SPECC1 cDNA ORF clone, Bos taurus(cattle)

The following SPECC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPECC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa10172 XM_005220461.4
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa10172 XM_015468078.1
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa46585 XM_010816100.3
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa46585 XM_010824089.2
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa227108 XM_024981169.1
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa227109 XM_024981170.1
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa227106 XM_024981167.1
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa227107 XM_024981168.1
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa10170 XM_015468072.1
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa10170 XM_002695819.5
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa10171 XM_015468077.1
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa10171 XM_005220460.4
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46582 XM_015468074.1
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46582 XM_010816096.2
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46582 XM_015458759.1
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46582 XM_015468075.1
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46582 XM_015468076.1
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46582 XM_015458758.2
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46582 XM_010816097.3
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46582 XM_015468073.1
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa46582 XM_015458758.1 Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa10170 XM_002695819.4 Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46582 XM_010816097.2 Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa10171 XM_005220460.3 Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46585 XM_010816100.2 Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa10172 XM_005220461.3 Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa10172
    Clone ID Related Accession (Same CDS sequence) XM_005220461.3 , XM_015468078.1 , XM_005220461.4
    Accession Version XM_005220461.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2373bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220461.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GCTCCATGGT CTCTGTCAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220518.1
    CDS485..2857
    Translation

    Target ORF information:

    RefSeq Version XM_005220461.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220461.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GCTCCATGGT CTCTGTCAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10172
    Clone ID Related Accession (Same CDS sequence) XM_005220461.3 , XM_015468078.1 , XM_005220461.4
    Accession Version XM_015468078.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2373bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GCTCCATGGT CTCTGTCAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323564.1
    CDS485..2857
    Translation

    Target ORF information:

    RefSeq Version XM_015468078.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468078.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GCTCCATGGT CTCTGTCAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10172
    Clone ID Related Accession (Same CDS sequence) XM_005220461.3 , XM_015468078.1 , XM_005220461.4
    Accession Version XM_005220461.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2373bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220461.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GCTCCATGGT CTCTGTCAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220518.1
    CDS139..2511
    Translation

    Target ORF information:

    RefSeq Version XM_005220461.4
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220461.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GCTCCATGGT CTCTGTCAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46585
    Clone ID Related Accession (Same CDS sequence) XM_010824089.2 , XM_010816100.2 , XM_010816100.3
    Accession Version XM_010824089.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824089.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCAACC ACTCGGGGCG GCCCGAGGAT CCCGAAGCAG GGTCCGTGAC AGCTACCAAG 
    AGGACAGGCA TTCCTGCCCC TCGAGAACTT GCCGTCACTG TCTCAAGAGA CAGAACTGTG
    CCACGCGGTC CCTCCAACTC AAGGAAAGTG ACGACCAGCC CCACCTCTTC CAGCACGCCC
    ACCCCCACCA AGCACGTGCG GGCTGCTCCC CCAAGACCCC GGCCGGAGCA TGAAGGTGCA
    GAGAAGGCTG TGCTCGAGTC CCAGGTCCGG GAGCTCTTGG CAGAAGCTAA AGAGAAAGAC
    AGTGAGATCA ACAGGCTCCG AAGTGAACTC AAGAAATGGA TGGAGAAACG GACTCAGGAC
    AGTGAAGGCG GGGATGCTTT GGACTCCAGT GTGGACGGAG CATCAGTCTT GCCAGGTGAC
    TCGGAACCTT TGATCCGAGC CCTTGAGGAG AAGAACAAGA GCTTCCAGAA AGAGCTCGCA
    GAGCTCGAGG AAGAAAACCG GGCCTTGAAA GAGAAACTGA CTTATTTTGA GCATTCCCCA
    GATTCAGAGG GGGGAGCAAG TCACACCGGG GACAGCAGCT GCCCGACGTC CGTAACTCAG
    GAGTCCAGCT TCGGGAGCCC GACGGGGAAT GAGGCGTTGA GTGAAATTGA TGTGTACAGA
    AAAAACCCAC ATGAGGGTGC CCTGCGGACA TCAGGCTCTT CCAGCAGTGA TGTCACCAAG
    GCCTCCTTGT CCCCGGATGC CTCTGACTTC GAACACATCC CAGCAGACAC CCTGGGGCCC
    CTGTCCCCTG CTGGGAGCCT TGTCAAGAGC TCCAAGTGCT CGGCAGCCGG CAGCTCCCCA
    AACACAGCGA GCGAGCTGTC CTTGGCGTCC CTGGCAGAGA AGATCCACAG GATGGAGGAG
    AACCAGCACA GCACGGCTGA GGAGCTGCAG GCCACGCTGC AGGAGCTGTC TGACCAGCAG
    CAGATGGTGC AGGAGCTGAC GGCTGAAAAT GAGAAGCTGG CGGATGAGAA GACGCTCCTG
    GAAACGTCCT TCCATCAGCA CCGGGAGAGG GCGGAGCAGC TGAGCCAGGA GAATGAGAAG
    CTTATGACCC TCCTGCAGGA GCGTGTCAAG GGCGAGGAGC CTGGCGCCCA GGAAGGCAAG
    ATCCTGGAGC TGGAACAGAA GTGCACAGAG ATCCTGGAGC AGGGCCGCTT CGAGCGCGAG
    AAGCTGCTGA GCATCCAACA GCAGCTGACG TGCCGCCTGC GCGCCGTCGA GGAGGAGCAC
    CGCAGTGCTC TGGAGCTGGT CCGGCACCTG CAGGAGGTGA ATGGGGAGCT GACCACCCGC
    CTGGAGCTGG AGCAGCACAG CGGCGGCCCC ACAGCCGAGA TCCCAGCAGC GCGTCAGCTT
    CCCGTGGAAG GGCTCCAGGT AGAGAACGGG TCTTTGCAGG CCTCCCTGGA GAACGAGGAG
    CAGAAAACTG GAGGGGCCAG CCCCCTGGGG CCCATGGCTG AGGGCTGCGA CGTCCAGGAA
    ATGCTGACCG TGGCTCGAGC CGAGAAGGAC CAGCTGGAAC AGTCCTGCTC AGAGCTCAGG
    CAGGAGCTGC TGAAGGCACA TGGAGAGGTG AAGCGCGTTT CAAGCCTGCT GGCCAAGGTG
    GAAAAGGATT ACTCTTACCT AAAGGAGATC TGCGATCACC AGGCAGAGCA GCTGAGCAGA
    ACCAGCCTGA AGCTACAAGA GAAAGCATCA GAGAGTGACG CAGAGATCAA AGACATGAAG
    GAGACCATAT TCGAGCTGGA GGACCAGGTG GAGCAGCATC GCGCTGTCAA GTTGCACAAC
    AACCAGCTCA TCAGTGAGCT GGAAAGCAGC GTGATGAAGC TGGAGGAACA GAAGTCAGAC
    CTGGAGAGGC AGCTGAAGAC TCTGACGAAG CAGATAAAGG ACGAGACGGA AGAGTGGCGG
    CGCTTCCAGG CTGACCTGCA GACGGCGGTA GTGGTGGCCA ATGACATCAA GGTTGAGGCC
    CAGCAGGAGC TGCGCGCTGT CAAGCGGAGG CTGCTGGAGG AGGAAGAGAA GAACGCCCGG
    CTGCAGAAGG CACTGGGGGA CGCACAGGCC CGCGGAAGAC CAGTGAAGGA AGAGCCCGAG
    CCCTCAGAGG CGGACGCTCC TGGCCGGTGG CCCAGCGCCT ATGTGAACAG GACTCCTCCA
    ACACCTTCAG AGTCTGTGAC CACTGTAAAG TCCCTCATCA AGTCATTTGA CTTGGGACGA
    CCAGGTGGAG CTGGGCAGAA TATTTCTGTC CACAAGACCC CCCGGAGTCC CCTGAGTGGG
    ATCCCTGTGA GGACGCCCCC AGCTGCTGCT GTTTCTCCAA TGCAGAGGCA TTCTGCCTTC
    AGCAGTGCTC GGCCGGCCAG CAAAGGAGAG ATTCAACGCT TGGATCTCCC AGACCTTCCC
    CTCTCAGATC TCCTGAAGGG AAGAGCTGAG GACCTGAAGC CAAACCCTTA TCTCCGTAAG
    AGCCCCTCTC TCGAGTCCCT GAGCAGACCC CCTTCTCTGG GCTTCGGGGG CTCCAGGCTG
    CTGGGCACCT CCCCTGCGGG CTGGAAGCCG CAGAGCAAAC TCAGTGTGGA AAGAAGAGAC
    CCCCTGGCGG CCCTGGCCCG GGAATACGGC GGCTCCAAGC GCAACGCTCT CCTCAGGTGG
    TGTCAGAGGA AGACAGAGGG CTACGCGAAC ATTGATATCA CCAATTTCAG CAGCAGCTGG
    AGTGATGGCT TGGCCTTTTG TGCTCTCCTC CACACCTACC TGCCCGCCCA CATCCCATAC
    CAGGAGCTGA ACAGCCAGGA GAAAAAAAGA AATCTCTTGT TGGCATTTGA AGCAGCTGAA
    AGTGTAGGCA TCAAACCCAG CCTGGAGCTC AGTGAGTTGC TGCACACAGA CCGGCCTGAC
    TGGCAGAGCG TGATGCAGTA TGTGGCCCAG ATCTACAAGT ACTTCGAGAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010822391.1
    CDS182..3115
    Translation

    Target ORF information:

    RefSeq Version XM_010824089.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824089.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCAACC ACTCGGGGCG GCCCGAGGAT CCCGAAGCAG GGTCCGTGAC AGCTACCAAG 
    AGGACAGGCA TTCCTGCCCC TCGAGAACTT GCCGTCACTG TCTCAAGAGA CAGAACTGTG
    CCACGCGGTC CCTCCAACTC AAGGAAAGTG ACGACCAGCC CCACCTCTTC CAGCACGCCC
    ACCCCCACCA AGCACGTGCG GGCTGCTCCC CCAAGACCCC GGCCGGAGCA TGAAGGTGCA
    GAGAAGGCTG TGCTCGAGTC CCAGGTCCGG GAGCTCTTGG CAGAAGCTAA AGAGAAAGAC
    AGTGAGATCA ACAGGCTCCG AAGTGAACTC AAGAAATGGA TGGAGAAACG GACTCAGGAC
    AGTGAAGGCG GGGATGCTTT GGACTCCAGT GTGGACGGAG CATCAGTCTT GCCAGGTGAC
    TCGGAACCTT TGATCCGAGC CCTTGAGGAG AAGAACAAGA GCTTCCAGAA AGAGCTCGCA
    GAGCTCGAGG AAGAAAACCG GGCCTTGAAA GAGAAACTGA CTTATTTTGA GCATTCCCCA
    GATTCAGAGG GGGGAGCAAG TCACACCGGG GACAGCAGCT GCCCGACGTC CGTAACTCAG
    GAGTCCAGCT TCGGGAGCCC GACGGGGAAT GAGGCGTTGA GTGAAATTGA TGTGTACAGA
    AAAAACCCAC ATGAGGGTGC CCTGCGGACA TCAGGCTCTT CCAGCAGTGA TGTCACCAAG
    GCCTCCTTGT CCCCGGATGC CTCTGACTTC GAACACATCC CAGCAGACAC CCTGGGGCCC
    CTGTCCCCTG CTGGGAGCCT TGTCAAGAGC TCCAAGTGCT CGGCAGCCGG CAGCTCCCCA
    AACACAGCGA GCGAGCTGTC CTTGGCGTCC CTGGCAGAGA AGATCCACAG GATGGAGGAG
    AACCAGCACA GCACGGCTGA GGAGCTGCAG GCCACGCTGC AGGAGCTGTC TGACCAGCAG
    CAGATGGTGC AGGAGCTGAC GGCTGAAAAT GAGAAGCTGG CGGATGAGAA GACGCTCCTG
    GAAACGTCCT TCCATCAGCA CCGGGAGAGG GCGGAGCAGC TGAGCCAGGA GAATGAGAAG
    CTTATGACCC TCCTGCAGGA GCGTGTCAAG GGCGAGGAGC CTGGCGCCCA GGAAGGCAAG
    ATCCTGGAGC TGGAACAGAA GTGCACAGAG ATCCTGGAGC AGGGCCGCTT CGAGCGCGAG
    AAGCTGCTGA GCATCCAACA GCAGCTGACG TGCCGCCTGC GCGCCGTCGA GGAGGAGCAC
    CGCAGTGCTC TGGAGCTGGT CCGGCACCTG CAGGAGGTGA ATGGGGAGCT GACCACCCGC
    CTGGAGCTGG AGCAGCACAG CGGCGGCCCC ACAGCCGAGA TCCCAGCAGC GCGTCAGCTT
    CCCGTGGAAG GGCTCCAGGT AGAGAACGGG TCTTTGCAGG CCTCCCTGGA GAACGAGGAG
    CAGAAAACTG GAGGGGCCAG CCCCCTGGGG CCCATGGCTG AGGGCTGCGA CGTCCAGGAA
    ATGCTGACCG TGGCTCGAGC CGAGAAGGAC CAGCTGGAAC AGTCCTGCTC AGAGCTCAGG
    CAGGAGCTGC TGAAGGCACA TGGAGAGGTG AAGCGCGTTT CAAGCCTGCT GGCCAAGGTG
    GAAAAGGATT ACTCTTACCT AAAGGAGATC TGCGATCACC AGGCAGAGCA GCTGAGCAGA
    ACCAGCCTGA AGCTACAAGA GAAAGCATCA GAGAGTGACG CAGAGATCAA AGACATGAAG
    GAGACCATAT TCGAGCTGGA GGACCAGGTG GAGCAGCATC GCGCTGTCAA GTTGCACAAC
    AACCAGCTCA TCAGTGAGCT GGAAAGCAGC GTGATGAAGC TGGAGGAACA GAAGTCAGAC
    CTGGAGAGGC AGCTGAAGAC TCTGACGAAG CAGATAAAGG ACGAGACGGA AGAGTGGCGG
    CGCTTCCAGG CTGACCTGCA GACGGCGGTA GTGGTGGCCA ATGACATCAA GGTTGAGGCC
    CAGCAGGAGC TGCGCGCTGT CAAGCGGAGG CTGCTGGAGG AGGAAGAGAA GAACGCCCGG
    CTGCAGAAGG CACTGGGGGA CGCACAGGCC CGCGGAAGAC CAGTGAAGGA AGAGCCCGAG
    CCCTCAGAGG CGGACGCTCC TGGCCGGTGG CCCAGCGCCT ATGTGAACAG GACTCCTCCA
    ACACCTTCAG AGTCTGTGAC CACTGTAAAG TCCCTCATCA AGTCATTTGA CTTGGGACGA
    CCAGGTGGAG CTGGGCAGAA TATTTCTGTC CACAAGACCC CCCGGAGTCC CCTGAGTGGG
    ATCCCTGTGA GGACGCCCCC AGCTGCTGCT GTTTCTCCAA TGCAGAGGCA TTCTGCCTTC
    AGCAGTGCTC GGCCGGCCAG CAAAGGAGAG ATTCAACGCT TGGATCTCCC AGACCTTCCC
    CTCTCAGATC TCCTGAAGGG AAGAGCTGAG GACCTGAAGC CAAACCCTTA TCTCCGTAAG
    AGCCCCTCTC TCGAGTCCCT GAGCAGACCC CCTTCTCTGG GCTTCGGGGG CTCCAGGCTG
    CTGGGCACCT CCCCTGCGGG CTGGAAGCCG CAGAGCAAAC TCAGTGTGGA AAGAAGAGAC
    CCCCTGGCGG CCCTGGCCCG GGAATACGGC GGCTCCAAGC GCAACGCTCT CCTCAGGTGG
    TGTCAGAGGA AGACAGAGGG CTACGCGAAC ATTGATATCA CCAATTTCAG CAGCAGCTGG
    AGTGATGGCT TGGCCTTTTG TGCTCTCCTC CACACCTACC TGCCCGCCCA CATCCCATAC
    CAGGAGCTGA ACAGCCAGGA GAAAAAAAGA AATCTCTTGT TGGCATTTGA AGCAGCTGAA
    AGTGTAGGCA TCAAACCCAG CCTGGAGCTC AGTGAGTTGC TGCACACAGA CCGGCCTGAC
    TGGCAGAGCG TGATGCAGTA TGTGGCCCAG ATCTACAAGT ACTTCGAGAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46585
    Clone ID Related Accession (Same CDS sequence) XM_010824089.2 , XM_010816100.2 , XM_010816100.3
    Accession Version XM_010816100.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816100.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCAACC ACTCGGGGCG GCCCGAGGAT CCCGAAGCAG GGTCCGTGAC AGCTACCAAG 
    AGGACAGGCA TTCCTGCCCC TCGAGAACTT GCCGTCACTG TCTCAAGAGA CAGAACTGTG
    CCACGCGGTC CCTCCAACTC AAGGAAAGTG ACGACCAGCC CCACCTCTTC CAGCACGCCC
    ACCCCCACCA AGCACGTGCG GGCTGCTCCC CCAAGACCCC GGCCGGAGCA TGAAGGTGCA
    GAGAAGGCTG TGCTCGAGTC CCAGGTCCGG GAGCTCTTGG CAGAAGCTAA AGAGAAAGAC
    AGTGAGATCA ACAGGCTCCG AAGTGAACTC AAGAAATGGA TGGAGAAACG GACTCAGGAC
    AGTGAAGGCG GGGATGCTTT GGACTCCAGT GTGGACGGAG CATCAGTCTT GCCAGGTGAC
    TCGGAACCTT TGATCCGAGC CCTTGAGGAG AAGAACAAGA GCTTCCAGAA AGAGCTCGCA
    GAGCTCGAGG AAGAAAACCG GGCCTTGAAA GAGAAACTGA CTTATTTTGA GCATTCCCCA
    GATTCAGAGG GGGGAGCAAG TCACACCGGG GACAGCAGCT GCCCGACGTC CGTAACTCAG
    GAGTCCAGCT TCGGGAGCCC GACGGGGAAT GAGGCGTTGA GTGAAATTGA TGTGTACAGA
    AAAAACCCAC ATGAGGGTGC CCTGCGGACA TCAGGCTCTT CCAGCAGTGA TGTCACCAAG
    GCCTCCTTGT CCCCGGATGC CTCTGACTTC GAACACATCC CAGCAGACAC CCTGGGGCCC
    CTGTCCCCTG CTGGGAGCCT TGTCAAGAGC TCCAAGTGCT CGGCAGCCGG CAGCTCCCCA
    AACACAGCGA GCGAGCTGTC CTTGGCGTCC CTGGCAGAGA AGATCCACAG GATGGAGGAG
    AACCAGCACA GCACGGCTGA GGAGCTGCAG GCCACGCTGC AGGAGCTGTC TGACCAGCAG
    CAGATGGTGC AGGAGCTGAC GGCTGAAAAT GAGAAGCTGG CGGATGAGAA GACGCTCCTG
    GAAACGTCCT TCCATCAGCA CCGGGAGAGG GCGGAGCAGC TGAGCCAGGA GAATGAGAAG
    CTTATGACCC TCCTGCAGGA GCGTGTCAAG GGCGAGGAGC CTGGCGCCCA GGAAGGCAAG
    ATCCTGGAGC TGGAACAGAA GTGCACAGAG ATCCTGGAGC AGGGCCGCTT CGAGCGCGAG
    AAGCTGCTGA GCATCCAACA GCAGCTGACG TGCCGCCTGC GCGCCGTCGA GGAGGAGCAC
    CGCAGTGCTC TGGAGCTGGT CCGGCACCTG CAGGAGGTGA ATGGGGAGCT GACCACCCGC
    CTGGAGCTGG AGCAGCACAG CGGCGGCCCC ACAGCCGAGA TCCCAGCAGC GCGTCAGCTT
    CCCGTGGAAG GGCTCCAGGT AGAGAACGGG TCTTTGCAGG CCTCCCTGGA GAACGAGGAG
    CAGAAAACTG GAGGGGCCAG CCCCCTGGGG CCCATGGCTG AGGGCTGCGA CGTCCAGGAA
    ATGCTGACCG TGGCTCGAGC CGAGAAGGAC CAGCTGGAAC AGTCCTGCTC AGAGCTCAGG
    CAGGAGCTGC TGAAGGCACA TGGAGAGGTG AAGCGCGTTT CAAGCCTGCT GGCCAAGGTG
    GAAAAGGATT ACTCTTACCT AAAGGAGATC TGCGATCACC AGGCAGAGCA GCTGAGCAGA
    ACCAGCCTGA AGCTACAAGA GAAAGCATCA GAGAGTGACG CAGAGATCAA AGACATGAAG
    GAGACCATAT TCGAGCTGGA GGACCAGGTG GAGCAGCATC GCGCTGTCAA GTTGCACAAC
    AACCAGCTCA TCAGTGAGCT GGAAAGCAGC GTGATGAAGC TGGAGGAACA GAAGTCAGAC
    CTGGAGAGGC AGCTGAAGAC TCTGACGAAG CAGATAAAGG ACGAGACGGA AGAGTGGCGG
    CGCTTCCAGG CTGACCTGCA GACGGCGGTA GTGGTGGCCA ATGACATCAA GGTTGAGGCC
    CAGCAGGAGC TGCGCGCTGT CAAGCGGAGG CTGCTGGAGG AGGAAGAGAA GAACGCCCGG
    CTGCAGAAGG CACTGGGGGA CGCACAGGCC CGCGGAAGAC CAGTGAAGGA AGAGCCCGAG
    CCCTCAGAGG CGGACGCTCC TGGCCGGTGG CCCAGCGCCT ATGTGAACAG GACTCCTCCA
    ACACCTTCAG AGTCTGTGAC CACTGTAAAG TCCCTCATCA AGTCATTTGA CTTGGGACGA
    CCAGGTGGAG CTGGGCAGAA TATTTCTGTC CACAAGACCC CCCGGAGTCC CCTGAGTGGG
    ATCCCTGTGA GGACGCCCCC AGCTGCTGCT GTTTCTCCAA TGCAGAGGCA TTCTGCCTTC
    AGCAGTGCTC GGCCGGCCAG CAAAGGAGAG ATTCAACGCT TGGATCTCCC AGACCTTCCC
    CTCTCAGATC TCCTGAAGGG AAGAGCTGAG GACCTGAAGC CAAACCCTTA TCTCCGTAAG
    AGCCCCTCTC TCGAGTCCCT GAGCAGACCC CCTTCTCTGG GCTTCGGGGG CTCCAGGCTG
    CTGGGCACCT CCCCTGCGGG CTGGAAGCCG CAGAGCAAAC TCAGTGTGGA AAGAAGAGAC
    CCCCTGGCGG CCCTGGCCCG GGAATACGGC GGCTCCAAGC GCAACGCTCT CCTCAGGTGG
    TGTCAGAGGA AGACAGAGGG CTACGCGAAC ATTGATATCA CCAATTTCAG CAGCAGCTGG
    AGTGATGGCT TGGCCTTTTG TGCTCTCCTC CACACCTACC TGCCCGCCCA CATCCCATAC
    CAGGAGCTGA ACAGCCAGGA GAAAAAAAGA AATCTCTTGT TGGCATTTGA AGCAGCTGAA
    AGTGTAGGCA TCAAACCCAG CCTGGAGCTC AGTGAGTTGC TGCACACAGA CCGGCCTGAC
    TGGCAGAGCG TGATGCAGTA TGTGGCCCAG ATCTACAAGT ACTTCGAGAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814402.1
    CDS182..3115
    Translation

    Target ORF information:

    RefSeq Version XM_010816100.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816100.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCAACC ACTCGGGGCG GCCCGAGGAT CCCGAAGCAG GGTCCGTGAC AGCTACCAAG 
    AGGACAGGCA TTCCTGCCCC TCGAGAACTT GCCGTCACTG TCTCAAGAGA CAGAACTGTG
    CCACGCGGTC CCTCCAACTC AAGGAAAGTG ACGACCAGCC CCACCTCTTC CAGCACGCCC
    ACCCCCACCA AGCACGTGCG GGCTGCTCCC CCAAGACCCC GGCCGGAGCA TGAAGGTGCA
    GAGAAGGCTG TGCTCGAGTC CCAGGTCCGG GAGCTCTTGG CAGAAGCTAA AGAGAAAGAC
    AGTGAGATCA ACAGGCTCCG AAGTGAACTC AAGAAATGGA TGGAGAAACG GACTCAGGAC
    AGTGAAGGCG GGGATGCTTT GGACTCCAGT GTGGACGGAG CATCAGTCTT GCCAGGTGAC
    TCGGAACCTT TGATCCGAGC CCTTGAGGAG AAGAACAAGA GCTTCCAGAA AGAGCTCGCA
    GAGCTCGAGG AAGAAAACCG GGCCTTGAAA GAGAAACTGA CTTATTTTGA GCATTCCCCA
    GATTCAGAGG GGGGAGCAAG TCACACCGGG GACAGCAGCT GCCCGACGTC CGTAACTCAG
    GAGTCCAGCT TCGGGAGCCC GACGGGGAAT GAGGCGTTGA GTGAAATTGA TGTGTACAGA
    AAAAACCCAC ATGAGGGTGC CCTGCGGACA TCAGGCTCTT CCAGCAGTGA TGTCACCAAG
    GCCTCCTTGT CCCCGGATGC CTCTGACTTC GAACACATCC CAGCAGACAC CCTGGGGCCC
    CTGTCCCCTG CTGGGAGCCT TGTCAAGAGC TCCAAGTGCT CGGCAGCCGG CAGCTCCCCA
    AACACAGCGA GCGAGCTGTC CTTGGCGTCC CTGGCAGAGA AGATCCACAG GATGGAGGAG
    AACCAGCACA GCACGGCTGA GGAGCTGCAG GCCACGCTGC AGGAGCTGTC TGACCAGCAG
    CAGATGGTGC AGGAGCTGAC GGCTGAAAAT GAGAAGCTGG CGGATGAGAA GACGCTCCTG
    GAAACGTCCT TCCATCAGCA CCGGGAGAGG GCGGAGCAGC TGAGCCAGGA GAATGAGAAG
    CTTATGACCC TCCTGCAGGA GCGTGTCAAG GGCGAGGAGC CTGGCGCCCA GGAAGGCAAG
    ATCCTGGAGC TGGAACAGAA GTGCACAGAG ATCCTGGAGC AGGGCCGCTT CGAGCGCGAG
    AAGCTGCTGA GCATCCAACA GCAGCTGACG TGCCGCCTGC GCGCCGTCGA GGAGGAGCAC
    CGCAGTGCTC TGGAGCTGGT CCGGCACCTG CAGGAGGTGA ATGGGGAGCT GACCACCCGC
    CTGGAGCTGG AGCAGCACAG CGGCGGCCCC ACAGCCGAGA TCCCAGCAGC GCGTCAGCTT
    CCCGTGGAAG GGCTCCAGGT AGAGAACGGG TCTTTGCAGG CCTCCCTGGA GAACGAGGAG
    CAGAAAACTG GAGGGGCCAG CCCCCTGGGG CCCATGGCTG AGGGCTGCGA CGTCCAGGAA
    ATGCTGACCG TGGCTCGAGC CGAGAAGGAC CAGCTGGAAC AGTCCTGCTC AGAGCTCAGG
    CAGGAGCTGC TGAAGGCACA TGGAGAGGTG AAGCGCGTTT CAAGCCTGCT GGCCAAGGTG
    GAAAAGGATT ACTCTTACCT AAAGGAGATC TGCGATCACC AGGCAGAGCA GCTGAGCAGA
    ACCAGCCTGA AGCTACAAGA GAAAGCATCA GAGAGTGACG CAGAGATCAA AGACATGAAG
    GAGACCATAT TCGAGCTGGA GGACCAGGTG GAGCAGCATC GCGCTGTCAA GTTGCACAAC
    AACCAGCTCA TCAGTGAGCT GGAAAGCAGC GTGATGAAGC TGGAGGAACA GAAGTCAGAC
    CTGGAGAGGC AGCTGAAGAC TCTGACGAAG CAGATAAAGG ACGAGACGGA AGAGTGGCGG
    CGCTTCCAGG CTGACCTGCA GACGGCGGTA GTGGTGGCCA ATGACATCAA GGTTGAGGCC
    CAGCAGGAGC TGCGCGCTGT CAAGCGGAGG CTGCTGGAGG AGGAAGAGAA GAACGCCCGG
    CTGCAGAAGG CACTGGGGGA CGCACAGGCC CGCGGAAGAC CAGTGAAGGA AGAGCCCGAG
    CCCTCAGAGG CGGACGCTCC TGGCCGGTGG CCCAGCGCCT ATGTGAACAG GACTCCTCCA
    ACACCTTCAG AGTCTGTGAC CACTGTAAAG TCCCTCATCA AGTCATTTGA CTTGGGACGA
    CCAGGTGGAG CTGGGCAGAA TATTTCTGTC CACAAGACCC CCCGGAGTCC CCTGAGTGGG
    ATCCCTGTGA GGACGCCCCC AGCTGCTGCT GTTTCTCCAA TGCAGAGGCA TTCTGCCTTC
    AGCAGTGCTC GGCCGGCCAG CAAAGGAGAG ATTCAACGCT TGGATCTCCC AGACCTTCCC
    CTCTCAGATC TCCTGAAGGG AAGAGCTGAG GACCTGAAGC CAAACCCTTA TCTCCGTAAG
    AGCCCCTCTC TCGAGTCCCT GAGCAGACCC CCTTCTCTGG GCTTCGGGGG CTCCAGGCTG
    CTGGGCACCT CCCCTGCGGG CTGGAAGCCG CAGAGCAAAC TCAGTGTGGA AAGAAGAGAC
    CCCCTGGCGG CCCTGGCCCG GGAATACGGC GGCTCCAAGC GCAACGCTCT CCTCAGGTGG
    TGTCAGAGGA AGACAGAGGG CTACGCGAAC ATTGATATCA CCAATTTCAG CAGCAGCTGG
    AGTGATGGCT TGGCCTTTTG TGCTCTCCTC CACACCTACC TGCCCGCCCA CATCCCATAC
    CAGGAGCTGA ACAGCCAGGA GAAAAAAAGA AATCTCTTGT TGGCATTTGA AGCAGCTGAA
    AGTGTAGGCA TCAAACCCAG CCTGGAGCTC AGTGAGTTGC TGCACACAGA CCGGCCTGAC
    TGGCAGAGCG TGATGCAGTA TGTGGCCCAG ATCTACAAGT ACTTCGAGAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46585
    Clone ID Related Accession (Same CDS sequence) XM_010824089.2 , XM_010816100.2 , XM_010816100.3
    Accession Version XM_010816100.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816100.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCAACC ACTCGGGGCG GCCCGAGGAT CCCGAAGCAG GGTCCGTGAC AGCTACCAAG 
    AGGACAGGCA TTCCTGCCCC TCGAGAACTT GCCGTCACTG TCTCAAGAGA CAGAACTGTG
    CCACGCGGTC CCTCCAACTC AAGGAAAGTG ACGACCAGCC CCACCTCTTC CAGCACGCCC
    ACCCCCACCA AGCACGTGCG GGCTGCTCCC CCAAGACCCC GGCCGGAGCA TGAAGGTGCA
    GAGAAGGCTG TGCTCGAGTC CCAGGTCCGG GAGCTCTTGG CAGAAGCTAA AGAGAAAGAC
    AGTGAGATCA ACAGGCTCCG AAGTGAACTC AAGAAATGGA TGGAGAAACG GACTCAGGAC
    AGTGAAGGCG GGGATGCTTT GGACTCCAGT GTGGACGGAG CATCAGTCTT GCCAGGTGAC
    TCGGAACCTT TGATCCGAGC CCTTGAGGAG AAGAACAAGA GCTTCCAGAA AGAGCTCGCA
    GAGCTCGAGG AAGAAAACCG GGCCTTGAAA GAGAAACTGA CTTATTTTGA GCATTCCCCA
    GATTCAGAGG GGGGAGCAAG TCACACCGGG GACAGCAGCT GCCCGACGTC CGTAACTCAG
    GAGTCCAGCT TCGGGAGCCC GACGGGGAAT GAGGCGTTGA GTGAAATTGA TGTGTACAGA
    AAAAACCCAC ATGAGGGTGC CCTGCGGACA TCAGGCTCTT CCAGCAGTGA TGTCACCAAG
    GCCTCCTTGT CCCCGGATGC CTCTGACTTC GAACACATCC CAGCAGACAC CCTGGGGCCC
    CTGTCCCCTG CTGGGAGCCT TGTCAAGAGC TCCAAGTGCT CGGCAGCCGG CAGCTCCCCA
    AACACAGCGA GCGAGCTGTC CTTGGCGTCC CTGGCAGAGA AGATCCACAG GATGGAGGAG
    AACCAGCACA GCACGGCTGA GGAGCTGCAG GCCACGCTGC AGGAGCTGTC TGACCAGCAG
    CAGATGGTGC AGGAGCTGAC GGCTGAAAAT GAGAAGCTGG CGGATGAGAA GACGCTCCTG
    GAAACGTCCT TCCATCAGCA CCGGGAGAGG GCGGAGCAGC TGAGCCAGGA GAATGAGAAG
    CTTATGACCC TCCTGCAGGA GCGTGTCAAG GGCGAGGAGC CTGGCGCCCA GGAAGGCAAG
    ATCCTGGAGC TGGAACAGAA GTGCACAGAG ATCCTGGAGC AGGGCCGCTT CGAGCGCGAG
    AAGCTGCTGA GCATCCAACA GCAGCTGACG TGCCGCCTGC GCGCCGTCGA GGAGGAGCAC
    CGCAGTGCTC TGGAGCTGGT CCGGCACCTG CAGGAGGTGA ATGGGGAGCT GACCACCCGC
    CTGGAGCTGG AGCAGCACAG CGGCGGCCCC ACAGCCGAGA TCCCAGCAGC GCGTCAGCTT
    CCCGTGGAAG GGCTCCAGGT AGAGAACGGG TCTTTGCAGG CCTCCCTGGA GAACGAGGAG
    CAGAAAACTG GAGGGGCCAG CCCCCTGGGG CCCATGGCTG AGGGCTGCGA CGTCCAGGAA
    ATGCTGACCG TGGCTCGAGC CGAGAAGGAC CAGCTGGAAC AGTCCTGCTC AGAGCTCAGG
    CAGGAGCTGC TGAAGGCACA TGGAGAGGTG AAGCGCGTTT CAAGCCTGCT GGCCAAGGTG
    GAAAAGGATT ACTCTTACCT AAAGGAGATC TGCGATCACC AGGCAGAGCA GCTGAGCAGA
    ACCAGCCTGA AGCTACAAGA GAAAGCATCA GAGAGTGACG CAGAGATCAA AGACATGAAG
    GAGACCATAT TCGAGCTGGA GGACCAGGTG GAGCAGCATC GCGCTGTCAA GTTGCACAAC
    AACCAGCTCA TCAGTGAGCT GGAAAGCAGC GTGATGAAGC TGGAGGAACA GAAGTCAGAC
    CTGGAGAGGC AGCTGAAGAC TCTGACGAAG CAGATAAAGG ACGAGACGGA AGAGTGGCGG
    CGCTTCCAGG CTGACCTGCA GACGGCGGTA GTGGTGGCCA ATGACATCAA GGTTGAGGCC
    CAGCAGGAGC TGCGCGCTGT CAAGCGGAGG CTGCTGGAGG AGGAAGAGAA GAACGCCCGG
    CTGCAGAAGG CACTGGGGGA CGCACAGGCC CGCGGAAGAC CAGTGAAGGA AGAGCCCGAG
    CCCTCAGAGG CGGACGCTCC TGGCCGGTGG CCCAGCGCCT ATGTGAACAG GACTCCTCCA
    ACACCTTCAG AGTCTGTGAC CACTGTAAAG TCCCTCATCA AGTCATTTGA CTTGGGACGA
    CCAGGTGGAG CTGGGCAGAA TATTTCTGTC CACAAGACCC CCCGGAGTCC CCTGAGTGGG
    ATCCCTGTGA GGACGCCCCC AGCTGCTGCT GTTTCTCCAA TGCAGAGGCA TTCTGCCTTC
    AGCAGTGCTC GGCCGGCCAG CAAAGGAGAG ATTCAACGCT TGGATCTCCC AGACCTTCCC
    CTCTCAGATC TCCTGAAGGG AAGAGCTGAG GACCTGAAGC CAAACCCTTA TCTCCGTAAG
    AGCCCCTCTC TCGAGTCCCT GAGCAGACCC CCTTCTCTGG GCTTCGGGGG CTCCAGGCTG
    CTGGGCACCT CCCCTGCGGG CTGGAAGCCG CAGAGCAAAC TCAGTGTGGA AAGAAGAGAC
    CCCCTGGCGG CCCTGGCCCG GGAATACGGC GGCTCCAAGC GCAACGCTCT CCTCAGGTGG
    TGTCAGAGGA AGACAGAGGG CTACGCGAAC ATTGATATCA CCAATTTCAG CAGCAGCTGG
    AGTGATGGCT TGGCCTTTTG TGCTCTCCTC CACACCTACC TGCCCGCCCA CATCCCATAC
    CAGGAGCTGA ACAGCCAGGA GAAAAAAAGA AATCTCTTGT TGGCATTTGA AGCAGCTGAA
    AGTGTAGGCA TCAAACCCAG CCTGGAGCTC AGTGAGTTGC TGCACACAGA CCGGCCTGAC
    TGGCAGAGCG TGATGCAGTA TGTGGCCCAG ATCTACAAGT ACTTCGAGAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814402.1
    CDS205..3138
    Translation

    Target ORF information:

    RefSeq Version XM_010816100.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816100.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGCAACC ACTCGGGGCG GCCCGAGGAT CCCGAAGCAG GGTCCGTGAC AGCTACCAAG 
    AGGACAGGCA TTCCTGCCCC TCGAGAACTT GCCGTCACTG TCTCAAGAGA CAGAACTGTG
    CCACGCGGTC CCTCCAACTC AAGGAAAGTG ACGACCAGCC CCACCTCTTC CAGCACGCCC
    ACCCCCACCA AGCACGTGCG GGCTGCTCCC CCAAGACCCC GGCCGGAGCA TGAAGGTGCA
    GAGAAGGCTG TGCTCGAGTC CCAGGTCCGG GAGCTCTTGG CAGAAGCTAA AGAGAAAGAC
    AGTGAGATCA ACAGGCTCCG AAGTGAACTC AAGAAATGGA TGGAGAAACG GACTCAGGAC
    AGTGAAGGCG GGGATGCTTT GGACTCCAGT GTGGACGGAG CATCAGTCTT GCCAGGTGAC
    TCGGAACCTT TGATCCGAGC CCTTGAGGAG AAGAACAAGA GCTTCCAGAA AGAGCTCGCA
    GAGCTCGAGG AAGAAAACCG GGCCTTGAAA GAGAAACTGA CTTATTTTGA GCATTCCCCA
    GATTCAGAGG GGGGAGCAAG TCACACCGGG GACAGCAGCT GCCCGACGTC CGTAACTCAG
    GAGTCCAGCT TCGGGAGCCC GACGGGGAAT GAGGCGTTGA GTGAAATTGA TGTGTACAGA
    AAAAACCCAC ATGAGGGTGC CCTGCGGACA TCAGGCTCTT CCAGCAGTGA TGTCACCAAG
    GCCTCCTTGT CCCCGGATGC CTCTGACTTC GAACACATCC CAGCAGACAC CCTGGGGCCC
    CTGTCCCCTG CTGGGAGCCT TGTCAAGAGC TCCAAGTGCT CGGCAGCCGG CAGCTCCCCA
    AACACAGCGA GCGAGCTGTC CTTGGCGTCC CTGGCAGAGA AGATCCACAG GATGGAGGAG
    AACCAGCACA GCACGGCTGA GGAGCTGCAG GCCACGCTGC AGGAGCTGTC TGACCAGCAG
    CAGATGGTGC AGGAGCTGAC GGCTGAAAAT GAGAAGCTGG CGGATGAGAA GACGCTCCTG
    GAAACGTCCT TCCATCAGCA CCGGGAGAGG GCGGAGCAGC TGAGCCAGGA GAATGAGAAG
    CTTATGACCC TCCTGCAGGA GCGTGTCAAG GGCGAGGAGC CTGGCGCCCA GGAAGGCAAG
    ATCCTGGAGC TGGAACAGAA GTGCACAGAG ATCCTGGAGC AGGGCCGCTT CGAGCGCGAG
    AAGCTGCTGA GCATCCAACA GCAGCTGACG TGCCGCCTGC GCGCCGTCGA GGAGGAGCAC
    CGCAGTGCTC TGGAGCTGGT CCGGCACCTG CAGGAGGTGA ATGGGGAGCT GACCACCCGC
    CTGGAGCTGG AGCAGCACAG CGGCGGCCCC ACAGCCGAGA TCCCAGCAGC GCGTCAGCTT
    CCCGTGGAAG GGCTCCAGGT AGAGAACGGG TCTTTGCAGG CCTCCCTGGA GAACGAGGAG
    CAGAAAACTG GAGGGGCCAG CCCCCTGGGG CCCATGGCTG AGGGCTGCGA CGTCCAGGAA
    ATGCTGACCG TGGCTCGAGC CGAGAAGGAC CAGCTGGAAC AGTCCTGCTC AGAGCTCAGG
    CAGGAGCTGC TGAAGGCACA TGGAGAGGTG AAGCGCGTTT CAAGCCTGCT GGCCAAGGTG
    GAAAAGGATT ACTCTTACCT AAAGGAGATC TGCGATCACC AGGCAGAGCA GCTGAGCAGA
    ACCAGCCTGA AGCTACAAGA GAAAGCATCA GAGAGTGACG CAGAGATCAA AGACATGAAG
    GAGACCATAT TCGAGCTGGA GGACCAGGTG GAGCAGCATC GCGCTGTCAA GTTGCACAAC
    AACCAGCTCA TCAGTGAGCT GGAAAGCAGC GTGATGAAGC TGGAGGAACA GAAGTCAGAC
    CTGGAGAGGC AGCTGAAGAC TCTGACGAAG CAGATAAAGG ACGAGACGGA AGAGTGGCGG
    CGCTTCCAGG CTGACCTGCA GACGGCGGTA GTGGTGGCCA ATGACATCAA GGTTGAGGCC
    CAGCAGGAGC TGCGCGCTGT CAAGCGGAGG CTGCTGGAGG AGGAAGAGAA GAACGCCCGG
    CTGCAGAAGG CACTGGGGGA CGCACAGGCC CGCGGAAGAC CAGTGAAGGA AGAGCCCGAG
    CCCTCAGAGG CGGACGCTCC TGGCCGGTGG CCCAGCGCCT ATGTGAACAG GACTCCTCCA
    ACACCTTCAG AGTCTGTGAC CACTGTAAAG TCCCTCATCA AGTCATTTGA CTTGGGACGA
    CCAGGTGGAG CTGGGCAGAA TATTTCTGTC CACAAGACCC CCCGGAGTCC CCTGAGTGGG
    ATCCCTGTGA GGACGCCCCC AGCTGCTGCT GTTTCTCCAA TGCAGAGGCA TTCTGCCTTC
    AGCAGTGCTC GGCCGGCCAG CAAAGGAGAG ATTCAACGCT TGGATCTCCC AGACCTTCCC
    CTCTCAGATC TCCTGAAGGG AAGAGCTGAG GACCTGAAGC CAAACCCTTA TCTCCGTAAG
    AGCCCCTCTC TCGAGTCCCT GAGCAGACCC CCTTCTCTGG GCTTCGGGGG CTCCAGGCTG
    CTGGGCACCT CCCCTGCGGG CTGGAAGCCG CAGAGCAAAC TCAGTGTGGA AAGAAGAGAC
    CCCCTGGCGG CCCTGGCCCG GGAATACGGC GGCTCCAAGC GCAACGCTCT CCTCAGGTGG
    TGTCAGAGGA AGACAGAGGG CTACGCGAAC ATTGATATCA CCAATTTCAG CAGCAGCTGG
    AGTGATGGCT TGGCCTTTTG TGCTCTCCTC CACACCTACC TGCCCGCCCA CATCCCATAC
    CAGGAGCTGA ACAGCCAGGA GAAAAAAAGA AATCTCTTGT TGGCATTTGA AGCAGCTGAA
    AGTGTAGGCA TCAAACCCAG CCTGGAGCTC AGTGAGTTGC TGCACACAGA CCGGCCTGAC
    TGGCAGAGCG TGATGCAGTA TGTGGCCCAG ATCTACAAGT ACTTCGAGAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227108
    Clone ID Related Accession (Same CDS sequence) XM_024981169.1
    Accession Version XM_024981169.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2817bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGCAACC ACTCGGGGCG GCCCGAGGAT CCCGAAGCAG GGTCCGTGAC AGCTACCAAG 
    AGGACAGGCA TTCCTGCCCC TCGAGAACTT GCCGTCACTG TCTCAAGAGA CAGAACTGTG
    CCACGCGGTC CCTCCAACTC AAGGAAAGTG ACGACCAGCC CCACCTCTTC CAGCACGCCC
    ACCCCCACCA AGCACGTGCG GGCTGCTCCC CCAAGACCCC GGCCGGAGCA TGAAGGTGCA
    GAGAAGGCTG TGCTCGAGTC CCAGGTCCGG GAGCTCTTGG CAGAAGCTAA AGAGAAAGAC
    AGTGAGATCA ACAGGCTCCG AAGTGAACTC AAGAAATGGA TGGAGAAACG GACTCAGGAC
    AGTGAAGGCG GGGATGCTTT GGACTCCAGT GTGGACGGAG CATCAGTCTT GCCAGGTGAC
    TCGGAACCTT TGATCCGAGC CCTTGAGGAG AAGAACAAGA GCTTCCAGAA AGAGCTCGCA
    GAGCTCGAGG AAGAAAACCG GGCCTTGAAA GAGAAACTGA CTTATTTTGA GCATTCCCCA
    GATTCAGAGG GGGGAGCAAG TCACACCGGG GACAGCAGCT GCCCGACGTC CGTAACTCAG
    GAGTCCAGCT TCGGGAGCCC GACGGGGAAT GAGGCGTTGA GTGAAATTGA TGTGTACAGA
    AAAAACCCAC ATGAGGGTGC CCTGCGGACA TCAGGCTCTT CCAGCAGTGA TGTCACCAAG
    GCCTCCTTGT CCCCGGATGC CTCTGACTTC GAACACATCC CAGCAGACAC CCTGGGGCCC
    CTGTCCCCTG CTGGGAGCCT TGTCAAGAGC TCCAAGTGCT CGGCAGCCGG CAGCTCCCCA
    AACACAGCGA GCGAGCTGTC CTTGGCGTCC CTGGCAGAGA AGATCCACAG GATGGAGGAG
    AACCAGCACA GCACGGCTGA GGAGCTGCAG GCCACGCTGC AGGAGCTGTC TGACCAGCAG
    CAGATGGTGC AGGAGCTGAC GGCTGAAAAT GAGAAGCTGG CGGATGAGAA GACGCTCCTG
    GAAACGTCCT TCCATCAGCA CCGGGAGAGG GCGGAGCAGC TGAGCCAGGA GAATGAGAAG
    CTTATGACCC TCCTGCAGGA GCGTGTCAAG GGCGAGGAGC CTGGCGCCCA GGAAGGCAAG
    ATCCTGGAGC TGGAACAGAA GTGCACAGAG ATCCTGGAGC AGGGCCGCTT CGAGCGCGAG
    AAGCTGCTGA GCATCCAACA GCAGCTGACG TGCCGCCTGC GCGCCGTCGA GGAGGAGCAC
    CGCAGTGCTC TGGAGCTGGT CCGGCACCTG CAGGAGGTGA ATGGGGAGCT GACCACCCGC
    CTGGAGCTGG AGCAGCACAG CGGCGGCCCC ACAGCCGAGA TCCCAGCAGC GCGTCAGCTT
    CCCGTGGAAG GGCTCCAGGT AGAGAACGGG TCTTTGCAGG CCTCCCTGGA GAACGAGGAG
    CAGAAAACTG GAGGGGCCAG CCCCCTGGGG CCCATGGCTG AGGGCTGCGA CGTCCAGGAA
    ATGCTGACCG TGGCTCGAGC CGAGAAGGAC CAGCTGGAAC AGTCCTGCTC AGAGCTCAGG
    CAGGAGCTGC TGAAGGCACA TGGAGAGGTG AAGCGCGTTT CAAGCCTGCT GGCCAAGGTG
    GAAAAGGATT ACTCTTACCT AAAGGAGATC TGCGATCACC AGGCAGAGCA GCTGAGCAGA
    ACCAGCCTGA AGCTACAAGA GAAAGCATCA GAGAGTGACG CAGAGATCAA AGACATGAAG
    GAGACCATAT TCGAGCTGGA GGACCAGGTG GAGCAGCATC GCGCTGTCAA GTTGCACAAC
    AACCAGCTCA TCAGTGAGCT GGAAAGCAGC GTGATGAAGC TGGAGGAACA GAAGTCAGAC
    CTGGAGAGGC AGCTGAAGAC TCTGACGAAG CAGATAAAGG ACGAGACGGA AGAGTGGCGG
    CGCTTCCAGG CTGACCTGCA GACGGCGGTA GTGGTGGCCA ATGACATCAA GGTTGAGGCC
    CAGCAGGAGC TGCGCGCTGT CAAGCGGAGG CTGCTGGAGG AGGAAGAGAA GAACGCCCGG
    CTGCAGAAGG CACTGGGGGA CGCACAGGCC CGCGGAAGAC CAGTGAAGGA AGAGCCCGAG
    CCCTCAGAGG CGGACGCTCC TGGCCGGTGG CCCAGCGCCT ATGTGAACAG GACTCCTCCA
    ACACCTTCAG AGTCTGTGAC CACTGTAAAG TCCCTCATCA AGTCATTTGA CTTGGGACGA
    CCAGGTGGAG CTGGGCAGAA TATTTCTGTC CACAAGACCC CCCGGAGTCC CCTGAGTGGG
    ATCCCTGTGA GGACGCCCCC AGCTGCTGCT GTTTCTCCAA TGCAGAGGCA TTCTGCCTTC
    AGCAGTGCTC GGCCGGCCAG CAAAGGAGAG ATTCAACGCT TGGATCTCCC AGACCTTCCC
    CTCTCAGATC TCCTGAAGGG AAGAGCTGAG GACCTGAAGC CAAACCCTTA TCTCCGTAAG
    AGCCCCTCTC TCGAGTCCCT GAGCAGACCC CCTTCTCTGG GCTTCGGGGG CTCCAGGCTG
    CTGGGCACCT CCCCTGCGGG CTGGAAGCCG CAGAGCAAAC TCAGTGTGGA AAGAAGAGAC
    CCCCTGGCGG CCCTGGCCCG GGAATACGGC GGCTCCAAGC GCAACGCTCT CCTCAGGTGG
    TGTCAGAGGA AGACAGAGGG CTACGCGAAA AGAAATCTCT TGTTGGCATT TGAAGCAGCT
    GAAAGTGTAG GCATCAAACC CAGCCTGGAG CTCAGTGAGT TGCTGCACAC AGACCGGCCT
    GACTGGCAGA GCGTGATGCA GTATGTGGCC CAGATCTACA AGTACTTCGA GACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836937.1
    CDS165..2981
    Translation

    Target ORF information:

    RefSeq Version XM_024981169.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981169.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGCAACC ACTCGGGGCG GCCCGAGGAT CCCGAAGCAG GGTCCGTGAC AGCTACCAAG 
    AGGACAGGCA TTCCTGCCCC TCGAGAACTT GCCGTCACTG TCTCAAGAGA CAGAACTGTG
    CCACGCGGTC CCTCCAACTC AAGGAAAGTG ACGACCAGCC CCACCTCTTC CAGCACGCCC
    ACCCCCACCA AGCACGTGCG GGCTGCTCCC CCAAGACCCC GGCCGGAGCA TGAAGGTGCA
    GAGAAGGCTG TGCTCGAGTC CCAGGTCCGG GAGCTCTTGG CAGAAGCTAA AGAGAAAGAC
    AGTGAGATCA ACAGGCTCCG AAGTGAACTC AAGAAATGGA TGGAGAAACG GACTCAGGAC
    AGTGAAGGCG GGGATGCTTT GGACTCCAGT GTGGACGGAG CATCAGTCTT GCCAGGTGAC
    TCGGAACCTT TGATCCGAGC CCTTGAGGAG AAGAACAAGA GCTTCCAGAA AGAGCTCGCA
    GAGCTCGAGG AAGAAAACCG GGCCTTGAAA GAGAAACTGA CTTATTTTGA GCATTCCCCA
    GATTCAGAGG GGGGAGCAAG TCACACCGGG GACAGCAGCT GCCCGACGTC CGTAACTCAG
    GAGTCCAGCT TCGGGAGCCC GACGGGGAAT GAGGCGTTGA GTGAAATTGA TGTGTACAGA
    AAAAACCCAC ATGAGGGTGC CCTGCGGACA TCAGGCTCTT CCAGCAGTGA TGTCACCAAG
    GCCTCCTTGT CCCCGGATGC CTCTGACTTC GAACACATCC CAGCAGACAC CCTGGGGCCC
    CTGTCCCCTG CTGGGAGCCT TGTCAAGAGC TCCAAGTGCT CGGCAGCCGG CAGCTCCCCA
    AACACAGCGA GCGAGCTGTC CTTGGCGTCC CTGGCAGAGA AGATCCACAG GATGGAGGAG
    AACCAGCACA GCACGGCTGA GGAGCTGCAG GCCACGCTGC AGGAGCTGTC TGACCAGCAG
    CAGATGGTGC AGGAGCTGAC GGCTGAAAAT GAGAAGCTGG CGGATGAGAA GACGCTCCTG
    GAAACGTCCT TCCATCAGCA CCGGGAGAGG GCGGAGCAGC TGAGCCAGGA GAATGAGAAG
    CTTATGACCC TCCTGCAGGA GCGTGTCAAG GGCGAGGAGC CTGGCGCCCA GGAAGGCAAG
    ATCCTGGAGC TGGAACAGAA GTGCACAGAG ATCCTGGAGC AGGGCCGCTT CGAGCGCGAG
    AAGCTGCTGA GCATCCAACA GCAGCTGACG TGCCGCCTGC GCGCCGTCGA GGAGGAGCAC
    CGCAGTGCTC TGGAGCTGGT CCGGCACCTG CAGGAGGTGA ATGGGGAGCT GACCACCCGC
    CTGGAGCTGG AGCAGCACAG CGGCGGCCCC ACAGCCGAGA TCCCAGCAGC GCGTCAGCTT
    CCCGTGGAAG GGCTCCAGGT AGAGAACGGG TCTTTGCAGG CCTCCCTGGA GAACGAGGAG
    CAGAAAACTG GAGGGGCCAG CCCCCTGGGG CCCATGGCTG AGGGCTGCGA CGTCCAGGAA
    ATGCTGACCG TGGCTCGAGC CGAGAAGGAC CAGCTGGAAC AGTCCTGCTC AGAGCTCAGG
    CAGGAGCTGC TGAAGGCACA TGGAGAGGTG AAGCGCGTTT CAAGCCTGCT GGCCAAGGTG
    GAAAAGGATT ACTCTTACCT AAAGGAGATC TGCGATCACC AGGCAGAGCA GCTGAGCAGA
    ACCAGCCTGA AGCTACAAGA GAAAGCATCA GAGAGTGACG CAGAGATCAA AGACATGAAG
    GAGACCATAT TCGAGCTGGA GGACCAGGTG GAGCAGCATC GCGCTGTCAA GTTGCACAAC
    AACCAGCTCA TCAGTGAGCT GGAAAGCAGC GTGATGAAGC TGGAGGAACA GAAGTCAGAC
    CTGGAGAGGC AGCTGAAGAC TCTGACGAAG CAGATAAAGG ACGAGACGGA AGAGTGGCGG
    CGCTTCCAGG CTGACCTGCA GACGGCGGTA GTGGTGGCCA ATGACATCAA GGTTGAGGCC
    CAGCAGGAGC TGCGCGCTGT CAAGCGGAGG CTGCTGGAGG AGGAAGAGAA GAACGCCCGG
    CTGCAGAAGG CACTGGGGGA CGCACAGGCC CGCGGAAGAC CAGTGAAGGA AGAGCCCGAG
    CCCTCAGAGG CGGACGCTCC TGGCCGGTGG CCCAGCGCCT ATGTGAACAG GACTCCTCCA
    ACACCTTCAG AGTCTGTGAC CACTGTAAAG TCCCTCATCA AGTCATTTGA CTTGGGACGA
    CCAGGTGGAG CTGGGCAGAA TATTTCTGTC CACAAGACCC CCCGGAGTCC CCTGAGTGGG
    ATCCCTGTGA GGACGCCCCC AGCTGCTGCT GTTTCTCCAA TGCAGAGGCA TTCTGCCTTC
    AGCAGTGCTC GGCCGGCCAG CAAAGGAGAG ATTCAACGCT TGGATCTCCC AGACCTTCCC
    CTCTCAGATC TCCTGAAGGG AAGAGCTGAG GACCTGAAGC CAAACCCTTA TCTCCGTAAG
    AGCCCCTCTC TCGAGTCCCT GAGCAGACCC CCTTCTCTGG GCTTCGGGGG CTCCAGGCTG
    CTGGGCACCT CCCCTGCGGG CTGGAAGCCG CAGAGCAAAC TCAGTGTGGA AAGAAGAGAC
    CCCCTGGCGG CCCTGGCCCG GGAATACGGC GGCTCCAAGC GCAACGCTCT CCTCAGGTGG
    TGTCAGAGGA AGACAGAGGG CTACGCGAAA AGAAATCTCT TGTTGGCATT TGAAGCAGCT
    GAAAGTGTAG GCATCAAACC CAGCCTGGAG CTCAGTGAGT TGCTGCACAC AGACCGGCCT
    GACTGGCAGA GCGTGATGCA GTATGTGGCC CAGATCTACA AGTACTTCGA GACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227109
    Clone ID Related Accession (Same CDS sequence) XM_024981170.1
    Accession Version XM_024981170.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2100bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGGCAACC ACTCGGGGCG GCCCGAGGAT CCCGAAGCAG GGTCCGTGAC AGCTACCAAG 
    AGGACAGGCA TTCCTGCCCC TCGAGAACTT GCCGTCACTG TCTCAAGAGA CAGAACTGTG
    CCACGCGGTC CCTCCAACTC AAGGAAAGTG ACGACCAGCC CCACCTCTTC CAGCACGCCC
    ACCCCCACCA AGCACGTGCG GGCTGCTCCC CCAAGACCCC GGCCGGAGCA TGAAGGTGCA
    GAGAAGGCTG TGCTCGAGTC CCAGGTCCGG GAGCTCTTGG CAGAAGCTAA AGAGAAAGAC
    AGTGAGATCA ACAGGCTCCG AAGTGAACTC AAGAAATGGA TGGAGAAACG GACTCAGGAC
    AGTGAAGGCG GGGATGCTTT GGACTCCAGT GTGGACGGAG CATCAGTCTT GCCAGGTGAC
    TCGGAACCTT TGATCCGAGC CCTTGAGGAG AAGAACAAGA GCTTCCAGAA AGAGCTCGCA
    GAGCTCGAGG AAGAAAACCG GGCCTTGAAA GAGAAACTGA CTTATTTTGA GCATTCCCCA
    GATTCAGAGG GGGGAGCAAG TCACACCGGG GACAGCAGCT GCCCGACGTC CGTAACTCAG
    GAGTCCAGCT TCGGGAGCCC GACGGGGAAT GAGGCGTTGA GTGAAATTGA TGTGTACAGA
    AAAAACCCAC ATGAGGGTGC CCTGCGGACA TCAGGCTCTT CCAGCAGTGA TGTCACCAAG
    GCCTCCTTGT CCCCGGATGC CTCTGACTTC GAACACATCC CAGCAGACAC CCTGGGGCCC
    CTGTCCCCTG CTGGGAGCCT TGTCAAGAGC TCCAAGTGCT CGGCAGCCGG CAGCTCCCCA
    AACACAGCGA GCGAGCTGTC CTTGGCGTCC CTGGCAGAGA AGATCCACAG GATGGAGGAG
    AACCAGCACA GCACGGCTGA GGAGCTGCAG GCCACGCTGC AGGAGCTGTC TGACCAGCAG
    CAGATGGTGC AGGAGCTGAC GGCTGAAAAT GAGAAGCTGG CGGATGAGAA GACGCTCCTG
    GAAACGTCCT TCCATCAGCA CCGGGAGAGG GCGGAGCAGC TGAGCCAGGA GAATGAGAAG
    CTTATGACCC TCCTGCAGGA GCGTGTCAAG GGCGAGGAGC CTGGCGCCCA GGAAGGCAAG
    ATCCTGGAGC TGGAACAGAA GTGCACAGAG ATCCTGGAGC AGGGCCGCTT CGAGCGCGAG
    AAGCTGCTGA GCATCCAACA GCAGCTGACG TGCCGCCTGC GCGCCGTCGA GGAGGAGCAC
    CGCAGTGCTC TGGAGCTGGT CCGGCACCTG CAGGAGGTGA ATGGGGAGCT GACCACCCGC
    CTGGAGCTGG AGCAGCACAG CGGCGGCCCC ACAGCCGAGA TCCCAGCAGC GCGTCAGCTT
    CCCGTGGAAG GGCTCCAGGT AGAGAACGGG TCTTTGCAGG CCTCCCTGGA GAACGAGGAG
    CAGAAAACTG GAGGGGCCAG CCCCCTGGGG CCCATGGCTG AGGGCTGCGA CGTCCAGGAA
    ATGCTGACCG TGGCTCGAGC CGAGAAGGAC CAGCTGGAAC AGTCCTGCTC AGAGCTCAGG
    CAGGAGCTGC TGAAGGCACA TGGAGAGGTG AAGCGCGTTT CAAGCCTGCT GGCCAAGGTG
    GAAAAGGATT ACTCTTACCT AAAGGAGATC TGCGATCACC AGGCAGAGCA GCTGAGCAGA
    ACCAGCCTGA AGCTACAAGA GAAAGCATCA GAGAGTGACG CAGAGATCAA AGACATGAAG
    GAGACCATAT TCGAGCTGGA GGACCAGGTG GAGCAGCATC GCGCTGTCAA GTTGCACAAC
    AACCAGCTCA TCAGTGAGCT GGAAAGCAGC GTGATGAAGC TGGAGGAACA GAAGTCAGAC
    CTGGAGAGGC AGCTGAAGAC TCTGACGAAG CAGATAAAGG ACGAGACGGA AGAGTGGCGG
    CGCTTCCAGG CTGACCTGCA GACGGCGGTA GTGGTGGCCA ATGACATCAA GGTTGAGGCC
    CAGCAGGAGC TGCGCGCTGT CAAGCGGAGG CTGCTGGAGG AGGAAGAGAA GAACGCCCGG
    CTGCAGAAGG CACTGGGGGA CGCACAGGCC CGCGGAAGCT CCATGGTCTC TGTCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836938.1
    CDS160..2259
    Translation

