491.1 Danio rerio nephronectin (npnt), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa44023
Clone ID Related Accession (Same CDS sequence)	XM_009294216.2 , XM_009294216.3
Accession Version	XM_009294216.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nephronectin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294216.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATCA TAAAGTTCAT GTTGATGTGG ACCTGCTGGA TCGCAGTGGA CGCGGACTTC 
GATGGCAGGT GGTCCCGACA GATGTCCTCA TCCAATGGTC TGTGTCGGTA CGGCGGCCGC 
ATTGACTGCT GCTGGGGCTG GACGAGAGTC TCCTGGGGCC AGTGCCAGCC CCTCTACGTC 
TTAACCCGCA GAGTAATCGG GATAAGGTGT CAGCCCCAAG CGCTATGTCA GCACGGATGC 
AAGCACGGAG AATGCGTGGG ACCCAACAAA TGCAAGTGCC ATCCAGGTTA TACAGGAAAA 
ACATGCAACC AAGACGAGCG GGGCTTTGTT AGCCCTCCAT TATGGCACAG TCACGCTCCT 
GTTTTTGTTC CAATGGACCA CCAGCCGATC GCGGCGCCTT CAGAGGACCT GAACGAGTGT 
GGCCTGAAGC CTCGTCCTTG TAAGCACCGC TGCATGAACA CATTCGGGAG CTTCAAGTGT 
TACTGTCTGA ATGGCTTCAT GCTGCTGCCG GATGGGAGCT GCGCCAATGC CCGAACTTGC 
AGCATGGCGA ACTGCCAGTA TGGCTGTGAG GTAATGAAAG GAGAGGTTCG CTGCCAGTGC 
CCGTCTCCAG GTCTACAGCT GGCTCCAGAT GGCAGGACCT GTGTGGATGT GGATGAATGT 
GCCGCAGGTC TTGCTGTTTG TCCCCGTTTC CGGAAGTGCA TCAACACATT TGGGAGTTAC 
ATCTGCAAGT GCCATGACGG CTTTGACCTT CAGTACGTCA ATGGGAAATA TCAGTGTACA 
GATGTCAATG AATGCTCTTT GGGTCAGCAC CAGTGTGGTC CATACGCCAC CTGTTATAAC 
ACACCTGGTT CATACAAGTG CAAGTGTAAA GAAGACTACA GAGGCGTGGG CTACGACTGC 
AAACCCATCC CAAAGGTGGT AATTGACCCT CCACGACCTG GAAAGACCAC ACCGAGCAGC 
AATAACAACA AAGGCGGCAA CAAAATTCCA GGATCAGACC AAAAGAGGAC CACCACAACA 
GTAAGACCTC CAGTAACGGC CAAACGCATC TCACCTACAA TCACCACCAC CACCACTACC 
ACCAAACCTC CTCCAACCAA AAAAATCACC CCTCCAGCAA GAGTCCCGGT GACCACCACC 
CGTAAGCCAT TCATCCCAAC AAGGAAGCCC CCTGTGCTGG TGACAACCAA ACCTAAGGTC 
CCGACTCACC AGCACACCAC CACAAAAGTC CCAGTGGTTA CAGCAGTGGT TCCTTTTATC 
CCAACACGCA GACCTTTCAC CATCCCGTTT GTGACACCAA TTGATAACAG CATCAAAGAC 
ATCACCCAGA AACAAAGAGG CGATGTTCAC ATACCACGAA ATCATGGGAA GAACAATGTT 
CTTGGTATCG ATTTAGACAT TGAACTGGGC AACACCGAAG AGGAGCTGAA GGATGACCCA 
GAGTCTGGCT ATCTGAGCTG CTCATTTGAT CACGGTTTGT GTGGATGGAT CCAGCGCAGA 
GAGGGAGACC TTCATTGGGA GACATCGGAA GATCCTTCGG GTGGGAGGTA CCTAACCATC 
TCTGAGGGCG GGGAGAAGCG TGGTGGGCGT GGTGCTCAGC TGATCCTCCC CCTGAAAACC 
CCCTGGAACG AGGGGAACCT GTGCTTGGCC TTCAGACACA ACATGGCAGG TCACCATGTG 
GGAATGCTGC AGGTGTTCGT ACAGAAAGGT CGGCAGCACA GTCCCGCTGT CTGGGGACGA 
ACAGGAGGAA ACGGCTGGAG GTCCACACAG ATCACCCTTT GGGGCAATGG GCTAGAAAGT 
GTGATCGTGA AGGGCGAGCG GCGGAGAGGC CGCAAGGGCG AAATTGCTCT GGATGACATG 
AGTCTCAAAC GAGGTTCCTG TCAGGAAGAG CACAATCTGA GGAGACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292491.1
CDS	243..2153
Translation	MWIIKFMLMWTCWIAVDADFDGRWSRQMSSSNGLCRYGGRIDCCWGWTRVSWGQCQPLYVLTRRVIGIRCQPQALCQHGCKHGECVGPNKCKCHPGYTGKTCNQDERGFVSPPLWHSHAPVFVPMDHQPIAAPSEDLNECGLKPRPCKHRCMNTFGSFKCYCLNGFMLLPDGSCANARTCSMANCQYGCEVMKGEVRCQCPSPGLQLAPDGRTCVDVDECAAGLAVCPRFRKCINTFGSYICKCHDGFDLQYVNGKYQCTDVNECSLGQHQCGPYATCYNTPGSYKCKCKEDYRGVGYDCKPIPKVVIDPPRPGKTTPSSNNNKGGNKIPGSDQKRTTTTVRPPVTAKRISPTITTTTTTTKPPPTKKITPPARVPVTTTRKPFIPTRKPPVLVTTKPKVPTHQHTTTKVPVVTAVVPFIPTRRPFTIPFVTPIDNSIKDITQKQRGDVHIPRNHGKNNVLGIDLDIELGNTEEELKDDPESGYLSCSFDHGLCGWIQRREGDLHWETSEDPSGGRYLTISEGGEKRGGRGAQLILPLKTPWNEGNLCLAFRHNMAGHHVGMLQVFVQKGRQHSPAVWGRTGGNGWRSTQITLWGNGLESVIVKGERRRGRKGEIALDDMSLKRGSCQEEHNLRRL

