megf6a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	megf6a
Entrez Gene ID	565108
Full Name	multiple EGF-like-domains 6a
Synonyms	si:dkey-25b23.2
General protein information	Preferred Names multiple EGF-like-domains 6a Names multiple epidermal growth factor-like domains protein 6
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021473660.1	XP_021329335.1	multiple epidermal growth factor-like domains protein 6 isoform X2
XM_017353739.1	XP_017209228.1	multiple epidermal growth factor-like domains protein 6 isoform X1
XM_017353739.2	XP_017209228.2	multiple epidermal growth factor-like domains protein 6 isoform X1
XM_021473659.1	XP_021329334.1	multiple epidermal growth factor-like domains protein 6 isoform X1
XM_009296882.2	XP_009295157.1	multiple epidermal growth factor-like domains protein 6 isoform X2
XM_009296882.3	XP_009295157.1	multiple epidermal growth factor-like domains protein 6 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MEGF6	XP_546739.4
Mus musculus (house mouse)	Megf6	NP_001156449.1
Rattus norvegicus (Norway rat)	Megf6	NP_075244.1
Rattus norvegicus (Norway rat)	LOC100911486	XP_006239572.1
Danio rerio (zebrafish)	LOC557764	XP_685976.6
Caenorhabditis elegans (roundworm)	CELE_Y64G10A.7	NP_001255803.1
Pan troglodytes (chimpanzee)	MEGF6	XP_513736.3
Gallus gallus (chicken)	MEGF6	XP_004947431.1
Homo sapiens (human)	MEGF6	NP_001400.3
Bos taurus (cattle)	MEGF6	XP_005217247.1
Danio rerio (zebrafish)	megf6a	XP_693497.6
Xenopus tropicalis (tropical clawed frog)	megf6	XP_002933906.2

Gene Ontology

The following megf6a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the megf6a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa146261	XM_021473660.1 Latest version!	Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa57519	XM_017353739.1 Latest version!	Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa146260	XM_021473659.1 Latest version!	Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa151555	XM_017353739.2 Latest version!	Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa44516	XM_009296882.3 Latest version!	Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa44516	XM_009296882.2	Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

megf6a ( XM_021473660.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329335.1 Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa146261
Clone ID Related Accession (Same CDS sequence)	XM_021473660.1
Accession Version	XM_021473660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	multiple epidermal growth factor-like domains protein 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395144.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCCG ATTGGTTGAG GTTGGTGCTT GTGTTTACCT TCTGGACTCA GCTGGTACCA 
AGTTTGGCTG CTGGAAATCC ATATTACCGC TACTATCACA GAAACTGCTA CACCTGCTTC 
AATGGATATC ATGCGTACAA CACAGGACAC CAGGGCGCAG ATATTGATGA GTGCCAGGTT 
CACAACGGAG GCTGCCAGCA CAGATGTGTG AACACTAGAG GCTCGTACTA CTGCGAGTGC 
AATCCAGGCT TTCGGCTCCA CGTTGATGGC CGCACCTGCA TAGCTGTTTT ATCCTGTTCG 
GTAAATAATG GAGGTTGTGA GCATGAATGT GTCCAGCTTT CTCCAGTTCA CTTCCAGTGC 
CGCTGCAGAC GTGGATACCA GCTTACAGCA GATGGCAAGC GCTGTAGATT GAGGAACCCA 
TGTGTGGAGA AGAATGGCGG CTGTGTGCAT TTATGTCGCA GTGATGGCGG TCGGGTTGTG 
TGCAGCTGTC ACCAAGGCTT CATGCTAGCA ATGGACCAGA GGAGCTGTGA AGATGTCAAT 
GAGTGTGAAA CAGGACTGGC AGCATGTGCA CACAGTTGCA GGAACACTCC AGGATCGTTT 
TCCTGCATCT GCAATCCAGG ATACGAGCTG GGCTCTGATG GAAGGAAATG CTACCGTATT 
GAAATGGAGA TTGTCAATAC CTGTGACTCT GATAATGGTG GCTGTTCACA TCGCTGTCAG 
CATGGTGTTC TTGGGCCTGT CTGCAGCTGT AATCATGGTT ATCAGCTCGC CGAAGACCTC 
AAAACCTGTT TGGATCTGGA GGAATGTGAG GAAGGAACGT CATGCTGTGA GCAGGATTGC 
ACAAACTACC CTGGAGGGTA TGAATGCTAC TGCAGGGCAG GTTACAGACT CAATGCAGAT 
GGATGCGGAT GTGACGATGT GGACGAGTGT GCATCTGATA GAGGAGGCTG TGAACACACT 
TGTCAGAATA CGGCTGGCTC ATATCACTGC TTCTGTCGGT TTGGATATCG ACTTGGTGAG 
GACAGACACT CCTGCATTTC TCTCCAGCAA TCGGTTGAGG AATTGCCCGG TCCAGTGGTG 
GTTGAGCGTC CTCTCCTTCA TCTCGCTCTC CTCCAGGATT ACCCACAACC CCTGGAGCGC 
TATCATGACT ATGAGGAGGA CAGTTTTGGA GAGCTGCGGG CAGAGAGCAC CTTCGCTGAG 
AAGTTTGTGT GTTTGAATGG GACGTTTGGC CATGACTGCA GCCTGACATG TGATGATTGC 
TCAAATGGAG GATATTGTAA TGCTGAAGGA GATGCTTGTG ACTGTCCAGA TGGGTGGACG 
GGGATCCTCT GTAACCAAAC CTGTCCAGAG GGAACATTTG GTAAAAACTG CTCCTTCACT 
TGCAAGTGCA GAAACGGGGG CACCTGTGAC CCGATTAGTG GGAAGTGCCG CTGCCCACCT 
GGAGTCAATG GAGACCTGTG CCAGGATGGC TGTCCGAAGG GAATGTATGG AAAGCACTGC 
AACAAGAAAT GTAACTGTGC TAATAACGGC CGCTGCCATC GCACATATGG AGCCTGTCTG 
TGTGATCCAG GACTCTACGG CAGGTTCTGT CACCTCCCTT GCCCCAAGTG GTCGTATGGA 
TCTGGCTGCT CATCCGAGTG TCAGTGTGTG CAACAAAACA CGCTTGAGTG CCATCGACGT 
CATGGAACGT GTGTGTGCAA ACCTGGCTAT CAGGGGAAAA CCTGCAACCG CGAGTGTGAT 
AGTGGATTTT ATGGTCCAGG ATGTAGATTA AAATGCCAGT GTCCAGCGGG AGTCTCTTGC 
CATCCCATGA GTGGGCAATG TCAGCGTCAG TGCCCAGCAG GCCTACATGG GGAGCACTGT 
GACCGAGAGT GCCCCATGGG ACAGTTTGGA GTTGGATGCA CACAGTTTTG TGACTGTAAG 
GGAGCTCCAT GTAACTCAGT GACCGGGAAA TGCCACTGTC CTGCAGGGCA GACAGGACAA 
CACTGTGAAA AAGAGTGTGA AGAGGGCCGT TGGGGTCTTG GCTGTGCAGA ATCATGTCCT 
TCCTGTGACA ATGGTGGAGT ATGTGATAGA CAAAACGGCA CCTGTGTTTG TTCGCCGGGA 
TTCATGGGAA ATCTCTGTCA GAATGTCTGT CCAGATGGGC ACTTTGGCCT GAGCTGCCTG 
TTGCGCTGCT CCTGTCAAAA CGAAGGGAAA TGCCACCATG TGACTGGCCT CTGTGTTTGT 
AAAGACGGCT GGATGGGACC TAACTGCGCA AAAGCTTGTG TGGCTGGACG ATGGGGGCCG 
AACTGTGCCA ACCAGTGTGA CTGCAGGAGC AGCGTGGGCA GCTGTGACCC TGTGATTGGC 
CAGTGTCATT GTGAAGCGGG CTACATGGGA CCACGCTGTG ATCAAAAGTG TCCTAAGGGT 
TTCTTCGGCC AGAGCTGCCA GCACAGATGC CAGTGTGAGA ATGGCGCAGC GTGTGAGCAT 
GTGAGCGGTG CCTGCACTTG TTTGGCTGGC TGGACTGGGA CGTATTGTGA AAAACCATGT 
CCTGAGGGCT TTCATGGCTT GGAGTGCCAG CAGAAGTGCC AGTGCTTGAA TGGTGGCCGT 
TGTCATCCCG TGACTGGAGG CTGCCAGTGT CCATCTGGTT GGGTCGGGCC AGTTTGCAAT 
GCCACGTGTG AAGCTGGCAC CTTTGGGCCG GCCTGCAGTC AAACCTGTAG ATGCCACAAT 
AATGGCACCT GTGACCCTGC TGATGGACGA TGTGAGTGTG GAGCAGGATG GACAGGCAGC 
AGGTGTGAAC AAGAGTGCTC AGCTGGATTG TATGGTGTGG GCTGCAACCA GCATTGCCTG 
TGTCAGAATG GAGGGTCGTG TGACCGAATC AGTGGCCACT GCACATGTGT CAGTGGGTGG 
ACAGGAGTGG CCTGTGAATT GGAATGTGCT GCGGGACACT TTGGCATGGA CTGTCAGCAC 
AAGTGTGAGT GTGTAAATGG CGGCTTGTGT GACCGTCACA GTGGCAGGTG TTTGTGCCCA 
GCAGGTTGGA CAGGCACGCA CTGTGAGACA GCGTGCACTC AAGGTTTTTA TGGTCTGGGC 
TGTAAGGAGC GATGCCGGTG TGATCATAAC GCCGCATGCC ACCACATCAC TGGCACCTGC 
TTGTGTCCCG CTGGCTGGAG AGGGACTCAC TGCGAAAAGG TTTGTTTACC TGGTTCTTAT 
GGGAAGCGTT GTGCTCATCG ATGCCAGTGT CCTCATGGAA CCTCCTGTAA TCATGTGACT 
GGACAGTGTG GTTGCCCAGC GGGATTGACT GGTAGTGGCT GTGAGCGAAT GTGCCAGTCA 
GGTACGTTTG GGGTAAACTG TAATCAGGTG TGCCAGTGCT CTGAGCTCAA CCAGCTCTGC 
CACCCTGTCA CTGGTGTCTG TTACTGTGCT CCCGGATACT ATGGCAGCAA GTGTGAAACA 
GAATGTGAAG CGGGCCGTTA TGGACCCAAC TGCGAGCAGG AATGCCAATG TCTGAATAAC 
GGTGTATGTA AAAGCAGCAC TGGCACCTGC AGCTGCCCAC CGGGATTCAT CGGACCAAAC 
TGCAACATCA CGTGTCCATT AGGGCGTTAT GGTCAGGACT GTTTTGGAGT GTCTCTGTGT 
GGTGAAGGGG CTCGCAGTGA CCCTCTCACA GGCAGATGTG TGTGCAGGGC TGGTCAGCGT 
GGACGGGACT GTGGGAAAGG CTGTGCTGAG GGCTGGTTTG GAGAGAATTG TGATCAGCGC 
TGTAACTGCA GCAATGGAGG AGTGTGTGAC GCTCTTACAG GACACTGCAG CTGTACACTG 
GGCTGGACAG GAGACACCTG TGAGGAAGTG TGTCCCAGAG GACGGTATGG TTCAGACTGC 
AGTGAAGTGT GTGACTGTAA GAATAACAGC ACATGCGACC GAGTGACTGG CGTCTGTCTC 
TGCTCTCCAG GTTTCTACGG ACATCTATGT CAACATGCAT GTCCTGTTGG CTTCCATGGT 
CATCGTTGCC AGCTTCTGTG TGACTGTGGT GCAAATGCAC CTTGTGACCC TGAAACTGGC 
CGATGCCTCT GTCCTGCAGG ATACCAGGGA CTCCGCTGTG AGAGAGAGTG TGAATTGGGC 
ACATTCGGGC CTGATTGTGT CCACAGATGT GACTGTGATG GAGACACCCC ATGTGACCCT 
ATTAATGGCA GGTGTTTGTG TGCACCTGGA AAAACAGGCT CTCGCTGTGA CATAGGCTGT 
GGAGTGAACC GATTTGGACC CGACTGCTCA CAGAGATGTG AATGTCTGAA CGGAGGTCAG 
TGTGATCCCC GAAACGGCCG ATGCACGTGT CTGAACGGCT GGATCGGGCC CAGCTGTCAA 
CAAGGGCTCC TGTCTGCTAG CAAAAGTAAA AGGAAGGACT CGTCAGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329335.1
CDS	243..4613
Translation	MLSDWLRLVLVFTFWTQLVPSLAAGNPYYRYYHRNCYTCFNGYHAYNTGHQGADIDECQVHNGGCQHRCVNTRGSYYCECNPGFRLHVDGRTCIAVLSCSVNNGGCEHECVQLSPVHFQCRCRRGYQLTADGKRCRLRNPCVEKNGGCVHLCRSDGGRVVCSCHQGFMLAMDQRSCEDVNECETGLAACAHSCRNTPGSFSCICNPGYELGSDGRKCYRIEMEIVNTCDSDNGGCSHRCQHGVLGPVCSCNHGYQLAEDLKTCLDLEECEEGTSCCEQDCTNYPGGYECYCRAGYRLNADGCGCDDVDECASDRGGCEHTCQNTAGSYHCFCRFGYRLGEDRHSCISLQQSVEELPGPVVVERPLLHLALLQDYPQPLERYHDYEEDSFGELRAESTFAEKFVCLNGTFGHDCSLTCDDCSNGGYCNAEGDACDCPDGWTGILCNQTCPEGTFGKNCSFTCKCRNGGTCDPISGKCRCPPGVNGDLCQDGCPKGMYGKHCNKKCNCANNGRCHRTYGACLCDPGLYGRFCHLPCPKWSYGSGCSSECQCVQQNTLECHRRHGTCVCKPGYQGKTCNRECDSGFYGPGCRLKCQCPAGVSCHPMSGQCQRQCPAGLHGEHCDRECPMGQFGVGCTQFCDCKGAPCNSVTGKCHCPAGQTGQHCEKECEEGRWGLGCAESCPSCDNGGVCDRQNGTCVCSPGFMGNLCQNVCPDGHFGLSCLLRCSCQNEGKCHHVTGLCVCKDGWMGPNCAKACVAGRWGPNCANQCDCRSSVGSCDPVIGQCHCEAGYMGPRCDQKCPKGFFGQSCQHRCQCENGAACEHVSGACTCLAGWTGTYCEKPCPEGFHGLECQQKCQCLNGGRCHPVTGGCQCPSGWVGPVCNATCEAGTFGPACSQTCRCHNNGTCDPADGRCECGAGWTGSRCEQECSAGLYGVGCNQHCLCQNGGSCDRISGHCTCVSGWTGVACELECAAGHFGMDCQHKCECVNGGLCDRHSGRCLCPAGWTGTHCETACTQGFYGLGCKERCRCDHNAACHHITGTCLCPAGWRGTHCEKVCLPGSYGKRCAHRCQCPHGTSCNHVTGQCGCPAGLTGSGCERMCQSGTFGVNCNQVCQCSELNQLCHPVTGVCYCAPGYYGSKCETECEAGRYGPNCEQECQCLNNGVCKSSTGTCSCPPGFIGPNCNITCPLGRYGQDCFGVSLCGEGARSDPLTGRCVCRAGQRGRDCGKGCAEGWFGENCDQRCNCSNGGVCDALTGHCSCTLGWTGDTCEEVCPRGRYGSDCSEVCDCKNNSTCDRVTGVCLCSPGFYGHLCQHACPVGFHGHRCQLLCDCGANAPCDPETGRCLCPAGYQGLRCERECELGTFGPDCVHRCDCDGDTPCDPINGRCLCAPGKTGSRCDIGCGVNRFGPDCSQRCECLNGGQCDPRNGRCTCLNGWIGPSCQQGLLSASKSKRKDSSV

Target ORF information:

RefSeq Version	XM_021473660.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473660.1

ORF Insert Sequence:

ATGTTATCCG ATTGGTTGAG GTTGGTGCTT GTGTTTACCT TCTGGACTCA GCTGGTACCA 
AGTTTGGCTG CTGGAAATCC ATATTACCGC TACTATCACA GAAACTGCTA CACCTGCTTC 
AATGGATATC ATGCGTACAA CACAGGACAC CAGGGCGCAG ATATTGATGA GTGCCAGGTT 
CACAACGGAG GCTGCCAGCA CAGATGTGTG AACACTAGAG GCTCGTACTA CTGCGAGTGC 
AATCCAGGCT TTCGGCTCCA CGTTGATGGC CGCACCTGCA TAGCTGTTTT ATCCTGTTCG 
GTAAATAATG GAGGTTGTGA GCATGAATGT GTCCAGCTTT CTCCAGTTCA CTTCCAGTGC 
CGCTGCAGAC GTGGATACCA GCTTACAGCA GATGGCAAGC GCTGTAGATT GAGGAACCCA 
TGTGTGGAGA AGAATGGCGG CTGTGTGCAT TTATGTCGCA GTGATGGCGG TCGGGTTGTG 
TGCAGCTGTC ACCAAGGCTT CATGCTAGCA ATGGACCAGA GGAGCTGTGA AGATGTCAAT 
GAGTGTGAAA CAGGACTGGC AGCATGTGCA CACAGTTGCA GGAACACTCC AGGATCGTTT 
TCCTGCATCT GCAATCCAGG ATACGAGCTG GGCTCTGATG GAAGGAAATG CTACCGTATT 
GAAATGGAGA TTGTCAATAC CTGTGACTCT GATAATGGTG GCTGTTCACA TCGCTGTCAG 
CATGGTGTTC TTGGGCCTGT CTGCAGCTGT AATCATGGTT ATCAGCTCGC CGAAGACCTC 
AAAACCTGTT TGGATCTGGA GGAATGTGAG GAAGGAACGT CATGCTGTGA GCAGGATTGC 
ACAAACTACC CTGGAGGGTA TGAATGCTAC TGCAGGGCAG GTTACAGACT CAATGCAGAT 
GGATGCGGAT GTGACGATGT GGACGAGTGT GCATCTGATA GAGGAGGCTG TGAACACACT 
TGTCAGAATA CGGCTGGCTC ATATCACTGC TTCTGTCGGT TTGGATATCG ACTTGGTGAG 
GACAGACACT CCTGCATTTC TCTCCAGCAA TCGGTTGAGG AATTGCCCGG TCCAGTGGTG 
GTTGAGCGTC CTCTCCTTCA TCTCGCTCTC CTCCAGGATT ACCCACAACC CCTGGAGCGC 
TATCATGACT ATGAGGAGGA CAGTTTTGGA GAGCTGCGGG CAGAGAGCAC CTTCGCTGAG 
AAGTTTGTGT GTTTGAATGG GACGTTTGGC CATGACTGCA GCCTGACATG TGATGATTGC 
TCAAATGGAG GATATTGTAA TGCTGAAGGA GATGCTTGTG ACTGTCCAGA TGGGTGGACG 
GGGATCCTCT GTAACCAAAC CTGTCCAGAG GGAACATTTG GTAAAAACTG CTCCTTCACT 
TGCAAGTGCA GAAACGGGGG CACCTGTGAC CCGATTAGTG GGAAGTGCCG CTGCCCACCT 
GGAGTCAATG GAGACCTGTG CCAGGATGGC TGTCCGAAGG GAATGTATGG AAAGCACTGC 
AACAAGAAAT GTAACTGTGC TAATAACGGC CGCTGCCATC GCACATATGG AGCCTGTCTG 
TGTGATCCAG GACTCTACGG CAGGTTCTGT CACCTCCCTT GCCCCAAGTG GTCGTATGGA 
TCTGGCTGCT CATCCGAGTG TCAGTGTGTG CAACAAAACA CGCTTGAGTG CCATCGACGT 
CATGGAACGT GTGTGTGCAA ACCTGGCTAT CAGGGGAAAA CCTGCAACCG CGAGTGTGAT 
AGTGGATTTT ATGGTCCAGG ATGTAGATTA AAATGCCAGT GTCCAGCGGG AGTCTCTTGC 
CATCCCATGA GTGGGCAATG TCAGCGTCAG TGCCCAGCAG GCCTACATGG GGAGCACTGT 
GACCGAGAGT GCCCCATGGG ACAGTTTGGA GTTGGATGCA CACAGTTTTG TGACTGTAAG 
GGAGCTCCAT GTAACTCAGT GACCGGGAAA TGCCACTGTC CTGCAGGGCA GACAGGACAA 
CACTGTGAAA AAGAGTGTGA AGAGGGCCGT TGGGGTCTTG GCTGTGCAGA ATCATGTCCT 
TCCTGTGACA ATGGTGGAGT ATGTGATAGA CAAAACGGCA CCTGTGTTTG TTCGCCGGGA 
TTCATGGGAA ATCTCTGTCA GAATGTCTGT CCAGATGGGC ACTTTGGCCT GAGCTGCCTG 
TTGCGCTGCT CCTGTCAAAA CGAAGGGAAA TGCCACCATG TGACTGGCCT CTGTGTTTGT 
AAAGACGGCT GGATGGGACC TAACTGCGCA AAAGCTTGTG TGGCTGGACG ATGGGGGCCG 
AACTGTGCCA ACCAGTGTGA CTGCAGGAGC AGCGTGGGCA GCTGTGACCC TGTGATTGGC 
CAGTGTCATT GTGAAGCGGG CTACATGGGA CCACGCTGTG ATCAAAAGTG TCCTAAGGGT 
TTCTTCGGCC AGAGCTGCCA GCACAGATGC CAGTGTGAGA ATGGCGCAGC GTGTGAGCAT 
GTGAGCGGTG CCTGCACTTG TTTGGCTGGC TGGACTGGGA CGTATTGTGA AAAACCATGT 
CCTGAGGGCT TTCATGGCTT GGAGTGCCAG CAGAAGTGCC AGTGCTTGAA TGGTGGCCGT 
TGTCATCCCG TGACTGGAGG CTGCCAGTGT CCATCTGGTT GGGTCGGGCC AGTTTGCAAT 
GCCACGTGTG AAGCTGGCAC CTTTGGGCCG GCCTGCAGTC AAACCTGTAG ATGCCACAAT 
AATGGCACCT GTGACCCTGC TGATGGACGA TGTGAGTGTG GAGCAGGATG GACAGGCAGC 
AGGTGTGAAC AAGAGTGCTC AGCTGGATTG TATGGTGTGG GCTGCAACCA GCATTGCCTG 
TGTCAGAATG GAGGGTCGTG TGACCGAATC AGTGGCCACT GCACATGTGT CAGTGGGTGG 
ACAGGAGTGG CCTGTGAATT GGAATGTGCT GCGGGACACT TTGGCATGGA CTGTCAGCAC 
AAGTGTGAGT GTGTAAATGG CGGCTTGTGT GACCGTCACA GTGGCAGGTG TTTGTGCCCA 
GCAGGTTGGA CAGGCACGCA CTGTGAGACA GCGTGCACTC AAGGTTTTTA TGGTCTGGGC 
TGTAAGGAGC GATGCCGGTG TGATCATAAC GCCGCATGCC ACCACATCAC TGGCACCTGC 
TTGTGTCCCG CTGGCTGGAG AGGGACTCAC TGCGAAAAGG TTTGTTTACC TGGTTCTTAT 
GGGAAGCGTT GTGCTCATCG ATGCCAGTGT CCTCATGGAA CCTCCTGTAA TCATGTGACT 
GGACAGTGTG GTTGCCCAGC GGGATTGACT GGTAGTGGCT GTGAGCGAAT GTGCCAGTCA 
GGTACGTTTG GGGTAAACTG TAATCAGGTG TGCCAGTGCT CTGAGCTCAA CCAGCTCTGC 
CACCCTGTCA CTGGTGTCTG TTACTGTGCT CCCGGATACT ATGGCAGCAA GTGTGAAACA 
GAATGTGAAG CGGGCCGTTA TGGACCCAAC TGCGAGCAGG AATGCCAATG TCTGAATAAC 
GGTGTATGTA AAAGCAGCAC TGGCACCTGC AGCTGCCCAC CGGGATTCAT CGGACCAAAC 
TGCAACATCA CGTGTCCATT AGGGCGTTAT GGTCAGGACT GTTTTGGAGT GTCTCTGTGT 
GGTGAAGGGG CTCGCAGTGA CCCTCTCACA GGCAGATGTG TGTGCAGGGC TGGTCAGCGT 
GGACGGGACT GTGGGAAAGG CTGTGCTGAG GGCTGGTTTG GAGAGAATTG TGATCAGCGC 
TGTAACTGCA GCAATGGAGG AGTGTGTGAC GCTCTTACAG GACACTGCAG CTGTACACTG 
GGCTGGACAG GAGACACCTG TGAGGAAGTG TGTCCCAGAG GACGGTATGG TTCAGACTGC 
AGTGAAGTGT GTGACTGTAA GAATAACAGC ACATGCGACC GAGTGACTGG CGTCTGTCTC 
TGCTCTCCAG GTTTCTACGG ACATCTATGT CAACATGCAT GTCCTGTTGG CTTCCATGGT 
CATCGTTGCC AGCTTCTGTG TGACTGTGGT GCAAATGCAC CTTGTGACCC TGAAACTGGC 
CGATGCCTCT GTCCTGCAGG ATACCAGGGA CTCCGCTGTG AGAGAGAGTG TGAATTGGGC 
ACATTCGGGC CTGATTGTGT CCACAGATGT GACTGTGATG GAGACACCCC ATGTGACCCT 
ATTAATGGCA GGTGTTTGTG TGCACCTGGA AAAACAGGCT CTCGCTGTGA CATAGGCTGT 
GGAGTGAACC GATTTGGACC CGACTGCTCA CAGAGATGTG AATGTCTGAA CGGAGGTCAG 
TGTGATCCCC GAAACGGCCG ATGCACGTGT CTGAACGGCT GGATCGGGCC CAGCTGTCAA 
CAAGGGCTCC TGTCTGCTAG CAAAAGTAAA AGGAAGGACT CGTCAGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

megf6a ( XM_017353739.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209228.1 Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa57519
Clone ID Related Accession (Same CDS sequence)	XM_017353739.1
Accession Version	XM_017353739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	multiple epidermal growth factor-like domains protein 6 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGG TCTGGAGGCA AGGATGCACA GGTCATGCTT GGTGTATGGA CTTCGAGAGG 
AGGACTACCT ACTACATAGG ATATAAACAA GTCTACAGGC AGGACTTCAA GATGGCATAC 
AAATGCTGTC CTGGATGGTC CCAGAACAAT TCAAAGATGG GCTGCCTTTA TCATATTGAT 
GAGTGCCAGG TTCACAACGG AGGCTGCCAG CACAGATGTG TGAACACTAG AGGCTCGTAC 
TACTGCGAGT GCAATCCAGG CTTTCGGCTC CACGTTGATG GCCGCACCTG CATAGCTGTT 
TTATCCTGTT CGGTAAATAA TGGAGGTTGT GAGCATGAAT GTGTCCAGCT TTCTCCAGTT 
CACTTCCAGT GCCGCTGCAG ACGTGGATAC CAGCTTACAG CAGATGGCAA GCGCTGTAGA 
TTGAGGAACC CATGTGTGGA GAAGAATGGC GGCTGTGTGC ATTTATGTCG CAGTGATGGT 
GGTCGGGTTG TGTGCAGCTG TCACCAAGGC TTCATGCTAG CAGTGGACCA GAAGAGCTGT 
GAAGATGTCA ATGAGTGTGA AACAGGACTG GCAGCATGTG CACACAGTTG CAGGAACACT 
CCAGGATCGT TTTCCTGCAT CTGCAATCCA GGATACGAGC TGGGCTCTGA TGGAAGGAAA 
TGCTACCGTA TTGAAATGGA GATTGTCAAT ACCTGTGACT CTGATAATGG TGGCTGTTCA 
CATCGCTGTC AGCATGGTGT TCTTGGGCCT GTCTGCAGCT GTAATCATGG TTATCAGCTC 
GCCGAAGACC TCAAAACCTG TTTGGATCTG GAGGAATGTG AGGAAGGAAC GTCATGCTGT 
GAGCAGGATT GCACAAACTA CCCTGGAGGG TATGAATGCT ACTGCAGGGC AGGTTACAGA 
CTCAATGCAG ATGGATGCGG ATGTGACGAT GTGGACGAGT GTGCGTCTGA TAGAGGAGGC 
TGTGAACACA CTTGTCAGAA TACGGCTGGC TCATATCACT GCTTCTGTCG GTTTGGATAT 
CGACTTGGTG AGGACAGACA CTCCTGCATT TCTCTCCAGC GATCGGTTGA GGAATTGCCC 
GGTCCAGTGG TGGTTGAGCG TCCTCTCCTT CATCTCGCTC TCCTCCAGGA TTACCCACAA 
CCCCTGGAGC GCTATCATGA CTATGAGGAG GACAGTTTTG GAGAGCTGCG GGCAGAGAGC 
ACCTTCGCTG AGAAGTTTGT GTGTTTGAAT GGGACGTTTG GCCATGACTG CAGCCTGACG 
TGTGATGATT GCTCAAATGG AGGATATTGT AATGCTGAAG GAGATGCTTG TGACTGTCCA 
GATGGGTGGA CGGGGATCCT TTGTAACCAA ACCTGTCCAG AGGGAACATT TGGTAAAAAC 
TGCTCCTTCA CTTGCAAGTG CAGAAACGGG GGCACCTGTG ACCCGATTAG TGGGAAGTGC 
CGCTGCCCAC CTGGAGTCAA TGGAGACCTG TGCCAGGACG GCTGTCCGAA GGGAATGTAT 
GGAAAGCACT GCAACAAGAA ATGTAACTGT GCTAATAACG GCCGCTGCCA TCGCACATAT 
GGAGCCTGTC TGTGTGATCC AGGACTCTAC GGCAGGTTCT GTCACCTCCC TTGCCCCAAG 
TGGTCGTATG GATCTGGCTG CTCATCCGAG TGTCAGTGTG TGCAACAAAA CACGCTTGAG 
TGCCATCGAC GTCATGGAAC GTGTGTGTGC AAACCTGGCT ATCAGGGGAA AACCTGCAAC 
CGCGAGTGTG ATAGTGGATT TTATGGTCCA GGATGTAGAT TAAAATGCCA GTGTCCAGCG 
GGAGTCTCTT GCCATCCCAT GAGTGGGCAA TGTCAGCGTC AGTGCCCAGC AGGCCTACAT 
GGGGAGCACT GTGACCGAGA GTGCCCCATG GGACAGTTTG GAGTTGGATG CACACAGTTT 
TGTGACTGTA AGGGAGCTCC ATGTAACTCA GTGACCGGGA AATGCCACTG TCCTGCAGGG 
CAGACAGGAC AACACTGTGA AAAAGAGTGT GAAGAGGGCC GTTGGGGTCT TGGCTGTGCA 
GAATCATGTC CTTCCTGTGA CAATGGTGGA GTATGTGATA GACAAAACGG CACCTGTGTT 
TGTTCGCCGG GATTCATGGG AAATCTCTGT CAGAATGTCT GTCCAGATGG GCACTTTGGC 
CTGAGCTGCC TGTTGCGCTG CTCCTGTCAA AACGAAGGGA AATGCCACCA TGTGACTGGC 
CTCTGTGTTT GTAAAGATGG CTGGATGGGA CCCAACTGCG CAAAAGCTTG TGTGGCTGGA 
CGATGGGGGC CGAACTGTGC CAACCAGTGT GACTGCAGGA GCAGCGTGGG CAGCTGTGAC 
CCTGTGATTG GCCTGTGTCA TTGTGAAGCG GGCTACATGG GACCACGCTG TGATCAAAAG 
TGTCCTCAGG GTTTCTTCGG CCAGAGCTGC CAGCACAGAT GCCAGTGTGA GAATGGCGCA 
GTGTGTGAGC ATGTGAGCGG TGCCTGCACT TGTTTGGCTG GCTGGACTGG GACGTATTGT 
GAAAAACCTT GTCCTGAGGG CTTTCATGGC TTGGAGTGCC AGCAGAAGTG CCAGTGCTTG 
AATGGTGGCC GTTGTCATCC CGTGACTGGA GGCTGCCAGT GTCCATCTGG TTGGGTCGGG 
CCAGTTTGCA ATGCCACGTG TGAAGCTGGC ACCTTTGGGC CGGCCTGCAG TCAAACCTGT 
AGATGCCACA ATAATGGCAC CTGTGACCCT GCTGATGGGC GATGTGAGTG TGGAGCAGGA 
TGGACAGGCA GCAGCTGTGA ACAAGAGTGC TCAGCTGGAT TGTATGGTGT GGGCTGCAAC 
CAGCATTGCC TGTGTCAGAA TGGAGGGTCG TGTGACCGAA TCAGTGGCCA CTGCACATGT 
GTCAGTGGGT GGACAGGAGT GGCCTGTGAA TTGGAATGTG CTGCGGGACA CTTTGGCATG 
GACTGTCAGC ACAAGTGTGA GTGTGTAAAT GGCGGCGTGT GTGACCGTCA CAGTGGCAGG 
TGTTTGTGCC CAGCAGGTTG GACAGGCACG CACTGTGAGA CAGCATGCAC TCAAGGTTTT 
TATGGTCCGG GCTGTAAGGA GCGATGCCGG TGTGATCATA ACGCCGCATG CCACCACATC 
ACTGGCACCT GCTTGTGTCC CGCTGGCTGG AGAGGGACTC ACTGCGAAAA GGTTTGTTTA 
CCTGGTTCCT ATGGGAAGCG TTGTGCTCAT CGATGCCAGT GTCCTCATGG AACCTCCTGT 
AACCATGTGA CTGGACAGTG TGGTTGCCCA GCGGGATTAA CTGGTAGTGG CTGTGAGCGA 
ATGTGCCAGT CAGGTACGTT TGGGGTAAAC TGTAATCAGG TGTGCCAGTG CTCTGAGCTC 
AACCAGCTCT GCCACCCCGT CACTGGTGTC TGTTACTGTG CTCCCGGATA CTATGGCAGC 
AAGTGTGAAA CAGAATGTGA AGCGGGCCGT TATGGACCCA ACTGTGAGCA GGAATGCCAG 
TGTCTGAATA ACGGTGTATG TAAAAGCAGC ACTGGCACCT GCAGCTGCCC ACCGGGATTC 
ATCGGACCAA ACTGCAACAT CACATGTCCA TTAGGGCGTT ATGGTCAGGA CTGTGCTGGA 
GTGTCTCTGT GTGGTGAAGG GGCTCGCAGT GACCCTCTCA CAGGCAGATG TGTGTGCAGG 
GCTGGTCAGC GTGGACGGGA CTGTGGGAAA GGCTGTGCTG AGGGCTGGTT TGGAGAGAAT 
TGTGATCAGC GCTGTAACTG CAGCAATGGC GGAGTGTGTG ACGCTCTTAC AGGACACTGC 
AGCTGTACAC TGGGCTGGAC AGGAGACACC TGTGAGGAAG TGTGTCCCAG AGGACGGTAT 
GGTTCAGACT GCAGTGAAGT GTGTGACTGT AAGAATAACA GCACATGCGA CCGAGTGACT 
GGCGTCTGTC TCTGCTCTCC AGGTTTCTAC GGACATCTAT GTCAACATGC ATGTCCTGTT 
GGCTTCCATG GTCATCGTTG CCAGCTTCTG TGTGACTGTG GTGCAAATGC ACCTTGTGAC 
CCTGAAACTG GCCGATGCCT CTGTCCTGCA GGATACCAGG GACTCCGCTG TGAGAGAGAG 
TGTGAATTGG GCACATTTGG GGCTGATTGT GTCCACAGAT GTGACTGTGA TGGAGACACC 
CCATGTGACC CTATTAATGG CAGGTGTTTG TGTGCACCTG GAAAAACAGG CTCTCGCTGT 
GACATAGGCT GTGGAGTGAA CCGATTTGGA CCCGACTGCT CACAGAGATG TGAATGTCTG 
AACGGAGGTC AGTGTGATCC CCGAAACGGC CGATGCACGT GTCTGAACGG CTGGATCGGG 
CCCAGCTGTC AACAAGGGCT CCTGTCTGCT AGCAAAAGTA AAAGGAAGGA CTCGTCAGTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209228.1
CDS	57..4439
Translation	MVKVWRQGCTGHAWCMDFERRTTYYIGYKQVYRQDFKMAYKCCPGWSQNNSKMGCLYHIDECQVHNGGCQHRCVNTRGSYYCECNPGFRLHVDGRTCIAVLSCSVNNGGCEHECVQLSPVHFQCRCRRGYQLTADGKRCRLRNPCVEKNGGCVHLCRSDGGRVVCSCHQGFMLAVDQKSCEDVNECETGLAACAHSCRNTPGSFSCICNPGYELGSDGRKCYRIEMEIVNTCDSDNGGCSHRCQHGVLGPVCSCNHGYQLAEDLKTCLDLEECEEGTSCCEQDCTNYPGGYECYCRAGYRLNADGCGCDDVDECASDRGGCEHTCQNTAGSYHCFCRFGYRLGEDRHSCISLQRSVEELPGPVVVERPLLHLALLQDYPQPLERYHDYEEDSFGELRAESTFAEKFVCLNGTFGHDCSLTCDDCSNGGYCNAEGDACDCPDGWTGILCNQTCPEGTFGKNCSFTCKCRNGGTCDPISGKCRCPPGVNGDLCQDGCPKGMYGKHCNKKCNCANNGRCHRTYGACLCDPGLYGRFCHLPCPKWSYGSGCSSECQCVQQNTLECHRRHGTCVCKPGYQGKTCNRECDSGFYGPGCRLKCQCPAGVSCHPMSGQCQRQCPAGLHGEHCDRECPMGQFGVGCTQFCDCKGAPCNSVTGKCHCPAGQTGQHCEKECEEGRWGLGCAESCPSCDNGGVCDRQNGTCVCSPGFMGNLCQNVCPDGHFGLSCLLRCSCQNEGKCHHVTGLCVCKDGWMGPNCAKACVAGRWGPNCANQCDCRSSVGSCDPVIGLCHCEAGYMGPRCDQKCPQGFFGQSCQHRCQCENGAVCEHVSGACTCLAGWTGTYCEKPCPEGFHGLECQQKCQCLNGGRCHPVTGGCQCPSGWVGPVCNATCEAGTFGPACSQTCRCHNNGTCDPADGRCECGAGWTGSSCEQECSAGLYGVGCNQHCLCQNGGSCDRISGHCTCVSGWTGVACELECAAGHFGMDCQHKCECVNGGVCDRHSGRCLCPAGWTGTHCETACTQGFYGPGCKERCRCDHNAACHHITGTCLCPAGWRGTHCEKVCLPGSYGKRCAHRCQCPHGTSCNHVTGQCGCPAGLTGSGCERMCQSGTFGVNCNQVCQCSELNQLCHPVTGVCYCAPGYYGSKCETECEAGRYGPNCEQECQCLNNGVCKSSTGTCSCPPGFIGPNCNITCPLGRYGQDCAGVSLCGEGARSDPLTGRCVCRAGQRGRDCGKGCAEGWFGENCDQRCNCSNGGVCDALTGHCSCTLGWTGDTCEEVCPRGRYGSDCSEVCDCKNNSTCDRVTGVCLCSPGFYGHLCQHACPVGFHGHRCQLLCDCGANAPCDPETGRCLCPAGYQGLRCERECELGTFGADCVHRCDCDGDTPCDPINGRCLCAPGKTGSRCDIGCGVNRFGPDCSQRCECLNGGQCDPRNGRCTCLNGWIGPSCQQGLLSASKSKRKDSSV