    Target ORF information:

    RefSeq Version XM_024981170.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981170.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGGCAACC ACTCGGGGCG GCCCGAGGAT CCCGAAGCAG GGTCCGTGAC AGCTACCAAG 
    AGGACAGGCA TTCCTGCCCC TCGAGAACTT GCCGTCACTG TCTCAAGAGA CAGAACTGTG
    CCACGCGGTC CCTCCAACTC AAGGAAAGTG ACGACCAGCC CCACCTCTTC CAGCACGCCC
    ACCCCCACCA AGCACGTGCG GGCTGCTCCC CCAAGACCCC GGCCGGAGCA TGAAGGTGCA
    GAGAAGGCTG TGCTCGAGTC CCAGGTCCGG GAGCTCTTGG CAGAAGCTAA AGAGAAAGAC
    AGTGAGATCA ACAGGCTCCG AAGTGAACTC AAGAAATGGA TGGAGAAACG GACTCAGGAC
    AGTGAAGGCG GGGATGCTTT GGACTCCAGT GTGGACGGAG CATCAGTCTT GCCAGGTGAC
    TCGGAACCTT TGATCCGAGC CCTTGAGGAG AAGAACAAGA GCTTCCAGAA AGAGCTCGCA
    GAGCTCGAGG AAGAAAACCG GGCCTTGAAA GAGAAACTGA CTTATTTTGA GCATTCCCCA
    GATTCAGAGG GGGGAGCAAG TCACACCGGG GACAGCAGCT GCCCGACGTC CGTAACTCAG
    GAGTCCAGCT TCGGGAGCCC GACGGGGAAT GAGGCGTTGA GTGAAATTGA TGTGTACAGA
    AAAAACCCAC ATGAGGGTGC CCTGCGGACA TCAGGCTCTT CCAGCAGTGA TGTCACCAAG
    GCCTCCTTGT CCCCGGATGC CTCTGACTTC GAACACATCC CAGCAGACAC CCTGGGGCCC
    CTGTCCCCTG CTGGGAGCCT TGTCAAGAGC TCCAAGTGCT CGGCAGCCGG CAGCTCCCCA
    AACACAGCGA GCGAGCTGTC CTTGGCGTCC CTGGCAGAGA AGATCCACAG GATGGAGGAG
    AACCAGCACA GCACGGCTGA GGAGCTGCAG GCCACGCTGC AGGAGCTGTC TGACCAGCAG
    CAGATGGTGC AGGAGCTGAC GGCTGAAAAT GAGAAGCTGG CGGATGAGAA GACGCTCCTG
    GAAACGTCCT TCCATCAGCA CCGGGAGAGG GCGGAGCAGC TGAGCCAGGA GAATGAGAAG
    CTTATGACCC TCCTGCAGGA GCGTGTCAAG GGCGAGGAGC CTGGCGCCCA GGAAGGCAAG
    ATCCTGGAGC TGGAACAGAA GTGCACAGAG ATCCTGGAGC AGGGCCGCTT CGAGCGCGAG
    AAGCTGCTGA GCATCCAACA GCAGCTGACG TGCCGCCTGC GCGCCGTCGA GGAGGAGCAC
    CGCAGTGCTC TGGAGCTGGT CCGGCACCTG CAGGAGGTGA ATGGGGAGCT GACCACCCGC
    CTGGAGCTGG AGCAGCACAG CGGCGGCCCC ACAGCCGAGA TCCCAGCAGC GCGTCAGCTT
    CCCGTGGAAG GGCTCCAGGT AGAGAACGGG TCTTTGCAGG CCTCCCTGGA GAACGAGGAG
    CAGAAAACTG GAGGGGCCAG CCCCCTGGGG CCCATGGCTG AGGGCTGCGA CGTCCAGGAA
    ATGCTGACCG TGGCTCGAGC CGAGAAGGAC CAGCTGGAAC AGTCCTGCTC AGAGCTCAGG
    CAGGAGCTGC TGAAGGCACA TGGAGAGGTG AAGCGCGTTT CAAGCCTGCT GGCCAAGGTG
    GAAAAGGATT ACTCTTACCT AAAGGAGATC TGCGATCACC AGGCAGAGCA GCTGAGCAGA
    ACCAGCCTGA AGCTACAAGA GAAAGCATCA GAGAGTGACG CAGAGATCAA AGACATGAAG
    GAGACCATAT TCGAGCTGGA GGACCAGGTG GAGCAGCATC GCGCTGTCAA GTTGCACAAC
    AACCAGCTCA TCAGTGAGCT GGAAAGCAGC GTGATGAAGC TGGAGGAACA GAAGTCAGAC
    CTGGAGAGGC AGCTGAAGAC TCTGACGAAG CAGATAAAGG ACGAGACGGA AGAGTGGCGG
    CGCTTCCAGG CTGACCTGCA GACGGCGGTA GTGGTGGCCA ATGACATCAA GGTTGAGGCC
    CAGCAGGAGC TGCGCGCTGT CAAGCGGAGG CTGCTGGAGG AGGAAGAGAA GAACGCCCGG
    CTGCAGAAGG CACTGGGGGA CGCACAGGCC CGCGGAAGCT CCATGGTCTC TGTCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227106
    Clone ID Related Accession (Same CDS sequence) XM_024981167.1
    Accession Version XM_024981167.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GACCAGTGAA GGAAGAGCCC GAGCCCTCAG AGGCGGACGC TCCTGGCCGG
    TGGCCCAGCG CCTATGTGAA CAGGACTCCT CCAACACCTT CAGAGTCTGT GACCACTGTA
    AAGTCCCTCA TCAAGTCATT TGACTTGGGA CGACCAGGTG GAGCTGGGCA GAATATTTCT
    GTCCACAAGA CCCCCCGGAG TCCCCTGAGT GGGATCCCTG TGAGGACGCC CCCAGCTGCT
    GCTGTTTCTC CAATGCAGAG GCATTCTGCC TTCAGCAGTG CTCGGCCGGC CAGCAAAGGA
    GAGATTCAAC GCTTGGATCT CCCAGACCTT CCCCTCTCAG ATCTCCTGAA GGGAAGAGCT
    GAGGACCTGA AGCCAAACCC TTATCTCCGT AAGAGCCCCT CTCTCGAGTC CCTGAGCAGA
    CCCCCTTCTC TGGGCTTCGG GGGCTCCAGG CTGCTGGGCA CCTCCCCTGC GGGCTGGAAG
    CCGCAGAGCA AACTCAGTGT GGAAAGAAGA GACCCCCTGG CGGCCCTGGC CCGGGAATAC
    GGCGGCTCCA AGCGCAACGC TCTCCTCAGG TGGTGTCAGA GGAAGACAGA GGGCTACGCG
    AACATTGATA TCACCAATTT CAGCAGCAGC TGGAGTGATG GCTTGGCCTT TTGTGCTCTC
    CTCCACACCT ACCTGCCCGC CCACATCCCA TACCAGGAGC TGAACAGCCA GGAGAAAAAA
    AGAAATCTCT TGTTGGCATT TGAAGCAGCT GAAAGTGTAG GCATCAAACC CAGCCTGCAA
    GAGTTTGCAA GGAGCCTGGA TCCCACTACA GGCGACCACA TGGCATATGG ATCCTTCTGC
    CCCCTTTCAG CCCAACGCCT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836935.1
    CDS139..3342
    Translation

    Target ORF information:

    RefSeq Version XM_024981167.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981167.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GACCAGTGAA GGAAGAGCCC GAGCCCTCAG AGGCGGACGC TCCTGGCCGG
    TGGCCCAGCG CCTATGTGAA CAGGACTCCT CCAACACCTT CAGAGTCTGT GACCACTGTA
    AAGTCCCTCA TCAAGTCATT TGACTTGGGA CGACCAGGTG GAGCTGGGCA GAATATTTCT
    GTCCACAAGA CCCCCCGGAG TCCCCTGAGT GGGATCCCTG TGAGGACGCC CCCAGCTGCT
    GCTGTTTCTC CAATGCAGAG GCATTCTGCC TTCAGCAGTG CTCGGCCGGC CAGCAAAGGA
    GAGATTCAAC GCTTGGATCT CCCAGACCTT CCCCTCTCAG ATCTCCTGAA GGGAAGAGCT
    GAGGACCTGA AGCCAAACCC TTATCTCCGT AAGAGCCCCT CTCTCGAGTC CCTGAGCAGA
    CCCCCTTCTC TGGGCTTCGG GGGCTCCAGG CTGCTGGGCA CCTCCCCTGC GGGCTGGAAG
    CCGCAGAGCA AACTCAGTGT GGAAAGAAGA GACCCCCTGG CGGCCCTGGC CCGGGAATAC
    GGCGGCTCCA AGCGCAACGC TCTCCTCAGG TGGTGTCAGA GGAAGACAGA GGGCTACGCG
    AACATTGATA TCACCAATTT CAGCAGCAGC TGGAGTGATG GCTTGGCCTT TTGTGCTCTC
    CTCCACACCT ACCTGCCCGC CCACATCCCA TACCAGGAGC TGAACAGCCA GGAGAAAAAA
    AGAAATCTCT TGTTGGCATT TGAAGCAGCT GAAAGTGTAG GCATCAAACC CAGCCTGCAA
    GAGTTTGCAA GGAGCCTGGA TCCCACTACA GGCGACCACA TGGCATATGG ATCCTTCTGC
    CCCCTTTCAG CCCAACGCCT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227107
    Clone ID Related Accession (Same CDS sequence) XM_024981168.1
    Accession Version XM_024981168.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3087bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GACCAGTGAA GGAAGAGCCC GAGCCCTCAG AGGCGGACGC TCCTGGCCGG
    TGGCCCAGCG CCTATGTGAA CAGGACTCCT CCAACACCTT CAGAGTCTGT GACCACTGTA
    AAGTCCCTCA TCAAGTCATT TGACTTGGGA CGACCAGGTG GAGCTGGGCA GAATATTTCT
    GTCCACAAGA CCCCCCGGAG TCCCCTGAGT GGGATCCCTG TGAGGACGCC CCCAGCTGCT
    GCTGTTTCTC CAATGCAGAG GCATTCTGCC TTCAGCAGTG CTCGGCCGGC CAGCAAAGGA
    GAGATTCAAC GCTTGGATCT CCCAGACCTT CCCCTCTCAG ATCTCCTGAA GGGAAGAGCT
    GAGGACCTGA AGCCAAACCC TTATCTCCGT AAGAGCCCCT CTCTCGAGTC CCTGAGCAGA
    CCCCCTTCTC TGGGCTTCGG GGGCTCCAGG CTGCTGGGCA CCTCCCCTGC GGGCTGGAAG
    CCGCAGAGCA AACTCAGTGT GGAAAGAAGA GACCCCCTGG CGGCCCTGGC CCGGGAATAC
    GGCGGCTCCA AGCGCAACGC TCTCCTCAGG TGGTGTCAGA GGAAGACAGA GGGCTACGCG
    AAAAGAAATC TCTTGTTGGC ATTTGAAGCA GCTGAAAGTG TAGGCATCAA ACCCAGCCTG
    CAAGAGTTTG CAAGGAGCCT GGATCCCACT ACAGGCGACC ACATGGCATA TGGATCCTTC
    TGCCCCCTTT CAGCCCAACG CCTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836936.1
    CDS139..3225
    Translation

