MEGF6B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	MEGF6B
Entrez Gene ID	557764
Full Name	multiple EGF-like-domains 6b
General protein information	Preferred Names multiple EGF-like-domains 6b Names multiple epidermal growth factor-like domains protein 6
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306294.3	XP_009304569.1	multiple epidermal growth factor-like domains protein 6 isoform X1
XM_021479906.1	XP_021335581.1	multiple epidermal growth factor-like domains protein 6 isoform X2
XM_021479907.1	XP_021335582.1	multiple epidermal growth factor-like domains protein 6 isoform X3
XM_009306294.2	XP_009304569.1	multiple epidermal growth factor-like domains protein 6 isoform X1
XM_009306297.2	XP_009304572.1	multiple epidermal growth factor-like domains protein 6 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MEGF6	XP_546739.4
Mus musculus (house mouse)	Megf6	NP_001156449.1
Rattus norvegicus (Norway rat)	Megf6	NP_075244.1
Rattus norvegicus (Norway rat)	LOC100911486	XP_006239572.1
Danio rerio (zebrafish)	LOC557764	XP_685976.6
Caenorhabditis elegans (roundworm)	CELE_Y64G10A.7	NP_001255803.1
Pan troglodytes (chimpanzee)	MEGF6	XP_513736.3
Gallus gallus (chicken)	MEGF6	XP_004947431.1
Homo sapiens (human)	MEGF6	NP_001400.3
Bos taurus (cattle)	MEGF6	XP_005217247.1
Danio rerio (zebrafish)	megf6a	XP_693497.6
Xenopus tropicalis (tropical clawed frog)	megf6	XP_002933906.2

Gene Ontology

The following MEGF6B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MEGF6B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa42665	XM_021479906.1 Latest version!	Danio rerio multiple EGF-like-domains 6b (megf6b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa42666	XM_021479907.1 Latest version!	Danio rerio multiple EGF-like-domains 6b (megf6b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa42664	XM_009306294.3 Latest version!	Danio rerio multiple EGF-like-domains 6b (megf6b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa42667	XM_009306297.2 Latest version!	Danio rerio multiple EGF-like-domains 6b (megf6b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa42664	XM_009306294.2	Danio rerio multiple EGF-like-domains 6b (megf6b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MEGF6B ( XM_021479906.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021335581.1 Danio rerio multiple EGF-like-domains 6b (megf6b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42665
Clone ID Related Accession (Same CDS sequence)	XM_021479906.1
Accession Version	XM_021479906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	multiple epidermal growth factor-like domains protein 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACG TGGATTTATT ACTTTCCTTA ATCATTCTAG TTGATGGAAT TAATGCAGTA 
TCGCGTCTCG GGAAGGACCT GAAGCCGCAC ATGCCGAATG TGTGTGCAGA GAAGGAGGTG 
AAGCTGGTGG CTCAGATGCA GCCGTGTGTT CAAGCCTTCA CACGAATGGT TAAAGCATGG 
AAACAAGGTT GTCAGGGTCA GTCCTGGTGT GTGGGATACG AGAGGAGAAC AGCTTATTAT 
ACTGCATACA GACAGGTGTA TCGACAGGAA CAGCAGACGG TTTACAAGTG TTGTCCAGGA 
TGGTCACAGC TCAACGGAGA GGCCGGATGC CTGTACCCGG TCTGCAGGTC TGGTGTGTGT 
TTTAATGGCG GTCAGTGTAA AGAAGGTTCC GCTCAGCTCT GTGTGTGTCC AGCAGGATTC 
AGCGGCCCCA GCTGCCAATA TGATGTGAAC GAGTGTGAAG AGACCAATGG AGGCTGTGAG 
GCTTTATGCT GTAACACCAT CGGCAGCTTC TACTGCAAGT GTCCCGCAGG TCAAGAGCTC 
AAGGAGGATG GCAAGACCTG CCAAGATATT GACGAGTGCC AGGTTCATAA CGGCGGCTGC 
CAGCACACAT GTGTGAACAC TAGGGGTTCG TATCACTGCC AGTGTAACGC CGGCTCTCGA 
CTGCATGTGG ACGGACGCAC CTGCATCGCT GTTCAGTCCT GCGGCGTTGG GAATGGCGGC 
TGTGAGCATT TCTGTGTCCA GCAGACGGCG GGTCATGTCC AGTGTCGCTG CAGACCTGAT 
TACAGACTGG ACGCGGATGG AAAACACTGC ACATTGGTGA ACCCGTGTGC GGAGCAGAAT 
GGCGGATGTT CTCAGATGTG TCATAGGGAT GGAGCTCAGG CCCGCTGTGA ATGTCATCCA 
GGTTATCAGC TCGCTGAAGA CGGCAAGACA TGTGAAGACA TTGATGAGTG TGCGTTGGGT 
CAGACGGACT GTGCTCATGG CTGTCGTAAC ACCAGAGGAT CCTTCATGTG TGTGTGTTCT 
GCTGCCTATG AGCTCGGGGT GGACGGTAAA CAGTGCTATC GTATTGAGAT GGAGATCGTG 
AACAGCTGTG ATCATGATAA TGGTGGTTGT TCTCATCACT GTGAACATTC GACCGCTGGT 
CCGCTGTGCA GCTGTAATGA AGGATATCAG CTGGACCACG ACCACAAGAC CTGCGTCGAT 
ATAAACGAGT GTGTCGACGG CAGCTCGTGT TGTGAACATG ACTGTACGAA CTATCCTGGA 
GGATACGAGT GTTACTGTAC AGCCGGATAT CGACTGAACA CTGACGGATG CAGCTGTGAT 
GATATAGATG AGTGTTTGGC TGTTAATGGA GGATGTGATC ATGCTTGTCT GAACAGCGCC 
GGCTCTTTCC AGTGTTCCTG TAGACGTGGA TATCGACTCG ATGAGGACAG AAGATCCTGC 
ATACTGATGG AGGAGTCAGT GGAGGCGTTG AGCAGCGGTC AGACAGTCAA TAAACCTGGA 
CCAGGACCTC AGCTCACCAT CCTGCAGGAC TTCGATCAGA TCCTGGAGAA ATACGAGGAC 
TACGAGGACG ACACTGGAGA ACTGCGTGCC GAGAGCACTT TAGCTGAGAA ATTCATTTGT 
TTGAACGATT CGTTCGGCGT TGACTGCAGT CTGTCCTGTG AGGACTGTGT GAACGGAGGA 
GTGTGTAACA AACACAGAAA TGCCTGTGAC TGTCCTGACG GATGGACTGG AGTCGTCTGT 
AATGAGACAT GCCCTGAGGG TTGGTTTGGC AGGAATTGCT CACTGTTGTG TAAGTGTAAG 
AACGGTGGTG TGTGTGATCC GGTGTCCGGG AGCTGTCGCT GTCCACCAGG GGTCAGTGGA 
GAGCTGTGTC AGGACGGCTG TCCGAAGGGT CTCTATGGGA AGTTTTGCAA TAAAAGGTGT 
AACTGCGCTA ATAACGGACG TTGTCATCGC ACTTATGGAG CGTGTTTGTG TGACCCGGGA 
CTGTATGGAC GATTCTGCCA CCTTCCGTGT CCGAAGTGGA TGTTTGGTCC CGGTTGTTCA 
GAAGAGTGTC AGTGTGTCCA GCAGAACACT ATAGAGTGTC ACCGTCATCA TGGAGACTGC 
ATCTGCAAAC CTGGATACAG AGGCCACACC TGCAAAGACG AGTGTGAATC AGGGTTTTTT 
GGCTCTGCTT GTGAGAATAA ATGCTCTTGT CCGCCTGGAG TGACCTGTGA TCATGTGACT 
GGAGCTTGTC AACCCAAATG TCCAGCTGGA AAAACAGGCG ACAAATGTGA CCAAGATTGT 
GTGGAAGGGA GATTTGGGAT TGGATGCTCT CAGTCTTGTG ACTGCTCTGG CGCACCATGT 
GACAAAGTGA CTGGTCAATG CACGTGTCCT GCTGGAACGT TTGGAAAGCG CTGTGAAAAA 
GAGTGTCCTG AGGGCCGCTG GGGTCTGGGC TGTCAGGAAT GGTGTCCGGT GTGCGAGAAC 
GGAGGCCGCT GTGACCGGCG TAACGGCACA TGTGTGTGTT CTCCTGGATT CATCGGCAGA 
TTGTGTCAGA ACTCATGTCC TGCGGGTCGC TTCGGTGTGG GCTGTCAGCT CAGATGTGTG 
TGTGATCTCG GAGTTCGCTG TAATCCTGTA AGCGGACGCT GCTCGTGTCC TCCAGGCTGG 
ACGGGACACA ACTGCAGGAA GGTGTGTGAC GCGGGCCGCT GGGGTCTGGA CTGTGAGAGT 
GTATGTGACT GTCGTAATGC TGACGGTGTG TGTGATGCTC AGACTGGACG CTGTCATTGT 
GAGGCCGGAT TTACTGGAGC ACGATGTGAC CAGAAATGCC CGGCTGGCTC GTTTGGGCCC 
GGCTGTAAGC ATCGCTGCCA GTGTGAAAAT CAGGCATCAT GTGACCACGT GGGCGGAGCT 
TGTACCTGTA AGACCGGCTG GACCGGAACC TTCTGTGAAA AACCCTGTCC GCAGGGTTTC 
TATGGTCTGG ACTGTCAGCA GAAGTGTGTG TGTTTGAACG GCGGTCTCTG TGATCATGTG 
AGCGGTCAGT GTTTCTGTAA GGCCGGATGG ATCGGGACGC GCTGTAACCA GACGTGTCCT 
GCGGGATGGT TTGGCGTGAG CTGTTCTCAG ACCTGTGTTT GTCACAATAA CAGCAGCTGT 
GATGCAGTGA GCGGTCAGTG TGAATGCGGT GCAGGCTGGA CAGGAGACAC ATGCTCACAG 
CGCTGTCCTG CAGGTGTGTT CGGGGACGGT TGTGTTCACA GGTGTTTGTG TCAGAACGGG 
GCCTCGTGTG ACCCTGAGAG TGGACACTGC TTCTGTCCTA GCGGCTGGAC GGGGGCCGCC 
TGTCAGCTTG AATGTCCAGC GGGGAGGTTC GGCTCAGACT GTCAGGACAG ATGTGAATGT 
GTGAATGGAG CTCAGTGTGA TGGTCAGACG GGTCGCTGTG TTTGTCCGCC GGGCTGGACA 
GGAGAGCGCT GTGAGAATGC ATGTCTGCCG GGCTCATTTG GTCAGGATTG TGTCCAGCGC 
TGCTCTTGTC CGTCCGGCTC CTCGTGTCAT CATGTGACCG GTCACTGTGG TTGTGCTCCA 
GGATTCACCG GCGACGGCTG TGAACACAGT TGTCTGCCTG GCACGTTCGG CCAGGACTGT 
AATCAGGTGT GTCAGTGTTC AGAAAGAAAC CAGCTCTGCC ACCCGGTGAC TGGAGTGTGT 
TACTGCGCGC CCGGATTCAC TGGACTCAAG TGTGAACAGA CGTGTCCGCA GGGTTTGTAC 
GGCTCAGGCT GTGAGCATCA GTGTGAGTGT CTGAATGGTG GAGTTTGTCT CTCAGACTCG 
GGATCCTGTC AGTGTCCAGC CGGATTCATT GGAGCTCAAT GCAATCAAAC ATGTCCAGCG 
GGTCGCTATG GTCTGGACTG TGCTCATGTG TCAGCGTGTG GGACAGGAGT GAGGAATGAT 
CCGGCTACAG GAAGATGCAC TGCACATCAA GTCTGTCCTC CAGGTTGGTT TGGTTCTGGC 
TGTTCTCAGC GTTGTGACTG CAGTAACAGA GGTGTGTGTG ACGGAGTCTC TGGAAACTGC 
TCATGTGCTC TCGGCTGGAC CGGCAAACAC TGTGATAAAG AGTGTCCTGA TGGCAGCTTT 
GGGCCTGACT GCTCTCTTCA GTGTTCCTGT CAGAATAACA GCACATGTGA CCGAGTGACA 
GGAAGTTGTC ATTGTCGTCC AGGATATTAC GGTCATCTGT GTGAACACGC GTGTCCGGCT 
GGTCTCTTCG GCTCTGGGTG TCAGCAGCAG TGTGACTGTA TGAACGGAGC CTCCTGTCAT 
CCGGATACAG GACAGTGTGT GTGTCCCGCT GGAGTCCACG GCCCGCGCTG TCAGCACGTG 
TGTGCTCAGG GCAGGTTTGG TGTGAACTGC TCTCAGTCAT GTGACTGTGC TGATGGAGCG 
TCATGTGACC CGGTCAGCGG CCGCTGTGTT TGTCCATCAG GGAAGACTGG AGTGCGGTGT 
GACAGCGACT GCAGTGTGAA CCGGTTTGGC CCTGACTGCT CTCTGCTGTG CGAGTGTGAA 
AATGGAGCTC AGTGCGACAG CAGAAATGGA CGCTGCTTCT GTCTGAGCGG CTGGATCGGC 
TTCACATGTT CACAGAGCGC TCCTCCAGAA CTGAACTCCA GACGGTCAGA AGAGGATCAG 
CTTCAGCTCA ACACTTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335581.1
CDS	680..5320
Translation	MKNVDLLLSLIILVDGINAVSRLGKDLKPHMPNVCAEKEVKLVAQMQPCVQAFTRMVKAWKQGCQGQSWCVGYERRTAYYTAYRQVYRQEQQTVYKCCPGWSQLNGEAGCLYPVCRSGVCFNGGQCKEGSAQLCVCPAGFSGPSCQYDVNECEETNGGCEALCCNTIGSFYCKCPAGQELKEDGKTCQDIDECQVHNGGCQHTCVNTRGSYHCQCNAGSRLHVDGRTCIAVQSCGVGNGGCEHFCVQQTAGHVQCRCRPDYRLDADGKHCTLVNPCAEQNGGCSQMCHRDGAQARCECHPGYQLAEDGKTCEDIDECALGQTDCAHGCRNTRGSFMCVCSAAYELGVDGKQCYRIEMEIVNSCDHDNGGCSHHCEHSTAGPLCSCNEGYQLDHDHKTCVDINECVDGSSCCEHDCTNYPGGYECYCTAGYRLNTDGCSCDDIDECLAVNGGCDHACLNSAGSFQCSCRRGYRLDEDRRSCILMEESVEALSSGQTVNKPGPGPQLTILQDFDQILEKYEDYEDDTGELRAESTLAEKFICLNDSFGVDCSLSCEDCVNGGVCNKHRNACDCPDGWTGVVCNETCPEGWFGRNCSLLCKCKNGGVCDPVSGSCRCPPGVSGELCQDGCPKGLYGKFCNKRCNCANNGRCHRTYGACLCDPGLYGRFCHLPCPKWMFGPGCSEECQCVQQNTIECHRHHGDCICKPGYRGHTCKDECESGFFGSACENKCSCPPGVTCDHVTGACQPKCPAGKTGDKCDQDCVEGRFGIGCSQSCDCSGAPCDKVTGQCTCPAGTFGKRCEKECPEGRWGLGCQEWCPVCENGGRCDRRNGTCVCSPGFIGRLCQNSCPAGRFGVGCQLRCVCDLGVRCNPVSGRCSCPPGWTGHNCRKVCDAGRWGLDCESVCDCRNADGVCDAQTGRCHCEAGFTGARCDQKCPAGSFGPGCKHRCQCENQASCDHVGGACTCKTGWTGTFCEKPCPQGFYGLDCQQKCVCLNGGLCDHVSGQCFCKAGWIGTRCNQTCPAGWFGVSCSQTCVCHNNSSCDAVSGQCECGAGWTGDTCSQRCPAGVFGDGCVHRCLCQNGASCDPESGHCFCPSGWTGAACQLECPAGRFGSDCQDRCECVNGAQCDGQTGRCVCPPGWTGERCENACLPGSFGQDCVQRCSCPSGSSCHHVTGHCGCAPGFTGDGCEHSCLPGTFGQDCNQVCQCSERNQLCHPVTGVCYCAPGFTGLKCEQTCPQGLYGSGCEHQCECLNGGVCLSDSGSCQCPAGFIGAQCNQTCPAGRYGLDCAHVSACGTGVRNDPATGRCTAHQVCPPGWFGSGCSQRCDCSNRGVCDGVSGNCSCALGWTGKHCDKECPDGSFGPDCSLQCSCQNNSTCDRVTGSCHCRPGYYGHLCEHACPAGLFGSGCQQQCDCMNGASCHPDTGQCVCPAGVHGPRCQHVCAQGRFGVNCSQSCDCADGASCDPVSGRCVCPSGKTGVRCDSDCSVNRFGPDCSLLCECENGAQCDSRNGRCFCLSGWIGFTCSQSAPPELNSRRSEEDQLQLNTS