Target ORF information:

RefSeq Version	XM_009294216.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nephronectin (npnt), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294216.2

ORF Insert Sequence:

ATGTGGATCA TAAAGTTCAT GTTGATGTGG ACCTGCTGGA TCGCAGTGGA CGCGGACTTC 
GATGGCAGGT GGTCCCGACA GATGTCCTCA TCCAATGGTC TGTGTCGGTA CGGCGGCCGC 
ATTGACTGCT GCTGGGGCTG GACGAGAGTC TCCTGGGGCC AGTGCCAGCC CCTCTACGTC 
TTAACCCGCA GAGTAATCGG GATAAGGTGT CAGCCCCAAG CGCTATGTCA GCACGGATGC 
AAGCACGGAG AATGCGTGGG ACCCAACAAA TGCAAGTGCC ATCCAGGTTA TACAGGAAAA 
ACATGCAACC AAGACGAGCG GGGCTTTGTT AGCCCTCCAT TATGGCACAG TCACGCTCCT 
GTTTTTGTTC CAATGGACCA CCAGCCGATC GCGGCGCCTT CAGAGGACCT GAACGAGTGT 
GGCCTGAAGC CTCGTCCTTG TAAGCACCGC TGCATGAACA CATTCGGGAG CTTCAAGTGT 
TACTGTCTGA ATGGCTTCAT GCTGCTGCCG GATGGGAGCT GCGCCAATGC CCGAACTTGC 
AGCATGGCGA ACTGCCAGTA TGGCTGTGAG GTAATGAAAG GAGAGGTTCG CTGCCAGTGC 
CCGTCTCCAG GTCTACAGCT GGCTCCAGAT GGCAGGACCT GTGTGGATGT GGATGAATGT 
GCCGCAGGTC TTGCTGTTTG TCCCCGTTTC CGGAAGTGCA TCAACACATT TGGGAGTTAC 
ATCTGCAAGT GCCATGACGG CTTTGACCTT CAGTACGTCA ATGGGAAATA TCAGTGTACA 
GATGTCAATG AATGCTCTTT GGGTCAGCAC CAGTGTGGTC CATACGCCAC CTGTTATAAC 
ACACCTGGTT CATACAAGTG CAAGTGTAAA GAAGACTACA GAGGCGTGGG CTACGACTGC 
AAACCCATCC CAAAGGTGGT AATTGACCCT CCACGACCTG GAAAGACCAC ACCGAGCAGC 
AATAACAACA AAGGCGGCAA CAAAATTCCA GGATCAGACC AAAAGAGGAC CACCACAACA 
GTAAGACCTC CAGTAACGGC CAAACGCATC TCACCTACAA TCACCACCAC CACCACTACC 
ACCAAACCTC CTCCAACCAA AAAAATCACC CCTCCAGCAA GAGTCCCGGT GACCACCACC 
CGTAAGCCAT TCATCCCAAC AAGGAAGCCC CCTGTGCTGG TGACAACCAA ACCTAAGGTC 
CCGACTCACC AGCACACCAC CACAAAAGTC CCAGTGGTTA CAGCAGTGGT TCCTTTTATC 
CCAACACGCA GACCTTTCAC CATCCCGTTT GTGACACCAA TTGATAACAG CATCAAAGAC 
ATCACCCAGA AACAAAGAGG CGATGTTCAC ATACCACGAA ATCATGGGAA GAACAATGTT 
CTTGGTATCG ATTTAGACAT TGAACTGGGC AACACCGAAG AGGAGCTGAA GGATGACCCA 
GAGTCTGGCT ATCTGAGCTG CTCATTTGAT CACGGTTTGT GTGGATGGAT CCAGCGCAGA 
GAGGGAGACC TTCATTGGGA GACATCGGAA GATCCTTCGG GTGGGAGGTA CCTAACCATC 
TCTGAGGGCG GGGAGAAGCG TGGTGGGCGT GGTGCTCAGC TGATCCTCCC CCTGAAAACC 
CCCTGGAACG AGGGGAACCT GTGCTTGGCC TTCAGACACA ACATGGCAGG TCACCATGTG 
GGAATGCTGC AGGTGTTCGT ACAGAAAGGT CGGCAGCACA GTCCCGCTGT CTGGGGACGA 
ACAGGAGGAA ACGGCTGGAG GTCCACACAG ATCACCCTTT GGGGCAATGG GCTAGAAAGT 
GTGATCGTGA AGGGCGAGCG GCGGAGAGGC CGCAAGGGCG AAATTGCTCT GGATGACATG 
AGTCTCAAAC GAGGTTCCTG TCAGGAAGAG CACAATCTGA GGAGACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npnt ( XM_009294216.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292491.1 Danio rerio nephronectin (npnt), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa44023
Clone ID Related Accession (Same CDS sequence)	XM_009294216.2 , XM_009294216.3
Accession Version	XM_009294216.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nephronectin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294216.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATCA TAAAGTTCAT GTTGATGTGG ACCTGCTGGA TCGCAGTGGA CGCGGACTTC 