Target ORF information:

RefSeq Version	XM_017353739.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353739.1

ORF Insert Sequence:

ATGGTGAAGG TCTGGAGGCA AGGATGCACA GGTCATGCTT GGTGTATGGA CTTCGAGAGG 
AGGACTACCT ACTACATAGG ATATAAACAA GTCTACAGGC AGGACTTCAA GATGGCATAC 
AAATGCTGTC CTGGATGGTC CCAGAACAAT TCAAAGATGG GCTGCCTTTA TCATATTGAT 
GAGTGCCAGG TTCACAACGG AGGCTGCCAG CACAGATGTG TGAACACTAG AGGCTCGTAC 
TACTGCGAGT GCAATCCAGG CTTTCGGCTC CACGTTGATG GCCGCACCTG CATAGCTGTT 
TTATCCTGTT CGGTAAATAA TGGAGGTTGT GAGCATGAAT GTGTCCAGCT TTCTCCAGTT 
CACTTCCAGT GCCGCTGCAG ACGTGGATAC CAGCTTACAG CAGATGGCAA GCGCTGTAGA 
TTGAGGAACC CATGTGTGGA GAAGAATGGC GGCTGTGTGC ATTTATGTCG CAGTGATGGT 
GGTCGGGTTG TGTGCAGCTG TCACCAAGGC TTCATGCTAG CAGTGGACCA GAAGAGCTGT 
GAAGATGTCA ATGAGTGTGA AACAGGACTG GCAGCATGTG CACACAGTTG CAGGAACACT 
CCAGGATCGT TTTCCTGCAT CTGCAATCCA GGATACGAGC TGGGCTCTGA TGGAAGGAAA 
TGCTACCGTA TTGAAATGGA GATTGTCAAT ACCTGTGACT CTGATAATGG TGGCTGTTCA 
CATCGCTGTC AGCATGGTGT TCTTGGGCCT GTCTGCAGCT GTAATCATGG TTATCAGCTC 
GCCGAAGACC TCAAAACCTG TTTGGATCTG GAGGAATGTG AGGAAGGAAC GTCATGCTGT 
GAGCAGGATT GCACAAACTA CCCTGGAGGG TATGAATGCT ACTGCAGGGC AGGTTACAGA 
CTCAATGCAG ATGGATGCGG ATGTGACGAT GTGGACGAGT GTGCGTCTGA TAGAGGAGGC 
TGTGAACACA CTTGTCAGAA TACGGCTGGC TCATATCACT GCTTCTGTCG GTTTGGATAT 
CGACTTGGTG AGGACAGACA CTCCTGCATT TCTCTCCAGC GATCGGTTGA GGAATTGCCC 
GGTCCAGTGG TGGTTGAGCG TCCTCTCCTT CATCTCGCTC TCCTCCAGGA TTACCCACAA 
CCCCTGGAGC GCTATCATGA CTATGAGGAG GACAGTTTTG GAGAGCTGCG GGCAGAGAGC 
ACCTTCGCTG AGAAGTTTGT GTGTTTGAAT GGGACGTTTG GCCATGACTG CAGCCTGACG 
TGTGATGATT GCTCAAATGG AGGATATTGT AATGCTGAAG GAGATGCTTG TGACTGTCCA 
GATGGGTGGA CGGGGATCCT TTGTAACCAA ACCTGTCCAG AGGGAACATT TGGTAAAAAC 
TGCTCCTTCA CTTGCAAGTG CAGAAACGGG GGCACCTGTG ACCCGATTAG TGGGAAGTGC 
CGCTGCCCAC CTGGAGTCAA TGGAGACCTG TGCCAGGACG GCTGTCCGAA GGGAATGTAT 
GGAAAGCACT GCAACAAGAA ATGTAACTGT GCTAATAACG GCCGCTGCCA TCGCACATAT 
GGAGCCTGTC TGTGTGATCC AGGACTCTAC GGCAGGTTCT GTCACCTCCC TTGCCCCAAG 
TGGTCGTATG GATCTGGCTG CTCATCCGAG TGTCAGTGTG TGCAACAAAA CACGCTTGAG 
TGCCATCGAC GTCATGGAAC GTGTGTGTGC AAACCTGGCT ATCAGGGGAA AACCTGCAAC 
CGCGAGTGTG ATAGTGGATT TTATGGTCCA GGATGTAGAT TAAAATGCCA GTGTCCAGCG 
GGAGTCTCTT GCCATCCCAT GAGTGGGCAA TGTCAGCGTC AGTGCCCAGC AGGCCTACAT 
GGGGAGCACT GTGACCGAGA GTGCCCCATG GGACAGTTTG GAGTTGGATG CACACAGTTT 
TGTGACTGTA AGGGAGCTCC ATGTAACTCA GTGACCGGGA AATGCCACTG TCCTGCAGGG 
CAGACAGGAC AACACTGTGA AAAAGAGTGT GAAGAGGGCC GTTGGGGTCT TGGCTGTGCA 
GAATCATGTC CTTCCTGTGA CAATGGTGGA GTATGTGATA GACAAAACGG CACCTGTGTT 
TGTTCGCCGG GATTCATGGG AAATCTCTGT CAGAATGTCT GTCCAGATGG GCACTTTGGC 
CTGAGCTGCC TGTTGCGCTG CTCCTGTCAA AACGAAGGGA AATGCCACCA TGTGACTGGC 
CTCTGTGTTT GTAAAGATGG CTGGATGGGA CCCAACTGCG CAAAAGCTTG TGTGGCTGGA 
CGATGGGGGC CGAACTGTGC CAACCAGTGT GACTGCAGGA GCAGCGTGGG CAGCTGTGAC 
CCTGTGATTG GCCTGTGTCA TTGTGAAGCG GGCTACATGG GACCACGCTG TGATCAAAAG 
TGTCCTCAGG GTTTCTTCGG CCAGAGCTGC CAGCACAGAT GCCAGTGTGA GAATGGCGCA 
GTGTGTGAGC ATGTGAGCGG TGCCTGCACT TGTTTGGCTG GCTGGACTGG GACGTATTGT 
GAAAAACCTT GTCCTGAGGG CTTTCATGGC TTGGAGTGCC AGCAGAAGTG CCAGTGCTTG 
AATGGTGGCC GTTGTCATCC CGTGACTGGA GGCTGCCAGT GTCCATCTGG TTGGGTCGGG 
CCAGTTTGCA ATGCCACGTG TGAAGCTGGC ACCTTTGGGC CGGCCTGCAG TCAAACCTGT 
AGATGCCACA ATAATGGCAC CTGTGACCCT GCTGATGGGC GATGTGAGTG TGGAGCAGGA 
TGGACAGGCA GCAGCTGTGA ACAAGAGTGC TCAGCTGGAT TGTATGGTGT GGGCTGCAAC 
CAGCATTGCC TGTGTCAGAA TGGAGGGTCG TGTGACCGAA TCAGTGGCCA CTGCACATGT 
GTCAGTGGGT GGACAGGAGT GGCCTGTGAA TTGGAATGTG CTGCGGGACA CTTTGGCATG 
GACTGTCAGC ACAAGTGTGA GTGTGTAAAT GGCGGCGTGT GTGACCGTCA CAGTGGCAGG 
TGTTTGTGCC CAGCAGGTTG GACAGGCACG CACTGTGAGA CAGCATGCAC TCAAGGTTTT 
TATGGTCCGG GCTGTAAGGA GCGATGCCGG TGTGATCATA ACGCCGCATG CCACCACATC 
ACTGGCACCT GCTTGTGTCC CGCTGGCTGG AGAGGGACTC ACTGCGAAAA GGTTTGTTTA 
CCTGGTTCCT ATGGGAAGCG TTGTGCTCAT CGATGCCAGT GTCCTCATGG AACCTCCTGT 
AACCATGTGA CTGGACAGTG TGGTTGCCCA GCGGGATTAA CTGGTAGTGG CTGTGAGCGA 
ATGTGCCAGT CAGGTACGTT TGGGGTAAAC TGTAATCAGG TGTGCCAGTG CTCTGAGCTC 
AACCAGCTCT GCCACCCCGT CACTGGTGTC TGTTACTGTG CTCCCGGATA CTATGGCAGC 
AAGTGTGAAA CAGAATGTGA AGCGGGCCGT TATGGACCCA ACTGTGAGCA GGAATGCCAG 
TGTCTGAATA ACGGTGTATG TAAAAGCAGC ACTGGCACCT GCAGCTGCCC ACCGGGATTC 
ATCGGACCAA ACTGCAACAT CACATGTCCA TTAGGGCGTT ATGGTCAGGA CTGTGCTGGA 
GTGTCTCTGT GTGGTGAAGG GGCTCGCAGT GACCCTCTCA CAGGCAGATG TGTGTGCAGG 
GCTGGTCAGC GTGGACGGGA CTGTGGGAAA GGCTGTGCTG AGGGCTGGTT TGGAGAGAAT 
TGTGATCAGC GCTGTAACTG CAGCAATGGC GGAGTGTGTG ACGCTCTTAC AGGACACTGC 
AGCTGTACAC TGGGCTGGAC AGGAGACACC TGTGAGGAAG TGTGTCCCAG AGGACGGTAT 
GGTTCAGACT GCAGTGAAGT GTGTGACTGT AAGAATAACA GCACATGCGA CCGAGTGACT 
GGCGTCTGTC TCTGCTCTCC AGGTTTCTAC GGACATCTAT GTCAACATGC ATGTCCTGTT 
GGCTTCCATG GTCATCGTTG CCAGCTTCTG TGTGACTGTG GTGCAAATGC ACCTTGTGAC 
CCTGAAACTG GCCGATGCCT CTGTCCTGCA GGATACCAGG GACTCCGCTG TGAGAGAGAG 
TGTGAATTGG GCACATTTGG GGCTGATTGT GTCCACAGAT GTGACTGTGA TGGAGACACC 
CCATGTGACC CTATTAATGG CAGGTGTTTG TGTGCACCTG GAAAAACAGG CTCTCGCTGT 
GACATAGGCT GTGGAGTGAA CCGATTTGGA CCCGACTGCT CACAGAGATG TGAATGTCTG 
AACGGAGGTC AGTGTGATCC CCGAAACGGC CGATGCACGT GTCTGAACGG CTGGATCGGG 
CCCAGCTGTC AACAAGGGCT CCTGTCTGCT AGCAAAAGTA AAAGGAAGGA CTCGTCAGTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

megf6a ( XM_021473659.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329334.1 Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X1, mRNA.