Target ORF information:

RefSeq Version	XM_021479906.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio multiple EGF-like-domains 6b (megf6b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479906.1

ORF Insert Sequence:

ATGAAGAACG TGGATTTATT ACTTTCCTTA ATCATTCTAG TTGATGGAAT TAATGCAGTA 
TCGCGTCTCG GGAAGGACCT GAAGCCGCAC ATGCCGAATG TGTGTGCAGA GAAGGAGGTG 
AAGCTGGTGG CTCAGATGCA GCCGTGTGTT CAAGCCTTCA CACGAATGGT TAAAGCATGG 
AAACAAGGTT GTCAGGGTCA GTCCTGGTGT GTGGGATACG AGAGGAGAAC AGCTTATTAT 
ACTGCATACA GACAGGTGTA TCGACAGGAA CAGCAGACGG TTTACAAGTG TTGTCCAGGA 
TGGTCACAGC TCAACGGAGA GGCCGGATGC CTGTACCCGG TCTGCAGGTC TGGTGTGTGT 
TTTAATGGCG GTCAGTGTAA AGAAGGTTCC GCTCAGCTCT GTGTGTGTCC AGCAGGATTC 
AGCGGCCCCA GCTGCCAATA TGATGTGAAC GAGTGTGAAG AGACCAATGG AGGCTGTGAG 
GCTTTATGCT GTAACACCAT CGGCAGCTTC TACTGCAAGT GTCCCGCAGG TCAAGAGCTC 
AAGGAGGATG GCAAGACCTG CCAAGATATT GACGAGTGCC AGGTTCATAA CGGCGGCTGC 
CAGCACACAT GTGTGAACAC TAGGGGTTCG TATCACTGCC AGTGTAACGC CGGCTCTCGA 
CTGCATGTGG ACGGACGCAC CTGCATCGCT GTTCAGTCCT GCGGCGTTGG GAATGGCGGC 
TGTGAGCATT TCTGTGTCCA GCAGACGGCG GGTCATGTCC AGTGTCGCTG CAGACCTGAT 
TACAGACTGG ACGCGGATGG AAAACACTGC ACATTGGTGA ACCCGTGTGC GGAGCAGAAT 
GGCGGATGTT CTCAGATGTG TCATAGGGAT GGAGCTCAGG CCCGCTGTGA ATGTCATCCA 
GGTTATCAGC TCGCTGAAGA CGGCAAGACA TGTGAAGACA TTGATGAGTG TGCGTTGGGT 
CAGACGGACT GTGCTCATGG CTGTCGTAAC ACCAGAGGAT CCTTCATGTG TGTGTGTTCT 
GCTGCCTATG AGCTCGGGGT GGACGGTAAA CAGTGCTATC GTATTGAGAT GGAGATCGTG 
AACAGCTGTG ATCATGATAA TGGTGGTTGT TCTCATCACT GTGAACATTC GACCGCTGGT 
CCGCTGTGCA GCTGTAATGA AGGATATCAG CTGGACCACG ACCACAAGAC CTGCGTCGAT 
ATAAACGAGT GTGTCGACGG CAGCTCGTGT TGTGAACATG ACTGTACGAA CTATCCTGGA 
GGATACGAGT GTTACTGTAC AGCCGGATAT CGACTGAACA CTGACGGATG CAGCTGTGAT 
GATATAGATG AGTGTTTGGC TGTTAATGGA GGATGTGATC ATGCTTGTCT GAACAGCGCC 
GGCTCTTTCC AGTGTTCCTG TAGACGTGGA TATCGACTCG ATGAGGACAG AAGATCCTGC 
ATACTGATGG AGGAGTCAGT GGAGGCGTTG AGCAGCGGTC AGACAGTCAA TAAACCTGGA 
CCAGGACCTC AGCTCACCAT CCTGCAGGAC TTCGATCAGA TCCTGGAGAA ATACGAGGAC 
TACGAGGACG ACACTGGAGA ACTGCGTGCC GAGAGCACTT TAGCTGAGAA ATTCATTTGT 
TTGAACGATT CGTTCGGCGT TGACTGCAGT CTGTCCTGTG AGGACTGTGT GAACGGAGGA 
GTGTGTAACA AACACAGAAA TGCCTGTGAC TGTCCTGACG GATGGACTGG AGTCGTCTGT 
AATGAGACAT GCCCTGAGGG TTGGTTTGGC AGGAATTGCT CACTGTTGTG TAAGTGTAAG 
AACGGTGGTG TGTGTGATCC GGTGTCCGGG AGCTGTCGCT GTCCACCAGG GGTCAGTGGA 
GAGCTGTGTC AGGACGGCTG TCCGAAGGGT CTCTATGGGA AGTTTTGCAA TAAAAGGTGT 
AACTGCGCTA ATAACGGACG TTGTCATCGC ACTTATGGAG CGTGTTTGTG TGACCCGGGA 
CTGTATGGAC GATTCTGCCA CCTTCCGTGT CCGAAGTGGA TGTTTGGTCC CGGTTGTTCA 
GAAGAGTGTC AGTGTGTCCA GCAGAACACT ATAGAGTGTC ACCGTCATCA TGGAGACTGC 
ATCTGCAAAC CTGGATACAG AGGCCACACC TGCAAAGACG AGTGTGAATC AGGGTTTTTT 
GGCTCTGCTT GTGAGAATAA ATGCTCTTGT CCGCCTGGAG TGACCTGTGA TCATGTGACT 
GGAGCTTGTC AACCCAAATG TCCAGCTGGA AAAACAGGCG ACAAATGTGA CCAAGATTGT 
GTGGAAGGGA GATTTGGGAT TGGATGCTCT CAGTCTTGTG ACTGCTCTGG CGCACCATGT 
GACAAAGTGA CTGGTCAATG CACGTGTCCT GCTGGAACGT TTGGAAAGCG CTGTGAAAAA 
GAGTGTCCTG AGGGCCGCTG GGGTCTGGGC TGTCAGGAAT GGTGTCCGGT GTGCGAGAAC 
GGAGGCCGCT GTGACCGGCG TAACGGCACA TGTGTGTGTT CTCCTGGATT CATCGGCAGA 
TTGTGTCAGA ACTCATGTCC TGCGGGTCGC TTCGGTGTGG GCTGTCAGCT CAGATGTGTG 
TGTGATCTCG GAGTTCGCTG TAATCCTGTA AGCGGACGCT GCTCGTGTCC TCCAGGCTGG 
ACGGGACACA ACTGCAGGAA GGTGTGTGAC GCGGGCCGCT GGGGTCTGGA CTGTGAGAGT 
GTATGTGACT GTCGTAATGC TGACGGTGTG TGTGATGCTC AGACTGGACG CTGTCATTGT 
GAGGCCGGAT TTACTGGAGC ACGATGTGAC CAGAAATGCC CGGCTGGCTC GTTTGGGCCC 
GGCTGTAAGC ATCGCTGCCA GTGTGAAAAT CAGGCATCAT GTGACCACGT GGGCGGAGCT 
TGTACCTGTA AGACCGGCTG GACCGGAACC TTCTGTGAAA AACCCTGTCC GCAGGGTTTC 
TATGGTCTGG ACTGTCAGCA GAAGTGTGTG TGTTTGAACG GCGGTCTCTG TGATCATGTG 
AGCGGTCAGT GTTTCTGTAA GGCCGGATGG ATCGGGACGC GCTGTAACCA GACGTGTCCT 
GCGGGATGGT TTGGCGTGAG CTGTTCTCAG ACCTGTGTTT GTCACAATAA CAGCAGCTGT 
GATGCAGTGA GCGGTCAGTG TGAATGCGGT GCAGGCTGGA CAGGAGACAC ATGCTCACAG 
CGCTGTCCTG CAGGTGTGTT CGGGGACGGT TGTGTTCACA GGTGTTTGTG TCAGAACGGG 
GCCTCGTGTG ACCCTGAGAG TGGACACTGC TTCTGTCCTA GCGGCTGGAC GGGGGCCGCC 
TGTCAGCTTG AATGTCCAGC GGGGAGGTTC GGCTCAGACT GTCAGGACAG ATGTGAATGT 
GTGAATGGAG CTCAGTGTGA TGGTCAGACG GGTCGCTGTG TTTGTCCGCC GGGCTGGACA 
GGAGAGCGCT GTGAGAATGC ATGTCTGCCG GGCTCATTTG GTCAGGATTG TGTCCAGCGC 
TGCTCTTGTC CGTCCGGCTC CTCGTGTCAT CATGTGACCG GTCACTGTGG TTGTGCTCCA 
GGATTCACCG GCGACGGCTG TGAACACAGT TGTCTGCCTG GCACGTTCGG CCAGGACTGT 
AATCAGGTGT GTCAGTGTTC AGAAAGAAAC CAGCTCTGCC ACCCGGTGAC TGGAGTGTGT 
TACTGCGCGC CCGGATTCAC TGGACTCAAG TGTGAACAGA CGTGTCCGCA GGGTTTGTAC 
GGCTCAGGCT GTGAGCATCA GTGTGAGTGT CTGAATGGTG GAGTTTGTCT CTCAGACTCG 
GGATCCTGTC AGTGTCCAGC CGGATTCATT GGAGCTCAAT GCAATCAAAC ATGTCCAGCG 
GGTCGCTATG GTCTGGACTG TGCTCATGTG TCAGCGTGTG GGACAGGAGT GAGGAATGAT 
CCGGCTACAG GAAGATGCAC TGCACATCAA GTCTGTCCTC CAGGTTGGTT TGGTTCTGGC 
TGTTCTCAGC GTTGTGACTG CAGTAACAGA GGTGTGTGTG ACGGAGTCTC TGGAAACTGC 
TCATGTGCTC TCGGCTGGAC CGGCAAACAC TGTGATAAAG AGTGTCCTGA TGGCAGCTTT 
GGGCCTGACT GCTCTCTTCA GTGTTCCTGT CAGAATAACA GCACATGTGA CCGAGTGACA 
GGAAGTTGTC ATTGTCGTCC AGGATATTAC GGTCATCTGT GTGAACACGC GTGTCCGGCT 
GGTCTCTTCG GCTCTGGGTG TCAGCAGCAG TGTGACTGTA TGAACGGAGC CTCCTGTCAT 
CCGGATACAG GACAGTGTGT GTGTCCCGCT GGAGTCCACG GCCCGCGCTG TCAGCACGTG 
TGTGCTCAGG GCAGGTTTGG TGTGAACTGC TCTCAGTCAT GTGACTGTGC TGATGGAGCG 
TCATGTGACC CGGTCAGCGG CCGCTGTGTT TGTCCATCAG GGAAGACTGG AGTGCGGTGT 
GACAGCGACT GCAGTGTGAA CCGGTTTGGC CCTGACTGCT CTCTGCTGTG CGAGTGTGAA 
AATGGAGCTC AGTGCGACAG CAGAAATGGA CGCTGCTTCT GTCTGAGCGG CTGGATCGGC 
TTCACATGTT CACAGAGCGC TCCTCCAGAA CTGAACTCCA GACGGTCAGA AGAGGATCAG 
CTTCAGCTCA ACACTTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF6B ( XM_021479907.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021335582.1 Danio rerio multiple EGF-like-domains 6b (megf6b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa42666
Clone ID Related Accession (Same CDS sequence)	XM_021479907.1
Accession Version	XM_021479907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	multiple epidermal growth factor-like domains protein 6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACG TGGATTTATT ACTTTCCTTA ATCATTCTAG TTGATGGAAT TAATGCAGTA 
TCGCGTCTCG GGAAGGACCT GAAGCCGCAC ATGCCGAATG TGTGTGCAGA GAAGGAGGTG 
AAGCTGGTGG CTCAGATGCA GCCGTGTGTT CAAGCCTTCA CACGAATGGT TAAAGCATGG 
AAACAAGGTT GTCAGGGTCA GTCCTGGTGT GTGGGATACG AGAGGAGAAC AGCTTATTAT 
ACTGCATACA GACAGGTGTA TCGACAGGAA CAGCAGACGG TTTACAAGTG TTGTCCAGGA 
TGGTCACAGC TCAACGGAGA GGCCGGATGC CTGTACCCGG TCTGCAGGTC TGGTGTGTGT 
TTTAATGGCG GTCAGTGTAA AGAAGGTTCC GCTCAGCTCT GTGTGTGTCC AGCAGGATTC 
AGCGGCCCCA GCTGCCAATA TGATGTGAAC GAGTGTGAAG AGACCAATGG AGGCTGTGAG 
GCTTTATGCT GTAACACCAT CGGCAGCTTC TACTGCAAGT GTCCCGCAGG TCAAGAGCTC 
AAGGAGGATG GCAAGACCTG CCAAGATATT GACGAGTGCC AGGTTCATAA CGGCGGCTGC 
CAGCACACAT GTGTGAACAC TAGGGGTTCG TATCACTGCC AGTGTAACGC CGGCTCTCGA 
CTGCATGTGG ACGGACGCAC CTGCATCGCT GTTCAGTCCT GCGGCGTTGG GAATGGCGGC 
TGTGAGCATT TCTGTGTCCA GCAGACGGCG GGTCATGTCC AGTGTCGCTG CAGACCTGAT 
TACAGACTGG ACGCGGATGG AAAACACTGC ACATTGGTGA ACCCGTGTGC GGAGCAGAAT 
GGCGGATGTT CTCAGATGTG TCATAGGGAT GGAGCTCAGG CCCGCTGTGA ATGTCATCCA 
GGTTATCAGC TCGCTGAAGA CGGCAAGACA TGTGAAGACA TTGATGAGTG TGCGTTGGGT 
CAGACGGACT GTGCTCATGG CTGTCGTAAC ACCAGAGGAT CCTTCATGTG TGTGTGTTCT 
GCTGCCTATG AGCTCGGGGT GGACGGTAAA CAGTGCTATC GTATTGAGAT GGAGATCGTG 
AACAGCTGTG ATCATGATAA TGGTGGTTGT TCTCATCACT GTGAACATTC GACCGCTGGT 
CCGCTGTGCA GCTGTAATGA AGGATATCAG CTGGACCACG ACCACAAGAC CTGCGTCGAT 
ATAAACGAGT GTGTCGACGG CAGCTCGTGT TGTGAACATG ACTGTACGAA CTATCCTGGA 
GGATACGAGT GTTACTGTAC AGCCGGATAT CGACTGAACA CTGACGGATG CAGCTGTGAT 
GATATAGATG AGTGTTTGGC TGTTAATGGA GGATGTGATC ATGCTTGTCT GAACAGCGCC 
GGCTCTTTCC AGTGTTCCTG TAGACGTGGA TATCGACTCG ATGAGGACAG AAGATCCTGC 
ATACTGATGG AGGAGTCAGT GGAGGCGTTG AGCAGCGGTC AGACAGTCAA TAAACCTGGA 
CCAGGACCTC AGCTCACCAT CCTGCAGGAC TTCGATCAGA TCCTGGAGAA ATACGAGGAC 
TACGAGGACG ACACTGGAGA ACTGCGTGCC GAGAGCACTT TAGCTGAGAA ATTCATTTGT 
TTGAACGATT CGTTCGGCGT TGACTGCAGT CTGTCCTGTG AGGACTGTGT GAACGGAGGA 
GTGTGTAACA AACACAGAAA TGCCTGTGAC TGTCCTGACG GATGGACTGG AGTCGTCTGT 
AATGAGACAT GCCCTGAGGG TTGGTTTGGC AGGAATTGCT CACTGTTGTG TAAGTGTAAG 
AACGGTGGTG TGTGTGATCC GGTGTCCGGG AGCTGTCGCT GTCCACCAGG GGTCAGTGGA 
GAGCTGTGTC AGGACGGCTG TCCGAAGGGT CTCTATGGGA AGTTTTGCAA TAAAAGGTGT 
AACTGCGCTA ATAACGGACG TTGTCATCGC ACTTATGGAG CGTGTTTGTG TGACCCGGGA 
CTGTATGGAC GATTCTGCCA CCTTCCGTGT CCGAAGTGGA TGTTTGGTCC CGGTTGTTCA 
GAAGAGTGTC AGTGTGTCCA GCAGAACACT ATAGAGTGTC ACCGTCATCA TGGAGACTGC 
ATCTGCAAAC CTGGATACAG AGGCCACACC TGCAAAGACG AGTGTGAATC AGGGTTTTTT 
GGCTCTGCTT GTGAGAATAA ATGCTCTTGT CCGCCTGGAG TGACCTGTGA TCATGTGACT 
GGAGCTTGTC AACCCAAATG TCCAGCTGGA AAAACAGGCG ACAAATGTGA CCAAGATTGT 
GTGGAAGGGA GATTTGGGAT TGGATGCTCT CAGTCTTGTG ACTGCTCTGG CGCACCATGT 
GACAAAGTGA CTGGTCAATG CACGTGTCCT GCTGGAACGT TTGGAAAGCG CTGTGAAAAA 
GAGTGTCCTG AGGGCCGCTG GGGTCTGGGC TGTCAGGAAT GGTGTCCGGT GTGCGAGAAC 
GGAGGCCGCT GTGACCGGCG TAACGGCACA TGTGTGTGTT CTCCTGGATT CATCGGCAGA 
TTGTGTCAGA ACTCATGTCC TGCGGGTCGC TTCGGTGTGG GCTGTCAGCT CAGATGTGTG 
TGTGATCTCG GAGTTCGCTG TAATCCTGTA AGCGGACGCT GCTCGTGTCC TCCAGGCTGG 
ACGGGACACA ACTGCAGGAA GGTGTGTGAC GCGGGCCGCT GGGGTCTGGA CTGTGAGAGT 
GTATGTGACT GTCGTAATGC TGACGGTGTG TGTGATGCTC AGACTGGACG CTGTCATTGT 
GAGGCCGGAT TTACTGGAGC ACGATGTGAC CAGACGTGTC CTGCGGGATG GTTTGGCGTG 
AGCTGTTCTC AGACCTGTGT TTGTCACAAT AACAGCAGCT GTGATGCAGT GAGCGGTCAG 
TGTGAATGCG GTGCAGGCTG GACAGGAGAC ACATGCTCAC AGCGCTGTCC TGCAGGTGTG 
TTCGGGGACG GTTGTGTTCA CAGGTGTTTG TGTCAGAACG GGGCCTCGTG TGACCCTGAG 
AGTGGACACT GCTTCTGTCC TAGCGGCTGG ACGGGGGCCG CCTGTCAGCT TGAATGTCCA 
GCGGGGAGGT TCGGCTCAGA CTGTCAGGAC AGATGTGAAT GTGTGAATGG AGCTCAGTGT 
GATGGTCAGA CGGGTCGCTG TGTTTGTCCG CCGGGCTGGA CAGGAGAGCG CTGTGAGAAT 
GAGTGTGAAC GGGGCTGGTT TGGCGTGGGT TGTGAGGACC GCTGTCAGTG TGATCACGGT 
GCTGTCTGCC ATCACGTGAG CGGTCAGTGT CTCTGCCCTG CTGGATGGAG AGGAAGACGC 
TGTGAGAAAG CATGTCTGCC GGGCTCATTT GGTCAGGATT GTGTCCAGCG CTGCTCTTGT 
CCGTCCGGCT CCTCGTGTCA TCATGTGACC GGTCACTGTG GTTGTGCTCC AGGATTCACC 
GGCGACGGCT GTGAACACAG TTGTCTGCCT GGCACGTTCG GCCAGGACTG TAATCAGGTG 
TGTCAGTGTT CAGAAAGAAA CCAGCTCTGC CACCCGGTGA CTGGAGTGTG TTACTGCGCG 
CCCGGATTCA CTGGACTCAA GTGTGAACAG ACGTGTCCGC AGGGTTTGTA CGGCTCAGGC 
TGTGAGCATC AGTGTGAGTG TCTGAATGGT GGAGTTTGTC TCTCAGACTC GGGATCCTGT 
CAGTGTCCAG CCGGATTCAT TGGAGCTCAA TGCAATCAAA CATGTCCAGC GGGTCGCTAT 
GGTCTGGACT GTGCTCATGT GTCAGCGTGT GGGACAGGAG TGAGGAATGA TCCGGCTACA 
GGAAGATGCA CTGCACATCA AGTCTGTCCT CCAGGTTGGT TTGGTTCTGG CTGTTCTCAG 
CGTTGTGACT GCAGTAACAG AGGTGTGTGT GACGGAGTCT CTGGAAACTG CTCATGTGCT 
CTCGGCTGGA CCGGCAAACA CTGTGATAAA GAGTGTCCTG ATGGCAGCTT TGGGCCTGAC 
TGCTCTCTTC AGTGTTCCTG TCAGAATAAC AGCACATGTG ACCGAGTGAC AGGAAGTTGT 
CATTGTCGTC CAGGATATTA CGGTCATCTG TGTGAACACG CGTGTCCGGC TGGTCTCTTC 
GGCTCTGGGT GTCAGCAGCA GTGTGACTGT ATGAACGGAG CCTCCTGTCA TCCGGATACA 
GGACAGTGTG TGTGTCCCGC TGGAGTCCAC GGCCCGCGCT GTCAGCACGT GTGTGCTCAG 
GGCAGGTTTG GTGTGAACTG CTCTCAGTCA TGTGACTGTG CTGATGGAGC GTCATGTGAC 
CCGGTCAGCG GCCGCTGTGT TTGTCCATCA GGGAAGACTG GAGTGCGGTG TGACAGCGAC 
TGCAGTGTGA ACCGGTTTGG CCCTGACTGC TCTCTGCTGT GCGAGTGTGA AAATGGAGCT 
CAGTGCGACA GCAGAAATGG ACGCTGCTTC TGTCTGAGCG GCTGGATCGG CTTCACATGT 
TCACAGAGCG CTCCTCCAGA ACTGAACTCC AGACGGTCAG AAGAGGATCA GCTTCAGCTC 
AACACTTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335582.1
CDS	680..5191
Translation	MKNVDLLLSLIILVDGINAVSRLGKDLKPHMPNVCAEKEVKLVAQMQPCVQAFTRMVKAWKQGCQGQSWCVGYERRTAYYTAYRQVYRQEQQTVYKCCPGWSQLNGEAGCLYPVCRSGVCFNGGQCKEGSAQLCVCPAGFSGPSCQYDVNECEETNGGCEALCCNTIGSFYCKCPAGQELKEDGKTCQDIDECQVHNGGCQHTCVNTRGSYHCQCNAGSRLHVDGRTCIAVQSCGVGNGGCEHFCVQQTAGHVQCRCRPDYRLDADGKHCTLVNPCAEQNGGCSQMCHRDGAQARCECHPGYQLAEDGKTCEDIDECALGQTDCAHGCRNTRGSFMCVCSAAYELGVDGKQCYRIEMEIVNSCDHDNGGCSHHCEHSTAGPLCSCNEGYQLDHDHKTCVDINECVDGSSCCEHDCTNYPGGYECYCTAGYRLNTDGCSCDDIDECLAVNGGCDHACLNSAGSFQCSCRRGYRLDEDRRSCILMEESVEALSSGQTVNKPGPGPQLTILQDFDQILEKYEDYEDDTGELRAESTLAEKFICLNDSFGVDCSLSCEDCVNGGVCNKHRNACDCPDGWTGVVCNETCPEGWFGRNCSLLCKCKNGGVCDPVSGSCRCPPGVSGELCQDGCPKGLYGKFCNKRCNCANNGRCHRTYGACLCDPGLYGRFCHLPCPKWMFGPGCSEECQCVQQNTIECHRHHGDCICKPGYRGHTCKDECESGFFGSACENKCSCPPGVTCDHVTGACQPKCPAGKTGDKCDQDCVEGRFGIGCSQSCDCSGAPCDKVTGQCTCPAGTFGKRCEKECPEGRWGLGCQEWCPVCENGGRCDRRNGTCVCSPGFIGRLCQNSCPAGRFGVGCQLRCVCDLGVRCNPVSGRCSCPPGWTGHNCRKVCDAGRWGLDCESVCDCRNADGVCDAQTGRCHCEAGFTGARCDQTCPAGWFGVSCSQTCVCHNNSSCDAVSGQCECGAGWTGDTCSQRCPAGVFGDGCVHRCLCQNGASCDPESGHCFCPSGWTGAACQLECPAGRFGSDCQDRCECVNGAQCDGQTGRCVCPPGWTGERCENECERGWFGVGCEDRCQCDHGAVCHHVSGQCLCPAGWRGRRCEKACLPGSFGQDCVQRCSCPSGSSCHHVTGHCGCAPGFTGDGCEHSCLPGTFGQDCNQVCQCSERNQLCHPVTGVCYCAPGFTGLKCEQTCPQGLYGSGCEHQCECLNGGVCLSDSGSCQCPAGFIGAQCNQTCPAGRYGLDCAHVSACGTGVRNDPATGRCTAHQVCPPGWFGSGCSQRCDCSNRGVCDGVSGNCSCALGWTGKHCDKECPDGSFGPDCSLQCSCQNNSTCDRVTGSCHCRPGYYGHLCEHACPAGLFGSGCQQQCDCMNGASCHPDTGQCVCPAGVHGPRCQHVCAQGRFGVNCSQSCDCADGASCDPVSGRCVCPSGKTGVRCDSDCSVNRFGPDCSLLCECENGAQCDSRNGRCFCLSGWIGFTCSQSAPPELNSRRSEEDQLQLNTS