GATGGCAGGT GGTCCCGACA GATGTCCTCA TCCAATGGTC TGTGTCGGTA CGGCGGCCGC 
ATTGACTGCT GCTGGGGCTG GACGAGAGTC TCCTGGGGCC AGTGCCAGCC CCTCTACGTC 
TTAACCCGCA GAGTAATCGG GATAAGGTGT CAGCCCCAAG CGCTATGTCA GCACGGATGC 
AAGCACGGAG AATGCGTGGG ACCCAACAAA TGCAAGTGCC ATCCAGGTTA TACAGGAAAA 
ACATGCAACC AAGACGAGCG GGGCTTTGTT AGCCCTCCAT TATGGCACAG TCACGCTCCT 
GTTTTTGTTC CAATGGACCA CCAGCCGATC GCGGCGCCTT CAGAGGACCT GAACGAGTGT 
GGCCTGAAGC CTCGTCCTTG TAAGCACCGC TGCATGAACA CATTCGGGAG CTTCAAGTGT 
TACTGTCTGA ATGGCTTCAT GCTGCTGCCG GATGGGAGCT GCGCCAATGC CCGAACTTGC 
AGCATGGCGA ACTGCCAGTA TGGCTGTGAG GTAATGAAAG GAGAGGTTCG CTGCCAGTGC 
CCGTCTCCAG GTCTACAGCT GGCTCCAGAT GGCAGGACCT GTGTGGATGT GGATGAATGT 
GCCGCAGGTC TTGCTGTTTG TCCCCGTTTC CGGAAGTGCA TCAACACATT TGGGAGTTAC 
ATCTGCAAGT GCCATGACGG CTTTGACCTT CAGTACGTCA ATGGGAAATA TCAGTGTACA 
GATGTCAATG AATGCTCTTT GGGTCAGCAC CAGTGTGGTC CATACGCCAC CTGTTATAAC 
ACACCTGGTT CATACAAGTG CAAGTGTAAA GAAGACTACA GAGGCGTGGG CTACGACTGC 
AAACCCATCC CAAAGGTGGT AATTGACCCT CCACGACCTG GAAAGACCAC ACCGAGCAGC 
AATAACAACA AAGGCGGCAA CAAAATTCCA GGATCAGACC AAAAGAGGAC CACCACAACA 
GTAAGACCTC CAGTAACGGC CAAACGCATC TCACCTACAA TCACCACCAC CACCACTACC 
ACCAAACCTC CTCCAACCAA AAAAATCACC CCTCCAGCAA GAGTCCCGGT GACCACCACC 
CGTAAGCCAT TCATCCCAAC AAGGAAGCCC CCTGTGCTGG TGACAACCAA ACCTAAGGTC 
CCGACTCACC AGCACACCAC CACAAAAGTC CCAGTGGTTA CAGCAGTGGT TCCTTTTATC 
CCAACACGCA GACCTTTCAC CATCCCGTTT GTGACACCAA TTGATAACAG CATCAAAGAC 
ATCACCCAGA AACAAAGAGG CGATGTTCAC ATACCACGAA ATCATGGGAA GAACAATGTT 
CTTGGTATCG ATTTAGACAT TGAACTGGGC AACACCGAAG AGGAGCTGAA GGATGACCCA 
GAGTCTGGCT ATCTGAGCTG CTCATTTGAT CACGGTTTGT GTGGATGGAT CCAGCGCAGA 
GAGGGAGACC TTCATTGGGA GACATCGGAA GATCCTTCGG GTGGGAGGTA CCTAACCATC 
TCTGAGGGCG GGGAGAAGCG TGGTGGGCGT GGTGCTCAGC TGATCCTCCC CCTGAAAACC 
CCCTGGAACG AGGGGAACCT GTGCTTGGCC TTCAGACACA ACATGGCAGG TCACCATGTG 
GGAATGCTGC AGGTGTTCGT ACAGAAAGGT CGGCAGCACA GTCCCGCTGT CTGGGGACGA 
ACAGGAGGAA ACGGCTGGAG GTCCACACAG ATCACCCTTT GGGGCAATGG GCTAGAAAGT 
GTGATCGTGA AGGGCGAGCG GCGGAGAGGC CGCAAGGGCG AAATTGCTCT GGATGACATG 
AGTCTCAAAC GAGGTTCCTG TCAGGAAGAG CACAATCTGA GGAGACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292491.1
CDS	227..2137
Translation	MWIIKFMLMWTCWIAVDADFDGRWSRQMSSSNGLCRYGGRIDCCWGWTRVSWGQCQPLYVLTRRVIGIRCQPQALCQHGCKHGECVGPNKCKCHPGYTGKTCNQDERGFVSPPLWHSHAPVFVPMDHQPIAAPSEDLNECGLKPRPCKHRCMNTFGSFKCYCLNGFMLLPDGSCANARTCSMANCQYGCEVMKGEVRCQCPSPGLQLAPDGRTCVDVDECAAGLAVCPRFRKCINTFGSYICKCHDGFDLQYVNGKYQCTDVNECSLGQHQCGPYATCYNTPGSYKCKCKEDYRGVGYDCKPIPKVVIDPPRPGKTTPSSNNNKGGNKIPGSDQKRTTTTVRPPVTAKRISPTITTTTTTTKPPPTKKITPPARVPVTTTRKPFIPTRKPPVLVTTKPKVPTHQHTTTKVPVVTAVVPFIPTRRPFTIPFVTPIDNSIKDITQKQRGDVHIPRNHGKNNVLGIDLDIELGNTEEELKDDPESGYLSCSFDHGLCGWIQRREGDLHWETSEDPSGGRYLTISEGGEKRGGRGAQLILPLKTPWNEGNLCLAFRHNMAGHHVGMLQVFVQKGRQHSPAVWGRTGGNGWRSTQITLWGNGLESVIVKGERRRGRKGEIALDDMSLKRGSCQEEHNLRRL

