specc1 cDNA ORF clone, Danio rerio(zebrafish)

The following specc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the specc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa11132 XM_005168632.4
Latest version!
Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa11133 XM_005168633.4
Latest version!
Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa11131 XM_009301918.2
Latest version!
Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa11131 XM_009301917.3
Latest version!
Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa11131 XM_685735.8
Latest version!
Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa11131 XM_009301917.2 Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa11132 XM_005168632.3 Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa11131 XM_685735.7 Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa11133 XM_005168633.3 Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa11132
    Clone ID Related Accession (Same CDS sequence) XM_005168632.4 , XM_005168632.3
    Accession Version XM_005168632.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2787bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product cytospin-B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168632.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGGAACC AGGAAACCCG GGTGGAAGAT CCGGACACAG GTGTCCAGTC AACCAAAAAG 
    TCAGGGATCC CAGCTCCTCG TGAGATCCCC CAAAGCATCT CCAGAGAACG TCTCTCATCG
    CGCGATCAAC AGAAAAGCTC TACAGCCAAA AAGATGGTCA CCAGTTCCAG CGCCTCAAGC
    ATCTCTGCGC TGGCCAAGCA CTCACGTGGC TCTGTCAAGA CCAAAGCGGA GTCTGCGGCC
    TTGGACAAGA CGTTGCTGGA GAGTCAGGTG AGGGAGCTTC TGGCTGAGGC CAAAACCAAA
    GAGTTCGAGA TCAGTAAGCT ACGAACGGAG CTTCAACGGC ATAAAGGGAA GGATTCGCCA
    TCAGAGGCTG GGACACCAGA AGGGAATTCA GAGATGGAAA GGACACCGGC TCTGACTGGA
    GACGTCCAGG TGCTTGTAGG TGAACTTAGG GAGAAGAACG GACGTTTCCA ACGTGAGTTG
    GCCACTTTGC GGGAAGAGAA TCAATCTTTG AAGCAAAAGC TCATTGCATT GGAGAGCGCC
    AGCTCCAGCA AGCATTCTGT TAACGGAGTT GTCCCAGATA ACATCAATGG AAGCGGTCTT
    AAAACTTCAG TGTCATCTGC AAGTGACATC AAAGTCTCTC CTTTACCCTC TTTAGACTCC
    TCTGAGTTTG AGAAGATCCC CTCACGCTCA GACTCTGTCA GTAGCAGCAT TAAGAGCGTG
    GTGCCCAGCG GTGTGGTGAA AGATCTCTCT GTGGAGTCTC TGACAGAACG CATCCAGAAG
    ATGGAAGAGA GTCACCACAG TACGGCCGAA GAGCTTCAGG CCACTCTACA GGAGTTGGCT
    GACCAGCAAC AGGTCGTCCA GGAGCTGACA GCTGAGAATG AGCGTTTGGC AGAGGAGAAA
    GGCCTCTTGC AGACTTCCCT ACAGCAGCAA AGGGAGCGCG TAGAACTCTT GGCGCAACAA
    AACGAGACTC TTCTCCAGAG GCTCAGAGAA CAAGCTCAGA GTCAAGAAGC TGAGGCTTCA
    CGTGCCTCTC GGATGGCAGA ACTCGAACAG CGTTTGGCTG AGCAAGTAGA GAGTTCGCGA
    TTTGAAAGAG AGAAGCTGGT AGACATCCAG CAGCAGCTGA CAGGGAGTCT CAGAGCCTTG
    GAGAAGGAGA ATCAGGAGGC GCAAACTGCT GTGAAGAGCT TAAGGGAAGA AGAAGGGCTT
    CTGCAAGGGC ATCTGGAGAG TGAGAAGCTT GCTAGAGATG AGGCAGTGAG GAAAACTGAG
    GAACAGAGGC TAGCCATGGA GGCTCTGAGG GTGGACAATG CGAGTATGAA GGCGCAGGTG
    GAGGTGGAGC GGCAGAAGGT TGCTGAGCTG AAGGCAGTGC AGAGCGCTAG TGACAACACT
    GAGCTGCAAA GTCTGCTCAA AGTGGCGCAT GAAGATCGAG ACAAGCTGGA GATGACCTGC
    ACAGAGCTAC GACAAGAGCT GCAGCAGGTG GAGGCAGACA GACAGCAGCT TCTGGAGAAG
    GTGGAGAAAC AGGAACAAGA TCATAAAGCC AACACAAATT CTCTCGAGGA AAAGAGCATA
    GATGCTGAGA ACCAAATCAA GGACCTGAAA GAGACCATCT TCGAGTTGGA GGACCAGGTG
    GAGCAGCAAC GGGCTGTTAG ACTGCACACC AACCAGACCA TCCTGGACCT GGAGAATCAA
    ATCAAGAAGC TGGAAGACAA CAAAACTGAA CTGGAGAAGC AGATTAAGGT CGTGAATAAA
    CAAATGAAGG ATGAAACGGA GGAGTGGCGC CGTTTCCAGG CTGACCTCCA GACAGCAGTA
    GTGGTTGCCA ATGACATAAA GGTGGAGGCT CAGCAGGAGC TGCGGACGCT GCGCAGACGT
    CTGCAGGAGG AGCAGGAGCA CAGCGCGAGA CTGGCTTCAG AGCTGGAGCA GATCCAAGGC
    ACCAGGTCAT GGAATGAAGA TGCCAGCCTC TCCGACTCAG ACGGCAGTCC ACACTGGAGC
    CGAATGACCA TTTCCCAGAA TGCACCTGTG GGTTTCCTAA CCAATGGCAA TGGCAGTGGA
    AGCAGTGTGT CCTCAGAGCC AGAAGCCACG GTCAAATCTC TCATCAAATC ATTTGACACC
    GCAACGCCGA ATGGATCTAG CAGCTCAGTA CAGATACACA CATCTCCCAG AAGCCCCCTG
    AGTGGCATAC CAGTCCGTAC AGCTCCCGCT GCTGCCGTCT CACCCATTCA GAGGCACTCT
    GGAATCAAGC CACTGTCGAA GACCCTCGAG AGAAGAATTG CCTTTGGAGA TTTCCCACAC
    AATGGTTCAT GTGATGAACT GAAGCCCTCG ACTCTTATGA GGAAGAGCCC GTCTCTAGAG
    TCTGTGATTA AAACTCCAGC TCACTTCAAC GGCCGCACTG CATCGTTTAC TTACAACAGA
    GCCAACAGCA AACTCAGTGT GGAAAGAACA GACCCTCTGT CCGCTCTGGC GCGGGAGTAT
    GGTGGATCTA AGAGGAACGC TCTACTGAAG TGGTGCCAGA AGAAAACAGA GGGCTATCCG
    AATATAGACG TGACGAACTT CAGCAGCAGC TGGAGCGACG GCCTAGCGTT CTGCGCTCTC
    TTACACACGT ATCTGCCTGC ACACATCCCC TACCAGGAGC TCATCAGCCA AGACAAGGGG
    CGAAATCTAA CTCTGGCTTT TCAAGCAGCC GAAAGCATTG GTATCAAGCC CTCTCTGGAC
    TTCGAGGAAT TGATGCGCAC CGATCGACCC GACTGGCAAA GCGTCATGCA ATACGTGTCT
    CAGATTTACA AATACTTCGA GACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005168689.1
    CDS303..3089
    Translation

    Target ORF information:

    RefSeq Version XM_005168632.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005168632.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGGAACC AGGAAACCCG GGTGGAAGAT CCGGACACAG GTGTCCAGTC AACCAAAAAG 
    TCAGGGATCC CAGCTCCTCG TGAGATCCCC CAAAGCATCT CCAGAGAACG TCTCTCATCG
    CGCGATCAAC AGAAAAGCTC TACAGCCAAA AAGATGGTCA CCAGTTCCAG CGCCTCAAGC
    ATCTCTGCGC TGGCCAAGCA CTCACGTGGC TCTGTCAAGA CCAAAGCGGA GTCTGCGGCC
    TTGGACAAGA CGTTGCTGGA GAGTCAGGTG AGGGAGCTTC TGGCTGAGGC CAAAACCAAA
    GAGTTCGAGA TCAGTAAGCT ACGAACGGAG CTTCAACGGC ATAAAGGGAA GGATTCGCCA
    TCAGAGGCTG GGACACCAGA AGGGAATTCA GAGATGGAAA GGACACCGGC TCTGACTGGA
    GACGTCCAGG TGCTTGTAGG TGAACTTAGG GAGAAGAACG GACGTTTCCA ACGTGAGTTG
    GCCACTTTGC GGGAAGAGAA TCAATCTTTG AAGCAAAAGC TCATTGCATT GGAGAGCGCC
    AGCTCCAGCA AGCATTCTGT TAACGGAGTT GTCCCAGATA ACATCAATGG AAGCGGTCTT
    AAAACTTCAG TGTCATCTGC AAGTGACATC AAAGTCTCTC CTTTACCCTC TTTAGACTCC
    TCTGAGTTTG AGAAGATCCC CTCACGCTCA GACTCTGTCA GTAGCAGCAT TAAGAGCGTG
    GTGCCCAGCG GTGTGGTGAA AGATCTCTCT GTGGAGTCTC TGACAGAACG CATCCAGAAG
    ATGGAAGAGA GTCACCACAG TACGGCCGAA GAGCTTCAGG CCACTCTACA GGAGTTGGCT
    GACCAGCAAC AGGTCGTCCA GGAGCTGACA GCTGAGAATG AGCGTTTGGC AGAGGAGAAA
    GGCCTCTTGC AGACTTCCCT ACAGCAGCAA AGGGAGCGCG TAGAACTCTT GGCGCAACAA
    AACGAGACTC TTCTCCAGAG GCTCAGAGAA CAAGCTCAGA GTCAAGAAGC TGAGGCTTCA
    CGTGCCTCTC GGATGGCAGA ACTCGAACAG CGTTTGGCTG AGCAAGTAGA GAGTTCGCGA
    TTTGAAAGAG AGAAGCTGGT AGACATCCAG CAGCAGCTGA CAGGGAGTCT CAGAGCCTTG
    GAGAAGGAGA ATCAGGAGGC GCAAACTGCT GTGAAGAGCT TAAGGGAAGA AGAAGGGCTT
    CTGCAAGGGC ATCTGGAGAG TGAGAAGCTT GCTAGAGATG AGGCAGTGAG GAAAACTGAG
    GAACAGAGGC TAGCCATGGA GGCTCTGAGG GTGGACAATG CGAGTATGAA GGCGCAGGTG
    GAGGTGGAGC GGCAGAAGGT TGCTGAGCTG AAGGCAGTGC AGAGCGCTAG TGACAACACT
    GAGCTGCAAA GTCTGCTCAA AGTGGCGCAT GAAGATCGAG ACAAGCTGGA GATGACCTGC
    ACAGAGCTAC GACAAGAGCT GCAGCAGGTG GAGGCAGACA GACAGCAGCT TCTGGAGAAG
    GTGGAGAAAC AGGAACAAGA TCATAAAGCC AACACAAATT CTCTCGAGGA AAAGAGCATA
    GATGCTGAGA ACCAAATCAA GGACCTGAAA GAGACCATCT TCGAGTTGGA GGACCAGGTG
    GAGCAGCAAC GGGCTGTTAG ACTGCACACC AACCAGACCA TCCTGGACCT GGAGAATCAA
    ATCAAGAAGC TGGAAGACAA CAAAACTGAA CTGGAGAAGC AGATTAAGGT CGTGAATAAA
    CAAATGAAGG ATGAAACGGA GGAGTGGCGC CGTTTCCAGG CTGACCTCCA GACAGCAGTA
    GTGGTTGCCA ATGACATAAA GGTGGAGGCT CAGCAGGAGC TGCGGACGCT GCGCAGACGT
    CTGCAGGAGG AGCAGGAGCA CAGCGCGAGA CTGGCTTCAG AGCTGGAGCA GATCCAAGGC
    ACCAGGTCAT GGAATGAAGA TGCCAGCCTC TCCGACTCAG ACGGCAGTCC ACACTGGAGC
    CGAATGACCA TTTCCCAGAA TGCACCTGTG GGTTTCCTAA CCAATGGCAA TGGCAGTGGA
    AGCAGTGTGT CCTCAGAGCC AGAAGCCACG GTCAAATCTC TCATCAAATC ATTTGACACC
    GCAACGCCGA ATGGATCTAG CAGCTCAGTA CAGATACACA CATCTCCCAG AAGCCCCCTG
    AGTGGCATAC CAGTCCGTAC AGCTCCCGCT GCTGCCGTCT CACCCATTCA GAGGCACTCT
    GGAATCAAGC CACTGTCGAA GACCCTCGAG AGAAGAATTG CCTTTGGAGA TTTCCCACAC
    AATGGTTCAT GTGATGAACT GAAGCCCTCG ACTCTTATGA GGAAGAGCCC GTCTCTAGAG
    TCTGTGATTA AAACTCCAGC TCACTTCAAC GGCCGCACTG CATCGTTTAC TTACAACAGA
    GCCAACAGCA AACTCAGTGT GGAAAGAACA GACCCTCTGT CCGCTCTGGC GCGGGAGTAT
    GGTGGATCTA AGAGGAACGC TCTACTGAAG TGGTGCCAGA AGAAAACAGA GGGCTATCCG
    AATATAGACG TGACGAACTT CAGCAGCAGC TGGAGCGACG GCCTAGCGTT CTGCGCTCTC
    TTACACACGT ATCTGCCTGC ACACATCCCC TACCAGGAGC TCATCAGCCA AGACAAGGGG
    CGAAATCTAA CTCTGGCTTT TCAAGCAGCC GAAAGCATTG GTATCAAGCC CTCTCTGGAC
    TTCGAGGAAT TGATGCGCAC CGATCGACCC GACTGGCAAA GCGTCATGCA ATACGTGTCT
    CAGATTTACA AATACTTCGA GACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa11132
    Clone ID Related Accession (Same CDS sequence) XM_005168632.4 , XM_005168632.3
    Accession Version XM_005168632.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2787bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product cytospin-B isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168632.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGGAACC AGGAAACCCG GGTGGAAGAT CCGGACACAG GTGTCCAGTC AACCAAAAAG 
    TCAGGGATCC CAGCTCCTCG TGAGATCCCC CAAAGCATCT CCAGAGAACG TCTCTCATCG
    CGCGATCAAC AGAAAAGCTC TACAGCCAAA AAGATGGTCA CCAGTTCCAG CGCCTCAAGC
    ATCTCTGCGC TGGCCAAGCA CTCACGTGGC TCTGTCAAGA CCAAAGCGGA GTCTGCGGCC
    TTGGACAAGA CGTTGCTGGA GAGTCAGGTG AGGGAGCTTC TGGCTGAGGC CAAAACCAAA
    GAGTTCGAGA TCAGTAAGCT ACGAACGGAG CTTCAACGGC ATAAAGGGAA GGATTCGCCA
    TCAGAGGCTG GGACACCAGA AGGGAATTCA GAGATGGAAA GGACACCGGC TCTGACTGGA
    GACGTCCAGG TGCTTGTAGG TGAACTTAGG GAGAAGAACG GACGTTTCCA ACGTGAGTTG
    GCCACTTTGC GGGAAGAGAA TCAATCTTTG AAGCAAAAGC TCATTGCATT GGAGAGCGCC
    AGCTCCAGCA AGCATTCTGT TAACGGAGTT GTCCCAGATA ACATCAATGG AAGCGGTCTT
    AAAACTTCAG TGTCATCTGC AAGTGACATC AAAGTCTCTC CTTTACCCTC TTTAGACTCC
    TCTGAGTTTG AGAAGATCCC CTCACGCTCA GACTCTGTCA GTAGCAGCAT TAAGAGCGTG
    GTGCCCAGCG GTGTGGTGAA AGATCTCTCT GTGGAGTCTC TGACAGAACG CATCCAGAAG
    ATGGAAGAGA GTCACCACAG TACGGCCGAA GAGCTTCAGG CCACTCTACA GGAGTTGGCT
    GACCAGCAAC AGGTCGTCCA GGAGCTGACA GCTGAGAATG AGCGTTTGGC AGAGGAGAAA
    GGCCTCTTGC AGACTTCCCT ACAGCAGCAA AGGGAGCGCG TAGAACTCTT GGCGCAACAA
    AACGAGACTC TTCTCCAGAG GCTCAGAGAA CAAGCTCAGA GTCAAGAAGC TGAGGCTTCA
    CGTGCCTCTC GGATGGCAGA ACTCGAACAG CGTTTGGCTG AGCAAGTAGA GAGTTCGCGA
    TTTGAAAGAG AGAAGCTGGT AGACATCCAG CAGCAGCTGA CAGGGAGTCT CAGAGCCTTG
    GAGAAGGAGA ATCAGGAGGC GCAAACTGCT GTGAAGAGCT TAAGGGAAGA AGAAGGGCTT
    CTGCAAGGGC ATCTGGAGAG TGAGAAGCTT GCTAGAGATG AGGCAGTGAG GAAAACTGAG
    GAACAGAGGC TAGCCATGGA GGCTCTGAGG GTGGACAATG CGAGTATGAA GGCGCAGGTG
    GAGGTGGAGC GGCAGAAGGT TGCTGAGCTG AAGGCAGTGC AGAGCGCTAG TGACAACACT
    GAGCTGCAAA GTCTGCTCAA AGTGGCGCAT GAAGATCGAG ACAAGCTGGA GATGACCTGC
    ACAGAGCTAC GACAAGAGCT GCAGCAGGTG GAGGCAGACA GACAGCAGCT TCTGGAGAAG
    GTGGAGAAAC AGGAACAAGA TCATAAAGCC AACACAAATT CTCTCGAGGA AAAGAGCATA
    GATGCTGAGA ACCAAATCAA GGACCTGAAA GAGACCATCT TCGAGTTGGA GGACCAGGTG
    GAGCAGCAAC GGGCTGTTAG ACTGCACACC AACCAGACCA TCCTGGACCT GGAGAATCAA
    ATCAAGAAGC TGGAAGACAA CAAAACTGAA CTGGAGAAGC AGATTAAGGT CGTGAATAAA
    CAAATGAAGG ATGAAACGGA GGAGTGGCGC CGTTTCCAGG CTGACCTCCA GACAGCAGTA
    GTGGTTGCCA ATGACATAAA GGTGGAGGCT CAGCAGGAGC TGCGGACGCT GCGCAGACGT
    CTGCAGGAGG AGCAGGAGCA CAGCGCGAGA CTGGCTTCAG AGCTGGAGCA GATCCAAGGC
    ACCAGGTCAT GGAATGAAGA TGCCAGCCTC TCCGACTCAG ACGGCAGTCC ACACTGGAGC
    CGAATGACCA TTTCCCAGAA TGCACCTGTG GGTTTCCTAA CCAATGGCAA TGGCAGTGGA
    AGCAGTGTGT CCTCAGAGCC AGAAGCCACG GTCAAATCTC TCATCAAATC ATTTGACACC
    GCAACGCCGA ATGGATCTAG CAGCTCAGTA CAGATACACA CATCTCCCAG AAGCCCCCTG
    AGTGGCATAC CAGTCCGTAC AGCTCCCGCT GCTGCCGTCT CACCCATTCA GAGGCACTCT
    GGAATCAAGC CACTGTCGAA GACCCTCGAG AGAAGAATTG CCTTTGGAGA TTTCCCACAC
    AATGGTTCAT GTGATGAACT GAAGCCCTCG ACTCTTATGA GGAAGAGCCC GTCTCTAGAG
    TCTGTGATTA AAACTCCAGC TCACTTCAAC GGCCGCACTG CATCGTTTAC TTACAACAGA
    GCCAACAGCA AACTCAGTGT GGAAAGAACA GACCCTCTGT CCGCTCTGGC GCGGGAGTAT
    GGTGGATCTA AGAGGAACGC TCTACTGAAG TGGTGCCAGA AGAAAACAGA GGGCTATCCG
    AATATAGACG TGACGAACTT CAGCAGCAGC TGGAGCGACG GCCTAGCGTT CTGCGCTCTC
    TTACACACGT ATCTGCCTGC ACACATCCCC TACCAGGAGC TCATCAGCCA AGACAAGGGG
    CGAAATCTAA CTCTGGCTTT TCAAGCAGCC GAAAGCATTG GTATCAAGCC CTCTCTGGAC
    TTCGAGGAAT TGATGCGCAC CGATCGACCC GACTGGCAAA GCGTCATGCA ATACGTGTCT
    CAGATTTACA AATACTTCGA GACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005168689.1
    CDS301..3087
    Translation