Target ORF information:

RefSeq Version	XM_021479907.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio multiple EGF-like-domains 6b (megf6b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479907.1

ORF Insert Sequence:

ATGAAGAACG TGGATTTATT ACTTTCCTTA ATCATTCTAG TTGATGGAAT TAATGCAGTA 
TCGCGTCTCG GGAAGGACCT GAAGCCGCAC ATGCCGAATG TGTGTGCAGA GAAGGAGGTG 
AAGCTGGTGG CTCAGATGCA GCCGTGTGTT CAAGCCTTCA CACGAATGGT TAAAGCATGG 
AAACAAGGTT GTCAGGGTCA GTCCTGGTGT GTGGGATACG AGAGGAGAAC AGCTTATTAT 
ACTGCATACA GACAGGTGTA TCGACAGGAA CAGCAGACGG TTTACAAGTG TTGTCCAGGA 
TGGTCACAGC TCAACGGAGA GGCCGGATGC CTGTACCCGG TCTGCAGGTC TGGTGTGTGT 
TTTAATGGCG GTCAGTGTAA AGAAGGTTCC GCTCAGCTCT GTGTGTGTCC AGCAGGATTC 
AGCGGCCCCA GCTGCCAATA TGATGTGAAC GAGTGTGAAG AGACCAATGG AGGCTGTGAG 
GCTTTATGCT GTAACACCAT CGGCAGCTTC TACTGCAAGT GTCCCGCAGG TCAAGAGCTC 
AAGGAGGATG GCAAGACCTG CCAAGATATT GACGAGTGCC AGGTTCATAA CGGCGGCTGC 
CAGCACACAT GTGTGAACAC TAGGGGTTCG TATCACTGCC AGTGTAACGC CGGCTCTCGA 
CTGCATGTGG ACGGACGCAC CTGCATCGCT GTTCAGTCCT GCGGCGTTGG GAATGGCGGC 
TGTGAGCATT TCTGTGTCCA GCAGACGGCG GGTCATGTCC AGTGTCGCTG CAGACCTGAT 
TACAGACTGG ACGCGGATGG AAAACACTGC ACATTGGTGA ACCCGTGTGC GGAGCAGAAT 
GGCGGATGTT CTCAGATGTG TCATAGGGAT GGAGCTCAGG CCCGCTGTGA ATGTCATCCA 
GGTTATCAGC TCGCTGAAGA CGGCAAGACA TGTGAAGACA TTGATGAGTG TGCGTTGGGT 
CAGACGGACT GTGCTCATGG CTGTCGTAAC ACCAGAGGAT CCTTCATGTG TGTGTGTTCT 
GCTGCCTATG AGCTCGGGGT GGACGGTAAA CAGTGCTATC GTATTGAGAT GGAGATCGTG 
AACAGCTGTG ATCATGATAA TGGTGGTTGT TCTCATCACT GTGAACATTC GACCGCTGGT 
CCGCTGTGCA GCTGTAATGA AGGATATCAG CTGGACCACG ACCACAAGAC CTGCGTCGAT 
ATAAACGAGT GTGTCGACGG CAGCTCGTGT TGTGAACATG ACTGTACGAA CTATCCTGGA 
GGATACGAGT GTTACTGTAC AGCCGGATAT CGACTGAACA CTGACGGATG CAGCTGTGAT 
GATATAGATG AGTGTTTGGC TGTTAATGGA GGATGTGATC ATGCTTGTCT GAACAGCGCC 
GGCTCTTTCC AGTGTTCCTG TAGACGTGGA TATCGACTCG ATGAGGACAG AAGATCCTGC 
ATACTGATGG AGGAGTCAGT GGAGGCGTTG AGCAGCGGTC AGACAGTCAA TAAACCTGGA 
CCAGGACCTC AGCTCACCAT CCTGCAGGAC TTCGATCAGA TCCTGGAGAA ATACGAGGAC 
TACGAGGACG ACACTGGAGA ACTGCGTGCC GAGAGCACTT TAGCTGAGAA ATTCATTTGT 
TTGAACGATT CGTTCGGCGT TGACTGCAGT CTGTCCTGTG AGGACTGTGT GAACGGAGGA 
GTGTGTAACA AACACAGAAA TGCCTGTGAC TGTCCTGACG GATGGACTGG AGTCGTCTGT 
AATGAGACAT GCCCTGAGGG TTGGTTTGGC AGGAATTGCT CACTGTTGTG TAAGTGTAAG 
AACGGTGGTG TGTGTGATCC GGTGTCCGGG AGCTGTCGCT GTCCACCAGG GGTCAGTGGA 
GAGCTGTGTC AGGACGGCTG TCCGAAGGGT CTCTATGGGA AGTTTTGCAA TAAAAGGTGT 
AACTGCGCTA ATAACGGACG TTGTCATCGC ACTTATGGAG CGTGTTTGTG TGACCCGGGA 
CTGTATGGAC GATTCTGCCA CCTTCCGTGT CCGAAGTGGA TGTTTGGTCC CGGTTGTTCA 
GAAGAGTGTC AGTGTGTCCA GCAGAACACT ATAGAGTGTC ACCGTCATCA TGGAGACTGC 
ATCTGCAAAC CTGGATACAG AGGCCACACC TGCAAAGACG AGTGTGAATC AGGGTTTTTT 
GGCTCTGCTT GTGAGAATAA ATGCTCTTGT CCGCCTGGAG TGACCTGTGA TCATGTGACT 
GGAGCTTGTC AACCCAAATG TCCAGCTGGA AAAACAGGCG ACAAATGTGA CCAAGATTGT 
GTGGAAGGGA GATTTGGGAT TGGATGCTCT CAGTCTTGTG ACTGCTCTGG CGCACCATGT 
GACAAAGTGA CTGGTCAATG CACGTGTCCT GCTGGAACGT TTGGAAAGCG CTGTGAAAAA 
GAGTGTCCTG AGGGCCGCTG GGGTCTGGGC TGTCAGGAAT GGTGTCCGGT GTGCGAGAAC 
GGAGGCCGCT GTGACCGGCG TAACGGCACA TGTGTGTGTT CTCCTGGATT CATCGGCAGA 
TTGTGTCAGA ACTCATGTCC TGCGGGTCGC TTCGGTGTGG GCTGTCAGCT CAGATGTGTG 
TGTGATCTCG GAGTTCGCTG TAATCCTGTA AGCGGACGCT GCTCGTGTCC TCCAGGCTGG 
ACGGGACACA ACTGCAGGAA GGTGTGTGAC GCGGGCCGCT GGGGTCTGGA CTGTGAGAGT 
GTATGTGACT GTCGTAATGC TGACGGTGTG TGTGATGCTC AGACTGGACG CTGTCATTGT 
GAGGCCGGAT TTACTGGAGC ACGATGTGAC CAGACGTGTC CTGCGGGATG GTTTGGCGTG 
AGCTGTTCTC AGACCTGTGT TTGTCACAAT AACAGCAGCT GTGATGCAGT GAGCGGTCAG 
TGTGAATGCG GTGCAGGCTG GACAGGAGAC ACATGCTCAC AGCGCTGTCC TGCAGGTGTG 
TTCGGGGACG GTTGTGTTCA CAGGTGTTTG TGTCAGAACG GGGCCTCGTG TGACCCTGAG 
AGTGGACACT GCTTCTGTCC TAGCGGCTGG ACGGGGGCCG CCTGTCAGCT TGAATGTCCA 
GCGGGGAGGT TCGGCTCAGA CTGTCAGGAC AGATGTGAAT GTGTGAATGG AGCTCAGTGT 
GATGGTCAGA CGGGTCGCTG TGTTTGTCCG CCGGGCTGGA CAGGAGAGCG CTGTGAGAAT 
GAGTGTGAAC GGGGCTGGTT TGGCGTGGGT TGTGAGGACC GCTGTCAGTG TGATCACGGT 
GCTGTCTGCC ATCACGTGAG CGGTCAGTGT CTCTGCCCTG CTGGATGGAG AGGAAGACGC 
TGTGAGAAAG CATGTCTGCC GGGCTCATTT GGTCAGGATT GTGTCCAGCG CTGCTCTTGT 
CCGTCCGGCT CCTCGTGTCA TCATGTGACC GGTCACTGTG GTTGTGCTCC AGGATTCACC 
GGCGACGGCT GTGAACACAG TTGTCTGCCT GGCACGTTCG GCCAGGACTG TAATCAGGTG 
TGTCAGTGTT CAGAAAGAAA CCAGCTCTGC CACCCGGTGA CTGGAGTGTG TTACTGCGCG 
CCCGGATTCA CTGGACTCAA GTGTGAACAG ACGTGTCCGC AGGGTTTGTA CGGCTCAGGC 
TGTGAGCATC AGTGTGAGTG TCTGAATGGT GGAGTTTGTC TCTCAGACTC GGGATCCTGT 
CAGTGTCCAG CCGGATTCAT TGGAGCTCAA TGCAATCAAA CATGTCCAGC GGGTCGCTAT 
GGTCTGGACT GTGCTCATGT GTCAGCGTGT GGGACAGGAG TGAGGAATGA TCCGGCTACA 
GGAAGATGCA CTGCACATCA AGTCTGTCCT CCAGGTTGGT TTGGTTCTGG CTGTTCTCAG 
CGTTGTGACT GCAGTAACAG AGGTGTGTGT GACGGAGTCT CTGGAAACTG CTCATGTGCT 
CTCGGCTGGA CCGGCAAACA CTGTGATAAA GAGTGTCCTG ATGGCAGCTT TGGGCCTGAC 
TGCTCTCTTC AGTGTTCCTG TCAGAATAAC AGCACATGTG ACCGAGTGAC AGGAAGTTGT 
CATTGTCGTC CAGGATATTA CGGTCATCTG TGTGAACACG CGTGTCCGGC TGGTCTCTTC 
GGCTCTGGGT GTCAGCAGCA GTGTGACTGT ATGAACGGAG CCTCCTGTCA TCCGGATACA 
GGACAGTGTG TGTGTCCCGC TGGAGTCCAC GGCCCGCGCT GTCAGCACGT GTGTGCTCAG 
GGCAGGTTTG GTGTGAACTG CTCTCAGTCA TGTGACTGTG CTGATGGAGC GTCATGTGAC 
CCGGTCAGCG GCCGCTGTGT TTGTCCATCA GGGAAGACTG GAGTGCGGTG TGACAGCGAC 
TGCAGTGTGA ACCGGTTTGG CCCTGACTGC TCTCTGCTGT GCGAGTGTGA AAATGGAGCT 
CAGTGCGACA GCAGAAATGG ACGCTGCTTC TGTCTGAGCG GCTGGATCGG CTTCACATGT 
TCACAGAGCG CTCCTCCAGA ACTGAACTCC AGACGGTCAG AAGAGGATCA GCTTCAGCTC 
AACACTTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF6B ( XM_009306294.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304569.1 Danio rerio multiple EGF-like-domains 6b (megf6b), transcript variant X1, mRNA.