Target ORF information:

RefSeq Version	XM_009294216.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nephronectin (npnt), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294216.3

ORF Insert Sequence:

ATGTGGATCA TAAAGTTCAT GTTGATGTGG ACCTGCTGGA TCGCAGTGGA CGCGGACTTC 
GATGGCAGGT GGTCCCGACA GATGTCCTCA TCCAATGGTC TGTGTCGGTA CGGCGGCCGC 
ATTGACTGCT GCTGGGGCTG GACGAGAGTC TCCTGGGGCC AGTGCCAGCC CCTCTACGTC 
TTAACCCGCA GAGTAATCGG GATAAGGTGT CAGCCCCAAG CGCTATGTCA GCACGGATGC 
AAGCACGGAG AATGCGTGGG ACCCAACAAA TGCAAGTGCC ATCCAGGTTA TACAGGAAAA 
ACATGCAACC AAGACGAGCG GGGCTTTGTT AGCCCTCCAT TATGGCACAG TCACGCTCCT 
GTTTTTGTTC CAATGGACCA CCAGCCGATC GCGGCGCCTT CAGAGGACCT GAACGAGTGT 
GGCCTGAAGC CTCGTCCTTG TAAGCACCGC TGCATGAACA CATTCGGGAG CTTCAAGTGT 
TACTGTCTGA ATGGCTTCAT GCTGCTGCCG GATGGGAGCT GCGCCAATGC CCGAACTTGC 
AGCATGGCGA ACTGCCAGTA TGGCTGTGAG GTAATGAAAG GAGAGGTTCG CTGCCAGTGC 
CCGTCTCCAG GTCTACAGCT GGCTCCAGAT GGCAGGACCT GTGTGGATGT GGATGAATGT 
GCCGCAGGTC TTGCTGTTTG TCCCCGTTTC CGGAAGTGCA TCAACACATT TGGGAGTTAC 
ATCTGCAAGT GCCATGACGG CTTTGACCTT CAGTACGTCA ATGGGAAATA TCAGTGTACA 
GATGTCAATG AATGCTCTTT GGGTCAGCAC CAGTGTGGTC CATACGCCAC CTGTTATAAC 
ACACCTGGTT CATACAAGTG CAAGTGTAAA GAAGACTACA GAGGCGTGGG CTACGACTGC 
AAACCCATCC CAAAGGTGGT AATTGACCCT CCACGACCTG GAAAGACCAC ACCGAGCAGC 
AATAACAACA AAGGCGGCAA CAAAATTCCA GGATCAGACC AAAAGAGGAC CACCACAACA 
GTAAGACCTC CAGTAACGGC CAAACGCATC TCACCTACAA TCACCACCAC CACCACTACC 
ACCAAACCTC CTCCAACCAA AAAAATCACC CCTCCAGCAA GAGTCCCGGT GACCACCACC 
CGTAAGCCAT TCATCCCAAC AAGGAAGCCC CCTGTGCTGG TGACAACCAA ACCTAAGGTC 
CCGACTCACC AGCACACCAC CACAAAAGTC CCAGTGGTTA CAGCAGTGGT TCCTTTTATC 
CCAACACGCA GACCTTTCAC CATCCCGTTT GTGACACCAA TTGATAACAG CATCAAAGAC 
ATCACCCAGA AACAAAGAGG CGATGTTCAC ATACCACGAA ATCATGGGAA GAACAATGTT 
CTTGGTATCG ATTTAGACAT TGAACTGGGC AACACCGAAG AGGAGCTGAA GGATGACCCA 
GAGTCTGGCT ATCTGAGCTG CTCATTTGAT CACGGTTTGT GTGGATGGAT CCAGCGCAGA 
GAGGGAGACC TTCATTGGGA GACATCGGAA GATCCTTCGG GTGGGAGGTA CCTAACCATC 
TCTGAGGGCG GGGAGAAGCG TGGTGGGCGT GGTGCTCAGC TGATCCTCCC CCTGAAAACC 
CCCTGGAACG AGGGGAACCT GTGCTTGGCC TTCAGACACA ACATGGCAGG TCACCATGTG 
GGAATGCTGC AGGTGTTCGT ACAGAAAGGT CGGCAGCACA GTCCCGCTGT CTGGGGACGA 
ACAGGAGGAA ACGGCTGGAG GTCCACACAG ATCACCCTTT GGGGCAATGG GCTAGAAAGT 
GTGATCGTGA AGGGCGAGCG GCGGAGAGGC CGCAAGGGCG AAATTGCTCT GGATGACATG 
AGTCTCAAAC GAGGTTCCTG TCAGGAAGAG CACAATCTGA GGAGACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npnt ( XM_009294219.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292494.1 Danio rerio nephronectin (npnt), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa44024
Clone ID Related Accession (Same CDS sequence)	XM_009294219.3 , XM_009294219.2