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CloneID	ODa146260
Clone ID Related Accession (Same CDS sequence)	XM_021473659.1
Accession Version	XM_021473659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	multiple epidermal growth factor-like domains protein 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395144.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAC ACGTGTTGTT GTTATTATTA TTCCTGCTAA CCAAGATCCG TCCATCGTGT 
TGTCTTCGAG CTCTACATCC TTTCATGCCG AATGTTTGCT CAGAGCCAGA GAGAGTCATC 
CGAGATGAGA AACAGCCATG TGTGCAGGCA TTTATACAAA TGGTGAAGGT CTGGAGGCAA 
GGATGCACAG GTCATGCTTG GTGTATGGAC TTCGAGAGGA GGACTACCTA CTACATAGGA 
TATAAACAAG TCTACAGGCA GGACTTCAAG ATGGCATACA AATGCTGTCC TGGATGGTCC 
CAGAACAATT CAAAGATGGG CTGCCTTTAT CATATTGATG AGTGCCAGGT TCACAACGGA 
GGCTGCCAGC ACAGATGTGT GAACACTAGA GGCTCGTACT ACTGCGAGTG CAATCCAGGC 
TTTCGGCTCC ACGTTGATGG CCGCACCTGC ATAGCTGTTT TATCCTGTTC GGTAAATAAT 
GGAGGTTGTG AGCATGAATG TGTCCAGCTT TCTCCAGTTC ACTTCCAGTG CCGCTGCAGA 
CGTGGATACC AGCTTACAGC AGATGGCAAG CGCTGTAGAT TGAGGAACCC ATGTGTGGAG 
AAGAATGGCG GCTGTGTGCA TTTATGTCGC AGTGATGGCG GTCGGGTTGT GTGCAGCTGT 
CACCAAGGCT TCATGCTAGC AATGGACCAG AGGAGCTGTG AAGATGTCAA TGAGTGTGAA 
ACAGGACTGG CAGCATGTGC ACACAGTTGC AGGAACACTC CAGGATCGTT TTCCTGCATC 
TGCAATCCAG GATACGAGCT GGGCTCTGAT GGAAGGAAAT GCTACCGTAT TGAAATGGAG 
ATTGTCAATA CCTGTGACTC TGATAATGGT GGCTGTTCAC ATCGCTGTCA GCATGGTGTT 
CTTGGGCCTG TCTGCAGCTG TAATCATGGT TATCAGCTCG CCGAAGACCT CAAAACCTGT 
TTGGATCTGG AGGAATGTGA GGAAGGAACG TCATGCTGTG AGCAGGATTG CACAAACTAC 
CCTGGAGGGT ATGAATGCTA CTGCAGGGCA GGTTACAGAC TCAATGCAGA TGGATGCGGA 
TGTGACGATG TGGACGAGTG TGCATCTGAT AGAGGAGGCT GTGAACACAC TTGTCAGAAT 
ACGGCTGGCT CATATCACTG CTTCTGTCGG TTTGGATATC GACTTGGTGA GGACAGACAC 
TCCTGCATTT CTCTCCAGCA ATCGGTTGAG GAATTGCCCG GTCCAGTGGT GGTTGAGCGT 
CCTCTCCTTC ATCTCGCTCT CCTCCAGGAT TACCCACAAC CCCTGGAGCG CTATCATGAC 
TATGAGGAGG ACAGTTTTGG AGAGCTGCGG GCAGAGAGCA CCTTCGCTGA GAAGTTTGTG 
TGTTTGAATG GGACGTTTGG CCATGACTGC AGCCTGACAT GTGATGATTG CTCAAATGGA 
GGATATTGTA ATGCTGAAGG AGATGCTTGT GACTGTCCAG ATGGGTGGAC GGGGATCCTC 
TGTAACCAAA CCTGTCCAGA GGGAACATTT GGTAAAAACT GCTCCTTCAC TTGCAAGTGC 
AGAAACGGGG GCACCTGTGA CCCGATTAGT GGGAAGTGCC GCTGCCCACC TGGAGTCAAT 
GGAGACCTGT GCCAGGATGG CTGTCCGAAG GGAATGTATG GAAAGCACTG CAACAAGAAA 
TGTAACTGTG CTAATAACGG CCGCTGCCAT CGCACATATG GAGCCTGTCT GTGTGATCCA 
GGACTCTACG GCAGGTTCTG TCACCTCCCT TGCCCCAAGT GGTCGTATGG ATCTGGCTGC 
TCATCCGAGT GTCAGTGTGT GCAACAAAAC ACGCTTGAGT GCCATCGACG TCATGGAACG 
TGTGTGTGCA AACCTGGCTA TCAGGGGAAA ACCTGCAACC GCGAGTGTGA TAGTGGATTT 
TATGGTCCAG GATGTAGATT AAAATGCCAG TGTCCAGCGG GAGTCTCTTG CCATCCCATG 
AGTGGGCAAT GTCAGCGTCA GTGCCCAGCA GGCCTACATG GGGAGCACTG TGACCGAGAG 
TGCCCCATGG GACAGTTTGG AGTTGGATGC ACACAGTTTT GTGACTGTAA GGGAGCTCCA 
TGTAACTCAG TGACCGGGAA ATGCCACTGT CCTGCAGGGC AGACAGGACA ACACTGTGAA 
AAAGAGTGTG AAGAGGGCCG TTGGGGTCTT GGCTGTGCAG AATCATGTCC TTCCTGTGAC 
AATGGTGGAG TATGTGATAG ACAAAACGGC ACCTGTGTTT GTTCGCCGGG ATTCATGGGA 
AATCTCTGTC AGAATGTCTG TCCAGATGGG CACTTTGGCC TGAGCTGCCT GTTGCGCTGC 
TCCTGTCAAA ACGAAGGGAA ATGCCACCAT GTGACTGGCC TCTGTGTTTG TAAAGACGGC 
TGGATGGGAC CTAACTGCGC AAAAGCTTGT GTGGCTGGAC GATGGGGGCC GAACTGTGCC 
AACCAGTGTG ACTGCAGGAG CAGCGTGGGC AGCTGTGACC CTGTGATTGG CCAGTGTCAT 
TGTGAAGCGG GCTACATGGG ACCACGCTGT GATCAAAAGT GTCCTAAGGG TTTCTTCGGC 
CAGAGCTGCC AGCACAGATG CCAGTGTGAG AATGGCGCAG CGTGTGAGCA TGTGAGCGGT 
GCCTGCACTT GTTTGGCTGG CTGGACTGGG ACGTATTGTG AAAAACCATG TCCTGAGGGC 
TTTCATGGCT TGGAGTGCCA GCAGAAGTGC CAGTGCTTGA ATGGTGGCCG TTGTCATCCC 
GTGACTGGAG GCTGCCAGTG TCCATCTGGT TGGGTCGGGC CAGTTTGCAA TGCCACGTGT 
GAAGCTGGCA CCTTTGGGCC GGCCTGCAGT CAAACCTGTA GATGCCACAA TAATGGCACC 
TGTGACCCTG CTGATGGACG ATGTGAGTGT GGAGCAGGAT GGACAGGCAG CAGGTGTGAA 
CAAGAGTGCT CAGCTGGATT GTATGGTGTG GGCTGCAACC AGCATTGCCT GTGTCAGAAT 
GGAGGGTCGT GTGACCGAAT CAGTGGCCAC TGCACATGTG TCAGTGGGTG GACAGGAGTG 
GCCTGTGAAT TGGAATGTGC TGCGGGACAC TTTGGCATGG ACTGTCAGCA CAAGTGTGAG 
TGTGTAAATG GCGGCTTGTG TGACCGTCAC AGTGGCAGGT GTTTGTGCCC AGCAGGTTGG 
ACAGGCACGC ACTGTGAGAC AGCGTGCACT CAAGGTTTTT ATGGTCTGGG CTGTAAGGAG 
CGATGCCGGT GTGATCATAA CGCCGCATGC CACCACATCA CTGGCACCTG CTTGTGTCCC 
GCTGGCTGGA GAGGGACTCA CTGCGAAAAG GTTTGTTTAC CTGGTTCTTA TGGGAAGCGT 
TGTGCTCATC GATGCCAGTG TCCTCATGGA ACCTCCTGTA ATCATGTGAC TGGACAGTGT 
GGTTGCCCAG CGGGATTGAC TGGTAGTGGC TGTGAGCGAA TGTGCCAGTC AGGTACGTTT 
GGGGTAAACT GTAATCAGGT GTGCCAGTGC TCTGAGCTCA ACCAGCTCTG CCACCCTGTC 
ACTGGTGTCT GTTACTGTGC TCCCGGATAC TATGGCAGCA AGTGTGAAAC AGAATGTGAA 
GCGGGCCGTT ATGGACCCAA CTGCGAGCAG GAATGCCAAT GTCTGAATAA CGGTGTATGT 
AAAAGCAGCA CTGGCACCTG CAGCTGCCCA CCGGGATTCA TCGGACCAAA CTGCAACATC 
ACGTGTCCAT TAGGGCGTTA TGGTCAGGAC TGTTTTGGAG TGTCTCTGTG TGGTGAAGGG 
GCTCGCAGTG ACCCTCTCAC AGGCAGATGT GTGTGCAGGG CTGGTCAGCG TGGACGGGAC 
TGTGGGAAAG GCTGTGCTGA GGGCTGGTTT GGAGAGAATT GTGATCAGCG CTGTAACTGC 
AGCAATGGAG GAGTGTGTGA CGCTCTTACA GGACACTGCA GCTGTACACT GGGCTGGACA 
GGAGACACCT GTGAGGAAGT GTGTCCCAGA GGACGGTATG GTTCAGACTG CAGTGAAGTG 
TGTGACTGTA AGAATAACAG CACATGCGAC CGAGTGACTG GCGTCTGTCT CTGCTCTCCA 
GGTTTCTACG GACATCTATG TCAACATGCA TGTCCTGTTG GCTTCCATGG TCATCGTTGC 
CAGCTTCTGT GTGACTGTGG TGCAAATGCA CCTTGTGACC CTGAAACTGG CCGATGCCTC 
TGTCCTGCAG GATACCAGGG ACTCCGCTGT GAGAGAGAGT GTGAATTGGG CACATTCGGG 
CCTGATTGTG TCCACAGATG TGACTGTGAT GGAGACACCC CATGTGACCC TATTAATGGC 
AGGTGTTTGT GTGCACCTGG AAAAACAGGC TCTCGCTGTG ACATAGGCTG TGGAGTGAAC 
CGATTTGGAC CCGACTGCTC ACAGAGATGT GAATGTCTGA ACGGAGGTCA GTGTGATCCC 
CGAAACGGCC GATGCACGTG TCTGAACGGC TGGATCGGGC CCAGCTGTCA ACAAGGGCTC 
CTGTCTGCTA GCAAAAGTAA AAGGAAGGAC TCGTCAGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329334.1
CDS	109..4650
Translation	MDLHVLLLLLFLLTKIRPSCCLRALHPFMPNVCSEPERVIRDEKQPCVQAFIQMVKVWRQGCTGHAWCMDFERRTTYYIGYKQVYRQDFKMAYKCCPGWSQNNSKMGCLYHIDECQVHNGGCQHRCVNTRGSYYCECNPGFRLHVDGRTCIAVLSCSVNNGGCEHECVQLSPVHFQCRCRRGYQLTADGKRCRLRNPCVEKNGGCVHLCRSDGGRVVCSCHQGFMLAMDQRSCEDVNECETGLAACAHSCRNTPGSFSCICNPGYELGSDGRKCYRIEMEIVNTCDSDNGGCSHRCQHGVLGPVCSCNHGYQLAEDLKTCLDLEECEEGTSCCEQDCTNYPGGYECYCRAGYRLNADGCGCDDVDECASDRGGCEHTCQNTAGSYHCFCRFGYRLGEDRHSCISLQQSVEELPGPVVVERPLLHLALLQDYPQPLERYHDYEEDSFGELRAESTFAEKFVCLNGTFGHDCSLTCDDCSNGGYCNAEGDACDCPDGWTGILCNQTCPEGTFGKNCSFTCKCRNGGTCDPISGKCRCPPGVNGDLCQDGCPKGMYGKHCNKKCNCANNGRCHRTYGACLCDPGLYGRFCHLPCPKWSYGSGCSSECQCVQQNTLECHRRHGTCVCKPGYQGKTCNRECDSGFYGPGCRLKCQCPAGVSCHPMSGQCQRQCPAGLHGEHCDRECPMGQFGVGCTQFCDCKGAPCNSVTGKCHCPAGQTGQHCEKECEEGRWGLGCAESCPSCDNGGVCDRQNGTCVCSPGFMGNLCQNVCPDGHFGLSCLLRCSCQNEGKCHHVTGLCVCKDGWMGPNCAKACVAGRWGPNCANQCDCRSSVGSCDPVIGQCHCEAGYMGPRCDQKCPKGFFGQSCQHRCQCENGAACEHVSGACTCLAGWTGTYCEKPCPEGFHGLECQQKCQCLNGGRCHPVTGGCQCPSGWVGPVCNATCEAGTFGPACSQTCRCHNNGTCDPADGRCECGAGWTGSRCEQECSAGLYGVGCNQHCLCQNGGSCDRISGHCTCVSGWTGVACELECAAGHFGMDCQHKCECVNGGLCDRHSGRCLCPAGWTGTHCETACTQGFYGLGCKERCRCDHNAACHHITGTCLCPAGWRGTHCEKVCLPGSYGKRCAHRCQCPHGTSCNHVTGQCGCPAGLTGSGCERMCQSGTFGVNCNQVCQCSELNQLCHPVTGVCYCAPGYYGSKCETECEAGRYGPNCEQECQCLNNGVCKSSTGTCSCPPGFIGPNCNITCPLGRYGQDCFGVSLCGEGARSDPLTGRCVCRAGQRGRDCGKGCAEGWFGENCDQRCNCSNGGVCDALTGHCSCTLGWTGDTCEEVCPRGRYGSDCSEVCDCKNNSTCDRVTGVCLCSPGFYGHLCQHACPVGFHGHRCQLLCDCGANAPCDPETGRCLCPAGYQGLRCERECELGTFGPDCVHRCDCDGDTPCDPINGRCLCAPGKTGSRCDIGCGVNRFGPDCSQRCECLNGGQCDPRNGRCTCLNGWIGPSCQQGLLSASKSKRKDSSV

Target ORF information:

RefSeq Version	XM_021473659.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473659.1

ORF Insert Sequence:

ATGGATTTAC ACGTGTTGTT GTTATTATTA TTCCTGCTAA CCAAGATCCG TCCATCGTGT 
TGTCTTCGAG CTCTACATCC TTTCATGCCG AATGTTTGCT CAGAGCCAGA GAGAGTCATC 
CGAGATGAGA AACAGCCATG TGTGCAGGCA TTTATACAAA TGGTGAAGGT CTGGAGGCAA 
GGATGCACAG GTCATGCTTG GTGTATGGAC TTCGAGAGGA GGACTACCTA CTACATAGGA 
TATAAACAAG TCTACAGGCA GGACTTCAAG ATGGCATACA AATGCTGTCC TGGATGGTCC 
CAGAACAATT CAAAGATGGG CTGCCTTTAT CATATTGATG AGTGCCAGGT TCACAACGGA 
GGCTGCCAGC ACAGATGTGT GAACACTAGA GGCTCGTACT ACTGCGAGTG CAATCCAGGC 
TTTCGGCTCC ACGTTGATGG CCGCACCTGC ATAGCTGTTT TATCCTGTTC GGTAAATAAT 
GGAGGTTGTG AGCATGAATG TGTCCAGCTT TCTCCAGTTC ACTTCCAGTG CCGCTGCAGA 
CGTGGATACC AGCTTACAGC AGATGGCAAG CGCTGTAGAT TGAGGAACCC ATGTGTGGAG 
AAGAATGGCG GCTGTGTGCA TTTATGTCGC AGTGATGGCG GTCGGGTTGT GTGCAGCTGT 
CACCAAGGCT TCATGCTAGC AATGGACCAG AGGAGCTGTG AAGATGTCAA TGAGTGTGAA 
ACAGGACTGG CAGCATGTGC ACACAGTTGC AGGAACACTC CAGGATCGTT TTCCTGCATC 
TGCAATCCAG GATACGAGCT GGGCTCTGAT GGAAGGAAAT GCTACCGTAT TGAAATGGAG 
ATTGTCAATA CCTGTGACTC TGATAATGGT GGCTGTTCAC ATCGCTGTCA GCATGGTGTT 
CTTGGGCCTG TCTGCAGCTG TAATCATGGT TATCAGCTCG CCGAAGACCT CAAAACCTGT 
TTGGATCTGG AGGAATGTGA GGAAGGAACG TCATGCTGTG AGCAGGATTG CACAAACTAC 
CCTGGAGGGT ATGAATGCTA CTGCAGGGCA GGTTACAGAC TCAATGCAGA TGGATGCGGA 
TGTGACGATG TGGACGAGTG TGCATCTGAT AGAGGAGGCT GTGAACACAC TTGTCAGAAT 
ACGGCTGGCT CATATCACTG CTTCTGTCGG TTTGGATATC GACTTGGTGA GGACAGACAC 
TCCTGCATTT CTCTCCAGCA ATCGGTTGAG GAATTGCCCG GTCCAGTGGT GGTTGAGCGT 
CCTCTCCTTC ATCTCGCTCT CCTCCAGGAT TACCCACAAC CCCTGGAGCG CTATCATGAC 
TATGAGGAGG ACAGTTTTGG AGAGCTGCGG GCAGAGAGCA CCTTCGCTGA GAAGTTTGTG 
TGTTTGAATG GGACGTTTGG CCATGACTGC AGCCTGACAT GTGATGATTG CTCAAATGGA 
GGATATTGTA ATGCTGAAGG AGATGCTTGT GACTGTCCAG ATGGGTGGAC GGGGATCCTC 
TGTAACCAAA CCTGTCCAGA GGGAACATTT GGTAAAAACT GCTCCTTCAC TTGCAAGTGC 
AGAAACGGGG GCACCTGTGA CCCGATTAGT GGGAAGTGCC GCTGCCCACC TGGAGTCAAT 
GGAGACCTGT GCCAGGATGG CTGTCCGAAG GGAATGTATG GAAAGCACTG CAACAAGAAA 
TGTAACTGTG CTAATAACGG CCGCTGCCAT CGCACATATG GAGCCTGTCT GTGTGATCCA 
GGACTCTACG GCAGGTTCTG TCACCTCCCT TGCCCCAAGT GGTCGTATGG ATCTGGCTGC 
TCATCCGAGT GTCAGTGTGT GCAACAAAAC ACGCTTGAGT GCCATCGACG TCATGGAACG 
TGTGTGTGCA AACCTGGCTA TCAGGGGAAA ACCTGCAACC GCGAGTGTGA TAGTGGATTT 
TATGGTCCAG GATGTAGATT AAAATGCCAG TGTCCAGCGG GAGTCTCTTG CCATCCCATG 
AGTGGGCAAT GTCAGCGTCA GTGCCCAGCA GGCCTACATG GGGAGCACTG TGACCGAGAG 
TGCCCCATGG GACAGTTTGG AGTTGGATGC ACACAGTTTT GTGACTGTAA GGGAGCTCCA 
TGTAACTCAG TGACCGGGAA ATGCCACTGT CCTGCAGGGC AGACAGGACA ACACTGTGAA 
AAAGAGTGTG AAGAGGGCCG TTGGGGTCTT GGCTGTGCAG AATCATGTCC TTCCTGTGAC 
AATGGTGGAG TATGTGATAG ACAAAACGGC ACCTGTGTTT GTTCGCCGGG ATTCATGGGA 
AATCTCTGTC AGAATGTCTG TCCAGATGGG CACTTTGGCC TGAGCTGCCT GTTGCGCTGC 
TCCTGTCAAA ACGAAGGGAA ATGCCACCAT GTGACTGGCC TCTGTGTTTG TAAAGACGGC 
TGGATGGGAC CTAACTGCGC AAAAGCTTGT GTGGCTGGAC GATGGGGGCC GAACTGTGCC 
AACCAGTGTG ACTGCAGGAG CAGCGTGGGC AGCTGTGACC CTGTGATTGG CCAGTGTCAT 
TGTGAAGCGG GCTACATGGG ACCACGCTGT GATCAAAAGT GTCCTAAGGG TTTCTTCGGC 
CAGAGCTGCC AGCACAGATG CCAGTGTGAG AATGGCGCAG CGTGTGAGCA TGTGAGCGGT 
GCCTGCACTT GTTTGGCTGG CTGGACTGGG ACGTATTGTG AAAAACCATG TCCTGAGGGC 
TTTCATGGCT TGGAGTGCCA GCAGAAGTGC CAGTGCTTGA ATGGTGGCCG TTGTCATCCC 
GTGACTGGAG GCTGCCAGTG TCCATCTGGT TGGGTCGGGC CAGTTTGCAA TGCCACGTGT 
GAAGCTGGCA CCTTTGGGCC GGCCTGCAGT CAAACCTGTA GATGCCACAA TAATGGCACC 
TGTGACCCTG CTGATGGACG ATGTGAGTGT GGAGCAGGAT GGACAGGCAG CAGGTGTGAA 
CAAGAGTGCT CAGCTGGATT GTATGGTGTG GGCTGCAACC AGCATTGCCT GTGTCAGAAT 
GGAGGGTCGT GTGACCGAAT CAGTGGCCAC TGCACATGTG TCAGTGGGTG GACAGGAGTG 
GCCTGTGAAT TGGAATGTGC TGCGGGACAC TTTGGCATGG ACTGTCAGCA CAAGTGTGAG 
TGTGTAAATG GCGGCTTGTG TGACCGTCAC AGTGGCAGGT GTTTGTGCCC AGCAGGTTGG 
ACAGGCACGC ACTGTGAGAC AGCGTGCACT CAAGGTTTTT ATGGTCTGGG CTGTAAGGAG 
CGATGCCGGT GTGATCATAA CGCCGCATGC CACCACATCA CTGGCACCTG CTTGTGTCCC 
GCTGGCTGGA GAGGGACTCA CTGCGAAAAG GTTTGTTTAC CTGGTTCTTA TGGGAAGCGT 
TGTGCTCATC GATGCCAGTG TCCTCATGGA ACCTCCTGTA ATCATGTGAC TGGACAGTGT 
GGTTGCCCAG CGGGATTGAC TGGTAGTGGC TGTGAGCGAA TGTGCCAGTC AGGTACGTTT 
GGGGTAAACT GTAATCAGGT GTGCCAGTGC TCTGAGCTCA ACCAGCTCTG CCACCCTGTC 
ACTGGTGTCT GTTACTGTGC TCCCGGATAC TATGGCAGCA AGTGTGAAAC AGAATGTGAA 
GCGGGCCGTT ATGGACCCAA CTGCGAGCAG GAATGCCAAT GTCTGAATAA CGGTGTATGT 
AAAAGCAGCA CTGGCACCTG CAGCTGCCCA CCGGGATTCA TCGGACCAAA CTGCAACATC 
ACGTGTCCAT TAGGGCGTTA TGGTCAGGAC TGTTTTGGAG TGTCTCTGTG TGGTGAAGGG 
GCTCGCAGTG ACCCTCTCAC AGGCAGATGT GTGTGCAGGG CTGGTCAGCG TGGACGGGAC 
TGTGGGAAAG GCTGTGCTGA GGGCTGGTTT GGAGAGAATT GTGATCAGCG CTGTAACTGC 
AGCAATGGAG GAGTGTGTGA CGCTCTTACA GGACACTGCA GCTGTACACT GGGCTGGACA 
GGAGACACCT GTGAGGAAGT GTGTCCCAGA GGACGGTATG GTTCAGACTG CAGTGAAGTG 
TGTGACTGTA AGAATAACAG CACATGCGAC CGAGTGACTG GCGTCTGTCT CTGCTCTCCA 
GGTTTCTACG GACATCTATG TCAACATGCA TGTCCTGTTG GCTTCCATGG TCATCGTTGC 
CAGCTTCTGT GTGACTGTGG TGCAAATGCA CCTTGTGACC CTGAAACTGG CCGATGCCTC 
TGTCCTGCAG GATACCAGGG ACTCCGCTGT GAGAGAGAGT GTGAATTGGG CACATTCGGG 
CCTGATTGTG TCCACAGATG TGACTGTGAT GGAGACACCC CATGTGACCC TATTAATGGC 
AGGTGTTTGT GTGCACCTGG AAAAACAGGC TCTCGCTGTG ACATAGGCTG TGGAGTGAAC 
CGATTTGGAC CCGACTGCTC ACAGAGATGT GAATGTCTGA ACGGAGGTCA GTGTGATCCC 
CGAAACGGCC GATGCACGTG TCTGAACGGC TGGATCGGGC CCAGCTGTCA ACAAGGGCTC 
CTGTCTGCTA GCAAAAGTAA AAGGAAGGAC TCGTCAGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

megf6a ( XM_017353739.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209228.2 Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X1, mRNA.