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CloneID	ODa42664
Clone ID Related Accession (Same CDS sequence)	XM_009306294.3 , XM_009306294.2
Accession Version	XM_009306294.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	multiple epidermal growth factor-like domains protein 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306294.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACG TGGATTTATT ACTTTCCTTA ATCATTCTAG TTGATGGAAT TAATGCAGTA 
TCGCGTCTCG GGAAGGACCT GAAGCCGCAC ATGCCGAATG TGTGTGCAGA GAAGGAGGTG 
AAGCTGGTGG CTCAGATGCA GCCGTGTGTT CAAGCCTTCA CACGAATGGT TAAAGCATGG 
AAACAAGGTT GTCAGGGTCA GTCCTGGTGT GTGGGATACG AGAGGAGAAC AGCTTATTAT 
ACTGCATACA GACAGGTGTA TCGACAGGAA CAGCAGACGG TTTACAAGTG TTGTCCAGGA 
TGGTCACAGC TCAACGGAGA GGCCGGATGC CTGTACCCGG TCTGCAGGTC TGGTGTGTGT 
TTTAATGGCG GTCAGTGTAA AGAAGGTTCC GCTCAGCTCT GTGTGTGTCC AGCAGGATTC 
AGCGGCCCCA GCTGCCAATA TGATGTGAAC GAGTGTGAAG AGACCAATGG AGGCTGTGAG 
GCTTTATGCT GTAACACCAT CGGCAGCTTC TACTGCAAGT GTCCCGCAGG TCAAGAGCTC 
AAGGAGGATG GCAAGACCTG CCAAGATATT GACGAGTGCC AGGTTCATAA CGGCGGCTGC 
CAGCACACAT GTGTGAACAC TAGGGGTTCG TATCACTGCC AGTGTAACGC CGGCTCTCGA 
CTGCATGTGG ACGGACGCAC CTGCATCGCT GTTCAGTCCT GCGGCGTTGG GAATGGCGGC 
TGTGAGCATT TCTGTGTCCA GCAGACGGCG GGTCATGTCC AGTGTCGCTG CAGACCTGAT 
TACAGACTGG ACGCGGATGG AAAACACTGC ACATTGGTGA ACCCGTGTGC GGAGCAGAAT 
GGCGGATGTT CTCAGATGTG TCATAGGGAT GGAGCTCAGG CCCGCTGTGA ATGTCATCCA 
GGTTATCAGC TCGCTGAAGA CGGCAAGACA TGTGAAGACA TTGATGAGTG TGCGTTGGGT 
CAGACGGACT GTGCTCATGG CTGTCGTAAC ACCAGAGGAT CCTTCATGTG TGTGTGTTCT 
GCTGCCTATG AGCTCGGGGT GGACGGTAAA CAGTGCTATC GTATTGAGAT GGAGATCGTG 
AACAGCTGTG ATCATGATAA TGGTGGTTGT TCTCATCACT GTGAACATTC GACCGCTGGT 
CCGCTGTGCA GCTGTAATGA AGGATATCAG CTGGACCACG ACCACAAGAC CTGCGTCGAT 
ATAAACGAGT GTGTCGACGG CAGCTCGTGT TGTGAACATG ACTGTACGAA CTATCCTGGA 
GGATACGAGT GTTACTGTAC AGCCGGATAT CGACTGAACA CTGACGGATG CAGCTGTGAT 
GATATAGATG AGTGTTTGGC TGTTAATGGA GGATGTGATC ATGCTTGTCT GAACAGCGCC 
GGCTCTTTCC AGTGTTCCTG TAGACGTGGA TATCGACTCG ATGAGGACAG AAGATCCTGC 
ATACTGATGG AGGAGTCAGT GGAGGCGTTG AGCAGCGGTC AGACAGTCAA TAAACCTGGA 
CCAGGACCTC AGCTCACCAT CCTGCAGGAC TTCGATCAGA TCCTGGAGAA ATACGAGGAC 
TACGAGGACG ACACTGGAGA ACTGCGTGCC GAGAGCACTT TAGCTGAGAA ATTCATTTGT 
TTGAACGATT CGTTCGGCGT TGACTGCAGT CTGTCCTGTG AGGACTGTGT GAACGGAGGA 
GTGTGTAACA AACACAGAAA TGCCTGTGAC TGTCCTGACG GATGGACTGG AGTCGTCTGT 
AATGAGACAT GCCCTGAGGG TTGGTTTGGC AGGAATTGCT CACTGTTGTG TAAGTGTAAG 
AACGGTGGTG TGTGTGATCC GGTGTCCGGG AGCTGTCGCT GTCCACCAGG GGTCAGTGGA 
GAGCTGTGTC AGGACGGCTG TCCGAAGGGT CTCTATGGGA AGTTTTGCAA TAAAAGGTGT 
AACTGCGCTA ATAACGGACG TTGTCATCGC ACTTATGGAG CGTGTTTGTG TGACCCGGGA 
CTGTATGGAC GATTCTGCCA CCTTCCGTGT CCGAAGTGGA TGTTTGGTCC CGGTTGTTCA 
GAAGAGTGTC AGTGTGTCCA GCAGAACACT ATAGAGTGTC ACCGTCATCA TGGAGACTGC 
ATCTGCAAAC CTGGATACAG AGGCCACACC TGCAAAGACG AGTGTGAATC AGGGTTTTTT 
GGCTCTGCTT GTGAGAATAA ATGCTCTTGT CCGCCTGGAG TGACCTGTGA TCATGTGACT 
GGAGCTTGTC AACCCAAATG TCCAGCTGGA AAAACAGGCG ACAAATGTGA CCAAGATTGT 
GTGGAAGGGA GATTTGGGAT TGGATGCTCT CAGTCTTGTG ACTGCTCTGG CGCACCATGT 
GACAAAGTGA CTGGTCAATG CACGTGTCCT GCTGGAACGT TTGGAAAGCG CTGTGAAAAA 
GAGTGTCCTG AGGGCCGCTG GGGTCTGGGC TGTCAGGAAT GGTGTCCGGT GTGCGAGAAC 
GGAGGCCGCT GTGACCGGCG TAACGGCACA TGTGTGTGTT CTCCTGGATT CATCGGCAGA 
TTGTGTCAGA ACTCATGTCC TGCGGGTCGC TTCGGTGTGG GCTGTCAGCT CAGATGTGTG 
TGTGATCTCG GAGTTCGCTG TAATCCTGTA AGCGGACGCT GCTCGTGTCC TCCAGGCTGG 
ACGGGACACA ACTGCAGGAA GGTGTGTGAC GCGGGCCGCT GGGGTCTGGA CTGTGAGAGT 
GTATGTGACT GTCGTAATGC TGACGGTGTG TGTGATGCTC AGACTGGACG CTGTCATTGT 
GAGGCCGGAT TTACTGGAGC ACGATGTGAC CAGAAATGCC CGGCTGGCTC GTTTGGGCCC 
GGCTGTAAGC ATCGCTGCCA GTGTGAAAAT CAGGCATCAT GTGACCACGT GGGCGGAGCT 
TGTACCTGTA AGACCGGCTG GACCGGAACC TTCTGTGAAA AACCCTGTCC GCAGGGTTTC 
TATGGTCTGG ACTGTCAGCA GAAGTGTGTG TGTTTGAACG GCGGTCTCTG TGATCATGTG 
AGCGGTCAGT GTTTCTGTAA GGCCGGATGG ATCGGGACGC GCTGTAACCA GACGTGTCCT 
GCGGGATGGT TTGGCGTGAG CTGTTCTCAG ACCTGTGTTT GTCACAATAA CAGCAGCTGT 
GATGCAGTGA GCGGTCAGTG TGAATGCGGT GCAGGCTGGA CAGGAGACAC ATGCTCACAG 
CGCTGTCCTG CAGGTGTGTT CGGGGACGGT TGTGTTCACA GGTGTTTGTG TCAGAACGGG 
GCCTCGTGTG ACCCTGAGAG TGGACACTGC TTCTGTCCTA GCGGCTGGAC GGGGGCCGCC 
TGTCAGCTTG AATGTCCAGC GGGGAGGTTC GGCTCAGACT GTCAGGACAG ATGTGAATGT 
GTGAATGGAG CTCAGTGTGA TGGTCAGACG GGTCGCTGTG TTTGTCCGCC GGGCTGGACA 
GGAGAGCGCT GTGAGAATGA GTGTGAACGG GGCTGGTTTG GCGTGGGTTG TGAGGACCGC 
TGTCAGTGTG ATCACGGTGC TGTCTGCCAT CACGTGAGCG GTCAGTGTCT CTGCCCTGCT 
GGATGGAGAG GAAGACGCTG TGAGAAAGCA TGTCTGCCGG GCTCATTTGG TCAGGATTGT 
GTCCAGCGCT GCTCTTGTCC GTCCGGCTCC TCGTGTCATC ATGTGACCGG TCACTGTGGT 
TGTGCTCCAG GATTCACCGG CGACGGCTGT GAACACAGTT GTCTGCCTGG CACGTTCGGC 
CAGGACTGTA ATCAGGTGTG TCAGTGTTCA GAAAGAAACC AGCTCTGCCA CCCGGTGACT 
GGAGTGTGTT ACTGCGCGCC CGGATTCACT GGACTCAAGT GTGAACAGAC GTGTCCGCAG 
GGTTTGTACG GCTCAGGCTG TGAGCATCAG TGTGAGTGTC TGAATGGTGG AGTTTGTCTC 
TCAGACTCGG GATCCTGTCA GTGTCCAGCC GGATTCATTG GAGCTCAATG CAATCAAACA 
TGTCCAGCGG GTCGCTATGG TCTGGACTGT GCTCATGTGT CAGCGTGTGG GACAGGAGTG 
AGGAATGATC CGGCTACAGG AAGATGCACT GCACATCAAG TCTGTCCTCC AGGTTGGTTT 
GGTTCTGGCT GTTCTCAGCG TTGTGACTGC AGTAACAGAG GTGTGTGTGA CGGAGTCTCT 
GGAAACTGCT CATGTGCTCT CGGCTGGACC GGCAAACACT GTGATAAAGA GTGTCCTGAT 
GGCAGCTTTG GGCCTGACTG CTCTCTTCAG TGTTCCTGTC AGAATAACAG CACATGTGAC 
CGAGTGACAG GAAGTTGTCA TTGTCGTCCA GGATATTACG GTCATCTGTG TGAACACGCG 
TGTCCGGCTG GTCTCTTCGG CTCTGGGTGT CAGCAGCAGT GTGACTGTAT GAACGGAGCC 
TCCTGTCATC CGGATACAGG ACAGTGTGTG TGTCCCGCTG GAGTCCACGG CCCGCGCTGT 
CAGCACGTGT GTGCTCAGGG CAGGTTTGGT GTGAACTGCT CTCAGTCATG TGACTGTGCT 
GATGGAGCGT CATGTGACCC GGTCAGCGGC CGCTGTGTTT GTCCATCAGG GAAGACTGGA 
GTGCGGTGTG ACAGCGACTG CAGTGTGAAC CGGTTTGGCC CTGACTGCTC TCTGCTGTGC 
GAGTGTGAAA ATGGAGCTCA GTGCGACAGC AGAAATGGAC GCTGCTTCTG TCTGAGCGGC 
TGGATCGGCT TCACATGTTC ACAGAGCGCT CCTCCAGAAC TGAACTCCAG ACGGTCAGAA 
GAGGATCAGC TTCAGCTCAA CACTTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304569.1
CDS	679..5448
Translation	MKNVDLLLSLIILVDGINAVSRLGKDLKPHMPNVCAEKEVKLVAQMQPCVQAFTRMVKAWKQGCQGQSWCVGYERRTAYYTAYRQVYRQEQQTVYKCCPGWSQLNGEAGCLYPVCRSGVCFNGGQCKEGSAQLCVCPAGFSGPSCQYDVNECEETNGGCEALCCNTIGSFYCKCPAGQELKEDGKTCQDIDECQVHNGGCQHTCVNTRGSYHCQCNAGSRLHVDGRTCIAVQSCGVGNGGCEHFCVQQTAGHVQCRCRPDYRLDADGKHCTLVNPCAEQNGGCSQMCHRDGAQARCECHPGYQLAEDGKTCEDIDECALGQTDCAHGCRNTRGSFMCVCSAAYELGVDGKQCYRIEMEIVNSCDHDNGGCSHHCEHSTAGPLCSCNEGYQLDHDHKTCVDINECVDGSSCCEHDCTNYPGGYECYCTAGYRLNTDGCSCDDIDECLAVNGGCDHACLNSAGSFQCSCRRGYRLDEDRRSCILMEESVEALSSGQTVNKPGPGPQLTILQDFDQILEKYEDYEDDTGELRAESTLAEKFICLNDSFGVDCSLSCEDCVNGGVCNKHRNACDCPDGWTGVVCNETCPEGWFGRNCSLLCKCKNGGVCDPVSGSCRCPPGVSGELCQDGCPKGLYGKFCNKRCNCANNGRCHRTYGACLCDPGLYGRFCHLPCPKWMFGPGCSEECQCVQQNTIECHRHHGDCICKPGYRGHTCKDECESGFFGSACENKCSCPPGVTCDHVTGACQPKCPAGKTGDKCDQDCVEGRFGIGCSQSCDCSGAPCDKVTGQCTCPAGTFGKRCEKECPEGRWGLGCQEWCPVCENGGRCDRRNGTCVCSPGFIGRLCQNSCPAGRFGVGCQLRCVCDLGVRCNPVSGRCSCPPGWTGHNCRKVCDAGRWGLDCESVCDCRNADGVCDAQTGRCHCEAGFTGARCDQKCPAGSFGPGCKHRCQCENQASCDHVGGACTCKTGWTGTFCEKPCPQGFYGLDCQQKCVCLNGGLCDHVSGQCFCKAGWIGTRCNQTCPAGWFGVSCSQTCVCHNNSSCDAVSGQCECGAGWTGDTCSQRCPAGVFGDGCVHRCLCQNGASCDPESGHCFCPSGWTGAACQLECPAGRFGSDCQDRCECVNGAQCDGQTGRCVCPPGWTGERCENECERGWFGVGCEDRCQCDHGAVCHHVSGQCLCPAGWRGRRCEKACLPGSFGQDCVQRCSCPSGSSCHHVTGHCGCAPGFTGDGCEHSCLPGTFGQDCNQVCQCSERNQLCHPVTGVCYCAPGFTGLKCEQTCPQGLYGSGCEHQCECLNGGVCLSDSGSCQCPAGFIGAQCNQTCPAGRYGLDCAHVSACGTGVRNDPATGRCTAHQVCPPGWFGSGCSQRCDCSNRGVCDGVSGNCSCALGWTGKHCDKECPDGSFGPDCSLQCSCQNNSTCDRVTGSCHCRPGYYGHLCEHACPAGLFGSGCQQQCDCMNGASCHPDTGQCVCPAGVHGPRCQHVCAQGRFGVNCSQSCDCADGASCDPVSGRCVCPSGKTGVRCDSDCSVNRFGPDCSLLCECENGAQCDSRNGRCFCLSGWIGFTCSQSAPPELNSRRSEEDQLQLNTS