Accession Version	XM_009294219.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nephronectin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294219.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATCA TAAAGTTCAT GTTGATGTGG ACCTGCTGGA TCGCAGTGGA CGCGGACTTC 
GATGGCAGGT GGTCCCGACA GATGTCCTCA TCCAATGGTC TGTGTCGGTA CGGCGGCCGC 
ATTGACTGCT GCTGGGGCTG GACGAGAGTC TCCTGGGGCC AGTGCCAGCC GCTATGTCAG 
CACGGATGCA AGCACGGAGA ATGCGTGGGA CCCAACAAAT GCAAGTGCCA TCCAGGTTAT 
ACAGGAAAAA CATGCAACCA AGACCTGAAC GAGTGTGGCC TGAAGCCTCG TCCTTGTAAG 
CACCGCTGCA TGAACACATT CGGGAGCTTC AAGTGTTACT GTCTGAATGG CTTCATGCTG 
CTGCCGGATG GGAGCTGCGC CAATGCCCGA ACTTGCAGCA TGGCGAACTG CCAGTATGGC 
TGTGAGGTAA TGAAAGGAGA GGTTCGCTGC CAGTGCCCGT CTCCAGGTCT ACAGCTGGCT 
CCAGATGGCA GGACCTGTGT GGATGTGGAT GAATGTGCCG CAGGTCTTGC TGTTTGTCCC 
CGTTTCCGGA AGTGCATCAA CACATTTGGG AGTTACATCT GCAAGTGCCA TGACGGCTTT 
GACCTTCAGT ACGTCAATGG GAAATATCAG TGTACAGATG TCAATGAATG CTCTTTGGGT 
CAGCACCAGT GTGGTCCATA CGCCACCTGT TATAACACAC CTGGTTCATA CAAGTGCAAG 
TGTAAAGAAG ACTACAGAGG CGTGGGCTAC GACTGCAAAC CCATCCCAAA GGTGGTAATT 
GACCCTCCAC GACCTGGAAA GACCACACCG AGCAGCAATA ACAACAAAGG CGGCAACAAA 
ATTCCAGGAT CAGACCAAAA GAGGACCACC ACAACAGTAA GACCTCCAGT AACGGCCAAA 
CGCATCTCAC CTACAATCAC CACCACCACC ACTACCACCA AACCTCCTCC AACCAAAAAA 
ATCACCCCTC CAGCAAGAGT CCCGGTGACC ACCACCCGTA AGCCATTCAT CCCAACAAGG 
AAGCCCCCTG TGCTGGTGAC AACCAAACCT AAGGTCCCGA CTCACCAGCA CACCACCACA 
AAAGTCCCAG TGGTTACAGC AGTGGTTCCT TTTATCCCAA CACGCAGACC TTTCACCATC 
CCGTTTGTGA CACCAATTGA TAACAGCATC AAAGACATCA CCCAGAAACA AAGAGGCGAT 
GTTCACATAC CACGAAATCA TGGGAAGAAC AATGTTCTTG GTATCGATTT AGACATTGAA 
CTGGGCAACA CCGAAGAGGA GCTGAAGGAT GACCCAGAGT CTGGCTATCT GAGCTGCTCA 
TTTGATCACG GTTTGTGTGG ATGGATCCAG CGCAGAGAGG GAGACCTTCA TTGGGAGACA 
TCGGAAGATC CTTCGGGTGG GAGGTACCTA ACCATCTCTG AGGGCGGGGA GAAGCGTGGT 
GGGCGTGGTG CTCAGCTGAT CCTCCCCCTG AAAACCCCCT GGAACGAGGG GAACCTGTGC 
TTGGCCTTCA GACACAACAT GGCAGGTCAC CATGTGGGAA TGCTGCAGGT GTTCGTACAG 
AAAGGTCGGC AGCACAGTCC CGCTGTCTGG GGACGAACAG GAGGAAACGG CTGGAGGTCC 
ACACAGATCA CCCTTTGGGG CAATGGGCTA GAAAGTGTGA TCGTGAAGGG CGAGCGGCGG 
AGAGGCCGCA AGGGCGAAAT TGCTCTGGAT GACATGAGTC TCAAACGAGG TTCCTGTCAG 
GAAGAGCACA ATCTGAGGAG ACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292494.1
CDS	226..1992
Translation	MWIIKFMLMWTCWIAVDADFDGRWSRQMSSSNGLCRYGGRIDCCWGWTRVSWGQCQPLCQHGCKHGECVGPNKCKCHPGYTGKTCNQDLNECGLKPRPCKHRCMNTFGSFKCYCLNGFMLLPDGSCANARTCSMANCQYGCEVMKGEVRCQCPSPGLQLAPDGRTCVDVDECAAGLAVCPRFRKCINTFGSYICKCHDGFDLQYVNGKYQCTDVNECSLGQHQCGPYATCYNTPGSYKCKCKEDYRGVGYDCKPIPKVVIDPPRPGKTTPSSNNNKGGNKIPGSDQKRTTTTVRPPVTAKRISPTITTTTTTTKPPPTKKITPPARVPVTTTRKPFIPTRKPPVLVTTKPKVPTHQHTTTKVPVVTAVVPFIPTRRPFTIPFVTPIDNSIKDITQKQRGDVHIPRNHGKNNVLGIDLDIELGNTEEELKDDPESGYLSCSFDHGLCGWIQRREGDLHWETSEDPSGGRYLTISEGGEKRGGRGAQLILPLKTPWNEGNLCLAFRHNMAGHHVGMLQVFVQKGRQHSPAVWGRTGGNGWRSTQITLWGNGLESVIVKGERRRGRKGEIALDDMSLKRGSCQEEHNLRRL