    Target ORF information:

    RefSeq Version XM_024981168.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981168.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GACCAGTGAA GGAAGAGCCC GAGCCCTCAG AGGCGGACGC TCCTGGCCGG
    TGGCCCAGCG CCTATGTGAA CAGGACTCCT CCAACACCTT CAGAGTCTGT GACCACTGTA
    AAGTCCCTCA TCAAGTCATT TGACTTGGGA CGACCAGGTG GAGCTGGGCA GAATATTTCT
    GTCCACAAGA CCCCCCGGAG TCCCCTGAGT GGGATCCCTG TGAGGACGCC CCCAGCTGCT
    GCTGTTTCTC CAATGCAGAG GCATTCTGCC TTCAGCAGTG CTCGGCCGGC CAGCAAAGGA
    GAGATTCAAC GCTTGGATCT CCCAGACCTT CCCCTCTCAG ATCTCCTGAA GGGAAGAGCT
    GAGGACCTGA AGCCAAACCC TTATCTCCGT AAGAGCCCCT CTCTCGAGTC CCTGAGCAGA
    CCCCCTTCTC TGGGCTTCGG GGGCTCCAGG CTGCTGGGCA CCTCCCCTGC GGGCTGGAAG
    CCGCAGAGCA AACTCAGTGT GGAAAGAAGA GACCCCCTGG CGGCCCTGGC CCGGGAATAC
    GGCGGCTCCA AGCGCAACGC TCTCCTCAGG TGGTGTCAGA GGAAGACAGA GGGCTACGCG
    AAAAGAAATC TCTTGTTGGC ATTTGAAGCA GCTGAAAGTG TAGGCATCAA ACCCAGCCTG
    CAAGAGTTTG CAAGGAGCCT GGATCCCACT ACAGGCGACC ACATGGCATA TGGATCCTTC
    TGCCCCCTTT CAGCCCAACG CCTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10170
    Clone ID Related Accession (Same CDS sequence) XM_002695819.4 , XM_015468072.1 , XM_002695819.5
    Accession Version XM_002695819.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002695819.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GACCAGTGAA GGAAGAGCCC GAGCCCTCAG AGGCGGACGC TCCTGGCCGG
    TGGCCCAGCG CCTATGTGAA CAGGACTCCT CCAACACCTT CAGAGTCTGT GACCACTGTA
    AAGTCCCTCA TCAAGTCATT TGACTTGGGA CGACCAGGTG GAGCTGGGCA GAATATTTCT
    GTCCACAAGA CCCCCCGGAG TCCCCTGAGT GGGATCCCTG TGAGGACGCC CCCAGCTGCT
    GCTGTTTCTC CAATGCAGAG GCATTCTGCC TTCAGCAGTG CTCGGCCGGC CAGCAAAGGA
    GAGATTCAAC GCTTGGATCT CCCAGACCTT CCCCTCTCAG ATCTCCTGAA GGGAAGAGCT
    GAGGACCTGA AGCCAAACCC TTATCTCCGT AAGAGCCCCT CTCTCGAGTC CCTGAGCAGA
    CCCCCTTCTC TGGGCTTCGG GGGCTCCAGG CTGCTGGGCA CCTCCCCTGC GGGCTGGAAG
    CCGCAGAGCA AACTCAGTGT GGAAAGAAGA GACCCCCTGG CGGCCCTGGC CCGGGAATAC
    GGCGGCTCCA AGCGCAACGC TCTCCTCAGG TGGTGTCAGA GGAAGACAGA GGGCTACGCG
    AACATTGATA TCACCAATTT CAGCAGCAGC TGGAGTGATG GCTTGGCCTT TTGTGCTCTC
    CTCCACACCT ACCTGCCCGC CCACATCCCA TACCAGGAGC TGAACAGCCA GGAGAAAAAA
    AGAAATCTCT TGTTGGCATT TGAAGCAGCT GAAAGTGTAG GCATCAAACC CAGCCTGGAG
    CTCAGTGAGT TGCTGCACAC AGACCGGCCT GACTGGCAGA GCGTGATGCA GTATGTGGCC
    CAGATCTACA AGTACTTCGA GACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002695865.2
    CDS488..3694
    Translation

    Target ORF information:

    RefSeq Version XM_002695819.4
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002695819.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GACCAGTGAA GGAAGAGCCC GAGCCCTCAG AGGCGGACGC TCCTGGCCGG
    TGGCCCAGCG CCTATGTGAA CAGGACTCCT CCAACACCTT CAGAGTCTGT GACCACTGTA
    AAGTCCCTCA TCAAGTCATT TGACTTGGGA CGACCAGGTG GAGCTGGGCA GAATATTTCT
    GTCCACAAGA CCCCCCGGAG TCCCCTGAGT GGGATCCCTG TGAGGACGCC CCCAGCTGCT
    GCTGTTTCTC CAATGCAGAG GCATTCTGCC TTCAGCAGTG CTCGGCCGGC CAGCAAAGGA
    GAGATTCAAC GCTTGGATCT CCCAGACCTT CCCCTCTCAG ATCTCCTGAA GGGAAGAGCT
    GAGGACCTGA AGCCAAACCC TTATCTCCGT AAGAGCCCCT CTCTCGAGTC CCTGAGCAGA
    CCCCCTTCTC TGGGCTTCGG GGGCTCCAGG CTGCTGGGCA CCTCCCCTGC GGGCTGGAAG
    CCGCAGAGCA AACTCAGTGT GGAAAGAAGA GACCCCCTGG CGGCCCTGGC CCGGGAATAC
    GGCGGCTCCA AGCGCAACGC TCTCCTCAGG TGGTGTCAGA GGAAGACAGA GGGCTACGCG
    AACATTGATA TCACCAATTT CAGCAGCAGC TGGAGTGATG GCTTGGCCTT TTGTGCTCTC
    CTCCACACCT ACCTGCCCGC CCACATCCCA TACCAGGAGC TGAACAGCCA GGAGAAAAAA
    AGAAATCTCT TGTTGGCATT TGAAGCAGCT GAAAGTGTAG GCATCAAACC CAGCCTGGAG
    CTCAGTGAGT TGCTGCACAC AGACCGGCCT GACTGGCAGA GCGTGATGCA GTATGTGGCC
    CAGATCTACA AGTACTTCGA GACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10170
    Clone ID Related Accession (Same CDS sequence) XM_002695819.4 , XM_015468072.1 , XM_002695819.5
    Accession Version XM_015468072.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GACCAGTGAA GGAAGAGCCC GAGCCCTCAG AGGCGGACGC TCCTGGCCGG
    TGGCCCAGCG CCTATGTGAA CAGGACTCCT CCAACACCTT CAGAGTCTGT GACCACTGTA
    AAGTCCCTCA TCAAGTCATT TGACTTGGGA CGACCAGGTG GAGCTGGGCA GAATATTTCT
    GTCCACAAGA CCCCCCGGAG TCCCCTGAGT GGGATCCCTG TGAGGACGCC CCCAGCTGCT
    GCTGTTTCTC CAATGCAGAG GCATTCTGCC TTCAGCAGTG CTCGGCCGGC CAGCAAAGGA
    GAGATTCAAC GCTTGGATCT CCCAGACCTT CCCCTCTCAG ATCTCCTGAA GGGAAGAGCT
    GAGGACCTGA AGCCAAACCC TTATCTCCGT AAGAGCCCCT CTCTCGAGTC CCTGAGCAGA
    CCCCCTTCTC TGGGCTTCGG GGGCTCCAGG CTGCTGGGCA CCTCCCCTGC GGGCTGGAAG
    CCGCAGAGCA AACTCAGTGT GGAAAGAAGA GACCCCCTGG CGGCCCTGGC CCGGGAATAC
    GGCGGCTCCA AGCGCAACGC TCTCCTCAGG TGGTGTCAGA GGAAGACAGA GGGCTACGCG
    AACATTGATA TCACCAATTT CAGCAGCAGC TGGAGTGATG GCTTGGCCTT TTGTGCTCTC
    CTCCACACCT ACCTGCCCGC CCACATCCCA TACCAGGAGC TGAACAGCCA GGAGAAAAAA
    AGAAATCTCT TGTTGGCATT TGAAGCAGCT GAAAGTGTAG GCATCAAACC CAGCCTGGAG
    CTCAGTGAGT TGCTGCACAC AGACCGGCCT GACTGGCAGA GCGTGATGCA GTATGTGGCC
    CAGATCTACA AGTACTTCGA GACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323558.1
    CDS488..3694
    Translation

    Target ORF information:

    RefSeq Version XM_015468072.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468072.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GACCAGTGAA GGAAGAGCCC GAGCCCTCAG AGGCGGACGC TCCTGGCCGG
    TGGCCCAGCG CCTATGTGAA CAGGACTCCT CCAACACCTT CAGAGTCTGT GACCACTGTA
    AAGTCCCTCA TCAAGTCATT TGACTTGGGA CGACCAGGTG GAGCTGGGCA GAATATTTCT
    GTCCACAAGA CCCCCCGGAG TCCCCTGAGT GGGATCCCTG TGAGGACGCC CCCAGCTGCT
    GCTGTTTCTC CAATGCAGAG GCATTCTGCC TTCAGCAGTG CTCGGCCGGC CAGCAAAGGA
    GAGATTCAAC GCTTGGATCT CCCAGACCTT CCCCTCTCAG ATCTCCTGAA GGGAAGAGCT
    GAGGACCTGA AGCCAAACCC TTATCTCCGT AAGAGCCCCT CTCTCGAGTC CCTGAGCAGA
    CCCCCTTCTC TGGGCTTCGG GGGCTCCAGG CTGCTGGGCA CCTCCCCTGC GGGCTGGAAG
    CCGCAGAGCA AACTCAGTGT GGAAAGAAGA GACCCCCTGG CGGCCCTGGC CCGGGAATAC
    GGCGGCTCCA AGCGCAACGC TCTCCTCAGG TGGTGTCAGA GGAAGACAGA GGGCTACGCG
    AACATTGATA TCACCAATTT CAGCAGCAGC TGGAGTGATG GCTTGGCCTT TTGTGCTCTC
    CTCCACACCT ACCTGCCCGC CCACATCCCA TACCAGGAGC TGAACAGCCA GGAGAAAAAA
    AGAAATCTCT TGTTGGCATT TGAAGCAGCT GAAAGTGTAG GCATCAAACC CAGCCTGGAG
    CTCAGTGAGT TGCTGCACAC AGACCGGCCT GACTGGCAGA GCGTGATGCA GTATGTGGCC
    CAGATCTACA AGTACTTCGA GACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10170
    Clone ID Related Accession (Same CDS sequence) XM_002695819.4 , XM_015468072.1 , XM_002695819.5
    Accession Version XM_002695819.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002695819.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GACCAGTGAA GGAAGAGCCC GAGCCCTCAG AGGCGGACGC TCCTGGCCGG
    TGGCCCAGCG CCTATGTGAA CAGGACTCCT CCAACACCTT CAGAGTCTGT GACCACTGTA
    AAGTCCCTCA TCAAGTCATT TGACTTGGGA CGACCAGGTG GAGCTGGGCA GAATATTTCT
    GTCCACAAGA CCCCCCGGAG TCCCCTGAGT GGGATCCCTG TGAGGACGCC CCCAGCTGCT
    GCTGTTTCTC CAATGCAGAG GCATTCTGCC TTCAGCAGTG CTCGGCCGGC CAGCAAAGGA
    GAGATTCAAC GCTTGGATCT CCCAGACCTT CCCCTCTCAG ATCTCCTGAA GGGAAGAGCT
    GAGGACCTGA AGCCAAACCC TTATCTCCGT AAGAGCCCCT CTCTCGAGTC CCTGAGCAGA
    CCCCCTTCTC TGGGCTTCGG GGGCTCCAGG CTGCTGGGCA CCTCCCCTGC GGGCTGGAAG
    CCGCAGAGCA AACTCAGTGT GGAAAGAAGA GACCCCCTGG CGGCCCTGGC CCGGGAATAC
    GGCGGCTCCA AGCGCAACGC TCTCCTCAGG TGGTGTCAGA GGAAGACAGA GGGCTACGCG
    AACATTGATA TCACCAATTT CAGCAGCAGC TGGAGTGATG GCTTGGCCTT TTGTGCTCTC
    CTCCACACCT ACCTGCCCGC CCACATCCCA TACCAGGAGC TGAACAGCCA GGAGAAAAAA
    AGAAATCTCT TGTTGGCATT TGAAGCAGCT GAAAGTGTAG GCATCAAACC CAGCCTGGAG
    CTCAGTGAGT TGCTGCACAC AGACCGGCCT GACTGGCAGA GCGTGATGCA GTATGTGGCC
    CAGATCTACA AGTACTTCGA GACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002695865.2
    CDS177..3383
    Translation

    Target ORF information:

    RefSeq Version XM_002695819.5
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002695819.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GACCAGTGAA GGAAGAGCCC GAGCCCTCAG AGGCGGACGC TCCTGGCCGG
    TGGCCCAGCG CCTATGTGAA CAGGACTCCT CCAACACCTT CAGAGTCTGT GACCACTGTA
    AAGTCCCTCA TCAAGTCATT TGACTTGGGA CGACCAGGTG GAGCTGGGCA GAATATTTCT
    GTCCACAAGA CCCCCCGGAG TCCCCTGAGT GGGATCCCTG TGAGGACGCC CCCAGCTGCT
    GCTGTTTCTC CAATGCAGAG GCATTCTGCC TTCAGCAGTG CTCGGCCGGC CAGCAAAGGA
    GAGATTCAAC GCTTGGATCT CCCAGACCTT CCCCTCTCAG ATCTCCTGAA GGGAAGAGCT
    GAGGACCTGA AGCCAAACCC TTATCTCCGT AAGAGCCCCT CTCTCGAGTC CCTGAGCAGA
    CCCCCTTCTC TGGGCTTCGG GGGCTCCAGG CTGCTGGGCA CCTCCCCTGC GGGCTGGAAG
    CCGCAGAGCA AACTCAGTGT GGAAAGAAGA GACCCCCTGG CGGCCCTGGC CCGGGAATAC
    GGCGGCTCCA AGCGCAACGC TCTCCTCAGG TGGTGTCAGA GGAAGACAGA GGGCTACGCG
    AACATTGATA TCACCAATTT CAGCAGCAGC TGGAGTGATG GCTTGGCCTT TTGTGCTCTC
    CTCCACACCT ACCTGCCCGC CCACATCCCA TACCAGGAGC TGAACAGCCA GGAGAAAAAA
    AGAAATCTCT TGTTGGCATT TGAAGCAGCT GAAAGTGTAG GCATCAAACC CAGCCTGGAG
    CTCAGTGAGT TGCTGCACAC AGACCGGCCT GACTGGCAGA GCGTGATGCA GTATGTGGCC
    CAGATCTACA AGTACTTCGA GACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10171
    Clone ID Related Accession (Same CDS sequence) XM_005220460.4 , XM_005220460.3 , XM_015468077.1
    Accession Version XM_005220460.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220460.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GACCAGTGAA GGAAGAGCCC GAGCCCTCAG AGGCGGACGC TCCTGGCCGG
    TGGCCCAGCG CCTATGTGAA CAGGACTCCT CCAACACCTT CAGAGTCTGT GACCACTGTA
    AAGTCCCTCA TCAAGTCATT TGACTTGGGA CGACCAGGTG GAGCTGGGCA GAATATTTCT
    GTCCACAAGA CCCCCCGGAG TCCCCTGAGT GGGATCCCTG TGAGGACGCC CCCAGCTGCT
    GCTGTTTCTC CAATGCAGAG GCATTCTGCC TTCAGCAGTG CTCGGCCGGC CAGCAAAGGA
    GAGATTCAAC GCTTGGATCT CCCAGACCTT CCCCTCTCAG ATCTCCTGAA GGGAAGAGCT
    GAGGACCTGA AGCCAAACCC TTATCTCCGT AAGAGCCCCT CTCTCGAGTC CCTGAGCAGA
    CCCCCTTCTC TGGGCTTCGG GGGCTCCAGG CTGCTGGGCA CCTCCCCTGC GGGCTGGAAG
    CCGCAGAGCA AACTCAGTGT GGAAAGAAGA GACCCCCTGG CGGCCCTGGC CCGGGAATAC
    GGCGGCTCCA AGCGCAACGC TCTCCTCAGG TGGTGTCAGA GGAAGACAGA GGGCTACGCG
    AAAAGAAATC TCTTGTTGGC ATTTGAAGCA GCTGAAAGTG TAGGCATCAA ACCCAGCCTG
    GAGCTCAGTG AGTTGCTGCA CACAGACCGG CCTGACTGGC AGAGCGTGAT GCAGTATGTG
    GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220517.1
    CDS139..3228
    Translation

    Target ORF information:

    RefSeq Version XM_005220460.4
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220460.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GACCAGTGAA GGAAGAGCCC GAGCCCTCAG AGGCGGACGC TCCTGGCCGG
    TGGCCCAGCG CCTATGTGAA CAGGACTCCT CCAACACCTT CAGAGTCTGT GACCACTGTA
    AAGTCCCTCA TCAAGTCATT TGACTTGGGA CGACCAGGTG GAGCTGGGCA GAATATTTCT
    GTCCACAAGA CCCCCCGGAG TCCCCTGAGT GGGATCCCTG TGAGGACGCC CCCAGCTGCT
    GCTGTTTCTC CAATGCAGAG GCATTCTGCC TTCAGCAGTG CTCGGCCGGC CAGCAAAGGA
    GAGATTCAAC GCTTGGATCT CCCAGACCTT CCCCTCTCAG ATCTCCTGAA GGGAAGAGCT
    GAGGACCTGA AGCCAAACCC TTATCTCCGT AAGAGCCCCT CTCTCGAGTC CCTGAGCAGA
    CCCCCTTCTC TGGGCTTCGG GGGCTCCAGG CTGCTGGGCA CCTCCCCTGC GGGCTGGAAG
    CCGCAGAGCA AACTCAGTGT GGAAAGAAGA GACCCCCTGG CGGCCCTGGC CCGGGAATAC
    GGCGGCTCCA AGCGCAACGC TCTCCTCAGG TGGTGTCAGA GGAAGACAGA GGGCTACGCG
    AAAAGAAATC TCTTGTTGGC ATTTGAAGCA GCTGAAAGTG TAGGCATCAA ACCCAGCCTG
    GAGCTCAGTG AGTTGCTGCA CACAGACCGG CCTGACTGGC AGAGCGTGAT GCAGTATGTG
    GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10171
    Clone ID Related Accession (Same CDS sequence) XM_005220460.4 , XM_005220460.3 , XM_015468077.1
    Accession Version XM_005220460.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220460.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GACCAGTGAA GGAAGAGCCC GAGCCCTCAG AGGCGGACGC TCCTGGCCGG
    TGGCCCAGCG CCTATGTGAA CAGGACTCCT CCAACACCTT CAGAGTCTGT GACCACTGTA
    AAGTCCCTCA TCAAGTCATT TGACTTGGGA CGACCAGGTG GAGCTGGGCA GAATATTTCT
    GTCCACAAGA CCCCCCGGAG TCCCCTGAGT GGGATCCCTG TGAGGACGCC CCCAGCTGCT
    GCTGTTTCTC CAATGCAGAG GCATTCTGCC TTCAGCAGTG CTCGGCCGGC CAGCAAAGGA
    GAGATTCAAC GCTTGGATCT CCCAGACCTT CCCCTCTCAG ATCTCCTGAA GGGAAGAGCT
    GAGGACCTGA AGCCAAACCC TTATCTCCGT AAGAGCCCCT CTCTCGAGTC CCTGAGCAGA
    CCCCCTTCTC TGGGCTTCGG GGGCTCCAGG CTGCTGGGCA CCTCCCCTGC GGGCTGGAAG
    CCGCAGAGCA AACTCAGTGT GGAAAGAAGA GACCCCCTGG CGGCCCTGGC CCGGGAATAC
    GGCGGCTCCA AGCGCAACGC TCTCCTCAGG TGGTGTCAGA GGAAGACAGA GGGCTACGCG
    AAAAGAAATC TCTTGTTGGC ATTTGAAGCA GCTGAAAGTG TAGGCATCAA ACCCAGCCTG
    GAGCTCAGTG AGTTGCTGCA CACAGACCGG CCTGACTGGC AGAGCGTGAT GCAGTATGTG
    GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220517.1
    CDS488..3577
    Translation

    Target ORF information:

    RefSeq Version XM_005220460.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220460.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GACCAGTGAA GGAAGAGCCC GAGCCCTCAG AGGCGGACGC TCCTGGCCGG
    TGGCCCAGCG CCTATGTGAA CAGGACTCCT CCAACACCTT CAGAGTCTGT GACCACTGTA
    AAGTCCCTCA TCAAGTCATT TGACTTGGGA CGACCAGGTG GAGCTGGGCA GAATATTTCT
    GTCCACAAGA CCCCCCGGAG TCCCCTGAGT GGGATCCCTG TGAGGACGCC CCCAGCTGCT
    GCTGTTTCTC CAATGCAGAG GCATTCTGCC TTCAGCAGTG CTCGGCCGGC CAGCAAAGGA
    GAGATTCAAC GCTTGGATCT CCCAGACCTT CCCCTCTCAG ATCTCCTGAA GGGAAGAGCT
    GAGGACCTGA AGCCAAACCC TTATCTCCGT AAGAGCCCCT CTCTCGAGTC CCTGAGCAGA
    CCCCCTTCTC TGGGCTTCGG GGGCTCCAGG CTGCTGGGCA CCTCCCCTGC GGGCTGGAAG
    CCGCAGAGCA AACTCAGTGT GGAAAGAAGA GACCCCCTGG CGGCCCTGGC CCGGGAATAC
    GGCGGCTCCA AGCGCAACGC TCTCCTCAGG TGGTGTCAGA GGAAGACAGA GGGCTACGCG
    AAAAGAAATC TCTTGTTGGC ATTTGAAGCA GCTGAAAGTG TAGGCATCAA ACCCAGCCTG
    GAGCTCAGTG AGTTGCTGCA CACAGACCGG CCTGACTGGC AGAGCGTGAT GCAGTATGTG
    GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10171
    Clone ID Related Accession (Same CDS sequence) XM_005220460.4 , XM_005220460.3 , XM_015468077.1
    Accession Version XM_015468077.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GACCAGTGAA GGAAGAGCCC GAGCCCTCAG AGGCGGACGC TCCTGGCCGG
    TGGCCCAGCG CCTATGTGAA CAGGACTCCT CCAACACCTT CAGAGTCTGT GACCACTGTA
    AAGTCCCTCA TCAAGTCATT TGACTTGGGA CGACCAGGTG GAGCTGGGCA GAATATTTCT
    GTCCACAAGA CCCCCCGGAG TCCCCTGAGT GGGATCCCTG TGAGGACGCC CCCAGCTGCT
    GCTGTTTCTC CAATGCAGAG GCATTCTGCC TTCAGCAGTG CTCGGCCGGC CAGCAAAGGA
    GAGATTCAAC GCTTGGATCT CCCAGACCTT CCCCTCTCAG ATCTCCTGAA GGGAAGAGCT
    GAGGACCTGA AGCCAAACCC TTATCTCCGT AAGAGCCCCT CTCTCGAGTC CCTGAGCAGA
    CCCCCTTCTC TGGGCTTCGG GGGCTCCAGG CTGCTGGGCA CCTCCCCTGC GGGCTGGAAG
    CCGCAGAGCA AACTCAGTGT GGAAAGAAGA GACCCCCTGG CGGCCCTGGC CCGGGAATAC
    GGCGGCTCCA AGCGCAACGC TCTCCTCAGG TGGTGTCAGA GGAAGACAGA GGGCTACGCG
    AAAAGAAATC TCTTGTTGGC ATTTGAAGCA GCTGAAAGTG TAGGCATCAA ACCCAGCCTG
    GAGCTCAGTG AGTTGCTGCA CACAGACCGG CCTGACTGGC AGAGCGTGAT GCAGTATGTG
    GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323563.1
    CDS488..3577
    Translation

    Target ORF information:

    RefSeq Version XM_015468077.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468077.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAAG GACTCCCTGG GACAAACATG AGGAGCGCAG CCAAGCCCTG GAGTCCAGTG 
    ACCAGGGCAG GGAGCCATGG GGTGGACCGC CCCCGGCCCC TCCCCGTCCC CTCTCCCGGC
    ATGAAGAGCT CCAAGTCTTC CACCTCGCTG GCTTTTGAGT CCCGACTCAG CAAGCTCAAG
    CGCGCCAGCA GCGAGGACAC CCTCAACAAG CCCGGGGCGG CCGTCGCTTC CGGGGCAGCA
    GCTCGGCTGA AAAAGACGTC CACGTCCGGG GCCATCTCCG AGCTCACGGA GAGCCGCTTG
    AGGGGCCCCT CAGGGTCCGT GACAGCTACC AAGAGGACAG GCATTCCTGC CCCTCGAGAA
    CTTGCCGTCA CTGTCTCAAG AGACAGAACT GTGCCACGCG GTCCCTCCAA CTCAAGGAAA
    GTGACGACCA GCCCCACCTC TTCCAGCACG CCCACCCCCA CCAAGCACGT GCGGGCTGCT
    CCCCCAAGAC CCCGGCCGGA GCATGAAGGT GCAGAGAAGG CTGTGCTCGA GTCCCAGGTC
    CGGGAGCTCT TGGCAGAAGC TAAAGAGAAA GACAGTGAGA TCAACAGGCT CCGAAGTGAA
    CTCAAGAAAT GGATGGAGAA ACGGACTCAG GACAGTGAAG GCGGGGATGC TTTGGACTCC
    AGTGTGGACG GAGCATCAGT CTTGCCAGGT GACTCGGAAC CTTTGATCCG AGCCCTTGAG
    GAGAAGAACA AGAGCTTCCA GAAAGAGCTC GCAGAGCTCG AGGAAGAAAA CCGGGCCTTG
    AAAGAGAAAC TGACTTATTT TGAGCATTCC CCAGATTCAG AGGGGGGAGC AAGTCACACC
    GGGGACAGCA GCTGCCCGAC GTCCGTAACT CAGGAGTCCA GCTTCGGGAG CCCGACGGGG
    AATGAGGCGT TGAGTGAAAT TGATGTGTAC AGAAAAAACC CACATGAGGG TGCCCTGCGG
    ACATCAGGCT CTTCCAGCAG TGATGTCACC AAGGCCTCCT TGTCCCCGGA TGCCTCTGAC
    TTCGAACACA TCCCAGCAGA CACCCTGGGG CCCCTGTCCC CTGCTGGGAG CCTTGTCAAG
    AGCTCCAAGT GCTCGGCAGC CGGCAGCTCC CCAAACACAG CGAGCGAGCT GTCCTTGGCG
    TCCCTGGCAG AGAAGATCCA CAGGATGGAG GAGAACCAGC ACAGCACGGC TGAGGAGCTG
    CAGGCCACGC TGCAGGAGCT GTCTGACCAG CAGCAGATGG TGCAGGAGCT GACGGCTGAA
    AATGAGAAGC TGGCGGATGA GAAGACGCTC CTGGAAACGT CCTTCCATCA GCACCGGGAG
    AGGGCGGAGC AGCTGAGCCA GGAGAATGAG AAGCTTATGA CCCTCCTGCA GGAGCGTGTC
    AAGGGCGAGG AGCCTGGCGC CCAGGAAGGC AAGATCCTGG AGCTGGAACA GAAGTGCACA
    GAGATCCTGG AGCAGGGCCG CTTCGAGCGC GAGAAGCTGC TGAGCATCCA ACAGCAGCTG
    ACGTGCCGCC TGCGCGCCGT CGAGGAGGAG CACCGCAGTG CTCTGGAGCT GGTCCGGCAC
    CTGCAGGAGG TGAATGGGGA GCTGACCACC CGCCTGGAGC TGGAGCAGCA CAGCGGCGGC
    CCCACAGCCG AGATCCCAGC AGCGCGTCAG CTTCCCGTGG AAGGGCTCCA GGTAGAGAAC
    GGGTCTTTGC AGGCCTCCCT GGAGAACGAG GAGCAGAAAA CTGGAGGGGC CAGCCCCCTG
    GGGCCCATGG CTGAGGGCTG CGACGTCCAG GAAATGCTGA CCGTGGCTCG AGCCGAGAAG
    GACCAGCTGG AACAGTCCTG CTCAGAGCTC AGGCAGGAGC TGCTGAAGGC ACATGGAGAG
    GTGAAGCGCG TTTCAAGCCT GCTGGCCAAG GTGGAAAAGG ATTACTCTTA CCTAAAGGAG
    ATCTGCGATC ACCAGGCAGA GCAGCTGAGC AGAACCAGCC TGAAGCTACA AGAGAAAGCA
    TCAGAGAGTG ACGCAGAGAT CAAAGACATG AAGGAGACCA TATTCGAGCT GGAGGACCAG
    GTGGAGCAGC ATCGCGCTGT CAAGTTGCAC AACAACCAGC TCATCAGTGA GCTGGAAAGC
    AGCGTGATGA AGCTGGAGGA ACAGAAGTCA GACCTGGAGA GGCAGCTGAA GACTCTGACG
    AAGCAGATAA AGGACGAGAC GGAAGAGTGG CGGCGCTTCC AGGCTGACCT GCAGACGGCG
    GTAGTGGTGG CCAATGACAT CAAGGTTGAG GCCCAGCAGG AGCTGCGCGC TGTCAAGCGG
    AGGCTGCTGG AGGAGGAAGA GAAGAACGCC CGGCTGCAGA AGGCACTGGG GGACGCACAG
    GCCCGCGGAA GACCAGTGAA GGAAGAGCCC GAGCCCTCAG AGGCGGACGC TCCTGGCCGG
    TGGCCCAGCG CCTATGTGAA CAGGACTCCT CCAACACCTT CAGAGTCTGT GACCACTGTA
    AAGTCCCTCA TCAAGTCATT TGACTTGGGA CGACCAGGTG GAGCTGGGCA GAATATTTCT
    GTCCACAAGA CCCCCCGGAG TCCCCTGAGT GGGATCCCTG TGAGGACGCC CCCAGCTGCT
    GCTGTTTCTC CAATGCAGAG GCATTCTGCC TTCAGCAGTG CTCGGCCGGC CAGCAAAGGA
    GAGATTCAAC GCTTGGATCT CCCAGACCTT CCCCTCTCAG ATCTCCTGAA GGGAAGAGCT
    GAGGACCTGA AGCCAAACCC TTATCTCCGT AAGAGCCCCT CTCTCGAGTC CCTGAGCAGA
    CCCCCTTCTC TGGGCTTCGG GGGCTCCAGG CTGCTGGGCA CCTCCCCTGC GGGCTGGAAG
    CCGCAGAGCA AACTCAGTGT GGAAAGAAGA GACCCCCTGG CGGCCCTGGC CCGGGAATAC
    GGCGGCTCCA AGCGCAACGC TCTCCTCAGG TGGTGTCAGA GGAAGACAGA GGGCTACGCG
    AAAAGAAATC TCTTGTTGGC ATTTGAAGCA GCTGAAAGTG TAGGCATCAA ACCCAGCCTG
    GAGCTCAGTG AGTTGCTGCA CACAGACCGG CCTGACTGGC AGAGCGTGAT GCAGTATGTG
    GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46582
    Clone ID Related Accession (Same CDS sequence) XM_010816097.2 , XM_010816096.2 , XM_015458758.2 , XM_015468076.1 , XM_015458758.1 , XM_015468073.1 , XM_010816097.3 , XM_015468074.1 , XM_015468075.1 , XM_015458759.1
    Accession Version XM_010816097.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816097.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814399.1
    CDS416..3595
    Translation