Target ORF information:

RefSeq Version	XM_009306294.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio multiple EGF-like-domains 6b (megf6b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306294.3

ORF Insert Sequence:

ATGAAGAACG TGGATTTATT ACTTTCCTTA ATCATTCTAG TTGATGGAAT TAATGCAGTA 
TCGCGTCTCG GGAAGGACCT GAAGCCGCAC ATGCCGAATG TGTGTGCAGA GAAGGAGGTG 
AAGCTGGTGG CTCAGATGCA GCCGTGTGTT CAAGCCTTCA CACGAATGGT TAAAGCATGG 
AAACAAGGTT GTCAGGGTCA GTCCTGGTGT GTGGGATACG AGAGGAGAAC AGCTTATTAT 
ACTGCATACA GACAGGTGTA TCGACAGGAA CAGCAGACGG TTTACAAGTG TTGTCCAGGA 
TGGTCACAGC TCAACGGAGA GGCCGGATGC CTGTACCCGG TCTGCAGGTC TGGTGTGTGT 
TTTAATGGCG GTCAGTGTAA AGAAGGTTCC GCTCAGCTCT GTGTGTGTCC AGCAGGATTC 
AGCGGCCCCA GCTGCCAATA TGATGTGAAC GAGTGTGAAG AGACCAATGG AGGCTGTGAG 
GCTTTATGCT GTAACACCAT CGGCAGCTTC TACTGCAAGT GTCCCGCAGG TCAAGAGCTC 
AAGGAGGATG GCAAGACCTG CCAAGATATT GACGAGTGCC AGGTTCATAA CGGCGGCTGC 
CAGCACACAT GTGTGAACAC TAGGGGTTCG TATCACTGCC AGTGTAACGC CGGCTCTCGA 
CTGCATGTGG ACGGACGCAC CTGCATCGCT GTTCAGTCCT GCGGCGTTGG GAATGGCGGC 
TGTGAGCATT TCTGTGTCCA GCAGACGGCG GGTCATGTCC AGTGTCGCTG CAGACCTGAT 
TACAGACTGG ACGCGGATGG AAAACACTGC ACATTGGTGA ACCCGTGTGC GGAGCAGAAT 
GGCGGATGTT CTCAGATGTG TCATAGGGAT GGAGCTCAGG CCCGCTGTGA ATGTCATCCA 
GGTTATCAGC TCGCTGAAGA CGGCAAGACA TGTGAAGACA TTGATGAGTG TGCGTTGGGT 
CAGACGGACT GTGCTCATGG CTGTCGTAAC ACCAGAGGAT CCTTCATGTG TGTGTGTTCT 
GCTGCCTATG AGCTCGGGGT GGACGGTAAA CAGTGCTATC GTATTGAGAT GGAGATCGTG 
AACAGCTGTG ATCATGATAA TGGTGGTTGT TCTCATCACT GTGAACATTC GACCGCTGGT 
CCGCTGTGCA GCTGTAATGA AGGATATCAG CTGGACCACG ACCACAAGAC CTGCGTCGAT 
ATAAACGAGT GTGTCGACGG CAGCTCGTGT TGTGAACATG ACTGTACGAA CTATCCTGGA 
GGATACGAGT GTTACTGTAC AGCCGGATAT CGACTGAACA CTGACGGATG CAGCTGTGAT 
GATATAGATG AGTGTTTGGC TGTTAATGGA GGATGTGATC ATGCTTGTCT GAACAGCGCC 
GGCTCTTTCC AGTGTTCCTG TAGACGTGGA TATCGACTCG ATGAGGACAG AAGATCCTGC 
ATACTGATGG AGGAGTCAGT GGAGGCGTTG AGCAGCGGTC AGACAGTCAA TAAACCTGGA 
CCAGGACCTC AGCTCACCAT CCTGCAGGAC TTCGATCAGA TCCTGGAGAA ATACGAGGAC 
TACGAGGACG ACACTGGAGA ACTGCGTGCC GAGAGCACTT TAGCTGAGAA ATTCATTTGT 
TTGAACGATT CGTTCGGCGT TGACTGCAGT CTGTCCTGTG AGGACTGTGT GAACGGAGGA 
GTGTGTAACA AACACAGAAA TGCCTGTGAC TGTCCTGACG GATGGACTGG AGTCGTCTGT 
AATGAGACAT GCCCTGAGGG TTGGTTTGGC AGGAATTGCT CACTGTTGTG TAAGTGTAAG 
AACGGTGGTG TGTGTGATCC GGTGTCCGGG AGCTGTCGCT GTCCACCAGG GGTCAGTGGA 
GAGCTGTGTC AGGACGGCTG TCCGAAGGGT CTCTATGGGA AGTTTTGCAA TAAAAGGTGT 
AACTGCGCTA ATAACGGACG TTGTCATCGC ACTTATGGAG CGTGTTTGTG TGACCCGGGA 
CTGTATGGAC GATTCTGCCA CCTTCCGTGT CCGAAGTGGA TGTTTGGTCC CGGTTGTTCA 
GAAGAGTGTC AGTGTGTCCA GCAGAACACT ATAGAGTGTC ACCGTCATCA TGGAGACTGC 
ATCTGCAAAC CTGGATACAG AGGCCACACC TGCAAAGACG AGTGTGAATC AGGGTTTTTT 
GGCTCTGCTT GTGAGAATAA ATGCTCTTGT CCGCCTGGAG TGACCTGTGA TCATGTGACT 
GGAGCTTGTC AACCCAAATG TCCAGCTGGA AAAACAGGCG ACAAATGTGA CCAAGATTGT 
GTGGAAGGGA GATTTGGGAT TGGATGCTCT CAGTCTTGTG ACTGCTCTGG CGCACCATGT 
GACAAAGTGA CTGGTCAATG CACGTGTCCT GCTGGAACGT TTGGAAAGCG CTGTGAAAAA 
GAGTGTCCTG AGGGCCGCTG GGGTCTGGGC TGTCAGGAAT GGTGTCCGGT GTGCGAGAAC 
GGAGGCCGCT GTGACCGGCG TAACGGCACA TGTGTGTGTT CTCCTGGATT CATCGGCAGA 
TTGTGTCAGA ACTCATGTCC TGCGGGTCGC TTCGGTGTGG GCTGTCAGCT CAGATGTGTG 
TGTGATCTCG GAGTTCGCTG TAATCCTGTA AGCGGACGCT GCTCGTGTCC TCCAGGCTGG 
ACGGGACACA ACTGCAGGAA GGTGTGTGAC GCGGGCCGCT GGGGTCTGGA CTGTGAGAGT 
GTATGTGACT GTCGTAATGC TGACGGTGTG TGTGATGCTC AGACTGGACG CTGTCATTGT 
GAGGCCGGAT TTACTGGAGC ACGATGTGAC CAGAAATGCC CGGCTGGCTC GTTTGGGCCC 
GGCTGTAAGC ATCGCTGCCA GTGTGAAAAT CAGGCATCAT GTGACCACGT GGGCGGAGCT 
TGTACCTGTA AGACCGGCTG GACCGGAACC TTCTGTGAAA AACCCTGTCC GCAGGGTTTC 
TATGGTCTGG ACTGTCAGCA GAAGTGTGTG TGTTTGAACG GCGGTCTCTG TGATCATGTG 
AGCGGTCAGT GTTTCTGTAA GGCCGGATGG ATCGGGACGC GCTGTAACCA GACGTGTCCT 
GCGGGATGGT TTGGCGTGAG CTGTTCTCAG ACCTGTGTTT GTCACAATAA CAGCAGCTGT 
GATGCAGTGA GCGGTCAGTG TGAATGCGGT GCAGGCTGGA CAGGAGACAC ATGCTCACAG 
CGCTGTCCTG CAGGTGTGTT CGGGGACGGT TGTGTTCACA GGTGTTTGTG TCAGAACGGG 
GCCTCGTGTG ACCCTGAGAG TGGACACTGC TTCTGTCCTA GCGGCTGGAC GGGGGCCGCC 
TGTCAGCTTG AATGTCCAGC GGGGAGGTTC GGCTCAGACT GTCAGGACAG ATGTGAATGT 
GTGAATGGAG CTCAGTGTGA TGGTCAGACG GGTCGCTGTG TTTGTCCGCC GGGCTGGACA 
GGAGAGCGCT GTGAGAATGA GTGTGAACGG GGCTGGTTTG GCGTGGGTTG TGAGGACCGC 
TGTCAGTGTG ATCACGGTGC TGTCTGCCAT CACGTGAGCG GTCAGTGTCT CTGCCCTGCT 
GGATGGAGAG GAAGACGCTG TGAGAAAGCA TGTCTGCCGG GCTCATTTGG TCAGGATTGT 
GTCCAGCGCT GCTCTTGTCC GTCCGGCTCC TCGTGTCATC ATGTGACCGG TCACTGTGGT 
TGTGCTCCAG GATTCACCGG CGACGGCTGT GAACACAGTT GTCTGCCTGG CACGTTCGGC 
CAGGACTGTA ATCAGGTGTG TCAGTGTTCA GAAAGAAACC AGCTCTGCCA CCCGGTGACT 
GGAGTGTGTT ACTGCGCGCC CGGATTCACT GGACTCAAGT GTGAACAGAC GTGTCCGCAG 
GGTTTGTACG GCTCAGGCTG TGAGCATCAG TGTGAGTGTC TGAATGGTGG AGTTTGTCTC 
TCAGACTCGG GATCCTGTCA GTGTCCAGCC GGATTCATTG GAGCTCAATG CAATCAAACA 
TGTCCAGCGG GTCGCTATGG TCTGGACTGT GCTCATGTGT CAGCGTGTGG GACAGGAGTG 
AGGAATGATC CGGCTACAGG AAGATGCACT GCACATCAAG TCTGTCCTCC AGGTTGGTTT 
GGTTCTGGCT GTTCTCAGCG TTGTGACTGC AGTAACAGAG GTGTGTGTGA CGGAGTCTCT 
GGAAACTGCT CATGTGCTCT CGGCTGGACC GGCAAACACT GTGATAAAGA GTGTCCTGAT 
GGCAGCTTTG GGCCTGACTG CTCTCTTCAG TGTTCCTGTC AGAATAACAG CACATGTGAC 
CGAGTGACAG GAAGTTGTCA TTGTCGTCCA GGATATTACG GTCATCTGTG TGAACACGCG 
TGTCCGGCTG GTCTCTTCGG CTCTGGGTGT CAGCAGCAGT GTGACTGTAT GAACGGAGCC 
TCCTGTCATC CGGATACAGG ACAGTGTGTG TGTCCCGCTG GAGTCCACGG CCCGCGCTGT 
CAGCACGTGT GTGCTCAGGG CAGGTTTGGT GTGAACTGCT CTCAGTCATG TGACTGTGCT 
GATGGAGCGT CATGTGACCC GGTCAGCGGC CGCTGTGTTT GTCCATCAGG GAAGACTGGA 
GTGCGGTGTG ACAGCGACTG CAGTGTGAAC CGGTTTGGCC CTGACTGCTC TCTGCTGTGC 
GAGTGTGAAA ATGGAGCTCA GTGCGACAGC AGAAATGGAC GCTGCTTCTG TCTGAGCGGC 
TGGATCGGCT TCACATGTTC ACAGAGCGCT CCTCCAGAAC TGAACTCCAG ACGGTCAGAA 
GAGGATCAGC TTCAGCTCAA CACTTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF6B ( XM_009306294.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304569.1 Danio rerio multiple EGF-like-domains 6b (megf6b), transcript variant X1, mRNA.