Target ORF information:

RefSeq Version	XM_009294219.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nephronectin (npnt), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294219.3

ORF Insert Sequence:

ATGTGGATCA TAAAGTTCAT GTTGATGTGG ACCTGCTGGA TCGCAGTGGA CGCGGACTTC 
GATGGCAGGT GGTCCCGACA GATGTCCTCA TCCAATGGTC TGTGTCGGTA CGGCGGCCGC 
ATTGACTGCT GCTGGGGCTG GACGAGAGTC TCCTGGGGCC AGTGCCAGCC GCTATGTCAG 
CACGGATGCA AGCACGGAGA ATGCGTGGGA CCCAACAAAT GCAAGTGCCA TCCAGGTTAT 
ACAGGAAAAA CATGCAACCA AGACCTGAAC GAGTGTGGCC TGAAGCCTCG TCCTTGTAAG 
CACCGCTGCA TGAACACATT CGGGAGCTTC AAGTGTTACT GTCTGAATGG CTTCATGCTG 
CTGCCGGATG GGAGCTGCGC CAATGCCCGA ACTTGCAGCA TGGCGAACTG CCAGTATGGC 
TGTGAGGTAA TGAAAGGAGA GGTTCGCTGC CAGTGCCCGT CTCCAGGTCT ACAGCTGGCT 
CCAGATGGCA GGACCTGTGT GGATGTGGAT GAATGTGCCG CAGGTCTTGC TGTTTGTCCC 
CGTTTCCGGA AGTGCATCAA CACATTTGGG AGTTACATCT GCAAGTGCCA TGACGGCTTT 
GACCTTCAGT ACGTCAATGG GAAATATCAG TGTACAGATG TCAATGAATG CTCTTTGGGT 
CAGCACCAGT GTGGTCCATA CGCCACCTGT TATAACACAC CTGGTTCATA CAAGTGCAAG 
TGTAAAGAAG ACTACAGAGG CGTGGGCTAC GACTGCAAAC CCATCCCAAA GGTGGTAATT 
GACCCTCCAC GACCTGGAAA GACCACACCG AGCAGCAATA ACAACAAAGG CGGCAACAAA 
ATTCCAGGAT CAGACCAAAA GAGGACCACC ACAACAGTAA GACCTCCAGT AACGGCCAAA 
CGCATCTCAC CTACAATCAC CACCACCACC ACTACCACCA AACCTCCTCC AACCAAAAAA 
ATCACCCCTC CAGCAAGAGT CCCGGTGACC ACCACCCGTA AGCCATTCAT CCCAACAAGG 
AAGCCCCCTG TGCTGGTGAC AACCAAACCT AAGGTCCCGA CTCACCAGCA CACCACCACA 
AAAGTCCCAG TGGTTACAGC AGTGGTTCCT TTTATCCCAA CACGCAGACC TTTCACCATC 
CCGTTTGTGA CACCAATTGA TAACAGCATC AAAGACATCA CCCAGAAACA AAGAGGCGAT 
GTTCACATAC CACGAAATCA TGGGAAGAAC AATGTTCTTG GTATCGATTT AGACATTGAA 
CTGGGCAACA CCGAAGAGGA GCTGAAGGAT GACCCAGAGT CTGGCTATCT GAGCTGCTCA 
TTTGATCACG GTTTGTGTGG ATGGATCCAG CGCAGAGAGG GAGACCTTCA TTGGGAGACA 
TCGGAAGATC CTTCGGGTGG GAGGTACCTA ACCATCTCTG AGGGCGGGGA GAAGCGTGGT 
GGGCGTGGTG CTCAGCTGAT CCTCCCCCTG AAAACCCCCT GGAACGAGGG GAACCTGTGC 
TTGGCCTTCA GACACAACAT GGCAGGTCAC CATGTGGGAA TGCTGCAGGT GTTCGTACAG 
AAAGGTCGGC AGCACAGTCC CGCTGTCTGG GGACGAACAG GAGGAAACGG CTGGAGGTCC 
ACACAGATCA CCCTTTGGGG CAATGGGCTA GAAAGTGTGA TCGTGAAGGG CGAGCGGCGG 
AGAGGCCGCA AGGGCGAAAT TGCTCTGGAT GACATGAGTC TCAAACGAGG TTCCTGTCAG 
GAAGAGCACA ATCTGAGGAG ACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npnt ( XM_009294219.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292494.1 Danio rerio nephronectin (npnt), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa44024
Clone ID Related Accession (Same CDS sequence)	XM_009294219.3 , XM_009294219.2
Accession Version	XM_009294219.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nephronectin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294219.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATCA TAAAGTTCAT GTTGATGTGG ACCTGCTGGA TCGCAGTGGA CGCGGACTTC 