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CloneID	ODa151555
Clone ID Related Accession (Same CDS sequence)	XM_017353739.2
Accession Version	XM_017353739.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	multiple epidermal growth factor-like domains protein 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353739.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAC ACGTGTTGTT GTTATTATTA TTCCTGCTAA CCAAGATCCG TCCATCGTGT 
TGTCTTCGAG CTCTACATCC TTTCATGCCG AATGTTTGCT CAGAGCCAGA GAGAGTCATC 
CGAGCTGAGA AACAGCCATG TGTGCAGGCA TTTATACAAA TGGTGAAGGT CTGGAGGCAA 
GGATGCACAG GTCATGCTTG GTGTATGGAC TTCGAGAGGA GGACTACCTA CTACATAGGA 
TATAAACAAG TCTACAGGCA GGACTTCAAG ATGGCATACA AATGCTGTCC TGGATGGTCC 
CAGAACAATT CAAAGATGGG CTGCCTTTAT CATATTGATG AGTGCCAGGT TCACAACGGA 
GGCTGCCAGC ACAGATGTGT GAACACTAGA GGCTCGTACT ACTGCGAGTG CAATCCAGGC 
TTTCGGCTCC ACGTTGATGG CCGCACCTGC ATAGCTGTTT TATCCTGTTC GGTAAATAAT 
GGAGGTTGTG AGCATGAATG TGTCCAGCTT TCTCCAGTTC ACTTCCAGTG CCGCTGCAGA 
CGTGGATACC AGCTTACAGC AGATGGCAAG CGCTGTAGAT TGAGGAACCC ATGTGTGGAG 
AAGAATGGCG GCTGTGTGCA TTTATGTCGC AGTGATGGTG GTCGGGTTGT GTGCAGCTGT 
CACCAAGGCT TCATGCTAGC AGTGGACCAG AAGAGCTGTG AAGATGTCAA TGAGTGTGAA 
ACAGGACTGG CAGCATGTGC ACACAGTTGC AGGAACACTC CAGGATCGTT TTCCTGCATC 
TGCAATCCAG GATACGAGCT GGGCTCTGAT GGAAGGAAAT GCTACCGTAT TGAAATGGAG 
ATTGTCAATA CCTGTGACTC TGATAATGGT GGCTGTTCAC ATCGCTGTCA GCATGGTGTT 
CTTGGGCCTG TCTGCAGCTG TAATCATGGT TATCAGCTCG CCGAAGACCT CAAAACCTGT 
TTGGATCTGG AGGAATGTGA GGAAGGAACG TCATGCTGTG AGCAGGATTG CACAAACTAC 
CCTGGAGGGT ATGAATGCTA CTGCAGGGCA GGTTACAGAC TCAATGCAGA TGGATGCGGA 
TGTGACGATG TGGACGAGTG TGCGTCTGAT AGAGGAGGCT GTGAACACAC TTGTCAGAAT 
ACGGCTGGCT CATATCACTG CTTCTGTCGG TTTGGATATC GACTTGGTGA GGACAGACAC 
TCCTGCATTT CTCTCCAGCG ATCGGTTGAG GAATTGCCCG GTCCAGTGGT GGTTGAGCGT 
CCTCTCCTTC ATCTCGCTCT CCTCCAGGAT TACCCACAAC CCCTGGAGCG CTATCATGAC 
TATGAGGAGG ACAGTTTTGG AGAGCTGCGG GCAGAGAGCA CCTTCGCTGA GAAGTTTGTG 
TGTTTGAATG GGACGTTTGG CCATGACTGC AGCCTGACGT GTGATGATTG CTCAAATGGA 
GGATATTGTA ATGCTGAAGG AGATGCTTGT GACTGTCCAG ATGGGTGGAC GGGGATCCTT 
TGTAACCAAA CCTGTCCAGA GGGAACATTT GGTAAAAACT GCTCCTTCAC TTGCAAGTGC 
AGAAACGGGG GCACCTGTGA CCCGATTAGT GGGAAGTGCC GCTGCCCACC TGGAGTCAAT 
GGAGACCTGT GCCAGGACGG CTGTCCGAAG GGAATGTATG GAAAGCACTG CAACAAGAAA 
TGTAACTGTG CTAATAACGG CCGCTGCCAT CGCACATATG GAGCCTGTCT GTGTGATCCA 
GGACTCTACG GCAGGTTCTG TCACCTCCCT TGCCCCAAGT GGTCGTATGG ATCTGGCTGC 
TCATCCGAGT GTCAGTGTGT GCAACAAAAC ACGCTTGAGT GCCATCGACG TCATGGAACG 
TGTGTGTGCA AACCTGGCTA TCAGGGGAAA ACCTGCAACC GCGAGTGTGA TAGTGGATTT 
TATGGTCCAG GATGTAGATT AAAATGCCAG TGTCCAGCGG GAGTCTCTTG CCATCCCATG 
AGTGGGCAAT GTCAGCGTCA GTGCCCAGCA GGCCTACATG GGGAGCACTG TGACCGAGAG 
TGCCCCATGG GACAGTTTGG AGTTGGATGC ACACAGTTTT GTGACTGTAA GGGAGCTCCA 
TGTAACTCAG TGACCGGGAA ATGCCACTGT CCTGCAGGGC AGACAGGACA ACACTGTGAA 
AAAGAGTGTG AAGAGGGCCG TTGGGGTCTT GGCTGTGCAG AATCATGTCC TTCCTGTGAC 
AATGGTGGAG TATGTGATAG ACAAAACGGC ACCTGTGTTT GTTCGCCGGG ATTCATGGGA 
AATCTCTGTC AGAATGTCTG TCCAGATGGG CACTTTGGCC TGAGCTGCCT GTTGCGCTGC 
TCCTGTCAAA ACGAAGGGAA ATGCCACCAT GTGACTGGCC TCTGTGTTTG TAAAGATGGC 
TGGATGGGAC CCAACTGCGC AAAAGCTTGT GTGGCTGGAC GATGGGGGCC GAACTGTGCC 
AACCAGTGTG ACTGCAGGAG CAGCGTGGGC AGCTGTGACC CTGTGATTGG CCTGTGTCAT 
TGTGAAGCGG GCTACATGGG ACCACGCTGT GATCAAAAGT GTCCTCAGGG TTTCTTCGGC 
CAGAGCTGCC AGCACAGATG CCAGTGTGAG AATGGCGCAG TGTGTGAGCA TGTGAGCGGT 
GCCTGCACTT GTTTGGCTGG CTGGACTGGG ACGTATTGTG AAAAACCTTG TCCTGAGGGC 
TTTCATGGCT TGGAGTGCCA GCAGAAGTGC CAGTGCTTGA ATGGTGGCCG TTGTCATCCC 
GTGACTGGAG GCTGCCAGTG TCCATCTGGT TGGGTCGGGC CAGTTTGCAA TGCCACGTGT 
GAAGCTGGCA CCTTTGGGCC GGCCTGCAGT CAAACCTGTA GATGCCACAA TAATGGCACC 
TGTGACCCTG CTGATGGGCG ATGTGAGTGT GGAGCAGGAT GGACAGGCAG CAGCTGTGAA 
CAAGAGTGCT CAGCTGGATT GTATGGTGTG GGCTGCAACC AGCATTGCCT GTGTCAGAAT 
GGAGGGTCGT GTGACCGAAT CAGTGGCCAC TGCACATGTG TCAGTGGGTG GACAGGAGTG 
GCCTGTGAAT TGGAATGTGC TGCGGGACAC TTTGGCATGG ACTGTCAGCA CAAGTGTGAG 
TGTGTAAATG GCGGCGTGTG TGACCGTCAC AGTGGCAGGT GTTTGTGCCC AGCAGGTTGG 
ACAGGCACGC ACTGTGAGAC AGCATGCACT CAAGGTTTTT ATGGTCCGGG CTGTAAGGAG 
CGATGCCGGT GTGATCATAA CGCCGCATGC CACCACATCA CTGGCACCTG CTTGTGTCCC 
GCTGGCTGGA GAGGGACTCA CTGCGAAAAG GTTTGTTTAC CTGGTTCCTA TGGGAAGCGT 
TGTGCTCATC GATGCCAGTG TCCTCATGGA ACCTCCTGTA ACCATGTGAC TGGACAGTGT 
GGTTGCCCAG CGGGATTAAC TGGTAGTGGC TGTGAGCGAA TGTGCCAGTC AGGTACGTTT 
GGGGTAAACT GTAATCAGGT GTGCCAGTGC TCTGAGCTCA ACCAGCTCTG CCACCCCGTC 
ACTGGTGTCT GTTACTGTGC TCCCGGATAC TATGGCAGCA AGTGTGAAAC AGAATGTGAA 
GCGGGCCGTT ATGGACCCAA CTGTGAGCAG GAATGCCAGT GTCTGAATAA CGGTGTATGT 
AAAAGCAGCA CTGGCACCTG CAGCTGCCCA CCGGGATTCA TCGGACCAAA CTGCAACATC 
ACATGTCCAT TAGGGCGTTA TGGTCAGGAC TGTGCTGGAG TGTCTCTGTG TGGTGAAGGG 
GCTCGCAGTG ACCCTCTCAC AGGCAGATGT GTGTGCAGGG CTGGTCAGCG TGGACGGGAC 
TGTGGGAAAG GCTGTGCTGA GGGCTGGTTT GGAGAGAATT GTGATCAGCG CTGTAACTGC 
AGCAATGGCG GAGTGTGTGA CGCTCTTACA GGACACTGCA GCTGTACACT GGGCTGGACA 
GGAGACACCT GTGAGGAAGT GTGTCCCAGA GGACGGTATG GTTCAGACTG CAGTGAAGTG 
TGTGACTGTA AGAATAACAG CACATGCGAC CGAGTGACTG GCGTCTGTCT CTGCTCTCCA 
GGTTTCTACG GACATCTATG TCAACATGCA TGTCCTGTTG GCTTCCATGG TCATCGTTGC 
CAGCTTCTGT GTGACTGTGG TGCAAATGCA CCTTGTGACC CTGAAACTGG CCGATGCCTC 
TGTCCTGCAG GATACCAGGG ACTCCGCTGT GAGAGAGAGT GTGAATTGGG CACATTTGGG 
GCTGATTGTG TCCACAGATG TGACTGTGAT GGAGACACCC CATGTGACCC TATTAATGGC 
AGGTGTTTGT GTGCACCTGG AAAAACAGGC TCTCGCTGTG ACATAGGCTG TGGAGTGAAC 
CGATTTGGAC CCGACTGCTC ACAGAGATGT GAATGTCTGA ACGGAGGTCA GTGTGATCCC 
CGAAACGGCC GATGCACGTG TCTGAACGGC TGGATCGGGC CCAGCTGTCA ACAAGGGCTC 
CTGTCTGCTA GCAAAAGTAA AAGGAAGGAC TCGTCAGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209228.2
CDS	111..4652
Translation	MDLHVLLLLLFLLTKIRPSCCLRALHPFMPNVCSEPERVIRAEKQPCVQAFIQMVKVWRQGCTGHAWCMDFERRTTYYIGYKQVYRQDFKMAYKCCPGWSQNNSKMGCLYHIDECQVHNGGCQHRCVNTRGSYYCECNPGFRLHVDGRTCIAVLSCSVNNGGCEHECVQLSPVHFQCRCRRGYQLTADGKRCRLRNPCVEKNGGCVHLCRSDGGRVVCSCHQGFMLAVDQKSCEDVNECETGLAACAHSCRNTPGSFSCICNPGYELGSDGRKCYRIEMEIVNTCDSDNGGCSHRCQHGVLGPVCSCNHGYQLAEDLKTCLDLEECEEGTSCCEQDCTNYPGGYECYCRAGYRLNADGCGCDDVDECASDRGGCEHTCQNTAGSYHCFCRFGYRLGEDRHSCISLQRSVEELPGPVVVERPLLHLALLQDYPQPLERYHDYEEDSFGELRAESTFAEKFVCLNGTFGHDCSLTCDDCSNGGYCNAEGDACDCPDGWTGILCNQTCPEGTFGKNCSFTCKCRNGGTCDPISGKCRCPPGVNGDLCQDGCPKGMYGKHCNKKCNCANNGRCHRTYGACLCDPGLYGRFCHLPCPKWSYGSGCSSECQCVQQNTLECHRRHGTCVCKPGYQGKTCNRECDSGFYGPGCRLKCQCPAGVSCHPMSGQCQRQCPAGLHGEHCDRECPMGQFGVGCTQFCDCKGAPCNSVTGKCHCPAGQTGQHCEKECEEGRWGLGCAESCPSCDNGGVCDRQNGTCVCSPGFMGNLCQNVCPDGHFGLSCLLRCSCQNEGKCHHVTGLCVCKDGWMGPNCAKACVAGRWGPNCANQCDCRSSVGSCDPVIGLCHCEAGYMGPRCDQKCPQGFFGQSCQHRCQCENGAVCEHVSGACTCLAGWTGTYCEKPCPEGFHGLECQQKCQCLNGGRCHPVTGGCQCPSGWVGPVCNATCEAGTFGPACSQTCRCHNNGTCDPADGRCECGAGWTGSSCEQECSAGLYGVGCNQHCLCQNGGSCDRISGHCTCVSGWTGVACELECAAGHFGMDCQHKCECVNGGVCDRHSGRCLCPAGWTGTHCETACTQGFYGPGCKERCRCDHNAACHHITGTCLCPAGWRGTHCEKVCLPGSYGKRCAHRCQCPHGTSCNHVTGQCGCPAGLTGSGCERMCQSGTFGVNCNQVCQCSELNQLCHPVTGVCYCAPGYYGSKCETECEAGRYGPNCEQECQCLNNGVCKSSTGTCSCPPGFIGPNCNITCPLGRYGQDCAGVSLCGEGARSDPLTGRCVCRAGQRGRDCGKGCAEGWFGENCDQRCNCSNGGVCDALTGHCSCTLGWTGDTCEEVCPRGRYGSDCSEVCDCKNNSTCDRVTGVCLCSPGFYGHLCQHACPVGFHGHRCQLLCDCGANAPCDPETGRCLCPAGYQGLRCERECELGTFGADCVHRCDCDGDTPCDPINGRCLCAPGKTGSRCDIGCGVNRFGPDCSQRCECLNGGQCDPRNGRCTCLNGWIGPSCQQGLLSASKSKRKDSSV

Target ORF information:

RefSeq Version	XM_017353739.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353739.2

ORF Insert Sequence:

ATGGATTTAC ACGTGTTGTT GTTATTATTA TTCCTGCTAA CCAAGATCCG TCCATCGTGT 
TGTCTTCGAG CTCTACATCC TTTCATGCCG AATGTTTGCT CAGAGCCAGA GAGAGTCATC 
CGAGCTGAGA AACAGCCATG TGTGCAGGCA TTTATACAAA TGGTGAAGGT CTGGAGGCAA 
GGATGCACAG GTCATGCTTG GTGTATGGAC TTCGAGAGGA GGACTACCTA CTACATAGGA 
TATAAACAAG TCTACAGGCA GGACTTCAAG ATGGCATACA AATGCTGTCC TGGATGGTCC 
CAGAACAATT CAAAGATGGG CTGCCTTTAT CATATTGATG AGTGCCAGGT TCACAACGGA 
GGCTGCCAGC ACAGATGTGT GAACACTAGA GGCTCGTACT ACTGCGAGTG CAATCCAGGC 
TTTCGGCTCC ACGTTGATGG CCGCACCTGC ATAGCTGTTT TATCCTGTTC GGTAAATAAT 
GGAGGTTGTG AGCATGAATG TGTCCAGCTT TCTCCAGTTC ACTTCCAGTG CCGCTGCAGA 
CGTGGATACC AGCTTACAGC AGATGGCAAG CGCTGTAGAT TGAGGAACCC ATGTGTGGAG 
AAGAATGGCG GCTGTGTGCA TTTATGTCGC AGTGATGGTG GTCGGGTTGT GTGCAGCTGT 
CACCAAGGCT TCATGCTAGC AGTGGACCAG AAGAGCTGTG AAGATGTCAA TGAGTGTGAA 
ACAGGACTGG CAGCATGTGC ACACAGTTGC AGGAACACTC CAGGATCGTT TTCCTGCATC 
TGCAATCCAG GATACGAGCT GGGCTCTGAT GGAAGGAAAT GCTACCGTAT TGAAATGGAG 
ATTGTCAATA CCTGTGACTC TGATAATGGT GGCTGTTCAC ATCGCTGTCA GCATGGTGTT 
CTTGGGCCTG TCTGCAGCTG TAATCATGGT TATCAGCTCG CCGAAGACCT CAAAACCTGT 
TTGGATCTGG AGGAATGTGA GGAAGGAACG TCATGCTGTG AGCAGGATTG CACAAACTAC 
CCTGGAGGGT ATGAATGCTA CTGCAGGGCA GGTTACAGAC TCAATGCAGA TGGATGCGGA 
TGTGACGATG TGGACGAGTG TGCGTCTGAT AGAGGAGGCT GTGAACACAC TTGTCAGAAT 
ACGGCTGGCT CATATCACTG CTTCTGTCGG TTTGGATATC GACTTGGTGA GGACAGACAC 
TCCTGCATTT CTCTCCAGCG ATCGGTTGAG GAATTGCCCG GTCCAGTGGT GGTTGAGCGT 
CCTCTCCTTC ATCTCGCTCT CCTCCAGGAT TACCCACAAC CCCTGGAGCG CTATCATGAC 
TATGAGGAGG ACAGTTTTGG AGAGCTGCGG GCAGAGAGCA CCTTCGCTGA GAAGTTTGTG 
TGTTTGAATG GGACGTTTGG CCATGACTGC AGCCTGACGT GTGATGATTG CTCAAATGGA 
GGATATTGTA ATGCTGAAGG AGATGCTTGT GACTGTCCAG ATGGGTGGAC GGGGATCCTT 
TGTAACCAAA CCTGTCCAGA GGGAACATTT GGTAAAAACT GCTCCTTCAC TTGCAAGTGC 
AGAAACGGGG GCACCTGTGA CCCGATTAGT GGGAAGTGCC GCTGCCCACC TGGAGTCAAT 
GGAGACCTGT GCCAGGACGG CTGTCCGAAG GGAATGTATG GAAAGCACTG CAACAAGAAA 
TGTAACTGTG CTAATAACGG CCGCTGCCAT CGCACATATG GAGCCTGTCT GTGTGATCCA 
GGACTCTACG GCAGGTTCTG TCACCTCCCT TGCCCCAAGT GGTCGTATGG ATCTGGCTGC 
TCATCCGAGT GTCAGTGTGT GCAACAAAAC ACGCTTGAGT GCCATCGACG TCATGGAACG 
TGTGTGTGCA AACCTGGCTA TCAGGGGAAA ACCTGCAACC GCGAGTGTGA TAGTGGATTT 
TATGGTCCAG GATGTAGATT AAAATGCCAG TGTCCAGCGG GAGTCTCTTG CCATCCCATG 
AGTGGGCAAT GTCAGCGTCA GTGCCCAGCA GGCCTACATG GGGAGCACTG TGACCGAGAG 
TGCCCCATGG GACAGTTTGG AGTTGGATGC ACACAGTTTT GTGACTGTAA GGGAGCTCCA 
TGTAACTCAG TGACCGGGAA ATGCCACTGT CCTGCAGGGC AGACAGGACA ACACTGTGAA 
AAAGAGTGTG AAGAGGGCCG TTGGGGTCTT GGCTGTGCAG AATCATGTCC TTCCTGTGAC 
AATGGTGGAG TATGTGATAG ACAAAACGGC ACCTGTGTTT GTTCGCCGGG ATTCATGGGA 
AATCTCTGTC AGAATGTCTG TCCAGATGGG CACTTTGGCC TGAGCTGCCT GTTGCGCTGC 
TCCTGTCAAA ACGAAGGGAA ATGCCACCAT GTGACTGGCC TCTGTGTTTG TAAAGATGGC 
TGGATGGGAC CCAACTGCGC AAAAGCTTGT GTGGCTGGAC GATGGGGGCC GAACTGTGCC 
AACCAGTGTG ACTGCAGGAG CAGCGTGGGC AGCTGTGACC CTGTGATTGG CCTGTGTCAT 
TGTGAAGCGG GCTACATGGG ACCACGCTGT GATCAAAAGT GTCCTCAGGG TTTCTTCGGC 
CAGAGCTGCC AGCACAGATG CCAGTGTGAG AATGGCGCAG TGTGTGAGCA TGTGAGCGGT 
GCCTGCACTT GTTTGGCTGG CTGGACTGGG ACGTATTGTG AAAAACCTTG TCCTGAGGGC 
TTTCATGGCT TGGAGTGCCA GCAGAAGTGC CAGTGCTTGA ATGGTGGCCG TTGTCATCCC 
GTGACTGGAG GCTGCCAGTG TCCATCTGGT TGGGTCGGGC CAGTTTGCAA TGCCACGTGT 
GAAGCTGGCA CCTTTGGGCC GGCCTGCAGT CAAACCTGTA GATGCCACAA TAATGGCACC 
TGTGACCCTG CTGATGGGCG ATGTGAGTGT GGAGCAGGAT GGACAGGCAG CAGCTGTGAA 
CAAGAGTGCT CAGCTGGATT GTATGGTGTG GGCTGCAACC AGCATTGCCT GTGTCAGAAT 
GGAGGGTCGT GTGACCGAAT CAGTGGCCAC TGCACATGTG TCAGTGGGTG GACAGGAGTG 
GCCTGTGAAT TGGAATGTGC TGCGGGACAC TTTGGCATGG ACTGTCAGCA CAAGTGTGAG 
TGTGTAAATG GCGGCGTGTG TGACCGTCAC AGTGGCAGGT GTTTGTGCCC AGCAGGTTGG 
ACAGGCACGC ACTGTGAGAC AGCATGCACT CAAGGTTTTT ATGGTCCGGG CTGTAAGGAG 
CGATGCCGGT GTGATCATAA CGCCGCATGC CACCACATCA CTGGCACCTG CTTGTGTCCC 
GCTGGCTGGA GAGGGACTCA CTGCGAAAAG GTTTGTTTAC CTGGTTCCTA TGGGAAGCGT 
TGTGCTCATC GATGCCAGTG TCCTCATGGA ACCTCCTGTA ACCATGTGAC TGGACAGTGT 
GGTTGCCCAG CGGGATTAAC TGGTAGTGGC TGTGAGCGAA TGTGCCAGTC AGGTACGTTT 
GGGGTAAACT GTAATCAGGT GTGCCAGTGC TCTGAGCTCA ACCAGCTCTG CCACCCCGTC 
ACTGGTGTCT GTTACTGTGC TCCCGGATAC TATGGCAGCA AGTGTGAAAC AGAATGTGAA 
GCGGGCCGTT ATGGACCCAA CTGTGAGCAG GAATGCCAGT GTCTGAATAA CGGTGTATGT 
AAAAGCAGCA CTGGCACCTG CAGCTGCCCA CCGGGATTCA TCGGACCAAA CTGCAACATC 
ACATGTCCAT TAGGGCGTTA TGGTCAGGAC TGTGCTGGAG TGTCTCTGTG TGGTGAAGGG 
GCTCGCAGTG ACCCTCTCAC AGGCAGATGT GTGTGCAGGG CTGGTCAGCG TGGACGGGAC 
TGTGGGAAAG GCTGTGCTGA GGGCTGGTTT GGAGAGAATT GTGATCAGCG CTGTAACTGC 
AGCAATGGCG GAGTGTGTGA CGCTCTTACA GGACACTGCA GCTGTACACT GGGCTGGACA 
GGAGACACCT GTGAGGAAGT GTGTCCCAGA GGACGGTATG GTTCAGACTG CAGTGAAGTG 
TGTGACTGTA AGAATAACAG CACATGCGAC CGAGTGACTG GCGTCTGTCT CTGCTCTCCA 
GGTTTCTACG GACATCTATG TCAACATGCA TGTCCTGTTG GCTTCCATGG TCATCGTTGC 
CAGCTTCTGT GTGACTGTGG TGCAAATGCA CCTTGTGACC CTGAAACTGG CCGATGCCTC 
TGTCCTGCAG GATACCAGGG ACTCCGCTGT GAGAGAGAGT GTGAATTGGG CACATTTGGG 
GCTGATTGTG TCCACAGATG TGACTGTGAT GGAGACACCC CATGTGACCC TATTAATGGC 
AGGTGTTTGT GTGCACCTGG AAAAACAGGC TCTCGCTGTG ACATAGGCTG TGGAGTGAAC 
CGATTTGGAC CCGACTGCTC ACAGAGATGT GAATGTCTGA ACGGAGGTCA GTGTGATCCC 
CGAAACGGCC GATGCACGTG TCTGAACGGC TGGATCGGGC CCAGCTGTCA ACAAGGGCTC 
CTGTCTGCTA GCAAAAGTAA AAGGAAGGAC TCGTCAGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

megf6a ( XM_009296882.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295157.1 Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X2, mRNA.

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CloneID	ODa44516
Clone ID Related Accession (Same CDS sequence)	XM_009296882.2 , XM_009296882.3
Accession Version	XM_009296882.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	multiple epidermal growth factor-like domains protein 6 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCCG ATTGGTTGAG GTTGGTGCTT GTGTTTACCT TCTGGACTCA GCTGGTACCA 
AGTTTGGCTG CTGGAAATCC ACATTACCGC TACTATCACA GAAACTGCTA CACCTGCTTC 
AATGGATATC ATGCGTACAA CACAGGACAC CAGGGCGCAG ATATTGATGA GTGCCAGGTT 
CACAACGGAG GCTGCCAGCA CAGATGTGTG AACACTAGAG GCTCGTACTA CTGCGAGTGC 
AATCCAGGCT TTCGGCTCCA CGTTGATGGC CGCACCTGCA TAGCTGTTTT ATCCTGTTCG 
GTAAATAATG GAGGTTGTGA GCATGAATGT GTCCAGCTTT CTCCAGTTCA CTTCCAGTGC 
CGCTGCAGAC GTGGATACCA GCTTACAGCA GATGGCAAGC GCTGTAGATT GAGGAACCCA 
TGTGTGGAGA AGAATGGCGG CTGTGTGCAT TTATGTCGCA GTGATGGTGG TCGGGTTGTG 
TGCAGCTGTC ACCAAGGCTT CATGCTAGCA GTGGACCAGA AGAGCTGTGA AGATGTCAAT 
GAGTGTGAAA CAGGACTGGC AGCATGTGCA CACAGTTGCA GGAACACTCC AGGATCGTTT 
TCCTGCATCT GCAATCCAGG ATACGAGCTG GGCTCTGATG GAAGGAAATG CTACCGTATT 
GAAATGGAGA TTGTCAATAC CTGTGACTCT GATAATGGTG GCTGTTCACA TCGCTGTCAG 
CATGGTGTTC TTGGGCCTGT CTGCAGCTGT AATCATGGTT ATCAGCTCGC CGAAGACCTC 
AAAACCTGTT TGGATCTGGA GGAATGTGAG GAAGGAACGT CATGCTGTGA GCAGGATTGC 
ACAAACTACC CTGGAGGGTA TGAATGCTAC TGCAGGGCAG GTTACAGACT CAATGCAGAT 
GGATGCGGAT GTGACGATGT GGACGAGTGT GCGTCTGATA GAGGAGGCTG TGAACACACT 
TGTCAGAATA CGGCTGGCTC ATATCACTGC TTCTGTCGGT TTGGATATCG ACTTGGTGAG 
GACAGACACT CCTGCATTTC TCTCCAGCGA TCGGTTGAGG AATTGCCCGG TCCAGTGGTG 
GTTGAGCGTC CTCTCCTTCA TCTCGCTCTC CTCCAGGATT ACCCACAACC CCTGGAGCGC 
TATCATGACT ATGAGGAGGA CAGTTTTGGA GAGCTGCGGG CAGAGAGCAC CTTCGCTGAG 
AAGTTTGTGT GTTTGAATGG GACGTTTGGC CATGACTGCA GCCTGACGTG TGATGATTGC 
TCAAATGGAG GATATTGTAA TGCTGAAGGA GATGCTTGTG ACTGTCCAGA TGGGTGGACG 
GGGATCCTTT GTAACCAAAC CTGTCCAGAG GGAACATTTG GTAAAAACTG CTCCTTCACT 
TGCAAGTGCA GAAACGGGGG CACCTGTGAC CCGATTAGTG GGAAGTGCCG CTGCCCACCT 
GGAGTCAATG GAGACCTGTG CCAGGACGGC TGTCCGAAGG GAATGTATGG AAAGCACTGC 
AACAAGAAAT GTAACTGTGC TAATAACGGC CGCTGCCATC GCACATATGG AGCCTGTCTG 
TGTGATCCAG GACTCTACGG CAGGTTCTGT CACCTCCCTT GCCCCAAGTG GTCGTATGGA 
TCTGGCTGCT CATCCGAGTG TCAGTGTGTG CAACAAAACA CGCTTGAGTG CCATCGACGT 
CATGGAACGT GTGTGTGCAA ACCTGGCTAT CAGGGGAAAA CCTGCAACCG CGAGTGTGAT 
AGTGGATTTT ATGGTCCAGG ATGTAGATTA AAATGCCAGT GTCCAGCGGG AGTCTCTTGC 
CATCCCATGA GTGGGCAATG TCAGCGTCAG TGCCCAGCAG GCCTACATGG GGAGCACTGT 
GACCGAGAGT GCCCCATGGG ACAGTTTGGA GTTGGATGCA CACAGTTTTG TGACTGTAAG 
GGAGCTCCAT GTAACTCAGT GACCGGGAAA TGCCACTGTC CTGCAGGGCA GACAGGACAA 
CACTGTGAAA AAGAGTGTGA AGAGGGCCGT TGGGGTCTTG GCTGTGCAGA ATCATGTCCT 
TCCTGTGACA ATGGTGGAGT ATGTGATAGA CAAAACGGCA CCTGTGTTTG TTCGCCGGGA 
TTCATGGGAA ATCTCTGTCA GAATGTCTGT CCAGATGGGC ACTTTGGCCT GAGCTGCCTG 
TTGCGCTGCT CCTGTCAAAA CGAAGGGAAA TGCCACCATG TGACTGGCCT CTGTGTTTGT 
AAAGATGGCT GGATGGGACC CAACTGCGCA AAAGCTTGTG TGGCTGGACG ATGGGGGCCG 
AACTGTGCCA ACCAGTGTGA CTGCAGGAGC AGCGTGGGCA GCTGTGACCC TGTGATTGGC 
CTGTGTCATT GTGAAGCGGG CTACATGGGA CCACGCTGTG ATCAAAAGTG TCCTCAGGGT 
TTCTTCGGCC AGAGCTGCCA GCACAGATGC CAGTGTGAGA ATGGCGCAGT GTGTGAGCAT 
GTGAGCGGTG CCTGCACTTG TTTGGCTGGC TGGACTGGGA CGTATTGTGA AAAACCTTGT 
CCTGAGGGCT TTCATGGCTT GGAGTGCCAG CAGAAGTGCC AGTGCTTGAA TGGTGGCCGT 
TGTCATCCCG TGACTGGAGG CTGCCAGTGT CCATCTGGTT GGGTCGGGCC AGTTTGCAAT 
GCCACGTGTG AAGCTGGCAC CTTTGGGCCG GCCTGCAGTC AAACCTGTAG ATGCCACAAT 
AATGGCACCT GTGACCCTGC TGATGGGCGA TGTGAGTGTG GAGCAGGATG GACAGGCAGC 
AGCTGTGAAC AAGAGTGCTC AGCTGGATTG TATGGTGTGG GCTGCAACCA GCATTGCCTG 
TGTCAGAATG GAGGGTCGTG TGACCGAATC AGTGGCCACT GCACATGTGT CAGTGGGTGG 
ACAGGAGTGG CCTGTGAATT GGAATGTGCT GCGGGACACT TTGGCATGGA CTGTCAGCAC 
AAGTGTGAGT GTGTAAATGG CGGCGTGTGT GACCGTCACA GTGGCAGGTG TTTGTGCCCA 
GCAGGTTGGA CAGGCACGCA CTGTGAGACA GCATGCACTC AAGGTTTTTA TGGTCCGGGC 
TGTAAGGAGC GATGCCGGTG TGATCATAAC GCCGCATGCC ACCACATCAC TGGCACCTGC 
TTGTGTCCCG CTGGCTGGAG AGGGACTCAC TGCGAAAAGG TTTGTTTACC TGGTTCCTAT 
GGGAAGCGTT GTGCTCATCG ATGCCAGTGT CCTCATGGAA CCTCCTGTAA CCATGTGACT 
GGACAGTGTG GTTGCCCAGC GGGATTAACT GGTAGTGGCT GTGAGCGAAT GTGCCAGTCA 
GGTACGTTTG GGGTAAACTG TAATCAGGTG TGCCAGTGCT CTGAGCTCAA CCAGCTCTGC 
CACCCCGTCA CTGGTGTCTG TTACTGTGCT CCCGGATACT ATGGCAGCAA GTGTGAAACA 
GAATGTGAAG CGGGCCGTTA TGGACCCAAC TGTGAGCAGG AATGCCAGTG TCTGAATAAC 
GGTGTATGTA AAAGCAGCAC TGGCACCTGC AGCTGCCCAC CGGGATTCAT CGGACCAAAC 
TGCAACATCA CATGTCCATT AGGGCGTTAT GGTCAGGACT GTGCTGGAGT GTCTCTGTGT 
GGTGAAGGGG CTCGCAGTGA CCCTCTCACA GGCAGATGTG TGTGCAGGGC TGGTCAGCGT 
GGACGGGACT GTGGGAAAGG CTGTGCTGAG GGCTGGTTTG GAGAGAATTG TGATCAGCGC 
TGTAACTGCA GCAATGGCGG AGTGTGTGAC GCTCTTACAG GACACTGCAG CTGTACACTG 
GGCTGGACAG GAGACACCTG TGAGGAAGTG TGTCCCAGAG GACGGTATGG TTCAGACTGC 
AGTGAAGTGT GTGACTGTAA GAATAACAGC ACATGCGACC GAGTGACTGG CGTCTGTCTC 
TGCTCTCCAG GTTTCTACGG ACATCTATGT CAACATGCAT GTCCTGTTGG CTTCCATGGT 
CATCGTTGCC AGCTTCTGTG TGACTGTGGT GCAAATGCAC CTTGTGACCC TGAAACTGGC 
CGATGCCTCT GTCCTGCAGG ATACCAGGGA CTCCGCTGTG AGAGAGAGTG TGAATTGGGC 
ACATTTGGGG CTGATTGTGT CCACAGATGT GACTGTGATG GAGACACCCC ATGTGACCCT 
ATTAATGGCA GGTGTTTGTG TGCACCTGGA AAAACAGGCT CTCGCTGTGA CATAGGCTGT 
GGAGTGAACC GATTTGGACC CGACTGCTCA CAGAGATGTG AATGTCTGAA CGGAGGTCAG 
TGTGATCCCC GAAACGGCCG ATGCACGTGT CTGAACGGCT GGATCGGGCC CAGCTGTCAA 
CAAGGGCTCC TGTCTGCTAG CAAAAGTAAA AGGAAGGACT CGTCAGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295157.1
CDS	237..4607
Translation	MLSDWLRLVLVFTFWTQLVPSLAAGNPHYRYYHRNCYTCFNGYHAYNTGHQGADIDECQVHNGGCQHRCVNTRGSYYCECNPGFRLHVDGRTCIAVLSCSVNNGGCEHECVQLSPVHFQCRCRRGYQLTADGKRCRLRNPCVEKNGGCVHLCRSDGGRVVCSCHQGFMLAVDQKSCEDVNECETGLAACAHSCRNTPGSFSCICNPGYELGSDGRKCYRIEMEIVNTCDSDNGGCSHRCQHGVLGPVCSCNHGYQLAEDLKTCLDLEECEEGTSCCEQDCTNYPGGYECYCRAGYRLNADGCGCDDVDECASDRGGCEHTCQNTAGSYHCFCRFGYRLGEDRHSCISLQRSVEELPGPVVVERPLLHLALLQDYPQPLERYHDYEEDSFGELRAESTFAEKFVCLNGTFGHDCSLTCDDCSNGGYCNAEGDACDCPDGWTGILCNQTCPEGTFGKNCSFTCKCRNGGTCDPISGKCRCPPGVNGDLCQDGCPKGMYGKHCNKKCNCANNGRCHRTYGACLCDPGLYGRFCHLPCPKWSYGSGCSSECQCVQQNTLECHRRHGTCVCKPGYQGKTCNRECDSGFYGPGCRLKCQCPAGVSCHPMSGQCQRQCPAGLHGEHCDRECPMGQFGVGCTQFCDCKGAPCNSVTGKCHCPAGQTGQHCEKECEEGRWGLGCAESCPSCDNGGVCDRQNGTCVCSPGFMGNLCQNVCPDGHFGLSCLLRCSCQNEGKCHHVTGLCVCKDGWMGPNCAKACVAGRWGPNCANQCDCRSSVGSCDPVIGLCHCEAGYMGPRCDQKCPQGFFGQSCQHRCQCENGAVCEHVSGACTCLAGWTGTYCEKPCPEGFHGLECQQKCQCLNGGRCHPVTGGCQCPSGWVGPVCNATCEAGTFGPACSQTCRCHNNGTCDPADGRCECGAGWTGSSCEQECSAGLYGVGCNQHCLCQNGGSCDRISGHCTCVSGWTGVACELECAAGHFGMDCQHKCECVNGGVCDRHSGRCLCPAGWTGTHCETACTQGFYGPGCKERCRCDHNAACHHITGTCLCPAGWRGTHCEKVCLPGSYGKRCAHRCQCPHGTSCNHVTGQCGCPAGLTGSGCERMCQSGTFGVNCNQVCQCSELNQLCHPVTGVCYCAPGYYGSKCETECEAGRYGPNCEQECQCLNNGVCKSSTGTCSCPPGFIGPNCNITCPLGRYGQDCAGVSLCGEGARSDPLTGRCVCRAGQRGRDCGKGCAEGWFGENCDQRCNCSNGGVCDALTGHCSCTLGWTGDTCEEVCPRGRYGSDCSEVCDCKNNSTCDRVTGVCLCSPGFYGHLCQHACPVGFHGHRCQLLCDCGANAPCDPETGRCLCPAGYQGLRCERECELGTFGADCVHRCDCDGDTPCDPINGRCLCAPGKTGSRCDIGCGVNRFGPDCSQRCECLNGGQCDPRNGRCTCLNGWIGPSCQQGLLSASKSKRKDSSV