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CloneID	ODa42664
Clone ID Related Accession (Same CDS sequence)	XM_009306294.3 , XM_009306294.2
Accession Version	XM_009306294.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	multiple epidermal growth factor-like domains protein 6 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306294.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACG TGGATTTATT ACTTTCCTTA ATCATTCTAG TTGATGGAAT TAATGCAGTA 
TCGCGTCTCG GGAAGGACCT GAAGCCGCAC ATGCCGAATG TGTGTGCAGA GAAGGAGGTG 
AAGCTGGTGG CTCAGATGCA GCCGTGTGTT CAAGCCTTCA CACGAATGGT TAAAGCATGG 
AAACAAGGTT GTCAGGGTCA GTCCTGGTGT GTGGGATACG AGAGGAGAAC AGCTTATTAT 
ACTGCATACA GACAGGTGTA TCGACAGGAA CAGCAGACGG TTTACAAGTG TTGTCCAGGA 
TGGTCACAGC TCAACGGAGA GGCCGGATGC CTGTACCCGG TCTGCAGGTC TGGTGTGTGT 
TTTAATGGCG GTCAGTGTAA AGAAGGTTCC GCTCAGCTCT GTGTGTGTCC AGCAGGATTC 
AGCGGCCCCA GCTGCCAATA TGATGTGAAC GAGTGTGAAG AGACCAATGG AGGCTGTGAG 
GCTTTATGCT GTAACACCAT CGGCAGCTTC TACTGCAAGT GTCCCGCAGG TCAAGAGCTC 
AAGGAGGATG GCAAGACCTG CCAAGATATT GACGAGTGCC AGGTTCATAA CGGCGGCTGC 
CAGCACACAT GTGTGAACAC TAGGGGTTCG TATCACTGCC AGTGTAACGC CGGCTCTCGA 
CTGCATGTGG ACGGACGCAC CTGCATCGCT GTTCAGTCCT GCGGCGTTGG GAATGGCGGC 
TGTGAGCATT TCTGTGTCCA GCAGACGGCG GGTCATGTCC AGTGTCGCTG CAGACCTGAT 
TACAGACTGG ACGCGGATGG AAAACACTGC ACATTGGTGA ACCCGTGTGC GGAGCAGAAT 
GGCGGATGTT CTCAGATGTG TCATAGGGAT GGAGCTCAGG CCCGCTGTGA ATGTCATCCA 
GGTTATCAGC TCGCTGAAGA CGGCAAGACA TGTGAAGACA TTGATGAGTG TGCGTTGGGT 
CAGACGGACT GTGCTCATGG CTGTCGTAAC ACCAGAGGAT CCTTCATGTG TGTGTGTTCT 
GCTGCCTATG AGCTCGGGGT GGACGGTAAA CAGTGCTATC GTATTGAGAT GGAGATCGTG 
AACAGCTGTG ATCATGATAA TGGTGGTTGT TCTCATCACT GTGAACATTC GACCGCTGGT 
CCGCTGTGCA GCTGTAATGA AGGATATCAG CTGGACCACG ACCACAAGAC CTGCGTCGAT 
ATAAACGAGT GTGTCGACGG CAGCTCGTGT TGTGAACATG ACTGTACGAA CTATCCTGGA 
GGATACGAGT GTTACTGTAC AGCCGGATAT CGACTGAACA CTGACGGATG CAGCTGTGAT 
GATATAGATG AGTGTTTGGC TGTTAATGGA GGATGTGATC ATGCTTGTCT GAACAGCGCC 
GGCTCTTTCC AGTGTTCCTG TAGACGTGGA TATCGACTCG ATGAGGACAG AAGATCCTGC 
ATACTGATGG AGGAGTCAGT GGAGGCGTTG AGCAGCGGTC AGACAGTCAA TAAACCTGGA 
CCAGGACCTC AGCTCACCAT CCTGCAGGAC TTCGATCAGA TCCTGGAGAA ATACGAGGAC 
TACGAGGACG ACACTGGAGA ACTGCGTGCC GAGAGCACTT TAGCTGAGAA ATTCATTTGT 
TTGAACGATT CGTTCGGCGT TGACTGCAGT CTGTCCTGTG AGGACTGTGT GAACGGAGGA 
GTGTGTAACA AACACAGAAA TGCCTGTGAC TGTCCTGACG GATGGACTGG AGTCGTCTGT 
AATGAGACAT GCCCTGAGGG TTGGTTTGGC AGGAATTGCT CACTGTTGTG TAAGTGTAAG 
AACGGTGGTG TGTGTGATCC GGTGTCCGGG AGCTGTCGCT GTCCACCAGG GGTCAGTGGA 
GAGCTGTGTC AGGACGGCTG TCCGAAGGGT CTCTATGGGA AGTTTTGCAA TAAAAGGTGT 
AACTGCGCTA ATAACGGACG TTGTCATCGC ACTTATGGAG CGTGTTTGTG TGACCCGGGA 
CTGTATGGAC GATTCTGCCA CCTTCCGTGT CCGAAGTGGA TGTTTGGTCC CGGTTGTTCA 
GAAGAGTGTC AGTGTGTCCA GCAGAACACT ATAGAGTGTC ACCGTCATCA TGGAGACTGC 
ATCTGCAAAC CTGGATACAG AGGCCACACC TGCAAAGACG AGTGTGAATC AGGGTTTTTT 
GGCTCTGCTT GTGAGAATAA ATGCTCTTGT CCGCCTGGAG TGACCTGTGA TCATGTGACT 
GGAGCTTGTC AACCCAAATG TCCAGCTGGA AAAACAGGCG ACAAATGTGA CCAAGATTGT 
GTGGAAGGGA GATTTGGGAT TGGATGCTCT CAGTCTTGTG ACTGCTCTGG CGCACCATGT 
GACAAAGTGA CTGGTCAATG CACGTGTCCT GCTGGAACGT TTGGAAAGCG CTGTGAAAAA 
GAGTGTCCTG AGGGCCGCTG GGGTCTGGGC TGTCAGGAAT GGTGTCCGGT GTGCGAGAAC 
GGAGGCCGCT GTGACCGGCG TAACGGCACA TGTGTGTGTT CTCCTGGATT CATCGGCAGA 
TTGTGTCAGA ACTCATGTCC TGCGGGTCGC TTCGGTGTGG GCTGTCAGCT CAGATGTGTG 
TGTGATCTCG GAGTTCGCTG TAATCCTGTA AGCGGACGCT GCTCGTGTCC TCCAGGCTGG 
ACGGGACACA ACTGCAGGAA GGTGTGTGAC GCGGGCCGCT GGGGTCTGGA CTGTGAGAGT 
GTATGTGACT GTCGTAATGC TGACGGTGTG TGTGATGCTC AGACTGGACG CTGTCATTGT 
GAGGCCGGAT TTACTGGAGC ACGATGTGAC CAGAAATGCC CGGCTGGCTC GTTTGGGCCC 
GGCTGTAAGC ATCGCTGCCA GTGTGAAAAT CAGGCATCAT GTGACCACGT GGGCGGAGCT 
TGTACCTGTA AGACCGGCTG GACCGGAACC TTCTGTGAAA AACCCTGTCC GCAGGGTTTC 
TATGGTCTGG ACTGTCAGCA GAAGTGTGTG TGTTTGAACG GCGGTCTCTG TGATCATGTG 
AGCGGTCAGT GTTTCTGTAA GGCCGGATGG ATCGGGACGC GCTGTAACCA GACGTGTCCT 
GCGGGATGGT TTGGCGTGAG CTGTTCTCAG ACCTGTGTTT GTCACAATAA CAGCAGCTGT 
GATGCAGTGA GCGGTCAGTG TGAATGCGGT GCAGGCTGGA CAGGAGACAC ATGCTCACAG 
CGCTGTCCTG CAGGTGTGTT CGGGGACGGT TGTGTTCACA GGTGTTTGTG TCAGAACGGG 
GCCTCGTGTG ACCCTGAGAG TGGACACTGC TTCTGTCCTA GCGGCTGGAC GGGGGCCGCC 
TGTCAGCTTG AATGTCCAGC GGGGAGGTTC GGCTCAGACT GTCAGGACAG ATGTGAATGT 
GTGAATGGAG CTCAGTGTGA TGGTCAGACG GGTCGCTGTG TTTGTCCGCC GGGCTGGACA 
GGAGAGCGCT GTGAGAATGA GTGTGAACGG GGCTGGTTTG GCGTGGGTTG TGAGGACCGC 
TGTCAGTGTG ATCACGGTGC TGTCTGCCAT CACGTGAGCG GTCAGTGTCT CTGCCCTGCT 
GGATGGAGAG GAAGACGCTG TGAGAAAGCA TGTCTGCCGG GCTCATTTGG TCAGGATTGT 
GTCCAGCGCT GCTCTTGTCC GTCCGGCTCC TCGTGTCATC ATGTGACCGG TCACTGTGGT 
TGTGCTCCAG GATTCACCGG CGACGGCTGT GAACACAGTT GTCTGCCTGG CACGTTCGGC 
CAGGACTGTA ATCAGGTGTG TCAGTGTTCA GAAAGAAACC AGCTCTGCCA CCCGGTGACT 
GGAGTGTGTT ACTGCGCGCC CGGATTCACT GGACTCAAGT GTGAACAGAC GTGTCCGCAG 
GGTTTGTACG GCTCAGGCTG TGAGCATCAG TGTGAGTGTC TGAATGGTGG AGTTTGTCTC 
TCAGACTCGG GATCCTGTCA GTGTCCAGCC GGATTCATTG GAGCTCAATG CAATCAAACA 
TGTCCAGCGG GTCGCTATGG TCTGGACTGT GCTCATGTGT CAGCGTGTGG GACAGGAGTG 
AGGAATGATC CGGCTACAGG AAGATGCACT GCACATCAAG TCTGTCCTCC AGGTTGGTTT 
GGTTCTGGCT GTTCTCAGCG TTGTGACTGC AGTAACAGAG GTGTGTGTGA CGGAGTCTCT 
GGAAACTGCT CATGTGCTCT CGGCTGGACC GGCAAACACT GTGATAAAGA GTGTCCTGAT 
GGCAGCTTTG GGCCTGACTG CTCTCTTCAG TGTTCCTGTC AGAATAACAG CACATGTGAC 
CGAGTGACAG GAAGTTGTCA TTGTCGTCCA GGATATTACG GTCATCTGTG TGAACACGCG 
TGTCCGGCTG GTCTCTTCGG CTCTGGGTGT CAGCAGCAGT GTGACTGTAT GAACGGAGCC 
TCCTGTCATC CGGATACAGG ACAGTGTGTG TGTCCCGCTG GAGTCCACGG CCCGCGCTGT 
CAGCACGTGT GTGCTCAGGG CAGGTTTGGT GTGAACTGCT CTCAGTCATG TGACTGTGCT 
GATGGAGCGT CATGTGACCC GGTCAGCGGC CGCTGTGTTT GTCCATCAGG GAAGACTGGA 
GTGCGGTGTG ACAGCGACTG CAGTGTGAAC CGGTTTGGCC CTGACTGCTC TCTGCTGTGC 
GAGTGTGAAA ATGGAGCTCA GTGCGACAGC AGAAATGGAC GCTGCTTCTG TCTGAGCGGC 
TGGATCGGCT TCACATGTTC ACAGAGCGCT CCTCCAGAAC TGAACTCCAG ACGGTCAGAA 
GAGGATCAGC TTCAGCTCAA CACTTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304569.1
CDS	508..5277
Translation	MKNVDLLLSLIILVDGINAVSRLGKDLKPHMPNVCAEKEVKLVAQMQPCVQAFTRMVKAWKQGCQGQSWCVGYERRTAYYTAYRQVYRQEQQTVYKCCPGWSQLNGEAGCLYPVCRSGVCFNGGQCKEGSAQLCVCPAGFSGPSCQYDVNECEETNGGCEALCCNTIGSFYCKCPAGQELKEDGKTCQDIDECQVHNGGCQHTCVNTRGSYHCQCNAGSRLHVDGRTCIAVQSCGVGNGGCEHFCVQQTAGHVQCRCRPDYRLDADGKHCTLVNPCAEQNGGCSQMCHRDGAQARCECHPGYQLAEDGKTCEDIDECALGQTDCAHGCRNTRGSFMCVCSAAYELGVDGKQCYRIEMEIVNSCDHDNGGCSHHCEHSTAGPLCSCNEGYQLDHDHKTCVDINECVDGSSCCEHDCTNYPGGYECYCTAGYRLNTDGCSCDDIDECLAVNGGCDHACLNSAGSFQCSCRRGYRLDEDRRSCILMEESVEALSSGQTVNKPGPGPQLTILQDFDQILEKYEDYEDDTGELRAESTLAEKFICLNDSFGVDCSLSCEDCVNGGVCNKHRNACDCPDGWTGVVCNETCPEGWFGRNCSLLCKCKNGGVCDPVSGSCRCPPGVSGELCQDGCPKGLYGKFCNKRCNCANNGRCHRTYGACLCDPGLYGRFCHLPCPKWMFGPGCSEECQCVQQNTIECHRHHGDCICKPGYRGHTCKDECESGFFGSACENKCSCPPGVTCDHVTGACQPKCPAGKTGDKCDQDCVEGRFGIGCSQSCDCSGAPCDKVTGQCTCPAGTFGKRCEKECPEGRWGLGCQEWCPVCENGGRCDRRNGTCVCSPGFIGRLCQNSCPAGRFGVGCQLRCVCDLGVRCNPVSGRCSCPPGWTGHNCRKVCDAGRWGLDCESVCDCRNADGVCDAQTGRCHCEAGFTGARCDQKCPAGSFGPGCKHRCQCENQASCDHVGGACTCKTGWTGTFCEKPCPQGFYGLDCQQKCVCLNGGLCDHVSGQCFCKAGWIGTRCNQTCPAGWFGVSCSQTCVCHNNSSCDAVSGQCECGAGWTGDTCSQRCPAGVFGDGCVHRCLCQNGASCDPESGHCFCPSGWTGAACQLECPAGRFGSDCQDRCECVNGAQCDGQTGRCVCPPGWTGERCENECERGWFGVGCEDRCQCDHGAVCHHVSGQCLCPAGWRGRRCEKACLPGSFGQDCVQRCSCPSGSSCHHVTGHCGCAPGFTGDGCEHSCLPGTFGQDCNQVCQCSERNQLCHPVTGVCYCAPGFTGLKCEQTCPQGLYGSGCEHQCECLNGGVCLSDSGSCQCPAGFIGAQCNQTCPAGRYGLDCAHVSACGTGVRNDPATGRCTAHQVCPPGWFGSGCSQRCDCSNRGVCDGVSGNCSCALGWTGKHCDKECPDGSFGPDCSLQCSCQNNSTCDRVTGSCHCRPGYYGHLCEHACPAGLFGSGCQQQCDCMNGASCHPDTGQCVCPAGVHGPRCQHVCAQGRFGVNCSQSCDCADGASCDPVSGRCVCPSGKTGVRCDSDCSVNRFGPDCSLLCECENGAQCDSRNGRCFCLSGWIGFTCSQSAPPELNSRRSEEDQLQLNTS

Target ORF information:

RefSeq Version	XM_009306294.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio multiple EGF-like-domains 6b (megf6b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306294.2

ORF Insert Sequence:

ATGAAGAACG TGGATTTATT ACTTTCCTTA ATCATTCTAG TTGATGGAAT TAATGCAGTA 
TCGCGTCTCG GGAAGGACCT GAAGCCGCAC ATGCCGAATG TGTGTGCAGA GAAGGAGGTG 
AAGCTGGTGG CTCAGATGCA GCCGTGTGTT CAAGCCTTCA CACGAATGGT TAAAGCATGG 
AAACAAGGTT GTCAGGGTCA GTCCTGGTGT GTGGGATACG AGAGGAGAAC AGCTTATTAT 
ACTGCATACA GACAGGTGTA TCGACAGGAA CAGCAGACGG TTTACAAGTG TTGTCCAGGA 
TGGTCACAGC TCAACGGAGA GGCCGGATGC CTGTACCCGG TCTGCAGGTC TGGTGTGTGT 
TTTAATGGCG GTCAGTGTAA AGAAGGTTCC GCTCAGCTCT GTGTGTGTCC AGCAGGATTC 
AGCGGCCCCA GCTGCCAATA TGATGTGAAC GAGTGTGAAG AGACCAATGG AGGCTGTGAG 
GCTTTATGCT GTAACACCAT CGGCAGCTTC TACTGCAAGT GTCCCGCAGG TCAAGAGCTC 
AAGGAGGATG GCAAGACCTG CCAAGATATT GACGAGTGCC AGGTTCATAA CGGCGGCTGC 
CAGCACACAT GTGTGAACAC TAGGGGTTCG TATCACTGCC AGTGTAACGC CGGCTCTCGA 
CTGCATGTGG ACGGACGCAC CTGCATCGCT GTTCAGTCCT GCGGCGTTGG GAATGGCGGC 
TGTGAGCATT TCTGTGTCCA GCAGACGGCG GGTCATGTCC AGTGTCGCTG CAGACCTGAT 
TACAGACTGG ACGCGGATGG AAAACACTGC ACATTGGTGA ACCCGTGTGC GGAGCAGAAT 
GGCGGATGTT CTCAGATGTG TCATAGGGAT GGAGCTCAGG CCCGCTGTGA ATGTCATCCA 
GGTTATCAGC TCGCTGAAGA CGGCAAGACA TGTGAAGACA TTGATGAGTG TGCGTTGGGT 
CAGACGGACT GTGCTCATGG CTGTCGTAAC ACCAGAGGAT CCTTCATGTG TGTGTGTTCT 
GCTGCCTATG AGCTCGGGGT GGACGGTAAA CAGTGCTATC GTATTGAGAT GGAGATCGTG 
AACAGCTGTG ATCATGATAA TGGTGGTTGT TCTCATCACT GTGAACATTC GACCGCTGGT 
CCGCTGTGCA GCTGTAATGA AGGATATCAG CTGGACCACG ACCACAAGAC CTGCGTCGAT 
ATAAACGAGT GTGTCGACGG CAGCTCGTGT TGTGAACATG ACTGTACGAA CTATCCTGGA 
GGATACGAGT GTTACTGTAC AGCCGGATAT CGACTGAACA CTGACGGATG CAGCTGTGAT 
GATATAGATG AGTGTTTGGC TGTTAATGGA GGATGTGATC ATGCTTGTCT GAACAGCGCC 
GGCTCTTTCC AGTGTTCCTG TAGACGTGGA TATCGACTCG ATGAGGACAG AAGATCCTGC 
ATACTGATGG AGGAGTCAGT GGAGGCGTTG AGCAGCGGTC AGACAGTCAA TAAACCTGGA 
CCAGGACCTC AGCTCACCAT CCTGCAGGAC TTCGATCAGA TCCTGGAGAA ATACGAGGAC 
TACGAGGACG ACACTGGAGA ACTGCGTGCC GAGAGCACTT TAGCTGAGAA ATTCATTTGT 
TTGAACGATT CGTTCGGCGT TGACTGCAGT CTGTCCTGTG AGGACTGTGT GAACGGAGGA 
GTGTGTAACA AACACAGAAA TGCCTGTGAC TGTCCTGACG GATGGACTGG AGTCGTCTGT 
AATGAGACAT GCCCTGAGGG TTGGTTTGGC AGGAATTGCT CACTGTTGTG TAAGTGTAAG 
AACGGTGGTG TGTGTGATCC GGTGTCCGGG AGCTGTCGCT GTCCACCAGG GGTCAGTGGA 
GAGCTGTGTC AGGACGGCTG TCCGAAGGGT CTCTATGGGA AGTTTTGCAA TAAAAGGTGT 
AACTGCGCTA ATAACGGACG TTGTCATCGC ACTTATGGAG CGTGTTTGTG TGACCCGGGA 
CTGTATGGAC GATTCTGCCA CCTTCCGTGT CCGAAGTGGA TGTTTGGTCC CGGTTGTTCA 
GAAGAGTGTC AGTGTGTCCA GCAGAACACT ATAGAGTGTC ACCGTCATCA TGGAGACTGC 
ATCTGCAAAC CTGGATACAG AGGCCACACC TGCAAAGACG AGTGTGAATC AGGGTTTTTT 
GGCTCTGCTT GTGAGAATAA ATGCTCTTGT CCGCCTGGAG TGACCTGTGA TCATGTGACT 
GGAGCTTGTC AACCCAAATG TCCAGCTGGA AAAACAGGCG ACAAATGTGA CCAAGATTGT 
GTGGAAGGGA GATTTGGGAT TGGATGCTCT CAGTCTTGTG ACTGCTCTGG CGCACCATGT 
GACAAAGTGA CTGGTCAATG CACGTGTCCT GCTGGAACGT TTGGAAAGCG CTGTGAAAAA 
GAGTGTCCTG AGGGCCGCTG GGGTCTGGGC TGTCAGGAAT GGTGTCCGGT GTGCGAGAAC 
GGAGGCCGCT GTGACCGGCG TAACGGCACA TGTGTGTGTT CTCCTGGATT CATCGGCAGA 
TTGTGTCAGA ACTCATGTCC TGCGGGTCGC TTCGGTGTGG GCTGTCAGCT CAGATGTGTG 
TGTGATCTCG GAGTTCGCTG TAATCCTGTA AGCGGACGCT GCTCGTGTCC TCCAGGCTGG 
ACGGGACACA ACTGCAGGAA GGTGTGTGAC GCGGGCCGCT GGGGTCTGGA CTGTGAGAGT 
GTATGTGACT GTCGTAATGC TGACGGTGTG TGTGATGCTC AGACTGGACG CTGTCATTGT 
GAGGCCGGAT TTACTGGAGC ACGATGTGAC CAGAAATGCC CGGCTGGCTC GTTTGGGCCC 
GGCTGTAAGC ATCGCTGCCA GTGTGAAAAT CAGGCATCAT GTGACCACGT GGGCGGAGCT 
TGTACCTGTA AGACCGGCTG GACCGGAACC TTCTGTGAAA AACCCTGTCC GCAGGGTTTC 
TATGGTCTGG ACTGTCAGCA GAAGTGTGTG TGTTTGAACG GCGGTCTCTG TGATCATGTG 
AGCGGTCAGT GTTTCTGTAA GGCCGGATGG ATCGGGACGC GCTGTAACCA GACGTGTCCT 
GCGGGATGGT TTGGCGTGAG CTGTTCTCAG ACCTGTGTTT GTCACAATAA CAGCAGCTGT 
GATGCAGTGA GCGGTCAGTG TGAATGCGGT GCAGGCTGGA CAGGAGACAC ATGCTCACAG 
CGCTGTCCTG CAGGTGTGTT CGGGGACGGT TGTGTTCACA GGTGTTTGTG TCAGAACGGG 
GCCTCGTGTG ACCCTGAGAG TGGACACTGC TTCTGTCCTA GCGGCTGGAC GGGGGCCGCC 
TGTCAGCTTG AATGTCCAGC GGGGAGGTTC GGCTCAGACT GTCAGGACAG ATGTGAATGT 
GTGAATGGAG CTCAGTGTGA TGGTCAGACG GGTCGCTGTG TTTGTCCGCC GGGCTGGACA 
GGAGAGCGCT GTGAGAATGA GTGTGAACGG GGCTGGTTTG GCGTGGGTTG TGAGGACCGC 
TGTCAGTGTG ATCACGGTGC TGTCTGCCAT CACGTGAGCG GTCAGTGTCT CTGCCCTGCT 
GGATGGAGAG GAAGACGCTG TGAGAAAGCA TGTCTGCCGG GCTCATTTGG TCAGGATTGT 
GTCCAGCGCT GCTCTTGTCC GTCCGGCTCC TCGTGTCATC ATGTGACCGG TCACTGTGGT 
TGTGCTCCAG GATTCACCGG CGACGGCTGT GAACACAGTT GTCTGCCTGG CACGTTCGGC 
CAGGACTGTA ATCAGGTGTG TCAGTGTTCA GAAAGAAACC AGCTCTGCCA CCCGGTGACT 
GGAGTGTGTT ACTGCGCGCC CGGATTCACT GGACTCAAGT GTGAACAGAC GTGTCCGCAG 
GGTTTGTACG GCTCAGGCTG TGAGCATCAG TGTGAGTGTC TGAATGGTGG AGTTTGTCTC 
TCAGACTCGG GATCCTGTCA GTGTCCAGCC GGATTCATTG GAGCTCAATG CAATCAAACA 
TGTCCAGCGG GTCGCTATGG TCTGGACTGT GCTCATGTGT CAGCGTGTGG GACAGGAGTG 
AGGAATGATC CGGCTACAGG AAGATGCACT GCACATCAAG TCTGTCCTCC AGGTTGGTTT 
GGTTCTGGCT GTTCTCAGCG TTGTGACTGC AGTAACAGAG GTGTGTGTGA CGGAGTCTCT 
GGAAACTGCT CATGTGCTCT CGGCTGGACC GGCAAACACT GTGATAAAGA GTGTCCTGAT 
GGCAGCTTTG GGCCTGACTG CTCTCTTCAG TGTTCCTGTC AGAATAACAG CACATGTGAC 
CGAGTGACAG GAAGTTGTCA TTGTCGTCCA GGATATTACG GTCATCTGTG TGAACACGCG 
TGTCCGGCTG GTCTCTTCGG CTCTGGGTGT CAGCAGCAGT GTGACTGTAT GAACGGAGCC 
TCCTGTCATC CGGATACAGG ACAGTGTGTG TGTCCCGCTG GAGTCCACGG CCCGCGCTGT 
CAGCACGTGT GTGCTCAGGG CAGGTTTGGT GTGAACTGCT CTCAGTCATG TGACTGTGCT 
GATGGAGCGT CATGTGACCC GGTCAGCGGC CGCTGTGTTT GTCCATCAGG GAAGACTGGA 
GTGCGGTGTG ACAGCGACTG CAGTGTGAAC CGGTTTGGCC CTGACTGCTC TCTGCTGTGC 
GAGTGTGAAA ATGGAGCTCA GTGCGACAGC AGAAATGGAC GCTGCTTCTG TCTGAGCGGC 
TGGATCGGCT TCACATGTTC ACAGAGCGCT CCTCCAGAAC TGAACTCCAG ACGGTCAGAA 
GAGGATCAGC TTCAGCTCAA CACTTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF6B ( XM_009306297.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304572.1 Danio rerio multiple EGF-like-domains 6b (megf6b), transcript variant X2, mRNA.

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CloneID	ODa42667
Clone ID Related Accession (Same CDS sequence)	XM_009306297.2
Accession Version	XM_009306297.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	multiple epidermal growth factor-like domains protein 6 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306297.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAT GGCTCGGGCT CTGTCTTCTG TGCGTCGGGG TTAATATTCG TCCCGTTGAT 
GTTGCCTATT ATTCTGGCTA TTGGCATCAT TTGAGGCTCT GTCACAGGCC ACAGTGTTTT 
CATGGATACA GCAGATTTAA TATGGGATAC TGGATACCAG ATATTGACGA GTGCCAGGTT 
CATAACGGCG GCTGCCAGCA CACATGTGTG AACACTAGGG GTTCGTATCA CTGCCAGTGT 
AACGCCGGCT CTCGACTGCA TGTGGACGGA CGCACCTGCA TCGCTGTTCA GTCCTGCGGC 
GTTGGGAATG GCGGCTGTGA GCATTTCTGT GTCCAGCAGA CGGCGGGTCA TGTCCAGTGT 
CGCTGCAGAC CTGATTACAG ACTGGACGCG GATGGAAAAC ACTGCACATT GGTGAACCCG 
TGTGCGGAGC AGAATGGCGG ATGTTCTCAG ATGTGTCATA GGGATGGAGC TCAGGCCCGC 
TGTGAATGTC ATCCAGGTTA TCAGCTCGCT GAAGACGGCA AGACATGTGA AGACATTGAT 
GAGTGTGCGT TGGGTCAGAC GGACTGTGCT CATGGCTGTC GTAACACCAG AGGATCCTTC 
ATGTGTGTGT GTTCTGCTGC CTATGAGCTC GGGGTGGACG GTAAACAGTG CTATCGTATT 
GAGATGGAGA TCGTGAACAG CTGTGATCAT GATAATGGTG GTTGTTCTCA TCACTGTGAA 
CATTCGACCG CTGGTCCGCT GTGCAGCTGT AATGAAGGAT ATCAGCTGGA CCACGACCAC 
AAGACCTGCG TCGATATAAA CGAGTGTGTC GACGGCAGCT CGTGTTGTGA ACATGACTGT 
ACGAACTATC CTGGAGGATA CGAGTGTTAC TGTACAGCCG GATATCGACT GAACACTGAC 
GGATGCAGCT GTGATGATAT AGATGAGTGT TTGGCTGTTA ATGGAGGATG TGATCATGCT 
TGTCTGAACA GCGCCGGCTC TTTCCAGTGT TCCTGTAGAC GTGGATATCG ACTCGATGAG 
GACAGAAGAT CCTGCATACT GATGGAGGAG TCAGTGGAGG CGTTGAGCAG CGGTCAGACA 
GTCAATAAAC CTGGACCAGG ACCTCAGCTC ACCATCCTGC AGGACTTCGA TCAGATCCTG 
GAGAAATACG AGGACTACGA GGACGACACT GGAGAACTGC GTGCCGAGAG CACTTTAGCT 
GAGAAATTCA TTTGTTTGAA CGATTCGTTC GGCGTTGACT GCAGTCTGTC CTGTGAGGAC 
TGTGTGAACG GAGGAGTGTG TAACAAACAC AGAAATGCCT GTGACTGTCC TGACGGATGG 
ACTGGAGTCG TCTGTAATGA GACATGCCCT GAGGGTTGGT TTGGCAGGAA TTGCTCACTG 
TTGTGTAAGT GTAAGAACGG TGGTGTGTGT GATCCGGTGT CCGGGAGCTG TCGCTGTCCA 
CCAGGGGTCA GTGGAGAGCT GTGTCAGGAC GGCTGTCCGA AGGGTCTCTA TGGGAAGTTT 
TGCAATAAAA GGTGTAACTG CGCTAATAAC GGACGTTGTC ATCGCACTTA TGGAGCGTGT 
TTGTGTGACC CGGGACTGTA TGGACGATTC TGCCACCTTC CGTGTCCGAA GTGGATGTTT 
GGTCCCGGTT GTTCAGAAGA GTGTCAGTGT GTCCAGCAGA ACACTATAGA GTGTCACCGT 
CATCATGGAG ACTGCATCTG CAAACCTGGA TACAGAGGCC ACACCTGCAA AGACGAGTGT 
GAATCAGGGT TTTTTGGCTC TGCTTGTGAG AATAAATGCT CTTGTCCGCC TGGAGTGACC 
TGTGATCATG TGACTGGAGC TTGTCAACCC AAATGTCCAG CTGGAAAAAC AGGCGACAAA 
TGTGACCAAG ATTGTGTGGA AGGGAGATTT GGGATTGGAT GCTCTCAGTC TTGTGACTGC 
TCTGGCGCAC CATGTGACAA AGTGACTGGT CAATGCACGT GTCCTGCTGG AACGTTTGGA 
AAGCGCTGTG AAAAAGAGTG TCCTGAGGGC CGCTGGGGTC TGGGCTGTCA GGAATGGTGT 
CCGGTGTGCG AGAACGGAGG CCGCTGTGAC CGGCGTAACG GCACATGTGT GTGTTCTCCT 
GGATTCATCG GCAGATTGTG TCAGAACTCA TGTCCTGCGG GTCGCTTCGG TGTGGGCTGT 
CAGCTCAGAT GTGTGTGTGA TCTCGGAGTT CGCTGTAATC CTGTAAGCGG ACGCTGCTCG 
TGTCCTCCAG GCTGGACGGG ACACAACTGC AGGAAGGTGT GTGACGCGGG CCGCTGGGGT 
CTGGACTGTG AGAGTGTATG TGACTGTCGT AATGCTGACG GTGTGTGTGA TGCTCAGACT 
GGACGCTGTC ATTGTGAGGC CGGATTTACT GGAGCACGAT GTGACCAGAA ATGCCCGGCT 
GGCTCGTTTG GGCCCGGCTG TAAGCATCGC TGCCAGTGTG AAAATCAGGC ATCATGTGAC 
CACGTGGGCG GAGCTTGTAC CTGTAAGACC GGCTGGACCG GAACCTTCTG TGAAAAACCC 
TGTCCGCAGG GTTTCTATGG TCTGGACTGT CAGCAGAAGT GTGTGTGTTT GAACGGCGGT 
CTCTGTGATC ATGTGAGCGG TCAGTGTTTC TGTAAGGCCG GATGGATCGG GACGCGCTGT 
AACCAGACGT GTCCTGCGGG ATGGTTTGGC GTGAGCTGTT CTCAGACCTG TGTTTGTCAC 
AATAACAGCA GCTGTGATGC AGTGAGCGGT CAGTGTGAAT GCGGTGCAGG CTGGACAGGA 
GACACATGCT CACAGCGCTG TCCTGCAGGT GTGTTCGGGG ACGGTTGTGT TCACAGGTGT 
TTGTGTCAGA ACGGGGCCTC GTGTGACCCT GAGAGTGGAC ACTGCTTCTG TCCTAGCGGC 
TGGACGGGGG CCGCCTGTCA GCTTGAATGT CCAGCGGGGA GGTTCGGCTC AGACTGTCAG 
GACAGATGTG AATGTGTGAA TGGAGCTCAG TGTGATGGTC AGACGGGTCG CTGTGTTTGT 
CCGCCGGGCT GGACAGGAGA GCGCTGTGAG AATGAGTGTG AACGGGGCTG GTTTGGCGTG 
GGTTGTGAGG ACCGCTGTCA GTGTGATCAC GGTGCTGTCT GCCATCACGT GAGCGGTCAG 
TGTCTCTGCC CTGCTGGATG GAGAGGAAGA CGCTGTGAGA AAGCATGTCT GCCGGGCTCA 
TTTGGTCAGG ATTGTGTCCA GCGCTGCTCT TGTCCGTCCG GCTCCTCGTG TCATCATGTG 
ACCGGTCACT GTGGTTGTGC TCCAGGATTC ACCGGCGACG GCTGTGAACA CAGTTGTCTG 
CCTGGCACGT TCGGCCAGGA CTGTAATCAG GTGTGTCAGT GTTCAGAAAG AAACCAGCTC 
TGCCACCCGG TGACTGGAGT GTGTTACTGC GCGCCCGGAT TCACTGGACT CAAGTGTGAA 
CAGACGTGTC CGCAGGGTTT GTACGGCTCA GGCTGTGAGC ATCAGTGTGA GTGTCTGAAT 
GGTGGAGTTT GTCTCTCAGA CTCGGGATCC TGTCAGTGTC CAGCCGGATT CATTGGAGCT 
CAATGCAATC AAACATGTCC AGCGGGTCGC TATGGTCTGG ACTGTGCTCA TGTGTCAGCG 
TGTGGGACAG GAGTGAGGAA TGATCCGGCT ACAGGAAGAT GCACTGCACA TCAAGTCTGT 
CCTCCAGGTT GGTTTGGTTC TGGCTGTTCT CAGCGTTGTG ACTGCAGTAA CAGAGGTGTG 
TGTGACGGAG TCTCTGGAAA CTGCTCATGT GCTCTCGGCT GGACCGGCAA ACACTGTGAT 
AAAGAGTGTC CTGATGGCAG CTTTGGGCCT GACTGCTCTC TTCAGTGTTC CTGTCAGAAT 
AACAGCACAT GTGACCGAGT GACAGGAAGT TGTCATTGTC GTCCAGGATA TTACGGTCAT 
CTGTGTGAAC ACGCGTGTCC GGCTGGTCTC TTCGGCTCTG GGTGTCAGCA GCAGTGTGAC 
TGTATGAACG GAGCCTCCTG TCATCCGGAT ACAGGACAGT GTGTGTGTCC CGCTGGAGTC 
CACGGCCCGC GCTGTCAGCA CGTGTGTGCT CAGGGCAGGT TTGGTGTGAA CTGCTCTCAG 
TCATGTGACT GTGCTGATGG AGCGTCATGT GACCCGGTCA GCGGCCGCTG TGTTTGTCCA 
TCAGGGAAGA CTGGAGTGCG GTGTGACAGC GACTGCAGTG TGAACCGGTT TGGCCCTGAC 
TGCTCTCTGC TGTGCGAGTG TGAAAATGGA GCTCAGTGCG ACAGCAGAAA TGGACGCTGC 
TTCTGTCTGA GCGGCTGGAT CGGCTTCACA TGTTCACAGA GCGCTCCTCC AGAACTGAAC 
TCCAGACGGT CAGAAGAGGA TCAGCTTCAG CTCAACACTT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304572.1
CDS	49..4413
Translation	MSGWLGLCLLCVGVNIRPVDVAYYSGYWHHLRLCHRPQCFHGYSRFNMGYWIPDIDECQVHNGGCQHTCVNTRGSYHCQCNAGSRLHVDGRTCIAVQSCGVGNGGCEHFCVQQTAGHVQCRCRPDYRLDADGKHCTLVNPCAEQNGGCSQMCHRDGAQARCECHPGYQLAEDGKTCEDIDECALGQTDCAHGCRNTRGSFMCVCSAAYELGVDGKQCYRIEMEIVNSCDHDNGGCSHHCEHSTAGPLCSCNEGYQLDHDHKTCVDINECVDGSSCCEHDCTNYPGGYECYCTAGYRLNTDGCSCDDIDECLAVNGGCDHACLNSAGSFQCSCRRGYRLDEDRRSCILMEESVEALSSGQTVNKPGPGPQLTILQDFDQILEKYEDYEDDTGELRAESTLAEKFICLNDSFGVDCSLSCEDCVNGGVCNKHRNACDCPDGWTGVVCNETCPEGWFGRNCSLLCKCKNGGVCDPVSGSCRCPPGVSGELCQDGCPKGLYGKFCNKRCNCANNGRCHRTYGACLCDPGLYGRFCHLPCPKWMFGPGCSEECQCVQQNTIECHRHHGDCICKPGYRGHTCKDECESGFFGSACENKCSCPPGVTCDHVTGACQPKCPAGKTGDKCDQDCVEGRFGIGCSQSCDCSGAPCDKVTGQCTCPAGTFGKRCEKECPEGRWGLGCQEWCPVCENGGRCDRRNGTCVCSPGFIGRLCQNSCPAGRFGVGCQLRCVCDLGVRCNPVSGRCSCPPGWTGHNCRKVCDAGRWGLDCESVCDCRNADGVCDAQTGRCHCEAGFTGARCDQKCPAGSFGPGCKHRCQCENQASCDHVGGACTCKTGWTGTFCEKPCPQGFYGLDCQQKCVCLNGGLCDHVSGQCFCKAGWIGTRCNQTCPAGWFGVSCSQTCVCHNNSSCDAVSGQCECGAGWTGDTCSQRCPAGVFGDGCVHRCLCQNGASCDPESGHCFCPSGWTGAACQLECPAGRFGSDCQDRCECVNGAQCDGQTGRCVCPPGWTGERCENECERGWFGVGCEDRCQCDHGAVCHHVSGQCLCPAGWRGRRCEKACLPGSFGQDCVQRCSCPSGSSCHHVTGHCGCAPGFTGDGCEHSCLPGTFGQDCNQVCQCSERNQLCHPVTGVCYCAPGFTGLKCEQTCPQGLYGSGCEHQCECLNGGVCLSDSGSCQCPAGFIGAQCNQTCPAGRYGLDCAHVSACGTGVRNDPATGRCTAHQVCPPGWFGSGCSQRCDCSNRGVCDGVSGNCSCALGWTGKHCDKECPDGSFGPDCSLQCSCQNNSTCDRVTGSCHCRPGYYGHLCEHACPAGLFGSGCQQQCDCMNGASCHPDTGQCVCPAGVHGPRCQHVCAQGRFGVNCSQSCDCADGASCDPVSGRCVCPSGKTGVRCDSDCSVNRFGPDCSLLCECENGAQCDSRNGRCFCLSGWIGFTCSQSAPPELNSRRSEEDQLQLNTS