    Target ORF information:

    RefSeq Version XM_010816097.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816097.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46582
    Clone ID Related Accession (Same CDS sequence) XM_010816097.2 , XM_010816096.2 , XM_015458758.2 , XM_015468076.1 , XM_015458758.1 , XM_015468073.1 , XM_010816097.3 , XM_015468074.1 , XM_015468075.1 , XM_015458759.1
    Accession Version XM_010816096.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814398.1
    CDS177..3356
    Translation

    Target ORF information:

    RefSeq Version XM_010816096.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816096.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46582
    Clone ID Related Accession (Same CDS sequence) XM_010816097.2 , XM_010816096.2 , XM_015458758.2 , XM_015468076.1 , XM_015458758.1 , XM_015468073.1 , XM_010816097.3 , XM_015468074.1 , XM_015468075.1 , XM_015458759.1
    Accession Version XM_015458758.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314244.1
    CDS555..3734
    Translation

    Target ORF information:

    RefSeq Version XM_015458758.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458758.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46582
    Clone ID Related Accession (Same CDS sequence) XM_010816097.2 , XM_010816096.2 , XM_015458758.2 , XM_015468076.1 , XM_015458758.1 , XM_015468073.1 , XM_010816097.3 , XM_015468074.1 , XM_015468075.1 , XM_015458759.1
    Accession Version XM_015468076.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323562.1
    CDS69..3248
    Translation

    Target ORF information:

    RefSeq Version XM_015468076.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468076.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46582
    Clone ID Related Accession (Same CDS sequence) XM_010816097.2 , XM_010816096.2 , XM_015458758.2 , XM_015468076.1 , XM_015458758.1 , XM_015468073.1 , XM_010816097.3 , XM_015468074.1 , XM_015468075.1 , XM_015458759.1
    Accession Version XM_015458758.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314244.1
    CDS522..3701
    Translation

    Target ORF information:

    RefSeq Version XM_015458758.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458758.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46582
    Clone ID Related Accession (Same CDS sequence) XM_010816097.2 , XM_010816096.2 , XM_015458758.2 , XM_015468076.1 , XM_015458758.1 , XM_015468073.1 , XM_010816097.3 , XM_015468074.1 , XM_015468075.1 , XM_015458759.1
    Accession Version XM_015468073.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323559.1
    CDS416..3595
    Translation

    Target ORF information:

    RefSeq Version XM_015468073.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468073.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46582
    Clone ID Related Accession (Same CDS sequence) XM_010816097.2 , XM_010816096.2 , XM_015458758.2 , XM_015468076.1 , XM_015458758.1 , XM_015468073.1 , XM_010816097.3 , XM_015468074.1 , XM_015468075.1 , XM_015458759.1
    Accession Version XM_010816097.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816097.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814399.1
    CDS448..3627
    Translation

    Target ORF information:

    RefSeq Version XM_010816097.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816097.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46582
    Clone ID Related Accession (Same CDS sequence) XM_010816097.2 , XM_010816096.2 , XM_015458758.2 , XM_015468076.1 , XM_015458758.1 , XM_015468073.1 , XM_010816097.3 , XM_015468074.1 , XM_015468075.1 , XM_015458759.1
    Accession Version XM_015468074.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323560.1
    CDS177..3356
    Translation

    Target ORF information:

    RefSeq Version XM_015468074.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468074.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46582
    Clone ID Related Accession (Same CDS sequence) XM_010816097.2 , XM_010816096.2 , XM_015458758.2 , XM_015468076.1 , XM_015458758.1 , XM_015468073.1 , XM_010816097.3 , XM_015468074.1 , XM_015468075.1 , XM_015458759.1
    Accession Version XM_015468075.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323561.1
    CDS522..3701
    Translation

    Target ORF information:

    RefSeq Version XM_015468075.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468075.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46582
    Clone ID Related Accession (Same CDS sequence) XM_010816097.2 , XM_010816096.2 , XM_015458758.2 , XM_015468076.1 , XM_015458758.1 , XM_015468073.1 , XM_010816097.3 , XM_015468074.1 , XM_015468075.1 , XM_015458759.1
    Accession Version XM_015458759.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314245.1
    CDS69..3248
    Translation

    Target ORF information:

    RefSeq Version XM_015458759.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 (SPECC1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458759.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCG CAGCCAAGCC CTGGAGTCCA GTGACCAGGG CAGGGAGCCA TGGGGTGGAC 
    CGCCCCCGGC CCCTCCCCGT CCCCTCTCCC GGCATGAAGA GCTCCAAGTC TTCCACCTCG
    CTGGCTTTTG AGTCCCGACT CAGCAAGCTC AAGCGCGCCA GCAGCGAGGA CACCCTCAAC
    AAGCCCGGGG CGGCCGTCGC TTCCGGGGCA GCAGCTCGGC TGAAAAAGAC GTCCACGTCC
    GGGGCCATCT CCGAGCTCAC GGAGAGCCGC TTGAGGGGCC CCTCAGGGTC CGTGACAGCT
    ACCAAGAGGA CAGGCATTCC TGCCCCTCGA GAACTTGCCG TCACTGTCTC AAGAGACAGA
    ACTGTGCCAC GCGGTCCCTC CAACTCAAGG AAAGTGACGA CCAGCCCCAC CTCTTCCAGC
    ACGCCCACCC CCACCAAGCA CGTGCGGGCT GCTCCCCCAA GACCCCGGCC GGAGCATGAA
    GGTGCAGAGA AGGCTGTGCT CGAGTCCCAG GTCCGGGAGC TCTTGGCAGA AGCTAAAGAG
    AAAGACAGTG AGATCAACAG GCTCCGAAGT GAACTCAAGA AATGGATGGA GAAACGGACT
    CAGGACAGTG AAGGCGGGGA TGCTTTGGAC TCCAGTGTGG ACGGAGCATC AGTCTTGCCA
    GGTGACTCGG AACCTTTGAT CCGAGCCCTT GAGGAGAAGA ACAAGAGCTT CCAGAAAGAG
    CTCGCAGAGC TCGAGGAAGA AAACCGGGCC TTGAAAGAGA AACTGACTTA TTTTGAGCAT
    TCCCCAGATT CAGAGGGGGG AGCAAGTCAC ACCGGGGACA GCAGCTGCCC GACGTCCGTA
    ACTCAGGAGT CCAGCTTCGG GAGCCCGACG GGGAATGAGG CGTTGAGTGA AATTGATGTG
    TACAGAAAAA ACCCACATGA GGGTGCCCTG CGGACATCAG GCTCTTCCAG CAGTGATGTC
    ACCAAGGCCT CCTTGTCCCC GGATGCCTCT GACTTCGAAC ACATCCCAGC AGACACCCTG
    GGGCCCCTGT CCCCTGCTGG GAGCCTTGTC AAGAGCTCCA AGTGCTCGGC AGCCGGCAGC
    TCCCCAAACA CAGCGAGCGA GCTGTCCTTG GCGTCCCTGG CAGAGAAGAT CCACAGGATG
    GAGGAGAACC AGCACAGCAC GGCTGAGGAG CTGCAGGCCA CGCTGCAGGA GCTGTCTGAC
    CAGCAGCAGA TGGTGCAGGA GCTGACGGCT GAAAATGAGA AGCTGGCGGA TGAGAAGACG
    CTCCTGGAAA CGTCCTTCCA TCAGCACCGG GAGAGGGCGG AGCAGCTGAG CCAGGAGAAT
    GAGAAGCTTA TGACCCTCCT GCAGGAGCGT GTCAAGGGCG AGGAGCCTGG CGCCCAGGAA
    GGCAAGATCC TGGAGCTGGA ACAGAAGTGC ACAGAGATCC TGGAGCAGGG CCGCTTCGAG
    CGCGAGAAGC TGCTGAGCAT CCAACAGCAG CTGACGTGCC GCCTGCGCGC CGTCGAGGAG
    GAGCACCGCA GTGCTCTGGA GCTGGTCCGG CACCTGCAGG AGGTGAATGG GGAGCTGACC
    ACCCGCCTGG AGCTGGAGCA GCACAGCGGC GGCCCCACAG CCGAGATCCC AGCAGCGCGT
    CAGCTTCCCG TGGAAGGGCT CCAGGTAGAG AACGGGTCTT TGCAGGCCTC CCTGGAGAAC
    GAGGAGCAGA AAACTGGAGG GGCCAGCCCC CTGGGGCCCA TGGCTGAGGG CTGCGACGTC
    CAGGAAATGC TGACCGTGGC TCGAGCCGAG AAGGACCAGC TGGAACAGTC CTGCTCAGAG
    CTCAGGCAGG AGCTGCTGAA GGCACATGGA GAGGTGAAGC GCGTTTCAAG CCTGCTGGCC
    AAGGTGGAAA AGGATTACTC TTACCTAAAG GAGATCTGCG ATCACCAGGC AGAGCAGCTG
    AGCAGAACCA GCCTGAAGCT ACAAGAGAAA GCATCAGAGA GTGACGCAGA GATCAAAGAC
    ATGAAGGAGA CCATATTCGA GCTGGAGGAC CAGGTGGAGC AGCATCGCGC TGTCAAGTTG
    CACAACAACC AGCTCATCAG TGAGCTGGAA AGCAGCGTGA TGAAGCTGGA GGAACAGAAG
    TCAGACCTGG AGAGGCAGCT GAAGACTCTG ACGAAGCAGA TAAAGGACGA GACGGAAGAG
    TGGCGGCGCT TCCAGGCTGA CCTGCAGACG GCGGTAGTGG TGGCCAATGA CATCAAGGTT
    GAGGCCCAGC AGGAGCTGCG CGCTGTCAAG CGGAGGCTGC TGGAGGAGGA AGAGAAGAAC
    GCCCGGCTGC AGAAGGCACT GGGGGACGCA CAGGCCCGCG GAAGACCAGT GAAGGAAGAG
    CCCGAGCCCT CAGAGGCGGA CGCTCCTGGC CGGTGGCCCA GCGCCTATGT GAACAGGACT
    CCTCCAACAC CTTCAGAGTC TGTGACCACT GTAAAGTCCC TCATCAAGTC ATTTGACTTG
    GGACGACCAG GTGGAGCTGG GCAGAATATT TCTGTCCACA AGACCCCCCG GAGTCCCCTG
    AGTGGGATCC CTGTGAGGAC GCCCCCAGCT GCTGCTGTTT CTCCAATGCA GAGGCATTCT
    GCCTTCAGCA GTGCTCGGCC GGCCAGCAAA GGAGAGATTC AACGCTTGGA TCTCCCAGAC
    CTTCCCCTCT CAGATCTCCT GAAGGGAAGA GCTGAGGACC TGAAGCCAAA CCCTTATCTC
    CGTAAGAGCC CCTCTCTCGA GTCCCTGAGC AGACCCCCTT CTCTGGGCTT CGGGGGCTCC
    AGGCTGCTGG GCACCTCCCC TGCGGGCTGG AAGCCGCAGA GCAAACTCAG TGTGGAAAGA
    AGAGACCCCC TGGCGGCCCT GGCCCGGGAA TACGGCGGCT CCAAGCGCAA CGCTCTCCTC
    AGGTGGTGTC AGAGGAAGAC AGAGGGCTAC GCGAACATTG ATATCACCAA TTTCAGCAGC
    AGCTGGAGTG ATGGCTTGGC CTTTTGTGCT CTCCTCCACA CCTACCTGCC CGCCCACATC
    CCATACCAGG AGCTGAACAG CCAGGAGAAA AAAAGAAATC TCTTGTTGGC ATTTGAAGCA
    GCTGAAAGTG TAGGCATCAA ACCCAGCCTG GAGCTCAGTG AGTTGCTGCA CACAGACCGG
    CCTGACTGGC AGAGCGTGAT GCAGTATGTG GCCCAGATCT ACAAGTACTT CGAGACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.