GATGGCAGGT GGTCCCGACA GATGTCCTCA TCCAATGGTC TGTGTCGGTA CGGCGGCCGC 
ATTGACTGCT GCTGGGGCTG GACGAGAGTC TCCTGGGGCC AGTGCCAGCC GCTATGTCAG 
CACGGATGCA AGCACGGAGA ATGCGTGGGA CCCAACAAAT GCAAGTGCCA TCCAGGTTAT 
ACAGGAAAAA CATGCAACCA AGACCTGAAC GAGTGTGGCC TGAAGCCTCG TCCTTGTAAG 
CACCGCTGCA TGAACACATT CGGGAGCTTC AAGTGTTACT GTCTGAATGG CTTCATGCTG 
CTGCCGGATG GGAGCTGCGC CAATGCCCGA ACTTGCAGCA TGGCGAACTG CCAGTATGGC 
TGTGAGGTAA TGAAAGGAGA GGTTCGCTGC CAGTGCCCGT CTCCAGGTCT ACAGCTGGCT 
CCAGATGGCA GGACCTGTGT GGATGTGGAT GAATGTGCCG CAGGTCTTGC TGTTTGTCCC 
CGTTTCCGGA AGTGCATCAA CACATTTGGG AGTTACATCT GCAAGTGCCA TGACGGCTTT 
GACCTTCAGT ACGTCAATGG GAAATATCAG TGTACAGATG TCAATGAATG CTCTTTGGGT 
CAGCACCAGT GTGGTCCATA CGCCACCTGT TATAACACAC CTGGTTCATA CAAGTGCAAG 
TGTAAAGAAG ACTACAGAGG CGTGGGCTAC GACTGCAAAC CCATCCCAAA GGTGGTAATT 
GACCCTCCAC GACCTGGAAA GACCACACCG AGCAGCAATA ACAACAAAGG CGGCAACAAA 
ATTCCAGGAT CAGACCAAAA GAGGACCACC ACAACAGTAA GACCTCCAGT AACGGCCAAA 
CGCATCTCAC CTACAATCAC CACCACCACC ACTACCACCA AACCTCCTCC AACCAAAAAA 
ATCACCCCTC CAGCAAGAGT CCCGGTGACC ACCACCCGTA AGCCATTCAT CCCAACAAGG 
AAGCCCCCTG TGCTGGTGAC AACCAAACCT AAGGTCCCGA CTCACCAGCA CACCACCACA 
AAAGTCCCAG TGGTTACAGC AGTGGTTCCT TTTATCCCAA CACGCAGACC TTTCACCATC 
CCGTTTGTGA CACCAATTGA TAACAGCATC AAAGACATCA CCCAGAAACA AAGAGGCGAT 
GTTCACATAC CACGAAATCA TGGGAAGAAC AATGTTCTTG GTATCGATTT AGACATTGAA 
CTGGGCAACA CCGAAGAGGA GCTGAAGGAT GACCCAGAGT CTGGCTATCT GAGCTGCTCA 
TTTGATCACG GTTTGTGTGG ATGGATCCAG CGCAGAGAGG GAGACCTTCA TTGGGAGACA 
TCGGAAGATC CTTCGGGTGG GAGGTACCTA ACCATCTCTG AGGGCGGGGA GAAGCGTGGT 
GGGCGTGGTG CTCAGCTGAT CCTCCCCCTG AAAACCCCCT GGAACGAGGG GAACCTGTGC 
TTGGCCTTCA GACACAACAT GGCAGGTCAC CATGTGGGAA TGCTGCAGGT GTTCGTACAG 
AAAGGTCGGC AGCACAGTCC CGCTGTCTGG GGACGAACAG GAGGAAACGG CTGGAGGTCC 
ACACAGATCA CCCTTTGGGG CAATGGGCTA GAAAGTGTGA TCGTGAAGGG CGAGCGGCGG 
AGAGGCCGCA AGGGCGAAAT TGCTCTGGAT GACATGAGTC TCAAACGAGG TTCCTGTCAG 
GAAGAGCACA ATCTGAGGAG ACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292494.1
CDS	240..2006
Translation	MWIIKFMLMWTCWIAVDADFDGRWSRQMSSSNGLCRYGGRIDCCWGWTRVSWGQCQPLCQHGCKHGECVGPNKCKCHPGYTGKTCNQDLNECGLKPRPCKHRCMNTFGSFKCYCLNGFMLLPDGSCANARTCSMANCQYGCEVMKGEVRCQCPSPGLQLAPDGRTCVDVDECAAGLAVCPRFRKCINTFGSYICKCHDGFDLQYVNGKYQCTDVNECSLGQHQCGPYATCYNTPGSYKCKCKEDYRGVGYDCKPIPKVVIDPPRPGKTTPSSNNNKGGNKIPGSDQKRTTTTVRPPVTAKRISPTITTTTTTTKPPPTKKITPPARVPVTTTRKPFIPTRKPPVLVTTKPKVPTHQHTTTKVPVVTAVVPFIPTRRPFTIPFVTPIDNSIKDITQKQRGDVHIPRNHGKNNVLGIDLDIELGNTEEELKDDPESGYLSCSFDHGLCGWIQRREGDLHWETSEDPSGGRYLTISEGGEKRGGRGAQLILPLKTPWNEGNLCLAFRHNMAGHHVGMLQVFVQKGRQHSPAVWGRTGGNGWRSTQITLWGNGLESVIVKGERRRGRKGEIALDDMSLKRGSCQEEHNLRRL