Target ORF information:

RefSeq Version	XM_009306297.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio multiple EGF-like-domains 6b (megf6b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306297.2

ORF Insert Sequence:

ATGTCAGGAT GGCTCGGGCT CTGTCTTCTG TGCGTCGGGG TTAATATTCG TCCCGTTGAT 
GTTGCCTATT ATTCTGGCTA TTGGCATCAT TTGAGGCTCT GTCACAGGCC ACAGTGTTTT 
CATGGATACA GCAGATTTAA TATGGGATAC TGGATACCAG ATATTGACGA GTGCCAGGTT 
CATAACGGCG GCTGCCAGCA CACATGTGTG AACACTAGGG GTTCGTATCA CTGCCAGTGT 
AACGCCGGCT CTCGACTGCA TGTGGACGGA CGCACCTGCA TCGCTGTTCA GTCCTGCGGC 
GTTGGGAATG GCGGCTGTGA GCATTTCTGT GTCCAGCAGA CGGCGGGTCA TGTCCAGTGT 
CGCTGCAGAC CTGATTACAG ACTGGACGCG GATGGAAAAC ACTGCACATT GGTGAACCCG 
TGTGCGGAGC AGAATGGCGG ATGTTCTCAG ATGTGTCATA GGGATGGAGC TCAGGCCCGC 
TGTGAATGTC ATCCAGGTTA TCAGCTCGCT GAAGACGGCA AGACATGTGA AGACATTGAT 
GAGTGTGCGT TGGGTCAGAC GGACTGTGCT CATGGCTGTC GTAACACCAG AGGATCCTTC 
ATGTGTGTGT GTTCTGCTGC CTATGAGCTC GGGGTGGACG GTAAACAGTG CTATCGTATT 
GAGATGGAGA TCGTGAACAG CTGTGATCAT GATAATGGTG GTTGTTCTCA TCACTGTGAA 
CATTCGACCG CTGGTCCGCT GTGCAGCTGT AATGAAGGAT ATCAGCTGGA CCACGACCAC 
AAGACCTGCG TCGATATAAA CGAGTGTGTC GACGGCAGCT CGTGTTGTGA ACATGACTGT 
ACGAACTATC CTGGAGGATA CGAGTGTTAC TGTACAGCCG GATATCGACT GAACACTGAC 
GGATGCAGCT GTGATGATAT AGATGAGTGT TTGGCTGTTA ATGGAGGATG TGATCATGCT 
TGTCTGAACA GCGCCGGCTC TTTCCAGTGT TCCTGTAGAC GTGGATATCG ACTCGATGAG 
GACAGAAGAT CCTGCATACT GATGGAGGAG TCAGTGGAGG CGTTGAGCAG CGGTCAGACA 
GTCAATAAAC CTGGACCAGG ACCTCAGCTC ACCATCCTGC AGGACTTCGA TCAGATCCTG 
GAGAAATACG AGGACTACGA GGACGACACT GGAGAACTGC GTGCCGAGAG CACTTTAGCT 
GAGAAATTCA TTTGTTTGAA CGATTCGTTC GGCGTTGACT GCAGTCTGTC CTGTGAGGAC 
TGTGTGAACG GAGGAGTGTG TAACAAACAC AGAAATGCCT GTGACTGTCC TGACGGATGG 
ACTGGAGTCG TCTGTAATGA GACATGCCCT GAGGGTTGGT TTGGCAGGAA TTGCTCACTG 
TTGTGTAAGT GTAAGAACGG TGGTGTGTGT GATCCGGTGT CCGGGAGCTG TCGCTGTCCA 
CCAGGGGTCA GTGGAGAGCT GTGTCAGGAC GGCTGTCCGA AGGGTCTCTA TGGGAAGTTT 
TGCAATAAAA GGTGTAACTG CGCTAATAAC GGACGTTGTC ATCGCACTTA TGGAGCGTGT 
TTGTGTGACC CGGGACTGTA TGGACGATTC TGCCACCTTC CGTGTCCGAA GTGGATGTTT 
GGTCCCGGTT GTTCAGAAGA GTGTCAGTGT GTCCAGCAGA ACACTATAGA GTGTCACCGT 
CATCATGGAG ACTGCATCTG CAAACCTGGA TACAGAGGCC ACACCTGCAA AGACGAGTGT 
GAATCAGGGT TTTTTGGCTC TGCTTGTGAG AATAAATGCT CTTGTCCGCC TGGAGTGACC 
TGTGATCATG TGACTGGAGC TTGTCAACCC AAATGTCCAG CTGGAAAAAC AGGCGACAAA 
TGTGACCAAG ATTGTGTGGA AGGGAGATTT GGGATTGGAT GCTCTCAGTC TTGTGACTGC 
TCTGGCGCAC CATGTGACAA AGTGACTGGT CAATGCACGT GTCCTGCTGG AACGTTTGGA 
AAGCGCTGTG AAAAAGAGTG TCCTGAGGGC CGCTGGGGTC TGGGCTGTCA GGAATGGTGT 
CCGGTGTGCG AGAACGGAGG CCGCTGTGAC CGGCGTAACG GCACATGTGT GTGTTCTCCT 
GGATTCATCG GCAGATTGTG TCAGAACTCA TGTCCTGCGG GTCGCTTCGG TGTGGGCTGT 
CAGCTCAGAT GTGTGTGTGA TCTCGGAGTT CGCTGTAATC CTGTAAGCGG ACGCTGCTCG 
TGTCCTCCAG GCTGGACGGG ACACAACTGC AGGAAGGTGT GTGACGCGGG CCGCTGGGGT 
CTGGACTGTG AGAGTGTATG TGACTGTCGT AATGCTGACG GTGTGTGTGA TGCTCAGACT 
GGACGCTGTC ATTGTGAGGC CGGATTTACT GGAGCACGAT GTGACCAGAA ATGCCCGGCT 
GGCTCGTTTG GGCCCGGCTG TAAGCATCGC TGCCAGTGTG AAAATCAGGC ATCATGTGAC 
CACGTGGGCG GAGCTTGTAC CTGTAAGACC GGCTGGACCG GAACCTTCTG TGAAAAACCC 
TGTCCGCAGG GTTTCTATGG TCTGGACTGT CAGCAGAAGT GTGTGTGTTT GAACGGCGGT 
CTCTGTGATC ATGTGAGCGG TCAGTGTTTC TGTAAGGCCG GATGGATCGG GACGCGCTGT 
AACCAGACGT GTCCTGCGGG ATGGTTTGGC GTGAGCTGTT CTCAGACCTG TGTTTGTCAC 
AATAACAGCA GCTGTGATGC AGTGAGCGGT CAGTGTGAAT GCGGTGCAGG CTGGACAGGA 
GACACATGCT CACAGCGCTG TCCTGCAGGT GTGTTCGGGG ACGGTTGTGT TCACAGGTGT 
TTGTGTCAGA ACGGGGCCTC GTGTGACCCT GAGAGTGGAC ACTGCTTCTG TCCTAGCGGC 
TGGACGGGGG CCGCCTGTCA GCTTGAATGT CCAGCGGGGA GGTTCGGCTC AGACTGTCAG 
GACAGATGTG AATGTGTGAA TGGAGCTCAG TGTGATGGTC AGACGGGTCG CTGTGTTTGT 
CCGCCGGGCT GGACAGGAGA GCGCTGTGAG AATGAGTGTG AACGGGGCTG GTTTGGCGTG 
GGTTGTGAGG ACCGCTGTCA GTGTGATCAC GGTGCTGTCT GCCATCACGT GAGCGGTCAG 
TGTCTCTGCC CTGCTGGATG GAGAGGAAGA CGCTGTGAGA AAGCATGTCT GCCGGGCTCA 
TTTGGTCAGG ATTGTGTCCA GCGCTGCTCT TGTCCGTCCG GCTCCTCGTG TCATCATGTG 
ACCGGTCACT GTGGTTGTGC TCCAGGATTC ACCGGCGACG GCTGTGAACA CAGTTGTCTG 
CCTGGCACGT TCGGCCAGGA CTGTAATCAG GTGTGTCAGT GTTCAGAAAG AAACCAGCTC 
TGCCACCCGG TGACTGGAGT GTGTTACTGC GCGCCCGGAT TCACTGGACT CAAGTGTGAA 
CAGACGTGTC CGCAGGGTTT GTACGGCTCA GGCTGTGAGC ATCAGTGTGA GTGTCTGAAT 
GGTGGAGTTT GTCTCTCAGA CTCGGGATCC TGTCAGTGTC CAGCCGGATT CATTGGAGCT 
CAATGCAATC AAACATGTCC AGCGGGTCGC TATGGTCTGG ACTGTGCTCA TGTGTCAGCG 
TGTGGGACAG GAGTGAGGAA TGATCCGGCT ACAGGAAGAT GCACTGCACA TCAAGTCTGT 
CCTCCAGGTT GGTTTGGTTC TGGCTGTTCT CAGCGTTGTG ACTGCAGTAA CAGAGGTGTG 
TGTGACGGAG TCTCTGGAAA CTGCTCATGT GCTCTCGGCT GGACCGGCAA ACACTGTGAT 
AAAGAGTGTC CTGATGGCAG CTTTGGGCCT GACTGCTCTC TTCAGTGTTC CTGTCAGAAT 
AACAGCACAT GTGACCGAGT GACAGGAAGT TGTCATTGTC GTCCAGGATA TTACGGTCAT 
CTGTGTGAAC ACGCGTGTCC GGCTGGTCTC TTCGGCTCTG GGTGTCAGCA GCAGTGTGAC 
TGTATGAACG GAGCCTCCTG TCATCCGGAT ACAGGACAGT GTGTGTGTCC CGCTGGAGTC 
CACGGCCCGC GCTGTCAGCA CGTGTGTGCT CAGGGCAGGT TTGGTGTGAA CTGCTCTCAG 
TCATGTGACT GTGCTGATGG AGCGTCATGT GACCCGGTCA GCGGCCGCTG TGTTTGTCCA 
TCAGGGAAGA CTGGAGTGCG GTGTGACAGC GACTGCAGTG TGAACCGGTT TGGCCCTGAC 
TGCTCTCTGC TGTGCGAGTG TGAAAATGGA GCTCAGTGCG ACAGCAGAAA TGGACGCTGC 
TTCTGTCTGA GCGGCTGGAT CGGCTTCACA TGTTCACAGA GCGCTCCTCC AGAACTGAAC 
TCCAGACGGT CAGAAGAGGA TCAGCTTCAG CTCAACACTT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MEGF6B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

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