    Target ORF information:

    RefSeq Version XM_005168632.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005168632.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGGAACC AGGAAACCCG GGTGGAAGAT CCGGACACAG GTGTCCAGTC AACCAAAAAG 
    TCAGGGATCC CAGCTCCTCG TGAGATCCCC CAAAGCATCT CCAGAGAACG TCTCTCATCG
    CGCGATCAAC AGAAAAGCTC TACAGCCAAA AAGATGGTCA CCAGTTCCAG CGCCTCAAGC
    ATCTCTGCGC TGGCCAAGCA CTCACGTGGC TCTGTCAAGA CCAAAGCGGA GTCTGCGGCC
    TTGGACAAGA CGTTGCTGGA GAGTCAGGTG AGGGAGCTTC TGGCTGAGGC CAAAACCAAA
    GAGTTCGAGA TCAGTAAGCT ACGAACGGAG CTTCAACGGC ATAAAGGGAA GGATTCGCCA
    TCAGAGGCTG GGACACCAGA AGGGAATTCA GAGATGGAAA GGACACCGGC TCTGACTGGA
    GACGTCCAGG TGCTTGTAGG TGAACTTAGG GAGAAGAACG GACGTTTCCA ACGTGAGTTG
    GCCACTTTGC GGGAAGAGAA TCAATCTTTG AAGCAAAAGC TCATTGCATT GGAGAGCGCC
    AGCTCCAGCA AGCATTCTGT TAACGGAGTT GTCCCAGATA ACATCAATGG AAGCGGTCTT
    AAAACTTCAG TGTCATCTGC AAGTGACATC AAAGTCTCTC CTTTACCCTC TTTAGACTCC
    TCTGAGTTTG AGAAGATCCC CTCACGCTCA GACTCTGTCA GTAGCAGCAT TAAGAGCGTG
    GTGCCCAGCG GTGTGGTGAA AGATCTCTCT GTGGAGTCTC TGACAGAACG CATCCAGAAG
    ATGGAAGAGA GTCACCACAG TACGGCCGAA GAGCTTCAGG CCACTCTACA GGAGTTGGCT
    GACCAGCAAC AGGTCGTCCA GGAGCTGACA GCTGAGAATG AGCGTTTGGC AGAGGAGAAA
    GGCCTCTTGC AGACTTCCCT ACAGCAGCAA AGGGAGCGCG TAGAACTCTT GGCGCAACAA
    AACGAGACTC TTCTCCAGAG GCTCAGAGAA CAAGCTCAGA GTCAAGAAGC TGAGGCTTCA
    CGTGCCTCTC GGATGGCAGA ACTCGAACAG CGTTTGGCTG AGCAAGTAGA GAGTTCGCGA
    TTTGAAAGAG AGAAGCTGGT AGACATCCAG CAGCAGCTGA CAGGGAGTCT CAGAGCCTTG
    GAGAAGGAGA ATCAGGAGGC GCAAACTGCT GTGAAGAGCT TAAGGGAAGA AGAAGGGCTT
    CTGCAAGGGC ATCTGGAGAG TGAGAAGCTT GCTAGAGATG AGGCAGTGAG GAAAACTGAG
    GAACAGAGGC TAGCCATGGA GGCTCTGAGG GTGGACAATG CGAGTATGAA GGCGCAGGTG
    GAGGTGGAGC GGCAGAAGGT TGCTGAGCTG AAGGCAGTGC AGAGCGCTAG TGACAACACT
    GAGCTGCAAA GTCTGCTCAA AGTGGCGCAT GAAGATCGAG ACAAGCTGGA GATGACCTGC
    ACAGAGCTAC GACAAGAGCT GCAGCAGGTG GAGGCAGACA GACAGCAGCT TCTGGAGAAG
    GTGGAGAAAC AGGAACAAGA TCATAAAGCC AACACAAATT CTCTCGAGGA AAAGAGCATA
    GATGCTGAGA ACCAAATCAA GGACCTGAAA GAGACCATCT TCGAGTTGGA GGACCAGGTG
    GAGCAGCAAC GGGCTGTTAG ACTGCACACC AACCAGACCA TCCTGGACCT GGAGAATCAA
    ATCAAGAAGC TGGAAGACAA CAAAACTGAA CTGGAGAAGC AGATTAAGGT CGTGAATAAA
    CAAATGAAGG ATGAAACGGA GGAGTGGCGC CGTTTCCAGG CTGACCTCCA GACAGCAGTA
    GTGGTTGCCA ATGACATAAA GGTGGAGGCT CAGCAGGAGC TGCGGACGCT GCGCAGACGT
    CTGCAGGAGG AGCAGGAGCA CAGCGCGAGA CTGGCTTCAG AGCTGGAGCA GATCCAAGGC
    ACCAGGTCAT GGAATGAAGA TGCCAGCCTC TCCGACTCAG ACGGCAGTCC ACACTGGAGC
    CGAATGACCA TTTCCCAGAA TGCACCTGTG GGTTTCCTAA CCAATGGCAA TGGCAGTGGA
    AGCAGTGTGT CCTCAGAGCC AGAAGCCACG GTCAAATCTC TCATCAAATC ATTTGACACC
    GCAACGCCGA ATGGATCTAG CAGCTCAGTA CAGATACACA CATCTCCCAG AAGCCCCCTG
    AGTGGCATAC CAGTCCGTAC AGCTCCCGCT GCTGCCGTCT CACCCATTCA GAGGCACTCT
    GGAATCAAGC CACTGTCGAA GACCCTCGAG AGAAGAATTG CCTTTGGAGA TTTCCCACAC
    AATGGTTCAT GTGATGAACT GAAGCCCTCG ACTCTTATGA GGAAGAGCCC GTCTCTAGAG
    TCTGTGATTA AAACTCCAGC TCACTTCAAC GGCCGCACTG CATCGTTTAC TTACAACAGA
    GCCAACAGCA AACTCAGTGT GGAAAGAACA GACCCTCTGT CCGCTCTGGC GCGGGAGTAT
    GGTGGATCTA AGAGGAACGC TCTACTGAAG TGGTGCCAGA AGAAAACAGA GGGCTATCCG
    AATATAGACG TGACGAACTT CAGCAGCAGC TGGAGCGACG GCCTAGCGTT CTGCGCTCTC
    TTACACACGT ATCTGCCTGC ACACATCCCC TACCAGGAGC TCATCAGCCA AGACAAGGGG
    CGAAATCTAA CTCTGGCTTT TCAAGCAGCC GAAAGCATTG GTATCAAGCC CTCTCTGGAC
    TTCGAGGAAT TGATGCGCAC CGATCGACCC GACTGGCAAA GCGTCATGCA ATACGTGTCT
    CAGATTTACA AATACTTCGA GACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa11133
    Clone ID Related Accession (Same CDS sequence) XM_005168633.3 , XM_005168633.4
    Accession Version XM_005168633.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2229bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product cytospin-B isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168633.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTTAAAAG GGGGCACCGC CAAGGTGGCG CTGCCCAGGA TGGGAGCCCC AGAACGTGCA 
    AAGCTGTCCA CCATCAGTTC ACAAAGCACA TACTCTGGCA ACATCGGGAT GAAGACCTCC
    AGGTCGTCTA GCACCATCGC CTCTGACCTG CGACTCAGCA AGATAAAGAG GGCGAGCAGT
    GATGATGCTC TGGCTAAACC TGTCCTGGGT TCAGTGTCCC GCATAAAGAA GACCATCACC
    ACAGGAGCCA TCGCTGAGCT TACAGACAAC CGGCCACGCA GCGCATCAAG TGTCCAGTCA
    ACCAAAAAGT CAGGGATCCC AGCTCCTCGT GAGATCCCCC AAAGCATCTC CAGAGAACGT
    CTCTCATCGC GCGATCAACA GAAAAGCTCT ACAGCCAAAA AGATGGTCAC CAGTTCCAGC
    GCCTCAAGCA TCTCTGCGCT GGCCAAGCAC TCACGTGGCT CTGTCAAGAC CAAAGCGGAG
    TCTGCGGCCT TGGACAAGAC GTTGCTGGAG AGTCAGGTGA GGGAGCTTCT GGCTGAGGCC
    AAAACCAAAG AGTTCGAGAT CAGTAAGCTA CGAACGGAGC TTCAACGGCA TAAAGGGAAG
    GATTCGCCAT CAGAGGCTGG GACACCAGAA GGGAATTCAG AGATGGAAAG GACACCGGCT
    CTGACTGGAG ACGTCCAGGT GCTTGTAGGT GAACTTAGGG AGAAGAACGG ACGTTTCCAA
    CGTGAGTTGG CCACTTTGCG GGAAGAGAAT CAATCTTTGA AGCAAAAGCT CATTGCATTG
    GAGAGCGCCA GCTCCAGCAA GCATTCTGTT AACGGAGTTG TCCCAGATAA CATCAATGGA
    AGCGGTCTTA AAACTTCAGT GTCATCTGCA AGTGACATCA AAGTCTCTCC TTTACCCTCT
    TTAGACTCCT CTGAGTTTGA GAAGATCCCC TCACGCTCAG ACTCTGTCAG TAGCAGCATT
    AAGAGCGTGG TGCCCAGCGG TGTGGTGAAA GATCTCTCTG TGGAGTCTCT GACAGAACGC
    ATCCAGAAGA TGGAAGAGAG TCACCACAGT ACGGCCGAAG AGCTTCAGGC CACTCTACAG
    GAGTTGGCTG ACCAGCAACA GGTCGTCCAG GAGCTGACAG CTGAGAATGA GCGTTTGGCA
    GAGGAGAAAG GCCTCTTGCA GACTTCCCTA CAGCAGCAAA GGGAGCGCGT AGAACTCTTG
    GCGCAACAAA ACGAGACTCT TCTCCAGAGG CTCAGAGAAC AAGCTCAGAG TCAAGAAGCT
    GAGGCTTCAC GTGCCTCTCG GATGGCAGAA CTCGAACAGC GTTTGGCTGA GCAAGTAGAG
    AGTTCGCGAT TTGAAAGAGA GAAGCTGGTA GACATCCAGC AGCAGCTGAC AGGGAGTCTC
    AGAGCCTTGG AGAAGGAGAA TCAGGAGGCG CAAACTGCTG TGAAGAGCTT AAGGGAAGAA
    GAAGGGCTTC TGCAAGGGCA TCTGGAGAGT GAGAAGCTTG CTAGAGATGA GGCAGTGAGG
    AAAACTGAGG AACAGAGGCT AGCCATGGAG GCTCTGAGGG TGGACAATGC GAGTATGAAG
    GCGCAGGTGG AGGTGGAGCG GCAGAAGGTT GCTGAGCTGA AGGCAGTGCA GAGCGCTAGT
    GACAACACTG AGCTGCAAAG TCTGCTCAAA GTGGCGCATG AAGATCGAGA CAAGCTGGAG
    ATGACCTGCA CAGAGCTACG ACAAGAGCTG CAGCAGGTGG AGGCAGACAG ACAGCAGCTT
    CTGGAGAAGG TGGAGAAACA GGAACAAGAT CATAAAGCCA ACACAAATTC TCTCGAGGAA
    AAGAGCATAG ATGCTGAGAA CCAAATCAAG GACCTGAAAG AGACCATCTT CGAGTTGGAG
    GACCAGGTGG AGCAGCAACG GGCTGTTAGA CTGCACACCA ACCAGACCAT CCTGGACCTG
    GAGAATCAAA TCAAGAAGCT GGAAGACAAC AAAACTGAAC TGGAGAAGCA GATTAAGGTC
    GTGAATAAAC AAATGAAGGA TGAAACGGAG GAGTGGCGCC GTTTCCAGGC TGACCTCCAG
    ACAGCAGTAG TGGTTGCCAA TGACATAAAG GTGGAGGCTC AGCAGGAGCT GCGGACGCTG
    CGCAGACGTC TGCAGGAGGA GCAGGAGCAC AGCGCGAGAC TGGCTTCAGA GCTGGAGCAG
    ATCCAAGGCA CCAGAATCGC CAGTGGAGCG GCGTTTGAGA CCTCCAAGAC TGCAGTTGAG
    GGGCGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005168690.1
    CDS105..2333
    Translation