Target ORF information:

RefSeq Version	XM_009294219.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nephronectin (npnt), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294219.2

ORF Insert Sequence:

ATGTGGATCA TAAAGTTCAT GTTGATGTGG ACCTGCTGGA TCGCAGTGGA CGCGGACTTC 
GATGGCAGGT GGTCCCGACA GATGTCCTCA TCCAATGGTC TGTGTCGGTA CGGCGGCCGC 
ATTGACTGCT GCTGGGGCTG GACGAGAGTC TCCTGGGGCC AGTGCCAGCC GCTATGTCAG 
CACGGATGCA AGCACGGAGA ATGCGTGGGA CCCAACAAAT GCAAGTGCCA TCCAGGTTAT 
ACAGGAAAAA CATGCAACCA AGACCTGAAC GAGTGTGGCC TGAAGCCTCG TCCTTGTAAG 
CACCGCTGCA TGAACACATT CGGGAGCTTC AAGTGTTACT GTCTGAATGG CTTCATGCTG 
CTGCCGGATG GGAGCTGCGC CAATGCCCGA ACTTGCAGCA TGGCGAACTG CCAGTATGGC 
TGTGAGGTAA TGAAAGGAGA GGTTCGCTGC CAGTGCCCGT CTCCAGGTCT ACAGCTGGCT 
CCAGATGGCA GGACCTGTGT GGATGTGGAT GAATGTGCCG CAGGTCTTGC TGTTTGTCCC 
CGTTTCCGGA AGTGCATCAA CACATTTGGG AGTTACATCT GCAAGTGCCA TGACGGCTTT 
GACCTTCAGT ACGTCAATGG GAAATATCAG TGTACAGATG TCAATGAATG CTCTTTGGGT 
CAGCACCAGT GTGGTCCATA CGCCACCTGT TATAACACAC CTGGTTCATA CAAGTGCAAG 
TGTAAAGAAG ACTACAGAGG CGTGGGCTAC GACTGCAAAC CCATCCCAAA GGTGGTAATT 
GACCCTCCAC GACCTGGAAA GACCACACCG AGCAGCAATA ACAACAAAGG CGGCAACAAA 
ATTCCAGGAT CAGACCAAAA GAGGACCACC ACAACAGTAA GACCTCCAGT AACGGCCAAA 
CGCATCTCAC CTACAATCAC CACCACCACC ACTACCACCA AACCTCCTCC AACCAAAAAA 
ATCACCCCTC CAGCAAGAGT CCCGGTGACC ACCACCCGTA AGCCATTCAT CCCAACAAGG 
AAGCCCCCTG TGCTGGTGAC AACCAAACCT AAGGTCCCGA CTCACCAGCA CACCACCACA 
AAAGTCCCAG TGGTTACAGC AGTGGTTCCT TTTATCCCAA CACGCAGACC TTTCACCATC 
CCGTTTGTGA CACCAATTGA TAACAGCATC AAAGACATCA CCCAGAAACA AAGAGGCGAT 
GTTCACATAC CACGAAATCA TGGGAAGAAC AATGTTCTTG GTATCGATTT AGACATTGAA 
CTGGGCAACA CCGAAGAGGA GCTGAAGGAT GACCCAGAGT CTGGCTATCT GAGCTGCTCA 
TTTGATCACG GTTTGTGTGG ATGGATCCAG CGCAGAGAGG GAGACCTTCA TTGGGAGACA 
TCGGAAGATC CTTCGGGTGG GAGGTACCTA ACCATCTCTG AGGGCGGGGA GAAGCGTGGT 
GGGCGTGGTG CTCAGCTGAT CCTCCCCCTG AAAACCCCCT GGAACGAGGG GAACCTGTGC 
TTGGCCTTCA GACACAACAT GGCAGGTCAC CATGTGGGAA TGCTGCAGGT GTTCGTACAG 
AAAGGTCGGC AGCACAGTCC CGCTGTCTGG GGACGAACAG GAGGAAACGG CTGGAGGTCC 
ACACAGATCA CCCTTTGGGG CAATGGGCTA GAAAGTGTGA TCGTGAAGGG CGAGCGGCGG 
AGAGGCCGCA AGGGCGAAAT TGCTCTGGAT GACATGAGTC TCAAACGAGG TTCCTGTCAG 
GAAGAGCACA ATCTGAGGAG ACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Podocytes regulate the glomerular basement membrane protein nephronectin by means of?miR-378a-3p in glomerular diseases.
Kidney international92(4)836-849(2017 Oct)
M?ller-Deile J,Dannenberg J,Schroder P,Lin MH,Miner JH,Chen R,Br?sen JH,Thum T,Nystr?m J,Staggs LB,Haller H,Fiedler J,Lorenzen JM,Schiffer M

Pharyngeal morphogenesis requires fras1-itga8-dependent epithelial-mesenchymal interaction.
Developmental biology416(1)136-148(2016 08)
Talbot JC,Nichols JT,Yan YL,Leonard IF,BreMiller RA,Amacher SL,Postlethwait JH,Kimmel CB

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Gene responses in the central nervous system of zebrafish embryos exposed to the neurotoxicant methyl mercury.
Environmental science & technology47(7)3316-25(2013 Apr)
Ho NY,Yang L,Legradi J,Armant O,Takamiya M,Rastegar S,Str?hle U

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npnt cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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