Target ORF information:

RefSeq Version	XM_009296882.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296882.2

ORF Insert Sequence:

ATGTTATCCG ATTGGTTGAG GTTGGTGCTT GTGTTTACCT TCTGGACTCA GCTGGTACCA 
AGTTTGGCTG CTGGAAATCC ACATTACCGC TACTATCACA GAAACTGCTA CACCTGCTTC 
AATGGATATC ATGCGTACAA CACAGGACAC CAGGGCGCAG ATATTGATGA GTGCCAGGTT 
CACAACGGAG GCTGCCAGCA CAGATGTGTG AACACTAGAG GCTCGTACTA CTGCGAGTGC 
AATCCAGGCT TTCGGCTCCA CGTTGATGGC CGCACCTGCA TAGCTGTTTT ATCCTGTTCG 
GTAAATAATG GAGGTTGTGA GCATGAATGT GTCCAGCTTT CTCCAGTTCA CTTCCAGTGC 
CGCTGCAGAC GTGGATACCA GCTTACAGCA GATGGCAAGC GCTGTAGATT GAGGAACCCA 
TGTGTGGAGA AGAATGGCGG CTGTGTGCAT TTATGTCGCA GTGATGGTGG TCGGGTTGTG 
TGCAGCTGTC ACCAAGGCTT CATGCTAGCA GTGGACCAGA AGAGCTGTGA AGATGTCAAT 
GAGTGTGAAA CAGGACTGGC AGCATGTGCA CACAGTTGCA GGAACACTCC AGGATCGTTT 
TCCTGCATCT GCAATCCAGG ATACGAGCTG GGCTCTGATG GAAGGAAATG CTACCGTATT 
GAAATGGAGA TTGTCAATAC CTGTGACTCT GATAATGGTG GCTGTTCACA TCGCTGTCAG 
CATGGTGTTC TTGGGCCTGT CTGCAGCTGT AATCATGGTT ATCAGCTCGC CGAAGACCTC 
AAAACCTGTT TGGATCTGGA GGAATGTGAG GAAGGAACGT CATGCTGTGA GCAGGATTGC 
ACAAACTACC CTGGAGGGTA TGAATGCTAC TGCAGGGCAG GTTACAGACT CAATGCAGAT 
GGATGCGGAT GTGACGATGT GGACGAGTGT GCGTCTGATA GAGGAGGCTG TGAACACACT 
TGTCAGAATA CGGCTGGCTC ATATCACTGC TTCTGTCGGT TTGGATATCG ACTTGGTGAG 
GACAGACACT CCTGCATTTC TCTCCAGCGA TCGGTTGAGG AATTGCCCGG TCCAGTGGTG 
GTTGAGCGTC CTCTCCTTCA TCTCGCTCTC CTCCAGGATT ACCCACAACC CCTGGAGCGC 
TATCATGACT ATGAGGAGGA CAGTTTTGGA GAGCTGCGGG CAGAGAGCAC CTTCGCTGAG 
AAGTTTGTGT GTTTGAATGG GACGTTTGGC CATGACTGCA GCCTGACGTG TGATGATTGC 
TCAAATGGAG GATATTGTAA TGCTGAAGGA GATGCTTGTG ACTGTCCAGA TGGGTGGACG 
GGGATCCTTT GTAACCAAAC CTGTCCAGAG GGAACATTTG GTAAAAACTG CTCCTTCACT 
TGCAAGTGCA GAAACGGGGG CACCTGTGAC CCGATTAGTG GGAAGTGCCG CTGCCCACCT 
GGAGTCAATG GAGACCTGTG CCAGGACGGC TGTCCGAAGG GAATGTATGG AAAGCACTGC 
AACAAGAAAT GTAACTGTGC TAATAACGGC CGCTGCCATC GCACATATGG AGCCTGTCTG 
TGTGATCCAG GACTCTACGG CAGGTTCTGT CACCTCCCTT GCCCCAAGTG GTCGTATGGA 
TCTGGCTGCT CATCCGAGTG TCAGTGTGTG CAACAAAACA CGCTTGAGTG CCATCGACGT 
CATGGAACGT GTGTGTGCAA ACCTGGCTAT CAGGGGAAAA CCTGCAACCG CGAGTGTGAT 
AGTGGATTTT ATGGTCCAGG ATGTAGATTA AAATGCCAGT GTCCAGCGGG AGTCTCTTGC 
CATCCCATGA GTGGGCAATG TCAGCGTCAG TGCCCAGCAG GCCTACATGG GGAGCACTGT 
GACCGAGAGT GCCCCATGGG ACAGTTTGGA GTTGGATGCA CACAGTTTTG TGACTGTAAG 
GGAGCTCCAT GTAACTCAGT GACCGGGAAA TGCCACTGTC CTGCAGGGCA GACAGGACAA 
CACTGTGAAA AAGAGTGTGA AGAGGGCCGT TGGGGTCTTG GCTGTGCAGA ATCATGTCCT 
TCCTGTGACA ATGGTGGAGT ATGTGATAGA CAAAACGGCA CCTGTGTTTG TTCGCCGGGA 
TTCATGGGAA ATCTCTGTCA GAATGTCTGT CCAGATGGGC ACTTTGGCCT GAGCTGCCTG 
TTGCGCTGCT CCTGTCAAAA CGAAGGGAAA TGCCACCATG TGACTGGCCT CTGTGTTTGT 
AAAGATGGCT GGATGGGACC CAACTGCGCA AAAGCTTGTG TGGCTGGACG ATGGGGGCCG 
AACTGTGCCA ACCAGTGTGA CTGCAGGAGC AGCGTGGGCA GCTGTGACCC TGTGATTGGC 
CTGTGTCATT GTGAAGCGGG CTACATGGGA CCACGCTGTG ATCAAAAGTG TCCTCAGGGT 
TTCTTCGGCC AGAGCTGCCA GCACAGATGC CAGTGTGAGA ATGGCGCAGT GTGTGAGCAT 
GTGAGCGGTG CCTGCACTTG TTTGGCTGGC TGGACTGGGA CGTATTGTGA AAAACCTTGT 
CCTGAGGGCT TTCATGGCTT GGAGTGCCAG CAGAAGTGCC AGTGCTTGAA TGGTGGCCGT 
TGTCATCCCG TGACTGGAGG CTGCCAGTGT CCATCTGGTT GGGTCGGGCC AGTTTGCAAT 
GCCACGTGTG AAGCTGGCAC CTTTGGGCCG GCCTGCAGTC AAACCTGTAG ATGCCACAAT 
AATGGCACCT GTGACCCTGC TGATGGGCGA TGTGAGTGTG GAGCAGGATG GACAGGCAGC 
AGCTGTGAAC AAGAGTGCTC AGCTGGATTG TATGGTGTGG GCTGCAACCA GCATTGCCTG 
TGTCAGAATG GAGGGTCGTG TGACCGAATC AGTGGCCACT GCACATGTGT CAGTGGGTGG 
ACAGGAGTGG CCTGTGAATT GGAATGTGCT GCGGGACACT TTGGCATGGA CTGTCAGCAC 
AAGTGTGAGT GTGTAAATGG CGGCGTGTGT GACCGTCACA GTGGCAGGTG TTTGTGCCCA 
GCAGGTTGGA CAGGCACGCA CTGTGAGACA GCATGCACTC AAGGTTTTTA TGGTCCGGGC 
TGTAAGGAGC GATGCCGGTG TGATCATAAC GCCGCATGCC ACCACATCAC TGGCACCTGC 
TTGTGTCCCG CTGGCTGGAG AGGGACTCAC TGCGAAAAGG TTTGTTTACC TGGTTCCTAT 
GGGAAGCGTT GTGCTCATCG ATGCCAGTGT CCTCATGGAA CCTCCTGTAA CCATGTGACT 
GGACAGTGTG GTTGCCCAGC GGGATTAACT GGTAGTGGCT GTGAGCGAAT GTGCCAGTCA 
GGTACGTTTG GGGTAAACTG TAATCAGGTG TGCCAGTGCT CTGAGCTCAA CCAGCTCTGC 
CACCCCGTCA CTGGTGTCTG TTACTGTGCT CCCGGATACT ATGGCAGCAA GTGTGAAACA 
GAATGTGAAG CGGGCCGTTA TGGACCCAAC TGTGAGCAGG AATGCCAGTG TCTGAATAAC 
GGTGTATGTA AAAGCAGCAC TGGCACCTGC AGCTGCCCAC CGGGATTCAT CGGACCAAAC 
TGCAACATCA CATGTCCATT AGGGCGTTAT GGTCAGGACT GTGCTGGAGT GTCTCTGTGT 
GGTGAAGGGG CTCGCAGTGA CCCTCTCACA GGCAGATGTG TGTGCAGGGC TGGTCAGCGT 
GGACGGGACT GTGGGAAAGG CTGTGCTGAG GGCTGGTTTG GAGAGAATTG TGATCAGCGC 
TGTAACTGCA GCAATGGCGG AGTGTGTGAC GCTCTTACAG GACACTGCAG CTGTACACTG 
GGCTGGACAG GAGACACCTG TGAGGAAGTG TGTCCCAGAG GACGGTATGG TTCAGACTGC 
AGTGAAGTGT GTGACTGTAA GAATAACAGC ACATGCGACC GAGTGACTGG CGTCTGTCTC 
TGCTCTCCAG GTTTCTACGG ACATCTATGT CAACATGCAT GTCCTGTTGG CTTCCATGGT 
CATCGTTGCC AGCTTCTGTG TGACTGTGGT GCAAATGCAC CTTGTGACCC TGAAACTGGC 
CGATGCCTCT GTCCTGCAGG ATACCAGGGA CTCCGCTGTG AGAGAGAGTG TGAATTGGGC 
ACATTTGGGG CTGATTGTGT CCACAGATGT GACTGTGATG GAGACACCCC ATGTGACCCT 
ATTAATGGCA GGTGTTTGTG TGCACCTGGA AAAACAGGCT CTCGCTGTGA CATAGGCTGT 
GGAGTGAACC GATTTGGACC CGACTGCTCA CAGAGATGTG AATGTCTGAA CGGAGGTCAG 
TGTGATCCCC GAAACGGCCG ATGCACGTGT CTGAACGGCT GGATCGGGCC CAGCTGTCAA 
CAAGGGCTCC TGTCTGCTAG CAAAAGTAAA AGGAAGGACT CGTCAGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

megf6a ( XM_009296882.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295157.1 Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X2, mRNA.