    Target ORF information:

    RefSeq Version XM_005168633.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005168633.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTTAAAAG GGGGCACCGC CAAGGTGGCG CTGCCCAGGA TGGGAGCCCC AGAACGTGCA 
    AAGCTGTCCA CCATCAGTTC ACAAAGCACA TACTCTGGCA ACATCGGGAT GAAGACCTCC
    AGGTCGTCTA GCACCATCGC CTCTGACCTG CGACTCAGCA AGATAAAGAG GGCGAGCAGT
    GATGATGCTC TGGCTAAACC TGTCCTGGGT TCAGTGTCCC GCATAAAGAA GACCATCACC
    ACAGGAGCCA TCGCTGAGCT TACAGACAAC CGGCCACGCA GCGCATCAAG TGTCCAGTCA
    ACCAAAAAGT CAGGGATCCC AGCTCCTCGT GAGATCCCCC AAAGCATCTC CAGAGAACGT
    CTCTCATCGC GCGATCAACA GAAAAGCTCT ACAGCCAAAA AGATGGTCAC CAGTTCCAGC
    GCCTCAAGCA TCTCTGCGCT GGCCAAGCAC TCACGTGGCT CTGTCAAGAC CAAAGCGGAG
    TCTGCGGCCT TGGACAAGAC GTTGCTGGAG AGTCAGGTGA GGGAGCTTCT GGCTGAGGCC
    AAAACCAAAG AGTTCGAGAT CAGTAAGCTA CGAACGGAGC TTCAACGGCA TAAAGGGAAG
    GATTCGCCAT CAGAGGCTGG GACACCAGAA GGGAATTCAG AGATGGAAAG GACACCGGCT
    CTGACTGGAG ACGTCCAGGT GCTTGTAGGT GAACTTAGGG AGAAGAACGG ACGTTTCCAA
    CGTGAGTTGG CCACTTTGCG GGAAGAGAAT CAATCTTTGA AGCAAAAGCT CATTGCATTG
    GAGAGCGCCA GCTCCAGCAA GCATTCTGTT AACGGAGTTG TCCCAGATAA CATCAATGGA
    AGCGGTCTTA AAACTTCAGT GTCATCTGCA AGTGACATCA AAGTCTCTCC TTTACCCTCT
    TTAGACTCCT CTGAGTTTGA GAAGATCCCC TCACGCTCAG ACTCTGTCAG TAGCAGCATT
    AAGAGCGTGG TGCCCAGCGG TGTGGTGAAA GATCTCTCTG TGGAGTCTCT GACAGAACGC
    ATCCAGAAGA TGGAAGAGAG TCACCACAGT ACGGCCGAAG AGCTTCAGGC CACTCTACAG
    GAGTTGGCTG ACCAGCAACA GGTCGTCCAG GAGCTGACAG CTGAGAATGA GCGTTTGGCA
    GAGGAGAAAG GCCTCTTGCA GACTTCCCTA CAGCAGCAAA GGGAGCGCGT AGAACTCTTG
    GCGCAACAAA ACGAGACTCT TCTCCAGAGG CTCAGAGAAC AAGCTCAGAG TCAAGAAGCT
    GAGGCTTCAC GTGCCTCTCG GATGGCAGAA CTCGAACAGC GTTTGGCTGA GCAAGTAGAG
    AGTTCGCGAT TTGAAAGAGA GAAGCTGGTA GACATCCAGC AGCAGCTGAC AGGGAGTCTC
    AGAGCCTTGG AGAAGGAGAA TCAGGAGGCG CAAACTGCTG TGAAGAGCTT AAGGGAAGAA
    GAAGGGCTTC TGCAAGGGCA TCTGGAGAGT GAGAAGCTTG CTAGAGATGA GGCAGTGAGG
    AAAACTGAGG AACAGAGGCT AGCCATGGAG GCTCTGAGGG TGGACAATGC GAGTATGAAG
    GCGCAGGTGG AGGTGGAGCG GCAGAAGGTT GCTGAGCTGA AGGCAGTGCA GAGCGCTAGT
    GACAACACTG AGCTGCAAAG TCTGCTCAAA GTGGCGCATG AAGATCGAGA CAAGCTGGAG
    ATGACCTGCA CAGAGCTACG ACAAGAGCTG CAGCAGGTGG AGGCAGACAG ACAGCAGCTT
    CTGGAGAAGG TGGAGAAACA GGAACAAGAT CATAAAGCCA ACACAAATTC TCTCGAGGAA
    AAGAGCATAG ATGCTGAGAA CCAAATCAAG GACCTGAAAG AGACCATCTT CGAGTTGGAG
    GACCAGGTGG AGCAGCAACG GGCTGTTAGA CTGCACACCA ACCAGACCAT CCTGGACCTG
    GAGAATCAAA TCAAGAAGCT GGAAGACAAC AAAACTGAAC TGGAGAAGCA GATTAAGGTC
    GTGAATAAAC AAATGAAGGA TGAAACGGAG GAGTGGCGCC GTTTCCAGGC TGACCTCCAG
    ACAGCAGTAG TGGTTGCCAA TGACATAAAG GTGGAGGCTC AGCAGGAGCT GCGGACGCTG
    CGCAGACGTC TGCAGGAGGA GCAGGAGCAC AGCGCGAGAC TGGCTTCAGA GCTGGAGCAG
    ATCCAAGGCA CCAGAATCGC CAGTGGAGCG GCGTTTGAGA CCTCCAAGAC TGCAGTTGAG
    GGGCGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa11133
    Clone ID Related Accession (Same CDS sequence) XM_005168633.3 , XM_005168633.4
    Accession Version XM_005168633.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2229bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product cytospin-B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168633.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTTAAAAG GGGGCACCGC CAAGGTGGCG CTGCCCAGGA TGGGAGCCCC AGAACGTGCA 
    AAGCTGTCCA CCATCAGTTC ACAAAGCACA TACTCTGGCA ACATCGGGAT GAAGACCTCC
    AGGTCGTCTA GCACCATCGC CTCTGACCTG CGACTCAGCA AGATAAAGAG GGCGAGCAGT
    GATGATGCTC TGGCTAAACC TGTCCTGGGT TCAGTGTCCC GCATAAAGAA GACCATCACC
    ACAGGAGCCA TCGCTGAGCT TACAGACAAC CGGCCACGCA GCGCATCAAG TGTCCAGTCA
    ACCAAAAAGT CAGGGATCCC AGCTCCTCGT GAGATCCCCC AAAGCATCTC CAGAGAACGT
    CTCTCATCGC GCGATCAACA GAAAAGCTCT ACAGCCAAAA AGATGGTCAC CAGTTCCAGC
    GCCTCAAGCA TCTCTGCGCT GGCCAAGCAC TCACGTGGCT CTGTCAAGAC CAAAGCGGAG
    TCTGCGGCCT TGGACAAGAC GTTGCTGGAG AGTCAGGTGA GGGAGCTTCT GGCTGAGGCC
    AAAACCAAAG AGTTCGAGAT CAGTAAGCTA CGAACGGAGC TTCAACGGCA TAAAGGGAAG
    GATTCGCCAT CAGAGGCTGG GACACCAGAA GGGAATTCAG AGATGGAAAG GACACCGGCT
    CTGACTGGAG ACGTCCAGGT GCTTGTAGGT GAACTTAGGG AGAAGAACGG ACGTTTCCAA
    CGTGAGTTGG CCACTTTGCG GGAAGAGAAT CAATCTTTGA AGCAAAAGCT CATTGCATTG
    GAGAGCGCCA GCTCCAGCAA GCATTCTGTT AACGGAGTTG TCCCAGATAA CATCAATGGA
    AGCGGTCTTA AAACTTCAGT GTCATCTGCA AGTGACATCA AAGTCTCTCC TTTACCCTCT
    TTAGACTCCT CTGAGTTTGA GAAGATCCCC TCACGCTCAG ACTCTGTCAG TAGCAGCATT
    AAGAGCGTGG TGCCCAGCGG TGTGGTGAAA GATCTCTCTG TGGAGTCTCT GACAGAACGC
    ATCCAGAAGA TGGAAGAGAG TCACCACAGT ACGGCCGAAG AGCTTCAGGC CACTCTACAG
    GAGTTGGCTG ACCAGCAACA GGTCGTCCAG GAGCTGACAG CTGAGAATGA GCGTTTGGCA
    GAGGAGAAAG GCCTCTTGCA GACTTCCCTA CAGCAGCAAA GGGAGCGCGT AGAACTCTTG
    GCGCAACAAA ACGAGACTCT TCTCCAGAGG CTCAGAGAAC AAGCTCAGAG TCAAGAAGCT
    GAGGCTTCAC GTGCCTCTCG GATGGCAGAA CTCGAACAGC GTTTGGCTGA GCAAGTAGAG
    AGTTCGCGAT TTGAAAGAGA GAAGCTGGTA GACATCCAGC AGCAGCTGAC AGGGAGTCTC
    AGAGCCTTGG AGAAGGAGAA TCAGGAGGCG CAAACTGCTG TGAAGAGCTT AAGGGAAGAA
    GAAGGGCTTC TGCAAGGGCA TCTGGAGAGT GAGAAGCTTG CTAGAGATGA GGCAGTGAGG
    AAAACTGAGG AACAGAGGCT AGCCATGGAG GCTCTGAGGG TGGACAATGC GAGTATGAAG
    GCGCAGGTGG AGGTGGAGCG GCAGAAGGTT GCTGAGCTGA AGGCAGTGCA GAGCGCTAGT
    GACAACACTG AGCTGCAAAG TCTGCTCAAA GTGGCGCATG AAGATCGAGA CAAGCTGGAG
    ATGACCTGCA CAGAGCTACG ACAAGAGCTG CAGCAGGTGG AGGCAGACAG ACAGCAGCTT
    CTGGAGAAGG TGGAGAAACA GGAACAAGAT CATAAAGCCA ACACAAATTC TCTCGAGGAA
    AAGAGCATAG ATGCTGAGAA CCAAATCAAG GACCTGAAAG AGACCATCTT CGAGTTGGAG
    GACCAGGTGG AGCAGCAACG GGCTGTTAGA CTGCACACCA ACCAGACCAT CCTGGACCTG
    GAGAATCAAA TCAAGAAGCT GGAAGACAAC AAAACTGAAC TGGAGAAGCA GATTAAGGTC
    GTGAATAAAC AAATGAAGGA TGAAACGGAG GAGTGGCGCC GTTTCCAGGC TGACCTCCAG
    ACAGCAGTAG TGGTTGCCAA TGACATAAAG GTGGAGGCTC AGCAGGAGCT GCGGACGCTG
    CGCAGACGTC TGCAGGAGGA GCAGGAGCAC AGCGCGAGAC TGGCTTCAGA GCTGGAGCAG
    ATCCAAGGCA CCAGAATCGC CAGTGGAGCG GCGTTTGAGA CCTCCAAGAC TGCAGTTGAG
    GGGCGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005168690.1
    CDS106..2334
    Translation

    Target ORF information:

    RefSeq Version XM_005168633.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005168633.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTTAAAAG GGGGCACCGC CAAGGTGGCG CTGCCCAGGA TGGGAGCCCC AGAACGTGCA 
    AAGCTGTCCA CCATCAGTTC ACAAAGCACA TACTCTGGCA ACATCGGGAT GAAGACCTCC
    AGGTCGTCTA GCACCATCGC CTCTGACCTG CGACTCAGCA AGATAAAGAG GGCGAGCAGT
    GATGATGCTC TGGCTAAACC TGTCCTGGGT TCAGTGTCCC GCATAAAGAA GACCATCACC
    ACAGGAGCCA TCGCTGAGCT TACAGACAAC CGGCCACGCA GCGCATCAAG TGTCCAGTCA
    ACCAAAAAGT CAGGGATCCC AGCTCCTCGT GAGATCCCCC AAAGCATCTC CAGAGAACGT
    CTCTCATCGC GCGATCAACA GAAAAGCTCT ACAGCCAAAA AGATGGTCAC CAGTTCCAGC
    GCCTCAAGCA TCTCTGCGCT GGCCAAGCAC TCACGTGGCT CTGTCAAGAC CAAAGCGGAG
    TCTGCGGCCT TGGACAAGAC GTTGCTGGAG AGTCAGGTGA GGGAGCTTCT GGCTGAGGCC
    AAAACCAAAG AGTTCGAGAT CAGTAAGCTA CGAACGGAGC TTCAACGGCA TAAAGGGAAG
    GATTCGCCAT CAGAGGCTGG GACACCAGAA GGGAATTCAG AGATGGAAAG GACACCGGCT
    CTGACTGGAG ACGTCCAGGT GCTTGTAGGT GAACTTAGGG AGAAGAACGG ACGTTTCCAA
    CGTGAGTTGG CCACTTTGCG GGAAGAGAAT CAATCTTTGA AGCAAAAGCT CATTGCATTG
    GAGAGCGCCA GCTCCAGCAA GCATTCTGTT AACGGAGTTG TCCCAGATAA CATCAATGGA
    AGCGGTCTTA AAACTTCAGT GTCATCTGCA AGTGACATCA AAGTCTCTCC TTTACCCTCT
    TTAGACTCCT CTGAGTTTGA GAAGATCCCC TCACGCTCAG ACTCTGTCAG TAGCAGCATT
    AAGAGCGTGG TGCCCAGCGG TGTGGTGAAA GATCTCTCTG TGGAGTCTCT GACAGAACGC
    ATCCAGAAGA TGGAAGAGAG TCACCACAGT ACGGCCGAAG AGCTTCAGGC CACTCTACAG
    GAGTTGGCTG ACCAGCAACA GGTCGTCCAG GAGCTGACAG CTGAGAATGA GCGTTTGGCA
    GAGGAGAAAG GCCTCTTGCA GACTTCCCTA CAGCAGCAAA GGGAGCGCGT AGAACTCTTG
    GCGCAACAAA ACGAGACTCT TCTCCAGAGG CTCAGAGAAC AAGCTCAGAG TCAAGAAGCT
    GAGGCTTCAC GTGCCTCTCG GATGGCAGAA CTCGAACAGC GTTTGGCTGA GCAAGTAGAG
    AGTTCGCGAT TTGAAAGAGA GAAGCTGGTA GACATCCAGC AGCAGCTGAC AGGGAGTCTC
    AGAGCCTTGG AGAAGGAGAA TCAGGAGGCG CAAACTGCTG TGAAGAGCTT AAGGGAAGAA
    GAAGGGCTTC TGCAAGGGCA TCTGGAGAGT GAGAAGCTTG CTAGAGATGA GGCAGTGAGG
    AAAACTGAGG AACAGAGGCT AGCCATGGAG GCTCTGAGGG TGGACAATGC GAGTATGAAG
    GCGCAGGTGG AGGTGGAGCG GCAGAAGGTT GCTGAGCTGA AGGCAGTGCA GAGCGCTAGT
    GACAACACTG AGCTGCAAAG TCTGCTCAAA GTGGCGCATG AAGATCGAGA CAAGCTGGAG
    ATGACCTGCA CAGAGCTACG ACAAGAGCTG CAGCAGGTGG AGGCAGACAG ACAGCAGCTT
    CTGGAGAAGG TGGAGAAACA GGAACAAGAT CATAAAGCCA ACACAAATTC TCTCGAGGAA
    AAGAGCATAG ATGCTGAGAA CCAAATCAAG GACCTGAAAG AGACCATCTT CGAGTTGGAG
    GACCAGGTGG AGCAGCAACG GGCTGTTAGA CTGCACACCA ACCAGACCAT CCTGGACCTG
    GAGAATCAAA TCAAGAAGCT GGAAGACAAC AAAACTGAAC TGGAGAAGCA GATTAAGGTC
    GTGAATAAAC AAATGAAGGA TGAAACGGAG GAGTGGCGCC GTTTCCAGGC TGACCTCCAG
    ACAGCAGTAG TGGTTGCCAA TGACATAAAG GTGGAGGCTC AGCAGGAGCT GCGGACGCTG
    CGCAGACGTC TGCAGGAGGA GCAGGAGCAC AGCGCGAGAC TGGCTTCAGA GCTGGAGCAG
    ATCCAAGGCA CCAGAATCGC CAGTGGAGCG GCGTTTGAGA CCTCCAAGAC TGCAGTTGAG
    GGGCGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa11131
    Clone ID Related Accession (Same CDS sequence) XM_009301917.2 , XM_009301918.2 , XM_009301917.3 , XM_685735.8 , XM_685735.7
    Accession Version XM_009301917.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product cytospin-B isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301917.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTAAAAG GGGGCACCGC CAAGGTGGCG CTGCCCAGGA TGGGAGCCCC AGAACGTGCA 
    AAGCTGTCCA CCATCAGTTC ACAAAGCACA TACTCTGGCA ACATCGGGAT GAAGACCTCC
    AGGTCGTCTA GCACCATCGC CTCTGACCTG CGACTCAGCA AGATAAAGAG GGCGAGCAGT
    GATGATGCTC TGGCTAAACC TGTCCTGGGT TCAGTGTCCC GCATAAAGAA GACCATCACC
    ACAGGAGCCA TCGCTGAGCT TACAGACAAC CGGCCACGCA GCGCATCAAG TGTCCAGTCA
    ACCAAAAAGT CAGGGATCCC AGCTCCTCGT GAGATCCCCC AAAGCATCTC CAGAGAACGT
    CTCTCATCGC GCGATCAACA GAAAAGCTCT ACAGCCAAAA AGATGGTCAC CAGTTCCAGC
    GCCTCAAGCA TCTCTGCGCT GGCCAAGCAC TCACGTGGCT CTGTCAAGAC CAAAGCGGAG
    TCTGCGGCCT TGGACAAGAC GTTGCTGGAG AGTCAGGTGA GGGAGCTTCT GGCTGAGGCC
    AAAACCAAAG AGTTCGAGAT CAGTAAGCTA CGAACGGAGC TTCAACGGCA TAAAGGGAAG
    GATTCGCCAT CAGAGGCTGG GACACCAGAA GGGAATTCAG AGATGGAAAG GACACCGGCT
    CTGACTGGAG ACGTCCAGGT GCTTGTAGGT GAACTTAGGG AGAAGAACGG ACGTTTCCAA
    CGTGAGTTGG CCACTTTGCG GGAAGAGAAT CAATCTTTGA AGCAAAAGCT CATTGCATTG
    GAGAGCGCCA GCTCCAGCAA GCATTCTGTT AACGGAGTTG TCCCAGATAA CATCAATGGA
    AGCGGTCTTA AAACTTCAGT GTCATCTGCA AGTGACATCA AAGTCTCTCC TTTACCCTCT
    TTAGACTCCT CTGAGTTTGA GAAGATCCCC TCACGCTCAG ACTCTGTCAG TAGCAGCATT
    AAGAGCGTGG TGCCCAGCGG TGTGGTGAAA GATCTCTCTG TGGAGTCTCT GACAGAACGC
    ATCCAGAAGA TGGAAGAGAG TCACCACAGT ACGGCCGAAG AGCTTCAGGC CACTCTACAG
    GAGTTGGCTG ACCAGCAACA GGTCGTCCAG GAGCTGACAG CTGAGAATGA GCGTTTGGCA
    GAGGAGAAAG GCCTCTTGCA GACTTCCCTA CAGCAGCAAA GGGAGCGCGT AGAACTCTTG
    GCGCAACAAA ACGAGACTCT TCTCCAGAGG CTCAGAGAAC AAGCTCAGAG TCAAGAAGCT
    GAGGCTTCAC GTGCCTCTCG GATGGCAGAA CTCGAACAGC GTTTGGCTGA GCAAGTAGAG
    AGTTCGCGAT TTGAAAGAGA GAAGCTGGTA GACATCCAGC AGCAGCTGAC AGGGAGTCTC
    AGAGCCTTGG AGAAGGAGAA TCAGGAGGCG CAAACTGCTG TGAAGAGCTT AAGGGAAGAA
    GAAGGGCTTC TGCAAGGGCA TCTGGAGAGT GAGAAGCTTG CTAGAGATGA GGCAGTGAGG
    AAAACTGAGG AACAGAGGCT AGCCATGGAG GCTCTGAGGG TGGACAATGC GAGTATGAAG
    GCGCAGGTGG AGGTGGAGCG GCAGAAGGTT GCTGAGCTGA AGGCAGTGCA GAGCGCTAGT
    GACAACACTG AGCTGCAAAG TCTGCTCAAA GTGGCGCATG AAGATCGAGA CAAGCTGGAG
    ATGACCTGCA CAGAGCTACG ACAAGAGCTG CAGCAGGTGG AGGCAGACAG ACAGCAGCTT
    CTGGAGAAGG TGGAGAAACA GGAACAAGAT CATAAAGCCA ACACAAATTC TCTCGAGGAA
    AAGAGCATAG ATGCTGAGAA CCAAATCAAG GACCTGAAAG AGACCATCTT CGAGTTGGAG
    GACCAGGTGG AGCAGCAACG GGCTGTTAGA CTGCACACCA ACCAGACCAT CCTGGACCTG
    GAGAATCAAA TCAAGAAGCT GGAAGACAAC AAAACTGAAC TGGAGAAGCA GATTAAGGTC
    GTGAATAAAC AAATGAAGGA TGAAACGGAG GAGTGGCGCC GTTTCCAGGC TGACCTCCAG
    ACAGCAGTAG TGGTTGCCAA TGACATAAAG GTGGAGGCTC AGCAGGAGCT GCGGACGCTG
    CGCAGACGTC TGCAGGAGGA GCAGGAGCAC AGCGCGAGAC TGGCTTCAGA GCTGGAGCAG
    ATCCAAGGCA CCAGGTCATG GAATGAAGAT GCCAGCCTCT CCGACTCAGA CGGCAGTCCA
    CACTGGAGCC GAATGACCAT TTCCCAGAAT GCACCTGTGG GTTTCCTAAC CAATGGCAAT
    GGCAGTGGAA GCAGTGTGTC CTCAGAGCCA GAAGCCACGG TCAAATCTCT CATCAAATCA
    TTTGACACCG CAACGCCGAA TGGATCTAGC AGCTCAGTAC AGATACACAC ATCTCCCAGA
    AGCCCCCTGA GTGGCATACC AGTCCGTACA GCTCCCGCTG CTGCCGTCTC ACCCATTCAG
    AGGCACTCTG GAATCAAGCC ACTGTCGAAG ACCCTCGAGA GAAGAATTGC CTTTGGAGAT
    TTCCCACACA ATGGTTCATG TGATGAACTG AAGCCCTCGA CTCTTATGAG GAAGAGCCCG
    TCTCTAGAGT CTGTGATTAA AACTCCAGCT CACTTCAACG GCCGCACTGC ATCGTTTACT
    TACAACAGAG CCAACAGCAA ACTCAGTGTG GAAAGAACAG ACCCTCTGTC CGCTCTGGCG
    CGGGAGTATG GTGGATCTAA GAGGAACGCT CTACTGAAGT GGTGCCAGAA GAAAACAGAG
    GGCTATCCGA ATATAGACGT GACGAACTTC AGCAGCAGCT GGAGCGACGG CCTAGCGTTC
    TGCGCTCTCT TACACACGTA TCTGCCTGCA CACATCCCCT ACCAGGAGCT CATCAGCCAA
    GACAAGGGGC GAAATCTAAC TCTGGCTTTT CAAGCAGCCG AAAGCATTGG TATCAAGCCC
    TCTCTGGACT TCGAGGAATT GATGCGCACC GATCGACCCG ACTGGCAAAG CGTCATGCAA
    TACGTGTCTC AGATTTACAA ATACTTCGAG ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300192.1
    CDS186..3221
    Translation

    Target ORF information:

    RefSeq Version XM_009301917.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301917.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTAAAAG GGGGCACCGC CAAGGTGGCG CTGCCCAGGA TGGGAGCCCC AGAACGTGCA 
    AAGCTGTCCA CCATCAGTTC ACAAAGCACA TACTCTGGCA ACATCGGGAT GAAGACCTCC
    AGGTCGTCTA GCACCATCGC CTCTGACCTG CGACTCAGCA AGATAAAGAG GGCGAGCAGT
    GATGATGCTC TGGCTAAACC TGTCCTGGGT TCAGTGTCCC GCATAAAGAA GACCATCACC
    ACAGGAGCCA TCGCTGAGCT TACAGACAAC CGGCCACGCA GCGCATCAAG TGTCCAGTCA
    ACCAAAAAGT CAGGGATCCC AGCTCCTCGT GAGATCCCCC AAAGCATCTC CAGAGAACGT
    CTCTCATCGC GCGATCAACA GAAAAGCTCT ACAGCCAAAA AGATGGTCAC CAGTTCCAGC
    GCCTCAAGCA TCTCTGCGCT GGCCAAGCAC TCACGTGGCT CTGTCAAGAC CAAAGCGGAG
    TCTGCGGCCT TGGACAAGAC GTTGCTGGAG AGTCAGGTGA GGGAGCTTCT GGCTGAGGCC
    AAAACCAAAG AGTTCGAGAT CAGTAAGCTA CGAACGGAGC TTCAACGGCA TAAAGGGAAG
    GATTCGCCAT CAGAGGCTGG GACACCAGAA GGGAATTCAG AGATGGAAAG GACACCGGCT
    CTGACTGGAG ACGTCCAGGT GCTTGTAGGT GAACTTAGGG AGAAGAACGG ACGTTTCCAA
    CGTGAGTTGG CCACTTTGCG GGAAGAGAAT CAATCTTTGA AGCAAAAGCT CATTGCATTG
    GAGAGCGCCA GCTCCAGCAA GCATTCTGTT AACGGAGTTG TCCCAGATAA CATCAATGGA
    AGCGGTCTTA AAACTTCAGT GTCATCTGCA AGTGACATCA AAGTCTCTCC TTTACCCTCT
    TTAGACTCCT CTGAGTTTGA GAAGATCCCC TCACGCTCAG ACTCTGTCAG TAGCAGCATT
    AAGAGCGTGG TGCCCAGCGG TGTGGTGAAA GATCTCTCTG TGGAGTCTCT GACAGAACGC
    ATCCAGAAGA TGGAAGAGAG TCACCACAGT ACGGCCGAAG AGCTTCAGGC CACTCTACAG
    GAGTTGGCTG ACCAGCAACA GGTCGTCCAG GAGCTGACAG CTGAGAATGA GCGTTTGGCA
    GAGGAGAAAG GCCTCTTGCA GACTTCCCTA CAGCAGCAAA GGGAGCGCGT AGAACTCTTG
    GCGCAACAAA ACGAGACTCT TCTCCAGAGG CTCAGAGAAC AAGCTCAGAG TCAAGAAGCT
    GAGGCTTCAC GTGCCTCTCG GATGGCAGAA CTCGAACAGC GTTTGGCTGA GCAAGTAGAG
    AGTTCGCGAT TTGAAAGAGA GAAGCTGGTA GACATCCAGC AGCAGCTGAC AGGGAGTCTC
    AGAGCCTTGG AGAAGGAGAA TCAGGAGGCG CAAACTGCTG TGAAGAGCTT AAGGGAAGAA
    GAAGGGCTTC TGCAAGGGCA TCTGGAGAGT GAGAAGCTTG CTAGAGATGA GGCAGTGAGG
    AAAACTGAGG AACAGAGGCT AGCCATGGAG GCTCTGAGGG TGGACAATGC GAGTATGAAG
    GCGCAGGTGG AGGTGGAGCG GCAGAAGGTT GCTGAGCTGA AGGCAGTGCA GAGCGCTAGT
    GACAACACTG AGCTGCAAAG TCTGCTCAAA GTGGCGCATG AAGATCGAGA CAAGCTGGAG
    ATGACCTGCA CAGAGCTACG ACAAGAGCTG CAGCAGGTGG AGGCAGACAG ACAGCAGCTT
    CTGGAGAAGG TGGAGAAACA GGAACAAGAT CATAAAGCCA ACACAAATTC TCTCGAGGAA
    AAGAGCATAG ATGCTGAGAA CCAAATCAAG GACCTGAAAG AGACCATCTT CGAGTTGGAG
    GACCAGGTGG AGCAGCAACG GGCTGTTAGA CTGCACACCA ACCAGACCAT CCTGGACCTG
    GAGAATCAAA TCAAGAAGCT GGAAGACAAC AAAACTGAAC TGGAGAAGCA GATTAAGGTC
    GTGAATAAAC AAATGAAGGA TGAAACGGAG GAGTGGCGCC GTTTCCAGGC TGACCTCCAG
    ACAGCAGTAG TGGTTGCCAA TGACATAAAG GTGGAGGCTC AGCAGGAGCT GCGGACGCTG
    CGCAGACGTC TGCAGGAGGA GCAGGAGCAC AGCGCGAGAC TGGCTTCAGA GCTGGAGCAG
    ATCCAAGGCA CCAGGTCATG GAATGAAGAT GCCAGCCTCT CCGACTCAGA CGGCAGTCCA
    CACTGGAGCC GAATGACCAT TTCCCAGAAT GCACCTGTGG GTTTCCTAAC CAATGGCAAT
    GGCAGTGGAA GCAGTGTGTC CTCAGAGCCA GAAGCCACGG TCAAATCTCT CATCAAATCA
    TTTGACACCG CAACGCCGAA TGGATCTAGC AGCTCAGTAC AGATACACAC ATCTCCCAGA
    AGCCCCCTGA GTGGCATACC AGTCCGTACA GCTCCCGCTG CTGCCGTCTC ACCCATTCAG
    AGGCACTCTG GAATCAAGCC ACTGTCGAAG ACCCTCGAGA GAAGAATTGC CTTTGGAGAT
    TTCCCACACA ATGGTTCATG TGATGAACTG AAGCCCTCGA CTCTTATGAG GAAGAGCCCG
    TCTCTAGAGT CTGTGATTAA AACTCCAGCT CACTTCAACG GCCGCACTGC ATCGTTTACT
    TACAACAGAG CCAACAGCAA ACTCAGTGTG GAAAGAACAG ACCCTCTGTC CGCTCTGGCG
    CGGGAGTATG GTGGATCTAA GAGGAACGCT CTACTGAAGT GGTGCCAGAA GAAAACAGAG
    GGCTATCCGA ATATAGACGT GACGAACTTC AGCAGCAGCT GGAGCGACGG CCTAGCGTTC
    TGCGCTCTCT TACACACGTA TCTGCCTGCA CACATCCCCT ACCAGGAGCT CATCAGCCAA
    GACAAGGGGC GAAATCTAAC TCTGGCTTTT CAAGCAGCCG AAAGCATTGG TATCAAGCCC
    TCTCTGGACT TCGAGGAATT GATGCGCACC GATCGACCCG ACTGGCAAAG CGTCATGCAA
    TACGTGTCTC AGATTTACAA ATACTTCGAG ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa11131
    Clone ID Related Accession (Same CDS sequence) XM_009301917.2 , XM_009301918.2 , XM_009301917.3 , XM_685735.8 , XM_685735.7
    Accession Version XM_009301918.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product cytospin-B isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301918.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTAAAAG GGGGCACCGC CAAGGTGGCG CTGCCCAGGA TGGGAGCCCC AGAACGTGCA 
    AAGCTGTCCA CCATCAGTTC ACAAAGCACA TACTCTGGCA ACATCGGGAT GAAGACCTCC
    AGGTCGTCTA GCACCATCGC CTCTGACCTG CGACTCAGCA AGATAAAGAG GGCGAGCAGT
    GATGATGCTC TGGCTAAACC TGTCCTGGGT TCAGTGTCCC GCATAAAGAA GACCATCACC
    ACAGGAGCCA TCGCTGAGCT TACAGACAAC CGGCCACGCA GCGCATCAAG TGTCCAGTCA
    ACCAAAAAGT CAGGGATCCC AGCTCCTCGT GAGATCCCCC AAAGCATCTC CAGAGAACGT
    CTCTCATCGC GCGATCAACA GAAAAGCTCT ACAGCCAAAA AGATGGTCAC CAGTTCCAGC
    GCCTCAAGCA TCTCTGCGCT GGCCAAGCAC TCACGTGGCT CTGTCAAGAC CAAAGCGGAG
    TCTGCGGCCT TGGACAAGAC GTTGCTGGAG AGTCAGGTGA GGGAGCTTCT GGCTGAGGCC
    AAAACCAAAG AGTTCGAGAT CAGTAAGCTA CGAACGGAGC TTCAACGGCA TAAAGGGAAG
    GATTCGCCAT CAGAGGCTGG GACACCAGAA GGGAATTCAG AGATGGAAAG GACACCGGCT
    CTGACTGGAG ACGTCCAGGT GCTTGTAGGT GAACTTAGGG AGAAGAACGG ACGTTTCCAA
    CGTGAGTTGG CCACTTTGCG GGAAGAGAAT CAATCTTTGA AGCAAAAGCT CATTGCATTG
    GAGAGCGCCA GCTCCAGCAA GCATTCTGTT AACGGAGTTG TCCCAGATAA CATCAATGGA
    AGCGGTCTTA AAACTTCAGT GTCATCTGCA AGTGACATCA AAGTCTCTCC TTTACCCTCT
    TTAGACTCCT CTGAGTTTGA GAAGATCCCC TCACGCTCAG ACTCTGTCAG TAGCAGCATT
    AAGAGCGTGG TGCCCAGCGG TGTGGTGAAA GATCTCTCTG TGGAGTCTCT GACAGAACGC
    ATCCAGAAGA TGGAAGAGAG TCACCACAGT ACGGCCGAAG AGCTTCAGGC CACTCTACAG
    GAGTTGGCTG ACCAGCAACA GGTCGTCCAG GAGCTGACAG CTGAGAATGA GCGTTTGGCA
    GAGGAGAAAG GCCTCTTGCA GACTTCCCTA CAGCAGCAAA GGGAGCGCGT AGAACTCTTG
    GCGCAACAAA ACGAGACTCT TCTCCAGAGG CTCAGAGAAC AAGCTCAGAG TCAAGAAGCT
    GAGGCTTCAC GTGCCTCTCG GATGGCAGAA CTCGAACAGC GTTTGGCTGA GCAAGTAGAG
    AGTTCGCGAT TTGAAAGAGA GAAGCTGGTA GACATCCAGC AGCAGCTGAC AGGGAGTCTC
    AGAGCCTTGG AGAAGGAGAA TCAGGAGGCG CAAACTGCTG TGAAGAGCTT AAGGGAAGAA
    GAAGGGCTTC TGCAAGGGCA TCTGGAGAGT GAGAAGCTTG CTAGAGATGA GGCAGTGAGG
    AAAACTGAGG AACAGAGGCT AGCCATGGAG GCTCTGAGGG TGGACAATGC GAGTATGAAG
    GCGCAGGTGG AGGTGGAGCG GCAGAAGGTT GCTGAGCTGA AGGCAGTGCA GAGCGCTAGT
    GACAACACTG AGCTGCAAAG TCTGCTCAAA GTGGCGCATG AAGATCGAGA CAAGCTGGAG
    ATGACCTGCA CAGAGCTACG ACAAGAGCTG CAGCAGGTGG AGGCAGACAG ACAGCAGCTT
    CTGGAGAAGG TGGAGAAACA GGAACAAGAT CATAAAGCCA ACACAAATTC TCTCGAGGAA
    AAGAGCATAG ATGCTGAGAA CCAAATCAAG GACCTGAAAG AGACCATCTT CGAGTTGGAG
    GACCAGGTGG AGCAGCAACG GGCTGTTAGA CTGCACACCA ACCAGACCAT CCTGGACCTG
    GAGAATCAAA TCAAGAAGCT GGAAGACAAC AAAACTGAAC TGGAGAAGCA GATTAAGGTC
    GTGAATAAAC AAATGAAGGA TGAAACGGAG GAGTGGCGCC GTTTCCAGGC TGACCTCCAG
    ACAGCAGTAG TGGTTGCCAA TGACATAAAG GTGGAGGCTC AGCAGGAGCT GCGGACGCTG
    CGCAGACGTC TGCAGGAGGA GCAGGAGCAC AGCGCGAGAC TGGCTTCAGA GCTGGAGCAG
    ATCCAAGGCA CCAGGTCATG GAATGAAGAT GCCAGCCTCT CCGACTCAGA CGGCAGTCCA
    CACTGGAGCC GAATGACCAT TTCCCAGAAT GCACCTGTGG GTTTCCTAAC CAATGGCAAT
    GGCAGTGGAA GCAGTGTGTC CTCAGAGCCA GAAGCCACGG TCAAATCTCT CATCAAATCA
    TTTGACACCG CAACGCCGAA TGGATCTAGC AGCTCAGTAC AGATACACAC ATCTCCCAGA
    AGCCCCCTGA GTGGCATACC AGTCCGTACA GCTCCCGCTG CTGCCGTCTC ACCCATTCAG
    AGGCACTCTG GAATCAAGCC ACTGTCGAAG ACCCTCGAGA GAAGAATTGC CTTTGGAGAT
    TTCCCACACA ATGGTTCATG TGATGAACTG AAGCCCTCGA CTCTTATGAG GAAGAGCCCG
    TCTCTAGAGT CTGTGATTAA AACTCCAGCT CACTTCAACG GCCGCACTGC ATCGTTTACT
    TACAACAGAG CCAACAGCAA ACTCAGTGTG GAAAGAACAG ACCCTCTGTC CGCTCTGGCG
    CGGGAGTATG GTGGATCTAA GAGGAACGCT CTACTGAAGT GGTGCCAGAA GAAAACAGAG
    GGCTATCCGA ATATAGACGT GACGAACTTC AGCAGCAGCT GGAGCGACGG CCTAGCGTTC
    TGCGCTCTCT TACACACGTA TCTGCCTGCA CACATCCCCT ACCAGGAGCT CATCAGCCAA
    GACAAGGGGC GAAATCTAAC TCTGGCTTTT CAAGCAGCCG AAAGCATTGG TATCAAGCCC
    TCTCTGGACT TCGAGGAATT GATGCGCACC GATCGACCCG ACTGGCAAAG CGTCATGCAA
    TACGTGTCTC AGATTTACAA ATACTTCGAG ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300193.1
    CDS197..3232
    Translation

    Target ORF information:

    RefSeq Version XM_009301918.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301918.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTAAAAG GGGGCACCGC CAAGGTGGCG CTGCCCAGGA TGGGAGCCCC AGAACGTGCA 
    AAGCTGTCCA CCATCAGTTC ACAAAGCACA TACTCTGGCA ACATCGGGAT GAAGACCTCC
    AGGTCGTCTA GCACCATCGC CTCTGACCTG CGACTCAGCA AGATAAAGAG GGCGAGCAGT
    GATGATGCTC TGGCTAAACC TGTCCTGGGT TCAGTGTCCC GCATAAAGAA GACCATCACC
    ACAGGAGCCA TCGCTGAGCT TACAGACAAC CGGCCACGCA GCGCATCAAG TGTCCAGTCA
    ACCAAAAAGT CAGGGATCCC AGCTCCTCGT GAGATCCCCC AAAGCATCTC CAGAGAACGT
    CTCTCATCGC GCGATCAACA GAAAAGCTCT ACAGCCAAAA AGATGGTCAC CAGTTCCAGC
    GCCTCAAGCA TCTCTGCGCT GGCCAAGCAC TCACGTGGCT CTGTCAAGAC CAAAGCGGAG
    TCTGCGGCCT TGGACAAGAC GTTGCTGGAG AGTCAGGTGA GGGAGCTTCT GGCTGAGGCC
    AAAACCAAAG AGTTCGAGAT CAGTAAGCTA CGAACGGAGC TTCAACGGCA TAAAGGGAAG
    GATTCGCCAT CAGAGGCTGG GACACCAGAA GGGAATTCAG AGATGGAAAG GACACCGGCT
    CTGACTGGAG ACGTCCAGGT GCTTGTAGGT GAACTTAGGG AGAAGAACGG ACGTTTCCAA
    CGTGAGTTGG CCACTTTGCG GGAAGAGAAT CAATCTTTGA AGCAAAAGCT CATTGCATTG
    GAGAGCGCCA GCTCCAGCAA GCATTCTGTT AACGGAGTTG TCCCAGATAA CATCAATGGA
    AGCGGTCTTA AAACTTCAGT GTCATCTGCA AGTGACATCA AAGTCTCTCC TTTACCCTCT
    TTAGACTCCT CTGAGTTTGA GAAGATCCCC TCACGCTCAG ACTCTGTCAG TAGCAGCATT
    AAGAGCGTGG TGCCCAGCGG TGTGGTGAAA GATCTCTCTG TGGAGTCTCT GACAGAACGC
    ATCCAGAAGA TGGAAGAGAG TCACCACAGT ACGGCCGAAG AGCTTCAGGC CACTCTACAG
    GAGTTGGCTG ACCAGCAACA GGTCGTCCAG GAGCTGACAG CTGAGAATGA GCGTTTGGCA
    GAGGAGAAAG GCCTCTTGCA GACTTCCCTA CAGCAGCAAA GGGAGCGCGT AGAACTCTTG
    GCGCAACAAA ACGAGACTCT TCTCCAGAGG CTCAGAGAAC AAGCTCAGAG TCAAGAAGCT
    GAGGCTTCAC GTGCCTCTCG GATGGCAGAA CTCGAACAGC GTTTGGCTGA GCAAGTAGAG
    AGTTCGCGAT TTGAAAGAGA GAAGCTGGTA GACATCCAGC AGCAGCTGAC AGGGAGTCTC
    AGAGCCTTGG AGAAGGAGAA TCAGGAGGCG CAAACTGCTG TGAAGAGCTT AAGGGAAGAA
    GAAGGGCTTC TGCAAGGGCA TCTGGAGAGT GAGAAGCTTG CTAGAGATGA GGCAGTGAGG
    AAAACTGAGG AACAGAGGCT AGCCATGGAG GCTCTGAGGG TGGACAATGC GAGTATGAAG
    GCGCAGGTGG AGGTGGAGCG GCAGAAGGTT GCTGAGCTGA AGGCAGTGCA GAGCGCTAGT
    GACAACACTG AGCTGCAAAG TCTGCTCAAA GTGGCGCATG AAGATCGAGA CAAGCTGGAG
    ATGACCTGCA CAGAGCTACG ACAAGAGCTG CAGCAGGTGG AGGCAGACAG ACAGCAGCTT
    CTGGAGAAGG TGGAGAAACA GGAACAAGAT CATAAAGCCA ACACAAATTC TCTCGAGGAA
    AAGAGCATAG ATGCTGAGAA CCAAATCAAG GACCTGAAAG AGACCATCTT CGAGTTGGAG
    GACCAGGTGG AGCAGCAACG GGCTGTTAGA CTGCACACCA ACCAGACCAT CCTGGACCTG
    GAGAATCAAA TCAAGAAGCT GGAAGACAAC AAAACTGAAC TGGAGAAGCA GATTAAGGTC
    GTGAATAAAC AAATGAAGGA TGAAACGGAG GAGTGGCGCC GTTTCCAGGC TGACCTCCAG
    ACAGCAGTAG TGGTTGCCAA TGACATAAAG GTGGAGGCTC AGCAGGAGCT GCGGACGCTG
    CGCAGACGTC TGCAGGAGGA GCAGGAGCAC AGCGCGAGAC TGGCTTCAGA GCTGGAGCAG
    ATCCAAGGCA CCAGGTCATG GAATGAAGAT GCCAGCCTCT CCGACTCAGA CGGCAGTCCA
    CACTGGAGCC GAATGACCAT TTCCCAGAAT GCACCTGTGG GTTTCCTAAC CAATGGCAAT
    GGCAGTGGAA GCAGTGTGTC CTCAGAGCCA GAAGCCACGG TCAAATCTCT CATCAAATCA
    TTTGACACCG CAACGCCGAA TGGATCTAGC AGCTCAGTAC AGATACACAC ATCTCCCAGA
    AGCCCCCTGA GTGGCATACC AGTCCGTACA GCTCCCGCTG CTGCCGTCTC ACCCATTCAG
    AGGCACTCTG GAATCAAGCC ACTGTCGAAG ACCCTCGAGA GAAGAATTGC CTTTGGAGAT
    TTCCCACACA ATGGTTCATG TGATGAACTG AAGCCCTCGA CTCTTATGAG GAAGAGCCCG
    TCTCTAGAGT CTGTGATTAA AACTCCAGCT CACTTCAACG GCCGCACTGC ATCGTTTACT
    TACAACAGAG CCAACAGCAA ACTCAGTGTG GAAAGAACAG ACCCTCTGTC CGCTCTGGCG
    CGGGAGTATG GTGGATCTAA GAGGAACGCT CTACTGAAGT GGTGCCAGAA GAAAACAGAG
    GGCTATCCGA ATATAGACGT GACGAACTTC AGCAGCAGCT GGAGCGACGG CCTAGCGTTC
    TGCGCTCTCT TACACACGTA TCTGCCTGCA CACATCCCCT ACCAGGAGCT CATCAGCCAA
    GACAAGGGGC GAAATCTAAC TCTGGCTTTT CAAGCAGCCG AAAGCATTGG TATCAAGCCC
    TCTCTGGACT TCGAGGAATT GATGCGCACC GATCGACCCG ACTGGCAAAG CGTCATGCAA
    TACGTGTCTC AGATTTACAA ATACTTCGAG ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa11131
    Clone ID Related Accession (Same CDS sequence) XM_009301917.2 , XM_009301918.2 , XM_009301917.3 , XM_685735.8 , XM_685735.7
    Accession Version XM_009301917.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product cytospin-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301917.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTAAAAG GGGGCACCGC CAAGGTGGCG CTGCCCAGGA TGGGAGCCCC AGAACGTGCA 
    AAGCTGTCCA CCATCAGTTC ACAAAGCACA TACTCTGGCA ACATCGGGAT GAAGACCTCC
    AGGTCGTCTA GCACCATCGC CTCTGACCTG CGACTCAGCA AGATAAAGAG GGCGAGCAGT
    GATGATGCTC TGGCTAAACC TGTCCTGGGT TCAGTGTCCC GCATAAAGAA GACCATCACC
    ACAGGAGCCA TCGCTGAGCT TACAGACAAC CGGCCACGCA GCGCATCAAG TGTCCAGTCA
    ACCAAAAAGT CAGGGATCCC AGCTCCTCGT GAGATCCCCC AAAGCATCTC CAGAGAACGT
    CTCTCATCGC GCGATCAACA GAAAAGCTCT ACAGCCAAAA AGATGGTCAC CAGTTCCAGC
    GCCTCAAGCA TCTCTGCGCT GGCCAAGCAC TCACGTGGCT CTGTCAAGAC CAAAGCGGAG
    TCTGCGGCCT TGGACAAGAC GTTGCTGGAG AGTCAGGTGA GGGAGCTTCT GGCTGAGGCC
    AAAACCAAAG AGTTCGAGAT CAGTAAGCTA CGAACGGAGC TTCAACGGCA TAAAGGGAAG
    GATTCGCCAT CAGAGGCTGG GACACCAGAA GGGAATTCAG AGATGGAAAG GACACCGGCT
    CTGACTGGAG ACGTCCAGGT GCTTGTAGGT GAACTTAGGG AGAAGAACGG ACGTTTCCAA
    CGTGAGTTGG CCACTTTGCG GGAAGAGAAT CAATCTTTGA AGCAAAAGCT CATTGCATTG
    GAGAGCGCCA GCTCCAGCAA GCATTCTGTT AACGGAGTTG TCCCAGATAA CATCAATGGA
    AGCGGTCTTA AAACTTCAGT GTCATCTGCA AGTGACATCA AAGTCTCTCC TTTACCCTCT
    TTAGACTCCT CTGAGTTTGA GAAGATCCCC TCACGCTCAG ACTCTGTCAG TAGCAGCATT
    AAGAGCGTGG TGCCCAGCGG TGTGGTGAAA GATCTCTCTG TGGAGTCTCT GACAGAACGC
    ATCCAGAAGA TGGAAGAGAG TCACCACAGT ACGGCCGAAG AGCTTCAGGC CACTCTACAG
    GAGTTGGCTG ACCAGCAACA GGTCGTCCAG GAGCTGACAG CTGAGAATGA GCGTTTGGCA
    GAGGAGAAAG GCCTCTTGCA GACTTCCCTA CAGCAGCAAA GGGAGCGCGT AGAACTCTTG
    GCGCAACAAA ACGAGACTCT TCTCCAGAGG CTCAGAGAAC AAGCTCAGAG TCAAGAAGCT
    GAGGCTTCAC GTGCCTCTCG GATGGCAGAA CTCGAACAGC GTTTGGCTGA GCAAGTAGAG
    AGTTCGCGAT TTGAAAGAGA GAAGCTGGTA GACATCCAGC AGCAGCTGAC AGGGAGTCTC
    AGAGCCTTGG AGAAGGAGAA TCAGGAGGCG CAAACTGCTG TGAAGAGCTT AAGGGAAGAA
    GAAGGGCTTC TGCAAGGGCA TCTGGAGAGT GAGAAGCTTG CTAGAGATGA GGCAGTGAGG
    AAAACTGAGG AACAGAGGCT AGCCATGGAG GCTCTGAGGG TGGACAATGC GAGTATGAAG
    GCGCAGGTGG AGGTGGAGCG GCAGAAGGTT GCTGAGCTGA AGGCAGTGCA GAGCGCTAGT
    GACAACACTG AGCTGCAAAG TCTGCTCAAA GTGGCGCATG AAGATCGAGA CAAGCTGGAG
    ATGACCTGCA CAGAGCTACG ACAAGAGCTG CAGCAGGTGG AGGCAGACAG ACAGCAGCTT
    CTGGAGAAGG TGGAGAAACA GGAACAAGAT CATAAAGCCA ACACAAATTC TCTCGAGGAA
    AAGAGCATAG ATGCTGAGAA CCAAATCAAG GACCTGAAAG AGACCATCTT CGAGTTGGAG
    GACCAGGTGG AGCAGCAACG GGCTGTTAGA CTGCACACCA ACCAGACCAT CCTGGACCTG
    GAGAATCAAA TCAAGAAGCT GGAAGACAAC AAAACTGAAC TGGAGAAGCA GATTAAGGTC
    GTGAATAAAC AAATGAAGGA TGAAACGGAG GAGTGGCGCC GTTTCCAGGC TGACCTCCAG
    ACAGCAGTAG TGGTTGCCAA TGACATAAAG GTGGAGGCTC AGCAGGAGCT GCGGACGCTG
    CGCAGACGTC TGCAGGAGGA GCAGGAGCAC AGCGCGAGAC TGGCTTCAGA GCTGGAGCAG
    ATCCAAGGCA CCAGGTCATG GAATGAAGAT GCCAGCCTCT CCGACTCAGA CGGCAGTCCA
    CACTGGAGCC GAATGACCAT TTCCCAGAAT GCACCTGTGG GTTTCCTAAC CAATGGCAAT
    GGCAGTGGAA GCAGTGTGTC CTCAGAGCCA GAAGCCACGG TCAAATCTCT CATCAAATCA
    TTTGACACCG CAACGCCGAA TGGATCTAGC AGCTCAGTAC AGATACACAC ATCTCCCAGA
    AGCCCCCTGA GTGGCATACC AGTCCGTACA GCTCCCGCTG CTGCCGTCTC ACCCATTCAG
    AGGCACTCTG GAATCAAGCC ACTGTCGAAG ACCCTCGAGA GAAGAATTGC CTTTGGAGAT
    TTCCCACACA ATGGTTCATG TGATGAACTG AAGCCCTCGA CTCTTATGAG GAAGAGCCCG
    TCTCTAGAGT CTGTGATTAA AACTCCAGCT CACTTCAACG GCCGCACTGC ATCGTTTACT
    TACAACAGAG CCAACAGCAA ACTCAGTGTG GAAAGAACAG ACCCTCTGTC CGCTCTGGCG
    CGGGAGTATG GTGGATCTAA GAGGAACGCT CTACTGAAGT GGTGCCAGAA GAAAACAGAG
    GGCTATCCGA ATATAGACGT GACGAACTTC AGCAGCAGCT GGAGCGACGG CCTAGCGTTC
    TGCGCTCTCT TACACACGTA TCTGCCTGCA CACATCCCCT ACCAGGAGCT CATCAGCCAA
    GACAAGGGGC GAAATCTAAC TCTGGCTTTT CAAGCAGCCG AAAGCATTGG TATCAAGCCC
    TCTCTGGACT TCGAGGAATT GATGCGCACC GATCGACCCG ACTGGCAAAG CGTCATGCAA
    TACGTGTCTC AGATTTACAA ATACTTCGAG ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300192.1
    CDS292..3327
    Translation

    Target ORF information:

    RefSeq Version XM_009301917.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301917.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTAAAAG GGGGCACCGC CAAGGTGGCG CTGCCCAGGA TGGGAGCCCC AGAACGTGCA 
    AAGCTGTCCA CCATCAGTTC ACAAAGCACA TACTCTGGCA ACATCGGGAT GAAGACCTCC
    AGGTCGTCTA GCACCATCGC CTCTGACCTG CGACTCAGCA AGATAAAGAG GGCGAGCAGT
    GATGATGCTC TGGCTAAACC TGTCCTGGGT TCAGTGTCCC GCATAAAGAA GACCATCACC
    ACAGGAGCCA TCGCTGAGCT TACAGACAAC CGGCCACGCA GCGCATCAAG TGTCCAGTCA
    ACCAAAAAGT CAGGGATCCC AGCTCCTCGT GAGATCCCCC AAAGCATCTC CAGAGAACGT
    CTCTCATCGC GCGATCAACA GAAAAGCTCT ACAGCCAAAA AGATGGTCAC CAGTTCCAGC
    GCCTCAAGCA TCTCTGCGCT GGCCAAGCAC TCACGTGGCT CTGTCAAGAC CAAAGCGGAG
    TCTGCGGCCT TGGACAAGAC GTTGCTGGAG AGTCAGGTGA GGGAGCTTCT GGCTGAGGCC
    AAAACCAAAG AGTTCGAGAT CAGTAAGCTA CGAACGGAGC TTCAACGGCA TAAAGGGAAG
    GATTCGCCAT CAGAGGCTGG GACACCAGAA GGGAATTCAG AGATGGAAAG GACACCGGCT
    CTGACTGGAG ACGTCCAGGT GCTTGTAGGT GAACTTAGGG AGAAGAACGG ACGTTTCCAA
    CGTGAGTTGG CCACTTTGCG GGAAGAGAAT CAATCTTTGA AGCAAAAGCT CATTGCATTG
    GAGAGCGCCA GCTCCAGCAA GCATTCTGTT AACGGAGTTG TCCCAGATAA CATCAATGGA
    AGCGGTCTTA AAACTTCAGT GTCATCTGCA AGTGACATCA AAGTCTCTCC TTTACCCTCT
    TTAGACTCCT CTGAGTTTGA GAAGATCCCC TCACGCTCAG ACTCTGTCAG TAGCAGCATT
    AAGAGCGTGG TGCCCAGCGG TGTGGTGAAA GATCTCTCTG TGGAGTCTCT GACAGAACGC
    ATCCAGAAGA TGGAAGAGAG TCACCACAGT ACGGCCGAAG AGCTTCAGGC CACTCTACAG
    GAGTTGGCTG ACCAGCAACA GGTCGTCCAG GAGCTGACAG CTGAGAATGA GCGTTTGGCA
    GAGGAGAAAG GCCTCTTGCA GACTTCCCTA CAGCAGCAAA GGGAGCGCGT AGAACTCTTG
    GCGCAACAAA ACGAGACTCT TCTCCAGAGG CTCAGAGAAC AAGCTCAGAG TCAAGAAGCT
    GAGGCTTCAC GTGCCTCTCG GATGGCAGAA CTCGAACAGC GTTTGGCTGA GCAAGTAGAG
    AGTTCGCGAT TTGAAAGAGA GAAGCTGGTA GACATCCAGC AGCAGCTGAC AGGGAGTCTC
    AGAGCCTTGG AGAAGGAGAA TCAGGAGGCG CAAACTGCTG TGAAGAGCTT AAGGGAAGAA
    GAAGGGCTTC TGCAAGGGCA TCTGGAGAGT GAGAAGCTTG CTAGAGATGA GGCAGTGAGG
    AAAACTGAGG AACAGAGGCT AGCCATGGAG GCTCTGAGGG TGGACAATGC GAGTATGAAG
    GCGCAGGTGG AGGTGGAGCG GCAGAAGGTT GCTGAGCTGA AGGCAGTGCA GAGCGCTAGT
    GACAACACTG AGCTGCAAAG TCTGCTCAAA GTGGCGCATG AAGATCGAGA CAAGCTGGAG
    ATGACCTGCA CAGAGCTACG ACAAGAGCTG CAGCAGGTGG AGGCAGACAG ACAGCAGCTT
    CTGGAGAAGG TGGAGAAACA GGAACAAGAT CATAAAGCCA ACACAAATTC TCTCGAGGAA
    AAGAGCATAG ATGCTGAGAA CCAAATCAAG GACCTGAAAG AGACCATCTT CGAGTTGGAG
    GACCAGGTGG AGCAGCAACG GGCTGTTAGA CTGCACACCA ACCAGACCAT CCTGGACCTG
    GAGAATCAAA TCAAGAAGCT GGAAGACAAC AAAACTGAAC TGGAGAAGCA GATTAAGGTC
    GTGAATAAAC AAATGAAGGA TGAAACGGAG GAGTGGCGCC GTTTCCAGGC TGACCTCCAG
    ACAGCAGTAG TGGTTGCCAA TGACATAAAG GTGGAGGCTC AGCAGGAGCT GCGGACGCTG
    CGCAGACGTC TGCAGGAGGA GCAGGAGCAC AGCGCGAGAC TGGCTTCAGA GCTGGAGCAG
    ATCCAAGGCA CCAGGTCATG GAATGAAGAT GCCAGCCTCT CCGACTCAGA CGGCAGTCCA
    CACTGGAGCC GAATGACCAT TTCCCAGAAT GCACCTGTGG GTTTCCTAAC CAATGGCAAT
    GGCAGTGGAA GCAGTGTGTC CTCAGAGCCA GAAGCCACGG TCAAATCTCT CATCAAATCA
    TTTGACACCG CAACGCCGAA TGGATCTAGC AGCTCAGTAC AGATACACAC ATCTCCCAGA
    AGCCCCCTGA GTGGCATACC AGTCCGTACA GCTCCCGCTG CTGCCGTCTC ACCCATTCAG
    AGGCACTCTG GAATCAAGCC ACTGTCGAAG ACCCTCGAGA GAAGAATTGC CTTTGGAGAT
    TTCCCACACA ATGGTTCATG TGATGAACTG AAGCCCTCGA CTCTTATGAG GAAGAGCCCG
    TCTCTAGAGT CTGTGATTAA AACTCCAGCT CACTTCAACG GCCGCACTGC ATCGTTTACT
    TACAACAGAG CCAACAGCAA ACTCAGTGTG GAAAGAACAG ACCCTCTGTC CGCTCTGGCG
    CGGGAGTATG GTGGATCTAA GAGGAACGCT CTACTGAAGT GGTGCCAGAA GAAAACAGAG
    GGCTATCCGA ATATAGACGT GACGAACTTC AGCAGCAGCT GGAGCGACGG CCTAGCGTTC
    TGCGCTCTCT TACACACGTA TCTGCCTGCA CACATCCCCT ACCAGGAGCT CATCAGCCAA
    GACAAGGGGC GAAATCTAAC TCTGGCTTTT CAAGCAGCCG AAAGCATTGG TATCAAGCCC
    TCTCTGGACT TCGAGGAATT GATGCGCACC GATCGACCCG ACTGGCAAAG CGTCATGCAA
    TACGTGTCTC AGATTTACAA ATACTTCGAG ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa11131
    Clone ID Related Accession (Same CDS sequence) XM_009301917.2 , XM_009301918.2 , XM_009301917.3 , XM_685735.8 , XM_685735.7
    Accession Version XM_685735.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product cytospin-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_685735.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTAAAAG GGGGCACCGC CAAGGTGGCG CTGCCCAGGA TGGGAGCCCC AGAACGTGCA 
    AAGCTGTCCA CCATCAGTTC ACAAAGCACA TACTCTGGCA ACATCGGGAT GAAGACCTCC
    AGGTCGTCTA GCACCATCGC CTCTGACCTG CGACTCAGCA AGATAAAGAG GGCGAGCAGT
    GATGATGCTC TGGCTAAACC TGTCCTGGGT TCAGTGTCCC GCATAAAGAA GACCATCACC
    ACAGGAGCCA TCGCTGAGCT TACAGACAAC CGGCCACGCA GCGCATCAAG TGTCCAGTCA
    ACCAAAAAGT CAGGGATCCC AGCTCCTCGT GAGATCCCCC AAAGCATCTC CAGAGAACGT
    CTCTCATCGC GCGATCAACA GAAAAGCTCT ACAGCCAAAA AGATGGTCAC CAGTTCCAGC
    GCCTCAAGCA TCTCTGCGCT GGCCAAGCAC TCACGTGGCT CTGTCAAGAC CAAAGCGGAG
    TCTGCGGCCT TGGACAAGAC GTTGCTGGAG AGTCAGGTGA GGGAGCTTCT GGCTGAGGCC
    AAAACCAAAG AGTTCGAGAT CAGTAAGCTA CGAACGGAGC TTCAACGGCA TAAAGGGAAG
    GATTCGCCAT CAGAGGCTGG GACACCAGAA GGGAATTCAG AGATGGAAAG GACACCGGCT
    CTGACTGGAG ACGTCCAGGT GCTTGTAGGT GAACTTAGGG AGAAGAACGG ACGTTTCCAA
    CGTGAGTTGG CCACTTTGCG GGAAGAGAAT CAATCTTTGA AGCAAAAGCT CATTGCATTG
    GAGAGCGCCA GCTCCAGCAA GCATTCTGTT AACGGAGTTG TCCCAGATAA CATCAATGGA
    AGCGGTCTTA AAACTTCAGT GTCATCTGCA AGTGACATCA AAGTCTCTCC TTTACCCTCT
    TTAGACTCCT CTGAGTTTGA GAAGATCCCC TCACGCTCAG ACTCTGTCAG TAGCAGCATT
    AAGAGCGTGG TGCCCAGCGG TGTGGTGAAA GATCTCTCTG TGGAGTCTCT GACAGAACGC
    ATCCAGAAGA TGGAAGAGAG TCACCACAGT ACGGCCGAAG AGCTTCAGGC CACTCTACAG
    GAGTTGGCTG ACCAGCAACA GGTCGTCCAG GAGCTGACAG CTGAGAATGA GCGTTTGGCA
    GAGGAGAAAG GCCTCTTGCA GACTTCCCTA CAGCAGCAAA GGGAGCGCGT AGAACTCTTG
    GCGCAACAAA ACGAGACTCT TCTCCAGAGG CTCAGAGAAC AAGCTCAGAG TCAAGAAGCT
    GAGGCTTCAC GTGCCTCTCG GATGGCAGAA CTCGAACAGC GTTTGGCTGA GCAAGTAGAG
    AGTTCGCGAT TTGAAAGAGA GAAGCTGGTA GACATCCAGC AGCAGCTGAC AGGGAGTCTC
    AGAGCCTTGG AGAAGGAGAA TCAGGAGGCG CAAACTGCTG TGAAGAGCTT AAGGGAAGAA
    GAAGGGCTTC TGCAAGGGCA TCTGGAGAGT GAGAAGCTTG CTAGAGATGA GGCAGTGAGG
    AAAACTGAGG AACAGAGGCT AGCCATGGAG GCTCTGAGGG TGGACAATGC GAGTATGAAG
    GCGCAGGTGG AGGTGGAGCG GCAGAAGGTT GCTGAGCTGA AGGCAGTGCA GAGCGCTAGT
    GACAACACTG AGCTGCAAAG TCTGCTCAAA GTGGCGCATG AAGATCGAGA CAAGCTGGAG
    ATGACCTGCA CAGAGCTACG ACAAGAGCTG CAGCAGGTGG AGGCAGACAG ACAGCAGCTT
    CTGGAGAAGG TGGAGAAACA GGAACAAGAT CATAAAGCCA ACACAAATTC TCTCGAGGAA
    AAGAGCATAG ATGCTGAGAA CCAAATCAAG GACCTGAAAG AGACCATCTT CGAGTTGGAG
    GACCAGGTGG AGCAGCAACG GGCTGTTAGA CTGCACACCA ACCAGACCAT CCTGGACCTG
    GAGAATCAAA TCAAGAAGCT GGAAGACAAC AAAACTGAAC TGGAGAAGCA GATTAAGGTC
    GTGAATAAAC AAATGAAGGA TGAAACGGAG GAGTGGCGCC GTTTCCAGGC TGACCTCCAG
    ACAGCAGTAG TGGTTGCCAA TGACATAAAG GTGGAGGCTC AGCAGGAGCT GCGGACGCTG
    CGCAGACGTC TGCAGGAGGA GCAGGAGCAC AGCGCGAGAC TGGCTTCAGA GCTGGAGCAG
    ATCCAAGGCA CCAGGTCATG GAATGAAGAT GCCAGCCTCT CCGACTCAGA CGGCAGTCCA
    CACTGGAGCC GAATGACCAT TTCCCAGAAT GCACCTGTGG GTTTCCTAAC CAATGGCAAT
    GGCAGTGGAA GCAGTGTGTC CTCAGAGCCA GAAGCCACGG TCAAATCTCT CATCAAATCA
    TTTGACACCG CAACGCCGAA TGGATCTAGC AGCTCAGTAC AGATACACAC ATCTCCCAGA
    AGCCCCCTGA GTGGCATACC AGTCCGTACA GCTCCCGCTG CTGCCGTCTC ACCCATTCAG
    AGGCACTCTG GAATCAAGCC ACTGTCGAAG ACCCTCGAGA GAAGAATTGC CTTTGGAGAT
    TTCCCACACA ATGGTTCATG TGATGAACTG AAGCCCTCGA CTCTTATGAG GAAGAGCCCG
    TCTCTAGAGT CTGTGATTAA AACTCCAGCT CACTTCAACG GCCGCACTGC ATCGTTTACT
    TACAACAGAG CCAACAGCAA ACTCAGTGTG GAAAGAACAG ACCCTCTGTC CGCTCTGGCG
    CGGGAGTATG GTGGATCTAA GAGGAACGCT CTACTGAAGT GGTGCCAGAA GAAAACAGAG
    GGCTATCCGA ATATAGACGT GACGAACTTC AGCAGCAGCT GGAGCGACGG CCTAGCGTTC
    TGCGCTCTCT TACACACGTA TCTGCCTGCA CACATCCCCT ACCAGGAGCT CATCAGCCAA
    GACAAGGGGC GAAATCTAAC TCTGGCTTTT CAAGCAGCCG AAAGCATTGG TATCAAGCCC
    TCTCTGGACT TCGAGGAATT GATGCGCACC GATCGACCCG ACTGGCAAAG CGTCATGCAA
    TACGTGTCTC AGATTTACAA ATACTTCGAG ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_690827.4
    CDS107..3142
    Translation

    Target ORF information:

    RefSeq Version XM_685735.8
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_685735.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTAAAAG GGGGCACCGC CAAGGTGGCG CTGCCCAGGA TGGGAGCCCC AGAACGTGCA 
    AAGCTGTCCA CCATCAGTTC ACAAAGCACA TACTCTGGCA ACATCGGGAT GAAGACCTCC
    AGGTCGTCTA GCACCATCGC CTCTGACCTG CGACTCAGCA AGATAAAGAG GGCGAGCAGT
    GATGATGCTC TGGCTAAACC TGTCCTGGGT TCAGTGTCCC GCATAAAGAA GACCATCACC
    ACAGGAGCCA TCGCTGAGCT TACAGACAAC CGGCCACGCA GCGCATCAAG TGTCCAGTCA
    ACCAAAAAGT CAGGGATCCC AGCTCCTCGT GAGATCCCCC AAAGCATCTC CAGAGAACGT
    CTCTCATCGC GCGATCAACA GAAAAGCTCT ACAGCCAAAA AGATGGTCAC CAGTTCCAGC
    GCCTCAAGCA TCTCTGCGCT GGCCAAGCAC TCACGTGGCT CTGTCAAGAC CAAAGCGGAG
    TCTGCGGCCT TGGACAAGAC GTTGCTGGAG AGTCAGGTGA GGGAGCTTCT GGCTGAGGCC
    AAAACCAAAG AGTTCGAGAT CAGTAAGCTA CGAACGGAGC TTCAACGGCA TAAAGGGAAG
    GATTCGCCAT CAGAGGCTGG GACACCAGAA GGGAATTCAG AGATGGAAAG GACACCGGCT
    CTGACTGGAG ACGTCCAGGT GCTTGTAGGT GAACTTAGGG AGAAGAACGG ACGTTTCCAA
    CGTGAGTTGG CCACTTTGCG GGAAGAGAAT CAATCTTTGA AGCAAAAGCT CATTGCATTG
    GAGAGCGCCA GCTCCAGCAA GCATTCTGTT AACGGAGTTG TCCCAGATAA CATCAATGGA
    AGCGGTCTTA AAACTTCAGT GTCATCTGCA AGTGACATCA AAGTCTCTCC TTTACCCTCT
    TTAGACTCCT CTGAGTTTGA GAAGATCCCC TCACGCTCAG ACTCTGTCAG TAGCAGCATT
    AAGAGCGTGG TGCCCAGCGG TGTGGTGAAA GATCTCTCTG TGGAGTCTCT GACAGAACGC
    ATCCAGAAGA TGGAAGAGAG TCACCACAGT ACGGCCGAAG AGCTTCAGGC CACTCTACAG
    GAGTTGGCTG ACCAGCAACA GGTCGTCCAG GAGCTGACAG CTGAGAATGA GCGTTTGGCA
    GAGGAGAAAG GCCTCTTGCA GACTTCCCTA CAGCAGCAAA GGGAGCGCGT AGAACTCTTG
    GCGCAACAAA ACGAGACTCT TCTCCAGAGG CTCAGAGAAC AAGCTCAGAG TCAAGAAGCT
    GAGGCTTCAC GTGCCTCTCG GATGGCAGAA CTCGAACAGC GTTTGGCTGA GCAAGTAGAG
    AGTTCGCGAT TTGAAAGAGA GAAGCTGGTA GACATCCAGC AGCAGCTGAC AGGGAGTCTC
    AGAGCCTTGG AGAAGGAGAA TCAGGAGGCG CAAACTGCTG TGAAGAGCTT AAGGGAAGAA
    GAAGGGCTTC TGCAAGGGCA TCTGGAGAGT GAGAAGCTTG CTAGAGATGA GGCAGTGAGG
    AAAACTGAGG AACAGAGGCT AGCCATGGAG GCTCTGAGGG TGGACAATGC GAGTATGAAG
    GCGCAGGTGG AGGTGGAGCG GCAGAAGGTT GCTGAGCTGA AGGCAGTGCA GAGCGCTAGT
    GACAACACTG AGCTGCAAAG TCTGCTCAAA GTGGCGCATG AAGATCGAGA CAAGCTGGAG
    ATGACCTGCA CAGAGCTACG ACAAGAGCTG CAGCAGGTGG AGGCAGACAG ACAGCAGCTT
    CTGGAGAAGG TGGAGAAACA GGAACAAGAT CATAAAGCCA ACACAAATTC TCTCGAGGAA
    AAGAGCATAG ATGCTGAGAA CCAAATCAAG GACCTGAAAG AGACCATCTT CGAGTTGGAG
    GACCAGGTGG AGCAGCAACG GGCTGTTAGA CTGCACACCA ACCAGACCAT CCTGGACCTG
    GAGAATCAAA TCAAGAAGCT GGAAGACAAC AAAACTGAAC TGGAGAAGCA GATTAAGGTC
    GTGAATAAAC AAATGAAGGA TGAAACGGAG GAGTGGCGCC GTTTCCAGGC TGACCTCCAG
    ACAGCAGTAG TGGTTGCCAA TGACATAAAG GTGGAGGCTC AGCAGGAGCT GCGGACGCTG
    CGCAGACGTC TGCAGGAGGA GCAGGAGCAC AGCGCGAGAC TGGCTTCAGA GCTGGAGCAG
    ATCCAAGGCA CCAGGTCATG GAATGAAGAT GCCAGCCTCT CCGACTCAGA CGGCAGTCCA
    CACTGGAGCC GAATGACCAT TTCCCAGAAT GCACCTGTGG GTTTCCTAAC CAATGGCAAT
    GGCAGTGGAA GCAGTGTGTC CTCAGAGCCA GAAGCCACGG TCAAATCTCT CATCAAATCA
    TTTGACACCG CAACGCCGAA TGGATCTAGC AGCTCAGTAC AGATACACAC ATCTCCCAGA
    AGCCCCCTGA GTGGCATACC AGTCCGTACA GCTCCCGCTG CTGCCGTCTC ACCCATTCAG
    AGGCACTCTG GAATCAAGCC ACTGTCGAAG ACCCTCGAGA GAAGAATTGC CTTTGGAGAT
    TTCCCACACA ATGGTTCATG TGATGAACTG AAGCCCTCGA CTCTTATGAG GAAGAGCCCG
    TCTCTAGAGT CTGTGATTAA AACTCCAGCT CACTTCAACG GCCGCACTGC ATCGTTTACT
    TACAACAGAG CCAACAGCAA ACTCAGTGTG GAAAGAACAG ACCCTCTGTC CGCTCTGGCG
    CGGGAGTATG GTGGATCTAA GAGGAACGCT CTACTGAAGT GGTGCCAGAA GAAAACAGAG
    GGCTATCCGA ATATAGACGT GACGAACTTC AGCAGCAGCT GGAGCGACGG CCTAGCGTTC
    TGCGCTCTCT TACACACGTA TCTGCCTGCA CACATCCCCT ACCAGGAGCT CATCAGCCAA
    GACAAGGGGC GAAATCTAAC TCTGGCTTTT CAAGCAGCCG AAAGCATTGG TATCAAGCCC
    TCTCTGGACT TCGAGGAATT GATGCGCACC GATCGACCCG ACTGGCAAAG CGTCATGCAA
    TACGTGTCTC AGATTTACAA ATACTTCGAG ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa11131
    Clone ID Related Accession (Same CDS sequence) XM_009301917.2 , XM_009301918.2 , XM_009301917.3 , XM_685735.8 , XM_685735.7
    Accession Version XM_685735.7 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product cytospin-B isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_685735.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTAAAAG GGGGCACCGC CAAGGTGGCG CTGCCCAGGA TGGGAGCCCC AGAACGTGCA 
    AAGCTGTCCA CCATCAGTTC ACAAAGCACA TACTCTGGCA ACATCGGGAT GAAGACCTCC
    AGGTCGTCTA GCACCATCGC CTCTGACCTG CGACTCAGCA AGATAAAGAG GGCGAGCAGT
    GATGATGCTC TGGCTAAACC TGTCCTGGGT TCAGTGTCCC GCATAAAGAA GACCATCACC
    ACAGGAGCCA TCGCTGAGCT TACAGACAAC CGGCCACGCA GCGCATCAAG TGTCCAGTCA
    ACCAAAAAGT CAGGGATCCC AGCTCCTCGT GAGATCCCCC AAAGCATCTC CAGAGAACGT
    CTCTCATCGC GCGATCAACA GAAAAGCTCT ACAGCCAAAA AGATGGTCAC CAGTTCCAGC
    GCCTCAAGCA TCTCTGCGCT GGCCAAGCAC TCACGTGGCT CTGTCAAGAC CAAAGCGGAG
    TCTGCGGCCT TGGACAAGAC GTTGCTGGAG AGTCAGGTGA GGGAGCTTCT GGCTGAGGCC
    AAAACCAAAG AGTTCGAGAT CAGTAAGCTA CGAACGGAGC TTCAACGGCA TAAAGGGAAG
    GATTCGCCAT CAGAGGCTGG GACACCAGAA GGGAATTCAG AGATGGAAAG GACACCGGCT
    CTGACTGGAG ACGTCCAGGT GCTTGTAGGT GAACTTAGGG AGAAGAACGG ACGTTTCCAA
    CGTGAGTTGG CCACTTTGCG GGAAGAGAAT CAATCTTTGA AGCAAAAGCT CATTGCATTG
    GAGAGCGCCA GCTCCAGCAA GCATTCTGTT AACGGAGTTG TCCCAGATAA CATCAATGGA
    AGCGGTCTTA AAACTTCAGT GTCATCTGCA AGTGACATCA AAGTCTCTCC TTTACCCTCT
    TTAGACTCCT CTGAGTTTGA GAAGATCCCC TCACGCTCAG ACTCTGTCAG TAGCAGCATT
    AAGAGCGTGG TGCCCAGCGG TGTGGTGAAA GATCTCTCTG TGGAGTCTCT GACAGAACGC
    ATCCAGAAGA TGGAAGAGAG TCACCACAGT ACGGCCGAAG AGCTTCAGGC CACTCTACAG
    GAGTTGGCTG ACCAGCAACA GGTCGTCCAG GAGCTGACAG CTGAGAATGA GCGTTTGGCA
    GAGGAGAAAG GCCTCTTGCA GACTTCCCTA CAGCAGCAAA GGGAGCGCGT AGAACTCTTG
    GCGCAACAAA ACGAGACTCT TCTCCAGAGG CTCAGAGAAC AAGCTCAGAG TCAAGAAGCT
    GAGGCTTCAC GTGCCTCTCG GATGGCAGAA CTCGAACAGC GTTTGGCTGA GCAAGTAGAG
    AGTTCGCGAT TTGAAAGAGA GAAGCTGGTA GACATCCAGC AGCAGCTGAC AGGGAGTCTC
    AGAGCCTTGG AGAAGGAGAA TCAGGAGGCG CAAACTGCTG TGAAGAGCTT AAGGGAAGAA
    GAAGGGCTTC TGCAAGGGCA TCTGGAGAGT GAGAAGCTTG CTAGAGATGA GGCAGTGAGG
    AAAACTGAGG AACAGAGGCT AGCCATGGAG GCTCTGAGGG TGGACAATGC GAGTATGAAG
    GCGCAGGTGG AGGTGGAGCG GCAGAAGGTT GCTGAGCTGA AGGCAGTGCA GAGCGCTAGT
    GACAACACTG AGCTGCAAAG TCTGCTCAAA GTGGCGCATG AAGATCGAGA CAAGCTGGAG
    ATGACCTGCA CAGAGCTACG ACAAGAGCTG CAGCAGGTGG AGGCAGACAG ACAGCAGCTT
    CTGGAGAAGG TGGAGAAACA GGAACAAGAT CATAAAGCCA ACACAAATTC TCTCGAGGAA
    AAGAGCATAG ATGCTGAGAA CCAAATCAAG GACCTGAAAG AGACCATCTT CGAGTTGGAG
    GACCAGGTGG AGCAGCAACG GGCTGTTAGA CTGCACACCA ACCAGACCAT CCTGGACCTG
    GAGAATCAAA TCAAGAAGCT GGAAGACAAC AAAACTGAAC TGGAGAAGCA GATTAAGGTC
    GTGAATAAAC AAATGAAGGA TGAAACGGAG GAGTGGCGCC GTTTCCAGGC TGACCTCCAG
    ACAGCAGTAG TGGTTGCCAA TGACATAAAG GTGGAGGCTC AGCAGGAGCT GCGGACGCTG
    CGCAGACGTC TGCAGGAGGA GCAGGAGCAC AGCGCGAGAC TGGCTTCAGA GCTGGAGCAG
    ATCCAAGGCA CCAGGTCATG GAATGAAGAT GCCAGCCTCT CCGACTCAGA CGGCAGTCCA
    CACTGGAGCC GAATGACCAT TTCCCAGAAT GCACCTGTGG GTTTCCTAAC CAATGGCAAT
    GGCAGTGGAA GCAGTGTGTC CTCAGAGCCA GAAGCCACGG TCAAATCTCT CATCAAATCA
    TTTGACACCG CAACGCCGAA TGGATCTAGC AGCTCAGTAC AGATACACAC ATCTCCCAGA
    AGCCCCCTGA GTGGCATACC AGTCCGTACA GCTCCCGCTG CTGCCGTCTC ACCCATTCAG
    AGGCACTCTG GAATCAAGCC ACTGTCGAAG ACCCTCGAGA GAAGAATTGC CTTTGGAGAT
    TTCCCACACA ATGGTTCATG TGATGAACTG AAGCCCTCGA CTCTTATGAG GAAGAGCCCG
    TCTCTAGAGT CTGTGATTAA AACTCCAGCT CACTTCAACG GCCGCACTGC ATCGTTTACT
    TACAACAGAG CCAACAGCAA ACTCAGTGTG GAAAGAACAG ACCCTCTGTC CGCTCTGGCG
    CGGGAGTATG GTGGATCTAA GAGGAACGCT CTACTGAAGT GGTGCCAGAA GAAAACAGAG
    GGCTATCCGA ATATAGACGT GACGAACTTC AGCAGCAGCT GGAGCGACGG CCTAGCGTTC
    TGCGCTCTCT TACACACGTA TCTGCCTGCA CACATCCCCT ACCAGGAGCT CATCAGCCAA
    GACAAGGGGC GAAATCTAAC TCTGGCTTTT CAAGCAGCCG AAAGCATTGG TATCAAGCCC
    TCTCTGGACT TCGAGGAATT GATGCGCACC GATCGACCCG ACTGGCAAAG CGTCATGCAA
    TACGTGTCTC AGATTTACAA ATACTTCGAG ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_690827.4
    CDS106..3141
    Translation

    Target ORF information:

    RefSeq Version XM_685735.7
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sperm antigen with calponin homology and coiled-coil domains 1 (specc1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_685735.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTAAAAG GGGGCACCGC CAAGGTGGCG CTGCCCAGGA TGGGAGCCCC AGAACGTGCA 
    AAGCTGTCCA CCATCAGTTC ACAAAGCACA TACTCTGGCA ACATCGGGAT GAAGACCTCC
    AGGTCGTCTA GCACCATCGC CTCTGACCTG CGACTCAGCA AGATAAAGAG GGCGAGCAGT
    GATGATGCTC TGGCTAAACC TGTCCTGGGT TCAGTGTCCC GCATAAAGAA GACCATCACC
    ACAGGAGCCA TCGCTGAGCT TACAGACAAC CGGCCACGCA GCGCATCAAG TGTCCAGTCA
    ACCAAAAAGT CAGGGATCCC AGCTCCTCGT GAGATCCCCC AAAGCATCTC CAGAGAACGT
    CTCTCATCGC GCGATCAACA GAAAAGCTCT ACAGCCAAAA AGATGGTCAC CAGTTCCAGC
    GCCTCAAGCA TCTCTGCGCT GGCCAAGCAC TCACGTGGCT CTGTCAAGAC CAAAGCGGAG
    TCTGCGGCCT TGGACAAGAC GTTGCTGGAG AGTCAGGTGA GGGAGCTTCT GGCTGAGGCC
    AAAACCAAAG AGTTCGAGAT CAGTAAGCTA CGAACGGAGC TTCAACGGCA TAAAGGGAAG
    GATTCGCCAT CAGAGGCTGG GACACCAGAA GGGAATTCAG AGATGGAAAG GACACCGGCT
    CTGACTGGAG ACGTCCAGGT GCTTGTAGGT GAACTTAGGG AGAAGAACGG ACGTTTCCAA
    CGTGAGTTGG CCACTTTGCG GGAAGAGAAT CAATCTTTGA AGCAAAAGCT CATTGCATTG
    GAGAGCGCCA GCTCCAGCAA GCATTCTGTT AACGGAGTTG TCCCAGATAA CATCAATGGA
    AGCGGTCTTA AAACTTCAGT GTCATCTGCA AGTGACATCA AAGTCTCTCC TTTACCCTCT
    TTAGACTCCT CTGAGTTTGA GAAGATCCCC TCACGCTCAG ACTCTGTCAG TAGCAGCATT
    AAGAGCGTGG TGCCCAGCGG TGTGGTGAAA GATCTCTCTG TGGAGTCTCT GACAGAACGC
    ATCCAGAAGA TGGAAGAGAG TCACCACAGT ACGGCCGAAG AGCTTCAGGC CACTCTACAG
    GAGTTGGCTG ACCAGCAACA GGTCGTCCAG GAGCTGACAG CTGAGAATGA GCGTTTGGCA
    GAGGAGAAAG GCCTCTTGCA GACTTCCCTA CAGCAGCAAA GGGAGCGCGT AGAACTCTTG
    GCGCAACAAA ACGAGACTCT TCTCCAGAGG CTCAGAGAAC AAGCTCAGAG TCAAGAAGCT
    GAGGCTTCAC GTGCCTCTCG GATGGCAGAA CTCGAACAGC GTTTGGCTGA GCAAGTAGAG
    AGTTCGCGAT TTGAAAGAGA GAAGCTGGTA GACATCCAGC AGCAGCTGAC AGGGAGTCTC
    AGAGCCTTGG AGAAGGAGAA TCAGGAGGCG CAAACTGCTG TGAAGAGCTT AAGGGAAGAA
    GAAGGGCTTC TGCAAGGGCA TCTGGAGAGT GAGAAGCTTG CTAGAGATGA GGCAGTGAGG
    AAAACTGAGG AACAGAGGCT AGCCATGGAG GCTCTGAGGG TGGACAATGC GAGTATGAAG
    GCGCAGGTGG AGGTGGAGCG GCAGAAGGTT GCTGAGCTGA AGGCAGTGCA GAGCGCTAGT
    GACAACACTG AGCTGCAAAG TCTGCTCAAA GTGGCGCATG AAGATCGAGA CAAGCTGGAG
    ATGACCTGCA CAGAGCTACG ACAAGAGCTG CAGCAGGTGG AGGCAGACAG ACAGCAGCTT
    CTGGAGAAGG TGGAGAAACA GGAACAAGAT CATAAAGCCA ACACAAATTC TCTCGAGGAA
    AAGAGCATAG ATGCTGAGAA CCAAATCAAG GACCTGAAAG AGACCATCTT CGAGTTGGAG
    GACCAGGTGG AGCAGCAACG GGCTGTTAGA CTGCACACCA ACCAGACCAT CCTGGACCTG
    GAGAATCAAA TCAAGAAGCT GGAAGACAAC AAAACTGAAC TGGAGAAGCA GATTAAGGTC
    GTGAATAAAC AAATGAAGGA TGAAACGGAG GAGTGGCGCC GTTTCCAGGC TGACCTCCAG
    ACAGCAGTAG TGGTTGCCAA TGACATAAAG GTGGAGGCTC AGCAGGAGCT GCGGACGCTG
    CGCAGACGTC TGCAGGAGGA GCAGGAGCAC AGCGCGAGAC TGGCTTCAGA GCTGGAGCAG
    ATCCAAGGCA CCAGGTCATG GAATGAAGAT GCCAGCCTCT CCGACTCAGA CGGCAGTCCA
    CACTGGAGCC GAATGACCAT TTCCCAGAAT GCACCTGTGG GTTTCCTAAC CAATGGCAAT
    GGCAGTGGAA GCAGTGTGTC CTCAGAGCCA GAAGCCACGG TCAAATCTCT CATCAAATCA
    TTTGACACCG CAACGCCGAA TGGATCTAGC AGCTCAGTAC AGATACACAC ATCTCCCAGA
    AGCCCCCTGA GTGGCATACC AGTCCGTACA GCTCCCGCTG CTGCCGTCTC ACCCATTCAG
    AGGCACTCTG GAATCAAGCC ACTGTCGAAG ACCCTCGAGA GAAGAATTGC CTTTGGAGAT
    TTCCCACACA ATGGTTCATG TGATGAACTG AAGCCCTCGA CTCTTATGAG GAAGAGCCCG
    TCTCTAGAGT CTGTGATTAA AACTCCAGCT CACTTCAACG GCCGCACTGC ATCGTTTACT
    TACAACAGAG CCAACAGCAA ACTCAGTGTG GAAAGAACAG ACCCTCTGTC CGCTCTGGCG
    CGGGAGTATG GTGGATCTAA GAGGAACGCT CTACTGAAGT GGTGCCAGAA GAAAACAGAG
    GGCTATCCGA ATATAGACGT GACGAACTTC AGCAGCAGCT GGAGCGACGG CCTAGCGTTC
    TGCGCTCTCT TACACACGTA TCTGCCTGCA CACATCCCCT ACCAGGAGCT CATCAGCCAA
    GACAAGGGGC GAAATCTAAC TCTGGCTTTT CAAGCAGCCG AAAGCATTGG TATCAAGCCC
    TCTCTGGACT TCGAGGAATT GATGCGCACC GATCGACCCG ACTGGCAAAG CGTCATGCAA
    TACGTGTCTC AGATTTACAA ATACTTCGAG ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.