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CloneID	ODa44516
Clone ID Related Accession (Same CDS sequence)	XM_009296882.2 , XM_009296882.3
Accession Version	XM_009296882.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	multiple epidermal growth factor-like domains protein 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296882.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCCG ATTGGTTGAG GTTGGTGCTT GTGTTTACCT TCTGGACTCA GCTGGTACCA 
AGTTTGGCTG CTGGAAATCC ACATTACCGC TACTATCACA GAAACTGCTA CACCTGCTTC 
AATGGATATC ATGCGTACAA CACAGGACAC CAGGGCGCAG ATATTGATGA GTGCCAGGTT 
CACAACGGAG GCTGCCAGCA CAGATGTGTG AACACTAGAG GCTCGTACTA CTGCGAGTGC 
AATCCAGGCT TTCGGCTCCA CGTTGATGGC CGCACCTGCA TAGCTGTTTT ATCCTGTTCG 
GTAAATAATG GAGGTTGTGA GCATGAATGT GTCCAGCTTT CTCCAGTTCA CTTCCAGTGC 
CGCTGCAGAC GTGGATACCA GCTTACAGCA GATGGCAAGC GCTGTAGATT GAGGAACCCA 
TGTGTGGAGA AGAATGGCGG CTGTGTGCAT TTATGTCGCA GTGATGGTGG TCGGGTTGTG 
TGCAGCTGTC ACCAAGGCTT CATGCTAGCA GTGGACCAGA AGAGCTGTGA AGATGTCAAT 
GAGTGTGAAA CAGGACTGGC AGCATGTGCA CACAGTTGCA GGAACACTCC AGGATCGTTT 
TCCTGCATCT GCAATCCAGG ATACGAGCTG GGCTCTGATG GAAGGAAATG CTACCGTATT 
GAAATGGAGA TTGTCAATAC CTGTGACTCT GATAATGGTG GCTGTTCACA TCGCTGTCAG 
CATGGTGTTC TTGGGCCTGT CTGCAGCTGT AATCATGGTT ATCAGCTCGC CGAAGACCTC 
AAAACCTGTT TGGATCTGGA GGAATGTGAG GAAGGAACGT CATGCTGTGA GCAGGATTGC 
ACAAACTACC CTGGAGGGTA TGAATGCTAC TGCAGGGCAG GTTACAGACT CAATGCAGAT 
GGATGCGGAT GTGACGATGT GGACGAGTGT GCGTCTGATA GAGGAGGCTG TGAACACACT 
TGTCAGAATA CGGCTGGCTC ATATCACTGC TTCTGTCGGT TTGGATATCG ACTTGGTGAG 
GACAGACACT CCTGCATTTC TCTCCAGCGA TCGGTTGAGG AATTGCCCGG TCCAGTGGTG 
GTTGAGCGTC CTCTCCTTCA TCTCGCTCTC CTCCAGGATT ACCCACAACC CCTGGAGCGC 
TATCATGACT ATGAGGAGGA CAGTTTTGGA GAGCTGCGGG CAGAGAGCAC CTTCGCTGAG 
AAGTTTGTGT GTTTGAATGG GACGTTTGGC CATGACTGCA GCCTGACGTG TGATGATTGC 
TCAAATGGAG GATATTGTAA TGCTGAAGGA GATGCTTGTG ACTGTCCAGA TGGGTGGACG 
GGGATCCTTT GTAACCAAAC CTGTCCAGAG GGAACATTTG GTAAAAACTG CTCCTTCACT 
TGCAAGTGCA GAAACGGGGG CACCTGTGAC CCGATTAGTG GGAAGTGCCG CTGCCCACCT 
GGAGTCAATG GAGACCTGTG CCAGGACGGC TGTCCGAAGG GAATGTATGG AAAGCACTGC 
AACAAGAAAT GTAACTGTGC TAATAACGGC CGCTGCCATC GCACATATGG AGCCTGTCTG 
TGTGATCCAG GACTCTACGG CAGGTTCTGT CACCTCCCTT GCCCCAAGTG GTCGTATGGA 
TCTGGCTGCT CATCCGAGTG TCAGTGTGTG CAACAAAACA CGCTTGAGTG CCATCGACGT 
CATGGAACGT GTGTGTGCAA ACCTGGCTAT CAGGGGAAAA CCTGCAACCG CGAGTGTGAT 
AGTGGATTTT ATGGTCCAGG ATGTAGATTA AAATGCCAGT GTCCAGCGGG AGTCTCTTGC 
CATCCCATGA GTGGGCAATG TCAGCGTCAG TGCCCAGCAG GCCTACATGG GGAGCACTGT 
GACCGAGAGT GCCCCATGGG ACAGTTTGGA GTTGGATGCA CACAGTTTTG TGACTGTAAG 
GGAGCTCCAT GTAACTCAGT GACCGGGAAA TGCCACTGTC CTGCAGGGCA GACAGGACAA 
CACTGTGAAA AAGAGTGTGA AGAGGGCCGT TGGGGTCTTG GCTGTGCAGA ATCATGTCCT 
TCCTGTGACA ATGGTGGAGT ATGTGATAGA CAAAACGGCA CCTGTGTTTG TTCGCCGGGA 
TTCATGGGAA ATCTCTGTCA GAATGTCTGT CCAGATGGGC ACTTTGGCCT GAGCTGCCTG 
TTGCGCTGCT CCTGTCAAAA CGAAGGGAAA TGCCACCATG TGACTGGCCT CTGTGTTTGT 
AAAGATGGCT GGATGGGACC CAACTGCGCA AAAGCTTGTG TGGCTGGACG ATGGGGGCCG 
AACTGTGCCA ACCAGTGTGA CTGCAGGAGC AGCGTGGGCA GCTGTGACCC TGTGATTGGC 
CTGTGTCATT GTGAAGCGGG CTACATGGGA CCACGCTGTG ATCAAAAGTG TCCTCAGGGT 
TTCTTCGGCC AGAGCTGCCA GCACAGATGC CAGTGTGAGA ATGGCGCAGT GTGTGAGCAT 
GTGAGCGGTG CCTGCACTTG TTTGGCTGGC TGGACTGGGA CGTATTGTGA AAAACCTTGT 
CCTGAGGGCT TTCATGGCTT GGAGTGCCAG CAGAAGTGCC AGTGCTTGAA TGGTGGCCGT 
TGTCATCCCG TGACTGGAGG CTGCCAGTGT CCATCTGGTT GGGTCGGGCC AGTTTGCAAT 
GCCACGTGTG AAGCTGGCAC CTTTGGGCCG GCCTGCAGTC AAACCTGTAG ATGCCACAAT 
AATGGCACCT GTGACCCTGC TGATGGGCGA TGTGAGTGTG GAGCAGGATG GACAGGCAGC 
AGCTGTGAAC AAGAGTGCTC AGCTGGATTG TATGGTGTGG GCTGCAACCA GCATTGCCTG 
TGTCAGAATG GAGGGTCGTG TGACCGAATC AGTGGCCACT GCACATGTGT CAGTGGGTGG 
ACAGGAGTGG CCTGTGAATT GGAATGTGCT GCGGGACACT TTGGCATGGA CTGTCAGCAC 
AAGTGTGAGT GTGTAAATGG CGGCGTGTGT GACCGTCACA GTGGCAGGTG TTTGTGCCCA 
GCAGGTTGGA CAGGCACGCA CTGTGAGACA GCATGCACTC AAGGTTTTTA TGGTCCGGGC 
TGTAAGGAGC GATGCCGGTG TGATCATAAC GCCGCATGCC ACCACATCAC TGGCACCTGC 
TTGTGTCCCG CTGGCTGGAG AGGGACTCAC TGCGAAAAGG TTTGTTTACC TGGTTCCTAT 
GGGAAGCGTT GTGCTCATCG ATGCCAGTGT CCTCATGGAA CCTCCTGTAA CCATGTGACT 
GGACAGTGTG GTTGCCCAGC GGGATTAACT GGTAGTGGCT GTGAGCGAAT GTGCCAGTCA 
GGTACGTTTG GGGTAAACTG TAATCAGGTG TGCCAGTGCT CTGAGCTCAA CCAGCTCTGC 
CACCCCGTCA CTGGTGTCTG TTACTGTGCT CCCGGATACT ATGGCAGCAA GTGTGAAACA 
GAATGTGAAG CGGGCCGTTA TGGACCCAAC TGTGAGCAGG AATGCCAGTG TCTGAATAAC 
GGTGTATGTA AAAGCAGCAC TGGCACCTGC AGCTGCCCAC CGGGATTCAT CGGACCAAAC 
TGCAACATCA CATGTCCATT AGGGCGTTAT GGTCAGGACT GTGCTGGAGT GTCTCTGTGT 
GGTGAAGGGG CTCGCAGTGA CCCTCTCACA GGCAGATGTG TGTGCAGGGC TGGTCAGCGT 
GGACGGGACT GTGGGAAAGG CTGTGCTGAG GGCTGGTTTG GAGAGAATTG TGATCAGCGC 
TGTAACTGCA GCAATGGCGG AGTGTGTGAC GCTCTTACAG GACACTGCAG CTGTACACTG 
GGCTGGACAG GAGACACCTG TGAGGAAGTG TGTCCCAGAG GACGGTATGG TTCAGACTGC 
AGTGAAGTGT GTGACTGTAA GAATAACAGC ACATGCGACC GAGTGACTGG CGTCTGTCTC 
TGCTCTCCAG GTTTCTACGG ACATCTATGT CAACATGCAT GTCCTGTTGG CTTCCATGGT 
CATCGTTGCC AGCTTCTGTG TGACTGTGGT GCAAATGCAC CTTGTGACCC TGAAACTGGC 
CGATGCCTCT GTCCTGCAGG ATACCAGGGA CTCCGCTGTG AGAGAGAGTG TGAATTGGGC 
ACATTTGGGG CTGATTGTGT CCACAGATGT GACTGTGATG GAGACACCCC ATGTGACCCT 
ATTAATGGCA GGTGTTTGTG TGCACCTGGA AAAACAGGCT CTCGCTGTGA CATAGGCTGT 
GGAGTGAACC GATTTGGACC CGACTGCTCA CAGAGATGTG AATGTCTGAA CGGAGGTCAG 
TGTGATCCCC GAAACGGCCG ATGCACGTGT CTGAACGGCT GGATCGGGCC CAGCTGTCAA 
CAAGGGCTCC TGTCTGCTAG CAAAAGTAAA AGGAAGGACT CGTCAGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295157.1
CDS	243..4613
Translation	MLSDWLRLVLVFTFWTQLVPSLAAGNPHYRYYHRNCYTCFNGYHAYNTGHQGADIDECQVHNGGCQHRCVNTRGSYYCECNPGFRLHVDGRTCIAVLSCSVNNGGCEHECVQLSPVHFQCRCRRGYQLTADGKRCRLRNPCVEKNGGCVHLCRSDGGRVVCSCHQGFMLAVDQKSCEDVNECETGLAACAHSCRNTPGSFSCICNPGYELGSDGRKCYRIEMEIVNTCDSDNGGCSHRCQHGVLGPVCSCNHGYQLAEDLKTCLDLEECEEGTSCCEQDCTNYPGGYECYCRAGYRLNADGCGCDDVDECASDRGGCEHTCQNTAGSYHCFCRFGYRLGEDRHSCISLQRSVEELPGPVVVERPLLHLALLQDYPQPLERYHDYEEDSFGELRAESTFAEKFVCLNGTFGHDCSLTCDDCSNGGYCNAEGDACDCPDGWTGILCNQTCPEGTFGKNCSFTCKCRNGGTCDPISGKCRCPPGVNGDLCQDGCPKGMYGKHCNKKCNCANNGRCHRTYGACLCDPGLYGRFCHLPCPKWSYGSGCSSECQCVQQNTLECHRRHGTCVCKPGYQGKTCNRECDSGFYGPGCRLKCQCPAGVSCHPMSGQCQRQCPAGLHGEHCDRECPMGQFGVGCTQFCDCKGAPCNSVTGKCHCPAGQTGQHCEKECEEGRWGLGCAESCPSCDNGGVCDRQNGTCVCSPGFMGNLCQNVCPDGHFGLSCLLRCSCQNEGKCHHVTGLCVCKDGWMGPNCAKACVAGRWGPNCANQCDCRSSVGSCDPVIGLCHCEAGYMGPRCDQKCPQGFFGQSCQHRCQCENGAVCEHVSGACTCLAGWTGTYCEKPCPEGFHGLECQQKCQCLNGGRCHPVTGGCQCPSGWVGPVCNATCEAGTFGPACSQTCRCHNNGTCDPADGRCECGAGWTGSSCEQECSAGLYGVGCNQHCLCQNGGSCDRISGHCTCVSGWTGVACELECAAGHFGMDCQHKCECVNGGVCDRHSGRCLCPAGWTGTHCETACTQGFYGPGCKERCRCDHNAACHHITGTCLCPAGWRGTHCEKVCLPGSYGKRCAHRCQCPHGTSCNHVTGQCGCPAGLTGSGCERMCQSGTFGVNCNQVCQCSELNQLCHPVTGVCYCAPGYYGSKCETECEAGRYGPNCEQECQCLNNGVCKSSTGTCSCPPGFIGPNCNITCPLGRYGQDCAGVSLCGEGARSDPLTGRCVCRAGQRGRDCGKGCAEGWFGENCDQRCNCSNGGVCDALTGHCSCTLGWTGDTCEEVCPRGRYGSDCSEVCDCKNNSTCDRVTGVCLCSPGFYGHLCQHACPVGFHGHRCQLLCDCGANAPCDPETGRCLCPAGYQGLRCERECELGTFGADCVHRCDCDGDTPCDPINGRCLCAPGKTGSRCDIGCGVNRFGPDCSQRCECLNGGQCDPRNGRCTCLNGWIGPSCQQGLLSASKSKRKDSSV

Target ORF information:

RefSeq Version	XM_009296882.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296882.3

ORF Insert Sequence:

ATGTTATCCG ATTGGTTGAG GTTGGTGCTT GTGTTTACCT TCTGGACTCA GCTGGTACCA 
AGTTTGGCTG CTGGAAATCC ACATTACCGC TACTATCACA GAAACTGCTA CACCTGCTTC 
AATGGATATC ATGCGTACAA CACAGGACAC CAGGGCGCAG ATATTGATGA GTGCCAGGTT 
CACAACGGAG GCTGCCAGCA CAGATGTGTG AACACTAGAG GCTCGTACTA CTGCGAGTGC 
AATCCAGGCT TTCGGCTCCA CGTTGATGGC CGCACCTGCA TAGCTGTTTT ATCCTGTTCG 
GTAAATAATG GAGGTTGTGA GCATGAATGT GTCCAGCTTT CTCCAGTTCA CTTCCAGTGC 
CGCTGCAGAC GTGGATACCA GCTTACAGCA GATGGCAAGC GCTGTAGATT GAGGAACCCA 
TGTGTGGAGA AGAATGGCGG CTGTGTGCAT TTATGTCGCA GTGATGGTGG TCGGGTTGTG 
TGCAGCTGTC ACCAAGGCTT CATGCTAGCA GTGGACCAGA AGAGCTGTGA AGATGTCAAT 
GAGTGTGAAA CAGGACTGGC AGCATGTGCA CACAGTTGCA GGAACACTCC AGGATCGTTT 
TCCTGCATCT GCAATCCAGG ATACGAGCTG GGCTCTGATG GAAGGAAATG CTACCGTATT 
GAAATGGAGA TTGTCAATAC CTGTGACTCT GATAATGGTG GCTGTTCACA TCGCTGTCAG 
CATGGTGTTC TTGGGCCTGT CTGCAGCTGT AATCATGGTT ATCAGCTCGC CGAAGACCTC 
AAAACCTGTT TGGATCTGGA GGAATGTGAG GAAGGAACGT CATGCTGTGA GCAGGATTGC 
ACAAACTACC CTGGAGGGTA TGAATGCTAC TGCAGGGCAG GTTACAGACT CAATGCAGAT 
GGATGCGGAT GTGACGATGT GGACGAGTGT GCGTCTGATA GAGGAGGCTG TGAACACACT 
TGTCAGAATA CGGCTGGCTC ATATCACTGC TTCTGTCGGT TTGGATATCG ACTTGGTGAG 
GACAGACACT CCTGCATTTC TCTCCAGCGA TCGGTTGAGG AATTGCCCGG TCCAGTGGTG 
GTTGAGCGTC CTCTCCTTCA TCTCGCTCTC CTCCAGGATT ACCCACAACC CCTGGAGCGC 
TATCATGACT ATGAGGAGGA CAGTTTTGGA GAGCTGCGGG CAGAGAGCAC CTTCGCTGAG 
AAGTTTGTGT GTTTGAATGG GACGTTTGGC CATGACTGCA GCCTGACGTG TGATGATTGC 
TCAAATGGAG GATATTGTAA TGCTGAAGGA GATGCTTGTG ACTGTCCAGA TGGGTGGACG 
GGGATCCTTT GTAACCAAAC CTGTCCAGAG GGAACATTTG GTAAAAACTG CTCCTTCACT 
TGCAAGTGCA GAAACGGGGG CACCTGTGAC CCGATTAGTG GGAAGTGCCG CTGCCCACCT 
GGAGTCAATG GAGACCTGTG CCAGGACGGC TGTCCGAAGG GAATGTATGG AAAGCACTGC 
AACAAGAAAT GTAACTGTGC TAATAACGGC CGCTGCCATC GCACATATGG AGCCTGTCTG 
TGTGATCCAG GACTCTACGG CAGGTTCTGT CACCTCCCTT GCCCCAAGTG GTCGTATGGA 
TCTGGCTGCT CATCCGAGTG TCAGTGTGTG CAACAAAACA CGCTTGAGTG CCATCGACGT 
CATGGAACGT GTGTGTGCAA ACCTGGCTAT CAGGGGAAAA CCTGCAACCG CGAGTGTGAT 
AGTGGATTTT ATGGTCCAGG ATGTAGATTA AAATGCCAGT GTCCAGCGGG AGTCTCTTGC 
CATCCCATGA GTGGGCAATG TCAGCGTCAG TGCCCAGCAG GCCTACATGG GGAGCACTGT 
GACCGAGAGT GCCCCATGGG ACAGTTTGGA GTTGGATGCA CACAGTTTTG TGACTGTAAG 
GGAGCTCCAT GTAACTCAGT GACCGGGAAA TGCCACTGTC CTGCAGGGCA GACAGGACAA 
CACTGTGAAA AAGAGTGTGA AGAGGGCCGT TGGGGTCTTG GCTGTGCAGA ATCATGTCCT 
TCCTGTGACA ATGGTGGAGT ATGTGATAGA CAAAACGGCA CCTGTGTTTG TTCGCCGGGA 
TTCATGGGAA ATCTCTGTCA GAATGTCTGT CCAGATGGGC ACTTTGGCCT GAGCTGCCTG 
TTGCGCTGCT CCTGTCAAAA CGAAGGGAAA TGCCACCATG TGACTGGCCT CTGTGTTTGT 
AAAGATGGCT GGATGGGACC CAACTGCGCA AAAGCTTGTG TGGCTGGACG ATGGGGGCCG 
AACTGTGCCA ACCAGTGTGA CTGCAGGAGC AGCGTGGGCA GCTGTGACCC TGTGATTGGC 
CTGTGTCATT GTGAAGCGGG CTACATGGGA CCACGCTGTG ATCAAAAGTG TCCTCAGGGT 
TTCTTCGGCC AGAGCTGCCA GCACAGATGC CAGTGTGAGA ATGGCGCAGT GTGTGAGCAT 
GTGAGCGGTG CCTGCACTTG TTTGGCTGGC TGGACTGGGA CGTATTGTGA AAAACCTTGT 
CCTGAGGGCT TTCATGGCTT GGAGTGCCAG CAGAAGTGCC AGTGCTTGAA TGGTGGCCGT 
TGTCATCCCG TGACTGGAGG CTGCCAGTGT CCATCTGGTT GGGTCGGGCC AGTTTGCAAT 
GCCACGTGTG AAGCTGGCAC CTTTGGGCCG GCCTGCAGTC AAACCTGTAG ATGCCACAAT 
AATGGCACCT GTGACCCTGC TGATGGGCGA TGTGAGTGTG GAGCAGGATG GACAGGCAGC 
AGCTGTGAAC AAGAGTGCTC AGCTGGATTG TATGGTGTGG GCTGCAACCA GCATTGCCTG 
TGTCAGAATG GAGGGTCGTG TGACCGAATC AGTGGCCACT GCACATGTGT CAGTGGGTGG 
ACAGGAGTGG CCTGTGAATT GGAATGTGCT GCGGGACACT TTGGCATGGA CTGTCAGCAC 
AAGTGTGAGT GTGTAAATGG CGGCGTGTGT GACCGTCACA GTGGCAGGTG TTTGTGCCCA 
GCAGGTTGGA CAGGCACGCA CTGTGAGACA GCATGCACTC AAGGTTTTTA TGGTCCGGGC 
TGTAAGGAGC GATGCCGGTG TGATCATAAC GCCGCATGCC ACCACATCAC TGGCACCTGC 
TTGTGTCCCG CTGGCTGGAG AGGGACTCAC TGCGAAAAGG TTTGTTTACC TGGTTCCTAT 
GGGAAGCGTT GTGCTCATCG ATGCCAGTGT CCTCATGGAA CCTCCTGTAA CCATGTGACT 
GGACAGTGTG GTTGCCCAGC GGGATTAACT GGTAGTGGCT GTGAGCGAAT GTGCCAGTCA 
GGTACGTTTG GGGTAAACTG TAATCAGGTG TGCCAGTGCT CTGAGCTCAA CCAGCTCTGC 
CACCCCGTCA CTGGTGTCTG TTACTGTGCT CCCGGATACT ATGGCAGCAA GTGTGAAACA 
GAATGTGAAG CGGGCCGTTA TGGACCCAAC TGTGAGCAGG AATGCCAGTG TCTGAATAAC 
GGTGTATGTA AAAGCAGCAC TGGCACCTGC AGCTGCCCAC CGGGATTCAT CGGACCAAAC 
TGCAACATCA CATGTCCATT AGGGCGTTAT GGTCAGGACT GTGCTGGAGT GTCTCTGTGT 
GGTGAAGGGG CTCGCAGTGA CCCTCTCACA GGCAGATGTG TGTGCAGGGC TGGTCAGCGT 
GGACGGGACT GTGGGAAAGG CTGTGCTGAG GGCTGGTTTG GAGAGAATTG TGATCAGCGC 
TGTAACTGCA GCAATGGCGG AGTGTGTGAC GCTCTTACAG GACACTGCAG CTGTACACTG 
GGCTGGACAG GAGACACCTG TGAGGAAGTG TGTCCCAGAG GACGGTATGG TTCAGACTGC 
AGTGAAGTGT GTGACTGTAA GAATAACAGC ACATGCGACC GAGTGACTGG CGTCTGTCTC 
TGCTCTCCAG GTTTCTACGG ACATCTATGT CAACATGCAT GTCCTGTTGG CTTCCATGGT 
CATCGTTGCC AGCTTCTGTG TGACTGTGGT GCAAATGCAC CTTGTGACCC TGAAACTGGC 
CGATGCCTCT GTCCTGCAGG ATACCAGGGA CTCCGCTGTG AGAGAGAGTG TGAATTGGGC 
ACATTTGGGG CTGATTGTGT CCACAGATGT GACTGTGATG GAGACACCCC ATGTGACCCT 
ATTAATGGCA GGTGTTTGTG TGCACCTGGA AAAACAGGCT CTCGCTGTGA CATAGGCTGT 
GGAGTGAACC GATTTGGACC CGACTGCTCA CAGAGATGTG AATGTCTGAA CGGAGGTCAG 
TGTGATCCCC GAAACGGCCG ATGCACGTGT CTGAACGGCT GGATCGGGCC CAGCTGTCAA 
CAAGGGCTCC TGTCTGCTAG CAAAAGTAAA AGGAAGGACT CGTCAGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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megf6a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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